Fibroblasts which occur in the CORNEAL STROMA.
The lamellated connective tissue constituting the thickest layer of the cornea between the Bowman and Descemet membranes.
The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
Restoration of integrity to traumatized tissue.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Methods for maintaining or growing CELLS in vitro.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.
Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.
A group of differentiation surface antigens, among the first to be discovered on thymocytes and T-lymphocytes. Originally identified in the mouse, they are also found in other species including humans, and are expressed on brain neurons and other cells.
Spindle-shaped cells with characteristic CONTRACTILE PROTEINS and structures that contribute to the WOUND HEALING process. They occur in GRANULATION TISSUE and also in pathological processes such as FIBROSIS.
Bilateral hereditary disorders of the cornea, usually autosomal dominant, which may be present at birth but more frequently develop during adolescence and progress slowly throughout life. Central macular dystrophy is transmitted as an autosomal recessive defect.
An enzyme that, in the pathway of cholesterol biosynthesis, catalyzes the condensation of isopentenyl pyrophosphate and dimethylallylpyrophosphate to yield pyrophosphate and geranylpyrophosphate. The enzyme then catalyzes the condensation of the latter compound with another molecule of isopentenyl pyrophosphate to yield pyrophosphate and farnesylpyrophosphate. EC 2.5.1.1.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
A family of transcription factors characterized by the presence of a bipartite DNA-binding domain known as the POU domain. The POU domain contains two subdomains, a POU-specific domain and a POU-homeodomain. The POU domain was originally identified as a region of approximately 150 amino acids shared between the Pit-1, Oct-1, Oct-2, and Unc-86 transcription factors.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Genes that encode highly conserved TRANSCRIPTION FACTORS that control positional identity of cells (BODY PATTERNING) and MORPHOGENESIS throughout development. Their sequences contain a 180 nucleotide sequence designated the homeobox, so called because mutations of these genes often results in homeotic transformations, in which one body structure replaces another. The proteins encoded by homeobox genes are called HOMEODOMAIN PROTEINS.
A local anesthetic of the ester type that has a rapid onset of action and a longer duration of action than procaine hydrochloride. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1017)
A syndrome characterized by indifference to PAIN despite the ability to distinguish noxious from non-noxious stimuli. Absent corneal reflexes and INTELLECTUAL DISABILITY may be associated. Familial forms with autosomal recessive and autosomal dominant patterns of inheritance have been described. (Adams et al., Principles of Neurology, 6th ed, p1343)
The application of drug preparations to the surfaces of the body, especially the skin (ADMINISTRATION, CUTANEOUS) or mucous membranes. This method of treatment is used to avoid systemic side effects when high doses are required at a localized area or as an alternative systemic administration route, to avoid hepatic processing for example.
A flat, flexible strip of material used to cover or fasten together damaged tissue.
Uptake of substances through the SKIN.

Insulin growth factor promotes human corneal fibroblast network formation in vitro. (1/66)

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Polyethylenimine-conjugated gold nanoparticles: Gene transfer potential and low toxicity in the cornea. (2/66)

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Synergistic induction of eotaxin and VCAM-1 expression in human corneal fibroblasts by staphylococcal peptidoglycan and either IL-4 or IL-13. (3/66)

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An explanation for the central to peripheral thickness variation in the mouse cornea. (4/66)

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Localization of thrombospondin-1 and myofibroblasts during corneal wound repair. (5/66)

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Hydroxyapatite for keratoprosthesis biointegration. (6/66)

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Assessment of corneal thickness and keratocyte density in a rabbit model of laser in situ keratomileusis using scanning laser confocal microscopy. (7/66)

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Coxsackievirus A24 variant uses sialic acid-containing O-linked glycoconjugates as cellular receptors on human ocular cells. (8/66)

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The results of the JNK inhibitor studies were verified using DsiRNA to knockdown JNK1 and JNK2 in the cells. FGF-2/HS-induced activation of keratocytes, transfected with scrambled DsiRNA (controls), resulted in a 140% ± 40% and a 130% ± 20% increase in JNK1 and JNK2 mRNA levels, respectively (Fig. 5). The levels of lumican and keratocan in these cells were reduced by 65% ± 3% and 45% ± 15%, respectively. When nonactivated keratocytes were transfected with JNK1/2 DsiRNA, the levels of JNK1 and JNK2 mRNAs were reduced by 53% ± 6% and 66% ± 7%, respectively. The levels of lumican and keratocan in these cells were 27% ± 5% and 44% ± 6% higher, respectively, than those in corresponding scrambled DsiRNA-transfected controls. Similarly, when the keratocytes, transfected with JNK1/2 DsiRNA, were activated with FGF-2/HS, the levels of JNK1 and JNK2 mRNAs were reduced by 75% ± 13% and 57% ± 8%, respectively. The levels of lumican and keratocan in these cells were 70% ± 20% and 100% ± 10% ...
After corneal injury, keratocytes become activated and transform into repair phenotypes-corneal fibroblasts or myofibroblasts, however, these important cells are difficult to identify histologically, compromising studies of stromal wound healing. Recent studies indicate that expression of the cell surface protein, Thy-1, is induced in fibroblast populations associated with wound healing and fibrosis in other tissues. We investigated whether keratocyte transformation to either repair-associated phenotype induced Thy-1 expression. Human corneal keratocytes were isolated by collagenase digestion. The cells were either processed immediately (i.e. freshly isolated keratocytes) or were cultured in the presence of 10% fetal bovine serum or transforming growth factor-β to induce transformation to the corneal fibroblast and myofibroblast phenotypes, respectively. Thy-1 mRNA and protein expression by freshly isolated keratocytes and corneal fibroblasts were assessed by RT-PCR and Western blotting. mRNA ...
Purpose : Vascular endothelial growth factor receptor 3 (VEGFR3) regulates the growth and differentiation of blood and lymphatic vessels. We hypothesized that matrix metalloproteinase 14 (MMP14)-modulated VEGFR3 expression in the corneal epithelium may influence corneal avascularity. Methods : VEGFR3 expression was compared between wild-type and MMP14-deficient (MMP14 Δexon4) corneal epithelial cells and keratocytes. VEGFR3 protein and mRNA expression were measured by Western blot and quantitative RT-PCR analyses, respectively. Potential cleavage of VEGFR3 by catalytic MMP14 was examined in an in vitro proteolysis experiment. Mass proteomic analysis was used to identify potential nuclear proteins differentially expressed in MMP14 Δexon4 and WT corneal epithelial cells. Results : VEGFR3 protein expression was much higher on MMP14 Δexon4 corneal epithelial cells than on wild-type cells. Additionally, quantitative RT-PCR analysis showed that VEGFR3 gene expression was highly induced in MMP14 ...
TY - CONF. T1 - Proteome analysis of serum-cultured human corneal fibroblasts. AU - Karring, Henrik AU - Thøgersen, Ida B. AU - Klintworth, Gordon K. AU - Enghild, Jan J. AU - Møller-Pedersen, Torben. PY - 2004. Y1 - 2004. M3 - Poster. Y2 - 8 November 2004 through 12 November 2004. ER - ...
All keratocytes participate in sphere formation. A: Freshly isolated primary keratocytes were cultured in polyHEMA-coated dishes either in Advanced-DMEM (ADV, d
Lamella of keratocytes generate a small rearward traction force against the micromachined substratum. Bar, 5 μm. Once the lamella of a keratocyte is over the m
Gr 7 Up — For sophomore Lara Kelly, things are finally looking up—shes feeling more confident after losing weight and she made the varsity cheerleading team, which she never would have imagined two years earlier when she was overweight and severely depressed. Best of all, Lara has caught the attention of a cute guy on Facebook, and he has been hinting at asking her to the homecoming dance. But when she sees horrible comments from her crush on social media, she spirals into a dangerous mental state and suicide seems like the only escape. Bree is Laras former best friend from middle school, but they drifted apart when Bree couldnt take Laras depression and self-involvement. The new Lara is suddenly getting everything that Bree is supposed to have—the popularity, and even the spot on the cheer team. Sydney and Liam are the younger siblings, who are caught up in the horror of a tragic event, and trying to figure out how to cope with their siblings issues while living their own ...
1. Jester J, Ho-Chang J. Modulation of cultured corneal keratocyte phenotype by growth factors/cytokines control in vitro contractility and extracellular matrix contraction. Exp Eye Res. 2003;77(5):581-92.. 2. Myrna KE, Pot SA, Murphy CJ. Meet the corneal myofibroblast: the role of myofibroblast transformation in corneal wound healing and pathology. Vet Ophthalmol. 2009;12 Suppl 1:25-7.. 3. Remingto, L. Cornea and Sclera. In: Clinical Anatomy of the Visual System. 3rd ed. St. Louis, Mo: Elsevier, 2012.. 4. Alio J, Rodriguez A, Ferreira-oliveira R, et al. Treatment of dry eye disease with autologous platelet-rich plasma: a prospective, interventional, non-randomized study. Ophthalmol Ther. 2017;6(2):285-93.. 5. Alio JL, Rodriguez AE, Abdelghany AA, et al. Autologous platelet-rich plasma eye drops for the treatment of post-LASIK chronic ocular surface syndrome. J Ophthalmol. 2017;2017:2457620.. 6. Anitua E, Muruzabal F, De la fuente M, et al. Plasma rich in growth factors for the treatment of ...
The human cornea contains stem cells that can be induced to express markers consistent with multipotency in cell culture; however, there have been no studies demonstrating that human corneal keratocytes are multipotent. The objective of this study is to examine the potential of human fetal keratocytes (HFKs) to differentiate into neural crest-derived tissues when challenged in an embryonic environment. HFKs were injected bilaterally into the cranial mesenchyme adjacent to the neural tube and the periocular mesenchyme in chick embryos at embryonic days 1.5 and 3, respectively. The injected keratocytes were detected by immunofluorescence using the human cell-specific marker, HuNu. HuNu-positive keratocytes injected along the neural crest pathway were localized adjacent to HNK-1-positive migratory host neural crest cells and in the cardiac cushion mesenchyme. The HuNu-positive cells transformed into neural crest derivatives such as smooth muscle in cranial blood vessels, stromal keratocytes, and ...
Purpose: Accidental or surgical-induced trauma (e.g., refractive surgery) to the corneal epithelium results in keratocyte death immediately below the wound. Subsequent recovery of keratocytes during healing often remains ...
Spontaneous symmetry breaking and motility initiation of keratocytes. Phase-contrast time-lapse video of a keratocyte spontaneously breaking symmetry ...
A total of 18 patients were recruited with similar bilateral refraction. The patients underwent SMILE in one eye and FS-LASIK in the other eye. After detailed clinical examination, patients underwent imaging on Pentacam HR and Corvis-ST before and after surgery (1, 3 and 6month). Corneal tomography from Pentacam-HR was used to construct 3-D corneal model and mesh.The I-FEM derived the best fit material properties to describe the corneal deformation measured by Corvis-ST.Using the computed properties, stress strain analogue for each measurement was formulated by simulating intraocular pressure(IOP; 0-60mmHg).The displacement of the corneal apex was calculated from stress-strain analogue ...
wst1 blank absorbance problem - posted in Tissue and Cell Culture: Hi everyone, Recently, Ive tried wst1 assay for the first time. After treating the cells with chemical, i added 100 ul fresh DMEM containing 10 ul wst1 and incubated for 3 hours. Ive measured the absorbance in 355-460 interval (this was the closest filter pair to 450 nm -that I could choose in our reader-). Unexpectedly, I got the maximum absorbance values in my blank samples (DMEM+wst1; without cells) and positive controls...
The Rx Review: Reporting on Fitness and CrossFit News, John Michael Bric This week we take a moment to review the Herculin Extreme Pre-Workout supplement by EBN Sports Nutrition. Item: Herculin Extreme Pre-Workout Price: $54 USD (450 grams) Retailer: EBN Sports Nutrition Purchase: www.EBNSports.com The current supplement market is flooded with hundreds of different pre-workout products. Many of which claim to…
TY - JOUR. T1 - Quantitative assessment of anteroposterior keratocyte density in the normal rabbit cornea. AU - Petroll, W. M.. AU - Boettcher, K.. AU - Barry, P.. AU - Cavanagh, D.. AU - Jester, J. V.. PY - 1995. Y1 - 1995. N2 - The anteroposterior keratocyte density distribution in the rabbit cornea was measured. Unsectioned tissue blocks from the central cornea of five rabbits were stained with propidium iodide and imaged using a Leica laser scanning confocal microscope. A z-series of images was acquired in each sample, from anterior to posterior stroma in either 3- or 8-μm steps. Software was developed to allow interactive marking of the keratocyte nuclei within each section of the z-series and for calculating cell density. For convenience, cell density was expressed as the number of cells per corneal volume element (CVE), where CVE is a newly defined volume unit with x, y, and z dimensions of 250, 250, and 10 μm, respectively. The calculated keratocyte density was 20.2 ± 1.0 cells/CVE (n ...
Lots of ophthalmologic cosmetic surgeons are currently supplied the next evolution in LASIK surgical treatment, which they called Customized LASIK. The general treatment used to correct refractive mistakes coincides as is used for conventional LASIK treatments, with the enhancement of wavefront devices made use of to map the distinct surface area of the clients eye. The wavefront tool passes a narrow ray check out here of light through the systems as well as into the eye, measuring any optical distortions that result as the light leaves back out the eye. Any kind of abnormalities recognized are after that fed back right into the system so that the laser can automatically deal with for aberrations on the surface of the eye. Proponents of Personalized LASIK case that the use of wavefront modern technology allows cosmetic surgeons to deal with greater order aberrations that could add to low-light aesthetic disruptions such as halos as well as glare not corrected by conventional LASIK, eyeglasses, ...
Ocular local anesthetics currently used in routine clinical practice for corneal anesthesia are short acting and their ability to delay corneal healing makes them unsuitable for long-term use. In this study, we examined the effect of the site 1 sodiu
The cornea isnt a parcel of plastic. Conventional LASIK has become the most common kind of laser vision correction, and it has proven to be very effective for the correction of conventional vision errors. Blade Free LASIK (iLASIK) Imagine having the ability to perform most or all your activities of everyday life without the load…
Bilateral corneal anaesthesia and reduced sensation in the distribution of the ophthalmic division of the fifth cranial nerve associated with neuroparalytic keratitis and reduced vision is described in a 26-year-old woman with absence of the fallopian tubes, uterus, and upper vagina, a single pelvic kidney, and cervico-thoracic vertebral anomalies (the MURCS association). Other features of the disorder also present were short stature, facial asymmetry, micrognathia, and cleft palate. The neuroparalytic keratitis did not respond to tear substitutes and bilateral lateral tarsorrhaphies were eventually needed. This is the first report of congenital corneal anaesthesia in a case of MURCS. ...
The advanced surface procedure does not require the creation of a corneal flap. Instead, the superficial (epithelial) layer of the cornea is simply brushed aside and the excimer laser is applied to the surface to reshape the corneal tissue. This epithelial tissue usually regrows to cover the surface within a few days.. The biggest advantage of no-flap laser vision correction is the elimination of most of the flap-related risks. Most conventional LASIK is usually a safe and complication-free experience, but there are certain risks associated with a corneal flap. Chronic dryness, distorted vision from flap misplacement and early and late complications due to the structural weakness related to the corneal flap. With no-flap laser vision correction, these risks are virtually eliminated.. No-flap laser vision correction is a great option for many patients who do not qualify for conventional LASIK because they have thin corneas, or have had previous LASIK. For this reason, the military forces approved ...
De-epithelialised and de-endothelialised bovine corneal stromas with a hydration of 3.2 equilibrated at 154 mM NaCl and buffered at pH 7.4 had their optical density (400-750 nm) measured. Stromas equilibrated against 10, 20, 30, 50 or 100 mM NaCl made isotonic to 154 mM NaCl by supplementing with sorbitol were progressively more transparent as NaCl increased. Hypertonic equilibration against 300, 600 or 1000 mM NaCl resulted in a progressive loss of transparency compared with 154 mM NaCl. Light scattering as a function of wavelength fitted a l_3 function well for 10, 30, 50, 100 and 154 mM NaCl preparations between 450 and 650 nm, but not at higher wavelengths. However, hypertonic 300, 600 and 1000 mM NaCl preparations showed a l_2 dependence in the 450-750 nm range. Experiments with 154 mM NaCl and either 0 or 300 mM sorbitol suggested that the changes in light scattering in hypertonic preparations are unlikely to be caused by osmotic alterations to the stromal keratocytes. Psychophysical ...
RESULTS: The receptors for LXA4, ALX/Fpr-rs-2 and for RvE1, ChemR23 were each expressed by epithelium, stromal keratocytes, and infiltrated CD11b(+) cells in corneas. Compared to the vehicle-treated eye, ATLa-, RvD1-, and RvE1-treated eyes had reduced numbers of infiltrating neutrophils and macrophages and reduced mRNA expression levels of TNF-alpha, IL-1 alpha, IL-1 beta, VEGF-A, VEGF-C, and VEGFR2. Animals treated with these mediators had significantly suppressed suture-induced or IL-1 beta-induced hemangiogenesis (HA) but not lymphangiogenesis. Interestingly, only the application of ATLa significantly suppressed VEGF-A-induced HA ...
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
The mechanisms by which the eye maintains an immunosuppressive environment has been the subject of recent investigations. In this report we investigated the ability of resident ocular cells from the iris, choroid, and retina to inhibit lymphocyte responses in vitro. Our results demonstrate that sing …
second wave of migratory cells containing presumptive keratocytes invades the matrix, leading to formation of inner cornea and outer cornea. A unique cell mass (stroma attracting center) connects the two layers like the center pole of a tent ...
TY - JOUR. T1 - Lysophosphatidic acid activates TGFBIp expression in human corneal fibroblasts through a TGF-β1-dependent pathway. AU - Jeon, Eun Su. AU - Kim, Jae Ho. AU - Ryu, Hyunmi. AU - Kim, Eung Kweon. PY - 2012/6/1. Y1 - 2012/6/1. N2 - Granular corneal dystrophy type 2 (GCD2) is an autosomal dominant disease caused by a R124H point mutation in the transforming growth factor-β-induced gene (TGFBI). However, the cellular role of TGFBI and the regulatory mechanisms underlying corneal dystrophy pathogenesis are still poorly understood. Lysophosphatidic acid (LPA) refers to a small bioactive phospholipid mediator produced in various cell types, and binds G protein-coupled receptors to enhance numerous biological responses, including cell growth, inflammation, and differentiation. LPA levels are elevated in injured cornea and LPA is involved in proliferation and wound healing of cornea epithelial cells. Accumulating evidence has indicated a crucial role for LPA-induced expression of TGFBI ...
Bandage contact lenses (BCL) may help reduce pain and result in a less intense wound healing response following femtosecond laser in situ keratomileusis (FS-LASIK), according to a study in the Journal of Ophthalmology.. This prospective, randomized trial included 41 patients (82 eyes) with myopia and/or myopic astigmatism. After FS-LASIK surgery, patients were fitted with a BCL in one eye but not in the contralateral eye. The BCL was left in place overnight and removed the next morning. All eyes subsequently received standardized postoperative treatments. Investigators followed patients for six months and evaluated the subjects self-reported postoperative symptom scores for pain, photophobia, tearing and foreign-body sensation. At six months after surgery, researchers examined the corneal flap margin and adjacent regions and photographed them using slit-lamp biomicroscopy to subjectively evaluate the wound healing response.. The study reported postoperative pain and photophobia were milder in ...
Electron micrograph of keratocyte or fibroblast lamellipodial actin network after unprotected extraction. All examples demonstrate frequent branching...
Powstał z nudów więc z nudów pewnie będę na nim pisał. Tak naprawdę ten blog to taki wybieg w przyszłość - jak się w sobie zbiorę i zrobię do końca to co leży w szufladzie od jakiś 3 lat to prawdopodobnie blog będzie czymś w stylu zaplecza ...
Farmaceutyczne zbrodnie: HIV, pryszczyca, Hitler i testy na dzieciach. Koncerny farmaceutyczne są czyste i przejrzyste jak hipotetyczny tandem NSDAP z KPZR. Na wstępie omawiany już Baxter. Reakcja alergiczna na ich lek heparyn przyniosła od stycznia 2007 roku do kwietnia 2009 roku 103 zabitych… Stało się to powodem pozwu sądowego Joyce Ann Osteen złożonego 5 stycznia…
Tytu : Badania por wnawcze wp ywu k pieli wodnych z ozonem i k pieli bez ozonu skojarzonych z kinezyterapi na dolegliwo ci b lowe, wydolno czynno ciow staw w kolanowych oraz perfuzj mi ni ko czyn dolnych u os b z chorob zwyrodnieniow staw w kolanowych (doniesienie wst pne). ...
Corneal anaesthesia can be physiological or pathological. Corneal sensation decreases with age, and is lower in females, especially premenstrually. Contact lens wear, and infection by herpes zoster and simplex, oedema and surgery will also reduce sensation. Congenital causes of corneal anaesthesia include corneal dystrophy and Riley-Day syndrome, and congenital corneal anaesthesia without an associated syndrome, which is presumed to be due to hypoplasia of the ophthalmic division of the trigeminal nerve. Systemic disease such as diabetes, myotonic dystrophy, scleroderma, and vitamin deficiencies are important causes of corneal anaesthesia, which can often be overlooked. Forty five per cent of diabetic patients had a degree of corneal hypoaesthesia when examined in a study of 130 patients published by Osman et al.1 There is little or no relation between the age of a diabetic patient and the observed decrease in corneal sensitivity. However, corneal sensitivity thresholds do rise with increased ...
TY - JOUR. T1 - Analysis of the pattern of subcellular force generation by corneal fibroblasts after Rho activation. AU - Petroll, W. Matthew. AU - Ma, Lisha. AU - Ly, Linda. AU - Vishwanath, Mridula. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2008/1. Y1 - 2008/1. N2 - PURPOSE. To determine the structural and subcellular mechanical effects of Rho activation on corneal fibroblasts in three-dimensional collagen matrices. METHODS. Human corneal fibroblasts were plated at low density in 100-μm thick fibrillar collagen matrices and cultured for 1 or 2 days in serum-free media. Time-lapse imaging was then performed at 1- to 2-minute intervals with Nomarski differential interference contrast. After 1 hour, perfusion was switched to serum-free media containing 1 μmol/L lysophosphatidic acid (LPA). After an additional 30 to 60 minutes, the Rho kinase (ROCK) inhibitor Y-27632 was added to the perfusion media. Changes in cell structure and extracellular matrix deformation ...
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there was no statistically significant difference between both groups in �� Q value with Fs-LASIK inducing more oblate anterior corneal shape (P = 0.2124). Regarding corneal higher order aberrations, there was no statistically significant difference in the change in RMS of total corneal higher order aberration (P = 0.2) with more change of RMS of total aberrations in SMILE group .There was no statistically significant difference in RMS of coma aberration(P = 0.0589) ,however SMILE group showed more change in coma aberration. There is no statistically significant difference in RMS of spherical aberration (P = 0.0543 ...
The effects of vitamin A compounds on hyaluronic acid released from cultured rabbit corneal epithelial cells and keratocytes. - PubMed - NCBIA role...
Chambered cover glass /Glass bottom dishes / glass bottom plates at Cellvis (formerly In Vitro Scientific) for confocal microscopy studies. ideal for fluorescence microscopy.
WinSanTor is developing a proprietary first-in-class therapy to prevent and reverse nerve damage. Our lead compound, WST57, is a well-characterized off-patent compound with an established safety history that has been used for many years against an unrelated indication, this may allow a direct path to market and reducing development time and cost.. WST57 prevents, mitochondrial dysfunction, nerve fiber depletion and sensory loss in several distinct animal models of peripheral neuropathy.. We are strategically expanding WST57s indications to all neuropathies, such as chemo induced and HIV related. Additionally, WST57 is just one compound we are investigating in a family of promising compounds that we have exclusive world wide rights to.. ...
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Kim, B.Ki.; Mun, S.Joung.; Lee, D.Gyu.; Kim, J.Ryun.; Kim, H.Seung.; Chung, Y.Taek., 2015: Full-Thickness Astigmatic Keratotomy Combined With Small-Incision Lenticule Extraction to Treat High-Level and Mixed Astigmatism
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What is wavefront-optimized LASIK?. Wavefront-optimized LASIK is the type of laser treatment available on the Wavelight laser. This treatment is also based On the patients eye power and also takes into account corneal curvature and thickness, and applies laser energy in a unique fashion in the periphery of the cornea. This laser has been found to reduce the aforementioned complications such as glare, halos, and other nighttime visual aberrations that can occasionally occur with conventional treatments.. What is wavefront-guided LASIK?. Wave front-guided LASIK, also referred to as custom LASIK or wave front LASIK, is similar to conventional LASIK, except that in addition to treating a patients basic refractive error, specific alterations in a patients eye (high order aberrations) can also be treated. In wave front-guided LASIK, special mapping is performed prior to the surgery to identify any small irregularities in the patients optical system. When these irregularities are severe, they can ...
The iLASIK procedure is not without risk, but wearing contacts also comes with risks, particularly infection. To avoid infection, contacts require time and meticulous attention to proper cleaning. Still, infections with contacts do occur. Considering this risk, the constant attention they require and the lifetime costs for wearing them, maybe its time to leave your contacts behind and consider the iLASIK procedure.. iLASIK is an elective procedure with other alternatives including eyeglasses, photorefractive keratectomy (PRK), conventional LASIK, and other refractive surgeries. Only an eye care professional trained in laser vision correction can determine whether you are a suitable candidate. As with any surgical procedure, there are risks associated with the treatment. It is important to discuss the risks associated with the procedure and any questions you may have about the procedure with your doctor. Before deciding whether to have the procedure, you should ask your doctor for and carefully ...
V kategorii Bluetooth můžete vybírat z 69 produktů. Vyberte u nás pohodlně výpočetní a spotřební elektroniku a jejich prodejce. Račte vstoupit do obchodního katalogu Alave.cz ...
Laser correction for myopia and astigmatism using an excimer laser has been performed with success for several years.. During the last 2 years, a new method for correction of refractive errors has emerged. In the method (ReLex) a femtosecond laser is used to cut a lenticule in the corneal stroma that is subsequently removed. For simple myopia this method has proved effective and on par with excimer baser LASIK. However, it remains to be determined if ReLEx is as effective in the treatment of astigmatism.. In the study, patients with high astigmatism are treated with conventional LASIK for astigmatism in one eye and with ReLEx for astigmatism in the other. ...
Agnes S. Pascual is the author of these articles in the Journal of Visualized Experiments: Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing, Establishment of a Clinic-based Biorepository
W Polsce wskaźnik masy ciała ( body mass index - BMI) około 300 tysięcy osób wynosi powyżej 40 kg/m 2 , a ponad 1,5 miliona powyżej 35 kg/m 2 . 4 Problem otyłości w Polsce analizowany był w ramach projektów Pol-MONICA, NATPOL PLUS czy Wieloośrodkowego Ogólnopolskiego Badania Stanu Zdrowia Ludności (WOBASZ). W populacji polskiej w wieku pomiędzy 20. a 74. rokiem życia średni BMI poniżej 25 kg/m 2 stwierdzono jedynie u 47% badanych, u 34% odnotowano nadwagę, a u 19% otyłość. 5-7 Według danych GUS z 2009 roku problem ten dotyczył ponad 61% mężczyzn (45% miało nadwagę, 17% otyłość) i prawie 45% kobiet (30% miało nadwagę, a 15% otyłość). 8 Otyłość w sposób szczególny zagraża najmłodszym. Nadmierna masa ciała w dzieciństwie i w okresie dojrzewania prowadzi do otyłości u dorosłych. W Europie nadwaga jest najpowszechniej występującym problemem zdrowotnym wieku dziecięcego. Według danych z 2013 roku na świecie u niemal 42 milionów dzieci po 5. roku ...
Tytu : Wp yw zabiegu izolacji uj y p ucnych metod krioablacji balonowej na cz sto nawrot w migotania przedsionk w u os b z nieprawid ow mas cia a - obserwacje wst pne. ...
Martwicze zapalenie jelit (NEC) jest stanem nagłym u noworodków, związanym z zaburzeniami funkcjonowania przewodu pokarmowego. Zmiany histologiczne polegają na występowaniu mnogich ognisk odcinkowej martwicy ściany jelita, głównie końcowego odcinka jelita krętego oraz okrężnicy wstępującej, aczkolwiek często dotyczą całego jelita.
Keratocytes are flattened cells found dispersed within the corneal stroma. The primary role of this sparse population of cells ... It has also been found that the presence of secreted defensins secreted by corneal keratocytes is correlated with cases of ... Corneal nerves serve as a form of defense by detecting the presence of foreign bodies on the corneal surface. This leads to ... Other components of the ocular innate immune system include tears, epithelial cells, keratocytes, corneal nerves, the ...
It is produced by corneal keratocytes and is thought to play a role of a dynamic buffer of corneal hydration. In a rare ... Whereas corneal KSI is composed of a number of domains showing variable degrees of sulphation the longest of which may be 8-32 ... Macular dystrophy, corneal, 1 - OMIM Lauder RM, Huckerby TN, Nieduszynski IA (1997). "The structure of the keratan sulphate ... Tai GH, Huckerby TN, Nieduszynski IA (1996). "Multiple non-reducing chain termini isolated from bovine corneal keratan sulfates ...
The lamellæ are produced by keratocytes (corneal connective tissue cells), which occupy about 10% of the substantia propria. ... Macular corneal dystrophy, associated with the loss of keratan sulfate Oyster, CW (1999). "8". The human eye: structure and ... During development of the embryo, the corneal stroma is derived from the neural crest (a source of mesenchyme in the head and ... Branch MJ, Hashmani K, Dhillon P, Jones DR, Dua HS, Hopkinson A (Aug 3, 2012). "Mesenchymal stem cells in the human corneal ...
In the corneal stroma, keratocytes within the wounded area undergo apoptosis, leaving the stroma devoid of cells that must be ... Keratocytes surrounding the wounded area proliferate and become fibroblasts that migrate to fill the wounded area. This creates ... For example, when corneal integrity is compromised, epithelial cells quickly cover the damaged area by proliferation and ... "Role of Thrombospondin-1 in Repair of Penetrating Corneal Wounds". Investigative Ophthalmology & Visual Science. 54 (9): 6262- ...
Rabinowitz, YS; McDonnell, PJ (1989). "Computer-assisted corneal topography in keratoconus". Refractive & Corneal Surgery. 5 (6 ... The visible changes in the basal epithelial cell and anterior and posterior keratocytes linked with keratoconus were not ... custom topography-guided transepithelial PRK combined with corneal collagen cross-linking, or corneal transplant. When cross- ... "Post-LASIK ectasia treated with intrastromal corneal ring segments and corneal crosslinking". Digital Journal of Ophthalmology ...
The number of corneal keratocytes in the epithelial stroma has not been found to change with long-term contact lens wear. ... Increased corneal curvature is yet another change known to arise from long-term contact lens wear; this increase in corneal ... Increases in corneal curvature are thought to be caused by corneal thinning-induced ectasia. Two explanations have been ... Long-term contact lens use can lead to alterations in corneal thickness, stromal thickness, curvature, corneal sensitivity, ...
Corneal pachymetry Corneal reflex Corneal tattooing Corneal topography Eye disease Keratometry List of keratins expressed in ... consisting of regularly arranged collagen fibers along with sparsely distributed interconnected keratocytes, which are the ... For corneal epithelial diseases such as Stevens Johnson Syndrome, persistent corneal ulcer etc., the autologous contralateral ( ... The most common corneal disorders are the following: Corneal abrasion - a medical condition involving the loss of the surface ...
Satellite glial cell Chromaffin cell Glomus cell Melanocyte Nevus cell Merkel cell Odontoblast Cementoblast Corneal keratocyte ...
His research has focused on corneal cellular responses to injury and surgery, with his most-cited work being about keratocyte ... He holds two United States patents related to corneal healing. He previously served as a professor and chair of the Department ... CS1 maint: discouraged parameter (link) "Methods and treatments for corneal healing with growth factors". Free Patents Online. ... CS1 maint: discouraged parameter (link) "Methods and treatments for corneal healing with hepatocyte and keratinocyte growth ...
Play media Corneal keratocytes (corneal fibroblasts) are specialized fibroblasts residing in the stroma. This corneal layer, ... MACULAR DYSTROPHY, CORNEAL, 1; MCDC1 - OMIM. Patel S, McLaren J, Hodge D, Bourne W (February 2001). "Normal human keratocyte ... Quiescent keratocytes synthesize the so-called crystallins, known primarily for their role in the lens. Corneal crystallins, ... Keratocytes may play a role in different corneal disorders. According to comparative research, their functions drastically ...
... corneal transplantation) or corneal transplant. The Boston KPro is a proven primary treatment option for repeat graft failure, ... The holes allow the aqueous humour fluids of the eye to provide nutrients to the donor graft stroma and keratocytes. The device ... corneal transplantation). The Boston KPro is a treatment option for corneal disorders not amenable to standard penetrating ... During implantation of the device, the device is assembled with a donor corneal graft positioned between the front and back ...
Corneal keratocyte(英语:Corneal keratocyte). *Ocular immune system(英语:Ocular immune system) ... Daxer A, Misof K, Grabner B, Ettl A, Fratzl P. "Collagen fibrils in the human corneal stroma: structure and aging." Invest ... Daxer A, Fratzl P. "Collagen fibril orientation in the human corneal stroma and its implication in keratoconus." Invest ... Facts About The Cornea and Corneal Disease National Eye Institute (NEI). *Keratoconus (Irregular Astigmatism), Patient Support ...
Long-term wear of rigid contacts is associated with decreased corneal keratocyte density[63] and increased number of epithelial ... corneal abrasions and erosion, keratitis, corneal edema, descemetocele, corneal ectasis, Mooren's ulcer, anterior corneal ... Wearing lenses designed for daily wear overnight has an increased risk for corneal infections, corneal ulcers and corneal ... "The Corneal Lens", The Optician, 2 September 1949, pp. 141-144. *^ "Corneal Contact Lenses", The Optician, 9 September 1949, p ...
Keratocyte phenotype mediates proteoglycan structure: a role for fibroblasts in corneal fibrosis ,journal=[[J. Biol. Chem.]] , ... Nun trastorno progresivo raro chamado [[distrofia corneal macular]], a síntese de queratán sulfato está ausente ou é anormal , ... title = Distribution of proteoglycans antigenically related to corneal keratan sulfate proteoglycan , journal = J. Biol. Chem ... ref name="OMIM_MCDC",[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=217800 Macular dystrophy, corneal, 1] - OMIM,/ref,. ...
After corneal injury, keratocytes become activated and transform into repair phenotypes-corneal fibroblasts or myofibroblasts, ... and in keratocytes in normal human corneal tissue sections. Thy-1 mRNA and protein were detectable in cultured human corneal ... Cultured human corneal fibroblasts and myofibroblasts both labelled for Thy-1, but keratocytes in the stroma of normal human ... Human corneal keratocytes were isolated by collagenase digestion. The cells were either processed immediately (i.e. freshly ...
A: Freshly isolated primary keratocytes were cultured in polyHEMA-coated dishes either in Advanced-DMEM (ADV, d ... Purpose: Keratocytes, mesenchymal cells populating the corneal stroma, secrete the unique transparent connective tissue of the ... Purpose: Keratocytes, mesenchymal cells populating the corneal stroma, secrete the unique transparent connective tissue of the ... f2: All keratocytes participate in sphere formation. A: Freshly isolated primary keratocytes were cultured in polyHEMA-coated ...
Play media Corneal keratocytes (corneal fibroblasts) are specialized fibroblasts residing in the stroma. This corneal layer, ... MACULAR DYSTROPHY, CORNEAL, 1; MCDC1 - OMIM. Patel S, McLaren J, Hodge D, Bourne W (February 2001). "Normal human keratocyte ... Quiescent keratocytes synthesize the so-called crystallins, known primarily for their role in the lens. Corneal crystallins, ... Keratocytes may play a role in different corneal disorders. According to comparative research, their functions drastically ...
Keratocytes were the major cells expressing MMP13 in corneal stromas after alkali burns. Through MMP13 expression, keratocytes ... Keratocytes create stromal spaces to promote corneal neovascularization via MMP13 expression.. [Jing Ma, Dan Zhou, Meihua Fan, ... Keratocytes could directly degrade type I collagen and create stromal spaces, promoting corneal neovascularization through ... To investigate the exact mechanism by which keratocytes promote corneal neovascularization. The expression of matrix ...
... the corneal stroma where they acquired a keratocyte-like morphology and an expression profile similar to corneal keratocytes in ... the embryonic precursor to keratocytes, and then cultured on cadaveric corneal tissue to promote keratocyte differentiation. ... These results indicate that hiPSCs can be used to generate corneal keratocytes in vitro and lay the foundation for using these ... The ability to generate corneal keratocytes in vitro may enable a cell-based therapy to treat patients with keratoconus. Human ...
Role of Keratocyte Loss on Corneal Wound Repair after LASIK. In Vivo Gene Delivery and Visualization of Corneal Stromal Cells ... The absence of endogenous NT in corneal keratocytes suggests that NT can be present in corneal nerves or released by nearby ... Keratocyte proliferation studied by MTS assay. Human corneal keratocytes were cultured with various concentrations of JMV449 ( ... Keratocyte proliferation studied by MTS assay. Human corneal keratocytes were cultured with various concentrations of JMV449 ( ...
Rabbit corneal keratocytes were plated within standard collagen matrices (2.5 mg/mL) or compressed collagen matrices (~100 mg/ ... biophysical properties modulate corneal keratocyte phenotypes in response to specific wound healing cytokines and Rho GTPases. ... Overall, keratocyte growth factor responses appear to be regulated by both the interplay between Rho and Rac signaling, and the ... Keratocytes within compressed collagen (which has high stiffness and low porosity) maintained a dendritic phenotype following ...
Method for Detecting Corneal Endothelial Cell Identity From Contaminating Keratocytes and Epithelial Cells in Co-Culture ... Method for Detecting Corneal Endothelial Cell Identity From Contaminating Keratocytes and Epithelial Cells in Co-Culture ... Method for Detecting Corneal Endothelial Cell Identity From Contaminating Keratocytes and Epithelial Cells in Co-Culture. ... SMA was expressed in cultured keratocytes but not in whole stromal samples or HCEC. ANGPTL6 and 7 were both expressed in ...
Corneal keratocytes from six New Zealand White Rabbits were isolated and cultured until Passage 1. The proliferative effects of ... This study aimed to investigate the effects of EBN on corneal keratocytes proliferative capacity and phenotypical changes. ... Both phase contrast micrographs and gene expression analysis confirmed the corneal keratocytes retained their phenotypes with ... EBN on corneal keratocytes were determined by MTT assay in serum-containing medium (FDS) and serum-free medium (FD). ...
Automated detection and cell density assessment of keratocytes in the human corneal stroma from ultrahigh resolution optical ... Automated detection and cell density assessment of keratocytes in the human corneal stroma from ultrahigh resolution optical ... images of the human corneal stroma is presented. The corneal OCT data is first processed using a state-of-the-art despeckling ... Keratocytes are fibroblast-like cells that maintain the optical clarity and the overall health of the cornea. The ability to ...
... on the differentiation of rabbit corneal keratocytes. Keratocytes grown in 10% FBS differentiated into myofibroblasts by ... Thus, exosomal miR-19a derived from the ADSCs suppresses FBS-induced differentiation of rabbit corneal keratocytes into ... HIPK2 enhanced p53 and Smad3 pathways in FBS-induced keratocytes. Keratocytes grown in 10% FBS also showed increased levels of ... Keratocytes treated with 10% FBS and ADSCs-Exo-miR-19a-agomir or ADSCs-Exo-NC-antagomir showed significantly lower levels of ...
Keratocytes are flattened cells found dispersed within the corneal stroma. The primary role of this sparse population of cells ... It has also been found that the presence of secreted defensins secreted by corneal keratocytes is correlated with cases of ... Corneal nerves serve as a form of defense by detecting the presence of foreign bodies on the corneal surface. This leads to ... Other components of the ocular innate immune system include tears, epithelial cells, keratocytes, corneal nerves, the ...
A correlation was attempted between the keratocyte loss, histologic, microbiologic and clinical features. The corneal tissues ... In a study of corneal buttons obtained from patients of corneal ulcer undergoing therapeutic keratoplasty, we observed loss of ... loss of keratocytes in the surrounding stroma (posterior or peripheral). TUNEL positivity was evaluated in the corneal sections ... We report apoptotic cell death of keratocytes in the corneal stroma in infectious keratitis, a phenomenon independent of type ...
Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts. / Karring, Henrik ; ... Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts. I: Investigative ... title = "Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts", ... T1 - Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts ...
Effects of edible birds nest EBN on cultured rabbit corneal keratocytes. . Biblioteca virtual para leer y descargar libros, ... Effects of edible birds nest EBN on cultured rabbit corneal keratocytes - Descarga este documento en PDF. Documentación en PDF ... This study aimed to investigate the effects of EBN on corneal keratocytes proliferative capacity and phenotypical changes.. ... Both phase contrast micrographs and gene expression analysis confirmed the corneal keratocytes retained their phenotypes with ...
Three months later, keratocyte density was increased in all anteroposterior stromal regions, suggesting that PRK affects ... Keratocyte density was increased in the anterior stroma immediately after PRK in humans. ... Keratocyte density in vivo after photorefractive keratectomy in humans Trans Am Ophthalmol Soc. 1999;97:221-36; discussion 236- ... Conclusions: Keratocyte density was increased in the anterior stroma immediately after PRK in humans. Three months later, ...
Human corneal keratocytes (HCK) are essential for maintaining corneal structure and transparency. This study shows that the ... Blocking TRPV1 activation on keratocytes may therefore be a viable approach to suppress corneal opacification in a clinical ... transient receptor potential vanilloid type 1 activity and myofibroblast transdifferentiation in human corneal keratocytes ...
Corneal keratocytes from New Zealand white rabbits were cultured in basal medium (BM) and serum enriched medium (BMS). Serial ... Gelam honey at a concentration of 0.0015 % promotes ex vivo corneal keratocytes proliferation while retaining desirable ... MTT assay was performed on corneal keratocytes in both media to ascertain the optimal dose of GH that produced maximum ... The results serve as a basis for the development of Gelam honey as a potential natural product in promoting corneal wound ...
Human corneal stromal keratocytes cultured with F-AME or C-AME showed similar morphology and viability, while cell ... can correct early corneal haze in an animal model. Clinical application of cultivated keratocytes is limited by infectious ... Primary human keratocytes from 4 donor corneas were cultured in media supplemented with fresh AME (F-AME) or cryopreserved AME ... It remains unclear if AME from cryopreserved versus fresh human amnion can support human keratocyte propagation, and which ...
... primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate ... primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate ... primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate ... primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate ...
Normal human keratocytes were purchased from ScienCell Research Lab (Carlsbad, CA). Schnyder corneal dystrophy and normal human ... Cell lines were established from patients including keratocytes obtained after corneal transplant surgery and lymphoblastoid ... The resultant bilateral corneal opacification is progressive. Approximately 50% of SCD patients have corneal crystalline ... Notation was made as to the presence of central corneal opacity, mid peripheral haze, arcus lipoides and corneal crystalline ...
Corneal Keratocyte Apoptosis Following Topical Intraoperative Mitomycin C in Rabbits Shu-Wen Chang, MD ... The Effect of Tear Film on Higher Order Corrections Applied to the Corneal Surface During Wavefront-guided Refractive Surgery ... VHF Digital Ultrasound Three-Dimensional Scanning in the Diagnosis of Myopic Regression After Corneal Refractive Surgery Dan Z ...
In vitro effects of dexamethasone on human corneal keratocytes.. Bourcier T, Borderie V, Forgez P, Lombet A, Rostène W, Laroche ... Expression of neurotensin receptors in human corneal keratocytes.. Bourcier T, Rondeau N, Paquet S, Forgez P, Lombet A, Pouzaud ... Regulation of human corneal epithelial cell proliferation and apoptosis by dexamethasone.. Bourcier T, Forgez P, Borderie V, ...
keywords = "Corneal confocal microscopy, Corneal epithelial basal cells, Corneal nerves, Dry eye, Keratocytes, Type 1 diabetes ... There was no correlation between DES and TBUT or corneal nerve keratocyte and CEBC morphology. CONCLUSIONS. DES and TBUT are ... There was no correlation between DES and TBUT or corneal nerve keratocyte and CEBC morphology. CONCLUSIONS. DES and TBUT are ... There was no correlation between DES and TBUT or corneal nerve keratocyte and CEBC morphology. CONCLUSIONS. DES and TBUT are ...
Keratocytes Corneal Stroma * Adult Corneal Superbasal Epithelial Cells Corneal Epithelium * Adult Corneal Basal Epithelial ... Fetal Corneal Superficial Epithelial Cells Corneal Epithelium * ... Corneal Epithelium * Adult Corneal Superbasal Epithelial Cells ... Adult Corneal Superficial Epithelial Cells Corneal Epithelium * Fetal Corneal Superficial Epithelial Cells ... Adult Corneal Superficial Epithelial Cells Corneal Epithelium * ... Corneal Epithelium * Adult Corneal Basal Epithelial Cells ...
Keratocytes Corneal Stroma *span" data-trigger="SectionLoaded"> Kidney (Urinary System) * Type B cells Renal Collecting Duct ...
Chick corneal morphogenesis: A new role for keratocytes. Investigative Ophthalmology & Visual Science 2017; 58 (8), 1998. ... Immune Cells on the Corneal Endothelium of an Allogeneic Corneal Transplantation Rabbit Model. Investigative Ophthalmology & ... Chemical composition and sulfur speciation in bulk tissue by x-ray spectroscopy and x-ray microscopy: Corneal development ... X-ray absorption near-edge structure (XANES) spectroscopy identifies differential sulfur speciation in corneal tissue. ...
Chick corneal morphogenesis: A new role for keratocytes. Investigative Ophthalmology & Visual Science 2017; 58 (8), 1998. ... Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model. Investigative Ophthalmology & ... Immune cells on the corneal endothelium of an allogeneic corneal transplantation rabbit model. Investigative Ophthalmology & ... Immune Cells on the Corneal Endothelium of an Allogeneic Corneal Transplantation Rabbit Model. Investigative Ophthalmology & ...
Keratocytes are the resident cells of the corneal stroma and their function isto produce extracellular matrix components and to ... Open this publication in new window or tab ,,Neuropeptides and neurotransmitters in keratocytes: importance in corneal wound ... 1. Neuropeptides and neurotransmitters in keratocytes: importance in corneal wound healing processes. ... The process of corneal wound healing is complex. Shortly, keratocytesadjacent to the corneal wound undergo apoptosis and ...
PROGRAMMED KERATOCYTE DEATH AFTER CORNEAL EPITHELIAL INJURY: IMPLICATIONS FOR WOUND HEALING AFTER CORNEAL LASER SURGERY. Wilson ... QUALITY OF CORNEAL DONOR TISSUE IN THE GREATER THAN 75 YEAR AGE GROUP. Probst, Louis E.; Halfaker, Bridget A.; Holland, Edward ... CORNEAL EXTRACELLULAR MATRIX CHANGES AFTER MULTIPLE REFRACTIVE PROCEDURES. Maguen, E.; Burgeson, R E; Butkowski, R J; More ... TRANSPLANTATION OF CORNEAL TISSUE FROM DONORS WITH DISEASES OF THE CENTRAL NERVOUS SYSTEM. Hogan, R Nick; Cavanagh, H Dwight ...
Ocular local anesthetics currently used in routine clinical practice for corneal anesthesia are short acting and their ability ... to delay corneal healing makes them unsuitable for long-term use. In this study, we examined the effect of the site 1 sodiu ... Corneal Keratocytes / drug effects. Epithelium, Corneal / drug effects. Humans. Male. Propoxycaine / pharmacology*. Quaternary ... The effect of test compounds on the rate of corneal epithelialization was studied in vivo after corneal debridement.. RESULTS: ...
  • Keratocytes, mesenchymal cells populating the corneal stroma, secrete the unique transparent connective tissue of the cornea as well as opaque scar tissue after injury. (nih.gov)
  • Primary keratocytes from collagenase digestion of bovine corneas were cultured on tissue-culture plastic or on poly (2-hydroxyethylmethacrylate)(polyHEMA)-coated, non-adhesive surfaces. (nih.gov)
  • Keratocyte phenotype is enhanced in the absence of attachment to the substratum. (nih.gov)
  • Previous studies identified factors mediating keratocyte phenotype in vitro, particularly the expression of the keratan sulfate proteoglycans, which are essential for vision. (nih.gov)
  • Whereas earlier work emphasized effects of cytokines, the current study examines the effects of substratum attachment on keratocyte phenotype. (nih.gov)
  • Similarly, the platelet derived growth factor, a cytokine initiating the fibroblastic phenotype in attached keratocytes, had a limited effect on the spheroid-associated keratocytes. (nih.gov)
  • For primary keratocytes, the alteration of the cellular environment in terms of cell-cell and cell-matrix interactions mediates and can override signals from soluble cytokines in influencing matrix expression and also in adopting other aspects of the fibroblastic and myofibroblastic phenotypes found in healing wounds. (nih.gov)
  • Spheroid keratocytes secrete more keratan sulfate and keratocan than attached cells in the same culture medium. (nih.gov)
  • Matrix metalloproteinase 13 was predominantly expressed by epithelial cells in normal rat corneas, but it was expressed by cells in corneal stromas after alkali burns. (sigmaaldrich.com)
  • found that contact with corneal fibroblasts stimulated corneal epithelial cells to produce the matrix metalloprotease MMP9. (sciencemag.org)
  • 3 It has been proposed that apoptosis, initiated by injury of corneal epithelial cells, induces the disappearance of anterior keratocytes. (bmj.com)
  • In certain embodiments, the scaffold can be impregnated with one or more keratocytes and a surface of the convex curvature of the scaffold can be covered with one or more layers of epithelial cells. (patents.com)
  • and (d) a motor coupled to the fabricated eyelid, wherein the scaffold is impregnated with one or more keratocytes and a surface of the convex curvature of the scaffold is covered with one or more layers of epithelial cells. (patents.com)
  • 3. The biomimetic eye model of claim 1, wherein the one or more layers of epithelial cells comprises one or more layers of corneal epithelial cells and one or more layers of conjunctival cells in a concentric circular pattern. (patents.com)
  • 4. The biomimetic eye model of claim 3, wherein the one or more layers of the corneal epithelial cells are present within an inner circle of the concentric circular pattern. (patents.com)
  • These experiments will entail the characterization of mouse corneal epithelial cells and mouse corneal keratocytes (a specialized fibroblast) using mammalian cell culture techniques. (csbsju.edu)
  • Corneal epithelial cells require HS for maintaining corneal homeostasis, and the loss of epithelial HS leads to both impaired wound healing and impaired corneal stratification. (sigmaaldrich.com)
  • In addition, as a general finding in all species tested, there was an apparent increase noted in P63 expression in basal corneal epithelial cells in regions that had increased cellular layers (limbus in humans and rats and the paracentral corneal region in the mouse). (ebscohost.com)
  • Exosomes secreted by mouse corneal epithelial cells were found to fuse to keratocytes in vitro and to induce myofibroblast transformation. (harvard.edu)
  • Our results indicate that epithelial cell-derived exosomes mediate communication between corneal epithelial cells and corneal keratocytes as well as vascular endothelial cells. (harvard.edu)
  • Other neighbouring keratocytes, when acted upon by the same molecules, become active, proliferate and start synthesizing matrix metalloproteinases that cause tissue remodeling. (wikipedia.org)
  • X-ray absorption near-edge structure (XANES) spectroscopy identifies differential sulfur speciation in corneal tissue. (cardiff.ac.uk)
  • Chemical composition and sulfur speciation in bulk tissue by x-ray spectroscopy and x-ray microscopy: Corneal development during embryogenesis. (cardiff.ac.uk)
  • 2017. Template curvature influences cell alignment to create improved human corneal tissue equivalents . (cardiff.ac.uk)
  • Refractive surgery, as it is known today, was not realized until 1966 when Pureskin first appreciated its potential with the demonstration that refractive changes could be made by removing central tissue underneath a corneal flap. (medscape.com)
  • The lamellæ are produced by keratocytes (corneal connective tissue cells), which occupy about 10% of the substantia propria. (wikipedia.org)
  • These techniques involve the use of a pulsed laser photoablation apparatus to ablate very thin layers of corneal tissue with greater precision than can typically be achieved with mechanical means. (google.com)
  • My research team seeks to engineer functional replacement and temporary 'bridge' tissues using a modular approach while also developing model systems to study physiological and pathophysiological corneal tissue formation. (ncl.ac.uk)
  • LASIK applies the energy underneath a flap of corneal tissue. (usaeyes.org)
  • Surface ablation techniques like PRK, and its cousins LASEK and Epi-LASIK, do not use a flap of corneal tissue, but have a higher rate of corneal haze than LASIK. (usaeyes.org)
  • Corneal biomechanics is a branch of science that studies deformation and equilibrium of corneal tissue under the application of any force [ 1 ]. (hindawi.com)
  • Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. (nih.gov)
  • The purpose of this experimental study is to evaluate the immediate effect of the CXL treatment on the biomechanical properties and histological microstructure of healthy human corneal tissue. (escrs.org)
  • With this purpose, in vitro mechanical tests and histological observation of cross-linked and untreated human corneal tissue were performed. (escrs.org)
  • Mechanical tensile test and histological analysis were performed in de-epithelialized healthy human corneal tissue treated with riboflavin solution (0.01% riboflavin-5-phosphate and 20% dextranT-500) - 30 min UVA irradiation (treated sample TS). (escrs.org)
  • A similar study was performed to untreated de-epithelialized healthy human corneal tissue (control sample, CS). (escrs.org)
  • The treated corneal tissue (TS) resulted 1.8, 1.6, 1.7 and 1.5 fold stiffer than the untreated sample (CS) at 6, 8, 10 and 12% stretch, respectively. (escrs.org)
  • The UVA-induced CXL has an immediate biomechanical and histological effect over the treated healthy human corneal tissue. (escrs.org)
  • Stiffening of the tissue, keratocyte apoptosis and stromal compacting were observed. (escrs.org)
  • The findings, published online today in STEM CELLS Translational Medicine , indicate they also could become a new source of corneal transplant tissue made from the patient's own cells. (upmc.com)
  • Regeneration and fibrosis of corneal tissues Simirskii, V. 2014-09-12 00:00:00 In this review, the features of the regeneration of corneal tissue and its disorders leading to the development of fibrosis are considered. (deepdyve.com)
  • The main steps of regeneration of corneal tissues and their disorders that lead to outstripping proliferation of myofibroblasts and secretion of extracellular matrix in the wound area and eventually cause the formation of connective tissue scar and corneal opacity are considered. (deepdyve.com)
  • Particular attention is given to the successes of translational medicine in the treatment of corneal tissue fibrosis. (deepdyve.com)
  • In 1935, V. P. Filatov was the first to report successful storage of whole eyeglobes at 4°C. The opportunity to store corneal tissue is crucial for extensive use of keratoplasty as treatment for corneal blindness. (uio.no)
  • Finally, recent advantages in preservation of cultured corneal/limbal epithelia may increase the availability of this tissue and therefore increase treatment of limbal stem cell deficiency. (uio.no)
  • The results also illustrate the structural necessity of fibre inclination in stabilizing the corneal refractive surface with respect to changes in tissue hydration and IOP. (royalsocietypublishing.org)
  • Burratto's original work involved performing a corrective excimer laser ablation on the back of a resected disc of corneal tissue. (medscape.com)
  • Donor corneal graft tissue was obtained from the paracentral region or the anatomical limbal region of murine corneas. (ebscohost.com)
  • The current and projected shortage of transplantable human donor corneas has prompted the development of long-term alternatives to human donor tissue for corneal replacement. (diva-portal.org)
  • By the moment of eye opening after birth the proliferation of keratocytes is all but finished and most of them are in the quiescent state. (wikipedia.org)
  • This could be a novel breakthrough as both cell proliferation and functional maintenance are important during corneal wound healing. (biomedcentral.com)
  • Cell viability, proliferation and keratocyte marker expression were examined by confocal immunofluorescence and flow cytometry. (biomedcentral.com)
  • Shortly, keratocytesadjacent to the corneal wound undergo apoptosis and remaining cells start the process of proliferation andmigration in order to close the wound. (diva-portal.org)
  • Moreover,it is hypothesized that the non-neuronal SP and ACh produced by injured keratocytes participate in cornealwound healing by enhancing keratocyte migration and proliferation, and/or by decreasing keratocyteapoptosis. (diva-portal.org)
  • The optimal dose of AH in the basal medium (FD) and medium containing serum (FDS) for keratocytes proliferation was identified using MTT assay. (blogspot.com)
  • Acetylcholine enhances keratocyte proliferation through muscarinic receptor activation. (umu.se)
  • genetic disruption of its synthesis leads to the macular corneal dystrophy. (wikipedia.org)
  • According to comparative research, their functions drastically diverge from the norm in keratoconus, the most frequent form of corneal dystrophy. (wikipedia.org)
  • Mutations in a novel gene, UBIAD1 , were recently found to cause the autosomal dominant eye disease Schnyder corneal dystrophy (SCD). (pubmedcentralcanada.ca)
  • To study the clinical, histological, in vivo confocal microscopic, and molecular profile in a family with gelatinous drop-like corneal dystrophy (GDLD) from north India. (molvis.org)
  • Recurrent macular corneal dystrophy following penetrating keratoplasty. (harvard.edu)
  • Researchers have discovered that people who carry a mutated form of the transcription factor 4 gene (TCF4) have as much as a 30-fold increased risk of developing Fuchs's corneal dystrophy. (bionews.org.uk)
  • Through MMP13 expression, keratocytes directly degraded collagen type I to create stromal spaces, which were convenient for newly formed blood vessels to grow into. (sigmaaldrich.com)
  • Expression of MMP13 and collagen type I degradation via keratocytes were induced by VEGFc through VEGFr3 and inhibited by antibodies for VEGFc and VEGFr3. (sigmaaldrich.com)
  • Keratocytes could directly degrade type I collagen and create stromal spaces, promoting corneal neovascularization through VEGFc/VEGFr3-induced MMP13 expression. (sigmaaldrich.com)
  • Rabbit corneal keratocytes were plated within standard collagen matrices (2.5 mg/mL) or compressed collagen matrices (~100 mg/mL) and cultured in serum-free media, PDGF BB, IGF, FGF2 or TGFβ1, with or without the Rac1 inhibitor NSC23766 and/or the Rho kinase inhibitor Y-27632. (mdpi.com)
  • Keratocytes within standard collagen matrices (which are highly compliant) maintained a dendritic phenotype following culture in serum-free media, PDGF, IGF and FGF, but developed stress fibers in TGFβ1. (mdpi.com)
  • Keratocytes within compressed collagen (which has high stiffness and low porosity) maintained a dendritic phenotype following culture in serum-free media, PDGF and IGF, but developed stress fibers in both FGF and TGFβ1. (mdpi.com)
  • Keratocytes phenotypical changes were morphologically assessed and gene expression of aldehyde dehydrogenase (ALDH), collagen type 1 and lumican were determined through RT-PCR. (biomedcentral.com)
  • 2018. Cell regulation of collagen fibril macrostructure during corneal morphogenesis . (cardiff.ac.uk)
  • Cloned corneal SP cells under chondrogenic conditions produced matrix staining with toluidine blue and expressed cartilage-specific markers: collagen II, cartilage oligomatrix protein, and aggrecan. (mendeley.com)
  • Corneal bioprinting utilizing collagen-based bioinks and primary human keratocytes. (ukaachen.de)
  • Keratocytes are induced to produce collagen type II: A new strategy for in vivo corneal matrix regeneration. (nih.gov)
  • These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. (nih.gov)
  • Lumican regulates collagen fibril structure such that gene targeted mice deficient in lumican manifest Ehlers Danlos Syndrome-like skin fragility, tendon weaknesses and corneal opacity. (hopkinsmedicine.org)
  • Composite core-and-skirt collagen hydrogels with differential degradation for corneal therapeutic applications. (diva-portal.org)
  • This corneal layer, representing about 85-90% of corneal thickness, is built up from highly regular collagenous lamellae and extracellular matrix components. (wikipedia.org)
  • The goal of this study was to investigate how alterations in extracellular matrix (ECM) biophysical properties modulate corneal keratocyte phenotypes in response to specific wound healing cytokines and Rho GTPases. (mdpi.com)
  • Human corneal stromal keratocytes propagated in culture media supplemented with human amnion extract (AME) can correct early corneal haze in an animal model. (biomedcentral.com)
  • Our group has previously reported on the use of intrastromal injection of CSKs to arrest corneal haze development and restore corneal clarity in a rat model of early corneal opacities [ 12 ]. (biomedcentral.com)
  • This invention relates to the compositions and methods for the treatment of corneal haze. (google.com)
  • The compositions and methods are particularly directed to the use of GAG synthesis inhibitors in the treatment of corneal haze. (google.com)
  • 1. A method for the treatment of corneal haze which comprises administering to a human patient prior to, during or after corneal laser surgery, or combinations thereof, one or more compositions comprising an effective amount of one or more GAG synthesis inhibitor(s) and a pharmaceutically acceptable vehicle. (google.com)
  • Photoablation, however, is followed by a more intense inflammatory response and a greater increase in backscattering of light, commonly referred to as "corneal haze," that is associated with increased keratocyte activation and myofibroblast transformation. (google.com)
  • The magnitude of corneal wound repair and the development and duration of corneal haze appears to increase proportionally with in creasing stromal photoablation depth (ice. (google.com)
  • Mitomycin C is a medication that changes keratocyte activity and also reduces the probability of late-onset corneal haze in higher myopes and surface ablation correction. (usaeyes.org)
  • The LASIK flap virtually eliminates the need for application of Mitomycin C to reduce the probability of corneal haze. (usaeyes.org)
  • Equally, the flap reduces the probability of late-onset corneal haze by using a process that limits or reduces the cornea's healing response in a positive and constructive way. (usaeyes.org)
  • If you have PRK alone, there is a relatively high probability of having corneal haze. (usaeyes.org)
  • This will help reduce the probability of corneal haze with all procedures. (usaeyes.org)
  • 1 Since then, MMC usage has expanded significantly within the field of refractive surgery, especially during photorefractive keratectomy (PRK), for prevention of recurrence of corneal haze and as prophylaxis to prevent initial haze development. (pubmedcentralcanada.ca)
  • A corneal haze was first noted at 45 degrees C X 45 minutes X 1 day follow-up. (mendeley.com)
  • Since its introduction, LASIK has been associated with various complications, specifically when performed on eyes with decreased corneal thickness, irregular astigmatism, dryness, preexisting ocular surface disease, or glaucoma, to the point where several of these entities have become relative contraindications to performing LASIK. (medscape.com)
  • The aim of this study was to compare the pre- and posttreatment corneal densitometry and corneal thickness value of keratoconus (KCN) patients managed via contact lenses (CLs) or by both intrastromal corneal rings and contact lenses. (dovepress.com)
  • Data of corneal densitometry and corneal thickness were collected using the Oculus Pentacam at the pretreatment visit and posttreatment visit at 12 months. (dovepress.com)
  • Corneal thickness was found to be statistically significantly different between pre treatment and post treatment for the CL group for central corneal thickness (CCT) and thinnest area ( P =0.01 and P =0.02), respectively. (dovepress.com)
  • The thickness of the altered keratocyte zone was measured on both sides of the LASIK interface. (bmj.com)
  • The total corneal thickness remained unaltered, but epithelial hyperplasia was seen at 2 years. (bmj.com)
  • In addition to these experiments, I will be performing experiments to determine the role of Ctsc (a protein implicated in contributing to corneal thickness) in mouse corneal cells. (csbsju.edu)
  • Anterior OCT demonstrated structural damage of the half of corneal thickness. (escrs.org)
  • Average central corneal thickness is about 550 µm, increasing to about 700 µm in the periphery. (medscape.com)
  • Transplantation of cultured human corneal endothelial cells (HCEC) to treat endothelial dysfunction could help alleviate a worldwide shortage of donor corneas, but a means of definitively proving culture purity must be developed. (arvojournals.org)
  • To determine changes in keratocyte density in central human corneas in vivo after photorefractive keratectomy (PRK). (nih.gov)
  • Primary human keratocytes from 4 donor corneas were cultured in media supplemented with fresh AME (F-AME) or cryopreserved AME (C-AME). (biomedcentral.com)
  • Pallikaris then used the excimer laser ablation on the corneal stromal bed under a hinged flap in rabbit corneas. (medscape.com)
  • Stromal keratocytes from normal human corneas were cultured in the presence of serum. (molvis.org)
  • Over the past two decades, researchers have developed a variety of techniques that can alter corneal surface for refractive purposes or even for halting disease progression in corneas with mechanical decompensation. (hindawi.com)
  • Corneal biomechanics have been assessed in in vitro studies by measuring stress-strain and Young's modulus in isolated corneas [ 8 ]. (hindawi.com)
  • Yet there is a significant shortage of corneas available to transplant, with 10 million people worldwide requiring surgery to prevent corneal blindness as a result of diseases such as trachoma, an infectious eye disorder. (bookofjoe.com)
  • Corneal blindness, which affects millions of people worldwide, is typically treated with transplants of donor corneas, said senior investigator James Funderburgh, Ph.D., professor of ophthalmology at Pitt and associate director of the Louis J. Fox Center for Vision Restoration of UPMC and the University of Pittsburgh , a joint program of UPMC Eye Center and the McGowan Institute for Regenerative Medicine. (upmc.com)
  • The team injected the engineered keratocytes into the corneas of healthy mice, where they integrated without signs of rejection. (upmc.com)
  • He added: 'Worldwide, there is a shortage of suitable corneas for transplantation, and at the present time, there is no effective alternative procedure besides corneal transplantation to treat corneal blindness. (bionews.org.uk)
  • Corneal astigmatism and surface steepness were observed 4 years after BSS implantation, while the implants remained stably integrated in the host corneas. (diva-portal.org)
  • Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. (nih.gov)
  • These changes result in loss of corneal transparency. (nih.gov)
  • Stramer B, Fini M.E. (2004) Uncoupling keratocyte loss of corneal crystallin from markers of fibrotic repair. (kcl.ac.uk)
  • Loss of corneal epithelial heparan sulfate leads to corneal degeneration and impaired wound healing. (sigmaaldrich.com)
  • In most situations, surgical removal (corneal transplantation) is commonly used to restore the vision of patients with opacification, such as in keratoconus, post-infection scarring. (biomedcentral.com)
  • However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. (harvard.edu)
  • Currently only corneal transplantation is able to restore eyesight lost due to corneal scarring. (bionews.org.uk)
  • The biosynthetic stromal substitutes (BSS) characterized herein represent a potentially safe alternative to donor organ transplantation for anterior corneal stromal diseases. (diva-portal.org)
  • Future studies will indicate if biomaterials technology will allow for the optimization of postoperative surface irregularity after anterior stromal replacement, a new window of opportunity that is not available with traditional corneal transplantation techniques. (diva-portal.org)
  • a hypothesis exists that presents excessive keratocyte apoptosis as a major pathological event in keratoconus. (wikipedia.org)
  • Corneal diseases such as keratoconus represent a relatively common disorder in the human population. (harvard.edu)
  • However, in recent years, increasing interest has arisen in corneal biomechanics to predict corneal response to surgical or therapeutic interventions and to assist in the detection of early keratoconus [ 4 - 6 ]. (hindawi.com)
  • Ten patients underwent anterior lamellar keratoplasty using a biosynthetic corneal stromal implant for either advanced keratoconus or central corneal scarring. (diva-portal.org)
  • Corneal confocal microscopy was undertaken to quantify corneal nerve fiber density (CNFD), branch density (CNBD), fiber length (CNFL), keratocyte density (KD), and corneal epithelial basal cell (CEBC) density and area. (elsevier.com)
  • In vivo confocal microscopy demonstrated local area of keratocyte loss, with activation of the surrounding keratocytes. (escrs.org)
  • Addition of TGF(β1) to serum-free cultures resulted in a dramatic transformation of corneal keratocytes to spindle-shaped, fibroblast-like cells that expressed α-SM actin in 100% of cells and exhibited a 20-fold increase in fibronectin synthesis and a 13-fold increase in α 5 β 1 -integrin synthesis. (elsevier.com)
  • Quiescent keratocytes synthesize the so-called crystallins, known primarily for their role in the lens. (wikipedia.org)
  • Apoptosis of keratocytes, either in quiescent or active state, is a process that attracts special attention. (wikipedia.org)
  • Under normal conditions, the keratocytes are quiescent and transparent. (lu.se)
  • Keratocytes create stromal spaces to promote corneal neovascularization via MMP13 expression. (sigmaaldrich.com)
  • To investigate the exact mechanism by which keratocytes promote corneal neovascularization. (sigmaaldrich.com)
  • Although significant progress has been made in recent years, pharmaco-therapeutic agents that promote corneal healing remain limited. (deepdyve.com)
  • BACKGROUND/AIMS The Fas-Fas ligand system is thought to be involved in stromal cell apoptosis after corneal wounding. (bmj.com)
  • 2006. The keratocyte: Corneal stromal cell with variable repair phenotypes. (nih.gov)
  • Despite extensiveresearch on corneal wound healing, corneal scarring remains a major cause of preventable blindness. (diva-portal.org)
  • A study of congenital eye disease at a teaching hospital in Sagamu, Nigeria, found that corneal opacities is the fourth most common congenital eye disorder causing blindness in 5.7% of infants with congenital eye diseases (Bodunde et al, 2006). (aao.org)
  • In addition, almost 5 million people suffer total blindness due to corneal scarring caused by burns, lacerations, abrasion, or disease. (bookofjoe.com)
  • New research by bioengineer David Schmidtke is helping understand how corneal repair cells called keratocytes sometimes result in scarring and blindness. (nih.gov)
  • Experiments conducted by lead author Fatima Syed-Picard, Ph.D., also of Pitt's Department of Ophthalmology, and the team showed that stem cells of the dental pulp, obtained from routine human third molar, or wisdom tooth, extractions performed at Pitt's School of Dental Medicine, could be turned into corneal stromal cells called keratocytes, which have the same embryonic origin. (upmc.com)
  • Furthermore, the transplanted cells were still present after three months and appeared to have assumed the properties of normal corneal cells called keratocytes. (bionews.org.uk)
  • Concepts of corneal refractive surgery, such as keratectomy, keratotomy, and thermokeratoplasty, were first described in 1898 by Lans who published a set of experiments that focused on treating astigmatism in rabbits. (medscape.com)
  • The surgical technique involves the creation of a hinged lamellar corneal flap, after which an excimer laser is used to make a refractive cut on the underlying stromal bed. (medscape.com)
  • Jose Barraquer is generally credited with much of the early work leading to corneal lamellar refractive procedures as they are currently practiced. (medscape.com)
  • Humans are exposed to these organisms on a daily basis, and normal corneal barriers do not allow for an infection to occur. (rareshare.org)
  • In addition, the growth of keratocytes in serum appears to mimic, in part, in vivo activation and myofibroblast transformation. (elsevier.com)
  • Buratto performed excimer laser ablation on the posterior surface of the resected corneal disc before replacing and resuturing it back to its original position. (medscape.com)
  • Excimer laser transepithelial photoablation induces significantly less keratocyte loss than manual epithelial debridement. (google.com)
  • Despite a decrease in concentrations related to reflex tears, the release of sFas and sFasL increases significantly after excimer laser photorefractive keratectomy, which suggests that they are involved in corneal healing after PRK in humans. (bmj.com)
  • 3) Wu WCS, Stark W, Green WR, "Corneal wound healing after 193-nm excimer laser keratectomy. (harvard.edu)
  • To evaluate the participation of proinflammatory cytokines in the acute phase of corneal wound-healing response after excimer laser treatment. (dovepress.com)
  • The acute phase of corneal wound healing after excimer laser treatment is defined by an intensive inflammatory response. (dovepress.com)
  • Pallikaris developed the technique of performing the excimer laser corrective ablation in the corneal stromal bed under a hinged flap. (medscape.com)
  • Crystallins expressed by human keratocytes are ALDH1A1, ALDH3A1, ALDH2 and TKT. (wikipedia.org)
  • The prevalence of congenital corneal opacities (CCO) is estimated to be 3 in100,000 newborns. (aao.org)
  • Table 2 highlights the differential diagnosis of infantile corneal opacities and the method of diagnosis. (aao.org)
  • The aim of this study was to describe morphological changes in a new corneal disease, Dystrophia Smolandiensis, characterized by recurrent corneal erosive episodes and formation of central corneal keloid-like opacities in approximately half of those affected. (diva-portal.org)
  • A ) Histogram plot shows HIPK2 kinase activity in rabbit corneal keratocytes grown in DMEM/F12 medium with and without 10% FBS. (aging-us.com)
  • B , C ) Western blot analysis shows levels of HIPK2, Smad3, p-Smad3, p53, and p-p53 in rabbit corneal keratocytes grown in DMEM/F12 medium with and without 10% FBS. (aging-us.com)
  • D ) QRT-PCR analysis shows relative HIPK2 mRNA levels in rabbit corneal keratocytes transfected with NC-siRNA, HIPK2-siRNA1, HIPK2-siRNA2, and HIPK2-siRNA3 for 48 h. β-actin mRNA levels were used for normalization. (aging-us.com)
  • E ) HIPK2 kinase activity in rabbit corneal keratocytes, transfected with NC-siRNA or HIPK2-siRNA3, and grown in DMEM/F12 medium with 10% FBS for 48 h. (aging-us.com)
  • F , G ) Western blot analysis shows HIPK2, Smad3, p-Smad3, p53, and p-p53 levels in rabbit corneal keratocytes, transfected with NC-siRNA or HIPK2-siRNA3, and grown in DMEM/F12 medium with 10% FBS for 48 h. (aging-us.com)
  • Primary rabbit corneal keratocytes were grown under serum-free conditions or in media supplemented with serum (10% fetal calf serum), TGF(β1) (0.1-10 ng/ml), basic fibroblast growth factor (bFGF) (0.1-10 ng/ml), or heparin (10 U/ml). (elsevier.com)
  • Pubmed ID: 12714623 The purpose of this study was to quantify the effect of mitomycin C on rabbit keratocytes, with a view to determining its potential in modulating corneal stromal wound healing. (jove.com)
  • Pubmed ID: 14691158 To investigate the effects of alcohol and mitomycin C (MMC) on cultured corneal fibroblast of the rabbit to determine the safety of this compound for clinical use. (jove.com)
  • The methods of cell therapy aimed at the restoration of stem cell pool of corneal tissues are the most promising. (deepdyve.com)
  • The purpose of the study was to investigate whether cultured human keratocytes express the neurotensin receptors (NTR1, NTR2, and NTR3), to determine the presence of neurotensin (NT) in keratocytes, and to assess the influence of NT on these cells. (arvojournals.org)
  • Human keratocytes were cultured in medium treated with various concentrations (10 −7 -10 −9 M) of JMV449 (a weakly degradable NT agonist). (arvojournals.org)
  • NT, NTR1, NTR2, and NTR3 mRNA were detected in human keratocytes by means of reverse transcriptase-polymerase chain reaction (RT-PCR). (arvojournals.org)
  • RT-PCR and Western blot analysis showed the expression of the NTR1 (mRNA and protein) and NTR3 mRNA in human corneal keratocytes. (arvojournals.org)
  • JMV449 induced a rapid and transient [Ca 2+ ] i increase in human corneal keratocytes that was blocked by the specific antagonist SR48692. (arvojournals.org)
  • Human corneal keratocytes were isolated by collagenase digestion. (northumbria.ac.uk)
  • It remains unclear if AME from cryopreserved versus fresh human amnion can support human keratocyte propagation, and which components of the extract promote keratocyte growth. (biomedcentral.com)
  • It is feasible to use protein extract from cryopreserved amnion to propagate human keratocytes for potential translational applications. (biomedcentral.com)
  • In the studies of this thesis, it is hypothesized that neuropeptides and neurotransmitters areproduced by human keratocytes and that this production is increased in response to corneal injury. (diva-portal.org)
  • This study sought to identify progenitor cells for human keratocytes. (mendeley.com)
  • These appear to be the first human cells identified with keratocyte progenitor potential. (mendeley.com)
  • Chao, Jennifer R., Bronner, Marianne E. and Lwigale, Peter Y.. "Human Fetal Keratocytes Have Multipotent Characteristics in the Developing Avian Embryo. (rice.edu)
  • however, there have been no studies demonstrating that human corneal keratocytes are multipotent. (rice.edu)
  • The objective of this study is to examine the potential of human fetal keratocytes (HFKs) to differentiate into neural crest-derived tissues when challenged in an embryonic environment. (rice.edu)
  • The injected keratocytes were detected by immunofluorescence using the human cell-specific marker, HuNu. (rice.edu)
  • To quantify human corneal recovery after moderate to high myopic laser in situ keratomileusis (LASIK) in a 2-year prospective follow-up study. (bmj.com)
  • TGF-ß3 stimulates stromal matrix assembly by human corneal keratocyte-like cells. (bu.edu)
  • The goal of this phase 1 safety study was to characterize the three-dimensional (3D) corneal shape of the first 10 human patients implanted with a BSS and assess its stability over time. (diva-portal.org)
  • Near the corneal limbus, stromal cells expressed ABCG2, a protein present in many adult stem cells. (mendeley.com)
  • Both phase contrast micrographs and gene expression analysis confirmed the corneal keratocytes retained their phenotypes with the addition of EBN. (biomedcentral.com)
  • The cells were either processed immediately (i.e. freshly isolated keratocytes) or were cultured in the presence of 10% fetal bovine serum or transforming growth factor-β to induce transformation to the corneal fibroblast and myofibroblast phenotypes, respectively. (northumbria.ac.uk)
  • It is concluded that both the connexin mRNA and protein are expressed in central corneal and limbal epithelia [ 10 ]. (hindawi.com)
  • Expression and secretion of SP in cultured keratocytes wasincreased in response to injury in vitro. (diva-portal.org)
  • Corneal keratocytes: phenotypic and species differences in abundant protein expression and in vitro light-scattering. (semanticscholar.org)
  • Some keratocytes underlying the site of injury, even a light one, undergo apoptosis immediately after the injury. (wikipedia.org)
  • This study aimed to investigate the effects of EBN on corneal keratocytes proliferative capacity and phenotypical changes. (biomedcentral.com)
  • This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. (blogspot.com)
  • Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. (blogspot.com)
  • CONCLUSIONS: Coadministration of TTX and PPC significantly prolonged corneal anesthesia, but in view of delayed corneal reepithelialization, caution is suggested in the use of the drug combination. (biomedsearch.com)
  • Immunohistochemistry using antibodies specific for UBIAD1 protein in keratocytes revealed that both wild type and N102S protein were localized sub-cellularly to mitochondria. (pubmedcentralcanada.ca)
  • Densitometry of one-dimensional gels revealed that no single protein isoform exceeded 5% of the total water-soluble protein fraction, which is the qualifying property of a corneal enzyme-crystallin according to the current definition. (lu.se)
  • Higher thermal doses produced a spectrum of damage, with complete destruction of all keratocytes and endothelial cells at 59 degrees C X 45 min. (mendeley.com)
  • There has been no effective treatment or agent that is available for corneal injury in promoting corneal wound healing. (biomedcentral.com)
  • The process of corneal wound healing is complex. (diva-portal.org)
  • However, when the process of corneal wound healing is incompleteor disturbed, corneal scarring may occur, which can lead to significantly impaired vision. (diva-portal.org)
  • Further analysis of these cells will aid elucidation of molecular mechanisms of corneal development, differentiation, and wound healing. (mendeley.com)
  • 8 Wilson et al suggested that apoptosis is a primary step in corneal wound healing, possibly affecting the outcome of PRK. (bmj.com)
  • To further extend the finding, we determined whether HuR expression affects β-actin gene expression and in turn affects corneal fibroblast migration and wound healing. (molvis.org)
  • Mitomycin C (MMC) was proposed to be an effective modulator of the wound healing response after corneal stromal ablation as early as 1991. (pubmedcentralcanada.ca)
  • Acacia honey is a natural product which has proven to have therapeutic effects on skin wound healing, but its potential healing effects in corneal wound healing have not been studied. (blogspot.com)
  • The results of the present study show promising role of AH role in accelerating the initial stage of corneal wound healing. (blogspot.com)
  • Minns MS, Trinkaus-Randall V. Purinergic Signaling in Corneal Wound Healing: A Tale of 2 Receptors. (bu.edu)
  • This study aimed to elucidate the role of HS in corneal epithelial homeostasis and wound healing. (sigmaaldrich.com)
  • The conditional knockout of HS from keratocytes using the keratocan promoter led to no corneal abnormalities or any disruption in wound healing. (sigmaaldrich.com)
  • These findings demonstrate that epithelial-derived exosomes may be involved in corneal wound healing and neovascularization, and thus, may serve as targets for potential therapeutic interventions. (harvard.edu)
  • Cloned SP cells cultured in medium with fibroblast growth factor-2 lost ABCG2 and PAX6 expression and upregulated several molecular markers of keratocytes, including keratocan, aldehyde dehydrogenase 3A1, and keratan sulfate. (mendeley.com)
  • They are thought to accumulate because of an inability to break down corneal keratan sulfate. (harvard.edu)
  • The main aim of this thesis was to investigate two hereditary corneal diseases with recurrent erosions in order to find out if they had been described before, and more specifically to describe the clinical picture and the morphological changes, differentiate them from other known autosomal dominant corneal dystrophies with a clinical resemblance, and to exclude genetic linkage to known corneal dystrophies with autosomal-dominant inheritance and a clinical resemblance. (diva-portal.org)
  • This may correlate with the observed decrease in the density of the most anterior keratocytes. (bmj.com)
  • Additionally, increasing interest has also arisen in corneal biomechanical properties and glaucoma once corneal biomechanics have been shown to influence intraocular pressure (IOP) measurements and may be also indicative of ocular globe biomechanics that could also be predictive of glaucoma susceptibility [ 7 ]. (hindawi.com)
  • We show that such a model can extend predictive accuracy in corneal biomechanics. (royalsocietypublishing.org)
  • When corneal fibrosis is considered secondary to inflammation, it should not be diagnosed separately unless warranted by its severity, though it should be described in the pathology narrative. (nih.gov)
  • Fibronectin was expressed in the area of subepithelial fibrosis, and the keratocytes in this area generally expressed S100A4. (diva-portal.org)
  • Corneal topography revealed 8 diopters irregular astigmatism. (escrs.org)
  • Our results suggest a potential treatment regimen for congenital and/or acquired corneal diseases. (bionews.org.uk)
  • Specimens of one primary corneal graft, one regraft, and one biopsied keloid-like region, obtained from members of a large family with the disease, were re-examined with a light microscope, and sections were stained with Congo red and immunohistochemically analyzed for fibronectin and S100A4. (diva-portal.org)
  • The aim of this paper was to develop a GFP-expressing transgenic mouse model for the keratoepithelioplasty and to use this to follow the outcome of this form of graft, when placed on an inflamed corneal surface. (ebscohost.com)