The transparent anterior portion of the fibrous coat of the eye consisting of five layers: stratified squamous CORNEAL EPITHELIUM; BOWMAN MEMBRANE; CORNEAL STROMA; DESCEMET MEMBRANE; and mesenchymal CORNEAL ENDOTHELIUM. It serves as the first refracting medium of the eye. It is structurally continuous with the SCLERA, avascular, receiving its nourishment by permeation through spaces between the lamellae, and is innervated by the ophthalmic division of the TRIGEMINAL NERVE via the ciliary nerves and those of the surrounding conjunctiva which together form plexuses. (Cline et al., Dictionary of Visual Science, 4th ed)
Fluorescent Antibody Technique, Indirect
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
A sulfated mucopolysaccharide initially isolated from bovine cornea. At least two types are known. Type I, found mostly in the cornea, contains D-galactose and D-glucosamine-6-O-sulfate as the repeating unit; type II, found in skeletal tissues, contains D-galactose and D-galactosamine-6-O-sulfate as the repeating unit.
Corneal Dystrophies, Hereditary
An enzyme that, in the pathway of cholesterol biosynthesis, catalyzes the condensation of isopentenyl pyrophosphate and dimethylallylpyrophosphate to yield pyrophosphate and geranylpyrophosphate. The enzyme then catalyzes the condensation of the latter compound with another molecule of isopentenyl pyrophosphate to yield pyrophosphate and farnesylpyrophosphate. EC 18.104.22.168.
Biological Science Disciplines
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Directories as Topic
POU Domain Factors
A family of transcription factors characterized by the presence of a bipartite DNA-binding domain known as the POU domain. The POU domain contains two subdomains, a POU-specific domain and a POU-homeodomain. The POU domain was originally identified as a region of approximately 150 amino acids shared between the Pit-1, Oct-1, Oct-2, and Unc-86 transcription factors.
Genes that encode highly conserved TRANSCRIPTION FACTORS that control positional identity of cells (BODY PATTERNING) and MORPHOGENESIS throughout development. Their sequences contain a 180 nucleotide sequence designated the homeobox, so called because mutations of these genes often results in homeotic transformations, in which one body structure replaces another. The proteins encoded by homeobox genes are called HOMEODOMAIN PROTEINS.
Insulin growth factor promotes human corneal fibroblast network formation in vitro. (1/66)(+info)
Polyethylenimine-conjugated gold nanoparticles: Gene transfer potential and low toxicity in the cornea. (2/66)(+info)
Synergistic induction of eotaxin and VCAM-1 expression in human corneal fibroblasts by staphylococcal peptidoglycan and either IL-4 or IL-13. (3/66)(+info)
An explanation for the central to peripheral thickness variation in the mouse cornea. (4/66)(+info)
Localization of thrombospondin-1 and myofibroblasts during corneal wound repair. (5/66)(+info)
Hydroxyapatite for keratoprosthesis biointegration. (6/66)(+info)
Assessment of corneal thickness and keratocyte density in a rabbit model of laser in situ keratomileusis using scanning laser confocal microscopy. (7/66)(+info)
Coxsackievirus A24 variant uses sialic acid-containing O-linked glycoconjugates as cellular receptors on human ocular cells. (8/66)(+info)
FGF-2- and TGF-β1-Induced Downregulation of Lumican and Keratocan in Activated Corneal Keratocytes by JNK Signaling Pathway |...
The results of the JNK inhibitor studies were verified using DsiRNA to knockdown JNK1 and JNK2 in the cells. FGF-2/HS-induced activation of keratocytes, transfected with scrambled DsiRNA (controls), resulted in a 140% ± 40% and a 130% ± 20% increase in JNK1 and JNK2 mRNA levels, respectively (Fig. 5). The levels of lumican and keratocan in these cells were reduced by 65% ± 3% and 45% ± 15%, respectively. When nonactivated keratocytes were transfected with JNK1/2 DsiRNA, the levels of JNK1 and JNK2 mRNAs were reduced by 53% ± 6% and 66% ± 7%, respectively. The levels of lumican and keratocan in these cells were 27% ± 5% and 44% ± 6% higher, respectively, than those in corresponding scrambled DsiRNA-transfected controls. Similarly, when the keratocytes, transfected with JNK1/2 DsiRNA, were activated with FGF-2/HS, the levels of JNK1 and JNK2 mRNAs were reduced by 75% ± 13% and 57% ± 8%, respectively. The levels of lumican and keratocan in these cells were 70% ± 20% and 100% ± 10% ...
Thy-1 distinguishes human corneal fibroblasts and myofibroblasts from keratocytes - Northumbria Research Link
After corneal injury, keratocytes become activated and transform into repair phenotypes-corneal fibroblasts or myofibroblasts, however, these important cells are difficult to identify histologically, compromising studies of stromal wound healing. Recent studies indicate that expression of the cell surface protein, Thy-1, is induced in fibroblast populations associated with wound healing and fibrosis in other tissues. We investigated whether keratocyte transformation to either repair-associated phenotype induced Thy-1 expression. Human corneal keratocytes were isolated by collagenase digestion. The cells were either processed immediately (i.e. freshly isolated keratocytes) or were cultured in the presence of 10% fetal bovine serum or transforming growth factor-β to induce transformation to the corneal fibroblast and myofibroblast phenotypes, respectively. Thy-1 mRNA and protein expression by freshly isolated keratocytes and corneal fibroblasts were assessed by RT-PCR and Western blotting. mRNA ...
MMP14-Mediated Differential Regulation of VEGFR1, 2, & 3 Expression in Corneal Epithelial Cells vs. Corneal Keratocytes | IOVS ...
Purpose : Vascular endothelial growth factor receptor 3 (VEGFR3) regulates the growth and differentiation of blood and lymphatic vessels. We hypothesized that matrix metalloproteinase 14 (MMP14)-modulated VEGFR3 expression in the corneal epithelium may influence corneal avascularity. Methods : VEGFR3 expression was compared between wild-type and MMP14-deficient (MMP14 Δexon4) corneal epithelial cells and keratocytes. VEGFR3 protein and mRNA expression were measured by Western blot and quantitative RT-PCR analyses, respectively. Potential cleavage of VEGFR3 by catalytic MMP14 was examined in an in vitro proteolysis experiment. Mass proteomic analysis was used to identify potential nuclear proteins differentially expressed in MMP14 Δexon4 and WT corneal epithelial cells. Results : VEGFR3 protein expression was much higher on MMP14 Δexon4 corneal epithelial cells than on wild-type cells. Additionally, quantitative RT-PCR analysis showed that VEGFR3 gene expression was highly induced in MMP14 ...
Proteome analysis of serum-cultured human corneal fibroblasts</em>...
TY - CONF. T1 - Proteome analysis of serum-cultured human corneal fibroblasts. AU - Karring, Henrik AU - Thøgersen, Ida B. AU - Klintworth, Gordon K. AU - Enghild, Jan J. AU - Møller-Pedersen, Torben. PY - 2004. Y1 - 2004. M3 - Poster. Y2 - 8 November 2004 through 12 November 2004. ER - ...
All keratocytes participate in sphere formation. A: Fre | Open-i
All keratocytes participate in sphere formation. A: Freshly isolated primary keratocytes were cultured in polyHEMA-coated dishes either in Advanced-DMEM (ADV, d
Lamella of keratocytes generate a small rearward tracti | Open-i
Lamella of keratocytes generate a small rearward traction force against the micromachined substratum. Bar, 5 μm. Once the lamella of a keratocyte is over the m
Backlash - Austin Public Library
Gr 7 Up — For sophomore Lara Kelly, things are finally looking up—shes feeling more confident after losing weight and she made the varsity cheerleading team, which she never would have imagined two years earlier when she was overweight and severely depressed. Best of all, Lara has caught the attention of a cute guy on Facebook, and he has been hinting at asking her to the homecoming dance. But when she sees horrible comments from her crush on social media, she spirals into a dangerous mental state and suicide seems like the only escape. Bree is Laras former best friend from middle school, but they drifted apart when Bree couldnt take Laras depression and self-involvement. The new Lara is suddenly getting everything that Bree is supposed to have—the popularity, and even the spot on the cheer team. Sydney and Liam are the younger siblings, who are caught up in the horror of a tragic event, and trying to figure out how to cope with their siblings issues while living their own ...
When Corneal Wounds Wont Heal
1. Jester J, Ho-Chang J. Modulation of cultured corneal keratocyte phenotype by growth factors/cytokines control in vitro contractility and extracellular matrix contraction. Exp Eye Res. 2003;77(5):581-92.. 2. Myrna KE, Pot SA, Murphy CJ. Meet the corneal myofibroblast: the role of myofibroblast transformation in corneal wound healing and pathology. Vet Ophthalmol. 2009;12 Suppl 1:25-7.. 3. Remingto, L. Cornea and Sclera. In: Clinical Anatomy of the Visual System. 3rd ed. St. Louis, Mo: Elsevier, 2012.. 4. Alio J, Rodriguez A, Ferreira-oliveira R, et al. Treatment of dry eye disease with autologous platelet-rich plasma: a prospective, interventional, non-randomized study. Ophthalmol Ther. 2017;6(2):285-93.. 5. Alio JL, Rodriguez AE, Abdelghany AA, et al. Autologous platelet-rich plasma eye drops for the treatment of post-LASIK chronic ocular surface syndrome. J Ophthalmol. 2017;2017:2457620.. 6. Anitua E, Muruzabal F, De la fuente M, et al. Plasma rich in growth factors for the treatment of ...
Human Fetal Keratocytes Have Multipotent Characteristics in the Developing Avian Embryo
The human cornea contains stem cells that can be induced to express markers consistent with multipotency in cell culture; however, there have been no studies demonstrating that human corneal keratocytes are multipotent. The objective of this study is to examine the potential of human fetal keratocytes (HFKs) to differentiate into neural crest-derived tissues when challenged in an embryonic environment. HFKs were injected bilaterally into the cranial mesenchyme adjacent to the neural tube and the periocular mesenchyme in chick embryos at embryonic days 1.5 and 3, respectively. The injected keratocytes were detected by immunofluorescence using the human cell-specific marker, HuNu. HuNu-positive keratocytes injected along the neural crest pathway were localized adjacent to HNK-1-positive migratory host neural crest cells and in the cardiac cushion mesenchyme. The HuNu-positive cells transformed into neural crest derivatives such as smooth muscle in cranial blood vessels, stromal keratocytes, and ...
Browsing Electronic Theses and Dissertations by Subject keratocytes
Purpose: Accidental or surgical-induced trauma (e.g., refractive surgery) to the corneal epithelium results in keratocyte death immediately below the wound. Subsequent recovery of keratocytes during healing often remains ...
CIL:12013, Hypsophrys nicaraguensis, keratocyte. CIL. Dataset
Spontaneous symmetry breaking and motility initiation of keratocytes. Phase-contrast time-lapse video of a keratocyte spontaneously breaking symmetry ...
Official ESCRS | European Society of Cataract & Refractive Surgeons
A total of 18 patients were recruited with similar bilateral refraction. The patients underwent SMILE in one eye and FS-LASIK in the other eye. After detailed clinical examination, patients underwent imaging on Pentacam HR and Corvis-ST before and after surgery (1, 3 and 6month). Corneal tomography from Pentacam-HR was used to construct 3-D corneal model and mesh.The I-FEM derived the best fit material properties to describe the corneal deformation measured by Corvis-ST.Using the computed properties, stress strain analogue for each measurement was formulated by simulating intraocular pressure(IOP; 0-60mmHg).The displacement of the corneal apex was calculated from stress-strain analogue ...
wst1 blank absorbance problem: post #1
wst1 blank absorbance problem - posted in Tissue and Cell Culture: Hi everyone, Recently, Ive tried wst1 assay for the first time. After treating the cells with chemical, i added 100 ul fresh DMEM containing 10 ul wst1 and incubated for 3 hours. Ive measured the absorbance in 355-460 interval (this was the closest filter pair to 450 nm -that I could choose in our reader-). Unexpectedly, I got the maximum absorbance values in my blank samples (DMEM+wst1; without cells) and positive controls...
Review: Herculin Extreme Pre-Workout by EBN
The Rx Review: Reporting on Fitness and CrossFit News, John Michael Bric This week we take a moment to review the Herculin Extreme Pre-Workout supplement by EBN Sports Nutrition. Item: Herculin Extreme Pre-Workout Price: $54 USD (450 grams) Retailer: EBN Sports Nutrition Purchase: www.EBNSports.com The current supplement market is flooded with hundreds of different pre-workout products. Many of which claim to…
Quantitative assessment of anteroposterior keratocyte density in the normal rabbit cornea<...
TY - JOUR. T1 - Quantitative assessment of anteroposterior keratocyte density in the normal rabbit cornea. AU - Petroll, W. M.. AU - Boettcher, K.. AU - Barry, P.. AU - Cavanagh, D.. AU - Jester, J. V.. PY - 1995. Y1 - 1995. N2 - The anteroposterior keratocyte density distribution in the rabbit cornea was measured. Unsectioned tissue blocks from the central cornea of five rabbits were stained with propidium iodide and imaged using a Leica laser scanning confocal microscope. A z-series of images was acquired in each sample, from anterior to posterior stroma in either 3- or 8-μm steps. Software was developed to allow interactive marking of the keratocyte nuclei within each section of the z-series and for calculating cell density. For convenience, cell density was expressed as the number of cells per corneal volume element (CVE), where CVE is a newly defined volume unit with x, y, and z dimensions of 250, 250, and 10 μm, respectively. The calculated keratocyte density was 20.2 ± 1.0 cells/CVE (n ...
Data for Lasik Surgical Treatment Patients - Blog
Lots of ophthalmologic cosmetic surgeons are currently supplied the next evolution in LASIK surgical treatment, which they called Customized LASIK. The general treatment used to correct refractive mistakes coincides as is used for conventional LASIK treatments, with the enhancement of wavefront devices made use of to map the distinct surface area of the clients eye. The wavefront tool passes a narrow ray check out here of light through the systems as well as into the eye, measuring any optical distortions that result as the light leaves back out the eye. Any kind of abnormalities recognized are after that fed back right into the system so that the laser can automatically deal with for aberrations on the surface of the eye. Proponents of Personalized LASIK case that the use of wavefront modern technology allows cosmetic surgeons to deal with greater order aberrations that could add to low-light aesthetic disruptions such as halos as well as glare not corrected by conventional LASIK, eyeglasses, ...
Topical drug formulations for prolonged corneal anesthesia.
Ocular local anesthetics currently used in routine clinical practice for corneal anesthesia are short acting and their ability to delay corneal healing makes them unsuitable for long-term use. In this study, we examined the effect of the site 1 sodiu
The Importance of Dr Tooma Lasik | Sports LASIK
The cornea isnt a parcel of plastic. Conventional LASIK has become the most common kind of laser vision correction, and it has proven to be very effective for the correction of conventional vision errors. Blade Free LASIK (iLASIK) Imagine having the ability to perform most or all your activities of everyday life without the load…
GeneTiCA je dodavatel moderních přístrojů, reagencií, spotřebního materiálu a speciálních diagnostik pro biomedicínský výzkum a diagnostické aplikace
Congenital corneal anaesthesia and the MURCS association: a case report. | British Journal of Ophthalmology
Bilateral corneal anaesthesia and reduced sensation in the distribution of the ophthalmic division of the fifth cranial nerve associated with neuroparalytic keratitis and reduced vision is described in a 26-year-old woman with absence of the fallopian tubes, uterus, and upper vagina, a single pelvic kidney, and cervico-thoracic vertebral anomalies (the MURCS association). Other features of the disorder also present were short stature, facial asymmetry, micrognathia, and cleft palate. The neuroparalytic keratitis did not respond to tear substitutes and bilateral lateral tarsorrhaphies were eventually needed. This is the first report of congenital corneal anaesthesia in a case of MURCS. ...
LASIK Eye Surgery | Rand Eye Institute
The advanced surface procedure does not require the creation of a corneal flap. Instead, the superficial (epithelial) layer of the cornea is simply brushed aside and the excimer laser is applied to the surface to reshape the corneal tissue. This epithelial tissue usually regrows to cover the surface within a few days.. The biggest advantage of no-flap laser vision correction is the elimination of most of the flap-related risks. Most conventional LASIK is usually a safe and complication-free experience, but there are certain risks associated with a corneal flap. Chronic dryness, distorted vision from flap misplacement and early and late complications due to the structural weakness related to the corneal flap. With no-flap laser vision correction, these risks are virtually eliminated.. No-flap laser vision correction is a great option for many patients who do not qualify for conventional LASIK because they have thin corneas, or have had previous LASIK. For this reason, the military forces approved ...
Transparency of the bovine corneal stroma at physiological hydration and its dependence on concentration of the ambient anion ...
De-epithelialised and de-endothelialised bovine corneal stromas with a hydration of 3.2 equilibrated at 154 mM NaCl and buffered at pH 7.4 had their optical density (400-750 nm) measured. Stromas equilibrated against 10, 20, 30, 50 or 100 mM NaCl made isotonic to 154 mM NaCl by supplementing with sorbitol were progressively more transparent as NaCl increased. Hypertonic equilibration against 300, 600 or 1000 mM NaCl resulted in a progressive loss of transparency compared with 154 mM NaCl. Light scattering as a function of wavelength fitted a l_3 function well for 10, 30, 50, 100 and 154 mM NaCl preparations between 450 and 650 nm, but not at higher wavelengths. However, hypertonic 300, 600 and 1000 mM NaCl preparations showed a l_2 dependence in the 450-750 nm range. Experiments with 154 mM NaCl and either 0 or 300 mM sorbitol suggested that the changes in light scattering in hypertonic preparations are unlikely to be caused by osmotic alterations to the stromal keratocytes. Psychophysical ...
RESULTS: The receptors for LXA4, ALX/Fpr-rs-2 and for RvE1, ChemR23 were each expressed by epithelium, stromal keratocytes, and infiltrated CD11b(+) cells in corneas. Compared to the vehicle-treated eye, ATLa-, RvD1-, and RvE1-treated eyes had reduced numbers of infiltrating neutrophils and macrophages and reduced mRNA expression levels of TNF-alpha, IL-1 alpha, IL-1 beta, VEGF-A, VEGF-C, and VEGFR2. Animals treated with these mediators had significantly suppressed suture-induced or IL-1 beta-induced hemangiogenesis (HA) but not lymphangiogenesis. Interestingly, only the application of ATLa significantly suppressed VEGF-A-induced HA ...
Keratan sulphate is unevenly distributed from back to front of bovine cornea | Biochemical Society Transactions
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
Inhibition of lymphocyte proliferation by resident ocular cells
The mechanisms by which the eye maintains an immunosuppressive environment has been the subject of recent investigations. In this report we investigated the ability of resident ocular cells from the iris, choroid, and retina to inhibit lymphocyte responses in vitro. Our results demonstrate that sing …
Difference between revisions of Template:Frog Cornea Timeline table - Embryology
second wave of migratory cells containing presumptive keratocytes invades the matrix, leading to formation of inner cornea and outer cornea. A unique cell mass (stroma attracting center) connects the two layers like the center pole of a tent ...
Wstęp - Deep Learning [Book]
Wstęp Krótka historia uczenia głębokiego Korzenie obecnej popularności uczenia głębokiego sięgają zaskakująco głęboko, bo aż do lat 50. ubiegłego wieku. Choć mgliste wzmianki o „inteligentnych maszynach
Lysophosphatidic acid activates TGFBIp expression in human corneal fibroblasts through a TGF-β1-dependent pathway<...
TY - JOUR. T1 - Lysophosphatidic acid activates TGFBIp expression in human corneal fibroblasts through a TGF-β1-dependent pathway. AU - Jeon, Eun Su. AU - Kim, Jae Ho. AU - Ryu, Hyunmi. AU - Kim, Eung Kweon. PY - 2012/6/1. Y1 - 2012/6/1. N2 - Granular corneal dystrophy type 2 (GCD2) is an autosomal dominant disease caused by a R124H point mutation in the transforming growth factor-β-induced gene (TGFBI). However, the cellular role of TGFBI and the regulatory mechanisms underlying corneal dystrophy pathogenesis are still poorly understood. Lysophosphatidic acid (LPA) refers to a small bioactive phospholipid mediator produced in various cell types, and binds G protein-coupled receptors to enhance numerous biological responses, including cell growth, inflammation, and differentiation. LPA levels are elevated in injured cornea and LPA is involved in proliferation and wound healing of cornea epithelial cells. Accumulating evidence has indicated a crucial role for LPA-induced expression of TGFBI ...
Bandage Lenses Validated as Post-LASIK Treatment
Bandage contact lenses (BCL) may help reduce pain and result in a less intense wound healing response following femtosecond laser in situ keratomileusis (FS-LASIK), according to a study in the Journal of Ophthalmology.. This prospective, randomized trial included 41 patients (82 eyes) with myopia and/or myopic astigmatism. After FS-LASIK surgery, patients were fitted with a BCL in one eye but not in the contralateral eye. The BCL was left in place overnight and removed the next morning. All eyes subsequently received standardized postoperative treatments. Investigators followed patients for six months and evaluated the subjects self-reported postoperative symptom scores for pain, photophobia, tearing and foreign-body sensation. At six months after surgery, researchers examined the corneal flap margin and adjacent regions and photographed them using slit-lamp biomicroscopy to subjectively evaluate the wound healing response.. The study reported postoperative pain and photophobia were milder in ...
Fixing and staining tissue culture cells or slices grown in a 3D collagen matrix with fluorescent phalloidin
Actin stains and probes are useful biological reagents for detecting and visualizing actin in cells and tissues, actin staining with Acti-stain 488 phalloidin is the best option for bright and stable fluorescence imaging, fluorescent actin, actin antibody, stem cell stain, stem cell marker, sarcomere stain, muscle cell stain, fluorescent actin.
CIL:35062. CIL. Dataset
Electron micrograph of keratocyte or fibroblast lamellipodial actin network after unprotected extraction. All examples demonstrate frequent branching...
Wyspa Złych Ludzi: Słowem wstępu
Powstał z nudów więc z nudów pewnie będę na nim pisał. Tak naprawdę ten blog to taki wybieg w przyszłość - jak się w sobie zbiorę i zrobię do końca to co leży w szufladzie od jakiś 3 lat to prawdopodobnie blog będzie czymś w stylu zaplecza ...
EBN | Organisation profile
Literatur zu EbN / EbM
Bartoszek, G., Gnass, I., Möhler, R., Dobrin-Schippers, A. & Meyer, G. (2010). Einführung in Evidence-based Nursing am Beispiel der Wirksamkeitsbewertung Medizinischer Thromboembolieprophylaxestrümpfe zur Vermeidung tiefer Beinvenenthrombosen nach Schlaganfall. Zeitschrift Pflegenetz, 1, 4 - 7.Artikel nur online einsehbar unter ...
Farmaceutyczne zbrodnie: HIV, pryszczyca, Hitler i testy na dzieciach. | Tergiwersacja
Farmaceutyczne zbrodnie: HIV, pryszczyca, Hitler i testy na dzieciach. Koncerny farmaceutyczne są czyste i przejrzyste jak hipotetyczny tandem NSDAP z KPZR. Na wstępie omawiany już Baxter. Reakcja alergiczna na ich lek heparyn przyniosła od stycznia 2007 roku do kwietnia 2009 roku 103 zabitych… Stało się to powodem pozwu sądowego Joyce Ann Osteen złożonego 5 stycznia…
Tytu : Badania por wnawcze wp ywu k pieli wodnych z ozonem i k pieli bez ozonu skojarzonych z kinezyterapi na dolegliwo ci b lowe, wydolno czynno ciow staw w kolanowych oraz perfuzj mi ni ko czyn dolnych u os b z chorob zwyrodnieniow staw w kolanowych (doniesienie wst pne). ...
An unusual presentation of diabetic neuropathy | British Journal of Ophthalmology
Corneal anaesthesia can be physiological or pathological. Corneal sensation decreases with age, and is lower in females, especially premenstrually. Contact lens wear, and infection by herpes zoster and simplex, oedema and surgery will also reduce sensation. Congenital causes of corneal anaesthesia include corneal dystrophy and Riley-Day syndrome, and congenital corneal anaesthesia without an associated syndrome, which is presumed to be due to hypoplasia of the ophthalmic division of the trigeminal nerve. Systemic disease such as diabetes, myotonic dystrophy, scleroderma, and vitamin deficiencies are important causes of corneal anaesthesia, which can often be overlooked. Forty five per cent of diabetic patients had a degree of corneal hypoaesthesia when examined in a study of 130 patients published by Osman et al.1 There is little or no relation between the age of a diabetic patient and the observed decrease in corneal sensitivity. However, corneal sensitivity thresholds do rise with increased ...
Analysis of the pattern of subcellular force generation by corneal fibroblasts after Rho activation<...
TY - JOUR. T1 - Analysis of the pattern of subcellular force generation by corneal fibroblasts after Rho activation. AU - Petroll, W. Matthew. AU - Ma, Lisha. AU - Ly, Linda. AU - Vishwanath, Mridula. N1 - Copyright: Copyright 2008 Elsevier B.V., All rights reserved.. PY - 2008/1. Y1 - 2008/1. N2 - PURPOSE. To determine the structural and subcellular mechanical effects of Rho activation on corneal fibroblasts in three-dimensional collagen matrices. METHODS. Human corneal fibroblasts were plated at low density in 100-μm thick fibrillar collagen matrices and cultured for 1 or 2 days in serum-free media. Time-lapse imaging was then performed at 1- to 2-minute intervals with Nomarski differential interference contrast. After 1 hour, perfusion was switched to serum-free media containing 1 μmol/L lysophosphatidic acid (LPA). After an additional 30 to 60 minutes, the Rho kinase (ROCK) inhibitor Y-27632 was added to the perfusion media. Changes in cell structure and extracellular matrix deformation ...
Small Incision Lenticule Extraction<...
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Official ESCRS | European Society of Cataract & Refractive Surgeons
there was no statistically significant difference between both groups in �Î� Q value with Fs-LASIK inducing more oblate anterior corneal shape (P = 0.2124). Regarding corneal higher order aberrations, there was no statistically significant difference in the change in RMS of total corneal higher order aberration (P = 0.2) with more change of RMS of total aberrations in SMILE group .There was no statistically significant difference in RMS of coma aberration(P = 0.0589) ,however SMILE group showed more change in coma aberration. There is no statistically significant difference in RMS of spherical aberration (P = 0.0543 ...
Periocular Mesenchymal Cells Development in the Periocular Mesenchyme - LifeMap Discovery
Periocular mesenchymal cells are a subpopulation of the cranial neural crest, located near the developing retina and lens. Some of the periocular mesenchymal cells migrate into the region between the lens vesicle and the developing cornea epithelium and give rise to corneal endothelium and stromal keratocytes ...
The Effects Of Vitamin A Compounds On Hyaluronic Acid Released From Cultured Rabbit Corneal Epitheli | Ray Peat Forum
The effects of vitamin A compounds on hyaluronic acid released from cultured rabbit corneal epithelial cells and keratocytes. - PubMed - NCBIA role...
products : glass bottom dishes and 6/12/24/96/384 well glass bottom plates
Chambered cover glass /Glass bottom dishes / glass bottom plates at Cellvis (formerly In Vitro Scientific) for confocal microscopy studies. ideal for fluorescence microscopy.
WinSanTor is developing a proprietary first-in-class therapy to prevent and reverse nerve damage. Our lead compound, WST57, is a well-characterized off-patent compound with an established safety history that has been used for many years against an unrelated indication, this may allow a direct path to market and reducing development time and cost.. WST57 prevents, mitochondrial dysfunction, nerve fiber depletion and sensory loss in several distinct animal models of peripheral neuropathy.. We are strategically expanding WST57s indications to all neuropathies, such as chemo induced and HIV related. Additionally, WST57 is just one compound we are investigating in a family of promising compounds that we have exclusive world wide rights to.. ...
WST 114: Lactate Conventionally - Sid Garza-Hillman
The conventional wisdom around lactic acid is wrong. So is what theyre charging for heirloom tomatoes. Plus, bad vegan comedy and more.. Matt Fitzgerald Article on Lactic Acid/Lactate. SMALL STEP INTENSIVE PRIVATE COACHING ...
Japan-chin - inzerce
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Str nky o kynologii, psech, jejich v chov a v cviku, veterin , kynologick diskuse, odkazy na dal str nky o psech, katalog ps , on-line inzerce, kynologick literatura, n zory, wallpapery, zku ebn dy, kalend kynologick ch akc
Get PDF - Full-Thickness Astigmatic Keratotomy Combined With Small-Incision Lenticule Extraction to Treat High-Level and Mixed...
Kim, B.Ki.; Mun, S.Joung.; Lee, D.Gyu.; Kim, J.Ryun.; Kim, H.Seung.; Chung, Y.Taek., 2015: Full-Thickness Astigmatic Keratotomy Combined With Small-Incision Lenticule Extraction to Treat High-Level and Mixed Astigmatism
Ogromny tętniak rzekomy aorty wstępującej po 13 latach od operacji Bentalla z wszczepieniem cylindrycznego konduitu | Ahmadi |...
Tętniaki rzekome aorty wstępującej są rzadkimi, katastrofalnymi powikłaniami zabiegów chirurgicznych przeprowadzanych na aorcie i w jej okolicy. Wymagają one często interwencji chirurgicznej w trybie nagłym ze względu na duże ryzyko nagłego pęknięcia i krwotoku. W pracy przedstawiono przypadek tętniaka rzekomego (o średnicy 130 mm) aorty wstępującej u 34- -letniego mężczyzny, który uciskał lewy przedsionek i miejsce odejścia prawej tętnicy wieńcowej, a znajdował się w miejscu cylindrycznego konduitu wszczepionego 13 lat wcześniej podczas operacji Bentalla. Skłonność tych tętniaków rzekomych do niemego klinicznie powiększania się w ciągu wielu lat prowadzi do opóźnienia rozpoznania, co wiąże się ze zwiększonym ryzykiem pęknięcia i zgonu. Konieczna jest więc długoterminowa obserwacja takich chorych, aby można było wykrywać opisane zmiany w początkowych stadiach, kiedy łatwiej wykonywać interwencje chirurgiczne lub wewnątrznaczyniowe z mniejszym ...
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Substratum topography modulates corneal fibroblast to Myofibroblast transformation - Fingerprint - UC Davis
Wusunové - Wikipedie
Archeologické naleziště z období pobytu Wu-sunů v Ťan-šanu a Žety-su jsou různorodá a odráží různorodé etnické složení obyvatelstva. Archeologickými výzkumy v bývalém Sovětském svazu bylo na severním břehu řeky Ili objeveno velké množství Wu-sunských hrobů. Většina hřbitovů, které musely patřit Sakům zahrnutých v konfederaci, si zachovala tradice obřadů a pohřební hmotnou kulturu dřívějších Saků. S Wu-suny jsou taky spojovány hromady lineárních „podzemních pohřebních prostorů komnatových hrobů. Jednalo se o zbraně, v některých případech o bohaté šperky a ozdoby, které vykazují výraznou sociální diferenciaci Současní Ujsini, kterých je okolo 250 000, jsou některými etnology uznáváni za potomky Wu-sunů. Ujsini mají dvě větve Dulat a Sary Ujsin („Žlutí Ujsini). ...
Neurotensin receptor 2
"Expression of neurotensin receptors in human corneal keratocytes". Investigative Ophthalmology & Visual Science. 43 (6): 1765- ...
Ocular immune system
Keratocytes are flattened cells found dispersed within the corneal stroma. The primary role of this sparse population of cells ... It has also been found that the presence of secreted defensins secreted by corneal keratocytes is correlated with cases of ... Corneal nerves serve as a form of defense by detecting the presence of foreign bodies on the corneal surface. This leads to ... Other components of the ocular innate immune system include tears, epithelial cells, keratocytes, corneal nerves, the ...
Badenoch PR, Adams M, Coster DJ (February 1995). "Corneal virulence, cytopathic effect on human keratocytes and genetic ... In cases of corneal ulceration or perforation, or if corneal scarring is severe, corneal transplant may be required. This ... Soft contact lenses are more adherent to the corneal surface than hard lenses, which allows the Acanthamoeba organism to bind ... AK should be considered in all patients who use contact lenses, and following corneal abrasions or trauma. A thorough history ...
Armstrong DJ, Hiscott P, Batterbury M, Kaye S (Jun 2002). "Corneal stromal cells (keratocytes) express thrombospondins 2 and 3 ...
It is produced by corneal keratocytes and is thought to play a role of a dynamic buffer of corneal hydration. In a rare ... Whereas corneal KSI is composed of a number of domains showing variable degrees of sulphation the longest of which may be 8-32 ... Macular dystrophy, corneal, 1 - OMIM Lauder RM, Huckerby TN, Nieduszynski IA (1997). "The structure of the keratan sulphate ... Tai GH, Huckerby TN, Nieduszynski IA (1996). "Multiple non-reducing chain termini isolated from bovine corneal keratan sulfates ...
2007). "Expression of VSX1 in human corneal keratocytes during differentiation into myofibroblasts in response to wound healing ... Mutations in this gene can cause posterior polymorphous corneal dystrophy (PPCD) and keratoconus. Two transcript variants ... 2004). "VSX1 (RINX) mutation with craniofacial anomalies, empty sella, corneal endothelial changes, and abnormal retinal and ...
Stroma of cornea
The lamellæ are produced by keratocytes (corneal connective tissue cells), which occupy about 10% of the substantia propria. ... Macular corneal dystrophy, associated with the loss of keratan sulfate Oyster, CW (1999). "8". The human eye: structure and ... During development of the embryo, the corneal stroma is derived from the neural crest (a source of mesenchyme in the head and ... Branch MJ, Hashmani K, Dhillon P, Jones DR, Dua HS, Hopkinson A (Aug 3, 2012). "Mesenchymal stem cells in the human corneal ...
Prostaglandin EP4 receptor
... corneal endothelium, corneal keratocytes, trabecular cells, ciliary epithelium, conjunctival stromal cells, and iridal stromal ... This result suggests that EP4 activation contributes to corneal neovascularization and that EP4 antagonists may be useful for ... A selective EP4 antagonists significantly reduced corneal neovascularization in rats caused by oxygen-induced retinopathy or ...
Prostaglandin EP3 receptor
... corneal endothelium and keratocytes, trabecular cells, ciliary epithelium, and conjunctival and iridal stroma cells, and ...
In the corneal stroma, keratocytes within the wounded area undergo apoptosis, leaving the stroma devoid of cells that must be ... Keratocytes surrounding the wounded area proliferate and become fibroblasts that migrate to fill the wounded area. This creates ... For example, when corneal integrity is compromised, epithelial cells quickly cover the damaged area by proliferation and ... "Role of Thrombospondin-1 in Repair of Penetrating Corneal Wounds". Investigative Ophthalmology & Visual Science. 54 (9): 6262- ...
Fibroblasts Corneal keratocyte Stem Cells Induced pluripotent stem cell Cluster of differentiation extracellular matrix dermis ... Like corneal fibroblasts, dermal fibroblast proliferation can be stimulated by the presence of fibroblast growth factor (FGF). ... For example, when a dermal fibroblast and a corneal fibroblasts are placed in the same concentrations of fibroblast growth ...
Rabinowitz, YS; McDonnell, PJ (1989). "Computer-assisted corneal topography in keratoconus". Refractive & Corneal Surgery. 5 (6 ... The visible changes in the basal epithelial cell and anterior and posterior keratocytes linked with keratoconus were not ... custom topography-guided transepithelial PRK combined with corneal collagen cross-linking, or corneal transplant. When cross- ... "Post-LASIK ectasia treated with intrastromal corneal ring segments and corneal crosslinking". Digital Journal of Ophthalmology ...
... expressed in the mammalian cornea by the stromal keratocytes and epithelial cells and is reputed to be one of the corneal ...
Corneal pachymetry Corneal reflex Corneal tattooing Corneal topography Eye disease Keratometry List of keratins expressed in ... consisting of regularly arranged collagen fibers along with sparsely distributed interconnected keratocytes, which are the ... For corneal epithelial diseases such as Stevens Johnson Syndrome, persistent corneal ulcer etc., the autologous contralateral ( ... The most common corneal disorders are the following: Corneal abrasion - a medical condition involving the loss of the surface ...
List of distinct cell types in the adult human body
Loose connective tissue fibroblasts Corneal fibroblasts (corneal keratocytes) Tendon fibroblasts Bone marrow reticular tissue ...
... receptor-cells in the skin associated with the sense of touch Corneal keratocytes, specialized fibroblasts residing in the ...
Long-term wear of rigid contacts is associated with decreased corneal keratocyte density and increased number of epithelial ... Wearing lenses designed for daily wear overnight has an increased risk for corneal infections, corneal ulcers and corneal ... corneal edema, descemetocele, corneal ectasia, Mooren's ulcer, anterior corneal dystrophy, and neurotrophic ... "The Corneal Lens", The Optician, 2 September 1949, pp. 141-144. "Corneal Contact Lenses", The Optician, 9 September 1949, p. ...
List of human cell types derived from the germ layers
Satellite glial cell Chromaffin cell Glomus cell Melanocyte Nevus cell Merkel cell Odontoblast Cementoblast Corneal keratocyte ...
Steven E. Wilson
His research has focused on corneal cellular responses to injury and surgery, with his most-cited work being about keratocyte ... He holds two United States patents related to corneal healing. He previously served as a professor and chair of the Department ... CS1 maint: discouraged parameter (link) "Methods and treatments for corneal healing with growth factors". Free Patents Online. ... CS1 maint: discouraged parameter (link) "Methods and treatments for corneal healing with hepatocyte and keratinocyte growth ...
Effects of long-term contact lens wear on the cornea
The number of corneal keratocytes in the epithelial stroma has not been found to change with long-term contact lens wear. ... Increased corneal curvature is yet another change known to arise from long-term contact lens wear; this increase in corneal ... Increases in corneal curvature are thought to be caused by corneal thinning-induced ectasia. Two explanations have been ... Long-term contact lens use can lead to alterations in corneal thickness, stromal thickness, curvature, corneal sensitivity, ...
... the nutrition of the corneal epithelium, stromal keratocytes, and corneal endothelium must occur via diffusion of glucose and ... In addition, excessive corneal hydration can result in edema of the corneal epithelial layer, which creates irregularity at the ... In cases in which irreversible corneal endothelial failure develops, severe corneal edema ensues, and the only effective remedy ... new corneal transplant techniques have been developed to enable more selective replacement of the diseased corneal endothelium ...
Play media Corneal keratocytes (corneal fibroblasts) are specialized fibroblasts residing in the stroma. This corneal layer, ... MACULAR DYSTROPHY, CORNEAL, 1; MCDC1 - OMIM. Patel S, McLaren J, Hodge D, Bourne W (February 2001). "Normal human keratocyte ... Quiescent keratocytes synthesize the so-called crystallins, known primarily for their role in the lens. Corneal crystallins, ... Keratocytes may play a role in different corneal disorders. According to comparative research, their functions drastically ...
Corneal endothelium. *Corneal keratocyte. *Corneal opacity. *Corneal topography. *Corrective lens. *Cover test ...
角膜 - 维基百科，自由的百科全书
Corneal keratocyte（英语：Corneal keratocyte）. *Ocular immune system（英语：Ocular immune system） ... Daxer A, Misof K, Grabner B, Ettl A, Fratzl P. "Collagen fibrils in the human corneal stroma: structure and aging." Invest ... Daxer A, Fratzl P. "Collagen fibril orientation in the human corneal stroma and its implication in keratoconus." Invest ... Facts About The Cornea and Corneal Disease National Eye Institute (NEI). *Keratoconus (Irregular Astigmatism), Patient Support ...
Diferenzas entre revisións de «Queratán sulfato», a enciclopedia libre
Keratocyte phenotype mediates proteoglycan structure: a role for fibroblasts in corneal fibrosis ,journal=[[J. Biol. Chem.]] , ... Nun trastorno progresivo raro chamado [[distrofia corneal macular]], a síntese de queratán sulfato está ausente ou é anormal , ... title = Distribution of proteoglycans antigenically related to corneal keratan sulfate proteoglycan , journal = J. Biol. Chem ... ref name="OMIM_MCDC",[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=217800 Macular dystrophy, corneal, 1] - OMIM,/ref,. ...
Long-term wear of rigid contacts is associated with decreased corneal keratocyte density and increased number of epithelial ... corneal abrasions and erosion, keratitis, corneal edema, descemetocele, corneal ectasis, Mooren's ulcer, anterior corneal ... Wearing lenses designed for daily wear overnight has an increased risk for corneal infections, corneal ulcers and corneal ... "The Corneal Lens", The Optician, 2 September 1949, pp. 141-144. *^ "Corneal Contact Lenses", The Optician, 9 September 1949, p ...
Zhao, M; Agius-Fernandez, A; Forrester, J. V; McCaig, C. D (1996). "Orientation and directed migration of cultured corneal ... "Electrophoresis of Cellular Membrane Components Creates the Directional Cue Guiding Keratocyte Galvanotaxis". Current Biology. ... Zhao, M; Agius-Fernandez, A; Forrester, J. V; McCaig, C. D (1996). "Directed migration of corneal epithelial sheets in ... Pubmed Central reference number: PMC1393020 Klyce, S. D. Electrical profiles in the corneal epithelium. J Physiol 226, 407-429 ...
... corneal transplantation) or corneal transplant. The Boston KPro is a proven primary treatment option for repeat graft failure, ... The holes allow the aqueous humour fluids of the eye to provide nutrients to the donor graft stroma and keratocytes. The device ... corneal transplantation). The Boston KPro is a treatment option for corneal disorders not amenable to standard penetrating ... During implantation of the device, the device is assembled with a donor corneal graft positioned between the front and back ...
Corneal keratocyte - Wikipedia
Play media Corneal keratocytes (corneal fibroblasts) are specialized fibroblasts residing in the stroma. This corneal layer, ... MACULAR DYSTROPHY, CORNEAL, 1; MCDC1 - OMIM. Patel S, McLaren J, Hodge D, Bourne W (February 2001). "Normal human keratocyte ... Quiescent keratocytes synthesize the so-called crystallins, known primarily for their role in the lens. Corneal crystallins, ... Keratocytes may play a role in different corneal disorders. According to comparative research, their functions drastically ...
Keratocytes create stromal spaces to promote corneal neovascularization via MMP13 expression. | Sigma-Aldrich
Keratocytes were the major cells expressing MMP13 in corneal stromas after alkali burns. Through MMP13 expression, keratocytes ... Keratocytes create stromal spaces to promote corneal neovascularization via MMP13 expression.. [Jing Ma, Dan Zhou, Meihua Fan, ... Keratocytes could directly degrade type I collagen and create stromal spaces, promoting corneal neovascularization through ... To investigate the exact mechanism by which keratocytes promote corneal neovascularization. The expression of matrix ...
Derivation of Corneal Keratocyte-Like Cells from Human Induced Pluripotent Stem Cells
... the corneal stroma where they acquired a keratocyte-like morphology and an expression profile similar to corneal keratocytes in ... the embryonic precursor to keratocytes, and then cultured on cadaveric corneal tissue to promote keratocyte differentiation. ... These results indicate that hiPSCs can be used to generate corneal keratocytes in vitro and lay the foundation for using these ... The ability to generate corneal keratocytes in vitro may enable a cell-based therapy to treat patients with keratoconus. Human ...
Expression of Neurotensin Receptors in Human Corneal Keratocytes | IOVS | ARVO Journals
Role of Keratocyte Loss on Corneal Wound Repair after LASIK. In Vivo Gene Delivery and Visualization of Corneal Stromal Cells ... The absence of endogenous NT in corneal keratocytes suggests that NT can be present in corneal nerves or released by nearby ... Keratocyte proliferation studied by MTS assay. Human corneal keratocytes were cultured with various concentrations of JMV449 ( ... Keratocyte proliferation studied by MTS assay. Human corneal keratocytes were cultured with various concentrations of JMV449 ( ...
JFB | Free Full-Text | Fibroblastic Transformation of Corneal Keratocytes by Rac Inhibition is Modulated by Extracellular...
Rabbit corneal keratocytes were plated within standard collagen matrices (2.5 mg/mL) or compressed collagen matrices (~100 mg/ ... biophysical properties modulate corneal keratocyte phenotypes in response to specific wound healing cytokines and Rho GTPases. ... Overall, keratocyte growth factor responses appear to be regulated by both the interplay between Rho and Rac signaling, and the ... Keratocytes within compressed collagen (which has high stiffness and low porosity) maintained a dendritic phenotype following ...
Method for Detecting Corneal Endothelial Cell Identity From Contaminating Keratocytes and Epithelial Cells in Co-Culture | IOVS...
Method for Detecting Corneal Endothelial Cell Identity From Contaminating Keratocytes and Epithelial Cells in Co-Culture ... Method for Detecting Corneal Endothelial Cell Identity From Contaminating Keratocytes and Epithelial Cells in Co-Culture ... Method for Detecting Corneal Endothelial Cell Identity From Contaminating Keratocytes and Epithelial Cells in Co-Culture. ... SMA was expressed in cultured keratocytes but not in whole stromal samples or HCEC. ANGPTL6 and 7 were both expressed in ...
Effects of edible bird's nest (EBN) on cultured rabbit corneal keratocytes | BMC Complementary and Alternative Medicine | Full...
Corneal keratocytes from six New Zealand White Rabbits were isolated and cultured until Passage 1. The proliferative effects of ... This study aimed to investigate the effects of EBN on corneal keratocytes proliferative capacity and phenotypical changes. ... Both phase contrast micrographs and gene expression analysis confirmed the corneal keratocytes retained their phenotypes with ... EBN on corneal keratocytes were determined by MTT assay in serum-containing medium (FDS) and serum-free medium (FD). ...
Automated detection and cell density assessment of keratocytes in the human corneal stroma from ultrahigh resolution optical...
Automated detection and cell density assessment of keratocytes in the human corneal stroma from ultrahigh resolution optical ... Automated detection and cell density assessment of keratocytes in the human corneal stroma from ultrahigh resolution optical ... images of the human corneal stroma is presented. The corneal OCT data is first processed using a state-of-the-art despeckling ... Keratocytes are fibroblast-like cells that maintain the optical clarity and the overall health of the cornea. The ability to ...
Corneal confocal microscopy identifies a reduction in corneal keratocyte density and sub-basal nerves in children with type 1...
Corneal confocal microscopy identifies a reduction in corneal keratocyte density and sub-basal nerves in children with type 1 ... Corneal confocal microscopy identifies a reduction in corneal keratocyte density and sub-basal nerves in children with type 1 ... Corneal sub-basal nerve morphology and corneal keratocyte density (KD) were quantified. ... Results Corneal nerve fibre density (CNFD) (p,0.001), corneal nerve branch density (p,0.001), corneal nerve fibre length (CNFL ...
Corneal confocal microscopy identifies a reduction in corneal keratocyte density and sub-basal nerves in children with type 1...
Corneal confocal microscopy identifies a reduction in corneal keratocyte density and sub-basal nerves in children with type 1 ... Corneal confocal microscopy identifies a reduction in corneal keratocyte density and sub-basal nerves in children with type 1 ... Corneal confocal microscopy identifies a reduction in corneal keratocyte density and sub-basal nerves in children with type 1 ...
Aging | Exosomal miR-19a from adipose-derived stem cells suppresses differentiation of corneal keratocytes into myofibroblasts ...
... on the differentiation of rabbit corneal keratocytes. Keratocytes grown in 10% FBS differentiated into myofibroblasts by ... Thus, exosomal miR-19a derived from the ADSCs suppresses FBS-induced differentiation of rabbit corneal keratocytes into ... HIPK2 enhanced p53 and Smad3 pathways in FBS-induced keratocytes. Keratocytes grown in 10% FBS also showed increased levels of ... Keratocytes treated with 10% FBS and ADSCs-Exo-miR-19a-agomir or ADSCs-Exo-NC-antagomir showed significantly lower levels of ...
Keratocyte loss in corneal infection through apoptosis: a histologic study of 59 cases | BMC Ophthalmology | Full Text
A correlation was attempted between the keratocyte loss, histologic, microbiologic and clinical features. The corneal tissues ... In a study of corneal buttons obtained from patients of corneal ulcer undergoing therapeutic keratoplasty, we observed loss of ... loss of keratocytes in the surrounding stroma (posterior or peripheral). TUNEL positivity was evaluated in the corneal sections ... We report apoptotic cell death of keratocytes in the corneal stroma in infectious keratitis, a phenomenon independent of type ...
Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts<...
Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts. / Karring, Henrik ; ... Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts. I: Investigative ... title = "Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts", ... T1 - Comparison of the water-soluble proteomes from keratocytes and serum-cultured corneal fibroblasts ...
Thy-1 distinguishes human corneal fibroblasts and myofibroblasts from keratocytes - Northumbria Research Link
After corneal injury, keratocytes become activated and transform into repair phenotypes-corneal fibroblasts or myofibroblasts, ... and in keratocytes in normal human corneal tissue sections. Thy-1 mRNA and protein were detectable in cultured human corneal ... Cultured human corneal fibroblasts and myofibroblasts both labelled for Thy-1, but keratocytes in the stroma of normal human ... Human corneal keratocytes were isolated by collagenase digestion. The cells were either processed immediately (i.e. freshly ...
Effects of edible birds nest EBN on cultured rabbit corneal keratocytes - pdf descargar
Effects of edible birds nest EBN on cultured rabbit corneal keratocytes. . Biblioteca virtual para leer y descargar libros, ... Effects of edible birds nest EBN on cultured rabbit corneal keratocytes - Descarga este documento en PDF. Documentación en PDF ... This study aimed to investigate the effects of EBN on corneal keratocytes proliferative capacity and phenotypical changes.. ... Both phase contrast micrographs and gene expression analysis confirmed the corneal keratocytes retained their phenotypes with ...
Keratocyte density in vivo after photorefractive keratectomy in humans
Three months later, keratocyte density was increased in all anteroposterior stromal regions, suggesting that PRK affects ... Keratocyte density was increased in the anterior stroma immediately after PRK in humans. ... Keratocyte density in vivo after photorefractive keratectomy in humans Trans Am Ophthalmol Soc. 1999;97:221-36; discussion 236- ... Conclusions: Keratocyte density was increased in the anterior stroma immediately after PRK in humans. Three months later, ...
Human corneal keratocytes (HCK) are essential for maintaining corneal structure and transparency. This study shows that the ... Blocking TRPV1 activation on keratocytes may therefore be a viable approach to suppress corneal opacification in a clinical ... transient receptor potential vanilloid type 1 activity and myofibroblast transdifferentiation in human corneal keratocytes ...
Cytostatic and cytotoxic effects of 5-fluorouracil on human corneal epithelial cells and keratocytes<...
METHODS: Human corneal epithelial cell (HCEC) and human corneal keratocyte (HCK) cultures were exposed to different 5-FU ... METHODS: Human corneal epithelial cell (HCEC) and human corneal keratocyte (HCK) cultures were exposed to different 5-FU ... METHODS: Human corneal epithelial cell (HCEC) and human corneal keratocyte (HCK) cultures were exposed to different 5-FU ... METHODS: Human corneal epithelial cell (HCEC) and human corneal keratocyte (HCK) cultures were exposed to different 5-FU ...
Gelam honey potentiates ex vivo corneal keratocytes proliferation with desirable phenotype expression | BMC Complementary...
Corneal keratocytes from New Zealand white rabbits were cultured in basal medium (BM) and serum enriched medium (BMS). Serial ... Gelam honey at a concentration of 0.0015 % promotes ex vivo corneal keratocytes proliferation while retaining desirable ... MTT assay was performed on corneal keratocytes in both media to ascertain the optimal dose of GH that produced maximum ... The results serve as a basis for the development of Gelam honey as a potential natural product in promoting corneal wound ...
A cellular and proteomic approach to assess proteins extracted from cryopreserved human amnion in the cultivation of corneal...
Human corneal stromal keratocytes cultured with F-AME or C-AME showed similar morphology and viability, while cell ... can correct early corneal haze in an animal model. Clinical application of cultivated keratocytes is limited by infectious ... Primary human keratocytes from 4 donor corneas were cultured in media supplemented with fresh AME (F-AME) or cryopreserved AME ... It remains unclear if AME from cryopreserved versus fresh human amnion can support human keratocyte propagation, and which ...
Expression of MHC Ⅱ on rat corneal keratocytes is inhibited by special siRNA targeting CⅡTA | Chinese Journal of...
Expression of MHC Ⅱ on rat corneal keratocytes is inhibited by special siRNA targeting CⅡTA / 中国病理生理杂志 ... Five different siRNA were designed, synthesized and transfected into freshly isolated rat corneal keratocytes. At 24 hours ... Expression of MHC Ⅱ on rat corneal keratocytes is inhibited by special siRNA targeting CⅡT ...
DNA Microarray-Based Gene Expression Profiling in Porcine Keratocytes and Corneal Endothelial Cells and Comparative Analysis...
... and G1P3 were highly expressed in porcine keratocytes, compared to porcine corneal endothelial cells or human keratocytes. When ... RNA was isolated from primary cultured porcine or human keratocytes and porcine corneal endothelial cells. Gene expression was ... We, therefore, investigated and compared the gene expression between porcine keratocytes and corneal endothelial cells. ... DNA Microarray-Based Gene Expression Profiling in Porcine Keratocytes and Corneal Endothelial Cells and Comparative Analysis ...
Induction of α-smooth muscle actin expression and myofibroblast transformation in cultured corneal keratocytes<...
... primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate ... primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate ... primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate ... primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate ...
UBIAD1 Mutation Alters a Mitochondrial Prenyltransferase to Cause Schnyder Corneal Dystrophy
Normal human keratocytes were purchased from ScienCell Research Lab (Carlsbad, CA). Schnyder corneal dystrophy and normal human ... Cell lines were established from patients including keratocytes obtained after corneal transplant surgery and lymphoblastoid ... The resultant bilateral corneal opacification is progressive. Approximately 50% of SCD patients have corneal crystalline ... Notation was made as to the presence of central corneal opacity, mid peripheral haze, arcus lipoides and corneal crystalline ...
Journal of Refractive Surgery | September 2005 Issue
Corneal Keratocyte Apoptosis Following Topical Intraoperative Mitomycin C in Rabbits Shu-Wen Chang, MD ... The Effect of Tear Film on Higher Order Corrections Applied to the Corneal Surface During Wavefront-guided Refractive Surgery ... VHF Digital Ultrasound Three-Dimensional Scanning in the Diagnosis of Myopic Regression After Corneal Refractive Surgery Dan Z ...
Forgez P[au] - PubMed - NCBI
In vitro effects of dexamethasone on human corneal keratocytes.. Bourcier T, Borderie V, Forgez P, Lombet A, Rostène W, Laroche ... Expression of neurotensin receptors in human corneal keratocytes.. Bourcier T, Rondeau N, Paquet S, Forgez P, Lombet A, Pouzaud ... Regulation of human corneal epithelial cell proliferation and apoptosis by dexamethasone.. Bourcier T, Forgez P, Borderie V, ...
No relation between the severity of corneal nerve, epithelial, and keratocyte cell morphology with measures of dry eye disease...
keywords = "Corneal confocal microscopy, Corneal epithelial basal cells, Corneal nerves, Dry eye, Keratocytes, Type 1 diabetes ... There was no correlation between DES and TBUT or corneal nerve keratocyte and CEBC morphology. CONCLUSIONS. DES and TBUT are ... There was no correlation between DES and TBUT or corneal nerve keratocyte and CEBC morphology. CONCLUSIONS. DES and TBUT are ... There was no correlation between DES and TBUT or corneal nerve keratocyte and CEBC morphology. CONCLUSIONS. DES and TBUT are ...
POU2F1 Gene - GeneCards | PO2F1 Protein | PO2F1 Antibody
Keratocytes Corneal Stroma * Adult Corneal Superbasal Epithelial Cells Corneal Epithelium * Adult Corneal Basal Epithelial ... Fetal Corneal Superficial Epithelial Cells Corneal Epithelium * ... Corneal Epithelium * Adult Corneal Superbasal Epithelial Cells ... Adult Corneal Superficial Epithelial Cells Corneal Epithelium * Fetal Corneal Superficial Epithelial Cells ... Adult Corneal Superficial Epithelial Cells Corneal Epithelium * ... Corneal Epithelium * Adult Corneal Basal Epithelial Cells ...
AQP5 Gene - GeneCards | AQP5 Protein | AQP5 Antibody
Dr Elena Koudouna - People - Cardiff University
Chick corneal morphogenesis: A new role for keratocytes. Investigative Ophthalmology & Visual Science 2017; 58 (8), 1998. ... Immune Cells on the Corneal Endothelium of an Allogeneic Corneal Transplantation Rabbit Model. Investigative Ophthalmology & ... Chemical composition and sulfur speciation in bulk tissue by x-ray spectroscopy and x-ray microscopy: Corneal development ... X-ray absorption near-edge structure (XANES) spectroscopy identifies differential sulfur speciation in corneal tissue. ...
EpitheliumCorneaEpithelial cellsFibroblastsEndotheliumTissueConfocal microscopyProliferationLoss of cornealHazeCollagenPhenotypePromote cornealExtracellular matrixDystrophyKeratoconusCorneasQuiescentTransplantationCentral corneal thicknessMethodsTransformation of Corneal KeratocytesAbstractThicknessRefractiveRabbitTransparencyExpressed by human keratocytesExcimerLimbusPhenotypesNeovascularizationBlindnessMorphologyEndothelialProgenitor cellsMRNA and proteinUndergo apoptosisProliferative capacityConclusionsMyofibroblast transformationOpacitiesWound HealingKeratan sulfateAutosomal dominantOphthalmologyBiomechanicsPhotorefractive keratectomyTopographyKeratocanGraftStemProteinDebridement
- To protect the eye from injury, the corneal epithelium and the keratocytes of the anterior stroma are provided with a rich nerve supply. (arvojournals.org)
- The purpose of this study was to assess the specificity of protein markers that have previously been identified in corneal endothelium, keratocytes or epithelium. (arvojournals.org)
- Subsequently the epithelium was removed gently with a scalpel and the tissue was cut with a trephine to isolate stromal keratocytes. (arvojournals.org)
- By retaining a flap of corneal epithelium, LASEK may decrease the risk of infection and incidence of corneal haze, while reducing recovery time and postoperative discomfort when compared with PRK. (medscape.com)
- This technique involved the use of alcohol to separate the corneal epithelium from the stroma to create an epithelial sheet that could be repositioned over the ablated stroma. (medscape.com)
- Protein modeling studies revealed an exposed cysteine residue, which might cause interchain disulfide bond formation and protein aggregation leading to disturbed cell junctions of the corneal epithelium. (molvis.org)
- It has been speculated that the loss of function of the gene in corneal tissue results in an increased permeability of corneal epithelium, resulting in the GDLD phenotype. (molvis.org)
- The corneal epithelium accounting for 10% of the central corneal thickness could also contribute to the viscous behaviour. (hindawi.com)
- It is important to bear in mind that the corneal epithelium is easily deformable and is the reference surface for most of the biomechanical corneal measurements. (hindawi.com)
- The epithelium remains smooth until the late stages of the disease, when the subepithelial opacities cause elevations and irregularities of the corneal surface. (harvard.edu)
- The interdependence of the structures integrated into this system and their influence on the corneal epithelium and ultimately on the eyeball makes them of great importance to the health of the eye. (intechopen.com)
- In addition, the primordial cells of the corneal epithelium are located at the corneoscleral limbus. (intechopen.com)
- Green, Colin R. 2018-02-15 00:00:00 In order to maintain a smooth optical surface the corneal epithelium has to continuously renew itself so as to maintain its function as a barrier to fluctuating external surroundings and various environmental insults. (deepdyve.com)
- In the case of deeper corneal injury and corneal pathology the wound healing cascade can also extend to the corneal stroma, the layer below the epithelium. (deepdyve.com)
- EXT(Δ/ΔCEpi) mice (deletion of Ext1 in corneal epithelium) induced at P20 presented progressive thinning of the corneal epithelium with a significant loss in the number of epithelial layers by P55. (sigmaaldrich.com)
- Although no single morphologic feature unique to the disease could be found, the general morphologic pattern of pathology (true keloid formation, an absence of Bowman's layer, subepithelial fibrosis, and abnormal subbasal nerves) likely reflects a novel phenotypic expression of the healing response to recurrent erosion of the corneal epithelium. (diva-portal.org)
- The murine corneal epithelium demonstrated decreased rather than increased cellular layers at the limbal region, in contrast to that of the rat or human epithelium. (ebscohost.com)
- Telomerase expression was widespread throughout many layers of both the murine and rat corneal epithelium. (ebscohost.com)
- In the mouse and rat corneal epithelium P63 expression was maximal in areas of increased proliferative potential. (ebscohost.com)
- In the unperturbed cornea keratocytes stay dormant, coming into action after any kind of injury or inflammation. (wikipedia.org)
- Any glitch in the precisely orchestrated process of healing may cloud the cornea, while excessive keratocyte apoptosis may be a part of the pathological process in the degenerative corneal disorders such as keratoconus, and these considerations prompt the ongoing research into the function of these cells. (wikipedia.org)
- By the end of eye development an interconnected keratocyte network is established in the cornea, with dendrites of neighbouring cells contacting each other. (wikipedia.org)
- After an injury to the cornea, some keratocytes undergo apoptosis, prompted by the signaling molecules secreted by the upper layers, such as IL1 alpha and TNF-alpha. (wikipedia.org)
- In a healthy cornea the programmed cell death is a rare occasion, but immediately after an injury to the uppermost layer keratocytes directly under the injury site commit apoptosis. (wikipedia.org)
- These results indicate that cultured human keratocytes express NTR1 and NTR3 and that NT may exert physiological effects on cornea such as regulation of keratocyte proliferation and apoptosis. (arvojournals.org)
- In addition to their sensory functions, corneal nerves exert important trophic effects on the cornea, including maintenance of epithelial integrity, modulation of cell proliferation and mitosis, stimulation of ion transport, and regulation of wound healing after corneal injuries. (arvojournals.org)
- 2 Furthermore, several studies suggest that substance P and calcitonin gene-related peptide (CGRP) exert important trophic functions on the cornea and promote corneal wound healing. (arvojournals.org)
- Keratocytes are fibroblast-like cells that maintain the optical clarity and the overall health of the cornea. (uwaterloo.ca)
- The ability to measure precisely their density and spatial distribution in the cornea is important for the understanding of corneal healing processes and the diagnostics of some corneal disorders. (uwaterloo.ca)
- The cell density results obtained using the proposed novel approach correlate well with previous work on computerized keratocyte cell counting from confocal microscopy images of human cornea. (uwaterloo.ca)
- Cultured human corneal fibroblasts and myofibroblasts both labelled for Thy-1, but keratocytes in the stroma of normal human cornea did not. (northumbria.ac.uk)
- Three months later, keratocyte density was increased in all anteroposterior stromal regions, suggesting that PRK affects keratocytes throughout the entire central cornea. (nih.gov)
- The corneal stroma, which makes up about 90% of the corneal volume, consists of highly organized extracellular matrix (ECM) interspersed with corneal stromal keratocytes (CSKs), contributing to the physical strength and optical properties of the cornea [ 1 ]. (biomedcentral.com)
- SCD is characterized by an abnormal deposition of cholesterol and phospholipids in the cornea resulting in progressive corneal opacification and visual loss. (pubmedcentralcanada.ca)
- The final stage of the healing processrestores the quiescent state of keratocytes and remodels any disordered extracellular matrix components,leading to a healthy, transparent cornea. (diva-portal.org)
- In the past, the keratocytes of the adult cornea have been characterized as quiescent cells populating the stroma. (nih.gov)
- Keratocytes play an important role in both the healthy and the injured cornea. (nih.gov)
- The formation of corneal haze is an artifact resulting from exposure of the cornea to laser irradiation. (google.com)
- These techniques include photorefractive keratectomy, ("PRK"), phototherapeutic keratectomy ("PTK") in which laser radiation is applied to the cornea with minimal heating effects to ablate or smooth refractive aberrations, and the LASIK procedure which involves slicing a flap from the cornea, directly, laser ablating a portion of the stroma layer, and replacing the corneal flap. (google.com)
- The laser photoablation procedures typically employ a beam shaping or masking apparatus which varies the size of the exposure area on the corneal surface and interior over time or provides a predefined profile of resistance to the laser radiation, such that areas of the cornea receive different cumulative exposures and thereby are ablated to varying depths. (google.com)
- This has brought about a new hypothesis underpinning epithelial differentiation and migration across the cornea with important implications for our current understanding of stem cell niche biology and corneal (limbal) stem cell therapy - of which my co-workers at the Institute of Genetic Medicine have considerable clinical expertise. (ncl.ac.uk)
- Some retrospective studies have shown Peters anomaly to be the most common congenital corneal opacity presenting to a university cornea clinic. (aao.org)
- Keratocytes are cells that work to repair the cornea. (usaeyes.org)
- The concentration and high activity of keratocytes within the cornea after surgery is a contributing factor to late-onset haze. (usaeyes.org)
- All these terms are important because the cornea is considered a viscoelastic material and some devices try to measure and even differentiate between the different components of the biomechanical behavior of the living corneal tissue [ 3 ]. (hindawi.com)
- In the specific case of the human cornea, collagen in Bowman's layer and stroma accounting for over 80% of the dry weight of the cornea would be the major contributor to corneal elasticity. (hindawi.com)
- The new replacement collagen is produced by the resident stromal keratocytes, which respond to injury by undergoing phenotypic transition into fibroblasts and myofibroblasts, as well as by bone-marrow-derived cells that migrate to the cornea in response to injury. (nih.gov)
- The proof-of-concept research, published today in Experimental Eye Research , reports how stem cells (human corneal stromal cells) from a healthy donor cornea were mixed together with alginate and collagen to create a solution that could be printed, a 'bio-ink. (bookofjoe.com)
- PITTSBURGH, Feb. 23, 2015 - Stem cells from the dental pulp of wisdom teeth can be coaxed to turn into cells of the eye's cornea and could one day be used to repair corneal scarring due to infection or injury, according to researchers at the University of Pittsburgh School of Medicine . (upmc.com)
- For the in vivo cornea, the model is used to predict corneal swelling due to increasing intraocular pressure (IOP) and is adapted to model swelling in Fuchs' corneal dystrophy. (royalsocietypublishing.org)
- Identification of keratocyte-like cells differentiated from circulating bone marrow-derived cells in the mouse cornea. (ebscohost.com)
- Matrix metalloproteinase 13 was predominantly expressed by epithelial cells in normal rat corneas, but it was expressed by cells in corneal stromas after alkali burns. (sigmaaldrich.com)
- found that contact with corneal fibroblasts stimulated corneal epithelial cells to produce the matrix metalloprotease MMP9. (sciencemag.org)
- In certain embodiments, the scaffold can be impregnated with one or more keratocytes and a surface of the convex curvature of the scaffold can be covered with one or more layers of epithelial cells. (patents.com)
- and (d) a motor coupled to the fabricated eyelid, wherein the scaffold is impregnated with one or more keratocytes and a surface of the convex curvature of the scaffold is covered with one or more layers of epithelial cells. (patents.com)
- 3. The biomimetic eye model of claim 1, wherein the one or more layers of epithelial cells comprises one or more layers of corneal epithelial cells and one or more layers of conjunctival cells in a concentric circular pattern. (patents.com)
- 4. The biomimetic eye model of claim 3, wherein the one or more layers of the corneal epithelial cells are present within an inner circle of the concentric circular pattern. (patents.com)
- These experiments will entail the characterization of mouse corneal epithelial cells and mouse corneal keratocytes (a specialized fibroblast) using mammalian cell culture techniques. (csbsju.edu)
- Corneal epithelial cells require HS for maintaining corneal homeostasis, and the loss of epithelial HS leads to both impaired wound healing and impaired corneal stratification. (sigmaaldrich.com)
- In addition, as a general finding in all species tested, there was an apparent increase noted in P63 expression in basal corneal epithelial cells in regions that had increased cellular layers (limbus in humans and rats and the paracentral corneal region in the mouse). (ebscohost.com)
- Play media Corneal keratocytes (corneal fibroblasts) are specialized fibroblasts residing in the stroma. (wikipedia.org)
- The activation of fibroblasts from quiescent keratocytes is also observed in cell culture models which show similar phenotypical changes of keratocytes during wounding. (biomedcentral.com)
- After corneal injury, keratocytes become activated and transform into repair phenotypes-corneal fibroblasts or myofibroblasts, however, these important cells are difficult to identify histologically, compromising studies of stromal wound healing. (northumbria.ac.uk)
- Thy-1 mRNA and protein expression by freshly isolated keratocytes and corneal fibroblasts were assessed by RT-PCR and Western blotting. (northumbria.ac.uk)
- Thy-1 was localised immunocytochemically in cultured human corneal fibroblasts, myofibroblasts, and in keratocytes in normal human corneal tissue sections. (northumbria.ac.uk)
- Thy-1 mRNA and protein were detectable in cultured human corneal fibroblasts, but not freshly isolated keratocytes. (northumbria.ac.uk)
- We conclude that Thy-1 expression is induced by transformation of keratocytes to corneal fibroblasts and myofibroblasts, suggesting a potential functional role for Thy-1 in stromal wound healing and providing a surface marker to distinguish the normal keratocyte from its repair phenotypes. (northumbria.ac.uk)
- Using serum and growth factor-supplemented media, CSKs rapidly differentiate into stromal fibroblasts (SFs) and lose specific keratocyte features, including the expression of keratan sulphate proteoglycans (lumican, keratocan), which regulates collagen fibril alignment and spacing, and stromal crystallins (transketolase, aldehyde dehydrogenase ALDH1A1 and 3A1) for transparency and refractivity [ 15 , 16 ]. (biomedcentral.com)
- Under serum-free conditions, with or without bFGF and heparin, primary corneal fibroblasts appeared morphologically similar to in situ corneal keratocytes, demonstrating a broad, stellate morphology with interconnected processes and no α-SM actin expression. (elsevier.com)
- In an earlier study, we showed that human antigen R (HuR) and β-actin expression levels were downregulated in fibroblasts isolated from human keratoconus stroma compared to normal corneal stroma. (molvis.org)
- When the β-actin or HuR gene was individually silenced, the motility and proliferation of corneal fibroblasts were significantly reduced. (molvis.org)
- The results show that downregulation of the HuR gene results in decreased β-actin gene expression, which in turn results in decreased motility and proliferation of corneal fibroblasts. (molvis.org)
- The ground substance, formed mostly by proteoglycans and keratocytes or fibroblasts, would provide the viscous behaviour. (hindawi.com)
- Evaluation for Safety of Cultured Corneal Fibroblasts with Cotreatment of Alcohol and Mitomycin C Investigative Ophthalmology & Visual Science. (jove.com)
- The transparent corneal stroma contains a population of corneal fibroblasts termed keratocytes, which are interspersed between the collagen lamellae. (lu.se)
- However, after corneal injury the keratocytes become activated and transform into backscattering wound-healing fibroblasts resulting in corneal opacification. (lu.se)
- Specifically, corneal haze development is thought to be related to low levels of cytoplasmic enzyme-crystallins in reflective corneal fibroblasts. (lu.se)
- To further investigate this hypothesis, we have used a proteomic approach to identify the most abundant water-soluble proteins in serum-cultured human corneal fibroblasts that represent an in vitro model of the reflective wound-healing keratocyte phenotype. (lu.se)
- This result indicates that wound-healing corneal fibroblasts do not contain enzyme-crystallins. (lu.se)
- Proteins protecting against oxidative stress and protein misfolding were prominent, suggesting that these processes may participate in the generation of cytoplasmic light-scattering from corneal fibroblasts. (lu.se)
- The morphological changes, gene and protein expressions of aldehyde dehydrogenase (ALDH), marker for quiescent keratocytes and vimentin, marker for fibroblasts were detected using q-RTPCR and immunocytochemistry respectively. (blogspot.com)
- Immune Cells on the Corneal Endothelium of an Allogeneic Corneal Transplantation Rabbit Model. (cardiff.ac.uk)
- The HuNu-positive cells transformed into neural crest derivatives such as smooth muscle in cranial blood vessels, stromal keratocytes, and corneal endothelium. (rice.edu)
- Their ability to respond to embryonic cues and generate corneal endothelium and stromal keratocytes provides a basis for understanding the feasibility of creating specialized cells for possible use in regenerative medicine. (rice.edu)
- This brief review examines both basic science and clinical studies to evaluate the potential impact on the health of the corneal endothelium of mitomycin C (MMC) usage during photorefractive keratectomy (PRK). (pubmedcentralcanada.ca)
- 2 Despite the growing popularity of MMC usage during refractive procedures, there has been inconsistency in the conclusions of studies regarding the impact of MMC on the corneal endothelium. (pubmedcentralcanada.ca)
- Because of the non-regenerative nature of the human corneal endothelium, it is imperative to understand how this vital cell layer responds to potential stressors at both the cellular and clinical levels. (pubmedcentralcanada.ca)
- 3 A brief explanation of the mechanism of action of MMC is essential to understand the potential impact of MMC used during PRK on the corneal endothelium. (pubmedcentralcanada.ca)
- Since the endothelium′s importance for corneal function has been known for the last five decades, most research performed on corneal storage media has focused on the preservation of the donor endothelium. (uio.no)
- Other neighbouring keratocytes, when acted upon by the same molecules, become active, proliferate and start synthesizing matrix metalloproteinases that cause tissue remodeling. (wikipedia.org)
- X-ray absorption near-edge structure (XANES) spectroscopy identifies differential sulfur speciation in corneal tissue. (cardiff.ac.uk)
- Chemical composition and sulfur speciation in bulk tissue by x-ray spectroscopy and x-ray microscopy: Corneal development during embryogenesis. (cardiff.ac.uk)
- 2017. Template curvature influences cell alignment to create improved human corneal tissue equivalents . (cardiff.ac.uk)
- Refractive surgery, as it is known today, was not realized until 1966 when Pureskin first appreciated its potential with the demonstration that refractive changes could be made by removing central tissue underneath a corneal flap. (medscape.com)
- These cells are replaced by aggressive stromal cells, which may play an important role in the formation of scar tissue and corneal haze. (nih.gov)
- The lamellæ are produced by keratocytes (corneal connective tissue cells), which occupy about 10% of the substantia propria. (wikipedia.org)
- These techniques involve the use of a pulsed laser photoablation apparatus to ablate very thin layers of corneal tissue with greater precision than can typically be achieved with mechanical means. (google.com)
- My research team seeks to engineer functional replacement and temporary 'bridge' tissues using a modular approach while also developing model systems to study physiological and pathophysiological corneal tissue formation. (ncl.ac.uk)
- LASIK applies the energy underneath a flap of corneal tissue. (usaeyes.org)
- Surface ablation techniques like PRK, and its cousins LASEK and Epi-LASIK, do not use a flap of corneal tissue, but have a higher rate of corneal haze than LASIK. (usaeyes.org)
- Corneal biomechanics is a branch of science that studies deformation and equilibrium of corneal tissue under the application of any force [ 1 ]. (hindawi.com)
- Due to its limitations, a concerted effort has been made by tissue engineers to produce functional, synthetic corneal prostheses as an alternative recourse. (bookofjoe.com)
- We applied this to the area of corneal tissue engineering in order to fabricate corneal structures that resembled the structure of the native human corneal stroma using an existing 3D digital human corneal model and a suitable support structure. (bookofjoe.com)
- The purpose of this experimental study is to evaluate the immediate effect of the CXL treatment on the biomechanical properties and histological microstructure of healthy human corneal tissue. (escrs.org)
- With this purpose, in vitro mechanical tests and histological observation of cross-linked and untreated human corneal tissue were performed. (escrs.org)
- Mechanical tensile test and histological analysis were performed in de-epithelialized healthy human corneal tissue treated with riboflavin solution (0.01% riboflavin-5-phosphate and 20% dextranT-500) - 30 min UVA irradiation (treated sample TS). (escrs.org)
- A similar study was performed to untreated de-epithelialized healthy human corneal tissue (control sample, CS). (escrs.org)
- The treated corneal tissue (TS) resulted 1.8, 1.6, 1.7 and 1.5 fold stiffer than the untreated sample (CS) at 6, 8, 10 and 12% stretch, respectively. (escrs.org)
- The UVA-induced CXL has an immediate biomechanical and histological effect over the treated healthy human corneal tissue. (escrs.org)
- Stiffening of the tissue, keratocyte apoptosis and stromal compacting were observed. (escrs.org)
- The findings, published online today in STEM CELLS Translational Medicine , indicate they also could become a new source of corneal transplant tissue made from the patient's own cells. (upmc.com)
- They also used the cells to develop constructs of corneal stroma akin to natural tissue. (upmc.com)
- Regeneration and fibrosis of corneal tissues Simirskii, V. 2014-09-12 00:00:00 In this review, the features of the regeneration of corneal tissue and its disorders leading to the development of fibrosis are considered. (deepdyve.com)
- The main steps of regeneration of corneal tissues and their disorders that lead to outstripping proliferation of myofibroblasts and secretion of extracellular matrix in the wound area and eventually cause the formation of connective tissue scar and corneal opacity are considered. (deepdyve.com)
- Particular attention is given to the successes of translational medicine in the treatment of corneal tissue fibrosis. (deepdyve.com)
- In 1935, V. P. Filatov was the first to report successful storage of whole eyeglobes at 4°C. The opportunity to store corneal tissue is crucial for extensive use of keratoplasty as treatment for corneal blindness. (uio.no)
- Finally, recent advantages in preservation of cultured corneal/limbal epithelia may increase the availability of this tissue and therefore increase treatment of limbal stem cell deficiency. (uio.no)
- The results also illustrate the structural necessity of fibre inclination in stabilizing the corneal refractive surface with respect to changes in tissue hydration and IOP. (royalsocietypublishing.org)
- The current and projected shortage of transplantable human donor corneas has prompted the development of long-term alternatives to human donor tissue for corneal replacement. (diva-portal.org)
- Burratto's original work involved performing a corrective excimer laser ablation on the back of a resected disc of corneal tissue. (medscape.com)
- Donor corneal graft tissue was obtained from the paracentral region or the anatomical limbal region of murine corneas. (ebscohost.com)
- Methods Twenty participants with T1DM and 20 age-matched healthy controls underwent corneal confocal microscopy. (bmj.com)
- Corneal confocal microscopy was undertaken to quantify corneal nerve fiber density (CNFD), branch density (CNBD), fiber length (CNFL), keratocyte density (KD), and corneal epithelial basal cell (CEBC) density and area. (elsevier.com)
- the corneal form, with intense photophobia and alterations highlighted only with confocal microscopy ( Figure 2 ), and the mixed type which has features of the other three types. (dovepress.com)
- Figure 2 Vernal keratoconjunctivitis (VKC) corneal alterations as observed with confocal microscopy. (dovepress.com)
- In vivo confocal microscopy demonstrated local area of keratocyte loss, with activation of the surrounding keratocytes. (escrs.org)
- By the moment of eye opening after birth the proliferation of keratocytes is all but finished and most of them are in the quiescent state. (wikipedia.org)
- This could be a novel breakthrough as both cell proliferation and functional maintenance are important during corneal wound healing. (biomedcentral.com)
- Cell viability, proliferation and keratocyte marker expression were examined by confocal immunofluorescence and flow cytometry. (biomedcentral.com)
- Shortly, keratocytesadjacent to the corneal wound undergo apoptosis and remaining cells start the process of proliferation andmigration in order to close the wound. (diva-portal.org)
- Moreover,it is hypothesized that the non-neuronal SP and ACh produced by injured keratocytes participate in cornealwound healing by enhancing keratocyte migration and proliferation, and/or by decreasing keratocyteapoptosis. (diva-portal.org)
- The optimal dose of AH in the basal medium (FD) and medium containing serum (FDS) for keratocytes proliferation was identified using MTT assay. (blogspot.com)
- Acetylcholine enhances keratocyte proliferation through muscarinic receptor activation. (umu.se)
Loss of corneal5
- Purpose To assess whether alterations in stromal keratocyte density are related to loss of corneal nerve fibres in children with type 1 diabetes mellitus (T1DM). (bmj.com)
- These changes result in loss of corneal transparency. (nih.gov)
- Stramer B, Fini M.E. (2004) Uncoupling keratocyte loss of corneal crystallin from markers of fibrotic repair. (kcl.ac.uk)
- Corneal transplantation constitutes one of the leading treatments for severe cases of loss of corneal function. (bookofjoe.com)
- Loss of corneal epithelial heparan sulfate leads to corneal degeneration and impaired wound healing. (sigmaaldrich.com)
- Human corneal stromal keratocytes propagated in culture media supplemented with human amnion extract (AME) can correct early corneal haze in an animal model. (biomedcentral.com)
- Our group has previously reported on the use of intrastromal injection of CSKs to arrest corneal haze development and restore corneal clarity in a rat model of early corneal opacities [ 12 ]. (biomedcentral.com)
- This invention relates to the compositions and methods for the treatment of corneal haze. (google.com)
- The compositions and methods are particularly directed to the use of GAG synthesis inhibitors in the treatment of corneal haze. (google.com)
- 1. A method for the treatment of corneal haze which comprises administering to a human patient prior to, during or after corneal laser surgery, or combinations thereof, one or more compositions comprising an effective amount of one or more GAG synthesis inhibitor(s) and a pharmaceutically acceptable vehicle. (google.com)
- Photoablation, however, is followed by a more intense inflammatory response and a greater increase in backscattering of light, commonly referred to as "corneal haze," that is associated with increased keratocyte activation and myofibroblast transformation. (google.com)
- The magnitude of corneal wound repair and the development and duration of corneal haze appears to increase proportionally with in creasing stromal photoablation depth (ice. (google.com)
- Mitomycin C is a medication that changes keratocyte activity and also reduces the probability of late-onset corneal haze in higher myopes and surface ablation correction. (usaeyes.org)
- The LASIK flap virtually eliminates the need for application of Mitomycin C to reduce the probability of corneal haze. (usaeyes.org)
- Equally, the flap reduces the probability of late-onset corneal haze by using a process that limits or reduces the cornea's healing response in a positive and constructive way. (usaeyes.org)
- If you have PRK alone, there is a relatively high probability of having corneal haze. (usaeyes.org)
- This will help reduce the probability of corneal haze with all procedures. (usaeyes.org)
- 1 Since then, MMC usage has expanded significantly within the field of refractive surgery, especially during photorefractive keratectomy (PRK), for prevention of recurrence of corneal haze and as prophylaxis to prevent initial haze development. (pubmedcentralcanada.ca)
- When PRK first became available, its drawbacks included slow reepithelialization, corneal haze and significant postoperative pain as well as delays in the return of vision. (aao.org)
- A corneal haze was first noted at 45 degrees C X 45 minutes X 1 day follow-up. (mendeley.com)
- Once settled in the stroma, keratocytes start synthesizing collagen molecules of different types (I, V, VI) and keratan sulfate. (wikipedia.org)
- Through MMP13 expression, keratocytes directly degraded collagen type I to create stromal spaces, which were convenient for newly formed blood vessels to grow into. (sigmaaldrich.com)
- Expression of MMP13 and collagen type I degradation via keratocytes were induced by VEGFc through VEGFr3 and inhibited by antibodies for VEGFc and VEGFr3. (sigmaaldrich.com)
- Keratocytes could directly degrade type I collagen and create stromal spaces, promoting corneal neovascularization through VEGFc/VEGFr3-induced MMP13 expression. (sigmaaldrich.com)
- Rabbit corneal keratocytes were plated within standard collagen matrices (2.5 mg/mL) or compressed collagen matrices (~100 mg/mL) and cultured in serum-free media, PDGF BB, IGF, FGF2 or TGFβ1, with or without the Rac1 inhibitor NSC23766 and/or the Rho kinase inhibitor Y-27632. (mdpi.com)
- Keratocytes within standard collagen matrices (which are highly compliant) maintained a dendritic phenotype following culture in serum-free media, PDGF, IGF and FGF, but developed stress fibers in TGFβ1. (mdpi.com)
- Keratocytes within compressed collagen (which has high stiffness and low porosity) maintained a dendritic phenotype following culture in serum-free media, PDGF and IGF, but developed stress fibers in both FGF and TGFβ1. (mdpi.com)
- Keratocytes phenotypical changes were morphologically assessed and gene expression of aldehyde dehydrogenase (ALDH), collagen type 1 and lumican were determined through RT-PCR. (biomedcentral.com)
- 2018. Cell regulation of collagen fibril macrostructure during corneal morphogenesis . (cardiff.ac.uk)
- Cloned corneal SP cells under chondrogenic conditions produced matrix staining with toluidine blue and expressed cartilage-specific markers: collagen II, cartilage oligomatrix protein, and aggrecan. (mendeley.com)
- At its centre, human corneal stroma is composed of about 200 flattened lamellæ (layers of collagen fibrils), superimposed one on another. (wikipedia.org)
- Corneal bioprinting utilizing collagen-based bioinks and primary human keratocytes. (ukaachen.de)
- Keratocytes are induced to produce collagen type II: A new strategy for in vivo corneal matrix regeneration. (nih.gov)
- Lumican regulates collagen fibril structure such that gene targeted mice deficient in lumican manifest Ehlers Danlos Syndrome-like skin fragility, tendon weaknesses and corneal opacity. (hopkinsmedicine.org)
- These were 3D bioprinted from an in-house collagen-based bio-ink containing encapsulated corneal keratocytes. (bookofjoe.com)
- The corneal stroma contains unevenly distributed keratocytes and disorganized collagen bundles. (humpath.com)
- The goal of this work is to present a mathematical description of the corneal stroma as an electrolyte gel, characterizing the fluid pressure, swelling behaviour and collagen-swelling interaction. (royalsocietypublishing.org)
- Composite core-and-skirt collagen hydrogels with differential degradation for corneal therapeutic applications. (diva-portal.org)
- Keratocytes create stromal spaces to promote corneal neovascularization via MMP13 expression. (sigmaaldrich.com)
- To investigate the exact mechanism by which keratocytes promote corneal neovascularization. (sigmaaldrich.com)
- It is becoming increasingly clear that keratocytes may play a vital role in regulating the stromal constituents, while also providing structural stability in maintaining the interlamellar organization and, thus, promote corneal transparency. (nih.gov)
- Although significant progress has been made in recent years, pharmaco-therapeutic agents that promote corneal healing remain limited. (deepdyve.com)
- This corneal layer, representing about 85-90% of corneal thickness, is built up from highly regular collagenous lamellae and extracellular matrix components. (wikipedia.org)
- The goal of this study was to investigate how alterations in extracellular matrix (ECM) biophysical properties modulate corneal keratocyte phenotypes in response to specific wound healing cytokines and Rho GTPases. (mdpi.com)
- Keratocytes are the resident cells of the corneal stroma and their function isto produce extracellular matrix components and to take part in corneal healing after injury, which may occurdue to trauma, infection or surgery. (diva-portal.org)
- Keratocytes of the corneal stroma secrete a specialized extracellular matrix essential for vision. (mendeley.com)
- genetic disruption of its synthesis leads to the macular corneal dystrophy. (wikipedia.org)
- According to comparative research, their functions drastically diverge from the norm in keratoconus, the most frequent form of corneal dystrophy. (wikipedia.org)
- Mutations in a novel gene, UBIAD1 , were recently found to cause the autosomal dominant eye disease Schnyder corneal dystrophy (SCD). (pubmedcentralcanada.ca)
- To study the clinical, histological, in vivo confocal microscopic, and molecular profile in a family with gelatinous drop-like corneal dystrophy (GDLD) from north India. (molvis.org)
- Recurrent macular corneal dystrophy following penetrating keratoplasty. (harvard.edu)
- a hypothesis exists that presents excessive keratocyte apoptosis as a major pathological event in keratoconus. (wikipedia.org)
- In most situations, surgical removal (corneal transplantation) is commonly used to restore the vision of patients with opacification, such as in keratoconus, post-infection scarring. (biomedcentral.com)
- Corneal diseases such as keratoconus represent a relatively common disorder in the human population. (harvard.edu)
- However, in recent years, increasing interest has arisen in corneal biomechanics to predict corneal response to surgical or therapeutic interventions and to assist in the detection of early keratoconus [ 4 - 6 ]. (hindawi.com)
- The aim of this study was to compare the pre- and posttreatment corneal densitometry and corneal thickness value of keratoconus (KCN) patients managed via contact lenses (CLs) or by both intrastromal corneal rings and contact lenses. (dovepress.com)
- 3 , 4 Corneal involvement is not common, includes shield ulcers, punctate epithelial keratitis, corneal neovascularization, and can lead to the development of serious complications such as keratoconus or corneal perforation. (dovepress.com)
- Ten patients underwent anterior lamellar keratoplasty using a biosynthetic corneal stromal implant for either advanced keratoconus or central corneal scarring. (diva-portal.org)
- Transplantation of cultured human corneal endothelial cells (HCEC) to treat endothelial dysfunction could help alleviate a worldwide shortage of donor corneas, but a means of definitively proving culture purity must be developed. (arvojournals.org)
- To determine changes in keratocyte density in central human corneas in vivo after photorefractive keratectomy (PRK). (nih.gov)
- Primary human keratocytes from 4 donor corneas were cultured in media supplemented with fresh AME (F-AME) or cryopreserved AME (C-AME). (biomedcentral.com)
- Pallikaris then used the excimer laser ablation on the corneal stromal bed under a hinged flap in rabbit corneas. (medscape.com)
- Stromal keratocytes from normal human corneas were cultured in the presence of serum. (molvis.org)
- Over the past two decades, researchers have developed a variety of techniques that can alter corneal surface for refractive purposes or even for halting disease progression in corneas with mechanical decompensation. (hindawi.com)
- Corneal biomechanics have been assessed in in vitro studies by measuring stress-strain and Young's modulus in isolated corneas [ 8 ]. (hindawi.com)
- Yet there is a significant shortage of corneas available to transplant, with 10 million people worldwide requiring surgery to prevent corneal blindness as a result of diseases such as trachoma, an infectious eye disorder. (bookofjoe.com)
- Corneal blindness, which affects millions of people worldwide, is typically treated with transplants of donor corneas, said senior investigator James Funderburgh, Ph.D., professor of ophthalmology at Pitt and associate director of the Louis J. Fox Center for Vision Restoration of UPMC and the University of Pittsburgh , a joint program of UPMC Eye Center and the McGowan Institute for Regenerative Medicine. (upmc.com)
- The team injected the engineered keratocytes into the corneas of healthy mice, where they integrated without signs of rejection. (upmc.com)
- PRK is especially useful in patients with thin corneas, large pupils, corneal scars, epithelial basement membrane disease or a his- tory of radial keratotomy, channel IOLs or corneal transplants. (aao.org)
- Corneal astigmatism and surface steepness were observed 4 years after BSS implantation, while the implants remained stably integrated in the host corneas. (diva-portal.org)
- Quiescent keratocytes synthesize the so-called crystallins, known primarily for their role in the lens. (wikipedia.org)
- Apoptosis of keratocytes, either in quiescent or active state, is a process that attracts special attention. (wikipedia.org)
- Under normal conditions, the keratocytes are quiescent and transparent. (lu.se)
- However, treatment is restricted to corneal transplantation, which only occurs in the most advanced cases. (harvard.edu)
- The biosynthetic stromal substitutes (BSS) characterized herein represent a potentially safe alternative to donor organ transplantation for anterior corneal stromal diseases. (diva-portal.org)
- Future studies will indicate if biomaterials technology will allow for the optimization of postoperative surface irregularity after anterior stromal replacement, a new window of opportunity that is not available with traditional corneal transplantation techniques. (diva-portal.org)
Central corneal thickness2
- Corneal thickness was found to be statistically significantly different between pre treatment and post treatment for the CL group for central corneal thickness (CCT) and thinnest area ( P =0.01 and P =0.02), respectively. (dovepress.com)
- Average central corneal thickness is about 550 µm, increasing to about 700 µm in the periphery. (medscape.com)
- METHODS: Human corneal epithelial cell (HCEC) and human corneal keratocyte (HCK) cultures were exposed to different 5-FU concentrations (0.025%-1%) and incubation durations (5 minutes to 2 hours). (elsevier.com)
- The methods of cell therapy aimed at the restoration of stem cell pool of corneal tissues are the most promising. (deepdyve.com)
- Methods and analysis Primary human cells of corneal surface origin were obtained from eye bank eyes. (bmj.com)
Transformation of Corneal Keratocytes1
- Addition of TGF(β1) to serum-free cultures resulted in a dramatic transformation of corneal keratocytes to spindle-shaped, fibroblast-like cells that expressed α-SM actin in 100% of cells and exhibited a 20-fold increase in fibronectin synthesis and a 13-fold increase in α 5 β 1 -integrin synthesis. (elsevier.com)
- abstract = "PURPOSE: To investigate the effects of various 5-fluorouracil (5-FU) concentrations, exposure times, and application techniques on in vitro-cultured human corneal cells. (elsevier.com)
- Since its introduction, LASIK has been associated with various complications, specifically when performed on eyes with decreased corneal thickness, irregular astigmatism, dryness, preexisting ocular surface disease, or glaucoma, to the point where several of these entities have become relative contraindications to performing LASIK. (medscape.com)
- Data of corneal densitometry and corneal thickness were collected using the Oculus Pentacam at the pretreatment visit and posttreatment visit at 12 months. (dovepress.com)
- In addition to these experiments, I will be performing experiments to determine the role of Ctsc (a protein implicated in contributing to corneal thickness) in mouse corneal cells. (csbsju.edu)
- Anterior OCT demonstrated structural damage of the half of corneal thickness. (escrs.org)
- Concepts of corneal refractive surgery, such as keratectomy, keratotomy, and thermokeratoplasty, were first described in 1898 by Lans who published a set of experiments that focused on treating astigmatism in rabbits. (medscape.com)
- It appears that the improved outcomes of laser refractive procedures will--to a large extent--depend on our ability to maintain keratocyte health, while also controlling the negative effects of cells replacing injured or dead keratocytes. (nih.gov)
- The surgical technique involves the creation of a hinged lamellar corneal flap, after which an excimer laser is used to make a refractive cut on the underlying stromal bed. (medscape.com)
- Jose Barraquer is generally credited with much of the early work leading to corneal lamellar refractive procedures as they are currently practiced. (medscape.com)
- Downregulation of HIPK2 suppresses FBS-induced differentiation of rabbit corneal keratocytes into myofibroblasts. (aging-us.com)
- A ) Histogram plot shows HIPK2 kinase activity in rabbit corneal keratocytes grown in DMEM/F12 medium with and without 10% FBS. (aging-us.com)
- B , C ) Western blot analysis shows levels of HIPK2, Smad3, p-Smad3, p53, and p-p53 in rabbit corneal keratocytes grown in DMEM/F12 medium with and without 10% FBS. (aging-us.com)
- D ) QRT-PCR analysis shows relative HIPK2 mRNA levels in rabbit corneal keratocytes transfected with NC-siRNA, HIPK2-siRNA1, HIPK2-siRNA2, and HIPK2-siRNA3 for 48 h. β-actin mRNA levels were used for normalization. (aging-us.com)
- E ) HIPK2 kinase activity in rabbit corneal keratocytes, transfected with NC-siRNA or HIPK2-siRNA3, and grown in DMEM/F12 medium with 10% FBS for 48 h. (aging-us.com)
- F , G ) Western blot analysis shows HIPK2, Smad3, p-Smad3, p53, and p-p53 levels in rabbit corneal keratocytes, transfected with NC-siRNA or HIPK2-siRNA3, and grown in DMEM/F12 medium with 10% FBS for 48 h. (aging-us.com)
- Primary rabbit corneal keratocytes were grown under serum-free conditions or in media supplemented with serum (10% fetal calf serum), TGF(β1) (0.1-10 ng/ml), basic fibroblast growth factor (bFGF) (0.1-10 ng/ml), or heparin (10 U/ml). (elsevier.com)
- Pubmed ID: 12714623 The purpose of this study was to quantify the effect of mitomycin C on rabbit keratocytes, with a view to determining its potential in modulating corneal stromal wound healing. (jove.com)
- Pubmed ID: 14691158 To investigate the effects of alcohol and mitomycin C (MMC) on cultured corneal fibroblast of the rabbit to determine the safety of this compound for clinical use. (jove.com)
- Corneal crystallins, like the lens ones, are thought to help maintain the transparency and optimal refraction. (wikipedia.org)
- These homeostatic characteristics are important for corneal transparency. (biomedcentral.com)
- 2012. Corneal myofibroblast biology and pathobiology: Generation, persistence, and transparency. (nih.gov)
- At present, the most popular hypothesis suggests that particular abundant water-soluble proteins called enzyme-crystallins are involved in maintaining corneal cellular transparency. (lu.se)
Expressed by human keratocytes1
- Crystallins expressed by human keratocytes are ALDH1A1, ALDH3A1, ALDH2 and TKT. (wikipedia.org)
- Buratto performed excimer laser ablation on the posterior surface of the resected corneal disc before replacing and resuturing it back to its original position. (medscape.com)
- External corneal injury, such as epithelial debridement and excimer laser exposure, can cause profound anterior keratocyte loss. (nih.gov)
- Excimer laser transepithelial photoablation induces significantly less keratocyte loss than manual epithelial debridement. (google.com)
- 3) Wu WCS, Stark W, Green WR, "Corneal wound healing after 193-nm excimer laser keratectomy. (harvard.edu)
- Pallikaris developed the technique of performing the excimer laser corrective ablation in the corneal stromal bed under a hinged flap. (medscape.com)
- Near the corneal limbus, stromal cells expressed ABCG2, a protein present in many adult stem cells. (mendeley.com)
- Both phase contrast micrographs and gene expression analysis confirmed the corneal keratocytes retained their phenotypes with the addition of EBN. (biomedcentral.com)
- The cells were either processed immediately (i.e. freshly isolated keratocytes) or were cultured in the presence of 10% fetal bovine serum or transforming growth factor-β to induce transformation to the corneal fibroblast and myofibroblast phenotypes, respectively. (northumbria.ac.uk)
- 2006. The keratocyte: Corneal stromal cell with variable repair phenotypes. (nih.gov)
- Corneal neovascularization was observed under a slit lamp microscope and evaluated via immunohistochemistry. (sigmaaldrich.com)
- Despite extensiveresearch on corneal wound healing, corneal scarring remains a major cause of preventable blindness. (diva-portal.org)
- A study of congenital eye disease at a teaching hospital in Sagamu, Nigeria, found that corneal opacities is the fourth most common congenital eye disorder causing blindness in 5.7% of infants with congenital eye diseases (Bodunde et al, 2006). (aao.org)
- In addition, almost 5 million people suffer total blindness due to corneal scarring caused by burns, lacerations, abrasion, or disease. (bookofjoe.com)
- New research by bioengineer David Schmidtke is helping understand how corneal repair cells called keratocytes sometimes result in scarring and blindness. (nih.gov)
- Corneal sub-basal nerve morphology and corneal keratocyte density (KD) were quantified. (bmj.com)
- Corneal keratocytes grown in the presence of serum showed a typical fibroblast morphology with induction of α-SM actin expression in 1 to 10% of cells. (elsevier.com)
- There was no correlation between DES and TBUT or corneal nerve keratocyte and CEBC morphology. (elsevier.com)
- DES and TBUT are significantly increased in patients with T1DM, but are not related to corneal nerve, basal epithelial, or keratocyte cell morphology. (elsevier.com)
- This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. (blogspot.com)
- Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. (blogspot.com)
- Porcine to rat corneal xenotransplantation resulted in severe inflammation and rejection of the corneal stroma , whereas an allograft showed mainly endothelial cell -associated rejection. (bvsalud.org)
- We, therefore, investigated and compared the gene expression between porcine keratocytes and corneal endothelial cells . (bvsalud.org)
- RNA was isolated from primary cultured porcine or human keratocytes and porcine corneal endothelial cells . (bvsalud.org)
- Real-time polymerase chain reaction ( PCR ) was performed for C1R, CCL2, CXCL6, and HLA-A in porcine keratocytes and corneal endothelial cells . (bvsalud.org)
- As a result, upregulated expression more than 2 folds was observed in 1,162 genes of porcine keratocytes versus porcine endothelial cells . (bvsalud.org)
- When measured by real-time PCR , the expression of C1R, CCL2, and HLA-A was higher in porcine keratocytes compared to that in porcine corneal endothelial cells . (bvsalud.org)
- Higher thermal doses produced a spectrum of damage, with complete destruction of all keratocytes and endothelial cells at 59 degrees C X 45 min. (mendeley.com)
- This study sought to identify progenitor cells for human keratocytes. (mendeley.com)
MRNA and protein2
- Some keratocytes underlying the site of injury, even a light one, undergo apoptosis immediately after the injury. (wikipedia.org)
- This study aimed to investigate the effects of EBN on corneal keratocytes proliferative capacity and phenotypical changes. (biomedcentral.com)
- CONCLUSIONS: Coadministration of TTX and PPC significantly prolonged corneal anesthesia, but in view of delayed corneal reepithelialization, caution is suggested in the use of the drug combination. (biomedsearch.com)
- In addition, the growth of keratocytes in serum appears to mimic, in part, in vivo activation and myofibroblast transformation. (elsevier.com)
- The prevalence of congenital corneal opacities (CCO) is estimated to be 3 in100,000 newborns. (aao.org)
- Table 2 highlights the differential diagnosis of infantile corneal opacities and the method of diagnosis. (aao.org)
- The aim of this study was to describe morphological changes in a new corneal disease, Dystrophia Smolandiensis, characterized by recurrent corneal erosive episodes and formation of central corneal keloid-like opacities in approximately half of those affected. (diva-portal.org)
- There has been no effective treatment or agent that is available for corneal injury in promoting corneal wound healing. (biomedcentral.com)
- The process of corneal wound healing is complex. (diva-portal.org)
- However, when the process of corneal wound healing is incompleteor disturbed, corneal scarring may occur, which can lead to significantly impaired vision. (diva-portal.org)
- Further analysis of these cells will aid elucidation of molecular mechanisms of corneal development, differentiation, and wound healing. (mendeley.com)
- This ability to communicate appears instrumental in triggering and orchestrating the corneal response in wound healing. (nih.gov)
- To further extend the finding, we determined whether HuR expression affects β-actin gene expression and in turn affects corneal fibroblast migration and wound healing. (molvis.org)
- We conclude that decreased β-actin expression in normal corneal stroma clearly disrupts the cytoskeletal structure and functions, including keratocyte motility and wound healing. (molvis.org)
- Mitomycin C (MMC) was proposed to be an effective modulator of the wound healing response after corneal stromal ablation as early as 1991. (pubmedcentralcanada.ca)
- Acacia honey is a natural product which has proven to have therapeutic effects on skin wound healing, but its potential healing effects in corneal wound healing have not been studied. (blogspot.com)
- The results of the present study show promising role of AH role in accelerating the initial stage of corneal wound healing. (blogspot.com)
- Minns MS, Trinkaus-Randall V. Purinergic Signaling in Corneal Wound Healing: A Tale of 2 Receptors. (bu.edu)
- This study aimed to elucidate the role of HS in corneal epithelial homeostasis and wound healing. (sigmaaldrich.com)
- The conditional knockout of HS from keratocytes using the keratocan promoter led to no corneal abnormalities or any disruption in wound healing. (sigmaaldrich.com)
- Cloned SP cells cultured in medium with fibroblast growth factor-2 lost ABCG2 and PAX6 expression and upregulated several molecular markers of keratocytes, including keratocan, aldehyde dehydrogenase 3A1, and keratan sulfate. (mendeley.com)
- They are thought to accumulate because of an inability to break down corneal keratan sulfate. (harvard.edu)
- an autosomal dominant disorder characterized by numerous small white flecks present in all layers of the corneal stroma. (bio-rad.com)
- The main aim of this thesis was to investigate two hereditary corneal diseases with recurrent erosions in order to find out if they had been described before, and more specifically to describe the clinical picture and the morphological changes, differentiate them from other known autosomal dominant corneal dystrophies with a clinical resemblance, and to exclude genetic linkage to known corneal dystrophies with autosomal-dominant inheritance and a clinical resemblance. (diva-portal.org)
- Apoptosis in Keratocytes Caused by Mitomycin C Investigative Ophthalmology & Visual Science. (jove.com)
- Experiments conducted by lead author Fatima Syed-Picard, Ph.D., also of Pitt's Department of Ophthalmology, and the team showed that stem cells of the dental pulp, obtained from routine human third molar, or wisdom tooth, extractions performed at Pitt's School of Dental Medicine, could be turned into corneal stromal cells called keratocytes, which have the same embryonic origin. (upmc.com)
- Other experts question whether patients with problematic corneal shapes should receive laser vision correction at all, said Marguerite B. McDonald, MD, clinical professor of ophthalmology at New York University. (aao.org)
- Additionally, increasing interest has also arisen in corneal biomechanical properties and glaucoma once corneal biomechanics have been shown to influence intraocular pressure (IOP) measurements and may be also indicative of ocular globe biomechanics that could also be predictive of glaucoma susceptibility [ 7 ]. (hindawi.com)
- We show that such a model can extend predictive accuracy in corneal biomechanics. (royalsocietypublishing.org)
- However, as currently practiced, it is perhaps best thought of as photorefractive keratectomy (PRK) performed under a flap instead of on the corneal surface. (medscape.com)
- Corneal topography revealed 8 diopters irregular astigmatism. (escrs.org)
- The aims of this thesis project were to test these hypotheses and to study the underlying inter- andintracellular mechanisms of the effects of SP and ACh on keratocytes.Results: Cultured primary cells of the human corneal stroma expressed keratocyte markers (keratocan,lumican, CD34, and ALDH), the tachykinins SP and NKA, catecholamines (adrenaline, noradrenaline anddopamine), ACh, and glutamate. (diva-portal.org)
- Specimens of one primary corneal graft, one regraft, and one biopsied keloid-like region, obtained from members of a large family with the disease, were re-examined with a light microscope, and sections were stained with Congo red and immunohistochemically analyzed for fibronectin and S100A4. (diva-portal.org)
- The aim of this paper was to develop a GFP-expressing transgenic mouse model for the keratoepithelioplasty and to use this to follow the outcome of this form of graft, when placed on an inflamed corneal surface. (ebscohost.com)
- These results demonstrate the presence of a population of cells in the human corneal stroma expressing stem cell markers and exhibiting multipotent differentiation potential. (mendeley.com)
- During development of the embryo, the corneal stroma is derived from the neural crest (a source of mesenchyme in the head and neck  ) which has been shown to contain mesenchymal stem cells . (wikipedia.org)
- Angiopoietin like protein 7 (ANGPTL7) has been determined to be specific to the corneal stroma so we tested its specificity on cultured cells along with the expression of other members of the angiopoietin like family (ANPTL1-6). (arvojournals.org)
- It is feasible to use protein extract from cryopreserved amnion to propagate human keratocytes for potential translational applications. (biomedcentral.com)
- Immunohistochemistry using antibodies specific for UBIAD1 protein in keratocytes revealed that both wild type and N102S protein were localized sub-cellularly to mitochondria. (pubmedcentralcanada.ca)
- Densitometry of one-dimensional gels revealed that no single protein isoform exceeded 5% of the total water-soluble protein fraction, which is the qualifying property of a corneal enzyme-crystallin according to the current definition. (lu.se)
- The effect of test compounds on the rate of corneal epithelialization was studied in vivo after corneal debridement. (biomedsearch.com)