Corneal Keratocytes

Corneal Stroma

Cornea

Fibroblasts

Rabbits

Wound Healing

Cells, Cultured

Fluorescent Antibody Technique, Indirect

Cell Culture Techniques

Actins

RNA, Messenger

Blotting, Western

Keratan Sulfate

Epithelium, Corneal

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Human Fetal Keratocytes Have Multipotent Characteristics in the Developing Avian Embryo

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CIL:12013, Hypsophrys nicaraguensis, keratocyte. CIL. Dataset

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Paragon Genomics

Congenital corneal anaesthesia and the MURCS association: a case report. | British Journal of Ophthalmology

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Omega Research

Keratan sulphate is unevenly distributed from back to front of bovine cornea | Biochemical Society Transactions

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Fixing and staining tissue culture cells or slices grown in a 3D collagen matrix with fluorescent phalloidin

CIL:35062. CIL. Dataset

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Literatur zu EbN / EbM

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Wyniki wyszukiwania

An unusual presentation of diabetic neuropathy | British Journal of Ophthalmology

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WinSanTor

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Wusunové - Wikipedie

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Corneal keratocyte

Neurotensin receptor 2

Ocular immune system

Acanthamoeba keratitis

Thrombospondin-2

Thrombospondin 3

Keratan sulfate

VSX1

Stroma of cornea

Prostaglandin EP4 receptor

Prostaglandin EP3 receptor

Haptotaxis

Dermal fibroblast

Post-LASIK ectasia

Transketolase

Cornea

Corneal endothelium

List of distinct cell types in the adult human body

Dendrite (non-neuronal)

Contact lens

List of human cell types derived from the germ layers

Steven E. Wilson

Effects of long-term contact lens wear on the cornea

Bioelectricity

Boston keratoprosthesis

Mooren's ulcer

Cell culture

Stroma22

• Extracellular fibrillogranular material was present in the corneal stroma, Descemet's membrane, and scleral stroma. (elsevier.com)
• Human Keratocytes, or corneal fibroblasts, are fibroblasts residing in the corneal stroma. (innoprot.com)
• Fibroblasts which occur in the CORNEAL STROMA . (bvsalud.org)
• Erie reported altered keratocyte morphology and density within the anterior stroma in contact lens wearers with keratoconus. (optometrytimes.com)
• Keratocytes from the corneal stroma of six New Zealand white rabbits were isolated and cultured until passage 1. (blogspot.com)
• Being a small protein roughly the size of albumin, it has the capability to diffuse through the corneal stroma. (eyerounds.org)
• The stroma consisted of lamellae of different thickness with few keratocytes. (unipa.it)
• The stroma was thinner in the conjunctiva than in the cornea, while corneal lamellae were thicker in the intermediate stroma. (unipa.it)
• Macular corneal dystrophy is a rare inherited disease of the cornea leading to deposits mainly in the stroma. (biomedcentral.com)
• Slit-lamp examination revealed multiple dense, poorly circumscribed grey-white patchy changes in the stroma accompanied by corneal opacity in both eyes. (biomedcentral.com)
• The AMP staining demonstrated blue deposits typical of macular corneal dystrophy, mainly in the stroma but also in the endothelium. (biomedcentral.com)
• Mimicking Corneal Stroma Using Keratocyte Loaded Photopolymerizable Methacrylated Gelatin Hydrogels. (metu.edu.tr)
• The result, in less than 10 minutes, was a corneal structure "that resembled the structure of the native human corneal stroma," the study said. (reviewofoptometry.com)
• 3D bioprinting of a corneal stroma equivalent. (reviewofoptometry.com)
• In keratoconus, the corneal elastic modulus is reduced due to pathology in the corneal stroma. (ijkecd.com)
• Normal keratocan expression in adult tissues is limited to the corneal stroma and is considered. (mdbioproducts.com)
• In keratocan knockout mice (Kera-/-) the corneal stroma is thinner, the cornea-iris angles are narrower and the collagen fibers of the corneal stroma are disorganized when compared to wild-type animals. (mdbioproducts.com)
• To enable this, the density of keratocytes in the avian corneal stroma is less and the collagen bundles are better arranged. (histology-of-birds.com)
• In this study, the mechanical properties of corneal stroma models were quantitatively monitored by a vibrational optical coherence elastography (OCE) system during continuous culture periods. (keele.ac.uk)
• This study demonstrates that the vibrational OCE system is capable of quantitative monitoring the changes in mechanical properties of a corneal stroma wound model during continuous culture periods and improves our understanding on corneal wound healing processes. (keele.ac.uk)
• One hour and corneal stroma heals, you would only viable option, analgesics may not our testimonials in infective keratitis? (farideh.ca)
• These extend into the developing cornea from the epithelial basal lamina and appear to associate with the neural crest cells that migrate inwardly to form, the corneal endothelium and then keratocytes which synthesise the mature initial, supplementary corneal stroma. (phytid.org)

Epithelium9

• The integrity of the corneal surface epithelium is essential for maintaining clarity of vision. (nottingham.ac.uk)
• Most importantly we isolated a population of corneal stromal multipotent stem cells that have the ability to produce corneal epithelium, and are investigating their role in vivo corneal regeneration. (nottingham.ac.uk)
• TGFβI is located on chromosome 5q31 and codes for keratoepithelin, a protein secreted by corneal epithelium. (eyerounds.org)
• The corneal clouding is due to intracellular accumulations of mucopolysaccharides in corneal epithelium and keratan sulfate in keratocytes. (arizona.edu)
• Results: The corneal epithelium was formed by five layers of cells. (unipa.it)
• Alkalis erode through the corneal epithelium and penetrate into the anterior chamber. (emdocs.net)
• Interestingly, D6 was highly expressed in CD45 -: cells and the corneal epithelium of accepted corneal allografts. (harvard.edu)
• The earliest sign of active viral replication in the corneal epithelium is the development of small, raised, clear vesicles that are analogous to the vesicular eruptions seen in mucocutaneous herpes infection elsewhere in the body. (medscape.com)
• Prominent features of a dendritic ulcer include a linear branching pattern within the corneal epithelium with terminal bulbs, swollen epithelial borders that contain live viruses, and central ulceration through the basement membrane. (medscape.com)

Cornea18

• This includes a doctoral thesis on corneal innervation in dogs and cats (2003), followed by a post-doc establishing in vivo confocal microscopy of the cornea in veterinary ophthalmology at the Ludwig-Maximilian University of Munich in cooperation with a private ophthalmology referral clinic (2003-2005), followed by an ocular pathology fellowship (2008, Comparative Ocular Pathology Laboratories of Wisconsin, Wisconsin University). (rvc.ac.uk)
• Dr. Reidy is a recognized expert in the field of corneal surgery, and has recently completed a multicentered trial on cornea collagen cross-linking for the treatment of keratoconus. (doximity.com)
• Leaving a narrow hinge of tissue allowed the outer cornea to become a flap that is reflected out of the way during the laser exposure, then returned to its original position and allowed to adhere through natural corneal dehydration to the underlying stromal bed (Figure 1). (aao.org)
• Specifically, the use of decellularised cornea scaffolds is explored to look at the effects of mechanical stiffness in determining the fate of corneal limbal stem cells when they repopulate the acellular scaffolds. (bl.uk)
• The greatest challenge in developing a bio-engineered cornea has always been to recapitulate the corneal stromal biology within the artificial scaffold. (bl.uk)
• Human Keratocytes (HK), aka Corneal Fibroblasts, provided by Innoprot are isolated from human healthy cornea. (innoprot.com)
• Under normal conditions, the keratocytes in the adult cornea are relatively quiescent cells. (innoprot.com)
• Keratocytes express functional IL-4Rs and IL-17R on the cell surface, suggesting that these cells may contribute to the role of IL-4 and IL-17 as mediators of allergic reactions in the cornea. (innoprot.com)
• Keratoconus is described as a bilateral but asymmetric, noninflammatory, progressive degeneration of the cornea, leading to extreme refractive errors with impaired visual acuity and conical corneal protrusion. (optometrytimes.com)
• Corneal epithelial-stromal and stromal dystrophies are a group of inherited disorders of the cornea that are caused by progressive accumulation of deposits within the layers of the cornea. (eyerounds.org)
• Corneal dystrophies are rare, inherited disorders of the cornea which can affect different layers of the cornea. (biomedcentral.com)
• 3 - 5 The collagen cross-linking (CXL) treatment introduced by Wollensak et al 6 in 2003 was accompanied by a reduction of a lesser degree compared to ICRS of maximal keratometry (2.01 D) and refractive error (1.14 D). It also increased corneal rigidity by 4.5 times and seemed to be an effective mean for stabilizing the cornea. (ijkecd.com)
• The CXL is a procedure used for stabilizing the cornea in patients with progressive keratoconus by increasing corneal rigidity, but without major refractive and visual changes. (ijkecd.com)
• Corneal wound healing, caused by frequent traumatic injury to the cornea and increasing numbers of refractive surgeries, has become a vital clinical problem. (keele.ac.uk)
• Collagen-based hydrogels incorporating corneal cells are suitable for replicating a three-dimensional (3D) equivalent of the cornea in-vitro. (keele.ac.uk)
• CONCLUSIONS: Although the m-KPro device augments the inflammatory response in the cornea after its implantation, allogenicity (of the carrier tissue) is also a significant contributor to corneal inflammation. (harvard.edu)
• Although corneal allotransplantation is performed in the immune-privileged cornea, many grafts are still rejected after transplantation. (harvard.edu)
• The effect of donor age on corneal transplantation outcome results of the Cornea Donor Study. (jamanetwork.com)

Tissue16

• With 39 million corneal blind worldwide, developing affordable technologies as replacement tissue for corneal regeneration is a leading healthcare and therefore scientific priority. (nottingham.ac.uk)
• Second, use of a cryolathe to freeze the corneal cap for precise lenticular reshaping of corneal tissue. (aao.org)
• In the mid-1980s, Swinger, Krumeich, and Cassiday developed a technique for removing corneal tissue without the cryolathe. (aao.org)
• In the late 1980s, Ruiz and Rowsey introduced tissue removal from the stromal bed rather than the free corneal cap, referred to as in situ keratomileusis. (aao.org)
• The microkeratome was removed the corneal cap in a first pass, then the suction ring was changed to excise a free lenticle of tissue with a second pass. (aao.org)
• This work therefore demonstrates the influence of mechanical and chemical conditionings on corneal cells function and may provide a greater understanding in the study of corneal biology and tissue engineering application. (bl.uk)
• ALDH enzyme activity was also quantitated and immunolabeling was performed to determine the expression of ALDH3A1 in human corneal tissue sections from normal and diseased corneas. (northumbria.ac.uk)
• Survey of Ophthalmology Induction of cross-links in corneal tissue Spörl, E. (kcglobal.org)
• Honey has very effective wound-healing properties and its use with delicate corneal tissue is a very important discovery. (blogspot.com)
• Corneal stromal stem cells (CSSC) reduce corneal inflammation, prevent fibrotic scarring, and regenerate transparent stromal tissue in injured corneas. (biomedcentral.com)
• This study has demonstrated that the corneal regenerative effect of hCSSC is mediated by TGFβ3, inducing a scar-free tissue response. (biomedcentral.com)
• The underlying mechanisms of how CSSC reduce corneal fibrosis and scarring, as well as regenerate stromal tissue, have yet to be elucidated. (biomedcentral.com)
• This is the first case report describing the clinical and histopathological findings of corneal tissue after failed phototherapeutic keratectomy (PTK) in a patient with macular corneal dystrophy. (biomedcentral.com)
• 1 Conventional replacement of corneal tissue is limited by the scarcity of human donor grafts relative to need. (reviewofoptometry.com)
• Present research is focused on using adult stem cells to regenerate tissue and corneal transparency within knockout mice. (mdbioproducts.com)
• These data suggest that using syngeneic or decellularized corneal tissue as a Boston-KPro carrier could reduce the postoperative inflammation response. (harvard.edu)

Collagen8

• Keratocyte Apoptosis After Corneal Collagen Cross-linking Using Riboflavin/UVA Treatment Wollensak, G. (kcglobal.org)
• Summary: Mixing together human corneal stromal cells with alginate and collagen to make a bioink, researchers have successfully created 3D printed human corneas. (neurosciencenews.com)
• While historically, management was limited to gas permeable contact lenses, or penetrating keratoplasty in severe cases, new treatments include early corneal collagen cross-linking (CXL), scleral contact lenses, Bowman's membrane transplants, and topography-guided photokeratoplasty with CXL. (optometrytimes.com)
• They first created the material with a collagen-based bio-ink containing encapsulated corneal keratocytes from donor corneas. (reviewofoptometry.com)
• PRK combined with corneal collagen cross-linking had a transient effect on corneal innervation and a significant effect on keratocyte density for up to 4 years postoperatively, researchers found.The researchers prospectively analyzed 17 eyes of 17 patients with progressive keratoconus who underwent PRK with corneal collagen cross-linking. (linkoph.com)
• These compounds denature proteins and saponify fatty acid, causing collagen breakdown and lysis of keratocytes, the cells responsible for corneal transparency. (emdocs.net)
• Specifically, human corneal keratocytes were seeded at 5 × 105 cells/mL in the hydrogels with a collagen concentration of 3.0 mg/mL. (keele.ac.uk)
• Corneal Collagen Cross-Linking for Keratoconus. (farideh.ca)

Endothelial cells3

• In vitro propagation of primary and extended life span murine corneal endothelial cells. (nih.gov)
• The Descemet's membrane was absent as the flat and polygonal endothelial cells directly adhered to the deepest corneal lamella. (unipa.it)
• Histopathologically, intracellular accumulations of glycosaminoglycans are found in keratocytes and endothelial cells. (biomedcentral.com)

Inflammation3

• Medical management includes managing corneal microtrauma and inflammation. (optometrytimes.com)
• PURPOSE: To compare corneal inflammation after syngeneic and allogeneic penetrating keratoplasty (PK) with miniature Keratoprosthesis (m-KPro) implantation in mice. (harvard.edu)
• Corneal inflammation was assessed by determining the frequencies of CD45(+) leukocytes, CD4(+) T cells, CD11b(+) cells, and Gr-1(+) granulocytes/monocytes by flow cytometry at 2, 4, and 8 weeks post transplantation. (harvard.edu)

Keratoconus5

• Patients with any stage of keratoconus, including those with corneal grafts, require clinical differentiation between allergic conjunctivitis and dry eye. (optometrytimes.com)
• The consequence is increased curvature and corneal thinning - the hallmarks of keratoconus. (ijkecd.com)
• Redistribution of corneal curvature leads to a redistribution of corneal stress, interrupting the biomechanical cycle of keratoconus disease progression, and in some cases, reversing the process. (ijkecd.com)
• Originally designed for the correction of mild myopia in normal eyes, the segments are now commonly used to regularize the corneal surface in keratoconus to improve UCVA and CVA and improve the ability to wear soft contact lenses. (ijkecd.com)
• Patients with a parent, sibling, or child who has keratoconus have 15 to 67 times higher risk in developing corneal ectasia compared to patients with no affected relatives. (ophthalmologybreakingnews.com)

Normal and diseased1

• In addition, retinoic acid is introduced into the serum-free culture medium either in 2D or 3D environment to evaluate its potential in modulating the phenotype of both normal and diseased human corneal keratocytes. (bl.uk)

Penetrating keratoplasty3

• A corneal dystrophy was diagnosed and penetrating keratoplasty was recommended but the patient was hesitant to undergo surgery. (biomedcentral.com)
• This report presents the first case of a histologically evident exacerbation of macular corneal dystrophy after PTK and emphasizes the relevance of thorough pre-interventional diagnosis and patient selection to consider other therapeutic approaches, such as penetrating keratoplasty. (biomedcentral.com)
• Chung SH, Kim HK, Kim MS. Corneal endothelial cell loss after penetrating keratoplasty in relation to preoperative recipient endothelial cell density. (jamanetwork.com)

Treatment of corneal diseases2

• Our study offers a clinical potential for a minimally invasive macromolecular treatment of corneal diseases. (arvojournals.org)
• This graft is indicated for the treatment of corneal diseases, ulcerations, descemetocele, perforations, feline corneal sequestrum, and trauma where pharmaceutical interventions and/or temporary ocular bandage coverage have failed or are of no use. (petseyes.cn)

Fibroblasts and Myofibroblasts1

• ALDH3A1 expression was also reduced in corneal fibroblasts and myofibroblasts as determined by immunolabeling of the cells in culture (n = 3) and in diseased corneal tissues in situ (n = 2). (northumbria.ac.uk)

Properties of corneal1

• It is hypothesized that retinoic acid supplementation in culture medium is able to modulate specific chemical cues especially the matrix metalloproteases (MMPs) to retain quiescent properties of corneal keratocytes previously exposed to serum in vitro. (bl.uk)

Morphology6

• To analyze the morphology of corneal epithelial cells and keratocytes by in vivo confocal microscopy (IVCM) in patients with herpes simplex keratitis (HSK) as associated with corneal innervation. (harvard.edu)
• Changes in morphology and density of the superficial and basal epithelial cells, as well as stromal keratocytes were assessed by two masked observers. (harvard.edu)
• Changes in superficial epithelial cell density and morphology correlated strongly with total nerve length, number, and corneal sensation. (harvard.edu)
• This study aimed to explore the effects of Acacia honey (AH) on corneal keratocytes morphology, proliferative capacity, cell cycle, gene and protein analyses. (blogspot.com)
• Corneal keratocytes cultured in media supplemented with 0.025% AH showed an increase in proliferative capacity while retaining their morphology, gene and protein expressions with normal cell cycle. (blogspot.com)
• Confocal microscopy was used preoperatively and 1, 3, 6, 12, 18 and 48 months postoperatively to assess corneal morphology. (linkoph.com)

Transparency5

• Transparency is essential for normal corneal function. (northumbria.ac.uk)
• Recent studies suggest that corneal cells express high levels of so-called corneal crystallins, such as aldehyde dehydrogenase (ALDH) and transketolase (TKT) that contribute to maintaining cellular transparency. (northumbria.ac.uk)
• Previous studies on keratocytes from species such as bovine and rabbit indicate that the transformation from the normal to repair phenotype is accompanied by a loss of corneal crystallin expression, which may be associated with loss of cellular transparency. (northumbria.ac.uk)
• Extrapolating from studies of bovine and rabbit, the reduced expression of ALDH3A1 may contribute to the loss of corneal transparency experienced by human patients after injury and refractive surgeries. (northumbria.ac.uk)
• This graft preserves the complex features that are essential to corneal function, including light transparency, resistance to ultraviolet light, insensitivity to photons and good tensile strength. (petseyes.cn)

Ophthalmology4

• Academic Ophthalmology discovered mesenchymal stem cells residing in the corneal stoma, therefore redefining the conventional keratocyte. (nottingham.ac.uk)
• Despite her clinical work and experience she gained in small and equine ophthalmology over the years, her passion and focus has been on corneal research in dogs and cats. (rvc.ac.uk)
• Christiane is currently a clinical Senior Lecturer of the Ophthalmology Department, Clinical Sciences and Services, Queen Mother Hospital for Animals and undertakes corneal research work in the stem cell group of Dr. Deborah Guest (CSS, RVC). (rvc.ac.uk)
• PetsEyes BioCorneaVet is a kind of Decellularized Porcine Corneal Xenografts, specifically developed and designed in the use of veterinary ophthalmology. (petseyes.cn)

Refractive4

• Jose I. Barraquer, MD, described corneal lamellar surgery for the correction of refractive error in 1949. (aao.org)
• After the microkeratome cut the corneal cap, it was stabilized with suction in the inverted position on a forming die while the refractive cut was made by a second pass of the instrument on the stromal side of the corneal cap. (aao.org)
• This technique involved less trauma to the corneal cap, but the results remained suboptimal secondary to unpredictable refractive changes and irregular astigmatism. (aao.org)
• The CXL and intracorneal rings have theoretical complementary effects on corneal biomechanics and refractive parameters. (ijkecd.com)

Transplantation3

• This knowledge will aid in constructing more robust ex-vivo corneal epithelial cell constructs for transplantation to treat ocular surface disease. (cardiff.ac.uk)
• The conventional treatment for most existing corneal scars is corneal transplantation (penetrating or lamellar keratoplasty) [ 2 ]. (biomedcentral.com)
• Donor age and corneal endothelial cell loss 5 years after successful corneal transplantation: Specular Microscopy Ancillary Study Results. (jamanetwork.com)

Vitro4

• Canine Corneal Stromal Cells have multipotent mesenchymal stromal cell properties in vitro. (rvc.ac.uk)
• Innoprot also offers optimized medium and reagents for the growth of Human Keratocytes which are quality tested together and guaranteed to give maximum performance as a global solution for in vitro HK culture. (innoprot.com)
• Ophthalmologica Corneal Endothelial Cytotoxicity of Riboflavin/UVA Treatment in vitro Wollensak, G. (kcglobal.org)
• Keratocyte cytotoxicity of riboflavin/UVAtreatment in vitro Wollensak, G.;Spörl, E. (kcglobal.org)

Ocular4

• Amnion has been extensively used for the regeneration of many tissues including the ocular surface where it has become a standard treatment for corneal defects. (nottingham.ac.uk)
• Corneal thickness measurements and visual function abnormalities in ocular hypertensive patients. (sciendo.com)
• Structural, ultrastructural, and morphometric study of the zebrafish ocular surface: a model for human corneal diseases? (unipa.it)
• Conclusions: The zebrafish ocular surface showed significant differences compared to the human, such as the absence of Bowman's layer, Descemet's membrane and corneal nerve fibers, the reduced stromal thickness, and the presence of rodlet cells. (unipa.it)

Innervation1

• We demonstrate that these changes strongly correlate with changes in corneal innervation. (harvard.edu)

Donor2

• Human corneal keratocytes isolated from three donors expressed ALDH1A1 and ALDH3A1 mRNA, and one donor also expressed ALDH2 and TKT. (northumbria.ac.uk)
• Primary human CSSC (hCSSC) from donor corneal rims were cultivated to passage 3 and co-cultured with mouse macrophage RAW264.7 cells induced to M1 pro-inflammatory phenotype by treatment with interferon-γ and lipopolysaccharides, or to M2 anti-inflammatory phenotype by interleukin-4, in a Transwell system. (biomedcentral.com)

Keratitis1

• Background Techniques used to diagnose Acanthamoeba keratitis (AK), a sight-threatening corneal infection, are either insensitive or time-consuming. (eg.net)

Macular2

• Phenotype of macular corneal dystrophy in Labrador Retrievers: a multicenter study. (rvc.ac.uk)
• Macular corneal dystrophy is categorized as a stromal dystrophy. (biomedcentral.com)

Limbal stem3

• Damage to limbal stem cells and the corneal surface can lead to visual impairment and blindness/debilitation. (nottingham.ac.uk)
• Here, different methods of decellularisation are used to identify the best method which maintains the structural integrity of scaffolds as well as allowing recellularisation of limbal stem cells to model corneal epithelial growth. (bl.uk)
• Recently, the lab's focus is on the development of a new induced pluripotent stem cell-driven source of corneal epithelial stem cells for limbal stem cell deficiency. (cedars-sinai.edu)

Murine corneal1

• Chemokine decoy receptor D6 mimicking trap (D6MT) prevents allosensitization and immune rejection in murine corneal allograft model. (harvard.edu)

Blindness3

• Yet there is a significant shortage of corneas available to transplant, with 10 million people worldwide requiring surgery to prevent corneal blindness as a result of diseases such as trachoma, an infectious eye disorder. (neurosciencenews.com)
• In addition, almost 5 million people suffer total blindness due to corneal scarring caused by burns, lacerations, abrasion or disease. (neurosciencenews.com)
• Corneal blindness: A global perspective. (reviewofoptometry.com)

Defects1

• But the professional veterinary ophthalmologists are the final decision-maker in using this graft or other methods to repair the corneal defects. (petseyes.cn)

Quiescent2

• Similar effect is also postulated onto fibroblast taken from keratoconic corneas, in which modulation of the MMPs enables the keratoconic fibroblast to de-differentiate into a more normal, quiescent keratocyte. (bl.uk)
• The morphological changes, gene and protein expressions of aldehyde dehydrogenase (ALDH), marker for quiescent keratocytes and vimentin, marker for fibroblasts were detected using q-RTPCR and immunocytochemistry respectively. (blogspot.com)

Wound3

• Continuous epidermal growth factor delivery in corneal epithelial wound healing. (mcmaster.ca)
• Acacia honey is a natural product which has proven to have therapeutic effects on skin wound healing , but its potential healing effects in corneal wound healing have not been studied. (blogspot.com)
• Concise review: stem cells for corneal wound healing. (cedars-sinai.edu)

Stem1

• In 2022 Christiane started the RVC's lead on the corneal stem cell research ("CornealCellVision") and clinical application/translational aspect of stem cells. (rvc.ac.uk)

Phenotype2

• Stromal injury leads to the appearance of repair phenotype keratocytes, the corneal fibroblast and myofibroblast. (northumbria.ac.uk)
• We conclude that expression of the crystallin ALDH3A1 is decreased in repair phenotype human keratocytes, compared to normal human keratocytes. (northumbria.ac.uk)

Trauma1

• In the event of corneal injury or trauma, however, the keratocytes differentiate into active, synthesizing cells and rapidly replace damaged stromal matrix. (innoprot.com)

Cells13

• We are currently developing methodologies to develop these cells as a bankable technology for corneal regeneration and engineering proposes. (nottingham.ac.uk)
• The density of basal epithelial cells, anterior keratocytes, and posterior keratocytes did not show statistical significance between patients and controls. (harvard.edu)
• This thesis describes the role of mechanical and chemical cues to preserve corneal cells' function. (bl.uk)
• Each vial of Human Keratocytes contains more than 500.000 viable cells. (innoprot.com)
• Human corneal epithelial cells were collected by scraping and keratocytes were isolated by collagenase digestion from cadaveric corneas. (northumbria.ac.uk)
• The cells were either processed immediately (freshly isolated keratocytes) or were cultured in the presence of 10% fetal bovine serum or transforming growth factor-β to induce transformation to the corneal fibroblast and myofibroblast phenotypes, respectively. (northumbria.ac.uk)
• Corneal epithelial cells expressed ALDH1A1, ALDH2, ALDH3A1 and TKT. (northumbria.ac.uk)
• ALDH3A1 enzyme activity was not detectable in corneal fibroblasts (n = 6) but was readily detected in corneal epithelial cells (0.29 ± 0.1 U/mg protein, n = 4) and keratocytes (0.05 ± 0.009 U/mg protein, n = 7). (northumbria.ac.uk)
• Exosomes from normal and diabetic human corneolimbal keratocytes differentially regulate migration, proliferation and marker expression of limbal epithelial cells. (cedars-sinai.edu)
• This study examined the role of chemokine receptor D6 expression in a corneal allograft rejection, investigated the modulation of D6 expression in cells, and determined the effect of D6 on graft survival. (harvard.edu)
• Multipotent periocular cells, while it began with the neural crest, go through distinctive modifications in gene appearance because they migrate to create corneal endothelium and keratocytes (Bi and Lwigale, 2019). (phytid.org)
• We also describe three-dimensional characterisation of a populace of extracellular matrix cords that link the epithelial basement membrane with subjacent neural crest cells and present a model speculating how they might serve a mechanical role in the development of Kgp-IN-1 corneal curvature. (phytid.org)
• The porous scaffold structure is an ideal platform for the ingrowth of nerve and vessels as well as the repopulation of host cells like keratocytes and epithelial cells. (petseyes.cn)

Anterior1

• Primary study outcome measures were mean superficial epithelial cell density, mean basal epithelial cell density and mean anterior and mid/posterior keratocyte density. (linkoph.com)

Dystrophy3

• Reis-Bücklers, formerly known as Granular corneal dystrophy type III or Corneal Dystrophy of Bowman's type I, typically present with normal corneas at birth but develop painful recurrent erosions, opacification, and progressive vision loss within the first decade of life (1). (eyerounds.org)
• The hyaline-like material consists of rod-like bodies ultrastructurally, which helps distinguish it from Thiel-Behnke corneal dystrophy (1, 2). (eyerounds.org)
• Lattice corneal dystrophy (LCD) is the most common of the corneal epithelial-stromal dystrophies. (eyerounds.org)

Inflammatory4

• NV is believed to result from an inflammatory or hypoxic disruption of an exquisitely balanced corneal immune system. (medscape.com)
• The resulting imbalance may initiate and perpetuate corneal inflammatory pathways to result in the structural abnormalities seen with disease progression. (optometrytimes.com)
• Corneal clarity and the expression of mouse inflammatory and fibrosis genes were examined. (biomedcentral.com)
• Additionally, understanding keratocan in the corneal inflammatory response is a topic of interest. (mdbioproducts.com)

Epithelial-stromal1

• The 2015 International Committee for Classification of Corneal Dystrophies (IC3D) classification system has divided corneal dystrophies into 4 categories: epithelial and subepithelial dystrophies, epithelial-stromal dystrophies, stromal dystrophies, and endothelial dystrophies. (eyerounds.org)

Corneas3

• Incidence of subsequent corneal graft rejection is estimated by one study to be 1.7 times higher in a setting of vascularized rather than nonvascularized host corneas. (medscape.com)
• Recycling corneas overcomes the major engineering and costly challenge of recreating the corneal architecture artificially. (nottingham.ac.uk)
• Technology has once again come to the rescue-this time to in hopes of providing artificial corneas for the nearly five million people worldwide who have lost vision due to infection-related corneal scarring. (reviewofoptometry.com)

Human3

• Differential effects of HSV-1 and HCMV infection on adhesion molecule expression on human corneal keratocytes. (nih.gov)
• Here we investigated if a similar loss occurs with human keratocyte repair phenotypes. (northumbria.ac.uk)
• 2. Doughty MJ, Zaman ML. Human corneal thickness and its impact on intraocular pressure measures: a review and meta-analysis approach. (sciendo.com)

Curvature2

• On the contrary, when you add material to the center or remove it from the corneal periphery, you obtain a steeped surface curvature. (ijkecd.com)
• Although the ICRS mechanism is not totally understood, segment insertion induces a change in corneal volume peripherally and overall corneal curvature, with possible modification of corneal biomechanical properties. (ijkecd.com)

Neovascularization1

• Short-term regression of corneal neovascularization with combination therapy of argon green laser photocoagulation and subconjunctival bevacizumab. (doximity.com)

Keratan1

• Differential expression of the keratan sulphate proteoglycan, keratocan, during chick corneal embryogenesis. (mdbioproducts.com)

Graft rejection1

• The effect of ABO blood incompatibility on corneal transplant failure in conditions with low-risk of graft rejection. (jamanetwork.com)

Clinical2

• Corneal vascularization due to corneal contact lenses: the clinical picture. (medscape.com)
• CRS Today Can we measure Corneal Biomechanical Constants in a Clinical Setting? (kcglobal.org)

Recurrent1

• It is typically an autosomal dominant, bilateral disease that typically presents toward the end of the first decade of life with symptoms of recurrent corneal erosions and decreased vision. (eyerounds.org)

Retinal1

• There is little retinal degeneration unlike that often seen in other mucopolysaccharidoses but the corneal clouding often precludes detailed examination. (arizona.edu)

Patients1

• Patients and methods Corneal swabs and scrapings from 95 cases suspected to have AK were examined by microscopy, culture on non-nutrient agar seeded with the avian fecal Escherichia coli AFEC49, and by PCR targeting the 18S rRNA gene. (eg.net)

Nerve1

• The immunohistochemical stain of neurofilaments failed to demonstrate corneal nerve fibers. (unipa.it)

Pachymetry4

• Central corneal thickness measurement with the Pentacam Scheimpflug system, optical low-coherence reflectometry pachymeter, and ultrasound pachymetry. (sciendo.com)
• 5. Al-Mezaine HS, Al-Amro SA, Kangave D, Sadaawy A, Wehaib TA, Al-Obeidan S. Comparison between central corneal thickness measurements by oculus pentacam and ultrasonic pachymetry. (sciendo.com)
• 13. Miglior S, Albe E, Guareschi M, Mandelli G, Gomarasca S, Orzalesi N. Intraobserver and interobserver reproducibility in the evaluation of ultrasonic pachymetry measurements of central corneal thickness. (sciendo.com)
• 1 Modern diagnostic techniques include Scheimpflug imaging, corneal wavefront, pachymetry analysis, and epithelial mapping. (optometrytimes.com)