Compounds containing carbohydrate or glycosyl groups linked to phosphatidylinositols. They anchor GPI-LINKED PROTEINS or polysaccharides to cell membranes.
The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.
The systematic study of the structure and function of the complete set of glycans (the glycome) produced in a single organism and identification of all the genes that encode glycoproteins.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
The characteristic 3-dimensional shape of a carbohydrate.
Proteins found mainly in icosahedral DNA and RNA viruses. They consist of proteins directly associated with the nucleic acid inside the NUCLEOCAPSID.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.
Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
An N-acyl derivative of neuraminic acid. N-acetylneuraminic acid occurs in many polysaccharides, glycoproteins, and glycolipids in animals and bacteria. (From Dorland, 28th ed, p1518)
A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.
Enzymes that catalyze the transfer of glycosyl groups to an acceptor. Most often another carbohydrate molecule acts as an acceptor, but inorganic phosphate can also act as an acceptor, such as in the case of PHOSPHORYLASES. Some of the enzymes in this group also catalyze hydrolysis, which can be regarded as transfer of a glycosyl group from the donor to water. Subclasses include the HEXOSYLTRANSFERASES; PENTOSYLTRANSFERASES; SIALYLTRANSFERASES; and those transferring other glycosyl groups. EC 2.4.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A mass spectrometric technique that is used for the analysis of large biomolecules. Analyte molecules are embedded in an excess matrix of small organic molecules that show a high resonant absorption at the laser wavelength used. The matrix absorbs the laser energy, thus inducing a soft disintegration of the sample-matrix mixture into free (gas phase) matrix and analyte molecules and molecular ions. In general, only molecular ions of the analyte molecules are produced, and almost no fragmentation occurs. This makes the method well suited for molecular weight determinations and mixture analysis.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
Enzymes that catalyze the transfer of N-acetylglucosamine from a nucleoside diphosphate N-acetylglucosamine to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
The hepatitis B antigen within the core of the Dane particle, the infectious hepatitis virion.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.
A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Carbohydrates covalently linked to a nonsugar moiety (lipids or proteins). The major glycoconjugates are glycoproteins, glycopeptides, peptidoglycans, glycolipids, and lipopolysaccharides. (From Biochemical Nomenclature and Related Documents, 2d ed; From Principles of Biochemistry, 2d ed)
SUGARS containing an amino group. GLYCOSYLATION of other compounds with these amino sugars results in AMINOGLYCOSIDES.
Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.
Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)
The simultaneous analysis, on a microchip, of multiple samples or targets arranged in an array format.
Any compound containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety such as an acylglycerol (see GLYCERIDES), a sphingoid, a ceramide (CERAMIDES) (N-acylsphingoid) or a prenyl phosphate. (From IUPAC's webpage)
Oligosaccharides containing three monosaccharide units linked by glycosidic bonds.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A trisaccharide antigen expressed on glycolipids and many cell-surface glycoproteins. In the blood the antigen is found on the surface of NEUTROPHILS; EOSINOPHILS; and MONOCYTES. In addition, CD15 antigen is a stage-specific embryonic antigen.
A class of animal lectins that bind specifically to beta-galactoside in a calcium-independent manner. Members of this class are distiguished from other lectins by the presence of a conserved carbohydrate recognition domain. The majority of proteins in this class bind to sugar molecules in a sulfhydryl-dependent manner and are often referred to as S-type lectins, however this property is not required for membership in this class.
Any of various enzymatically catalyzed post-translational modifications of PEPTIDES or PROTEINS in the cell of origin. These modifications include carboxylation; HYDROXYLATION; ACETYLATION; PHOSPHORYLATION; METHYLATION; GLYCOSYLATION; ubiquitination; oxidation; proteolysis; and crosslinking and result in changes in molecular weight and electrophoretic motility.
Enzymes that catalyze the transfer of galactose from a nucleoside diphosphate galactose to an acceptor molecule which is frequently another carbohydrate. EC 2.4.1.-.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A hexosyltransferase involved in the transfer of disaccharide molecules to the peptidoglycan structure of the CELL WALL SKELETON. It plays an important role in the genesis of the bacterial CELL WALL.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)
Ligand-binding assays that measure protein-protein, protein-small molecule, or protein-nucleic acid interactions using a very large set of capturing molecules, i.e., those attached separately on a solid support, to measure the presence or interaction of target molecules in the sample.
A group of enzymes with the general formula CMP-N-acetylneuraminate:acceptor N-acetylneuraminyl transferase. They catalyze the transfer of N-acetylneuraminic acid from CMP-N-acetylneuraminic acid to an acceptor, which is usually the terminal sugar residue of an oligosaccharide, a glycoprotein, or a glycolipid. EC 2.4.99.-.
Established cell cultures that have the potential to propagate indefinitely.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Enzymes catalyzing the transfer of fucose from a nucleoside diphosphate fucose to an acceptor molecule which is frequently another carbohydrate, a glycoprotein, or a glycolipid molecule. Elevated activity of some fucosyltransferases in human serum may serve as an indicator of malignancy. The class includes EC 2.4.1.65; EC 2.4.1.68; EC 2.4.1.69; EC 2.4.1.89.
Enzymes that catalyze the transfer of mannose from a nucleoside diphosphate mannose to an acceptor molecule which is frequently another carbohydrate. The group includes EC 2.4.1.32, EC 2.4.1.48, EC 2.4.1.54, and EC 2.4.1.57.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Databases devoted to knowledge about specific chemicals.
Carbohydrate antigens expressed by malignant tissue. They are useful as tumor markers and are measured in the serum by means of a radioimmunoassay employing monoclonal antibodies.
A family of SIALIC ACID binding proteins found in vertebrate species. They are transmembrane proteins which act as cell surface receptors for a variety of sialylated GLYCOCONJUGATES. While a subset of siglec protein subtypes are evolutionarily conserved between mammalian species, there are many others that are species specific.
Proteins prepared by recombinant DNA technology.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
High molecular weight mucoproteins that protect the surface of EPITHELIAL CELLS by providing a barrier to particulate matter and microorganisms. Membrane-anchored mucins may have additional roles concerned with protein interactions at the cell surface.
Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.
The N-acetyl derivative of galactosamine.
Proteins found in any species of bacterium.
A mass spectrometry technique using two (MS/MS) or more mass analyzers. With two in tandem, the precursor ions are mass-selected by a first mass analyzer, and focused into a collision region where they are then fragmented into product ions which are then characterized by a second mass analyzer. A variety of techniques are used to separate the compounds, ionize them, and introduce them to the first mass analyzer. For example, for in GC-MS/MS, GAS CHROMATOGRAPHY-MASS SPECTROMETRY is involved in separating relatively small compounds by GAS CHROMATOGRAPHY prior to injecting them into an ionization chamber for the mass selection.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Sites on an antigen that interact with specific antibodies.
A mass spectrometry technique used for analysis of nonvolatile compounds such as proteins and macromolecules. The technique involves preparing electrically charged droplets from analyte molecules dissolved in solvent. The electrically charged droplets enter a vacuum chamber where the solvent is evaporated. Evaporation of solvent reduces the droplet size, thereby increasing the coulombic repulsion within the droplet. As the charged droplets get smaller, the excess charge within them causes them to disintegrate and release analyte molecules. The volatilized analyte molecules are then analyzed by mass spectrometry.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A plant genus in the family LILIACEAE (sometimes classified as Amaryllidaceae). Galanthus nivalis L. is the source of GALANTHAMINE.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
An indolizidine alkaloid from the plant Swainsona canescens that is a potent alpha-mannosidase inhibitor. Swainsonine also exhibits antimetastatic, antiproliferative, and immunomodulatory activity.
CELL LINE derived from the ovary of the Chinese hamster, Cricetulus griseus (CRICETULUS). The species is a favorite for cytogenetic studies because of its small chromosome number. The cell line has provided model systems for the study of genetic alterations in cultured mammalian cells.
A subfamily in the family MURIDAE, comprising the hamsters. Four of the more common genera are Cricetus, CRICETULUS; MESOCRICETUS; and PHODOPUS.
Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with an alcohol to form an acetal. They include both alpha- and beta-mannosides.
Chromatographic techniques in which the mobile phase is a liquid.
Use of a metal casting, usually with a post in the pulp or root canal, designed to support and retain an artificial crown.
Compounds consisting of glucosamine and lactate joined by an ether linkage. They occur naturally as N-acetyl derivatives in peptidoglycan, the characteristic polysaccharide composing bacterial cell walls. (From Dorland, 28th ed)
An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.
A group of recessively inherited diseases characterized by the intralysosomal accumulation of G(M2) GANGLIOSIDE in the neuronal cells. Subtypes include mutations of enzymes in the BETA-N-ACETYLHEXOSAMINIDASES system or G(M2) ACTIVATOR PROTEIN leading to disruption of normal degradation of GANGLIOSIDES, a subclass of ACIDIC GLYCOSPHINGOLIPIDS.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
Glycoproteins found on the membrane or surface of cells.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Its core domain binds TGF-beta family ligands and its heparan sulfate chains bind bFGF. It acts as a reservoir of ligand for ... TGFβR3 (β-glycan) "Prosite Documentation PDOC00223". Archived from the original on 2011-05-25. Retrieved 2006-07-01. Fliesler ...
These glycans have a Man5GlcNAc2 core with processing on the 3-arm that resembles so-called complex-type glycans. The ... Disruption of Golgi alpha-mannosidase II with swainsonine induces hybrid-type glycans. ...
This results in a deaminated protein or peptide and a free glycan. PNGase F has a molecular weight of 35,500 and consists of a ... It cleaves all asparagine-linked complex, hybrid, or high mannose oligosaccharides unless the core GlcNAc contains an alpha 1,3 ... N-linked glycans can provide structural components of cell walls and extracellular matrices, modify protein stability and ... The asparagine residue, from which the glycan is removed, is deaminated to aspartic acid. PNGase F requires a minimum of two ...
Ellies, L.G.; Tsuboi, S.; Petryniak, B.; Lowe, J.B.; Fukuda, M.; Marth, J.D. (1998). "Core 2 O-glycan biosynthesis ... Green, R.S.; Stone, E.L. Tenno; Lehtonen, E.; Farquhar, M.G.; Marth, J.D. (2007). "Mammalian N-glycan branching protects ... These studies further explained how glycan linkages contribute to the origins of disease at the metabolic and cellular levels. ... Marth's early studies of glycosylation and glycan linkages revealed a profound effect on immunity and contributed to the ...
... a strategy for the analysis of N-glycans by carbon LC-ESI-MS and its application to fibrin N-glycans". Anal. Chem. 79 (13): ... Variety is found in the degree of core fucosylation and in the type of sialic acid and galactose linkage. The image at the left ... Fibrin from different animal sources is generally glycosylated with complex type biantennary asparagine-linked glycans. ...
Park C, Meng L, Stanton LH, Collins RE, Mast SW, Yi X, Strachan H, Moremen KW (Oct 2005). "Characterization of a human core- ... 1996). "Influence of N-linked glycans in V4-V5 region of human immunodeficiency virus type 1 glycoprotein gp160 on induction of ... 1993). "Functional role of the glycan cluster of the human immunodeficiency virus type 1 transmembrane glycoprotein (gp41) ... Epididymis-specific alpha-mannosidase precursor Mannosidase alpha class 2B member core-specific lysosomal alpha-1,6-Mannosidase ...
The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition ... small amounts of these N-glycans also bear bisecting GlcNAc and α-2,6 linked sialic acid residues. The other part of an ...
2003). "Sialic acid capping of CD8beta core 1-O-glycans controls thymocyte-major histocompatibility complex class I interaction ...
Phosphatidylinositol-glycan biosynthesis class F protein is a protein that in humans is encoded by the PIGF gene. This gene ... The GPI-anchor is a glycolipid which contains three mannose molecules in its core backbone. The GPI-anchor is found on many ... "Entrez Gene: PIGF phosphatidylinositol glycan anchor biosynthesis, class F". Gerdin AK (2010). "The Sanger Mouse Genetics ...
In the fucose-containing glycan structures, fucosylated glycans, fucose can exist as a terminal modification or serve as an ... The α(1→3) linked core of fucose is a suspected carbohydrate antigen for IgE-mediated allergy. Two structural features ... It is found on N-linked glycans on the mammalian, insect and plant cell surface. Fucose is the fundamental sub-unit of the ... In human N-linked glycans, fucose is most commonly linked α-1,6 to the reducing terminal β-N-acetylglucosamine. However, fucose ...
... which contain N-glycans with structural similarities to plant N-glycans. Ever since then, core α1,3-fucose emerged as the ... Much later, both xylose and core α1,3-fucose were revealed as heart pieces of two independent glycan epitopes for rabbit IgG. ... 1993). "Fucose alpha 1,3-linked to the core region of glycoprotein N-glycans creates an important epitope for IgE from honeybee ... Examples of cross-reactive plant and insect N-glycans. Their common relevant feature is the core alfa1,3-linked fucose. ...
... core protein xylosyltransferase, UDP-D-xylose:proteoglycan core protein beta-D-xylosyltransferase, UDP-xylose-core protein beta ... This enzyme participates in the biosynthesis of chondroitin sulfate and glycan structures. XYLT1 XYLT2 Xylosyltransferase ... Other names in common use include UDP-D-xylose:core protein beta-D-xylosyltransferase, UDP-D-xylose: ... proteoglycan core protein beta-d-xylosyltransferase and its first isoform XT-II". J. Mol. Biol. 304 (4): 517-28. doi:10.1006/ ...
The core glycan structure of GPI anchors is Man-α-1,2-Man-α-1,6-Man-α-1,4-GlcN-inositol (Man: mannose, GlcN: glucosaminyl), ... "A Synthetic Glycan Microarray Enables Epitope Mapping of Plant Cell Wall Glycan-Directed Antibodies". Plant Physiology. 175 (3 ... The complex glycan structures are then elaborated by a suite of glycosyltransferases, the majority of which are bio-chemically ... The structure of the AG glycans consists of a backbone of β-1,3 linked galactose (Gal), with sidechains of β-1,6 linked Gal and ...
"Quality control of glycoproteins bearing truncated glycans in an ALG9-defective (CDG-IL) patient". Glycobiology. 19 (8): 910-7 ... The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core- ...
Core 2 glycans terminate in galactose or sialic acid, whereas core 1 is branched and has potential for large carbohydrate ... which on cancer cells changes expression from long core 2 type O-glycosylation to shorter core 1 type O-glycosylation. ... In its normal function, galectin-1 binds to glycans on the CD4 co-receptor of T cells to prevent auto reactivity. When HIV is ... They can also hinder adhesion by binding to two glycans on the same cell, which blocks the integrin binding site. Galectin-8 is ...
The N-glycans attached to this site are predominantly core-fucosylated biantennary structures of the complex type. In addition ... small amounts of these N-glycans also bear bisecting GlcNAc and α-2,6-linked sialic acid residues. The N-glycan composition in ...
It is essential to the formation of Gal beta 1-3(GlcNAc beta 1-6)GalNAc structures and the core 2 O-glycan branch. The gene ... 6-N-acetylglucosaminyltransferase that forms core 2, core 4, and I branches". J Biol Chem. 274 (5): 3215-21. doi:10.1074/jbc. ... 2006). "N-glycans of core2 beta(1,6)-N-acetylglucosaminyltransferase-I (C2GnT-I) but not those of alpha(1,3)-fucosyltransferase ... 2005). "Not core 2 beta 1,6-N-acetylglucosaminyltransferase-2 or -3 but -1 regulates sialyl-Lewis x expression in human ...
Two of the most common structures formed are Core 1 and Core 2. Core 1 is formed by the addition of a galactose sugar onto the ... Absence of O-glycans on mucin proteins changes their 3D shape dramatically and often prevents correct function. Addition of N- ... Core 2 consists of a Core 1 structure with an additional N-acetylglucosamine (GlcNAc) sugar. A poly-N-acetyllactosamine ... Once the core O-fucose has been added, it is often elongated by addition of GlcNAc, galactose and sialic acid. Notch is an ...
"Identification and molecular cloning of a novel glycoside hydrolase family of core 1 type O-glycan-specific endo-alpha-N- ...
Fabini G, Freilinger A, Altmann F, Wilson IB (2001). "Identification of core alpha 1,3-fucosylated glycans and cloning of the ... van Tetering A, Schiphorst WE, van den Eijnden DH, van Die I (1999). "Characterization of a core alpha1-->3-fucosyltransferase ... This enzyme participates in glycan structures - biosynthesis 1. Muller S, Hauser MT (2001). "Cloning and expression of cDNAs ... from the snail Lymnaea stagnalis that is involved in the synthesis of complex-type N-glycans". FEBS Lett. 461 (3): 311-4. doi: ...
"Selected reaction monitoring to differentiate and relatively quantitate isomers of sulfated and unsulfated core 1 O-glycans ... Metabolic labeling of glycans can be used as a way to detect glycan structures. A well known strategy involves the use of azide ... a strategy for the analysis of N-glycans by carbon LC-ESI-MS and its application to fibrin N-glycans". Anal. Chem. 79 (13): ... "Glycans in the immune system and The Altered Glycan Theory of Autoimmunity". J Autoimmun. 57 (6): 1-13. doi:10.1016/j.jaut. ...
Core D also synthesizes glycans for and prints the CFG glycan array (see Core H below) Gene Microarray Core (E), located at The ... of glycans Glycans in immune cell communication Glycans in development and physiology Glycans in cancer biology Glycans in ... From 2001-2009, Core F generated 26 total and conditional knockout mouse lines deficient in glycan-binding proteins or ... Glycan Array Synthesis Core (D), located at The Scripps Research Institute, produces and collects carbohydrate compounds ( ...
The common core 1 O-glycan structure Gal-beta-1-3GalNAc-R is a precursor for many extended mucin-type O-glycan structures in ... Core 1 is synthesized by the transfer of Gal from UDP-Gal to GalNAc-alpha-1-R by core 1 beta-3-galactosyltransferase (C1GALT1 ... Core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase, 1, also known as C1GALT1, is an enzyme which ... Ju T, Brewer K, D'Souza A, Cummings RD, Canfield WM (January 2002). "Cloning and expression of human core 1 beta1,3- ...
This enzyme is highly specific for core 1 structures, with asialofetuin, Gal-beta-1,3-GalNAc and Gal-beta-1,3 (GlcNAc-beta-1,6) ... 3GalNAc residue in O-glycans. J Biol Chem. 2001;276:25697-25704. Maruyama K, Sugano S (1994). "Oligo-capping: a simple method ... Mutation of this gene results in alterations of the typical sulfation pattern of glycan chains, which will alter the ... and Gal3ST-4 for the mucin core-2 trisaccharide". J. Biol. Chem. 279 (11): 10032-41. doi:10.1074/jbc.M311989200. PMID 14701868 ...
Since its introduction, the concept of the bioorthogonal reaction has enabled the study of biomolecules such as glycans, ... proteins, and lipids Earth's inner core Discovered in 1936 by Danish seismologist Inge Lehmann. Square-bottom paper bag In 1868 ...
Currently, there are additional databases: KEGG GLYCAN for glycans and two auxiliary reaction databases called RPAIR (reactant ... is the core of the KEGG resource. It is a collection of pathway maps integrating many entities including genes, proteins, RNAs ... GLYCAN - chemical compounds and glycans REACTION, RPAIR, RCLASS - chemical reactions ENZYME - enzyme nomenclature Health ... chemical compounds, glycans, and chemical reactions, as well as disease genes and drug targets, which are stored as individual ...
... oligosaccharides and core-2-branched O-glycans, but not on type 1 or core-1-branched structures. This gene, which has also been ... and O-glycans". J Biol Chem. 276 (26): 24388-95. doi:10.1074/jbc.M103135200. PMID 11323440. El-Fasakhany FM, Uchimura K, ... and Gal3ST-4 for the mucin core-2 trisaccharide". J. Biol. Chem. 279 (11): 10032-41. doi:10.1074/jbc.M311989200. PMID 14701868 ... 3galNAc residue in O-glycans". J. Biol. Chem. 276 (28): 25697-704. doi:10.1074/jbc.M101558200. PMID 11333265. Chandrasekaran EV ...
... and on core 1 glycans. This enzyme has been implicated in tumor metastasis processes. This gene is different from the GAL3ST3 ... and O-glycans". J. Biol. Chem. 276 (26): 24388-95. doi:10.1074/jbc.M103135200. PMID 11323440. Seko A, Hara-Kuge S, Yamashita K ... 3galNAc residue in O-glycans". J. Biol. Chem. 276 (28): 25697-704. doi:10.1074/jbc.M101558200. PMID 11333265. Ikeda N, Eguchi H ... and Gal3ST-4 for the mucin core-2 trisaccharide". J. Biol. Chem. 279 (11): 10032-41. doi:10.1074/jbc.M311989200. PMID 14701868 ...
It comprises three parts: O antigen (or O polysaccharide) Core oligosaccharide Lipid A A repetitive glycan polymer contained ... outer core and inner core joined by a covalent bond; they are found in the outer membrane of Gram-negative bacteria. The term ... The outer core oligosaccharide chain varies depending on the bacterial strain. The term lipooligosaccharide is used to refer to ... The core domain always contains an oligosaccharide component that attaches directly to lipid A and commonly contains sugars ...
Monné M, Han L, Schwend T, Burendahl S, Jovine L (2008). "Crystal structure of the ZP-N domain of ZP3 reveals the core fold of ... "Murine and human zona pellucida 3 derived from mouse eggs express identical O-glycans". Proc. Natl. Acad. Sci. U.S.A. 100 (26 ... Monné M, Han L, Schwend T, Burendahl S, Jovine L (2008). "Crystal structure of the ZP-N domain of ZP3 reveals the core fold of ...
"Carbohydrate binding specificity of a fucose-specific lectin from Aspergillus oryzae: a novel probe for core Fuchse" Matsumura ... recognition of high mannose-type N-glycans produced by plants and yeast."Glycoconj J. 2005 Nov;22(7-9):453-61. ... 3-Linked to Penultimate Galactose Residues of Trisialyl N-Glycans. Comparison with other sialic acid-specific lectins.J Biol ...
The antenna system is at the core of the chlorophyll molecule of the photosystem II reaction center. That freed electron is ... sugars & glycans Simple. sugars Inositol-P Amino sugars. & sialic acids Nucleotide sugars ...
Within the envelope is an icosahedral core that is 33 to 40 nm in diameter.[10] Inside the core is the RNA material of the ... E1 has 4-5 N-linked glycans and E2 has 11 N-glycosylation sites. ... The core domain of the HCV IRES contains a four-way helical ... The core protein has 191 amino acids and can be divided into three domains on the basis of hydrophobicity: domain 1 (residues 1 ... Alternatively, a frameshift may occur in the Core region to produce an alternate reading frame protein (ARFP).[32] HCV encodes ...
In mammalian cells for example, there exists a mechanism called glycan processing. In this mechanism, the lectin-type ... Hereafter, the polypeptide chain is fed into the central chamber of the 20S core region that contains the proteolytically ... "N-glycan processing in ER quality control". J. Cell Sci. 119 (Pt 21): 4373-80. doi:10.1242/jcs.03225. PMID 17074831 ...
The steroids, all derived from the same fused four-ring core structure, have different biological roles as hormones and ... Helenius A, Aebi M (March 2001). "Intracellular functions of N-linked glycans". Science. 291 (5512): 2364-9. doi:10.1126/ ... are characterized by cleavage of the B ring of the core structure.[45] Other examples of sterols are the bile acids and their ... which contain an isoprenoid tail attached to a quinonoid core of non-isoprenoid origin.[51] Vitamin E and vitamin K, as well as ...
The crystal structure of core gp120 shows an organization with an outer domain, an inner domain with respect to its termini and ... Consequently, insertions in env, which confer more PNGSs on gp120 may be more tolerated by the virus as higher glycan density ... A lower number of bulky glycans improves viral replication efficiency and higher number on the exposed loops aids host immune ... Therefore, a stabilizing selection balance between low and high glycan densities is likely established. ...
van Heijenoort J (March 2001). "Formation of the glycan chains in the synthesis of bacterial peptidoglycan". Glycobiology. 11 ( ... Each endospore contains a core of DNA and ribosomes surrounded by a cortex layer and protected by a multilayer rigid coat ... "The glycan-rich outer layer of the cell wall of Mycobacterium tuberculosis acts as an antiphagocytic capsule limiting the ...
A core protein of glycogenin is surrounded by branches of glucose units. The entire globular granule may contain approximately ... Glycan. *Oligosaccharide nomenclature. *Polysaccharide encapsulated bacteria. References[edit]. *^ Varki A, Cummings R, Esko J ...
Over half of the mass of the trimeric envelope spike is N-linked glycans. The density is high as the glycans shield the ... Contradicting the report from Gallo's group, Montagnier and his colleagues showed that core proteins of this virus were ... The majority of the glycans are therefore stalled as immature 'high-mannose' glycans not normally present on human ... but also display the same degree of immature glycans as presented on the native virus.[34] Recombinant trimeric viral spikes ...
See LAMP1 for more information on both LAMP1 and LAMP2.) Both of these consist of polypeptides of about 40 kD, with the core ... Carlsson SR, Lycksell PO, Fukuda M (July 1993). "Assignment of O-glycan attachment sites to the hinge-like regions of human ...
Aberrant glycan profiles have been described in prostate cancer, and studies have found specific links between the galectin ... Points are assigned based on PSA, grade, stage, age, and percentage of cores positive; the sum yields a 0-10 score, with every ... Most combine stage, grade, and PSA level, and some include the number or percentage of biopsy cores positive, age, and/or other ... Munkley J, Mills IG, Elliott DJ (June 2016). "The role of glycans in the development and progression of prostate cancer". ...
MALDI MS-imaging interest group Imaging MS interest group DFG (German Research Foundation) National Core Facility for MALDI MS- ... "MALDI Imaging Mass Spectrometry Profiling of N-Glycans in Formalin-Fixed Paraffin Embedded Clinical Tissue Blocks and Tissue ...
"KEGG GLYCAN: G00112". www.genome.jp. Retrieved 2019-08-13. "KEGG GLYCAN: G00122". www.genome.jp. Retrieved 2019-08-13. Umeda M ... The SeV virion also contains the nucleocapsid core, which is composed of the genomic RNA, the nucleocapsid protein (NP), the ... "KEGG GLYCAN: G00116". www.genome.jp. Retrieved 2019-08-13. "KEGG GLYCAN: G00117". www.genome.jp. Retrieved 2019-08-13. Vukelić ... a resource of glycan-mediated host-pathogen interactions. Others are available through KEGG Glycan Database, PubChem compound ...
Vonderach M, Byrne DP, Barran PE, Eyers PA, Eyers CE (2019). "DNA Binding and Phosphorylation Regulate the Core Structure of ... "Discrimination of epimeric glycans and glycopeptides using IM-MS and its potential for carbohydrate sequencing". Nature ... and gas-phase characterisation of modified peptides and glycans. During her time in Manchester, she also contributed to ... protein kinase complexes and glycan conformation, and several highly-accessed reviews[citation needed] on the subject of ion ...
"Printed covalent glycan array for ligand profiling of diverse glycan binding proteins". Proceedings of the National Academy of ... Hanson, S. R.; Culyba, E. K.; Hsu, T.-L.; Wong, C.-H.; Kelly, J. W.; Powers, E. T. (2009-03-03). "The core trisaccharide of an ... The original synthetic methods developed by Wong along with his work on the development of glycan microarrays for the high- ... Krasnova, Larissa; Wong, Chi-Huey (2016-06-02). "Understanding the Chemistry and Biology of Glycosylation with Glycan Synthesis ...
The great majority of PPI interfaces reflects the composition of protein surfaces, rather than the protein cores, in spite of ... Glycan-protein interactions 3did Allostery Biological network Biological machines Enzyme catalysis Human interactome ... domain SAM domains are composed by five helices forming a compact package with a conserved hydrophobic core. These domains, ...
The so-called "9 + 2" structure is characteristic of the core of the eukaryotic flagellum called an axoneme. At the base of a ... The archaellins are typically modified by the addition of N-linked glycans which are necessary for proper assembly or function ... During flagellar assembly, components of the flagellum pass through the hollow cores of the basal body and the nascent filament ... including the observation that almost all of the core flagellar proteins have known homologies with non-flagellar proteins. ...
Accordingly, a chemoenzymatic strategy was developed for the efficient synthesis of core-fucos ... Substrate specificity studies of human FUT8 using 77 structurally-defined N-glycans as acceptors showed a strict requirement ... Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans A. D. Calderon, Y. Liu, X. ... Substrate specificity of FUT8 and chemoenzymatic synthesis of core-fucosylated asymmetric N-glycans† ...
... C2GnT1 is essential for the synthesis of core 2-branched O-linked carbohydrates terminated with sLex (C2-O-sLex). Our goal was ... We also examined mRNA levels of the enzyme core 2 beta1,6-N-acetylglucosaminyltransferase (C2GnT1) in 20 well, 15 moderately, ... The activity of the enzyme core 2 beta1,6 N-acetylglucosaminyltransferase (C2GnT1) in leukocytes greatly increases their ...
... ions for the core 1 O-glycan of m/z 384.1, sulfated core 1 of m/z 464.1, sialylated core 1 of m/z 675.2, and disialylated core ... method was developed to differentiate and relatively quantitate the core 1 O-glycan and the sulfated core 1 O-glycan Gal- and ... Identification of Sulfation of Core-1 O-Glycan in Saliva. Given the identification of the sulfated core 1 O-glycans as an ... The acute ReA O-glycans contained all possible isomers of the sulfated core 1 O-glycan with the sulfate on the GalNAc and on ...
... and the glycans fucosyl-α-1,6-N,N′-diacetylchitobiose (N2F N-glycan), which forms part of the core fucosylation; fucosyl-α-1,6- ... Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine. Jimmy ... Core fucosylation of maternal milk N-glycan evokes B cell activation by selectively promoting the L-fucose metabolism of gut ... Unique Microbial Catabolic Pathway for the Human Core N-Glycan Constituent Fucosyl-α-1,6-N-Acetylglucosamine-Asparagine ...
Core-fucosylated N-Glycan,Labeled Core-fucosylated N-Glycan,Separation of Labeled Core-fucosylated N-Glycan,MS / HPLC Data of ... Core-fucosylated N-Glycan and Labeled Core-fucosylated N-Glycan based on Chemical Synthesis, ... Labeled Core-fucosylated N-Glycan,NMR Data of Labeled Core-fucosylated N-Glycan,Products,Related Products,This research is ... Core-fucosylated N-Glycan and Labeled Core-fucosylated N-Glycan based on Chemical Synthesis, ...
Galactosyltransferase-dependent sialylation of complex and endo-N-acetylglucosaminidase H-treated core N-glycans in vitro. ... Galactosyltransferase-dependent sialylation of complex and endo-N-acetylglucosaminidase H-treated core N-glycans in vitro. FEBS ... sialyltransferase were used to elongate and terminate glycan chains of agalacto-ovalbumin and endo-beta-N-acetylglucosaminidase ... sialyltransferase were used to elongate and terminate glycan chains of agalacto-ovalbumin and endo-beta-N-acetylglucosaminidase ...
The mucinous domain of pancreatic carboxyl-ester lipase (CEL) contains core 1/core 2 O-glycans that can be modified by ABO ... We found that the O-glycome of CEL consisted mainly of core 1/core 2 structures with a composition depending on the subjects ... Testing of the mAb16D10 antibody in glycan microarrays then demonstrated that it recognized structures containing terminal ... we used high-sensitivity MALDI-TOF MS to characterize the released O-glycan pool ofCELimmunoprecipitatedfromhumanpancreatic ...
You are here: Home / Publications / Aberrant expression of mucin core proteins and o-linked glycans associated with progression ... Aberrant expression of mucin core proteins and o-linked glycans associated with progression of pancreatic cancer. Authors: * ... We investigated the expression of a number of mucin core proteins and associated O-linked glycans expressed in pancreatic ... Immunohistochemical analyses of mucin and associated glycan expression on primary tumor and liver metastatic tumor samples were ...
Core 3 glycan is one of 4 core structures of O-GalNAc glycans of mucins. Core 3 β1,3-N-acetylglucosaminyltransferase (C3GnT) ... Core Glycan: Core 3 glycan is one of 4 core structures of O-GalNAc glycans of mucins. Core 3 β1,3-N- ... controls the biosynthesis of core 3 O-glycans and is expressed mainly in the intestine and salivary glands. Core 3 disaccharide ... can be further extended to form larger glycans. Reduced core3 synthase activity has been detected in malignant tumor cells and ...
Glycan analysis just got easier and more sensitive. This core kit includes the GlycoWorks Deglycosylation Module, GlycoWorks ... With just 3 easy steps in as little as 30 minutes, the GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time-consuming ... The RapiFluor-MS Intact mAb Mass Check Standard contains RapiFluor-MS labeled, released N-glycans obtained from 30 µg of Intact ... Product Listing: Glycoworks RapiFluor-MS N-Glycan Kit-96 sample - 176003606 to ACQUITY UPLC Glycoprotein BEH Amide Column, 300Å ...
... but not N565-glycan increases affinity of BoNT/A for human gSV2C-LD4. The N559-glycan was characterised as a complex core- ... The masses of N-glycans range from 892 Da of the common core structure subunit GlcNAc2Man3 to ,3500 Da of a complex sialylated ... N559 comprises a tetra-antennary, core-fucosylated N-glycan with a bisecting GlcNAc. The gSV2CLD-Fc N559Q and gSV2CLD-Fc N565Q ... Both sites carry core-fucosylated N-glycans ranging from dual- (∼15%), tri- (∼39%) to tetra-antennary (∼45%) structures of the ...
N-glycans,O-glycans, Mass Spectrometry Profile, Carbohydrate,Glycobiology,Glycoprotein,Gycopeptide,Glycolipid,Functional ... Consult with the Glycomics Core team to move your glycomics project(s) forward. The Glycomics Core can provide assistance with ... inactivated bacteria or virus on various glycan arrays including CFG glycan array, microbial glycan array, NCFG-derived defined ... N-glycan and O-glycan profiling of glycoproteins - individual glycoproteins, serum, cells or tissues by MALDI- (Matrix-Assisted ...
We discuss the bottle-neck and new opportunities in exploiting the chemo-enzymatic synthesis of N-glycans based on our research ... We discuss the bottle-neck and new opportunities in exploiting the chemo-enzymatic synthesis of N-glycans based on our research ... This review summarizes some recent advances in the chemo-enzymatic methods for the production of N-glycans, including the ... This review summarizes some recent advances in the chemo-enzymatic methods for the production of N-glycans, including the ...
Create a Sphingolipid Glycan Structure. Core:. Ceramide. R. Core chain style:. Straight. Bent. ... Glycan chain orientation:. Left. Right. Usage Sugar residues allowed:. Glc, Gal, Man, GlcNAc, GalNAc, Xyl, Fuc, NeuAc, NeuGc, ... Glycan sequence must be in the format:. [sugar (as an abbreviation)][anomer (either a or b)][linkage in (x-y form)]. ... Branched glycans are designated by parentheses:. GalNAca1-3GalNAcb1-3(Galb1-3GalNAcb1-4)Gala1-4Galb1-4Glcb. ...
Core Concept: Seismic tomography uses earthquake waves to probe the inner Earth ... Red meat-derived glycan promotes cancer. Annie N. Samraj, Oliver M. T. Pearce, Heinz Läubli, Alyssa N. Crittenden, Anne K. ... Red meat-derived glycan promotes cancer. Annie N. Samraj, Oliver M. T. Pearce, Heinz Läubli, Alyssa N. Crittenden, Anne K. ... A red meat-derived glycan promotes inflammation and cancer progression. Annie N. Samraj, Oliver M. T. Pearce, Heinz Läubli, ...
Depending on the sugars added, there are four common O-glycan core structures, cores 1 through 4, and an additional four, cores ... O-glycans are a class of glycans that modify serine or threonine residues of proteins. Biosynthesis of O-glycans starts from ... Mucin O-glycans can be branched, and many sugars or groups of sugars are antigenic. Important modifications of mucin O-glycans ... Mucin type O-glycan biosynthesis - Reference pathway [ Pathway menu , Pathway entry , Show description , Image (png) file , ...
Core Concept: The rise of bioelectric medicine sparks interest among researchers, patients, and industry ... Altered glycan-dependent signaling induces insulin resistance and hyperleptinemia. Donald A. McClain, William A. Lubas, Robert ... Altered glycan-dependent signaling induces insulin resistance and hyperleptinemia. Donald A. McClain, William A. Lubas, Robert ... Altered glycan-dependent signaling induces insulin resistance and hyperleptinemia Message Subject (Your Name) has sent you a ...
E-F) Env conserved core (black) with structural variation of Cα , 1.5 Å highlighted in red. (G) Residue-level schematic of ... C) Glycan residue-level details for glycan-glycan interactions. Glycans are shown in stick representation, with 2Fo-Fc electron ... 2Fo-Fc electron density (slate blue) is shown at 0.8 σ for glycans; assuming the average glycan is Man-7, ~50% of the glycan ... B) Features of crowded, dispersed and glycan-free surfaces. Correlations of crowded and dispersed glycans with glycan ...
Glycan metabolism * Glycan biosynthesis * M00872 O-glycan biosynthesis, mannose type (core M3) ... Defects of genes encoding core glycans and modified core M3 glycans are associated with various congenital diseases, such as ... to generate core M1, M2 and M3 glycans. Core M1 and M2 glycans can then be further attached by fucose residues, sialic acid ... Core M3 glycan is involved in the synthesis of alpha-dystroglycan, a heavily glycosylated protein found in muscle and brain ...
N2 - HMMC-1 is a human monoclonal antibody that reacts with a fucosylated and extended core 1 O-glycan, Fucα1-2Galβ1-4GlcNAcβ1- ... AB - HMMC-1 is a human monoclonal antibody that reacts with a fucosylated and extended core 1 O-glycan, Fucα1-2Galβ1-4GlcNAcβ1- ... HMMC-1 is a human monoclonal antibody that reacts with a fucosylated and extended core 1 O-glycan, Fucα1-2Galβ1-4GlcNAcβ1-3Gal ... HMMC-1, a human monoclonal antibody to fucosylated core 1 O-glycan, suppresses growth of uterine endometrial cancer cells. In: ...
The Glycomics Core in Boston provides services, instrumentation and expertise in glycomics to BIDMC research groups, affiliated ... The services proposed by the Glycomics Core includes:*Glycan Microarrays*Glycan analysis by Mass Spectrometry*Distribution of ... More Within Glycomics Core Glycomics Core* Services * Getting Started * Fees * Equipment * Related Links * Location * Contact ... These are the CFG Glycan Microarray version 5.2, and the Microbial Glycan Microarray (MGM) (also previously termed the Pathogen ...
The remaining trimannosyldiacetylchitobiose core structures can be removed with Endoglycosidase F3.. For more information on ... Asdiscussed previously,complex oligosaccharides can be reduced to the trimannosyldiacetylchitobiose (Man3GlcNAc2) core using ... Native and Sequential N-linked Glycan Strategies ...
6-fucose linked to core N-acetylglucosamine [GlcNAc]) of N-linked glycans in LAD II fibroblasts. The results showed that, ... The aim of this study was to determine the effects of a limited supply of GDP-L-fucose inside the Golgi on core fucosylation ( ... In particular, core fucosylation was found to be nearly absent in biantennary negatively charged oligosaccharides, whereas ... in core fucosylation observed in some species of oligosaccharides could also provide clues for the identification of glycans ...
The mutated enzyme specifically bound to the core of N-glycans, Man3GlcNAc2, across 600 glycans in a mammalian glycan array ( ... EndoBI-1 acted on the chitobiose core of N-glycans, probably leaving a GlcNAc residue (and an α1-6 fucose in core fucosylated ... A Mutant of EndoBI-1 Binds the Core of N-linked Glycans. The conserved active site of GH18 enzymes includes the motif DXE where ... hLF contains core fucosylated complex N-glycans, predominantly at two glycosylation sites (42). bLF represents a minor fraction ...
Glycan & Glycopeptide. * RapiFluor labeled glycan identification and quantification of released N-glycans by HILIC UPLC-FLR/MSE ... KBIs state-of-the-art mass spectrometry core facility delivers unparalleled structural characterization services to our ... Site specific glycan profiling of fully glycosylated & reduced peptide map by HILIC-UPLC-UV/MSE ... Glycan site occupancy from deglycosylated & reduced peptide map by RP-UPLC-UV/MSE ...
Structural analysis of murine zona pellucida glycans. Evidence for the expression of core 2-type O-glycans and the Sda antigen ... structural considerations suggest that core fucosylation of the essential N-glycan that immediately precedes the intermolecular ... The N-glycan attached to conserved N65 and the C66 cross-link are shown as sticks. b-f Details of the asymmetric and symmetric ... f, g Detailed views of the N65-linked glycans of Endo H-treated (panel f) and glycosylated cZP1-N1 (panel g). Electron density ...
Furthermore, loss of core 1-derived O-glycans induced inverse shifts in the abundance of the phyla Bacteroidetes and Firmicutes ... Here we used TM-IEC C1galt (-/-) mice, which carry an inducible deficiency of core 1-derived O-glycans in intestinal epithelial ... Altered mucus glycosylation in core 1 O-glycan-deficient mice affects microbiota composition and intestinal architecture.. ...
The N-linked core oligosaccharide. N-linked glycans are added to proteins in the ER as "core oligosaccharides" that have the ... the glycans do not display such heterogeneity. The "core glycans" are homogeneous and relatively simple (Fig. 1). ... Of the original core glycan, just five sugars remain. Only one of many possible terminal glycosylation pathways is shown; the ... When two of the glucoses in the N-linked core glycans have been trimmed away by glucosidases I and II, the nascent or newly ...
Loss of intestinal core 1-derived O-glycans causes spontaneous colitis in mice.. Artikel i vetenskaplig tidskrift ... These data indicate a causal role for the loss of core 1-derived O-glycans in colitis. Finally, we detected a biosynthetic ... Here, we report that mice with intestinal epithelial cell-specific deficiency of core 1-derived O-glycans, the predominant form ... Furthermore, induced deletion of intestinal core 1-derived O-glycans caused spontaneous colitis in adult mice. ...
O-glycan biosynthesis, mucin type core, organism-specific biosystem (from KEGG) O-glycan biosynthesis, mucin type core, ... O-glycan biosynthesis, mucin type core, conserved biosystem (from KEGG) O-glycan biosynthesis, mucin type core, conserved ... mucin core 1 and core 2 O-glycosylation, organism-specific biosystem (from BIOCYC) mucin core 1 and core 2 O-glycosylation, ... Mucin type O-glycan biosynthesis, organism-specific biosystemO-glycans are a class of glycans that modify serine or threonine ...
  • These exoglycosidases can be used to remove a terminal sugar residue, to determine the sequence of a glycan, or for modifying glycans on glycoproteins. (neb.com)
  • Mgat5 (mannoside acetylglucosaminyltransferase 5)-modified N-glycans, formed in the medial Golgi, promote structural heterogeneity in glycoproteins that regulate cellular interactions by binding to galectins. (jacksonmealsmatter.com)
  • Core 1 beta1,3-galactosyltransferase 1, generates the core T antigen, which is a precursor for many extended O-glycans in glycoproteins and plays a central role in many processes, such as angiogenesis, thrombopoiesis and kidney homeostasis development. (illumina.com)
  • Glycation is the result of covalent binding of a peptide, protein or lipid molecule with a glycan molecule without the controlling action of an enzyme (compared to glycosylation, which includes the controlling action of an enzyme). (dc4u-technologies.nl)
  • Representations of glycosylation characteristics are given in terms of Agal (agalactosylation), Monogal (monogalactosylation), Digal (digalactosylation), Sial (sialylation), Bisec (bisecting GlcNAc), Fuc (core-fucosylation). (harrisbee.org)
  • Co-expression of γ2 subunits hinders processing of N-linked glycans attached to the N104 glycosylation sites of GABAA receptor β2 subunits. (vanderbilt.edu)
  • The saccharide side-chain of a protein, known as a glycan, is thought to be important in maintaining protein structure, activity and folding, but little is known about its role. (vikkicantrill.com)
  • They found that in a protein from a human immune cell receptor, the core is responsible for increasing the rate of folding and affects its stability. (vikkicantrill.com)
  • When all of the units are present, the researchers observed that the rate of glycan folding increases 4-fold and the structure is stabilized significantly - by 3.1 kcal/mol - compared to a protein with no core attached. (vikkicantrill.com)
  • This result shows the importance of conserved saccharide cores and sheds light on potential general mechanisms for protein folding. (vikkicantrill.com)
  • Analysis of these glycans can be accomplished with the use of deglycosylation enzymes, which can provide complete sugar removal with no protein degradation. (neb.com)
  • Proteoglycans consist of a core protein covalently linked to one or more glycosaminoglycan (GAG) chains (for review, observe ref. 17). (tg-101348.info)
  • Using engineered presequence probes, photo cross-linking sites on mitochondrial proteins were mapped mass spectrometrically, thereby defining a presequence-binding domain of Tim50, a core subunit of the TIM23 complex that is essential for mitochondrial protein import. (jpt.com)
  • As with other molecules, like proteins, visualization of the 3D structures of glycans adds valuable information for understanding their biological function. (crip-power.com)
  • One important type of molecule on the cell's surface is the glycans-long carbohydrate molecules that come in phenomenal variety. (blogspot.com)
  • We have identified glycans that can be covalently linked to antigens to target specific DC receptors with the purpose to boost the immune response. (dc4u-technologies.nl)
  • Our GlycoDC TM technology can either potentiate or suppress the immune system via specific targeting of DC receptors by these glycan modified antigen conjugates. (dc4u-technologies.nl)
  • Different glycans target specific but different DC receptors, leading to completely different DC processing, antigen presentation and T-cell response. (dc4u-technologies.nl)
  • In this way our dendritic cell targeting technology enables opportunities for both immune stimulation and suppression by selection of the right combination of glycan and antigen / allergen. (dc4u-technologies.nl)
  • DC specific targeting: Glycated antigens target glycan-specific DC receptors, leading to specific DC internalization and antigen presentation. (dc4u-technologies.nl)
  • Glycan-antigens target DC receptors, resulting in DC internalization, loading of peptide antigen on both MHC-I and MHC-II to present to both CD8+ and CD8+T cells. (dc4u-technologies.nl)
  • The hypersialylation of core 1 O-glycans is due to the increase of NeuAcα2,6-containing structures (mainly NeuAcα2-3Galβ1-3[NeuAcα2-6]GalNAc). (elsevier.com)
  • She discovered that some DC receptors have a high specificity for binding specific glycan structures. (dc4u-technologies.nl)
  • We have demonstrated in several national and international research projects that glycan structures can be used as probes to directly target DCs in tissue and thereby trigger and modulate a specific immune response. (dc4u-technologies.nl)
  • N-Acetylglucosaminyltransferase I (GnT-I), which catalyzes the transfer of an N-acetylglucosamine residue from UDP-N-acetylglucosamine to the α1,3-linked mannose on Man 5 GlcNAc 2 (M5), is a critical enzyme for the synthesis of high-mannose-type to complex-type glycan structures in N-linked glycan developed a large-scale preparation system for recombinant human. (jacksonmealsmatter.com)
  • Trimeric HIV-1-Env Structures Define Glycan Shields from Clades A, B, and G. (vanderbilt.edu)
  • This study addresses fibroblast CMP-NeuAc levels and N- and O-glycan sialylation of serum proteins from Sialuria patients. (elsevier.com)
  • Isoforms of these proteins can be used as specific markers for the biosynthesis of N- and core 1 O-glycans. (elsevier.com)
  • Furthermore, total N- and O-linked glycans from serum proteins were analyzed by HPLC. (elsevier.com)
  • Bioseparations Columns & Consumables Chemistry consumables for the accurate analysis of peptides, proteins, oligonucleotides, glycans, and amino acids. (waters.com)
  • Glycan-based interactions involving vertebrate sialic-acid-recognizing proteins. (anthropogeny.org)
  • Now, a team led by Evan Powers and Jeffery Kelly at the Scripps Research Institute, USA, and Chi-Huey Wong at the Academia Sinica, Taiwan, has analyzed a well- defined and highly conserved saccharide core within a particular glycan 1 . (vikkicantrill.com)
  • Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. (neb.com)
  • Aminoquinoline fluorescent labels obstruct efficient removal of N-glycan corex Journal of Proteome Research . (neb.com)
  • Negatively charged red-emitting acridine dyes for facile reductive amination, separation and fluorescent detection of glycans. (mpg.de)
  • Surprisingly little is well known on the subject of the modulation of core endophenotypes of psychiatric disease simply by discrete noradrenergic (NE) circuits. (biotechnologysymposium.com)
  • We identify that BanLec recognizes a subset of high-mannose glycans via bidentate interactions spanning the two binding sites present on each BanLec monomer that were previously considered separate carbohydrate recognition domains. (diamond.ac.uk)
  • The core of our technology is glycation of antigens to which we direct the immune response. (dc4u-technologies.nl)
  • We have identified inhibitory glycans which can be linked to antigens with the purpose to suppress the immune response by instructing DCs to differentiate and activate regulatory T-cells (Tregs). (dc4u-technologies.nl)
  • α-mannoside β-1,6-N-acetylglucosaminyltransferase V (MGAT5) is a mammalian glycosyltransferase involved in complex N-glycan formation, which strongly drives cancer when overexpressed. (diamond.ac.uk)
  • GRC has also established critical facilities for cell biology and biomedical technology development, including novel mass spectrometry facility, NMR and X-ray facility for structure determination, microarray, peptide synthesis, high throughput genome sequencer and glycan analysis platforms for genomic research, and antibody development and optimization core, the animal laboratory, and Biosafety Level 3 Laboratory for pathological analysis and drug lead development. (sinica.edu.tw)
  • O-glycan analysis of natural human neutrophil gelatinase B using a combination of normal phase-HPLC and online tandem mass spectrometry: implications for the domain organization of the enzyme. (expasy.org)
  • Besides providing energy, sugars also exist as chains or 'glycans' that provide cellular and tissue structure and have major consequences on a cell's ability to survive, proliferate, migrate and/or exert its function. (fiu.edu)
  • For instance, though the term "lineage-specific biology," which is the exact opposite of evolutionary expectations, has been popular in recent years, it could have been used half a century ago for glycans when J.B.S.Haldane observed that "every species of mammal and bird so far investigated has shown quite a surprising biochemical diversity by serological tests. (blogspot.com)
  • Modification of these antigens by conjugation with a specific glycan structure enables specific targeting of certain DC receptors. (dc4u-technologies.nl)
  • Group has established a quality management system that conforms to DIN EN ISO 9001:2008 and which is integrated into daily practice. (labochema.com)
  • Complex N-glycan number and degree of branching cooperate to regulate cell proliferation and differentiation. (vanderbilt.edu)
  • Minor changes were found for serum N-glycans and hypersialylation was found for core 1 O-glycans on serum apoC-III and on total serum O-glycans in Sialuria patients. (elsevier.com)
  • Subsequently, increased amounts of α2,6-linked NeuAc were found on serum core 1 O-glycans from Sialuria patients. (elsevier.com)
  • We show that both sites are required for high-affinity glycan binding and virus neutralization. (diamond.ac.uk)
  • The TGIF provides a state-of-the-art research training environment, an Academic Oversight mentoring program and additional laboratory resources to all core glycoscience investigators and affiliate members. (fiu.edu)
  • In collaboration with the Institute Antiviral Research at Utah State, Gnubiotics has accelerated pre-clinical trials to explore the effects of its multivalent glycan formulations on live SARS-CoV-2 viruses in cell culture as well as evaluating the inhibition of viral induced cytopathic effects (CPE). (denoordstercreaties.nl)
  • The core attributes of the Coatbridge business remain exceptionally strong and it was recognised that the business is set up to achieve its ambitions under new ownership. (gilinvest.com)
  • Hinshaw, S. M. , and Harrison, S. C. (2013) An Iml3-Chl4 heterodimer links the core centromere to factors required for accurate chromosome segregation . (cornell.edu)
  • It is a venue for investigators, including non-glycoscience scientists, to share their glycobiological observations and efforts to advance their findings through collaborations with our core investigators and affiliate members. (fiu.edu)
  • Anxiety produced by withdrawal from repeated cocaine use can precipitate relapse to drug-seeking and drug-taking behaviors, and the prevention of relapse is at the core of efforts to treat drug addiction. (grantome.com)