Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.
A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
Myosin type II isoforms found in smooth muscle.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.
An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.
A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
The process of bone formation. Histogenesis of bone including ossification.
Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Factors that form a preinitiation complex at promoters that are specifically transcribed by RNA POLYMERASE I.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain.
Established cell cultures that have the potential to propagate indefinitely.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A ubiquitously expressed telomere-binding protein that is present at TELOMERES throughout the CELL CYCLE. It is a suppressor of telomere elongation and may be involved in stabilization of telomere length. It is structurally different from TELOMERIC REPEAT BINDING PROTEIN 2 in that it contains acidic N-terminal amino acid residues.
A family of transcription factors found primarily in PLANTS that bind to the G-box DNA sequence CACGTG or to a consensus sequence CANNTG.
A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.
Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.
DNA sequences encoding the delta chain of the T-cell receptor. The delta-chain locus is located entirely within the alpha-chain locus.
A strain of Murine leukemia virus (LEUKEMIA VIRUS, MURINE) arising during the propagation of S37 mouse sarcoma, and causing lymphoid leukemia in mice. It also infects rats and newborn hamsters. It is apparently transmitted to embryos in utero and to newborns through mother's milk.
Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.
Ordered rearrangement of T-cell variable gene regions coding for the delta-chain of antigen receptors.

FIRE3 in the promoter of the rat fatty acid synthase (FAS) gene binds the ubiquitous transcription factors CBF and USF but does not mediate an insulin response in a rat hepatoma cell line. (1/96)

Several putative insulin-responsive elements (IRE) in the fatty acid synthase (FAS) promoter have been identified and shown to be functional in adipocytes and hepatocytes. Here we report on the insulin-responsiveness in the rat hepatoma cell line H4IIE of four cis-elements in the FAS promoter: the FAS insulin-responsive elements, FIRE2 and FIRE3; the inverted CCAAT element, ICE; and the insulin/glucose-binding element, designated hepatic FIRE element, hFIRE, originally identified in rat hepatocytes. Using electrophoretic mobility shift assay (EMSA) competition experiments together with supershifts and in vitro transcription/translation we show that FIRE3 (-68/-58) binds not only the upstream stimulatory factors USF-1/USF-2 but also the CCAAT-binding factor CBF, also known as the nuclear factor Y, NF-Y. The putative IRE FIRE2, which shows sequence similarity to FIRE3, is located between -267 and -249. Gel retardation experiments indicate that USF-1 and USF-2 also bind to this element, which contains an imperfect E-box motif. Using the same approach we have shown that hFIRE binds the stimulatory proteins Sp1 and Sp3 in addition to CBF. Transient transfection experiments using FAS promoter constructs deleted for FIRE2 and FIRE3 demonstrate that neither of these elements mediates the insulin response of the FAS promoter in the rat hepatoma cell line H4IIE, however, ICE at -103/-87 is responsible for mediating the effect of the insulin antagonist cAMP. The hFIRE element located at -57/-34, in spite of its role in the glucose/insulin response in primary rat hepatocytes, is apparently not involved in the insulin regulation of the rat FAS promoter in H4IIE cells. The fact that the topology of the promoters of the FAS genes in rat, human, goose and chicken is conserved regarding CBF-binding sites and USF-binding sites implies an important role for these ubiquitously expressed transcription factors in the regulation of the FAS promoter.  (+info)

Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation. (2/96)

Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene family that is expressed at high levels in diverse human carcinomas and in articular cartilage from arthritic patients. In addition to its expression in pathological conditions, collagenase 3 has been detected in osteoblasts and hypertrophic chondrocytes during fetal ossification. In this work, we have evaluated the possibility that Cbfa1 (core binding factor 1), a transcription factor playing a major role in the expression of osteoblastic specific genes, is involved in the expression of collagenase 3 during bone formation. We have functionally characterized a Cbfa motif present in the promoter region of collagenase 3 gene and demonstrated, by cotransfection experiments and gel mobility shift assays, that this element is involved in the inducibility of the collagenase 3 promoter by Cbfa1 in osteoblastic and chondrocytic cells. Furthermore, overexpression of Cbfa1 in osteoblastic cells unable to produce collagenase 3 leads to the expression of this gene after stimulation with transforming growth factor beta. Finally, we show that mutant mice deficient in Cbfa1, lacking mature osteoblasts but containing hypertrophic chondrocytes which are also a major source of collagenase 3, do not express this protease during fetal development. These results provide in vivo evidence that collagenase 3 is a target of the transcriptional activator Cbfa1 in these cells. On the basis of these transcriptional regulation studies, together with the potent proteolytic activity of collagenase 3 on diverse collagenous and noncollagenous bone and cartilage components, we proposed that this enzyme may play a key role in the process of bone formation and remodeling.  (+info)

Does adult fracture repair recapitulate embryonic skeletal formation? (3/96)

Bone formation is a continuous process that begins during fetal development and persists throughout life as a remodeling process. In the event of injury, bones heal by generating new bone rather than scar tissue; thus, it can accurately be described as a regenerative process. To elucidate the extent to which fetal skeletal development and skeletal regeneration are similar, we performed a series of detailed expression analyses using a number of genes that regulate key stages of endochondral ossification. They included genes in the indian hedgehog (ihh) and core binding factor 1 (cbfa1) pathways, and genes associated with extracellular matrix remodeling and vascular invasion including vascular endothelial growth factor (VEGF) and matrix metalloproteinase 13 (mmp13). Our analyses suggested that even at the earliest stages of mesenchymal cell condensation, chondrocyte (ihh, cbfa1 and collagen type II-positive) and perichondrial (gli1 and osteocalcin-positive) cell populations were already specified. As chondrocytes matured, they continued to express cbfa1 and ihh whereas cbfa1, osteocalcin and gli1 persisted in presumptive periosteal cells. Later, VEGF and mmp13 transcripts were abundant in chondrocytes as they underwent hypertrophy and terminal differentiation. Based on these expression patterns and available genetic data, we propose a model where Ihh and Cbfa1, together with Gli1 and Osteocalcin participate in establishing reciprocal signal site of injury. The persistence of cbfa1 and ihh, and their targets osteocalcin and gli1, in the callus suggests comparable processes of chondrocyte maturation and specification of a neo-perichondrium occur following injury. VEGF and mmp13 are expressed during the later stages of healing, coincident with the onset of vascularization of the callus and subsequent ossification. Taken together, these data suggest the genetic mechanisms regulating fetal skeletogenesis also regulate adult skeletal regeneration, and point to important regulators of angiogenesis and ossification in bone regeneration.  (+info)

Mutation analysis of core binding factor A1 in patients with cleidocranial dysplasia. (4/96)

Cleidocranial dysplasia (CCD) is a dominantly inherited disorder characterized by patent fontanelles, wide cranial sutures, hypoplasia of clavicles, short stature, supernumerary teeth, and other skeletal anomalies. We recently demonstrated that mutations in the transcription factor CBFA1, on chromosome 6p21, are associated with CCD. We have now analyzed the CBFA1 gene in 42 unrelated patients with CCD. In 18 patients, mutations were detected in the coding region of the CBFA1 gene, including 8 frameshift, 2 nonsense, and 9 missense mutations, as well as 2 novel polymorphisms. A cluster of missense mutations at arginine 225 (R225) identifies this residue as crucial for CBFA1 function. In vitro green fluorescent protein fusion studies show that R225 mutations interfere with nuclear accumulation of CBFA1 protein. There is no phenotypic difference between patients with deletions or frameshifts and those with other intragenic mutations, suggesting that CCD is generally caused by haploinsufficiency. However, we were able to extend the CCD phenotypic spectrum. A missense mutation identified in one family with supernumerary teeth and a radiologically normal skeleton indicates that mutations in CBFA1 can be associated exclusively with a dental phenotype. In addition, one patient with severe CCD and a frameshift mutation in codon 402 had osteoporosis leading to recurrent bone fractures and scoliosis, providing first evidence that CBFA1 may help maintain adult bone, in addition to its function in bone development.  (+info)

A full-length Cbfa1 gene product perturbs T-cell development and promotes lymphomagenesis in synergy with myc. (5/96)

The Cbfa1/PEBP2 alpha A/AML3 gene plays an essential role in osteogenesis but is also expressed in the T-cell lineage where it has been implicated in lymphoma development as a target for retroviral insertional mutagenesis. As lymphoma cells with til-1 insertion express at least five distinct Cbfa1 isoforms, it is important to establish which, if any, have intrinsic oncogenic potential. We have generated transgenic mice in which the most abundant lymphoma isoform (G1/p57) is expressed under the control of the CD2 locus control region. Co-precipitation analysis of transgenic thymus revealed high levels of Cbfa1 protein in an abundant complex containing the binding cofactor Cbfb. CD2-Cbfa1-G1 mice displayed abnormal T-cell development, with a pronounced skew towards CD8 SP cells in the thymus and developed a low incidence of spontaneous lymphomas (6% at 12 months) with cells of similar phenotype. Strongly synergistic tumour development was seen when CD2-Cbfa1-G1 mice were crossed with lines carrying myc transgenes (CD2-myc or tamoxifen-regulatable CD2-mycER) and Cbfa1 was found to rescue expression of the CD2-myc transgene in pre-leukaemic mice. However, synergy did not appear to be due to a dominant block of myc-induced apoptosis by Cbfa1 as explanted primary tumours and cell lines from CD2-Cbfa1-G1/CD2-mycER mice showed accelerated death on induction with tamoxifen at similar rates to CD2-mycER controls. Moreover, thymocytes from preleukaemic CD2-Cbfa1-G1 mice showed reduced survival in vitro and increased sensitivity to the inhibitory effects of TGF-beta. This study demonstrates that a full-length Cbf alpha-chain gene can act as an oncogene without fusion to a heterologous protein.  (+info)

Parathyroid hormone regulation of the rat collagenase-3 promoter by protein kinase A-dependent transactivation of core binding factor alpha1. (6/96)

Previously we showed that the activator protein-1 site and the runt domain binding site in the collagenase-3 promoter act cooperatively in response to parathyroid hormone (PTH) in the rat osteoblastic osteosarcoma cell line, UMR 106-01. Our results of the expression pattern of core binding factor alpha1 (Cbfa1), which binds to the runt domain site, indicated that there is no change in the levels of Cbfa1 protein or RNA under either control conditions or after PTH treatment. The importance of posttranslational modification of Cbfa1 in the signaling pathway for PTH-induced collagenase-3 promoter activity was analyzed. PTH stimulation of collagenase-3 promoter activity was completely abrogated by protein kinase A (PKA) inhibition. To determine the role of PKA activity with respect to Cbfa1 activation (in addition to its known activity of phosphorylating cAMP-response element-binding protein to enhance c-fos promoter activity), we utilized the heterologous Gal4 transcription system. PTH stimulated the transactivation of activation domain-3 in Cbfa1 through the PKA site. In vitro phosphorylation studies indicated that the PKA site in the wild type activation domain-3 is a substrate for phosphorylation by PKA. Thus, we demonstrate that PTH induces a PKA-dependent transactivation of Cbfa1, and this transactivation is required for collagenase-3 promoter activity in UMR cells.  (+info)

An osteogenesis-related transcription factor, core-binding factor A1, is constitutively expressed in the chondrocytic cell line TC6, and its expression is upregulated by bone morphogenetic protein-2. (7/96)

Core-binding factor A1 (Cbfa1), also called Pebp2 alpha A/AML3, is a transcription factor that belongs to the runt-domain gene family. Cbfa1-deficient mice are completely incapable of both endochondral and intramembranous bone formation, indicating that Cbfa1 is indispensable for osteogenesis. Maturation of chondrocytes in these mice is also disorganized, suggesting that Cbfa1 may also play a role in chondrogenesis. The aim of this study was to examine the expression and regulation of Pebp2 alpha A/AML3/Cbfa1 expression in the chondrocyte-like cell line, TC6. Northern blot analysis indicated that Cbfa1 mRNA was constitutively expressed as a 6.3 kb message in TC6 cells and the level of Cbfa1 expression was enhanced by treatment with bone morphogenetic protein-2 (BMP2) in a time- and dose-dependent manner. This effect was blocked by an RNA polymerase inhibitor, 5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole, but not by a protein synthesis inhibitor, cycloheximide. Western blot analysis of the cell lysates using polyclonal antibody raised against Cbfa1 indicated that BMP2 treatment increased the Cbfa1 protein level in TC6 cells. In TC6 cells, BMP2 treatment enhanced expression of alkaline phosphatase and type I collagen mRNAs but suppressed that of type II collagen mRNA. In addition to TC6 cells, Cbfa1 mRNA was also expressed in primary cultures of chondrocytes and BMP2 treatment enhanced Cbfa1 mRNA expression in these cells similarly to its effect on TC6 cells. These data indicate that the Pebp2 alpha A/AML3/Cbfa1 gene is expressed in a chondrocyte-like cell line, TC6, and its expression is enhanced by treatment with BMP.  (+info)

Exogenous cdk4 overcomes reduced cdk4 RNA and inhibition of G1 progression in hematopoietic cells expressing a dominant-negative CBF - a model for overcoming inhibition of proliferation by CBF oncoproteins. (8/96)

Core Binding Factor (CBF) is required for the development of definitive hematopoiesis, and the CBF oncoproteins AML1-ETO, TEL-AML1, and CBFbeta-SMMHC are commonly expressed in subsets of acute leukemia. CBFbeta-SMMHC slows the G1 to S cell cycle transition in hematopoietic cells, but the mechanism of this effect is uncertain. We have sought to determine whether inhibition of CBF-mediated trans-activation is sufficient to slow proliferation. We demonstrate that activation of KRAB-AML1-ER, a protein containing the AML1 DNA-binding domain, the KRAB repression domain, and the Estrogen receptor ligand binding domain, also slows G1, if its DNA-binding domain is intact. Also, exogenous AML1 overcame CBFbeta-SMMHC-induced inhibition of proliferation. Representational difference analysis (RDA) identified cdk4 RNA expression as an early target of KRAB-AML1 activation. Inhibition of CBF activities by KRAB-AML1-ER or CBFbeta-SMMHC rapidly reduced endogenous cdk4 mRNA levels, even in cells proliferating at or near control rates as a result of exogenous cdk4 expression. Over-expression of cdk4, especially a variant which cannot bind p16INK4a, overcame cell cycle inhibition resulting from activation of KRAB-AML1-ER, although cdk4 did not accelerate proliferation when expressed alone. These findings indicate that mutations which alter the expression of G1 regulatory proteins can overcome inhibition of proliferation by CBF oncoproteins. Oncogene (2000).  (+info)

TY - JOUR. T1 - Combination of dasatinib with chemotherapy in previously untreated core binding factor acute myeloid leukemia. T2 - CALGB 10801. AU - Marcucci, Guido. AU - Geyer, Susan. AU - Laumann, Kristina. AU - Zhao, Weiqiang. AU - Bucci, Donna. AU - Uy, Geoffrey L.. AU - Blum, William. AU - Eisfeld, Ann Kathrin. AU - Pardee, Timothy S.. AU - Wang, Eunice S.. AU - Stock, Wendy. AU - Kolitz, Jonathan E.. AU - Kohlschmidt, Jessica. AU - Mrózek, Krzysztof. AU - Bloomfield, Clara D.. AU - Stone, Richard M.. AU - Larson, Richard A.. N1 - Publisher Copyright: © 2020 by The American Society of Hematology. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2020/2/25. Y1 - 2020/2/25. N2 - Acutemyeloid leukemia (AML)witheither t(8;21)(q22;q22)or inv(16)(p13q22)/t(16;16)(p13;q22) is referred to as core binding factor (CBF) AML. Although categorized as favorable risk, long-term survival for these patients is only ∼50% to 60%. Mutated (mut) or overexpressed KIT, a gene encoding a ...
Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. Few prognostic markers have been identified. In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. Overall, 10-year overall survival (OS), disease-free survival (DFS), and event-free survival were 63.9%, 54.8%, and 49.9%, respectively; patients with the t(8;21) and inv(16) chromosomal rearrangements exhibited significant differences at diagnosis. Despite similarly high complete remission (CR) rate, patients with inv(16) experienced superior DFS and a high chance of achieving a second CR, often leading to prolonged OS also after relapse. We found that a complex karyotype (ie, ≥4 cytogenetic anomalies) affected survival; the KIT D816 mutation predicted worse prognosis only in patients with the t(8;21) rearrangement, whereas FLT3 mutations had no ...
Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
This study is examining the appropriate dose and side effects of dasatinib, when it is given with the standard of care chemotherapy for children and adolescents
KIT is a receptor tyrosine kinase that is functionally relevant for hematopoiesis, mast cell development and function, gametogenesis and melanogenesis. Normal KIT signaling requires binding to stem cell factor, and PI3K-Akt is one of the putative effector pathways. In humans, germline loss-of-function KIT mutations have been associated with piebaldism - an autosomal dominant condition characterized by depigmented patches of skin and hair. Gain-of-function KIT mutations are usually acquired and have been associated with myeloid malignancies including core binding factor acute myeloid leukemia and systemic mastocytosis (SM), germ cell tumors, gastrointestinal stromal tumors and sinonasal T cell lymphomas. KITD816V is the most prevalent KIT mutation in mast cell disease and occurs in more than 90% of the cases that fulfill the World Health Organization diagnostic criteria for SM. However, its precise pathogenetic contribution is not well understood. In clinical practice, SM is considered either indolent or
core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
TY - JOUR. T1 - Selection of reversions and suppressors of a mutation in the CBF binding site of a lymphomagenic retrovirus. AU - Martiney, Marita J.. AU - Rulli, Karen. AU - Beaty, Robert. AU - Levy, Laura S.. AU - Lenz, Jack. PY - 1999/8/23. Y1 - 1999/8/23. N2 - The retrovirus SL3 induces T-cell lymphomas in mice. The transcriptional enhancer in the long terminal repeat (LTR) of SL3 contains two 72-bp repeats. Each repeat contains a binding site for the transcription factor CBF (also called AML1). The CBF binding sites are called core elements. SAA is a mutant that is identical to SL3 except for the presence of a single-base-pair substitution in each of the two core elements. This mutation significantly attenuates viral lymphomagenicity. Most lymphomas that occur in SAA-infected mice contain proviruses with reversions or second-site suppressor mutations within the core element. We examined the selective pressures that might account for the predominance of the reversions and suppressor ...
The purpose of the study is to determine whether Fludarabine in combination with cytarabine is more effective than high-dose cytarabine in post-remission
Acute myeloid leukemia 2 protein antibody, Acute myeloid leukemia gene 2 antibody, AML 2 antibody, AML2 antibody, CBF alpha 3 antibody, CBF-alpha-3 antibody, CBFA 3 antibody, CBFA3 antibody, Core binding factor alpha 3 subunit antibody, core binding factor antibody, Core binding factor runt domain alpha subunit 3 antibody, Core binding factor subunit alpha 3 antibody, core-binding factor antibody, Core-binding factor subunit alpha-3 antibody, FLJ34510 antibody, MGC16070 antibody, Oncogene AML 2 antibody, Oncogene AML-2 antibody, PEA2 alpha C antibody, PEA2-alpha C antibody, PEBP2 alpha C antibody, PEBP2-alpha C antibody, Pebp2a3 antibody, PEBP2aC antibody, Polyomavirus enhancer binding protein 2 alpha C subunit antibody, Polyomavirus enhancer-binding protein 2 alpha C subunit antibody, runt domain alpha subunit 3 antibody, runt related transcription factor 3 antibody, Runt-related transcription factor 3 antibody, RUNX 3 antibody, Runx3 antibody, RUNX3_HUMAN antibody, SL3 3 enhancer factor 1 ...
PRIMARY OBJECTIVES:. I. To assess the safety and tolerability of dasatinib with intensive induction therapy (daunorubicin hydrochloride and cytarabine), consolidation chemotherapy (high-dose cytarabine), and as single agent in maintenance therapy in patients with newly diagnosed core-binding factor acute myeloid leukemia (AML).. SECONDARY OBJECTIVES:. I. To assess clinical outcomes such as event-free survival (EFS), complete response (CR) rate, cumulative incidence of relapse (CIR), cumulative incidence of death (CID), disease-free survival (DFS), and overall survival (OS) of patients treated with these regimens.. II. To describe the frequency and severity of adverse events of patients treated on this study during induction, consolidation, and continuation therapy.. III. To describe the interaction of pretreatment disease and patient characteristics including morphology, cytogenetics, immunophenotype, molecular genetic features, white blood cell (WBC) count and hemogram, and performance status ...
Ustun C, Morgan E, Moodie EEM, Pullarkat S, Yeung C, Broesby-Olsen S, Ohgami R, Kim Y, Sperr W, Vestergaard H, Chen D, Kluin PM, Dolan M, Mrózek K, Czuchlewski D, Horny HP, George TI, Kristensen TK, Ku NK, Yi CA, Møller MB, Marcucci G, Baughn L, Schiefer AI, Hilberink JR, Pullarkat V, Shanley R, Kohlschmidt J, Coulombe J, Salhotra A, Soma L, Cho C, Linden MA, Akin C, Gotlib J, Hoermann G, Hornick J, Nakamura R, Deeg J, Bloomfield CD, Weisdorf D, Litzow MR, Valent P, Huls G, Perales MA, Borthakur G Core-binding factor acute myeloid leukemia with t(8;21): Risk factors and a novel scoring system (I-CBFit). View on PubMed ...
The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
Among patients with good prognosis core binding factor AML, there is an overall survival rate of only 44%. To understand the genetic factors contributing to poor outcomes within this subgroup, Dr Chew is analysing bone marrow samples collected from 18 patients before and during treatment.. According to Dr Chew, multiple genetic abnormalities acquired during therapy are probably responsible for good prognosis core binding AML developing resistance to chemotherapy.. To help us predict who will respond poorly to therapy, were identifying the genetic mutations occurring in patients who relapse, he said. This information will allow us to tailor patient treatment accordingly. Currently a stem cell transplant is considered the definitive treatment and our findings will help clinicians decide if their patients AML will develop resistance and if a stem cell transplant is recommended.. Dr Chew is testing the usefulness of the genetic variations he identifies through an international ...
Disease, Muscle, Muscle Cells, Smooth Muscle, Smooth Muscle Cells, Cells, Heme, Hydrogen, Alkaline Phosphatase, Kidney, Kidney Disease, Osteocalcin, Upregulation, Vascular Calcification, Calcium, Heme Oxygenase, Vascular Smooth Muscle, Atherosclerosis, Bone, Core Binding Factor
This phase II study of romidepsin in relapsed and refractory AML shows that this HDACI has a differential antileukemic activity in patients with CBF AML [predominantly t(8;21)] and that this was associated with an up-regulation of AML1 target genes. These findings are in keeping with observations in vitro that have established that t(8;21) AML is sensitive to therapy with various HDACIs including romidepsin (7, 16-19, 27). Despite the in vitro evidence suggesting an inherent sensitivity of this cytogenetic subset of AML to this class of agents, this is the first study to actually show antileukemic activity in vivo in a cohort of patients with t(8;21) AML and other translocations involving the AML1 gene.. Romidepsin has been investigated in a phase I trial (28) conducted at this dose and schedule in two groups of patients with chronic lymphocytic leukemia and AML, respectively. In that trial that involved 10 AML patients, there were no patients with CBF AML. There were no objective responses ...
Cells, Lead, Acute Myeloid Leukemia, Bone, Bone Marrow, Core-binding Factor, Cytogenetic, Cytogenetic Abnormalities, Disease, Leukemia, Marrow, Myeloid Leukemia, Patient, Patients, Protein Array, Proteins, Regression, Regression Analysis, Ability, Algorithm
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As per available reports about 248 journals, 14 Conferences and workshops are presently dedicated exclusively to Acute Respiratory Failure and about 1
To study the effects and importance of fluoride on FBs in the development of extraperiosteal calcification and the ossification of skeletal fluorosis, the presence of the osteogenic phenotype, which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in osteoblasts (OBs) exposed to fluoride was determined. Fibroblasts and osteoblasts were exposed to different concentrations of fluoride (0, 0.0001, 0.001, 0.1, 1.0, 10.0 and 20.0mg/L F-). By using RT-PCR and ELISA, the mRNA levels of Cbfa1 and OCN were measured at 48h, and the protein levels of Cbfa1 and OCN were measured at 2, 4, 24, 48 and 72h. The data demonstrated the following: (1) The Cbfa1 protein level in fluoride-treated fibroblasts clearly increased at 48h in the groups treated with 0.0001, 0.001, 0.1, 1.0 and 20.0mg/L F-. The Cbfa1 protein level of the group treated with 10mg/L F- at 72h was higher than that of the control group. The level of Cbfa1 mRNA in the fibroblasts ...
TY - JOUR. T1 - HDAC1 is a required cofactor of CBFb-SMMHC and a potential therapeutic target in inversion 16 acute myeloid leukemia. AU - Richter, Lisa E.. AU - Wang, Yiqian. AU - Becker, Michelle E.. AU - Coburn, Rachel A.. AU - Williams, Jacob T.. AU - Amador, Catalina. AU - Hyde, R. Katherine. PY - 2019/6/1. Y1 - 2019/6/1. N2 - Acute myeloid leukemia (AML) is a neoplastic disease characterized by the uncontrolled proliferation and accumulation of immature myeloid cells. A common mutation in AML is the inversion of chromosome 16 [inv (16)], which generates a fusion between the genes for core binding factor beta (CBFB) and smooth muscle myosin heavy chain gene (MYH11), forming the oncogene CBFB-MYH11. The expressed protein, CBFb-SMMHC, forms a heterodimer with the key hematopoietic transcription factor RUNX1. Although CBFb-SMMHC was previously thought to dominantly repress RUNX1, recent work suggests that CBFb-SMMHC functions together with RUNX1 to activate transcription of specific target ...
The novel FMS-like tyrosine kinase 3 (FLT3)-N676K point mutation within the FLT3 kinase domain-1 was recently identified in 6 % of de novo acute myeloid leukemia (AML) patients with inv(16). Because FLT3-N676K was encountered almost exclusively in inv(16) AML, we investigated the transforming potential of FLT3-N676K, the cooperation between FLT3-N676K and core binding factor ß-smooth muscle myosin heavy chain (CBFß-SMMHC) (encoded by the inv(16) chimeric gene CBFB-MYH11) in inducing acute leukemia, and tested the sensitivity of FLT3-N676K-positive leukemic cells to FLT3 inhibitors. Retroviral expression of FLT3-N676K in myeloid 32D cells induced AML in syngeneic C3H/HeJ mice (n = 11/13, median latency 58 days), with a transforming activity similar to FLT3-internal tandem duplication (ITD) (n = 8/8), FLT3-TKD D835Y (n = 8/9), and FLT3-ITD-N676K (n = 9/9) mutations. Three out of 14 (21.4 %) C57BL/6J mice transplanted with FLT3-N676K-transduced primary hematopoietic progenitor cells developed ...
Core binding factor (CBF) is a heterodimeric transcription factor that is essential for a number of developmental process including hemotopoiesis and bone development. CBFs contain a DNA-binding CBFα. subunit and a non-DNA binding CBFß. subunit ...
The pathogenesis of PDAC involves genetic alterations, such as K-ras protooncogene mutations, mutations of the p53, p16, and Smad4 tumour suppressor genes, and other less common mutations.2 In addition, there are numerous epigenetic alterations, including altered expression of several growth factors and their receptors.3 For example, PDACs overexpress all TGFβ isoforms and their receptors, and overexpression of these ligands and receptors is often associated with shortened postoperative survival of patients with pancreatic cancer.14,15. The TGFβ pathway is carefully regulated, with Smad proteins as the key component in the signal transduction pathway. In addition, other regulators, such as transcription factors, which facilitate Smad binding to target promoters, may provide routes for feedback and crosstalk.16 For example, members of the CBFA (core binding factor A) family of transcription factors act both as targets and partners of activated Smads. This family, also termed the Runx family, ...
According to Dr. Stone, MRD status in CBF-AML might also make a difference in treatment selection. A separate study of patients with CBF-AML found that those with an MRD level of at least 1% at one point following induction therapy experienced relapse 90% of the time, with a median remission duration of 10 months (P , .001).4 This is one group of patients we could justify transplanting, Dr. Stone observed, but that doesnt mean its going to make a difference.. MRD test results have also been shown to improve outcome prediction,5 albeit modestly, when included with more typical clinical data (ie, age, gender, performance status, white blood cell count, platelet count, bone marrow blast percentage, cytogenetic risk, and NPM1/FLT3-ITD status). Because predictive outcome was only improved by 3%, however, MRD testing is not worth the effort for that reason alone, according to Dr. Stone.. Testing Limitations. Whether performed with multiparameter flow cytometry or next-generation sequencing, MRD ...
Decreased Llgl1 expression has been previously shown to be associated with increased metastatic potential, malignant phenotype, or inferior survival in a variety of solid tumors (Ohali et al., 2004; Schimanski et al., 2005; Kuphal et al., 2006). Given that Llgl1 restrains self-renewal in HSCs and that leukemia is characterized by aberrant activation of self-renewal, we assessed for a role in human AML. First, we asked whether the gene expression signature revealed by genetic inactivation of Llgl1 (Llgl1−/− signature) in HSCs was associated with any genetic subtypes of primary AML. Indeed, clustering based on the Llgl1−/− signature (Fig. 4 B) grouped AML cases into subgroups significantly associated with cytogenetic abnormalities (Fig. 4, B and C). Especially core-binding factor leukemias (t(8;21) and inv(16)) or t(15;17) clustered into specific subgroups. Moreover, the Llgl1−/− signature was most strongly correlated with group 7 based on hierarchical clustering. This group contained ...
IMPACT Diagnostic International is a science-driven source for many of your testing needs. Our Southern California prepared media manufacturing facility, located in Pomona, exceeds the technical specifications and standards set by FDA c-GMP and NCCLS.. ...
Fingerprint Dive into the research topics of Identification of a region on the outer surface of the CBFβ-SMMHC myeloid oncoprotein assembly competence domain critical for multimerization. Together they form a unique fingerprint. ...
Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. Runx proteins are encoded by three genes: Runx1, Runx2 , and ...
This Phase I study is for children and adolescents who have acute myelogenous leukemia (AML) that has come back (relapsed) or has become resistant (refractory) to standard therapies. Researchers want to know if a drug called dasatinib is safe when used together with standard chemotherapy in treating patients who have relapsed or have resistant AML. Their leukemia has a particular genetic mutation, called core-binding factor. This type of leukemia has an increase of a cancer promoting protein called c-KIT. Dasatinib can target this protein in laboratory experiments. Laboratory and other studies suggest that dasatinib may prevent acute myeloid leukemia cells from growing and may lead to the destruction of leukemia cells. The main goal of this study is to find a safe dose of dasatinib and to find out the side effects of dasatinib when it is given in combination with standard chemotherapy to children and adolescents. Similar studies are currently being done in adult patients. Dasatinib has been ...
Complete information for CBFB gene (Protein Coding), Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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KIT gene mutations occur in approximately 25% of cases of acute myeloid leukemia (AML) with t(8;21) or inv(16), also referred to as core-binding factor AML. These mutations are predominately small length affecting mutations in KIT exon 8 and substitution mutations in KIT exon 17. KIT mutations in core-binding factor AML may be an adverse prognostic factor in this otherwise favorable-risk disease. This DNA sequencing test detects mutations in exons 8 and 17 of the KIT gene in blood and bone marrow specimens. This test will detect mutations in specimens when at least 30% of the nucleated cells carry the mutation.. ...
KIT tyrosine kinase is expressed in mast cells, interstitial cells of Cajal, and hematopoietic cells. Permanently active KIT mutations lead these host cells to tumorigenesis, and to such diseases as mast cell leukemia (MCL), gastrointestinal stromal tumor (GIST), and acute myeloid leukemia (AML). Recently, we reported that in MCL, KIT with mutations (D816V, human; D814Y, mouse) traffics to endolysosomes (EL), where it can then initiate oncogenic signaling. On the other hand, KIT mutants including KITD814Y in GIST accumulate on the Golgi, and from there, activate downstream. KIT mutations, such as N822K, have been found in 30% of core binding factor-AML (CBF-AML) patients. However, how the mutants are tyrosine-phosphorylated and where they activate downstream molecules remain unknown. Moreover, it is unclear whether a KIT mutant other than KITD816V in MCL is able to signal on EL. We used leukemia cell lines, such as Kasumi-1 (KITN822K, AML), SKNO-1 (KITN822K, AML), and HMC-1.1 (KITV560G, MCL), to explore
A series of studies of f-electron systems based on density functional theory methods have been performed. The focus of the studies has been on magnetic and structural properties, as well as investigating ways to handle strong electron correlation in these systems.. A version of the self-interaction correction (SIC) method has been developed for a full-potential linear muffin-tin orbital method. The method is demonstrated to have the strong capabilities of previous SIC implementations, to study energetics and phase stabilities of d- and f-electron systems with localisation-delocalisation transitions, but with no geometrical constraints from the underlying band structure method. The method is applied to the high-TC superconductor CeOFeAs, in which the f-shell of the Ce atoms is argued to undergo a Mott transition to a delocalised state under pressure.. The non-collinear magnetic structures of two rare earth compounds, TbNi5 and CeRhIn5 have been studied, and in both cases the complex magnetic ...
Saturday, October 17, 1953 ld Blinds The Daily Register-Mail, Galesburg, Til- Saturday, October 17, 1953 , 8 GALVA-Meeting of duck hunters of Lake Calhoun Association was held Thursday night at the clubhouse. Plans were made for those Interested to meet at 8 oclock Sunday morning at the clubhouse to build blinds for the hu. Edition of Galesburg Register-Mail
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The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two ...
Autor: Otto, Florian et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2002-02-13; Keywords: cleidocranial dysplasia; CCD; transcription factor; core binding factor; runt domain; RUNX2; CBFA1; differentiation; osteoblast; Titel: Mutations in the RUNX2 gene in patients with cleidocranial dysplasia
TY - JOUR. T1 - Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. AU - North, Trista E.. AU - De Bruijn, Marella F T R. AU - Stacy, Terryl. AU - Talebian, Laleh. AU - Lind, Evan. AU - Robin, Catherine. AU - Binder, Michael. AU - Dzierzak, Elaine. AU - Speck, Nancy A.. PY - 2002. Y1 - 2002. N2 - Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1+/- embryos are heterogeneous and include CD45+ cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs among these various cell populations is sensitive to Runx1 dosage. These data provide the first morphological description of ...
Only the PBM motif is a classic HLH motif. Three different ChIP-chip-derived motifs are all diverse, but all score highly on ChIP-chip data! Are they motifs of other TFs? Check. 602: GCN4; 1095, TEC1; 1096: resembles 602, but is a closer match to CUP9/TOS8. Also hits GCN4. According to the literature (PMID: 9032238) the core binding site for the Rtg1p-Rtg3p heterodimer is 5-GGTCAC-3; the only motif that resembles this is 1446. Vague resemblance to 602 and 1096. I am going to retain 1446, which represents the literature site; PBM motif 870, which resembles an E-box, and ChIP-chip motif 1445, which scores highest on ChIP-chip data. But give all low confidence ...
Runx1 mediates the development of the granular convoluted tubules in the submandibular glands[1] The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription factor (RUNX) proteins (RUNX1, 2 and 3). This result clearly demonstrates that the Runx /Cbfb signaling pathway is indispensable in the development of the GCTs. However, it is not clear which of the RUNX proteins plays useful role in the development of the GCTs by activating the Runx /Cbfb signaling pathway. Past studies have revealed that the Runx /Cbfb signaling pathway plays important roles in various aspects of development and homeostatic events. Moreover, the Runx genes have different temporospatial ...
View mouse Terf2ip Chr8:112011398-112020528 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Translokacje chromosomalne modyfikujące Core-Binding Factor[edytuj , edytuj kod]. Ostra białaczka mielobastyczna z cechami ... Core-binding factor acute myeloid leukemia.. „Arch Pathol Lab Med". 135 (11), s. 1504-9, Nov 2011. DOI: 10.5858/arpa.2010-0482- ... Core binding factor genes and human leukemia. „Haematologica". 87 (12), s. 1307-23, Dec 2002. PMID: 12495904. ... Core-binding factor acute myeloid leukemia: can we improve on HiDAC consolidation?. „Hematology Am Soc Hematol Educ Program". ...
Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... Core binding factor acute myeloid leukaemia is a cancer related to genetic changes in the CBF gene. It is most commonly caused ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... Hart SM, Foroni L (2003). "Core binding factor genes and human leukemia". Haematologica. 87 (12): 1307-23. PMID 12495904. Bae ...
... a DNA binding CBFα chain (RUNX1 or RUNX2) and a non-DNA-binding subunit called core binding factor β (CBFβ); the binding ... Wang, S, Speck, NA (January 1992). "Purification of core-binding factor, a protein that binds the conserved core site in murine ... Runt-related transcription factor 1 (RUNX1) also known as acute myeloid leukemia 1 protein (AML1) or core-binding factor ... It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). ...
RNA polymerase II core promoter proximal region sequence-specific DNA binding. • chromatin binding. • transcription factor ... transcription factor activity, sequence-specific DNA binding. • mitogen-activated protein kinase p38 binding. • FK506 binding. ... DNA binding. • RNA polymerase II regulatory region sequence-specific DNA binding. • RNA polymerase II transcription factor ... protein binding. • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding. • RNA polymerase ...
... is a nuclear protein that binds to DNA and acts as an architectural chromatin-binding factor. It can also be released ... Bernués J, Espel E, Querol E (May 1986). "Identification of the core-histone-binding domains of HMG1 and HMG2". Biochimica et ... After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in ... Contact with core histones changes the structure of nucleosomes. The presence of HMGB1 in the nucleus depends on ...
Protein CBFA2T3 (core-binding factor, runt domain, alpha subunit 2; translocated to, 3) is a protein that in humans is encoded ... "Entrez Gene: CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3". Hoogeveen AT, Rossetti S, ... Calabi F, Cilli V (Dec 1998). "CBFA2T1 (core-binding factor, runt domain, alpha subunit 2; translocated to, 3), a gene ... makes distinct contacts with multiple histone deacetylases and binds mSin3A through its oligomerization domain". Mol. Cell. ...
May 2016). "Comprehensive mutational profiling of core binding factor acute myeloid leukemia". Blood. 127 (20): 2451-9. doi: ... June 2011). "CCCTC-binding factor (CTCF) and cohesin influence the genomic architecture of the Igh locus and antisense ... August 2013). "Transcription factor binding in human cells occurs in dense clusters formed around cohesin anchor sites". Cell. ... February 2008). "Cohesin mediates transcriptional insulation by CCCTC-binding factor". Nature. 451 (7180): 796-801. Bibcode: ...
... core protein binds to the growth factors BMP-4 and influences its bioactivity. It has also been reported that the ... There is also evidence that biglycan binds to TGF-beta 1. Biglycan interacts with collagen, both via the core protein and GAG ... Bidanset DJ, Guidry C, Rosenberg LC, Choi HU, Timpl R, Hook M (March 1992). "Binding of the proteoglycan decorin to collagen ... The structure of biglycan core protein is highly conserved across species; over 90% homology has been reported for rat, mouse, ...
... factor binds to the core, forming the holoenzyme. After transcription starts, the factor can unbind and let the core enzyme ... In order to bind promoters, RNAP core associates with the transcription initiation factor sigma (σ) to form RNA polymerase ... RNA polymerase binding in bacteria involves the sigma factor recognizing the core promoter region containing the −35 and −10 ... A transcription factor and its associated transcription mediator complex must be attached to a DNA binding site called a ...
"Entrez Gene: CBFA2T2 core-binding factor, runt domain, alpha subunit 2; translocated to, 2". Rual JF, Venkatesan K, Hao T, ... The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several ...
Makde R, England J, Yennawar H, Tan S (2010). "Structure of the RCC1 chromatin factor bound to the nucleosome core particle". ... is attached to nucleosomes via core histones H2A and H2B. Thus, a gradient of GTP-bound Ran is generated around the vicinity of ... which directly binds the growing ends of microtubules and coordinates the binding of other +TIPs. Opposing the action of these ... Once they bind a kinetochore, they are stabilized and their dynamics are reduced. The newly mono-oriented chromosome oscillates ...
WRKY transcription factors have been a significant area of plant research for the past 20 years. The WRKY DNA-binding domain ... Eighteen amino acids are highly conserved in the WRKY protein domain, including the core motif, zinc-finger binding cysteines ... The WRKY domain is found in the WRKY transcription factor family, a class of transcription factors. The WRKY domain is found ... the evolutionary conserved DNA-binding specificities of WRKY transcription factors by molecular dynamics and in vitro binding ...
"Fibroblast growth factor-binding protein is a novel partner for perlecan protein core". J. Biol. Chem. 276 (13): 10263-71. doi: ... The heparan sulfate chains of perlecan bind growth factors in the ECM, and serve as co-ligands or ligand enhancers when bound ... Transcriptional activation by transforming growth factor-beta via a nuclear factor 1-binding element". J. Biol. Chem. 272 (8): ... "The protein core of the proteoglycan perlecan binds specifically to fibroblast growth factor-7". J. Biol. Chem. 275 (10): 7095- ...
"Fibroblast growth factor-binding protein is a novel partner for perlecan protein core". J Biol Chem. 276 (13): 10263-71. doi: ... Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. FGFBP1, or HBP17, binds ... 2000). "Induction of the angiogenic modulator fibroblast growth factor-binding protein by epidermal growth factor is mediated ... "Characterization and molecular cloning of a putative binding protein for heparin-binding growth factors". J Biol Chem. 266 (25 ...
... recombination activating gene 2 component is thought to form a six-bladed propeller in the active core that serves as a binding ... RAG2 plays a role of an accessory factor. Its primary function seems to be to interact with RAG1 protein and activate its ... Its N-terminal part is thought to form a six-bladed propeller in the active core. RAG2 is conserved among all species that ... RAG2 is one of the two core components of the RAG complex. RAG complex is a multiprotein complex that mediates the DNA cleavage ...
The dimeric eukaryotic upstream binding factor (UBF) binds the UCE and the core element. UBF recruits and binds a protein ... allowing the UCE and the core elements to come into contact. RRN3/TIF-IA is phosphorylated and binds Pol I. Pol I binds to the ... When rRNA synthesis is stimulated, SL1 (selectivity factor 1) will bind to the promoters of rDNA genes that were previously ... Clos, Joachim; Buttgereit, Detlev; Grummt, Ingrid (February 1986). "A purified transcription factor (TIF-IB) binds to essential ...
"DNA Binding and Phosphorylation Regulate the Core Structure of the NF-κB p50 Transcription Factor". Journal of the American ... phosphorylation represents a reversible switch that transmits intracellular signals in response to extracellular factors. Eyers ...
FFAR2 transcription is regulated by the XBP1 transcription factor which binds to the core promoter. Mouse studies utilizing ... Brown AJ, Jupe S, Briscoe CP (January 2005). "A family of fatty acid binding receptors". DNA and Cell Biology. 24 (1): 54-61. ...
2008). "Histone deacetylase inhibitor romidepsin has differential activity in core binding factor acute myeloid leukemia". ... AML1 or RUNX1 is a DNA-binding transcription factor located at the 21q22. ETO is a protein with transcriptional repressing ...
The protein encoded by this gene is an RNA binding protein and possible splicing factor. The encoded protein is found in the ... nucleus, where it colocalizes with core spliceosomal proteins. Studies of a mouse protein with high sequence similarity to this ... "Entrez Gene: RBM39 RNA binding motif protein 39". CS1 maint: discouraged parameter (link) Jung DJ, Na SY, Na DS, Lee JW (Jan ... RNA-binding protein 39 is a protein that in humans is encoded by the RBM39 gene. ...
The core recommendations of this group took account of all psychological theory and spiritual factors in the human condition. " ... Service users who feel rejected are bound to escalate their behaviour in order to be heard and in order to "hit back" at those ... "Psychological mindedness" was the concept and the phrase that seemed to bind everything together. This work has led to the ... Conte HR, Ratto R, Karusa T (1996). "The Psychological Mindedness Scale: Factor structure and relationship to outcome of ...
The degree to which the ETS1 transcription factor can bind to its binding sites on the PARP1 promoter depends on the ... helix DNA binding motif known as the Ets domain that specifically recognizes DNA sequences that contain a GGAA/T core element. ... Bi FF, Li D, Yang Q (2013). "Hypomethylation of ETS transcription factor binding sites and upregulation of PARP1 expression in ... The later suggests that several other transcription factors may facilitate Ets1 binding to unfavorable DNA sequences. ChIP-Seq ...
Binding to HS stabilizes fibroblast growth factors (FGFs) and vascular endothelial growth factors (VEGFs) and prevents them ... The basic HSPG structure consists of a protein core to which several linear heparin sulfate (HS) chains are covalently O-linked ... Growth factors such as epidermal growth factor (EGF), fibroblast growth factor (FGF) and transforming growth factor beta (TGF-β ... heparin-binding growth factors, chemokines and lipoproteins. HSPGs are prominent components of blood vessels. ...
"New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ... The process is more complicated, and at least seven different factors are necessary for the binding of an RNA polymerase II to ...
The proximal promoter region binds transcription factors that modify the affinity of the core promoter for RNA polymerase. ... For example, some repressor proteins can bind to the core promoter to prevent polymerase binding. For genes with multiple ... The core promoter marks the start site for transcription by binding RNA polymerase and other proteins necessary for copying DNA ... The binding of different transcription factors, therefore, regulates the rate of transcription initiation at different times ...
Yki is unable to bind DNA by itself. In its active state, Yki binds to the transcription factor Scalloped (Sd), and the Yki-Sd ... The Hippo pathway consists of a core kinase cascade in which Hpo phosphorylates (Drosophila) the protein kinase Warts (Wts). ... YAP and TAZ can bind to several transcription factors including p73, Runx2 and several TEADs. YAP regulates the expression of ... Hpo can bind to and phosphorylate Sav, which may function as a scaffold protein because this Hpo-Sav interaction promotes ...
Proneural proteins specifically bind DNA sequences that contain a core hexanucleotide motif, CANNTG, known as an E-box. The ... Because heterodimerization is a prerequisite for DNA binding, factors that interfere with dimerization effectively act as ... they express a membrane bound ligand, called ''Delta'', which binds and activate Notch receptors expressed in neighboring cells ... contacts between bHLH residues and DNA are responsible for the common ability of neural bHLH proteins to bind to the core E- ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ... Binding and activation[edit]. Ligand binding is an equilibrium process. Ligands bind to receptors and dissociate from them ... Allosteric modulators: They do not bind to the agonist-binding site of the receptor but instead on specific allosteric binding ...
These core binding factors, or nuclear factors (NF-Y), are composed of three subunits - NF-YA, NF-YB, and NF-YC. Whereas in ... It is essential to the transcription that these core binding factors (also referred to as nuclear factor Y or NF-Y) are able to ... the core binding factor (CBF)-DNA complex retains a high degree of conservation within the CCAAT binding motif, as well as the ... In an experiment done with core binding factors (CBF) and DNA complexes, researchers were able to determine the preferential ...
"Competitive binding assay of src homology domain 3 interactions between 5-lipoxygenase and growth factor receptor binding ... A novel Sp1-binding site occurs close to the major transcription start site (position - 65); a GC-rich core region including ... ALOX5 binds with the F actin-binding protein, coactin-like protein. Based on in vitro studies, this protein binding serves to ... The upstream promoter in the human ALOX5 gene commonly possess five GGGCCGG repeats which bind the Sp1 transcription factor and ...
This model was said to be found in EU core countries such as Belgium, France, Germany, the Netherlands and Italy, and it is ... Trade unionism in the United Kingdom was a major factor in some of the economic crises during the 1960s and the 1970s, ... The agreements negotiated by a union are binding on the rank and file members and the employer and in some cases on other non- ... Ireland and the UK belong to this category, and in contrast to the EU core countries above, these countries first joined the EU ...
This upper bound given by Shannon's work set up a long journey in designing ECCs that can go close to the ultimate performance ... OpenAir: implementation (in C) of the 3GPP specifications concerning the Evolved Packet Core Networks. ... rectangular (or uniform) interleavers (similar to the method using skip factors described above) ... The iterative decoding algorithm works best when there are not short cycles in the factor graph that represents the decoder; ...
"Pathogenic Properties (Virulence Factors) of Some Common Pathogens" (PDF).. *^ "Clindamycin" (PDF). Davis. 2017. Retrieved ... It has a core set of 1553 genes, plus 154 genes in its virulome, which contribute to virulence and 176 genes that maintain a ... For a bacterium to bind, take up, and recombine exogenous DNA into its chromosome, it must enter a special physiological state ... They have a polysaccharide capsule that acts as a virulence factor for the organism; more than 90 different serotypes are known ...
Despite the importance of numerous psychosocial factors, at its core, drug addiction involves a biological process: the ability ... IgE circulates around and binds to receptors of cells leading to an acute inflammatory response.[13] In this case, ... As a causal factor in pathologyEdit. Sensitization has been implied as a causal or maintaining mechanism in a wide range of ... a b Croner S (1992). "Prediction and detection of allergy development: influence of genetic and environmental factors". J. ...
Antarctica was ice-bound throughout the Pleistocene and the preceding Pliocene. The Andes were covered in the south by the ... By around 200,000 BP, Middle Paleolithic stone tool manufacturing spawned a tool making technique known as the prepared-core ... caused by other factors such as disease and overhunting by humans.[16][17] New research suggests that the extinction of the ... Interpretations range from cutting and chopping tools, to digging implements, to flaking cores, to the use in traps, and as a ...
The whole core complex weighs about 500 kilodaltons. The other two proteins, Toc64 and Toc12, are associated with the core ... The editosome recognizes and binds to cis sequence upstream of the editing site. The distance between the binding site and ... probably with the help of an unknown GDP exchange factor. A domain of Toc159 might be the exchange factor that carry out the ... 14-3-3 proteins only bind to chloroplast preproteins.[43] It is also bound by the heat shock protein Hsp70 that keeps the ...
C. acnes' ability to bind and activate a class of immune system receptors known as toll-like receptors (TLRs), especially TLR2 ... High levels of growth hormone (GH) and insulin-like growth factor 1 (IGF-1) are also associated with worsened acne.[42] Both ... and TLR4, is a core mechanism of acne-related skin inflammation.[45][64][65] Activation of TLR2 and TLR4 by C. acnes leads to ... These gene candidates include certain variations in tumor necrosis factor-alpha (TNF-alpha), IL-1 alpha, and CYP1A1 genes, ...
... a k-factor is a factor that is k-regular. In particular, a 1-factor is the same thing as a perfect matching. A factor-critical ... 2. A core is a graph G such that every graph homomorphism from G to itself is an isomorphism.. 3. The core of a graph G is a ... A Moore graph is a regular graph for which the Moore bound is met exactly. The Moore bound is an inequality relating the degree ... core. 1. A k-core is the induced subgraph formed by removing all vertices of degree less than k, and all vertices whose degree ...
Another factor impacting on the construction of the airport was insolvency of general planner Planungsgemeinschaft Flughafen ... Airport head Mühlenfeld thus publicly demanded that the parties come to grips with their decision.[259] The core of the problem ... Interested citizens forced a non-binding public quorum that was held parallel to the federal election, asking whether Tegel ... 900 million in overhead costs previously mentioned are factored in. An economical estimate determined the costs for the ...
RNA polymerase II core promoter sequence-specific DNA binding. • RNA polymerase II transcription factor activity, sequence- ... DNA binding. • sequence-specific DNA binding. • transcription factor activity, sequence-specific DNA binding. • transcriptional ... RNA polymerase II core promoter proximal region sequence-specific binding. • RNA polymerase II core promoter proximal region ... "Id helix-loop-helix proteins antagonize pax transcription factor activity by inhibiting DNA binding". Molecular and Cellular ...
Gottschlich, Michele M. (2001). The Science and Practice of Nutrition Support: A Case-based Core Curriculum. Kendall Hunt. p. ... The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... Sodium tetraphenylborate can also be classified as an organosodium compound since in the solid state sodium is bound to the ... Therefore, the only factor which affects the atomic radius of the alkali metals is the number of electron shells. Since this ...
protein binding. • enzyme binding. • receptor binding. • lipid binding. • RNA polymerase II transcription factor activity, ... RNA polymerase II core promoter proximal region sequence-specific binding. • metal ion binding. • RNA polymerase II core ... DNA binding. • sequence-specific DNA binding. • transcription factor activity, sequence-specific DNA binding. • ATPase binding ... identical protein binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. • transcriptional ...
However, FEE analyses do not consider shortage the main factor,[321] while FAD-oriented scholars such as Bowbrick (1986) hold ... The general disruption of many core elements of society brought a catastrophic breakdown of sanitary conditions and hygiene ... informal creditor bound the debtor almost inescapably to the creditor/landlord; it became nearly impossible to settle the debt ... All of these factors are closely associated with the increased spread of infectious disease.[205] ...
... does not bind well to certain chemicals, including alcohols, diols, strong acids and bases, metals and most ... Since this activated carbon has no effect on blood clotting factors, heparin or most other anticoagulants [11] this allows a ... As a result, it is common for smaller waste treatment sites to ship their activated carbon cores to specialised facilities for ... Diffusion of the adsorbate is thus an important factor. These carbons are suitable for adsorption of gases and vapors, because ...
Causal determinism is the concept that events within a given paradigm are bound by causality in such a way that any state (of ... One contribution is randomness.[198] While it is established that that randomness is not the only factor in the perception of ... the hard problem of free will represents the core problem of conscious free will: Does conscious volition impact the material ... The problem of predeterminism is one that involves the factors of heredity and environment, and the point to be debated here is ...
However, other methods can be used to override this binding of a document to a form note. The resulting loose binding of ... These two factors have resulted in the user interface containing some differences from applications that only run on Windows. ... Later that year, the Domino web server was integrated into release 4.5 of the core Notes server and the entire server program ... An item named "Form" in a document note can be used to bind a document to a form design note, which directs the IBM Notes ...
The core wall lies beneath the cortex and surrounds the protoplast or core of the endospore. The core contains the spore ... These proteins tightly bind and condense the DNA, and are in part responsible for resistance to UV light and DNA-damaging ... The heat resistance of endospores is due to a variety of factors: *Calcium dipicolinate, abundant within the endospore, may ... The cortex contains an inner membrane known as the core. The inner membrane that surrounds this core leads to the endospore's ...
negative regulation of core promoter binding. • negative regulation of low-density lipoprotein receptor activity. • positive ... regulation of epidermal growth factor-activated receptor activity. • regulation of resting membrane potential. • regulation of ... PDZ domain binding. • cadherin binding. • peptidase activity. • beta-catenin binding. • protein binding. • calcium channel ... regulation of protein binding. • T cell receptor signaling pathway. • segmentation. • positive regulation of receptor recycling ...
chromatin binding. • DNA polymerase binding. • protein C-terminus binding. • protein binding. • four-way junction DNA binding. ... Sigurdsson S, Van Komen S, Petukhova G, Sung P (Nov 2002). "Homologous DNA pairing by human recombination factors Rad51 and ... ATP binding. • single-stranded DNA binding. • double-stranded DNA binding. • single-stranded DNA-dependent ATPase activity. ... nucleotide binding. • DNA binding. • DNA-dependent ATPase activity. • recombinase activity. • ...
A subsequent investigation determined that Smith's death was caused by a combination of factors, including the failure of the ... Core diving skills. *Advanced Open Water Diver. *Autonomous diver. *CMAS* scuba diver ...
... directing the binding of the RNA polymerase to a gene's promoter.[144] However, other archaeal transcription factors are closer ... The proteins that archaea, bacteria and eukaryotes share form a common core of cell function, relating mostly to transcription ... No membrane-bound organelles (questioned[56]) or nucleus. No membrane-bound organelles or nucleus. Membrane-bound organelles ... These microbes (archaea; singular archaeon) are prokaryotes, meaning they have no cell nucleus or any other membrane-bound ...
It binds transcription factors (CAAT TF or CTFs) and thereby stabilizes the nearby preinitiation complex for easier binding of ... The type of core promoter affects the level of transcription and expression of a gene. TATA-binding protein (TBP) can be ... The TATA box is the binding site of the TATA-binding protein (TBP) and other transcription factors in some eukaryotic genes. ... binds to the TATA box at its TATA-binding protein (TBP) subunit.[3] TBP binds to the minor groove[15] of the TATA box via a ...
The serotonylation potentiates the binding of the general transcription factor TFIID to the TATA box.[49] ... Core histone H2A/H2B/H3/H4. PDB rendering of Complex between nucleosome core particle (h3,h4,h2a,h2b) and 146 bp long DNA ... Histone gene transcription is controlled by multiple gene regulatory proteins such as transcription factors which bind to ... and is likely bound by other proteins to exert a repressive function. Another polycomb complex, PRC1, can bind H3K27me3[69] and ...
Thorne, Melvin J. American Conservative Thought since World War II: The Core Ideas (1990) online edition ... The "law and order" issue was a major factor weakening liberalism in the 1960s.[93] From 2001-2008, Republican President George ... We believe that truth is neither arrived at nor illuminated by monitoring election results, binding though these are for other ... They hold that military institutions embody core values such as honor, duty, courage, loyalty, and a willingness on the part of ...
The cleaved SREBP then migrates to the nucleus and acts as a transcription factor to bind to the SRE (sterol regulatory element ... of the lipoprotein particle while neutral lipids including triglycerides and cholesterol esters are carried in the core of the ... regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor". Cell 89: 331. doi:10.1016/S0092- ... Sterol Regulatory Element Binding Protein 1 and 2).[10] In the presence of cholesterol, SREBP is bound to two other proteins: ...
The phrase "core of our being" is Freudian; see Bettina Bock von Wülfingen (2013). "Freud's 'Core of our Being' Between ... Kropotkin, Peter, Mutual Aid: A Factor of Evolution (1902). *. Oord, Thomas (2004). Science of Love. Philadelphia: Templeton ... he concluded that though we are not bound to love others more than ourselves, we naturally seek the common good, the good of ... These qualities are the ultimate source of human happiness, and the need for them lies at the very core of our being" (Dalai ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ... Binding and activationEdit. Ligand binding is an equilibrium process. Ligands bind to receptors and dissociate from them ... Allosteric modulators: They do not bind to the agonist-binding site of the receptor but instead on specific allosteric binding ...
... its dependence on many crucial factors, of which natural selection is only one). ... Dynamic core hypothesis. *Damasio's theory of consciousness. *Electromagnetic theories of consciousness. *Global workspace ...
Bound by tradition. Retrieved on July 25, 2011. *^ Keng, Huay Pu. "Long Neck Ring Wearing". www.huaypukeng.com. Retrieved ... Femininity is socially constructed, but made up of both socially-defined and biologically-created factors.[1][2][3] This makes ... Friedan thinks that "the core of the problem for women today is not sexual but a problem of identity - a stunting or evasion of ... "The Art of Social Change: Campaigns against foot-binding and genital mutilation". The New York Times. October 22, 2010.. ...
protein kinase binding. • core promoter binding. • RNA polymerase II transcription coactivator activity. • transcription factor ... activating transcription factor binding. • rRNA binding. • protein N-terminus binding. • chromatin binding. ... histone binding. • Tat protein binding. • NF-kappaB binding. • ligação a proteínas plasmáticas. • RNA binding. • protein ... ribosomal large subunit binding. • protein homodimerization activity. • ribosomal small subunit binding. • unfolded protein ...
Core binding factor acute myeloid leukemia (CBF-AML) is one form of a cancer of the blood-forming tissue (bone marrow) called ... medlineplus.gov/genetics/condition/core-binding-factor-acute-myeloid-leukemia/ Core binding factor acute myeloid leukemia. ... Core binding factor acute myeloid leukemia (CBF-AML) is one form of a cancer of the blood-forming tissue (bone marrow) called ... Core-binding factor acute myeloid leukemia. Arch Pathol Lab Med. 2011 Nov;135(11):1504-9. doi: 10.5858/arpa.2010-0482-RS. ...
CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding ... "Core Binding Factors" by people in Profiles.. * Comparison of therapy-related and de novo core binding factor acute myeloid ... "Core Binding Factors" by people in Harvard Catalyst Profiles by year, and whether "Core Binding Factors" was a major or minor ... Core-binding factor acute myeloid leukemia with t(8;21): Risk factors and a novel scoring system (I-CBFit). Cancer Med. 2018 09 ...
The Core binding factor (CBF) is a group of heterodimeric transcription factors. Core binding factors are composed of: a non- ... DNA-binding CBFβ chain (CBFB) a DNA-binding CBFα chain (RUNX1, RUNX2, RUNX3) de Bruijn M, Speck N (2004). "Core-binding factors ... Core+Binding+Factors at the US National Library of Medicine Medical Subject Headings (MeSH) AI-10-49 v t e. ...
OUTCOME OF CORE BINDING FACTOR ACUTE MYELOID LEUKEMIA IN ADULT PATIENTS. PB1727. El Gammal, M.1; Mahmoud, A.2; Samra, M.1; ... Home , June 2019 - Volume 3 - Issue , OUTCOME OF CORE BINDING FACTOR ACUTE MYELOID LEUKEMIA IN ADU... ... Core binding factor acute myeloid leukemia (CBF AML) encodes two recurrent cytogentic abnormalities, t(8;21) and inv (16) and ... OUTCOME OF CORE BINDING FACTOR ACUTE MYELOID LEUKEMIA IN ADULT PATIENTS: PB1727 ...
Eukaryotic transcriptional regulation is complex. Typically, it takes place by cooperative regulation. A number of studies have characterized this aspect i
Not all patients with core binding factor acute myeloid leukemia (CBF-AML) display a good outcome. Modern risk factors include ... Prospective evaluation of gene mutations and minimal residual disease in patients with core binding factor acute myeloid ... but MRD remained the sole prognostic factor in multivariate analysis. At 36 months, cumulative incidence of relapse and RFS ...
Expression of core-binding factor a1 and osteocalcin in fluoride-treated fibroblasts and osteoblasts. Author: Duan X, Xu H, ... which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in ...
Dasatinib (Sprycel™) in Patients With Newly Diagnosed Core Binding Factor (CBF) Acute Myeloid Leukemia (AML). The safety and ... Core binding factor (CBF) AML with molecular diagnosis of RUNX1-RUNX1T1 fusion transcript resulting from t(8;21)(q22;q22) (or a ... Genetics Home Reference related topics: Core binding factor acute myeloid leukemia Cytogenetically normal acute myeloid ... Adding dasatinib to intensive treatment in core-binding factor acute myeloid leukemia-results of the AMLSG 11-08 trial. ...
Modern risk factors include KIT and/or FLT3 gene mutations and minimal residual disease (MRD) levels, but their respective ... Not all patients with core binding factor acute myeloid leukemia (CBF-AML) display a good outcome. ... Not all patients with core binding factor acute myeloid leukemia (CBF-AML) display a good outcome. Modern risk factors include ... Prospective evaluation of gene mutations and minimal residual disease in patients with core binding factor acute myeloid ...
2896 Mutational Landscape of Relapsed Core-Binding Factor Acute Myeloid Leukemia (CBF-AML) Program: Oral and Poster Abstracts. ... Background: Acute myeloid leukemias (AML) with rearrangements of core-binding factor (CBF) complex genes (CBF-AML), comprising ...
Researchers Reveal Genomic Landscape of Core-Binding Factor Acute Myeloid Leukemia. By The ASCO Post. Posted: 11/4/2016 11:21: ... These genes encode for proteins that are part of the core-binding factor (CBF) complex, a transcriptional complex essential for ... University Pediatric Cancer Genome Project has completed a detailed map of the genomic landscape for core-binding factor acute ... the Pediatric Cancer Genome Project identified several mutations that may contribute to the development of core-binding factor ...
Minimal residual disease monitoring by quantitative RT-PCR in core binding factor AML allows risk stratification and predicts ... Anthracycline dose intensification improves molecular response and outcome of patients treated for core binding factor acute ... Anthracycline dose intensification improves molecular response and outcome of patients treated for core binding factor acute ... namely core binding factor AML (CBF-AML). In recent years, several studies have pointed out the importance of minimal residual ...
Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor.. S Wang ... 4:233-242, 1990). We previously reported the purification of core-binding factors (CBF) from calf thymus nuclei (S. Wang and N. ... Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. ... Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. ...
What is core-binding factor, runt domain, alpha subunit 1? Meaning of core-binding factor, runt domain, alpha subunit 1 medical ... What does core-binding factor, runt domain, alpha subunit 1 mean? ... core-binding factor, runt domain, alpha subunit 1 explanation ... Looking for online definition of core-binding factor, runt domain, alpha subunit 1 in the Medical Dictionary? ... Core-binding factor, runt domain, alpha subunit 1 , definition of core-binding factor, runt domain, alpha subunit 1 by Medical ...
Exon 8 splice site mutations in the gene encoding the E3-ligase CBL are associated with core binding factor acute myeloid ... CBL Exon 8/9 Mutants Activate the FLT3 Pathway and Cluster in Core Binding Factor/11q Deletion Acute Myeloid Leukemia/ ... All AML/MDS patients with identified CBL mutants belonged to the core binding factor and 11q deletion AML subtypes. ... Among 43 cases of core binding factor leukemias [t(8;21) and inv(16)] two patients expressing aberrant CBL transcripts were ...
Old and new prognostic factors in acute myeloid leukemia with deranged core-binding factor beta. Am J Hematol. 2013;88(7):594- ... Prognostic factors and outcome of core binding factor acute myeloid leukemia patients with t(8;21) differ from those of ... Identification of molecular and cytogenetic risk factors for unfavorable core-binding factor-positive adult AML with post- ... Core-binding factor acute myeloid leukemia: heterogeneity, monitoring and therapy. Am J Hematol. 2014;89(12):1121-1131. ...
... and the binding of a purified core binding factor, the proteins that bound to the TCR delta core site were indistinguishable ... Indistinguishable nuclear factor binding to functional core sites of the T-cell receptor delta and murine leukemia virus ... Indistinguishable nuclear factor binding to functional core sites of the T-cell receptor delta and murine leukemia virus ... Indistinguishable nuclear factor binding to functional core sites of the T-cell receptor delta and murine leukemia virus ...
Core-binding factor β and Runx transcription factors promote adaptive natural killer cell responses ... Core-binding factor β and Runx transcription factors promote adaptive natural killer cell responses ... Core-binding factor β and Runx transcription factors promote adaptive natural killer cell responses ... Core-binding factor β and Runx transcription factors promote adaptive natural killer cell responses ...
Rna Polymerase Ii Core Promoter Proximal Region Sequence-Specific Dna Binding Transcription Factor Activity Involved In ... Rna Polymerase Ii Core Promoter Proximal Region Sequence-Specific Dna Binding Transcription Factor Activity Involved In ... Rna Polymerase Ii Core Promoter Proximal Region Sequence-Specific Dna Binding Transcription Factor Activity Involved In ... RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in ...
... binding site for THAP11 that does often overlap with SBS (STAF Binding Site) a second core-promoter associated TF binding ... Further analysis reveals that M4 is a bipartite composite cis-element, which is recognized by THAP11 via binding to the ACTAYR ... Our data strongly suggest that THAP11 and Ikaros interact directly, while NFKB1 (NF-kappa B p50) and HCF-1 are binding ... Here, we present a quantitative proteomic investigation of the M4 motif TF binding landscape in lymphoid cell lines that is ...
A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes ... Baumann, D. G., & Gilmour, D. S. (2017). A sequence-specific core promoter-binding transcription factor recruits TRF2 to ... Baumann, Douglas G. ; Gilmour, David S. / A sequence-specific core promoter-binding transcription factor recruits TRF2 to ... A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes ...
Distinct roles for c-Myb and core binding factor/polyoma enhancer-binding protein 2 in the assembly and function of a ... Edelta function depends critically on transcription factors core binding factor (CBF)/polyoma enhancer-binding protein 2 (PEBP2 ... Distinct roles for c-Myb and core binding factor/polyoma enhancer-binding protein 2 in the assembly and function of a ... In contrast, c-Myb has no apparent affect on CBF/PEBP2 binding, but is critical for transcriptional activation. Thus, our data ...
Core binding factor ? (CBF?) is retained in the midbody during cytokinesis.. 24522927. 2014. CBF? enhances de novo protein ... Core-binding factor subunit beta is not required for non-primate lentiviral Vif-mediated APOBEC3 degradation.. ... The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the ... produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion ...
Port binding, part of Cloud Native Twelve-Factor Applications ... P Moley III for an in-depth discussion in this video Factor 7: ... Practical Application Architecture with Entity Framework Core. with Richard Goforth. 2h 42m Advanced ... Join Frank P Moley III for an in-depth discussion in this video Factor 7: Port binding, part of Cloud Native Twelve-Factor ... the 12-factor patterns use a strategy of port binding.… Each process has its communication protocols bound…to a usually non- ...
Core binding factor acute myeloid leukemia. MedlinePlus Genetics provides information about Core binding factor acute myeloid ... KIT gene mutations associated with GISTs create a protein that no longer requires binding of the stem cell factor protein to be ... binds) to it. This binding turns on (activates) the KIT protein, which then activates other proteins inside the cell by adding ... The KIT protein is found in the cell membrane of certain cell types where a specific protein, called stem cell factor, attaches ...
Translokacje chromosomalne modyfikujące Core-Binding Factor[edytuj , edytuj kod]. Ostra białaczka mielobastyczna z cechami ... Core-binding factor acute myeloid leukemia.. „Arch Pathol Lab Med". 135 (11), s. 1504-9, Nov 2011. DOI: 10.5858/arpa.2010-0482- ... Core binding factor genes and human leukemia. „Haematologica". 87 (12), s. 1307-23, Dec 2002. PMID: 12495904. ... Core-binding factor acute myeloid leukemia: can we improve on HiDAC consolidation?. „Hematology Am Soc Hematol Educ Program". ...
However, some transcription factors are known to exhibit some flexibility and bind to DNA in more than one possible physical ... Variable structure motifs for transcription factor binding sites By J. E. (John E.) Reid, Kenneth J. Evans, Nigel Dyer, Lorenz ... 2008). Sidow A: Genome-wide analysis of transcription factor binding sites based on ChIP-Seq data. Nat Methods ... 2003). Wingender E: MATCH: A tool for searching transcription factor binding sites in DNA sequences. Nucleic Acids Res ...
Core Binding Factor Leukemia: Chromatin Remodeling Moves Towards Oncogenic Transcription *Alessandro Beghini ... Non-DNA-binding Ikaros isoforms gene expressed in adult B-precursor acute lymphoblastic leukemia. Leukemia 2002; 16: 1285-1292. ... mouse monoclonal antibody against human granulocyte/macrophage colony-stimulating factor (GM-CSF) receptor α chain (GM-CSFRα) ... and granulocyte colony-stimulating factor (G-CSF) receptor (G-CSFR) were purchased from PharMingen (San Diego, CA, USA). Mouse ...
Core. Binding. Factor. Alpha. 2. Subunit. /*genetics Core. Binding. Factor. Alpha. 3 Subunit. /*genetics Precursor Cells, T- ... Core. Binding. Factor. Alpha. 2. Subunit. /deficiency ; Core. Binding. Factor. Alpha. 2. Subunit. /genetics ; Gene Knock-In ... Core. Binding. Factor. Alpha. 2. Subunit. /immunology ; Core. Binding. Factor. Alpha. 3 Subunit. /immunology ; Female ; Gene ... Core. Binding. Factor. Alpha. 2. Subunit. /*genetics DNA-. Binding. Proteins/*genetics Guanine Nucleotide Exchange Factors/* ...
Comprehensive mutational profiling of core binding factor acute myeloid leukemia.. Duployez N1, Marceau-Renaut A1, Boissel N2, ... have been recognized as unique entities within AML and are usually reported together as core binding factor AML (CBF-AML). ... in the Phase 3 Trial of Systematic Versus Response-adapted Timed-Sequential Induction in Patients With Core Binding Factor ...
  • These genes encode for proteins that are part of the core-binding factor (CBF) complex, a transcriptional complex essential for normal blood cell development. (ascopost.com)
  • For example, the fusion proteins AML1-ETO and core binding factor β (CBFβ)-MYH11 resulting from the t(8;21)(q22;q22) and (inv 16)(p13;q22) or t(16;16)(p13;q22), respectively, have been shown to act as transcriptional repressors. (aacrjournals.org)
  • On the basis of electrophoretic mobility shift binding and competition studies, biochemical analysis of affinity-labeled DNA-binding proteins, and the binding of a purified core binding factor, the proteins that bound to the TCR delta core site were indistinguishable from those that bound to the murine leukemia virus core site. (asm.org)
  • These data argue that DNA-binding proteins that interact with the core site of murine leukemia virus long terminal repeats and contribute to viral T-cell tropism also play an essential role in the T-cell-specific expression of cellular genes. (asm.org)
  • 2001). DNA binding specificity of different STAT proteins. (core.ac.uk)
  • For instance, the Dehydration-Responsive Element Binding Proteins (DREB1A and DREB2A) bind to the DRE box (5′-[A/G]CCGAC-3′) of dehydration- and cold stress-related genes (Liu et al. (springer.com)
  • CBFβ attaches (binds) to one of three related RUNX proteins (RUNX1, RUNX2, or RUNX3) to form different versions of CBF. (nih.gov)
  • Insulin-like growth factors (IGFs) are bound by several IGF-binding proteins (IGFBPs) that appear to regulate IGF transportation, receptor binding and action. (core.ac.uk)
  • Transcription factors PEA1/AP1, PEA2/PEBP2, and PEA3/ETS were identified as A element-binding proteins. (nih.gov)
  • Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. (wikipedia.org)
  • Together the data indicate that TGF-β1 induction of the α promoter involves induction of CBFα3, which binds to the TβRE of the promoter along with one or more proteins. (jimmunol.org)
  • Proteins belonging to the MADS family function as dimers, the primary DNA-binding element of which is an anti-parallel coiled coil of two amphipathic alpha-helices, one from each subunit. (ebi.ac.uk)
  • The binding of transcription factor proteins (TFs) to DNA promoter regions upstream of gene transcription start sites (TSSs) is one of the most important mechanisms by which gene expression, and thus many cellular processes, are controlled. (bioconductor.org)
  • The main components include IGF-I, IGF-2, the two IGF receptors (IGF-1R and IGF-2R), the six binding proteins (IGFBP1-6), the acid-labile subunit, and the upstream and downstream regulatory factors. (aacrjournals.org)
  • Some of the proteins produced by the epididymis, called sperm decapacitating factors, may associate to the cell surface and prevent premature sperm activation. (scielo.cl)
  • Other epididymal proteins may act directly in the process of gamete recognition and interaction, by remaining on spermatozoa to mediate sperm binding to the zona pellucida and oocyte plasma membrane. (scielo.cl)
  • NAC proteins constitute one of the largest families of plant-specific transcription factors, and a number of these proteins participate in the regulation of plant development and responses to abiotic stress. (plantphysiol.org)
  • Using the yeast one-hybrid system, we isolated three cDNA clones encoding proteins that bind to the 63-bp promoter region of erd1 , which contains the CATGTG motif. (plantcell.org)
  • These three cDNA clones encode proteins named ANAC019, ANAC055, and ANAC072, which belong to the NAC transcription factor family. (plantcell.org)
  • The envelope (E), membrane (M), and spike (S) proteins localized at vial surface suggested having host binding capacity. (frontiersin.org)
  • In general at the initial phase of the host binding, virus may create an optimal environment for its replication by interacting with the host proteins. (frontiersin.org)
  • 4 ] 5mC is recognized by methyl DNA binding proteins through specific peptide modules such as MBD (methyl-CpG binding domain) or triple-zinc-finger motif. (wiley.com)
  • Substances known as transcription factors often determine how a cell develops as well as which proteins it produces and in what quantities. (mpg.de)
  • Until recently, scientists had suspected that certain proteins only bind to specific sites on the DNA strand, directing the cell's fate in the process. (mpg.de)
  • These proteins, known as transcription factors, control the activity of genes. (mpg.de)
  • At these events, the binding of ribosomal proteins stabilizes the RNA folding process and eventually drives the RNA structure to the final native state. (wikibooks.org)
  • [7] As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. (wikiversity.org)
  • Two of these genes, RUNX1 and CBFB , provide instructions for making the two pieces of a protein complex known as core binding factor (CBF). (medlineplus.gov)
  • The fusion protein can form CBF, but it is thought that the presence of the MYH11 portion of the fusion protein prevents CBF from binding to DNA, impairing its ability to control gene activity. (medlineplus.gov)
  • A common chromosomal translocation in acute myeloid leukemia (AML) involves the AML1 (acute myeloid leukemia 1, also called RUNX1, core binding factor protein (CBF alpha), and PEBP2 alpha B) gene on chromosome 21 and the ETO (eight-twenty one, also called MTG8) gene on chromosome 8. (curehunter.com)
  • This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. (ihcworld.com)
  • However, CBF beta p22.0 and CBF beta p21.5 form a complex with DNA-binding CBF alpha subunits and as a result decrease the rate of dissociation of the CBF protein-DNA complex. (asm.org)
  • A gene on chromosome 6p21 that encodes a nuclear protein member of the RUNX family of transcription factors with a Runt DNA-binding domain. (thefreedictionary.com)
  • Expression of CBLΔexon8 and CBLΔexon8+9 in FLT3-WT-Ba/F3 cells induced growth factor-independent proliferation associated with autophosphorylation of FLT3 and activated the downstream targets signal transducer and activator of transcription 5 (STAT5) and protein kinase B (AKT). (aacrjournals.org)
  • In this study, we show that protein binding to the core motif is necessary but not sufficient for transcriptional activation by the delta E3 element. (asm.org)
  • In contrast, protein binding to the E-box motif does not contribute significantly to enhancer activity. (asm.org)
  • In the absence of Ikaros, protein levels of NFKB1 (NF-kappa B p50) were virtually not affected (lane 4 vs 1) but its binding to the M4 column was completely abrogated (lane 5). (biomedcentral.com)
  • Recently, TATA-binding protein-related factor 2 (TRF2) rather than the TATA-binding protein (TBP) was found to function in transcription of RP genes in Drosophila. (elsevier.com)
  • Baumann, DG & Gilmour, DS 2017, ' A sequence-specific core promoter-binding transcription factor recruits TRF2 to coordinately transcribe ribosomal protein genes ', Nucleic acids research , vol. 45, no. 18, pp. 10481-10491. (elsevier.com)
  • Distinct roles for c-Myb and core binding factor/polyoma enhancer-binding protein 2 in the assembly and function of a multiprotein complex on the TCR delta enhancer in vivo. (duke.edu)
  • Edelta function depends critically on transcription factors core binding factor (CBF)/polyoma enhancer-binding protein 2 (PEBP2) and c-Myb as measured by transcriptional activation of transiently transfected substrates in Jurkat cells, and by activation of V(D)J recombination within chromatin-integrated substrates in transgenic mice. (duke.edu)
  • To understand the molecular mechanisms for synergy between these transcription factors in the context of chromatin, we used in vivo footprinting to study the requirements for protein binding to Edelta within wild-type and mutant versions of a human TCR delta minilocus in stably transfected Jurkat cells. (duke.edu)
  • The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g. (nih.gov)
  • 2002). An algorithm for finding protein-DNA binding sites with applications to chromatin-immunoprecipitation microarray experiments. (core.ac.uk)
  • 2007). Analysis of the vertebrate insulator protein CTCF-binding sites in the human genome. (core.ac.uk)
  • 2009). Bulyk ML: UniPROBE: an online database of protein binding microarray data on protein-DNA interactions. (core.ac.uk)
  • 2009). Bulyk ML: Universal protein-binding microarrays for the comprehensive characterization of the DNA-binding specificities of transcription factors. (core.ac.uk)
  • Ethylene-responsive element binding protein, a member of the APETALA2/ethylene response factor (AP2/ERF) superfamily, is a transcription factor that regulates stress-responsive genes by recognizing a specific cis -acting element of target DNA. (springer.com)
  • 1994 ). Additionally, an AP2-LIKE ERE BINDING FACTOR (ERF), also known as ERE BINDING PROTEIN (EREBP), was first isolated as the GCC box-binding protein from tobacco (Ohme-Takagi and Shinshi 1995 ). (springer.com)
  • CBFB (Core-Binding Factor Beta Subunit) is a Protein Coding gene. (genecards.org)
  • The CBFB gene provides instructions for making a protein called core binding factor beta (CBFβ), which is one piece of a protein complex known as core binding factor (CBF). (nih.gov)
  • These protein complexes bind to specific regions of DNA and help turn on (activate) certain genes. (nih.gov)
  • The presence of CBFβ helps the complex bind to DNA and protects the RUNX protein from being broken down. (nih.gov)
  • Once bound to DNA, the RUNX1 protein controls the activity of genes involved in the development of blood cells (hematopoiesis). (nih.gov)
  • The protein produced from the fusion gene, called CBFβ-MYH11, can still bind to RUNX1 to form CBF. (nih.gov)
  • The protein produced from the FOXF1 gene is a transcription factor, which means that it attaches (binds) to specific regions of DNA and helps control the activity of many other genes. (nih.gov)
  • Here, we use it as an exemplar system to show how native top-down and bottom-up mass spectrometry can measure the structural effect of cofactor binding by a protein. (nature.com)
  • HDX-MS reveals that while the dominant structural changes occur proximal to the cofactor-binding site, rearrangements on cofactor binding are evident throughout the protein, predominantly attributable to allosteric conformational tightening, consistent with IM-MS data. (nature.com)
  • Determination of the crystal structure of the A. niger Fdc1 UbiX revealed the presence of a modified flavin mononucleotide (FMN) cofactor bound to the protein in complex with Mn 2+ and K + . This modified cofactor (prenylated FMN or prFMN) results from addition of a prenyl group to the N5-C6 atoms of FMN to form a fourth, non-aromatic ring. (nature.com)
  • Identification of insulin-like growth factor (IGF)-I and IGF-binding protein in chylous ascites. (core.ac.uk)
  • This idea is supported by direct contact in vitro between activators and GTFs, including TATA box-binding protein (TBP) ( 2 ), TFIIB ( 3 ), TFIIF ( 4 ), TFIIH ( 5 ), as well as the Rpb5 subunit of RNAPII ( 6 ), and TBP-associated factors ( 7 , 8 ). (pnas.org)
  • Yeast TFIIB is a monomeric protein of 345 aa that is structurally similar to human TFIIB, including a zinc binding motif near the N terminus and two imperfect repeats encompassing the C-terminal two-thirds of the molecule. (pnas.org)
  • This study aimed to investigate the long-term effect of fenofibrate therapy on three novel biomarkers of cardiovascular risk, namely adipocyte-fatty acid binding protein (A-FABP), fibroblast growth factor 21 (FGF21), and retinol-binding protein 4 (RBP4), which are all downstream targets of PPAR-alpha or PPAR-gamma, in patients with type 2 diabetes. (ahajournals.org)
  • The membrane-spanning anti-sigma factor is first cut extracytoplasmically (site-1 protease, S1P), then within the membrane itself (site-2 protease, S2P, Rip1), while cytoplasmic proteases finish degrading the regulatory protein, liberating SigM (Probable). (uniprot.org)
  • binding domain present within the protein. (uniprot.org)
  • section specifies the position and type of each DNA-binding domain present within the protein. (uniprot.org)
  • and RUNX1) of a protein complex known as core binding factor (CBF). (ivami.com)
  • This protein complex binds to specific regions of DNA and helps to activate specific genes. (ivami.com)
  • of the fusion protein prevents binding to DNA CBF, affecting its ability to control gene activity. (ivami.com)
  • Nuclear factor of activated T-cells, cytoplasmic 1 is a protein that in humans is encoded by the NFATC1 gene . (wikipedia.org)
  • Despite the importance of the TβRE for GL α transcription, no protein that binds the TβRE and regulates the GL α promoter has been identified. (jimmunol.org)
  • All the α subunits contain a highly conserved runt domain that is responsible for both DNA binding and dimerization with the CBFβ subunit ( 15 , 16 , 17 , 18 , 19 , 20 ), which by itself cannot bind DNA but increases the affinity of CBFα protein for DNA about fivefold ( 16 , 17 , 18 ). (jimmunol.org)
  • CBFα is also known as acute myeloid leukemia (AML) or polyomavirus enhancer core-binding protein-2α (PEBP2α). (jimmunol.org)
  • Human serum response factor (SRF) is a ubiquitous nuclear protein important for cell proliferation and differentiation. (ebi.ac.uk)
  • Circulating levels of insulin-like growth factor I (IGF-I) and its main binding protein, IGF binding protein 3 (IGFBP-3), have been associated with risk of several types of cancer. (aacrjournals.org)
  • IGF-I and its major binding protein, IGFBP3, are two of the key molecules in this pathway. (aacrjournals.org)
  • The MYC-like sequence CATGTG plays an important role in the dehydration-inducible expression of the Arabidopsis thaliana EARLY RESPONSIVE TO DEHYDRATION STRESS 1 ( ERD1 ) gene, which encodes a ClpA (ATP binding subunit of the caseinolytic ATP-dependent protease) homologous protein. (plantcell.org)
  • Most of these steps are carried simultaneously with the transcription of the ribosomal RNAs, and the ribosome assembly process is carried through protein-binding events and RNA conformational changes. (wikibooks.org)
  • The assembly of bacterial ribosomes is thought to progress through an alternating series of RNA conformational changes as well as protein-binding events. (wikibooks.org)
  • Initiation of transcription by pol I is dependent on the presence of the transcription initiation factors TATA-binding protein (TBP), core factor (CF), upstream activating factor (UAF), and Rrn3p, which have yet to be identified in a preassembled complex. (rupress.org)
  • When there is no TATA box nucleotide sequence in the gene core promoter region of the DNA next to a gene, say A1BG of the human genome, a TATA binding protein associated factor (TAF) will bind sequence specifically and force the TATA box binding protein to bind non-sequence specifically to the DNA in the core promoter . (wikiversity.org)
  • let the symbol TAF stand for TATA binding protein associated factor . (wikiversity.org)
  • any factor associating with the TATA binding protein (TBP) is called a TBP associated factor (TAF). (wikiversity.org)
  • The TATA-binding protein ( TBP ) is a general transcription factor that binds specifically to a DNA sequence called the TATA box . (wikiversity.org)
  • Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. (wikipedia.org)
  • Core binding factor acute myeloid leukemia (CBF-AML) is one form of a cancer of the blood-forming tissue (bone marrow) called acute myeloid leukemia. (medlineplus.gov)
  • Core Binding Factor (CBF) Acute Myeloid Leukemia (AML) is cytogenetically characterized by either the t(8;21) or the inv(16)/t(16;16) chromosomal abnormalities, which, although being pathognomonic, are not sufficient per se to induce overt leukemia but rather determine a preclinical phase of disease when preleukemic subclones compete until the acquisition of clonal dominance by one of them. (hindawi.com)
  • Comparison of therapy-related and de novo core binding factor acute myeloid leukemia: A bone marrow pathology group study. (harvard.edu)
  • Core-binding factor acute myeloid leukemia with t(8;21): Risk factors and a novel scoring system (I-CBFit). (harvard.edu)
  • Adding dasatinib to intensive treatment in core-binding factor acute myeloid leukemia-results of the AMLSG 11-08 trial. (harvard.edu)
  • Dasatinib in high-risk core binding factor acute myeloid leukemia in first complete remission: a French Acute Myeloid Leukemia Intergroup trial. (harvard.edu)
  • Population-based disparities in survival among patients with core-binding factor acute myeloid leukemia: a SEER database analysis. (harvard.edu)
  • Prospective evaluation of gene mutations and minimal residual disease in patients with core binding factor acute myeloid leukemia. (harvard.edu)
  • The t(8;21), associated with de novo acute myeloid leukemias, disrupts the CBFA2 ( AML1 ) gene, which encodes a DNA-binding CBF α subunit. (aacrjournals.org)
  • The CBFB gene, which encodes the non-DNA-binding subunit of the CBFs, is disrupted by the inv(16) in de novo acute myeloid leukemias. (aacrjournals.org)
  • Core-binding factor acute myeloid leukemia (CBF-AML) data in Asian countries has been rarely reported. (springer.com)
  • Not all patients with core binding factor acute myeloid leukemia (CBF-AML) display a good outcome. (nih.gov)
  • Acute myeloid leukemias (AML) with rearrangements of core-binding factor (CBF) complex genes (CBF-AML), comprising t(8;21) and inv(16) subgroups, are considered as diseases with favorable outcome. (confex.com)
  • Researchers in the Pediatric Cancer Genome Project identified several mutations that may contribute to the development of core-binding factor acute myeloid leukemia as cooperating mutations, including changes in CCND2 , and new recurrent mutations, including alterations in DHX15 . (ascopost.com)
  • An international team of researchers from the St. Jude Children's Research Hospital-Washington University Pediatric Cancer Genome Project has completed a detailed map of the genomic landscape for core-binding factor acute myeloid leukemia (CBF-AML). (ascopost.com)
  • Core binding factor acute myeloid leukemia (AML) comprises two subtypes with distinct cytogenetic abnormalities of either t(8;21)(q22;q22) or inv(16)(p13q22)/t(16;16)(p13;q22). (haematologica.org)
  • Core binding factor (CBF) leukemia represents up to 12% of all newly diagnosed adult acute myeloid leukemia (AML). (haematologica.org)
  • Comprehensive mutational profiling of core binding factor acute myeloid leukemia. (cdc.gov)
  • Acute myeloid leukemia (AML) with t(8;21) or inv(16) have been recognized as unique entities within AML and are usually reported together as core binding factor AML (CBF-AML). (cdc.gov)
  • To address these issues, we performed extensive mutational analysis by high-throughput sequencing in 215 patients with CBF-AML enrolled in the Phase 3 Trial of Systematic Versus Response-adapted Timed-Sequential Induction in Patients With Core Binding Factor Acute Myeloid Leukemia and Treating Patients with Childhood Acute Myeloid Leukemia with Interleukin-2 trials (age, 1-60 years). (cdc.gov)
  • SNP-array lesions in core binding factor acute myeloid leukemia. (cdc.gov)
  • Activating mutations in RAS and receptor tyrosine kinases such as KIT and FLT3 are hypothesized to cooperate with chimeric transcription factors in the pathogenesis of acute myeloid leukemia (AML). (elsevier.com)
  • KIT receptor tyrosine kinase mutations are implicated as a prognostic factor in adults with core binding factor (CBF) acute myeloid leukemia (AML). (elsevier.com)
  • Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. (unibo.it)
  • In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. (unibo.it)
  • Clinical outcome and mutations of 96 core-binding factor acute myeloid leukemia (AML) patients 18-60 years old were examined. (hku.hk)
  • Acute myeloid leukemia factor nuclear binding (CBF-LMA) is a form of neoplasia marrow called acute myelogenous leukemia. (ivami.com)
  • Objective To assess the prevalence of c-Kit and FLT3 gene mutations in core binding factor related acute myeloid leukemia (CBF-AML) and analyze the karyotype characteristics of the CBF-AML patients . (bvsalud.org)
  • Core binding factor acute myeloid leukemia (CBF-AML): is high-dose Ara-C (HDAC) consolidation as effective as you think? (lww.com)
  • Core binding factor acute myeloid leukaemia is a cancer related to genetic changes in the CBF gene. (wikipedia.org)
  • Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression. (harvard.edu)
  • Excellent examples are the chromosomal rearrangements t(8;21)(q22;q22), t(12;21)(p13;q22), and inv(16)(p13q22) that disrupt two of the genes encoding a small family of heterodimeric transcription factors, core-binding factors (CBFs). (aacrjournals.org)
  • Point mutations introduced into the core site significantly shifted the disease specificity of the Moloney virus from thymic leukemia to erythroid leukemia (N.A. Speck, B. Renjifo, E. Golemis, T.N. Fredrickson, J.W. Hartley, and N. Hopkins, Genes Dev. (asm.org)
  • They disrupt normal hematopoiesis dependent on core binding factor subunit α ( RUNX1 ) and β ( CBFB ) by silencing tumor suppressor genes leading to neoplastic transformation. (haematologica.org)
  • We show that the transcription factor M1BP, which associates with the core promoter region, activates transcription of RP genes. (elsevier.com)
  • The best understood of these genes encode transcription factors, such as GATA-2, RUNX1, ETV6, and C/EBPα, which establish and maintain genetic networks governing the genesis and function of blood stem and progenitor cells. (jci.org)
  • Gene expression complexity is positively correlated with the total number of bound TFs , revealing insights in the regulatory code for genes with different expression breadths. (plantcell.org)
  • Studying a single TF using ChIP (henceforth referring to both ChIP-chip and ChIP-Seq ) is already valuable to examine its DNA binding motif, identify putative target genes, and unravel its biological role through the functional analysis of its targets. (plantcell.org)
  • SRF function is essential for transcriptional regulation of numerous growth-factor-inducible genes, such as c-fos oncogene and muscle-specific actin genes. (ebi.ac.uk)
  • We avoid most of this complexity in this demonstration workflow in order to examine transcription factor binding sites in a small set of seven broadly co-expressed Saccharomyces cerevisiae genes of related function. (bioconductor.org)
  • We will see, however, that even this small collection of co-regulated genes of similar function exhibits considerable regulatory complexity, with (among other things) activators and repressors competing to bind to the same DNA promoter sequence. (bioconductor.org)
  • In S. cerevisiae, two genes have a 50% chance of having a common transcription factor binder if the correlation between their expression profiles is equal to 0.84. (bioconductor.org)
  • We presume, following Allocco, that such correlation among genes, including one transcription factor, is a plausible place to look for shared transcription factor binding sites. (bioconductor.org)
  • The target sequence of the promoter from the target genes also was identified, and TsNAC1 was shown to target the positive regulators of ion transportation, such as T. HALOPHILA H + -PPASE1 , and the transcription factors MYB HYPOCOTYL ELONGATION-RELATED and HOMEOBOX12 . (plantphysiol.org)
  • Sometimes glucocorticoid receptor binding results in strong activation of neighbouring genes, whereas at other times little if anything changes," Meijsing reports. (mpg.de)
  • Scientists had previously assumed that these segments exert an effect by inhibiting the binding of transcription factors, thus impeding the activity of neighbouring genes," Meijsing says. (mpg.de)
  • Escherichia coli NikR regulates cellular nickel uptake by binding to the nik operon in the presence of nickel and blocking transcription of genes encoding the nickel uptake transporter. (harvard.edu)
  • [3] approximately 24% of human genes contain a TATA box within the core promoter. (wikiversity.org)
  • Your search returned 139 nuclear transcription factor Y subunit beta Antibodies across 25 suppliers. (biocompare.com)
  • Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. (asm.org)
  • CBF binds to core sites in murine leukemia virus and T-cell receptor enhancers. (asm.org)
  • Indistinguishable nuclear factor binding to functional core sites of the T-cell receptor delta and murine leukemia virus enhancers. (asm.org)
  • We therefore determined the relationship between the nuclear factors that bind to the TCR delta and Moloney murine leukemia virus core motifs. (asm.org)
  • CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. (nih.gov)
  • it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. (nih.gov)
  • Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. (nih.gov)
  • The role of core binding factor in the pathogenesis of the Moloney murine leukemia virus. (dartmouth.edu)
  • Of the children with AML, 40% had a mutation in KIT (11.3%), RAS (18%) or FLT3/ITD (11.1%), and 70% of cases of core-binding factor (CBF) leukemia were associated with a mutation of KIT or RAS. (elsevier.com)
  • KDM1A plays an important role in hematopoiesis and was identified as a dependency factor in leukemia stem cell populations. (aacrjournals.org)
  • and core binding factor leukemia, a rare blood and bone marrow cancer. (nih.gov)
  • Modern risk factors include KIT and/or FLT3 gene mutations and minimal residual disease (MRD) levels, but their respective values have never been prospectively assessed. (nih.gov)
  • Higher WBC, KIT, and/or FLT3-ITD/TKD gene mutations, and a less than 3-log MRD reduction after first consolidation, were associated with a higher specific hazard of relapse, but MRD remained the sole prognostic factor in multivariate analysis. (nih.gov)
  • It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. (ihcworld.com)
  • 2002). Chu G: p53 Binds and activates the xeroderma pigmentosum DDB2 gene in humans but not mice. (core.ac.uk)
  • GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and transcription coactivator activity . (genecards.org)
  • The presence of CBFβ-MYH11 may block binding of CBF to DNA, preventing RUNX1 from controlling gene activity. (nih.gov)
  • A yeast mutant was isolated encoding a single amino acid substitution [serine-53 → proline (S53P)] in transcription factor TFIIB that impairs activation of the PHO5 gene in response to phosphate starvation. (pnas.org)
  • Transcriptional activators stimulate gene expression by binding enhancer elements and contacting, either directly or indirectly, components of the RNA polymerase II (RNAPII) transcriptional machinery. (pnas.org)
  • BACKGROUND: Transcription factors (TFs) and their binding sites (TFBSs) play a central role in the regulation of gene expression. (biomedsearch.com)
  • The two are connected by gene expression, and it is generally thought that variation in transcription factor ( TF ) function is an important determinant of phenotypic evolution. (plantcell.org)
  • Possible explanations are the dependency on other condition-specific factors, such as cofactors or chromatin remodeling, for the correct regulation of the target gene, or that many of the observed binding events are nonfunctional. (plantcell.org)
  • The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. (wikipedia.org)
  • T. HALOPHILA STRESS RELATED NAC1 ( TsNAC1 ), cloned from the halophyte Thellungiella halophila , is a NAC transcription factor gene, and its overexpression can improve abiotic stress resistance, especially in salt stress tolerance, in both T. halophila and Arabidopsis ( Arabidopsis thaliana ) and retard the growth of these plants. (plantphysiol.org)
  • Transcription factors bind to a section of DNA and control how strongly a gene in that section is activated. (mpg.de)
  • Scientists had previously assumed that gene activity is controlled by the binding strength and the proximity of the binding site to the gene. (mpg.de)
  • Neighbouring DNA segments have a significant impact on transcription factor shape, thus modulating the activity of the gene. (mpg.de)
  • The closer and more tightly they bind to a gene on the DNA, the more active the gene was thought to be. (mpg.de)
  • Consequently, transcription factors can bind to DNA segments without affecting a nearby gene. (mpg.de)
  • Other factors must also be involved in determining how strongly a transcription factor activates a gene. (mpg.de)
  • The receptors simultaneously act as transcription factors and regulate gene activity in the cells. (mpg.de)
  • The scientists found that the composition of DNA segments to which the receptors bind help determine how strongly a gene is activated. (mpg.de)
  • The structure of the interface between the transcription factor and genome segments must therefore play a key role in determining gene activity. (mpg.de)
  • Binding of transcription factors (TFs) to regulatory sequences is a pivotal step in the control of gene expression. (weizmann.ac.il)
  • Core binding factors are composed of: a non-DNA-binding CBFβ chain (CBFB) a DNA-binding CBFα chain (RUNX1, RUNX2, RUNX3) de Bruijn M, Speck N (2004). (wikipedia.org)
  • CBFB enhances DNA binding by RUNX1. (nih.gov)
  • uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a fluorescence resonance energy transfer (FRET) assay. (nih.gov)
  • Confirmation of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a time resolved fluorescence resonance energy transfer (TR-FRET) assay. (nih.gov)
  • Many of the mutations we identified interfered with molecular signaling or epigenetic factors. (ascopost.com)
  • However, we identified preferential association of CBL exon8/9 mutations with core binding factor leukemias and 11q aberrations. (aacrjournals.org)
  • Here we illustrate mechanistic advances in our understanding of familial MDS/AML syndromes caused by germline mutations of hematopoietic transcription factors. (jci.org)
  • Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. (harvard.edu)
  • Association of the CBF beta subunits does not extend the phosphate contacts in the binding site. (asm.org)
  • Purification and cDNA cloning revealed that PEBP2 has two subunits, DNA-binding alpha (PEBP2alpha) and non-DNA-binding beta (PEBP2beta). (nih.gov)
  • CBF binds to DNA as a heterodimer of the α and β subunits. (jimmunol.org)
  • Our data indicate that CBF/PEBP2 plays primarily a structural role as it induces a conformational change in the enhanceosome that is associated with augmented binding of c-Myb. (duke.edu)
  • In contrast, c-Myb has no apparent affect on CBF/PEBP2 binding, but is critical for transcriptional activation. (duke.edu)
  • Core-binding factor (CBF), which interacts with a murine retrovirus enhancer, was found to be identical to PEBP2. (nih.gov)
  • A major determinant of the thymic disease specificity of Moloney virus genetically maps to the conserved viral core motif in the Moloney virus enhancer. (asm.org)
  • A similar core motif present within the enhancers of T-cell-tropic murine retroviruses has been shown to contribute to transcriptional activity of the viral long terminal repeat in T lymphocytes and to viral T-cell tropism. (asm.org)
  • To create motif logo of transcript factor for preview. (umassmed.edu)
  • Core binding factor A1 (CBFA1/RUNX2) is a runt-like transcription factor essential for osteoblast differentiation. (ihcworld.com)
  • The Core binding factor (CBF) is a group of heterodimeric transcription factors. (wikipedia.org)
  • Rabbit polyclonal to AML1.Core binding factor CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. (labourlists.org)
  • To study the effects and importance of fluoride on FBs in the development of extraperiosteal calcification and the ossification of skeletal fluorosis, the presence of the osteogenic phenotype, which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in osteoblasts (OBs) exposed to fluoride was determined. (fluoridealert.org)
  • Core binding factor a1 (CBFA1) is a key regulator of osteoblast differentiation. (scialert.net)
  • The expressions of CBFA1 and alkaline phosphatase (ALP), osteopontin (OPN), osteoprotegrin (OPG), and receptor activator nuclear factor kappa B ligand (RANKL) were detected before and after RNA interference (RNAi) of CBFA1. (scialert.net)
  • Researchers at the Max Planck Institute for Molecular Genetics in Berlin have now discovered that the DNA segment to which a transcription factor binds can assume various spatial arrangements. (mpg.de)
  • A team of scientists headed by Sebastiaan Meijsing at the Max Planck Institute for Molecular Genetics have now come to a different conclusion: The researchers discovered that transcription factors can assume various shapes depending on which DNA segment they bind to. (mpg.de)
  • The core-binding factor (CBF) family of transcription factors plays a key role in hematopoiesis and bone ossification. (jimmunol.org)
  • We have previously shown that the delta E3 site is an essential element for transcriptional activation by the human T-cell receptor (TCR) delta enhancer and identified two factors, NF-delta E3A and NF-delta E3C, that bound to overlapping core (TGTGGTTT) and E-box motifs within delta E3. (asm.org)
  • This study describes a site-specific ChIP which aggregates the benefits of both EMSA and in vivo footprinting in only one assay, thereby allowing the individual detection and analysis of single binding motifs. (biomedsearch.com)
  • This alteration enables the specific immunoprecipitation and individual examination of occupied sites, even in a complex system of adjacent binding motifs in vivo. (biomedsearch.com)
  • The established site-specific enzyme digestion enables a reliable and individual detection option for densely arranged binding motifs in vivo not provided by e.g. (biomedsearch.com)
  • Despite many advances in the characterization of sequence motifs recognized by TFs, our ability to quantitatively predict TF binding to different regulatory sequences is still limited. (weizmann.ac.il)
  • The MADS-box family of transcription factors. (ebi.ac.uk)
  • Accordingly, activators bind general transcription factors (GTFs) to facilitate formation of the preinitiation complex (PIC). (pnas.org)
  • [6] TBP, along with a variety of TBP-associated factors , make up the TFIID , a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex . (wikiversity.org)
  • Background: Classically, models of DNA-transcription factor binding sites (TFBSs) have been based on relatively few known instances and have treated them as sites of fixed length using position weight matrices (PWMs). (core.ac.uk)
  • 2005). Assessing computational tools for the discovery of transcription factor binding sites. (core.ac.uk)
  • Site-specific chromatin immunoprecipitation: a selective method to individually analyze neighboring transcription factor binding sites in vivo. (biomedsearch.com)
  • Though in recent years many new kinds of data have become available for identifying transcription factor binding sites (TFBSs) - ChIP-seq and DNase I hypersensitivity regions among them - sequence matching continues to play an important role. (bioconductor.org)
  • Our assay thus paves the way to a more comprehensive understanding of TF binding to regulatory sequences, and allows the characterization of TF binding determinants within and outside ofcore binding sites. (weizmann.ac.il)
  • SRF-like/Type I subfamily of MADS (MCM1, Agamous, Deficiens, and SRF (serum response factor)) box family of eukaryotic transcriptional regulators [ PMID: 7744019 ]. (ebi.ac.uk)
  • Binds DNA and exists as hetero- and homo-dimers [ PMID: 11846562 , PMID: 7637780 ]. (ebi.ac.uk)
  • In SRF, the MADS box has been shown to be involved in DNA-binding and dimerisation [ PMID: 3203386 ]. (ebi.ac.uk)
  • Acetylation of the lysine tails of histones, a process mediated by histone acetyl transferases, results in loosening of DNA-histone interactions and facilitates binding of specific transcriptional coregulators, resulting in transcriptionally active chromatin. (aacrjournals.org)
  • We provide several lines of evidence that TF binding at plant HOT regions is functional, in contrast to that in animals, and not merely the result of accessible chromatin. (plantcell.org)
  • Distal bound regions are under purifying selection as well and are enriched for a chromatin state showing regulation by the Polycomb repressive complex. (plantcell.org)
  • Chromatin remodeling, methylation, histone modification, chromosome interaction, distal enhancers, and the cooperative binding of transcription co-factors all play an important role. (bioconductor.org)
  • Unlike TBP, TRF2 lacks sequence-specific DNA binding activity, so the mechanism by which TRF2 is recruited to promoters is unclear. (elsevier.com)
  • Finally, by coupling our in vitro TF binding measurements, and another application of our method probing nucleosome formation, to in vivo expression measurements carried out with the same template sequences now serving and promoters, we offer insights into mechanisms that may determine the different expression outcomes observed. (weizmann.ac.il)
  • M]ost of the diversity within metazoan core promoters appears to involve the variable occurrence of consensus or near-consensus TATA, Inr, and DPE elements. (wikiversity.org)
  • CBL-70Z deregulates the cellular tyrosine kinase machinery, as NIH3T3 serum-starved cells expressing CBL-70Z showed significantly increased endothelial growth factor receptor (EGFR) kinase activity after EGF stimulation ( 5 ). (aacrjournals.org)
  • GH receptor (GHR), GH-releasing hormone receptor (GHRHR), and five somatostatin receptors (SSTR1-5) bind their respective ligands and regulate their function ( 6 ). (aacrjournals.org)
  • We dissect the mechanism of SARS-CoV-2 in human lung host from the initial phase of receptor binding to viral replication machinery. (frontiersin.org)
  • Our study establishes the structural basis of the AtERF96 transcription factor in complex with the GCC box, as well as the DNA binding mechanisms of the N-terminal α-helix and AP2/ERF domain. (springer.com)
  • In adult human serum, most of IGFs are bound in a 150 kDa complex which could not cross the capillary wall. (core.ac.uk)
  • Furthermore, artificial connections between DNA binding domains and either GTFs or components of the RNAPII holoenzyme complex stimulate transcription (reviewed in ref. 1 ), yet tethering of an activation domain to GTFs or RNAPII components fails to stimulate transcription ( 9 ). (pnas.org)
  • The role of the core-binding factor ß (CBFß) subunit in the CBF heterodimeric complex. (dartmouth.edu)
  • in an essential splicing factor complex. (rochester.edu)
  • Most of the circulating IGF1 is bound in a ternary complex with IGFBP3 and the acid-labile subunit (ALS). (aacrjournals.org)
  • These structures are essential factors in determining the mechanism of translation, but information about how the ribosome assembles into a stable multi-component complex is still very limited. (wikibooks.org)
  • The binding of IGFs to IGF1R stimulates the receptor's tyrosine kinase activity, which activates downstream signaling pathways, including the PI3K-AKT-TOR and RAF-MAPK systems. (aacrjournals.org)
  • We analyzed the binding residues of the conserved AP2/ERF domain in the DNA recognition sequence. (springer.com)
  • In this workflow we demonstrate Bioconductor techniques for finding candidate TF binding sites in DNA sequence using the model organism Saccharomyces cerevisiae. (bioconductor.org)
  • Search tools and flat file downloads are provided to retrieve binding site information (as sequences, matrices and sequence logos) for individual TFs, groups of TFs or for all TFs with characterized binding specificities. (umassmed.edu)
  • We show that TF-specific models based on the sequence or DNA shape of the regions flanking the core binding site are highly predictive of the measured differential TF binding. (weizmann.ac.il)
  • A TATA box is a common type of core promoter sequence in eukaryotes which is a short DNA sequence. (wikiversity.org)
  • ITC analysis of ligand binding to preQ? (rochester.edu)
  • Single transcriptional and translational preQ1 riboswitches adopt similar pre-folded ensembles that follow distinct folding pathways into the same ligand-bound structure. (rochester.edu)
  • Although MTX includes a lower Significantly affinity, its make use of being a ligand continues to be effective for Significantly concentrating on if a multivalent style AST-1306 strategy [28,29,30] is certainly applied that may offer very tight binding in comparison to a weak monovalent binding interaction. (healthandwellnesssource.org)
  • Retinoid receptors (RARs and RXRs) are ligand-dependent transcription factors. (mdpi.com)
  • FlyFactorSurvey is a database of DNA binding specificities for Drosophila transcription factors (TFs) primarily determined using the bacterial one-hybrid system. (umassmed.edu)
  • We here report the outcome of AML patients without core-binding factor mutation (non-CBF AML) in the AML-05 study. (fujita-hu.ac.jp)
  • The insulin-like growth factor (IGF) signaling pathway plays an important role in regulating cellular proliferation and apoptosis ( 1 ). (aacrjournals.org)
  • Isolation and properties of cDNA clones encoding SRF, a transcription factor that binds to the c-fos serum response element. (ebi.ac.uk)
  • Transcription factor binding to promoter DNA sequences is a stochastic process, and imperfect matches can be sufficient for binding. (bioconductor.org)
  • Many DNA variants associated with diseases belong to sequences that evidently control the activity of transcription factors. (mpg.de)
  • Here, we present a novel experimental assay termed BunDLE-seq that provides quantitative measurements of TF binding to thousands of fully designed sequences of 200 bp in length within a single experiment. (weizmann.ac.il)
  • Applying this binding assay to two yeast TFs we demonstrate that sequences outside the core TF binding site profoundly affect TF binding. (weizmann.ac.il)
  • 8) In general, the cytogenetic landscape of AML is characterized either by balanced chromosomal rearrangements, often involving hematopoietic transcription factors (TFs), or by chromosomal losses and gains similar to those present in MDS. (thefreelibrary.com)
  • Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. (abcam.com)
  • AP2/ERFs contain a highly conserved DNA-binding domain consisting of approximately 60 amino acids (Nakano et al. (springer.com)
  • A core domain of around 90 amino acids is sufficient for the activities of DNA-binding, dimerisation and interaction with accessory factors. (ebi.ac.uk)
  • In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. (nih.gov)
  • Various extensions to this model have been proposed, most of which take account of dependencies between the bases in the binding sites. (core.ac.uk)
  • In some cases this variation is known to affect the function of binding sites. (core.ac.uk)
  • ud Results: We re-analysed binding sites from the TRANSFAC database and found motivating examples where our new variable length model provides a better fit. (core.ac.uk)
  • ud Conclusions: We have presented a new gapped PWM model for variable length DNA binding sites that is not too restrictive nor over-parameterised. (core.ac.uk)
  • 2000). DNA binding sites: representation and discovery. (core.ac.uk)
  • Using this site-specific ChIP, we were able to confirm four previously described Sp1 binding sites within egfr promoter region to be occupied by Sp1 in vivo. (biomedsearch.com)
  • Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. (uniprot.org)
  • The TβRE contains two CBF binding sites. (jimmunol.org)
  • they only flank the binding sites. (mpg.de)
  • We further characterize the dependence of TF binding, accounting for measurements of single and co-occurring binding events, on the number and location of binding sites and on the TF concentration. (weizmann.ac.il)
  • U.S.A. 103, 13676−13681], the binding sites for excess nickel ions have not been fully described. (harvard.edu)
  • These anomalous data show that excess nickel ions do not bind to a single location on NikR but instead reveal a total of 22 possible low-affinity nickel sites on the NikR tetramer. (harvard.edu)
  • No Binding sites available for this model. (genereg.net)