Core Binding Factors: Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor beta Subunit: A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor alpha Subunits: A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor Alpha 2 Subunit: A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.Core Binding Factor Alpha 1 Subunit: A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.Transcription Factor AP-2: A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.Smooth Muscle Myosins: Myosin type II isoforms found in smooth muscle.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Chromosomes, Human, Pair 16: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.Chromosome Inversion: An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Oncogene Proteins, Fusion: The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.Chromosomes, Human, Pair 21: A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Enhancer Elements, Genetic: Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Translocation, Genetic: A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Osteocalcin: Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Leukemia, Myeloid: Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.Pol1 Transcription Initiation Complex Proteins: Factors that form a preinitiation complex at promoters that are specifically transcribed by RNA POLYMERASE I.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 188.8.131.52.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Core Binding Factor Alpha 3 Subunit: A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Telomeric Repeat Binding Protein 1: A ubiquitously expressed telomere-binding protein that is present at TELOMERES throughout the CELL CYCLE. It is a suppressor of telomere elongation and may be involved in stabilization of telomere length. It is structurally different from TELOMERIC REPEAT BINDING PROTEIN 2 in that it contains acidic N-terminal amino acid residues.G-Box Binding Factors: A family of transcription factors found primarily in PLANTS that bind to the G-box DNA sequence CACGTG or to a consensus sequence CANNTG.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Access to Information: Individual's rights to obtain and use information collected or generated by others.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Sodium Fluoride: A source of inorganic fluoride which is used topically to prevent dental caries.Fluorides: Inorganic salts of hydrofluoric acid, HF, in which the fluorine atom is in the -1 oxidation state. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed) Sodium and stannous salts are commonly used in dentifrices.Fluoride PoisoningDepsipeptides: Compounds consisting of chains of AMINO ACIDS alternating with CARBOXYLIC ACIDS via ester and amide linkages. They are commonly cyclized.Leukemia, Lymphocytic, Chronic, B-Cell: A chronic leukemia characterized by abnormal B-lymphocytes and often generalized lymphadenopathy. In patients presenting predominately with blood and bone marrow involvement it is called chronic lymphocytic leukemia (CLL); in those predominately with enlarged lymph nodes it is called small lymphocytic lymphoma. These terms represent spectrums of the same disease.Histone Deacetylase Inhibitors: Compounds that inhibit HISTONE DEACETYLASES. This class of drugs may influence gene expression by increasing the level of acetylated HISTONES in specific CHROMATIN domains.Cytarabine: A pyrimidine nucleoside analog that is used mainly in the treatment of leukemia, especially acute non-lymphoblastic leukemia. Cytarabine is an antimetabolite antineoplastic agent that inhibits the synthesis of DNA. Its actions are specific for the S phase of the cell cycle. It also has antiviral and immunosuppressant properties. (From Martindale, The Extra Pharmacopoeia, 30th ed, p472)Dictionaries, MedicalLeukemia Virus, Murine: Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.
FIRE3 in the promoter of the rat fatty acid synthase (FAS) gene binds the ubiquitous transcription factors CBF and USF but does not mediate an insulin response in a rat hepatoma cell line. (1/96)Several putative insulin-responsive elements (IRE) in the fatty acid synthase (FAS) promoter have been identified and shown to be functional in adipocytes and hepatocytes. Here we report on the insulin-responsiveness in the rat hepatoma cell line H4IIE of four cis-elements in the FAS promoter: the FAS insulin-responsive elements, FIRE2 and FIRE3; the inverted CCAAT element, ICE; and the insulin/glucose-binding element, designated hepatic FIRE element, hFIRE, originally identified in rat hepatocytes. Using electrophoretic mobility shift assay (EMSA) competition experiments together with supershifts and in vitro transcription/translation we show that FIRE3 (-68/-58) binds not only the upstream stimulatory factors USF-1/USF-2 but also the CCAAT-binding factor CBF, also known as the nuclear factor Y, NF-Y. The putative IRE FIRE2, which shows sequence similarity to FIRE3, is located between -267 and -249. Gel retardation experiments indicate that USF-1 and USF-2 also bind to this element, which contains an imperfect E-box motif. Using the same approach we have shown that hFIRE binds the stimulatory proteins Sp1 and Sp3 in addition to CBF. Transient transfection experiments using FAS promoter constructs deleted for FIRE2 and FIRE3 demonstrate that neither of these elements mediates the insulin response of the FAS promoter in the rat hepatoma cell line H4IIE, however, ICE at -103/-87 is responsible for mediating the effect of the insulin antagonist cAMP. The hFIRE element located at -57/-34, in spite of its role in the glucose/insulin response in primary rat hepatocytes, is apparently not involved in the insulin regulation of the rat FAS promoter in H4IIE cells. The fact that the topology of the promoters of the FAS genes in rat, human, goose and chicken is conserved regarding CBF-binding sites and USF-binding sites implies an important role for these ubiquitously expressed transcription factors in the regulation of the FAS promoter. (+info)
Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation. (2/96)Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene family that is expressed at high levels in diverse human carcinomas and in articular cartilage from arthritic patients. In addition to its expression in pathological conditions, collagenase 3 has been detected in osteoblasts and hypertrophic chondrocytes during fetal ossification. In this work, we have evaluated the possibility that Cbfa1 (core binding factor 1), a transcription factor playing a major role in the expression of osteoblastic specific genes, is involved in the expression of collagenase 3 during bone formation. We have functionally characterized a Cbfa motif present in the promoter region of collagenase 3 gene and demonstrated, by cotransfection experiments and gel mobility shift assays, that this element is involved in the inducibility of the collagenase 3 promoter by Cbfa1 in osteoblastic and chondrocytic cells. Furthermore, overexpression of Cbfa1 in osteoblastic cells unable to produce collagenase 3 leads to the expression of this gene after stimulation with transforming growth factor beta. Finally, we show that mutant mice deficient in Cbfa1, lacking mature osteoblasts but containing hypertrophic chondrocytes which are also a major source of collagenase 3, do not express this protease during fetal development. These results provide in vivo evidence that collagenase 3 is a target of the transcriptional activator Cbfa1 in these cells. On the basis of these transcriptional regulation studies, together with the potent proteolytic activity of collagenase 3 on diverse collagenous and noncollagenous bone and cartilage components, we proposed that this enzyme may play a key role in the process of bone formation and remodeling. (+info)
Does adult fracture repair recapitulate embryonic skeletal formation? (3/96)Bone formation is a continuous process that begins during fetal development and persists throughout life as a remodeling process. In the event of injury, bones heal by generating new bone rather than scar tissue; thus, it can accurately be described as a regenerative process. To elucidate the extent to which fetal skeletal development and skeletal regeneration are similar, we performed a series of detailed expression analyses using a number of genes that regulate key stages of endochondral ossification. They included genes in the indian hedgehog (ihh) and core binding factor 1 (cbfa1) pathways, and genes associated with extracellular matrix remodeling and vascular invasion including vascular endothelial growth factor (VEGF) and matrix metalloproteinase 13 (mmp13). Our analyses suggested that even at the earliest stages of mesenchymal cell condensation, chondrocyte (ihh, cbfa1 and collagen type II-positive) and perichondrial (gli1 and osteocalcin-positive) cell populations were already specified. As chondrocytes matured, they continued to express cbfa1 and ihh whereas cbfa1, osteocalcin and gli1 persisted in presumptive periosteal cells. Later, VEGF and mmp13 transcripts were abundant in chondrocytes as they underwent hypertrophy and terminal differentiation. Based on these expression patterns and available genetic data, we propose a model where Ihh and Cbfa1, together with Gli1 and Osteocalcin participate in establishing reciprocal signal site of injury. The persistence of cbfa1 and ihh, and their targets osteocalcin and gli1, in the callus suggests comparable processes of chondrocyte maturation and specification of a neo-perichondrium occur following injury. VEGF and mmp13 are expressed during the later stages of healing, coincident with the onset of vascularization of the callus and subsequent ossification. Taken together, these data suggest the genetic mechanisms regulating fetal skeletogenesis also regulate adult skeletal regeneration, and point to important regulators of angiogenesis and ossification in bone regeneration. (+info)
Mutation analysis of core binding factor A1 in patients with cleidocranial dysplasia. (4/96)Cleidocranial dysplasia (CCD) is a dominantly inherited disorder characterized by patent fontanelles, wide cranial sutures, hypoplasia of clavicles, short stature, supernumerary teeth, and other skeletal anomalies. We recently demonstrated that mutations in the transcription factor CBFA1, on chromosome 6p21, are associated with CCD. We have now analyzed the CBFA1 gene in 42 unrelated patients with CCD. In 18 patients, mutations were detected in the coding region of the CBFA1 gene, including 8 frameshift, 2 nonsense, and 9 missense mutations, as well as 2 novel polymorphisms. A cluster of missense mutations at arginine 225 (R225) identifies this residue as crucial for CBFA1 function. In vitro green fluorescent protein fusion studies show that R225 mutations interfere with nuclear accumulation of CBFA1 protein. There is no phenotypic difference between patients with deletions or frameshifts and those with other intragenic mutations, suggesting that CCD is generally caused by haploinsufficiency. However, we were able to extend the CCD phenotypic spectrum. A missense mutation identified in one family with supernumerary teeth and a radiologically normal skeleton indicates that mutations in CBFA1 can be associated exclusively with a dental phenotype. In addition, one patient with severe CCD and a frameshift mutation in codon 402 had osteoporosis leading to recurrent bone fractures and scoliosis, providing first evidence that CBFA1 may help maintain adult bone, in addition to its function in bone development. (+info)
A full-length Cbfa1 gene product perturbs T-cell development and promotes lymphomagenesis in synergy with myc. (5/96)The Cbfa1/PEBP2 alpha A/AML3 gene plays an essential role in osteogenesis but is also expressed in the T-cell lineage where it has been implicated in lymphoma development as a target for retroviral insertional mutagenesis. As lymphoma cells with til-1 insertion express at least five distinct Cbfa1 isoforms, it is important to establish which, if any, have intrinsic oncogenic potential. We have generated transgenic mice in which the most abundant lymphoma isoform (G1/p57) is expressed under the control of the CD2 locus control region. Co-precipitation analysis of transgenic thymus revealed high levels of Cbfa1 protein in an abundant complex containing the binding cofactor Cbfb. CD2-Cbfa1-G1 mice displayed abnormal T-cell development, with a pronounced skew towards CD8 SP cells in the thymus and developed a low incidence of spontaneous lymphomas (6% at 12 months) with cells of similar phenotype. Strongly synergistic tumour development was seen when CD2-Cbfa1-G1 mice were crossed with lines carrying myc transgenes (CD2-myc or tamoxifen-regulatable CD2-mycER) and Cbfa1 was found to rescue expression of the CD2-myc transgene in pre-leukaemic mice. However, synergy did not appear to be due to a dominant block of myc-induced apoptosis by Cbfa1 as explanted primary tumours and cell lines from CD2-Cbfa1-G1/CD2-mycER mice showed accelerated death on induction with tamoxifen at similar rates to CD2-mycER controls. Moreover, thymocytes from preleukaemic CD2-Cbfa1-G1 mice showed reduced survival in vitro and increased sensitivity to the inhibitory effects of TGF-beta. This study demonstrates that a full-length Cbf alpha-chain gene can act as an oncogene without fusion to a heterologous protein. (+info)
Parathyroid hormone regulation of the rat collagenase-3 promoter by protein kinase A-dependent transactivation of core binding factor alpha1. (6/96)Previously we showed that the activator protein-1 site and the runt domain binding site in the collagenase-3 promoter act cooperatively in response to parathyroid hormone (PTH) in the rat osteoblastic osteosarcoma cell line, UMR 106-01. Our results of the expression pattern of core binding factor alpha1 (Cbfa1), which binds to the runt domain site, indicated that there is no change in the levels of Cbfa1 protein or RNA under either control conditions or after PTH treatment. The importance of posttranslational modification of Cbfa1 in the signaling pathway for PTH-induced collagenase-3 promoter activity was analyzed. PTH stimulation of collagenase-3 promoter activity was completely abrogated by protein kinase A (PKA) inhibition. To determine the role of PKA activity with respect to Cbfa1 activation (in addition to its known activity of phosphorylating cAMP-response element-binding protein to enhance c-fos promoter activity), we utilized the heterologous Gal4 transcription system. PTH stimulated the transactivation of activation domain-3 in Cbfa1 through the PKA site. In vitro phosphorylation studies indicated that the PKA site in the wild type activation domain-3 is a substrate for phosphorylation by PKA. Thus, we demonstrate that PTH induces a PKA-dependent transactivation of Cbfa1, and this transactivation is required for collagenase-3 promoter activity in UMR cells. (+info)
An osteogenesis-related transcription factor, core-binding factor A1, is constitutively expressed in the chondrocytic cell line TC6, and its expression is upregulated by bone morphogenetic protein-2. (7/96)Core-binding factor A1 (Cbfa1), also called Pebp2 alpha A/AML3, is a transcription factor that belongs to the runt-domain gene family. Cbfa1-deficient mice are completely incapable of both endochondral and intramembranous bone formation, indicating that Cbfa1 is indispensable for osteogenesis. Maturation of chondrocytes in these mice is also disorganized, suggesting that Cbfa1 may also play a role in chondrogenesis. The aim of this study was to examine the expression and regulation of Pebp2 alpha A/AML3/Cbfa1 expression in the chondrocyte-like cell line, TC6. Northern blot analysis indicated that Cbfa1 mRNA was constitutively expressed as a 6.3 kb message in TC6 cells and the level of Cbfa1 expression was enhanced by treatment with bone morphogenetic protein-2 (BMP2) in a time- and dose-dependent manner. This effect was blocked by an RNA polymerase inhibitor, 5,6-dichloro-1-beta-d-ribofuranosylbenzimidazole, but not by a protein synthesis inhibitor, cycloheximide. Western blot analysis of the cell lysates using polyclonal antibody raised against Cbfa1 indicated that BMP2 treatment increased the Cbfa1 protein level in TC6 cells. In TC6 cells, BMP2 treatment enhanced expression of alkaline phosphatase and type I collagen mRNAs but suppressed that of type II collagen mRNA. In addition to TC6 cells, Cbfa1 mRNA was also expressed in primary cultures of chondrocytes and BMP2 treatment enhanced Cbfa1 mRNA expression in these cells similarly to its effect on TC6 cells. These data indicate that the Pebp2 alpha A/AML3/Cbfa1 gene is expressed in a chondrocyte-like cell line, TC6, and its expression is enhanced by treatment with BMP. (+info)
Exogenous cdk4 overcomes reduced cdk4 RNA and inhibition of G1 progression in hematopoietic cells expressing a dominant-negative CBF - a model for overcoming inhibition of proliferation by CBF oncoproteins. (8/96)Core Binding Factor (CBF) is required for the development of definitive hematopoiesis, and the CBF oncoproteins AML1-ETO, TEL-AML1, and CBFbeta-SMMHC are commonly expressed in subsets of acute leukemia. CBFbeta-SMMHC slows the G1 to S cell cycle transition in hematopoietic cells, but the mechanism of this effect is uncertain. We have sought to determine whether inhibition of CBF-mediated trans-activation is sufficient to slow proliferation. We demonstrate that activation of KRAB-AML1-ER, a protein containing the AML1 DNA-binding domain, the KRAB repression domain, and the Estrogen receptor ligand binding domain, also slows G1, if its DNA-binding domain is intact. Also, exogenous AML1 overcame CBFbeta-SMMHC-induced inhibition of proliferation. Representational difference analysis (RDA) identified cdk4 RNA expression as an early target of KRAB-AML1 activation. Inhibition of CBF activities by KRAB-AML1-ER or CBFbeta-SMMHC rapidly reduced endogenous cdk4 mRNA levels, even in cells proliferating at or near control rates as a result of exogenous cdk4 expression. Over-expression of cdk4, especially a variant which cannot bind p16INK4a, overcame cell cycle inhibition resulting from activation of KRAB-AML1-ER, although cdk4 did not accelerate proliferation when expressed alone. These findings indicate that mutations which alter the expression of G1 regulatory proteins can overcome inhibition of proliferation by CBF oncoproteins. Oncogene (2000). (+info)
Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. Few prognostic markers have been identified. In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. Overall, 10-year overall survival (OS), disease-free survival (DFS), and event-free survival were 63.9%, 54.8%, and 49.9%, respectively; patients with the t(8;21) and inv(16) chromosomal rearrangements exhibited significant differences at diagnosis. Despite similarly high complete remission (CR) rate, patients with inv(16) experienced superior DFS and a high chance of achieving a second CR, often leading to prolonged OS also after relapse. We found that a complex karyotype (ie, ≥4 cytogenetic anomalies) affected survival; the KIT D816 mutation predicted worse prognosis only in patients with the t(8;21) rearrangement, whereas FLT3 mutations had no ...
Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
This study is examining the appropriate dose and side effects of dasatinib, when it is given with the standard of care chemotherapy for children and adolescents
KIT is a receptor tyrosine kinase that is functionally relevant for hematopoiesis, mast cell development and function, gametogenesis and melanogenesis. Normal KIT signaling requires binding to stem cell factor, and PI3K-Akt is one of the putative effector pathways. In humans, germline loss-of-function KIT mutations have been associated with piebaldism - an autosomal dominant condition characterized by depigmented patches of skin and hair. Gain-of-function KIT mutations are usually acquired and have been associated with myeloid malignancies including core binding factor acute myeloid leukemia and systemic mastocytosis (SM), germ cell tumors, gastrointestinal stromal tumors and sinonasal T cell lymphomas. KITD816V is the most prevalent KIT mutation in mast cell disease and occurs in more than 90% of the cases that fulfill the World Health Organization diagnostic criteria for SM. However, its precise pathogenetic contribution is not well understood. In clinical practice, SM is considered either indolent or
core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
TY - JOUR. T1 - Selection of reversions and suppressors of a mutation in the CBF binding site of a lymphomagenic retrovirus. AU - Martiney, Marita J.. AU - Rulli, Karen. AU - Beaty, Robert. AU - Levy, Laura S.. AU - Lenz, Jack. PY - 1999/8/23. Y1 - 1999/8/23. N2 - The retrovirus SL3 induces T-cell lymphomas in mice. The transcriptional enhancer in the long terminal repeat (LTR) of SL3 contains two 72-bp repeats. Each repeat contains a binding site for the transcription factor CBF (also called AML1). The CBF binding sites are called core elements. SAA is a mutant that is identical to SL3 except for the presence of a single-base-pair substitution in each of the two core elements. This mutation significantly attenuates viral lymphomagenicity. Most lymphomas that occur in SAA-infected mice contain proviruses with reversions or second-site suppressor mutations within the core element. We examined the selective pressures that might account for the predominance of the reversions and suppressor ...
The purpose of the study is to determine whether Fludarabine in combination with cytarabine is more effective than high-dose cytarabine in post-remission
PRIMARY OBJECTIVES:. I. To assess the safety and tolerability of dasatinib with intensive induction therapy (daunorubicin hydrochloride and cytarabine), consolidation chemotherapy (high-dose cytarabine), and as single agent in maintenance therapy in patients with newly diagnosed core-binding factor acute myeloid leukemia (AML).. SECONDARY OBJECTIVES:. I. To assess clinical outcomes such as event-free survival (EFS), complete response (CR) rate, cumulative incidence of relapse (CIR), cumulative incidence of death (CID), disease-free survival (DFS), and overall survival (OS) of patients treated with these regimens.. II. To describe the frequency and severity of adverse events of patients treated on this study during induction, consolidation, and continuation therapy.. III. To describe the interaction of pretreatment disease and patient characteristics including morphology, cytogenetics, immunophenotype, molecular genetic features, white blood cell (WBC) count and hemogram, and performance status ...
The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
Among patients with good prognosis core binding factor AML, there is an overall survival rate of only 44%. To understand the genetic factors contributing to poor outcomes within this subgroup, Dr Chew is analysing bone marrow samples collected from 18 patients before and during treatment.. According to Dr Chew, multiple genetic abnormalities acquired during therapy are probably responsible for good prognosis core binding AML developing resistance to chemotherapy.. "To help us predict who will respond poorly to therapy, were identifying the genetic mutations occurring in patients who relapse," he said. "This information will allow us to tailor patient treatment accordingly. Currently a stem cell transplant is considered the definitive treatment and our findings will help clinicians decide if their patients AML will develop resistance and if a stem cell transplant is recommended.". Dr Chew is testing the usefulness of the genetic variations he identifies through an international ...
Disease, Muscle, Muscle Cells, Smooth Muscle, Smooth Muscle Cells, Cells, Heme, Hydrogen, Alkaline Phosphatase, Kidney, Kidney Disease, Osteocalcin, Upregulation, Vascular Calcification, Calcium, Heme Oxygenase, Vascular Smooth Muscle, Atherosclerosis, Bone, Core Binding Factor
This phase II study of romidepsin in relapsed and refractory AML shows that this HDACI has a differential antileukemic activity in patients with CBF AML [predominantly t(8;21)] and that this was associated with an up-regulation of AML1 target genes. These findings are in keeping with observations in vitro that have established that t(8;21) AML is sensitive to therapy with various HDACIs including romidepsin (7, 16-19, 27). Despite the in vitro evidence suggesting an inherent sensitivity of this cytogenetic subset of AML to this class of agents, this is the first study to actually show antileukemic activity in vivo in a cohort of patients with t(8;21) AML and other translocations involving the AML1 gene.. Romidepsin has been investigated in a phase I trial (28) conducted at this dose and schedule in two groups of patients with chronic lymphocytic leukemia and AML, respectively. In that trial that involved 10 AML patients, there were no patients with CBF AML. There were no objective responses ...
Cells, Lead, Acute Myeloid Leukemia, Bone, Bone Marrow, Core-binding Factor, Cytogenetic, Cytogenetic Abnormalities, Disease, Leukemia, Marrow, Myeloid Leukemia, Patient, Patients, Protein Array, Proteins, Regression, Regression Analysis, Ability, Algorithm
Looking for online definition of core-binding factor, runt domain, alpha subunit 3 in the Medical Dictionary? core-binding factor, runt domain, alpha subunit 3 explanation free. What is core-binding factor, runt domain, alpha subunit 3? Meaning of core-binding factor, runt domain, alpha subunit 3 medical term. What does core-binding factor, runt domain, alpha subunit 3 mean?
As per available reports about 248 journals, 14 Conferences and workshops are presently dedicated exclusively to Acute Respiratory Failure and about 1
To study the effects and importance of fluoride on FBs in the development of extraperiosteal calcification and the ossification of skeletal fluorosis, the presence of the osteogenic phenotype, which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in osteoblasts (OBs) exposed to fluoride was determined. Fibroblasts and osteoblasts were exposed to different concentrations of fluoride (0, 0.0001, 0.001, 0.1, 1.0, 10.0 and 20.0mg/L F-). By using RT-PCR and ELISA, the mRNA levels of Cbfa1 and OCN were measured at 48h, and the protein levels of Cbfa1 and OCN were measured at 2, 4, 24, 48 and 72h. The data demonstrated the following: (1) The Cbfa1 protein level in fluoride-treated fibroblasts clearly increased at 48h in the groups treated with 0.0001, 0.001, 0.1, 1.0 and 20.0mg/L F-. The Cbfa1 protein level of the group treated with 10mg/L F- at 72h was higher than that of the control group. The level of Cbfa1 mRNA in the fibroblasts ...
TY - JOUR. T1 - HDAC1 is a required cofactor of CBFb-SMMHC and a potential therapeutic target in inversion 16 acute myeloid leukemia. AU - Richter, Lisa E.. AU - Wang, Yiqian. AU - Becker, Michelle E.. AU - Coburn, Rachel A.. AU - Williams, Jacob T.. AU - Amador, Catalina. AU - Hyde, R. Katherine. PY - 2019/6/1. Y1 - 2019/6/1. N2 - Acute myeloid leukemia (AML) is a neoplastic disease characterized by the uncontrolled proliferation and accumulation of immature myeloid cells. A common mutation in AML is the inversion of chromosome 16 [inv (16)], which generates a fusion between the genes for core binding factor beta (CBFB) and smooth muscle myosin heavy chain gene (MYH11), forming the oncogene CBFB-MYH11. The expressed protein, CBFb-SMMHC, forms a heterodimer with the key hematopoietic transcription factor RUNX1. Although CBFb-SMMHC was previously thought to dominantly repress RUNX1, recent work suggests that CBFb-SMMHC functions together with RUNX1 to activate transcription of specific target ...
The novel FMS-like tyrosine kinase 3 (FLT3)-N676K point mutation within the FLT3 kinase domain-1 was recently identified in 6 % of de novo acute myeloid leukemia (AML) patients with inv(16). Because FLT3-N676K was encountered almost exclusively in inv(16) AML, we investigated the transforming potential of FLT3-N676K, the cooperation between FLT3-N676K and core binding factor ß-smooth muscle myosin heavy chain (CBFß-SMMHC) (encoded by the inv(16) chimeric gene CBFB-MYH11) in inducing acute leukemia, and tested the sensitivity of FLT3-N676K-positive leukemic cells to FLT3 inhibitors. Retroviral expression of FLT3-N676K in myeloid 32D cells induced AML in syngeneic C3H/HeJ mice (n = 11/13, median latency 58 days), with a transforming activity similar to FLT3-internal tandem duplication (ITD) (n = 8/8), FLT3-TKD D835Y (n = 8/9), and FLT3-ITD-N676K (n = 9/9) mutations. Three out of 14 (21.4 %) C57BL/6J mice transplanted with FLT3-N676K-transduced primary hematopoietic progenitor cells developed ...
Core binding factor (CBF) is a heterodimeric transcription factor that is essential for a number of developmental process including hemotopoiesis and bone development. CBFs contain a DNA-binding CBFα. subunit and a non-DNA binding CBFß. subunit ...
The pathogenesis of PDAC involves genetic alterations, such as K-ras protooncogene mutations, mutations of the p53, p16, and Smad4 tumour suppressor genes, and other less common mutations.2 In addition, there are numerous epigenetic alterations, including altered expression of several growth factors and their receptors.3 For example, PDACs overexpress all TGFβ isoforms and their receptors, and overexpression of these ligands and receptors is often associated with shortened postoperative survival of patients with pancreatic cancer.14,15. The TGFβ pathway is carefully regulated, with Smad proteins as the key component in the signal transduction pathway. In addition, other regulators, such as transcription factors, which facilitate Smad binding to target promoters, may provide routes for feedback and crosstalk.16 For example, members of the CBFA (core binding factor A) family of transcription factors act both as targets and partners of activated Smads. This family, also termed the "Runx family", ...
According to Dr. Stone, MRD status in CBF-AML might also make a difference in treatment selection. A separate study of patients with CBF-AML found that those with an MRD level of at least 1% at one point following induction therapy experienced relapse 90% of the time, with a median remission duration of 10 months (P , .001).4 "This is one group of patients we could justify transplanting," Dr. Stone observed, "but that doesnt mean its going to make a difference.". MRD test results have also been shown to improve outcome prediction,5 albeit modestly, when included with more typical clinical data (ie, age, gender, performance status, white blood cell count, platelet count, bone marrow blast percentage, cytogenetic risk, and NPM1/FLT3-ITD status). Because predictive outcome was only improved by 3%, however, MRD testing is not worth the effort for that reason alone, according to Dr. Stone.. Testing Limitations. Whether performed with multiparameter flow cytometry or next-generation sequencing, MRD ...
Decreased Llgl1 expression has been previously shown to be associated with increased metastatic potential, malignant phenotype, or inferior survival in a variety of solid tumors (Ohali et al., 2004; Schimanski et al., 2005; Kuphal et al., 2006). Given that Llgl1 restrains self-renewal in HSCs and that leukemia is characterized by aberrant activation of self-renewal, we assessed for a role in human AML. First, we asked whether the gene expression signature revealed by genetic inactivation of Llgl1 (Llgl1−/− signature) in HSCs was associated with any genetic subtypes of primary AML. Indeed, clustering based on the Llgl1−/− signature (Fig. 4 B) grouped AML cases into subgroups significantly associated with cytogenetic abnormalities (Fig. 4, B and C). Especially core-binding factor leukemias (t(8;21) and inv(16)) or t(15;17) clustered into specific subgroups. Moreover, the Llgl1−/− signature was most strongly correlated with group 7 based on hierarchical clustering. This group contained ...
Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. Runx proteins are encoded by three genes: Runx1, Runx2 , and ...
Complete information for CBFB gene (Protein Coding), Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
A series of studies of f-electron systems based on density functional theory methods have been performed. The focus of the studies has been on magnetic and structural properties, as well as investigating ways to handle strong electron correlation in these systems.. A version of the self-interaction correction (SIC) method has been developed for a full-potential linear muffin-tin orbital method. The method is demonstrated to have the strong capabilities of previous SIC implementations, to study energetics and phase stabilities of d- and f-electron systems with localisation-delocalisation transitions, but with no geometrical constraints from the underlying band structure method. The method is applied to the high-TC superconductor CeOFeAs, in which the f-shell of the Ce atoms is argued to undergo a Mott transition to a delocalised state under pressure.. The non-collinear magnetic structures of two rare earth compounds, TbNi5 and CeRhIn5 have been studied, and in both cases the complex magnetic ...
Saturday, October 17, 1953 ld Blinds The Daily Register-Mail, Galesburg, Til- Saturday, October 17, 1953 , 8 GALVA-Meeting of duck hunters of Lake Calhoun Association was held Thursday night at the clubhouse. Plans were made for those Interested to meet at 8 oclock Sunday morning at the clubhouse to build blinds for the hu. Edition of Galesburg Register-Mail
PHP & Kejuruteraan Perisian Projects for $250 - $750. Prescription Pad Writing Software contrive by CompuRx Infotech Pvt. Ltd. is having the ability to check for drug safety at individual disease level like in case of G6PD(40 drugs contraindicated), POR...
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two ...
Autor: Otto, Florian et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2002-02-13; Keywords: cleidocranial dysplasia; CCD; transcription factor; core binding factor; runt domain; RUNX2; CBFA1; differentiation; osteoblast; Titel: Mutations in the RUNX2 gene in patients with cleidocranial dysplasia
TY - JOUR. T1 - Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. AU - North, Trista E.. AU - De Bruijn, Marella F T R. AU - Stacy, Terryl. AU - Talebian, Laleh. AU - Lind, Evan. AU - Robin, Catherine. AU - Binder, Michael. AU - Dzierzak, Elaine. AU - Speck, Nancy A.. PY - 2002. Y1 - 2002. N2 - Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1+/- embryos are heterogeneous and include CD45+ cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs among these various cell populations is sensitive to Runx1 dosage. These data provide the first morphological description of ...
Only the PBM motif is a classic HLH motif. Three different ChIP-chip-derived motifs are all diverse, but all score highly on ChIP-chip data! Are they motifs of other TFs? Check. 602: GCN4; 1095, TEC1; 1096: resembles 602, but is a closer match to CUP9/TOS8. Also hits GCN4. According to the literature (PMID: 9032238) the core binding site for the Rtg1p-Rtg3p heterodimer is 5-GGTCAC-3; the only motif that resembles this is 1446. Vague resemblance to 602 and 1096. I am going to retain 1446, which represents the literature site; PBM motif 870, which resembles an E-box, and ChIP-chip motif 1445, which scores highest on ChIP-chip data. But give all low confidence ...
Runx1 mediates the development of the granular convoluted tubules in the submandibular glands "The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription factor (RUNX) proteins (RUNX1, 2 and 3). This result clearly demonstrates that the Runx /Cbfb signaling pathway is indispensable in the development of the GCTs. However, it is not clear which of the RUNX proteins plays useful role in the development of the GCTs by activating the Runx /Cbfb signaling pathway. Past studies have revealed that the Runx /Cbfb signaling pathway plays important roles in various aspects of development and homeostatic events. Moreover, the Runx genes have different temporospatial ...
View mouse Terf2ip Chr8:112011398-112020528 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Translokacje chromosomalne modyfikujące Core-Binding Factor[edytuj , edytuj kod]. Ostra białaczka mielobastyczna z cechami ... Core-binding factor acute myeloid leukemia.. „Arch Pathol Lab Med". 135 (11), s. 1504-9, Nov 2011. DOI: 10.5858/arpa.2010-0482- ... Core binding factor genes and human leukemia. „Haematologica". 87 (12), s. 1307-23, Dec 2002. PMID: 12495904. ... Core-binding factor acute myeloid leukemia: can we improve on HiDAC consolidation?. „Hematology Am Soc Hematol Educ Program". ...
RNA polymerase II core promoter proximal region sequence-specific DNA binding. • chromatin binding. • transcription factor ... transcription factor activity, sequence-specific DNA binding. • mitogen-activated protein kinase p38 binding. • FK506 binding. ... DNA binding. • RNA polymerase II regulatory region sequence-specific DNA binding. • RNA polymerase II transcription factor ... protein binding. • transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding. • RNA polymerase ...
Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... Core binding factor acute myeloid leukaemia is a cancer related to genetic changes in the CBF gene. It is most commonly caused ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... Hart SM, Foroni L (2003). "Core binding factor genes and human leukemia". Haematologica. 87 (12): 1307-23. PMID 12495904. Bae ...
Runt-related transcription factor 1 (RUNX1) also known as acute myeloid leukemia 1 protein (AML1) or core-binding factor ... It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). ... but its DNA binding affinity is enhanced by 10 fold if it heterodimerises with the core binding factor β (CBFβ), also via the ... RUNX can behave as heterodimeric transcription factors collectively called the core binding factors (CBFs). The consensus ...
Bernués J, Espel E, Querol E (1986). "Identification of the core-histone-binding domains of HMG1 and HMG2". Biochim. Biophys. ... In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA ... After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 supports transcription of many genes in ... Contact with core histones changes the structure of nucleosomes. The presence of HMGB1 in the nucleus depends on ...
Binding to HS stabilizes fibroblast growth factors (FGFs) and vascular endothelial growth factors (VEGFs) and prevents them ... The basic HSPG structure consists of a protein core to which several linear heparin sulfate (HS) chains are covalently O-linked ... Growth factors such as epidermal growth factor (EGF), fibroblast growth factor (FGF) and transforming growth factor beta (TGF-β ... heparin-binding growth factors, chemokines and lipoproteins. HSPGs are prominent components of blood vessels. ...
... core protein binds to the growth factors BMP-4 and influences its bioactivity. It has also been reported that the ... There is also evidence that biglycan binds to TGF-beta 1. Biglycan interacts with collagen, both via the core protein and GAG ... Bidanset DJ, Guidry C, Rosenberg LC, Choi HU, Timpl R, Hook M (March 1992). "Binding of the proteoglycan decorin to collagen ... The structure of biglycan core protein is highly conserved across species; over 90% homology has been reported for rat, mouse, ...
"Entrez Gene: CBFA2T2 core-binding factor, runt domain, alpha subunit 2; translocated to, 2". Rual JF, Venkatesan K, Hao T, ... The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several ...
"Entrez Gene: CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3". Hoogeveen AT, Rossetti S, ... makes distinct contacts with multiple histone deacetylases and binds mSin3A through its oligomerization domain". Mol. Cell. ...
"New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ... The process is more complicated, and at least seven different factors are necessary for the binding of an RNA polymerase II to ...
Proneural proteins specifically bind DNA sequences that contain a core hexanucleotide motif, CANNTG, known as an E-box. The ... Because heterodimerization is a prerequisite for DNA binding, factors that interfere with dimerization effectively act as ... they express a membrane bound ligand, called ''Delta'', which binds and activate Notch receptors expressed in neighboring cells ... contacts between bHLH residues and DNA are responsible for the common ability of neural bHLH proteins to bind to the core E- ...
Makde R, England J, Yennawar H, Tan S (2010). "Structure of the RCC1 chromatin factor bound to the nucleosome core particle". ... is attached to nucleosomes via core histones H2A and H2B. Thus, a gradient of GTP-bound Ran is generated around the vicinity of ... which directly binds the growing ends of microtubules and coordinates the binding of other +TIPs. Opposing the action of these ... Once they bind a kinetochore, they are stabilized and their dynamics are reduced. The newly mono-oriented chromosome oscillates ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ... Binding and activation. Ligand binding is an equilibrium process. Ligands bind to receptors and dissociate from them ... Allosteric modulators: They do not bind to the agonist-binding site of the receptor but instead on specific allosteric binding ...
WRKY transcription factors have been a significant area of plant research for the past 20 years. The WRKY DNA-binding domain ... Eighteen amino acids are highly conserved in the WRKY protein domain, including the core motif, zinc-finger binding cysteines ... The WRKY domain is found in the WRKY transcription factor family, a class of transcription factors. The WRKY domain is found ... the evolutionary conserved DNA-binding specificities of WRKY transcription factors by molecular dynamics and in vitro binding ...
The TFs binding sites are physical DNA sites recognized by transcription factors within a genome. Note: Historically, binding ... Notes: Promoter sequences are specific to the different sigma factors associated to the RNA polymerase core. A promoter is ... An operon with several promoters located near each other may also have dual binding sites, indicating that such a site can ... The graphic display of a TU will always contain only one promoter -when known- with the binding sites that regulate its ...
"Fibroblast growth factor-binding protein is a novel partner for perlecan protein core". J. Biol. Chem. 276 (13): 10263-71. doi: ... The heparan sulfate chains of perlecan bind growth factors in the ECM, and serve as co-ligands or ligand enhancers when bound ... Transcriptional activation by transforming growth factor-beta via a nuclear factor 1-binding element". J. Biol. Chem. 272 (8): ... "The protein core of the proteoglycan perlecan binds specifically to fibroblast growth factor-7". J. Biol. Chem. 275 (10): 7095- ...
"Fibroblast growth factor-binding protein is a novel partner for perlecan protein core". J Biol Chem. 276 (13): 10263-71. doi: ... Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. FGFBP1, or HBP17, binds ... 2000). "Induction of the angiogenic modulator fibroblast growth factor-binding protein by epidermal growth factor is mediated ... "Characterization and molecular cloning of a putative binding protein for heparin-binding growth factors". J Biol Chem. 266 (25 ...
... factor binds to the core, forming the holoenzyme. After transcription starts, the factor can unbind and let the core enzyme ... In order to bind promoters, RNAP core associates with the transcription initiation factor sigma (σ) to form RNA polymerase ... RNA polymerase binding in bacteria involves the sigma factor recognizing the core promoter region containing the −35 and −10 ... A transcription factor and its associated transcription mediator complex must be attached to a DNA binding site called a ...
The degree to which the ETS1 transcription factor can bind to its binding sites on the PARP1 promoter depends on the ... helix DNA binding motif known as the Ets domain that specifically recognizes DNA sequences that contain a GGAA/T core element. ... Bi FF, Li D, Yang Q (2013). "Hypomethylation of ETS transcription factor binding sites and upregulation of PARP1 expression in ... The later suggests that several other transcription factors may facilitate Ets1 binding to unfavorable DNA sequences. Ets1 ...
The dimeric eukaryotic upstream binding factor (UBF) binds the UCE and the core element. UBF recruits and binds a protein ... allowing the UCE and the core elements to come into contact. RRN3/TIF-IA is phosphorylated and binds Pol I. Pol I binds to the ... When rRNA synthesis is stimulated, SL1 (selectivity factor 1) will bind to the promoters of rDNA genes that were previously ... Clos, Joachim; Buttgereit, Detlev; Grummt, Ingrid (February 1986). "A purified transcription factor (TIF-IB) binds to essential ...
FFAR2 transcription is regulated by the XBP1 transcription factor which binds to the core promoter. Mouse studies utilizing ... Brown AJ, Jupe S, Briscoe CP (2005). "A family of fatty acid binding receptors". DNA Cell Biol. 24 (1): 54-61. doi:10.1089/dna. ...
The protein encoded by this gene is an RNA binding protein and possible splicing factor. The encoded protein is found in the ... nucleus, where it colocalizes with core spliceosomal proteins. Studies of a mouse protein with high sequence similarity to this ... RNA-binding protein 39 is a protein that in humans is encoded by the RBM39 gene. ... "Entrez Gene: RBM39 RNA binding motif protein 39". Jung DJ, Na SY, Na DS, Lee JW (Jan 2002). "Molecular cloning and ...
In E. coli, a repressor binds the DNA operon and dislodges RNAP due to the loosely bound beta clamp, whereas in Synechocystis, ... Synechocystis possesses the 70S sigma factor (σ70), which can be divided into three groups. Group 1 sigma factors are critical ... Eubacterial RNAP holoenzymes consist of a core with four major subunits α2 ββ'. In cyanobacteria, β' is formed from two smaller ... the beta clamp binds tightly at initial binding. The effect of this difference is that synthetic repressible promoters do not ...
The core recommendations of this group took account of all psychological theory and spiritual factors in the human condition. " ... Service users who feel rejected are bound to escalate their behaviour in order to be heard and in order to "hit back" at those ... "Psychological mindedness" was the concept and the phrase that seemed to bind everything together. This work has led to the ... Conte HR, Ratto R, Karusa T (1996). "The Psychological Mindedness Scale: Factor structure and relationship to outcome of ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ... They do not bind to the agonist-binding site of the receptor but instead on specific allosteric binding sites, through which ... Ligand binding is an equilibrium process. Ligands bind to receptors and dissociate from them according to the law of mass ...
This model was said to be found in EU core countries such as Belgium, France, Germany, the Netherlands and Italy, and it is ... Trade unionism in the United Kingdom was a major factor in some of the economic crises during the 1960s and the 1970s, ... The agreements negotiated by a union are binding on the rank and file members and the employer and in some cases on other non- ... Ireland and the UK belong to this category, and in contrast to the EU core countries above, these countries first joined the EU ...
Core Binding Factor (CBF) Acute Myeloid Leukemia (AML) is cytogenetically characterized by either the t(8;21) or the inv(16)/t( ... Stem Cell Modeling of Core Binding Factor Acute Myeloid Leukemia. Federico Mosna and Michele Gottardi ...
In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age ... In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age ... Complex karyotype, older age, and reduced first-line dose intensity determine poor survival in core binding factor acute ... Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the ...
Dasatinib in Combination With Chemotherapy for Relapsed or Refractory Core Binding Factor Acute Myeloid Leukemia: A Phase I ... Dasatinib in Combination With Chemotherapy for Relapsed or Refractory Core Binding Factor Acute Myeloid Leukemia: A Phase I ...
SL3-3 enhancer factor 1 subunit beta. SL3/AKV core-binding factor beta subunit. core-binding factor beta subunit. polyomavirus ... CBFB core-binding factor subunit beta [Homo sapiens] CBFB core-binding factor subunit beta [Homo sapiens]. Gene ID:865 ... core-binding factor subunit beta. Names. CBF-beta. PEA2-beta. PEBP2-beta. SL3-3 enhancer factor 1 beta subunit. ... core-binding factor subunit betaprovided by HGNC. Primary source. HGNC:HGNC:1539 See related. Ensembl:ENSG00000067955 MIM: ...
OUTCOME OF CORE BINDING FACTOR ACUTE MYELOID LEUKEMIA IN ADULT PATIENTS. PB1727. El Gammal, M.1; Mahmoud, A.2; Samra, M.1; ... Home , June 2019 - Volume 3 - Issue , OUTCOME OF CORE BINDING FACTOR ACUTE MYELOID LEUKEMIA IN ADU... ... Core binding factor acute myeloid leukemia (CBF AML) encodes two recurrent cytogentic abnormalities, t(8;21) and inv (16) and ... OUTCOME OF CORE BINDING FACTOR ACUTE MYELOID LEUKEMIA IN ADULT PATIENTS: PB1727 ...
The Core binding factor (CBF) is a group of heterodimeric transcription factors. Core binding factors are composed of: a non- ... DNA-binding CBFβ chain (CBFB) a DNA-binding CBFα chain (RUNX1, RUNX2, RUNX3) de Bruijn M, Speck N (2004). "Core-binding factors ... Core Binding Factors at the US National Library of Medicine Medical Subject Headings (MeSH) AI-10-49. ...
... core-binding factors (CBFs). CBFs consist of a DNA-binding CBFα subunit and a non-DNA-binding CBFβ subunit. The t(8;21), ... Core-Binding Factor: A Central Player in Hematopoiesis and Leukemia. Nancy A. Speck, Terry Stacy, Qing Wang, Trista North, Ting ... Core-Binding Factor: A Central Player in Hematopoiesis and Leukemia. Nancy A. Speck, Terry Stacy, Qing Wang, Trista North, Ting ... Core-Binding Factor: A Central Player in Hematopoiesis and Leukemia. Nancy A. Speck, Terry Stacy, Qing Wang, Trista North, Ting ...
Core-binding factor acute myeloid leukemia (CBF-AML) data in Asian countries has been rarely reported. We analyzed 392 patients ... Prognostic factors and outcome of core binding factor acute myeloid leukemia patients with t(8;21) differ from those of ... Identification of molecular and cytogenetic risk factors for unfavorable core-binding factor-positive adult AML with post- ... Paschka P (2008) Core binding factor acute myeloid leukemia. Semin Oncol 35(4):410-417CrossRefGoogle Scholar ...
Eukaryotic transcriptional regulation is complex. Typically, it takes place by cooperative regulation. A number of studies have characterized this aspect i
... core binding factor plays a key role in several development pathways and in human disease; has been sequenced ... Transcription Factors: 20597*Core Binding Factors: 223*Core Binding Factor alpha Subunits: 18*core binding factor alpha: 16 ... Subscribe to New Research on core binding factor alpha core binding factor plays a key role in several development pathways and ... 11/07/2000 - "Binding of CBFalpha/AML/PEBP2alpha (core binding factor alpha/acute myelogenous leukemia/polyoma enhancer binding ...
Not all patients with core binding factor acute myeloid leukemia (CBF-AML) display a good outcome. Modern risk factors include ... Prospective evaluation of gene mutations and minimal residual disease in patients with core binding factor acute myeloid ... but MRD remained the sole prognostic factor in multivariate analysis. At 36 months, cumulative incidence of relapse and RFS ...
Expression of core-binding factor a1 and osteocalcin in fluoride-treated fibroblasts and osteoblasts. Author: Duan X, Xu H, ... which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in ... Tea Intake Is a Risk Factor for Skeletal Fluorosis. A number of recent studies have found that heavy tea drinkers can develop ...
Dasatinib (Sprycel™) in Patients With Newly Diagnosed Core Binding Factor (CBF) Acute Myeloid Leukemia (AML). The safety and ... Core binding factor (CBF) AML with molecular diagnosis of RUNX1-RUNX1T1 fusion transcript resulting from t(8;21)(q22;q22) (or a ... Genetics Home Reference related topics: Core binding factor acute myeloid leukemia Cytogenetically normal acute myeloid ... Adding dasatinib to intensive treatment in core-binding factor acute myeloid leukemia-results of the AMLSG 11-08 trial. ...
Description: Core binding factor A1 (CBFA1/RUNX2) is a runt-like transcription factor essential for osteoblast differentiation. ... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for ... osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors ...
Researchers Reveal Genomic Landscape of Core-Binding Factor Acute Myeloid Leukemia. By The ASCO Post. Posted: 11/4/2016 11:21: ... These genes encode for proteins that are part of the core-binding factor (CBF) complex, a transcriptional complex essential for ... University Pediatric Cancer Genome Project has completed a detailed map of the genomic landscape for core-binding factor acute ... the Pediatric Cancer Genome Project identified several mutations that may contribute to the development of core-binding factor ...
Prognostic factors and outcome of core binding factor acute myeloid leukemia patients with t(8;21) differ from those of ... For example, the fusion proteins AML1-ETO and core binding factor β (CBFβ)-MYH11 resulting from the t(8;21)(q22;q22) and (inv ... Histone Deacetylase Inhibitor Romidepsin Has Differential Activity in Core Binding Factor Acute Myeloid Leukemia. Olatoyosi M. ... Histone Deacetylase Inhibitor Romidepsin Has Differential Activity in Core Binding Factor Acute Myeloid Leukemia ...
Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor.. S Wang ... 4:233-242, 1990). We previously reported the purification of core-binding factors (CBF) from calf thymus nuclei (S. Wang and N. ... Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. ... Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. ...
Exon 8 splice site mutations in the gene encoding the E3-ligase CBL are associated with core binding factor acute myeloid ... CBL Exon 8/9 Mutants Activate the FLT3 Pathway and Cluster in Core Binding Factor/11q Deletion Acute Myeloid Leukemia/ ... All AML/MDS patients with identified CBL mutants belonged to the core binding factor and 11q deletion AML subtypes. ... Among 43 cases of core binding factor leukemias [t(8;21) and inv(16)] two patients expressing aberrant CBL transcripts were ...
Old and new prognostic factors in acute myeloid leukemia with deranged core-binding factor beta. Am J Hematol. 2013;88(7):594- ... Prognostic factors and outcome of core binding factor acute myeloid leukemia patients with t(8;21) differ from those of ... Identification of molecular and cytogenetic risk factors for unfavorable core-binding factor-positive adult AML with post- ... Core-binding factor acute myeloid leukemia: heterogeneity, monitoring and therapy. Am J Hematol. 2014;89(12):1121-1131. ...
RNA-sequencing Analysis of Core Binding Factor AML Identifies Recurrent ZBTB7A Mutations and Defines RUNX1-CBFA2T3 Fusion ... RNA-sequencing Analysis of Core Binding Factor AML Identifies Recurrent ZBTB7A Mutations and Defines RUNX1-CBFA2T3 Fusion ... RNA-sequencing Analysis of Core Binding Factor AML Identifies Recurrent ZBTB7A Mutations and Defines RUNX1-CBFA2T3 Fusion ... Targeting an RNA-Binding Protein Network in Acute Myeloid Leukemia. Wang E, Lu SX, Pastore A, Chen X, Imig J, Chun-Wei Lee S, ...
What is core-binding factor, runt domain, alpha subunit 3? Meaning of core-binding factor, runt domain, alpha subunit 3 medical ... What does core-binding factor, runt domain, alpha subunit 3 mean? ... core-binding factor, runt domain, alpha subunit 3 explanation ... Looking for online definition of core-binding factor, runt domain, alpha subunit 3 in the Medical Dictionary? ... Core-binding factor, runt domain, alpha subunit 3 , definition of core-binding factor, runt domain, alpha subunit 3 by Medical ...
Core binding factor a1 (CBFA1) is a key regulator of osteoblast differentiation. This study was designed to investigate the ... Functional analysis of core binding factor a1 and its relationship with related genes expressed by human periodontal ligament ... and receptor activator nuclear factor kappa B ligand (RANKL) were detected before and after RNA interference (RNAi) of CBFA1. ...
What is SL3/AKV core-binding factor alpha B subunit? Meaning of SL3/AKV core-binding factor alpha B subunit medical term. What ... SL3/AKV core-binding factor alpha B subunit explanation free. ... does SL3/AKV core-binding factor alpha B subunit mean? ... Looking for online definition of SL3/AKV core-binding factor alpha B subunit in the Medical Dictionary? ... SL3/AKV core-binding factor alpha B subunit , definition of SL3/AKV core-binding factor alpha B subunit by Medical dictionary ...
Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2, (10ug), 10 ... Home » cDNA » Mouse cDNA » Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 ( ... MC219266 Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), ... Properties for Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) ( ...
Rna Polymerase Ii Core Promoter Proximal Region Sequence-Specific Dna Binding Transcription Factor Activity Involved In ... Rna Polymerase Ii Core Promoter Proximal Region Sequence-Specific Dna Binding Transcription Factor Activity Involved In ... Rna Polymerase Ii Core Promoter Proximal Region Sequence-Specific Dna Binding Transcription Factor Activity Involved In ... RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in ...
Acute MyeloidGeneSubunitsEukaryoticInitiationAcuteSubunit betaBeta subunitBiolRepressorPMIDRUNX1LigandTAFsChromatinVitroSequence-specificAlpha subunitHeterodimeric transcription factorsPromoter elementUpstreamPreinitiation complexPathwaysActivatesConsensus sequenceProteins bindTATA-Box BinTranscriptional activationMutationsEnhancers2001SiteEpidermal Growth
- Core Binding Factor (CBF) Acute Myeloid Leukemia (AML) is cytogenetically characterized by either the t(8;21) or the inv(16)/t(16;16) chromosomal abnormalities, which, although being pathognomonic, are not sufficient per se to induce overt leukemia but rather determine a preclinical phase of disease when preleukemic subclones compete until the acquisition of clonal dominance by one of them. (hindawi.com)
- The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g. (nih.gov)
- D816V mutation in the KIT gene activation loop has greater cell-proliferative and anti-apoptotic ability than N822K mutation in core-binding factor acute myeloid leukemia. (nih.gov)
- The t(8;21), associated with de novo acute myeloid leukemias, disrupts the CBFA2 ( AML1 ) gene, which encodes a DNA-binding CBF α subunit. (aacrjournals.org)
- The CBFB gene, which encodes the non-DNA-binding subunit of the CBFs, is disrupted by the inv(16) in de novo acute myeloid leukemias. (aacrjournals.org)
- A common chromosomal translocation in acute myeloid leukemia (AML) involves the AML1 (acute myeloid leukemia 1, also called RUNX1, core binding factor protein (CBF alpha), and PEBP2 alpha B) gene on chromosome 21 and the ETO (eight-twenty one, also called MTG8) gene on chromosome 8. (curehunter.com)
- Prospective evaluation of gene mutations and minimal residual disease in patients with core binding factor acute myeloid leukemia. (nih.gov)
- Modern risk factors include KIT and/or FLT3 gene mutations and minimal residual disease (MRD) levels, but their respective values have never been prospectively assessed. (nih.gov)
- Higher WBC, KIT, and/or FLT3-ITD/TKD gene mutations, and a less than 3-log MRD reduction after first consolidation, were associated with a higher specific hazard of relapse, but MRD remained the sole prognostic factor in multivariate analysis. (nih.gov)
- It is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. (ihcworld.com)
- A gene on chromosome 1p36 that encodes a member of the runt domain-containing family of transcription factors. (thefreedictionary.com)
- A gene on chromosome 21q22.3 that encodes the alpha subunit of core binding factor (CBF), a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. (thefreedictionary.com)
- 2002). Chu G: p53 Binds and activates the xeroderma pigmentosum DDB2 gene in humans but not mice. (core.ac.uk)
- CBFB (Core-Binding Factor Beta Subunit) is a Protein Coding gene. (genecards.org)
- GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and transcription coactivator activity . (genecards.org)
- The CBFB gene provides instructions for making a protein called core binding factor beta (CBFβ), which is one piece of a protein complex known as core binding factor (CBF). (nih.gov)
- The protein produced from the fusion gene, called CBFβ-MYH11, can still bind to RUNX1 to form CBF. (nih.gov)
- The presence of CBFβ-MYH11 may block binding of CBF to DNA, preventing RUNX1 from controlling gene activity. (nih.gov)
- The protein produced from the FOXF1 gene is a transcription factor, which means that it attaches (binds) to specific regions of DNA and helps control the activity of many other genes. (nih.gov)
- The protein produced from the normal RUNX1 gene is part of a protein complex known as core binding factor (CBF). (nih.gov)
- The normal RUNX1 protein, produced from the RUNX1 gene, is part of a protein complex called core binding factor (CBF) that attaches (binds) to DNA and turns on genes involved in blood cell development. (nih.gov)
- A yeast mutant was isolated encoding a single amino acid substitution [serine-53 → proline (S53P)] in transcription factor TFIIB that impairs activation of the PHO5 gene in response to phosphate starvation. (pnas.org)
- Transcriptional activators stimulate gene expression by binding enhancer elements and contacting, either directly or indirectly, components of the RNA polymerase II (RNAPII) transcriptional machinery. (pnas.org)
- BACKGROUND: Transcription factors (TFs) and their binding sites (TFBSs) play a central role in the regulation of gene expression. (biomedsearch.com)
- of the fusion protein prevents binding to DNA CBF, affecting its ability to control gene activity. (ivami.com)
- Next-generation sequencing with a myeloid gene panel in core-binding factor AML showed KIT activation loop and TET2 mutations predictive of outcome. (cdc.gov)
- The two are connected by gene expression, and it is generally thought that variation in transcription factor ( TF ) function is an important determinant of phenotypic evolution. (plantcell.org)
- Gene expression complexity is positively correlated with the total number of bound TFs , revealing insights in the regulatory code for genes with different expression breadths. (plantcell.org)
- Possible explanations are the dependency on other condition-specific factors, such as cofactors or chromatin remodeling, for the correct regulation of the target gene, or that many of the observed binding events are nonfunctional. (plantcell.org)
- Cooperative assemblies of transcription factors (TFs) on target gene enhancers coordinate cell proliferation, fate specification, and differentiation through precise and complicated transcriptional mechanisms. (rcsb.org)
- Nuclear factor of activated T-cells, cytoplasmic 1 is a protein that in humans is encoded by the NFATC1 gene . (wikipedia.org)
- The product of this gene is a component of the nuclear factor of activated T cells DNA-binding transcription complex. (wikipedia.org)
- Proteins belonging to this family of transcription factors play a central role in inducible gene transcription during immune response. (wikipedia.org)
- The binding of transcription factor proteins (TFs) to DNA promoter regions upstream of gene transcription start sites (TSSs) is one of the most important mechanisms by which gene expression, and thus many cellular processes, are controlled. (bioconductor.org)
- Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. (wikipedia.org)
- Core binding factor acute myeloid leukaemia is a cancer related to genetic changes in the CBF gene. (wikipedia.org)
- Runt-related transcription factor 1 (RUNX1) also known as acute myeloid leukemia 1 protein (AML1) or core-binding factor subunit alpha-2 (CBFA2) is a protein that in humans is encoded by the RUNX1 gene. (wikipedia.org)
- The transcription of RUNX1 is regulated by 2 enhancers (regulatory element 1 and regulatory element 2), and these tissue specific enhancers enable the binding of lymphoid or erythroid regulatory proteins, therefore the gene activity of RUNX1 is highly active in the haematopoietic system. (wikipedia.org)
- The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. (wikipedia.org)
- HMGB1 is an intracellular protein that can translocate to the nucleus where it binds DNA and regulates gene expression. (wikipedia.org)
- Perlecan (PLC) also known as basement membrane-specific heparan sulfate proteoglycan core protein (HSPG) or heparan sulfate proteoglycan 2 (HSPG2), is a protein that in humans is encoded by the HSPG2 gene. (wikipedia.org)
- Fibroblast growth factor-binding protein 1 is a protein that in humans is encoded by the FGFBP1 gene. (wikipedia.org)
- In the process of transcription (by any polymerase), there are three main stages: Initiation: the construction of the RNA polymerase complex on the gene's promoter with the help of transcription factors Elongation: the actual transcription of the majority of the gene into a corresponding RNA sequence Termination: the cessation of RNA transcription and the disassembly of the RNA polymerase complex. (wikipedia.org)
- As Pol I escapes and clears the promoter, UBF and SL1 remain-promoter bound, ready to recruit another Pol I. Indeed, each active rDNA gene can be transcribed multiple times simultaneously, as opposed to Pol II-transcribed genes, which associate with only one complex at a time. (wikipedia.org)
- RNA-binding protein 39 is a protein that in humans is encoded by the RBM39 gene. (wikipedia.org)
- The protein encoded by this gene is an RNA binding protein and possible splicing factor. (wikipedia.org)
- CREB-binding protein, also known as CREBBP or CBP, is a protein that in humans is encoded by the CREBBP gene. (wikipedia.org)
- This gene is ubiquitously expressed and is involved in the transcriptional coactivation of many different transcription factors. (wikipedia.org)
- First isolated as a nuclear protein that binds to cAMP-response element-binding protein (CREB), this gene is now known to play critical roles in embryonic development, growth control, and homeostasis by coupling chromatin remodeling to transcription factor recognition. (wikipedia.org)
- Transcription initiation factor TFIID subunit 11 also known as TAFII28, is a protein that in humans is encoded by the TAF11 gene. (wikipedia.org)
- The protein encoded by this gene belongs to the ETS family of transcription factors. (wikipedia.org)
- These transcription factors have specific activator or repressor sequences of corresponding nucleotides that attach to specific promoters and regulate gene expression. (wikipedia.org)
- The promoter is located at the 5' end of the gene and is composed of a core promoter sequence and a proximal promoter sequence. (wikipedia.org)
- If a transcription factor binds to an enhancer in a 5' flanking region, the DNA strand bends in a way that the transcription factor that is bound to the enhancer can also bind the promoter of a gene. (wikipedia.org)
- Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. (wikipedia.org)
- CCCTC-Binding factor or CTCF was initially discovered as a negative regulator of the chicken c-myc gene. (wikipedia.org)
- CTCF binding has also been both shown to promote and repress gene expression. (wikipedia.org)
- The protein CTCF plays a heavy role in repressing the insulin-like growth factor 2 gene, by binding to the H-19 imprinting control region (ICR) along with differentially-methylated region-1 (DMR1) and MAR3. (wikipedia.org)
- The human genome contains anywhere between 15,000-40,000 CTCF binding sites depending on cell type, suggesting a widespread role for CTCF in gene regulation. (wikipedia.org)
- Because heterodimerization is a prerequisite for DNA binding, factors that interfere with dimerization effectively act as passive repressors of proneural gene activity. (wikipedia.org)
- TP53 gene encodes proteins that bind to DNA and regulate gene expression to prevent mutations of the genome. (wikipedia.org)
- Gene transcription by RNA polymerase II depends on the regulation of the core promoter by long-range regulatory elements such as enhancers and silencers. (wikipedia.org)
- The type of core promoter affects the level of transcription and expression of a gene. (wikipedia.org)
- Keratinocyte growth factor is a protein that in humans is encoded by the FGF7 gene. (wikipedia.org)
- The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. (wikipedia.org)
- Transcription initiation factor TFIID subunit 1, also known as transcription initiation factor TFIID 250 kDa subunit (TAFII-250) or TBP-associated factor 250 kDa (p250), is a protein that in humans is encoded by the TAF1 gene. (wikipedia.org)
- Full gene expression occurs when transcription activator proteins bind to each module within the regulatory promoter. (wikipedia.org)
- The CAAT Box is what is known as a core promoter, also known as the basal promoter or simply the promoter, is a region of DNA that initiates transcription of a particular gene. (wikipedia.org)
- This region, in particular for the CAAT Box, is located about 60-100 bases upstream (towards the 5' end), however no less than 27 base pairs away, from the initial transcription site or a eukaryote gene in which a complex of general transcription factors bind with RNA Polymerase II prior to the initiation of transcription. (wikipedia.org)
- Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. (wikipedia.org)
- This gene encodes the GA-binding protein transcription factor, beta subunit. (wikipedia.org)
- Other proteins such as coactivators, chromatin remodelers, histone acetyltransferases, histone deacetylases, kinases, and methylases are also essential to gene regulation, but lack DNA-binding domains, and therefore are not TFs. (wikipedia.org)
- Transcription factors are essential for the regulation of gene expression and are, as a consequence, found in all living organisms. (wikipedia.org)
- The number of transcription factors found within an organism increases with genome size, and larger genomes tend to have more transcription factors per gene. (wikipedia.org)
- Hence, the combinatorial use of a subset of the approximately 2000 human transcription factors easily accounts for the unique regulation of each gene in the human genome during development. (wikipedia.org)
- Depending on the transcription factor, the transcription of the adjacent gene is either up- or down-regulated. (wikipedia.org)
- Transcription factors use a variety of mechanisms for the regulation of gene expression. (wikipedia.org)
- They bind to the DNA and help initiate a program of increased or decreased gene transcription. (wikipedia.org)
- The preinitiation complex binds to promoter regions of DNA upstream to the gene that they regulate. (wikipedia.org)
- First, an RNA polymerase along with general transcription factors binds to the promoter region of the gene to form a closed complex called the preinitiation complex. (wikipedia.org)
- As one of the few proteins in the preinitiation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. (wikipedia.org)
- Binding of TFIID to the TATA box in the promoter region of the gene initiates the recruitment of other factors required for RNA Pol II to begin transcription. (wikipedia.org)
- Transcription factors bind to hypersensitive site cores and cause the LCR to form a loop that can interact with the promoter of the gene it regulates. (wikipedia.org)
- The complex moves along the DNA helix until it can bind to the promoter of the gene it regulates. (wikipedia.org)
- Once bound, the transcriptional apparatus increases gene expression. (wikipedia.org)
- This hypothesis combines the looping and tracking models, suggesting that the transcription factors bind to the LCR to form a loop, which then seeks and binds to the promoter of the gene it regulates. (wikipedia.org)
- Transcription initiation factor TFIID subunit 8 is a protein that in humans is encoded by the TAF8 gene. (wikipedia.org)
- This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. (wikipedia.org)
- Runt-related transcription factor 3 is a protein that in humans is encoded by the RUNX3 gene. (wikipedia.org)
- CREs typically regulate gene transcription by binding to transcription factors. (wikipedia.org)
- In order to initiate transcription of the downstream gene, a host of DNA-binding proteins called transcription factors (TFs) must bind sequentially to this region. (wikipedia.org)
- Only once this region has been bound with the appropriate set of TFs, and in the proper order, can RNA polymerase bind and begin transcribing the gene. (wikipedia.org)
- In contrast, trans-regulatory elements are diffusible factors, usually proteins, that may modify the expression of genes distant from the gene that was originally transcribed to create them. (wikipedia.org)
- For example, a transcription factor that regulates a gene on chromosome 6 might itself have been transcribed from a gene on chromosome 11. (wikipedia.org)
- DNA TATA box Pribnow box SOS box CAAT box CCAAT box Operator (biology) Upstream activation sequence RNA List of cis-regulatory RNA elements Polyadenylation signals, mRNA AU-rich element, mRNA Other Regulation of gene expression cis-regulatory module Gene regulatory network Operon Promoter Trans-acting factor Rfam Transterm Butler JE, Kadonaga JT (October 2002). (wikipedia.org)
- This gene regulation is in large part controlled, in a tissue-specific manner, by the binding of transcription factors to noncoding genomic regions referred to as cis-regulatory modules (CRMs), activating or repressing gene expression by modulating the structure of the chromatin and therefore having a positive or negative effect on transcription regulation. (wikipedia.org)
- Transcription initiation factor TFIID subunit 9B is a protein that in humans is encoded by the TAF9B gene. (wikipedia.org)
- This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID. (wikipedia.org)
- Megf8 also known as Multiple Epidermal Growth Factor-like Domains 8, is a protein coding gene that encodes a single pass membrane protein, known to participate in developmental regulation and cellular communication. (wikipedia.org)
- Transcription initiation factor TFIID subunit 13 is a protein that in humans is encoded by the TAF13 gene. (wikipedia.org)
- Transcription initiation factor TFIID subunit 5 is a protein that in humans is encoded by the TAF5 gene. (wikipedia.org)
- Most importantly is the idea of combinatorial control, which is that any given gene is likely controlled by a specific combination of factors to control transcription. (wikipedia.org)
- Prokaryotic transcription is governed by three main sequence elements: Promoters are elements of DNA that may bind RNA polymerase and other proteins for the successful initiation of transcription directly upstream of the gene. (wikipedia.org)
- Operators recognize repressor proteins that bind to a stretch of DNA and inhibit the transcription of the gene. (wikipedia.org)
- This strategy of control is distinct from eukaryotic transcription, whose basal state is to be off and where co-factors required for transcription initiation are highly gene dependent. (wikipedia.org)
- TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L is an enzyme that in humans is encoded by the TAF6L gene. (wikipedia.org)
- Transcription initiation factor TFIID subunit 4 is a protein that in humans is encoded by the TAF4 gene. (wikipedia.org)
- TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa, also known as TAF9, is a protein that in humans is encoded by the TAF9 gene. (wikipedia.org)
- This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16. (wikipedia.org)
- Transcription initiation factor TFIID subunit 6 is a protein that in humans is encoded by the TAF6 gene. (wikipedia.org)
- This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. (wikipedia.org)
- TATA box-binding protein-like protein 1 is a protein that in humans is encoded by the TBPL1 gene. (wikipedia.org)
- TATA-binding protein-associated factor 2N is a protein that in humans is encoded by the TAF15 gene. (wikipedia.org)
- Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. (wikipedia.org)
- Although such arrays are perfectly suited to study gene expression profiles, they have limited importance in ChIP experiments since most "interesting" proteins with respect to this technique bind in intergenic regions. (wikipedia.org)
- TAF function== There are several functions of TAF in its role as part of the TFIID complex, their function is to interact with the following * specific transcriptional activators, * basal transcription factors, * other TAFIIs, * specific DNA sequences, for example the downstream promoter element or gene-specific core promoter sequence. (wikipedia.org)
- Transcription initiation factor TFIID subunit 4B is a protein that in humans is encoded by the TAF4B gene. (wikipedia.org)
- It is a bacterial transcription initiation factor that enables specific binding of RNA polymerase to gene promoters. (wikipedia.org)
- The specific sigma factor used to initiate transcription of a given gene will vary, depending on the gene and on the environmental signals needed to initiate transcription of that gene. (wikipedia.org)
- σ19 (FecI) - the ferric citrate sigma factor, regulates the fec gene for iron transport σ24 (RpoE) - the extracytoplasmic/extreme heat stress sigma factor σ28 (RpoF) - the flagellar sigma factor σ32 (RpoH) - the heat shock sigma factor, it is turned on when the bacteria are exposed to heat. (wikipedia.org)
- Krüppel-like factor 15 is a protein that in humans is encoded by the KLF15 gene in the Krüppel-like factor family. (wikipedia.org)
- Using deletion and mutation analysis, EMSA and ChIP, demonstrated that USF1 and Spl can bind to E-box in-80 to-45 and GC-box in-189 to-155 in the KLF15 promoter respectively, thus regulating the transcription of KLF15 gene. (wikipedia.org)
- Before the start of transcription, the transcription Factor II D (TFIID) complex binds to the TATA box in the core promoter of the gene. (wikipedia.org)
- TFIID and B-TFIID are not equivalent, since transcription reactions utilizing TFIID are responsive to gene specific transcription factors such as SP1, while reactions reconstituted with B-TFIID are not. (wikipedia.org)
- APOBEC1 complementation factor is a protein that in humans is encoded by the A1CF gene. (wikipedia.org)
- Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC1 and a complementation factor encoded by this gene. (wikipedia.org)
- The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. (wikipedia.org)
- Tissue factor, also called platelet tissue factor, factor III, or CD142 is a protein encoded by the F3 gene present in subendothelial tissue and leukocytes. (wikipedia.org)
- The F3 gene encodes coagulation factor III which is a cell surface glycoprotein. (wikipedia.org)
- Neutrophil cytosol factor 4 is a protein that in humans is encoded by the NCF4 gene. (wikipedia.org)
- Histone acetylation and deacetylation are the processes by which the lysine residues within the N-terminal tail protruding from the histone core of the nucleosome are acetylated and deacetylated as part of gene regulation. (wikipedia.org)
- Krueppel-like factor 6 is a protein that in humans is encoded by the KLF6 gene. (wikipedia.org)
- Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2), or prolyl hydroxylase domain-containing protein 2 (PHD2), is an enzyme encoded by the EGLN1 gene. (wikipedia.org)
- These gene products may include proteins such as glycolytic enzymes and angiogenic growth factors. (wikipedia.org)
- It occurs due to haploinsufficiency caused by mutations in the CBFA1 gene (also called Runx2), located on the short arm of chromosome 6, which encodes transcription factor required for osteoblast differentiation. (wikipedia.org)
- Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that in humans is encoded by the RUNX2 gene. (wikipedia.org)
- The gene is highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit gene. (wikipedia.org)
- In addition to the highly conserved Runt domain, the AML-1 gene product carries a putative ATP-binding site (GRSGRGKS), and has a C-terminal region rich in proline and serine residues. (wikipedia.org)
- This gene encodes a member of the calcium-dependent activator of secretion (CAPS) protein family, which are calcium-binding proteins that regulate the exocytosis of synaptic and dense-core vesicles in neurons and neuroendocrine cells. (wikipedia.org)
- This gene interacts with brain-derived neurotrophic factor. (wikipedia.org)
- TATA box-binding protein-associated factor RNA polymerase I subunit B is an enzyme that in humans is encoded by the TAF1B gene. (wikipedia.org)
- Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. (asm.org)
- However, CBF beta p22.0 and CBF beta p21.5 form a complex with DNA-binding CBF alpha subunits and as a result decrease the rate of dissociation of the CBF protein-DNA complex. (asm.org)
- Association of the CBF beta subunits does not extend the phosphate contacts in the binding site. (asm.org)
- Purification and cDNA cloning revealed that PEBP2 has two subunits, DNA-binding alpha (PEBP2alpha) and non-DNA-binding beta (PEBP2beta). (nih.gov)
- The N-terminal 141 amino acids of CBFß contains the heterodimerization domain for the DNA-binding CBFα subunits and binds to CBFα in vitro with the same affinity as the full length CBFß . (dartmouth.edu)
- CBF binds to DNA as a heterodimer of the α and β subunits. (jimmunol.org)
- All the α subunits contain a highly conserved runt domain that is responsible for both DNA binding and dimerization with the CBFβ subunit ( 15 , 16 , 17 , 18 , 19 , 20 ), which by itself cannot bind DNA but increases the affinity of CBFα protein for DNA about fivefold ( 16 , 17 , 18 ). (jimmunol.org)
- In fact, the RUNX family is often referred to as α-subunits, together with binding of a common β-subunit CBFβ, RUNX can behave as heterodimeric transcription factors collectively called the core binding factors (CBFs). (wikipedia.org)
- The ligand-binding cavities are located at the interface between the subunits. (wikipedia.org)
- The other two subunits are related to Pol II initiation factors and have structural homologues in Pol III. (wikipedia.org)
- The crystal structure of hTAFII28 with hTAFII18 shows that this region is involved in the binding of these two subunits. (wikipedia.org)
- In humans, eIF3 consists of 13 nonidentical subunits (eIF3a-m) with a combined molecular weight of ~800 kDa, making it the largest translation initiation factor. (wikipedia.org)
- Several subunits of eIF3 contain RNA recognition motifs (RRMs) and other RNA binding domains to form a multisubunit RNA binding interface through which eIF3 interacts with cellular and viral IRES mRNA, including the HCV IRES. (wikipedia.org)
- All five core subunits of budding yeast's eIF3 are present in heat-induced stress granules, along with several other translation factors. (wikipedia.org)
- The eIF3 subunits exist at equal stoichiometry within the complex, with the exception of eIF3J, which is loosely bound and non-essential for viability in several species. (wikipedia.org)
- All three eukaryotic polymerases have five core subunits that exhibit homology with the β, β', αI, αII, and ω subunits of E. coli RNA polymerase. (wikipedia.org)
- The additional subunits found in Pol I and Pol III relative to Pol II, are homologous to Pol II transcription factors. (wikipedia.org)
- Each of these transcription factors is formed from the interaction of many protein subunits, indicating that transcription is a heavily regulated process. (wikipedia.org)
- In vertebrates, Complex III contains 11 subunits: 3 respiratory subunits, 2 core proteins and 6 low-molecular weight proteins. (wikipedia.org)
- This subunit interacts strongly with two TFIID subunits that show similarity to histones H3 and H4, and it may participate in forming a nucleosome-like core in the TFIID complex. (wikipedia.org)
- One of the isoforms has been shown to preclude binding of one of the other TFIID subunits, thereby reducing transcription and initiating signals that trigger apoptosis. (wikipedia.org)
- Core-binding factor (CBF) is a heterodimeric transcription factor composed by the CBFβ and RUNX subunits (the latter is encoded by RUNX1, RUNX2, or RUNX3 genes). (wikipedia.org)
- The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. (wikipedia.org)
- Coordinates the activities of more than 70 polypeptides required for initiation of transcription by RNA polymerase II Binds to the core promoter to position the polymerase properly Serves as the scaffold for assembly of the remainder of the transcription complex Acts as a channel for regulatory signals TFIID is itself composed of TBP and several subunits called TATA-binding protein Associated Factors (TBP-associated factors, or TAFs). (wikipedia.org)
- These histone cores are composed of 8 subunits, two each of H2A, H2B, H3 and H4 histones. (wikipedia.org)
- These structures reveal the precise architecture of eukaryote-specific elements, their interaction with the universally conserved core, and all eukaryote-specific bridges between the two ribosomal subunits. (wikipedia.org)
- The eukaryotic translation initiation factor (eIF) 4B promotes the RNA-dependent ATP hydrolysis activity and ATP-dependent RNA helicase activity of eIF4A and eIF4F during translation initiation. (core.ac.uk)
- SRF-like/Type I subfamily of MADS (MCM1, Agamous, Deficiens, and SRF (serum response factor)) box family of eukaryotic transcriptional regulators [ PMID: 7744019 ]. (ebi.ac.uk)
- The dimeric eukaryotic upstream binding factor (UBF) binds the UCE and the core element. (wikipedia.org)
- Other promoter elements found in eukaryotic 5' flanking regions include initiator elements, downstream core promoter element, CAAT box, and the GC box. (wikipedia.org)
- The TATA box was the first eukaryotic core promoter motif to be identified in 1978 by American biochemist David Hogness while he and his graduate student, Michael Goldberg where on sabbatical at the University of Basel in Switzerland. (wikipedia.org)
- Eukaryotic initiation factor 3 (eIF3) is a multiprotein complex that functions during the initiation phase of eukaryotic translation. (wikipedia.org)
- 9aaTAD is a novel domain common to a large superfamily of eukaryotic transcription factors represented by Gal4, Oaf1, Leu3, Rtg3, Pho4, Gln4, Gcn4 in yeast and by p53, NFAT, NF-κB and VP16 in mammals. (wikipedia.org)
- It is homologous to archaeal transcription factor B and to eukaryotic TFIIB. (wikipedia.org)
- The Rel homology domain (RHD) is a protein domain found in a family of eukaryotic transcription factors, which includes NF-κB, NFAT, among others. (wikipedia.org)
- This is impossible in eukaryotes, where transcription occurs in a membrane-bound nucleus while translation occurs outside the nucleus in the cytoplasm (see also Eukaryotic transcription). (wikipedia.org)
- Compared to their prokaryotic homologs, many of the eukaryotic ribosomal proteins are enlarged by insertions or extensions to the conserved core. (wikipedia.org)
- Atomic coordinates (PDB files) and structure factors of the eukaryotic ribosome have been deposited in the Protein Data Bank (PDB) under the following accession codes: Some general architectural features of the ribosome are conserved across kingdoms: The shape of the small subunit can be subdivided into two large segments, the head and the body. (wikipedia.org)
- Processing of core histones is done differently because typical histone mRNA lacks several features of other eukaryotic mRNAs, such as poly(A) tail and introns. (wikipedia.org)
- Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. (uniprot.org)
- Although the function of eIF4B is conserved among plants, animals, and yeast, eIF4B is one of the least conserved of initiation factors at the sequence level. (core.ac.uk)
- Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. (uniprot.org)
- TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. (wikipedia.org)
- The binding of different transcription factors, therefore, regulates the rate of transcription initiation at different times and in different cells. (wikipedia.org)
- Binding of activators and repressors to multiple regulatory sequences has a cooperative effect on transcription initiation. (wikipedia.org)
- It also binds to activators and other transcriptional regulators, and these interactions affect the rate of transcription initiation. (wikipedia.org)
- eIF3 binds the small ribosomal subunit (40S) at and near its solvent side and serves as a scaffold for several other initiation factors, the auxiliary factor DHX29, and mRNA. (wikipedia.org)
- This region modulates the DNA binding activity of the C-terminus, and modulation of DNA-binding affects the rate of transcription complex formation and initiation of transcription. (wikipedia.org)
- TBP associates with a host of factors, including the general transcription factors TFIIA, -B, -D, -E, and -H, to form huge multi-subunit pre-initiation complexes on the core promoter. (wikipedia.org)
- The complete enzyme with σ is termed the RNA polymerase holoenzyme and is necessary for correct initiation of transcription, whereas the core enzyme can continue transcription after initiation. (wikipedia.org)
- TAF10 has been shown to interact with TAF9, Transcription initiation protein SPT3 homolog, TAF13 and TATA binding protein. (wikipedia.org)
- Sigma factors are specialized bacterial proteins that bind to RNA polymerases and orchestrate transcription initiation. (wikipedia.org)
- A sigma factor (σ factor) is a protein needed only for initiation of transcription. (wikipedia.org)
- Once initiation of RNA transcription is complete, the sigma factor can leave the complex. (wikipedia.org)
- All studies are consistent with the assumption that promoter escape reduces the lifetime of the sigma-core interaction from very long at initiation (too long to be measured in a typical biochemical experiment) to a shorter, measurable lifetime upon transition to elongation. (wikipedia.org)
- Transcription of polymerase I starts with a Pol I initiation complex that binds to the rDNA promoter. (wikipedia.org)
- The formation of this complex requires the help of an upstream activating factor or UAF that associates with TATA-box binding protein and the core factor (CF). Together the two transcription factors allow the RNA pol I complex to bind with the polymerase I initiation factor, Rrn3. (wikipedia.org)
- Additional transcription regulation comes from transcription factors that can affect the stability of the holoenzyme structure at initiation. (wikipedia.org)
- Core Binding Factor (CBF) Acute Myeloid Leukemia (AML) is cytogenetically characterized by either the t(8;21) or the inv(16)/t(16;16) chromosomal abnormalities, which, although being pathognomonic, are not sufficient per se to induce overt leukemia but rather determine a preclinical phase of disease when preleukemic subclones compete until the acquisition of clonal dominance by one of them. (hindawi.com)
- Core-binding factor acute myeloid leukemia (CBF-AML) data in Asian countries has been rarely reported. (springer.com)
- Not all patients with core binding factor acute myeloid leukemia (CBF-AML) display a good outcome. (nih.gov)
- Researchers in the Pediatric Cancer Genome Project identified several mutations that may contribute to the development of core-binding factor acute myeloid leukemia as cooperating mutations, including changes in CCND2 , and new recurrent mutations, including alterations in DHX15 . (ascopost.com)
- An international team of researchers from the St. Jude Children's Research Hospital-Washington University Pediatric Cancer Genome Project has completed a detailed map of the genomic landscape for core-binding factor acute myeloid leukemia (CBF-AML). (ascopost.com)
- Core binding factor acute myeloid leukemia (AML) comprises two subtypes with distinct cytogenetic abnormalities of either t(8;21)(q22;q22) or inv(16)(p13q22)/t(16;16)(p13;q22). (haematologica.org)
- Core binding factor (CBF) leukemia represents up to 12% of all newly diagnosed adult acute myeloid leukemia (AML). (haematologica.org)
- Study of phase II, multicenter, open, assessing dasatinib in patients of acute myelogenous leukemia (AML) to core binding factors (CBF) resistant to conventional chemotherapy or molecular relapse. (springer.com)
- Comprehensive mutational profiling of core binding factor acute myeloid leukemia. (cdc.gov)
- Acute myeloid leukemia (AML) with t(8;21) or inv(16) have been recognized as unique entities within AML and are usually reported together as core binding factor AML (CBF-AML). (cdc.gov)
- To address these issues, we performed extensive mutational analysis by high-throughput sequencing in 215 patients with CBF-AML enrolled in the Phase 3 Trial of Systematic Versus Response-adapted Timed-Sequential Induction in Patients With Core Binding Factor Acute Myeloid Leukemia and Treating Patients with Childhood Acute Myeloid Leukemia with Interleukin-2 trials (age, 1-60 years). (cdc.gov)
- SNP-array lesions in core binding factor acute myeloid leukemia. (cdc.gov)
- Detection of KIT mutations in core binding factor acute myeloid leukemia. (cdc.gov)
- A team of investigators led by scientists at St. Jude Children's Research Hospital and the Washington University Pediatric Cancer Genome Project (PCGP) has published a comprehensive map of the genomic landscape for the form of acute myeloid leukemia (AML) called core-binding factor acute myeloid leukemia (CBF-AML). (clinicalomics.com)
- This new work reveals differences in mutations that contribute to the diversity of CBF-AML-the findings of which were published recently in Nature Genetics through an article entitled " The genomic landscape of core-binding factor acute myeloid leukemias . (clinicalomics.com)
- A genetic rearrangement (translocation) involving chromosome 21 is associated with a type of blood cancer known as core binding factor acute myeloid leukemia (CBF-AML). (nih.gov)
- Characterization of mutational pattern of patients with core-binding factor acute myeloid leukemia]. (cdc.gov)
- To characterize the mutational profile of patients with core-binding factor acute myeloid leukemia (CBF-AML). (cdc.gov)
- Prognostic Importance of C-KIT Mutations in Core Binding Factor Acute Myeloid Leukemia: A Systematic Review. (cdc.gov)
- Activating mutations in RAS and receptor tyrosine kinases such as KIT and FLT3 are hypothesized to cooperate with chimeric transcription factors in the pathogenesis of acute myeloid leukemia (AML). (elsevier.com)
- Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. (unibo.it)
- In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. (unibo.it)
- Acute myeloid leukemia factor nuclear binding (CBF-LMA) is a form of neoplasia marrow called acute myelogenous leukemia. (ivami.com)
- Clinical outcome and mutations of 96 core-binding factor acute myeloid leukemia (AML) patients 18-60 years old were examined. (cdc.gov)
- CBFα is also known as acute myeloid leukemia (AML) or polyomavirus enhancer core-binding protein-2α (PEBP2α). (jimmunol.org)
- What Are the Risk Factors for Acute Myeloid Leukemia? (medlineplus.gov)
- Later on, the term "activator sites" was opposed to "operator sites", where operator sites were limited to sites for the binding of repressor regulators. (wikipedia.org)
- For example, some repressor proteins can bind to the core promoter to prevent polymerase binding. (wikipedia.org)
- This region is responsible for binding the p53 co-repressor LMO3. (wikipedia.org)
- Alternatively a repressor and polymerase may bind to the DNA at the same time with a physical interaction between the repressor preventing the opening of the DNA for access to the minus strand for transcription. (wikipedia.org)
- They act by binding activator or repressor proteins (transcription factors) and the intervening DNA bends such that the bound proteins contact the core promoter and RNA polymerase. (wikipedia.org)
- Core binding factors are composed of: a non-DNA-binding CBFβ chain (CBFB) a DNA-binding CBFα chain (RUNX1, RUNX2, RUNX3) de Bruijn M, Speck N (2004). (wikipedia.org)
- CBFB enhances DNA binding by RUNX1. (nih.gov)
- uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a fluorescence resonance energy transfer (FRET) assay. (nih.gov)
- Confirmation of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a time resolved fluorescence resonance energy transfer (TR-FRET) assay. (nih.gov)
- The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. (grantome.com)
- runt-related transcription factor 1 (RUNX1) In vitro and mouse studies suggest inhibiting. (biocentury.com)
- CBFβ attaches (binds) to one of three related RUNX proteins (RUNX1, RUNX2, or RUNX3) to form different versions of CBF. (nih.gov)
- and RUNX1) of a protein complex known as core binding factor (CBF). (ivami.com)
- RUNX1 is a transcription factor that regulates the differentiation of hematopoietic stem cells into mature blood cells. (wikipedia.org)
- These domains are necessary for RUNX1 to mediate DNA binding and protein-protein interactions respectively. (wikipedia.org)
- The runt domain of RUNX1 binds to the core consensus sequence TGTGGNNN (where NNN can represent either TTT or TCA). (wikipedia.org)
- RUNX1 can bind DNA as a monomer, but its DNA binding affinity is enhanced by 10 fold if it heterodimerises with the core binding factor β (CBFβ), also via the runt domain. (wikipedia.org)
- AI-10-49 allosterically binds to CBFβ-SMMHC and disrupts protein-protein interaction between CBFβ-SMMHC and tumor suppressor RUNX1. (wikipedia.org)
- Current treatment for inv(16) AML uses chemotherapy drugs, such as doxorubicin and cytarabine, with an estimated 5-year overall survival of 60% in young patients and only 20% in the elderly CBFβ-SMMHC outcompetes CBFβ for binding to RUNX1 by direct protein-protein interaction. (wikipedia.org)
- ITC analysis of ligand binding to preQ? (rochester.edu)
- Single transcriptional and translational preQ1 riboswitches adopt similar pre-folded ensembles that follow distinct folding pathways into the same ligand-bound structure. (rochester.edu)
- Although MTX includes a lower Significantly affinity, its make use of being a ligand continues to be effective for Significantly concentrating on if a multivalent style AST-1306 strategy [28,29,is certainly applied that may offer very tight binding in comparison to a weak monovalent binding interaction. (healthandwellnesssource.org)
- 1NNP: X-ray structure of the GluR2 ligand-binding core (S1S2J) in complex with (S)-ATPA at 1.9 A resolution. (rcsb.org)
- Domain II comprises four repeats homologous to the ligand-binding portion of the LDL receptor with six conserved cysteine residues and a pentapeptide, DGSDE, which mediates ligand binding by the LDL receptor. (wikipedia.org)
- A molecule that binds to a receptor is called a ligand, and can be a protein or peptide (short protein), or another small molecule such as a neurotransmitter, hormone, pharmaceutical drug, toxin, or parts of the outside of a virus or microbe. (wikipedia.org)
- When a ligand binds to its corresponding receptor, it activates or inhibits the receptor's associated biochemical pathway. (wikipedia.org)
- They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane domain where the transmembrane domain in turn includes four transmembrane alpha helices. (wikipedia.org)
- Type 3: Kinase-linked and related receptors (see "Receptor tyrosine kinase", and "Enzyme-linked receptor") - They are composed of an extracellular domain containing the ligand binding site and an intracellular domain, often with enzymatic-function, linked by a single transmembrane alpha helix. (wikipedia.org)
- They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains the AF1(activation function 1) region. (wikipedia.org)
- Ligand binding is an equilibrium process. (wikipedia.org)
- All RTKs consists of an extracellular ligand binding region, a single transmembrane helix and a cytoplasmic region (the tyrosine kinase domain). (wikipedia.org)
- Ligand binding to the extracellular domain induces dimerization. (wikipedia.org)
- It is not involved directly in TGF-beta signal transduction but by binding to various member of the TGF-beta superfamily at the cell surface it acts as a reservoir of ligand for TGF-beta receptors. (wikipedia.org)
- UBF recruits and binds a protein complex called SL1 in humans (or TIF-IB in mouse), composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs). (wikipedia.org)
- In the presence of a TATA-less promoter, TBP binds with the help of TBP-associated factors (TAFs). (wikipedia.org)
- TAFs, however, add promoter selectivity, especially if there is no TATA box sequence for TBP to bind to. (wikipedia.org)
- To understand the molecular mechanisms for synergy between these transcription factors in the context of chromatin, we used in vivo footprinting to study the requirements for protein binding to Edelta within wild-type and mutant versions of a human TCR delta minilocus in stably transfected Jurkat cells. (duke.edu)
- 2002). An algorithm for finding protein-DNA binding sites with applications to chromatin-immunoprecipitation microarray experiments. (core.ac.uk)
- Guo C, Hu Q, Yan C, Zhang J. Multivalent binding of the ETO corepressor to E proteins facilitates dual repression controls targeting chromatin and the basal transcription machinery. (nih.gov)
- We provide several lines of evidence that TF binding at plant HOT regions is functional, in contrast to that in animals, and not merely the result of accessible chromatin. (plantcell.org)
- Distal bound regions are under purifying selection as well and are enriched for a chromatin state showing regulation by the Polycomb repressive complex. (plantcell.org)
- Transcription factors bind to DNA from the LCR to the promoter in an orderly fashion using non-DNA-binding proteins and chromatin modifiers. (wikipedia.org)
- Acetylated histones, the octomeric protein cores of nucleosomes, represent a type of epigenetic marker within chromatin. (wikipedia.org)
- This idea is supported by direct contact in vitro between activators and GTFs, including TATA box-binding protein (TBP) ( 2 ), TFIIB ( 3 ), TFIIF ( 4 ), TFIIH ( 5 ), as well as the Rpb5 subunit of RNAPII ( 6 ), and TBP-associated factors ( 7 , 8 ). (pnas.org)
- The presence of XPC-RAD23B is required for assembly of the other core NER factors and progression through the NER pathway both in vitro and in vivo. (wikipedia.org)
- Binds DNA in a sequence-specific manner. (uniprot.org)
- In molecular biology, a transcription factor (TF) (or sequence-specific DNA-binding factor) is a protein that controls the rate of transcription of genetic information from DNA to messenger RNA, by binding to a specific DNA sequence. (wikipedia.org)
- Transcription is carried out by RNA polymerase but its specificity is controlled by sequence-specific DNA binding proteins called transcription factors. (wikipedia.org)
- Pol I requires no TATA box in the promoter, instead relying on an upstream control element (UCE) located between −200 and −107, and a core element located between −45 and +20. (wikipedia.org)
- The UBF dimer contains several high-mobility-group boxes (HMG-boxes) that introduce loops into the upstream region, allowing the UCE and the core elements to come into contact. (wikipedia.org)
- It is typically 10 nucleotides long, and is present -30 to -20 nucleotides upstream from the transcription start site, in the core promoter region. (wikipedia.org)
- The upstream regulators of the core Hpo/Wts kinase cascade include the transmembrane protein Fat and several membrane-associated proteins. (wikipedia.org)
- It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC1 to deaminate the upstream cytidine. (wikipedia.org)
- A stem loop structure upstream of the terminator region pauses the RNAP, when ρ-factor reaches the RNAP, it causes RNAP to dissociate from the DNA, terminating transcription. (wikipedia.org)
- Among its related pathways are ATF-2 transcription factor network and Dual hijack model of Vif in HIV infection . (genecards.org)
- Both pathways lead to the activation of factor X (the common pathway) which combines with activated factor V in the presence of calcium and phospholipid to produce thrombin (thromboplastin activity). (wikipedia.org)
- HMGB1-LPS complex activates TLR4, and causes the binding of adapter proteins (MyD88 and others), leading to signal transduction and the activation of various signaling cascades. (wikipedia.org)
- This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. (wikipedia.org)
- Many of the mutations we identified interfered with molecular signaling or epigenetic factors. (ascopost.com)
- However, we identified preferential association of CBL exon8/9 mutations with core binding factor leukemias and 11q aberrations. (aacrjournals.org)
- Here we illustrate mechanistic advances in our understanding of familial MDS/AML syndromes caused by germline mutations of hematopoietic transcription factors. (jci.org)
- The point mutations of CBFalpha related to the aforementioned diseases were also mapped and their effect on DNA binding is discussed. (rcsb.org)
- Mutations arising within a CRE can generate expression variance by changing the way TFs bind. (wikipedia.org)
- CBF beta p22.0, CBF beta p21.5, and CBF beta p17.6 do not by themselves bind the core site. (asm.org)
- HDX-MS reveals that while the dominant structural changes occur proximal to the cofactor-binding site, rearrangements on cofactor binding are evident throughout the protein, predominantly attributable to allosteric conformational tightening, consistent with IM-MS data. (nature.com)
- Structural analysis of a class III preQ1 riboswitch reveals an aptamer distant from a ribosome-binding site regulated by fast dynamics. (rochester.edu)
- Solution structure determination of core binding factor ß(1-141) and map of CPFα binding site. (dartmouth.edu)
- The CBFα binding site on CBFß was mapped by chemical shift perturbation analysis . (dartmouth.edu)
- Using this site-specific ChIP, we were able to confirm four previously described Sp1 binding sites within egfr promoter region to be occupied by Sp1 in vivo. (biomedsearch.com)
- The consensus binding site for CBF has been identified to be a 7 bp sequence PyGPyGGTPy. (wikipedia.org)
- In bacteria The promoter is recognized by RNA polymerase and an associated sigma factor, which in turn are often brought to the promoter DNA by an activator protein's binding to its own DNA binding site nearby. (wikipedia.org)
- The core promoter marks the start site for transcription by binding RNA polymerase and other proteins necessary for copying DNA to RNA. (wikipedia.org)
- Proliferation of the cancer cell at the metastatic site Formation of a micrometastasis inside the secondary site Progressive colonization, forming a life-threatening metastasis The potential of a tumor cell to metastasize depends on its microenvironment, or "niche" interactions with local factors promoting tumor-cell growth, survival, angiogenesis, invasion and metastasis. (wikipedia.org)
- A transcription factor and its associated transcription mediator complex must be attached to a DNA binding site called a promoter region before RNAP can initiate the DNA unwinding at that position. (wikipedia.org)
- Binding of effector molecules may be affected in a similar manner if the phosphorylated residue makes part of the allosteric site. (wikipedia.org)
- Binding utilizes an enzyme-regulated 'off-on' switch in which active epithelial heparanase (HPSE) cleaves off heparan sulfate to expose a binding site in the N-terminal region of syndecan-1's core protein. (wikipedia.org)
- Functionality and conservation of the W-box element across plant species has been shown by gel shift experiments, random binding site selection, yeast one-hybrid screens and co-transfection assays performed with many different WRKY proteins. (wikipedia.org)
- ErEN cleavage site V -cccaacgggcccu-ccucccc-ucc/u alpha globin (HBA1 or HBA2, NM_000517.1) cccca-----cccu-cuuccccaa lipoxygenase (ALOX15, NM_001140) -ccca----gcccacuuuuccccaa alpha(I)-collagen (COL1A1, NM_000088) cucca---uccccu----ucuccaa tyroilsmochie hydroxylase (TH, NM_000360) ycca 1-5 cccw1-4 ucycc : consensus (Holcik and Liebhaber, 1997) There is some debate as to the number and identity of trans-acting factors recruited to the C-rich element. (wikipedia.org)
- RNA polymerase, assisted by one or more general transcription factors, then selects a transcription start site in the transcription bubble, binds to an initiating NTP and an extending NTP (or a short RNA primer and an extending NTP) complementary to the transcription start site sequence, and catalyzes bond formation to yield an initial RNA product. (wikipedia.org)
- Methylation has been shown to functionally impact Sp1 sites as well as a CREB binding site. (wikipedia.org)
- KLF15 binding site in the HSD17B5 promoter leading to the upregulation of testosterone production. (wikipedia.org)
- The LRP5 promoter has a KLF15 binding site. (wikipedia.org)
- It was specifically found that the circadian-driven transcription factor 'CLOCK' binds to the PFKFB3 promoter at a genuine 'E-box' site to increase transcription in cancer cells. (wikipedia.org)
- Its interactions with HIF-1α rely on a mobile loop region that helps to enclose the hydroxylation site and helps to stabilize binding of both iron and 2-oxyglutarate. (wikipedia.org)