Core Binding Factor beta Subunit: A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factors: Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor alpha Subunits: A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor Alpha 2 Subunit: A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.Transcription Factor AP-2: A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.Core Binding Factor Alpha 1 Subunit: A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.Smooth Muscle Myosins: Myosin type II isoforms found in smooth muscle.Transforming Growth Factor beta: A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.Technology, Radiologic: The application of scientific knowledge or technology to the field of radiology. The applications center mostly around x-ray or radioisotopes for diagnostic and therapeutic purposes but the technological applications of any radiation or radiologic procedure is within the scope of radiologic technology.Chromosomes, Human, Pair 16: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Chromosome Inversion: An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Acclimatization: Adaptation to a new environment or to a change in the old.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Sequence Analysis, DNA: A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.Chemistry, Clinical: The specialty of ANALYTIC CHEMISTRY applied to assays of physiologically important substances found in blood, urine, tissues, and other biological fluids for the purpose of aiding the physician in making a diagnosis or following therapy.High-Throughput Nucleotide Sequencing: Techniques of nucleotide sequence analysis that increase the range, complexity, sensitivity, and accuracy of results by greatly increasing the scale of operations and thus the number of nucleotides, and the number of copies of each nucleotide sequenced. The sequencing may be done by analysis of the synthesis or ligation products, hybridization to preexisting sequences, etc.Intercostal Nerves: The ventral rami of the thoracic nerves from segments T1 through T11. The intercostal nerves supply motor and sensory innervation to the thorax and abdomen. The skin and muscles supplied by a given pair are called, respectively, a dermatome and a myotome.Genomics: The systematic study of the complete DNA sequences (GENOME) of organisms.Clinical Chemistry Tests: Laboratory tests demonstrating the presence of physiologically significant substances in the blood, urine, tissue, and body fluids with application to the diagnosis or therapy of disease.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Leukemia: A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Genes, Tumor Suppressor: Genes that inhibit expression of the tumorigenic phenotype. They are normally involved in holding cellular growth in check. When tumor suppressor genes are inactivated or lost, a barrier to normal proliferation is removed and unregulated growth is possible.Cell Line, Tumor: A cell line derived from cultured tumor cells.Leukemia, Myeloid: Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.Leukemia, Lymphocytic, Chronic, B-Cell: A chronic leukemia characterized by abnormal B-lymphocytes and often generalized lymphadenopathy. In patients presenting predominately with blood and bone marrow involvement it is called chronic lymphocytic leukemia (CLL); in those predominately with enlarged lymph nodes it is called small lymphocytic lymphoma. These terms represent spectrums of the same disease.Tumor Suppressor Proteins: Proteins that are normally involved in holding cellular growth in check. Deficiencies or abnormalities in these proteins may lead to unregulated cell growth and tumor development.Trabecular Meshwork: A porelike structure surrounding the entire circumference of the anterior chamber through which aqueous humor circulates to the canal of Schlemm.Gene Expression Profiling: The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.Transcriptome: The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.False Negative Reactions: Negative test results in subjects who possess the attribute for which the test is conducted. The labeling of diseased persons as healthy when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Reproducibility of Results: The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.Proto-Oncogene Protein c-ets-2: A ubiquitously expressed ets proto-oncogene protein that may play a role in regulation of CELL PROLIFERATION and CELL DIFFERENTIATION.Proto-Oncogene Protein c-ets-1: An ets proto-oncogene expressed primarily in adult LYMPHOID TISSUE; BRAIN; and VASCULAR ENDOTHELIAL CELLS.Proto-Oncogene Proteins c-ets: A family of transcription factors that share a unique DNA-binding domain. The name derives from viral oncogene-derived protein oncogene protein v-ets of the AVIAN ERYTHROBLASTOSIS VIRUS.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Proto-Oncogenes: Normal cellular genes homologous to viral oncogenes. The products of proto-oncogenes are important regulators of biological processes and appear to be involved in the events that serve to maintain the ordered procession through the cell cycle. Proto-oncogenes have names of the form c-onc.Libraries, Digital: Libraries in which a major proportion of the resources are available in machine-readable format, rather than on paper or MICROFORM.Flight, Animal: The use of wings or wing-like appendages to remain aloft and move through the air.New HampshireLibrary Administration: Planning, organizing, staffing, direction, and control of libraries.Interlibrary LoansLibraries, MedicalLibraries: Collections of systematically acquired and organized information resources, and usually providing assistance to users. (ERIC Thesaurus, http://www.eric.ed.gov/ accessed 2/1/2008)Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Science: The study of natural phenomena by observation, measurement, and experimentation.Quality of Life: A generic concept reflecting concern with the modification and enhancement of life attributes, e.g., physical, political, moral and social environment; the overall condition of a human life.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Biology: One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.Natural Science Disciplines: The sciences dealing with processes observable in nature.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
Indispensable role of the transcription factor PEBP2/CBF in angiogenic activity of a murine endothelial cell MSS31. (1/86)Mice lacking the AML1/PEBP2alphaB/CBFa2 gene or PEBP2beta/CBFb gene exhibit a defect in definitive hematopoiesis and die in utero because of hemorrhage in the central nervous system. Hematopoiesis in the embryo is considered to be tightly associated with vascular development. Here we examined whether PEBP2/CBF plays any role in angiogenesis besides that in definitive hematopoiesis. We found that AML1/PEBP2alphaB/CBFa2, PEBP2alphaA/CBFa1, and PEBP2beta/CBFb were expressed in a murine endothelial cell line MSS31. The expression of these molecules as well as the DNA binding activity of PEBP2/CBF were augmented by angiogenic growth factors such as bFGF and VEGF. Moreover, the expression of PEBP2 alpha/CBFa protein in endothelial cells was confirmed at the site of angiogenesis in vivo. To further clarify the role of PEBP2/CBF in angiogenesis, we established permanent transfectants of PEBP2 beta-MYH11 gene, one that interacts with the runt domain of the alpha subunit and deregulates PEBP2/CBF in a dominant interfering manner. Proliferation, migration, and tube formation of the PEBP2 beta-MYH11 transfectants were significantly reduced in comparison with those activities of the mock transfectants. These results suggest that transcription factor PEBP2/CBF plays an important role in angiogenesis. (+info)
Zebrafish homolog of the leukemia gene CBFB: its expression during embryogenesis and its relationship to scl and gata-1 in hematopoiesis. (2/86)Mammalian CBFB encodes a transcription factor (CBF beta) that in combination with CBF alpha 2 binds to specific DNA sequences and regulates expression of a number of hematopoietic genes. CBFB is associated with human leukemias through a chromosome 16 inversion and is essential for definitive hematopoiesis during mouse embryo development. We have isolated a zebrafish cbfb complementary DNA (cDNA) clone from a zebrafish kidney cDNA library. This cbfb is highly homologous to human and mouse CBFB/Cbfb genes at both the DNA and protein level. In biochemical analyses, cbfbeta binds to human CBF alpha 2 and enhances its DNA binding. During zebrafish development, cbfb is expressed in the lateral plate mesoderm at tail bud stage and in the intermediate cell mass (ICM, the location of embryonic hematopoiesis) between the 21- to 26-somite stages. The cbfb is also expressed in Rohon-Beard cells, cranial nerve ganglia, hindbrain, retina, branchial arches, jaw, and fin buds. Expression of cbfb is decreased or absent in the ICM and Rohon-Beard cells in some hematopoietic mutants and is unaffected in others. We have also analyzed the expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors to cbfb in zebrafish hematopoiesis. Our results indicate that cbfb is expressed in early hematopoietic progenitors and that its expression pattern in the hematopoietic mutants is similar to that of scl. (Blood. 2000;96:4178-4184) (+info)
Core-binding factor beta (CBFbeta), but not CBFbeta-smooth muscle myosin heavy chain, rescues definitive hematopoiesis in CBFbeta-deficient embryonic stem cells. (3/86)Core-binding factor beta (CBFbeta) is the non-DNA-binding subunit of the heterodimeric CBFs. Genes encoding CBFbeta (CBFB), and one of the DNA-binding CBFalpha subunits, Runx1 (also known as CBFalpha2, AML1, and PEBP2alphaB), are required for normal hematopoiesis and are also frequent targets of chromosomal translocations in acute leukemias in humans. Homozygous disruption of either the Runx1 or Cbfb gene in mice results in embryonic lethality at midgestation due to hemorrhaging in the central nervous system, and severely impairs fetal liver hematopoiesis. Results of this study show that Cbfb-deficient mouse embryonic stem (ES) cells can differentiate into primitive erythroid colonies in vitro, but are impaired in their ability to produce definitive erythroid and myeloid colonies, mimicking the in vivo defect. Definitive hematopoiesis is restored by ectopic expression of full-length Cbfb transgenes, as well as by a transgene encoding only the heterodimerization domain of CBFbeta. In contrast, the CBFbeta-smooth muscle myosin heavy chain (SMMHC) fusion protein generated by the inv(16) associated with acute myeloid leukemias (M4Eo) cannot rescue definitive hematopoiesis by Cbfb-deficient ES cells. Sequences responsible for the inability of CBFbeta-SMMHC to rescue definitive hematopoiesis reside in the SMMHC portion of the fusion protein. Results also show that the CBFbeta-SMMHC fusion protein transdominantly inhibits definitive hematopoiesis, but not to the same extent as homozygous loss of Runx1 or Cbfb. CBFbeta-SMMHC preferentially inhibits the differentiation of myeloid lineage cells, while increasing the number of blastlike cells in culture. (+info)
Primary chromosomal rearrangements of leukemia are frequently accompanied by extensive submicroscopic deletions and may lead to altered prognosis. (4/86)BCR/ABL fluorescent in situ hybridization study of chronic myeloid leukemia (CML) and Philadelphia(+) (Ph(+)) acute lymphoid leukemia (ALL) indicated that approximately 9% of patients exhibited an atypical hybridization pattern consistent with a submicroscopic deletion of the 5' region of ABL and the 3' region of the BCR genes on the 9q(+) chromosome. The CML patients with deletions had a shorter survival time and a high relapse rate following bone marrow transplant. Since deletions are associated with both Ph(+) CML and ALL, it seemed probable that other leukemia-associated genomic rearrangements may also have submicroscopic deletions. This hypothesis was confirmed by the detection of deletions of the 3' regions of the CBFB and the MLL genes in AML M4 patients with inv(16) and in patients with ALL and AML associated with MLL gene translocations, respectively. In contrast, analysis of the AML M3 group of patients and AML M2 showed that similar large deletions were not frequently associated with the t(15;17) or t(8;21) translocations. Analysis of sequence data from each of the breakpoint regions suggested that large submicroscopic deletions occur in regions with a high overall density of Alu sequence repeats. These findings are the first to show that the process of deletion formation is not disease specific in leukemia and also implicate that the presence of repetitive DNA in the vicinity of breakpoint regions may facilitate the generation of submicroscopic deletions. Such deletions could lead to the loss of one or more genes, and the associated haploinsufficiency may result in the observed differences in clinical behavior. (Blood. 2001;97:3581-3588) (+info)
Cloning and expression pattern of a novel PEBP2 beta-binding protein (charged amino acid rich leucine zipper-1[Crl-1]) in the mouse. (5/86)PEBP2 beta/Cbf beta is the beta subunit of PEBP2/Cbf, which has been demonstrated to have important biological activities in hematopoiesis and osteogenesis. However, PEBP2 beta is ubiquitously expressed, suggesting that PEBP2 has other additionally important physiological activities. In an effort to elucidate other possible functions for PEBP2, we have isolated a novel gene that encodes a PEBP2 beta-interacting protein from a mouse cDNA library. We have called this gene Crl-1 for charged amino acid rich leucine zipper-1 (Crl-1) because it is rich in charged amino acids and contains a putative leucine zipper region. Expression studies in a 17.5 days post-coitum mouse embryo demonstrated Crl-1 expression mainly in the olfactory bulb and cerebral cortex. Post-natally, Crl-1 expression was additionally observed in the cerebellar cortex with strong expression in the hippocampus. These findings show that this novel PEBP2 beta-interacting protein is expressed mainly in subsets of neuronal cells, suggesting that Crl-1 plays some role in the developing mouse brain. (+info)
Role of Cbfb in hematopoiesis and perturbations resulting from expression of the leukemogenic fusion gene Cbfb-MYH11. (6/86)Core-binding factor beta (CBFbeta) and CBFalpha2 form a heterodimeric transcription factor that plays an important role in hematopoiesis. The genes encoding either CBFbeta or CBFalpha2 are involved in chromosomal rearrangements in more than 30% of cases of acute myeloid leukemia (AML), suggesting that CBFbeta and CBFalpha2 play important roles in leukemogenesis. Inv(16)(p13;q22) is found in almost all cases of AML M4Eo and results in the fusion of CBFB with MYH11, the gene encoding smooth muscle myosin heavy chain. Mouse embryos heterozygous for a Cbfb-MYH11 knock-in gene lack definitive hematopoiesis, a phenotype shared by Cbfb(-/-) embryos. In this study we generated a Cbfb-GFP knock-in mouse model to characterize the normal expression pattern of Cbfbeta in hematopoietic cells. In midgestation embryos, Cbfbeta was expressed in populations enriched for hematopoietic stem cells and progenitors. This population of stem cells and progenitors was not present in mouse embryos heterozygous for the Cbfb-MYH11 knock-in gene. Together, these data suggest that Cbfb-MYH11 blocks embryonic hematopoiesis at the stem-progenitor cell level and that Cbfb is essential for the generation of hematopoietic stem and progenitor cells. In adult mice, Cbfbeta was expressed in stem and progenitor cells, as well as mature myeloid and lymphoid cells. Although it was expressed in erythroid progenitors, Cbfbeta was not expressed during the terminal stages of erythropoiesis. Our data indicate that Cbfb is required for myeloid and lymphoid differentiation; but does not play a critical role in erythroid differentiation. (+info)
Distinct roles for c-Myb and core binding factor/polyoma enhancer-binding protein 2 in the assembly and function of a multiprotein complex on the TCR delta enhancer in vivo. (7/86)Enhancers and promoters within TCR loci functionally collaborate to modify chromatin structure and to confer accessibility to the transcription and V(D)J recombination machineries during T cell development in the thymus. Two enhancers at the TCRalphadelta locus, the TCR alpha enhancer and the TCR delta enhancer (Edelta), are responsible for orchestrating the distinct developmental programs for V(D)J recombination and transcription of the TCR alpha and delta genes, respectively. Edelta function depends critically on transcription factors core binding factor (CBF)/polyoma enhancer-binding protein 2 (PEBP2) and c-Myb as measured by transcriptional activation of transiently transfected substrates in Jurkat cells, and by activation of V(D)J recombination within chromatin-integrated substrates in transgenic mice. To understand the molecular mechanisms for synergy between these transcription factors in the context of chromatin, we used in vivo footprinting to study the requirements for protein binding to Edelta within wild-type and mutant versions of a human TCR delta minilocus in stably transfected Jurkat cells. Our data indicate that CBF/PEBP2 plays primarily a structural role as it induces a conformational change in the enhanceosome that is associated with augmented binding of c-Myb. In contrast, c-Myb has no apparent affect on CBF/PEBP2 binding, but is critical for transcriptional activation. Thus, our data reveal distinct functions for c-Myb and CBF/PEBP2 in the assembly and function of an Edelta enhanceosome in the context of chromatin in vivo. (+info)
Core binding factor genes and human leukemia. (8/86)BACKGROUND: The core binding factor (CBF) transcription complex, consisting of the interacting proteins RUNX1 and CBFb, is essential for normal hematopoiesis. Recent studies have shown that mutations and gene rearrangements involving this complex are frequently implicated in leukemogenesis. Understanding the molecular events leading to the disruption of CBF has provided important insights into our understanding of the normal regulatory pathways that control hematopoiesis and has begun to reveal how alterations in these pathways induce leukemia. INFORMATION SOURCES: Both authors are involved in the identification and characterization of chromosomal abnormalities associated with hematologic malignancy. This has led to contributions to multicenter clinical and laboratory investigations as well as publications in peer-reviewed journals. All of the references cited in this review are published in journals covered by Medline. State of the Art. The core binding factor (CBF) is a heterodimeric transcription factor composed of the RUNX1 and CBFb subunits. RUNX1 is the DNA binding element of the complex and its affinity is greatly increased in the presence of CBFb. Knock-out studies in mice have demonstrated that both RUNX1 and CBFb are necessary for definitive hematopoiesis. Furthermore, reciprocal chromosomal translocations involving both partners have been directly implicated in leukemogenesis. Evidence is now emerging that at least some of the resulting fusion proteins, namely ETV6-RUNX1, RUNX1-MTG8 and CBFb-MYH11 dominantly inhibit the function of native CBF by recruiting transcriptional co-repressor complexes. However, knock-in studies have shown that whilst expression of these fusion genes may disrupt normal hematopoiesis, this, by itself, is not sufficient for the subsequent development of leukemia. Mutations of RUNX1 have been identified in familial platelet disorder (FDP), in which there is a congenital predisposition to the development of AML and heterozygous point mutations have been identified in the RUNX1 gene in some leukemias. Moreover, a small number of cases have been reported in which amplification of RUNX1 has been detected in childhood ALL suggesting mechanisms other than loss of function, such as gene dosage may also play a role. CONCLUSIONS: Understanding the role CBF plays in normal hematopoiesis and hematologic malignancies has provided critical reagents for the accurate identification of the broad group of leukemias harboring alterations of CBF. The application of these molecular approaches has already shown an impact on the clinical management of these patients and as more information becomes available, the ability to tailor therapy to improve each patient's chance of a cure becomes feasible. (+info)
Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by the alpha subunit as the ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family ...
... core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5'-pygpyggt-3', of a number of enhancers and ... The protein is a heterodimer of alpha- and beta-subunits. The alpha-subunit binds DNA as a monomer, and appears to have a role ... In addition to the highly conserved Runt domain, the AML-1 gene product carries a putative ATP-binding site (GRSGRGKS), and has ... highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit ...
This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the ... 1997). "Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1". ... "Entrez Gene: GABPB2 GA binding protein transcription factor, beta subunit 2". Sawada J, Goto M, Watanabe H, et al. (1995). " ... GA-binding protein subunit beta-1 is a protein that in humans is encoded by the GABPB1 gene. ...
A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a ... Core binding factor RUNX1 RUNX2 GRCh38: Ensembl release 89: ENSG00000020633 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... Jin YH, Jeon EJ, Li QL, Lee YH, Choi JK, Kim WJ, Lee KY, Bae SC (Jul 2004). "Transforming growth factor-beta stimulates p300- ... Runt-related transcription factor 3 is a protein that in humans is encoded by the RUNX3 gene. This gene encodes a member of the ...
The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) subunits) binds a sigma ... Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... Different sigma factors are utilized under different environmental conditions. These specialized sigma factors bind the ... Sigma factors in E. coli: σ70(RpoD) - σA - the "housekeeping" sigma factor or also called as primary sigma factor, transcribes ...
These core binding factors, or Nuclear Factors (NF-Y), are composed of three subunits - NF-YA, NF-YB, and NF-YC. Whereas in ... This is composed of three alpha-helices separated by two beta strand-loop domains. Similar to NF-YA, NF-YB has been shown to ... It is essential to the transcription that these core binding factors (also referred to as nuclear factor Y or NF-Y) are able to ... the core binding factor (CBF)-DNA complex retains a high degree of conservation within the CCAAT binding motif, as well as the ...
This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that ... pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding ... TFIIA-alpha and beta-like factor is a protein that in humans is encoded by the GTF2A1L gene. The assembly and stability of the ... 2001). "TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general ...
The beta subunits are responsible for binding the RNAP to the DNA, preventing premature dissociation. In Escherichia coli, the ... As noted above in RNA Polymerase and Sigma Factors, the beta clamp proteins within the RNAP complex have a higher initial ... Eubacterial RNAP holoenzymes consist of a core with four major subunits α2 ββ'. In cyanobacteria, β' is formed from two smaller ... In E. coli, a repressor binds the DNA operon and dislodges RNAP due to the loosely bound beta clamp, whereas in Synechocystis, ...
... binds to the beta-propeller of the DDB1 adaptor protein which interacts with numerous DDB1-CUL4-Associated Factors (DCAFs ... CRL4Cdt2 also degrades PCNA-bound PR-Set7/SET8, which is a histone 4 methyltransferase, and the p12 subunit of DNA polymerase δ ... RBX1 is a core component of Cullin-RING ubiquitin ligase (CRL) complexes and functions to recruit E2 ubiquitin conjugating ... Recent reports show that IMiDs bind to CRL4CRBN and promote the degradation of IKZN1 and IKZN3 transcription factors, which are ...
Transcription factor II B
The first transcription factor to bind the DNA is TFIID, which binds via the TBP subunit to the TATA box. TFIIB then binds to ... TFIIB-related factor) which also contains a conserved zinc ribbon and C terminal core. However, the structure diverges in the ... It forms a beta sheet and an ordered loop that helps with the stability of the structure when transcription is initiated. The ... The binding of TBP to DNA forms a 90° kink in the DNA and allows the TFIIB to clamp the TBP tightly to the DNA. The binding of ...
... chimeric transcript that encodes a protein consisting of the first 165 residues from the N-terminus of core-binding factor beta ... Myosin-11 is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light ... 1998). "The chimeric protein, PEBP2 beta/CBF beta-SMMHC, disorganizes cytoplasmic stress fibers and inhibits transcriptional ... chain subunits. It is a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ...
This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that ... Chen Y, Le Cahérec F, Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... Knight BC, High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit beta is a protein that in humans is encoded by the SEC61B gene. The Sec61 complex is ...
... binds to the beta-propeller of the DDB1 adaptor protein which interacts with numerous DDB1-CUL4-Associated Factors (DCAFs ... The smaller subunit of this Damaged DNA Binding protein complex is known as DDB2 and is able to directly bind DNA lesions ... RBX1 is a core component of Cullin-RING ubiquitin ligase (CRL) complexes and functions to recruit E2 ubiquitin conjugating ... Recent reports show that IMiDs bind to CRL4CRBN and promote the degradation of IKZN1 and IKZN3 transcription factors, which are ...
Sec61 alpha 1
This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. GRCh38: ... Chen Y; Le Cahérec F; Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... Knight BC; High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ...
NusG binds to termination factor Rho and to RNAP. It stimulates the rate of transcription elongation and is required for the ... antitermination change highly conserved amino acids located in a cysteine-rich amino-proximal domain of the RNAP beta' subunit ... The assembly of NusB, S10, and NusG onto the core complex involves nt 2 to 7 of lambda BOXA (CGCUCUUACACA), as well as the ... An additional factor or factors that can be supplied by a cellular extract are required, but their identities are unknown. ...
Ruediger R, Fields K, Walter G (Jan 1999). "Binding specificity of protein phosphatase 2A core enzyme for regulatory B subunits ... "Physical and functional interactions between type I transforming growth factor beta receptors and Balpha, a WD-40 repeat ... It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, ... "Physical and functional interactions between type I transforming growth factor beta receptors and Balpha, a WD-40 repeat ...
The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are ... composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase ... It also binds closely to the E3 ubiquitin ligase MDM2, which is a regulator of the degradation of p53 and retinoblastoma ... Accordingly, gene expression by degradation of transcription factors, such as p53, c-Jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, ...
... a subunit of the Mediator complex, which is required for the activity of the enhancer-binding protein Sp1. Elongin A, a subunit ... 2011). "Human mediator subunit MED26 functions as a docking site for transcription elongation factors". Cell. 146 (1): 92-104. ... The hydrophobic core residues of helices 2, 3, and 4 are well conserved among TFIIS domains, although helix 1 is less conserved ... Mo X, Kowenz-Leutz E, Xu H, Leutz A (2004). "Ras induces mediator complex exchange on C/EBP beta". Mol. Cell. 13 (2): 241-50. ...
Mechanistic target of rapamycin
... signaling events downstream of mTOR cooperate to mediate the effects of amino acids and insulin on initiation factor 4E-binding ... Kougias DG, Nolan SO, Koss WA, Kim T, Hankosky ER, Gulley JM, Juraska JM (April 2016). "Beta-hydroxy-beta-methylbutyrate ... As a core component of mTORC2, mTOR also functions as a tyrosine protein kinase that promotes the activation of insulin ... MTOR is the catalytic subunit of two structurally distinct complexes: mTORC1 and mTORC2. Both complexes localize to different ...
It belongs to the thioredoxin superfamily fold which is defined by a beta-sheet core surrounded by alpha-helices. The active ... This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite ... Li S, Rosen BP, Borges-Walmsley MI, Walmsley AR (July 2002). "Evidence for cooperativity between the four binding sites of ... The arsC family also comprises the Spx proteins which are Gram-positive bacterial transcription factors that regulate the ...
Eukaryotic ribosome (80S)
... and several eukaryotic ribosomal proteins jointly to form inter-protein beta-sheets. The ribosomal RNA core is represented as a ... which was co-crystallized with the eukaryotic 60S subunit and binds in the ribosomal E-site. The structural characterization of ... and RPL40 would be positioned in the decoding site and near the translation factor binding site, respectively. These positions ... Eukaryotic ribosomes have two unequal subunits, designated small subunit (40S) and large subunit (60S) according to their ...
... subunit, and 1 ω subunit. In bacteria, there is one general RNA transcription factor: sigma. RNA polymerase core enzyme binds ... which inhibits Bacterial transcription of DNA into mRNA by inhibiting DNA-dependent RNA polymerase by binding its beta-subunit ... RNA polymerase is called a holoenzyme when sigma subunit is attached to the core enzyme which is consist of 2 α subunits, 1 β ... In bacteria, RNA polymerase holoenzyme consists of five subunits: 2 α subunits, 1 β subunit, 1 β' ...
The σ-factor eventually dissociates from the core enzyme, and elongation proceeds. Promoters can differ in "strength"; that is ... The RNA polymerase transcribes the DNA (the beta subunit initiates the synthesis), but produces about 10 abortive (short, non- ... rho factor) which is a protein to stop RNA synthesis at specific sites. This protein binds at a rho utilisation site on the ... Rho-dependent termination uses a termination factor called ρ factor( ...
In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... RNA polymerase holoenzymes containing other sigma factors recognize different core promoter sequences. <-- upstream downstream ... DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. doi:10.1126/science.8248780. PMID 8248780 ... Examples include: Asthma Beta thalassemia Rubinstein-Taybi syndrome Some promoters are called constitutive as they are active ...
Tbf5 protein domain
... general transcription factor 2H subunit 5) is also known as the TTD group A (TTDA) subunit (and as Tfb5). The TTDA subunit is ... and part of a six-subunit complex of Rad3, Tfb1, Tfb2, Tfb4, Tfb5, and Ssl1 (referred to as core) In humans, the function of ... TTDA is present both bound to TFIIH, and as a free fraction that shuffles between the cytoplasm and nucleus; induction of NER- ... These proteins have a structural motif consisting of a 2-layer sandwich structure with an alpha/beta plait topology. ...
The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are ... It also have subunits that can bind with nucleotides (e.g., ATPs) in order to facilitate the association between 19S and 20S ... composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase ... Accordingly, gene expression by degradation of transcription factors, such as p53, c-Jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, ...
Complete information for CBFB gene (Protein Coding), Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two ...
Im thinking you could do an EMSA - then add a second antibody against your POI and probe for it separately. add controls - one band means your POI is part of the complex, two means its ...
Complex karyotype, older age, and reduced first-line dose intensity determine poor survival in core binding factor acute...
Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. Few prognostic markers have been identified. In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. Overall, 10-year overall survival (OS), disease-free survival (DFS), and event-free survival were 63.9%, 54.8%, and 49.9%, respectively; patients with the t(8;21) and inv(16) chromosomal rearrangements exhibited significant differences at diagnosis. Despite similarly high complete remission (CR) rate, patients with inv(16) experienced superior DFS and a high chance of achieving a second CR, often leading to prolonged OS also after relapse. We found that a complex karyotype (ie, ≥4 cytogenetic anomalies) affected survival; the KIT D816 mutation predicted worse prognosis only in patients with the t(8;21) rearrangement, whereas FLT3 mutations had no ...
The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. Runx proteins are encoded by three genes: Runx1, Runx2 , and ...
Among patients with good prognosis core binding factor AML, there is an overall survival rate of only 44%. To understand the genetic factors contributing to poor outcomes within this subgroup, Dr Chew is analysing bone marrow samples collected from 18 patients before and during treatment.. According to Dr Chew, multiple genetic abnormalities acquired during therapy are probably responsible for good prognosis core binding AML developing resistance to chemotherapy.. "To help us predict who will respond poorly to therapy, were identifying the genetic mutations occurring in patients who relapse," he said. "This information will allow us to tailor patient treatment accordingly. Currently a stem cell transplant is considered the definitive treatment and our findings will help clinicians decide if their patients AML will develop resistance and if a stem cell transplant is recommended.". Dr Chew is testing the usefulness of the genetic variations he identifies through an international ...
Dasatinib in Combination With Chemotherapy for Relapsed or Refractory Core Binding Factor Acute Myeloid Leukemia: A Phase I...
This study is examining the appropriate dose and side effects of dasatinib, when it is given with the standard of care chemotherapy for children and adolescents
1P7K: Structure of an anti-DNA fab complexed with a non-DNA ligand provides insights into cross-reactivity and molecular mimicry.
Core-binding factor, runt domain, alpha subunit 3 | definition of core-binding factor, runt domain, alpha subunit 3 by Medical...
Looking for online definition of core-binding factor, runt domain, alpha subunit 3 in the Medical Dictionary? core-binding factor, runt domain, alpha subunit 3 explanation free. What is core-binding factor, runt domain, alpha subunit 3? Meaning of core-binding factor, runt domain, alpha subunit 3 medical term. What does core-binding factor, runt domain, alpha subunit 3 mean?
core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
Core binding factor (CBF) is a heterodimeric transcription factor that is essential for a number of developmental process including hemotopoiesis and bone development. CBFs contain a DNA-binding CBFα. subunit and a non-DNA binding CBFß. subunit ...
Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
Disease, Muscle, Muscle Cells, Smooth Muscle, Smooth Muscle Cells, Cells, Heme, Hydrogen, Alkaline Phosphatase, Kidney, Kidney Disease, Osteocalcin, Upregulation, Vascular Calcification, Calcium, Heme Oxygenase, Vascular Smooth Muscle, Atherosclerosis, Bone, Core Binding Factor
PHF2라는 단백질이 뼈를 만드는 세포(조골세포)를 활성화시킨다는 사실을 처음으로 규명했다. 조골세포는 Runx2라는 단백질에 의해 분화가 조절된다. 반면, SUV39HI1라는 효소는 Runx2에 메틸기(CH3)를 붙임으로써 Runx2가 기능을 하지 못하게 하는 장식으로 분화를 방해한다. 성장이 끝난 성인들이 더 이상 키가 크지 않는 것도 SUV39HI1 효소 때문이다. 이에 착안해 Runx2에 붙어 있는 메틸기를 제거하는 방안을 연구한 결과, PHF2 단백질이 조골세포 분화를 유도함으로써, 소아의 뼈 발달 과정이나 골절 후 뼈가 새로 형성되는 과정에 작용한다는 것을 증명했다.. PHF2 단백질은 Runx2에 붙어 있는 메틸기를 제거했으며, 이후 본연의 기능을 회복한 Runx2는 조골세포의 분화를 촉진하여 다시 뼈를 만들기 시작했다. 실제 유전자 조작으로 PHF2 단백질이 과발현된 쥐를 만들어 ...
Decreased Llgl1 expression has been previously shown to be associated with increased metastatic potential, malignant phenotype, or inferior survival in a variety of solid tumors (Ohali et al., 2004; Schimanski et al., 2005; Kuphal et al., 2006). Given that Llgl1 restrains self-renewal in HSCs and that leukemia is characterized by aberrant activation of self-renewal, we assessed for a role in human AML. First, we asked whether the gene expression signature revealed by genetic inactivation of Llgl1 (Llgl1−/− signature) in HSCs was associated with any genetic subtypes of primary AML. Indeed, clustering based on the Llgl1−/− signature (Fig. 4 B) grouped AML cases into subgroups significantly associated with cytogenetic abnormalities (Fig. 4, B and C). Especially core-binding factor leukemias (t(8;21) and inv(16)) or t(15;17) clustered into specific subgroups. Moreover, the Llgl1−/− signature was most strongly correlated with group 7 based on hierarchical clustering. This group contained ...
Modulation of Runx proteins by steroid hormone receptors :: University of Southern California Dissertations and Theses
Estrogen receptor α (ER α) and androgen receptor (AR) are master transcription factors in the breast and prostate, respectively. They are commonly known in development of sexual characteristics. However, both ERα and AR have been known to be involved in breast cancer (BCa) and prostate cancer (PCa) progression, respectively. The Runx family of transcription factors plays a role in hematopoiesis (Runx1), skeletogenesis (Runx2) and neurogenesis (Runx3). In addition, Runx proteins inhibit cell cycle progression, and have been assigned tumor suppressor roles in various contexts. Because both BCa and PCa cells metastasize to bone at high frequency, investigators have interrogated the possibility that they share characteristics with osteoblasts. Indeed, BCa and PCa cells were found to have "osteomimetic" properties, including expression of Runx2 and Runx2-target genes otherwise expressed by osteoblasts. Provoked by the reported physical interaction between AR and Runx2, we initiated a study to test ...
Cells, Lead, Acute Myeloid Leukemia, Bone, Bone Marrow, Core-binding Factor, Cytogenetic, Cytogenetic Abnormalities, Disease, Leukemia, Marrow, Myeloid Leukemia, Patient, Patients, Protein Array, Proteins, Regression, Regression Analysis, Ability, Algorithm
Fluoride Action Network | Expression of core-binding factor a1 and osteocalcin in fluoride-treated fibroblasts and...
To study the effects and importance of fluoride on FBs in the development of extraperiosteal calcification and the ossification of skeletal fluorosis, the presence of the osteogenic phenotype, which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in osteoblasts (OBs) exposed to fluoride was determined. Fibroblasts and osteoblasts were exposed to different concentrations of fluoride (0, 0.0001, 0.001, 0.1, 1.0, 10.0 and 20.0mg/L F-). By using RT-PCR and ELISA, the mRNA levels of Cbfa1 and OCN were measured at 48h, and the protein levels of Cbfa1 and OCN were measured at 2, 4, 24, 48 and 72h. The data demonstrated the following: (1) The Cbfa1 protein level in fluoride-treated fibroblasts clearly increased at 48h in the groups treated with 0.0001, 0.001, 0.1, 1.0 and 20.0mg/L F-. The Cbfa1 protein level of the group treated with 10mg/L F- at 72h was higher than that of the control group. The level of Cbfa1 mRNA in the fibroblasts ...
Truncated forms of RUNX3 unlike full length protein alter cell proliferation in a TGF-β context dependent manner
The Runt related transcription factors (RUNX) are recognized as key players in suppressing or promoting tumor growth. RUNX3, a member of this family, is known as a tumor suppressor in many types of cancers, although such a paradigm was challenged by some researchers. The TGF-β pathway governs major upstream signals to activate RUNX3. RUNX3 protein consists of several regions and domains. The Runt domain is a conserved DNA binding domain and is considered as the main part of RUNX proteins since. Herein, we compared the effects of Runt domains and full-Runx3 in cell viability by designing two constructs of Runx3, including N-terminal region and Runt domain. We investigated the effect of full-Runx3, N-t, and RD on growth inhibition in AGS, MCF-7, A549, and HEK293 cell lines which are different in TGF-β sensitivity, in the absence and presence of TGF-β. The full length RUNX3 did not notably inhibit growth of these cell lines while, the N-t and RD truncates showed different trends in these cell lines.
Coupling between the 4f core binding energy and the 5f valence band occupation of elemental Pu and Pu-based compounds
A series of studies of f-electron systems based on density functional theory methods have been performed. The focus of the studies has been on magnetic and structural properties, as well as investigating ways to handle strong electron correlation in these systems.. A version of the self-interaction correction (SIC) method has been developed for a full-potential linear muffin-tin orbital method. The method is demonstrated to have the strong capabilities of previous SIC implementations, to study energetics and phase stabilities of d- and f-electron systems with localisation-delocalisation transitions, but with no geometrical constraints from the underlying band structure method. The method is applied to the high-TC superconductor CeOFeAs, in which the f-shell of the Ce atoms is argued to undergo a Mott transition to a delocalised state under pressure.. The non-collinear magnetic structures of two rare earth compounds, TbNi5 and CeRhIn5 have been studied, and in both cases the complex magnetic ...
The novel FMS-like tyrosine kinase 3 (FLT3)-N676K point mutation within the FLT3 kinase domain-1 was recently identified in 6 % of de novo acute myeloid leukemia (AML) patients with inv(16). Because FLT3-N676K was encountered almost exclusively in inv(16) AML, we investigated the transforming potential of FLT3-N676K, the cooperation between FLT3-N676K and core binding factor ß-smooth muscle myosin heavy chain (CBFß-SMMHC) (encoded by the inv(16) chimeric gene CBFB-MYH11) in inducing acute leukemia, and tested the sensitivity of FLT3-N676K-positive leukemic cells to FLT3 inhibitors. Retroviral expression of FLT3-N676K in myeloid 32D cells induced AML in syngeneic C3H/HeJ mice (n = 11/13, median latency 58 days), with a transforming activity similar to FLT3-internal tandem duplication (ITD) (n = 8/8), FLT3-TKD D835Y (n = 8/9), and FLT3-ITD-N676K (n = 9/9) mutations. Three out of 14 (21.4 %) C57BL/6J mice transplanted with FLT3-N676K-transduced primary hematopoietic progenitor cells developed ...
Interactions among the transcription factors Runx1, RORgammat and Foxp3 regulate the differentiation of interleukin 17...
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Reactome | RUNX1:CBFB:SIN3A,(SIN3B):PRMT6:HDAC1:MIR27A gene:H3K4me2,H3R2me2a-Nucleosome [nucleoplasm]
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
The pathogenesis of PDAC involves genetic alterations, such as K-ras protooncogene mutations, mutations of the p53, p16, and Smad4 tumour suppressor genes, and other less common mutations.2 In addition, there are numerous epigenetic alterations, including altered expression of several growth factors and their receptors.3 For example, PDACs overexpress all TGFβ isoforms and their receptors, and overexpression of these ligands and receptors is often associated with shortened postoperative survival of patients with pancreatic cancer.14,15. The TGFβ pathway is carefully regulated, with Smad proteins as the key component in the signal transduction pathway. In addition, other regulators, such as transcription factors, which facilitate Smad binding to target promoters, may provide routes for feedback and crosstalk.16 For example, members of the CBFA (core binding factor A) family of transcription factors act both as targets and partners of activated Smads. This family, also termed the "Runx family", ...
CATAGAGCCA GCGGGCGCGG GCGGGACGGG CGCCCCGCGG CCGGACCCAG CCAGGGCACC ACGCTGCCCG GCCCTGCGCC GCCAGGCACT TCTTTCCGGG ^1 ^11 ^21 ^31 ^41 ^51 ^61 ^71 ^81 ^91 GCTCCTAGGG ACGCCAGAAG GAAGTCAACC TCTGCTGCTT CTCCTTGGCC TGCGTTGGAC CTTCCTTTTT TTGTTGTTTT TTTTTGTTTT TCCCCTTTCT ^101 ^111 ^121 ^131 ^141 ^151 ^161 ^171 ^181 ^191 TCCTTTTGAA TTAACTGGCT TCTTGGCTGG ATGTTTTCAA CTTCTTTCCT GGCTGCGAAC TTTTCCCCAA TTGTTTTCCT TTTACAACAG GGGGAGAAAG ^201 ^211 ^221 ^231 ^241 ^251 ^261 ^271 ^281 ^291 TGCTCTGTGG TCCGAGGCGA GCCGTGAAGT TGCGTGTGCG TGGCAGTGTG CGTGGCAGGA TGTGCGTGCG TGTGTAACCC GAGCCGCCCG ATCTGTTTCG ^301 ^311 ^321 ^331 ^341 ^351 ^361 ^371 ^381 ^391 ATCTGCGCCG CGGAGCCCTC CCTCAAGGCC CGCTCCACCT GCTGCGGTTA CGCGGCGCTC GTGGGTGTTC GTGCCTCGGA GCAGCTAACC GGCGGGTGCT ^401 ^411 ^421 ^431 ^441 ^451 ^461 ^471 ^481 ^491 GGGCGACGGT GGAGGAGTAT CGTCTCGCTG CTGCCCGAGT CAGGGCTGAG TCACCCAGCT GATGTAGACA GTGGCTGCCT TCCGAAGAGT GCGTGTTTGC ^501 ^511 ^521 ^531 ^541 ^551 ^561 ^571 ^581 ^591 ATGTGTGTGA CTCTGCGGCT GCTCAACTCC CAACAAACCA GAGGACCAGC ...
Runx1 mediates the development of the granular convoluted tubules in the submandibular glands "The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription factor (RUNX) proteins (RUNX1, 2 and 3). This result clearly demonstrates that the Runx /Cbfb signaling pathway is indispensable in the development of the GCTs. However, it is not clear which of the RUNX proteins plays useful role in the development of the GCTs by activating the Runx /Cbfb signaling pathway. Past studies have revealed that the Runx /Cbfb signaling pathway plays important roles in various aspects of development and homeostatic events. Moreover, the Runx genes have different temporospatial ...
CBFB core-binding factor subunit beta [Homo sapiens (human)] - Gene - NCBI
SL3-3 enhancer factor 1 subunit beta. SL3/AKV core-binding factor beta subunit. core-binding factor beta subunit. polyomavirus ... core-binding factor subunit beta. Names. CBF-beta. PEA2-beta. PEBP2-beta. SL3-3 enhancer factor 1 beta subunit. ... CBFB core-binding factor subunit beta [Homo sapiens] CBFB core-binding factor subunit beta [Homo sapiens]. Gene ID:865 ... core-binding factor subunit betaprovided by HGNC. Primary source. HGNC:HGNC:1539 See related. Ensembl:ENSG00000067955 MIM: ...https://www.ncbi.nlm.nih.gov/gene/865
Core-binding factor subunit beta
... is not required for non-primate lentiviral Vif-mediated APOBEC3 degradation.. ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the ... The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the ... produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion ...https://pharos.nih.gov/idg/targets/Q13951
Core-binding factor beta subunit (CBFB) (CBFbeta) - Molecular Target Profile - BioCentury - BCIQ
Comprehensive view of all products targeting Core-binding factor beta subunit (CBFB) (CBFbeta). Includes lead product status, ... Core-binding factor b-subunit (CBFB; CBFb); runt-related transcription factor 1 (RUNX1) In vitro and mouse studies suggest ... Therapeutics: Core-binding factor β subunit (CBFB; CBF-β); myosin heavy chain 11 smooth muscle (MYH11; SMMHC); runt-related ... core-binding factor b-subunit (CBFB; CBFb) In vitro and mouse studies suggest the small molecule kartogenin could help treat ...https://bciq.biocentury.com/targets/core-binding_factor_beta_subunit
CBFB Gene - GeneCards | PEBB Protein | PEBB Antibody
Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - ... core binding factor to the enhancer of T cell receptor genes,beta subunit,altered in acute myeloid leukemia (AML-M4Eo) with ... CBFB (Core-Binding Factor Beta Subunit) is a Protein Coding gene. Diseases associated with CBFB include Acute Myeloid Leukemia ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=CBFB
Anti-nuclear transcription factor Y subunit beta Antibody Products | Biocompare.com
Compare Anti-nuclear transcription factor Y subunit beta Antibody Products from leading suppliers on Biocompare. View ... anti-Core-Binding Factor, beta Subunit (CBFB) (Internal Region) antibody *. Applications: WB, ELISA ... Your search returned 139 nuclear transcription factor Y subunit beta Antibodies across 25 suppliers. ... Anti-nuclear transcription factor Y subunit beta Antibody Products. Anti-nuclear transcription factor Y subunit beta Antibody ...https://www.biocompare.com/pfu/110447/soids/2267297/Antibodies/nuclear_transcription_factor_Y_subunit_beta
RCSB PDB - 1IO4: CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN-CBFBETA CORE DOMAIN HETERODIMER AND C/EBPBETA BZIP...
CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN-CBFBETA CORE DOMAIN HETERODIMER AND C/EBPBETA BZIP HOMODIMER BOUND TO A ... CORE-BINDING FACTOR, BETA SUBUNIT. D. 141. Mus musculus. Mutation(s): 0 Gene Names: Cbfb (Pebp2b, Pebpb2). ... The core binding factor (CBF) heterodimeric transcription factors comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta ... The core binding factor (CBF) heterodimeric transcription factors comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta ...https://www.rcsb.org/structure/1IO4
Sequence Similarity - 3WTU: Crystal structure of the complex comprised of ETS1 (V170A), RUNX1, CBFBETA, and the...
Core-binding factor subunit beta protein, length: 142 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ID ... Runt-related transcription factor 1 protein, length: 204 (BLAST) Sequence Similarity Cutoff. Rank. Chains in Cluster. Cluster ...https://www.rcsb.org/pdb/explore/sequenceCluster.do?structureId=3WTU
ENSDARG00000040917 - Zebrafish Mutation Project - Wellcome Sanger Institute
core-binding factor, beta subunit [Source:HGNC Symbol;Acc:1539]. Mouse Orthologue:. Cbfb. Mouse Description:. core binding ... core-binding factor subunit beta [Source:RefSeq peptide;Acc:NP_954679]. Human Orthologue:. CBFB. Human Description:. ...http://www.sanger.ac.uk/sanger/Zebrafish_Zmpgene/ENSDARG00000040917
Regulation of Lipid Metabolism by PPARalpha | www.antikoerper-online.de
CBFB - Core-Binding Factor, beta Subunit: CBFB Antikörper CBFB Proteine CREBBP - CBP: CREBBP Antikörper CREBBP ELISA Kits ... NFYB (Nuclear Transcription Factor Y, beta): NFYB Antikörper NFYB Proteine NFYC - Nuclear Transcription Factor Y, gamma: NFYC ... SP1 (Sp1 Transcription Factor): SP1 Antikörper SP1 ELISA Kits SP1 Proteine SREBF1 (Sterol Regulatory Element Binding ... MED12 (Mediator Complex Subunit 12): MED12 Antikörper MED12 ELISA Kits MED13 (Mediator Complex Subunit 13): MED13 Antikörper ...https://www.antikoerper-online.de/regulation-of-lipid-metabolism-by-pparalpha-pathway-66/
CBFB - Wikipedia
Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by the alpha subunit as the ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family ...https://en.wikipedia.org/wiki/CBFB
Recombinant Human CBFB 293 Cell Lysate CBFB-7815HCL - Creative BioMart
CBFB; core-binding factor, beta subunit; core-binding factor subunit beta; PEBP2B; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 ... enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; polyomavirus ... Antigen standard for core-binding factor, beta subunit (CBFB), transcript variant 2 is a lysate prepared from HEK293T cells ... enhancer-binding protein 2 beta subunit; polyomavirus enhancer binding protein 2, beta subunit;. ...https://www.creativebiomart.net/description_409466_318.htm
core-binding factor, beta subunit. External References: Wikipedia GeneCards HUGO COSMIC Google Scholar NCBI Description of CBFB ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the ... The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the ... produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion ...http://tumorportal.org/view?geneSymbol=CBFB
acute myelogenous leukemia t 8 21 q22 q22 diagnosis 2005:2010[pubdate] *count=100 - BioMedLib™ search engine
MeSH-major] Core Binding Factor beta Subunit / genetics. Gene Dosage. Gene Duplication. Sarcoma, Myeloid / diagnosis. Sarcoma, ... Chemical-registry-number] 0 / Core Binding Factor Alpha 2 Subunit; 0 / Core Binding Factors ... Chemical-registry-number] 0 / CBFB protein, human; 0 / Core Binding Factor beta Subunit ... Chemical-registry-number] 0 / AML1-ETO fusion protein, human; 0 / Core Binding Factor Alpha 2 Subunit; 0 / DNA-Binding Proteins ...http://www.bmlsearch.com/?kwr=acute+myelogenous+leukemia+t+8+21+q22+q22+diagnosis+2005:2010%5Bpubdate%5D&cxts=100&stmp=b0
Calcium Amino Acid Chelate (Bottle Package)
Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (CBFA2T3), transcript variant 2 ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4 ... Adenovirus with ORF of interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40 ...http://trademetro.net/catalog/Medical-Health-&-Beauty-12.html?catid=12&order=catname&way=1
ZFIN Gene: cbfb
core-binding factor subunit beta Gene Symbol:. cbfb Sequence Ontology ID :. SO:0000704. ...http://zfin.org/ZDB-GENE-980526-440
ZFIN Publication: Blake et al., 2000
Core Binding Factor alpha Subunits. *Core Binding Factor beta Subunit. *DNA/metabolism ... Mammalian CBFB encodes a transcription factor (CBFbeta) that in combination with CBFalpha2 binds to specific DNA sequences and ... In biochemical analyses, cbfbeta binds to human CBFalpha2 and enhances its DNA binding. During zebrafish development, cbfb is ... expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors ...http://zfin.org/ZDB-PUB-001221-16
iFluor 750 streptavidin conjugate - Gentaur.com
PE Linked Polyclonal Antibody to Core Binding Factor Beta Subunit CBFb. Supplier:MyBioSource ... small amounts may get caught on the vials walls and/or lid due to shipping and handling factors. We recommend you to briefly ...https://gentaur.com/prod/ifluor-750-streptavidin-conjugate/aat/3396062421
CbfbF/F CD4-cre mouse cells and iT reg cells generated | Open-i
Inactivation of the gene encoding RUNX cofactor core-binding factor-beta (CBFbeta) in mice and small interfering RNA (siRNA)- ... Core Binding Factor Alpha 2 Subunit/genetics/immunology*. *Core Binding Factor Alpha 3 Subunit/genetics/immunology* ... Inactivation of the gene encoding RUNX cofactor core-binding factor-beta (CBFbeta) in mice and small interfering RNA (siRNA)- ... we show that the transforming growth factor-beta (TGF-beta) induces the expression of the Runt-related transcription factors ...https://openi.nlm.nih.gov/detailedresult.php?img=PMC2806624_JEM_20090596_GS_Fig6&req=4
core-binding factor, beta subunit. CCT4. 10575. CCT4. chaperonin containing TCP1, subunit 4 (delta). ... TFT: transcription factor targets C4: computational gene sets CGN: cancer gene neighborhoods CM: cancer modules C5: GO gene ...http://software.broadinstitute.org/gsea/msigdb/cards/GCM_APEX1.html
Translating Genomics to the Clinic: Implications of Cancer Heterogeneity | Clinical Chemistry
core-binding factor, beta subunit; RUNX1, runt-related transcription factor 1; MAGI3, membrane associated guanylate kinase, WW ... core-binding factor, beta subunit) gene, deletions in its partner RUNX1 (runt-related transcription factor 1), and fusion of ... CREB-binding protein; WNT, wingless-type MMTV integration site family; BRAF, v-raf murine sarcoma viral oncogene homolog B1.. ... The major factor of tumor heterogeneity has not always been addressed adequately, however, and that has important implications ...http://clinchem.aaccjnls.org/content/59/1/127.long
Tumor suppressor may actually fuel aggressive leukemia - Oncology Nurse Advisor
The researchers genetically inhibited both RUNX1 and an associated protein called core-binding factor subunit beta (Cbfb). By ... Scientists targeted a protein that is a transcription factor known as RUNX1, which also plays an important role in helping ...https://www.oncologynurseadvisor.com/home/headlines/web-exclusives/tumor-suppressor-may-actually-fuel-aggressive-leukemia/
Tumor suppressor may actually fuel aggressive leukemia - ONA
The researchers genetically inhibited both RUNX1 and an associated protein called core-binding factor subunit beta (Cbfb). By ... Scientists targeted a protein that is a transcription factor known as RUNX1, which also plays an important role in helping ...http://www.oncologynurseadvisor.com/web-exclusives/tumor-suppressor-may-actually-fuel-aggressive-leukemia/article/311871/
Molecular Vision: Effect of PITX2 knockdown on transcriptome of primary human trabecular meshwork cell cultures
The five genes identified by all the protocols were DIRAS3, CXCL6, SAMD5, CBFB (core-binding factor, beta subunit), and MEIS2. ... core-binding factor, beta subunit), and MEIS2 (meis homeobox 2). Real time PCR experiments verified results on a subset of ... latent transforming growth factor beta binding protein 2), a primary glaucoma causing gene, was not probed on the arrays . ... Loss of function mutations in the gene encoding latent transforming growth factor beta binding protein 2, LTBP2, cause primary ...http://www.molvis.org/molvis/v17/a136/
The genomic map of breast cancer: which roads lead to better targeted therapies? | Breast Cancer Research | Full Text
CBFB, encoding core-binding factor beta subunit, was mutated in four ER+ tumors. CBFB is a heterodimeric partner with the RUNX ... These fusions retained the kinase domain of AKT3 while disrupting the pleckstrin homology and PTEN-binding domains of AKT3 and ... This suggests that the mechanisms and environmental factors contributing to the development and progression of breast tumors ... the relatively small region of tumor in a core biopsy may still miss a significant percentage of the clonal lesions present in ...https://breast-cancer-research.biomedcentral.com/articles/10.1186/bcr3435
"The transcription factor PlagL2 activates Mpl transcription and signal" by Sean F. Landrette, Dmitri Madera et al.
We have previously shown that the transcription factor PLAGL2 promotes proliferation and cooperates with the leukemia fusion ... RNA-Binding Proteins; Receptors, Thrombopoietin; Oncogene Proteins, Fusion; Core Binding Factor beta Subunit; Gene Expression ... Leukemia, Myeloid, Acute; DNA-Binding Proteins; Transcription Factors; ... The transcription factor PlagL2 activates Mpl transcription and signaling in hematopoietic progenitor and leukemia cells ...https://escholarship.umassmed.edu/pgfe_pp/150/
- CBFB enhances DNA binding by RUNX1. (nih.gov)
- uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a fluorescence resonance energy transfer (FRET) assay. (nih.gov)
- Confirmation of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a time resolved fluorescence resonance energy transfer (TR-FRET) assay. (nih.gov)
- CBFB (Core-Binding Factor Beta Subunit) is a Protein Coding gene. (genecards.org)
- Antigen standard for core-binding factor, beta subunit (CBFB), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
- Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. (trademetro.net)
- Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. (wikipedia.org)
- Mammalian CBFB encodes a transcription factor (CBFbeta) that in combination with CBFalpha2 binds to specific DNA sequences and regulates expression of a number of hematopoietic genes. (zfin.org)
- We have also analyzed the expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors to cbfb in zebrafish hematopoiesis. (zfin.org)
- The researchers genetically inhibited both RUNX1 and an associated protein called core-binding factor subunit beta (Cbfb). (oncologynurseadvisor.com)
- The five genes were DIRAS3 (DIRAS family, GTP-binding RAS-like 3), CXCL6 (chemokine (C-X-C motif) ligand 6), SAMD5 (sterile alpha motif domain containing 5), CBFB (core-binding factor, beta subunit), and MEIS2 (meis homeobox 2). (molvis.org)
- Here, we present the crystal structures of the AML1/Runx-1/CBFalpha(Runt domain)-CBFbeta(core domain)-C/EBPbeta(bZip)-DNA, AML1/Runx-1/CBFalpha(Runt domain)-C/EBPbeta(bZip)-DNA, and AML1/Runx-1/CBFalpha(Runt domain)-DNA complexes. (rcsb.org)
- The hydrogen bonding network formed among CBFalpha(Runt domain) and CBFbeta, and CBFalpha(Runt domain) and DNA revealed the allosteric regulation mechanism of CBFalpha(Runt domain)-DNA binding by CBFbeta. (rcsb.org)
- In biochemical analyses, cbfbeta binds to human CBFalpha2 and enhances its DNA binding. (zfin.org)
- Inactivation of the gene encoding RUNX cofactor core-binding factor-beta (CBFbeta) in mice and small interfering RNA (siRNA)-mediated suppression of RUNX1 and RUNX3 in human T cells resulted in reduced expression of Foxp3. (nih.gov)
- Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. (dartmouth.edu)
- Circulating human CD4(+) CD25(high) CD127(-) T reg cells significantly expressed higher levels of RUNX3, FOXP3, and TGF-beta mRNA compared with CD4(+)CD25(-) cells.Furthermore, FOXP3 and RUNX3 were colocalized in human tonsil T reg cells.These data demonstrate Runx transcription factors as a molecular link in TGF-beta-induced Foxp3 expression in iT reg cell differentiation and function. (nih.gov)
- These data demonstrate Runx transcription factors as a molecular link in TGF-beta-induced Foxp3 expression in iT reg cell differentiation and function. (nih.gov)
- They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. (dartmouth.edu)
- Definitive evidence that alterations in chromosomal subunits play a role in cancer was obtained from studies of genes that control cancer growth, and discoveries of the transforming capacity of retroviruses in the 1970s established that virtually all cancers arise from the disruption of genetic material. (aaccjnls.org)
- To identify genes whose expressions in primary human trabecular meshwork (TM) cell cultures are affected by the transcription factor pituitary homeobox 2 (PITX2) and to identify genes that may have roles in glaucoma. (molvis.org)