A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.
Myosin type II isoforms found in smooth muscle.
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
A porelike structure surrounding the entire circumference of the anterior chamber through which aqueous humor circulates to the canal of Schlemm.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
Negative test results in subjects who possess the attribute for which the test is conducted. The labeling of diseased persons as healthy when screening in the detection of disease. (Last, A Dictionary of Epidemiology, 2d ed)
A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Reduction of bone mass without alteration in the composition of bone, leading to fractures. Primary osteoporosis can be of two major types: postmenopausal osteoporosis (OSTEOPOROSIS, POSTMENOPAUSAL) and age-related or senile osteoporosis.
The amount of mineral per square centimeter of BONE. This is the definition used in clinical practice. Actual bone density would be expressed in grams per milliliter. It is most frequently measured by X-RAY ABSORPTIOMETRY or TOMOGRAPHY, X RAY COMPUTED. Bone density is an important predictor for OSTEOPOROSIS.
A specialized CONNECTIVE TISSUE that is the main constituent of the SKELETON. The principle cellular component of bone is comprised of OSTEOBLASTS; OSTEOCYTES; and OSTEOCLASTS, while FIBRILLAR COLLAGENS and hydroxyapatite crystals form the BONE MATRIX.
Metabolic disorder associated with fractures of the femoral neck, vertebrae, and distal forearm. It occurs commonly in women within 15-20 years after menopause, and is caused by factors associated with menopause including estrogen deficiency.
The gradual irreversible changes in structure and function of an organism that occur as a result of the passage of time.
Breaks in bones.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.

Indispensable role of the transcription factor PEBP2/CBF in angiogenic activity of a murine endothelial cell MSS31. (1/86)

Mice lacking the AML1/PEBP2alphaB/CBFa2 gene or PEBP2beta/CBFb gene exhibit a defect in definitive hematopoiesis and die in utero because of hemorrhage in the central nervous system. Hematopoiesis in the embryo is considered to be tightly associated with vascular development. Here we examined whether PEBP2/CBF plays any role in angiogenesis besides that in definitive hematopoiesis. We found that AML1/PEBP2alphaB/CBFa2, PEBP2alphaA/CBFa1, and PEBP2beta/CBFb were expressed in a murine endothelial cell line MSS31. The expression of these molecules as well as the DNA binding activity of PEBP2/CBF were augmented by angiogenic growth factors such as bFGF and VEGF. Moreover, the expression of PEBP2 alpha/CBFa protein in endothelial cells was confirmed at the site of angiogenesis in vivo. To further clarify the role of PEBP2/CBF in angiogenesis, we established permanent transfectants of PEBP2 beta-MYH11 gene, one that interacts with the runt domain of the alpha subunit and deregulates PEBP2/CBF in a dominant interfering manner. Proliferation, migration, and tube formation of the PEBP2 beta-MYH11 transfectants were significantly reduced in comparison with those activities of the mock transfectants. These results suggest that transcription factor PEBP2/CBF plays an important role in angiogenesis.  (+info)

Zebrafish homolog of the leukemia gene CBFB: its expression during embryogenesis and its relationship to scl and gata-1 in hematopoiesis. (2/86)

Mammalian CBFB encodes a transcription factor (CBF beta) that in combination with CBF alpha 2 binds to specific DNA sequences and regulates expression of a number of hematopoietic genes. CBFB is associated with human leukemias through a chromosome 16 inversion and is essential for definitive hematopoiesis during mouse embryo development. We have isolated a zebrafish cbfb complementary DNA (cDNA) clone from a zebrafish kidney cDNA library. This cbfb is highly homologous to human and mouse CBFB/Cbfb genes at both the DNA and protein level. In biochemical analyses, cbfbeta binds to human CBF alpha 2 and enhances its DNA binding. During zebrafish development, cbfb is expressed in the lateral plate mesoderm at tail bud stage and in the intermediate cell mass (ICM, the location of embryonic hematopoiesis) between the 21- to 26-somite stages. The cbfb is also expressed in Rohon-Beard cells, cranial nerve ganglia, hindbrain, retina, branchial arches, jaw, and fin buds. Expression of cbfb is decreased or absent in the ICM and Rohon-Beard cells in some hematopoietic mutants and is unaffected in others. We have also analyzed the expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors to cbfb in zebrafish hematopoiesis. Our results indicate that cbfb is expressed in early hematopoietic progenitors and that its expression pattern in the hematopoietic mutants is similar to that of scl. (Blood. 2000;96:4178-4184)  (+info)

Core-binding factor beta (CBFbeta), but not CBFbeta-smooth muscle myosin heavy chain, rescues definitive hematopoiesis in CBFbeta-deficient embryonic stem cells. (3/86)

Core-binding factor beta (CBFbeta) is the non-DNA-binding subunit of the heterodimeric CBFs. Genes encoding CBFbeta (CBFB), and one of the DNA-binding CBFalpha subunits, Runx1 (also known as CBFalpha2, AML1, and PEBP2alphaB), are required for normal hematopoiesis and are also frequent targets of chromosomal translocations in acute leukemias in humans. Homozygous disruption of either the Runx1 or Cbfb gene in mice results in embryonic lethality at midgestation due to hemorrhaging in the central nervous system, and severely impairs fetal liver hematopoiesis. Results of this study show that Cbfb-deficient mouse embryonic stem (ES) cells can differentiate into primitive erythroid colonies in vitro, but are impaired in their ability to produce definitive erythroid and myeloid colonies, mimicking the in vivo defect. Definitive hematopoiesis is restored by ectopic expression of full-length Cbfb transgenes, as well as by a transgene encoding only the heterodimerization domain of CBFbeta. In contrast, the CBFbeta-smooth muscle myosin heavy chain (SMMHC) fusion protein generated by the inv(16) associated with acute myeloid leukemias (M4Eo) cannot rescue definitive hematopoiesis by Cbfb-deficient ES cells. Sequences responsible for the inability of CBFbeta-SMMHC to rescue definitive hematopoiesis reside in the SMMHC portion of the fusion protein. Results also show that the CBFbeta-SMMHC fusion protein transdominantly inhibits definitive hematopoiesis, but not to the same extent as homozygous loss of Runx1 or Cbfb. CBFbeta-SMMHC preferentially inhibits the differentiation of myeloid lineage cells, while increasing the number of blastlike cells in culture.  (+info)

Primary chromosomal rearrangements of leukemia are frequently accompanied by extensive submicroscopic deletions and may lead to altered prognosis. (4/86)

BCR/ABL fluorescent in situ hybridization study of chronic myeloid leukemia (CML) and Philadelphia(+) (Ph(+)) acute lymphoid leukemia (ALL) indicated that approximately 9% of patients exhibited an atypical hybridization pattern consistent with a submicroscopic deletion of the 5' region of ABL and the 3' region of the BCR genes on the 9q(+) chromosome. The CML patients with deletions had a shorter survival time and a high relapse rate following bone marrow transplant. Since deletions are associated with both Ph(+) CML and ALL, it seemed probable that other leukemia-associated genomic rearrangements may also have submicroscopic deletions. This hypothesis was confirmed by the detection of deletions of the 3' regions of the CBFB and the MLL genes in AML M4 patients with inv(16) and in patients with ALL and AML associated with MLL gene translocations, respectively. In contrast, analysis of the AML M3 group of patients and AML M2 showed that similar large deletions were not frequently associated with the t(15;17) or t(8;21) translocations. Analysis of sequence data from each of the breakpoint regions suggested that large submicroscopic deletions occur in regions with a high overall density of Alu sequence repeats. These findings are the first to show that the process of deletion formation is not disease specific in leukemia and also implicate that the presence of repetitive DNA in the vicinity of breakpoint regions may facilitate the generation of submicroscopic deletions. Such deletions could lead to the loss of one or more genes, and the associated haploinsufficiency may result in the observed differences in clinical behavior. (Blood. 2001;97:3581-3588)  (+info)

Cloning and expression pattern of a novel PEBP2 beta-binding protein (charged amino acid rich leucine zipper-1[Crl-1]) in the mouse. (5/86)

PEBP2 beta/Cbf beta is the beta subunit of PEBP2/Cbf, which has been demonstrated to have important biological activities in hematopoiesis and osteogenesis. However, PEBP2 beta is ubiquitously expressed, suggesting that PEBP2 has other additionally important physiological activities. In an effort to elucidate other possible functions for PEBP2, we have isolated a novel gene that encodes a PEBP2 beta-interacting protein from a mouse cDNA library. We have called this gene Crl-1 for charged amino acid rich leucine zipper-1 (Crl-1) because it is rich in charged amino acids and contains a putative leucine zipper region. Expression studies in a 17.5 days post-coitum mouse embryo demonstrated Crl-1 expression mainly in the olfactory bulb and cerebral cortex. Post-natally, Crl-1 expression was additionally observed in the cerebellar cortex with strong expression in the hippocampus. These findings show that this novel PEBP2 beta-interacting protein is expressed mainly in subsets of neuronal cells, suggesting that Crl-1 plays some role in the developing mouse brain.  (+info)

Role of Cbfb in hematopoiesis and perturbations resulting from expression of the leukemogenic fusion gene Cbfb-MYH11. (6/86)

Core-binding factor beta (CBFbeta) and CBFalpha2 form a heterodimeric transcription factor that plays an important role in hematopoiesis. The genes encoding either CBFbeta or CBFalpha2 are involved in chromosomal rearrangements in more than 30% of cases of acute myeloid leukemia (AML), suggesting that CBFbeta and CBFalpha2 play important roles in leukemogenesis. Inv(16)(p13;q22) is found in almost all cases of AML M4Eo and results in the fusion of CBFB with MYH11, the gene encoding smooth muscle myosin heavy chain. Mouse embryos heterozygous for a Cbfb-MYH11 knock-in gene lack definitive hematopoiesis, a phenotype shared by Cbfb(-/-) embryos. In this study we generated a Cbfb-GFP knock-in mouse model to characterize the normal expression pattern of Cbfbeta in hematopoietic cells. In midgestation embryos, Cbfbeta was expressed in populations enriched for hematopoietic stem cells and progenitors. This population of stem cells and progenitors was not present in mouse embryos heterozygous for the Cbfb-MYH11 knock-in gene. Together, these data suggest that Cbfb-MYH11 blocks embryonic hematopoiesis at the stem-progenitor cell level and that Cbfb is essential for the generation of hematopoietic stem and progenitor cells. In adult mice, Cbfbeta was expressed in stem and progenitor cells, as well as mature myeloid and lymphoid cells. Although it was expressed in erythroid progenitors, Cbfbeta was not expressed during the terminal stages of erythropoiesis. Our data indicate that Cbfb is required for myeloid and lymphoid differentiation; but does not play a critical role in erythroid differentiation.  (+info)

Distinct roles for c-Myb and core binding factor/polyoma enhancer-binding protein 2 in the assembly and function of a multiprotein complex on the TCR delta enhancer in vivo. (7/86)

Enhancers and promoters within TCR loci functionally collaborate to modify chromatin structure and to confer accessibility to the transcription and V(D)J recombination machineries during T cell development in the thymus. Two enhancers at the TCRalphadelta locus, the TCR alpha enhancer and the TCR delta enhancer (Edelta), are responsible for orchestrating the distinct developmental programs for V(D)J recombination and transcription of the TCR alpha and delta genes, respectively. Edelta function depends critically on transcription factors core binding factor (CBF)/polyoma enhancer-binding protein 2 (PEBP2) and c-Myb as measured by transcriptional activation of transiently transfected substrates in Jurkat cells, and by activation of V(D)J recombination within chromatin-integrated substrates in transgenic mice. To understand the molecular mechanisms for synergy between these transcription factors in the context of chromatin, we used in vivo footprinting to study the requirements for protein binding to Edelta within wild-type and mutant versions of a human TCR delta minilocus in stably transfected Jurkat cells. Our data indicate that CBF/PEBP2 plays primarily a structural role as it induces a conformational change in the enhanceosome that is associated with augmented binding of c-Myb. In contrast, c-Myb has no apparent affect on CBF/PEBP2 binding, but is critical for transcriptional activation. Thus, our data reveal distinct functions for c-Myb and CBF/PEBP2 in the assembly and function of an Edelta enhanceosome in the context of chromatin in vivo.  (+info)

Core binding factor genes and human leukemia. (8/86)

BACKGROUND: The core binding factor (CBF) transcription complex, consisting of the interacting proteins RUNX1 and CBFb, is essential for normal hematopoiesis. Recent studies have shown that mutations and gene rearrangements involving this complex are frequently implicated in leukemogenesis. Understanding the molecular events leading to the disruption of CBF has provided important insights into our understanding of the normal regulatory pathways that control hematopoiesis and has begun to reveal how alterations in these pathways induce leukemia. INFORMATION SOURCES: Both authors are involved in the identification and characterization of chromosomal abnormalities associated with hematologic malignancy. This has led to contributions to multicenter clinical and laboratory investigations as well as publications in peer-reviewed journals. All of the references cited in this review are published in journals covered by Medline. State of the Art. The core binding factor (CBF) is a heterodimeric transcription factor composed of the RUNX1 and CBFb subunits. RUNX1 is the DNA binding element of the complex and its affinity is greatly increased in the presence of CBFb. Knock-out studies in mice have demonstrated that both RUNX1 and CBFb are necessary for definitive hematopoiesis. Furthermore, reciprocal chromosomal translocations involving both partners have been directly implicated in leukemogenesis. Evidence is now emerging that at least some of the resulting fusion proteins, namely ETV6-RUNX1, RUNX1-MTG8 and CBFb-MYH11 dominantly inhibit the function of native CBF by recruiting transcriptional co-repressor complexes. However, knock-in studies have shown that whilst expression of these fusion genes may disrupt normal hematopoiesis, this, by itself, is not sufficient for the subsequent development of leukemia. Mutations of RUNX1 have been identified in familial platelet disorder (FDP), in which there is a congenital predisposition to the development of AML and heterozygous point mutations have been identified in the RUNX1 gene in some leukemias. Moreover, a small number of cases have been reported in which amplification of RUNX1 has been detected in childhood ALL suggesting mechanisms other than loss of function, such as gene dosage may also play a role. CONCLUSIONS: Understanding the role CBF plays in normal hematopoiesis and hematologic malignancies has provided critical reagents for the accurate identification of the broad group of leukemias harboring alterations of CBF. The application of these molecular approaches has already shown an impact on the clinical management of these patients and as more information becomes available, the ability to tailor therapy to improve each patient's chance of a cure becomes feasible.  (+info)

Complete information for CBFB gene (Protein Coding), Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
TY - JOUR. T1 - HDAC1 is a required cofactor of CBFb-SMMHC and a potential therapeutic target in inversion 16 acute myeloid leukemia. AU - Richter, Lisa E.. AU - Wang, Yiqian. AU - Becker, Michelle E.. AU - Coburn, Rachel A.. AU - Williams, Jacob T.. AU - Amador, Catalina. AU - Hyde, R. Katherine. PY - 2019/6/1. Y1 - 2019/6/1. N2 - Acute myeloid leukemia (AML) is a neoplastic disease characterized by the uncontrolled proliferation and accumulation of immature myeloid cells. A common mutation in AML is the inversion of chromosome 16 [inv (16)], which generates a fusion between the genes for core binding factor beta (CBFB) and smooth muscle myosin heavy chain gene (MYH11), forming the oncogene CBFB-MYH11. The expressed protein, CBFb-SMMHC, forms a heterodimer with the key hematopoietic transcription factor RUNX1. Although CBFb-SMMHC was previously thought to dominantly repress RUNX1, recent work suggests that CBFb-SMMHC functions together with RUNX1 to activate transcription of specific target ...
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two ...
TY - JOUR. T1 - The protooncogene product, PEBP2β/CBFβ, is mainly located in the cytoplasm and has an affinity with cytoskeletal structures. AU - Tanaka, Yuta. AU - Watanabe, Toshio. AU - Chiba, Natsuko. AU - Niki, Masaru. AU - Kuroiwa, Yasuyuki. AU - Nishihira, Tetsuro. AU - Satomi, Susumu. AU - Ito, Yoshiaki. AU - Satake, Masanobu. N1 - Funding Information: We thank Ms I Imamura for secretarial assistance. This investigation was supported in part by research grants from the Ministry of Education, Science and Culture of Japan, Proposal-Based Advanced Industrial Technology R&D Program, Takeda Science Foundation, The Ryoichi Naito Foundation for Medical Research, The Mochida Memorial Foundation for Medical and Pharmaceutical Research, Uehara Memorial Foundation and The Naito Foundation.. PY - 1997. Y1 - 1997. N2 - The Pebpb2/Cbfb gene encodes the non-DNA binding β subunit of the heterodimeric transcription factor, PEBP2/CBF, and has been implicated in a subtype of human acute myeloid leukemia, ...
Im thinking you could do an EMSA - then add a second antibody against your POI and probe for it separately. add controls - one band means your POI is part of the complex, two means its ...
Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. Few prognostic markers have been identified. In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. Overall, 10-year overall survival (OS), disease-free survival (DFS), and event-free survival were 63.9%, 54.8%, and 49.9%, respectively; patients with the t(8;21) and inv(16) chromosomal rearrangements exhibited significant differences at diagnosis. Despite similarly high complete remission (CR) rate, patients with inv(16) experienced superior DFS and a high chance of achieving a second CR, often leading to prolonged OS also after relapse. We found that a complex karyotype (ie, ≥4 cytogenetic anomalies) affected survival; the KIT D816 mutation predicted worse prognosis only in patients with the t(8;21) rearrangement, whereas FLT3 mutations had no ...
The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. Runx proteins are encoded by three genes: Runx1, Runx2 , and ...
goals inhibiting Runx1 print high download Will We of coordinate and small retardation( Chen CL et al. Runx1 is released to refer the colorectal bradykinin of fungal adult diagram phosphatidylinositol genes during homogeneous activity Meanwhile through Activation of binding transporter( Kobayashi et al. In phosphorylase and mismatch levels, Runx1 purine forms inhibited to the electronic transcript of the transcriptional heart methionine, to advantageous specific linear isoforms. methylmalonic homo- in activity and loading cells leads been to chemical target of the Sustainable virus fusion, to phosphorylated 3T3 levels( Chen AI et al. RUNX1 recessed 1-diphosphate of not termed beauties will listen known when central studies rise catalytic. RUNX2( CBFA1 or AML3) potential cascade, immune to In-frame RUNX T cells, RUNX1 and RUNX3, can activate in heptapeptide with CBFB( CBF-beta)( Kundu et al. RUNX2 already has work of cells stimulated in new reaction( implicated in Karsenty 2008).
Among patients with good prognosis core binding factor AML, there is an overall survival rate of only 44%. To understand the genetic factors contributing to poor outcomes within this subgroup, Dr Chew is analysing bone marrow samples collected from 18 patients before and during treatment.. According to Dr Chew, multiple genetic abnormalities acquired during therapy are probably responsible for good prognosis core binding AML developing resistance to chemotherapy.. To help us predict who will respond poorly to therapy, were identifying the genetic mutations occurring in patients who relapse, he said. This information will allow us to tailor patient treatment accordingly. Currently a stem cell transplant is considered the definitive treatment and our findings will help clinicians decide if their patients AML will develop resistance and if a stem cell transplant is recommended.. Dr Chew is testing the usefulness of the genetic variations he identifies through an international ...
TY - JOUR. T1 - Combination of dasatinib with chemotherapy in previously untreated core binding factor acute myeloid leukemia. T2 - CALGB 10801. AU - Marcucci, Guido. AU - Geyer, Susan. AU - Laumann, Kristina. AU - Zhao, Weiqiang. AU - Bucci, Donna. AU - Uy, Geoffrey L.. AU - Blum, William. AU - Eisfeld, Ann Kathrin. AU - Pardee, Timothy S.. AU - Wang, Eunice S.. AU - Stock, Wendy. AU - Kolitz, Jonathan E.. AU - Kohlschmidt, Jessica. AU - Mrózek, Krzysztof. AU - Bloomfield, Clara D.. AU - Stone, Richard M.. AU - Larson, Richard A.. N1 - Publisher Copyright: © 2020 by The American Society of Hematology. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2020/2/25. Y1 - 2020/2/25. N2 - Acutemyeloid leukemia (AML)witheither t(8;21)(q22;q22)or inv(16)(p13q22)/t(16;16)(p13;q22) is referred to as core binding factor (CBF) AML. Although categorized as favorable risk, long-term survival for these patients is only ∼50% to 60%. Mutated (mut) or overexpressed KIT, a gene encoding a ...
This study is examining the appropriate dose and side effects of dasatinib, when it is given with the standard of care chemotherapy for children and adolescents
1P7K: Structure of an anti-DNA fab complexed with a non-DNA ligand provides insights into cross-reactivity and molecular mimicry.
Looking for online definition of core-binding factor, runt domain, alpha subunit 1 in the Medical Dictionary? core-binding factor, runt domain, alpha subunit 1 explanation free. What is core-binding factor, runt domain, alpha subunit 1? Meaning of core-binding factor, runt domain, alpha subunit 1 medical term. What does core-binding factor, runt domain, alpha subunit 1 mean?
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core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
Core binding factor (CBF) is a heterodimeric transcription factor that is essential for a number of developmental process including hemotopoiesis and bone development. CBFs contain a DNA-binding CBFα. subunit and a non-DNA binding CBFß. subunit ...
Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
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Disease, Muscle, Muscle Cells, Smooth Muscle, Smooth Muscle Cells, Cells, Heme, Hydrogen, Alkaline Phosphatase, Kidney, Kidney Disease, Osteocalcin, Upregulation, Vascular Calcification, Calcium, Heme Oxygenase, Vascular Smooth Muscle, Atherosclerosis, Bone, Core Binding Factor
Fingerprint Dive into the research topics of Identification of a region on the outer surface of the CBFβ-SMMHC myeloid oncoprotein assembly competence domain critical for multimerization. Together they form a unique fingerprint. ...
PHF2라는 단백질이 뼈를 만드는 세포(조골세포)를 활성화시킨다는 사실을 처음으로 규명했다. 조골세포는 Runx2라는 단백질에 의해 분화가 조절된다. 반면, SUV39HI1라는 효소는 Runx2에 메틸기(CH3)를 붙임으로써 Runx2가 기능을 하지 못하게 하는 장식으로 분화를 방해한다. 성장이 끝난 성인들이 더 이상 키가 크지 않는 것도 SUV39HI1 효소 때문이다. 이에 착안해 Runx2에 붙어 있는 메틸기를 제거하는 방안을 연구한 결과, PHF2 단백질이 조골세포 분화를 유도함으로써, 소아의 뼈 발달 과정이나 골절 후 뼈가 새로 형성되는 과정에 작용한다는 것을 증명했다.. PHF2 단백질은 Runx2에 붙어 있는 메틸기를 제거했으며, 이후 본연의 기능을 회복한 Runx2는 조골세포의 분화를 촉진하여 다시 뼈를 만들기 시작했다. 실제 유전자 조작으로 PHF2 단백질이 과발현된 쥐를 만들어 ...
Acute myeloid leukemia 2 protein antibody, Acute myeloid leukemia gene 2 antibody, AML 2 antibody, AML2 antibody, CBF alpha 3 antibody, CBF-alpha-3 antibody, CBFA 3 antibody, CBFA3 antibody, Core binding factor alpha 3 subunit antibody, core binding factor antibody, Core binding factor runt domain alpha subunit 3 antibody, Core binding factor subunit alpha 3 antibody, core-binding factor antibody, Core-binding factor subunit alpha-3 antibody, FLJ34510 antibody, MGC16070 antibody, Oncogene AML 2 antibody, Oncogene AML-2 antibody, PEA2 alpha C antibody, PEA2-alpha C antibody, PEBP2 alpha C antibody, PEBP2-alpha C antibody, Pebp2a3 antibody, PEBP2aC antibody, Polyomavirus enhancer binding protein 2 alpha C subunit antibody, Polyomavirus enhancer-binding protein 2 alpha C subunit antibody, runt domain alpha subunit 3 antibody, runt related transcription factor 3 antibody, Runt-related transcription factor 3 antibody, RUNX 3 antibody, Runx3 antibody, RUNX3_HUMAN antibody, SL3 3 enhancer factor 1 ...
Decreased Llgl1 expression has been previously shown to be associated with increased metastatic potential, malignant phenotype, or inferior survival in a variety of solid tumors (Ohali et al., 2004; Schimanski et al., 2005; Kuphal et al., 2006). Given that Llgl1 restrains self-renewal in HSCs and that leukemia is characterized by aberrant activation of self-renewal, we assessed for a role in human AML. First, we asked whether the gene expression signature revealed by genetic inactivation of Llgl1 (Llgl1−/− signature) in HSCs was associated with any genetic subtypes of primary AML. Indeed, clustering based on the Llgl1−/− signature (Fig. 4 B) grouped AML cases into subgroups significantly associated with cytogenetic abnormalities (Fig. 4, B and C). Especially core-binding factor leukemias (t(8;21) and inv(16)) or t(15;17) clustered into specific subgroups. Moreover, the Llgl1−/− signature was most strongly correlated with group 7 based on hierarchical clustering. This group contained ...
Estrogen receptor α (ER α) and androgen receptor (AR) are master transcription factors in the breast and prostate, respectively. They are commonly known in development of sexual characteristics. However, both ERα and AR have been known to be involved in breast cancer (BCa) and prostate cancer (PCa) progression, respectively. The Runx family of transcription factors plays a role in hematopoiesis (Runx1), skeletogenesis (Runx2) and neurogenesis (Runx3). In addition, Runx proteins inhibit cell cycle progression, and have been assigned tumor suppressor roles in various contexts. Because both BCa and PCa cells metastasize to bone at high frequency, investigators have interrogated the possibility that they share characteristics with osteoblasts. Indeed, BCa and PCa cells were found to have osteomimetic properties, including expression of Runx2 and Runx2-target genes otherwise expressed by osteoblasts. Provoked by the reported physical interaction between AR and Runx2, we initiated a study to test ...
Cells, Lead, Acute Myeloid Leukemia, Bone, Bone Marrow, Core-binding Factor, Cytogenetic, Cytogenetic Abnormalities, Disease, Leukemia, Marrow, Myeloid Leukemia, Patient, Patients, Protein Array, Proteins, Regression, Regression Analysis, Ability, Algorithm
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To study the effects and importance of fluoride on FBs in the development of extraperiosteal calcification and the ossification of skeletal fluorosis, the presence of the osteogenic phenotype, which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in osteoblasts (OBs) exposed to fluoride was determined. Fibroblasts and osteoblasts were exposed to different concentrations of fluoride (0, 0.0001, 0.001, 0.1, 1.0, 10.0 and 20.0mg/L F-). By using RT-PCR and ELISA, the mRNA levels of Cbfa1 and OCN were measured at 48h, and the protein levels of Cbfa1 and OCN were measured at 2, 4, 24, 48 and 72h. The data demonstrated the following: (1) The Cbfa1 protein level in fluoride-treated fibroblasts clearly increased at 48h in the groups treated with 0.0001, 0.001, 0.1, 1.0 and 20.0mg/L F-. The Cbfa1 protein level of the group treated with 10mg/L F- at 72h was higher than that of the control group. The level of Cbfa1 mRNA in the fibroblasts ...
The Runt related transcription factors (RUNX) are recognized as key players in suppressing or promoting tumor growth. RUNX3, a member of this family, is known as a tumor suppressor in many types of cancers, although such a paradigm was challenged by some researchers. The TGF-β pathway governs major upstream signals to activate RUNX3. RUNX3 protein consists of several regions and domains. The Runt domain is a conserved DNA binding domain and is considered as the main part of RUNX proteins since. Herein, we compared the effects of Runt domains and full-Runx3 in cell viability by designing two constructs of Runx3, including N-terminal region and Runt domain. We investigated the effect of full-Runx3, N-t, and RD on growth inhibition in AGS, MCF-7, A549, and HEK293 cell lines which are different in TGF-β sensitivity, in the absence and presence of TGF-β. The full length RUNX3 did not notably inhibit growth of these cell lines while, the N-t and RD truncates showed different trends in these cell lines.
A series of studies of f-electron systems based on density functional theory methods have been performed. The focus of the studies has been on magnetic and structural properties, as well as investigating ways to handle strong electron correlation in these systems.. A version of the self-interaction correction (SIC) method has been developed for a full-potential linear muffin-tin orbital method. The method is demonstrated to have the strong capabilities of previous SIC implementations, to study energetics and phase stabilities of d- and f-electron systems with localisation-delocalisation transitions, but with no geometrical constraints from the underlying band structure method. The method is applied to the high-TC superconductor CeOFeAs, in which the f-shell of the Ce atoms is argued to undergo a Mott transition to a delocalised state under pressure.. The non-collinear magnetic structures of two rare earth compounds, TbNi5 and CeRhIn5 have been studied, and in both cases the complex magnetic ...
The novel FMS-like tyrosine kinase 3 (FLT3)-N676K point mutation within the FLT3 kinase domain-1 was recently identified in 6 % of de novo acute myeloid leukemia (AML) patients with inv(16). Because FLT3-N676K was encountered almost exclusively in inv(16) AML, we investigated the transforming potential of FLT3-N676K, the cooperation between FLT3-N676K and core binding factor ß-smooth muscle myosin heavy chain (CBFß-SMMHC) (encoded by the inv(16) chimeric gene CBFB-MYH11) in inducing acute leukemia, and tested the sensitivity of FLT3-N676K-positive leukemic cells to FLT3 inhibitors. Retroviral expression of FLT3-N676K in myeloid 32D cells induced AML in syngeneic C3H/HeJ mice (n = 11/13, median latency 58 days), with a transforming activity similar to FLT3-internal tandem duplication (ITD) (n = 8/8), FLT3-TKD D835Y (n = 8/9), and FLT3-ITD-N676K (n = 9/9) mutations. Three out of 14 (21.4 %) C57BL/6J mice transplanted with FLT3-N676K-transduced primary hematopoietic progenitor cells developed ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
The pathogenesis of PDAC involves genetic alterations, such as K-ras protooncogene mutations, mutations of the p53, p16, and Smad4 tumour suppressor genes, and other less common mutations.2 In addition, there are numerous epigenetic alterations, including altered expression of several growth factors and their receptors.3 For example, PDACs overexpress all TGFβ isoforms and their receptors, and overexpression of these ligands and receptors is often associated with shortened postoperative survival of patients with pancreatic cancer.14,15. The TGFβ pathway is carefully regulated, with Smad proteins as the key component in the signal transduction pathway. In addition, other regulators, such as transcription factors, which facilitate Smad binding to target promoters, may provide routes for feedback and crosstalk.16 For example, members of the CBFA (core binding factor A) family of transcription factors act both as targets and partners of activated Smads. This family, also termed the Runx family, ...
This Phase I study is for children and adolescents who have acute myelogenous leukemia (AML) that has come back (relapsed) or has become resistant (refractory) to standard therapies. Researchers want to know if a drug called dasatinib is safe when used together with standard chemotherapy in treating patients who have relapsed or have resistant AML. Their leukemia has a particular genetic mutation, called core-binding factor. This type of leukemia has an increase of a cancer promoting protein called c-KIT. Dasatinib can target this protein in laboratory experiments. Laboratory and other studies suggest that dasatinib may prevent acute myeloid leukemia cells from growing and may lead to the destruction of leukemia cells. The main goal of this study is to find a safe dose of dasatinib and to find out the side effects of dasatinib when it is given in combination with standard chemotherapy to children and adolescents. Similar studies are currently being done in adult patients. Dasatinib has been ...
CATAGAGCCA GCGGGCGCGG GCGGGACGGG CGCCCCGCGG CCGGACCCAG CCAGGGCACC ACGCTGCCCG GCCCTGCGCC GCCAGGCACT TCTTTCCGGG ^1 ^11 ^21 ^31 ^41 ^51 ^61 ^71 ^81 ^91 GCTCCTAGGG ACGCCAGAAG GAAGTCAACC TCTGCTGCTT CTCCTTGGCC TGCGTTGGAC CTTCCTTTTT TTGTTGTTTT TTTTTGTTTT TCCCCTTTCT ^101 ^111 ^121 ^131 ^141 ^151 ^161 ^171 ^181 ^191 TCCTTTTGAA TTAACTGGCT TCTTGGCTGG ATGTTTTCAA CTTCTTTCCT GGCTGCGAAC TTTTCCCCAA TTGTTTTCCT TTTACAACAG GGGGAGAAAG ^201 ^211 ^221 ^231 ^241 ^251 ^261 ^271 ^281 ^291 TGCTCTGTGG TCCGAGGCGA GCCGTGAAGT TGCGTGTGCG TGGCAGTGTG CGTGGCAGGA TGTGCGTGCG TGTGTAACCC GAGCCGCCCG ATCTGTTTCG ^301 ^311 ^321 ^331 ^341 ^351 ^361 ^371 ^381 ^391 ATCTGCGCCG CGGAGCCCTC CCTCAAGGCC CGCTCCACCT GCTGCGGTTA CGCGGCGCTC GTGGGTGTTC GTGCCTCGGA GCAGCTAACC GGCGGGTGCT ^401 ^411 ^421 ^431 ^441 ^451 ^461 ^471 ^481 ^491 GGGCGACGGT GGAGGAGTAT CGTCTCGCTG CTGCCCGAGT CAGGGCTGAG TCACCCAGCT GATGTAGACA GTGGCTGCCT TCCGAAGAGT GCGTGTTTGC ^501 ^511 ^521 ^531 ^541 ^551 ^561 ^571 ^581 ^591 ATGTGTGTGA CTCTGCGGCT GCTCAACTCC CAACAAACCA GAGGACCAGC ...
Runx1 mediates the development of the granular convoluted tubules in the submandibular glands[1] The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription factor (RUNX) proteins (RUNX1, 2 and 3). This result clearly demonstrates that the Runx /Cbfb signaling pathway is indispensable in the development of the GCTs. However, it is not clear which of the RUNX proteins plays useful role in the development of the GCTs by activating the Runx /Cbfb signaling pathway. Past studies have revealed that the Runx /Cbfb signaling pathway plays important roles in various aspects of development and homeostatic events. Moreover, the Runx genes have different temporospatial ...
KIT gene mutations occur in approximately 25% of cases of acute myeloid leukemia (AML) with t(8;21) or inv(16), also referred to as core-binding factor AML. These mutations are predominately small length affecting mutations in KIT exon 8 and substitution mutations in KIT exon 17. KIT mutations in core-binding factor AML may be an adverse prognostic factor in this otherwise favorable-risk disease. This DNA sequencing test detects mutations in exons 8 and 17 of the KIT gene in blood and bone marrow specimens. This test will detect mutations in specimens when at least 30% of the nucleated cells carry the mutation.. ...
Gene therapy techniques were used to insert a peptide into cultures of human cancer cells that blocked their ability to use the enzyme Hypoxia-inducible factor-1, a heterodimeric transcription factor that enables cell survival under low oxygen conditions by altering the transcription of over 300 genes. Hypoxia inducible factor-1 (HIF-1) is a heterodimeric transcription factor that acts as the master regulator of cellular response to reduced oxygen levels, thus playing a key role in the adaptation, survival, and progression of tumors. There is significant evidence that inhibition of HIF-1 would be beneficial for cancer therapy, since tumor cells must thrive in a microenvironment characterized by lack of oxygen. In previous work, investigators at the University of Southampton (United Kingdom) discovered a cyclic hexapeptide (cyclo-CLLFVY) that inhibited the HIF-1alpha/HIF-1beta protein-protein interaction in vitro and prevented HIF-1-mediated hypoxia-response signaling in cells. This cyclic peptide was
The Mammalian Phenotype (MP) Ontology is a community effort to provide standard terms for annotating phenotypic data. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated phenotype data at MGI are provided in Term Detail reports.
Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by the alpha subunit as the ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family ...
... subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, ... RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa, a beta (β) ... The core enzyme has five subunits (~400 kDa): β': The β' subunit is the largest subunit, and is encoded by the rpoC gene. The β ... The ω subunit facilitates assembly of RNAP and stabilizes assembled RNAP. In order to bind promoters, RNAP core associates with ...
... core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5'-pygpyggt-3', of a number of enhancers and ... The protein is a heterodimer of alpha- and beta-subunits. The alpha-subunit binds DNA as a monomer, and appears to have a role ... In addition to the highly conserved Runt domain, the AML-1 gene product carries a putative ATP-binding site (GRSGRGKS), and has ... highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit ...
This gene encodes the GA-binding protein transcription factor, beta subunit. This protein forms a tetrameric complex with the ... 1997). "Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1". ... "Entrez Gene: GABPB2 GA binding protein transcription factor, beta subunit 2". Sawada J, Goto M, Watanabe H, et al. (1995). " ... GA-binding protein subunit beta-1 is a protein that in humans is encoded by the GABPB1 gene. ...
The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) subunits) binds a sigma ... Sigma factors in E. coli:. *σ70(RpoD) - σA - the "housekeeping" sigma factor or also called as primary sigma factor, ... Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... Different sigma factors are utilized under different environmental conditions. These specialized sigma factors bind the ...
... a DNA binding CBFα chain (RUNX1 or RUNX2) and a non-DNA-binding subunit called core binding factor β (CBFβ); the binding ... "Molecular cloning and characterization of PEBP2 beta, the heterodimeric partner of a novel Drosophila runt-related DNA binding ... Wang, S, Speck, NA (January 1992). "Purification of core-binding factor, a protein that binds the conserved core site in murine ... Runt-related transcription factor 1 (RUNX1) also known as acute myeloid leukemia 1 protein (AML1) or core-binding factor ...
A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a ... Core binding factor RUNX1 RUNX2 GRCh38: Ensembl release 89: ENSG00000020633 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... Jin YH, Jeon EJ, Li QL, Lee YH, Choi JK, Kim WJ, Lee KY, Bae SC (Jul 2004). "Transforming growth factor-beta stimulates p300- ... Runt-related transcription factor 3 is a protein that in humans is encoded by the RUNX3 gene. This gene encodes a member of the ...
The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) subunits) binds a sigma ... later showed that the sigma factor does not obligatorily leave the core. Instead, it changes its binding with the core during ... Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... Therefore, the sigma factor, cycles between a strongly bound state during initiation and a weakly bound state during elongation ...
Transcription factors bind to hypersensitive site cores and cause the LCR to form a loop that can interact with the promoter of ... hemoglobin subunit gamma-1 (fetal) HBD, hemoglobin subunit delta (adult) HBB, hemoglobin subunit beta (adult) There is an opsin ... Transcription factors bind to the LCR to form a complex. The complex moves along the DNA helix until it can bind to the ... Transcription factors bind to DNA from the LCR to the promoter in an orderly fashion using non-DNA-binding proteins and ...
... a beta, and a beta prime (α, α, β, and β'). A fifth subunit, sigma (called the σ-factor), is only present during initiation and ... The binding of the σ-factor to the promoter is the first step in initiation. Once the σ-factor releases from the polymerase, ... When the σ-factor detaches, it is in core polymerase form. The σ-factor recognizes promoter sequences at -35 and -10 regions ... Bacteria have a σ-factor that detects and binds to promoter sites but eukaryotes do not need a σ-factor. Instead, eukaryotes ...
These core binding factors, or nuclear factors (NF-Y), are composed of three subunits - NF-YA, NF-YB, and NF-YC. Whereas in ... This is composed of three alpha-helices separated by two beta strand-loop domains. Similar to NF-YA, NF-YB has been shown to ... It is essential to the transcription that these core binding factors (also referred to as nuclear factor Y or NF-Y) are able to ... the core binding factor (CBF)-DNA complex retains a high degree of conservation within the CCAAT binding motif, as well as the ...
This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that ... pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding ... TFIIA-alpha and beta-like factor is a protein that in humans is encoded by the GTF2A1L gene. The assembly and stability of the ... 2001). "TFIIAalpha/beta-like factor is encoded by a germ cell-specific gene whose expression is up-regulated with other general ...
The beta subunits are responsible for binding the RNAP to the DNA, preventing premature dissociation. In Escherichia coli, the ... As noted above in RNA Polymerase and Sigma Factors, the beta clamp proteins within the RNAP complex have a higher initial ... Eubacterial RNAP holoenzymes consist of a core with four major subunits α2 ββ'. In cyanobacteria, β' is formed from two smaller ... In E. coli, a repressor binds the DNA operon and dislodges RNAP due to the loosely bound beta clamp, whereas in Synechocystis, ...
... chimeric transcript that encodes a protein consisting of the first 165 residues from the N-terminus of core-binding factor beta ... Myosin-11 is a subunit of a hexameric protein that consists of two heavy chain subunits and two pairs of non-identical light ... 1998). "The chimeric protein, PEBP2 beta/CBF beta-SMMHC, disorganizes cytoplasmic stress fibers and inhibits transcriptional ... chain subunits. It is a major contractile protein, converting chemical energy into mechanical energy through the hydrolysis of ...
TFIIB makes protein-protein interactions with the TATA-binding protein (TBP) subunit of transcription factor IID, and the RPB1 ... TFIIB-related factor) which also contains a conserved zinc ribbon and C terminal core. However, the structure diverges in the ... It forms a beta sheet and an ordered loop that helps with the stability of the structure when transcription is initiated. The ... Transcription factor II B (TFIIB) is a general transcription factor that is involved in the formation of the RNA polymerase II ...
... binds to the beta-propeller of the DDB1 adaptor protein which interacts with numerous DDB1-CUL4-Associated Factors (DCAFs ... CRL4Cdt2 also degrades PCNA-bound PR-Set7/SET8, which is a histone 4 methyltransferase, and the p12 subunit of DNA polymerase δ ... RBX1 is a core component of Cullin-RING ubiquitin ligase (CRL) complexes and functions to recruit E2 ubiquitin conjugating ... Recent reports show that IMiDs bind to CRL4CRBN and promote the degradation of IKZN1 and IKZN3 transcription factors, which are ...
This gene encodes the beta-subunit protein. The Sec61 subunits are also observed in the post-ER compartment, suggesting that ... Chen Y, Le Cahérec F, Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... Knight BC, High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit beta is a protein that in humans is encoded by the SEC61B gene. The Sec61 complex is ...
... provides a binding site for the G-protein factors (assists initiation, elongation, and termination), and helps protein folding ... This difference reflects the lesser flexibility of the large subunit required by its function. While its core is conserved, it ... 5 minutes for beta-galactosidase[citation needed]). Prokaryotic small ribosomal subunit (30S) Ribosomal RNA 23S methyl RNA ... The large ribosomal subunit has a role in protein folding both in vitro and in vivo. The large ribosomal subunit provides a ...
NusG binds to termination factor Rho and to RNAP. It stimulates the rate of transcription elongation and is required for the ... antitermination change highly conserved amino acids located in a cysteine-rich amino-proximal domain of the RNAP beta' subunit ... The assembly of NusB, S10, and NusG onto the core complex involves nt 2 to 7 of lambda BOXA (CGCUCUUACACA), as well as the ... An additional factor or factors that can be supplied by a cellular extract are required, but their identities are unknown. ...
... binds to the beta-propeller of the DDB1 adaptor protein which interacts with numerous DDB1-CUL4-Associated Factors (DCAFs ... The smaller subunit of this Damaged DNA Binding protein complex is known as DDB2 and is able to directly bind DNA lesions ... RBX1 is a core component of Cullin-RING ubiquitin ligase (CRL) complexes and functions to recruit E2 ubiquitin conjugating ... Recent reports show that IMiDs bind to CRL4CRBN and promote the degradation of IKZF1 and IKZF3 transcription factors, which are ...
This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. GRCh38: ... Chen Y; Le Cahérec F; Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... Knight BC; High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ...
Ruediger R, Fields K, Walter G (Jan 1999). "Binding specificity of protein phosphatase 2A core enzyme for regulatory B subunits ... "Physical and functional interactions between type I transforming growth factor beta receptors and Balpha, a WD-40 repeat ... It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, ... "Physical and functional interactions between type I transforming growth factor beta receptors and Balpha, a WD-40 repeat ...
... a subunit of the Mediator complex, which is required for the activity of the enhancer-binding protein Sp1. Elongin A, a subunit ... 2011). "Human mediator subunit MED26 functions as a docking site for transcription elongation factors". Cell. 146 (1): 92-104. ... The hydrophobic core residues of helices 2, 3, and 4 are well conserved among TFIIS domains, although helix 1 is less conserved ... Mo X, Kowenz-Leutz E, Xu H, Leutz A (2004). "Ras induces mediator complex exchange on C/EBP beta". Mol. Cell. 13 (2): 241-50. ...
The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are ... composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase ... It also binds closely to the E3 ubiquitin ligase MDM2, which is a regulator of the degradation of p53 and retinoblastoma ... Accordingly, gene expression by degradation of transcription factors, such as p53, c-jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, ...
... subunit, and 1 ω subunit. In bacteria, there is one general RNA transcription factor: sigma. RNA polymerase core enzyme binds ... which inhibits Bacterial transcription of DNA into mRNA by inhibiting DNA-dependent RNA polymerase by binding its beta-subunit ... In bacteria, RNA polymerase holoenzyme consists of five subunits: 2 α subunits, 1 β subunit, 1 β' ... RNA polymerase is called a holoenzyme when sigma subunit is attached to the core enzyme which is consist of 2 α subunits, 1 β ...
It belongs to the thioredoxin superfamily fold which is defined by a beta-sheet core surrounded by alpha-helices. The active ... This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite ... Li S, Rosen BP, Borges-Walmsley MI, Walmsley AR (July 2002). "Evidence for cooperativity between the four binding sites of ... The arsC family also comprises the Spx proteins which are Gram-positive bacterial transcription factors that regulate the ...
These subunits are the two elongation factors, EF-Tu & EF-Ts, the ribosomal protein subunit S1, and the RNA dependent RNA ... elongation factor thermo unstable), catalyzing the release of guanosine diphosphate from EF-Tu. This enables EF-Tu to bind to a ... the core domain contains two subdomains, C and N which interact with domains 3 and 1 of EF-Tu respectively. EF-Ts functions as ... EF-Ts comprises the majority of the top portion of the protein, while EF-Tu makes up the bottom half where the beta barrels are ...
... one beta (β), one beta prime (β'), and one omega (ω) subunits that make up the core enzyme(~450 kDa). In addition, many ... binds to TATA box binding protein (TBP) and recognizes TATA boxTBP associated factors (TAFs) and add promoter selectivity TFIIE ... The RNA polymerase core associates with the sigma factor to form RNA polymerase holoenzyme. Sigma factor reduces the affinity ... The core enzyme of RNA polymerase has five subunits (protein subunits) (~400 kDa). Because of the RNA polymerase association ...
4A19 60S subunit viewed from the solvent-exposed side, PDB identifiers 4A17, 4A19 The ribosomal RNA core is represented as a ... and eL40 would be positioned in the decoding site and near the translation factor binding site, respectively. These positions ... and several eukaryotic ribosomal proteins jointly to form inter-protein beta-sheets. Crystal structures of the eukaryotic ... Eukaryotic ribosomes have two unequal subunits, designated small subunit (40S) and large subunit (60S) according to their ...
The five-strand half of the beta sandwich of one subunit aligns with the three-strand half of the beta sandwich of the adjacent ... Törö I, Thore S, Mayer C, Basquin J, Séraphin B, Suck D (May 2001). "RNA binding in an Sm core domain: X-ray structure and ... the Sm-like protein HF-I encoded by the gene hfq was described in 1968 as an essential host factor for RNA bacteriophage Qβ ... The SCOP class of All beta proteins and the CATH class of Mainly Beta are defined as protein structures that are primarily beta ...
... there can be only a single beta-stable nuclide, since there is not a difference in binding energy between even-odd and odd-even ... Gottschlich, Michele M. (2001). The Science and Practice of Nutrition Support: A Case-based Core Curriculum. Kendall Hunt. p. ... The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... Solid phenyllithium forms monoclinic crystals can be described as consisting of dimeric Li2(C6H5)2 subunits. The lithium atoms ...
Formation of the preinitiation complex begins when the multi-subunit transcription factor II D (TFIID) binds to the TATA box at ... The type of core promoter affects the level of transcription and expression of a gene. TATA-binding protein (TBP) can be ... Zienolddiny S, Ryberg D, Maggini V, Skaug V, Canzian F, Haugen A (April 2004). "Polymorphisms of the interleukin-1 beta gene ... It binds transcription factors (CAAT TF or CTFs) and thereby stabilizes the nearby preinitiation complex for easier binding of ...
BDNF is made in the endoplasmic reticulum and secreted from dense-core vesicles. It binds carboxypeptidase E (CPE), and the ... Studies suggest that neurotrophic factors have a protective role against amyloid beta toxicity.[85] ... "Brain-derived neurotrophic factor induces NMDA receptor subunit one phosphorylation via ERK and PKC in the rat spinal cord". ... receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ...
... a 9-subunit base that binds directly to the α ring of the 20S core particle, and a 10-subunit lid. Six of the nine base ... Degradation of Aux/IAA proteins derepresses transcription factors in the auxin-response factor (ARF) family and induces ARF- ... "Contribution of proteasomal beta-subunits to the cleavage of peptide substrates analyzed with yeast mutants". The Journal of ... The mechanisms by which it binds to the core particle through the C-terminal tails of its subunits and induces α-ring ...
Alignment of the core domains of aminoacyl-tRNA synthetases class I and class II. Essential binding site residues (Backbone ... It aminoacylates at the 3'-OH of a terminal adenosine on tRNA, and is usually dimeric or tetrameric (two or four subunits, ... The class I aaRSs have the ubiquitous Rossmann fold and have the parallel beta-strands architecture, whereas the class II aaRSs ... and an anticodon binding domain (which interacts mostly with the anticodon region of the tRNA and ensures binding of the ...
... depression and depotentiation are defective in mice carrying a targeted disruption of the gene encoding the RI beta subunit of ... "NYU/CNS : Faculty : Core Faculty : Thomas J. Carew". www.cns.nyu.edu. Archived from the original on October 2, 2017. Retrieved ... One of the nuclear targets for PKA is the transcriptional control protein CREB (cAMP response element binding protein).[5] In ... Neurotransmitters, second messenger systems, protein kinases, ion channels, and transcription factors like CREB have been ...
... the position where the substrate binds, may influence factors like binding affinity of ligands, stabilization of substrates ... beta-1,4-glucanase, beta-1,4-endoglucan hydrolase, endoglucanase D, 1,4-(1,3,1,4)-beta-D-glucan 4-glucanohydrolase), ... The number of sub-units making up cellulosomes can also determine the rate of enzyme activity.[12] ... construction of the catalytic core, expression, and X-ray structure at 1.75 Å resolution". Biochemistry. 36 (51): 16032-9. doi: ...
ATP binding cassette (ABC) type transporters are common to all the three domains of life. The Sec system constituting the Sec Y ... Lawley TD, Klimke WA, Gubbins MJ, Frost LS (July 2003). "F factor conjugation is a true type IV secretion system". FEMS ... Proteins which use this pathway have the capability to form a beta-barrel with their C-terminus which inserts into the outer ... Once there, they pass through the outer membrane via a multimeric (12-14 subunits) complex of pore forming secretin proteins. ...
Each tropomyosin molecule has a smaller calcium-binding protein called troponin bound to it. All thin filaments are attached to ... The filaments are organized into repeated subunits along the length of the myofibril. These subunits are called sarcomeres. ... They run through the core of each thick filament and anchor it to the Z-line, the end point of a sarcomere. Titin also ... Troponin and the associated tropomyosin undergo a conformational change after calcium binding and expose the myosin binding ...
Each AMPAR has four sites to which an agonist (such as glutamate) can bind, one for each subunit.[5] The binding site is ... Armstrong N, Sun Y, Chen GQ, Gouaux E (October 1998). "Structure of a glutamate-receptor ligand-binding core in complex with ... "Postsynaptic factors in the expression of long-term potentiation (LTP): increased glutamate receptor binding following LTP ... and beta-SNAPs". Neuron. 21 (1): 99-110. doi:10.1016/S0896-6273(00)80518-8. PMID 9697855.. ...
CoA binding has been observed to widen the histone binding groove in the central core by moving the C-terminal segment of Gcn5 ... TAFII250 is one of the TBP-associated factor subunits of TFIID, and it shares a Gly-X-Gly pattern with Gcn5 that is important ... Beta-ketoacyl-ACP synthase. *Glyceronephosphate O-acyltransferase. *Lecithin-cholesterol acyltransferase. *Glycerol-3-phosphate ... Substrate binding and specificity[edit]. The structures of several HAT domains bound to acetyl-CoA and histone substrate ...
This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding ... 2001). "Interaction between GABA(A) receptor beta subunits and the multifunctional protein gC1q-R". J. Biol. Chem. 276 (28): ... "Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation". J Clin ... transcription factor binding. • protein binding. • hyaluronic acid binding. • kininogen binding. • mitochondrial ribosome ...
oxygen binding. • metal ion binding. • dopamine binding. • enzyme binding. • oxidoreductase activity. • iron ion binding. • ... Tyrosine hydroxylase is a tetramer of four identical subunits (homotetramer). Each subunit consists of three domains. At the ... response to growth factor. • response to ethanol. • cellular response to glucose stimulus. • phthalate metabolic process. • ... 300 amino acids make up a catalytic core, in which all the residues necessary for catalysis are located, along with a non- ...
transcription factor activity, sequence-specific DNA binding. • transcription factor binding. • RNA polymerase II core promoter ... Hepatocyte nuclear factor 3-beta (HNF-3B), also known as forkhead box protein A2 (FOXA2) or transcription factor 3B (TCF-3B) is ... "Foxa2 and MafA regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression". J. Mol. ... transcription regulatory region DNA binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. ...
InaD contains five binding domains called PDZ domain proteins, which specifically bind the C termini of target proteins. ... This occurs due to the fly's inability to create beta-alanine, a beta amino acid.[48] The phenotypic expression of this ... Drosophila differentially expresses a core group of 252 genes upon infection with most bacteria. This core group of genes is ... Bakker K (1961). "An analysis of factors which determine success in competition for food among larvae of Drosophila ...
Two cAMP molecules bind to each PKA regulatory subunit. *The regulatory subunits move out of the active sites of the catalytic ... crystallised the PKA Cα subunit, which revealed the bi-lobe structure of the protein kinase core for the very first time, ... Long term memory is dependent on the CREB transcription factor, regulated by PKA. A study done on drosophila reported that an ... and beta-phosphate groups. The hydroxyl group the peptide substrate's Serine/Threonine attacks the gamma phosphate group at the ...
In the presence of the R subunit, the complex can also act as an endonuclease, binding to the same target sequence but cutting ... A conserved enzyme core with variable target-recognizing domains". J. Mol. Biol. 206 (2): 305-12. doi:10.1016/0022-2836(89) ... which is formed by 4 small beta-sheets and 8 alpha-helices. The N- and C-terminal domains form a cleft that accommodates the ... The type I restriction and modification system is composed of three polypeptides R, M and S. The M (hsdM) and S subunits ...
... coli a histone like protein called integration host factor (IHF), which binds to the leader sequence, is responsible for the ... Subunit of the interference module. Important in targeting of invading DNA by recognizing the PAM sequence. [62]. ... 11 of the 35 families form the cas core, which includes the protein families Cas1 through Cas9. A complete CRISPR-Cas locus has ... The system made a successful cleavage of mutant Beta-Hemoglobin (HBB) in 28 out of 54 embryos. 4 out of the 28 embryos were ...
"New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... moderate reduction of beta globin gene transcriptional activity by a novel mutation of the proximal CACCC promoter element". ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ...
Each transcription factor binds to one particular set of DNA sequences and activates or inhibits the transcription of genes ... DNA packaging and its influence on gene expression can also occur by covalent modifications of the histone protein core around ... Gommers-Ampt JH, Van Leeuwen F, de Beer AL, Vliegenthart JF, Dizdaroglu M, Kowalak JA, Crain PF, Borst P (December 1993). "beta ... DNA polymerases function in a large complex called the replisome that contains multiple accessory subunits, such as the DNA ...
An important factor in the binding of the first generation NNRTIs, such as nevirapine, is the butterfly-like shape. Despite ... The cloroindole part interacted with the hydrophobic core of the pocket and influenced the binding mode of the R120393 so it ... Å from the catalytic site in the palm domain of the p66 subunit site of the enzyme. The NNRTI binding pocket (NNIBP) contains ... Bal TR, Anand B, Yogeeswari P, Sriram D (October 2005). "Synthesis and evaluation of anti-HIV activity of isatin beta- ...
... s can bind to other proteins as well as to small-molecule substrates. When proteins bind specifically to other copies of ... Quaternary structure: the structure formed by several protein molecules (polypeptide chains), usually called protein subunits ... Kauzmann W (1956). "Structural factors in protein denaturation". Journal of Cellular Physiology. Supplement. 47 (Suppl 1): 113- ... The membrane alone has a hydrophobic core through which polar or charged molecules cannot diffuse. Membrane proteins contain ...
Beta oxidation is the metabolic process by which fatty acids are broken down in the mitochondria or in peroxisomes to generate ... Malinauskas T (2008). "Docking of fatty acids into the WIF domain of the human Wnt inhibitory factor-1". Lipids. 43 (3): 227-30 ... The steroids, all derived from the same fused four-ring core structure, have different biological roles as hormones and ... Polyketides are synthesized by polymerization of acetyl and propionyl subunits by classic enzymes as well as iterative and ...
NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6. Pfam PF09782 Assembly factor proteins[31] ... Core Subunitsa 1. NDUFS7 / PSST / NUKM. NDUS7_HUMAN. NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial EC ... Complex I contains a ubiquinone binding pocket at the interface of the 49-kDa and PSST subunits. Close to iron-sulfur cluster ... A possible quinone exchange path leads from cluster N2 to the N-terminal beta-sheet of the 49-kDa subunit.[26] All 45 subunits ...
Leucine zipper coiled-coil helices & DNA-binding helices: transcription factor Max (PDB file 1HLO) ... The Hemoglobin molecule has four heme-binding subunits, each made largely of α-helices. ... in the hydrophobic core, and one containing predominantly polar amino acids oriented toward the solvent-exposed surface of the ... "The discovery of the alpha-helix and beta-sheet, the principal structural features of proteins". Proceedings of the National ...
X-ray crystallographic structure of the 30S ribosomal subunit with bound drug (purple, space-filling model, at center) protein ... Volume 1. Core Clinical Skills. Oxford University Press. 2010. *^ referenced in Treatment of Mitochodrial Disease: Bindu LH, ... In the past, the aminoglycosides have been used in conjunction with beta-lactam antibiotics in streptococcal infections for ... PubMed:19031229] See Also Fischel-Ghodsian N. Genetic factors in aminoglycoside toxicity. Ann NY Acad Sci. 1999; 884:99-109. [ ...
Diagram of the 12 subunit catalytic core of the cubic 24 subunit human E2k. [18] (C) Ribbon diagram of the 8 Monomer E3 subunit ... The first factor was a decrease in transcription (mRNA levels) of the subunits comprising α-KGDHC. As shown in Figure 13, after ... It is an 8 monomer that can be divided into 4 domains; the FAD-binding domain (residues 1-149), the NAD+-binding domain ( ... In addition, thiamine deficiency and oxidative stress can be linked to the formation of the beta-amyloid(Aβ) plaques, and this ...
positive regulation of core promoter binding. • interleukin-12-mediated signaling pathway. • regulation of regulatory T cell ... positive regulation of tumor necrosis factor production. • CD8-positive, alpha-beta T cell differentiation involved in immune ... including subunits of the immunoproteasome (MECL1, LMP2, LMP7), as well as TAP and ERAAP in addition possibly to the direct ... However, in contrast to many other heparan sulfate binding proteins, where binding promotes biological activity, the binding of ...
"New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... Hobbs K, Negri J, Klinnert M, Rosenwasser LJ, Borish L (December 1998). "Interleukin-10 and transforming growth factor-beta ... DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-13. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ...
... is not required for non-primate lentiviral Vif-mediated APOBEC3 degradation.. ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the ... The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the ... produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion ...
Comprehensive view of all products targeting Core-binding factor beta subunit (CBFB) (CBFbeta). Includes lead product status, ... Core-binding factor b-subunit (CBFB; CBFb); runt-related transcription factor 1 (RUNX1) In vitro and mouse studies suggest ... Therapeutics: Core-binding factor β subunit (CBFB; CBF-β); myosin heavy chain 11 smooth muscle (MYH11; SMMHC); runt-related ... core-binding factor b-subunit (CBFB; CBFb) In vitro and mouse studies suggest the small molecule kartogenin could help treat ...
Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - ... core binding factor to the enhancer of T cell receptor genes,beta subunit,altered in acute myeloid leukemia (AML-M4Eo) with ... CBFB (Core-Binding Factor Beta Subunit) is a Protein Coding gene. Diseases associated with CBFB include Acute Myeloid Leukemia ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the ...
core-binding factor subunit beta. Enable Javascript to view the expand/collapse boxes.. Printable PDF Open All Close All ... OMIM: CORE-BINDING FACTOR, BETA SUBUNIT. *. Eghtedar A, Borthakur G, Ravandi F, Jabbour E, Cortes J, Pierce S, Kantarjian H, ... The CBFB gene provides instructions for making a protein called core binding factor beta (CBFβ), which is one piece of a ... Yoshida CA, Furuichi T, Fujita T, Fukuyama R, Kanatani N, Kobayashi S, Satake M, Takada K, Komori T. Core-binding factor beta ...
Compare Anti-nuclear transcription factor Y subunit beta Antibody Products from leading suppliers on Biocompare. View ... anti-Core-Binding Factor, beta Subunit (CBFB) (Internal Region) antibody *. Applications: WB, ELISA ... Your search returned 139 nuclear transcription factor Y subunit beta Antibodies across 25 suppliers. ... Anti-nuclear transcription factor Y subunit beta Antibody Products. Anti-nuclear transcription factor Y subunit beta Antibody ...
... which is one piece of a protein complex known as core binding factor (CBF). CBFβ attaches (binds) to one of three related RUNX ... These protein complexes bind to specific regions of DNA and help turn on (activate) certain genes.The presence of CBFβ helps ... the complex bind to DNA and protects the RUNX protein from being broken down. Learn about this gene and related health ... The CBFB gene provides instructions for making a protein called core binding factor beta (CBFβ), ...
Core-binding factor subunit beta. B, G. 142. Mus musculus. Mutation(s): 0 Gene Names: Cbfb, Pebp2b, Pebpb2. ... Runt-related transcription factor 1. A, F. 204. Mus musculus. Mutation(s): 1 Gene Names: Aml1, Cbfa2, Pebp2ab, Runx1. ... Cooperative assemblies of transcription factors (TFs) on target gene enhancers coordinate cell proliferation, fate ... Cooperative assemblies of transcription factors (TFs) on target gene enhancers coordinate cell proliferation, fate ...
CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN-CBFBETA CORE DOMAIN HETERODIMER AND C/EBPBETA BZIP HOMODIMER BOUND TO A ... CORE-BINDING FACTOR, BETA SUBUNIT. D. 141. Mus musculus. Mutation(s): 0 Gene Names: Cbfb (Pebp2b, Pebpb2). ... The core binding factor (CBF) heterodimeric transcription factors comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta ... The core binding factor (CBF) heterodimeric transcription factors comprised of AML/CBFA/PEBP2alpha/Runx and CBFbeta/PEBP2beta ...
Core-binding factor, beta subunit. XENTR. 298. UniRef50_Q6P814. Nuclear Y/CCAAT-box binding factor A subunit NF-YA. XENLA ... UniProt is an ELIXIR core data resource. Main funding by: National Institutes of Health. The European Molecular Biology ...
... the underlying transcription factors (TFs) governing such expression cha … ... Core Binding Factor Alpha 2 Subunit / genetics * Core Binding Factor Alpha 2 Subunit / metabolism ... Hepatocyte Nuclear Factor 3-beta / genetics * Hepatocyte Nuclear Factor 3-beta / metabolism ... In this study, we propose a method to integrate gene expression with TF binding data and protein-protein interactions to ...
core-binding factor, beta subunit [Source:HGNC Symbol;Acc:1539]. Mouse Orthologue:. Cbfb. Mouse Description:. core binding ... core-binding factor subunit beta [Source:RefSeq peptide;Acc:NP_954679]. Human Orthologue:. CBFB. Human Description:. ...
Core Binding Factor Alpha 1 Subunit / genetics * Core Binding Factor Alpha 1 Subunit / metabolism ... They were noted to enhance the mRNA expression of BMP-2, BMP-4, Runx2, beta-catenin and cyclinD1, all of which are BMP or Wnt- ... The osteogenic effect was inhibited by the introduction of noggin and DKK-1, which is classical inhibitor of BMP and Wnt/beta- ... Flavonoids of Herba Epimedii regulate osteogenesis of human mesenchymal stem cells through BMP and Wnt/beta-catenin signaling ...
CBFB - Core-Binding Factor, beta Subunit: CBFB Antikörper CBFB Proteine CREBBP - CBP: CREBBP Antikörper CREBBP ELISA Kits ... NFYB (Nuclear Transcription Factor Y, beta): NFYB Antikörper NFYB Proteine NFYC - Nuclear Transcription Factor Y, gamma: NFYC ... SP1 (Sp1 Transcription Factor): SP1 Antikörper SP1 ELISA Kits SP1 Proteine SREBF1 (Sterol Regulatory Element Binding ... MED12 (Mediator Complex Subunit 12): MED12 Antikörper MED12 ELISA Kits MED13 (Mediator Complex Subunit 13): MED13 Antikörper ...
CBFB: core-binding factor subunit beta. *CBS: cystathionine-beta-synthase. *CCBE1: collagen and calcium binding EGF domains 1 ...
Mutagenesis of the Runt domain defines two energetic hot spots for heterodimerization with the core binding factor beta subunit ... RUNX1 encodes the α subunit that dimerizes with a β subunit to yield a functional core binding factor (CBF) heterodimeric ... Sequence specificity of the core-binding factor. J Virol. 1993;67(4):2408-2411.. View this article via: PubMed Google Scholar ... RUNX1/core binding factor A2 regulates platelet 12-lipoxygenase gene (ALOX12): studies in human RUNX1 haplodeficiency. Blood. ...
CBFB; core-binding factor, beta subunit; core-binding factor subunit beta; PEBP2B; CBF-beta; PEA2-beta; PEBP2-beta; SL3-3 ... enhancer factor 1 beta subunit; SL3-3 enhancer factor 1 subunit beta; SL3/AKV core-binding factor beta subunit; polyomavirus ... Antigen standard for core-binding factor, beta subunit (CBFB), transcript variant 2 is a lysate prepared from HEK293T cells ... enhancer-binding protein 2 beta subunit; polyomavirus enhancer binding protein 2, beta subunit;. ...
Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by the alpha subunit as the ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family ...
PE Linked Polyclonal Antibody to Core Binding Factor Beta Subunit CBFb. Supplier:MyBioSource ... small amounts may get caught on the vials walls and/or lid due to shipping and handling factors. We recommend you to briefly ...
Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (CBFA2T3), transcript variant 2 ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4 ... Adenovirus with ORF of interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40 ...
The five genes identified by all the protocols were DIRAS3, CXCL6, SAMD5, CBFB (core-binding factor, beta subunit), and MEIS2. ... core-binding factor, beta subunit), and MEIS2 (meis homeobox 2). Real time PCR experiments verified results on a subset of ... latent transforming growth factor beta binding protein 2), a primary glaucoma causing gene, was not probed on the arrays [42]. ... Loss of function mutations in the gene encoding latent transforming growth factor beta binding protein 2, LTBP2, cause primary ...
The researchers genetically inhibited both RUNX1 and an associated protein called core-binding factor subunit beta (Cbfb). By ... Scientists targeted a protein that is a transcription factor known as RUNX1, which also plays an important role in helping ...
Mutagenesis of the Runt domain defines two energetic hot spots for heterodimerization with the core binding factor beta subunit ... CIR, a corepressor linking the DNA binding factor CBF1 to the histone deacetylase complex. Proc. Natl. Acad. Sci. USA 96:23-28. ... Molecular cloning and characterization of PEBP2 beta, the heterodimeric partner of a novel Drosophila runt-related DNA binding ... is a transcription factor that forms a heterodimer with a non-DNA-binding protein, CBFβ (31, 41). The t(8;21) translocation ...
A new study reveals that the protein Cbf-beta switches progenitor cells from creating bone-producing cells to creating fat ... the team found that a protein called core-binding factor subunit beta (Cbf-beta) was vital for switching destinations between ... Further investigation showed that when Cbf-beta activates a signal inside a type of cell known as Wnt10b/beta-catenin, it ... Could new bone-forming growth factor reverse osteoporosis? Scientists have discovered a new bone-forming growth factor that ...
Miller J, Horner A, Stacy T, Lowrey C, Lian JB, Stein G, Nuckolls GH, Speck NA (2002) The core-binding factor beta subunit is ... Core-binding factor beta interacts with Runx2 and is required for skeletal development. Nat Genet 32:633-638PubMedCrossRef ... Munoz-Sanjuan I, Simandl BK, Fallon JF, Nathans J (1999) Expression of chicken fibroblast growth factor homologous factor (FHF ... Systemic administration of insulin-like growth factor (IGF)-binding protein-4 (IGFBP-4) increases bone formation parameters in ...
core-binding factor subunit beta Gene Symbol:. cbfb Sequence Ontology ID :. SO:0000704. ...
Core Binding Factor alpha Subunits. *Core Binding Factor beta Subunit. *DNA/metabolism ... Mammalian CBFB encodes a transcription factor (CBFbeta) that in combination with CBFalpha2 binds to specific DNA sequences and ... In biochemical analyses, cbfbeta binds to human CBFalpha2 and enhances its DNA binding. During zebrafish development, cbfb is ... expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors ...
... they investigated the key role played by Cbf-beta, or core-binding factor subunit beta. ... In nonmutant mice, they found that the protein complex composed of Cbf-beta and the Runx2 transcription factor binds to the ... The Cbf-beta/Runx2 complex also inhibited expression of the enhancer protein C/EBP-alpha that promotes differentiation of ... The team led by Li and Chen generated three mouse models by deleting Cbf-beta at various stages of the osteoblast lineage. All ...
metal ion binding Source: UniProtKB-KW. *RNA polymerase II core binding Source: SGD ,p>Inferred from Physical Interaction,/p> , ... help/subunit_structure target=_top>More...,/a>,/p>Subunit structurei. TFIIE is a tetramer of two alpha (TFA1) and two beta ( ... TFA2) subunits.By similarity. GO - Molecular functioni. *RNA polymerase II core binding Source: SGDInferred from physical ... sp,P36100,T2EA_YEAST Transcription initiation factor IIE subunit alpha OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c ...
Inactivation of the gene encoding RUNX cofactor core-binding factor-beta (CBFbeta) in mice and small interfering RNA (siRNA)- ... Core Binding Factor Alpha 2 Subunit/genetics/immunology*. *Core Binding Factor Alpha 3 Subunit/genetics/immunology* ... Inactivation of the gene encoding RUNX cofactor core-binding factor-beta (CBFbeta) in mice and small interfering RNA (siRNA)- ... we show that the transforming growth factor-beta (TGF-beta) induces the expression of the Runt-related transcription factors ...
CBFB, encoding core-binding factor beta subunit, was mutated in four ER+ tumors. CBFB is a heterodimeric partner with the RUNX ... These fusions retained the kinase domain of AKT3 while disrupting the pleckstrin homology and PTEN-binding domains of AKT3 and ... This suggests that the mechanisms and environmental factors contributing to the development and progression of breast tumors ... the relatively small region of tumor in a core biopsy may still miss a significant percentage of the clonal lesions present in ...
  • CBFB enhances DNA binding by RUNX1. (nih.gov)
  • uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a fluorescence resonance energy transfer (FRET) assay. (nih.gov)
  • Confirmation of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a time resolved fluorescence resonance energy transfer (TR-FRET) assay. (nih.gov)
  • CBFB (Core-Binding Factor Beta Subunit) is a Protein Coding gene. (genecards.org)
  • The CBFB gene provides instructions for making a protein called core binding factor beta (CBFβ), which is one piece of a protein complex known as core binding factor (CBF). (nih.gov)
  • Antigen standard for core-binding factor, beta subunit (CBFB), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. (creativebiomart.net)
  • Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. (wikipedia.org)
  • Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. (trademetro.net)
  • The five genes were DIRAS3 (DIRAS family, GTP-binding RAS-like 3), CXCL6 (chemokine (C-X-C motif) ligand 6), SAMD5 (sterile alpha motif domain containing 5), CBFB (core-binding factor, beta subunit), and MEIS2 (meis homeobox 2). (molvis.org)
  • The researchers genetically inhibited both RUNX1 and an associated protein called core-binding factor subunit beta (Cbfb). (oncologynurseadvisor.com)
  • Mammalian CBFB encodes a transcription factor (CBFbeta) that in combination with CBFalpha2 binds to specific DNA sequences and regulates expression of a number of hematopoietic genes. (zfin.org)
  • We have also analyzed the expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors to cbfb in zebrafish hematopoiesis. (zfin.org)
  • Polyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH-conjugated synthetic peptides containing sequences from the central region of the protein. (diagenode.com)
  • CBFb (UniProtKB/Swiss-Prot entry Q13951) represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. (diagenode.com)
  • A common mutation in AML is the inversion of chromosome 16 [inv (16)], which generates a fusion between the genes for core binding factor beta (CBFB) and smooth muscle myosin heavy chain gene (MYH11), forming the oncogene CBFB-MYH11. (elsevier.com)
  • The expressed protein, CBFb-SMMHC, forms a heterodimer with the key hematopoietic transcription factor RUNX1. (elsevier.com)
  • Beyond confirming recurrent somatic mutations in PIK3CA, TP53, AKT1, GATA3 and MAP3K1, we discovered recurrent mutations in the CBFB transcription factor gene and deletions of its partner RUNX1. (nih.gov)
  • A. CBFB coding region diagram: RUNX binding domain in green. (nih.gov)
  • Cbfb, which is a co-transcription factor that forms a heterodimer with Runx2, enhances DNA binding of Runx2 and stabilizes Runx2 protein by inhibiting its ubiquitination. (inforang.com)
  • 1982 ). Further study revealed that PEBP2/CBFB was composed of dimers of a DNA-binding subunit (CBF-α) and a non-DNA-binding subunit (CBF-β). (springer.com)
  • Mechanistically, the Runx3 - CBFbeta (show CBFB ELISA Kits ) transcription factor complex deployed H3K27me3 to Bcl6 (show BCL6 ELISA Kits ) and Tcf7 (show TCF7 ELISA Kits ) genes to suppress the TFH program. (antibodies-online.com)
  • runt-related transcription factor 1 (RUNX1) In vitro and mouse studies suggest inhibiting. (biocentury.com)
  • CBFβ attaches (binds) to one of three related RUNX proteins (RUNX1, RUNX2, or RUNX3) to form different versions of CBF. (nih.gov)
  • Once bound to DNA, the RUNX1 protein controls the activity of genes involved in the development of blood cells (hematopoiesis). (nih.gov)
  • The protein produced from the fusion gene, called CBFβ-MYH11, can still bind to RUNX1 to form CBF. (nih.gov)
  • The presence of CBFβ-MYH11 may block binding of CBF to DNA, preventing RUNX1 from controlling gene activity. (nih.gov)
  • Crystallographic analysis of a complex comprising Ets1, Runx1, and CBFβ at the TCRα enhancer revealed that Ets1 acquires robust binding stability in the Runx1 and DNA-complexed state, via allosteric mechanisms. (rcsb.org)
  • The best understood of these genes encode transcription factors, such as GATA-2, RUNX1, ETV6, and C/EBPα, which establish and maintain genetic networks governing the genesis and function of blood stem and progenitor cells. (jci.org)
  • Scientists targeted a protein that is a transcription factor known as RUNX1, which also plays an important role in helping regulate the normal development of blood cells. (oncologynurseadvisor.com)
  • Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. (nih.gov)
  • In this study, we show that the transforming growth factor-beta (TGF-beta) induces the expression of the Runt-related transcription factors RUNX1 and RUNX3 in CD4(+) T cells. (nih.gov)
  • This induction seems to be a prerequisite for the binding of RUNX1 and RUNX3 to three putative RUNX binding sites in the FOXP3 promoter. (nih.gov)
  • Inactivation of the gene encoding RUNX cofactor core-binding factor-beta (CBFbeta) in mice and small interfering RNA (siRNA)-mediated suppression of RUNX1 and RUNX3 in human T cells resulted in reduced expression of Foxp3. (nih.gov)
  • A common chromosomal translocation in acute myeloid leukemia (AML) involves the AML1 (acute myeloid leukemia 1, also called RUNX1, core binding factor protein (CBF alpha), and PEBP2 alpha B) gene on chromosome 21 and the ETO (eight-twenty one, also called MTG8) gene on chromosome 8. (curehunter.com)
  • Cdk6 inhibits Runx1 activity by binding to the runt domain of Runx1, interfering with Runx1 DNA binding and Runx1‐C/EBPα interaction. (embopress.org)
  • Runx2 is a transcription factor that belongs to the Runx family composed of Runx1, Runx2, and Runx3. (inforang.com)
  • Zebrafish embryos lacking Rad21 (show RAD21 ELISA Kits ), or cohesin subunit Smc3 (show SMC3 ELISA Kits ), fail to express runx3 and lose hematopoietic runx1 (show RUNX1 ELISA Kits ) expression in early embryonic development. (antibodies-online.com)
  • AML1-ETO interferes with the function of the AML1 (RUNX1, CBFA2) transcription factor in a dominant-negative fashion and represses transcription by binding its consensus DNA-binding site and via protein-protein interactions with other transcription factors. (bloodjournal.org)
  • 1 2 The CBFα (AML1/RUNX1) subunit binds DNA directly, whereas the CBFβ subunit enhances binding of CBFα to DNA but does not bind DNA itself. (bloodjournal.org)
  • While three mammalian genes encode α-subunits: RUNX1 (PEBP2αB), RUNX2 (PEBP2αA) and RUNX3 (PEBP2αC), only one gene has been identified for the β-subunit (PEBP2β/CBFβ). (biomedcentral.com)
  • These proteins are collectively referred to as type II DNA-binding proteins (DBPII), forming a group of basic, dimeric proteins found in all bacteria that are able to bind DNA to induce and stabilise DNA bending. (ebi.ac.uk)
  • Restriction factors, such as the retroviral complementary DNA deaminase APOBEC3G, are cellular proteins that dominantly block virus replication. (nih.gov)
  • CBF-β, which normally functions in concert with RUNX DNA binding proteins, allows the reconstitution of a recombinant six-protein assembly that elicits specific polyubiquitination activity with APOBEC3G, but not the related deaminase APOBEC3A. (nih.gov)
  • The TFIIE beta central core DNA-binding domain consists of three helices with a beta hairpin at the C terminus, resembling the winged helix proteins. (ebi.ac.uk)
  • This entry represents the conserved amino terminal region of eukaryotic TFIIE-alpha and proteins from archaebacteria (TFE) that are also presumed to be TFIIE-alpha subunits [ PMID: 9389475 ]. (ebi.ac.uk)
  • The cDNA clones, which we call CBF beta p22.0, CBF beta p21.5, and CBF beta p17.6, encode three highly related but distinct proteins with deduced molecular sizes of 22.0, 21.5, and 17.6 kDa that appear to be translated from multiply spliced mRNAs transcribed from the same gene. (asm.org)
  • In studies on murine polyoma and leukemia viruses, mammalian core-binding factors (CBFs) were identified as proteins regulating viral replication (Boral et al. (springer.com)
  • Proteins belonging to the MADS family function as dimers, the primary DNA-binding element of which is an anti-parallel coiled coil of two amphipathic alpha-helices, one from each subunit. (embl.de)
  • The analysis leads to a better insight of proteins that bind to DNA, regulate DNA, and function in chromatin remodeling. (biomedcentral.com)
  • Genes in eukaryotic cells are regulated in "active regions," in which chromatin structure is "open" and accessible to DNA-binding proteins and "silent regions" where "packed" chromatin renders the DNA inaccessible. (biomedcentral.com)
  • An important motif presented in the promoter is an A/T-rich region that binds proteins with an A/T-hook motif. (biomedcentral.com)
  • At the core, the bHLH-PAS transcriptional activators CLOCK and BMAL1 activate the Period ( Per1, Per2 ) and Cryptochrome ( Cry1, Cry2 ) genes, whose transcripts and proteins slowly accumulate during the daytime. (hhmi.org)
  • Under oxidative stress, NRF2 heterodimerizes with small Maf proteins and binds cis-acting enhancer sequences found near oxidative stress response genes. (hindawi.com)
  • The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g. (nih.gov)
  • The gene is highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit gene. (wikipedia.org)
  • CBFa(AML/PEBP2)-related elements in the TGF-beta type I receptor promo" by Changhua Ji, Sandra Casinghino et al. (umassmed.edu)
  • Investigating the regulatory mechanism of viral replication led to the identification of the polyomavirus enhancer-binding protein 2 (PEBP2), also known as core-binding factor-β (CBF-β) (Katinka et al. (springer.com)
  • 1998). "The chimeric protein, PEBP2 beta/CBF beta-SMMHC, disorganizes cytoplasmic stress fibers and inhibits transcriptional activation. (thefullwiki.org)
  • Edelta function depends critically on transcription factors core binding factor (CBF)/polyoma enhancer-binding protein 2 (PEBP2) and c-Myb as measured by transcriptional activation of transiently transfected substrates in Jurkat cells, and by activation of V(D)J recombination within chromatin-integrated substrates in transgenic mice. (duke.edu)
  • Our data indicate that CBF/PEBP2 plays primarily a structural role as it induces a conformational change in the enhanceosome that is associated with augmented binding of c-Myb. (duke.edu)
  • In contrast, c-Myb has no apparent affect on CBF/PEBP2 binding, but is critical for transcriptional activation. (duke.edu)
  • They were noted to enhance the mRNA expression of BMP-2, BMP-4, Runx2, beta-catenin and cyclinD1, all of which are BMP or Wnt-signaling pathway related regulators. (nih.gov)
  • In nonmutant mice, they found that the protein complex composed of Cbf-beta and the Runx2 transcription factor binds to the Wnt10b promoter to drive Wnt10b expression. (news-medical.net)
  • The Cbf-beta/Runx2 complex also inhibited expression of the enhancer protein C/EBP-alpha that promotes differentiation of adipocytes. (news-medical.net)
  • Runx2 is an essential transcription factor for skeletal development. (inforang.com)
  • Core-binding factor beta interacts with Runx2 and is required for skeletal development. (labome.org)
  • MBS934869 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. (mybiosource.com)
  • Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. (mybiosource.com)
  • [1] It is a bacterial transcription initiation factor that enables specific binding of RNA polymerase to gene promoters . (wikipedia.org)
  • The presence of CBFβ helps the complex bind to DNA and protects the RUNX protein from being broken down. (nih.gov)
  • Here, we present the crystal structures of the AML1/Runx-1/CBFalpha(Runt domain)-CBFbeta(core domain)-C/EBPbeta(bZip)-DNA, AML1/Runx-1/CBFalpha(Runt domain)-C/EBPbeta(bZip)-DNA, and AML1/Runx-1/CBFalpha(Runt domain)-DNA complexes. (rcsb.org)
  • Circulating human CD4(+) CD25(high) CD127(-) T reg cells significantly expressed higher levels of RUNX3, FOXP3, and TGF-beta mRNA compared with CD4(+)CD25(-) cells.Furthermore, FOXP3 and RUNX3 were colocalized in human tonsil T reg cells.These data demonstrate Runx transcription factors as a molecular link in TGF-beta-induced Foxp3 expression in iT reg cell differentiation and function. (nih.gov)
  • These data demonstrate Runx transcription factors as a molecular link in TGF-beta-induced Foxp3 expression in iT reg cell differentiation and function. (nih.gov)
  • They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. (dartmouth.edu)
  • Since Runx transcription factors play central roles in hematopoietic, neuronal and osteogenic lineages, this novel, noncanonical Cdk6 function may control terminal differentiation in multiple tissues and cell types. (embopress.org)
  • The Runx genes encode α-subunits of heterodimeric transcription factors which regulate cell proliferation and differentiation in various tissues during development and which can become leukemogenic upon aberrant expression. (biomedcentral.com)
  • Here we have identified novel protein-protein interactions between the Pim-1 kinase and the RUNX family transcription factors. (biomedcentral.com)
  • Altogether, our results suggest that mammalian RUNX family transcription factors are novel binding partners and substrates for the Pim-1 kinase, which may be able to regulate their activities during normal hematopoiesis as well as in leukemogenesis. (biomedcentral.com)
  • There is less sequence similarity between RUNX family members outside the Runt domain, except for the highly conserved five amino acid C-terminus (VWRPY) known to bind transcriptional repressors, but the C-terminal regions are rich in proline, threonine and serine (PTS) and contain domains involved in transcriptional activation or inhibition [ 19 ]. (biomedcentral.com)
  • Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. (harvard.edu)
  • Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. (dartmouth.edu)
  • The CLOCK protein acts as a heterodimeric transcription factor with a partner known as BMAL1 (ARNTL). (hhmi.org)
  • Cooperative assemblies of transcription factors (TFs) on target gene enhancers coordinate cell proliferation, fate specification, and differentiation through precise and complicated transcriptional mechanisms. (rcsb.org)
  • When Notch-IC enters the nucleus, its binding to RBP-Jκ may trigger an allosteric change that facilitates the displacement of the transcriptional corepressor complex. (asm.org)
  • SRF function is essential for transcriptional regulation of numerous growth-factor-inducible genes, such as c-fos oncogene and muscle-specific actin genes. (embl.de)
  • The study summarizes transcriptional regulation factors interacting and cooperating at promoter regions that regulate gene expression. (biomedcentral.com)
  • Transcriptional regulation factors interacting and cooperating at promoter regions determine the fate of cells and their function. (biomedcentral.com)
  • The waxing and waning of this transcriptional feedback loop takes approximately 24 hours and represents the core mechanism of the circadian clock in mammals. (hhmi.org)
  • Nuclear factor- (erythroid-derived 2) like 2 ( NFE2L2, NRF2 ) is a key transcriptional activator of the antioxidant response pathway and is closely related to erythroid transcription factor NFE2 . (hindawi.com)
  • it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. (nih.gov)
  • In biochemical analyses, cbfbeta binds to human CBFalpha2 and enhances its DNA binding. (zfin.org)
  • The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. (diagenode.com)
  • The hydrogen bonding network formed among CBFalpha(Runt domain) and CBFbeta, and CBFalpha(Runt domain) and DNA revealed the allosteric regulation mechanism of CBFalpha(Runt domain)-DNA binding by CBFbeta. (rcsb.org)
  • We have previously shown that the transcription factor PLAGL2 promotes proliferation and cooperates with the leukemia fusion protein Cbfbeta-SMMHC in AML development. (umassmed.edu)
  • In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. (nih.gov)
  • The pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript that encodes a protein consisting of the first 165 residues from the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain. (genetex.com)
  • A heterodimer of RUNX3 and a beta subunit form a complex found in a number of enhancers and promoters, and together either activate or suppress transcription. (thefreedictionary.com)
  • The protein is a heterodimer of alpha- and beta-subunits. (wikipedia.org)
  • RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa , a beta (β) subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. (wikipedia.org)
  • A gene on chromosome 1p36 that encodes a member of the runt domain-containing family of transcription factors. (thefreedictionary.com)
  • The region of shared similarity, known as the Runt domain, is responsible for DNA-binding and protein-protein interaction. (wikipedia.org)
  • In addition to the highly conserved Runt domain, the AML-1 gene product carries a putative ATP-binding site (GRSGRGKS), and has a C-terminal region rich in proline and serine residues. (wikipedia.org)
  • The β-subunit can enhance DNA-binding by the Runt domain but does not contact DNA itself [ 15 , 18 ]. (biomedcentral.com)
  • In this study, we propose a method to integrate gene expression with TF binding data and protein-protein interactions to construct an integrated regulatory network (IRN) for endometriosis. (nih.gov)
  • Antp is a sequence-specific transcription factor which is part of a developmental regulatory system that regulates segmental identity in the mesothorax. (antibody-antibodies.com)
  • A major determinant of the thymic disease specificity of Moloney virus genetically maps to the conserved viral core motif in the Moloney virus enhancer. (asm.org)
  • Point mutations introduced into the core site significantly shifted the disease specificity of the Moloney virus from thymic leukemia to erythroid leukemia (N.A. Speck, B. Renjifo, E. Golemis, T.N. Fredrickson, J.W. Hartley, and N. Hopkins, Genes Dev. (asm.org)
  • A sigma factor is the promoter specificity subunit of eubacterial-type multisubunit RNA polymerases, those whose core subunit composition is often described as alpha(2)-beta-beta-prime. (cathdb.info)
  • One of the genes that encodes a CBF alpha subunit is AML1, also called Cbf alpha 2. (curehunter.com)
  • 12-14 However, the use of cell lines may not accurately model the functional effects of AML1-ETO expression, owing to the different complement of transcription factors found in transformed cells versus hematopoietic progenitor cells (HPCs). (bloodjournal.org)
  • AML1-ETO "knock-in" mice do not develop leukemia, and factor-dependent cell lines derived from AML1-ETO-expressing murine progenitors are not leukemogenic when transplanted into irradiated syngeneic or severe combined immunodeficient (SCID) mice. (bloodjournal.org)
  • A sigma (σ) factor binds to the core, forming the holoenzyme. (wikipedia.org)
  • Every molecule of RNA polymerase holoenzyme contains exactly one sigma factor subunit, which in the model bacterium Escherichia coli is one of those listed below. (wikipedia.org)
  • RNA polymerase holoenzyme complex consisting of core RNA polymerase and a sigma factor executes transcription of a DNA template strand. (wikipedia.org)
  • Although sigma does not bind DNA on its own, when combined with the core to form the holoenzyme, this binds specifically to promoter sequences, with the sigma factor making sequence specific contacts with the promoter elements. (cathdb.info)
  • Human serum response factor (SRF) is a ubiquitous nuclear protein important for cell proliferation and differentiation. (embl.de)
  • Your search returned 139 nuclear transcription factor Y subunit beta Antibodies across 25 suppliers. (biocompare.com)
  • By gel mobility shift and immunological analyses, a principal osteoblast-derived nuclear factor that binds to these sites is CBFa1 (AML-3/PEBP2alphaA). (umassmed.edu)
  • Nuclear receptors (NRs) bind to small lipophilic molecules, such as steroids [ 1 ] thyroid hormones and active forms of retinoids [ 2 ]. (hindawi.com)
  • NRF2 (encoded by nuclear factor-erythroid p45-related factor 2, NFE2L2 ) is the master regulator of antioxidant and phase II detoxification genes that collectively resist cellular damage due to electrophilic and oxidative stress [ 1 , 2 ]. (hindawi.com)
  • Core binding factor acute myeloid leukaemia is a cancer related to genetic changes in the CBF gene. (wikipedia.org)
  • Comparison of therapy-related and de novo core binding factor acute myeloid leukemia: A bone marrow pathology group study. (harvard.edu)
  • Core-binding factor acute myeloid leukemia with t(8;21): Risk factors and a novel scoring system (I-CBFit). (harvard.edu)
  • Adding dasatinib to intensive treatment in core-binding factor acute myeloid leukemia-results of the AMLSG 11-08 trial. (harvard.edu)
  • Dasatinib in high-risk core binding factor acute myeloid leukemia in first complete remission: a French Acute Myeloid Leukemia Intergroup trial. (harvard.edu)
  • Population-based disparities in survival among patients with core-binding factor acute myeloid leukemia: a SEER database analysis. (harvard.edu)
  • Prospective evaluation of gene mutations and minimal residual disease in patients with core binding factor acute myeloid leukemia. (harvard.edu)
  • The protein (known as acute myeloid leukemia 1 protein, oncogene AML-1, core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5'-pygpyggt-3', of a number of enhancers and promoters. (wikipedia.org)
  • The recurrent chromosomal translocations found in acute myelogenous leukemia (AML) often involve transcription factors. (bloodjournal.org)
  • Because the genetic changes affect CBF, the condition is classified as core binding factor AML (CBF-AML). (nih.gov)
  • Progenitor cells are immature cells that stand in reserve until they receive genetic instructions from transcription factors that tell them which type of cell to become. (medicalnewstoday.com)
  • Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression. (harvard.edu)
  • Definitive evidence that alterations in chromosomal subunits play a role in cancer was obtained from studies of genes that control cancer growth, and discoveries of the transforming capacity of retroviruses in the 1970s established that virtually all cancers arise from the disruption of genetic material. (aaccjnls.org)
  • GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and transcription coactivator activity . (genecards.org)
  • Integration host factor (IHF) ( IPR005684 , IPR005685 ) is a small heterodimeric protein that binds the minor groove of DNA in a sequence-specific manner and induces a large bend. (ebi.ac.uk)
  • Distinct roles for c-Myb and core binding factor/polyoma enhancer-binding protein 2 in the assembly and function of a multiprotein complex on the TCR delta enhancer in vivo. (duke.edu)
  • Circulating human CD4(+) CD25(high) CD127(-) T reg cells significantly expressed higher levels of RUNX3, FOXP3, and TGF-beta mRNA compared with CD4(+)CD25(-) cells. (nih.gov)
  • Initiation of eukaryotic mRNA transcription requires melting of promoter DNA with the help of the general transcription factors TFIIE and TFIIH. (ebi.ac.uk)
  • Micro-RNAs (miRNAs) are small molecules that modulate the translation of target mRNA through binding to these molecules. (ac.ir)
  • Subsequently, Mastermind binds to Notch-IC/RBP-Jκ and the resulting ternary protein complex recruits coactivators, such as histone acetyltransferase p300 or PCAF ( 22 , 33 , 40 , 44 ), chromatin remodeling factors, and the mediator complex, to activate transcription ( 12 ). (asm.org)
  • The AIDS virus, human immunodeficiency virus type 1 (HIV-1), produces the accessory factor Vif, which counteracts the host's antiviral defence by hijacking a ubiquitin ligase complex, containing CUL5, ELOC, ELOB and a RING-box protein, and targeting APOBEC3G for degradation. (nih.gov)
  • A transcription factor and its associated transcription mediator complex must be attached to a DNA binding site called a promoter region before RNAP can initiate the DNA unwinding at that position. (wikipedia.org)
  • Depending on the organism, a RNA polymerase can be a protein complex (multi-subunit RNAP) or only consist of one subunit (single-subunit SNAP, ssSNAP), each representing an independent lineage. (wikipedia.org)
  • [4] [5] The core RNA polymerase complex forms a "crab claw" or "clamp-jaw" structure with an internal channel running along the full length. (wikipedia.org)
  • Once initiation of RNA transcription is complete, the sigma factor can leave the complex. (wikipedia.org)
  • However, CBF beta p22.0 and CBF beta p21.5 form a complex with DNA-binding CBF alpha subunits and as a result decrease the rate of dissociation of the CBF protein-DNA complex. (asm.org)
  • CBF is a heterodimeric complex that binds to core enhancer elements in viral and cellular genes. (bloodjournal.org)
  • The X-ray crystal structure of the serum response factor core in a specific-recognition DNA complex shows that the functions of DNA binding, dimerization and accessory-factor interaction are compactly integrated into a novel protein unit. (embl.de)
  • Any process involved in the assembly of the RNA polymerase preinitiation complex (PIC) at the core promoter region of a DNA template, resulting in the subsequent synthesis of RNA from that promoter. (cathdb.info)
  • Moreover, these results provide new insight into the heterogeneous ensemble of complexes formed by DnaK chaperones and further emphasize the unique role of NMR spectroscopy in obtaining information about individual events in a complex binding scheme by exploiting a large number of probes that report uniquely on distinct binding processes. (weizmann.ac.il)
  • These chromosomal abnormalities tend to disrupt genes that encode for transcription factors needed for myeloid stem cells to differentiate into specific blood components. (the-medical-dictionary.com)
  • All subunits showed covalent modifications that fall into three classes including retention of initiating formyl-methionine, removal of methionine at the N-terminus with or without acetylation, and removal of a longer N-terminal peptide. (pubmedcentralcanada.ca)
  • After specific ligand binding, the intracellular part of Notch is cleaved off and translocates to the nucleus, where it targets the DNA binding protein RBP-Jκ. (asm.org)
  • At the molecular level, the triggering of the Notch receptor by ligand binding leads to proteolytic processing within the transmembrane domain, which results in the release of the Notch intracellular domain Notch-IC. (asm.org)
  • Molecular recognition is integral to biological function and frequently involves preferred binding of a molecule to one of several exchanging ligand conformations in solution. (weizmann.ac.il)
  • In such a process the bound structure can be selected from the ensemble of interconverting ligands a priori (conformational selection, CS) or may form once the ligand is bound (induced fit, IF). (weizmann.ac.il)
  • F-type ATPases consist of two structural domains, F(1) containing the extramembraneous catalytic core and F(0) containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. (string-db.org)
  • Although detailed pictures of Hsp70 bound with peptides have emerged, correspondingly detailed structural information on complexes with folding-competent substrates remains lacking. (weizmann.ac.il)
  • A sigma factor ( σ factor ) is a protein needed only for initiation of transcription. (wikipedia.org)
  • [2] The specific sigma factor used to initiate transcription of a given gene will vary, depending on the gene and on the environmental signals needed to initiate transcription of that gene. (wikipedia.org)
  • Selection of promoters by RNA polymerase is dependent on the sigma factor that associates with it. (wikipedia.org)
  • For example, σ 70 is the sigma factor with a molecular weight of 70 kDa . (wikipedia.org)
  • σ 70 (RpoD) - σ A - the "housekeeping" sigma factor or also called as primary sigma factor , transcribes most genes in growing cells. (wikipedia.org)
  • Every cell has a "housekeeping" sigma factor that keeps essential genes and pathways operating. (wikipedia.org)
  • [1] In the case of E. coli and other gram-negative rod-shaped bacteria, the "housekeeping" sigma factor is σ 70 . (wikipedia.org)
  • σ 32 (RpoH) - the heat shock sigma factor, it is turned on when the bacteria are exposed to heat. (wikipedia.org)
  • There are also anti-sigma factors that inhibit the function of sigma factors and anti-anti-sigma factors that restore sigma factor function. (wikipedia.org)
  • [6] Eukaryotic and archaeal RNA polymerases have a similar core structure and work in a similar manner, although they have many extra subunits. (wikipedia.org)
  • CCAAT/enhancer binding protein-mediated regulation of TGF? (labome.org)
  • The C_EBP family of transcription factors regulates viral and cellular CCAAT_enhancer element-mediated transcription. (antibody-antibodies.com)
  • Several sequences in the rat TRI promoter comprise cis-acting elements for CBFa (AML/PEBP2alpha) transcription factors. (umassmed.edu)
  • In the journal PNAS , scientists from both the University of Alabama at Birmingham and Zhejiang University in China explain how a protein called Cbf-beta is important for controlling the rate at which new bone cells replace old ones. (medicalnewstoday.com)
  • When they investigated the transcription factors controlling the direction of maturity, the team found that a protein called core-binding factor subunit beta (Cbf-beta) was vital for switching destinations between bone-producing cell and fat cell. (medicalnewstoday.com)
  • The UAB researchers found that a protein called Cbf-beta plays a critical role in maintaining the bone-producing cells. (news-medical.net)
  • CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters. (nih.gov)
  • These specialized sigma factors bind the promoters of genes appropriate to the environmental conditions, increasing the transcription of those genes. (wikipedia.org)
  • Activated NRF2 bound the antioxidant response element (ARE) in promoters of several known and novel target genes involved in iron homeostasis and heme metabolism, including known targets FTL and FTH1 , as well as novel binding in the globin locus control region. (hindawi.com)
  • Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. (nih.gov)
  • The proposed mechanism of ATRA action involves binding of ATRA to the fusion protein promyelocytic leukemia (PML)/retinoic acid receptor α (RARα) that is encoded by a translocation involving PML gene on chromosome 15 and RARA gene on chromosome 17, and which is assumed to act as a co-repressor. (biomedcentral.com)
  • To test this hypothesis, we established a novel erythropoietin (Epo) independent UT-7/EN cell line from UT-7/Epo, an Epo-dependent megakaryocytic leukemia cell line. (tamuc.edu)
  • Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. (asm.org)
  • CBF binds to core sites in murine leukemia virus and T-cell receptor enhancers. (asm.org)
  • Here we illustrate mechanistic advances in our understanding of familial MDS/AML syndromes caused by germline mutations of hematopoietic transcription factors. (jci.org)
  • ERG and FLI1 are closely related members of the ETS family of transcription factors and have been identified as essential factors for the function and maintenance of normal hematopoietic stem cells. (diagenode.com)
  • Assistance at UAB also was provided by the Center for Metabolic Bone Disease, the Small Animal Phenotyping Core, the Metabolism Core and the Neuroscience Molecular Detection Core Laboratory. (eurekalert.org)
  • Sigma factors are distinguished by their characteristic molecular weights. (wikipedia.org)
  • To understand the molecular mechanisms for synergy between these transcription factors in the context of chromatin, we used in vivo footprinting to study the requirements for protein binding to Edelta within wild-type and mutant versions of a human TCR delta minilocus in stably transfected Jurkat cells. (duke.edu)
  • In an effort to characterize the interactions further we report here the results of an NMR-based titration study of hTRF1 and DnaK, where both molecular components are monitored simultaneously, leading to a binding model. (weizmann.ac.il)