A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.
Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.
A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
Myosin type II isoforms found in smooth muscle.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.
Single chains of amino acids that are the units of multimeric PROTEINS. Multimeric proteins can be composed of identical or non-identical subunits. One or more monomeric subunits may compose a protomer which itself is a subunit structure of a larger assembly.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
Autosomal dominant syndrome in which there is delayed closing of the CRANIAL FONTANELLES; complete or partial absence of the collarbones (CLAVICLES); wide PUBIC SYMPHYSIS; short middle phalanges of the fifth fingers; and dental and vertebral anomalies.
A type of fibrous joint between bones of the head.
A bone on the ventral side of the shoulder girdle, which in humans is commonly called the collar bone.
An extra tooth, erupted or unerupted, resembling or unlike the other teeth in the group to which it belongs. Its presence may cause malposition of adjacent teeth or prevent their eruption.
A normal developing tooth which has not yet perforated the oral mucosa or one that fails to erupt in the normal sequence or time interval expected for the type of tooth in a given gender, age, or population group.
Congenital absence of or defects in structures of the jaw.
One of a pair of excretory organs (mesonephroi) which grows caudally to the first pair (PRONEPHROI) during development. Mesonephroi are the permanent kidneys in adult amphibians and fish. In higher vertebrates, proneprhoi and most of mesonephroi degenerate with the appearance of metanephroi. The remaining ducts become WOLFFIAN DUCTS.
Progenitor cells from which all blood cells derive.
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
The gamete-producing glands, OVARY or TESTIS.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
The processes whereby the internal environment of an organism tends to remain balanced and stable.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.
A large multisubunit complex that plays an important role in the degradation of most of the cytosolic and nuclear proteins in eukaryotic cells. It contains a 700-kDa catalytic sub-complex and two 700-kDa regulatory sub-complexes. The complex digests ubiquitinated proteins and protein activated via ornithine decarboxylase antizyme.
Leukocytes with abundant granules in the cytoplasm. They are divided into three groups according to the staining properties of the granules: neutrophilic, eosinophilic, and basophilic. Mature granulocytes are the NEUTROPHILS; EOSINOPHILS; and BASOPHILS.
An allosteric enzyme that regulates glycolysis by catalyzing the transfer of a phosphate group from ATP to fructose-6-phosphate to yield fructose-1,6-bisphosphate. In the humans, 6-phosphofructose-1-kinase isozyme C is found in platelets, brain, heart, kidney, colon and testis. This isozyme C can exist as the homotetramer of C subunits (P subunits), or heterotetramer of C type and L type subunits.
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.

A mechanism of repression by acute myeloid leukemia-1, the target of multiple chromosomal translocations in acute leukemia. (1/119)

AML1 is one of the most frequently translocated genes in human leukemia. Here we demonstrate that acute myeloid leukemia-1 (AML-1) (Runx-1) represses transcription from a native promoter, p21(Waf1/Cip1). Unexpectedly, this repression did not require interactions with the Groucho co-repressor. To define the mechanism of repression, we asked whether other co-repressors could interact with AML-1. We demonstrate that AML-1 interacts with the mSin3 co-repressors. Moreover, endogenous AML-1 associated with endogenous mSin3A in mammalian cells. A deletion mutant of AML-1 that did not interact with mSin3A failed to repress transcription. The AML-1/mSin3 association suggests a mechanism of repression for the chromosomal translocation fusion proteins that disrupt AML-1.  (+info)

Indispensable role of the transcription factor PEBP2/CBF in angiogenic activity of a murine endothelial cell MSS31. (2/119)

Mice lacking the AML1/PEBP2alphaB/CBFa2 gene or PEBP2beta/CBFb gene exhibit a defect in definitive hematopoiesis and die in utero because of hemorrhage in the central nervous system. Hematopoiesis in the embryo is considered to be tightly associated with vascular development. Here we examined whether PEBP2/CBF plays any role in angiogenesis besides that in definitive hematopoiesis. We found that AML1/PEBP2alphaB/CBFa2, PEBP2alphaA/CBFa1, and PEBP2beta/CBFb were expressed in a murine endothelial cell line MSS31. The expression of these molecules as well as the DNA binding activity of PEBP2/CBF were augmented by angiogenic growth factors such as bFGF and VEGF. Moreover, the expression of PEBP2 alpha/CBFa protein in endothelial cells was confirmed at the site of angiogenesis in vivo. To further clarify the role of PEBP2/CBF in angiogenesis, we established permanent transfectants of PEBP2 beta-MYH11 gene, one that interacts with the runt domain of the alpha subunit and deregulates PEBP2/CBF in a dominant interfering manner. Proliferation, migration, and tube formation of the PEBP2 beta-MYH11 transfectants were significantly reduced in comparison with those activities of the mock transfectants. These results suggest that transcription factor PEBP2/CBF plays an important role in angiogenesis.  (+info)

Biophysical characterization of interactions between the core binding factor alpha and beta subunits and DNA. (3/119)

Core binding factors (CBFs) play key roles in several developmental pathways and in human disease. CBFs consist of a DNA binding CBFalpha subunit and a non-DNA binding CBFbeta subunit that increases the affinity of CBFalpha for DNA. We performed sedimentation equilibrium analyses to unequivocally establish the stoichiometry of the CBFalpha:beta:DNA complex. Dissociation constants for all four equilibria involving the CBFalpha Runt domain, CBFbeta, and DNA were defined. Conformational changes associated with interactions between CBFalpha, CBFbeta, and DNA were monitored by nuclear magnetic resonance and circular dichroism spectroscopy. The data suggest that CBFbeta 'locks in' a high affinity DNA binding conformation of the CBFalpha Runt domain.  (+info)

Runt domain factor (Runx)-dependent effects on CCAAT/ enhancer-binding protein delta expression and activity in osteoblasts. (4/119)

Transcription factor CCAAT/enhancer-binding protein delta (C/EBPdelta) is normally associated with acute-phase gene expression. However, it is expressed constitutively in primary osteoblast cultures where it increases insulin-like growth factor I synthesis in a cAMP-dependent way. Here we show that the 3' proximal region of the C/EBPdelta gene promoter contains a binding sequence for Runt domain factor Runx2, which is essential for osteogenesis. This region of the C/EBPdelta promoter directed high reporter gene expression in osteoblasts, and specifically bound Runx2 in osteoblast-derived nuclear extract. C/EBPdelta gene promoter activity was reduced by mutating the Runx binding sequence or by co-transfecting with Runx2 antisense expression plasmid, and was enhanced by overexpression of Runx-2. Exposure to prostaglandin E(2) increased Runx-dependent gene transactivation independently of Runx2 binding to DNA. Runx2 bound directly to the carboxyl-terminal region of C/EBPdelta itself, and its ability to drive C/EBPdelta expression was suppressed when C/EBPdelta or its carboxyl-terminal fragment was increased by overexpression. Consistent effects also occurred on C/EBPdelta-dependent increases in gene expression driven by synthetic or insulin-like growth factor I gene promoter fragments. These interactions between Runx2 and C/EBPdelta, and their activation by prostaglandin E(2), provide new evidence for their importance during skeletal remodeling, inflammatory bone disease, or fracture repair.  (+info)

Energetic and functional contribution of residues in the core binding factor beta (CBFbeta ) subunit to heterodimerization with CBFalpha. (5/119)

Core-binding factors (CBFs) are a small family of heterodimeric transcription factors that play critical roles in several developmental pathways, including hematopoiesis and bone development. Mutations in CBF genes are found in leukemias and bone disorders. CBFs consist of a DNA-binding CBFalpha subunit (Runx1, Runx2, or Runx3) and a non-DNA-binding CBFbeta subunit. CBFalpha binds DNA in a sequence-specific manner, whereas CBFbeta enhances DNA binding by CBFalpha. Recent structural analyses of the DNA-binding Runt domain of CBFalpha and the CBFbeta subunit identified the heterodimerization surfaces on each subunit. Here we identify amino acids in CBFbeta that mediate binding to CBFalpha. We determine the energy contributed by each of these amino acids to heterodimerization and the importance of these residues for in vivo function. These data refine the structural analyses and further support the hypothesis that CBFbeta enhances DNA binding by inducing a conformational change in the Runt domain.  (+info)

Association with the nuclear matrix and interaction with Groucho and RUNX proteins regulate the transcription repression activity of the basic helix loop helix factor Hes1. (6/119)

Hairy/Enhancer of split 1 (Hes1) is a mammalian transcriptional repressor that plays crucial roles in the regulation of several developmental processes, including neuronal differentiation. The aim of this study was to elucidate the molecular mechanisms that regulate the transcription repression activity of Hes1. It is shown here that Hes1 associates with the nuclear matrix, the ribonucleoprotein network of the nucleus that plays important roles in transcriptional regulation. Nuclear matrix binding is mediated by the same Hes1 C-terminal domain that is also required for transcriptional repression. This domain contains the WRPW motif that acts as a binding site for the transcriptional corepressor Groucho, which also localizes to the nuclear matrix. Both the nuclear matrix association and transcription repression activity of Hes1 are inhibited by deletion of the WRPW motif, indicating that Groucho acts as a transcriptional corepressor for Hes1. This corepressor role is not modulated by the Groucho-related gene product Grg5. In contrast, the Runt-related protein RUNX2, which localizes to the nuclear matrix and interacts with Groucho and Hes1, can inhibit both the Groucho.Hes1 interaction and the transcription repression ability of Hes1. Together, these observations suggest that transcriptional repression by Hes1 requires interactions with Groucho at the nuclear matrix and that RUNX proteins act as negative regulators of the repressive activity of Groucho.Hes1 complexes.  (+info)

Cloning and embryonic expression patterns of the zebrafish Runt domain genes, runxa and runxb. (7/119)

We isolated zebrafish homologues of the Runt-related transcription factor gene family (Runx family), runxa and runxb, and analyzed their developmental expression patterns. The deduced amino acid sequence of Runxa was highly homologous to that of AML1 (also called CBFA2, PEBP2alphaB or Runx1), a critical regulator of mammalian hematopoiesis expressed in cells of the hematopoietic lineage as well as other tissues. During zebrafish development, the runxa gene was not expressed in hematopoietic tissues but in the olfactory placodes and cells attached to the otic vesicles. We identified three kinds of runxb transcripts, which encoded two types of proteins with different N-terminal regions. The Runxb proteins were highly similar to AML2 (CBFA3, PEBP2alphaC or Runx3). The expression sites of the shared region of runxb mRNAs during development were the trigeminal ganglions, dorsal neurons of the neural tube and the lateral line primordia. These findings show that expression patterns of the zebrafish Runx genes are distinct from that of the mammalian genes.  (+info)

Zebrafish homolog of the leukemia gene CBFB: its expression during embryogenesis and its relationship to scl and gata-1 in hematopoiesis. (8/119)

Mammalian CBFB encodes a transcription factor (CBF beta) that in combination with CBF alpha 2 binds to specific DNA sequences and regulates expression of a number of hematopoietic genes. CBFB is associated with human leukemias through a chromosome 16 inversion and is essential for definitive hematopoiesis during mouse embryo development. We have isolated a zebrafish cbfb complementary DNA (cDNA) clone from a zebrafish kidney cDNA library. This cbfb is highly homologous to human and mouse CBFB/Cbfb genes at both the DNA and protein level. In biochemical analyses, cbfbeta binds to human CBF alpha 2 and enhances its DNA binding. During zebrafish development, cbfb is expressed in the lateral plate mesoderm at tail bud stage and in the intermediate cell mass (ICM, the location of embryonic hematopoiesis) between the 21- to 26-somite stages. The cbfb is also expressed in Rohon-Beard cells, cranial nerve ganglia, hindbrain, retina, branchial arches, jaw, and fin buds. Expression of cbfb is decreased or absent in the ICM and Rohon-Beard cells in some hematopoietic mutants and is unaffected in others. We have also analyzed the expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors to cbfb in zebrafish hematopoiesis. Our results indicate that cbfb is expressed in early hematopoietic progenitors and that its expression pattern in the hematopoietic mutants is similar to that of scl. (Blood. 2000;96:4178-4184)  (+info)

BACKGROUND: Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously as oncoproteins and tumor suppressors to leukemia and other cancers. To discover fundamental Runx functions in the cell biology of animal development, we have employed morpholino antisense-mediated knockdown of the sea urchin Runx protein SpRunt-1. Previously we showed that embryos depleted of SpRunt-1 arrest development at early gastrula stage and underexpress the conventional protein kinase C SpPKC1. RESULTS: We report here that SpRunt-1 deficiency leads to ectopic cell proliferation and extensive apoptosis. Suppression of the apoptosis by pharmacological inhibition of caspase-3 prevents the ectopic proliferation and rescues gastrulation, indicating that many of the overt defects obtained by knockdown of SpRunt-1 are secondary to the apoptosis. Inhibition or knockdown of SpPKC1 also causes apoptosis, while cell survival is rescued in SpRunt-1 morphant embryos coinjected with
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There was an error published in Development 132, 4635-4644.. In Fig. 2, the incorrect image was shown in panel K. Owing to an error during figure assembly, Fig. 2K is a duplication of the wild-type embryo image shown in Fig. 5A. The corrected Fig. 2 appears below.. This error does not affect the conclusions of the paper. The authors apologise to readers for this mistake. ...
The mechanism underlying the lineage decision made by CD4(+)CD8(+) double-positive (DP) thymocytes that give rise to two T lymphocyte subset with distinct functionalities, that is, helper and cytotoxic T cells, remains a major issue in immunology. The lineage decision process involves several phases and terminates when cells loose their developmental plasticity to become the alternate lineage. A detailed picture of the transcription factor network governing helper versus cytotoxic-lineage decision has recently emerged. Studies published only past year provided new insights into how the expression of ThPOK, a central transcription factor for helper T cell development, is regulated. It has now become evident that an antagonistic interplay between ThPOK and Runx transcription factor complexes plays an essential role in thwarting an alternate fate during the commitment process.
Cell proliferation. To assess cell proliferation, 1 × 105 cells of the indicated AML-derived cells were seeded in 6-well plates. For the tetracycline-inducible gene or shRNA expression, doxycycline was added to the culture at a final concentration of 3 μM. Trypan blue dye exclusion assays were performed every other day.. RT-qPCR. Total RNA was isolated with an RNeasy Mini Kit (Qiagen) and reverse transcribed with a ReverTra Ace kit (TOYOBO) to generate cDNA. RT-qPCR was carried out with a 7500 Real-Time PCR System (Applied Biosystems) according to the manufacturers instructions. The results were normalized to GAPDH levels. Relative expression levels were calculated using the 2-ΔΔCt method. Primers used for RT-qPCR are listed in Supplemental Table 3.. ChIP-qPCR. ChIP was performed using a SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturers instructions. In brief, cells were cross-linked in 1% formaldehyde in PBS for 10 minutes at room ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
Runt-related transcription factor 1 (RUNX1) is generally considered to function as a tumor suppressor in the development of leukemia, but a growing body of evidence suggests that it has pro-oncogenic properties in acute myeloid leukemia (AML). Here we have demonstrated that the antileukemic effect mediated by RUNX1 depletion is highly dependent on a functional p53-mediated cell death pathway. Increased expression of other RUNX family members, including RUNX2 and RUNX3, compensated for the antitumor effect elicited by RUNX1 silencing, and simultaneous attenuation of all RUNX family members as a cluster led to a much stronger antitumor effect relative to suppression of individual RUNX members. Switching off the RUNX cluster using alkylating agent-conjugated pyrrole-imidazole (PI) polyamides, which were designed to specifically bind to consensus RUNX-binding sequences, was highly effective against AML cells and against several poor-prognosis solid tumors in a xenograft mouse model of AML without ...
Estrogen receptor α (ER α) and androgen receptor (AR) are master transcription factors in the breast and prostate, respectively. They are commonly known in development of sexual characteristics. However, both ERα and AR have been known to be involved in breast cancer (BCa) and prostate cancer (PCa) progression, respectively. The Runx family of transcription factors plays a role in hematopoiesis (Runx1), skeletogenesis (Runx2) and neurogenesis (Runx3). In addition, Runx proteins inhibit cell cycle progression, and have been assigned tumor suppressor roles in various contexts. Because both BCa and PCa cells metastasize to bone at high frequency, investigators have interrogated the possibility that they share characteristics with osteoblasts. Indeed, BCa and PCa cells were found to have osteomimetic properties, including expression of Runx2 and Runx2-target genes otherwise expressed by osteoblasts. Provoked by the reported physical interaction between AR and Runx2, we initiated a study to test ...
The Runx family of transcription factors supports cell fate determination, cell cycle regulation, global protein synthesis control, and genetic as well as epigenetic regulation of target genes. Runx1, which is essential for hematopoiesis; Runx2, which is required for osteoblast differentiation; and Runx3, which is involved in neurologic and gut development; are expressed in the growth plate during chondrocyte maturation, and in the chondrocytes of permanent cartilage structures. While Runx2 is known to control genes that contribute to chondrocyte hypertrophy, the functions of Runx1 and Runx3 during chondrogenesis and in cartilage tissue have been less well studied. The goals of this project were to characterize expression of Runx proteins in articular cartilage and differentiating chondrocytes and to determine the contribution of Runx1 to osteoarthritis (OA). Here, the expression pattern of Runx1 and Runx2 was characterized in normal bovine articular cartilage. Runx2 is expressed at higher levels in
Runx1 mediates the development of the granular convoluted tubules in the submandibular glands[1] The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription factor (RUNX) proteins (RUNX1, 2 and 3). This result clearly demonstrates that the Runx /Cbfb signaling pathway is indispensable in the development of the GCTs. However, it is not clear which of the RUNX proteins plays useful role in the development of the GCTs by activating the Runx /Cbfb signaling pathway. Past studies have revealed that the Runx /Cbfb signaling pathway plays important roles in various aspects of development and homeostatic events. Moreover, the Runx genes have different temporospatial ...
The Runt related transcription factors (RUNX) are recognized as key players in suppressing or promoting tumor growth. RUNX3, a member of this family, is known as a tumor suppressor in many types of cancers, although such a paradigm was challenged by some researchers. The TGF-β pathway governs major upstream signals to activate RUNX3. RUNX3 protein consists of several regions and domains. The Runt domain is a conserved DNA binding domain and is considered as the main part of RUNX proteins since. Herein, we compared the effects of Runt domains and full-Runx3 in cell viability by designing two constructs of Runx3, including N-terminal region and Runt domain. We investigated the effect of full-Runx3, N-t, and RD on growth inhibition in AGS, MCF-7, A549, and HEK293 cell lines which are different in TGF-β sensitivity, in the absence and presence of TGF-β. The full length RUNX3 did not notably inhibit growth of these cell lines while, the N-t and RD truncates showed different trends in these cell lines.
Complete information for CBFB gene (Protein Coding), Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Looking for online definition of core-binding factor, runt domain, alpha subunit 1 in the Medical Dictionary? core-binding factor, runt domain, alpha subunit 1 explanation free. What is core-binding factor, runt domain, alpha subunit 1? Meaning of core-binding factor, runt domain, alpha subunit 1 medical term. What does core-binding factor, runt domain, alpha subunit 1 mean?
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Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. Few prognostic markers have been identified. In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. Overall, 10-year overall survival (OS), disease-free survival (DFS), and event-free survival were 63.9%, 54.8%, and 49.9%, respectively; patients with the t(8;21) and inv(16) chromosomal rearrangements exhibited significant differences at diagnosis. Despite similarly high complete remission (CR) rate, patients with inv(16) experienced superior DFS and a high chance of achieving a second CR, often leading to prolonged OS also after relapse. We found that a complex karyotype (ie, ≥4 cytogenetic anomalies) affected survival; the KIT D816 mutation predicted worse prognosis only in patients with the t(8;21) rearrangement, whereas FLT3 mutations had no ...
Yano et al show that TD-198946 strongly induced in vitro chondrogenic differentiation of progenitors, as evidenced by a marked increase in the chondrogenic markers type II collagen and aggrecan; it did this without promoting hypertrophy (type X collagen and metalloprotease 13).8. This interesting balance between chondrogenesis and hypertrophic differentiation may be because TD-198946 exerts its effect through regulating the expression of Runx1, a known inducer of chondrogenic differentiation and a suppressor of the subsequent hypertrophy.10 The mammalian Runx protein family comprises three transcription factors: Runx1, Runx2 and Runx3. All Runx proteins are expressed during chondrogenesis; however, experimental results show a distinct role for Runx proteins in chondrogenesis and subsequent chondrocyte maturation.12 ,13 Runx1 is highly expressed during chondrogenesis in comparison with Runx2. Overall, the expression of Runx1 remained significantly higher than Runx2 expression during early limb ...
The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g., RUNX1) and osteogenesis (e.g., RUNX2). The beta subunit is a non-DNA binding regulatory subunit; it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. Alternative splicing generates two mRNA variants, each encoding a distinct carboxyl terminus. In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. This chromosomal rearrangement is associated with acute myeloid leukemia of the M4Eo subtype. Two ...
Background. Members of the Runx gene family encode transcription factors that bind to DNA in a sequence-specific manner. Among the three Runx proteins, Runx2 comprises 607 amino acid (aa) residues, is expressed in bone, and plays crucial roles in osteoblast differentiation and bone development. We examined whether the Runx2 gene is also expressed in testes. Methods. Murine testes from 1-, 2-, 3-, 4-, and 10-week-old male mice of the C57BL/6J strain and W∕Wv strain were used throughout the study. Northern Blot Analyses were performed using extracts form the murine testes. Sequencing of cDNA clones and 5′-rapid amplification of cDNA ends were performed to determine the full length of the transcripts, which revealed that the testicular Runx2 comprises 106 aa residues coding novel protein. Generating an antiserum using the amino-terminal 15 aa of Runx2 (Met1 to Gly15) as an antigen, immunoblot analyses were performed to detect the predicted polypeptide of 106 aa residues with the initiating Met1. With
The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
Decreased Llgl1 expression has been previously shown to be associated with increased metastatic potential, malignant phenotype, or inferior survival in a variety of solid tumors (Ohali et al., 2004; Schimanski et al., 2005; Kuphal et al., 2006). Given that Llgl1 restrains self-renewal in HSCs and that leukemia is characterized by aberrant activation of self-renewal, we assessed for a role in human AML. First, we asked whether the gene expression signature revealed by genetic inactivation of Llgl1 (Llgl1−/− signature) in HSCs was associated with any genetic subtypes of primary AML. Indeed, clustering based on the Llgl1−/− signature (Fig. 4 B) grouped AML cases into subgroups significantly associated with cytogenetic abnormalities (Fig. 4, B and C). Especially core-binding factor leukemias (t(8;21) and inv(16)) or t(15;17) clustered into specific subgroups. Moreover, the Llgl1−/− signature was most strongly correlated with group 7 based on hierarchical clustering. This group contained ...
Acute myeloid leukemia 2 protein antibody, Acute myeloid leukemia gene 2 antibody, AML 2 antibody, AML2 antibody, CBF alpha 3 antibody, CBF-alpha-3 antibody, CBFA 3 antibody, CBFA3 antibody, Core binding factor alpha 3 subunit antibody, core binding factor antibody, Core binding factor runt domain alpha subunit 3 antibody, Core binding factor subunit alpha 3 antibody, core-binding factor antibody, Core-binding factor subunit alpha-3 antibody, FLJ34510 antibody, MGC16070 antibody, Oncogene AML 2 antibody, Oncogene AML-2 antibody, PEA2 alpha C antibody, PEA2-alpha C antibody, PEBP2 alpha C antibody, PEBP2-alpha C antibody, Pebp2a3 antibody, PEBP2aC antibody, Polyomavirus enhancer binding protein 2 alpha C subunit antibody, Polyomavirus enhancer-binding protein 2 alpha C subunit antibody, runt domain alpha subunit 3 antibody, runt related transcription factor 3 antibody, Runt-related transcription factor 3 antibody, RUNX 3 antibody, Runx3 antibody, RUNX3_HUMAN antibody, SL3 3 enhancer factor 1 ...
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This study is examining the appropriate dose and side effects of dasatinib, when it is given with the standard of care chemotherapy for children and adolescents
TY - JOUR. T1 - New challenges in integrated diagnosis by imaging and osteo-immunology in bone lesions. AU - Schiano, Concetta. AU - Soricelli, Andrea. AU - de Nigris, Filomena. AU - Napoli, Claudio. PY - 2018/12/20. Y1 - 2018/12/20. N2 - INTRODUCTION: High-resolution imaging is the gold standard to measure the functional and biological features of bone lesions. Imaging markers have allowed the characterization both of tumour heterogeneity and metabolic data. Besides, ongoing studies are evaluating a combined use of imaging markers, such as SUVs, MATV, TLG, ADC from PET and MRI techniques respectively, and several biomarkers spanning from chemokine immune-modulators, such as PD-1, RANK/RANKL, CXCR4/CXCL12 to transcription factors, such as TP53, RB1, MDM2, RUNX family, EZH2, YY1, MAD2. Osteoimmunology may improve diagnosis and prognosis leading to precision medicine in bone lesion treatment. Areas covered: We investigated modalities (molecular and imaging approach) useful to identify bone ...
A laminated structure comprising, at least one optically anisotropic layer formed of a liquid crystalline composition comprising a compound having two or more types of reactive groups, and at least one photosensitive polymer layer. The laminated structure is useful for forming an optically anisotropic layer inside of a liquid crystal cell. The laminated structure is also useful for forming a liquid crystal cell substrate with an optically anisotropic layer having an optically compensating ability, inside of a liquid crystal cell.
Among patients with good prognosis core binding factor AML, there is an overall survival rate of only 44%. To understand the genetic factors contributing to poor outcomes within this subgroup, Dr Chew is analysing bone marrow samples collected from 18 patients before and during treatment.. According to Dr Chew, multiple genetic abnormalities acquired during therapy are probably responsible for good prognosis core binding AML developing resistance to chemotherapy.. To help us predict who will respond poorly to therapy, were identifying the genetic mutations occurring in patients who relapse, he said. This information will allow us to tailor patient treatment accordingly. Currently a stem cell transplant is considered the definitive treatment and our findings will help clinicians decide if their patients AML will develop resistance and if a stem cell transplant is recommended.. Dr Chew is testing the usefulness of the genetic variations he identifies through an international ...
RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding…
TY - JOUR. T1 - Combination of dasatinib with chemotherapy in previously untreated core binding factor acute myeloid leukemia. T2 - CALGB 10801. AU - Marcucci, Guido. AU - Geyer, Susan. AU - Laumann, Kristina. AU - Zhao, Weiqiang. AU - Bucci, Donna. AU - Uy, Geoffrey L.. AU - Blum, William. AU - Eisfeld, Ann Kathrin. AU - Pardee, Timothy S.. AU - Wang, Eunice S.. AU - Stock, Wendy. AU - Kolitz, Jonathan E.. AU - Kohlschmidt, Jessica. AU - Mrózek, Krzysztof. AU - Bloomfield, Clara D.. AU - Stone, Richard M.. AU - Larson, Richard A.. N1 - Publisher Copyright: © 2020 by The American Society of Hematology. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.. PY - 2020/2/25. Y1 - 2020/2/25. N2 - Acutemyeloid leukemia (AML)witheither t(8;21)(q22;q22)or inv(16)(p13q22)/t(16;16)(p13;q22) is referred to as core binding factor (CBF) AML. Although categorized as favorable risk, long-term survival for these patients is only ∼50% to 60%. Mutated (mut) or overexpressed KIT, a gene encoding a ...
To study the effects and importance of fluoride on FBs in the development of extraperiosteal calcification and the ossification of skeletal fluorosis, the presence of the osteogenic phenotype, which is indicated by the expression of core-binding factor a1 (Cbfa1) and osteocalcin (OCN), in an FB cell line (L929) and in osteoblasts (OBs) exposed to fluoride was determined. Fibroblasts and osteoblasts were exposed to different concentrations of fluoride (0, 0.0001, 0.001, 0.1, 1.0, 10.0 and 20.0mg/L F-). By using RT-PCR and ELISA, the mRNA levels of Cbfa1 and OCN were measured at 48h, and the protein levels of Cbfa1 and OCN were measured at 2, 4, 24, 48 and 72h. The data demonstrated the following: (1) The Cbfa1 protein level in fluoride-treated fibroblasts clearly increased at 48h in the groups treated with 0.0001, 0.001, 0.1, 1.0 and 20.0mg/L F-. The Cbfa1 protein level of the group treated with 10mg/L F- at 72h was higher than that of the control group. The level of Cbfa1 mRNA in the fibroblasts ...
Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. Runx proteins are encoded by three genes: Runx1, Runx2 , and ...
The pathogenesis of PDAC involves genetic alterations, such as K-ras protooncogene mutations, mutations of the p53, p16, and Smad4 tumour suppressor genes, and other less common mutations.2 In addition, there are numerous epigenetic alterations, including altered expression of several growth factors and their receptors.3 For example, PDACs overexpress all TGFβ isoforms and their receptors, and overexpression of these ligands and receptors is often associated with shortened postoperative survival of patients with pancreatic cancer.14,15. The TGFβ pathway is carefully regulated, with Smad proteins as the key component in the signal transduction pathway. In addition, other regulators, such as transcription factors, which facilitate Smad binding to target promoters, may provide routes for feedback and crosstalk.16 For example, members of the CBFA (core binding factor A) family of transcription factors act both as targets and partners of activated Smads. This family, also termed the Runx family, ...
Cells, Lead, Acute Myeloid Leukemia, Bone, Bone Marrow, Core-binding Factor, Cytogenetic, Cytogenetic Abnormalities, Disease, Leukemia, Marrow, Myeloid Leukemia, Patient, Patients, Protein Array, Proteins, Regression, Regression Analysis, Ability, Algorithm
Veteranorientering i nordvästra Skåne är en samling härliga människor som orienterar tillsammans varannan onsdag. Arrangemanget går runt på veteraner i de olika klubbarna i nordvästra Skåne och det är alltid start klockan 09.00. Varmt välkommen att bli en av oss ...
TY - JOUR. T1 - HDAC1 is a required cofactor of CBFb-SMMHC and a potential therapeutic target in inversion 16 acute myeloid leukemia. AU - Richter, Lisa E.. AU - Wang, Yiqian. AU - Becker, Michelle E.. AU - Coburn, Rachel A.. AU - Williams, Jacob T.. AU - Amador, Catalina. AU - Hyde, R. Katherine. PY - 2019/6/1. Y1 - 2019/6/1. N2 - Acute myeloid leukemia (AML) is a neoplastic disease characterized by the uncontrolled proliferation and accumulation of immature myeloid cells. A common mutation in AML is the inversion of chromosome 16 [inv (16)], which generates a fusion between the genes for core binding factor beta (CBFB) and smooth muscle myosin heavy chain gene (MYH11), forming the oncogene CBFB-MYH11. The expressed protein, CBFb-SMMHC, forms a heterodimer with the key hematopoietic transcription factor RUNX1. Although CBFb-SMMHC was previously thought to dominantly repress RUNX1, recent work suggests that CBFb-SMMHC functions together with RUNX1 to activate transcription of specific target ...
Runt-related transcription factor 1 (RUNX1) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters and can accelerate apoptosis in various tumors. However, the regulatory mechanisms underlying RUNX1 expression in neuroblastoma (NB), a highly malignant tumor in childhood, remain largely unclear. In this study, we aimed to assess the role of RUNX1 in NB and to reveal the underlying mechanisms that may contribute to finding a potential therapeutics strategy against NB. Growth, invasion, metastasis and angiogenesis were assessed using Cell Counting Kit-8 (CCK-8) immunocytochemistry, and studies involving soft agar, cell invasion, tube formation and whole animals. The levels of expression were measured using real-time quantitative PCR for RNA, Western blot and immunostaining analyses for proteins. Luciferase reporter and chromatin immunoprecipitation assays indicated that RUNX1 directly binds within the BIRC5, CSF2RB and NFKBIA promoter regions to facilitate
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RUNX1 antibody (runt-related transcription factor 1) for WB. Anti-RUNX1 pAb (GTX11903) is tested in Human, Mouse samples. 100% Ab-Assurance.
RUNX1 antibody (runt-related transcription factor 1) for ICC/IF, WB. Anti-RUNX1 pAb (GTX129100) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
TY - JOUR. T1 - Loss of runt-related transcription factor 3 induces gemcitabine resistance in pancreatic cancer. AU - Horiguchi, Shigeru. AU - Shiraha, Hidenori. AU - Nagahara, Teruya. AU - Kataoka, Jyunnro. AU - Iwamuro, Masaya. AU - Matsubara, Minoru. AU - Nishina, Shinichi. AU - Kato, Hironari. AU - Takaki, Akinobu. AU - Nouso, Kazuhiro. AU - Tanaka, Takehiro. AU - Ichimura, Koichi. AU - Yagi, Takahito. AU - Yamamoto, Kazuhide. PY - 2013/8. Y1 - 2013/8. N2 - Background & Aim: Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene that is expressed in gastric and other cancers including pancreatic cancer. However, the precise function of RUNX3 in pancreatic cancer has not been fully elucidated. In this study, we aimed to determine the effect of decreased RUNX3 expression in pancreatic cancer. Methods: This study included 36 patients with primary pancreatic cancer, who had undergone pancreaticoduodenectomy. All patients were treated with 1000mg/m2 gemcitabine after the surgery. ...
A temperature compensation circuit arrangement for liquid crystal cells in optical devices is presented. In an optical device, a liquid crystal cell typically manipulates the optical signals according to an output optical property, such as attenuation, responsive to an AC voltage source electrical signal. A feedback circuit arrangement is connected to the liquid crystal cell and controls the current through the liquid crystal cell with respect to temperature by a predetermined control equations for the output optical property so that the device manipulates the optical signals independently of temperature. The current follows the control equations, which are empirically determined with respect to temperature for one equation.
Core-binding factors (CBFs) are a small family of transcription factors that play important roles in several developmental processes and in human disease. CBFs consist of CBFα (Runx1, Runx2, or Runx3) and CBFβ. The CBFα subunit binds DNA in a ...
Ustun C, Morgan E, Moodie EEM, Pullarkat S, Yeung C, Broesby-Olsen S, Ohgami R, Kim Y, Sperr W, Vestergaard H, Chen D, Kluin PM, Dolan M, Mrózek K, Czuchlewski D, Horny HP, George TI, Kristensen TK, Ku NK, Yi CA, Møller MB, Marcucci G, Baughn L, Schiefer AI, Hilberink JR, Pullarkat V, Shanley R, Kohlschmidt J, Coulombe J, Salhotra A, Soma L, Cho C, Linden MA, Akin C, Gotlib J, Hoermann G, Hornick J, Nakamura R, Deeg J, Bloomfield CD, Weisdorf D, Litzow MR, Valent P, Huls G, Perales MA, Borthakur G Core-binding factor acute myeloid leukemia with t(8;21): Risk factors and a novel scoring system (I-CBFit). View on PubMed ...
Ve vojensk m oble en , s kovovou helmou na hlav a atrapami v bu nin se fanou ek Falloutu proch zel centrem m sta, jako by se nechumelilo.
Stem Cells International is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of stem cell biology and applications. The journal will consider basic, translational, and clinical research, including animal models and clinical trials.
Disease, Muscle, Muscle Cells, Smooth Muscle, Smooth Muscle Cells, Cells, Heme, Hydrogen, Alkaline Phosphatase, Kidney, Kidney Disease, Osteocalcin, Upregulation, Vascular Calcification, Calcium, Heme Oxygenase, Vascular Smooth Muscle, Atherosclerosis, Bone, Core Binding Factor
van der Deen M, Taipaleenmaki H, Zhang Y, Teplyuk NM, Gupta A, Cinghu S, Shogren K, Maran A, Yaszemski MJ, Ling L, Cool SM, Leong DT, Dierkes C, Zustin J, Salto-Tellez M, Ito Y, Bae SC, Zielenska M, Squire JA, Lian JB, Stein JL, Zambetti GP, Jones SN, Galindo M, Hesse E, Stein GS, van Wijnen AJ. MicroRNA-34c inversely couples the biological functions of the runt-related transcription factor RUNX2 and the tumor suppressor p53 in osteosarcoma. J Biol Chem. 2013 Jul 19; 288(29):21307-19. Epub 2013 May 29 ...
1LJM: DNA Recognition by the RUNX1 Transcription Factor Is Mediated by an Allosteric Transition in the RUNT Domain and by DNA Bending.
Everything Else Addalaichenai, Website: https://www.teyuchiller.com SKYPE: teyuchiller Email: [email protected] Tel: + 86-20-89301885 Fax: + 86-20-...
KIT gene mutations occur in approximately 25% of cases of acute myeloid leukemia (AML) with t(8;21) or inv(16), also referred to as core-binding factor AML. These mutations are predominately small length affecting mutations in KIT exon 8 and substitution mutations in KIT exon 17. KIT mutations in core-binding factor AML may be an adverse prognostic factor in this otherwise favorable-risk disease. This DNA sequencing test detects mutations in exons 8 and 17 of the KIT gene in blood and bone marrow specimens. This test will detect mutations in specimens when at least 30% of the nucleated cells carry the mutation.. ...
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... subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, ... RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa, a beta (β) ... The core enzyme has five subunits (~400 kDa):[23] *β': The β' subunit is the largest subunit, and is encoded by the rpoC gene.[ ... In order to bind promoters, RNAP core associates with the transcription initiation factor sigma (σ) to form RNA polymerase ...
The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) subunits) binds a sigma ... Sigma factors in E. coli:. *σ70(RpoD) - σA - the "housekeeping" sigma factor or also called as primary sigma factor, ... Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... Different sigma factors are utilized under different environmental conditions. These specialized sigma factors bind the ...
Chen Y; Le Cahérec F; Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. GRCh38: ... Knight BC; High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ...
DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... "New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ...
... sandwiched between the Walker A and B motifs of one subunit and the LSGGQ motif of the other subunit. The maltose binding ... heme-binding protein, and alkaline protease), heme, hydrolytic enzymes, S-layer proteins, competence factors, toxins, ... In addition, a gap in the protein is accessible directly from the hydrophobic core of the inner leaflet of the membrane bilayer ... The T domains are each built of typically 10 membrane spanning alpha helices, through which the transported substance can cross ...
Törö I, Thore S, Mayer C, Basquin J, Séraphin B, Suck D (May 2001). "RNA binding in an Sm core domain: X-ray structure and ... RNA oligonucleotides generally bind inside the hole (lumen) of the LSm torus, one nucleotide per LSm subunit, but additional ... The short (two to four turns) N-terminal alpha helix occurs at one edge of the beta sandwich. This alpha helix and the beta ... the Sm-like protein HF-I encoded by the gene hfq was described in 1968 as an essential host factor for RNA bacteriophage Qβ ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
... factor implicated in the tumorigenesis and progression of breast cancer via its binding to the estrogen receptor alpha (ERα). ... Further, HATs have diverse role as transcription factors beside having histone acetylase activity, e.g., HAT subunit, hADA3 may ... The transcriptional regulation of the genome is controlled primarily at the preinitiation stage by binding of the core ... Epigenetics Histone Nucleosomes Chromatin Histone acetyltransferase Transcription factors CAF-1 (Chromatin assembly factor-1 ...
Zipper Coiled-Coil Domains of the cGMP-Dependent Protein Kinase I alpha and its Interaction with the Myosin Binding Subunit of ... Leucine zipper coiled-coil helices & DNA-binding helices: transcription factor Max (PDB file 1HLO) ... in the hydrophobic core, and one containing predominantly polar amino acids oriented toward the solvent-exposed surface of the ... DNA bindingEdit. α-Helices have particular significance in DNA binding motifs, including helix-turn-helix motifs, leucine ...
These core histones are rich in lysine and arginine residues. The carboxyl (C) terminal end of these histones contribute to ... It results in good optimization and is used in vivo to reveal DNA-protein binding occurring in cells. ChIP-Seq can be used to ... H3K9ac indicates acetylation of lysine 9 on histone H3 protein subunit: The genomic DNA of eukaryotic cells is wrapped around ... Maruta H, Greer K, Rosenbaum JL (1986). "The acetylation of alpha-tubulin and its relationship to the assembly and disassembly ...
sequence-specific DNA binding. Cellular component. • PML body. • Нуклеоплазма. • DNA replication factor A complex. • nuclear ... Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". EMBO J. 21 (7): 1855-63. ... The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". J. Biol. Chem. 273 (7): 3932-6 ... Dornreiter I, Erdile LF, Gilbert IU, von Winkler D, Kelly TJ, Fanning E (1992). „Interaction of DNA polymerase alpha-primase ...
An important factor in the binding of the first generation NNRTIs, such as nevirapine, is the butterfly-like shape. Despite ... The cloroindole part interacted with the hydrophobic core of the pocket and influenced the binding mode of the R120393 so it ... Part 1: From alpha-anilinophenylacetamide (alpha-APA) to imidoyl thiourea (ITU)". Bioorganic & Medicinal Chemistry Letters. 11 ... Å from the catalytic site in the palm domain of the p66 subunit site of the enzyme. The NNRTI binding pocket (NNIBP) contains ...
Diagram of the 12 subunit catalytic core of the cubic 24 subunit human E2k. [18] (C) Ribbon diagram of the 8 Monomer E3 subunit ... File:Structure of alpha-KGDHC.jpg Figure 7: Structure of the alpha-KGDHC subunits (A) Ribbon diagram of the homo-dimer ... The first factor was a decrease in transcription (mRNA levels) of the subunits comprising α-KGDHC. As shown in Figure 13, after ... It is an 8 monomer that can be divided into 4 domains; the FAD-binding domain (residues 1-149), the NAD+-binding domain ( ...
For example, a protein called eukaryotic initiation factor-2 (eIF-2) can bind to the smaller subunit of the ribosome, starting ... The two reacting molecules are the alpha amino group of one amino acid and the alpha carboxyl group of the other amino acids. A ... In a hydrophilic environment such as cytosol, the hydrophobic amino acids will concentrate at the core of the protein, while ... These subunits surround the mRNA strand. The larger subunit contains three binding sites: A (aminoacyl), P (peptidyl), and E ( ...
BDNF is made in the endoplasmic reticulum and secreted from dense-core vesicles. It binds carboxypeptidase E (CPE), and the ... regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits ... receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. Cellular component. • cytoplasm. • ... BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor. ...
Each AMPAR has four sites to which an agonist (such as glutamate) can bind, one for each subunit.[5] The binding site is ... Armstrong N, Sun Y, Chen GQ, Gouaux E (October 1998). "Structure of a glutamate-receptor ligand-binding core in complex with ... "Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin- ... "Postsynaptic factors in the expression of long-term potentiation (LTP): increased glutamate receptor binding following LTP ...
positive regulation of core promoter binding. • interleukin-12-mediated signaling pathway. • regulation of regulatory T cell ... positive regulation of tumor necrosis factor production. • CD8-positive, alpha-beta T cell differentiation involved in immune ... including subunits of the immunoproteasome (MECL1, LMP2, LMP7), as well as TAP and ERAAP in addition possibly to the direct ... However, in contrast to many other heparan sulfate binding proteins, where binding promotes biological activity, the binding of ...
... binding studies reveal DNA binding specificity mechanisms and functional interplay amongst Forkhead transcription factors". ... POLD1, CDC2, CRCS10, MDPL, POLD, polymerase (DNA) delta 1, catalytic subunit, DNA polymerase delta 1, catalytic subunit. ... The major replicative activity in S phase of cell cycle depends on three DNA polymerases - Polymerase alpha (Polα), Polymerase ... The light blue boxes represent interactions of the core complex. The light pink boxes represent other putative interactions in ...
... a 9-subunit base that binds directly to the α ring of the 20S core particle, and a 10-subunit lid. Six of the nine base ... Degradation of Aux/IAA proteins derepresses transcription factors in the auxin-response factor (ARF) family and induces ARF- ... which serve as docking domains for the regulatory particles and the alpha subunits N-termini (Pfam PF10584) form a gate that ... The mechanisms by which it binds to the core particle through the C-terminal tails of its subunits and induces α-ring ...
ubiquitin protein ligase binding. • importin-alpha family protein binding. • disordered domain specific binding. • core ... DNA binding. • core promoter binding. • transcription factor activity, sequence-specific DNA binding. • transcription ... Within each subunit, there are a variety of protein binding sites, as well as a total of 15 possible phosphorylation sites. ... transcription factor binding. • RNA polymerase II activating transcription factor binding. • phosphoprotein binding. • kinase ...
phosphopantetheine binding. • 3-oxo-pimeloyl-[acp methyl ester reductase activity]. • RNA binding. • cadherin binding. • 3-oxo- ... In some cancer cell lines, this protein has been found to be fused with estrogen receptor alpha (ER-alpha), in which the N- ... Reevaluation of the side-by-side, antiparallel subunit model". J. Biol. Chem. 274 (17): 11557-63. doi:10.1074/jbc.274.17.11557 ... Metabolism and homeostasis of fatty acid synthase is transcriptionally regulated by Upstream Stimulatory Factors (USF1 and USF2 ...
This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding ... C1QBP has been shown to interact with Protein kinase D1,[10] BAT2,[11] PRKCD,[10] PKC alpha[10] and Protein kinase Mζ.[10] ... "Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation". J Clin ... transcription factor binding. • protein binding. • hyaluronic acid binding. • kininogen binding. • mitochondrial ribosome ...
... being bound by several eukaryotic initiation factors, including eIF1, eIF1A, and eIF3.[6] The 40S ribosomal subunit is also ... This rRNA core is decorated with dozens of proteins. In the figure "Crystal Structure of the Eukaryotic 40S Ribosomal Subunit ... Eukaryote-specific extensions of conserved proteins, ranging from a few residues or loops to very long alpha helices and ... Eukaryotic large ribosomal subunit (60S). References[edit]. *^ 40S+Ribosomal+Subunits at the US National Library of Medicine ...
"Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". EMBO J. (England) 21 (7): ... "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain.". J. Biol. Chem. 273 (7): 3932- ... "Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.". EMBO J. 11 (2): 769-76. ... "An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair." ...
... the position where the substrate binds, may influence factors like binding affinity of ligands, stabilization of substrates ... The number of sub-units making up cellulosomes can also determine the rate of enzyme activity.[12] ... McCleary BV (November 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ... construction of the catalytic core, expression, and X-ray structure at 1.75 Å resolution". Biochemistry. 36 (51): 16032-9. doi: ...
transcription factor activity, sequence-specific DNA binding. • transcription factor binding. • RNA polymerase II core promoter ... 2009). "The correlation of hepatocyte nuclear factor 4 alpha and 3 beta with hepatitis B virus replication in the liver of ... "Foxa2 and MafA regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression". J. Mol. ... transcription regulatory region DNA binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. ...
"Lipopolysaccharide-binding protein plasma levels and liver TNF-alpha gene expression in obese patients: evidence for the ... Core[edit]. Main article: Core oligosaccharide. The core domain always contains an oligosaccharide component that attaches ... LPS final assembly: O-antigen subunits are translocated across the inner membrane (by Wzx) where they are polymerized (by Wzy, ... The skin can show the effects of vascular damage often coupled with depletion of coagulation factors in the form of petechiae, ...
... two subunits are bound during each conformational change, whereas for tubulin binding takes place with four subunits. Actin has ... alpha skeletal muscle actin in the nuclei of mouse myoblasts,[111]. *cytoplasmic gamma actin and also alpha smooth muscle actin ... Nucleating factors are necessary to stimulate actin polymerization. One such nucleating factor is the Arp2/3 complex, which ... Type VI and VIIa myosins are present throughout this core, while myosin XVa is present in its extremities in quantities that ...
The alpha helical anticodon binding domain of Arginyl, Glycyl and Cysteinyl-tRNA synthetases is known as the DALR domain after ... Alignment of the core domains of aminoacyl-tRNA synthetases class I and class II. Essential binding site residues (Backbone ... It aminoacylates at the 3'-OH of a terminal adenosine on tRNA, and is usually dimeric or tetrameric (two or four subunits, ... and an anticodon binding domain (which interacts mostly with the anticodon region of the tRNA and ensures binding of the ...
Assembly factor proteins[31] 47. NDUFAF1c. CIA30_HUMAN. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex, assembly factor 1. ... Core Subunitsa 1. NDUFS7 / PSST / NUKM. NDUS7_HUMAN. NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial EC ... Complex I contains a ubiquinone binding pocket at the interface of the 49-kDa and PSST subunits. Close to iron-sulfur cluster ... 1 alpha subcomplex subunit 3. Pfam PF14987 39. NDUFA4 / MLRQc. NDUA4_HUMAN. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex ...
... caused by the alpha decay of actinium-227.[35] Perey then attempted to determine the proportion of beta decay to alpha decay in ... Gottschlich, Michele M. (2001). The Science and Practice of Nutrition Support: A Case-based Core Curriculum. Kendall Hunt. p. ... The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... Solid phenyllithium forms monoclinic crystals can be described as consisting of dimeric Li2(C6H5)2 subunits. The lithium atoms ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
protein kinase binding. • core promoter binding. • RNA polymerase II transcription coactivator activity. • transcription factor ... ribosomal large subunit binding. • protein homodimerization activity. • ribosomal small subunit binding. • unfolded protein ... regulation of eIF2 alpha phosphorylation by dsRNA. • positive regulation of cell cycle G2/M phase transition. • negative ... activating transcription factor binding. • rRNA binding. • protein N-terminus binding. • chromatin binding. ...
BDNF is made in the endoplasmic reticulum and secreted from dense-core vesicles. It binds carboxypeptidase E (CPE), and the ... regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits ... receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ... BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor. ...
... s can bind to other proteins as well as to small-molecule substrates. When proteins bind specifically to other copies of ... Kauzmann W (May 1956). "Structural factors in protein denaturation". Journal of Cellular Physiology. 47 (Suppl 1): 113-31. doi: ... Quaternary structure: the structure formed by several protein molecules (polypeptide chains), usually called protein subunits ... The membrane alone has a hydrophobic core through which polar or charged molecules cannot diffuse. Membrane proteins contain ...
... the alpha helix). Linus Pauling was the first to identify[29] the 3.6 amino acids per helix turn ratio of the alpha helix. ... It, however, requires a five base binding between the mRNA and tRNA with a flip of the anti-codon creating a triplet coding, ... Such a code might be "degenerate", with 4×4×4=64 possible triplets of the four nucleotide subunits while there were only 20 ... In 1960, Crick accepted an honorary fellowship at Churchill College, Cambridge, one factor being that the new college did not ...
Each tropomyosin molecule has a smaller calcium-binding protein called troponin bound to it. All thin filaments are attached to ... The filaments are organized into repeated subunits along the length of the myofibril. These subunits are called sarcomeres. ... They run through the core of each thick filament and anchor it to the Z-line, the end point of a sarcomere. Titin also ... Troponin and the associated tropomyosin undergo a conformational change after calcium binding and expose the myosin binding ...
Each subunit consists of three domains. At the carboxyl terminal of the peptide chain there's a short alpha helix domain that ... oxygen binding. • metal ion binding. • dopamine binding. • enzyme binding. • oxidoreductase activity. • iron ion binding. • ... response to growth factor. • response to ethanol. • cellular response to glucose stimulus. • phthalate metabolic process. • ... allows tetramerization.[15] The central ~300 amino acids make up a catalytic core, in which all the residues necessary for ...
... is the gene encoding the ε subunit of DNA polymerase III in Escherichia coli.[1] The ε subunit is one of three core ... of the gene product binds the θ subunit and carries out the exonuclease function and the C-terminus binds the α subunit ... "The proofreading exonuclease subunit epsilon of Escherichia coli DNA polymerase III is tethered to the polymerase subunit alpha ... The ε subunit is stabilized by the θ subunit within the complete polymerase complex.[7] ...
14 alpha particles) is easily produced from lighter nuclei in the alpha process in nuclear reactions in supernovae (see silicon ... The inner core of the Earth is generally presumed to consist of an iron-nickel alloy with ε (or β) structure.[10] ... Hemoglobin bound to carbon monoxide is known as carboxyhemoglobin. This effect also plays a minor role in the toxicity of ... The ensuing availability of inexpensive iron was one of the factors leading to the Industrial Revolution. Toward the end of the ...
InaD contains five binding domains called PDZ domain proteins, which specifically bind the C termini of target proteins. ... Drosophila differentially expresses a core group of 252 genes upon infection with most bacteria. This core group of genes is ... Bakker K (1961). "An analysis of factors which determine success in competition for food among larvae of Drosophila ... A 2016 study concluded that food supplementation with 10-mM alpha-ketoglutarate decreased Drosophila alcohol sensitivity over ...
The activated Gs alpha subunit binds to and activates an enzyme called adenylyl cyclase, which, in turn, catalyzes the ... crystallised the PKA Cα subunit, which revealed the bi-lobe structure of the protein kinase core for the very first time, ... Long term memory is dependent on the CREB transcription factor, regulated by PKA. A study done on drosophila reported that an ... Two cAMP molecules bind to each PKA regulatory subunit. *The regulatory subunits move out of the active sites of the catalytic ...
In the presence of the R subunit, the complex can also act as an endonuclease, binding to the same target sequence but cutting ... A conserved enzyme core with variable target-recognizing domains". J. Mol. Biol. 206 (2): 305-12. doi:10.1016/0022-2836(89) ... hsdM contains an alpha-helical domain at the N-terminus, the HsdM N-terminal domain.[7] ... The type I restriction and modification system is composed of three polypeptides R, M and S. The M (hsdM) and S subunits ...
Most vegetable oils are rich in linoleic acid (safflower, sunflower, and corn oils). Alpha-linolenic acid is found in the green ... Malinauskas T (2008). "Docking of fatty acids into the WIF domain of the human Wnt inhibitory factor-1". Lipids. 43 (3): 227-30 ... The steroids, all derived from the same fused four-ring core structure, have different biological roles as hormones and ... Polyketides are synthesized by polymerization of acetyl and propionyl subunits by classic enzymes as well as iterative and ...
The same protein may act as the subunit of both the pentamers and hexamers or they may be composed of different proteins.[81]. ... When the adaptive immune system of a vertebrate encounters a virus, it produces specific antibodies that bind to the virus and ... Counting these areas and multiplying by the dilution factor allowed him to calculate the number of viruses in the original ... Safety, tolerability and efficacy of peginterferon alpha-2a and ribavirin in chronic hepatitis C in clinical practice: The ...
X-ray crystallographic structure of the 30S ribosomal subunit with bound drug (purple, space-filling model, at center) protein ... Volume 1. Core Clinical Skills. Oxford University Press. 2010. *^ referenced in Treatment of Mitochodrial Disease: Bindu LH, ... PubMed:19031229] See Also Fischel-Ghodsian N. Genetic factors in aminoglycoside toxicity. Ann NY Acad Sci. 1999; 884:99-109. [ ... secondary structure elements such as alpha-helices in bright green, and the RNA phosphodiester backbone shown in orange (and ...
Core Binding Factor alpha Subunits/genetics*. *Embryonal Carcinoma Stem Cells*. *Evolution, Molecular ... Purification and cDNA cloning revealed that PEBP2 has two subunits, DNA-binding alpha (PEBP2alpha) and non-DNA-binding beta ( ... Core-binding factor (CBF), which interacts with a murine retrovirus enhancer, was found to be identical to PEBP2. runt, ... Transcription factors PEA1/AP1, PEA2/PEBP2, and PEA3/ETS were identified as A element-binding proteins. All of them are absent ...
Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously as ... BACKGROUND: Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously ...
What is core-binding factor, runt domain, alpha subunit 1? Meaning of core-binding factor, runt domain, alpha subunit 1 medical ... alpha subunit 1 in the Medical Dictionary? core-binding factor, runt domain, alpha subunit 1 explanation free. ... What does core-binding factor, runt domain, alpha subunit 1 mean? ... Looking for online definition of core-binding factor, runt ... Core-binding factor, runt domain, alpha subunit 1 , definition of core-binding factor, runt domain, alpha subunit 1 by Medical ...
... alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2, (10ug), 10 µg. ... Home » cDNA » Mouse cDNA » Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 ( ... MC219266 Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), ... Properties for Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) ( ...
Core Binding Factor alpha Subunits/genetics. *Core Binding Factor alpha Subunits/metabolism ... Core Binding Factor alpha Subunits. Secondary source ID. *GEO/GSE56015. Grant support. *U01HL100405/HL/NHLBI NIH HHS/United ...
Core Binding Factor Alpha 1 Subunit * Core Binding Factor alpha Subunits * Neoplasm Proteins ... Core Binding Factor Alpha 1 Subunit * Core Binding Factor alpha Subunits * Humans ... RUNX2 is a member of the runt family of transcription factors and its expression is restricted to developing osteoblasts and a ... However analysis of the three-dimensional structure of the DNA binding runt domain of the RUNX proteins and its interaction ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related ... The protein produced from the normal RUNX1 gene is part of a protein complex known as core binding factor (CBF). As part of CBF ... Core binding factor acute myeloid leukemia. A rearrangement (translocation) of genetic material involving the RUNX1T1 gene is ... Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. Int J Hematol. 2011 Aug;94(2): ...
SL3-3 enhancer factor 1 alpha A subunit. *SL3/AKV core-binding factor alpha A subunit ... The RUNX2 protein is a transcription factor, which means it attaches (binds) to specific regions of DNA and helps control the ...
... signalling molecules and transcription factors. Definitive HSCs derived during embryogenesis in the aorta-gonad-mesonephros ... Core Binding Factor Alpha 2 Subunit * Proto-Oncogene Proteins c-myb * Zebrafish Proteins ... Haematopoietic stem cell (HSC) homeostasis is tightly controlled by growth factors, signalling molecules and transcription ... factors. Definitive HSCs derived during embryogenesis in the aorta-gonad-mesonephros region subsequently colonize fetal and ...
... while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of ... The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus ... CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding ... RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5-TGTGGT ...
... core-binding factor alpha subunit 1 (Cbfa1; 7.2-fold), osteocalcin (OC; 430-fold), and BSP (BSP; 5.7-fold) transcripts (Fig. 1 ... B and C) Newly formed vessels, identified by CD31+ endothelial cells, penetrated the outer matrix and reached the inner core in ... an inner hypertrophic core (B, D, and F) rich in GAG, Col II, and Col X, and an outer mineralized rim (B, H, J, and L) with a ... 1997) Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. ...
Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (Predicted). ... PREDICTED: transcription initiation factor TFIID subunit 4-like[Cricetulus griseus].. 11. Mus musculus. 12396. Cbfa2t2; ... PREDICTED: transcription initiation factor TFIID subunit 4B-like[Cricetulus griseus].. 10. Cricetulus griseus. 100769929. ... PREDICTED: transcription initiation factor TFIID subunit 4-likeisoform 1 [Bombus impatiens].. 31. Pteropus vampyrus. ...
0 (BMP2 protein, human); 0 (Bone Morphogenetic Protein 2); 0 (Core Binding Factor Alpha 1 Subunit); 0 (Drugs, Chinese Herbal); ... Subunidade alfa 1 de Fator de Liga o ao Core/gen tica. Seres Humanos. Sistema de Sinaliza o das MAP Quinases/efeitos dos f ... 0 (Enzyme Inhibitors); EC 3.2.1.1 (alpha-Amylases); EC 3.4.21.62 (Subtilisin). ...
Core Binding Factor Alpha Subunit 2 Translocated To 2 (. *. Detection Range: 0.313 ng/ml - 20 ng/ml ... Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target... read more ...
core binding factor Runt domain,alpha subunit 2,(RUNX1) translocated to 2,mainly expressed in brain,with several isoforms due ... Core-binding factor, runt domain, alpha subunit 2; translocated to, 2:. *Hs.153934 ... Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 2 2 3 ... Transcription Factor. Binding Sites. Gene Targets. GH20J033488. Promoter/Enhancer. 2.1. EPDnew Ensembl ENCODE 666.8. 0.0. -23. ...
core binding factor Runt domain,alpha subunit 2,(RUNX1) translocated to 2,mainly expressed in brain,with several isoforms due ... Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 2 2 3 ... Transcription Factor. Binding Sites. Gene Targets. GH20J033400. Promoter/Enhancer. 2.1. EPDnew Ensembl ENCODE CraniofacialAtlas ... Top Transcription factor binding sites by QIAGEN in the CBFA2T2 gene promoter:. *Brachyury ...
... core binding factor plays a key role in several development pathways and in human disease; has been sequenced ... Transcription Factors: 20597*Core Binding Factors: 223*Core Binding Factor alpha Subunits: 18*core binding factor alpha: 16 ... core binding factor alpha. Subscribe to New Research on core binding factor alpha ... 11/07/2000 - "Binding of CBFalpha/AML/PEBP2alpha (core binding factor alpha/acute myelogenous leukemia/polyoma enhancer binding ...
The analysis leads to a better insight of proteins that bind to DNA, regulate DNA, and function in chromatin remodeling. The ... The study summarizes transcriptional regulation factors interacting and cooperating at promoter regions that regulate gene ... Core-binding factor alpha-subunit 1. Osteoblast-specific transcription factor. Cleidocranial dysplasia murine Cbfa1+/- ... Transcription factors bind to regulatory elements upstream of transcription start sites and interact with other factors to ...
AML2SL3/AKV core-binding factor alpha C subunit. *CBFA3. *CBFA3MGC16070. *CBF-alpha-3 ...
AML2SL3/AKV core-binding factor alpha C subunit. *CBFA3. *CBFA3MGC16070. *CBF-alpha-3 ...
CBF-alpha-2; core binding factor alpha 2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit ... SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor ... Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; runt domain, alpha subunit 2; Runt-related transcription factor ... DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding transcription factor binding ...
Core-binding factor subunit alpha-1. FDP:. Fixed dental prostheses. RUNX2:. Runt-related transcription factor 2 ... a transcription factor on chromosome 6p21, have been identified as the cause [3]. This transcription factor is responsible for ... factors such as risk of chipping, esthetics [34], and costs must be weighed. In this case, no ceramic-veneered FDPs were ...
Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (CBFA2T3), transcript variant 2 ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4 ... Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. ... Adenovirus with ORF of interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40 ...
Core Binding Factor Alpha 2 Subunit. *MicroRNAs. *DNA-Binding Proteins. *FISH. *TCF3 ... bHLH transcription factor binding - cell development - chromatin binding - cytoplasm - DNA binding - E-box binding - G1 phase ... transcription factor binding - transcription factor complex - transcription regulatory region DNA binding - transcription, DNA- ... sequence-specific DNA binding - sequence-specific DNA binding transcription factor activity - T cell differentiation in thymus ...
Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Assay Type: Probe Assay Design: Exonic Application: Gene ... Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Assay Type: SYBR® Green Assay Design: Exonic Application ... Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting ...
Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription ... factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Can repress the ... cDNA FLJ33666 fis, clone BRAMY2027752, highly similar to Homo sapiens core-binding factor, runt domain, alpha subunit 2; ... cDNA FLJ33666 fis, clone BRAMY2027752, highly similar to Homo sapiens core-binding factor, runt domain, alpha subunit 2; ...
Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface.. EMBO J. 19 1346-56 2000 ... In higher eukaryotes, the general transcription factor TFIIE consists of two subunits: the large alpha subunit (IPR002853) and ... Transcription initiation factor IIE subunit alpha, N-terminal (IPR002853). Short name: TFIIE_asu ... Structure of the central core domain of TFIIEbeta with a novel double-stranded DNA-binding surface.. EMBO J. 19 1346-56 2000 ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 3. *Omim ID: ... and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi ...
... alpha subunit 2; translocated to, 2) for WB. Anti-MTGR1 pAb (GTX16137) is tested in Human samples. 100% Ab-Assurance. ... core-binding factor, runt domain, alpha subunit 2; translocated to, 2. Background. Myeloid tumor related gene (MTGR1) is found ...
30.core binding factor alpha 2 subunit 9 ƪ6.000%. 31.rna, messenger 9 ƪ6.000% ... 08.promyelocytic leukemia-retinoic acid receptor alpha fusion oncoprotein 18 ƪ12.000% ...
Core-binding factor subunit alpha-1; Short=CBF-alpha-1;AltName: Full=Oncogene AML-3;AltName: Full=Osteoblast-specific ... the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. Virology. 1993 May;194(1):314-31 ... J:19082 Ogawa E, et al., PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila ... J:49076 Xiao ZS, et al., Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor. ...
  • Transcription factors PEA1/AP1, PEA2/PEBP2, and PEA3/ETS were identified as A element-binding proteins. (nih.gov)
  • BACKGROUND: Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously as oncoproteins and tumor suppressors to leukemia and other cancers. (uky.edu)
  • RUNX2 is essential for osteoblastic differentiation and skeletal morphogenesis, and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. (thefreedictionary.com)
  • RUNX2 is a member of the runt family of transcription factors and its expression is restricted to developing osteoblasts and a subset of chondrocytes. (nih.gov)
  • The RUNX2 protein is a transcription factor, which means it attaches (binds) to specific regions of DNA and helps control the activity of particular genes. (medlineplus.gov)
  • Parathyroid Hormone-responsive Smad3-related Factor, Tmem119, Promotes Osteoblast Differentiation and Interacts with the Bone Morphogenetic Protein-Runx2 Pathway. (jax.org)
  • Foxo1 mediates insulin-like growth factor 1 (IGF1)/insulin regulation of osteocalcin expression by antagonizing Runx2 in osteoblasts. (jax.org)
  • In previous studies by our group, we reported that thymosin beta 4 (Tb4) is closely associated with the initiation and development of the tooth germ, and can induce the expression of runt-related transcription factor 2 (RUNX2) during the development of the tooth germ. (nih.gov)
  • Schematic illustration of the putative signaling pathways from thymosin beta 4 (Tb4) to runt-related transcription factor 2 (RUNX2) in the mDE6 cells. (nih.gov)
  • RUNX2 can upregulate the expression of downstream biological effectors, including odontogenesis-related genes and calcification-related factors. (nih.gov)
  • MBS934869 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. (mybiosource.com)
  • Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. (mybiosource.com)
  • Here we show that the endogenous Yes-associated protein (YAP), a mediator of Src/Yes signaling, interacts with the native Runx2 protein, an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. (elsevier.com)
  • Immunofluorescence, immunohistochemistry, and quantitative polymerase chain reaction were performed to evaluate runt-related transcription factor 2 (RUNX2), osteonectin, interleukin (IL)-13, and RUNX2 downstream gene expression. (bvsalud.org)
  • In nonmutant mice, they found that the protein complex composed of Cbf-beta and the Runx2 transcription factor binds to the Wnt10b promoter to drive Wnt10b expression. (eurekalert.org)
  • The Cbf-beta/Runx2 complex also inhibited expression of the enhancer protein C/EBP-alpha that promotes differentiation of adipocytes. (eurekalert.org)
  • Forms the heterodimeric complex core-binding factor (CBF) with CBFB. (uniprot.org)
  • RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. (uniprot.org)
  • Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. (trademetro.net)
  • Mammalian CBFB encodes a transcription factor (CBFbeta) that in combination with CBFalpha2 binds to specific DNA sequences and regulates expression of a number of hematopoietic genes. (zfin.org)
  • We have also analyzed the expression of scl and gata-1 in the same hematopoietic mutants to ascertain the relative order of these transcription factors to cbfb in zebrafish hematopoiesis. (zfin.org)
  • 1982 ). Further study revealed that PEBP2/CBFB was composed of dimers of a DNA-binding subunit (CBF-α) and a non-DNA-binding subunit (CBF-β). (springer.com)
  • The five genes were DIRAS3 (DIRAS family, GTP-binding RAS-like 3), CXCL6 (chemokine (C-X-C motif) ligand 6), SAMD5 (sterile alpha motif domain containing 5), CBFB (core-binding factor, beta subunit), and MEIS2 (meis homeobox 2). (molvis.org)
  • RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. (acris-antibodies.com)
  • Polyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH-conjugated synthetic peptides containing sequences from the central region of the protein. (diagenode.com)
  • CBFb (UniProtKB/Swiss-Prot entry Q13951) represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. (diagenode.com)
  • CBFB enhances DNA binding by RUNX1. (nih.gov)
  • uHTS identification of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a fluorescence resonance energy transfer (FRET) assay. (nih.gov)
  • Confirmation of compounds inhibiting the binding between the RUNX1 Runt domain and CBFb-SMMHC via a time resolved fluorescence resonance energy transfer (TR-FRET) assay. (nih.gov)
  • One of the genes that encodes a CBF alpha subunit is AML1, also called Cbf alpha 2. (curehunter.com)
  • Point mutations introduced into the core site significantly shifted the disease specificity of the Moloney virus from thymic leukemia to erythroid leukemia (N.A. Speck, B. Renjifo, E. Golemis, T.N. Fredrickson, J.W. Hartley, and N. Hopkins, Genes Dev. (asm.org)
  • To identify genes whose expressions in primary human trabecular meshwork (TM) cell cultures are affected by the transcription factor pituitary homeobox 2 (PITX2) and to identify genes that may have roles in glaucoma. (molvis.org)
  • These specialized sigma factors bind the promoters of genes appropriate to the environmental conditions, increasing the transcription of those genes. (wikipedia.org)
  • σ 70 (RpoD) - σ A - the "housekeeping" sigma factor or also called as primary sigma factor , transcribes most genes in growing cells. (wikipedia.org)
  • Every cell has a "housekeeping" sigma factor that keeps essential genes and pathways operating. (wikipedia.org)
  • Runx1/AML1 is a transcription factor implicated in tissue stem cell regulation and belongs to the small Runx family of cancer genes. (elsevier.com)
  • These transcription factors regulate a host of genes specific to haematopoiesis (e.g. (diagenode.com)
  • The protein encoded by this gene is the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family which master-regulates a host of genes specific to hematopoiesis (e.g. (nih.gov)
  • The study summarizes transcriptional regulation factors interacting and cooperating at promoter regions that regulate gene expression. (biomedcentral.com)
  • Transcriptional regulation factors interacting and cooperating at promoter regions determine the fate of cells and their function. (biomedcentral.com)
  • The knowledge evolve transcription factor binding sites on the promoter will allow the creation of the regulation map of each gene and will enable the recognition of other transcription regulation systems that control gene expression. (biomedcentral.com)
  • Initiation of eukaryotic mRNA transcription requires melting of promoter DNA with the help of the general transcription factors TFIIE and TFIIH. (ebi.ac.uk)
  • TFIIE beta has been found to bind to the region where the promoter starts to open to be single-stranded upon transcription initiation by RNA polymerase II. (ebi.ac.uk)
  • A sigma factors binds RNA polymerase through the alpha subunit and then helps the core enzyme detect or a recognize a specific DNA sequence, this is called a promoter. (wikibooks.org)
  • They also help core RNA polymerase locate the consensus promoter sequences near the beginning of a gene. (wikibooks.org)
  • the AML1 (show RUNX1 ELISA Kits )- ETO fusion protein increases the expression of SIRT1 (show SIRT1 ELISA Kits ), possibly by binding to the promoter region of SIRT1 (show SIRT1 ELISA Kits ) to activate its transcription in t(8;21) AML (show RUNX1 ELISA Kits ). (antibodies-online.com)
  • A transcription factor and its associated transcription mediator complex must be attached to a DNA binding site called a promoter region before RNAP can initiate the DNA unwinding at that position. (wikipedia.org)
  • The chromatin immunoprecipitation (ChIP) assay was used to assess the direct binding of HIF1α to the Smad7 promoter. (springernature.com)
  • We also show that Cbfa1 regulates positively the activity of its own promoter, which has the highest affinity Cbfa1-binding sites characterized. (tmc.edu)
  • Identification of IFN regulatory factor-1 binding site in IL-12 p40 gene promoter. (labome.org)
  • A sigma factor is the promoter specificity subunit of eubacterial-type multisubunit RNA polymerases, those whose core subunit composition is often described as alpha(2)-beta-beta-prime. (cathdb.info)
  • Although sigma does not bind DNA on its own, when combined with the core to form the holoenzyme, this binds specifically to promoter sequences, with the sigma factor making sequence specific contacts with the promoter elements. (cathdb.info)
  • Any process involved in the assembly of the RNA polymerase preinitiation complex (PIC) at the core promoter region of a DNA template, resulting in the subsequent synthesis of RNA from that promoter. (cathdb.info)
  • PREDICTED: transcription initiation factor TFIID subunit 4B-like[Cricetulus griseus]. (bioinformatics.org)
  • PREDICTED: transcription initiation factor TFIID subunit 4-likeisoform 1 [Bombus impatiens]. (bioinformatics.org)
  • The first would be the initiation step, during which the RNA polymerase II (RNAPII) binds to the DNA site in order to form a preinitiation complex with other transcriptional factors. (wikibooks.org)
  • A sigma factor ( σ factor ) is a protein needed only for initiation of transcription. (wikipedia.org)
  • [1] It is a bacterial transcription initiation factor that enables specific binding of RNA polymerase to gene promoters . (wikipedia.org)
  • Once initiation of RNA transcription is complete, the sigma factor can leave the complex. (wikipedia.org)
  • Domain structure of a human general transcription initiation factor, TFIIF. (labome.org)
  • The protein produced from the normal RUNX1 gene is part of a protein complex known as core binding factor (CBF). (medlineplus.gov)
  • A common chromosomal translocation in acute myeloid leukemia (AML) involves the AML1 (acute myeloid leukemia 1, also called RUNX1, core binding factor protein (CBF alpha), and PEBP2 alpha B) gene on chromosome 21 and the ETO (eight-twenty one, also called MTG8) gene on chromosome 8. (curehunter.com)
  • runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl, and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche. (zfin.org)
  • Runt-related transcription factor 1 (RUNX1) deficiency attenuates inflammation-induced pro-inflammatory and pro-labour mediators in myometrium. (genetex.com)
  • Web-based predictions of transcription factor binding revealed a binding site for runt-related transcription factor 1 (RUNX1), when the allele at the center of the palindrome (TGGGG(A/G)CCCCA) was A but not when it was G. Furthermore, electromobility shift assay (EMSA) presented evidence in support of allele-specific binding of RUNX1 to 5'HS4. (cdc.gov)
  • Here we show that the mouse transcription factor Runx1, a key regulator of myeloid cell proliferation and differentiation, is expressed in forebrain amoeboid microglia during the first two postnatal weeks. (unboundmedicine.com)
  • The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. (genecards.org)
  • PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene. (jax.org)
  • The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. (diagenode.com)
  • it allosterically enhances DNA binding by alpha subunit as the complex binds to the core site of various enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers and GM-CSF promoters. (nih.gov)
  • Purification and cDNA cloning revealed that PEBP2 has two subunits, DNA-binding alpha (PEBP2alpha) and non-DNA-binding beta (PEBP2beta). (nih.gov)
  • Core-binding factor (CBF), which interacts with a murine retrovirus enhancer, was found to be identical to PEBP2. (nih.gov)
  • Molecular cloning and characterization of PEBP2 beta, the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. (jax.org)
  • Investigating the regulatory mechanism of viral replication led to the identification of the polyomavirus enhancer-binding protein 2 (PEBP2), also known as core-binding factor-β (CBF-β) (Katinka et al. (springer.com)
  • Cloning, mapping and expression of PEBP2 alpha C, a third gene encoding the mammalian Runt domain. (springer.com)
  • The subnuclear organization of nucleic acids and cognate regulatory factors suggests that there are functional interrelationships between nuclear structure and gene expression. (umassmed.edu)
  • Mechanisms that control the spatial distribution of transcription factors within the three-dimensional context of the nucleus may involve the sorting of regulatory information, as well as contribute to the assembly and activity of sites that support gene expression. (umassmed.edu)
  • Molecular, cellular, genetic and biochemical approaches have identified distinct protein segments, termed intranuclear-targeting signals, that are responsible for directing regulatory factors to specific subnuclear sites. (umassmed.edu)
  • Gene rearrangements that remove or alter intranuclear-targeting signals are prevalent in leukemias and have been linked to altered localization of regulatory factors within the nucleus. (umassmed.edu)
  • Assistance at UAB also was provided by the Center for Metabolic Bone Disease, the Small Animal Phenotyping Core, the Metabolism Core and the Neuroscience Molecular Detection Core Laboratory. (eurekalert.org)
  • Here, we show that similarly to its homolog in animal systems, the plant RETINOBLASTOMA-RELATED (RBR) protein regulates the differentiation of meristematic cells at the transition zone by allowing mRNA accumulation of AUXIN RESPONSE FACTOR19 ( ARF19 ), a transcription factor involved in cell differentiation. (plantcell.org)
  • Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. (wikipedia.org)
  • The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). (uniprot.org)
  • Cloning and characterization of subunits of the T-cell receptor and murine leukemia virus enhancer core-binding factor. (asm.org)
  • CBF binds to core sites in murine leukemia virus and T-cell receptor enhancers. (asm.org)
  • Calcium-dependent regulation of tumour necrosis factor-alpha receptor signalling by copine. (springer.com)
  • Alkaline phosphatase (ALP) activity was determined, and reverse transcription polymerase chain reaction was used to measure the expressions of runt-related transcription factor 2 (Runx-2), osteocalcin (OCN), bone sialoproteins (BSP), receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) mRNA. (bvsalud.org)
  • Kikuchi Y, Yasue T, Miyake K, Kimoto M, Takatsu K. CD38 ligation induces tyrosine phosphorylation of Bruton tyrosine kinase and enhanced expression of interleukin 5-receptor alpha chain: synergistic effects with interleukin 5. (labome.org)
  • These data indicate that CD38 ligation increases IL-5 receptor alpha expression and synergizes with IL-5 to enhance Blimp1 expression and IgM synthesis. (labome.org)
  • The human GM-CSF core structure consists of a four α-helix bundle, and GM-CSF activity is controlled by its binding to a two-subunit receptor. (eurekaselect.com)
  • A number of residues located on the B and C helices of GM-CSF are postulated to interact with the alpha chain of the GM-CSF receptor (GM-CSFR). (eurekaselect.com)
  • Several approaches have been successfully utilized to develop peptide mimetics of this site, including peptides from the native sequence, a peptide derived from a recombinant antibody (rAb) light chain which mimicked GM-CSF receptor binding activity, and structurally guided de novo design. (eurekaselect.com)
  • A peptide designed to retain the loop conformation of the CDR I region of the rAb light c hain competed with GM-CSF for both antibody and receptor binding, but the role of specific residues in antibody versus receptor binding differed markedly. (eurekaselect.com)
  • These studies suggest that structural analysis of peptide mimetics can reveal differences in receptor and antibody binding, perhaps including key interactions that impact binding kinetics. (eurekaselect.com)
  • We show that both RBR and the cytokinin-dependent transcription factor ARABIDOPSIS RESPONSE REGULATOR12 are required to activate the transcription of ARF19 , which is involved in promoting cell differentiation and thus root growth. (plantcell.org)
  • In higher eukaryotes, the general transcription factor TFIIE consists of two subunits: the large alpha subunit ( IPR002853 ) and the small beta ( IPR003166 ). (ebi.ac.uk)
  • The approximately 120-residue central core domain of TFIIE beta plays a role in double-stranded DNA binding of TFIIE [ PMID: 10716934 ]. (ebi.ac.uk)
  • CBF beta p22.0, CBF beta p21.5, and CBF beta p17.6 do not by themselves bind the core site. (asm.org)
  • However, CBF beta p22.0 and CBF beta p21.5 form a complex with DNA-binding CBF alpha subunits and as a result decrease the rate of dissociation of the CBF protein-DNA complex. (asm.org)
  • Association of the CBF beta subunits does not extend the phosphate contacts in the binding site. (asm.org)
  • We propose that CBF beta is a non-DNA-binding subunit of CBF and does not contact DNA directly. (asm.org)
  • Role of transforming growth factor beta in human disease. (springer.com)
  • RUNX3 suppresses gastric epithelial cell growth by inducing p21(WAF1/Cip1) expression in cooperation with transforming growth factor {beta}-activated SMAD. (springer.com)
  • The core structure of IHF consists of a partly opened 4-helical bundle that is capped with a beta-sheet. (ebi.ac.uk)
  • RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa , a beta (β) subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. (wikipedia.org)
  • In some cases, a pericentric inversion of chromosome 16 [inv(16)(p13q22)] produces a chimeric transcript consisting of the N terminus of core-binding factor beta in a fusion with the C-terminal portion of the smooth muscle myosin heavy chain 11. (nih.gov)
  • Led by Yi-Ping Li, Ph.D., UAB professor of pathology, and Wei Chen, M.D., UAB associate professor of pathology, they investigated the key role played by Cbf-beta, or core-binding factor subunit beta. (eurekalert.org)
  • a seven-stranded mixed {beta}-sheet flanked on either side by {alpha}-helices. (osti.gov)
  • In addition to this major tertiary structural element, there is a four-stranded antiparallel {beta}-sheet that provides the platform necessary for subunit-subunit assembly. (osti.gov)
  • This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. (wikipedia.org)
  • It is homologous to archaeal transcription factor B and to eukaryotic TFIIB . (wikipedia.org)
  • A redox factor protein, ref1, is involved in negative gene regulation by extracellular calcium. (labome.org)
  • These modifications in the intranuclear targeting of transcription factors might abrogate fidelity of gene expression in tumor cells by influencing the spatial organization and/or assembly of machineries involved in the synthesis and processing of gene transcripts. (umassmed.edu)
  • The gene expression profiles of UT-7/Epo and UT-7/EN cells were compared using HumanHT-12 v4 BeadChip array and by validation with qRT-PCR. (tamuc.edu)
  • 0.01), whereas β-catenin gene expression remained non-significant at 24, 48, and 72 hours relative to un-supplemented UT-7/EN cells. (tamuc.edu)
  • On the contrary, histone acetylation relaxes chromatin condensation and exposes DNA for TF binding, leading to increased gene expression. (wikipedia.org)
  • In biochemical analyses, cbfbeta binds to human CBFalpha2 and enhances its DNA binding. (zfin.org)
  • A single bacteria species can also make several different sigma factors. (wikibooks.org)
  • A sigma (σ) factor binds to the core, forming the holoenzyme. (wikipedia.org)
  • [2] The specific sigma factor used to initiate transcription of a given gene will vary, depending on the gene and on the environmental signals needed to initiate transcription of that gene. (wikipedia.org)
  • Selection of promoters by RNA polymerase is dependent on the sigma factor that associates with it. (wikipedia.org)
  • Every molecule of RNA polymerase holoenzyme contains exactly one sigma factor subunit, which in the model bacterium Escherichia coli is one of those listed below. (wikipedia.org)
  • The number of sigma factors varies between bacterial species. (wikipedia.org)
  • [1] [4] E. coli has seven sigma factors. (wikipedia.org)
  • Sigma factors are distinguished by their characteristic molecular weights. (wikipedia.org)
  • For example, σ 70 is the sigma factor with a molecular weight of 70 kDa . (wikipedia.org)
  • RNA polymerase holoenzyme complex consisting of core RNA polymerase and a sigma factor executes transcription of a DNA template strand. (wikipedia.org)
  • Different sigma factors are utilized under different environmental conditions. (wikipedia.org)
  • [1] In the case of E. coli and other gram-negative rod-shaped bacteria, the "housekeeping" sigma factor is σ 70 . (wikipedia.org)
  • σ 32 (RpoH) - the heat shock sigma factor, it is turned on when the bacteria are exposed to heat. (wikipedia.org)
  • There are also anti-sigma factors that inhibit the function of sigma factors and anti-anti-sigma factors that restore sigma factor function. (wikipedia.org)
  • The sigma subunit is released from the elongating form of the polymerase and is thus free to act catalytically for multiple RNA polymerase core enzymes. (cathdb.info)
  • Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. (genetex.com)
  • Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. (dartmouth.edu)
  • This transcription factor is responsible for the differentiation of precursor cells into osteoblasts and regulates the differentiation of chondrocytes in the growth plate [ 4 ]. (springer.com)
  • Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. (abcam.com)
  • The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. (genetex.com)
  • The protein encoded by this gene belongs to the SECY/SEC61- alpha family. (wikipedia.org)
  • To determine if Cbfa1 plays a role during bone formation we generated transgenic mice overexpressing Cbfa1 DNA-binding domain (DeltaCbfa1) in differentiated osteoblasts only postnatally. (tmc.edu)
  • Integration host factor (IHF) ( IPR005684 , IPR005685 ) is a small heterodimeric protein that binds the minor groove of DNA in a sequence-specific manner and induces a large bend. (ebi.ac.uk)
  • A major determinant of the thymic disease specificity of Moloney virus genetically maps to the conserved viral core motif in the Moloney virus enhancer. (asm.org)
  • Characterization of functional domains in AML/CBF transcription factors has established that there are at least two regulated events that are required for targeting the factors to transcriptionally active nuclear domains: A nuclear localization signal in the amino terminal region controls nuclear import and retention, and a nuclear matrix targeting signal in the carboxyl region controls association with nuclear matrix-linked sites where transcription occurs. (umassmed.edu)
  • Biochemical characterization of copine: a ubiquitous calcium-dependent, phospholipid-binding protein. (springer.com)
  • Characterization of a coiled-coil copine-binding motif. (springer.com)
  • Kageyama R, Sasai Y, Nakanishi S. Molecular characterization of transcription factors that bind to the cAMP responsive region of the substance P precursor gene. (labome.org)
  • [6] Eukaryotic and archaeal RNA polymerases have a similar core structure and work in a similar manner, although they have many extra subunits. (wikipedia.org)
  • When the cell wants to transcribe the DNA strand, the "TATA binding protein" (transcription factor) attaches to the TATA box and subsequently helps in getting the RNA polymerase to attach there and begin synthesizing the RNA. (wikibooks.org)
  • Transcription factor Runx3 regulates interleukin-15-dependent natural killer cell activation. (springer.com)
  • RUNX3 inhibits the expression of vascular endothelial growth factor and reduces the angiogenesis, growth, and metastasis of human gastric cancer. (springer.com)
  • Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. (medlineplus.gov)
  • [2] Eukaryotic and archaeal RNAPs have more subunits than bacterial ones do, and are controlled differently. (wikipedia.org)
  • Depending on the organism, a RNA polymerase can be a protein complex (multi-subunit RNAP) or only consist of one subunit (single-subunit SNAP, ssSNAP), each representing an independent lineage. (wikipedia.org)
  • Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting your real-time PCR results. (bio-rad.com)
  • Key residues involved in CDR I peptide / GM-CSFR binding were identified by truncation and alteration of individual residues, while the structural elements required to antagonize the biological action of GM-CSF were separately tested in binding and inhibitory activity assays of multiple cyclic analogues. (eurekaselect.com)
  • [4] [5] The core RNA polymerase complex forms a "crab claw" or "clamp-jaw" structure with an internal channel running along the full length. (wikipedia.org)