Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.
A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
Myosin type II isoforms found in smooth muscle.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.
The process of bone formation. Histogenesis of bone including ossification.
Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Established cell cultures that have the potential to propagate indefinitely.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Factors that form a preinitiation complex at promoters that are specifically transcribed by RNA POLYMERASE I.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
One of a pair of excretory organs (mesonephroi) which grows caudally to the first pair (PRONEPHROI) during development. Mesonephroi are the permanent kidneys in adult amphibians and fish. In higher vertebrates, proneprhoi and most of mesonephroi degenerate with the appearance of metanephroi. The remaining ducts become WOLFFIAN DUCTS.
Progenitor cells from which all blood cells derive.
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
The gamete-producing glands, OVARY or TESTIS.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
The processes whereby the internal environment of an organism tends to remain balanced and stable.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
A mutation in which a codon is mutated to one directing the incorporation of a different amino acid. This substitution may result in an inactive or unstable product. (From A Dictionary of Genetics, King & Stansfield, 5th ed)
Any detectable and heritable alteration in the lineage of germ cells. Mutations in these cells (i.e., "generative" cells ancestral to the gametes) are transmitted to progeny while those in somatic cells are not.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.

Three distinct domains in TEL-AML1 are required for transcriptional repression of the IL-3 promoter. (1/859)

A cytogenetically cryptic (12;21) translocation is the most common molecular abnormality identified in childhood acute lymphoblastic leukemia (ALL), and it generates a chimeric TEL-AML1 protein. Fusion of the Helix-Loop-Helix (HLH) (also called the pointed) domain of TEL to AML1 has been suggested to convert AML1 from a transcriptional activator to a repressor. To define the structural features of this chimeric protein required for repression, we analysed the transcriptional activity of a series of TEL-AML1 mutants on the AML1-responsive interleukin-3 (IL-3) promoter, a potentially relevant gene target. Our results demonstrate that TEL-AML1 represses basal IL-3 promoter activity in lymphoid cells, and deletion mutant analysis identified three distinct domains of TEL-AML1 that are required for repression; the HLH (pointed) motif contained in the TEL portion of TEL-AML1, and both the runt homology domain (Rhd) and the 74 amino acids downstream of the Rhd that are present in the AML1 portion of the fusion protein. Although AML1B (and a shorter AML1 isoform, AML1A) have transcriptional activating activity on the IL-3 promoter, fusion of the AML1 gene to the TEL gene generates a repressor of IL-3 expression. Consistent with this activity, freshly isolated human ALL cells that contain TEL-AML1 do not express IL-3.  (+info)

Biallelic and heterozygous point mutations in the runt domain of the AML1/PEBP2alphaB gene associated with myeloblastic leukemias. (2/859)

The AML1 gene encoding the DNA-binding alpha-subunit in the Runt domain family of heterodimeric transcription factors has been noted for its frequent involvement in chromosomal translocations associated with leukemia. Using reverse transcriptase-polymerase chain reaction (RT-PCR) combined with nonisotopic RNase cleavage assay (NIRCA), we found point mutations of the AML1 gene in 8 of 160 leukemia patients: silent mutations, heterozygous missense mutations, and biallelic nonsense or frameshift mutations in 2, 4, and 2 cases, respectively. The mutations were all clustered within the Runt domain. Missense mutations identified in 3 patients showed neither DNA binding nor transactivation, although being active in heterodimerization. These defective missense mutants may be relevant to the predisposition or progression of leukemia. On the other hand, the biallelic nonsense mutants encoding truncated AML1 proteins lost almost all functions examined and may play a role in leukemogenesis leading to acute myeloblastic leukemia.  (+info)

Mutual activation of Ets-1 and AML1 DNA binding by direct interaction of their autoinhibitory domains. (3/859)

The transcription factors Ets-1 and AML1 (the alphaBl subunit of PEBP2/CBF) play critical roles in hematopoiesis and leukemogenesis, and cooperate in the transactivation of the T cell receptor (TCR) beta chain enhancer. The DNA binding capacity of both factors is blocked intramolecularly but can be activated by the removal of negative regulatory domains. These include the exon VII domain for Ets-1 and the negative regulatory domain for DNA binding (NRDB) for alphaB1. Here we report that the direct interaction between the two factors leads to a reciprocal stimulation of their DNA binding activity and activation of their transactivation function. Detailed mapping revealed two independent contact points involving the exon VII and NRDB regions as well as the two DNA binding domains. Using deletion variants and dominant interfering mutants, we demonstrate that the interaction between exon VII and NRDB is necessary and sufficient for cooperative DNA binding. The exon VII and NRDB motifs are highly conserved in evolution yet deleted in natural variants, suggesting that the mechanism described is of biological relevance. The mutual activation of DNA binding of Ets and AML1 through the intermolecular interaction of autoinhibitory domains may represent a novel principle for the regulation of transcription factor function.  (+info)

A novel ubiquitin-specific protease, UBP43, cloned from leukemia fusion protein AML1-ETO-expressing mice, functions in hematopoietic cell differentiation. (4/859)

Using PCR-coupled subtractive screening-representational difference analysis, we have cloned a novel gene from AML1-ETO knockin mice. This gene is highly expressed in the yolk sac and fetal liver of the knockin mice. Nucleotide sequence analysis indicates that its cDNA contains an 1,107-bp open reading frame encoding a 368-amino-acid polypeptide. Further protein sequence and protein translation analysis shows that it belongs to a family of ubiquitin-specific proteases (UBP), and its molecular mass is 43 kDa. Therefore, we have named this gene UBP43. Like other ubiquitin proteases, the UBP43 protein has deubiquitinating enzyme activity. Protein ubiquitination has been implicated in many important cellular events. In wild-type adult mice, UBP43 is highly expressed in the thymus and in peritoneal macrophages. Among nine different murine hematopoietic cell lines analyzed, UBP43 expression is detectable only in cell lines related to the monocytic lineage. Furthermore, its expression is regulated during cytokine-induced monocytic cell differentiation. We have investigated its function in the hematopoietic myeloid cell line M1. UBP43 was introduced into M1 cells by retroviral gene transfer, and several high-expressing UBP43 clones were obtained for further study. Morphologic and cell surface marker examination of UBP43/M1 cells reveals that overexpression of UBP43 blocks cytokine-induced terminal differentiation of monocytic cells. These data suggest that UBP43 plays an important role in hematopoiesis by modulating either the ubiquitin-dependent proteolytic pathway or the ubiquitination state of another regulatory factor(s) during myeloid cell differentiation.  (+info)

Regulation of c-fos gene transcription and myeloid cell differentiation by acute myeloid leukemia 1 and acute myeloid leukemia-MTG8, a chimeric leukemogenic derivative of acute myeloid leukemia 1. (5/859)

Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Jurkat T-cells, but not by that of the short form of acute myeloid leukemia 1-MTG8, a chimeric acute myeloid leukemia 1 protein. In 32Dcl3 myeloid cells, stable overexpression of acute myeloid leukemia 1-MTG8 blocked the c-fos gene transcription and cell differentiation, but that of acute myeloid leukemia did not. These data suggest that acute myeloid leukemia 1 and acute myeloid leukemia 1-MTG8 reciprocally regulate the myeloid cell differentiation, possibly by the way of regulating c-fos gene transcription.  (+info)

Solution properties of the free and DNA-bound Runt domain of AML1. (6/859)

The Runt domain is responsible for specific DNA and protein-protein interactions in a family of transcription factors which includes human AML1. Structural data on the Runt domain has not yet become available, possibly due to solubility and stability problems with expressed protein fragments. Here we describe the optimization and characterization of a 140-residue fragment, containing the Runt domain of AML1, which is suitable for structural studies. The fragment of AML1 including amino acids 46-185 [AML1 Dm(46-185)] contains a double cysteine-->serine mutation which does not affect Runt domain structure or DNA-binding affinity. Purified AML1 Dm(46-185) is soluble and optimally stable in a buffer containing 200 mm MgSO4 and 20 mm sodium phosphate at pH 6.0. Nuclear magnetic resonance and circular dichroism spectroscopy indicate that the Runt domain contains beta-sheet, but little or no alpha-helical secondary structure elements. The 45 N-terminal residues of AML1 are unstructured and removal of the N-terminal enhances sequence-specific DNA binding. The NMR spectrum of AML1 Dm(46-185) displays a favorable chemical shift dispersion and resolved NOE connectivities are readily identified, suggesting that a structure determination of this Runt domain fragment is feasible. A titration of 15N-labelled AML1 Dm(46-185) with a 14-bp cognate DNA duplex results in changes in the 15N NMR heteronuclear single quantum coherence spectrum which indicate the formation of a specific complex and structural changes in the Runt domain upon DNA binding.  (+info)

Induction of apoptosis in myeloid leukaemic cells by ribozymes targeted against AML1/MTG8. (7/859)

The translocation (8;21)(q22;q22) is a karyotypic abnormality detected in acute myeloid leukaemia (AML) M2 and results in the formation of the chimeric fusion gene AML1/MTG8. We previously reported that two hammerhead ribozymes against AML1/MTG8 cleave this fusion transcript and also inhibit the proliferation of myeloid leukaemia cell line Kasumi-1 which possesses t(8;21)(q22;q22). In this study, we investigated the mechanisms of inhibition of proliferation in myeloid leukaemic cells with t(8;21)(q22;q22) by ribozymes. These ribozymes specifically inhibited the growth of Kasumi-1 cells, but did not affect the leukaemic cells without t(8;21)(q22;q22). We observed the morphological changes including chromatin condensation, fragmentation and the formation of apoptotic bodies in Kasumi-1 cells incubated with ribozymes for 7 days. In addition, DNA ladder formation was also detected after incubation with ribozymes which suggested the induction of apoptosis in Kasumi-1 cells by the AML1/MTG8 ribozymes. However, the ribozymes did not induce the expression of CD11b and CD14 antigens in Kasumi-1 cells. The above data suggest that these ribozymes therefore inhibit the growth of myeloid leukaemic cells with t(8;21)(q22;q22) by the induction of apoptosis, but not differentiation. We conclude therefore that the ribozymes targeted against AML1/MTG8 may have therapeutic potential for patients with AML carrying t(8;21)(q22;q22) while, in addition, the product of the chimeric gene is responsible for the pathogenesis of myeloid leukaemia.  (+info)

Expression of AML1-d, a short human AML1 isoform, in embryonic stem cells suppresses in vivo tumor growth and differentiation. (8/859)

The human AML1 gene encodes a heterodimeric transcription factor which plays an important role in mammalian hematopoiesis. Several alternatively spliced AML1 mRNA species were identified, some of which encode short protein products that lack the transactivation domain. When transfected into cells these short isoforms dominantly suppress transactivation mediated by the full length AML1 protein. However, their biological function remains obscure. To investigate the role of these short species in cell proliferation and differentiation we generated embryonic stem (ES) cells overexpressing one of the short isoforms, AML1-d, as well as cells expressing the full length isoforms AML1-b and AML2. The in vitro growth rate and differentiation of the transfected ES cells were unchanged. However, overexpression of AML1-d significantly affected the ES cells' ability to form teratocarcinomas in vivo in syngeneic mice, while a similar overexpression of AML1-b and AML2 had no effect on tumor formation. Histological analysis revealed that the AML1-d derived tumors were poorly differentiated and contained numerous apoptotic cells. These data highlight the pleiotropic effects of AML1 gene products and demonstrate for the first time an in vivo growth regulation function for the short isoform AML1-d.  (+info)

TY - JOUR. T1 - Prognostic value of AML 1/ETO fusion transcripts in patients with acute myelogenous leukemia.. AU - Cho, Eun Kyung. AU - Bang, Soo Mee. AU - Ahn, Jeong Yeal. AU - Yoo, Seung Min. AU - Park, Pil Whan. AU - Seo, Yieh Hea. AU - Shin, Dong Bok. AU - Lee, Jae Hoon. PY - 2003/1/1. Y1 - 2003/1/1. N2 - BACKGROUND: The t (8;21) (q22;q22), which produces the fusion gene AML1/ETO, is associated with relatively good prognosis and, in particular, with a good response to cytosine arabinoside. Analysis of t (8;21) positive leukemic blasts has shown characteristic morphological and immunological features. We performed this study to investigate the incidence of AML1/ETO rearrangement in adult acute myelogenous leukemia (AML), especially in M2 subtype, to make a comparison of clinical, morphological and immunophenotypic characteristics between AML1/ETO rearrangement positive and negative group in patients with AML and to analyze the correlation with other biological parameters. METHODS: From May ...
The t(8;21) acute myeloid leukemia (AML)-associated oncoprotein AML1-ETO disrupts normal hematopoietic differentiation. Here, we have investigated its effects on the transcriptome and epigenome in t(8,21) patient cells. AML1-ETO binding was found at promoter regions of active genes with high levels of histone acetylation but also at distal elements characterized by low acetylation levels and binding of the hematopoietic transcription factors LYL1 and LMO2. In contrast, ERG, FLI1, TAL1, and RUNX1 bind at all AML1-ETO-occupied regulatory regions, including those of the AML1-ETO gene itself, suggesting their involvement in regulating AML1-ETO expression levels. While expression of AML1-ETO in myeloid differentiated induced pluripotent stem cells (iPSCs) induces leukemic characteristics, overexpression increases cell death. We find that expression of wild-type transcription factors RUNX1 and ERG in AML is required to prevent this oncogene overexpression. Together our results show that the interplay ...
KIT gene mutations occur in approximately 25% of cases of acute myeloid leukemia (AML) with t(8;21) or inv(16), also referred to as core-binding factor AML. These mutations are predominately small length affecting mutations in KIT exon 8 and substitution mutations in KIT exon 17. KIT mutations in core-binding factor AML may be an adverse prognostic factor in this otherwise favorable-risk disease. This DNA sequencing test detects mutations in exons 8 and 17 of the KIT gene in blood and bone marrow specimens. This test will detect mutations in specimens when at least 30% of the nucleated cells carry the mutation.. ...
2018 The Author(s) Oncogenic transcription factors such as the leukemic fusion protein RUNX1/ETO, which drives t(8;21) acute myeloid leukemia (AML), constitute cancer-specific but highly challenging therapeutic targets. We used epigenomic profiling data for an RNAi screen to interrogate the transcriptional network maintaining t(8;21) AML. This strategy identified Cyclin D2 (CCND2) as a crucial transmitter of RUNX1/ETO-driven leukemic propagation. RUNX1/ETO cooperates with AP-1 to drive CCND2 expression. Knockdown or pharmacological inhibition of CCND2 by an approved drug significantly impairs leukemic expansion of patient-derived AML cells and engraftment in immunodeficient murine hosts. Our data demonstrate that RUNX1/ETO maintains leukemia by promoting cell cycle progression and identifies G1 CCND-CDK complexes as promising therapeutic targets for treatment of RUNX1/ETO-driven AML. Using in vitro and in vivo screens to identify essential RUNX1/ETO transcriptional targets in AML, Martinez-Soria ...
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Loss of RUNX1/ETO Triggers C/EBPα-Driven Reorganization of the Leukemic Transcriptional Network(A) RUNX1/ETO and CEBPA mRNA expression levels in Kasumi-1 cells
CATAGAGCCA GCGGGCGCGG GCGGGACGGG CGCCCCGCGG CCGGACCCAG CCAGGGCACC ACGCTGCCCG GCCCTGCGCC GCCAGGCACT TCTTTCCGGG ^1 ^11 ^21 ^31 ^41 ^51 ^61 ^71 ^81 ^91 GCTCCTAGGG ACGCCAGAAG GAAGTCAACC TCTGCTGCTT CTCCTTGGCC TGCGTTGGAC CTTCCTTTTT TTGTTGTTTT TTTTTGTTTT TCCCCTTTCT ^101 ^111 ^121 ^131 ^141 ^151 ^161 ^171 ^181 ^191 TCCTTTTGAA TTAACTGGCT TCTTGGCTGG ATGTTTTCAA CTTCTTTCCT GGCTGCGAAC TTTTCCCCAA TTGTTTTCCT TTTACAACAG GGGGAGAAAG ^201 ^211 ^221 ^231 ^241 ^251 ^261 ^271 ^281 ^291 TGCTCTGTGG TCCGAGGCGA GCCGTGAAGT TGCGTGTGCG TGGCAGTGTG CGTGGCAGGA TGTGCGTGCG TGTGTAACCC GAGCCGCCCG ATCTGTTTCG ^301 ^311 ^321 ^331 ^341 ^351 ^361 ^371 ^381 ^391 ATCTGCGCCG CGGAGCCCTC CCTCAAGGCC CGCTCCACCT GCTGCGGTTA CGCGGCGCTC GTGGGTGTTC GTGCCTCGGA GCAGCTAACC GGCGGGTGCT ^401 ^411 ^421 ^431 ^441 ^451 ^461 ^471 ^481 ^491 GGGCGACGGT GGAGGAGTAT CGTCTCGCTG CTGCCCGAGT CAGGGCTGAG TCACCCAGCT GATGTAGACA GTGGCTGCCT TCCGAAGAGT GCGTGTTTGC ^501 ^511 ^521 ^531 ^541 ^551 ^561 ^571 ^581 ^591 ATGTGTGTGA CTCTGCGGCT GCTCAACTCC CAACAAACCA GAGGACCAGC ...
Chromosome changes in the bone marrow (BM) of patients with persistent cytopenia are often considered diagnostic for a myelodysplastic syndrome (MDS). Comprehensive cytogenetic evaluations may give evidence of the real pathogenetic role of these changes in cases with cytopenia without morphological signs of MDS. Chromosome anomalies were found in the BM of three patients, without any morphological evidence of MDS: 1) an acquired complex rearrangement of chromosome 21 in a boy with severe aplastic anaemia (SAA); the rearrangement caused the loss of exons 2-8 of the RUNX1 gene with subsequent hypoexpression. 2) a constitutional complex rearrangement of chromosome 21 in a girl with congenital thrombocytopenia; the rearrangement led to RUNX1 disruption and hypoexpression. 3) an acquired paracentric inversion of chromosome 1, in which two regions at the breakpoints were shown to be lost, in a boy with aplastic anaemia; the MPL gene, localized in chromosome 1 short arms was not mutated neither disrupted, but
The TEL-AML1 fusion not only characterizes the most frequent genetic rearrangement in initial childhood ALL (20-25%) but its presence has also been associated with a favorable prognosis (9, 10, 11 , 28 , 32) . In clinical studies on initial ALL, probability of event-free survival (EFS) at 4 years was as high as 90-100% for TEL-AML1+ patients (9 , 10 , 32) . These results certainly do not represent final outcome considering that, regardless of the different prevalence of TEL-AML1 positivity at relapse of BCP-ALL (range, 3- 28%), TEL-AML1+ leukemia is biologically characterized by a long duration of first CR and that the majority of relapses (80%) occur off-therapy (median, 46 months; range, 13-125 months; Refs. 18, 19, 20, 21, 22, 23 , 33 ). The prevalence of TEL-AML1 positivity in our ongoing prospective study on first relapse of BCP-ALL is ∼17% (31 of 178 children; 33 ).. Obviously, the predictive value of TEL-AML1 positivity alone is insufficient to stratify patients to appropriate treatment ...
Further we asked if VLA-4 and VLA-5 integrin upregulation is maintained by RUNX1/ETO in the transformed human leukemia cell line Kasumi-1, derived from a t(8;21)+ AML patient. Kasumi-1 cells, which express RUNX1/ETO and to a lesser extent RUNX1/ETOtr,4 bear high levels of VLA-4 whereas the integrin αL subunit is absent in these cells. We specifically down-regulated RUNX1/ETO via lentivirally delivered shRNA targeting the RUNX1/ETO breakpoint sequences (shRE), which are present in both full length and truncated forms (Online Supplementary Figure S3). At Day 4 after transduction with vectors co-expressing shRE and eGFP, α4, α5 and β1 expression levels were significantly reduced as assessed using flow cytometry, while CXCR4 levels remained unaltered (Figure 1I). Similar results were obtained with NHR2 competitive peptides (N89) (Figure 1J), which also interfere with both RUNX1/ETO forms by disrupting RUNX1/ETO tetramer formation.6 These results suggest that integrin subunit expression remains ...
MGA is an incompletely studied gene with a high mutation frequency in MLL-PTD AML (9%) and in core bind factor AML (8%). This gene encodes a MAX-interacting protein and is believed to act as a transcription factor that suppresses MYC binding to its target. By in silico analysis, we found that MGA is expressed in normal myeloid hematopoietic cells and AML, and the expression level is comparable with TET2 or DNMT3A. Further data mining of TCGA revealed a high frequency of inactivating mutations of the MGA gene in a variety of cancers such as various adenocarcinomas. To interrogate functionally its role in leukemogenesis, lentiviral constructs containing either shRNA or CRISPR-sgRNA targeted to different regions of the MGA gene were generated. MGA expressing AML cell line EOL-1 was silenced by shRNA or CRISPER system. Silencing was confirmed by western blot (shRNA) and Sanger Sequencing (sgRNA). An increase of methylcellulose colony number (~30%) was observed in MGA silenced cell lines. Control ...
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The mechanism underlying the lineage decision made by CD4(+)CD8(+) double-positive (DP) thymocytes that give rise to two T lymphocyte subset with distinct functionalities, that is, helper and cytotoxic T cells, remains a major issue in immunology. The lineage decision process involves several phases and terminates when cells loose their developmental plasticity to become the alternate lineage. A detailed picture of the transcription factor network governing helper versus cytotoxic-lineage decision has recently emerged. Studies published only past year provided new insights into how the expression of ThPOK, a central transcription factor for helper T cell development, is regulated. It has now become evident that an antagonistic interplay between ThPOK and Runx transcription factor complexes plays an essential role in thwarting an alternate fate during the commitment process.
Scientists from the Cancer Science Institute of Singapore (CSI Singapore) at the National University of Singapore (NUS) have discovered that modifications to a protein called RUNX3 may promote cancer progression. The results of the study were published in the prestigious journal Proceedings of the National Academy of Sciences (PNAS) in June 2016. The research team, led by Professor Yoshiaki Ito, Senior Principal Investigator at CSI Singapore, found that a modification called phosphorylation made to RUNX3 promotes cancer progression by allowing cell division. Uncontrolled cell division in the body is a process by which tumours form and hence is a hallmark of cancer. RUNX3 is a tumour suppressor gene that prevents the formation of tumours by binding to DNA. The phosphorylation, or the addition of a phosphate group to a molecule, is carried out by an enzyme called Aurora Kinase, which has been observed to be present in unusually high levels in some cancers. Phosphorylation prevents the binding of ...
Study hypothesis: Treatment with dasatinib 100 mg QD is safe and efficacious when given to patients with Ph+ ALL in the post SCT setting.
The inherited platelet disorders are an uncommon cause of symptomatic bleeding. They may be difficult to diagnose (and are likely to be under-diagnosed) and pose problems in management. This review discusses the inherited platelet disorders summarising the current state of the art with respect to in …
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The RUNX1/AML1 gene is the most frequent target for chromosomal translocation in leukemia. In addition, recent studies have demonstrated point mutations in the RUNX1 gene as another mode of genetic alteration in development of leukemia. Monoallelic germline mutations in RUNX1 result in familial plat …
RUNX1 antibody (runt-related transcription factor 1) for WB. Anti-RUNX1 pAb (GTX11903) is tested in Human, Mouse samples. 100% Ab-Assurance.
RUNX1 antibody (runt-related transcription factor 1) for ICC/IF, WB. Anti-RUNX1 pAb (GTX129100) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
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The Mammalian Phenotype (MP) Ontology is a community effort to provide standard terms for annotating phenotypic data. You can use this browser to view terms, definitions, and term relationships in a hierarchical display. Links to summary annotated phenotype data at MGI are provided in Term Detail reports.
AML-2 (Acute myeloid leukemia 2 or Runx3) antibody against the runt transcription factor 3 for use in supershift (EMSA) and Western blotting.
PHF2라는 단백질이 뼈를 만드는 세포(조골세포)를 활성화시킨다는 사실을 처음으로 규명했다. 조골세포는 Runx2라는 단백질에 의해 분화가 조절된다. 반면, SUV39HI1라는 효소는 Runx2에 메틸기(CH3)를 붙임으로써 Runx2가 기능을 하지 못하게 하는 장식으로 분화를 방해한다. 성장이 끝난 성인들이 더 이상 키가 크지 않는 것도 SUV39HI1 효소 때문이다. 이에 착안해 Runx2에 붙어 있는 메틸기를 제거하는 방안을 연구한 결과, PHF2 단백질이 조골세포 분화를 유도함으로써, 소아의 뼈 발달 과정이나 골절 후 뼈가 새로 형성되는 과정에 작용한다는 것을 증명했다.. PHF2 단백질은 Runx2에 붙어 있는 메틸기를 제거했으며, 이후 본연의 기능을 회복한 Runx2는 조골세포의 분화를 촉진하여 다시 뼈를 만들기 시작했다. 실제 유전자 조작으로 PHF2 단백질이 과발현된 쥐를 만들어 ...
Current Research and Scholarly InterestsInherited mutations in the RUNX1 gene cause a platelet disorder and increased risk of blood cancers. However, it is still unclear what actually causes progression to cancer in these patients. Using genetic editing, I am investigating how RUNX1 mutations contribute to disease. ...
Accountants have been warned to expect an increase in AML compliance work in the wake of a new report criticising bodies for a widespread lack of ML
Answers to FAQs from patients, which will answer any questions that they have. Dr. Stavros Alevrogiannis - MD-Msc-PhD, Tel. Contact: 2107786868.
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Runt-related transcription factor 1 (RUNX1) is generally considered to function as a tumor suppressor in the development of leukemia, but a growing body of evidence suggests that it has pro-oncogenic properties in acute myeloid leukemia (AML). Here we have demonstrated that the antileukemic effect mediated by RUNX1 depletion is highly dependent on a functional p53-mediated cell death pathway. Increased expression of other RUNX family members, including RUNX2 and RUNX3, compensated for the antitumor effect elicited by RUNX1 silencing, and simultaneous attenuation of all RUNX family members as a cluster led to a much stronger antitumor effect relative to suppression of individual RUNX members. Switching off the RUNX cluster using alkylating agent-conjugated pyrrole-imidazole (PI) polyamides, which were designed to specifically bind to consensus RUNX-binding sequences, was highly effective against AML cells and against several poor-prognosis solid tumors in a xenograft mouse model of AML without ...
TY - JOUR. T1 - Loss of runt-related transcription factor 3 induces gemcitabine resistance in pancreatic cancer. AU - Horiguchi, Shigeru. AU - Shiraha, Hidenori. AU - Nagahara, Teruya. AU - Kataoka, Jyunnro. AU - Iwamuro, Masaya. AU - Matsubara, Minoru. AU - Nishina, Shinichi. AU - Kato, Hironari. AU - Takaki, Akinobu. AU - Nouso, Kazuhiro. AU - Tanaka, Takehiro. AU - Ichimura, Koichi. AU - Yagi, Takahito. AU - Yamamoto, Kazuhide. PY - 2013/8. Y1 - 2013/8. N2 - Background & Aim: Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene that is expressed in gastric and other cancers including pancreatic cancer. However, the precise function of RUNX3 in pancreatic cancer has not been fully elucidated. In this study, we aimed to determine the effect of decreased RUNX3 expression in pancreatic cancer. Methods: This study included 36 patients with primary pancreatic cancer, who had undergone pancreaticoduodenectomy. All patients were treated with 1000mg/m2 gemcitabine after the surgery. ...
Runt-related transcription factor 1 (RUNX1) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters and can accelerate apoptosis in various tumors. However, the regulatory mechanisms underlying RUNX1 expression in neuroblastoma (NB), a highly malignant tumor in childhood, remain largely unclear. In this study, we aimed to assess the role of RUNX1 in NB and to reveal the underlying mechanisms that may contribute to finding a potential therapeutics strategy against NB. Growth, invasion, metastasis and angiogenesis were assessed using Cell Counting Kit-8 (CCK-8) immunocytochemistry, and studies involving soft agar, cell invasion, tube formation and whole animals. The levels of expression were measured using real-time quantitative PCR for RNA, Western blot and immunostaining analyses for proteins. Luciferase reporter and chromatin immunoprecipitation assays indicated that RUNX1 directly binds within the BIRC5, CSF2RB and NFKBIA promoter regions to facilitate
Hematopoietic stem cells (HSCs) are self-renewing multipotent stem cells that generate mature blood lineages throughout life. They, together with hematopoietic progenitor cells (collectively known as HSPCs), emerge from hemogenic endothelium in the floor of the embryonic dorsal aorta by an endothelial-to-hematopoietic transition (EHT). Here we demonstrate that transforming growth factor β (TGFβ) is required for HSPC specification and that it regulates the expression of the Notch ligand Jagged1a in endothelial cells prior to EHT, in a striking parallel with the epithelial-to-mesenchymal transition (EMT). The requirement for TGFβ is two fold and sequential: autocrine via Tgfβ1a and Tgfβ1b produced in the endothelial cells themselves, followed by a paracrine input of Tgfβ3 from the notochord, suggesting that the former programs the hemogenic endothelium and the latter drives EHT. Our findings have important implications for the generation of HSPCs from pluripotent cells in vitro.
BACKGROUND: Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously as oncoproteins and tumor suppressors to leukemia and other cancers. To discover fundamental Runx functions in the cell biology of animal development, we have employed morpholino antisense-mediated knockdown of the sea urchin Runx protein SpRunt-1. Previously we showed that embryos depleted of SpRunt-1 arrest development at early gastrula stage and underexpress the conventional protein kinase C SpPKC1. RESULTS: We report here that SpRunt-1 deficiency leads to ectopic cell proliferation and extensive apoptosis. Suppression of the apoptosis by pharmacological inhibition of caspase-3 prevents the ectopic proliferation and rescues gastrulation, indicating that many of the overt defects obtained by knockdown of SpRunt-1 are secondary to the apoptosis. Inhibition or knockdown of SpPKC1 also causes apoptosis, while cell survival is rescued in SpRunt-1 morphant embryos coinjected with
The Runt related transcription factors (RUNX) are recognized as key players in suppressing or promoting tumor growth. RUNX3, a member of this family, is known as a tumor suppressor in many types of cancers, although such a paradigm was challenged by some researchers. The TGF-β pathway governs major upstream signals to activate RUNX3. RUNX3 protein consists of several regions and domains. The Runt domain is a conserved DNA binding domain and is considered as the main part of RUNX proteins since. Herein, we compared the effects of Runt domains and full-Runx3 in cell viability by designing two constructs of Runx3, including N-terminal region and Runt domain. We investigated the effect of full-Runx3, N-t, and RD on growth inhibition in AGS, MCF-7, A549, and HEK293 cell lines which are different in TGF-β sensitivity, in the absence and presence of TGF-β. The full length RUNX3 did not notably inhibit growth of these cell lines while, the N-t and RD truncates showed different trends in these cell lines.
TY - JOUR. T1 - Fusion AML1 transcript in a radiation-associated leukemia results in a truncated inhibitory AML1 protein. AU - Hromas, Robert. AU - Busse, Tracey. AU - Carroll, Audra. AU - Mack, David. AU - Shopnick, Rinah. AU - Zhang, Dong Er. AU - Nakshatri, Harikrishna. AU - Richkind, Kathleen. PY - 2001/4/1. Y1 - 2001/4/1. N2 - AML1 is a transcription factor that is essential for normal hematopoietic development. It is the most frequent target for translocations in acute leukemia. Recently, fluorescence in situ hybridization was used to identify a novel syndrome of radiation-associated secondary acute myelogenous leukemia that had AML1 translocations. Using polymerase chain reaction, the AML1 fusion transcript was isolated from the patient who had a t(19;21) radiation-associated leukemia. The AML1 gene is fused out of frame to chromosome 19 sequences, resulting in a truncated AML protein bearing the DNA binding domain but not the transcriptional activation domain. This fusion AML1 protein ...
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The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
Cancer researchers at the University of Cincinnati College of Medicine have found an obesity-associated proteins role in leukemia development and drug response which could lead to more effective therapies for the illness.
This resource is intended for patients with acute myeloid leukemia (AML). You will find expert advice about AML to help you discuss key issues with your healthcare provider and make important decisions related to management and treatment. Easy-to-understand animations with audio narration, expert video explanations, illustrated slide shows, and educational downloads are available to you.
This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must be interpreted in the context of a patients clinical data using reasonable medical judgment. This website should not be used as a substitute for the advice of a licensed physician ...
The algorithm predicts the chance of the achievement of a complete remission and the risk for an early death for elderly patients (60 years and older) with newly diagnosed AML.
Looking for online definition of core-binding factor, runt domain, alpha subunit 3 in the Medical Dictionary? core-binding factor, runt domain, alpha subunit 3 explanation free. What is core-binding factor, runt domain, alpha subunit 3? Meaning of core-binding factor, runt domain, alpha subunit 3 medical term. What does core-binding factor, runt domain, alpha subunit 3 mean?
The Caenorhabditis elegans run gene encodes a Runt domain factor. Runx1, Runx2, and Runx3 are the three known mammalian homologs of run. Runx1, which plays an essential role in hematopoiesis, has been identified at the breakpoint of chromosome translocations that are responsible for human leukemia. Runx2 plays an essential role in osteogenesis, and inactivation of one allele of Runx2 is responsible for the human disease cleidocranial dysplasia. To understand the role of run in C. elegans, we used transgenic run::GFP reporter constructs and a double-stranded RNA-mediated interference method. The expression of run was detected as early as the bean stage exclusively in the nuclei of seam hypodermal cells and lasted until the L3 stage. At the larval stage, expression of run was additionally detected in intestinal cells. The regulatory elements responsible for the postembryonic hypodermal seam cells and intestinal cells were separately located within a 7.2-kb-long intron region. This is the first ...
core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. Few prognostic markers have been identified. In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. Overall, 10-year overall survival (OS), disease-free survival (DFS), and event-free survival were 63.9%, 54.8%, and 49.9%, respectively; patients with the t(8;21) and inv(16) chromosomal rearrangements exhibited significant differences at diagnosis. Despite similarly high complete remission (CR) rate, patients with inv(16) experienced superior DFS and a high chance of achieving a second CR, often leading to prolonged OS also after relapse. We found that a complex karyotype (ie, ≥4 cytogenetic anomalies) affected survival; the KIT D816 mutation predicted worse prognosis only in patients with the t(8;21) rearrangement, whereas FLT3 mutations had no ...
The nuclear matrix protein, NMP-2, was originally identified as an osteoblast-specific DNA-binding complex localized exclusively to the nuclear matrix. NMP-2 was shown to recognize two binding sites, site A (nt-605 to -599) and site B (nt -441 to -435), in the rat bone-specific osteocalcin gene promoter. This study shows that the NMP-2 binding sites A and B as well as a third NMP-2 binding site (nt -135 to -130) constitute a consensus sequence, ATGCTGGT, and represent an AML-1 recognition motif. AML-1 is a member of the AML transcription factor family which is associated with acute myelogenous leukemia and binds to the sequence TGCTGGT via its DNA-binding runt domain. Electrophoretic mobility shift assays reveal that a component of NMP-2 is a member of the AML/PEBP2/runt domain transcription factor family based on cross-competition with AML-1 consensus oligonucleotide. Limited immunoreactivity of NMP-2 with a polyclonal N-terminal AML-1 antibody and inability of the AML-1 partner protein CBF-beta to
Cell proliferation. To assess cell proliferation, 1 × 105 cells of the indicated AML-derived cells were seeded in 6-well plates. For the tetracycline-inducible gene or shRNA expression, doxycycline was added to the culture at a final concentration of 3 μM. Trypan blue dye exclusion assays were performed every other day.. RT-qPCR. Total RNA was isolated with an RNeasy Mini Kit (Qiagen) and reverse transcribed with a ReverTra Ace kit (TOYOBO) to generate cDNA. RT-qPCR was carried out with a 7500 Real-Time PCR System (Applied Biosystems) according to the manufacturers instructions. The results were normalized to GAPDH levels. Relative expression levels were calculated using the 2-ΔΔCt method. Primers used for RT-qPCR are listed in Supplemental Table 3.. ChIP-qPCR. ChIP was performed using a SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturers instructions. In brief, cells were cross-linked in 1% formaldehyde in PBS for 10 minutes at room ...
Estrogen receptor α (ER α) and androgen receptor (AR) are master transcription factors in the breast and prostate, respectively. They are commonly known in development of sexual characteristics. However, both ERα and AR have been known to be involved in breast cancer (BCa) and prostate cancer (PCa) progression, respectively. The Runx family of transcription factors plays a role in hematopoiesis (Runx1), skeletogenesis (Runx2) and neurogenesis (Runx3). In addition, Runx proteins inhibit cell cycle progression, and have been assigned tumor suppressor roles in various contexts. Because both BCa and PCa cells metastasize to bone at high frequency, investigators have interrogated the possibility that they share characteristics with osteoblasts. Indeed, BCa and PCa cells were found to have osteomimetic properties, including expression of Runx2 and Runx2-target genes otherwise expressed by osteoblasts. Provoked by the reported physical interaction between AR and Runx2, we initiated a study to test ...
Zhen F, Lan Y, Yan B, Zhang W, Wen Z. Hemogenic endothelium specification and hematopoietic stem cell maintenance employ distinct Scl isoforms. Development. 20
In order to ascertain the effectiveness of nutrient rich diet and dietary counseling on the health status of pediatric acute lymphoblastic leukemia patients, this experimental study was conducted at the Institute of Radiology and Nuclear Medicine, Peshawar. A sample of 30 leukemia patients were divided into experimental and control groups based on written consents. Data regarding demographic characteristics, anthropometric measurements, and retrospective food intakes were recorded on self-constructed questionnaire. Patients in the experimental group received dietary guidelines for nutrient rich diet. Anthropometry, dietary evaluation, and blood nutrients namely serum ferritin, albumin, globulin, total protein and creatinine at 30, 60 and 90 days intervals were assessed. The data showed low height for age and low weight or height at diagnosis indicating malnourishment and wasting among all the patients. After nutritional intervention mean weights of patients in the experimental group increased ...
ASSESSMENT OF OBESITY AND HEPATIC LATE ADVERSE EFFECTS IN THE EGYPTIAN SURVIVORS OF PEDIATRIC ACUTE LYMPHOBLASTIC LEUKEMIA: SINGLE CENTER STUDY
ASSESSMENT OF OBESITY AND HEPATIC LATE ADVERSE EFFECTS IN THE EGYPTIAN SURVIVORS OF PEDIATRIC ACUTE LYMPHOBLASTIC LEUKEMIA: SINGLE CENTER STUDY
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
This study is examining the appropriate dose and side effects of dasatinib, when it is given with the standard of care chemotherapy for children and adolescents
van der Deen M, Taipaleenmaki H, Zhang Y, Teplyuk NM, Gupta A, Cinghu S, Shogren K, Maran A, Yaszemski MJ, Ling L, Cool SM, Leong DT, Dierkes C, Zustin J, Salto-Tellez M, Ito Y, Bae SC, Zielenska M, Squire JA, Lian JB, Stein JL, Zambetti GP, Jones SN, Galindo M, Hesse E, Stein GS, van Wijnen AJ. MicroRNA-34c inversely couples the biological functions of the runt-related transcription factor RUNX2 and the tumor suppressor p53 in osteosarcoma. J Biol Chem. 2013 Jul 19; 288(29):21307-19. Epub 2013 May 29 ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
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Among patients with good prognosis core binding factor AML, there is an overall survival rate of only 44%. To understand the genetic factors contributing to poor outcomes within this subgroup, Dr Chew is analysing bone marrow samples collected from 18 patients before and during treatment.. According to Dr Chew, multiple genetic abnormalities acquired during therapy are probably responsible for good prognosis core binding AML developing resistance to chemotherapy.. To help us predict who will respond poorly to therapy, were identifying the genetic mutations occurring in patients who relapse, he said. This information will allow us to tailor patient treatment accordingly. Currently a stem cell transplant is considered the definitive treatment and our findings will help clinicians decide if their patients AML will develop resistance and if a stem cell transplant is recommended.. Dr Chew is testing the usefulness of the genetic variations he identifies through an international ...
Days that platelet count firstly rebound to 75×10^9/L, 100×10^9/L, respectively, from the first time below 75×10^9/L at the chemotherapy cycle and the last chemotherapy ...
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Dagmara Beines daughter Zuza was given an 8% - 15% chance of surviving when she was diagnosed with a particularly aggressive form of AML
Pill with imprint APO AML 10 is White, Round and has been identified as Amlodipine besylate 10 mg. It is supplied by Apotex Corp..
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Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. It was previously believed that the RNA ... Sigma factors in E. coli:. *σ70(RpoD) - σA - the "housekeeping" sigma factor or also called as primary sigma factor, ... Different sigma factors are utilized under different environmental conditions. These specialized sigma factors bind the ...
These core binding factors, or nuclear factors (NF-Y), are composed of three subunits - NF-YA, NF-YB, and NF-YC. Whereas in ... The first domain (A1) contains 20 amino acids that forms an alpha helix that appears significant in its interactions with NF-YB ... It is essential to the transcription that these core binding factors (also referred to as nuclear factor Y or NF-Y) are able to ... the core binding factor (CBF)-DNA complex retains a high degree of conservation within the CCAAT binding motif, as well as the ...
Chen Y; Le Cahérec F; Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. GRCh38: ... Knight BC; High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ...
DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... "New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ...
The core enzyme of RNA polymerase has five subunits (protein subunits) (~400 kDa). Because of the RNA polymerase association ... with sigma factor, the complete RNA polymerase therefore has 6 subunits: the sigma subunit-in addition to the two alpha (α), ... binds to TATA box binding protein (TBP) and recognizes TATA boxTBP associated factors (TAFs) and add promoter selectivity TFIIE ... The RNA polymerase core associates with the sigma factor to form RNA polymerase holoenzyme. Sigma factor reduces the affinity ...
... subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, ... RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa, a beta (β) ... The core enzyme has five subunits (~400 kDa):[23] *β': The β' subunit is the largest subunit, and is encoded by the rpoC gene.[ ... In order to bind promoters, RNAP core associates with the transcription initiation factor sigma (σ) to form RNA polymerase ...
Törö I, Thore S, Mayer C, Basquin J, Séraphin B, Suck D (May 2001). "RNA binding in an Sm core domain: X-ray structure and ... RNA oligonucleotides generally bind inside the hole (lumen) of the LSm torus, one nucleotide per LSm subunit, but additional ... The short (two to four turns) N-terminal alpha helix occurs at one edge of the beta sandwich. This alpha helix and the beta ... the Sm-like protein HF-I encoded by the gene hfq was described in 1968 as an essential host factor for RNA bacteriophage Qβ ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
... sandwiched between the Walker A and B motifs of one subunit and the LSGGQ motif of the other subunit. The maltose binding ... heme-binding protein, and alkaline protease), heme, hydrolytic enzymes, S-layer proteins, competence factors, toxins, ... In addition, a gap in the protein is accessible directly from the hydrophobic core of the inner leaflet of the membrane bilayer ... The T domains are each built of typically 10 membrane spanning alpha helices, through which the transported substance can cross ...
... factor implicated in the tumorigenesis and progression of breast cancer via its binding to the estrogen receptor alpha (ERα). ... Further, HATs have diverse role as transcription factors beside having histone acetylase activity, e.g., HAT subunit, hADA3 may ... The transcriptional regulation of the genome is controlled primarily at the preinitiation stage by binding of the core ... Epigenetics Histone Nucleosomes Chromatin Histone acetyltransferase Transcription factors CAF-1 (Chromatin assembly factor-1 ...
These core histones are rich in lysine and arginine residues. The carboxyl (C) terminal end of these histones contribute to ... It results in good optimization and is used in vivo to reveal DNA-protein binding occurring in cells. ChIP-Seq can be used to ... H3K9ac indicates acetylation of lysine 9 on histone H3 protein subunit: The genomic DNA of eukaryotic cells is wrapped around ... Maruta H, Greer K, Rosenbaum JL (1986). "The acetylation of alpha-tubulin and its relationship to the assembly and disassembly ...
Zipper Coiled-Coil Domains of the cGMP-Dependent Protein Kinase I alpha and its Interaction with the Myosin Binding Subunit of ... Leucine zipper coiled-coil helices & DNA-binding helices: transcription factor Max (PDB file 1HLO) ... in the hydrophobic core, and one containing predominantly polar amino acids oriented toward the solvent-exposed surface of the ... DNA bindingEdit. α-Helices have particular significance in DNA binding motifs, including helix-turn-helix motifs, leucine ...
sequence-specific DNA binding. Cellular component. • PML body. • Нуклеоплазма. • DNA replication factor A complex. • nuclear ... Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". EMBO J. 21 (7): 1855-63. ... The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain". J. Biol. Chem. 273 (7): 3932-6 ... Dornreiter I, Erdile LF, Gilbert IU, von Winkler D, Kelly TJ, Fanning E (1992). „Interaction of DNA polymerase alpha-primase ...
The core of photosystem II consists of two subunits referred to as D1 and D2. These two subunits are similar to the L and M ... give rise to electron transfer reactions along the path of a series of protein-bound co-factors. These co-factors are light- ... They are structurally similar to one another, both having 5 transmembrane alpha helices. Four bacteriochlorophyll b (BChl-b) ... The latter sub-unit is not a general structural motif in photosynthetic bacteria. The L and M subunits bind the functional and ...
An important factor in the binding of the first generation NNRTIs, such as nevirapine, is the butterfly-like shape. Despite ... The cloroindole part interacted with the hydrophobic core of the pocket and influenced the binding mode of the R120393 so it ... Part 1: From alpha-anilinophenylacetamide (alpha-APA) to imidoyl thiourea (ITU)". Bioorganic & Medicinal Chemistry Letters. 11 ... Å from the catalytic site in the palm domain of the p66 subunit site of the enzyme. The NNRTI binding pocket (NNIBP) contains ...
Diagram of the 12 subunit catalytic core of the cubic 24 subunit human E2k. [18] (C) Ribbon diagram of the 8 Monomer E3 subunit ... File:Structure of alpha-KGDHC.jpg Figure 7: Structure of the alpha-KGDHC subunits (A) Ribbon diagram of the homo-dimer ... The first factor was a decrease in transcription (mRNA levels) of the subunits comprising α-KGDHC. As shown in Figure 13, after ... It is an 8 monomer that can be divided into 4 domains; the FAD-binding domain (residues 1-149), the NAD+-binding domain ( ...
These subunits surround the mRNA strand. The larger subunit contains three binding sites: A (aminoacyl), P (peptidyl), and E ( ... Transcription is regulated in the cell via transcription factors. Transcription factors are proteins that bind to regulatory ... The two reacting molecules are the alpha amino group of one amino acid and the alpha carboxyl group of the other amino acids. A ... In a hydrophilic environment such as cytosol, the hydrophobic amino acids will concentrate at the core of the protein, while ...
BDNF is made in the endoplasmic reticulum and secreted from dense-core vesicles. It binds carboxypeptidase E (CPE), and the ... regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits ... receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. Cellular component. • cytoplasm. • ... BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor. ...
Each AMPAR has four sites to which an agonist (such as glutamate) can bind, one for each subunit.[5] The binding site is ... Armstrong N, Sun Y, Chen GQ, Gouaux E (October 1998). "Structure of a glutamate-receptor ligand-binding core in complex with ... "Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin- ... "Postsynaptic factors in the expression of long-term potentiation (LTP): increased glutamate receptor binding following LTP ...
positive regulation of core promoter binding. • interleukin-12-mediated signaling pathway. • regulation of regulatory T cell ... positive regulation of tumor necrosis factor production. • CD8-positive, alpha-beta T cell differentiation involved in immune ... including subunits of the immunoproteasome (MECL1, LMP2, LMP7), as well as TAP and ERAAP in addition possibly to the direct ... However, in contrast to many other heparan sulfate binding proteins, where binding promotes biological activity, the binding of ...
... a 9-subunit base that binds directly to the α ring of the 20S core particle, and a 10-subunit lid. Six of the nine base ... Degradation of Aux/IAA proteins derepresses transcription factors in the auxin-response factor (ARF) family and induces ARF- ... which serve as docking domains for the regulatory particles and the alpha subunits N-termini (Pfam PF10584) form a gate that ... The mechanisms by which it binds to the core particle through the C-terminal tails of its subunits and induces α-ring ...
ubiquitin protein ligase binding. • importin-alpha family protein binding. • disordered domain specific binding. • core ... DNA binding. • core promoter binding. • transcription factor activity, sequence-specific DNA binding. • transcription ... Within each subunit, there are a variety of protein binding sites, as well as a total of 15 possible phosphorylation sites. ... transcription factor binding. • RNA polymerase II activating transcription factor binding. • phosphoprotein binding. • kinase ...
Expression of lymphoid enhancer-binding factor 1 (LEF-1) triggers the translocation of β-catenin to the nucleus, where it ... Protocadherin-alpha and protocadherin-gamma interact to form a protein complex that enhances the surface expression of each ... Davis, MA; Ireton, RC; Reynolds, AB (2003). "A core Function for p120-catenin in cadherin turnover". J. Cell Biol. 163 (3): 525 ... impaired the stabilization of GluR2 and GluR3 AMPAR subunits, respectively, at the plasma membrane. Co-localization of p120ctn ...
phosphopantetheine binding. • 3-oxo-pimeloyl-[acp methyl ester reductase activity]. • RNA binding. • cadherin binding. • 3-oxo- ... In some cancer cell lines, this protein has been found to be fused with estrogen receptor alpha (ER-alpha), in which the N- ... Reevaluation of the side-by-side, antiparallel subunit model". J. Biol. Chem. 274 (17): 11557-63. doi:10.1074/jbc.274.17.11557 ... Metabolism and homeostasis of fatty acid synthase is transcriptionally regulated by Upstream Stimulatory Factors (USF1 and USF2 ...
This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding ... C1QBP has been shown to interact with Protein kinase D1,[10] BAT2,[11] PRKCD,[10] PKC alpha[10] and Protein kinase Mζ.[10] ... "Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation". J Clin ... transcription factor binding. • protein binding. • hyaluronic acid binding. • kininogen binding. • mitochondrial ribosome ...
... being bound by several eukaryotic initiation factors, including eIF1, eIF1A, and eIF3.[6] The 40S ribosomal subunit is also ... This rRNA core is decorated with dozens of proteins. In the figure "Crystal Structure of the Eukaryotic 40S Ribosomal Subunit ... Eukaryote-specific extensions of conserved proteins, ranging from a few residues or loops to very long alpha helices and ... Eukaryotic large ribosomal subunit (60S). References[edit]. *^ 40S+Ribosomal+Subunits at the US National Library of Medicine ...
... binding studies reveal DNA binding specificity mechanisms and functional interplay amongst Forkhead transcription factors". ... POLD1, CDC2, CRCS10, MDPL, POLD, polymerase (DNA) delta 1, catalytic subunit, DNA polymerase delta 1, catalytic subunit. ... The major replicative activity in S phase of cell cycle depends on three DNA polymerases - Polymerase alpha (Polα), Polymerase ... The light blue boxes represent interactions of the core complex. The light pink boxes represent other putative interactions in ...
transcription factor activity, sequence-specific DNA binding. • transcription factor binding. • RNA polymerase II core promoter ... 2009). "The correlation of hepatocyte nuclear factor 4 alpha and 3 beta with hepatitis B virus replication in the liver of ... "Foxa2 and MafA regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression". J. Mol. ... transcription regulatory region DNA binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. ...
"Structure of the RPA trimerization core and its role in the multistep DNA-binding mechanism of RPA". EMBO J. (England) 21 (7): ... "The RPA32 subunit of human replication protein A contains a single-stranded DNA-binding domain.". J. Biol. Chem. 273 (7): 3932- ... "Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.". EMBO J. 11 (2): 769-76. ... "An interaction between the DNA repair factor XPA and replication protein A appears essential for nucleotide excision repair." ...
Each LT-α/LT-β subunit is a trimer and assembles into homotrimers or heterotrimers. LT-α binds with LT-β to form membrane-bound ... January 1999). "Lymphotoxin alpha/beta and tumor necrosis factor are required for stromal cell expression of homing chemokines ... Zhu M, Fu YX (November 2011). "The role of core TNF/LIGHT family members in lymph node homeostasis and remodeling". ... In the LT-α mediated signaling pathway, LT-α binds with LT-β to form the membrane-bound LT-α1-β2 complex. Binding of LT-α1-β2 ...
... caused by the alpha decay of actinium-227.[35] Perey then attempted to determine the proportion of beta decay to alpha decay in ... Gottschlich, Michele M. (2001). The Science and Practice of Nutrition Support: A Case-based Core Curriculum. Kendall Hunt. p. ... The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... Sodium tetraphenylborate can also be classified as an organosodium compound since in the solid state sodium is bound to the ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
protein kinase binding. • core promoter binding. • RNA polymerase II transcription coactivator activity. • transcription factor ... ribosomal large subunit binding. • protein homodimerization activity. • ribosomal small subunit binding. • unfolded protein ... regulation of eIF2 alpha phosphorylation by dsRNA. • positive regulation of cell cycle G2/M phase transition. • negative ... activating transcription factor binding. • rRNA binding. • protein N-terminus binding. • chromatin binding. ...
BDNF is made in the endoplasmic reticulum and secreted from dense-core vesicles. It binds carboxypeptidase E (CPE), and the ... regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits ... receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ... BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor. ...
The alpha helical anticodon binding domain of Arginyl, Glycyl and Cysteinyl-tRNA synthetases is known as the DALR domain after ... Alignment of the core domains of aminoacyl-tRNA synthetases class I and class II. Essential binding site residues (Backbone ... It aminoacylates at the 3'-OH of a terminal adenosine on tRNA, and is usually dimeric or tetrameric (two or four subunits, ... and an anticodon binding domain (which interacts mostly with the anticodon region of the tRNA and ensures binding of the ...
... s can bind to other proteins as well as to small-molecule substrates. When proteins bind specifically to other copies of ... Kauzmann W (May 1956). "Structural factors in protein denaturation". Journal of Cellular Physiology. 47 (Suppl 1): 113-31. doi: ... Quaternary structure: the structure formed by several protein molecules (polypeptide chains), usually called protein subunits ... The membrane alone has a hydrophobic core through which polar or charged molecules cannot diffuse. Membrane proteins contain ...
... the alpha helix). Linus Pauling was the first to identify[29] the 3.6 amino acids per helix turn ratio of the alpha helix. ... It, however, requires a five base binding between the mRNA and tRNA with a flip of the anti-codon creating a triplet coding, ... Such a code might be "degenerate", with 4×4×4=64 possible triplets of the four nucleotide subunits while there were only 20 ... In 1960, Crick accepted an honorary fellowship at Churchill College, Cambridge, one factor being that the new college did not ...
... the position where the substrate binds, may influence factors like binding affinity of ligands, stabilization of substrates ... The number of sub-units making up cellulosomes can also determine the rate of enzyme activity.[12] ... McCleary BV (November 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ... construction of the catalytic core, expression, and X-ray structure at 1.75 Å resolution". Biochemistry. 36 (51): 16032-9. doi: ...
Each tropomyosin molecule has a smaller calcium-binding protein called troponin bound to it. All thin filaments are attached to ... The filaments are organized into repeated subunits along the length of the myofibril. These subunits are called sarcomeres. ... They run through the core of each thick filament and anchor it to the Z-line, the end point of a sarcomere. Titin also ... Troponin and the associated tropomyosin undergo a conformational change after calcium binding and expose the myosin binding ...
Each subunit consists of three domains. At the carboxyl terminal of the peptide chain there's a short alpha helix domain that ... oxygen binding. • metal ion binding. • dopamine binding. • enzyme binding. • oxidoreductase activity. • iron ion binding. • ... response to growth factor. • response to ethanol. • cellular response to glucose stimulus. • phthalate metabolic process. • ... allows tetramerization.[15] The central ~300 amino acids make up a catalytic core, in which all the residues necessary for ...
... is the gene encoding the ε subunit of DNA polymerase III in Escherichia coli.[1] The ε subunit is one of three core ... of the gene product binds the θ subunit and carries out the exonuclease function and the C-terminus binds the α subunit ... "The proofreading exonuclease subunit epsilon of Escherichia coli DNA polymerase III is tethered to the polymerase subunit alpha ... The ε subunit is stabilized by the θ subunit within the complete polymerase complex.[7] ...
14 alpha particles) is easily produced from lighter nuclei in the alpha process in nuclear reactions in supernovae (see silicon ... The inner core of the Earth is generally presumed to consist of an iron-nickel alloy with ε (or β) structure.[10] ... Hemoglobin bound to carbon monoxide is known as carboxyhemoglobin. This effect also plays a minor role in the toxicity of ... The ensuing availability of inexpensive iron was one of the factors leading to the Industrial Revolution. Toward the end of the ...
InaD contains five binding domains called PDZ domain proteins, which specifically bind the C termini of target proteins. ... Drosophila differentially expresses a core group of 252 genes upon infection with most bacteria. This core group of genes is ... Bakker K (1961). "An analysis of factors which determine success in competition for food among larvae of Drosophila ... A 2016 study concluded that food supplementation with 10-mM alpha-ketoglutarate decreased Drosophila alcohol sensitivity over ...
The activated Gs alpha subunit binds to and activates an enzyme called adenylyl cyclase, which, in turn, catalyzes the ... crystallised the PKA Cα subunit, which revealed the bi-lobe structure of the protein kinase core for the very first time, ... Long term memory is dependent on the CREB transcription factor, regulated by PKA. A study done on drosophila reported that an ... Two cAMP molecules bind to each PKA regulatory subunit. *The regulatory subunits move out of the active sites of the catalytic ...
In the presence of the R subunit, the complex can also act as an endonuclease, binding to the same target sequence but cutting ... A conserved enzyme core with variable target-recognizing domains". J. Mol. Biol. 206 (2): 305-12. doi:10.1016/0022-2836(89) ... hsdM contains an alpha-helical domain at the N-terminus, the HsdM N-terminal domain.[7] ... The type I restriction and modification system is composed of three polypeptides R, M and S. The M (hsdM) and S subunits ...
Most vegetable oils are rich in linoleic acid (safflower, sunflower, and corn oils). Alpha-linolenic acid is found in the green ... Malinauskas T (2008). "Docking of fatty acids into the WIF domain of the human Wnt inhibitory factor-1". Lipids. 43 (3): 227-30 ... The steroids, all derived from the same fused four-ring core structure, have different biological roles as hormones and ... Polyketides are synthesized by polymerization of acetyl and propionyl subunits by classic enzymes as well as iterative and ...
The same protein may act as the subunit of both the pentamers and hexamers or they may be composed of different proteins.[81]. ... When the adaptive immune system of a vertebrate encounters a virus, it produces specific antibodies that bind to the virus and ... Counting these areas and multiplying by the dilution factor allowed him to calculate the number of viruses in the original ... Safety, tolerability and efficacy of peginterferon alpha-2a and ribavirin in chronic hepatitis C in clinical practice: The ...
Assembly factor proteins[31] 47. NDUFAF1c. CIA30_HUMAN. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex, assembly factor 1. ... Core Subunitsa 1. NDUFS7 / PSST / NUKM. NDUS7_HUMAN. NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial EC ... Complex I contains a ubiquinone binding pocket at the interface of the 49-kDa and PSST subunits. Close to iron-sulfur cluster ... 1 alpha subcomplex subunit 3. Pfam PF14987 39. NDUFA4 / MLRQc. NDUA4_HUMAN. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex ...
X-ray crystallographic structure of the 30S ribosomal subunit with bound drug (purple, space-filling model, at center) protein ... Volume 1. Core Clinical Skills. Oxford University Press. 2010. *^ referenced in Treatment of Mitochodrial Disease: Bindu LH, ... PubMed:19031229] See Also Fischel-Ghodsian N. Genetic factors in aminoglycoside toxicity. Ann NY Acad Sci. 1999; 884:99-109. [ ... secondary structure elements such as alpha-helices in bright green, and the RNA phosphodiester backbone shown in orange (and ...
SL3/AKV core binding factor alpha A subunit. *SL3/AKV core-binding factor alpha A subunit ... Core binding factor. *Core binding factor runt domain alpha subunit 1. *Core binding factor subunit alpha 1 ... CBF binds to the core site, 5-PYGPYGGT-3, of a number of enhancers and promoters, including murine leukemia virus, ... Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of ...
... alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2, (10ug), 10 µg. ... Home » cDNA » Mouse cDNA » Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 ( ... MC219266 Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), ... Properties for Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) ( ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related ... The protein produced from the normal RUNX1 gene is part of a protein complex known as core binding factor (CBF). As part of CBF ... Core binding factor acute myeloid leukemia. A rearrangement (translocation) of genetic material involving the RUNX1T1 gene is ... Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. Int J Hematol. 2011 Aug;94(2): ...
... signalling molecules and transcription factors. Definitive HSCs derived during embryogenesis in the aorta-gonad-mesonephros ... Core Binding Factor Alpha 2 Subunit * Proto-Oncogene Proteins c-myb * Zebrafish Proteins ... Haematopoietic stem cell (HSC) homeostasis is tightly controlled by growth factors, signalling molecules and transcription ... factors. Definitive HSCs derived during embryogenesis in the aorta-gonad-mesonephros region subsequently colonize fetal and ...
Core Binding Factor Alpha 2 Subunit * DNA-Binding Proteins / genetics* * DNA-Binding Proteins / metabolism ... Most of the RUNX1 mutations are clustered in the Runt domain and result in defective DNA binding but active beta-subunit ... binding, which is consistent with three-dimensional structural findings and may explain the dominant inhibitory effects. Unlike ...
... while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of ... The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus ... CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding ... RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5-TGTGGT ...
SL3-3 enhancer factor 1 alpha A subunit. *SL3/AKV core-binding factor alpha A subunit ... The RUNX2 protein is a transcription factor, which means it attaches (binds) to specific regions of DNA and helps control the ... 2013 Feb 7;92(2):252-8. doi: 10.1016/j.ajhg.2012.12.001. Epub 2013 Jan 3. Citation on PubMed or Free article on PubMed Central ... 2012 Mar;18(2):184-90. doi: 10.1111/j.1601-0825.2011.01862.x. Epub 2011 Oct 24. Citation on PubMed ...
Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (Predicted). ... PREDICTED: transcription initiation factor TFIID subunit 4-like[Cricetulus griseus].. 11. Mus musculus. 12396. Cbfa2t2; ... PREDICTED: transcription initiation factor TFIID subunit 4B-like[Cricetulus griseus].. 10. Cricetulus griseus. 100769929. ... PREDICTED: transcription initiation factor TFIID subunit 4-likeisoform 1 [Bombus impatiens].. 31. Pteropus vampyrus. ...
Core Binding Factor Alpha 1 Subunit); 0 (Drugs, Chinese Herbal); 0 (RUNX2 protein, human). ... Subunidade alfa 1 de Fator de Liga o ao Core/gen tica. Seres Humanos. Sistema de Sinaliza o das MAP Quinases/efeitos dos f ... 0 (Enzyme Inhibitors); EC 3.2.1.1 (alpha-Amylases); EC 3.4.21.62 (Subtilisin). ... Prote na Morfogen tica ssea 2/gen tica. Diferencia o Celular/efeitos dos f rmacos. C lulas Cultivadas. ...
Compare CBFA2/RUNX1 translocation partner 2 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... Core Binding Factor Alpha Subunit 2 Translocated To 2 (. *. Detection Range: 0.313 ng/ml - 20 ng/ml ... Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target... read more ... CBFA2/RUNX1 translocation partner 2 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody- ...
CBFA2/RUNX1 Translocation Partner 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... core binding factor Runt domain,alpha subunit 2,(RUNX1) translocated to 2,mainly expressed in brain,with several isoforms due ... Core-binding factor, runt domain, alpha subunit 2; translocated to, 2:. *Hs.153934 ... Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 2 2 3 ...
CBFA2/RUNX1 Partner Transcriptional Co-Repressor 2, including: function, proteins, disorders, pathways, orthologs, and ... core binding factor Runt domain,alpha subunit 2,(RUNX1) translocated to 2,mainly expressed in brain,with several isoforms due ... Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 2 2 3 ... Transcription Factor. Binding Sites. Gene Targets. GH20J033400. Promoter/Enhancer. 2.1. EPDnew Ensembl ENCODE CraniofacialAtlas ...
AML2SL3/AKV core-binding factor alpha C subunit. *CBFA3. *CBFA3MGC16070. *CBF-alpha-3 ...
AML2SL3/AKV core-binding factor alpha C subunit. *CBFA3. *CBFA3MGC16070. *CBF-alpha-3 ...
Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Assay Type: Probe Assay Design: Exonic Application: Gene ... Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Assay Type: SYBR® Green Assay Design: Exonic Application ... Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting ... R2. ,0.99. These DNA primer pairs were designed by prioritizing the gene regions most commonly found in transcript variants. ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 3. *Omim ID: ... and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi ... MTG8-related gene 2,myeloid translocation gene on chromosome 16,zinc finger MYND domain-containing protein 4 ...
... alpha subunit 2; translocated to, 2) for WB. Anti-MTGR1 pAb (GTX16137) is tested in Human samples. 100% Ab-Assurance. ... core-binding factor, runt domain, alpha subunit 2; translocated to, 2. Background. Myeloid tumor related gene (MTGR1) is found ... For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple ...
Core-binding factor subunit alpha-1; Short=CBF-alpha-1;AltName: Full=Oncogene AML-3;AltName: Full=Osteoblast-specific ... the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. Virology. 1993 May;194(1):314-31 ... J:19082 Ogawa E, et al., PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila ... J:49076 Xiao ZS, et al., Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor. ...
Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: SYBR® Green Assay Design: exonic ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Assay Design: exonic ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Application: Gene Expression ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Application: Gene Expression ...
core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (human) ... CBFA2T2 identified gene homolog,OTTMUSP00000016821,core-binding factor runt domain alpha subunit 2 translocated to 2 homolog, ... core-binding factor, runt domain, alpha subunit 2, translocated to, 2 homolog ... Novel binding partners of Ldb1 are required for haematopoietic development.. Meier N, Krpic S, Rodriguez P, Strouboulis J, ...
core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human). ... ccr4-not transcription complex, subunit 3. Cops2 12848. cop9 (constitutive photomorphogenic) homolog, subunit 2 (arabidopsis ... cbp/p300-interacting transactivator, with glu/asp-rich carboxy-terminal domain, 2. ...
SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor ... DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding transcription factor binding ... Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; ... regulatory region DNA binding RNA polymerase II regulatory region sequence-specific DNA binding core promoter binding ...
Core Binding Factor Alpha 2 Subunit. *MicroRNAs. *DNA-Binding Proteins. *FISH. *TCF3 ... bHLH transcription factor binding - cell development - chromatin binding - cytoplasm - DNA binding - E-box binding - G1 phase ... transcription factor binding - transcription factor complex - transcription regulatory region DNA binding - transcription, DNA- ... sequence-specific DNA binding - sequence-specific DNA binding transcription factor activity - T cell differentiation in thymus ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Calculated molecular weight: ... CBFA2T3, ETO2, hMTG16, MTG16, MTG8 related protein 2, MTGR2, Protein CBFA2T3, ZMYND4 ...
Acute Disease; Amino Acid Sequence; Animals; Base Sequence; Cell Differentiation; Core Binding Factor Alpha 2 Subunit; DNA, ... Complementary; DNA-Binding Proteins/classification; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology*; Disease ... Transcription Factors/classification; Transcription Factors/genetics; Transcription Factors/physiology*; Transgenes; Zebrafish/ ... runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl, and expression of ...
Core Binding Factor Alpha 2 Subunit/metabolism; Female; Gene Expression Regulation; Hematopoiesis*; Hematopoietic Stem Cell ... Transcription Factor AP-1/metabolism; Transcription, Genetic; Zebrafish/embryology* ...
Mouse runt-related transcription factor 2 ELISA Kit-NP_001139510.1 (MBS934869) product datasheet at MyBioSource, ELISA Kits ... CBF-alpha-1; PEA2-alpha A; PEBP2 alpha A; AKV core binding factor; core binding factor alpha 1; runt domain, alpha subunit 1; ... PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit. ... CBF-alpha 1; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A; runt-related transcription factor 2 ...
Core Binding Factor Alpha 1 Subunit * Assays * Lymphoid Enhancer-Binding Factor 1 ...
2. Fast two directional hand movements 3. Recommended for drawing curved hair stro ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (CBFA2T3), transcript variant 2 ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4 ... Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. ...
Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription ... factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Can repress the ... cDNA FLJ33666 fis, clone BRAMY2027752, highly similar to Homo sapiens core-binding factor, runt domain, alpha subunit 2; ... cDNA FLJ33666 fis, clone BRAMY2027752, highly similar to Homo sapiens core-binding factor, runt domain, alpha subunit 2; ...
Core-binding factor subunit alpha-1. FDP:. Fixed dental prostheses. RUNX2:. Runt-related transcription factor 2 ... a transcription factor on chromosome 6p21, have been identified as the cause [3]. This transcription factor is responsible for ... factors such as risk of chipping, esthetics [34], and costs must be weighed. In this case, no ceramic-veneered FDPs were ... 2. a The OPG and b, c excerpts from the CBCT scan. Typical CCD findings can be seen: retained teeth, jaw malposition, and bony ...
  • It performs this function by attaching (binding) to proteins that normally turn genes on and blocking their activity. (medlineplus.gov)
  • Guo C, Hu Q, Yan C, Zhang J. Multivalent binding of the ETO corepressor to E proteins facilitates dual repression controls targeting chromatin and the basal transcription machinery. (medlineplus.gov)
  • RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. (thermofisher.com)
  • E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. (cancerindex.org)
  • The specificity of tissue-restricted gene therapy can be refined by utilization of a 31-amino-acid segment of the hematopoietic and osteogenic AML/CBF transcription factors that direct the regulatory proteins to subnuclear sites that support gene expression. (umassmed.edu)
  • Sequences that support the intranuclear trafficking of AML/CBF transcription factors to subnuclear sites that support transcription were determined by the expression and visualization of mutated and epitope tagged AML/CBF proteins. (umassmed.edu)
  • Nuclear proteins that are localized in discrete domains within the nucleus include the leukemia-associated acute myelogenous leukemia (AML) and promyelocytic leukemia (PML) factors, the SC-35 RNA-processing factors, nucleolar proteins and components of both transcriptional and DNA replication complexes. (umassmed.edu)
  • Additionally we are shipping RUNX1T1 Proteins (6) and RUNX1T1 Kits (2) and many more products for this protein. (antibodies-online.com)
  • RUNX proteins are evolutionarily conserved transcription factors known to be involved in various developmental processes. (elsevier.com)
  • In studies on murine polyoma and leukemia viruses, mammalian core-binding factors (CBFs) were identified as proteins regulating viral replication (Boral et al. (springer.com)
  • Proteins belonging to the MADS family function as dimers, the primary DNA-binding element of which is an anti-parallel coiled coil of two amphipathic alpha-helices, one from each subunit. (ebi.ac.uk)
  • Full gene expression occurs when transcription activator proteins bind to each module within the regulatory promoter. (wikipedia.org)
  • These proteins are known as CCAAT box binding proteins/CCAAT box binding factors. (wikipedia.org)
  • Top-down proteomics is dependent upon high resolution and accurate mass measurements for analysis of intact proteins and their dissociation-derived product ions and can provide complete primary structure and post-translational modification assignment [ 1 , 2 ]. (pubmedcentralcanada.ca)
  • This protein found to be tightly associated with membrane-bound ribosomes, either directly or through adaptor proteins. (wikipedia.org)
  • At the core, the bHLH-PAS transcriptional activators CLOCK and BMAL1 activate the Period ( Per1, Per2 ) and Cryptochrome ( Cry1, Cry2 ) genes, whose transcripts and proteins slowly accumulate during the daytime. (hhmi.org)
  • Under oxidative stress, NRF2 heterodimerizes with small Maf proteins and binds cis-acting enhancer sequences found near oxidative stress response genes. (hindawi.com)
  • RUNX proteins form a heterodimeric complex with CBFβ which confers increased DNA binding and stability to the complex. (hitchhikersgui.de)
  • The transcription of RUNX1 is regulated by 2 enhancers (regulatory element 1 and regulatory element 2), and these tissue specific enhancers enable the binding of lymphoid or erythroid regulatory proteins, therefore the gene activity of RUNX1 is highly active in the haematopoietic system. (hitchhikersgui.de)
  • Promoters contain specific DNA sequences such as response elements that provide a secure initial binding site for RNA polymerase and for proteins called transcription factors that recruit RNA polymerase. (wikipedia.org)
  • A promoter is induced in response to changes in abundance or conformation of regulatory proteins in a cell, which enable activating transcription factors to recruit RNA polymerase. (wikipedia.org)
  • Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. (antikoerper-online.de)
  • The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. (genecards.org)
  • PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene. (jax.org)
  • One of the genes that encodes a CBF alpha subunit is AML1, also called Cbf alpha 2. (curehunter.com)
  • RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. (uniprot.org)
  • The subnuclear organization of nucleic acids and cognate regulatory factors suggests that there are functional interrelationships between nuclear structure and gene expression. (umassmed.edu)
  • Mechanisms that control the spatial distribution of transcription factors within the three-dimensional context of the nucleus may involve the sorting of regulatory information, as well as contribute to the assembly and activity of sites that support gene expression. (umassmed.edu)
  • Molecular, cellular, genetic and biochemical approaches have identified distinct protein segments, termed intranuclear-targeting signals, that are responsible for directing regulatory factors to specific subnuclear sites. (umassmed.edu)
  • Gene rearrangements that remove or alter intranuclear-targeting signals are prevalent in leukemias and have been linked to altered localization of regulatory factors within the nucleus. (umassmed.edu)
  • Investigating the regulatory mechanism of viral replication led to the identification of the polyomavirus enhancer-binding protein 2 (PEBP2), also known as core-binding factor-β (CBF-β) (Katinka et al. (springer.com)
  • RUNX2 is essential for osteoblastic differentiation and skeletal morphogenesis, and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. (thefreedictionary.com)
  • Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2). (labome.org)
  • Identification of IFN regulatory factor-1 binding site in IL-12 p40 gene promoter. (labome.org)
  • The beta subunit is the regulatory subunit which allosterically enhances the activity of the DNA binding alpha subunit as the complex binds to the core site of various enhancers and promoters. (diagenode.com)
  • 2. A recombinant nucleic acid molecule comprising a promoter sequence operably linked to a nucleic acid molecule according to claim 1. (google.es)
  • A sigma factor is the promoter specificity subunit of eubacterial-type multisubunit RNA polymerases, those whose core subunit composition is often described as alpha(2)-beta-beta-prime. (cathdb.info)
  • Although sigma does not bind DNA on its own, when combined with the core to form the holoenzyme, this binds specifically to promoter sequences, with the sigma factor making sequence specific contacts with the promoter elements. (cathdb.info)
  • Any process involved in the assembly of the RNA polymerase preinitiation complex (PIC) at the core promoter region of a DNA template, resulting in the subsequent synthesis of RNA from that promoter. (cathdb.info)
  • the promoter lacks one of the two C residues from the pentameric motif, and the artificial addition of the second C has no significant effects on binding activity. (wikipedia.org)
  • The CAAT box is what is known as a core promoter, also known as the basal promoter or simply the promoter, is a region of DNA that initiates transcription of a particular gene. (wikipedia.org)
  • In an experiment done with core binding factors (CBF) and DNA complexes, researchers were able to determine the preferential sequences of the promoter in a region over and immediately adjacent to the CAAT box, and two regions on either side of the CAAT box. (wikipedia.org)
  • The gene can be transcribed from 2 alternative promoters , promoter 1 (distal) or promoter 2 (proximal). (hitchhikersgui.de)
  • There is no lactose to inhibit the repressor , so the repressor binds to the operator , which obstructs the RNA polymerase from binding to the promoter and making lactase. (wikipedia.org)
  • Lactose is inhibiting the repressor, allowing the RNA polymerase to bind with the promoter and express the genes, which synthesize lactase. (wikipedia.org)
  • The promoter is recognized by RNA polymerase and an associated sigma factor , which in turn are often brought to the promoter DNA by an activator protein's binding to its own DNA binding site nearby. (wikipedia.org)
  • The process is more complicated, and at least seven different factors are necessary for the binding of an RNA polymerase II to the promoter. (wikipedia.org)
  • Forms the heterodimeric complex core-binding factor (CBF) with CBFB. (uniprot.org)
  • Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. (trademetro.net)
  • The five genes were DIRAS3 (DIRAS family, GTP-binding RAS-like 3), CXCL6 (chemokine (C-X-C motif) ligand 6), SAMD5 (sterile alpha motif domain containing 5), CBFB (core-binding factor, beta subunit), and MEIS2 (meis homeobox 2). (molvis.org)
  • 1982 ). Further study revealed that PEBP2/CBFB was composed of dimers of a DNA-binding subunit (CBF-α) and a non-DNA-binding subunit (CBF-β). (springer.com)
  • Polyclonal antibody raised in rabbit against human CBFb (core-binding factor, beta subunit) using two KLH-conjugated synthetic peptides containing sequences from the central region of the protein. (diagenode.com)
  • CBFb (UniProtKB/Swiss-Prot entry Q13951) represents the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family. (diagenode.com)
  • The RUNX2 protein is a transcription factor, which means it attaches (binds) to specific regions of DNA and helps control the activity of particular genes. (medlineplus.gov)
  • Parathyroid Hormone-responsive Smad3-related Factor, Tmem119, Promotes Osteoblast Differentiation and Interacts with the Bone Morphogenetic Protein-Runx2 Pathway. (jax.org)
  • Foxo1 mediates insulin-like growth factor 1 (IGF1)/insulin regulation of osteocalcin expression by antagonizing Runx2 in osteoblasts. (jax.org)
  • MBS934869 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. (mybiosource.com)
  • Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. (mybiosource.com)
  • To identify genes whose expressions in primary human trabecular meshwork (TM) cell cultures are affected by the transcription factor pituitary homeobox 2 (PITX2) and to identify genes that may have roles in glaucoma. (molvis.org)
  • Testing for multiple genes in a panel using NGS platforms is more practical and economical (2,3). (thefreelibrary.com)
  • These specialized sigma factors bind the promoters of genes appropriate to the environmental conditions, increasing the transcription of those genes. (wikipedia.org)
  • σ 70 (RpoD) - σ A - the "housekeeping" sigma factor or also called as primary sigma factor , transcribes most genes in growing cells. (wikipedia.org)
  • Every cell has a "housekeeping" sigma factor that keeps essential genes and pathways operating. (wikipedia.org)
  • These chromosomal abnormalities tend to disrupt genes that encode for transcription factors needed for myeloid stem cells to differentiate into specific blood components. (the-medical-dictionary.com)
  • SRF function is essential for transcriptional regulation of numerous growth-factor-inducible genes, such as c-fos oncogene and muscle-specific actin genes. (ebi.ac.uk)
  • Two enhancers at the TCRalphadelta locus, the TCR alpha enhancer and the TCR delta enhancer (Edelta), are responsible for orchestrating the distinct developmental programs for V(D)J recombination and transcription of the TCR alpha and delta genes, respectively. (duke.edu)
  • Activated NRF2 bound the antioxidant response element (ARE) in promoters of several known and novel target genes involved in iron homeostasis and heme metabolism, including known targets FTL and FTH1 , as well as novel binding in the globin locus control region. (hindawi.com)
  • NRF2 (encoded by nuclear factor-erythroid p45-related factor 2, NFE2L2 ) is the master regulator of antioxidant and phase II detoxification genes that collectively resist cellular damage due to electrophilic and oxidative stress [ 1 , 2 ]. (hindawi.com)
  • Figure 2 shows the results of the complete chromosome 3 and three genomic regions region surrounding the OGG1, FUT7 and NFE2 genes, respectively. (diagenode.com)
  • These transcription factors regulate a host of genes specific to haematopoiesis (e.g. (diagenode.com)
  • [8] It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). (hitchhikersgui.de)
  • A redox factor protein, ref1, is involved in negative gene regulation by extracellular calcium. (labome.org)
  • This transcription factor is responsible for the differentiation of precursor cells into osteoblasts and regulates the differentiation of chondrocytes in the growth plate [ 4 ]. (springer.com)
  • Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. (abcam.com)
  • Differentiation of naive CD4+ T cells into helper T (Th) cells is controlled by a combination of several transcriptional factors. (nih.gov)
  • Human serum response factor (SRF) is a ubiquitous nuclear protein important for cell proliferation and differentiation. (ebi.ac.uk)
  • Molecular cloning and characterization of PEBP2 beta, the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. (jax.org)
  • Cloning, mapping and expression of PEBP2 alpha C, a third gene encoding the mammalian Runt domain. (springer.com)
  • Edelta function depends critically on transcription factors core binding factor (CBF)/polyoma enhancer-binding protein 2 (PEBP2) and c-Myb as measured by transcriptional activation of transiently transfected substrates in Jurkat cells, and by activation of V(D)J recombination within chromatin-integrated substrates in transgenic mice. (duke.edu)
  • Our data indicate that CBF/PEBP2 plays primarily a structural role as it induces a conformational change in the enhanceosome that is associated with augmented binding of c-Myb. (duke.edu)
  • In contrast, c-Myb has no apparent affect on CBF/PEBP2 binding, but is critical for transcriptional activation. (duke.edu)
  • Every molecule of RNA polymerase holoenzyme contains exactly one sigma factor subunit, which in the model bacterium Escherichia coli is one of those listed below. (wikipedia.org)
  • RNA polymerase holoenzyme complex consisting of core RNA polymerase and a sigma factor executes transcription of a DNA template strand. (wikipedia.org)
  • The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). (uniprot.org)
  • [1] It is a bacterial transcription initiation factor that enables specific binding of RNA polymerase to gene promoters . (wikipedia.org)
  • Selection of promoters by RNA polymerase is dependent on the sigma factor that associates with it. (wikipedia.org)
  • Experiments in many laboratories have shown that mutations to the CCAAT motif that cause a loss of CBF binding also decreases transcriptional activity in these promoters, suggesting that CBF-CCAAT complexes are essential for optimum transcriptional activity. (wikipedia.org)
  • Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. (genetex.com)
  • These transcription factors have specific activator or repressor sequences of corresponding nucleotides that attach to specific promoters and regulate gene expression. (wikipedia.org)
  • The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. (genetex.com)
  • The protein encoded by this gene belongs to the SECY/SEC61- alpha family. (wikipedia.org)
  • Distinct roles for c-Myb and core binding factor/polyoma enhancer-binding protein 2 in the assembly and function of a multiprotein complex on the TCR delta enhancer in vivo. (duke.edu)
  • PREDICTED: transcription initiation factor TFIID subunit 4B-like[Cricetulus griseus]. (bioinformatics.org)
  • PREDICTED: transcription initiation factor TFIID subunit 4-likeisoform 1 [Bombus impatiens]. (bioinformatics.org)
  • Domain structure of a human general transcription initiation factor, TFIIF. (labome.org)
  • A sigma factor ( σ factor ) is a protein needed only for initiation of transcription. (wikipedia.org)
  • Once initiation of RNA transcription is complete, the sigma factor can leave the complex. (wikipedia.org)
  • This region, in particular for the CAAT box, is located about 60-100 bases upstream (towards the 5' end), however no less than 27 base pairs away, from the initial transcription site or a eukaryote gene in which a complex of general transcription factors bind with RNA polymerase II prior to the initiation of transcription. (wikipedia.org)
  • RUNX3 suppresses gastric epithelial cell growth by inducing p21(WAF1/Cip1) expression in cooperation with transforming growth factor {beta}-activated SMAD. (springer.com)
  • Transcription factor Runx3 regulates interleukin-15-dependent natural killer cell activation. (springer.com)
  • RUNX3 inhibits the expression of vascular endothelial growth factor and reduces the angiogenesis, growth, and metastasis of human gastric cancer. (springer.com)
  • The CLOCK protein acts as a heterodimeric transcription factor with a partner known as BMAL1 (ARNTL). (hhmi.org)
  • RUNX1T1 encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. (antibodies-online.com)
  • Role of transforming growth factor beta in human disease. (springer.com)
  • This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. (wikipedia.org)
  • These modifications in the intranuclear targeting of transcription factors might abrogate fidelity of gene expression in tumor cells by influencing the spatial organization and/or assembly of machineries involved in the synthesis and processing of gene transcripts. (umassmed.edu)
  • Within metazoa (animal kingdom), the core binding factor (CBF)-DNA complex retains a high degree of conservation within the CCAAT binding motif, as well as the sequences flanking this pentameric motif. (wikipedia.org)
  • Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. (medlineplus.gov)
  • Kageyama R, Sasai Y, Nakanishi S. Molecular characterization of transcription factors that bind to the cAMP responsive region of the substance P precursor gene. (labome.org)
  • Sigma factors are distinguished by their characteristic molecular weights. (wikipedia.org)
  • For example, σ 70 is the sigma factor with a molecular weight of 70 kDa . (wikipedia.org)
  • Thus, highly enriched GFP-positive AGM HSCs will serve as a basis for the future examination of the cellular and molecular factors involved in the induction and expansion of adult HSCs. (ox.ac.uk)
  • To understand the molecular mechanisms for synergy between these transcription factors in the context of chromatin, we used in vivo footprinting to study the requirements for protein binding to Edelta within wild-type and mutant versions of a human TCR delta minilocus in stably transfected Jurkat cells. (duke.edu)
  • [2] The specific sigma factor used to initiate transcription of a given gene will vary, depending on the gene and on the environmental signals needed to initiate transcription of that gene. (wikipedia.org)
  • The number of sigma factors varies between bacterial species. (wikipedia.org)
  • [1] [4] E. coli has seven sigma factors. (wikipedia.org)
  • Different sigma factors are utilized under different environmental conditions. (wikipedia.org)
  • [1] In the case of E. coli and other gram-negative rod-shaped bacteria, the "housekeeping" sigma factor is σ 70 . (wikipedia.org)
  • σ 32 (RpoH) - the heat shock sigma factor, it is turned on when the bacteria are exposed to heat. (wikipedia.org)
  • There are also anti-sigma factors that inhibit the function of sigma factors and anti-anti-sigma factors that restore sigma factor function. (wikipedia.org)
  • The sigma subunit is released from the elongating form of the polymerase and is thus free to act catalytically for multiple RNA polymerase core enzymes. (cathdb.info)
  • The presence of specific genetic mutations could aid clinicians in 4 ways: 1) diagnostic algorithms, 2) prognosis assessment, 3) use of targeted therapy, and 4) monitoring treatment response and residual disease. (thefreelibrary.com)
  • Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and transcription corepressor activity . (genecards.org)
  • Kikuchi Y, Yasue T, Miyake K, Kimoto M, Takatsu K. CD38 ligation induces tyrosine phosphorylation of Bruton tyrosine kinase and enhanced expression of interleukin 5-receptor alpha chain: synergistic effects with interleukin 5. (labome.org)
  • These data indicate that CD38 ligation increases IL-5 receptor alpha expression and synergizes with IL-5 to enhance Blimp1 expression and IgM synthesis. (labome.org)
  • translocated to, 3 (CBFA2T3), transcript variant 2 with C terminal Flag and His tag. (trademetro.net)
  • Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. (wikipedia.org)
  • It is homologous to archaeal transcription factor B and to eukaryotic TFIIB . (wikipedia.org)
  • SRF-like/Type I subfamily of MADS (MCM1, Agamous, Deficiens, and SRF (serum response factor)) box family of eukaryotic transcriptional regulators [ PMID: 7744019 ]. (ebi.ac.uk)
  • A core domain of around 90 amino acids is sufficient for the activities of DNA-binding, dimerisation and interaction with accessory factors. (ebi.ac.uk)
  • Among the exons are two defined domains, namely the runt homology domain (RHD) or the runt domain (exons 2, 3 and 4), and the transactivation domain (TAD) (exon 6). (hitchhikersgui.de)