Core Binding Factors: Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor alpha Subunits: A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor Alpha 1 Subunit: A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.Core Binding Factor beta Subunit: A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor Alpha 2 Subunit: A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.Core Binding Factor Alpha 3 Subunit: A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain.Transcription Factor AP-2: A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.Smooth Muscle Myosins: Myosin type II isoforms found in smooth muscle.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Chromosomes, Human, Pair 16: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Chromosome Inversion: An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Oncogene Proteins, Fusion: The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Chromosomes, Human, Pair 21: A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Enhancer Elements, Genetic: Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.Translocation, Genetic: A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Osteocalcin: Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Transforming Growth Factor alpha: An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Leukemia, Myeloid: Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.Pol1 Transcription Initiation Complex Proteins: Factors that form a preinitiation complex at promoters that are specifically transcribed by RNA POLYMERASE I.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibodies, Monoclonal: Antibodies produced by a single clone of cells.Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.Antibodies, Neutralizing: Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.Biotin: A water-soluble, enzyme co-factor present in minute amounts in every living cell. It occurs mainly bound to proteins or polypeptides and is abundant in liver, kidney, pancreas, yeast, and milk.Drosophila: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.Drosophila melanogaster: A species of fruit fly much used in genetics because of the large size of its chromosomes.Epitopes: Sites on an antigen that interact with specific antibodies.Hybridomas: Cells artificially created by fusion of activated lymphocytes with neoplastic cells. The resulting hybrid cells are cloned and produce pure MONOCLONAL ANTIBODIES or T-cell products, identical to those produced by the immunologically competent parent cell.Fluorescent Antibody Technique: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.

Three distinct domains in TEL-AML1 are required for transcriptional repression of the IL-3 promoter. (1/859)

A cytogenetically cryptic (12;21) translocation is the most common molecular abnormality identified in childhood acute lymphoblastic leukemia (ALL), and it generates a chimeric TEL-AML1 protein. Fusion of the Helix-Loop-Helix (HLH) (also called the pointed) domain of TEL to AML1 has been suggested to convert AML1 from a transcriptional activator to a repressor. To define the structural features of this chimeric protein required for repression, we analysed the transcriptional activity of a series of TEL-AML1 mutants on the AML1-responsive interleukin-3 (IL-3) promoter, a potentially relevant gene target. Our results demonstrate that TEL-AML1 represses basal IL-3 promoter activity in lymphoid cells, and deletion mutant analysis identified three distinct domains of TEL-AML1 that are required for repression; the HLH (pointed) motif contained in the TEL portion of TEL-AML1, and both the runt homology domain (Rhd) and the 74 amino acids downstream of the Rhd that are present in the AML1 portion of the fusion protein. Although AML1B (and a shorter AML1 isoform, AML1A) have transcriptional activating activity on the IL-3 promoter, fusion of the AML1 gene to the TEL gene generates a repressor of IL-3 expression. Consistent with this activity, freshly isolated human ALL cells that contain TEL-AML1 do not express IL-3.  (+info)

Biallelic and heterozygous point mutations in the runt domain of the AML1/PEBP2alphaB gene associated with myeloblastic leukemias. (2/859)

The AML1 gene encoding the DNA-binding alpha-subunit in the Runt domain family of heterodimeric transcription factors has been noted for its frequent involvement in chromosomal translocations associated with leukemia. Using reverse transcriptase-polymerase chain reaction (RT-PCR) combined with nonisotopic RNase cleavage assay (NIRCA), we found point mutations of the AML1 gene in 8 of 160 leukemia patients: silent mutations, heterozygous missense mutations, and biallelic nonsense or frameshift mutations in 2, 4, and 2 cases, respectively. The mutations were all clustered within the Runt domain. Missense mutations identified in 3 patients showed neither DNA binding nor transactivation, although being active in heterodimerization. These defective missense mutants may be relevant to the predisposition or progression of leukemia. On the other hand, the biallelic nonsense mutants encoding truncated AML1 proteins lost almost all functions examined and may play a role in leukemogenesis leading to acute myeloblastic leukemia.  (+info)

Mutual activation of Ets-1 and AML1 DNA binding by direct interaction of their autoinhibitory domains. (3/859)

The transcription factors Ets-1 and AML1 (the alphaBl subunit of PEBP2/CBF) play critical roles in hematopoiesis and leukemogenesis, and cooperate in the transactivation of the T cell receptor (TCR) beta chain enhancer. The DNA binding capacity of both factors is blocked intramolecularly but can be activated by the removal of negative regulatory domains. These include the exon VII domain for Ets-1 and the negative regulatory domain for DNA binding (NRDB) for alphaB1. Here we report that the direct interaction between the two factors leads to a reciprocal stimulation of their DNA binding activity and activation of their transactivation function. Detailed mapping revealed two independent contact points involving the exon VII and NRDB regions as well as the two DNA binding domains. Using deletion variants and dominant interfering mutants, we demonstrate that the interaction between exon VII and NRDB is necessary and sufficient for cooperative DNA binding. The exon VII and NRDB motifs are highly conserved in evolution yet deleted in natural variants, suggesting that the mechanism described is of biological relevance. The mutual activation of DNA binding of Ets and AML1 through the intermolecular interaction of autoinhibitory domains may represent a novel principle for the regulation of transcription factor function.  (+info)

A novel ubiquitin-specific protease, UBP43, cloned from leukemia fusion protein AML1-ETO-expressing mice, functions in hematopoietic cell differentiation. (4/859)

Using PCR-coupled subtractive screening-representational difference analysis, we have cloned a novel gene from AML1-ETO knockin mice. This gene is highly expressed in the yolk sac and fetal liver of the knockin mice. Nucleotide sequence analysis indicates that its cDNA contains an 1,107-bp open reading frame encoding a 368-amino-acid polypeptide. Further protein sequence and protein translation analysis shows that it belongs to a family of ubiquitin-specific proteases (UBP), and its molecular mass is 43 kDa. Therefore, we have named this gene UBP43. Like other ubiquitin proteases, the UBP43 protein has deubiquitinating enzyme activity. Protein ubiquitination has been implicated in many important cellular events. In wild-type adult mice, UBP43 is highly expressed in the thymus and in peritoneal macrophages. Among nine different murine hematopoietic cell lines analyzed, UBP43 expression is detectable only in cell lines related to the monocytic lineage. Furthermore, its expression is regulated during cytokine-induced monocytic cell differentiation. We have investigated its function in the hematopoietic myeloid cell line M1. UBP43 was introduced into M1 cells by retroviral gene transfer, and several high-expressing UBP43 clones were obtained for further study. Morphologic and cell surface marker examination of UBP43/M1 cells reveals that overexpression of UBP43 blocks cytokine-induced terminal differentiation of monocytic cells. These data suggest that UBP43 plays an important role in hematopoiesis by modulating either the ubiquitin-dependent proteolytic pathway or the ubiquitination state of another regulatory factor(s) during myeloid cell differentiation.  (+info)

Regulation of c-fos gene transcription and myeloid cell differentiation by acute myeloid leukemia 1 and acute myeloid leukemia-MTG8, a chimeric leukemogenic derivative of acute myeloid leukemia 1. (5/859)

Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Jurkat T-cells, but not by that of the short form of acute myeloid leukemia 1-MTG8, a chimeric acute myeloid leukemia 1 protein. In 32Dcl3 myeloid cells, stable overexpression of acute myeloid leukemia 1-MTG8 blocked the c-fos gene transcription and cell differentiation, but that of acute myeloid leukemia did not. These data suggest that acute myeloid leukemia 1 and acute myeloid leukemia 1-MTG8 reciprocally regulate the myeloid cell differentiation, possibly by the way of regulating c-fos gene transcription.  (+info)

Solution properties of the free and DNA-bound Runt domain of AML1. (6/859)

The Runt domain is responsible for specific DNA and protein-protein interactions in a family of transcription factors which includes human AML1. Structural data on the Runt domain has not yet become available, possibly due to solubility and stability problems with expressed protein fragments. Here we describe the optimization and characterization of a 140-residue fragment, containing the Runt domain of AML1, which is suitable for structural studies. The fragment of AML1 including amino acids 46-185 [AML1 Dm(46-185)] contains a double cysteine-->serine mutation which does not affect Runt domain structure or DNA-binding affinity. Purified AML1 Dm(46-185) is soluble and optimally stable in a buffer containing 200 mm MgSO4 and 20 mm sodium phosphate at pH 6.0. Nuclear magnetic resonance and circular dichroism spectroscopy indicate that the Runt domain contains beta-sheet, but little or no alpha-helical secondary structure elements. The 45 N-terminal residues of AML1 are unstructured and removal of the N-terminal enhances sequence-specific DNA binding. The NMR spectrum of AML1 Dm(46-185) displays a favorable chemical shift dispersion and resolved NOE connectivities are readily identified, suggesting that a structure determination of this Runt domain fragment is feasible. A titration of 15N-labelled AML1 Dm(46-185) with a 14-bp cognate DNA duplex results in changes in the 15N NMR heteronuclear single quantum coherence spectrum which indicate the formation of a specific complex and structural changes in the Runt domain upon DNA binding.  (+info)

Induction of apoptosis in myeloid leukaemic cells by ribozymes targeted against AML1/MTG8. (7/859)

The translocation (8;21)(q22;q22) is a karyotypic abnormality detected in acute myeloid leukaemia (AML) M2 and results in the formation of the chimeric fusion gene AML1/MTG8. We previously reported that two hammerhead ribozymes against AML1/MTG8 cleave this fusion transcript and also inhibit the proliferation of myeloid leukaemia cell line Kasumi-1 which possesses t(8;21)(q22;q22). In this study, we investigated the mechanisms of inhibition of proliferation in myeloid leukaemic cells with t(8;21)(q22;q22) by ribozymes. These ribozymes specifically inhibited the growth of Kasumi-1 cells, but did not affect the leukaemic cells without t(8;21)(q22;q22). We observed the morphological changes including chromatin condensation, fragmentation and the formation of apoptotic bodies in Kasumi-1 cells incubated with ribozymes for 7 days. In addition, DNA ladder formation was also detected after incubation with ribozymes which suggested the induction of apoptosis in Kasumi-1 cells by the AML1/MTG8 ribozymes. However, the ribozymes did not induce the expression of CD11b and CD14 antigens in Kasumi-1 cells. The above data suggest that these ribozymes therefore inhibit the growth of myeloid leukaemic cells with t(8;21)(q22;q22) by the induction of apoptosis, but not differentiation. We conclude therefore that the ribozymes targeted against AML1/MTG8 may have therapeutic potential for patients with AML carrying t(8;21)(q22;q22) while, in addition, the product of the chimeric gene is responsible for the pathogenesis of myeloid leukaemia.  (+info)

Expression of AML1-d, a short human AML1 isoform, in embryonic stem cells suppresses in vivo tumor growth and differentiation. (8/859)

The human AML1 gene encodes a heterodimeric transcription factor which plays an important role in mammalian hematopoiesis. Several alternatively spliced AML1 mRNA species were identified, some of which encode short protein products that lack the transactivation domain. When transfected into cells these short isoforms dominantly suppress transactivation mediated by the full length AML1 protein. However, their biological function remains obscure. To investigate the role of these short species in cell proliferation and differentiation we generated embryonic stem (ES) cells overexpressing one of the short isoforms, AML1-d, as well as cells expressing the full length isoforms AML1-b and AML2. The in vitro growth rate and differentiation of the transfected ES cells were unchanged. However, overexpression of AML1-d significantly affected the ES cells' ability to form teratocarcinomas in vivo in syngeneic mice, while a similar overexpression of AML1-b and AML2 had no effect on tumor formation. Histological analysis revealed that the AML1-d derived tumors were poorly differentiated and contained numerous apoptotic cells. These data highlight the pleiotropic effects of AML1 gene products and demonstrate for the first time an in vivo growth regulation function for the short isoform AML1-d.  (+info)

TY - JOUR. T1 - Prognostic value of AML 1/ETO fusion transcripts in patients with acute myelogenous leukemia.. AU - Cho, Eun Kyung. AU - Bang, Soo Mee. AU - Ahn, Jeong Yeal. AU - Yoo, Seung Min. AU - Park, Pil Whan. AU - Seo, Yieh Hea. AU - Shin, Dong Bok. AU - Lee, Jae Hoon. PY - 2003/1/1. Y1 - 2003/1/1. N2 - BACKGROUND: The t (8;21) (q22;q22), which produces the fusion gene AML1/ETO, is associated with relatively good prognosis and, in particular, with a good response to cytosine arabinoside. Analysis of t (8;21) positive leukemic blasts has shown characteristic morphological and immunological features. We performed this study to investigate the incidence of AML1/ETO rearrangement in adult acute myelogenous leukemia (AML), especially in M2 subtype, to make a comparison of clinical, morphological and immunophenotypic characteristics between AML1/ETO rearrangement positive and negative group in patients with AML and to analyze the correlation with other biological parameters. METHODS: From May ...
The t(8;21) acute myeloid leukemia (AML)-associated oncoprotein AML1-ETO disrupts normal hematopoietic differentiation. Here, we have investigated its effects on the transcriptome and epigenome in t(8,21) patient cells. AML1-ETO binding was found at promoter regions of active genes with high levels of histone acetylation but also at distal elements characterized by low acetylation levels and binding of the hematopoietic transcription factors LYL1 and LMO2. In contrast, ERG, FLI1, TAL1, and RUNX1 bind at all AML1-ETO-occupied regulatory regions, including those of the AML1-ETO gene itself, suggesting their involvement in regulating AML1-ETO expression levels. While expression of AML1-ETO in myeloid differentiated induced pluripotent stem cells (iPSCs) induces leukemic characteristics, overexpression increases cell death. We find that expression of wild-type transcription factors RUNX1 and ERG in AML is required to prevent this oncogene overexpression. Together our results show that the interplay ...
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Loss of RUNX1/ETO Triggers C/EBPα-Driven Reorganization of the Leukemic Transcriptional Network(A) RUNX1/ETO and CEBPA mRNA expression levels in Kasumi-1 cells
CATAGAGCCA GCGGGCGCGG GCGGGACGGG CGCCCCGCGG CCGGACCCAG CCAGGGCACC ACGCTGCCCG GCCCTGCGCC GCCAGGCACT TCTTTCCGGG ^1 ^11 ^21 ^31 ^41 ^51 ^61 ^71 ^81 ^91 GCTCCTAGGG ACGCCAGAAG GAAGTCAACC TCTGCTGCTT CTCCTTGGCC TGCGTTGGAC CTTCCTTTTT TTGTTGTTTT TTTTTGTTTT TCCCCTTTCT ^101 ^111 ^121 ^131 ^141 ^151 ^161 ^171 ^181 ^191 TCCTTTTGAA TTAACTGGCT TCTTGGCTGG ATGTTTTCAA CTTCTTTCCT GGCTGCGAAC TTTTCCCCAA TTGTTTTCCT TTTACAACAG GGGGAGAAAG ^201 ^211 ^221 ^231 ^241 ^251 ^261 ^271 ^281 ^291 TGCTCTGTGG TCCGAGGCGA GCCGTGAAGT TGCGTGTGCG TGGCAGTGTG CGTGGCAGGA TGTGCGTGCG TGTGTAACCC GAGCCGCCCG ATCTGTTTCG ^301 ^311 ^321 ^331 ^341 ^351 ^361 ^371 ^381 ^391 ATCTGCGCCG CGGAGCCCTC CCTCAAGGCC CGCTCCACCT GCTGCGGTTA CGCGGCGCTC GTGGGTGTTC GTGCCTCGGA GCAGCTAACC GGCGGGTGCT ^401 ^411 ^421 ^431 ^441 ^451 ^461 ^471 ^481 ^491 GGGCGACGGT GGAGGAGTAT CGTCTCGCTG CTGCCCGAGT CAGGGCTGAG TCACCCAGCT GATGTAGACA GTGGCTGCCT TCCGAAGAGT GCGTGTTTGC ^501 ^511 ^521 ^531 ^541 ^551 ^561 ^571 ^581 ^591 ATGTGTGTGA CTCTGCGGCT GCTCAACTCC CAACAAACCA GAGGACCAGC ...
Chromosome changes in the bone marrow (BM) of patients with persistent cytopenia are often considered diagnostic for a myelodysplastic syndrome (MDS). Comprehensive cytogenetic evaluations may give evidence of the real pathogenetic role of these changes in cases with cytopenia without morphological signs of MDS. Chromosome anomalies were found in the BM of three patients, without any morphological evidence of MDS: 1) an acquired complex rearrangement of chromosome 21 in a boy with severe aplastic anaemia (SAA); the rearrangement caused the loss of exons 2-8 of the RUNX1 gene with subsequent hypoexpression. 2) a constitutional complex rearrangement of chromosome 21 in a girl with congenital thrombocytopenia; the rearrangement led to RUNX1 disruption and hypoexpression. 3) an acquired paracentric inversion of chromosome 1, in which two regions at the breakpoints were shown to be lost, in a boy with aplastic anaemia; the MPL gene, localized in chromosome 1 short arms was not mutated neither disrupted, but
The TEL-AML1 fusion not only characterizes the most frequent genetic rearrangement in initial childhood ALL (20-25%) but its presence has also been associated with a favorable prognosis (9, 10, 11 , 28 , 32) . In clinical studies on initial ALL, probability of event-free survival (EFS) at 4 years was as high as 90-100% for TEL-AML1+ patients (9 , 10 , 32) . These results certainly do not represent final outcome considering that, regardless of the different prevalence of TEL-AML1 positivity at relapse of BCP-ALL (range, 3- 28%), TEL-AML1+ leukemia is biologically characterized by a long duration of first CR and that the majority of relapses (80%) occur off-therapy (median, 46 months; range, 13-125 months; Refs. 18, 19, 20, 21, 22, 23 , 33 ). The prevalence of TEL-AML1 positivity in our ongoing prospective study on first relapse of BCP-ALL is ∼17% (31 of 178 children; 33 ).. Obviously, the predictive value of TEL-AML1 positivity alone is insufficient to stratify patients to appropriate treatment ...
Further we asked if VLA-4 and VLA-5 integrin upregulation is maintained by RUNX1/ETO in the transformed human leukemia cell line Kasumi-1, derived from a t(8;21)+ AML patient. Kasumi-1 cells, which express RUNX1/ETO and to a lesser extent RUNX1/ETOtr,4 bear high levels of VLA-4 whereas the integrin αL subunit is absent in these cells. We specifically down-regulated RUNX1/ETO via lentivirally delivered shRNA targeting the RUNX1/ETO breakpoint sequences (shRE), which are present in both full length and truncated forms (Online Supplementary Figure S3). At Day 4 after transduction with vectors co-expressing shRE and eGFP, α4, α5 and β1 expression levels were significantly reduced as assessed using flow cytometry, while CXCR4 levels remained unaltered (Figure 1I). Similar results were obtained with NHR2 competitive peptides (N89) (Figure 1J), which also interfere with both RUNX1/ETO forms by disrupting RUNX1/ETO tetramer formation.6 These results suggest that integrin subunit expression remains ...
MGA is an incompletely studied gene with a high mutation frequency in MLL-PTD AML (9%) and in core bind factor AML (8%). This gene encodes a MAX-interacting protein and is believed to act as a transcription factor that suppresses MYC binding to its target. By in silico analysis, we found that MGA is expressed in normal myeloid hematopoietic cells and AML, and the expression level is comparable with TET2 or DNMT3A. Further data mining of TCGA revealed a high frequency of inactivating mutations of the MGA gene in a variety of cancers such as various adenocarcinomas. To interrogate functionally its role in leukemogenesis, lentiviral constructs containing either shRNA or CRISPR-sgRNA targeted to different regions of the MGA gene were generated. MGA expressing AML cell line EOL-1 was silenced by shRNA or CRISPER system. Silencing was confirmed by western blot (shRNA) and Sanger Sequencing (sgRNA). An increase of methylcellulose colony number (~30%) was observed in MGA silenced cell lines. Control ...
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The mechanism underlying the lineage decision made by CD4(+)CD8(+) double-positive (DP) thymocytes that give rise to two T lymphocyte subset with distinct functionalities, that is, helper and cytotoxic T cells, remains a major issue in immunology. The lineage decision process involves several phases and terminates when cells loose their developmental plasticity to become the alternate lineage. A detailed picture of the transcription factor network governing helper versus cytotoxic-lineage decision has recently emerged. Studies published only past year provided new insights into how the expression of ThPOK, a central transcription factor for helper T cell development, is regulated. It has now become evident that an antagonistic interplay between ThPOK and Runx transcription factor complexes plays an essential role in thwarting an alternate fate during the commitment process.
Scientists from the Cancer Science Institute of Singapore (CSI Singapore) at the National University of Singapore (NUS) have discovered that modifications to a protein called RUNX3 may promote cancer progression. The results of the study were published in the prestigious journal Proceedings of the National Academy of Sciences (PNAS) in June 2016. The research team, led by Professor Yoshiaki Ito, Senior Principal Investigator at CSI Singapore, found that a modification called phosphorylation made to RUNX3 promotes cancer progression by allowing cell division. Uncontrolled cell division in the body is a process by which tumours form and hence is a hallmark of cancer. RUNX3 is a tumour suppressor gene that prevents the formation of tumours by binding to DNA. The phosphorylation, or the addition of a phosphate group to a molecule, is carried out by an enzyme called Aurora Kinase, which has been observed to be present in unusually high levels in some cancers. Phosphorylation prevents the binding of ...
Study hypothesis: Treatment with dasatinib 100 mg QD is safe and efficacious when given to patients with Ph+ ALL in the post SCT setting.
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RUNX1 antibody (runt-related transcription factor 1) for WB. Anti-RUNX1 pAb (GTX11903) is tested in Human, Mouse samples. 100% Ab-Assurance.
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AML-2 (Acute myeloid leukemia 2 or Runx3) antibody against the runt transcription factor 3 for use in supershift (EMSA) and Western blotting.
PHF2라는 단백질이 뼈를 만드는 세포(조골세포)를 활성화시킨다는 사실을 처음으로 규명했다. 조골세포는 Runx2라는 단백질에 의해 분화가 조절된다. 반면, SUV39HI1라는 효소는 Runx2에 메틸기(CH3)를 붙임으로써 Runx2가 기능을 하지 못하게 하는 장식으로 분화를 방해한다. 성장이 끝난 성인들이 더 이상 키가 크지 않는 것도 SUV39HI1 효소 때문이다. 이에 착안해 Runx2에 붙어 있는 메틸기를 제거하는 방안을 연구한 결과, PHF2 단백질이 조골세포 분화를 유도함으로써, 소아의 뼈 발달 과정이나 골절 후 뼈가 새로 형성되는 과정에 작용한다는 것을 증명했다.. PHF2 단백질은 Runx2에 붙어 있는 메틸기를 제거했으며, 이후 본연의 기능을 회복한 Runx2는 조골세포의 분화를 촉진하여 다시 뼈를 만들기 시작했다. 실제 유전자 조작으로 PHF2 단백질이 과발현된 쥐를 만들어 ...
Accountants have been warned to expect an increase in AML compliance work in the wake of a new report criticising bodies for a widespread lack of ML
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Runt-related transcription factor 1 (RUNX1) is generally considered to function as a tumor suppressor in the development of leukemia, but a growing body of evidence suggests that it has pro-oncogenic properties in acute myeloid leukemia (AML). Here we have demonstrated that the antileukemic effect mediated by RUNX1 depletion is highly dependent on a functional p53-mediated cell death pathway. Increased expression of other RUNX family members, including RUNX2 and RUNX3, compensated for the antitumor effect elicited by RUNX1 silencing, and simultaneous attenuation of all RUNX family members as a cluster led to a much stronger antitumor effect relative to suppression of individual RUNX members. Switching off the RUNX cluster using alkylating agent-conjugated pyrrole-imidazole (PI) polyamides, which were designed to specifically bind to consensus RUNX-binding sequences, was highly effective against AML cells and against several poor-prognosis solid tumors in a xenograft mouse model of AML without ...
Runt-related transcription factor 1 (RUNX1) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters and can accelerate apoptosis in various tumors. However, the regulatory mechanisms underlying RUNX1 expression in neuroblastoma (NB), a highly malignant tumor in childhood, remain largely unclear. In this study, we aimed to assess the role of RUNX1 in NB and to reveal the underlying mechanisms that may contribute to finding a potential therapeutics strategy against NB. Growth, invasion, metastasis and angiogenesis were assessed using Cell Counting Kit-8 (CCK-8) immunocytochemistry, and studies involving soft agar, cell invasion, tube formation and whole animals. The levels of expression were measured using real-time quantitative PCR for RNA, Western blot and immunostaining analyses for proteins. Luciferase reporter and chromatin immunoprecipitation assays indicated that RUNX1 directly binds within the BIRC5, CSF2RB and NFKBIA promoter regions to facilitate
Hematopoietic stem cells (HSCs) are self-renewing multipotent stem cells that generate mature blood lineages throughout life. They, together with hematopoietic progenitor cells (collectively known as HSPCs), emerge from hemogenic endothelium in the floor of the embryonic dorsal aorta by an endothelial-to-hematopoietic transition (EHT). Here we demonstrate that transforming growth factor β (TGFβ) is required for HSPC specification and that it regulates the expression of the Notch ligand Jagged1a in endothelial cells prior to EHT, in a striking parallel with the epithelial-to-mesenchymal transition (EMT). The requirement for TGFβ is two fold and sequential: autocrine via Tgfβ1a and Tgfβ1b produced in the endothelial cells themselves, followed by a paracrine input of Tgfβ3 from the notochord, suggesting that the former programs the hemogenic endothelium and the latter drives EHT. Our findings have important implications for the generation of HSPCs from pluripotent cells in vitro.
BACKGROUND: Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously as oncoproteins and tumor suppressors to leukemia and other cancers. To discover fundamental Runx functions in the cell biology of animal development, we have employed morpholino antisense-mediated knockdown of the sea urchin Runx protein SpRunt-1. Previously we showed that embryos depleted of SpRunt-1 arrest development at early gastrula stage and underexpress the conventional protein kinase C SpPKC1. RESULTS: We report here that SpRunt-1 deficiency leads to ectopic cell proliferation and extensive apoptosis. Suppression of the apoptosis by pharmacological inhibition of caspase-3 prevents the ectopic proliferation and rescues gastrulation, indicating that many of the overt defects obtained by knockdown of SpRunt-1 are secondary to the apoptosis. Inhibition or knockdown of SpPKC1 also causes apoptosis, while cell survival is rescued in SpRunt-1 morphant embryos coinjected with
The Runt related transcription factors (RUNX) are recognized as key players in suppressing or promoting tumor growth. RUNX3, a member of this family, is known as a tumor suppressor in many types of cancers, although such a paradigm was challenged by some researchers. The TGF-β pathway governs major upstream signals to activate RUNX3. RUNX3 protein consists of several regions and domains. The Runt domain is a conserved DNA binding domain and is considered as the main part of RUNX proteins since. Herein, we compared the effects of Runt domains and full-Runx3 in cell viability by designing two constructs of Runx3, including N-terminal region and Runt domain. We investigated the effect of full-Runx3, N-t, and RD on growth inhibition in AGS, MCF-7, A549, and HEK293 cell lines which are different in TGF-β sensitivity, in the absence and presence of TGF-β. The full length RUNX3 did not notably inhibit growth of these cell lines while, the N-t and RD truncates showed different trends in these cell lines.
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The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
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The algorithm predicts the chance of the achievement of a complete remission and the risk for an early death for elderly patients (60 years and older) with newly diagnosed AML.
Looking for online definition of core-binding factor, runt domain, alpha subunit 3 in the Medical Dictionary? core-binding factor, runt domain, alpha subunit 3 explanation free. What is core-binding factor, runt domain, alpha subunit 3? Meaning of core-binding factor, runt domain, alpha subunit 3 medical term. What does core-binding factor, runt domain, alpha subunit 3 mean?
The Caenorhabditis elegans run gene encodes a Runt domain factor. Runx1, Runx2, and Runx3 are the three known mammalian homologs of run. Runx1, which plays an essential role in hematopoiesis, has been identified at the breakpoint of chromosome translocations that are responsible for human leukemia. Runx2 plays an essential role in osteogenesis, and inactivation of one allele of Runx2 is responsible for the human disease cleidocranial dysplasia. To understand the role of run in C. elegans, we used transgenic run::GFP reporter constructs and a double-stranded RNA-mediated interference method. The expression of run was detected as early as the bean stage exclusively in the nuclei of seam hypodermal cells and lasted until the L3 stage. At the larval stage, expression of run was additionally detected in intestinal cells. The regulatory elements responsible for the postembryonic hypodermal seam cells and intestinal cells were separately located within a 7.2-kb-long intron region. This is the first ...
core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. Few prognostic markers have been identified. In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. Overall, 10-year overall survival (OS), disease-free survival (DFS), and event-free survival were 63.9%, 54.8%, and 49.9%, respectively; patients with the t(8;21) and inv(16) chromosomal rearrangements exhibited significant differences at diagnosis. Despite similarly high complete remission (CR) rate, patients with inv(16) experienced superior DFS and a high chance of achieving a second CR, often leading to prolonged OS also after relapse. We found that a complex karyotype (ie, ≥4 cytogenetic anomalies) affected survival; the KIT D816 mutation predicted worse prognosis only in patients with the t(8;21) rearrangement, whereas FLT3 mutations had no ...
The nuclear matrix protein, NMP-2, was originally identified as an osteoblast-specific DNA-binding complex localized exclusively to the nuclear matrix. NMP-2 was shown to recognize two binding sites, site A (nt-605 to -599) and site B (nt -441 to -435), in the rat bone-specific osteocalcin gene promoter. This study shows that the NMP-2 binding sites A and B as well as a third NMP-2 binding site (nt -135 to -130) constitute a consensus sequence, ATGCTGGT, and represent an AML-1 recognition motif. AML-1 is a member of the AML transcription factor family which is associated with acute myelogenous leukemia and binds to the sequence TGCTGGT via its DNA-binding runt domain. Electrophoretic mobility shift assays reveal that a component of NMP-2 is a member of the AML/PEBP2/runt domain transcription factor family based on cross-competition with AML-1 consensus oligonucleotide. Limited immunoreactivity of NMP-2 with a polyclonal N-terminal AML-1 antibody and inability of the AML-1 partner protein CBF-beta to
Cell proliferation. To assess cell proliferation, 1 × 105 cells of the indicated AML-derived cells were seeded in 6-well plates. For the tetracycline-inducible gene or shRNA expression, doxycycline was added to the culture at a final concentration of 3 μM. Trypan blue dye exclusion assays were performed every other day.. RT-qPCR. Total RNA was isolated with an RNeasy Mini Kit (Qiagen) and reverse transcribed with a ReverTra Ace kit (TOYOBO) to generate cDNA. RT-qPCR was carried out with a 7500 Real-Time PCR System (Applied Biosystems) according to the manufacturers instructions. The results were normalized to GAPDH levels. Relative expression levels were calculated using the 2-ΔΔCt method. Primers used for RT-qPCR are listed in Supplemental Table 3.. ChIP-qPCR. ChIP was performed using a SimpleChIP Plus Enzymatic Chromatin IP Kit (Cell Signaling Technology) according to the manufacturers instructions. In brief, cells were cross-linked in 1% formaldehyde in PBS for 10 minutes at room ...
Estrogen receptor α (ER α) and androgen receptor (AR) are master transcription factors in the breast and prostate, respectively. They are commonly known in development of sexual characteristics. However, both ERα and AR have been known to be involved in breast cancer (BCa) and prostate cancer (PCa) progression, respectively. The Runx family of transcription factors plays a role in hematopoiesis (Runx1), skeletogenesis (Runx2) and neurogenesis (Runx3). In addition, Runx proteins inhibit cell cycle progression, and have been assigned tumor suppressor roles in various contexts. Because both BCa and PCa cells metastasize to bone at high frequency, investigators have interrogated the possibility that they share characteristics with osteoblasts. Indeed, BCa and PCa cells were found to have "osteomimetic" properties, including expression of Runx2 and Runx2-target genes otherwise expressed by osteoblasts. Provoked by the reported physical interaction between AR and Runx2, we initiated a study to test ...
In order to ascertain the effectiveness of nutrient rich diet and dietary counseling on the health status of pediatric acute lymphoblastic leukemia patients, this experimental study was conducted at the Institute of Radiology and Nuclear Medicine, Peshawar. A sample of 30 leukemia patients were divided into experimental and control groups based on written consents. Data regarding demographic characteristics, anthropometric measurements, and retrospective food intakes were recorded on self-constructed questionnaire. Patients in the experimental group received dietary guidelines for nutrient rich diet. Anthropometry, dietary evaluation, and blood nutrients namely serum ferritin, albumin, globulin, total protein and creatinine at 30, 60 and 90 days intervals were assessed. The data showed low height for age and low weight or height at diagnosis indicating malnourishment and wasting among all the patients. After nutritional intervention mean weights of patients in the experimental group increased ...
ASSESSMENT OF OBESITY AND HEPATIC LATE ADVERSE EFFECTS IN THE EGYPTIAN SURVIVORS OF PEDIATRIC ACUTE LYMPHOBLASTIC LEUKEMIA: SINGLE CENTER STUDY
ASSESSMENT OF OBESITY AND HEPATIC LATE ADVERSE EFFECTS IN THE EGYPTIAN SURVIVORS OF PEDIATRIC ACUTE LYMPHOBLASTIC LEUKEMIA: SINGLE CENTER STUDY
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
van der Deen M, Taipaleenmaki H, Zhang Y, Teplyuk NM, Gupta A, Cinghu S, Shogren K, Maran A, Yaszemski MJ, Ling L, Cool SM, Leong DT, Dierkes C, Zustin J, Salto-Tellez M, Ito Y, Bae SC, Zielenska M, Squire JA, Lian JB, Stein JL, Zambetti GP, Jones SN, Galindo M, Hesse E, Stein GS, van Wijnen AJ. MicroRNA-34c inversely couples the biological functions of the runt-related transcription factor RUNX2 and the tumor suppressor p53 in osteosarcoma. J Biol Chem. 2013 Jul 19; 288(29):21307-19. Epub 2013 May 29 ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Among patients with good prognosis core binding factor AML, there is an overall survival rate of only 44%. To understand the genetic factors contributing to poor outcomes within this subgroup, Dr Chew is analysing bone marrow samples collected from 18 patients before and during treatment.. According to Dr Chew, multiple genetic abnormalities acquired during therapy are probably responsible for good prognosis core binding AML developing resistance to chemotherapy.. "To help us predict who will respond poorly to therapy, were identifying the genetic mutations occurring in patients who relapse," he said. "This information will allow us to tailor patient treatment accordingly. Currently a stem cell transplant is considered the definitive treatment and our findings will help clinicians decide if their patients AML will develop resistance and if a stem cell transplant is recommended.". Dr Chew is testing the usefulness of the genetic variations he identifies through an international ...
Days that platelet count firstly rebound to 75×10^9/L, 100×10^9/L, respectively, from the first time below 75×10^9/L at the chemotherapy cycle and the last chemotherapy ...
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Dagmara Beines daughter Zuza was given an 8% - 15% chance of surviving when she was diagnosed with a particularly aggressive form of AML
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We have shown that autophagic degradation regulates both the basal turnover and the therapy-induced elimination of the aggregate-prone oncoprotein PML/RARA, associated with acute promyelocytic leukemia (APL). This study also revealed an important role of autophagy in promoting granulocytic differentiation of APL cells (Isakson et al, Blood 2010). However, this does not seem to be a general feature of leukemic fusion oncoproteins, as we recently found that AML1-ETO, the most frequently occurring acute myeloid leukemia (AML) fusion protein, is not an autophagy substrate. Rather we demonstrate a clear pro-survival role of autophagy in this AML subtype and that addition of autophagy inhibitors in the treatment regimen might be beneficial ...
Dasatinib is an inhibitor of multiple tyrosine kinases. It is marketed as Sprycel for the treatment of chronic myeloid leukemia (CML). It is also indicated for the treatment of adults with Philadelphia chromosome- positive acute lymphoblastic leukemia.
Clofarabine (injection) is approved by the Food and Drug Administration (FDA) for the treatment of pediatric patients 1 to 21 years old with relapsed ac
Schuler, F., Afreen, S., Manzl, C., Häcker, G., Erlacher, M.,Villunger, A. Checkpoint kinase 1 is essential for fetal and adult hematopoiesis. EMBO Reports. 2019; e47026. doi:10.15252/embr.201847026. ...
Lets say the armrest is broken in your vintage car. As things stand, you would need a lot of luck and persistence to find the right spare part. But in the world of Industrie 4.0 and production with batch sizes of one, you can simply scan the armrest and print it out. This is made possible by the first ever 3D scanner capable of working autonomously and in real time. The autonomous scanning system will be on display at the Hannover Messe Preview on February 6 and at the Hannover Messe proper from April 23 to 27, 2018 (Hall 6, Booth A30). ...
Expression of RUNX1T1 (AML1T1, CBFA2T1, CDR, ETO, MTG8, ZMYND2) in placenta tissue. Antibody staining with HPA070951 in immunohistochemistry.
RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding…
Xp3 (panel A), GZMB (panel B), RUNX3 (panel C) immunostaining. *P,0,05 vs NM; { P,0,05 vs CRC. RUNX3 level in CD8+ T cells coexpressing (white bars) and not
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Improved early event-free survival with imatinib in Philadelphia chromosome - Positive acute lymphoblastic leukemia: A Childrens Oncology Group Study Academic Article ...
Even in the tyrosine kinase inhibitor era, allogeneic hematopoietic stem cell transplantation (HSCT) is regarded as standard care for adult Philadelphia (Ph) positive acute lymphoblastic leukemia (ALL). In this retrospective national study, we have reviewed the outcome after HSCT in Sweden for adult Ph-positive ALL between 2000 and 2009. In total, 51 patients with median age 42 (range 20-66) years underwent HSCT. Mainly allogeneic HSCT was performed (24 related donor, 24 unrelated donor and one cord blood), and only two patients were treated with an autologous HSCT. The 5-year OS was 51 (37-64) %. The probabilities of morphological relapse and non-relapse mortality (NRM) at 5 years were 36 (23-49) and 18 (9-29) %, respectively. For the allogeneic transplanted, the 5-year OS was for patients ,40 years 70 (50-90) % and for patients ,= 40 years 34 (16-52) %, p = 0.002. The 5-year probability of NRM was for patients ,40 years 10 (2-28) % compared to 25 (11-42) % for patients ,= 40 years (p = 0.04). ...
TY - JOUR. T1 - HDAC1 is a required cofactor of CBFb-SMMHC and a potential therapeutic target in inversion 16 acute myeloid leukemia. AU - Richter, Lisa E.. AU - Wang, Yiqian. AU - Becker, Michelle E.. AU - Coburn, Rachel A.. AU - Williams, Jacob T.. AU - Amador, Catalina. AU - Hyde, R. Katherine. PY - 2019/6/1. Y1 - 2019/6/1. N2 - Acute myeloid leukemia (AML) is a neoplastic disease characterized by the uncontrolled proliferation and accumulation of immature myeloid cells. A common mutation in AML is the inversion of chromosome 16 [inv (16)], which generates a fusion between the genes for core binding factor beta (CBFB) and smooth muscle myosin heavy chain gene (MYH11), forming the oncogene CBFB-MYH11. The expressed protein, CBFb-SMMHC, forms a heterodimer with the key hematopoietic transcription factor RUNX1. Although CBFb-SMMHC was previously thought to dominantly repress RUNX1, recent work suggests that CBFb-SMMHC functions together with RUNX1 to activate transcription of specific target ...
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We investigated the expression differences of the TEL-AML1 fusion gene in a leukemia glucocorticoid (GC)-sensitive cell line (CEM) and a GC-resistant cell line (Jurkat). Changes in TEL-AML1 expression before and after GC exposure were analyzed. Expression of GC-sensitive and GC-resistant leukemia cells following initial diagnosis and during treatment was simulated. Leukemia cells were divided into a GC-unexposed or a GC-exposed group.
Platelet disorders lead to defects in primary hemostasis and produce signs and symptoms different from coagulation factor deficiencies (disorders of secondary hemostasis). The bodys reaction to v... more
The objective of this proposal was to identify factors that allow blood vessels to generate hematopoietic stem cells early in the embryonic stage. The process of blood generation from vessels is a normal step in development, but it is poorly understood. We predicted that precise information related to the operational factors in the embryo would allow us to reproduce this process in a petri dish and generate hematopoietic stem cells when needed (situations associated with blood transplantation or cancer). In the second year of this proposal, we have made significant progress and identified critical factors that are responsible for the generation of hematopoietic stem cells from the endothelium (inner layer of blood vessels). These experiments were performed in mouse embryos, as it would be impossible do achieve this goal in human samples. The genes identified are not novel, but have not been associated with this capacity previously. To verify our findings we have independently performed ...
Hello, my wife is currently suffering from Acute Tubular necrosis of the kidneys and in addition her platelet count keeps falling requiring platelets to be iv given. In addition she has over 15Kg ...
This project explores the role of a group of molecules that dictate how the shape of cells influences progression of cancer in white blood cells.
Complete information for CBFB gene (Protein Coding), Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Leading AML expert Dr. Uma Borate goes back to the basics to explain how AML presents itself in the body and the recommended testing at diagnosis. Watch now to learn more.
Additive antitumor activity of dasatinib and Eto in MDA-MB-231 cells. The cells were treated with indicated concentrations of dasatinib and/or 0.1 μM Eto for t
Bleeding from gums often results in infection, injury or inflammation of the gums which is a serious Health issue. Bleeding starts when the plaque is removed in adequate amount from the teeth gum line. Continuous gum bleeding might result in serious infection such as leukemia and bleeding and platelet disorders and it is caused by bacteria ...
Expression of RUNX3 (AML2, CBFA3, PEBP2A3) in vagina tissue. Antibody staining with HPA059006 and CAB025416 in immunohistochemistry.
Cell-Autonomous Function of Runx1 Transcriptionally Regulates Mouse Megakaryocytic Maturation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Reasonably designed. Risk-based. Mitigating controls. Adopted and approved. Fully implemented. Effectively managed. Inherent and residual. Appropriate.. We all know these terms and hear them day-in and day-out in the world of AML. But how many of us truly understand how they relate to AML risk assessment? When I first came to the land of AML following the events of 9-11, I asked myself, "How do we know what the risks are without taking a careful inventory of who we are, what we offer, where and to whom we offer it, and how we deliver it?" Seems simple enough on the face of it.. Every day, as individuals and consumers, we encounter many ways in which we navigate risk and make decisions, and some of our decisions pose higher risk than others.. Remember when you were applying for that pre-approved mortgage and you were told what amount you qualified for? Many thought, "Oh, I must be able to afford that." Not so fast! How many mortgage owners are feeling the brunt of this now? Did you cross against ...
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The level of inflammotin of each subject should be measured before and after receiving the dosage. This experiment should be done daily for a total of two weeks. ...
Runx1 mediates the development of the granular convoluted tubules in the submandibular glands[1] "The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription factor (RUNX) proteins (RUNX1, 2 and 3). This result clearly demonstrates that the Runx /Cbfb signaling pathway is indispensable in the development of the GCTs. However, it is not clear which of the RUNX proteins plays useful role in the development of the GCTs by activating the Runx /Cbfb signaling pathway. Past studies have revealed that the Runx /Cbfb signaling pathway plays important roles in various aspects of development and homeostatic events. Moreover, the Runx genes have different temporospatial ...
Hematopoietic stem cells (HSCs) are the cells that can give rise to all different lineages of differentiated/mature blood cells. HSCs are capable of self-renewal and maintaining the homeostasis of differentiated cells for specific functions. The adult type HSCs were first identified in the para-aortic splanchnopleura/Aorta-Gonad-Mesonephros (PSp/AGM) region, the vitelline and umbilical arteries, yolk sac, and placenta. The current hypothesis is that HSCs differentiate from a "hemogenic endothelium". Core binding factors (CBFs), Runx1 and CBFβ, are essential for the generation of definitive hematopoiesis. We hypothesized that Runx1-CBFβ function is specifically required in the hemogenic endothelium per se for HSC emergence. To test this hypothesis, we selectively disrupted or supplied Runx1-CBFβ function in endothelial cells. Specifically, we conditionally deleted Runx1 in endothelial cells with Cre recombinases expressed in the endothelium (Tek-Cre and VE-cadherin-Cre) combined with a floxed ...
Treatment for Childhood Acute Lymphoblastic Leukemia in Sewri, Mumbai. Find Doctors Near You, Book Appointment, Consult Online, View Doctor Fees, Address, Phone Numbers and Reviews. Doctors for Childhood Acute Lymphoblastic Leukemia in Sewri, Mumbai | Lybrate
Authors: Nigel A Morrison, Alexandre A Stephens, Motomi Osato, Patsie Polly, Timothy C Tan, Namiko Yamashita, James D Doecke, Julie Pasco, Nicolette Fozzard, Graeme Jones, Stuart H Ralston, Philip N Sambrook, Richard L Prince, Geoff C Nicholson
We identified a novel fusion gene, BMP2K-ZNF384, in addition to the previously reported ZNF384-related fusion genes, including TCF3-, TAF15-, EWSR-, EP300-, "CREBBP-", "SYNRG-", and ARID1B-ZNF384, and thus 8 fusion partners for the ZNF384 gene have been identified so far.9-15,19-22 Considering the current condition, whereby the frequency of B-others in which specific cytogenetic abnormalities are not present are markedly decreasing, the incidence of ZNF384-related fusion genes in our cohort was unexpectedly high. In the literature, 8 ALL patients with TAF15-ZNF384 have been reported.9,19-21 Similarly, 2 and 3 ALL patients with EWSR1- and TCF3-ZNF384, respectively, have also been described previously.9-10,20-22 Because 6 out of 13 patients in the literature are adults (,21 years), this is the first report on the high frequency of the recurrence of ZNF384-related fusion genes in childhood BCP-ALL in a single nation. It is possible that the differences in the outbreak frequency of ZNF384-related ...
The transcription factor RUNX1/AML1 is an important regulator of hematopoiesis and RUNX1 gene is one of the most frequent target of chromosomal translocations in cells of the myeloid lineage [1]. Interestingly, the RUNX1 gene covers 260 kbp of chromosome 21 but surprisingly, all genomic breakpoints for the leukemia causing translocations (8; 21) and (16;21) are found in intron 5 of the gene [2]. Presently, factors involved in maintaining the structural integrity and/or enhancing susceptibility of these regions to undergo recombination are unknown. Moreover, the breakpoint junctions are devoid of common DNA motifs that can explain the high recombination frequency observed. Interestingly however, topoisomerase II and DNase I hypersensitive sites have been found to correlate with breakpoints suggesting that chromatin organization may be responsible for, or contribute to, chromosomal translocation formation [3, 4]. DNA regions that exhibit DNase I hypersensitivity have been extensively associated ...
Platelet disorders can involve either a decreased number of platelets (thrombocytopenia) or defective platelet function. Functional disorders of platelets can be inherited (rare) or acquired (common).... more
Decreased Llgl1 expression has been previously shown to be associated with increased metastatic potential, malignant phenotype, or inferior survival in a variety of solid tumors (Ohali et al., 2004; Schimanski et al., 2005; Kuphal et al., 2006). Given that Llgl1 restrains self-renewal in HSCs and that leukemia is characterized by aberrant activation of self-renewal, we assessed for a role in human AML. First, we asked whether the gene expression signature revealed by genetic inactivation of Llgl1 (Llgl1−/− signature) in HSCs was associated with any genetic subtypes of primary AML. Indeed, clustering based on the Llgl1−/− signature (Fig. 4 B) grouped AML cases into subgroups significantly associated with cytogenetic abnormalities (Fig. 4, B and C). Especially core-binding factor leukemias (t(8;21) and inv(16)) or t(15;17) clustered into specific subgroups. Moreover, the Llgl1−/− signature was most strongly correlated with group 7 based on hierarchical clustering. This group contained ...
Platelets help wounds heal and prevent bleeding by forming blood clots. Learn about problems from having too few, too many, or abnormal platelets.
Platelet is disc-shaped, small cellular element in the blood, essential for blood clotting. Normally 200.000-300,000 platelets are found in 1 cubic centimeter of blood. Also called thrombocyte.
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Elmer W.Koneman M.D. is Professor Emeritus, Department of Pathology, University of Colorado School of Medicine. Residing in Breckenridge,CO, Dr. Koneman is a Board Certified Pathologist, former full Professor of Pathology University of Colorado School of Medicine. He is author of numerous journal articles and books.. ...
J:143615 Orelio C, Haak E, Peeters M, Dzierzak E, Interleukin-1-mediated hematopoietic cell regulation in the aorta-gonad-mesonephros region of the mouse embryo. Blood. 2008 Dec 15;112(13):4895-904 ...
A genetic defect found on the GATA2 gene predisposes people to acute myeloid leukemia (AML) and myelodysplasia, researchers have learned.
Similar to most cancers, genome-wide DNA methylation profiles are commonly altered in pediatric acute lymphoblastic leukemia (ALL); however, recent observations highlight that a large portion of malignancy-associated DNA methylation alterations are not accompanied by related gene expression changes. By analyzing and integrating the methylome and transcriptome profiles of pediatric B-cell ALL cases and primary tissue controls, we report 325 genes hypermethylated and downregulated and 45 genes hypomethylated and upregulated in pediatric B-cell ALL, irrespective of subtype. Repressed cation channel subunits and cAMP signaling activators and transducers are overrepresented, potentially indicating a reduced cellular potential to receive and propagate apoptotic signals. Furthermore, we report specific DNA methylation alterations with concurrent gene expression changes within individual ALL subtypes. The ETV6-RUNX1 translocation was associated with downregulation of ASNS and upregulation of the ...
Giant platelet disorders are rare disorders featuring abnormally large platelets, thrombocytopenia and a tendency to bleeding. Giant platelets cannot stick adequately to an injured blood vessel walls, resulting in abnormal bleeding when injured. Giant platelet disorder occurs for inherited diseases like Bernard-Soulier syndrome, gray platelet syndrome and May-Hegglin anomaly. Symptoms usually present from the period of birth to early childhood as: nose bleeds, bruising, and/or gum bleeding. Problems later in life may arise from anything that can cause internal bleeding such as: stomach ulcers, surgery, trauma, or menstruation. Abnormality of the abdomen, nosebleeds, heavy menstrual bleeding, purpura, too few platelets circulating in the blood, and prolonged bleeding time have also been listed as symptoms of various Giant Platelet Disorders. Many of the further classifications of Giant Platelet Disorder occur as a result of being genetically passed down through families as an autosomal recessive ...

*CBFA2T2

"Entrez Gene: CBFA2T2 core-binding factor, runt domain, alpha subunit 2; translocated to, 2". Rual JF, Venkatesan K, Hao T, ... The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several ... 18 (2): 846-58. PMC 108796 . PMID 9447981. Fracchiolla NS, Colombo G, Finelli P, Maiolo AT, Neri A (1998). "EHT, a new member ... 241 (2): 287-95. doi:10.1016/S0378-1119(99)00481-3. PMID 10675041. Hoogeveen AT, Rossetti S, Stoyanova V, Schonkeren J, ...

*CBFA2T3

"Entrez Gene: CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3". Hoogeveen AT, Rossetti S, ... and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi ... makes distinct contacts with multiple histone deacetylases and binds mSin3A through its oligomerization domain". Mol. Cell. ... 18 (2): 846-58. PMC 108796 . PMID 9447981. Gamou T, Kitamura E, Hosoda F, Shimizu K, Shinohara K, Hayashi Y, Nagase T, Yokoyama ...

*RUNX2

... also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that in humans is encoded by the RUNX2 gene. RUNX2 ... The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants ... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for ... The phosphorylation state of Runx2 also mediates it's DNA-binding activity. Interestingly, the Runx2 DNA-binding activity is ...

*RUNX1

... or core-binding factor subunit alpha-2 (CBFA2) is a protein that in humans is encoded by the RUNX1 gene. RUNX1 is a ... It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). ... but its DNA binding affinity is enhanced by 10 fold if it heterodimerises with the core binding factor β (CBFβ), also via the ... RUNX can behave as heterodimeric transcription factors collectively called the core binding factors (CBFs). The consensus ...

*CBFB

... it allosterically enhances DNA binding by the alpha subunit as the complex binds to the core site of various enhancers and ... Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family ...

*Sigma factor

Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. It was previously believed that the RNA ... Sigma factors in E. coli:. *σ70(RpoD) - σA - the "housekeeping" sigma factor or also called as primary sigma factor, ... Different sigma factors are utilized under different environmental conditions. These specialized sigma factors bind the ...

*TAF11

The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ... Transcription initiation factor TFIID subunit 11 also known as TAFII28, is a protein that in humans is encoded by the TAF11 ... The conserved region contains four alpha helices and three loops arranged as in histone H3. TAF11 has been shown to interact ... In molecular biology, TAFII28 refers to the TATA box binding protein associated factor. Together with the TATA-binding protein ...

*GTF2A1L

This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that ... pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding ... TFIIA-alpha and beta-like factor is a protein that in humans is encoded by the GTF2A1L gene. The assembly and stability of the ... "Entrez Gene: ALF TFIIA-alpha/beta-like factor". Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap ...

*Promoter (genetics)

DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... "New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ...

*CAAT box

These core binding factors, or Nuclear Factors (NF-Y), are composed of three subunits - NF-YA, NF-YB, and NF-YC. Whereas in ... The first domain (A1) contains 20 amino acids that forms an alpha helix that appears significant in its interactions with NF-YB ... It is essential to the transcription that these core binding factors (also referred to as nuclear factor Y or NF-Y) are able to ... the core binding factor (CBF)-DNA complex retains a high degree of conservation within the CCAAT binding motif, as well as the ...

*Promoter (genetics)

DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. doi:10.1126/science.8248780. PMID 8248780 ... RNA polymerase holoenzymes containing other sigma factors recognize different core promoter sequences. <-- upstream downstream ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... "Nuclear factor-kappaB-dependent induction of interleukin-8 gene expression by tumor necrosis factor alpha: evidence for an ...

*SEC61B

This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 ... Chen Y, Le Cahérec F, Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... Knight BC, High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...

*Sec61 alpha 1

Chen Y; Le Cahérec F; Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. GRCh38: ... Knight BC; High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ...

*TAF15

The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ... "Structure-function analysis of the estrogen receptor alpha corepressor scaffold attachment factor-B1: identification of a ... This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and ... TATA-binding protein-associated factor 2N is a protein that in humans is encoded by the TAF15 gene. Initiation of transcription ...

*CREB-binding protein

"BRCA1 augments transcription by the NF-kappaB transcription factor by binding to the Rel domain of the p65/RelA subunit". The ... Karetsou Z, Kretsovali A, Murphy C, Tsolas O, Papamarcaki T (April 2002). "Prothymosin alpha interacts with the CREB-binding ... hydrophobic amino acids in the interaction between the glucocorticoid receptor tau 1-core activation domain and target factors ... "Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) ...

*POLR2J

"The alpha-like RNA polymerase II core subunit 3 (RPB3) is involved in tissue-specific transcription and muscle differentiation ... cooperation with promoter-bound activator domains and binding to TFIIB". J. Mol. Biol. 261 (5): 599-606. doi:10.1006/jmbi. ... "HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors". Genes Dev. 6 (4): 655-66. ... The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of ...

*RNA polymerase

... subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, ... RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa, a beta (β) ... The core enzyme has five subunits (~400 kDa):[23] *β': The β' subunit is the largest subunit, and is encoded by the rpoC gene.[ ... In order to bind promoters, RNAP core associates with the transcription initiation factor sigma (σ) to form RNA polymerase ...

*PSMD10

... a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 ... "The RTP site shared by the HIV-1 Tat protein and the 11S regulator subunit alpha is crucial for their effects on proteasome ... It also binds closely to the E3 ubiquitin ligase MDM2, which is a regulator of the degradation of p53 and retinoblastoma ... Accordingly, gene expression by degradation of transcription factors, such as p53, c-Jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, ...

*PSMC5

"The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative ... which interacts with the seven-membered alpha ring of 20S core particle and eastablishs an asymmetric interface between the 19S ... It also have subunits that can bind with nucleotides (e.g., ATPs) in order to facilitate the association between 19S and 20S ... These subunits can be categorized into two classes based on the ATP dependence of subunits, ATP-dependent subunits and ATP- ...

*EGLN1

In normoxia, HIF alpha subunits are marked for the ubiquitin-proteasome degradation pathway through hydroxylation of proline- ... "Structural basis for binding of hypoxia-inducible factor to the oxygen-sensing prolyl hydroxylases". Structure. 17 (7): 981-9. ... The catalytic domain consists of a double-stranded β-helix core that is stabilized by three α-helices packed along the major β- ... Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2), or prolyl hydroxylase domain-containing protein 2 (PHD2), is an enzyme ...

*EIF-W2 protein domain

"Multidomain organization of eukaryotic guanine nucleotide exchange translation initiation factor eIF-2B subunits revealed by ... The structure can be divided into a structural C-terminal core onto which the two N-terminal helices are attached. The core ... The W2 domain has a globular fold and is exclusively composed out of alpha-helices. ... the eIF-W2 domain functions as the binding site for Mnk eIF4E kinase, an enzyme that phosphorylates eukaryotic initiation ...

*TAF9

This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several ... The protein complex that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to ... "Induced alpha helix in the VP16 activation domain upon binding to a human TAF". Science. 277 (5330): 1310-3. doi:10.1126/ ... TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa, also known as TAF9, is a protein that in ...

*CUL4A

The smaller subunit of this Damaged DNA Binding protein complex is known as DDB2 and is able to directly bind DNA lesions ... Recent reports show that IMiDs bind to CRL4CRBN and promote the degradation of IKZN1 and IKZN3 transcription factors, which are ... RBX1 is a core component of Cullin-RING ubiquitin ligase (CRL) complexes and functions to recruit E2 ubiquitin conjugating ... CUL4A protein is 759 amino acids long and forms an extended, rigid structure primarily consisting of alpha-helices. At the N- ...

*RNA polymerase II

TATA-box binding protein), the subunit of the general transcription factor TFIID, than polymerase IIO form. The form polymerase ... RPB3 (POLR2C) - the third-largest subunit. Exists as a heterodimer with another polymerase subunit, POLR2J forming a core ... Alpha-Amanitin is a highly poisonous substance found in many mushrooms. The mushroom poison has different effects on the each ... In combination with several other polymerase subunits, the RPB1 subunit forms the DNA binding domain of the polymerase, a ...

*SMARCC2

Phelan ML, Sif S, Narlikar GJ, Kingston RE (1999). "Reconstitution of a core chromatin remodeling complex from SWI/SNF subunits ... "Architectural DNA binding by a high-mobility-group/kinesin-like subunit in mammalian SWI/SNF-related complexes". Proc. Natl. ... "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97-105. ... "A Human RNA Polymerase II Complex Containing Factors That Modify Chromatin Structure". Mol. Cell. Biol. 18 (9): 5355-63. PMC ...

*Tbf5 protein domain

... general transcription factor 2H subunit 5) is also known as the TTD group A (TTDA) subunit (and as Tfb5). The TTDA subunit is ... and part of a six-subunit complex of Rad3, Tfb1, Tfb2, Tfb4, Tfb5, and Ssl1 (referred to as core) In humans, the function of ... TTDA is present both bound to TFIIH, and as a free fraction that shuffles between the cytoplasm and nucleus; induction of NER- ... 2012). "Subunit architecture of general transcription factor TFIIH". Proc Natl Acad Sci U S A. 109 (6): 1949-54. doi:10.1073/ ...

*Alkali metal

... caused by the alpha decay of actinium-227.[35] Perey then attempted to determine the proportion of beta decay to alpha decay in ... Gottschlich, Michele M. (2001). The Science and Practice of Nutrition Support: A Case-based Core Curriculum. Kendall Hunt. p. ... The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... Sodium tetraphenylborate can also be classified as an organosodium compound since in the solid state sodium is bound to the ...

*Transcription factor II B

The first transcription factor to bind the DNA is TFIID, which binds via the TBP subunit to the TATA box. TFIIB then binds to ... Each of the domains has 5 alpha helices with a hydrophobic core. These two domains show a high sequence and structural ... The binding of TBP to DNA forms a 90° kink in the DNA and allows the TFIIB to clamp the TBP tightly to the DNA. The binding of ... Transcription factor II B (TFIIB) is a general transcription factor that is involved in the formation of the RNA polymerase II ...

core binding factor alpha
     Summary Report | CureHuntercore binding factor alpha Summary Report | CureHunter

... core binding factor plays a key role in several development pathways and in human disease; has been sequenced ... Transcription Factors: 20597*Core Binding Factors: 223*Core Binding Factor alpha Subunits: 18*core binding factor alpha: 16 ... core binding factor alpha. Subscribe to New Research on core binding factor alpha ... 11/07/2000 - "Binding of CBFalpha/AML/PEBP2alpha (core binding factor alpha/acute myelogenous leukemia/polyoma enhancer binding ...
more infohttp://www.curehunter.com/public/keywordSummaryC406688-core-binding-factor-alpha.do

Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript...Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript...

... alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2, (10ug), 10 µg. ... Home » cDNA » Mouse cDNA » Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 ( ... MC219266 Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), ... Properties for Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) ( ...
more infohttps://www.acris-antibodies.com/cdna/mouse-cdna/cbfa2t3-untagged-mouse-core-binding-factor-runt-domain-alpha-subunit-2-translocated-to-3-human-cbfa2t3-transcript-variant-2-10ug-mc219266.htm

REGULATOR: a database of metazoan transcription factors and maternal factors for developmental studiesREGULATOR: a database of metazoan transcription factors and maternal factors for developmental studies

Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (Predicted). ... PREDICTED: transcription initiation factor TFIID subunit 4-like[Cricetulus griseus].. 11. Mus musculus. 12396. Cbfa2t2; ... PREDICTED: transcription initiation factor TFIID subunit 4B-like[Cricetulus griseus].. 10. Cricetulus griseus. 100769929. ... PREDICTED: transcription initiation factor TFIID subunit 4-likeisoform 1 [Bombus impatiens].. 31. Pteropus vampyrus. ...
more infohttp://www.bioinformatics.org/regulator/page.php?act=list_t&acc=PF07531

CBFA2/RUNX1 translocation partner 2 ELISA Kits | Biocompare.comCBFA2/RUNX1 translocation partner 2 ELISA Kits | Biocompare.com

Compare CBFA2/RUNX1 translocation partner 2 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... Core Binding Factor Alpha Subunit 2 Translocated To 2 (. *. Detection Range: 0.313 ng/ml - 20 ng/ml ... Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target... read more ... CBFA2/RUNX1 translocation partner 2 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody- ...
more infohttps://www.biocompare.com/pfu/110627/soids/2-2265190/ELISA_Kit/ELISA_CBFA2RUNX1_translocation_partner_2

CBFA2T2 Gene - GeneCards | MTG8R Protein | MTG8R AntibodyCBFA2T2 Gene - GeneCards | MTG8R Protein | MTG8R Antibody

CBFA2/RUNX1 Translocation Partner 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... core binding factor Runt domain,alpha subunit 2,(RUNX1) translocated to 2,mainly expressed in brain,with several isoforms due ... Core-binding factor, runt domain, alpha subunit 2; translocated to, 2:. *Hs.153934 ... Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 2 2 3 ...
more infohttps://www.genecards.org/cgi-bin/carddisp.pl?gene=CBFA2T2

CBFA2T2 Gene - GeneCards | MTG8R Protein | MTG8R AntibodyCBFA2T2 Gene - GeneCards | MTG8R Protein | MTG8R Antibody

CBFA2/RUNX1 Partner Transcriptional Co-Repressor 2, including: function, proteins, disorders, pathways, orthologs, and ... core binding factor Runt domain,alpha subunit 2,(RUNX1) translocated to 2,mainly expressed in brain,with several isoforms due ... Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 2 2 3 ... Transcription Factor. Binding Sites. Gene Targets. GH20J033400. Promoter/Enhancer. 2.1. EPDnew Ensembl ENCODE CraniofacialAtlas ...
more infohttps://www.genecards.org/cgi-bin/carddisp.pl?gene=CBFA2T2&keywords=GH20J033476&prefilter=genomic_location

RNA-sequencing Analysis of Core Binding Factor AML Identifies Recurrent ZBTB7A Mutations and Defines RUNX1-CBFA2T3 Fusion...RNA-sequencing Analysis of Core Binding Factor AML Identifies Recurrent ZBTB7A Mutations and Defines RUNX1-CBFA2T3 Fusion...

RNA-sequencing Analysis of Core Binding Factor AML Identifies Recurrent ZBTB7A Mutations and Defines RUNX1-CBFA2T3 Fusion ... Core Binding Factor Alpha 2 Subunit / genetics* Actions. * Search in PubMed * Search in MeSH ... RNA-sequencing Analysis of Core Binding Factor AML Identifies Recurrent ZBTB7A Mutations and Defines RUNX1-CBFA2T3 Fusion ... RNA-sequencing Analysis of Core Binding Factor AML Identifies Recurrent ZBTB7A Mutations and Defines RUNX1-CBFA2T3 Fusion ...
more infohttps://pubmed.ncbi.nlm.nih.gov/26968532/

Runt-Related Transcription Factor 1 (RUNX1) Promotes TGF-β-Induced Renal Tubular Epithelial-to-Mesenchymal Transition (EMT) and...Runt-Related Transcription Factor 1 (RUNX1) Promotes TGF-β-Induced Renal Tubular Epithelial-to-Mesenchymal Transition (EMT) and...

Runt-related transcription factor 1(RUNX1) plays a vital role in hematopoiesis via Endothelial-to-Hematop … ... Core Binding Factor Alpha 2 Subunit / genetics Actions. * Search in PubMed * Search in MeSH ... and Renal Fibrosis Through the PI3K Subunit p110δ Tong Zhou 1 , Maocai Luo 2 , Wei Cai 3 , Siyuan Zhou 2 , Danying Feng 2 , ... Tong Zhou 1 , Maocai Luo 2 , Wei Cai 3 , Siyuan Zhou 2 , Danying Feng 2 , Chundi Xu 4 , Hongyan Wang 5 ...
more infohttps://pubmed.ncbi.nlm.nih.gov/29759484/

CBFA2T3 - PrimePCR Assay and Template | Life Science | Bio-RadCBFA2T3 - PrimePCR Assay and Template | Life Science | Bio-Rad

Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Assay Type: Probe Assay Design: Exonic Application: Gene ... Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Assay Type: SYBR® Green Assay Design: Exonic Application ... Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting ... R2. ,0.99. These DNA primer pairs were designed by prioritizing the gene regions most commonly found in transcript variants. ...
more infohttp://www.bio-rad.com/en-us/prime-pcr-assays/gene/cbfa2t3-rabbit

CBFA2T3 (Human) Recombinant Protein (P01) - (H00000863-P01) - Products - AbnovaCBFA2T3 (Human) Recombinant Protein (P01) - (H00000863-P01) - Products - Abnova

core-binding factor, runt domain, alpha subunit 2; translocated to, 3. *Omim ID: ... and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi ... MTG8-related gene 2,myeloid translocation gene on chromosome 16,zinc finger MYND domain-containing protein 4 ...
more infohttp://www.abnova.com/products/products_detail.asp?catalog_id=H00000863-P01

anti-MTGR1 antibody  | GeneTexanti-MTGR1 antibody | GeneTex

... alpha subunit 2; translocated to, 2) for WB. Anti-MTGR1 pAb (GTX16137) is tested in Human samples. 100% Ab-Assurance. ... core-binding factor, runt domain, alpha subunit 2; translocated to, 2. Background. Myeloid tumor related gene (MTGR1) is found ... For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple ...
more infohttp://www.genetex.com/MTGR1-antibody-GTX16137.html

CBFA2T2 - WikipediaCBFA2T2 - Wikipedia

"Entrez Gene: CBFA2T2 core-binding factor, runt domain, alpha subunit 2; translocated to, 2". Rual JF, Venkatesan K, Hao T, ... The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several ... 18 (2): 846-58. PMC 108796 . PMID 9447981. Fracchiolla NS, Colombo G, Finelli P, Maiolo AT, Neri A (1998). "EHT, a new member ... 241 (2): 287-95. doi:10.1016/S0378-1119(99)00481-3. PMID 10675041. Hoogeveen AT, Rossetti S, Stoyanova V, Schonkeren J, ...
more infohttps://en.wikipedia.org/wiki/CBFA2T2

CBFA2T3 - WikipediaCBFA2T3 - Wikipedia

"Entrez Gene: CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3". Hoogeveen AT, Rossetti S, ... and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi ... makes distinct contacts with multiple histone deacetylases and binds mSin3A through its oligomerization domain". Mol. Cell. ... 18 (2): 846-58. PMC 108796 . PMID 9447981. Gamou T, Kitamura E, Hosoda F, Shimizu K, Shinohara K, Hayashi Y, Nagase T, Yokoyama ...
more infohttps://en.wikipedia.org/wiki/CBFA2T3

Sequence DetailSequence Detail

Core-binding factor subunit alpha-1; Short=CBF-alpha-1;AltName: Full=Oncogene AML-3;AltName: Full=Osteoblast-specific ... the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. Virology. 1993 May;194(1):314-31 ... J:19082 Ogawa E, et al., PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila ... J:49076 Xiao ZS, et al., Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor. ...
more infohttp://www.informatics.jax.org/sequence/Q08775

Cbfa2t3 - PrimePCR Assay and Template | Life Science | Bio-RadCbfa2t3 - PrimePCR Assay and Template | Life Science | Bio-Rad

Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: SYBR® Green Assay Design: exonic ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Assay Design: exonic ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Application: Gene Expression ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Application: Gene Expression ...
more infohttp://www.bio-rad.com/en-us/prime-pcr-assays/gene/cbfa2t3-mouse

Cbfa2t2 monoclonal antibody, clone KT42 - (MAB1674) - Products - AbnovaCbfa2t2 monoclonal antibody, clone KT42 - (MAB1674) - Products - Abnova

core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (human) ... CBFA2T2 identified gene homolog,OTTMUSP00000016821,core-binding factor runt domain alpha subunit 2 translocated to 2 homolog, ... core-binding factor, runt domain, alpha subunit 2, translocated to, 2 homolog ... Novel binding partners of Ldb1 are required for haematopoietic development.. Meier N, Krpic S, Rodriguez P, Strouboulis J, ...
more infohttp://www.abnova.com/products/products_detail.asp?catalog_id=MAB1674

Protein-Protein Interaction - TFCatWikiProtein-Protein Interaction - TFCatWiki

core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human). ... ccr4-not transcription complex, subunit 3. Cops2 12848. cop9 (constitutive photomorphogenic) homolog, subunit 2 (arabidopsis ... cbp/p300-interacting transactivator, with glu/asp-rich carboxy-terminal domain, 2. ...
more infohttp://cisreg.ca/TFCatWiki/index.php?title=Protein-Protein_Interaction&diff=prev&oldid=15623&printable=yes

TCF3 | Cancer Genetics WebTCF3 | Cancer Genetics Web

Core Binding Factor Alpha 2 Subunit. *MicroRNAs. *DNA-Binding Proteins. *FISH. *TCF3 ... bHLH transcription factor binding - cell development - chromatin binding - cytoplasm - DNA binding - E-box binding - G1 phase ... transcription factor binding - transcription factor complex - transcription regulatory region DNA binding - transcription, DNA- ... sequence-specific DNA binding - sequence-specific DNA binding transcription factor activity - T cell differentiation in thymus ...
more infohttp://www.cancerindex.org/geneweb/TCF3.htm

CBFA2T3 Antibody 17190-1-AP  | ProteintechCBFA2T3 Antibody 17190-1-AP | Proteintech

core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Calculated molecular weight: ... CBFA2T3, ETO2, hMTG16, MTG16, MTG8 related protein 2, MTGR2, Protein CBFA2T3, ZMYND4 ...
more infohttps://www.ptglab.com/Products/CBFA2T3-Antibody-17190-1-AP.htm

ZFIN Publication: Kalev-Zylinska et al., 2002ZFIN Publication: Kalev-Zylinska et al., 2002

Acute Disease; Amino Acid Sequence; Animals; Base Sequence; Cell Differentiation; Core Binding Factor Alpha 2 Subunit; DNA, ... Complementary; DNA-Binding Proteins/classification; DNA-Binding Proteins/genetics; DNA-Binding Proteins/physiology*; Disease ... Transcription Factors/classification; Transcription Factors/genetics; Transcription Factors/physiology*; Transgenes; Zebrafish/ ... runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl, and expression of ...
more infohttp://zfin.org/ZDB-PUB-020410-3

ZFIN Publication: Li et al., 2015ZFIN Publication: Li et al., 2015

Core Binding Factor Alpha 2 Subunit/metabolism; Female; Gene Expression Regulation; Hematopoiesis*; Hematopoietic Stem Cell ... Transcription Factor AP-1/metabolism; Transcription, Genetic; Zebrafish/embryology* ...
more infohttp://zfin.org/ZDB-PUB-150725-11

RUNX2 elisa kit | Mouse runt-related transcription factor 2 ELISA Kit-NP 001139510.1RUNX2 elisa kit | Mouse runt-related transcription factor 2 ELISA Kit-NP 001139510.1

Mouse runt-related transcription factor 2 ELISA Kit-NP_001139510.1 (MBS934869) product datasheet at MyBioSource, ELISA Kits ... CBF-alpha-1; PEA2-alpha A; PEBP2 alpha A; AKV core binding factor; core binding factor alpha 1; runt domain, alpha subunit 1; ... PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit. ... CBF-alpha 1; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A; runt-related transcription factor 2 ...
more infohttps://www.mybiosource.com/prods/ELISA-Kit/Mouse/runt-related-transcription-factor-2/RUNX2/datasheet.php?products_id=934869
  • Molecular, cellular, genetic and biochemical approaches have identified distinct protein segments, termed intranuclear-targeting signals, that are responsible for directing regulatory factors to specific subnuclear sites. (umassmed.edu)
  • Thus, highly enriched GFP-positive AGM HSCs will serve as a basis for the future examination of the cellular and molecular factors involved in the induction and expansion of adult HSCs. (ox.ac.uk)
  • Kageyama R, Sasai Y, Nakanishi S. Molecular characterization of transcription factors that bind to the cAMP responsive region of the substance P precursor gene. (labome.org)
  • Sigma factors are distinguished by their characteristic molecular weights. (wikipedia.org)
  • For example, σ 70 is the sigma factor with a molecular weight of 70 kDa . (wikipedia.org)
  • To understand the molecular mechanisms for synergy between these transcription factors in the context of chromatin, we used in vivo footprinting to study the requirements for protein binding to Edelta within wild-type and mutant versions of a human TCR delta minilocus in stably transfected Jurkat cells. (duke.edu)
  • Molecular recognition is integral to biological function and frequently involves preferred binding of a molecule to one of several exchanging ligand conformations in solution. (weizmann.ac.il)
  • In an effort to characterize the interactions further we report here the results of an NMR-based titration study of hTRF1 and DnaK, where both molecular components are monitored simultaneously, leading to a binding model. (weizmann.ac.il)
  • In the large subunit, about 1/3 of the 23S rRNA nucleotides are at least in van der Waal's contact with protein, and L22 interacts with all six domains of the 23S rRNA. (embl.de)
  • Parathyroid Hormone-responsive Smad3-related Factor, Tmem119, Promotes Osteoblast Differentiation and Interacts with the Bone Morphogenetic Protein-Runx2 Pathway. (jax.org)
  • Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. (abcam.com)
  • The Runx1 transcription factor inhibits the differentiation of naive CD4+ T cells into the Th2 lineage by repressing GATA3 expression. (nih.gov)
  • Differentiation of naive CD4+ T cells into helper T (Th) cells is controlled by a combination of several transcriptional factors. (nih.gov)
  • Human serum response factor (SRF) is a ubiquitous nuclear protein important for cell proliferation and differentiation. (ebi.ac.uk)
  • Here we show that the mouse transcription factor Runx1, a key regulator of myeloid cell proliferation and differentiation, is expressed in forebrain amoeboid microglia during the first two postnatal weeks. (unboundmedicine.com)
  • 4. A host cell comprising a recombinant nucleic acid molecule according to claim 2. (google.es)
  • CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. (stjohnslabs.com)
  • These transcription factors have specific activator or repressor sequences of corresponding nucleotides that attach to specific promoters and regulate gene expression. (wikipedia.org)
  • Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. (proteopedia.org)
  • Preexisting long‐range interactions between HIF ‐binding sites and promoters as well as other enhancers shape the transcriptional architecture of the cellular response to hypoxia. (embopress.org)
  • Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. (mybiosource.com)
  • The codons of the mRNA are exposed on the ribosome to allow tRNA binding. (embl.de)
  • About 2/3 of the mass of the ribosome consists of RNA and 1/3 of protein. (embl.de)
  • It permits the direct binding of the 40S ribosome subunit in the absence of additional translation factors ( 35 ). (asm.org)
  • This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. (proteopedia.org)
  • In such a process the bound structure can be selected from the ensemble of interconverting ligands a priori (conformational selection, CS) or may form once the ligand is bound (induced fit, IF). (weizmann.ac.il)
  • Under nonstressed conditions, NRF2, a member of the cap"n"collar family of basic leucine zipper transcription factors, is repressed in the cytosol by Kelch-like ECH-associated protein 1 (KEAP1) [ 3 - 6 ]. (hindawi.com)
  • It binds in the cytoplasm the human BAF protein which prevent autointegration of the viral genome, and might be included in virions at the ration of zero to 3 BAF dimer per virion. (proteopedia.org)
  • We previously established mouse lines that express a DNA binding domain of Runx1 from a transgene in a T-lineage-specific way (24). (nih.gov)
  • A core domain of around 90 amino acids is sufficient for the activities of DNA-binding, dimerisation and interaction with accessory factors. (ebi.ac.uk)
  • Among the exons are two defined domains, namely the runt homology domain (RHD) or the runt domain (exons 2, 3 and 4), and the transactivation domain (TAD) (exon 6). (hitchhikersgui.de)
  • 1998). Akt activation by growth factors is a multiple-step process: the role of the PH domain. (core.ac.uk)
  • They aim to define the targets of every vertebrate DNA-binding domain - and it's not as crazy as it sounds. (wordpress.com)
  • These modifications in the intranuclear targeting of transcription factors might abrogate fidelity of gene expression in tumor cells by influencing the spatial organization and/or assembly of machineries involved in the synthesis and processing of gene transcripts. (umassmed.edu)
  • The gene expression profiles of UT-7/Epo and UT-7/EN cells were compared using HumanHT-12 v4 BeadChip array and by validation with qRT-PCR. (tamuc.edu)
  • 0.01), whereas β-catenin gene expression remained non-significant at 24, 48, and 72 hours relative to un-supplemented UT-7/EN cells. (tamuc.edu)
  • runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl, and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche. (zfin.org)
  • The intraluminal hematopoietic clusters along these vessels, together with the role of the Runx1 transcription factor in cluster and HSC formation and the HSC/endothelial/mesenchymal Runxl expression pattern, strongly suggest a vascular endothelial/mesenchymal origin for the first HSCs. (ox.ac.uk)
  • 8) In general, the cytogenetic landscape of AML is characterized either by balanced chromosomal rearrangements, often involving hematopoietic transcription factors (TFs), or by chromosomal losses and gains similar to those present in MDS. (thefreelibrary.com)
  • Pan‐genomic analyses, using chromatin immunoprecipitation and transcript profiling, have revealed large numbers of HIF ‐binding sites that are generally associated with hypoxia‐inducible transcripts, even over long chromosomal distances. (embopress.org)
  • However, these studies do not define the specific targets of HIF ‐binding sites and do not reveal how induction of HIF affects chromatin conformation over distantly connected functional elements. (embopress.org)
  • The name of this superfamily has been modified since the most recent official CATH+ release (v4_2_0). (cathdb.info)
  • We also identified a plethora of transporters belonging to the ABC and major facilitator superfamily along with known MDR transcription factors which explained its high tolerance to antifungal drugs. (biomedcentral.com)
  • translocated to, 3 (CBFA2T3), transcript variant 2 with C terminal Flag and His tag. (trademetro.net)
  • Electromobility Shift Assay Reveals Evidence in Favor of Allele-Specific Binding of RUNX1 to the 5' Hypersensitive Site 4-Locus Control Region. (cdc.gov)
  • Akasaki K, Michihara A, Fujiwara Y, Mibuka K, Tsuji H. Biosynthetic transport of a major lysosome-associated membrane glycoprotein 2, lamp-2: a significant fraction of newly synthesized lamp-2 is delivered to lysosomes by way of early endosomes. (labome.org)
  • Their activity is, unfortunately, hampered by the ATP-binding cassette (ABC) efflux transporter, P-glycoprotein. (thefreelibrary.com)