Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.
A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
Myosin type II isoforms found in smooth muscle.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.
Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.
A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.
The process of bone formation. Histogenesis of bone including ossification.
Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.
Established cell cultures that have the potential to propagate indefinitely.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.
Factors that form a preinitiation complex at promoters that are specifically transcribed by RNA POLYMERASE I.
An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.
One of a pair of excretory organs (mesonephroi) which grows caudally to the first pair (PRONEPHROI) during development. Mesonephroi are the permanent kidneys in adult amphibians and fish. In higher vertebrates, proneprhoi and most of mesonephroi degenerate with the appearance of metanephroi. The remaining ducts become WOLFFIAN DUCTS.
Progenitor cells from which all blood cells derive.
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
The gamete-producing glands, OVARY or TESTIS.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
The processes whereby the internal environment of an organism tends to remain balanced and stable.
The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).
A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.
A progressive, malignant disease of the blood-forming organs, characterized by distorted proliferation and development of leukocytes and their precursors in the blood and bone marrow. Leukemias were originally termed acute or chronic based on life expectancy but now are classified according to cellular maturity. Acute leukemias consist of predominately immature cells; chronic leukemias are composed of more mature cells. (From The Merck Manual, 2006)
A mutation in which a codon is mutated to one directing the incorporation of a different amino acid. This substitution may result in an inactive or unstable product. (From A Dictionary of Genetics, King & Stansfield, 5th ed)
Any detectable and heritable alteration in the lineage of germ cells. Mutations in these cells (i.e., "generative" cells ancestral to the gametes) are transmitted to progeny while those in somatic cells are not.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Immunoglobulins produced in a response to BACTERIAL ANTIGENS.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.

Three distinct domains in TEL-AML1 are required for transcriptional repression of the IL-3 promoter. (1/859)

A cytogenetically cryptic (12;21) translocation is the most common molecular abnormality identified in childhood acute lymphoblastic leukemia (ALL), and it generates a chimeric TEL-AML1 protein. Fusion of the Helix-Loop-Helix (HLH) (also called the pointed) domain of TEL to AML1 has been suggested to convert AML1 from a transcriptional activator to a repressor. To define the structural features of this chimeric protein required for repression, we analysed the transcriptional activity of a series of TEL-AML1 mutants on the AML1-responsive interleukin-3 (IL-3) promoter, a potentially relevant gene target. Our results demonstrate that TEL-AML1 represses basal IL-3 promoter activity in lymphoid cells, and deletion mutant analysis identified three distinct domains of TEL-AML1 that are required for repression; the HLH (pointed) motif contained in the TEL portion of TEL-AML1, and both the runt homology domain (Rhd) and the 74 amino acids downstream of the Rhd that are present in the AML1 portion of the fusion protein. Although AML1B (and a shorter AML1 isoform, AML1A) have transcriptional activating activity on the IL-3 promoter, fusion of the AML1 gene to the TEL gene generates a repressor of IL-3 expression. Consistent with this activity, freshly isolated human ALL cells that contain TEL-AML1 do not express IL-3.  (+info)

Biallelic and heterozygous point mutations in the runt domain of the AML1/PEBP2alphaB gene associated with myeloblastic leukemias. (2/859)

The AML1 gene encoding the DNA-binding alpha-subunit in the Runt domain family of heterodimeric transcription factors has been noted for its frequent involvement in chromosomal translocations associated with leukemia. Using reverse transcriptase-polymerase chain reaction (RT-PCR) combined with nonisotopic RNase cleavage assay (NIRCA), we found point mutations of the AML1 gene in 8 of 160 leukemia patients: silent mutations, heterozygous missense mutations, and biallelic nonsense or frameshift mutations in 2, 4, and 2 cases, respectively. The mutations were all clustered within the Runt domain. Missense mutations identified in 3 patients showed neither DNA binding nor transactivation, although being active in heterodimerization. These defective missense mutants may be relevant to the predisposition or progression of leukemia. On the other hand, the biallelic nonsense mutants encoding truncated AML1 proteins lost almost all functions examined and may play a role in leukemogenesis leading to acute myeloblastic leukemia.  (+info)

Mutual activation of Ets-1 and AML1 DNA binding by direct interaction of their autoinhibitory domains. (3/859)

The transcription factors Ets-1 and AML1 (the alphaBl subunit of PEBP2/CBF) play critical roles in hematopoiesis and leukemogenesis, and cooperate in the transactivation of the T cell receptor (TCR) beta chain enhancer. The DNA binding capacity of both factors is blocked intramolecularly but can be activated by the removal of negative regulatory domains. These include the exon VII domain for Ets-1 and the negative regulatory domain for DNA binding (NRDB) for alphaB1. Here we report that the direct interaction between the two factors leads to a reciprocal stimulation of their DNA binding activity and activation of their transactivation function. Detailed mapping revealed two independent contact points involving the exon VII and NRDB regions as well as the two DNA binding domains. Using deletion variants and dominant interfering mutants, we demonstrate that the interaction between exon VII and NRDB is necessary and sufficient for cooperative DNA binding. The exon VII and NRDB motifs are highly conserved in evolution yet deleted in natural variants, suggesting that the mechanism described is of biological relevance. The mutual activation of DNA binding of Ets and AML1 through the intermolecular interaction of autoinhibitory domains may represent a novel principle for the regulation of transcription factor function.  (+info)

A novel ubiquitin-specific protease, UBP43, cloned from leukemia fusion protein AML1-ETO-expressing mice, functions in hematopoietic cell differentiation. (4/859)

Using PCR-coupled subtractive screening-representational difference analysis, we have cloned a novel gene from AML1-ETO knockin mice. This gene is highly expressed in the yolk sac and fetal liver of the knockin mice. Nucleotide sequence analysis indicates that its cDNA contains an 1,107-bp open reading frame encoding a 368-amino-acid polypeptide. Further protein sequence and protein translation analysis shows that it belongs to a family of ubiquitin-specific proteases (UBP), and its molecular mass is 43 kDa. Therefore, we have named this gene UBP43. Like other ubiquitin proteases, the UBP43 protein has deubiquitinating enzyme activity. Protein ubiquitination has been implicated in many important cellular events. In wild-type adult mice, UBP43 is highly expressed in the thymus and in peritoneal macrophages. Among nine different murine hematopoietic cell lines analyzed, UBP43 expression is detectable only in cell lines related to the monocytic lineage. Furthermore, its expression is regulated during cytokine-induced monocytic cell differentiation. We have investigated its function in the hematopoietic myeloid cell line M1. UBP43 was introduced into M1 cells by retroviral gene transfer, and several high-expressing UBP43 clones were obtained for further study. Morphologic and cell surface marker examination of UBP43/M1 cells reveals that overexpression of UBP43 blocks cytokine-induced terminal differentiation of monocytic cells. These data suggest that UBP43 plays an important role in hematopoiesis by modulating either the ubiquitin-dependent proteolytic pathway or the ubiquitination state of another regulatory factor(s) during myeloid cell differentiation.  (+info)

Regulation of c-fos gene transcription and myeloid cell differentiation by acute myeloid leukemia 1 and acute myeloid leukemia-MTG8, a chimeric leukemogenic derivative of acute myeloid leukemia 1. (5/859)

Both acute myeloid leukemia 1 and c-Fos are regulatory factors of hematopoietic cell differentiation. We identified that the c-fos promoter contains an acute myeloid leukemia 1 binding site at nucleotide positions -6-+14. c-fos promoter activity was induced by transient overexpression of acute myeloid leukemia 1 in Jurkat T-cells, but not by that of the short form of acute myeloid leukemia 1-MTG8, a chimeric acute myeloid leukemia 1 protein. In 32Dcl3 myeloid cells, stable overexpression of acute myeloid leukemia 1-MTG8 blocked the c-fos gene transcription and cell differentiation, but that of acute myeloid leukemia did not. These data suggest that acute myeloid leukemia 1 and acute myeloid leukemia 1-MTG8 reciprocally regulate the myeloid cell differentiation, possibly by the way of regulating c-fos gene transcription.  (+info)

Solution properties of the free and DNA-bound Runt domain of AML1. (6/859)

The Runt domain is responsible for specific DNA and protein-protein interactions in a family of transcription factors which includes human AML1. Structural data on the Runt domain has not yet become available, possibly due to solubility and stability problems with expressed protein fragments. Here we describe the optimization and characterization of a 140-residue fragment, containing the Runt domain of AML1, which is suitable for structural studies. The fragment of AML1 including amino acids 46-185 [AML1 Dm(46-185)] contains a double cysteine-->serine mutation which does not affect Runt domain structure or DNA-binding affinity. Purified AML1 Dm(46-185) is soluble and optimally stable in a buffer containing 200 mm MgSO4 and 20 mm sodium phosphate at pH 6.0. Nuclear magnetic resonance and circular dichroism spectroscopy indicate that the Runt domain contains beta-sheet, but little or no alpha-helical secondary structure elements. The 45 N-terminal residues of AML1 are unstructured and removal of the N-terminal enhances sequence-specific DNA binding. The NMR spectrum of AML1 Dm(46-185) displays a favorable chemical shift dispersion and resolved NOE connectivities are readily identified, suggesting that a structure determination of this Runt domain fragment is feasible. A titration of 15N-labelled AML1 Dm(46-185) with a 14-bp cognate DNA duplex results in changes in the 15N NMR heteronuclear single quantum coherence spectrum which indicate the formation of a specific complex and structural changes in the Runt domain upon DNA binding.  (+info)

Induction of apoptosis in myeloid leukaemic cells by ribozymes targeted against AML1/MTG8. (7/859)

The translocation (8;21)(q22;q22) is a karyotypic abnormality detected in acute myeloid leukaemia (AML) M2 and results in the formation of the chimeric fusion gene AML1/MTG8. We previously reported that two hammerhead ribozymes against AML1/MTG8 cleave this fusion transcript and also inhibit the proliferation of myeloid leukaemia cell line Kasumi-1 which possesses t(8;21)(q22;q22). In this study, we investigated the mechanisms of inhibition of proliferation in myeloid leukaemic cells with t(8;21)(q22;q22) by ribozymes. These ribozymes specifically inhibited the growth of Kasumi-1 cells, but did not affect the leukaemic cells without t(8;21)(q22;q22). We observed the morphological changes including chromatin condensation, fragmentation and the formation of apoptotic bodies in Kasumi-1 cells incubated with ribozymes for 7 days. In addition, DNA ladder formation was also detected after incubation with ribozymes which suggested the induction of apoptosis in Kasumi-1 cells by the AML1/MTG8 ribozymes. However, the ribozymes did not induce the expression of CD11b and CD14 antigens in Kasumi-1 cells. The above data suggest that these ribozymes therefore inhibit the growth of myeloid leukaemic cells with t(8;21)(q22;q22) by the induction of apoptosis, but not differentiation. We conclude therefore that the ribozymes targeted against AML1/MTG8 may have therapeutic potential for patients with AML carrying t(8;21)(q22;q22) while, in addition, the product of the chimeric gene is responsible for the pathogenesis of myeloid leukaemia.  (+info)

Expression of AML1-d, a short human AML1 isoform, in embryonic stem cells suppresses in vivo tumor growth and differentiation. (8/859)

The human AML1 gene encodes a heterodimeric transcription factor which plays an important role in mammalian hematopoiesis. Several alternatively spliced AML1 mRNA species were identified, some of which encode short protein products that lack the transactivation domain. When transfected into cells these short isoforms dominantly suppress transactivation mediated by the full length AML1 protein. However, their biological function remains obscure. To investigate the role of these short species in cell proliferation and differentiation we generated embryonic stem (ES) cells overexpressing one of the short isoforms, AML1-d, as well as cells expressing the full length isoforms AML1-b and AML2. The in vitro growth rate and differentiation of the transfected ES cells were unchanged. However, overexpression of AML1-d significantly affected the ES cells' ability to form teratocarcinomas in vivo in syngeneic mice, while a similar overexpression of AML1-b and AML2 had no effect on tumor formation. Histological analysis revealed that the AML1-d derived tumors were poorly differentiated and contained numerous apoptotic cells. These data highlight the pleiotropic effects of AML1 gene products and demonstrate for the first time an in vivo growth regulation function for the short isoform AML1-d.  (+info)

TY - JOUR. T1 - Prognostic value of AML 1/ETO fusion transcripts in patients with acute myelogenous leukemia.. AU - Cho, Eun Kyung. AU - Bang, Soo Mee. AU - Ahn, Jeong Yeal. AU - Yoo, Seung Min. AU - Park, Pil Whan. AU - Seo, Yieh Hea. AU - Shin, Dong Bok. AU - Lee, Jae Hoon. PY - 2003/1/1. Y1 - 2003/1/1. N2 - BACKGROUND: The t (8;21) (q22;q22), which produces the fusion gene AML1/ETO, is associated with relatively good prognosis and, in particular, with a good response to cytosine arabinoside. Analysis of t (8;21) positive leukemic blasts has shown characteristic morphological and immunological features. We performed this study to investigate the incidence of AML1/ETO rearrangement in adult acute myelogenous leukemia (AML), especially in M2 subtype, to make a comparison of clinical, morphological and immunophenotypic characteristics between AML1/ETO rearrangement positive and negative group in patients with AML and to analyze the correlation with other biological parameters. METHODS: From May ...
The t(8;21) acute myeloid leukemia (AML)-associated oncoprotein AML1-ETO disrupts normal hematopoietic differentiation. Here, we have investigated its effects on the transcriptome and epigenome in t(8,21) patient cells. AML1-ETO binding was found at promoter regions of active genes with high levels of histone acetylation but also at distal elements characterized by low acetylation levels and binding of the hematopoietic transcription factors LYL1 and LMO2. In contrast, ERG, FLI1, TAL1, and RUNX1 bind at all AML1-ETO-occupied regulatory regions, including those of the AML1-ETO gene itself, suggesting their involvement in regulating AML1-ETO expression levels. While expression of AML1-ETO in myeloid differentiated induced pluripotent stem cells (iPSCs) induces leukemic characteristics, overexpression increases cell death. We find that expression of wild-type transcription factors RUNX1 and ERG in AML is required to prevent this oncogene overexpression. Together our results show that the interplay ...
KIT gene mutations occur in approximately 25% of cases of acute myeloid leukemia (AML) with t(8;21) or inv(16), also referred to as core-binding factor AML. These mutations are predominately small length affecting mutations in KIT exon 8 and substitution mutations in KIT exon 17. KIT mutations in core-binding factor AML may be an adverse prognostic factor in this otherwise favorable-risk disease. This DNA sequencing test detects mutations in exons 8 and 17 of the KIT gene in blood and bone marrow specimens. This test will detect mutations in specimens when at least 30% of the nucleated cells carry the mutation.. ...
2018 The Author(s) Oncogenic transcription factors such as the leukemic fusion protein RUNX1/ETO, which drives t(8;21) acute myeloid leukemia (AML), constitute cancer-specific but highly challenging therapeutic targets. We used epigenomic profiling data for an RNAi screen to interrogate the transcriptional network maintaining t(8;21) AML. This strategy identified Cyclin D2 (CCND2) as a crucial transmitter of RUNX1/ETO-driven leukemic propagation. RUNX1/ETO cooperates with AP-1 to drive CCND2 expression. Knockdown or pharmacological inhibition of CCND2 by an approved drug significantly impairs leukemic expansion of patient-derived AML cells and engraftment in immunodeficient murine hosts. Our data demonstrate that RUNX1/ETO maintains leukemia by promoting cell cycle progression and identifies G1 CCND-CDK complexes as promising therapeutic targets for treatment of RUNX1/ETO-driven AML. Using in vitro and in vivo screens to identify essential RUNX1/ETO transcriptional targets in AML, Martinez-Soria ...
Buy our Recombinant Human RUNX2 protein. Ab112259 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE. Abcam provides…
Loss of RUNX1/ETO Triggers C/EBPα-Driven Reorganization of the Leukemic Transcriptional Network(A) RUNX1/ETO and CEBPA mRNA expression levels in Kasumi-1 cells
CATAGAGCCA GCGGGCGCGG GCGGGACGGG CGCCCCGCGG CCGGACCCAG CCAGGGCACC ACGCTGCCCG GCCCTGCGCC GCCAGGCACT TCTTTCCGGG ^1 ^11 ^21 ^31 ^41 ^51 ^61 ^71 ^81 ^91 GCTCCTAGGG ACGCCAGAAG GAAGTCAACC TCTGCTGCTT CTCCTTGGCC TGCGTTGGAC CTTCCTTTTT TTGTTGTTTT TTTTTGTTTT TCCCCTTTCT ^101 ^111 ^121 ^131 ^141 ^151 ^161 ^171 ^181 ^191 TCCTTTTGAA TTAACTGGCT TCTTGGCTGG ATGTTTTCAA CTTCTTTCCT GGCTGCGAAC TTTTCCCCAA TTGTTTTCCT TTTACAACAG GGGGAGAAAG ^201 ^211 ^221 ^231 ^241 ^251 ^261 ^271 ^281 ^291 TGCTCTGTGG TCCGAGGCGA GCCGTGAAGT TGCGTGTGCG TGGCAGTGTG CGTGGCAGGA TGTGCGTGCG TGTGTAACCC GAGCCGCCCG ATCTGTTTCG ^301 ^311 ^321 ^331 ^341 ^351 ^361 ^371 ^381 ^391 ATCTGCGCCG CGGAGCCCTC CCTCAAGGCC CGCTCCACCT GCTGCGGTTA CGCGGCGCTC GTGGGTGTTC GTGCCTCGGA GCAGCTAACC GGCGGGTGCT ^401 ^411 ^421 ^431 ^441 ^451 ^461 ^471 ^481 ^491 GGGCGACGGT GGAGGAGTAT CGTCTCGCTG CTGCCCGAGT CAGGGCTGAG TCACCCAGCT GATGTAGACA GTGGCTGCCT TCCGAAGAGT GCGTGTTTGC ^501 ^511 ^521 ^531 ^541 ^551 ^561 ^571 ^581 ^591 ATGTGTGTGA CTCTGCGGCT GCTCAACTCC CAACAAACCA GAGGACCAGC ...
Chromosome changes in the bone marrow (BM) of patients with persistent cytopenia are often considered diagnostic for a myelodysplastic syndrome (MDS). Comprehensive cytogenetic evaluations may give evidence of the real pathogenetic role of these changes in cases with cytopenia without morphological signs of MDS. Chromosome anomalies were found in the BM of three patients, without any morphological evidence of MDS: 1) an acquired complex rearrangement of chromosome 21 in a boy with severe aplastic anaemia (SAA); the rearrangement caused the loss of exons 2-8 of the RUNX1 gene with subsequent hypoexpression. 2) a constitutional complex rearrangement of chromosome 21 in a girl with congenital thrombocytopenia; the rearrangement led to RUNX1 disruption and hypoexpression. 3) an acquired paracentric inversion of chromosome 1, in which two regions at the breakpoints were shown to be lost, in a boy with aplastic anaemia; the MPL gene, localized in chromosome 1 short arms was not mutated neither disrupted, but
The TEL-AML1 fusion not only characterizes the most frequent genetic rearrangement in initial childhood ALL (20-25%) but its presence has also been associated with a favorable prognosis (9, 10, 11 , 28 , 32) . In clinical studies on initial ALL, probability of event-free survival (EFS) at 4 years was as high as 90-100% for TEL-AML1+ patients (9 , 10 , 32) . These results certainly do not represent final outcome considering that, regardless of the different prevalence of TEL-AML1 positivity at relapse of BCP-ALL (range, 3- 28%), TEL-AML1+ leukemia is biologically characterized by a long duration of first CR and that the majority of relapses (80%) occur off-therapy (median, 46 months; range, 13-125 months; Refs. 18, 19, 20, 21, 22, 23 , 33 ). The prevalence of TEL-AML1 positivity in our ongoing prospective study on first relapse of BCP-ALL is ∼17% (31 of 178 children; 33 ).. Obviously, the predictive value of TEL-AML1 positivity alone is insufficient to stratify patients to appropriate treatment ...
Further we asked if VLA-4 and VLA-5 integrin upregulation is maintained by RUNX1/ETO in the transformed human leukemia cell line Kasumi-1, derived from a t(8;21)+ AML patient. Kasumi-1 cells, which express RUNX1/ETO and to a lesser extent RUNX1/ETOtr,4 bear high levels of VLA-4 whereas the integrin αL subunit is absent in these cells. We specifically down-regulated RUNX1/ETO via lentivirally delivered shRNA targeting the RUNX1/ETO breakpoint sequences (shRE), which are present in both full length and truncated forms (Online Supplementary Figure S3). At Day 4 after transduction with vectors co-expressing shRE and eGFP, α4, α5 and β1 expression levels were significantly reduced as assessed using flow cytometry, while CXCR4 levels remained unaltered (Figure 1I). Similar results were obtained with NHR2 competitive peptides (N89) (Figure 1J), which also interfere with both RUNX1/ETO forms by disrupting RUNX1/ETO tetramer formation.6 These results suggest that integrin subunit expression remains ...
MGA is an incompletely studied gene with a high mutation frequency in MLL-PTD AML (9%) and in core bind factor AML (8%). This gene encodes a MAX-interacting protein and is believed to act as a transcription factor that suppresses MYC binding to its target. By in silico analysis, we found that MGA is expressed in normal myeloid hematopoietic cells and AML, and the expression level is comparable with TET2 or DNMT3A. Further data mining of TCGA revealed a high frequency of inactivating mutations of the MGA gene in a variety of cancers such as various adenocarcinomas. To interrogate functionally its role in leukemogenesis, lentiviral constructs containing either shRNA or CRISPR-sgRNA targeted to different regions of the MGA gene were generated. MGA expressing AML cell line EOL-1 was silenced by shRNA or CRISPER system. Silencing was confirmed by western blot (shRNA) and Sanger Sequencing (sgRNA). An increase of methylcellulose colony number (~30%) was observed in MGA silenced cell lines. Control ...
BioGenex eFISH AML1/ETO dual color dual fusion probe comes in hybridization buffer contains green-labeled polynucleotides (Green: excitation at 503 nm and emission at 528 nm, similar to FITC, which target the AML1 gene in 21q22, and orange-labeled polynucleotides (Orange: excitation at 547 nm and emission at 572 nm, similar to rhodamine), which target the ETO gene in 8q22. ...
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The mechanism underlying the lineage decision made by CD4(+)CD8(+) double-positive (DP) thymocytes that give rise to two T lymphocyte subset with distinct functionalities, that is, helper and cytotoxic T cells, remains a major issue in immunology. The lineage decision process involves several phases and terminates when cells loose their developmental plasticity to become the alternate lineage. A detailed picture of the transcription factor network governing helper versus cytotoxic-lineage decision has recently emerged. Studies published only past year provided new insights into how the expression of ThPOK, a central transcription factor for helper T cell development, is regulated. It has now become evident that an antagonistic interplay between ThPOK and Runx transcription factor complexes plays an essential role in thwarting an alternate fate during the commitment process.
Scientists from the Cancer Science Institute of Singapore (CSI Singapore) at the National University of Singapore (NUS) have discovered that modifications to a protein called RUNX3 may promote cancer progression. The results of the study were published in the prestigious journal Proceedings of the National Academy of Sciences (PNAS) in June 2016. The research team, led by Professor Yoshiaki Ito, Senior Principal Investigator at CSI Singapore, found that a modification called phosphorylation made to RUNX3 promotes cancer progression by allowing cell division. Uncontrolled cell division in the body is a process by which tumours form and hence is a hallmark of cancer. RUNX3 is a tumour suppressor gene that prevents the formation of tumours by binding to DNA. The phosphorylation, or the addition of a phosphate group to a molecule, is carried out by an enzyme called Aurora Kinase, which has been observed to be present in unusually high levels in some cancers. Phosphorylation prevents the binding of ...
Study hypothesis: Treatment with dasatinib 100 mg QD is safe and efficacious when given to patients with Ph+ ALL in the post SCT setting.
The inherited platelet disorders are an uncommon cause of symptomatic bleeding. They may be difficult to diagnose (and are likely to be under-diagnosed) and pose problems in management. This review discusses the inherited platelet disorders summarising the current state of the art with respect to in …
RUNX3 CRISPR Knockout and Activation Products (h) are ready to use gene editing systems designed to knockout or upregulate gene expression of human RUNX3
If you have a platelet disorder, you may not be able to form clots as easily. That means you could be at risk for excessive bleeding.
The RUNX1/AML1 gene is the most frequent target for chromosomal translocation in leukemia. In addition, recent studies have demonstrated point mutations in the RUNX1 gene as another mode of genetic alteration in development of leukemia. Monoallelic germline mutations in RUNX1 result in familial plat …
RUNX1 antibody (runt-related transcription factor 1) for ICC/IF, WB. Anti-RUNX1 pAb (GTX129100) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
RUNX1 antibody (runt-related transcription factor 1) for WB. Anti-RUNX1 pAb (GTX11903) is tested in Human, Mouse samples. 100% Ab-Assurance.
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AML-2 (Acute myeloid leukemia 2 or Runx3) antibody against the runt transcription factor 3 for use in supershift (EMSA) and Western blotting.
PHF2라는 단백질이 뼈를 만드는 세포(조골세포)를 활성화시킨다는 사실을 처음으로 규명했다. 조골세포는 Runx2라는 단백질에 의해 분화가 조절된다. 반면, SUV39HI1라는 효소는 Runx2에 메틸기(CH3)를 붙임으로써 Runx2가 기능을 하지 못하게 하는 장식으로 분화를 방해한다. 성장이 끝난 성인들이 더 이상 키가 크지 않는 것도 SUV39HI1 효소 때문이다. 이에 착안해 Runx2에 붙어 있는 메틸기를 제거하는 방안을 연구한 결과, PHF2 단백질이 조골세포 분화를 유도함으로써, 소아의 뼈 발달 과정이나 골절 후 뼈가 새로 형성되는 과정에 작용한다는 것을 증명했다.. PHF2 단백질은 Runx2에 붙어 있는 메틸기를 제거했으며, 이후 본연의 기능을 회복한 Runx2는 조골세포의 분화를 촉진하여 다시 뼈를 만들기 시작했다. 실제 유전자 조작으로 PHF2 단백질이 과발현된 쥐를 만들어 ...
Current Research and Scholarly InterestsInherited mutations in the RUNX1 gene cause a platelet disorder and increased risk of blood cancers. However, it is still unclear what actually causes progression to cancer in these patients. Using genetic editing, I am investigating how RUNX1 mutations contribute to disease. ...
Accountants have been warned to expect an increase in AML compliance work in the wake of a new report criticising bodies for a widespread lack of ML
Answers to FAQs from patients, which will answer any questions that they have. Dr. Stavros Alevrogiannis - MD-Msc-PhD, Tel. Contact: 2107786868.
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針法是把毫針刺入患者身體某一穴位,運用捻轉與提插等針刺手法來治療疾病;灸法是把燃燒著的艾絨按一定穴位熏灼皮膚,利用熱的刺激來治療疾病。針灸由針和灸構成,是中醫學的重要組成部分之一,其內容包括針灸理論、腧穴、針灸技術以及相關器具。在形成、應用和發展的過程中,具有鮮明的中國民族文化與地域特徵,是基於中國民族文化和科學傳統產生的寶貴遺產。中醫針灸的作用涉及到中醫針灸調節內分泌、愛民中醫針灸減肥等具體領域。. 中醫拔罐療法又稱角法,拔罐通過物理的刺激和負壓人為造成毛細血管破裂淤血,調動人體修復功能,及壞死血細胞吸收功能,能促進血液循環,激發精氣,調理氣血,達到提高和調節人體免疫力的作用。. ...
中元堂保健推拿手法,對於常見的筋骨扭傷,治理舊患,腰膝蓋踝勞損,腰椎間盤痛等等各類困擾日常生活的痛症皆有良好效果。 不少人因為外傷,勞責飽受身體疼痛的困擾,影響情緒。這個情況可到跌打醫師尋求止痛舒緩的推拿治療,對於長期痛症和急性痛症,只要是良好的按摩方法及正骨手法即可以大大減輕刺痛程度,持續舒緩止痛,對骨痛及神經痛特別有效 ...
椎間盤突出,非椎間盤突出導致的腰背部,頸部疼痛,坐骨神經痛,尾椎小關節綜合症,椎管狹窄症,脊椎側彎,姿勢不良,背部手術失敗症,頭痛,頸痛,肩週炎,腰背痛,脊柱側彎症,坐骨神經痛,膝痛,足踝痛,腳痛,骨刺,手腳麻痺,運動創傷,職業性損傷. 觀塘 ...
跌打中醫整脊正骨復位針灸拔罐痛症治療中心 - 中元堂(旺角醫舘)整脊正骨復位痛症治療中心介紹 │中元堂,不少香港人深受身體痛症困擾,本中心是一間專業痛症治療中心,提供即時痛症舒緩,骨傷正骨復位服務,即是以前稱之為【跌打】,俗稱鐵打的手法推拿理療。 常見痛症例如突然跌傷,拉傷,常見的筋骨扭傷,骨傷,舊患,長期腰痛,腰膝蓋踝勞損,腰椎間盤痛等等的痛症,推拿手法可以針對酸痛紅腫的部份處理,即時消腫止痛,幫助恢復健康。長期因為姿勢不良,運動外傷,先天遺傳等等的骨骼移位,可能自己不先知,建議以正骨手法及早糾正,傷勢嚴重不能走動者可以預約【跌打夜診】服務。本中心服務如下:骨傷手法理療(俗稱跌打)正骨復位痛症治療拔罐針灸
手腳扭傷,撞瘀手腳當然去睇跌打,去理療中心敷藥。由跌打師傅斷症後,配合推拿手法進行跌打程序,才可以把握保健黃金的時間將痛楚減到最低。跌打幾耐先會好?治療得當,最快一帖跌打藥膏,24小時內便可以康復了。 但是,找趺打師傅太心急的話,容易忽略以下10件事,導致身體情況惡化!下次看跌打/鐵打前,記得停一停,諗一諗!....
點解唔去搵一個適合自己既中醫教養生方法,學調理? 現在女性久坐不動,而且生活習慣不佳,我們眼中的女性調理,便是為她們找出適合她們自身體質的藥方,幫助她們順應四季,是謂《調理》,也是《養生》。
편집국: 서울특별시 동대문구 경희대로 26 (회기동) 경희대학교 의과대학 미생물학교실 239호 / Tel. (02)961- ...
你知道為什麼中國人有睇【跌打】的習慣嗎?【跌打】,又俗稱鐵打,是中國傳統中醫的其中一種利用跌打藥,跌打藥粉,跌打敷藥,加上中醫推拿的治療方法。優點是以推拿按摩手法而促進康復,藥帖直接接觸外皮,所以見效快,大部份時候不須要食藥,而且不需要以手術界入便可康復。 ...
teleDesign 2012 Nikolai Holzach , Haimhauserstr.3 , 80802 München , Tel: 0049(0)89-348967 , Responsable for the content § 55 II RStV : Nikolai Holzach ...
Runt-related transcription factor 1 (RUNX1) is generally considered to function as a tumor suppressor in the development of leukemia, but a growing body of evidence suggests that it has pro-oncogenic properties in acute myeloid leukemia (AML). Here we have demonstrated that the antileukemic effect mediated by RUNX1 depletion is highly dependent on a functional p53-mediated cell death pathway. Increased expression of other RUNX family members, including RUNX2 and RUNX3, compensated for the antitumor effect elicited by RUNX1 silencing, and simultaneous attenuation of all RUNX family members as a cluster led to a much stronger antitumor effect relative to suppression of individual RUNX members. Switching off the RUNX cluster using alkylating agent-conjugated pyrrole-imidazole (PI) polyamides, which were designed to specifically bind to consensus RUNX-binding sequences, was highly effective against AML cells and against several poor-prognosis solid tumors in a xenograft mouse model of AML without ...
TY - JOUR. T1 - Loss of runt-related transcription factor 3 induces gemcitabine resistance in pancreatic cancer. AU - Horiguchi, Shigeru. AU - Shiraha, Hidenori. AU - Nagahara, Teruya. AU - Kataoka, Jyunnro. AU - Iwamuro, Masaya. AU - Matsubara, Minoru. AU - Nishina, Shinichi. AU - Kato, Hironari. AU - Takaki, Akinobu. AU - Nouso, Kazuhiro. AU - Tanaka, Takehiro. AU - Ichimura, Koichi. AU - Yagi, Takahito. AU - Yamamoto, Kazuhide. PY - 2013/8. Y1 - 2013/8. N2 - Background & Aim: Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene that is expressed in gastric and other cancers including pancreatic cancer. However, the precise function of RUNX3 in pancreatic cancer has not been fully elucidated. In this study, we aimed to determine the effect of decreased RUNX3 expression in pancreatic cancer. Methods: This study included 36 patients with primary pancreatic cancer, who had undergone pancreaticoduodenectomy. All patients were treated with 1000mg/m2 gemcitabine after the surgery. ...
Runt-related transcription factor 1 (RUNX1) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters and can accelerate apoptosis in various tumors. However, the regulatory mechanisms underlying RUNX1 expression in neuroblastoma (NB), a highly malignant tumor in childhood, remain largely unclear. In this study, we aimed to assess the role of RUNX1 in NB and to reveal the underlying mechanisms that may contribute to finding a potential therapeutics strategy against NB. Growth, invasion, metastasis and angiogenesis were assessed using Cell Counting Kit-8 (CCK-8) immunocytochemistry, and studies involving soft agar, cell invasion, tube formation and whole animals. The levels of expression were measured using real-time quantitative PCR for RNA, Western blot and immunostaining analyses for proteins. Luciferase reporter and chromatin immunoprecipitation assays indicated that RUNX1 directly binds within the BIRC5, CSF2RB and NFKBIA promoter regions to facilitate
Hematopoietic stem cells (HSCs) are self-renewing multipotent stem cells that generate mature blood lineages throughout life. They, together with hematopoietic progenitor cells (collectively known as HSPCs), emerge from hemogenic endothelium in the floor of the embryonic dorsal aorta by an endothelial-to-hematopoietic transition (EHT). Here we demonstrate that transforming growth factor β (TGFβ) is required for HSPC specification and that it regulates the expression of the Notch ligand Jagged1a in endothelial cells prior to EHT, in a striking parallel with the epithelial-to-mesenchymal transition (EMT). The requirement for TGFβ is two fold and sequential: autocrine via Tgfβ1a and Tgfβ1b produced in the endothelial cells themselves, followed by a paracrine input of Tgfβ3 from the notochord, suggesting that the former programs the hemogenic endothelium and the latter drives EHT. Our findings have important implications for the generation of HSPCs from pluripotent cells in vitro.
BACKGROUND: Runx transcription factors play critical roles in the developmental control of cell fate and contribute variously as oncoproteins and tumor suppressors to leukemia and other cancers. To discover fundamental Runx functions in the cell biology of animal development, we have employed morpholino antisense-mediated knockdown of the sea urchin Runx protein SpRunt-1. Previously we showed that embryos depleted of SpRunt-1 arrest development at early gastrula stage and underexpress the conventional protein kinase C SpPKC1. RESULTS: We report here that SpRunt-1 deficiency leads to ectopic cell proliferation and extensive apoptosis. Suppression of the apoptosis by pharmacological inhibition of caspase-3 prevents the ectopic proliferation and rescues gastrulation, indicating that many of the overt defects obtained by knockdown of SpRunt-1 are secondary to the apoptosis. Inhibition or knockdown of SpPKC1 also causes apoptosis, while cell survival is rescued in SpRunt-1 morphant embryos coinjected with
RUNX transcription factors belong to a highly conserved class of transcriptional regulators which play various roles in the development of the majority of metazoans. In this review we focus on the founding member of the family, RUNX1, and its role in the transcriptional control of blood cell development in mammals. We summarize data showing that RUNX1 functions both as activator and repressor within a chromatin environment, a feature that requires its interaction with multiple other transcription factors and co-factors. Furthermore, we outline how RUNX1 works together with other factors to reshape the epigenetic landscape and the three-dimensional structure of gene loci within the nucleus. Finally, we review how aberrant forms of RUNX1 deregulate blood cell development and cause hematopoietic malignancies. ...
The Runt related transcription factors (RUNX) are recognized as key players in suppressing or promoting tumor growth. RUNX3, a member of this family, is known as a tumor suppressor in many types of cancers, although such a paradigm was challenged by some researchers. The TGF-β pathway governs major upstream signals to activate RUNX3. RUNX3 protein consists of several regions and domains. The Runt domain is a conserved DNA binding domain and is considered as the main part of RUNX proteins since. Herein, we compared the effects of Runt domains and full-Runx3 in cell viability by designing two constructs of Runx3, including N-terminal region and Runt domain. We investigated the effect of full-Runx3, N-t, and RD on growth inhibition in AGS, MCF-7, A549, and HEK293 cell lines which are different in TGF-β sensitivity, in the absence and presence of TGF-β. The full length RUNX3 did not notably inhibit growth of these cell lines while, the N-t and RD truncates showed different trends in these cell lines.
TY - JOUR. T1 - Fusion AML1 transcript in a radiation-associated leukemia results in a truncated inhibitory AML1 protein. AU - Hromas, Robert. AU - Busse, Tracey. AU - Carroll, Audra. AU - Mack, David. AU - Shopnick, Rinah. AU - Zhang, Dong Er. AU - Nakshatri, Harikrishna. AU - Richkind, Kathleen. PY - 2001/4/1. Y1 - 2001/4/1. N2 - AML1 is a transcription factor that is essential for normal hematopoietic development. It is the most frequent target for translocations in acute leukemia. Recently, fluorescence in situ hybridization was used to identify a novel syndrome of radiation-associated secondary acute myelogenous leukemia that had AML1 translocations. Using polymerase chain reaction, the AML1 fusion transcript was isolated from the patient who had a t(19;21) radiation-associated leukemia. The AML1 gene is fused out of frame to chromosome 19 sequences, resulting in a truncated AML protein bearing the DNA binding domain but not the transcriptional activation domain. This fusion AML1 protein ...
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The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
Cancer researchers at the University of Cincinnati College of Medicine have found an obesity-associated proteins role in leukemia development and drug response which could lead to more effective therapies for the illness.
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This resource is intended for patients with acute myeloid leukemia (AML). You will find expert advice about AML to help you discuss key issues with your healthcare provider and make important decisions related to management and treatment. Easy-to-understand animations with audio narration, expert video explanations, illustrated slide shows, and educational downloads are available to you.
This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must be interpreted in the context of a patients clinical data using reasonable medical judgment. This website should not be used as a substitute for the advice of a licensed physician ...
The algorithm predicts the chance of the achievement of a complete remission and the risk for an early death for elderly patients (60 years and older) with newly diagnosed AML.
Protein CBFA2T3 (core-binding factor, runt domain, alpha subunit 2; translocated to, 3) is a protein that in humans is encoded ... "Entrez Gene: CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3". Hoogeveen AT, Rossetti S, ... Calabi F, Cilli V (Dec 1998). "CBFA2T1 (core-binding factor, runt domain, alpha subunit 2; translocated to, 3), a gene ... and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi ...
"Entrez Gene: CBFA2T2 core-binding factor, runt domain, alpha subunit 2; translocated to, 2". Rual JF, Venkatesan K, Hao T, ... The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several ... 18 (2): 846-58. doi:10.1128/MCB.18.2.846. PMC 108796. PMID 9447981. Fracchiolla NS, Colombo G, Finelli P, Maiolo AT, Neri A ( ... 241 (2): 287-95. doi:10.1016/S0378-1119(99)00481-3. PMID 10675041. Hoogeveen AT, Rossetti S, Stoyanova V, Schonkeren J, ...
... also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that in humans is encoded by the RUNX2 gene. RUNX2 ... The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants ... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for ... The phosphorylation state of Runx2 also mediates its DNA-binding activity. The Runx2 DNA-binding activity is correlated with ...
... or core-binding factor subunit alpha-2 (CBFA2) is a protein that in humans is encoded by the RUNX1 gene. RUNX1 is a ... a DNA binding CBFα chain (RUNX1 or RUNX2) and a non-DNA-binding subunit called core binding factor β (CBFβ); the binding ... Wang, S, Speck, NA (January 1992). "Purification of core-binding factor, a protein that binds the conserved core site in murine ... It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). ...
Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. It was previously believed that the RNA ... Sigma factors in E. coli:. *σ70(RpoD) - σA - the "housekeeping" sigma factor or also called as primary sigma factor, ... Different sigma factors are utilized under different environmental conditions. These specialized sigma factors bind the ...
... it allosterically enhances DNA binding by the alpha subunit as the complex binds to the core site of various enhancers and ... Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family ...
The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ... Transcription initiation factor TFIID subunit 11 also known as TAFII28, is a protein that in humans is encoded by the TAF11 ... The conserved region contains four alpha helices and three loops arranged as in histone H3. TAF11 has been shown to interact ... In molecular biology, TAFII28 refers to the TATA box binding protein associated factor. Together with the TATA-binding protein ...
... later showed that the sigma factor does not obligatorily leave the core. Instead, it changes its binding with the core during ... subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. It was previously believed that the RNA ... Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... Therefore, the sigma factor, cycles between a strongly bound state during initiation and a weakly bound state during elongation ...
This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that ... pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding ... TFIIA-alpha and beta-like factor is a protein that in humans is encoded by the GTF2A1L gene. The assembly and stability of the ... "Entrez Gene: ALF TFIIA-alpha/beta-like factor". Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap ...
Selective factor 1 is composed of the TATA-binding protein and three TAF (TATA box-binding protein-associated factor) subunits ... It contains two short alpha helices and a long central alpha helix. TAF1 (TAFII250) TAF2 (CIF150) TAF3 (TAFII140) TAF4 ( ... for example the downstream promoter element or gene-specific core promoter sequence Due to such interactions, they contribute ... The TBP-associated factors (TAF) are proteins that associate with the TATA-binding protein in transcription initiation. It is a ...
DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... "New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ...
These core binding factors, or nuclear factors (NF-Y), are composed of three subunits - NF-YA, NF-YB, and NF-YC. Whereas in ... The first domain (A1) contains 20 amino acids that forms an alpha helix that appears significant in its interactions with NF-YB ... It is essential to the transcription that these core binding factors (also referred to as nuclear factor Y or NF-Y) are able to ... the core binding factor (CBF)-DNA complex retains a high degree of conservation within the CCAAT binding motif, as well as the ...
DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-13. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... RNA polymerase holoenzymes containing other sigma factors recognize different core promoter sequences. <-- upstream downstream ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An inactive enhancer may be bound by an inactive transcription factor. Phosphorylation of the transcription factor may activate ...
One species that looked at is Drosophila, and in the subunits of the Drosophila transcription initiation factor has specific ... conserved motif found near the C-terminus in every core histone sequence in a histone octamer responsible for the binding of ... The histone fold averages about 70 amino acids and consists of three alpha helices connected by two short, unstructured loops. ... Also the histone fold was first found in TATA box-binding protein-associated factors, which is a main component in ...
This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 ... Chen Y, Le Cahérec F, Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... Knight BC, High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...
... subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, ... RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa, a beta (β) ... The core enzyme has five subunits (~400 kDa): β': The β' subunit is the largest subunit, and is encoded by the rpoC gene. The β ... The ω subunit facilitates assembly of RNAP and stabilizes assembled RNAP. In order to bind promoters, RNAP core associates with ...
The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ... "Structure-function analysis of the estrogen receptor alpha corepressor scaffold attachment factor-B1: identification of a ... This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and ... TATA-binding protein-associated factor 2N is a protein that in humans is encoded by the TAF15 gene. Initiation of transcription ...
Chen Y; Le Cahérec F; Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. GRCh38: ... Knight BC; High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ...
"BRCA1 augments transcription by the NF-kappaB transcription factor by binding to the Rel domain of the p65/RelA subunit". The ... Karetsou Z, Kretsovali A, Murphy C, Tsolas O, Papamarcaki T (April 2002). "Prothymosin alpha interacts with the CREB-binding ... hydrophobic amino acids in the interaction between the glucocorticoid receptor tau 1-core activation domain and target factors ... "Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) ...
In normoxia, HIF alpha subunits are marked for the ubiquitin-proteasome degradation pathway through hydroxylation of proline- ... "Structural basis for binding of hypoxia-inducible factor to the oxygen-sensing prolyl hydroxylases". Structure. 17 (7): 981-9. ... The catalytic domain consists of a double-stranded β-helix core that is stabilized by three α-helices packed along the major β- ... X-ray crystallography and NMR spectroscopy showed that both peptides bind to the same binding site on PHD2, in a cleft on the ...
"The alpha-like RNA polymerase II core subunit 3 (RPB3) is involved in tissue-specific transcription and muscle differentiation ... cooperation with promoter-bound activator domains and binding to TFIIB". J. Mol. Biol. 261 (5): 599-606. doi:10.1006/jmbi. ... "HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors". Genes Dev. 6 (4): 655-66. ... The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of ...
This contains a core of two compact domains with each having five alpha helices. The first five-helix bundle is a conserved ... Viral cyclin D binds human Cdk6 and inhibits Rb by phosphorylating it, resulting in free transcription factors which result in ... A simplification in yeast is that all cyclins bind to the same Cdc subunit, the Cdc28. Cyclins in yeast are controlled by ... A role for cAMP response element-binding protein and activating transcription factor-2 in pp60(v-src) signaling in breast ...
... a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 ... "The RTP site shared by the HIV-1 Tat protein and the 11S regulator subunit alpha is crucial for their effects on proteasome ... It also binds closely to the E3 ubiquitin ligase MDM2, which is a regulator of the degradation of p53 and retinoblastoma ... Accordingly, gene expression by degradation of transcription factors, such as p53, c-jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, ...
This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several ... The protein complex that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to ... "Induced alpha helix in the VP16 activation domain upon binding to a human TAF". Science. 277 (5330): 1310-3. doi:10.1126/ ... TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa, also known as TAF9, is a protein that in ...
The smaller subunit of this Damaged DNA Binding protein complex is known as DDB2 and is able to directly bind DNA lesions ... Recent reports show that IMiDs bind to CRL4CRBN and promote the degradation of IKZF1 and IKZF3 transcription factors, which are ... RBX1 is a core component of Cullin-RING ubiquitin ligase (CRL) complexes and functions to recruit E2 ubiquitin conjugating ... CUL4A protein is 759 amino acids long and forms an extended, rigid structure primarily consisting of alpha-helices. At the N- ...
"The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative ... which interacts with the seven-membered alpha ring of 20S core particle and eastablishs an asymmetric interface between the 19S ... It also have subunits that can bind with nucleotides (e.g., ATPs) in order to facilitate the association between 19S and 20S ... These subunits can be categorized into two classes based on the ATP dependence of subunits, ATP-dependent subunits and ATP- ...
"Multidomain organization of eukaryotic guanine nucleotide exchange translation initiation factor eIF-2B subunits revealed by ... The structure can be divided into a structural C-terminal core onto which the two N-terminal helices are attached. The core ... The W2 domain has a globular fold and is exclusively composed out of alpha-helices. ... the eIF-W2 domain functions as the binding site for Mnk eIF4E kinase, an enzyme that phosphorylates eukaryotic initiation ...
Törö I, Thore S, Mayer C, Basquin J, Séraphin B, Suck D (May 2001). "RNA binding in an Sm core domain: X-ray structure and ... RNA oligonucleotides generally bind inside the hole (lumen) of the LSm torus, one nucleotide per LSm subunit, but additional ... The short (two to four turns) N-terminal alpha helix occurs at one edge of the beta sandwich. This alpha helix and the beta ... the Sm-like protein HF-I encoded by the gene hfq was described in 1968 as an essential host factor for RNA bacteriophage Qβ ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
... general transcription factor 2H subunit 5) is also known as the TTD group A (TTDA) subunit (and as Tfb5). The TTDA subunit is ... and part of a six-subunit complex of Rad3, Tfb1, Tfb2, Tfb4, Tfb5, and Ssl1 (referred to as core) In humans, the function of ... TTDA is present both bound to TFIIH, and as a free fraction that shuffles between the cytoplasm and nucleus; induction of NER- ... 2012). "Subunit architecture of general transcription factor TFIIH". Proc Natl Acad Sci U S A. 109 (6): 1949-54. Bibcode: ...
... caused by the alpha decay of actinium-227.[35] Perey then attempted to determine the proportion of beta decay to alpha decay in ... Gottschlich, Michele M. (2001). The Science and Practice of Nutrition Support: A Case-based Core Curriculum. Kendall Hunt. p. ... The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... Sodium tetraphenylborate can also be classified as an organosodium compound since in the solid state sodium is bound to the ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
protein kinase binding. • core promoter binding. • RNA polymerase II transcription coactivator activity. • transcription factor ... ribosomal large subunit binding. • protein homodimerization activity. • ribosomal small subunit binding. • unfolded protein ... regulation of eIF2 alpha phosphorylation by dsRNA. • positive regulation of cell cycle G2/M phase transition. • negative ... activating transcription factor binding. • rRNA binding. • protein N-terminus binding. • chromatin binding. ...
BDNF is made in the endoplasmic reticulum and secreted from dense-core vesicles. It binds carboxypeptidase E (CPE), and the ... regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits ... receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ... BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor. ...
... a 9-subunit base that binds directly to the α ring of the 20S core particle, and a 10-subunit lid. Six of the nine base ... Degradation of Aux/IAA proteins derepresses transcription factors in the auxin-response factor (ARF) family and induces ARF- ... which serve as docking domains for the regulatory particles and the alpha subunits N-termini (Pfam PF10584) form a gate that ... The mechanisms by which it binds to the core particle through the C-terminal tails of its subunits and induces α-ring ...
The alpha helical anticodon binding domain of Arginyl, Glycyl and Cysteinyl-tRNA synthetases is known as the DALR domain after ... Alignment of the core domains of aminoacyl-tRNA synthetases class I and class II. Essential binding site residues (Backbone ... It aminoacylates at the 3'-OH of a terminal adenosine on tRNA, and is usually dimeric or tetrameric (two or four subunits, ... and an anticodon binding domain (which interacts mostly with the anticodon region of the tRNA and ensures binding of the ...
... s can bind to other proteins as well as to small-molecule substrates. When proteins bind specifically to other copies of ... Kauzmann W (May 1956). "Structural factors in protein denaturation". Journal of Cellular Physiology. 47 (Suppl 1): 113-31. doi: ... Quaternary structure: the structure formed by several protein molecules (polypeptide chains), usually called protein subunits ... The membrane alone has a hydrophobic core through which polar or charged molecules cannot diffuse. Membrane proteins contain ...
... the alpha helix). Linus Pauling was the first to identify[29] the 3.6 amino acids per helix turn ratio of the alpha helix. ... It, however, requires a five base binding between the mRNA and tRNA with a flip of the anti-codon creating a triplet coding, ... Such a code might be "degenerate", with 4×4×4=64 possible triplets of the four nucleotide subunits while there were only 20 ... In 1960, Crick accepted an honorary fellowship at Churchill College, Cambridge, one factor being that the new college did not ...
... the position where the substrate binds, may influence factors like binding affinity of ligands, stabilization of substrates ... The number of sub-units making up cellulosomes can also determine the rate of enzyme activity.[12] ... McCleary BV (November 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ... construction of the catalytic core, expression, and X-ray structure at 1.75 Å resolution". Biochemistry. 36 (51): 16032-9. doi: ...
Each tropomyosin molecule has a smaller calcium-binding protein called troponin bound to it. All thin filaments are attached to ... The filaments are organized into repeated subunits along the length of the myofibril. These subunits are called sarcomeres. ... They run through the core of each thick filament and anchor it to the Z-line, the end point of a sarcomere. Titin also ... Troponin and the associated tropomyosin undergo a conformational change after calcium binding and expose the myosin binding ...
Each AMPAR has four sites to which an agonist (such as glutamate) can bind, one for each subunit.[5] The binding site is ... Armstrong N, Sun Y, Chen GQ, Gouaux E (October 1998). "Structure of a glutamate-receptor ligand-binding core in complex with ... "Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin- ... "Postsynaptic factors in the expression of long-term potentiation (LTP): increased glutamate receptor binding following LTP ...
... sandwiched between the Walker A and B motifs of one subunit and the LSGGQ motif of the other subunit. The maltose binding ... heme-binding protein, and alkaline protease), heme, hydrolytic enzymes, S-layer proteins, competence factors, toxins, ... In addition, a gap in the protein is accessible directly from the hydrophobic core of the inner leaflet of the membrane bilayer ... The T domains are each built of typically 10 membrane spanning alpha helices, through which the transported substance can cross ...
This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding ... C1QBP has been shown to interact with Protein kinase D1,[10] BAT2,[11] PRKCD,[10] PKC alpha[10] and Protein kinase Mζ.[10] ... "Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation". J Clin ... transcription factor binding. • protein binding. • hyaluronic acid binding. • kininogen binding. • mitochondrial ribosome ...
Each subunit consists of three domains. At the carboxyl terminal of the peptide chain there's a short alpha helix domain that ... oxygen binding. • metal ion binding. • dopamine binding. • enzyme binding. • oxidoreductase activity. • iron ion binding. • ... response to growth factor. • response to ethanol. • cellular response to glucose stimulus. • phthalate metabolic process. • ... allows tetramerization.[15] The central ~300 amino acids make up a catalytic core, in which all the residues necessary for ...
... is the gene encoding the ε subunit of DNA polymerase III in Escherichia coli.[1] The ε subunit is one of three core ... of the gene product binds the θ subunit and carries out the exonuclease function and the C-terminus binds the α subunit ... "The proofreading exonuclease subunit epsilon of Escherichia coli DNA polymerase III is tethered to the polymerase subunit alpha ... The ε subunit is stabilized by the θ subunit within the complete polymerase complex.[7] ...
transcription factor activity, sequence-specific DNA binding. • transcription factor binding. • RNA polymerase II core promoter ... 2009). "The correlation of hepatocyte nuclear factor 4 alpha and 3 beta with hepatitis B virus replication in the liver of ... "Foxa2 and MafA regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression". J. Mol. ... transcription regulatory region DNA binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. ...
14 alpha particles) is easily produced from lighter nuclei in the alpha process in nuclear reactions in supernovae (see silicon ... The inner core of the Earth is generally presumed to consist of an iron-nickel alloy with ε (or β) structure.[10] ... Hemoglobin bound to carbon monoxide is known as carboxyhemoglobin. This effect also plays a minor role in the toxicity of ... The ensuing availability of inexpensive iron was one of the factors leading to the Industrial Revolution. Toward the end of the ...
InaD contains five binding domains called PDZ domain proteins, which specifically bind the C termini of target proteins. ... Drosophila differentially expresses a core group of 252 genes upon infection with most bacteria. This core group of genes is ... Bakker K (1961). "An analysis of factors which determine success in competition for food among larvae of Drosophila ... A 2016 study concluded that food supplementation with 10-mM alpha-ketoglutarate decreased Drosophila alcohol sensitivity over ...
The activated Gs alpha subunit binds to and activates an enzyme called adenylyl cyclase, which, in turn, catalyzes the ... crystallised the PKA Cα subunit, which revealed the bi-lobe structure of the protein kinase core for the very first time, ... Long term memory is dependent on the CREB transcription factor, regulated by PKA. A study done on drosophila reported that an ... Two cAMP molecules bind to each PKA regulatory subunit. *The regulatory subunits move out of the active sites of the catalytic ...
In the presence of the R subunit, the complex can also act as an endonuclease, binding to the same target sequence but cutting ... A conserved enzyme core with variable target-recognizing domains". J. Mol. Biol. 206 (2): 305-12. doi:10.1016/0022-2836(89) ... hsdM contains an alpha-helical domain at the N-terminus, the HsdM N-terminal domain.[7] ... The type I restriction and modification system is composed of three polypeptides R, M and S. The M (hsdM) and S subunits ...
These subunits surround the mRNA strand. The larger subunit contains three binding sites: A (aminoacyl), P (peptidyl), and E ( ... Transcription is regulated in the cell via transcription factors. Transcription factors are proteins that bind to regulatory ... The two reacting molecules are the alpha amino group of one amino acid and the alpha carboxyl group of the other amino acids. A ... In a hydrophilic environment such as cytosol, the hydrophobic amino acids will concentrate at the core of the protein, while ...
ubiquitin protein ligase binding. • importin-alpha family protein binding. • disordered domain specific binding. • core ... DNA binding. • core promoter binding. • transcription factor activity, sequence-specific DNA binding. • transcription ... Within each subunit, there are a variety of protein binding sites, as well as a total of 15 possible phosphorylation sites. ... transcription factor binding. • RNA polymerase II activating transcription factor binding. • phosphoprotein binding. • kinase ...
An important factor in the binding of the first generation NNRTIs, such as nevirapine, is the butterfly-like shape. Despite ... The cloroindole part interacted with the hydrophobic core of the pocket and influenced the binding mode of the R120393 so it ... Part 1: From alpha-anilinophenylacetamide (alpha-APA) to imidoyl thiourea (ITU)". Bioorganic & Medicinal Chemistry Letters. 11 ... Å from the catalytic site in the palm domain of the p66 subunit site of the enzyme. The NNRTI binding pocket (NNIBP) contains ...
Most vegetable oils are rich in linoleic acid (safflower, sunflower, and corn oils). Alpha-linolenic acid is found in the green ... Malinauskas T (2008). "Docking of fatty acids into the WIF domain of the human Wnt inhibitory factor-1". Lipids. 43 (3): 227-30 ... The steroids, all derived from the same fused four-ring core structure, have different biological roles as hormones and ... Polyketides are synthesized by polymerization of acetyl and propionyl subunits by classic enzymes as well as iterative and ...
The same protein may act as the subunit of both the pentamers and hexamers or they may be composed of different proteins.[81]. ... When the adaptive immune system of a vertebrate encounters a virus, it produces specific antibodies that bind to the virus and ... Counting these areas and multiplying by the dilution factor allowed him to calculate the number of viruses in the original ... Safety, tolerability and efficacy of peginterferon alpha-2a and ribavirin in chronic hepatitis C in clinical practice: The ...
0001204 DNA-binding transcription factor activity. • 5'-deoxyribose-5-phosphate lyase activity. • transcription factor binding ... "Functional interaction between the DNA binding subunit trimerization domain of NF-Y and the high mobility group protein HMG-I(Y ... core motif, a novel cross-linking domain located between the second and third AT-hook, and a C-terminal acidic tail ... retinoic acid receptor binding. • enzyme binding. • peroxisome proliferator activated receptor binding. • DNA binding. • GO: ...
Assembly factor proteins[31] 47. NDUFAF1c. CIA30_HUMAN. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex, assembly factor 1. ... Core Subunitsa 1. NDUFS7 / PSST / NUKM. NDUS7_HUMAN. NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial EC ... Complex I contains a ubiquinone binding pocket at the interface of the 49-kDa and PSST subunits. Close to iron-sulfur cluster ... 1 alpha subcomplex subunit 3. Pfam PF14987 39. NDUFA4 / MLRQc. NDUA4_HUMAN. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex ...
Zipper Coiled-Coil Domains of the cGMP-Dependent Protein Kinase I alpha and its Interaction with the Myosin Binding Subunit of ... Leucine zipper coiled-coil helices & DNA-binding helices: transcription factor Max (PDB file 1HLO) ... in the hydrophobic core, and one containing predominantly polar amino acids oriented toward the solvent-exposed surface of the ... DNA bindingEdit. α-Helices have particular significance in DNA binding motifs, including helix-turn-helix motifs, leucine ...
X-ray crystallographic structure of the 30S ribosomal subunit with bound drug (purple, space-filling model, at center) protein ... Volume 1. Core Clinical Skills. Oxford University Press. 2010. *^ referenced in Treatment of Mitochodrial Disease: Bindu LH, ... PubMed:19031229] See Also Fischel-Ghodsian N. Genetic factors in aminoglycoside toxicity. Ann NY Acad Sci. 1999; 884:99-109. [ ... secondary structure elements such as alpha-helices in bright green, and the RNA phosphodiester backbone shown in orange (and ...
... alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2, (10ug), 10 µg. ... Home » cDNA » Mouse cDNA » Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 ( ... MC219266 Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), ... Properties for Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) ( ...
Subunit. /*genetics Core. Binding. Factor. Alpha. 3 Subunit. /*genetics Precursor Cells, T-Lymphoid/*immunology T-Lymphocytes/* ... Subunit. /immunology ; Core. Binding. Factor. Alpha. 3 Subunit. /immunology ; Female ; Gene Expression Profiling ; Gene ... Factor. -. alpha. /*metabolism Wet Macular Degeneration/*metabolism. Animals ; Cells, Cultured ; Core. Binding. Factor. Alpha. ... Subunit. /*genetics Non-alcoholic Fatty Liver Disease/*genetics Obesity, Morbid/*genetics. Adult ; Core. Binding. Factor. Alpha ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 1; cyclin D-related ... The protein produced from the normal RUNX1 gene is part of a protein complex known as core binding factor (CBF). As part of CBF ... Core binding factor acute myeloid leukemia. A rearrangement (translocation) of genetic material involving the RUNX1T1 gene is ... Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. Int J Hematol. 2011 Aug;94(2): ...
... signalling molecules and transcription factors. Definitive HSCs derived during embryogenesis in the aorta-gonad-mesonephros ... Core Binding Factor Alpha 2 Subunit * Proto-Oncogene Proteins c-myb * Zebrafish Proteins ... Haematopoietic stem cell (HSC) homeostasis is tightly controlled by growth factors, signalling molecules and transcription ... factors. Definitive HSCs derived during embryogenesis in the aorta-gonad-mesonephros region subsequently colonize fetal and ...
Core Binding Factor Alpha 2 Subunit * DNA-Binding Proteins / genetics* * DNA-Binding Proteins / metabolism ... Most of the RUNX1 mutations are clustered in the Runt domain and result in defective DNA binding but active beta-subunit ... binding, which is consistent with three-dimensional structural findings and may explain the dominant inhibitory effects. Unlike ...
... while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of ... The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus ... CBF complexes repress ZBTB7B transcription factor during cytotoxic (CD8+) T cell development. They bind to RUNX-binding ... RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5-TGTGGT ...
SL3-3 enhancer factor 1 alpha A subunit. *SL3/AKV core-binding factor alpha A subunit ... The RUNX2 protein is a transcription factor, which means it attaches (binds) to specific regions of DNA and helps control the ... 2013 Feb 7;92(2):252-8. doi: 10.1016/j.ajhg.2012.12.001. Epub 2013 Jan 3. Citation on PubMed or Free article on PubMed Central ... 2012 Mar;18(2):184-90. doi: 10.1111/j.1601-0825.2011.01862.x. Epub 2011 Oct 24. Citation on PubMed ...
Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (Predicted). ... PREDICTED: transcription initiation factor TFIID subunit 4-like[Cricetulus griseus].. 11. Mus musculus. 12396. Cbfa2t2; ... PREDICTED: transcription initiation factor TFIID subunit 4B-like[Cricetulus griseus].. 10. Cricetulus griseus. 100769929. ... PREDICTED: transcription initiation factor TFIID subunit 4-likeisoform 1 [Bombus impatiens].. 31. Pteropus vampyrus. ...
SL3/AKV core binding factor alpha B subunit. *SL3/AKV core-binding factor alpha B subunit ... The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes ... CBF binds to the core site, 5-PYGPYGGT-3, of a number of enhancers and promoters, including murine leukemia virus, ... SL3 3 enhancer factor 1 alpha B subunit. *SL3-3 enhancer factor 1 alpha B subunit ...
Core Binding Factor Alpha 1 Subunit); 0 (Drugs, Chinese Herbal); 0 (RUNX2 protein, human). ... Subunidade alfa 1 de Fator de Liga o ao Core/gen tica. Seres Humanos. Sistema de Sinaliza o das MAP Quinases/efeitos dos f ... 0 (Enzyme Inhibitors); EC 3.2.1.1 (alpha-Amylases); EC 3.4.21.62 (Subtilisin). ... Prote na Morfogen tica ssea 2/gen tica. Diferencia o Celular/efeitos dos f rmacos. C lulas Cultivadas. ...
0 (Core Binding Factor Alpha 1 Subunit); 0 (Glycation End Products, Advanced); 0 (Reactive Oxygen Species); 0 (Thiophenes); ... 0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Tumor Necrosis Factor-alpha); ... Subunidade alfa 1 de Fator de Liga o ao Core/metabolismo. Masculino. Modelos Biol gicos. Mi citos de M sculo Liso/efeitos dos f ... Factors affecting recurrence were also examined. RESULTS: A total of 5695 UC patients were registered. Among the 4677 patients ...
Compare CBFA2/RUNX1 translocation partner 2 ELISA Kits from leading suppliers on Biocompare. View specifications, prices, ... Core Binding Factor Alpha Subunit 2 Translocated To 2 (. *. Detection Range: 0.313 ng/ml - 20 ng/ml ... Background: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a rational target... read more ... CBFA2/RUNX1 translocation partner 2 ELISA Kits. The ELISA (enzyme-linked immunosorbent assay) is a well-established antibody- ...
CBFA2/RUNX1 Translocation Partner 2, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards ... core binding factor Runt domain,alpha subunit 2,(RUNX1) translocated to 2,mainly expressed in brain,with several isoforms due ... Core-binding factor, runt domain, alpha subunit 2; translocated to, 2:. *Hs.153934 ... Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 2 2 3 ...
CBFA2/RUNX1 Partner Transcriptional Co-Repressor 2, including: function, proteins, disorders, pathways, orthologs, and ... core binding factor Runt domain,alpha subunit 2,(RUNX1) translocated to 2,mainly expressed in brain,with several isoforms due ... Core-Binding Factor, Runt Domain, Alpha Subunit 2; Translocated To, 2 2 3 ... Transcription Factor. Binding Sites. Gene Targets. GH20J033400. Promoter/Enhancer. 2.1. EPDnew Ensembl ENCODE CraniofacialAtlas ...
AML2SL3/AKV core-binding factor alpha C subunit. *CBFA3. *CBFA3MGC16070. *CBF-alpha-3 ...
AML2SL3/AKV core-binding factor alpha C subunit. *CBFA3. *CBFA3MGC16070. *CBF-alpha-3 ...
Core binding factor runt domain alpha subunit 2 translocated to 1 cyclin D related antibody ... Core binding factor runt domain alpha subunit 2 translocated to 1 antibody ... Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. Can repress ... Runt related transcription factor 1 translocated to 1 antibody. *Runt related transcription factor 1 translocated to 1 cyclin D ...
Protein CBFA2T3 (core-binding factor, runt domain, alpha subunit 2; translocated to, 3) is a protein that in humans is encoded ... "Entrez Gene: CBFA2T3 core-binding factor, runt domain, alpha subunit 2; translocated to, 3". Hoogeveen AT, Rossetti S, ... Calabi F, Cilli V (Dec 1998). "CBFA2T1 (core-binding factor, runt domain, alpha subunit 2; translocated to, 3), a gene ... and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi ...
Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Assay Type: Probe Assay Design: Exonic Application: Gene ... Core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Assay Type: SYBR® Green Assay Design: Exonic Application ... Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting ... R2. ,0.99. These DNA primer pairs were designed by prioritizing the gene regions most commonly found in transcript variants. ...
"Entrez Gene: CBFA2T2 core-binding factor, runt domain, alpha subunit 2; translocated to, 2". Rual JF, Venkatesan K, Hao T, ... The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several ... 18 (2): 846-58. doi:10.1128/MCB.18.2.846. PMC 108796. PMID 9447981. Fracchiolla NS, Colombo G, Finelli P, Maiolo AT, Neri A ( ... 241 (2): 287-95. doi:10.1016/S0378-1119(99)00481-3. PMID 10675041. Hoogeveen AT, Rossetti S, Stoyanova V, Schonkeren J, ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 3. *Omim ID: ... and a brefeldin A-sensitive association of RII-alpha protein with one of the isoforms has been demonstrated in the Golgi ... MTG8-related gene 2,myeloid translocation gene on chromosome 16,zinc finger MYND domain-containing protein 4 ...
... alpha subunit 2; translocated to, 2) for WB. Anti-MTGR1 pAb (GTX16137) is tested in Human samples. 100% Ab-Assurance. ... core-binding factor, runt domain, alpha subunit 2; translocated to, 2. Background. Myeloid tumor related gene (MTGR1) is found ... For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple ...
SL3-3 enhancer factor 1 alpha B subunit; SL3/AKV core-binding factor alpha B subunit; transcription factor ... DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding transcription factor binding ... Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit 2; Oncogene AML-1; OTTHUMP00000108697; ... regulatory region DNA binding RNA polymerase II regulatory region sequence-specific DNA binding core promoter binding ...
Core Binding Factor Alpha 2 Subunit. *MicroRNAs. *DNA-Binding Proteins. *FISH. *TCF3 ... bHLH transcription factor binding - cell development - chromatin binding - cytoplasm - DNA binding - E-box binding - G1 phase ... transcription factor binding - transcription factor complex - transcription regulatory region DNA binding - transcription, DNA- ... sequence-specific DNA binding - sequence-specific DNA binding transcription factor activity - T cell differentiation in thymus ...
Core-binding factor subunit alpha-1; Short=CBF-alpha-1;AltName: Full=Oncogene AML-3;AltName: Full=Osteoblast-specific ... the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. Virology. 1993 May;194(1):314-31 ... J:19082 Ogawa E, et al., PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila ... J:49076 Xiao ZS, et al., Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor. ...
Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: SYBR® Green Assay Design: exonic ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Assay Design: exonic ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Application: Gene Expression ... Core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) Assay Type: Probe Application: Gene Expression ...
core-binding factor, runt domain, alpha subunit 2, translocated to, 2 (human) ... CBFA2T2 identified gene homolog,OTTMUSP00000016821,core-binding factor runt domain alpha subunit 2 translocated to 2 homolog, ... core-binding factor, runt domain, alpha subunit 2, translocated to, 2 homolog ... Novel binding partners of Ldb1 are required for haematopoietic development.. Meier N, Krpic S, Rodriguez P, Strouboulis J, ...
core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human). ... ccr4-not transcription complex, subunit 3. Cops2 12848. cop9 (constitutive photomorphogenic) homolog, subunit 2 (arabidopsis ... cbp/p300-interacting transactivator, with glu/asp-rich carboxy-terminal domain, 2. ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Calculated molecular weight: ... CBFA2T3, ETO2, hMTG16, MTG16, MTG8 related protein 2, MTGR2, Protein CBFA2T3, ZMYND4 ...
2. Fast two directional hand movements 3. Recommended for drawing curved hair stro ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 3 (CBFA2T3), transcript variant 2 ... Adenovirus with ORF of core-binding factor, runt domain, alpha subunit 2; translocated to, 2 (CBFA2T2), transcript variant 4 ... Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. ...
  • The protein produced from the normal RUNX1 gene is part of a protein complex known as core binding factor (CBF). (medlineplus.gov)
  • Most of the RUNX1 mutations are clustered in the Runt domain and result in defective DNA binding but active beta-subunit binding, which is consistent with three-dimensional structural findings and may explain the dominant inhibitory effects. (nih.gov)
  • Your search returned 5 CBFA2/RUNX1 translocation partner 2 ELISA ELISA Kit across 2 suppliers. (biocompare.com)
  • CBFA2T2 (CBFA2/RUNX1 Translocation Partner 2) is a Protein Coding gene. (genecards.org)
  • RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. (thermofisher.com)
  • runx1 expression in the lateral plate mesoderm co-localizes with the hematopoietic transcription factor scl, and expression of runx1 is markedly reduced in the zebrafish mutants spadetail and cloche. (zfin.org)
  • A common chromosomal translocation in acute myeloid leukemia (AML) involves the AML1 (acute myeloid leukemia 1, also called RUNX1, core binding factor protein (CBF alpha), and PEBP2 alpha B) gene on chromosome 21 and the ETO (eight-twenty one, also called MTG8) gene on chromosome 8. (curehunter.com)
  • the AML1 (show RUNX1 Antibodies )- ETO fusion protein increases the expression of SIRT1 (show SIRT1 Antibodies ), possibly by binding to the promoter region of SIRT1 (show SIRT1 Antibodies ) to activate its transcription in t(8;21) AML (show RUNX1 Antibodies ). (antibodies-online.com)
  • Transcription factors RUNX1 and RUNX3 in the induction and suppressive function of Foxp3+ inducible regulatory T cells. (nih.gov)
  • In this study, we show that the transforming growth factor-beta (TGF-beta) induces the expression of the Runt-related transcription factors RUNX1 and RUNX3 in CD4(+) T cells. (nih.gov)
  • This induction seems to be a prerequisite for the binding of RUNX1 and RUNX3 to three putative RUNX binding sites in the FOXP3 promoter. (nih.gov)
  • Inactivation of the gene encoding RUNX cofactor core-binding factor-beta (CBFbeta) in mice and small interfering RNA (siRNA)-mediated suppression of RUNX1 and RUNX3 in human T cells resulted in reduced expression of Foxp3. (nih.gov)
  • Web-based predictions of transcription factor binding revealed a binding site for runt-related transcription factor 1 (RUNX1), when the allele at the center of the palindrome (TGGGG(A/G)CCCCA) was A but not when it was G. Furthermore, electromobility shift assay (EMSA) presented evidence in support of allele-specific binding of RUNX1 to 5'HS4. (cdc.gov)
  • RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. (acris-antibodies.com)
  • Transcription factor Runx1 inhibits proliferation and promotes developmental maturation in a selected population of inner olfactory nerve layer olfactory ensheathing cells. (genscript.com)
  • Runx1 is a pivotal transcription factor required for HSC generation in the vascular regions of the mouse conceptus-the aorta, vitelline and umbilical arteries, yolk sac and placenta. (nih.gov)
  • The Runx1 transcription factor inhibits the differentiation of naive CD4+ T cells into the Th2 lineage by repressing GATA3 expression. (nih.gov)
  • In this study, we examined the functional role of the Runx1 transcription factor in Th cell differentiation. (nih.gov)
  • We previously established mouse lines that express a DNA binding domain of Runx1 from a transgene in a T-lineage-specific way (24). (nih.gov)
  • In the present study, we investigated the role of the Runx1 transcription factor in Th cell differentiation using these Runt-transgenic mice. (nih.gov)
  • [email protected]#To analyze the related factors affecting the long-term prognosis of acute myeloid leukemia (AML) children with positive RUNX1-RUNX1T1. (bvsalud.org)
  • Runx1/AML1 is a transcription factor implicated in tissue stem cell regulation and belongs to the small Runx family of cancer genes. (elsevier.com)
  • Here we show that the mouse transcription factor Runx1, a key regulator of myeloid cell proliferation and differentiation, is expressed in forebrain amoeboid microglia during the first two postnatal weeks. (unboundmedicine.com)
  • The transcription factor Runx1 plays a pivotal role in hematopoietic stem cell (HSC) emergence, and studies into its transcriptional regulation should give insight into the critical steps of HSC specification. (ox.ac.uk)
  • Endogenous Runx1 is transcribed from 2 alternative promoters, P1 and P2. (ox.ac.uk)
  • Runx1 is an important haematopoietic transcription factor as stressed by its involvement in a number of haematological malignancies. (cam.ac.uk)
  • Using an AGM-derived cell line as a model for the stromal microenvironment in the AGM and performing ChIP-Seq and ChIP-on-chip experiments, we demonstrate that Runx1, together with other key niche factors, is a direct target gene of Gata3. (cam.ac.uk)
  • In addition, we can pinpoint Gata3 binding to the Runx1 locus at specific enhancer elements which are active in the microenvironment. (cam.ac.uk)
  • It performs this function by attaching (binding) to proteins that normally turn genes on and blocking their activity. (medlineplus.gov)
  • Guo C, Hu Q, Yan C, Zhang J. Multivalent binding of the ETO corepressor to E proteins facilitates dual repression controls targeting chromatin and the basal transcription machinery. (medlineplus.gov)
  • E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. (cancerindex.org)
  • The specificity of tissue-restricted gene therapy can be refined by utilization of a 31-amino-acid segment of the hematopoietic and osteogenic AML/CBF transcription factors that direct the regulatory proteins to subnuclear sites that support gene expression. (umassmed.edu)
  • Sequences that support the intranuclear trafficking of AML/CBF transcription factors to subnuclear sites that support transcription were determined by the expression and visualization of mutated and epitope tagged AML/CBF proteins. (umassmed.edu)
  • Nuclear proteins that are localized in discrete domains within the nucleus include the leukemia-associated acute myelogenous leukemia (AML) and promyelocytic leukemia (PML) factors, the SC-35 RNA-processing factors, nucleolar proteins and components of both transcriptional and DNA replication complexes. (umassmed.edu)
  • Additionally we are shipping RUNX1T1 Proteins (6) and RUNX1T1 Kits (2) and many more products for this protein. (antibodies-online.com)
  • RUNX proteins are evolutionarily conserved transcription factors known to be involved in various developmental processes. (elsevier.com)
  • In studies on murine polyoma and leukemia viruses, mammalian core-binding factors (CBFs) were identified as proteins regulating viral replication (Boral et al. (springer.com)
  • It associates with DNA-binding transcription factors, other repressor proteins, and HISTONE ACETYLTRANSFERASES to repress expression of genes involved in cell growth and differentiation such as MATRIX METALLOPROTEINASE 7 and TCF12. (bvsalud.org)
  • The extracellular signal-related kinases 1 and 2 (ERK1/2) are key proteins mediating mitogen-activated protein kinase signaling downstream of RAS: phosphorylation of ERK1/2 leads to nuclear uptake and modulation of multiple targets. (ox.ac.uk)
  • Proteins belonging to the MADS family function as dimers, the primary DNA-binding element of which is an anti-parallel coiled coil of two amphipathic alpha-helices, one from each subunit. (ebi.ac.uk)
  • Different roles of E proteins in t(8;21) leukemia: E2-2 compromises the function of AETFC and negatively regulates leukemogenesis. (cancer-genetics.org)
  • Among these AETFC components, HEB and E2A, two members of the ubiquitously expressed E proteins, directly interact with AML1-ETO, confer new DNA-binding capacity to AETFC, and are essential for leukemogenesis. (cancer-genetics.org)
  • The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. (genecards.org)
  • PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene. (jax.org)
  • One of the genes that encodes a CBF alpha subunit is AML1, also called Cbf alpha 2. (curehunter.com)
  • In leukemic cells, AML1-ETO resides in and functions through a stable protein complex, AML1-ETO-containing transcription factor complex (AETFC), that contains multiple transcription (co)factors. (cancer-genetics.org)
  • However, the third E protein, E2-2, is specifically silenced in AML1-ETO-expressing leukemic cells, suggesting E2-2 as a negative factor of leukemogenesis. (cancer-genetics.org)
  • Indeed, ectopic expression of E2-2 selectively inhibits the growth of AML1-ETO-expressing leukemic cells, and this inhibition requires the bHLH DNA-binding domain. (cancer-genetics.org)
  • RUNX members modulate the transcription of their target genes through recognizing the core consensus binding sequence 5'-TGTGGT-3', or very rarely, 5'-TGCGGT-3', within their regulatory regions via their runt domain, while CBFB is a non-DNA-binding regulatory subunit that allosterically enhances the sequence-specific DNA-binding capacity of RUNX. (uniprot.org)
  • The RUNX2 protein is a transcription factor, which means it attaches (binds) to specific regions of DNA and helps control the activity of particular genes. (medlineplus.gov)
  • To identify genes whose expressions in primary human trabecular meshwork (TM) cell cultures are affected by the transcription factor pituitary homeobox 2 (PITX2) and to identify genes that may have roles in glaucoma. (molvis.org)
  • The five genes were DIRAS3 (DIRAS family, GTP-binding RAS-like 3), CXCL6 (chemokine (C-X-C motif) ligand 6), SAMD5 (sterile alpha motif domain containing 5), CBFB (core-binding factor, beta subunit), and MEIS2 (meis homeobox 2). (molvis.org)
  • Testing for multiple genes in a panel using NGS platforms is more practical and economical (2,3). (thefreelibrary.com)
  • These specialized sigma factors bind the promoters of genes appropriate to the environmental conditions, increasing the transcription of those genes. (wikipedia.org)
  • σ 70 (RpoD) - σ A - the "housekeeping" sigma factor or also called as primary sigma factor , transcribes most genes in growing cells. (wikipedia.org)
  • Every cell has a "housekeeping" sigma factor that keeps essential genes and pathways operating. (wikipedia.org)
  • These chromosomal abnormalities tend to disrupt genes that encode for transcription factors needed for myeloid stem cells to differentiate into specific blood components. (the-medical-dictionary.com)
  • SRF function is essential for transcriptional regulation of numerous growth-factor-inducible genes, such as c-fos oncogene and muscle-specific actin genes. (ebi.ac.uk)
  • In particular, studies show that E2-2 both redistributes AETFC to, and activates, some genes associated with dendritic cell differentiation and represses MYC target genes. (cancer-genetics.org)
  • Forms the heterodimeric complex core-binding factor (CBF) with CBFB. (uniprot.org)
  • Adenovirus with ORF of core-binding factor, beta subunit (CBFB), transcript variant 2 with C terminal Flag and His tag. (trademetro.net)
  • 1982 ). Further study revealed that PEBP2/CBFB was composed of dimers of a DNA-binding subunit (CBF-α) and a non-DNA-binding subunit (CBF-β). (springer.com)
  • Circulating human CD4(+) CD25(high) CD127(-) T reg cells significantly expressed higher levels of RUNX3, FOXP3, and TGF-beta mRNA compared with CD4(+)CD25(-) cells.Furthermore, FOXP3 and RUNX3 were colocalized in human tonsil T reg cells.These data demonstrate Runx transcription factors as a molecular link in TGF-beta-induced Foxp3 expression in iT reg cell differentiation and function. (nih.gov)
  • These data demonstrate Runx transcription factors as a molecular link in TGF-beta-induced Foxp3 expression in iT reg cell differentiation and function. (nih.gov)
  • They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. (dartmouth.edu)
  • A gene on chromosome 6p21 that encodes a nuclear protein member of the RUNX family of transcription factors with a Runt DNA-binding domain. (thefreedictionary.com)
  • Using chromatin immunoprecipitation in mouse embryonic fibroblasts and high-throughput sequencing, we find that ERF binds preferentially to elements away from promoters that contain RUNX or AP-1 motifs. (ox.ac.uk)
  • Parathyroid Hormone-responsive Smad3-related Factor, Tmem119, Promotes Osteoblast Differentiation and Interacts with the Bone Morphogenetic Protein-Runx2 Pathway. (jax.org)
  • This transcription factor is responsible for the differentiation of precursor cells into osteoblasts and regulates the differentiation of chondrocytes in the growth plate [ 4 ]. (springer.com)
  • Differentiation of naive CD4+ T cells into helper T (Th) cells is controlled by a combination of several transcriptional factors. (nih.gov)
  • RUNX2 is essential for osteoblastic differentiation and skeletal morphogenesis, and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. (thefreedictionary.com)
  • Human serum response factor (SRF) is a ubiquitous nuclear protein important for cell proliferation and differentiation. (ebi.ac.uk)
  • Foxo1 mediates insulin-like growth factor 1 (IGF1)/insulin regulation of osteocalcin expression by antagonizing Runx2 in osteoblasts. (jax.org)
  • MBS934869 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. (mybiosource.com)
  • Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. (mybiosource.com)
  • PREDICTED: transcription initiation factor TFIID subunit 4B-like[Cricetulus griseus]. (bioinformatics.org)
  • PREDICTED: transcription initiation factor TFIID subunit 4-likeisoform 1 [Bombus impatiens]. (bioinformatics.org)
  • Transcription factor TFIIE beta subunit, DNA-binding domain / TFA2 Winged helix domain 2 / TFA2, Winged helix domain 2 / TFIIE beta subunit core domain / Transcription initiation factor TFIIE, beta subunit / TFIIEalpha/SarR/Rpc3 HTH domain / TFE/IIEalpha-type HTH domain profile. (pdbj.org)
  • Domain structure of a human general transcription initiation factor, TFIIF. (labome.org)
  • [1] It is a bacterial transcription initiation factor that enables specific binding of RNA polymerase to gene promoters . (wikipedia.org)
  • The heterodimers bind to the core site of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL3 and GM-CSF promoters (Probable). (uniprot.org)
  • Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. (cancer-genetics.org)
  • Selection of promoters by RNA polymerase is dependent on the sigma factor that associates with it. (wikipedia.org)
  • The subnuclear organization of nucleic acids and cognate regulatory factors suggests that there are functional interrelationships between nuclear structure and gene expression. (umassmed.edu)
  • Mechanisms that control the spatial distribution of transcription factors within the three-dimensional context of the nucleus may involve the sorting of regulatory information, as well as contribute to the assembly and activity of sites that support gene expression. (umassmed.edu)
  • Molecular, cellular, genetic and biochemical approaches have identified distinct protein segments, termed intranuclear-targeting signals, that are responsible for directing regulatory factors to specific subnuclear sites. (umassmed.edu)
  • Gene rearrangements that remove or alter intranuclear-targeting signals are prevalent in leukemias and have been linked to altered localization of regulatory factors within the nucleus. (umassmed.edu)
  • Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2). (labome.org)
  • Identification of IFN regulatory factor-1 binding site in IL-12 p40 gene promoter. (labome.org)
  • Investigating the regulatory mechanism of viral replication led to the identification of the polyomavirus enhancer-binding protein 2 (PEBP2), also known as core-binding factor-β (CBF-β) (Katinka et al. (springer.com)
  • Molecular cloning and characterization of PEBP2 beta, the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. (jax.org)
  • Title: Runt-related transcription factor 1 regulates luteinized hormone-induced prostaglandin-endoperoxide synthase 2 expression in rat periovulatory granulosa cells. (genscript.com)
  • A redox factor protein, ref1, is involved in negative gene regulation by extracellular calcium. (labome.org)
  • Reduced dosage of ERF causes complex craniosynostosis in humans and mice and links ERK1/2 signaling to regulation of osteogenesis. (ox.ac.uk)
  • Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. (medlineplus.gov)
  • [email protected]#To study the clinical features and prognosis of core binding factor acute myeloid leukemia (CBF-AML) in children. (bvsalud.org)
  • A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. (bvsalud.org)
  • The MADS-box family of transcription factors. (ebi.ac.uk)
  • 2. A recombinant nucleic acid molecule comprising a promoter sequence operably linked to a nucleic acid molecule according to claim 1. (google.es)
  • Here we demonstrate that transforming growth factor β (TGFβ) is required for HSPC specification and that it regulates the expression of the Notch ligand Jagged1a in endothelial cells prior to EHT, in a striking parallel with the epithelial-to-mesenchymal transition (EMT). (ox.ac.uk)
  • RUNX3 suppresses gastric epithelial cell growth by inducing p21(WAF1/Cip1) expression in cooperation with transforming growth factor {beta}-activated SMAD. (springer.com)
  • Transcription factor Runx3 regulates interleukin-15-dependent natural killer cell activation. (springer.com)
  • RUNX3 inhibits the expression of vascular endothelial growth factor and reduces the angiogenesis, growth, and metastasis of human gastric cancer. (springer.com)
  • Cloning, mapping and expression of PEBP2 alpha C, a third gene encoding the mammalian Runt domain. (springer.com)
  • Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. (dartmouth.edu)
  • RUNX1T1 encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. (antibodies-online.com)
  • The presence of specific genetic mutations could aid clinicians in 4 ways: 1) diagnostic algorithms, 2) prognosis assessment, 3) use of targeted therapy, and 4) monitoring treatment response and residual disease. (thefreelibrary.com)
  • 4. A host cell comprising a recombinant nucleic acid molecule according to claim 2. (google.es)
  • Transcription regulator that excerts its function by binding to histone deacetylases and transcription factors. (abcam.com)
  • translocated to, 3 (CBFA2T3), transcript variant 2 with C terminal Flag and His tag. (trademetro.net)
  • These modifications in the intranuclear targeting of transcription factors might abrogate fidelity of gene expression in tumor cells by influencing the spatial organization and/or assembly of machineries involved in the synthesis and processing of gene transcripts. (umassmed.edu)
  • B) The TCR-stimulated cells were cultured in the presence of IL-2 for 5 d, washed with fresh media, and restimulated via TCR. (nih.gov)
  • In contrast, secretion of IFN-γ and IL-2 from the Runt-transgenic cells was decreased slightly and markedly, respectively, compared with the wild-type cells. (nih.gov)
  • The malignant cells were differentiated and considered mature cells after granulocyte-colony stimulating factor (G-CSF) treatment. (yonsei.ac.kr)
  • Thus, Txk is expressed on Th1/Th0 cells with the IFN-gamma production and acts as a Th1 cell-specific transcription factor. (labome.org)
  • FAS-L, IL-10, and double-negative CD4- CD8- TCR alpha/beta+ T cells are reliable markers of autoimmune lymphoproliferative syndrome (ALPS) associated with FAS loss of. (naver.com)
  • Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells. (ox.ac.uk)
  • EMDB-12611: RNA polymerase II core pre-initiation complex with closed promote. (pdbj.org)
  • Every molecule of RNA polymerase holoenzyme contains exactly one sigma factor subunit, which in the model bacterium Escherichia coli is one of those listed below. (wikipedia.org)
  • RNA polymerase holoenzyme complex consisting of core RNA polymerase and a sigma factor executes transcription of a DNA template strand. (wikipedia.org)
  • Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. (wikipedia.org)
  • Takeba Y, Nagafuchi H, Takeno M, Kashiwakura J, Suzuki N. Txk, a member of nonreceptor tyrosine kinase of Tec family, acts as a Th1 cell-specific transcription factor and regulates IFN-gamma gene transcription. (labome.org)
  • The transcription factor Gata3 has also been linked to haematological disease and was shown to promote HSC production in the embryo by inducing the secretion of important niche factors. (cam.ac.uk)
  • Kikuchi Y, Yasue T, Miyake K, Kimoto M, Takatsu K. CD38 ligation induces tyrosine phosphorylation of Bruton tyrosine kinase and enhanced expression of interleukin 5-receptor alpha chain: synergistic effects with interleukin 5. (labome.org)
  • These data indicate that CD38 ligation increases IL-5 receptor alpha expression and synergizes with IL-5 to enhance Blimp1 expression and IgM synthesis. (labome.org)
  • Transcription factor TFIIE alpha subunit, C-terminal / C-terminal general transcription factor TFIIE alpha / TFIIE beta central core DNA-binding domain profile. (pdbj.org)
  • Role of transforming growth factor beta in human disease. (springer.com)