Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.
A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.
A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.
A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.
A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.
A species of fruit fly much used in genetics because of the large size of its chromosomes.
Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.
Proteins prepared by recombinant DNA technology.
A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.
The largest of the TARSAL BONES which is situated at the lower and back part of the FOOT, forming the HEEL.
Autosomal dominant syndrome in which there is delayed closing of the CRANIAL FONTANELLES; complete or partial absence of the collarbones (CLAVICLES); wide PUBIC SYMPHYSIS; short middle phalanges of the fifth fingers; and dental and vertebral anomalies.
The amount of mineral per square centimeter of BONE. This is the definition used in clinical practice. Actual bone density would be expressed in grams per milliliter. It is most frequently measured by X-RAY ABSORPTIOMETRY or TOMOGRAPHY, X RAY COMPUTED. Bone density is an important predictor for OSTEOPOROSIS.
The back (or posterior) of the FOOT in PRIMATES, found behind the ANKLE and distal to the TOES.
A non-essential amino acid present abundantly throughout the body and is involved in many metabolic processes. It is synthesized from GLUTAMIC ACID and AMMONIA. It is the principal carrier of NITROGEN in the body and is an important energy source for many cells.
The constricted portion of the thigh bone between the femur head and the trochanters.
Reduction of bone mass without alteration in the composition of bone, leading to fractures. Primary osteoporosis can be of two major types: postmenopausal osteoporosis (OSTEOPOROSIS, POSTMENOPAUSAL) and age-related or senile osteoporosis.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.
Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
Hereditary disorder consisting of multiple basal cell carcinomas, odontogenic keratocysts, and multiple skeletal defects, e.g., frontal and temporoparietal bossing, bifurcated and splayed ribs, kyphoscoliosis, fusion of vertebrae, and cervicothoracic spina bifida. Genetic transmission is autosomal dominant.
A malignant skin neoplasm that seldom metastasizes but has potentialities for local invasion and destruction. Clinically it is divided into types: nodular, cicatricial, morphaic, and erythematoid (pagetoid). They develop on hair-bearing skin, most commonly on sun-exposed areas. Approximately 85% are found on the head and neck area and the remaining 15% on the trunk and limbs. (From DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1471)
Tumors or cancer of the SKIN.
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)
A form of pigmented nevus showing intense melanocytic activity around the dermo-epidermal junction. Large numbers of spindle-shaped melanocytes proliferate downward toward the dermis and usually a large amount of pigment is present. It was first described in 1976 and the bulk of patients reported have been young females with the lesions presenting on the thighs. (From Rook et al., Textbook of Dermatology, 4th ed, 1992, p185)
Tumors of cancer of the EYELIDS.
Antibodies produced by a single clone of cells.
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
Sites on an antigen that interact with specific antibodies.
Immunoglobulins produced in response to VIRAL ANTIGENS.

Cbfa1 isoforms exert functional differences in osteoblast differentiation. (1/863)

Cbfa1 is an essential transcription factor for osteoblast differentiation and bone formation. We investigated functional differences among three isoforms of Cbfa1: Type I (originally reported as Pebp2alphaA by Ogawa et al. (Ogawa, E., Maruyama, M., Kagoshima, H., Inuzuka, M., Lu, J., Satake, M., Shigesada, K., and Ito, Y. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 6859-6863), Type II (originally reported as til-1 by Stewart et al. (Stewart, M., Terry, A., Hu, M., O'Hara, M., Blyth, K., Baxter, E., Cameron, E., Onions, D. E., and Neil, J. C. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 8646-8651), and Type III (originally reported as Osf2/Cbfa1 by Ducy et al. (Ducy, P., Zhang, R., Geoffroy, V., Ridall, A. L., and Karsenty, G. (1997) Cell 89, 747-754). A reverse transcriptase-polymerase chain reaction analysis demonstrated that these isoforms were expressed in adult mouse bones. The transient transfection of Type I or Type II Cbfa1 in a mouse fibroblastic cell line, C3H10T1/2, induced the expression of alkaline phosphatase (ALP) activity. This induction was synergistically enhanced by the co-introduction of Xenopus BMP-4 cDNA. In contrast, the transient transfection of Type III cDNA induced no ALP activity. In C3H10T1/2 cells stably transfected with each isoform of Cbfa1, the gene expression of ALP was also strongly induced in cells transfected with Type I and Type II Cbfa1 but not in cells with Type III Cbfa1. Osteocalcin, osteopontin,and type I collagen gene expressions were induced or up-regulated in all of the cells stably transfected with each isoform of Cbfa1, and Type II transfected cells exhibited the highest expression level of osteocalcin gene. A luciferase reporter gene assay using a 6XOSE2-SV40 promoter (6 tandem binding elements for Cbfa1 ligated in front of the SV40 promoter sequence), a mouse osteocalcin promoter, and a mouse osteopontin promoter revealed the differences in the transcriptional induction of target genes by each Cbfa1 isoform with or without its beta-subunit. These results suggest that all three of the Cbfa1 isoforms used in the present study are involved in the stimulatory action of osteoblast differentiation, but they exert different functions in the process of osteoblast differentiation.  (+info)

Regulation of chondrocyte differentiation by Cbfa1. (2/863)

Cbfa1, a developmentally expressed transcription factor of the runt family, was recently shown to be essential for osteoblast differentiation. We have investigated the role of Cbfa1 in endochondral bone formation using Cbfa1-deficient mice. Histology and in situ hybridization with probes for indian hedgehog (Ihh), collagen type X and osteopontin performed at E13.5, E14.5 and E17.5 demonstrated a lack of hypertrophic chondrocytes in the anlagen of the humerus and the phalanges and a delayed onset of hypertrophy in radius/ulna in Cbfa1-/- mice. Detailed analysis of Cbfa1 expression using whole mount in situ hybridization and a lacZ reporter gene reveled strong expression not only in osteoblasts but also in pre-hypertrophic and hypertrophic chondrocytes. Our studies identify Cbfa1 as a major positive regulator of chondrocyte differentiation.  (+info)

Maturational disturbance of chondrocytes in Cbfa1-deficient mice. (3/863)

Cbfa1, a transcription factor that belongs to the runt-domain gene family, plays an essential role in osteogenesis. Cbfa1-deficient mice completely lacked both intramembranous and endochondral ossification, owing to the maturational arrest of osteoblasts, indicating that Cbfa1 has a fundamental role in osteoblast differentiation. However, Cbfa1 was also expressed in chondrocytes, and its expression was increased according to the maturation of chondrocytes. Terminal hypertrophic chondrocytes expressed Cbfa1 extensively. The significant expression of Cbfa1 in hypertrophic chondrocytes was first detected at embryonic day 13.5 (E13.5), and its expression in hypertrophic chondrocytes was most prominent at E14.5-16.5. In Cbfa1-deficient mice, whose entire skeleton was composed of cartilage, the chondrocyte differentiation was disturbed. Calcification of cartilage occurred in the restricted parts of skeletons, including tibia, fibula, radius, and ulna. Type X collagen, BMP6, and Indian hedgehog were expressed in their hypertrophic chondrocytes. However, osteopontin, bone sialoprotein, and collagenase 3 were not expressed at all, indicating that they are directly regulated by Cbfa1 in the terminal hypertrophic chondrocytes. Chondrocyte differentiation was severely disturbed in the rest of the skeleton. The expression of PTH/PTHrP receptor, Indian hedgehog, type X collagen, and BMP6 was not detected in humerus and femur, indicating that chondrocyte differentiation was blocked before prehypertrophic chondrocytes. These findings demonstrate that Cbfa1 is an important factor for chondrocyte differentiation.  (+info)

A Cbfa1-dependent genetic pathway controls bone formation beyond embryonic development. (4/863)

The molecular mechanisms controlling bone extracellular matrix (ECM) deposition by differentiated osteoblasts in postnatal life, called hereafter bone formation, are unknown. This contrasts with the growing knowledge about the genetic control of osteoblast differentiation during embryonic development. Cbfa1, a transcriptional activator of osteoblast differentiation during embryonic development, is also expressed in differentiated osteoblasts postnatally. The perinatal lethality occurring in Cbfa1-deficient mice has prevented so far the study of its function after birth. To determine if Cbfa1 plays a role during bone formation we generated transgenic mice overexpressing Cbfa1 DNA-binding domain (DeltaCbfa1) in differentiated osteoblasts only postnatally. DeltaCbfa1 has a higher affinity for DNA than Cbfa1 itself, has no transcriptional activity on its own, and can act in a dominant-negative manner in DNA cotransfection assays. DeltaCbfa1-expressing mice have a normal skeleton at birth but develop an osteopenic phenotype thereafter. Dynamic histomorphometric studies show that this phenotype is caused by a major decrease in the bone formation rate in the face of a normal number of osteoblasts thus indicating that once osteoblasts are differentiated Cbfa1 regulates their function. Molecular analyses reveal that the expression of the genes expressed in osteoblasts and encoding bone ECM proteins is nearly abolished in transgenic mice, and ex vivo assays demonstrated that DeltaCbfa1-expressing osteoblasts were less active than wild-type osteoblasts. We also show that Cbfa1 regulates positively the activity of its own promoter, which has the highest affinity Cbfa1-binding sites characterized. This study demonstrates that beyond its differentiation function Cbfa1 is the first transcriptional activator of bone formation identified to date and illustrates that developmentally important genes control physiological processes postnatally.  (+info)

Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation. (5/863)

Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene family that is expressed at high levels in diverse human carcinomas and in articular cartilage from arthritic patients. In addition to its expression in pathological conditions, collagenase 3 has been detected in osteoblasts and hypertrophic chondrocytes during fetal ossification. In this work, we have evaluated the possibility that Cbfa1 (core binding factor 1), a transcription factor playing a major role in the expression of osteoblastic specific genes, is involved in the expression of collagenase 3 during bone formation. We have functionally characterized a Cbfa motif present in the promoter region of collagenase 3 gene and demonstrated, by cotransfection experiments and gel mobility shift assays, that this element is involved in the inducibility of the collagenase 3 promoter by Cbfa1 in osteoblastic and chondrocytic cells. Furthermore, overexpression of Cbfa1 in osteoblastic cells unable to produce collagenase 3 leads to the expression of this gene after stimulation with transforming growth factor beta. Finally, we show that mutant mice deficient in Cbfa1, lacking mature osteoblasts but containing hypertrophic chondrocytes which are also a major source of collagenase 3, do not express this protease during fetal development. These results provide in vivo evidence that collagenase 3 is a target of the transcriptional activator Cbfa1 in these cells. On the basis of these transcriptional regulation studies, together with the potent proteolytic activity of collagenase 3 on diverse collagenous and noncollagenous bone and cartilage components, we proposed that this enzyme may play a key role in the process of bone formation and remodeling.  (+info)

Cbfa1 is required for epithelial-mesenchymal interactions regulating tooth development in mice. (6/863)

Osteoblasts and odontoblasts, cells that are responsible for the formation of bone and dentin matrices respectively, share several molecular characteristics. Recently, Cbfa1 was shown to be a critical transcriptional regulator of osteoblast differentiation. Mutations in this gene cause cleidocranial dysplasia (CCD), an autosomal dominant disorder in humans and mice characterized by defective bone formation. CCD also results in dental defects that include supernumerary teeth and delayed eruption of permanent dentition. The dental abnormalities in CCD suggest an important role for this molecule in the formation of dentition. Here we describe results of studies aimed at understanding the functions of Cbfa1 in tooth formation. RT-PCR and in situ hybridization analyses show that Cbfa1 has a unique expression pattern in dental mesenchyme from the bud to early bell stages during active epithelial morphogenesis. Unlike that observed in osteoblast differentiation, Cbfa1 is downregulated in fully differentiated odontoblasts and is surprisingly expressed in ectodermally derived ameloblasts during the maturation phase of enamel formation. The role of Cbfa1 in tooth morphogenesis is further illustrated by the misshapen and severely hypoplastic tooth organs in Cbfa1-/- mice. These tooth organs lacked overt odontoblast and ameloblast differentiation and normal dentin and enamel matrices. Epithelial-mesenchymal recombinants demonstrate that dental epithelium regulates mesenchymal Cbfa1 expression during the bud and cap stages and that these effects are mimicked by the FGFs but not by the BMPs as shown by our bead implantation assays. We propose that Cbfa1 regulates the expression of molecules in mesenchyme that act reciprocally on dental epithelium to control its growth and differentiation. Taken together, our data indicate a non-redundant role for Cbfa1 in tooth development that may be distinct from that in bone formation. In odontogenesis, Cbfa1 is not involved in the early signaling networks regulating tooth initiation and early morphogenesis but regulates key epithelial-mesenchymal interactions that control advancing morphogenesis and histodifferentiation of the epithelial enamel organ.  (+info)

Dexamethasone enhances In vitro vascular calcification by promoting osteoblastic differentiation of vascular smooth muscle cells. (7/863)

Vascular calcification is often associated with atherosclerotic lesions. Moreover, the process of atherosclerotic calcification has several features similar to the mineralization of skeletal tissue. Therefore, we hypothesized that vascular smooth muscle cells might acquire osteoblastic characteristics during the development of atherosclerotic lesions. In the present study, we investigated the effect of dexamethasone (Dex), which is well known to be a potent stimulator of osteoblastic differentiation in vitro, on vascular calcification by using an in vitro calcification model. We demonstrated that Dex increased bovine vascular smooth muscle cell (BVSMC) calcification in a dose- and time-dependent manner. Dex also enhanced several phenotypic markers of osteoblasts, such as alkaline phosphatase activity, procollagen type I carboxy-terminal peptide production, and cAMP responses to parathyroid hormone in BVSMCs. We also examined the effects of Dex on human osteoblast-like (Saos-2) cells and compared its effects on BVSMCs and Saos-2 cells. The effects of Dex on alkaline phosphatase activity and the cAMP response to parathyroid hormone in BVSMCs were less prominent than those in Saos-2 cells. Interestingly, we detected that Osf2/Cbfa1, a key transcription factor in osteoblastic differentiation, was expressed in both BVSMCs and Saos-2 cells and that Dex increased the gene expression of both transcription factors. These findings suggest that Dex may enhance osteoblastic differentiation of BVSMCs in vitro.  (+info)

Does adult fracture repair recapitulate embryonic skeletal formation? (8/863)

Bone formation is a continuous process that begins during fetal development and persists throughout life as a remodeling process. In the event of injury, bones heal by generating new bone rather than scar tissue; thus, it can accurately be described as a regenerative process. To elucidate the extent to which fetal skeletal development and skeletal regeneration are similar, we performed a series of detailed expression analyses using a number of genes that regulate key stages of endochondral ossification. They included genes in the indian hedgehog (ihh) and core binding factor 1 (cbfa1) pathways, and genes associated with extracellular matrix remodeling and vascular invasion including vascular endothelial growth factor (VEGF) and matrix metalloproteinase 13 (mmp13). Our analyses suggested that even at the earliest stages of mesenchymal cell condensation, chondrocyte (ihh, cbfa1 and collagen type II-positive) and perichondrial (gli1 and osteocalcin-positive) cell populations were already specified. As chondrocytes matured, they continued to express cbfa1 and ihh whereas cbfa1, osteocalcin and gli1 persisted in presumptive periosteal cells. Later, VEGF and mmp13 transcripts were abundant in chondrocytes as they underwent hypertrophy and terminal differentiation. Based on these expression patterns and available genetic data, we propose a model where Ihh and Cbfa1, together with Gli1 and Osteocalcin participate in establishing reciprocal signal site of injury. The persistence of cbfa1 and ihh, and their targets osteocalcin and gli1, in the callus suggests comparable processes of chondrocyte maturation and specification of a neo-perichondrium occur following injury. VEGF and mmp13 are expressed during the later stages of healing, coincident with the onset of vascularization of the callus and subsequent ossification. Taken together, these data suggest the genetic mechanisms regulating fetal skeletogenesis also regulate adult skeletal regeneration, and point to important regulators of angiogenesis and ossification in bone regeneration.  (+info)

TY - JOUR. T1 - Bone-specific transcription factor Runx2 interacts with the 1α,25-dihydroxyvitamin D3 receptor to up-regulate rat osteocalcin gene expression in osteoblastic cells. AU - Paredes, Roberto. AU - Arriagada, Gloria. AU - Cruzat, Fernando. AU - Villagra, Alejandro. AU - Olate, Juan. AU - Zaidi, Kaleem. AU - Van Wijnen, Andre. AU - Lian, Jane B.. AU - Stein, Gary S.. AU - Stein, Janet L.. AU - Montecino, Martin. PY - 2004/10. Y1 - 2004/10. N2 - Bone-specific transcription of the osteocalcin (OC) gene is regulated principally by the Runx2 transcription factor and is further stimulated in response to 1α,25-dihydroxyvitamin D3 via its specific receptor (VDR). The rat OC gene promoter contains three recognition sites for Runx2 (sites A, B, and C). Mutation of sites A and B, which flank the 1α,25-dihydroxyvitamin D3-responsive element (VDRE), abolishes 1α,25-dihydroxyvitamin D3-dependent enhancement of OC transcription, indicating a tight functional relationship between the VDR and ...
TY - JOUR. T1 - Loss of runt-related transcription factor 3 induces gemcitabine resistance in pancreatic cancer. AU - Horiguchi, Shigeru. AU - Shiraha, Hidenori. AU - Nagahara, Teruya. AU - Kataoka, Jyunnro. AU - Iwamuro, Masaya. AU - Matsubara, Minoru. AU - Nishina, Shinichi. AU - Kato, Hironari. AU - Takaki, Akinobu. AU - Nouso, Kazuhiro. AU - Tanaka, Takehiro. AU - Ichimura, Koichi. AU - Yagi, Takahito. AU - Yamamoto, Kazuhide. PY - 2013/8. Y1 - 2013/8. N2 - Background & Aim: Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene that is expressed in gastric and other cancers including pancreatic cancer. However, the precise function of RUNX3 in pancreatic cancer has not been fully elucidated. In this study, we aimed to determine the effect of decreased RUNX3 expression in pancreatic cancer. Methods: This study included 36 patients with primary pancreatic cancer, who had undergone pancreaticoduodenectomy. All patients were treated with 1000mg/m2 gemcitabine after the surgery. ...
Runt-related transcription factor 1 (RUNX1) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters and can accelerate apoptosis in various tumors. However, the regulatory mechanisms underlying RUNX1 expression in neuroblastoma (NB), a highly malignant tumor in childhood, remain largely unclear. In this study, we aimed to assess the role of RUNX1 in NB and to reveal the underlying mechanisms that may contribute to finding a potential therapeutics strategy against NB. Growth, invasion, metastasis and angiogenesis were assessed using Cell Counting Kit-8 (CCK-8) immunocytochemistry, and studies involving soft agar, cell invasion, tube formation and whole animals. The levels of expression were measured using real-time quantitative PCR for RNA, Western blot and immunostaining analyses for proteins. Luciferase reporter and chromatin immunoprecipitation assays indicated that RUNX1 directly binds within the BIRC5, CSF2RB and NFKBIA promoter regions to facilitate
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Objective: To evaluate the kinetics of bone formation of Pool 7 EMD, by concentration, on the amount of marrow formation and study factors relating to angiogenesis associated with EMD-induced bone formation. Methods: Fractionated commercial Emdogain® (EMD) was obtained from Straumann International. 60 CDI 7 day old outbred mice were injected using 50 μl aliquots of 10 µg EMD, 10 µg Pool 7 EMD, 2 µg TGF-β (positive control) or phosphate buffered saline (PBS, negative control) delivered in a calvarial injection model. Injections were done for 5 consecutive days. The animals were sacrificed on days 6, 8, 12, 16 and 21 with 6 animals per factor for each time point and were prepared for histological evaluation. Nikon NIS Elements software was used to analyze vascular growth and new bone formation over the five time intervals at 20x magnification. Immunocytochemistry (IHC) was used to identify the kinetics of osteogenesis by evaluating the osteoblast transcription factor Osterix, and the ...
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Our previous reports indicated that (+)-cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) by activating vitamin D receptor (VDR). However, whether and how miRNAs modulate osteogenic differentiation induced by (+)-cholesten-3-one have not been explored. In this study, miRNA array profiling and further validation by quantitative real-time PCR revealed that miR-351 was downregulated during (+)-cholesten-3-one-induced osteogenic differentiation of MSCs. Overexpression of miR-351 by miR-351 precursor transfection markedly inhibited the expression of osteoblast-specific genes, such as alkaline phosphatase (ALP), collagen type II, osteopontin (OPN), and runt-related transcription factor 2 (RUNX2), which consequently decreased a number of calcium mineralized nodules ...
van der Deen M, Taipaleenmaki H, Zhang Y, Teplyuk NM, Gupta A, Cinghu S, Shogren K, Maran A, Yaszemski MJ, Ling L, Cool SM, Leong DT, Dierkes C, Zustin J, Salto-Tellez M, Ito Y, Bae SC, Zielenska M, Squire JA, Lian JB, Stein JL, Zambetti GP, Jones SN, Galindo M, Hesse E, Stein GS, van Wijnen AJ. MicroRNA-34c inversely couples the biological functions of the runt-related transcription factor RUNX2 and the tumor suppressor p53 in osteosarcoma. J Biol Chem. 2013 Jul 19; 288(29):21307-19. Epub 2013 May 29 ...
Next-day shipping cDNA ORF clones derived from Runx1 runt-related transcription factor 1 available at GenScript, starting from $99.00.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
RUNX1 antibody (runt-related transcription factor 1) for ICC/IF, WB. Anti-RUNX1 pAb (GTX129100) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
RUNX1 antibody (runt-related transcription factor 1) for WB. Anti-RUNX1 pAb (GTX11903) is tested in Human, Mouse samples. 100% Ab-Assurance.
SOUTH SAN FRANCISCO, CA and THOUSAND OAKS, CA, May 26, 2009 (MARKET WIRE via COMTEX) -- Cytokinetics Incorporated (NASDAQ: CYTK) and Amgen Inc. (NASDAQ: AMGN) today announced that Amgen has exercised its option to obtain an exclusive license, worldwide (excluding Japan), to Cytokinetics cardiac contractility program. The license includes CK-1827452, a novel cardiac myosin activator being developed for the treatment of heart failure. Under the terms of the companies 2006 collaboration and option agreement, Amgen has agreed to pay Cytokinetics a non-refundable exercise fee of $50 million and has assumed responsibility for development and commercialization of CK-1827452 and related compounds, at its expense, subject to specified development and commercial participation rights of Cytokinetics. After reviewing the data from the CK-1827452 clinical trials, we are excited about the opportunity to advance this molecule, said Amgen Executive Vice President for Research and Development, Roger M. ...
The Author(s) 2014. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this conference please refer to the conferences website or contact the authors ...
Autor: Otto, Florian et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2002-02-13; Keywords: cleidocranial dysplasia; CCD; transcription factor; core binding factor; runt domain; RUNX2; CBFA1; differentiation; osteoblast; Titel: Mutations in the RUNX2 gene in patients with cleidocranial dysplasia
Cleidocranial dysplasia (CCD) is a rare autosomal dominant disorder mainly characterized by hypoplastic or absent clavicles, delayed closure of the fontanelles, multiple dental abnormalities, and short stature. Runt-related transcription factor 2 (RUNX2) gene variants can cause CCD, but are not identified in all CCD patients. In this study, we detected genetic variants in seven unrelated children with CCD by targeted high-throughput DNA sequencing or Sanger sequencing. All patients carried a RUNX2 variant, totally including three novel pathogenic variants (c.722_725delTGTT, p.Leu241Serfs*8; c.231_232delTG, Ala78Glyfs*82; c.909C | G, p.Tyr303*), three reported pathogenic variants (c.577C | T, p.Arg193*; c.574G | A, p.Gly192Arg; c.673 C | T, p.Arg225Trp), one likely pathogenic variant (c.668G | T, p.Gly223Val). The analysis of the variant source showed that all variants were de novo except the two variants (c.909C | G, p.Tyr303*; c.668G | T, p.Gly223Val) inherited from the patients father and mother with
The t(8;21) acute myeloid leukemia (AML)-associated oncoprotein AML1-ETO disrupts normal hematopoietic differentiation. Here, we have investigated its effects on the transcriptome and epigenome in t(8,21) patient cells. AML1-ETO binding was found at promoter regions of active genes with high levels of histone acetylation but also at distal elements characterized by low acetylation levels and binding of the hematopoietic transcription factors LYL1 and LMO2. In contrast, ERG, FLI1, TAL1, and RUNX1 bind at all AML1-ETO-occupied regulatory regions, including those of the AML1-ETO gene itself, suggesting their involvement in regulating AML1-ETO expression levels. While expression of AML1-ETO in myeloid differentiated induced pluripotent stem cells (iPSCs) induces leukemic characteristics, overexpression increases cell death. We find that expression of wild-type transcription factors RUNX1 and ERG in AML is required to prevent this oncogene overexpression. Together our results show that the interplay ...
BACKGROUND: Aberrant expression of the transcription factor RUNX2 in prostate cancer has a number of important consequences including increased resistance to apoptosis, invasion and metastasis to bone. We previously demonstrated that hypoxia up-regulated RUNX2 in tumour cells, which in turn up-regulated the anti-apoptotic factor Bcl-2. Here, we investigate the impact of nitric oxide (NO) on RUNX2 and Bcl-2 expression in prostate cancer and further, how RUNX2 over-expression can impact tumour growth, angiogenesis and oxygenation in vivo. METHODS: The effect of NO levels on RUNX2 and thus Bcl-2 expression was examined in prostate cancer cells in vitro using methods including gene and protein expression analyses, nitrite quantitation, protein-DNA interaction assays (ChIP) and viability assays (XTT). The effect of RUNX2 over-expression on tumour physiology (growth, oxygenation and angiogenesis) was also assessed in vivo using LNCaP xenografts. RESULTS: A low (but not high) concentration of NO (10microM)
The Runx2 transcription factor is critical for commitment to the osteoblast lineage. However, its role in committed osteoblasts and its functions during postnatal skeletogenesis remain unclear. We established a Runx2-floxed line with insertion of loxP sites around exon 8 of the Runx2 gene. Runx2 protein lacking the region encoded by exon 8 is imported into the nucleus and binds target DNA, but exhibits diminished transcriptional activity. We specifically deleted the Runx2 gene in committed osteoblasts using 2.3kb col1a-Cre transgenic mice. Surprisingly, the homozygous Runx2 mutant mice were born alive. The Runx2 heterozygous and homozygous null were grossly indistinguishable from wild-type littermates at birth. Runx2 deficiency did not alter proliferative capacity of osteoblasts during embryonic development (E18). Chondrocyte differentiation and cartilage growth in mutants was similar to wild-type mice from birth to 3 months of age. Analysis of the embryonic skeleton revealed poor calcification ...
PHF2라는 단백질이 뼈를 만드는 세포(조골세포)를 활성화시킨다는 사실을 처음으로 규명했다. 조골세포는 Runx2라는 단백질에 의해 분화가 조절된다. 반면, SUV39HI1라는 효소는 Runx2에 메틸기(CH3)를 붙임으로써 Runx2가 기능을 하지 못하게 하는 장식으로 분화를 방해한다. 성장이 끝난 성인들이 더 이상 키가 크지 않는 것도 SUV39HI1 효소 때문이다. 이에 착안해 Runx2에 붙어 있는 메틸기를 제거하는 방안을 연구한 결과, PHF2 단백질이 조골세포 분화를 유도함으로써, 소아의 뼈 발달 과정이나 골절 후 뼈가 새로 형성되는 과정에 작용한다는 것을 증명했다.. PHF2 단백질은 Runx2에 붙어 있는 메틸기를 제거했으며, 이후 본연의 기능을 회복한 Runx2는 조골세포의 분화를 촉진하여 다시 뼈를 만들기 시작했다. 실제 유전자 조작으로 PHF2 단백질이 과발현된 쥐를 만들어 ...
core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
Osteoblast differentiation is a pivotal event in bone formation. Runt-related transcription factor-2 (Runx2) is an essential factor required for osteoblast differentiation and bone formation. However, the underlying mechanism of Runx2-regulated osteogenic differentiation is still unclear. Here, we explored the corresponding mechanism using the C2C12/Runx2Dox subline, which expresses Runx2 in response to doxycycline (Dox). We found that Runx2-induced osteogenic differentiation of C2C12 cells results in a sustained decrease in the expression of heparin-binding EGF-like growth factor (HB-EGF), a member of the epidermal growth factor (EGF) family. Forced expression of HB-EGF or treatment with HB-EGF is capable of reducing the expression of alkaline phosphatase (ALP), a defined marker of early osteoblast differentiation. HB-EGF-mediated inhibition of ALP depends upon activation of the EGFR and the downstream extracellular signal-regulated kinase, c-Jun N-terminal kinase mitogen-activated protein kinase
Cleidocranial dysplasia (CCD) is an autosomal dominant skeletal dysplasia. Affected individuals have hypoplastic/aplastic clavicles and multiple dental abnormalities.
Osteoporosis results from the imbalance between bone resorption and bone formation, and restoring the normal balance of bone remodeling is highly desirable for identification of better treatment. In this study, using a cell-based high-throughput screening model representing Runt-related transcription factor 2 (RUNX2) transcriptional activity, we identified a novel small-molecular-weight compound, T63, as an efficient up-regulator of osteogenesis. T63 increased the alkaline phosphatase (ALPL) activity and mineralization as well as gene expression of Alpl and other osteogenic marker genes in mouse osteoblasts and mesenchymal stem cell-like cells. Upon induction of osteoblast differentiation, T63 inhibited adipogenic differentiation in the pluripotent mesenchymal cells. Consistently, T63 up-regulated RUNX2 mRNA and protein levels, and knockdown of RUNX2 reduced the osteogenic role of T63. Mechanistically, T63 activated both BMPs and WNT/β-catenin signaling pathways. Inhibition of either signaling pathway
Runt-related transcription factor 1 (Runx1), a master regulator of hematopoiesis, is expressed in preosteoclasts. Previously we evaluated the bone phenotype of CD11b-Cre Runx1(fl/fl) mice and demonstrated enhanced osteoclasts and decreased bone mass in males. However, an assessment of the effects of Runx1 deletion in female osteoclast precursors was impossible with this model. Moreover, the role of Runx1 in myeloid cell differentiation into other lineages is unknown. Therefore, we generated LysM-Cre Runx1(fl/fl) mice, which delete Runx1 equally (∼80% deletion) in myeloid precursor cells from both sexes and examined the capacity of these cells to differentiate into osteoclasts and phagocytic and antigen-presenting cells. Both female and male LysM-Cre Runx1(fl/fl) mice had decreased trabecular bone mass (72% decrease in bone volume fraction) and increased osteoclast number (2-3 times) (P < .05) without alteration of osteoblast histomorphometric indices. We also demonstrated that loss of Runx1 in ...
TY - JOUR. T1 - Tyrosine phosphorylation controls Runx2-mediated subnuclear targeting of YAP to repress transcription. AU - Zaidi, Sayyed K.. AU - Sullivan, Andrew J.. AU - Medina, Ricardo. AU - Ito, Yoshiaki. AU - van Wijnen, Andre J. AU - Stein, Janet L.. AU - Lian, Jane B.. AU - Stein, Gary S.. PY - 2004/2/25. Y1 - 2004/2/25. N2 - Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes-associated protein (YAP), a mediator of Src/Yes signaling, interacts with the native Runx2 protein, an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. Runx2, through its PY motif, recruits YAP to subnuclear domains in situ and to the osteocalcin (OC) gene promoter in vivo. Inhibition of Src/Yes kinase blocks tyrosine phosphorylation of YAP and dissociates endogenous Runx2-YAP complexes. Consequently, recruitment of the YAP co-repressor to ...
2018 The Author(s) Oncogenic transcription factors such as the leukemic fusion protein RUNX1/ETO, which drives t(8;21) acute myeloid leukemia (AML), constitute cancer-specific but highly challenging therapeutic targets. We used epigenomic profiling data for an RNAi screen to interrogate the transcriptional network maintaining t(8;21) AML. This strategy identified Cyclin D2 (CCND2) as a crucial transmitter of RUNX1/ETO-driven leukemic propagation. RUNX1/ETO cooperates with AP-1 to drive CCND2 expression. Knockdown or pharmacological inhibition of CCND2 by an approved drug significantly impairs leukemic expansion of patient-derived AML cells and engraftment in immunodeficient murine hosts. Our data demonstrate that RUNX1/ETO maintains leukemia by promoting cell cycle progression and identifies G1 CCND-CDK complexes as promising therapeutic targets for treatment of RUNX1/ETO-driven AML. Using in vitro and in vivo screens to identify essential RUNX1/ETO transcriptional targets in AML, Martinez-Soria ...
The molecular mechanisms that transduce the osteoblast response to physical forces in the bone microenvironment are poorly understood. Here, we used genetic and pharmacological experiments to determine whether the polycystins PC1 and PC2 (encoded by Pkd1 and Pkd2) and the transcriptional coactivator TAZ form a mechanosensing complex in osteoblasts. Compound-heterozygous mice lacking 1 copy of Pkd1 and Taz exhibited additive decrements in bone mass, impaired osteoblast-mediated bone formation, and enhanced bone marrow fat accumulation. Bone marrow stromal cells and osteoblasts derived from these mice showed impaired osteoblastogenesis and enhanced adipogenesis. Increased extracellular matrix stiffness and application of mechanical stretch to multipotent mesenchymal cells stimulated the nuclear translocation of the PC1 C-terminal tail/TAZ (PC1-CTT/TAZ) complex, leading to increased runt-related transcription factor 2-mediated (Runx2-mediated) osteogenic and decreased PPARγ-dependent adipogenic ...
Sometimes, a lot of people discover the same protein and so it gets a lot of names, like Runt-related transcription factor 2 (Runx2), which is also known as: Cbfa1, Osf2, AML3, Til1, and Pebp2 αA or PPB2αA… talk about having an identity crisis!. After writing my list, I Googled funny gene names to see what other funny names are out their and discovered on this site that there are waaaaaaay better gene names that Ive never heard of before (probably the best on this list are cheap date, lunatic fringe, grim and reaper (which work together to control cell death), and INDY, which makes mutant fruit flies live twice as long… its an acronym for Im not dead yet (from Monty Python and the Holy Grail).. ...
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Loss of RUNX1/ETO Triggers C/EBPα-Driven Reorganization of the Leukemic Transcriptional Network(A) RUNX1/ETO and CEBPA mRNA expression levels in Kasumi-1 cells
CATAGAGCCA GCGGGCGCGG GCGGGACGGG CGCCCCGCGG CCGGACCCAG CCAGGGCACC ACGCTGCCCG GCCCTGCGCC GCCAGGCACT TCTTTCCGGG ^1 ^11 ^21 ^31 ^41 ^51 ^61 ^71 ^81 ^91 GCTCCTAGGG ACGCCAGAAG GAAGTCAACC TCTGCTGCTT CTCCTTGGCC TGCGTTGGAC CTTCCTTTTT TTGTTGTTTT TTTTTGTTTT TCCCCTTTCT ^101 ^111 ^121 ^131 ^141 ^151 ^161 ^171 ^181 ^191 TCCTTTTGAA TTAACTGGCT TCTTGGCTGG ATGTTTTCAA CTTCTTTCCT GGCTGCGAAC TTTTCCCCAA TTGTTTTCCT TTTACAACAG GGGGAGAAAG ^201 ^211 ^221 ^231 ^241 ^251 ^261 ^271 ^281 ^291 TGCTCTGTGG TCCGAGGCGA GCCGTGAAGT TGCGTGTGCG TGGCAGTGTG CGTGGCAGGA TGTGCGTGCG TGTGTAACCC GAGCCGCCCG ATCTGTTTCG ^301 ^311 ^321 ^331 ^341 ^351 ^361 ^371 ^381 ^391 ATCTGCGCCG CGGAGCCCTC CCTCAAGGCC CGCTCCACCT GCTGCGGTTA CGCGGCGCTC GTGGGTGTTC GTGCCTCGGA GCAGCTAACC GGCGGGTGCT ^401 ^411 ^421 ^431 ^441 ^451 ^461 ^471 ^481 ^491 GGGCGACGGT GGAGGAGTAT CGTCTCGCTG CTGCCCGAGT CAGGGCTGAG TCACCCAGCT GATGTAGACA GTGGCTGCCT TCCGAAGAGT GCGTGTTTGC ^501 ^511 ^521 ^531 ^541 ^551 ^561 ^571 ^581 ^591 ATGTGTGTGA CTCTGCGGCT GCTCAACTCC CAACAAACCA GAGGACCAGC ...
TY - JOUR. T1 - Uremia induces the osteoblast differentiation factor Cbfa1 in human blood vessels. AU - Moe, Sharon. AU - Duan, Danxia. AU - Doehle, Brian P.. AU - ONeill, Kalisha D.. AU - Chen, Xuening (Neal). PY - 2003/3/1. Y1 - 2003/3/1. N2 - Background. Bone matrix proteins are expressed in calcified arteries from dialysis patients, suggesting that vascular smooth muscle cells (VSMCs) may transform to osteoblast-like cells. One of the key transcriptional regulators of osteoblast differentiation is Cbfa1. Thus, we hypothesized that this may be a key factor in arterial calcification. Methods. To test this hypothesis, we examined sections of the inferior epigastric artery from uremic patients for the presence of Cbfa1 and type I collagen and osteopontin by in situ hybridization and immunostaining. We also examined the effect of pooled uremic sera from dialysis patients on the expression of Cbfa1 by reverse transcription-polymerase chain reaction (RT-PCR) in bovine VSMCs in vitro. Results. ...
Looking for online definition of cleidocranial in the Medical Dictionary? cleidocranial explanation free. What is cleidocranial? Meaning of cleidocranial medical term. What does cleidocranial mean?
Cleidocranial dysplasia (CCD) is a autosomal dominant disorder characterized by skeletal anomalies such as patent fontanels, late closure of cranial sutures with Wormian bones, late erupting secondary dentition, rudimentary clavicles, and short stature. The locus for this disease was mapped to chrom …
Osteoporosis is a complex multifactorial disorder of the skeleton. Genetic factors are important in determining peak bone mass and structure, as well as the predisposition to bone deterioration and fragility fractures. Nonetheless, genetic factors alone are not sufficient to explain osteoporosis development and fragility fracture occurrence. Indeed, epigenetic factors, representing a link between individual genetic aspects and environmental influences, are also strongly suspected to be involved in bone biology and osteoporosis. Recently, alterations in epigenetic mechanisms and their activity have been associated with aging. Also, bone metabolism has been demonstrated to be under the control of epigenetic mechanisms. Runt-related transcription factor 2 (RUNX2), the master transcription factor of osteoblast differentiation, has been shown to be regulated by histone deacetylases and microRNAs (miRNAs). Some miRNAs were also proven to have key roles in the regulation of Wnt signalling in osteoblastogenesis
Currently, no model is available to study the cellular and molecular events associated with bone metastases of prostate cancer. This study shows that MDA PCa 2a and MDA PCa 2b cells induce a specific and reproducible increase in osteoblast differentiation and proliferation when the cells share the medium during coculturing. Osteoblast differentiation in this system was associated with up-regulation of the osteoblast-specific transcriptor factor Cbfa1. Moreover, up-regulation of Cbfa1 and Osteocalcin expression was also induced in PMOs by CM produced by MDA PCa 2b cells, suggesting that soluble factors produced by prostate cancer cells promote osteoblast differentiation and that Cbfa1 mediates this effect. To our knowledge, this is the first in vitro model of bone metastasis from prostate cancer that recapitulates the osteoblastic phenotype typical of the disease. These results confirmed in vivo and at the molecular level, suggest that the pathophysiology of osteoblastic bone metastases from ...
Gla-osteocalcin and Glu-osteocalcin can be assayed concurrently with the Mouse Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK127) and Mouse Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK129), respectively. These mouse osteocalcin ELISA kits allow for the relative evaluation of Gla/Glu-osteocalcins, thereby providing a measure of both bone formation and bone resorption.. These kits use a sandwich ELISA strategy to specifically detect the Gla and Glu forms of osteocalcin. The capture antibody is a plate-bound solid-phased rat monoclonal antibody that specifically recognizes the C-terminal region of mouse osteocalcin. This is paired with labeled monoclonal antibodies that are specific for captured Glu-type or Gla-type osteocalcin. Because mouse osteocalcin has C-terminal sequences that differ from other mammals, it is possible to measure mouse osteocalcin without any cross-reaction with bovine antigens. Therefore, the process of osteoblastic cell differentiation can be monitored in ...
Gla-osteocalcin and Glu-osteocalcin can be assayed concurrently with the Mouse Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK127) and Mouse Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK129), respectively. These mouse osteocalcin ELISA kits allow for the relative evaluation of Gla/Glu-osteocalcins, thereby providing a measure of both bone formation and bone resorption.. These kits use a sandwich ELISA strategy to specifically detect the Gla and Glu forms of osteocalcin. The capture antibody is a plate-bound solid-phased rat monoclonal antibody that specifically recognizes the C-terminal region of mouse osteocalcin. This is paired with labeled monoclonal antibodies that are specific for captured Glu-type or Gla-type osteocalcin. Because mouse osteocalcin has C-terminal sequences that differ from other mammals, it is possible to measure mouse osteocalcin without any cross-reaction with bovine antigens. Therefore, the process of osteoblastic cell differentiation can be monitored in ...
Work from Dr. Rafiis laboratory, among others, has revealed the heterogeneity of endothelial cells, which comprise over 140 different types of endothelium in the human body. Each organ or tumor is vascularized by a specialized endothelium. It is believed that transcription factors belonging to the Ets family, such as Ets variant 2 (ETV2), Fli1 and the Ets-related gene (Erg), make endothelial cells organ-specific. Endothelial cells are important niche cells for hematopoietic stem cells (HSC) and their use as feeder cells in culture allows the expansion of HSC by ~150-fold.1 As a refinement, a combination of reprogramming factors, including FBJ murine osteosarcoma viral oncogene homolog B (FOSB), growth factor independent 1 transcriptional repressor (GFI1), runt-related transcription factor 1 (RUNX1) and SPI1 (which encodes PU.1), can be combined with sustained vascular niche induction to generate HSC that are endowed with secondary repopulating activity.. However, a maladapted vascular niche can ...
Osterix, a zinc-finger transcription factor, is required for osteoblast differentiation and new bone formation during embryonic development. The c-Src of tyrosine kinase is involved in a variety of cellular signaling pathways, leading to the induction of DNA synthesis, cell proliferation, and cytoskeletal reorganization. Src activity is tightly regulated and its dysregulation leads to constitutive activation and cellular transformation. The function of Osterix can be also modulated by post-translational modification. But the precise molecular signaling mechanisms between Osterix and c-Src are not known. In this study we investigated the potential regulation of Osterix function by c-Src in osteoblast differentiation. We found that c-Src activation increases protein stability, osteogenic activity and transcriptional activity of Osterix. The siRNA-mediated knockdown of c-Src decreased the protein levels and transcriptional activity of Osterix. Conversely, Src specific inhibitor, SU6656, decreased ...
Breast cancer is one of the most common malignant diseases in women. Epithelial-mesenchymal transition (EMT) has been documented to play an important role in proliferation, invasion and metastasis of tumor cells as well as drug resistance. Even though the signal transducer and activator of transcription 3 (STAT3) is not a master transcription factor of EMT, STAT3 is involved in the regulation of EMT-related gene expression. However, it remains unclear whether targeted inhibitors of STAT3 affect EMT-mediated proliferation, migration, invasion and drug resistance of tumor cells. In this paper, we investigated the effects of STAT3 and its interaction with Twist, a master transcription factor, in EMT program and subsequent changes in proliferation, migration and invasion of breast cancer cells by interfering STAT3 signaling pathway with different strategies such as STAT3 inactivation and STAT3 silencing. Furthermore, we explored the role of inhibiting STAT3 phosphorylation in the EMT regulation of ...
c-Src and IL-6 inhibit osteoblast differentiation and integrate IGFBP5 signalling.: Interleukin-6 (IL-6) and c-Src impair osteoblast maturation in vitro and in
FOXP3+CD4+ regulatory T cells (Tregs) are critical for immune homeostasis and respond to local tissue cues, which control their stability and function. We explored here whether developmental endothelial locus-1 (DEL-1), which, like Tregs, increases during resolution of inflammation, promotes Treg responses. DEL-1 enhanced Treg numbers and function at barrier sites (oral and lung mucosa). The underlying mechanism was dissected using mice lacking DEL-1 or expressing a point mutant thereof, or mice with T cell-specific deletion of the transcription factor RUNX1, identified by RNA sequencing analysis of the DEL-1-induced Treg transcriptome. Specifically, through interaction with αvβ3 integrin, DEL-1 promoted induction of RUNX1-dependent FOXP3 expression and conferred stability of FOXP3 expression upon Treg restimulation in the absence of exogenous TGF-β1. Consistently, DEL-1 enhanced the demethylation of the Treg-specific demethylated region (TSDR) in the mouse Foxp3 gene and the suppressive ...
BioAssay record AID 638977 submitted by ChEMBL: Induction of osteoblast differentiation in mouse C2C12 cells assessed as increase in ALP promoter activity measured 48 hrs by luciferase reporter gene analysis.
Engineering surfaces to direct integrin binding and signaling to promote osteoblast differentiation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Mouse Monoclonal Anti-RUNX3/CBFA3 Antibody (2B10E8) [DyLight 488]. Validated: WB, Flow, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
Xp3 (panel A), GZMB (panel B), RUNX3 (panel C) immunostaining. *P,0,05 vs NM; { P,0,05 vs CRC. RUNX3 level in CD8+ T cells coexpressing (white bars) and not
Osteoblast differentiation requires the coordinated stepwise expression of multiple genes. Histone deacetylase inhibitors (HDIs) accelerate the osteoblast differentiation process by blocking the activity of histone deacetylases (HDACs), which alter gene expression by modifying chromatin structure. We previously demonstrated that HDIs and HDAC3 shRNAs accelerate matrix mineralization and the expression of osteoblast maturation genes (e.g. alkaline phosphatase, osteocalcin). Identifying other genes that are differentially regulated by HDIs might identify new pathways that contribute to osteoblast differentiation. To identify other osteoblast genes that are altered early by HDIs, we incubated MC3T3-E1 preosteoblasts with HDIs (trichostatin A, MS-275, or valproic acid) for 18 hours in osteogenic conditions. The promotion of osteoblast differentiation by HDIs in this experiment was confirmed by osteogenic assays. Gene expression profiles relative to vehicle-treated cells were assessed by microarray analysis
Effects of silica-gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells Wei He,1 Dina A Mosselhy,2,3 Yudong Zheng,1 Qingling Feng,4 Xiaoning Li,4 Xing Yang,4 Lina Yue,1 Simo-Pekka Hannula2 1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, Peopleâ s Republic of China; 2Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Espoo, Finland; 3Microbiological Unit, Fish Diseases Department, Animal Health Research Institute, Giza, Egypt; 4State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, Peopleâ s Republic of China Introduction: In recent years, there has been an increasing interest in silica (SiO2) nanoparticles (NPs) as drug delivery systems. This interest is mainly attributed to the ease of their surface functionalization for drug loading. In orthopedic applications, gentamicin-loaded SiO2
The heterotopic ossification of muscles, tendons, and ligaments is a common problem faced by orthopaedic surgeons. Runx2/Cbfa1 plays an essential role during the osteoblast differentiation and is considered as a molecular switch in osteoblast biology. RNA interference technology is a powerful tool for silencing endogenous or exogenous genes in mammalian cells. In this study, we investigated the effect of Runx2/Cbfa1-specific siRNA on osteoblast differentiation and mineralization in osteoblastic cells, and then constructed adenovirus containing siRNA against Runx2/Cbfa1 (Ad-Runx2-siRNA) to inhibit the formation of heterotopic ossification induced by BMP4, dernineralized bone matrix, and trauma in animal model. Our results showed that the Runx2/Cbfa1-specific siRNA could inhibit the expression of Runx2/Cbfa1 at the level of mRNA and protein. Analysis of the expression of osteoblast maturation genes including type I collagen, osteopontin, bone sialoprotein, and osteocalcin, alkaline phosphatase ...
Genetic studies show that Msx2 and Dlx5 homeodomain (HD) proteins support skeletal development, but null mutation of the closely related Dlx3 gene results in early embryonic lethality. Here we find that expression of Dlx3 in the mouse embryo is associated with new bone formation and regulation of osteoblast differentiation. Dlx3 is expressed in osteoblasts, and overexpression of Dlx3 in osteoprogenitor cells promotes, while specific knock-down of Dlx3 by RNA interference inhibits, induction of osteogenic markers. We characterized gene regulation by Dlx3 in relation to that of Msx2 and Dlx5 during osteoblast differentiation. Chromatin immunoprecipitation assays revealed a molecular switch in HD protein association with the bone-specific osteocalcin (OC) gene. The transcriptionally repressed OC gene was occupied by Msx2 in proliferating osteoblasts, while Dlx3, Dlx5, and Runx2 were recruited postproliferatively to initiate transcription. Dlx5 occupancy increased over Dlx3 in mature osteoblasts at the
Recent research has uncovered complex transcription factor networks that control the processes of T-cell development and differentiation. RUNX (runt-related transcription factor) proteins are among the many factors that have crucial roles in these networks. In this Review, we examine the mechanisms …
The Runt related transcription factors (RUNX) are recognized as key players in suppressing or promoting tumor growth. RUNX3, a member of this family, is known as a tumor suppressor in many types of cancers, although such a paradigm was challenged by some researchers. The TGF-β pathway governs major upstream signals to activate RUNX3. RUNX3 protein consists of several regions and domains. The Runt domain is a conserved DNA binding domain and is considered as the main part of RUNX proteins since. Herein, we compared the effects of Runt domains and full-Runx3 in cell viability by designing two constructs of Runx3, including N-terminal region and Runt domain. We investigated the effect of full-Runx3, N-t, and RD on growth inhibition in AGS, MCF-7, A549, and HEK293 cell lines which are different in TGF-β sensitivity, in the absence and presence of TGF-β. The full length RUNX3 did not notably inhibit growth of these cell lines while, the N-t and RD truncates showed different trends in these cell lines.
Cell culture experiments have indicated that TGF-β and BMPs have both overlapping and opposing functions in bone formation. On the one hand, TGF-β stimulates the recruitment and proliferation of osteoblast progenitors but inhibits their terminal differentiation; and on the other hand, BMPs cooperate in the former process in addition to promoting osteogenic commitment of MSCs and osteoblast maturation (Alliston et al., 2008). However, albeit informative, these in vitro analyses have used exogenous stimulators or inhibitors of cell signaling and differentiation to infer the dynamics of locally released TGF-β and BMP signals during bone formation. For example, increased differentiation of C2C12 cells treated with BMP4 in the presence of an ALK5 inhibitor was interpreted to imply that endogenous TGF-β activity maintains normal bone mass by restricting the rate of osteoblast maturation through Smad-directed blockade of BMP signaling (Maeda et al., 2004). Similarly, genetic studies in mice have ...
Cellular identity in metazoan organisms is frequently established through lineage-specifying transcription factors, which control their own expression through transcriptional positive feedback, while antagonizing the developmental networks of competing lineages. Here, we have uncovered a distinct positive feedback loop that arises from the reciprocal stabilization of the tyrosine kinase ABL and the transcriptional coactivator TAZ. Moreover, we determined that this loop is required for osteoblast differentiation and embryonic skeletal formation. ABL potentiated the assembly and activation of the RUNX2-TAZ master transcription factor complex that is required for osteoblastogenesis, while antagonizing PPARγ-mediated adipogenesis. ABL also enhanced TAZ nuclear localization and the formation of the TAZ-TEAD complex that is required for osteoblast expansion. Last, we have provided genetic data showing that regulation of the ABL-TAZ amplification loop lies downstream of the adaptor protein 3BP2, which ...
The researchers were surprised to discover that this enhanced mineralization was not regulated by the transcription factor RUNX2, which is usually associated with this process, but was instead regulated by TNAP via the transcription factor RARβ. Further in vitro investigations revealed that unfolded HLA-B27 causes the upregulation of TNAP in AS-derived MSCs via the IRE1α-sXBP1 pathway of the unfolded protein response. Crucially, serum concentrations of bone-specific TNAP were increased in patients with AS compared with healthy individuals from two different cohorts and correlated with disease progression. ...
The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
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goals inhibiting Runx1 print high download Will We of coordinate and small retardation( Chen CL et al. Runx1 is released to refer the colorectal bradykinin of fungal adult diagram phosphatidylinositol genes during homogeneous activity Meanwhile through Activation of binding transporter( Kobayashi et al. In phosphorylase and mismatch levels, Runx1 purine forms inhibited to the electronic transcript of the transcriptional heart methionine, to advantageous specific linear isoforms. methylmalonic homo- in activity and loading cells leads been to chemical target of the Sustainable virus fusion, to phosphorylated 3T3 levels( Chen AI et al. RUNX1 recessed 1-diphosphate of not termed beauties will listen known when central studies rise catalytic. RUNX2( CBFA1 or AML3) potential cascade, immune to In-frame RUNX T cells, RUNX1 and RUNX3, can activate in heptapeptide with CBFB( CBF-beta)( Kundu et al. RUNX2 already has work of cells stimulated in new reaction( implicated in Karsenty 2008).
Col10a1 promoter activity is up-regulated via RUNX2 binding elements in vitro. (A) Transactivation of Col10a1 via RUNX2-binding A and B elements. The RUNX2 expr
Mouse Monoclonal Anti-RUNX3/CBFA3 Antibody (2B10E8) [Biotin]. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
Expression of RUNX3 (AML2, CBFA3, PEBP2A3) in vagina tissue. Antibody staining with HPA059006 and CAB025416 in immunohistochemistry.
DLX3 is a novel target of PRKACA, and PRKACA mediates BMP signaling during osteoblast differentiation, at least in part, by phosphorylating DLX3 and modulating the protein stability and function of DLX3 ...
Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that ... The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants ... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for ... The phosphorylation state of Runx2 also mediates its DNA-binding activity. The Runx2 DNA-binding activity is correlated with ...
... core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5'-pygpyggt-3', of a number of enhancers and ... The protein is a heterodimer of alpha- and beta-subunits. The alpha-subunit binds DNA as a monomer, and appears to have a role ... highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit ... The region of shared similarity, known as the Runt domain, is responsible for DNA-binding and protein-protein interaction. In ...
... or core-binding factor subunit alpha-2 (CBFA2) is a protein that in humans is encoded by the RUNX1 gene. RUNX1 is a ... a DNA binding CBFα chain (RUNX1 or RUNX2) and a non-DNA-binding subunit called core binding factor β (CBFβ); the binding ... Wang, S, Speck, NA (January 1992). "Purification of core-binding factor, a protein that binds the conserved core site in murine ... It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). ...
Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. It was previously believed that the RNA ... The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) ... Sigma factors in E. coli:. *σ70(RpoD) - σA - the "housekeeping" sigma factor or also called as primary sigma factor, ...
... later showed that the sigma factor does not obligatorily leave the core. Instead, it changes its binding with the core during ... subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. It was previously believed that the RNA ... The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) ... Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ...
This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several ... The protein complex that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to ... "Induced alpha helix in the VP16 activation domain upon binding to a human TAF". Science. 277 (5330): 1310-3. doi:10.1126/ ... TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa, also known as TAF9, is a protein that in ...
Chen Y; Le Cahérec F; Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. GRCh38: ... Knight BC; High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ...
DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-13. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... "New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ...
The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ... Transcription initiation factor TFIID subunit 11 also known as TAFII28, is a protein that in humans is encoded by the TAF11 ... The conserved region contains four alpha helices and three loops arranged as in histone H3. TAF11 has been shown to interact ... In molecular biology, TAFII28 refers to the TATA box binding protein associated factor. Together with the TATA-binding protein ...
... it allosterically enhances DNA binding by the alpha subunit as the complex binds to the core site of various enhancers and ... Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family ...
TATA box-binding protein-associated factor) subunits (TAF1A, TAF1B, and TAF1C). These TAFs don't have a histone-like fold ... It contains two short alpha helices and a long central alpha helix. TAF1 (TAFII250) TAF2 (CIF150) TAF3 (TAFII140) TAF4 ( ... for example the downstream promoter element or gene-specific core promoter sequence Due to such interactions, they contribute ... The TBP-associated factors (TAF) are proteins that associate with the TATA-binding protein in transcription initiation. It is a ...
... subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, ... RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa, a beta (β) ... The core enzyme has five subunits (~400 kDa):[23] *β': The β' subunit is the largest subunit, and is encoded by the rpoC gene.[ ... In order to bind promoters, RNAP core associates with the transcription initiation factor sigma (σ) to form RNA polymerase ...
This contains a core of two compact domains with each having five alpha helices. The first five-helix bundle is a conserved ... Viral cyclin D binds human Cdk6 and inhibits Rb by phosphorylating it, resulting in free transcription factors which result in ... A simplification in yeast is that all cyclins bind to the same Cdc subunit, the Cdc28. Cyclins in yeast are controlled by ... A role for cAMP response element-binding protein and activating transcription factor-2 in pp60(v-src) signaling in breast ...
"The alpha-like RNA polymerase II core subunit 3 (RPB3) is involved in tissue-specific transcription and muscle differentiation ... cooperation with promoter-bound activator domains and binding to TFIIB". J. Mol. Biol. 261 (5): 599-606. doi:10.1006/jmbi. ... POLR2J has been shown to interact with: Apoptosis antagonizing transcription factor, POLR2C, and SATB1. GRCh38: Ensembl release ... The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of ...
This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 ... Chen Y, Le Cahérec F, Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... Knight BC, High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...
It belongs to the thioredoxin superfamily fold which is defined by a beta-sheet core surrounded by alpha-helices. The active ... This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite ... Li S, Rosen BP, Borges-Walmsley MI, Walmsley AR (July 2002). "Evidence for cooperativity between the four binding sites of ... The arsC family also comprises the Spx proteins which are Gram-positive bacterial transcription factors that regulate the ...
"Multidomain organization of eukaryotic guanine nucleotide exchange translation initiation factor eIF-2B subunits revealed by ... The structure can be divided into a structural C-terminal core onto which the two N-terminal helices are attached. The core ... The W2 domain has a globular fold and is exclusively composed out of alpha-helices. ... the eIF-W2 domain functions as the binding site for Mnk eIF4E kinase, an enzyme that phosphorylates eukaryotic initiation ...
In normoxia, HIF alpha subunits are marked for the ubiquitin-proteasome degradation pathway through hydroxylation of proline- ... "Structural basis for binding of hypoxia-inducible factor to the oxygen-sensing prolyl hydroxylases". Structure. 17 (7): 981-9. ... The catalytic domain consists of a double-stranded β-helix core that is stabilized by three α-helices packed along the major β- ... X-ray crystallography and NMR spectroscopy showed that both peptides bind to the same binding site on PHD2, in a cleft on the ...
The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ... "Structure-function analysis of the estrogen receptor alpha corepressor scaffold attachment factor-B1: identification of a ... This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and ... TATA-binding protein-associated factor 2N is a protein that in humans is encoded by the TAF15 gene. Initiation of transcription ...
The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are ... It also binds closely to the E3 ubiquitin ligase MDM2, which is a regulator of the degradation of p53 and retinoblastoma ... Accordingly, gene expression by degradation of transcription factors, such as p53, c-jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, ... which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
Ruediger R, Fields K, Walter G (1999). "Binding specificity of protein phosphatase 2A core enzyme for regulatory B subunits and ... 1990). "alpha- and beta-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure ... Hong Y, Sarge KD (1999). "Regulation of protein phosphatase 2A activity by heat shock transcription factor 2". J. Biol. Chem. ... It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, ...
"BRCA1 augments transcription by the NF-kappaB transcription factor by binding to the Rel domain of the p65/RelA subunit". The ... Karetsou Z, Kretsovali A, Murphy C, Tsolas O, Papamarcaki T (April 2002). "Prothymosin alpha interacts with the CREB-binding ... "Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) ... "DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in ...
"The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative ... which interacts with the seven-membered alpha ring of 20S core particle and eastablishs an asymmetric interface between the 19S ... It also have subunits that can bind with nucleotides (e.g., ATPs) in order to facilitate the association between 19S and 20S ... These subunits can be categorized into two classes based on the ATP dependence of subunits, ATP-dependent subunits and ATP- ...
... alpha), β (beta), and γ (gamma). In the inactive state, the three subunits associate together and the α-subunit binds GDP. G ... They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ... GTP-binding to the α-subunit causes dissociation of the β- and γ-subunits. Furthermore, the three subunits, α, β, and γ have ...
... core binding factor alpha 2 subunit MeSH D12.776.930.155.200.300 - core binding factor alpha 3 subunit MeSH D12.776.930.316. ... nf-e2 transcription factor, p45 subunit MeSH D12.776.930.155.200.100 - core binding factor alpha 1 subunit MeSH D12.776.930.155 ... CCAAT-binding factor MeSH D12.776.930.127.124.500 - CCAAT-enhancer-binding protein-alpha MeSH D12.776.930.127.124.750 - CCAAT- ... alpha subunit MeSH D12.776.930.125.750.590 - myod protein MeSH D12.776.930.125.750.595 - myogenic regulatory factor 5 MeSH ...
A highly conserved core, amino acid repeat regions, and homologies with translation elongation factors". J Biol Chem. 266 (3): ... 2002). "Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex". Biochem. Biophys ... 2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97 ... identification of RNA-binding residues and functional implications for tandem repeats". Biochemistry. 39 (51): 15775-82. doi: ...
A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-YGYGGT-3' found in a ... Core binding factor RUNX1 RUNX2 GRCh38: Ensembl release 89: ENSG00000020633 - Ensembl, May 2017 GRCm38: Ensembl release 89: ... "Isolation of PEBP2 alpha B cDNA representing the mouse homolog of human acute myeloid leukemia gene, AML1". Oncogene. 8 (3): ... Runt-related transcription factor 3 is a protein that in humans is encoded by the RUNX3 gene. This gene encodes a member of the ...
This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that ... pre-initiation complex on a eukaryotic core promoter involve the effects of TFIIA on the interaction between TATA-binding ... TFIIA-alpha and beta-like factor is a protein that in humans is encoded by the GTF2A1L gene. The assembly and stability of the ... "Entrez Gene: ALF TFIIA-alpha/beta-like factor". Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap ...
... general transcription factor 2H subunit 5) is also known as the TTD group A (TTDA) subunit (and as Tfb5). The TTDA subunit is ... and part of a six-subunit complex of Rad3, Tfb1, Tfb2, Tfb4, Tfb5, and Ssl1 (referred to as core) In humans, the function of ... TTDA is present both bound to TFIIH, and as a free fraction that shuffles between the cytoplasm and nucleus; induction of NER- ... These proteins have a structural motif consisting of a 2-layer sandwich structure with an alpha/beta plait topology. ...
... caused by the alpha decay of actinium-227.[35] Perey then attempted to determine the proportion of beta decay to alpha decay in ... Gottschlich, Michele M. (2001). The Science and Practice of Nutrition Support: A Case-based Core Curriculum. Kendall Hunt. p. ... The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... Solid phenyllithium forms monoclinic crystals can be described as consisting of dimeric Li2(C6H5)2 subunits. The lithium atoms ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
protein kinase binding. • core promoter binding. • RNA polymerase II transcription coactivator activity. • transcription factor ... ribosomal large subunit binding. • protein homodimerization activity. • ribosomal small subunit binding. • unfolded protein ... regulation of eIF2 alpha phosphorylation by dsRNA. • positive regulation of cell cycle G2/M phase transition. • negative ... activating transcription factor binding. • rRNA binding. • protein N-terminus binding. • chromatin binding. ...
BDNF is made in the endoplasmic reticulum and secreted from dense-core vesicles. It binds carboxypeptidase E (CPE), and the ... regulates the expression and synaptic delivery of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid receptor subunits ... receptor binding. • neurotrophin TRKB receptor binding. • growth factor activity. • GO:0001948 protein binding. ... BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor. ...
... a 9-subunit base that binds directly to the α ring of the 20S core particle, and a 10-subunit lid. Six of the nine base ... Degradation of Aux/IAA proteins derepresses transcription factors in the auxin-response factor (ARF) family and induces ARF- ... which serve as docking domains for the regulatory particles and the alpha subunits N-termini (Pfam PF10584) form a gate that ... The mechanisms by which it binds to the core particle through the C-terminal tails of its subunits and induces α-ring ...
The alpha helical anticodon binding domain of Arginyl, Glycyl and Cysteinyl-tRNA synthetases is known as the DALR domain after ... Alignment of the core domains of aminoacyl-tRNA synthetases class I and class II. Essential binding site residues (Backbone ... It aminoacylates at the 3'-OH of a terminal adenosine on tRNA, and is usually dimeric or tetrameric (two or four subunits, ... and an anticodon binding domain (which interacts mostly with the anticodon region of the tRNA and ensures binding of the ...
... s can bind to other proteins as well as to small-molecule substrates. When proteins bind specifically to other copies of ... Kauzmann W (May 1956). "Structural factors in protein denaturation". Journal of Cellular Physiology. 47 (Suppl 1): 113-31. doi: ... Quaternary structure: the structure formed by several protein molecules (polypeptide chains), usually called protein subunits ... The membrane alone has a hydrophobic core through which polar or charged molecules cannot diffuse. Membrane proteins contain ...
... the alpha helix). Linus Pauling was the first to identify[29] the 3.6 amino acids per helix turn ratio of the alpha helix. ... It, however, requires a five base binding between the mRNA and tRNA with a flip of the anti-codon creating a triplet coding, ... Such a code might be "degenerate", with 4×4×4=64 possible triplets of the four nucleotide subunits while there were only 20 ... In 1960, Crick accepted an honorary fellowship at Churchill College, Cambridge, one factor being that the new college did not ...
... the position where the substrate binds, may influence factors like binding affinity of ligands, stabilization of substrates ... The number of sub-units making up cellulosomes can also determine the rate of enzyme activity.[12] ... McCleary BV (November 1980). "New chromogenic substrates for the assay of alpha-amylase and (1 leads to 4)-beta-D-glucanase". ... construction of the catalytic core, expression, and X-ray structure at 1.75 Å resolution". Biochemistry. 36 (51): 16032-9. doi: ...
Each tropomyosin molecule has a smaller calcium-binding protein called troponin bound to it. All thin filaments are attached to ... The filaments are organized into repeated subunits along the length of the myofibril. These subunits are called sarcomeres. ... They run through the core of each thick filament and anchor it to the Z-line, the end point of a sarcomere. Titin also ... Troponin and the associated tropomyosin undergo a conformational change after calcium binding and expose the myosin binding ...
Each AMPAR has four sites to which an agonist (such as glutamate) can bind, one for each subunit.[5] The binding site is ... Armstrong N, Sun Y, Chen GQ, Gouaux E (October 1998). "Structure of a glutamate-receptor ligand-binding core in complex with ... "Phosphorylation of the alpha-amino-3-hydroxy-5-methylisoxazole4-propionic acid receptor GluR1 subunit by calcium/calmodulin- ... "Postsynaptic factors in the expression of long-term potentiation (LTP): increased glutamate receptor binding following LTP ...
... sandwiched between the Walker A and B motifs of one subunit and the LSGGQ motif of the other subunit. The maltose binding ... heme-binding protein, and alkaline protease), heme, hydrolytic enzymes, S-layer proteins, competence factors, toxins, ... In addition, a gap in the protein is accessible directly from the hydrophobic core of the inner leaflet of the membrane bilayer ... The T domains are each built of typically 10 membrane spanning alpha helices, through which the transported substance can cross ...
This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding ... C1QBP has been shown to interact with Protein kinase D1,[10] BAT2,[11] PRKCD,[10] PKC alpha[10] and Protein kinase Mζ.[10] ... "Interaction between complement receptor gC1qR and hepatitis C virus core protein inhibits T-lymphocyte proliferation". J Clin ... transcription factor binding. • protein binding. • hyaluronic acid binding. • kininogen binding. • mitochondrial ribosome ...
Each subunit consists of three domains. At the carboxyl terminal of the peptide chain there's a short alpha helix domain that ... oxygen binding. • metal ion binding. • dopamine binding. • enzyme binding. • oxidoreductase activity. • iron ion binding. • ... response to growth factor. • response to ethanol. • cellular response to glucose stimulus. • phthalate metabolic process. • ... allows tetramerization.[15] The central ~300 amino acids make up a catalytic core, in which all the residues necessary for ...
... of the gene product binds the θ subunit and carries out the exonuclease function and the C-terminus binds the α subunit ... "The proofreading exonuclease subunit epsilon of Escherichia coli DNA polymerase III is tethered to the polymerase subunit alpha ... The ε subunit is one of three core proteins in the DNA polymerase complex. It functions as a 3'→5' DNA directed proofreading ... The ε subunit is stabilized by the θ subunit within the complete polymerase complex.[7] ...
transcription factor activity, sequence-specific DNA binding. • transcription factor binding. • RNA polymerase II core promoter ... 2009). "The correlation of hepatocyte nuclear factor 4 alpha and 3 beta with hepatitis B virus replication in the liver of ... "Foxa2 and MafA regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression". J. Mol. ... transcription regulatory region DNA binding. • RNA polymerase II transcription factor activity, sequence-specific DNA binding. ...
14 alpha particles) is easily produced from lighter nuclei in the alpha process in nuclear reactions in supernovae (see silicon ... The inner core of the Earth is generally presumed to consist of an iron-nickel alloy with ε (or β) structure.[10] ... Hemoglobin bound to carbon monoxide is known as carboxyhemoglobin. This effect also plays a minor role in the toxicity of ... The ensuing availability of inexpensive iron was one of the factors leading to the Industrial Revolution. Toward the end of the ...
InaD contains five binding domains called PDZ domain proteins, which specifically bind the C termini of target proteins. ... Drosophila differentially expresses a core group of 252 genes upon infection with most bacteria. This core group of genes is ... Bakker K (1961). "An analysis of factors which determine success in competition for food among larvae of Drosophila ... A 2016 study concluded that food supplementation with 10-mM alpha-ketoglutarate decreased Drosophila alcohol sensitivity over ...
The activated Gs alpha subunit binds to and activates an enzyme called adenylyl cyclase, which, in turn, catalyzes the ... crystallised the PKA Cα subunit, which revealed the bi-lobe structure of the protein kinase core for the very first time, ... Long term memory is dependent on the CREB transcription factor, regulated by PKA. A study done on drosophila reported that an ... Two cAMP molecules bind to each PKA regulatory subunit. *The regulatory subunits move out of the active sites of the catalytic ...
In the presence of the R subunit, the complex can also act as an endonuclease, binding to the same target sequence but cutting ... A conserved enzyme core with variable target-recognizing domains". J. Mol. Biol. 206 (2): 305-12. doi:10.1016/0022-2836(89) ... hsdM contains an alpha-helical domain at the N-terminus, the HsdM N-terminal domain.[7] ... The type I restriction and modification system is composed of three polypeptides R, M and S. The M (hsdM) and S subunits ...
For example, a protein called eukaryotic initiation factor-2 (eIF-2) can bind to the smaller subunit of the ribosome, starting ... The two reacting molecules are the alpha amino group of one amino acid and the alpha carboxyl group of the other amino acids. A ... In a hydrophilic environment such as cytosol, the hydrophobic amino acids will concentrate at the core of the protein, while ... These subunits surround the mRNA strand. The larger subunit contains three binding sites: A (aminoacyl), P (peptidyl), and E ( ...
DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... "New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ...
ubiquitin protein ligase binding. • importin-alpha family protein binding. • disordered domain specific binding. • core ... DNA binding. • core promoter binding. • transcription factor activity, sequence-specific DNA binding. • transcription ... Within each subunit, there are a variety of protein binding sites, as well as a total of 15 possible phosphorylation sites. ... transcription factor binding. • RNA polymerase II activating transcription factor binding. • phosphoprotein binding. • kinase ...
An important factor in the binding of the first generation NNRTIs, such as nevirapine, is the butterfly-like shape. Despite ... The cloroindole part interacted with the hydrophobic core of the pocket and influenced the binding mode of the R120393 so it ... Å from the catalytic site in the palm domain of the p66 subunit site of the enzyme. The NNRTI binding pocket (NNIBP) contains ... From alpha-anilinophenylacetamide (alpha-APA) to imidoyl thiourea (ITU)". Bioorganic & Medicinal Chemistry Letters. 11 (17): ...
Most vegetable oils are rich in linoleic acid (safflower, sunflower, and corn oils). Alpha-linolenic acid is found in the green ... Malinauskas T (2008). "Docking of fatty acids into the WIF domain of the human Wnt inhibitory factor-1". Lipids. 43 (3): 227-30 ... The steroids, all derived from the same fused four-ring core structure, have different biological roles as hormones and ... Polyketides are synthesized by polymerization of acetyl and propionyl subunits by classic enzymes as well as iterative and ...
The same protein may act as the subunit of both the pentamers and hexamers or they may be composed of different proteins.[81]. ... When the adaptive immune system of a vertebrate encounters a virus, it produces specific antibodies that bind to the virus and ... Counting these areas and multiplying by the dilution factor allowed him to calculate the number of viruses in the original ... Safety, tolerability and efficacy of peginterferon alpha-2a and ribavirin in chronic hepatitis C in clinical practice: The ...
0001204 DNA-binding transcription factor activity. • 5'-deoxyribose-5-phosphate lyase activity. • transcription factor binding ... "Functional interaction between the DNA binding subunit trimerization domain of NF-Y and the high mobility group protein HMG-I(Y ... core motif, a novel cross-linking domain located between the second and third AT-hook, and a C-terminal acidic tail ... "Regulation of cell-type-specific interleukin-2 receptor alpha-chain gene expression: potential role of physical interactions ...
Core Subunitsa 1. NDUFS7 / PSST / NUKM. NDUS7_HUMAN. NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial EC ... Complex I contains a ubiquinone binding pocket at the interface of the 49-kDa and PSST subunits. Close to iron-sulfur cluster ... Assembly factor proteins[31] 47. NDUFAF1c. CIA30_HUMAN. NADH dehydrogenase [ubiquinone] 1 alpha subcomplex, assembly factor 1. ... alpha/beta subcomplex, 1, 8kDa. *NDUFAF1 - NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, assembly factor 1 ...
Zipper Coiled-Coil Domains of the cGMP-Dependent Protein Kinase I alpha and its Interaction with the Myosin Binding Subunit of ... Leucine zipper coiled-coil helices & DNA-binding helices: transcription factor Max (PDB file 1HLO) ... in the hydrophobic core, and one containing predominantly polar amino acids oriented toward the solvent-exposed surface of the ... DNA bindingEdit. α-Helices have particular significance in DNA binding motifs, including helix-turn-helix motifs, leucine ...
Diagram of the 12 subunit catalytic core of the cubic 24 subunit human E2k. [18] (C) Ribbon diagram of the 8 Monomer E3 subunit ... File:Structure of alpha-KGDHC.jpg Figure 7: Structure of the alpha-KGDHC subunits (A) Ribbon diagram of the homo-dimer ... The first factor was a decrease in transcription (mRNA levels) of the subunits comprising α-KGDHC. As shown in Figure 13, after ... It is an 8 monomer that can be divided into 4 domains; the FAD-binding domain (residues 1-149), the NAD+-binding domain ( ...
What is core-binding factor, runt domain, alpha subunit 1? Meaning of core-binding factor, runt domain, alpha subunit 1 medical ... What does core-binding factor, runt domain, alpha subunit 1 mean? ... core-binding factor, runt domain, alpha subunit 1 explanation ... Looking for online definition of core-binding factor, runt domain, alpha subunit 1 in the Medical Dictionary? ... redirected from core-binding factor, runt domain, alpha subunit 1) RUNX2. A gene on chromosome 6p21 that encodes a nuclear ...
SL3/AKV core-binding factor alpha A subunit. Additional Information & Resources. Tests Listed in the Genetic Testing Registry. ... The RUNX2 protein is a transcription factor, which means it attaches (binds) to specific regions of DNA and helps control the ... 2013 Jan;24(1):130-3. doi: 10.1097/SCS.0b013e3182636b7e. Review. Citation on PubMed ... Page last updated on 18 August 2020 Page last reviewed: 1 August 2017 ...
Core Binding Factor Alpha 2 Subunit * Core Binding Factor Alpha 3 Subunit ... Core Binding Factor Alpha 2 Subunit * Core Binding Factor Alpha 3 Subunit ... Gary S Stein 1 , Jane B Lian, Andre J van Wijnen, Janet L Stein, Martin Montecino, Amjad Javed, Sayyed K Zaidi, Daniel W Young ... organization and combinatorial assembly of nucleic acids and regulatory factors within the three-dimensional context of nuclear ...
Core Binding Factor Alpha 1 Subunit * Silymarin * Sp7 Transcription Factor * Sp7 protein, human ... Xiaozhou Ying 1 , Liaojun Sun, Xiaowei Chen, Huazi Xu, Xiaoshan Guo, Hua Chen, Jianjun Hong, Shaowen Cheng, Lei Peng ... and Runt-related transcription factor 2 (RUNX2). The BMP antagonist noggin and its receptor kinase inhibitors dorsomorphin and ... 1 Department of Orthopedic Surgery, The Second Affiliated Hospital of Wenzhou Medical College, 109 Xue Yuan xi Road, Wenzhou ...
... core-binding factor alpha subunit 1 (Cbfa1; 7.2-fold), osteocalcin (OC; 430-fold), and BSP (BSP; 5.7-fold) transcripts (Fig. 1 ... B and C) Newly formed vessels, identified by CD31+ endothelial cells, penetrated the outer matrix and reached the inner core in ... an inner hypertrophic core (B, D, and F) rich in GAG, Col II, and Col X, and an outer mineralized rim (B, H, J, and L) with a ... 1997) Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. ...
0 (BMP2 protein, human); 0 (Bone Morphogenetic Protein 2); 0 (Core Binding Factor Alpha 1 Subunit); 0 (Drugs, Chinese Herbal); ... 0 (Enzyme Inhibitors); EC 3.2.1.1 (alpha-Amylases); EC 3.4.21.62 (Subtilisin). ... Subunidade alfa 1 de Fator de Liga o ao Core/gen tica. Seres Humanos. Sistema de Sinaliza o das MAP Quinases/efeitos dos f ... Two pairs of diastereoisomers, namely (3Z)-(3S,8S,3aS,6R)-4,5-dehydro-3.3a,8.6-diligustilide (1) and (3Z)-(3S,8R,3aS,6R ...
The analysis leads to a better insight of proteins that bind to DNA, regulate DNA, and function in chromatin remodeling. The ... The study summarizes transcriptional regulation factors interacting and cooperating at promoter regions that regulate gene ... Core-binding factor alpha-subunit 1. Osteoblast-specific transcription factor. Cleidocranial dysplasia murine Cbfa1+/- ... Transcription factors bind to regulatory elements upstream of transcription start sites and interact with other factors to ...
Core Binding Factor Alpha 1 Subunit. Enzyme Induction. Okadaic Acid. Bone Remodeling. Mesenchymal Stem Cells. Osteoblasts. ... Transforming Growth Factor beta1. Down-Regulation. Up-Regulation. 3 Untranslated Regions. Activating Transcription Factor 3. ... Core Binding Factor Alpha 1 Subunit. 3. 2015. 2018. March 2017. Enzyme Induction. 1. 2017. 2017. May 2017. ...
Like other transcription factors, the RUNX1 protein attaches (binds) to specific regions of DNA and helps control the activity ... The RUNX1 gene provides instructions for making a protein called runt-related transcription factor 1 (RUNX1). ... SL3/AKV core-binding factor alpha B subunit. Additional Information & Resources. Tests Listed in the Genetic Testing Registry. ... Core binding factor acute myeloid leukemia. A rearrangement (translocation) of genetic material involving the RUNX1 gene is ...
... core binding factor plays a key role in several development pathways and in human disease; has been sequenced ... Transcription Factors: 20597*Core Binding Factors: 223*Core Binding Factor alpha Subunits: 18*core binding factor alpha: 16 ... core binding factor alpha. Subscribe to New Research on core binding factor alpha ... 11/07/2000 - "Binding of CBFalpha/AML/PEBP2alpha (core binding factor alpha/acute myelogenous leukemia/polyoma enhancer binding ...
Core-binding factor subunit alpha-1; Short=CBF-alpha-1;AltName: Full=Oncogene AML-3;AltName: Full=Osteoblast-specific ... the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. Virology. 1993 May;194(1):314-31 ... J:19082 Ogawa E, et al., PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila ... J:49076 Xiao ZS, et al., Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor. ...
SL3/AKV core binding factor alpha A subunit. *SL3/AKV core-binding factor alpha A subunit ... CBF binds to the core site, 5-PYGPYGGT-3, of a number of enhancers and promoters, including murine leukemia virus, ... Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of ... alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. ...
Core-binding factor subunit alpha-1. FDP:. Fixed dental prostheses. RUNX2:. Runt-related transcription factor 2 ... a transcription factor on chromosome 6p21, have been identified as the cause [3]. This transcription factor is responsible for ... factors such as risk of chipping, esthetics [34], and costs must be weighed. In this case, no ceramic-veneered FDPs were ... 1). Teeth 1, 5, 16, 19, 20, and 21 were missing. Teeth 6, 8B, 9A, 9B, 11, 15, 18, 19, 22-28, 31, and 32 were retained and ...
CBF-alpha-2; core binding factor alpha 2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit ... PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; runt domain, alpha subunit 2; Runt- ... SL3/AKV core-binding factor alpha B subunit; transcription factor Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF-alpha-2; ... DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding transcription factor binding ...
... to runt-related transcription factor 2 (RUNX2) in the mDE6 cells. Several s ... Core Binding Factor Alpha 1 Subunit/genetics*/metabolism. *Epithelial Cells/metabolism*. *Proto-Oncogene Proteins c-akt/ ... and can induce the expression of runt-related transcription factor 2 (RUNX2) during the development of the tooth germ.The ... and can induce the expression of runt-related transcription factor 2 (RUNX2) during the development of the tooth germ. RUNX2 ...
SL3/AKV core binding factor alpha B subunit. *SL3/AKV core-binding factor alpha B subunit ... Core binding factor alpha 2 subunit. *Core binding factor runt domain alpha subunit 2 ... The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. Isoform AML-1L interferes ... CBF binds to the core site, 5-PYGPYGGT-3, of a number of enhancers and promoters, including murine leukemia virus, ...
... core-binding factor subunit alpha-1 (Cbfa1) and receptor activator of nuclear factor kappa-B ligand (RANKL), which is regulated ... Aliskiren is a drug of the second generation of renin inhibitors, and it binds to the binding site of renin essential for its ... Renin inhibitors bind to the active site of renin and inhibit its binding to angiotensinogen, which is the rate-determining ... Aliskiren binds to the binding site of renin essential for its activity thereby reducing plasma renin activity and suppressing ...
Core Binding Factor alpha Subunits * Transcriptional Activation * leucine-rich repeat proteins * Leukemia ...
Core Binding Factor Alpha 1 Subunit. *Gene Expression Regulation, Developmental*. *In Situ Hybridization ... One crucial regulatory gene in the pathway encodes the transcription factor Runx2, which in mouse is required for ...
Core Binding Factor Alpha 1 Subunit. Female. Femoral Neck Fractures. Femur Neck. Genetic Predisposition to Disease. Glutamine. ... transcription factor RUNX2. vitamin D receptor. adult. aged. allele. article. bone densitometry. bone density. female. femur ... RUNX2 is an essential transcription factor required for skeletal development and cartilage formation. Haploinsufficiency of ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Calculated molecular weight: ... Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP(CBFA2T3 antibody) at dilution of 1:1000 ... Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP(CBFA2T3 antibody) at dilution of 1:1000 ... Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP(CBFA2T3 antibody) at dilution of 1:1000 ...
Core Binding Factor Alpha 1 Subunit/genetics. *DNA Primers. *Flow Cytometry. *Gene Expression Profiling ... Role of Slug transcription factor in human mesenchymal stem cells. Torreggiani E, Lisignoli G, Manferdini C, Lambertini E, ... Role of Slug transcription factor in human mesenchymal stem cells. Torreggiani E, Lisignoli G, Manferdini C, Lambertini E, ...
Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that ... The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants ... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for ... The phosphorylation state of Runx2 also mediates its DNA-binding activity. The Runx2 DNA-binding activity is correlated with ...
... core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5-pygpyggt-3, of a number of enhancers and ... The protein is a heterodimer of alpha- and beta-subunits. The alpha-subunit binds DNA as a monomer, and appears to have a role ... highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit ... The region of shared similarity, known as the Runt domain, is responsible for DNA-binding and protein-protein interaction. In ...
... bone differentiation depends on pRb coactivation of the RUNT-RELATED TRANSCRIPTION FACTOR2/CORE-BINDING FACTOR SUBUNIT ALPHA-1 ... pRB is known to promote cell cycle exit by binding to members of the E2F family of transcription factors, thus interfering with ... pRB acts as a transcriptional regulator primarily via a mechanism that consists of binding to the main transcription factor ... a transcription factor involved in cell differentiation. We show that both RBR and the cytokinin-dependent transcription factor ...
Core Binding Factor Alpha 1 Subunit Genes Co-Repressor Proteins Gene expression ... an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. Runx2, ... an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. Runx2, ... an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. Runx2, ...
The transforming growth factor beta (TGF-beta) family member bone morphogenetic protein-3B (BMP-3B/GDF10) is regarded as a ... The ectopic expression of Runx2, but not DNA binding mutant Runx2, in normal lung fibroblast cells and lung cancer cells ... The Runt-related transcription factor Runx2 is essential for bone development but is also implicated in progression of several ... Core Binding Factor Alpha 1 Subunit; Bone Morphogenetic Protein 3; Lung Neoplasms ...
Core Binding Factor Alpha 1 Subunit / metabolism. Eyelid Neoplasms / metabolism. Osteoblasts / metabolism ... Chemical-registry-number] 0 / Antigens, Differentiation; 0 / Core Binding Factor Alpha 1 Subunit ... In this review, we discuss the risk factors, pathology, molecular biology, clinical features, and management of eyelid basal ... 1. Jankovic I, Kovacevic P, Visnjic M, Jankovic D, Binic I, Jankovic A: Does incomplete excision of basal cell carcinoma of the ...
Moreover, we show that some of these sites are direct target genes of these transcription factors as shown by ChIP assays. ... We next scanned the upstream promoter regions of the 394 genes for the presence of evolutionarily conserved binding sites for ... transcription factors implicated in growth plate maturation such as estrogen receptor (ER), androgen receptor, ELK1, STAT5B, ... Adolescent Development; Core Binding Factor Alpha 1 Subunit; Gene Expression Regulation, Developmental; Growth Plate; Hormones ...
Mouse runt-related transcription factor 2 ELISA Kit-NP_001139510.1 (MBS934869) product datasheet at MyBioSource, ELISA Kits ... Acute myeloid leukemia 3 protein; Core-binding factor subunit alpha-1; CBF-alpha-1; Oncogene AML-3; Osteoblast-specific ... PEA2-alpha A; PEBP2 alpha A; AKV core binding factor; core binding factor alpha 1; runt domain, alpha subunit 1; acute myeloid ... DNA binding; histone deacetylase binding; bHLH transcription factor binding; chromatin binding; transcription factor binding; ...
  • RUNX2 is essential for osteoblastic differentiation and skeletal morphogenesis, and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. (thefreedictionary.com)
  • The RUNX2 protein is a transcription factor, which means it attaches (binds) to specific regions of DNA and helps control the activity of particular genes. (medlineplus.gov)
  • Silibinin also increased the mRNA expressions of Collagen type I (COL-I), ALP, Osteocalcin (OCN), Osterix, bone morphogenetic protein-2 (BMP-2) and Runt-related transcription factor 2 (RUNX2). (nih.gov)
  • Parathyroid Hormone-responsive Smad3-related Factor, Tmem119, Promotes Osteoblast Differentiation and Interacts with the Bone Morphogenetic Protein-Runx2 Pathway. (jax.org)
  • Foxo1 mediates insulin-like growth factor 1 (IGF1)/insulin regulation of osteocalcin expression by antagonizing Runx2 in osteoblasts. (jax.org)
  • In previous studies by our group, we reported that thymosin beta 4 (Tb4) is closely associated with the initiation and development of the tooth germ, and can induce the expression of runt-related transcription factor 2 (RUNX2) during the development of the tooth germ. (nih.gov)
  • Schematic illustration of the putative signaling pathways from thymosin beta 4 (Tb4) to runt-related transcription factor 2 (RUNX2) in the mDE6 cells. (nih.gov)
  • RUNX2 can upregulate the expression of downstream biological effectors, including odontogenesis-related genes and calcification-related factors. (nih.gov)
  • One crucial regulatory gene in the pathway encodes the transcription factor Runx2, which in mouse is required for differentiation of all osteoblasts and the proper development of a subset of hypertrophic chondrocytes. (zfin.org)
  • Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that in humans is encoded by the RUNX2 gene. (wikipedia.org)
  • RUNX2 is a key transcription factor associated with osteoblast differentiation. (wikipedia.org)
  • The phosphorylation state of Runx2 also mediates its DNA-binding activity. (wikipedia.org)
  • The Runx2 DNA-binding activity is correlated with cellular proliferation, which suggests Runx2 phosphorylation may also be related to Runx2-mediated cellular proliferation and cell cycle control. (wikipedia.org)
  • Here we show that the endogenous Yes-associated protein (YAP), a mediator of Src/Yes signaling, interacts with the native Runx2 protein, an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. (elsevier.com)
  • BACKGROUND: The Runt-related transcription factor Runx2 is essential for bone development but is also implicated in progression of several cancers of breast, prostate and bone, where it activates cancer-related genes and promotes invasive properties. (umassmed.edu)
  • The ectopic expression of Runx2, but not DNA binding mutant Runx2, in normal lung fibroblast cells and lung cancer cells resulted in suppression of BMP-3B levels. (umassmed.edu)
  • MBS934869 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the runt-related transcription factor 2 (RUNX2) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • Standards and samples are pipetted into the wells and any RUNX2 present is bound by the immobilized antibody. (mybiosource.com)
  • Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RUNX2 bound in the initial step. (mybiosource.com)
  • Title: Regulation of tissue inhibitor of metalloproteinase 1 gene transcription by RUNX1 and RUNX2. (genscript.com)
  • Stromal cells of fibrodysplasia ossificans progressiva lesions express smooth muscle lineage markers and the osteogenic transcription factor Runx2/Cbfa-1: clues to a vascular origin of heterotopic ossification? (nih.gov)
  • These cells co-express multiple SM lineage markers along with multiple proteins associated with bone formation including the obligate osteogenic transcription factor Runx2/Cbfa-1. (nih.gov)
  • The aim of this study was to determine if DNA polymorphism within runt-related gene 2 (RUNX2)/core binding factor A1 (CBFA1) is related to bone mineral density (BMD). (cdc.gov)
  • RUNX2 is a transcription factor essential for osteoblast differentiation and skeletal morphogenesis. (cdc.gov)
  • The Runx2 gene is a master transcription factor of bone and plays a role in all stages of bone formation. (nih.gov)
  • Immunofluorescence, immunohistochemistry, and quantitative polymerase chain reaction were performed to evaluate runt-related transcription factor 2 (RUNX2), osteonectin, interleukin (IL)-13, and RUNX2 downstream gene expression. (bvsalud.org)
  • Osteoblast differentiation is controlled by main bone specific transcription factors including Osterix, distal-less homeobox 5 (Dlx5), and Runt-related transcription factor 2 (Runx2). (elsevier.com)
  • The deletion or mutation of the Dlx5 and Runx2 binding sites significantly suppressed Osterix promoter activity in shPP2A cells. (elsevier.com)
  • These results indicate that Dlx5 and Runx2 are critical factors for the upregulated Osterix expression in shPP2A cells, which is considered to be important for the accelerated osteoblast differentiation in these cells. (elsevier.com)
  • Core-binding factor beta interacts with Runx2 and is required for skeletal development. (labome.org)
  • MiR-223 and miR-19a were found to regulate the expression of TWIST and Runx2, influence the RANKL-RANK pathway and the expression of MCP-1, and finally regulate the pathophysiological process of osteolytic bone destruction. (antibodies-online.com)
  • Core binding factors are heterodimeric transcription factors containing a DNA binding Runx1, Runx2, or Runx3 subunit, along with a non DNA binding CBF beta subunit. (ox.ac.uk)
  • Identification of a promoter element within the zebrafish colXalpha1 gene responsive to runx2 isoforms Osf2/Cbfa1 and til-1 but not to pebp2alphaA2. (ualg.pt)
  • We report here the functional analysis of the zebrafish (Danio rerio) collagen Xalpha1 gene (colXalpha1) promoter with the identification of a region responsive to two isoforms of the runt domain transcription factor runx2. (ualg.pt)
  • METHODS AND RESULTS: Immunohistochemical methods were used to detect the expression of osteogenic indicators Runx2, OPN and inflammation indicators IL-1ß and p-NF-κB in valve specimens of CAVD patients(N = 3) and normal controls(N = 1). (bvsalud.org)
  • In addition, it has already been demonstrated that SIRT1 regulates the activity of the osteogenic transcription factor runt-related transcription factor 2 (Runx2). (bvsalud.org)
  • Role of Slug transcription factor in human mesenchymal stem cells. (nih.gov)
  • We review recent findings that are consistent with an active role for Runx proteins as scaffolds for integration, organization and combinatorial assembly of nucleic acids and regulatory factors within the three-dimensional context of nuclear architecture. (nih.gov)
  • The analysis leads to a better insight of proteins that bind to DNA, regulate DNA, and function in chromatin remodeling. (biomedcentral.com)
  • Genes in eukaryotic cells are regulated in "active regions," in which chromatin structure is "open" and accessible to DNA-binding proteins and "silent regions" where "packed" chromatin renders the DNA inaccessible. (biomedcentral.com)
  • An important motif presented in the promoter is an A/T-rich region that binds proteins with an A/T-hook motif. (biomedcentral.com)
  • Together, these proteins form one version of a complex known as core binding factor (CBF). (medlineplus.gov)
  • RUNX1 belongs to the mammalian RUNX family of DNA binding proteins that regulate the expression of genes involved in cellular differentiation and cell cycle progression. (thermofisher.com)
  • Gag-Pol polyprotein: Mediates, with Gag polyprotein, the essential events in virion assembly, including binding the plasma membrane, making the protein-protein interactions necessary to create spherical particles, recruiting the viral Env proteins, and packaging the genomic RNA via direct interactions with the RNA packaging sequence (Psi). (uniprot.org)
  • RUNX proteins are evolutionarily conserved transcription factors known to be involved in various developmental processes. (elsevier.com)
  • We also discuss the later stages of hematopoiesis for which members of the core binding factor family are required, as well as the recently described roles for these proteins in autoimmunity. (ox.ac.uk)
  • It performs this function by attaching (binding) to proteins that normally turn genes on and blocking their activity. (nih.gov)
  • Guo C, Hu Q, Yan C, Zhang J. Multivalent binding of the ETO corepressor to E proteins facilitates dual repression controls targeting chromatin and the basal transcription machinery. (nih.gov)
  • RUNX proteins form a heterodimeric complex with CBFβ which confers increased DNA binding and stability to the complex. (hitchhikersgui.de)
  • The transcription of RUNX1 is regulated by 2 enhancers (regulatory element 1 and regulatory element 2), and these tissue specific enhancers enable the binding of lymphoid or erythroid regulatory proteins, therefore the gene activity of RUNX1 is highly active in the haematopoietic system. (hitchhikersgui.de)
  • E proteins activate transcription by binding to regulatory E-box sequences on target genes as heterodimers or homodimers, and are inhibited by heterodimerization with inhibitor of DNA-binding (class IV) helix-loop-helix proteins. (cancerindex.org)
  • STAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. (merckmillipore.com)
  • The proteins that guide RNA polymerase to genes are the sigma factors. (wikibooks.org)
  • In genetics , a promoter is a sequence of DNA to which proteins bind that initiate transcription of a single RNA from the DNA downstream of it. (wikipedia.org)
  • Promoters contain specific DNA sequences such as response elements that provide a secure initial binding site for RNA polymerase and for proteins called transcription factors that recruit RNA polymerase. (wikipedia.org)
  • A promoter is induced in response to changes in abundance or conformation of regulatory proteins in a cell, which enable activating transcription factors to recruit RNA polymerase. (wikipedia.org)
  • Proteins belonging to the MADS family function as dimers, the primary DNA-binding element of which is an anti-parallel coiled coil of two amphipathic alpha-helices, one from each subunit. (ebi.ac.uk)
  • A common chromosomal translocation in acute myeloid leukemia (AML) involves the AML1 (acute myeloid leukemia 1, also called RUNX1, core binding factor protein (CBF alpha), and PEBP2 alpha B) gene on chromosome 21 and the ETO (eight-twenty one, also called MTG8) gene on chromosome 8. (curehunter.com)
  • One of the genes that encodes a CBF alpha subunit is AML1, also called Cbf alpha 2. (curehunter.com)
  • PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila runt gene and the human AML1 gene. (jax.org)
  • The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. (genecards.org)
  • Runt-related transcription factor 1 ( RUNX1 ) also known as acute myeloid leukemia 1 protein (AML1) or core-binding factor subunit alpha-2 (CBFA2) is a protein that in humans is encoded by the RUNX1 gene . (hitchhikersgui.de)
  • The RUNX1 gene provides instructions for making a protein called runt-related transcription factor 1 (RUNX1). (medlineplus.gov)
  • Like other transcription factors, the RUNX1 protein attaches (binds) to specific regions of DNA and helps control the activity of particular genes. (medlineplus.gov)
  • This protein interacts with another protein called core binding factor beta or CBFβ (produced from the CBFB gene), which helps RUNX1 bind to DNA and prevents it from being broken down. (medlineplus.gov)
  • Transcription factor RUNX1 promotes survival of acute myeloid leukemia cells. (medlineplus.gov)
  • RUNX1 belongs to the runt domain family of transcription factors and regulates target gene expression through forming a heterodimeric DNA-binding complex with CBFB. (acris-antibodies.com)
  • Proximity Ligation Analysis (PLA) of protein-protein interactions between CDK6 and RUNX1 HeLa cells were stained with anti-CDK6 rabbit purified polyclonal 1:1200 and anti-RUNX1 mouse monoclonal antibody 1:50. (acris-antibodies.com)
  • WB Suggested Anti-RUNX1 Antibody Titration: 0.2-1 ug/ml. (acris-antibodies.com)
  • Transcription factor Runx1 inhibits proliferation and promotes developmental maturation in a selected population of inner olfactory nerve layer olfactory ensheathing cells. (genscript.com)
  • Zebrafish embryos lacking Rad21 (show RAD21 ELISA Kits ), or cohesin subunit Smc3 (show SMC3 ELISA Kits ), fail to express runx3 and lose hematopoietic runx1 (show RUNX1 ELISA Kits ) expression in early embryonic development. (antibodies-online.com)
  • The protein produced from the normal RUNX1 gene is part of a protein complex known as core binding factor (CBF). (nih.gov)
  • RUNX1 is a transcription factor that regulates the differentiation of hematopoietic stem cells into mature blood cells. (hitchhikersgui.de)
  • These domains are necessary for RUNX1 to mediate DNA binding and protein-protein interactions respectively. (hitchhikersgui.de)
  • The pro-haematopoietic effects of EETs were conserved in the developing zebrafish embryo, where 11,12-EET promoted HSPC specification by activating a unique activator protein 1 (AP-1) and runx1 transcription program autonomous to the haemogenic endothelium. (zfin.org)
  • The protein (known as acute myeloid leukemia 1 protein, oncogene AML-1, core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5'-pygpyggt-3', of a number of enhancers and promoters. (wikipedia.org)
  • SRF function is essential for transcriptional regulation of numerous growth-factor-inducible genes, such as c-fos oncogene and muscle-specific actin genes. (ebi.ac.uk)
  • These specialized sigma factors bind the promoters of genes appropriate to the environmental conditions, increasing the transcription of those genes. (wikipedia.org)
  • σ 70 (RpoD) - σ A - the "housekeeping" sigma factor or also called as primary sigma factor , transcribes most genes in growing cells. (wikipedia.org)
  • Every cell has a "housekeeping" sigma factor that keeps essential genes and pathways operating. (wikipedia.org)
  • [1] Genes recognized by σ 70 all contain similar promoter consensus sequences consisting of two parts. (wikipedia.org)
  • Mechanistically, the Runx3 - CBFbeta (show CBFB ELISA Kits ) transcription factor complex deployed H3K27me3 to Bcl6 (show BCL6 ELISA Kits ) and Tcf7 (show TCF7 ELISA Kits ) genes to suppress the TFH program. (antibodies-online.com)
  • Two enhancers at the TCRalphadelta locus, the TCR alpha enhancer and the TCR delta enhancer (Edelta), are responsible for orchestrating the distinct developmental programs for V(D)J recombination and transcription of the TCR alpha and delta genes, respectively. (duke.edu)
  • [8] It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). (hitchhikersgui.de)
  • Binding of either TNF-alpha or TNF-beta to TNFR1 initiates a signal transduction pathway that results in the activation of the transduction factor NFkappaB, whose target genes are involved in the regulation of inflammatory responses, and, in certain cells induce apoptosis. (mybiosource.com)
  • t(1;19)(q23;p13.3) TCF3-PBX1 fusion in pre-B-cell ALL t(1;19)(q23;p13.3) translocations fusing the PBX1 and E2A genes occur in approximately a quater of paediatric pre-B cell acute lymphoblastic laeukemias. (cancerindex.org)
  • Lactose is inhibiting the repressor, allowing the RNA polymerase to bind with the promoter and express the genes, which synthesize lactase. (wikipedia.org)
  • In eukaryotes the initiation of transcription of protein encoding genes by polymerase II (Pol II) is modulated by general and specific transcription factors. (embl.de)
  • Here, we show that similarly to its homolog in animal systems, the plant RETINOBLASTOMA-RELATED (RBR) protein regulates the differentiation of meristematic cells at the transition zone by allowing mRNA accumulation of AUXIN RESPONSE FACTOR19 ( ARF19 ), a transcription factor involved in cell differentiation. (plantcell.org)
  • We show that both RBR and the cytokinin-dependent transcription factor ARABIDOPSIS RESPONSE REGULATOR12 are required to activate the transcription of ARF19 , which is involved in promoting cell differentiation and thus root growth. (plantcell.org)
  • Molecular cloning and characterization of PEBP2 beta, the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. (jax.org)
  • The gene is highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit gene. (wikipedia.org)
  • Edelta function depends critically on transcription factors core binding factor (CBF)/polyoma enhancer-binding protein 2 (PEBP2) and c-Myb as measured by transcriptional activation of transiently transfected substrates in Jurkat cells, and by activation of V(D)J recombination within chromatin-integrated substrates in transgenic mice. (duke.edu)
  • Our data indicate that CBF/PEBP2 plays primarily a structural role as it induces a conformational change in the enhanceosome that is associated with augmented binding of c-Myb. (duke.edu)
  • In contrast, c-Myb has no apparent affect on CBF/PEBP2 binding, but is critical for transcriptional activation. (duke.edu)
  • RUNX3 encodes a member of the runt domain-containing family of transcription factors. (antibodies-online.com)
  • In addition, activation of Sirt-1 (a histone deacetylase) by resveratrol induced Sirt-1-p300 association in bone-derived and preosteoblastic cells, leading to deacetylation of RANKL-induced NF-kappaB, inhibition of NF-kappaB transcriptional activation, and osteoclastogenesis. (brad.ac.uk)
  • The study summarizes transcriptional regulation factors interacting and cooperating at promoter regions that regulate gene expression. (biomedcentral.com)
  • Transcriptional regulation factors interacting and cooperating at promoter regions determine the fate of cells and their function. (biomedcentral.com)
  • The knowledge evolve transcription factor binding sites on the promoter will allow the creation of the regulation map of each gene and will enable the recognition of other transcription regulation systems that control gene expression. (biomedcentral.com)
  • The chromatin immunoprecipitation (ChIP) assay was used to assess the direct binding of HIF1α to the Smad7 promoter. (springernature.com)
  • We also show that Cbfa1 regulates positively the activity of its own promoter, which has the highest affinity Cbfa1-binding sites characterized. (tmc.edu)
  • [1] Relative to the DNA base corresponding to the start of the RNA transcript, the consensus promoter sequences are characteristically centered at 10 and 35 nucleotides before the start of transcription (−10 and −35). (wikipedia.org)
  • Identification of IFN regulatory factor-1 binding site in IL-12 p40 gene promoter. (labome.org)
  • The gene can be transcribed from 2 alternative promoters , promoter 1 (distal) or promoter 2 (proximal). (hitchhikersgui.de)
  • The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involve the effects of transcription factor IIA (TFIIA) on the interaction between TATA-binding protein (TBP) and DNA. (nih.gov)
  • A sigma factor is the promoter specificity subunit of eubacterial-type multisubunit RNA polymerases, those whose core subunit composition is often described as alpha(2)-beta-beta-prime. (cathdb.info)
  • Although sigma does not bind DNA on its own, when combined with the core to form the holoenzyme, this binds specifically to promoter sequences, with the sigma factor making sequence specific contacts with the promoter elements. (cathdb.info)
  • Any process involved in the assembly of the RNA polymerase preinitiation complex (PIC) at the core promoter region of a DNA template, resulting in the subsequent synthesis of RNA from that promoter. (cathdb.info)
  • A sigma factors binds RNA polymerase through the alpha subunit and then helps the core enzyme detect or a recognize a specific DNA sequence, this is called a promoter. (wikibooks.org)
  • They also help core RNA polymerase locate the consensus promoter sequences near the beginning of a gene. (wikibooks.org)
  • There is no lactose to inhibit the repressor , so the repressor binds to the operator , which obstructs the RNA polymerase from binding to the promoter and making the mRNA encoding the lactase gene. (wikipedia.org)
  • The promoter is recognized by RNA polymerase and an associated sigma factor , which in turn are often brought to the promoter DNA by an activator protein's binding to its own DNA binding site nearby. (wikipedia.org)
  • The process is more complicated, and at least seven different factors are necessary for the binding of an RNA polymerase II to the promoter. (wikipedia.org)
  • As promoters are typically immediately adjacent to the gene in question, positions in the promoter are designated relative to the transcriptional start site , where transcription of DNA begins for a particular gene (i.e., positions upstream are negative numbers counting back from -1, for example -100 is a position 100 base pairs upstream). (wikipedia.org)
  • There is no such thing as a set of "universal elements" found in every core promoter. (wikipedia.org)
  • Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. (abcam.com)
  • This transcription factor is responsible for the differentiation of precursor cells into osteoblasts and regulates the differentiation of chondrocytes in the growth plate [ 4 ]. (springer.com)
  • Knock-out of the DNA-binding activity results in inhibition of osteoblastic differentiation. (wikipedia.org)
  • Then, we selected positive (30/45 Hz, 1 mT) and negative (7.5 Hz, 1 mT) osteogenic differentiation conditions by quantifying alkaline phosphate (ALP) mRNA expression. (bvsalud.org)
  • Human serum response factor (SRF) is a ubiquitous nuclear protein important for cell proliferation and differentiation. (ebi.ac.uk)
  • A redox factor protein, ref1, is involved in negative gene regulation by extracellular calcium. (labome.org)
  • CCAAT/enhancer binding protein-mediated regulation of TGF? (labome.org)
  • DNA-directed RNA polymerase, omega subunit / Sigma-54 factors family profile. (pdbj.org)
  • The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. (rcpcm.org)
  • Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor. (jax.org)
  • To determine if Cbfa1 plays a role during bone formation we generated transgenic mice overexpressing Cbfa1 DNA-binding domain (DeltaCbfa1) in differentiated osteoblasts only postnatally. (tmc.edu)
  • CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. (abcam.com)
  • These effects were abolished with cotreatment by ACE inhibitors or angiotensin type 1 receptor blockers (ARBs). (hindawi.com)
  • Alkaline phosphatase (ALP) activity was determined, and reverse transcription polymerase chain reaction was used to measure the expressions of runt-related transcription factor 2 (Runx-2), osteocalcin (OCN), bone sialoproteins (BSP), receptor activator of NF-κB ligand (RANKL) and osteoprotegerin (OPG) mRNA. (bvsalud.org)
  • Kikuchi Y, Yasue T, Miyake K, Kimoto M, Takatsu K. CD38 ligation induces tyrosine phosphorylation of Bruton tyrosine kinase and enhanced expression of interleukin 5-receptor alpha chain: synergistic effects with interleukin 5. (labome.org)
  • These data indicate that CD38 ligation increases IL-5 receptor alpha expression and synergizes with IL-5 to enhance Blimp1 expression and IgM synthesis. (labome.org)
  • Interferon-alpha and interleukin-12 are induced differentially by Toll-like receptor 7 ligands in human blood dendritic cell subsets. (labome.org)
  • A discoidin domain receptor 1 knock-out mouse as a novel model for osteoarthritis of the temporomandibular joint. (ox.ac.uk)
  • Discoidin domain receptor 1 (DDR-1)-deficient mice exhibited a high incidence of osteoarthritis (OA) in the temporomandibular joint (TMJ) as early as 9 weeks of age. (ox.ac.uk)
  • Soluble TNF Receptor I (sTNFR1) is capable of inhibiting TNF-alpha and TNF-beta activities by acting as a decoy receptor that serves as a sink for the TNF ligands. (mybiosource.com)
  • In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. (merckmillipore.com)
  • A gene on chromosome 6p21 that encodes a nuclear protein member of the RUNX family of transcription factors with a Runt DNA-binding domain. (thefreedictionary.com)
  • This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. (wikipedia.org)
  • We show that RNT-1, the Caenorhabditis elegans RUNX homolog, is constantly produced and degraded by the ubiquitination-proteasome pathway in the intestine of the nematode. (elsevier.com)
  • RUNX-1 multiprotein complex purification from induced murine megakaryoblastic L8057 cells. (asm.org)
  • A) Metabolic biotin tagging of RUNX-1 in L8057 cells. (asm.org)
  • Schematic diagram (top) shows coexpression of birA and biotin-tagged RUNX-1 in L8057 cells. (asm.org)
  • Western blot (WB) analysis of nuclear extracts from stable L8057 clonal lines expressing birA alone or birA and recombinant-tagged RUNX-1 using anti-RUNX-1 antibody (bottom left) and SA-conjugated HRP (SA-HRP) (bottom right). (asm.org)
  • Two endogenous RUNX-1 isoforms are indicated, with the larger corresponding to murine isoform 3. (asm.org)
  • Recombinant RUNX-1 is indicated running slightly slower than endogenous isoform 3. (asm.org)
  • B) Schematic diagram showing anti-FLAG-SA tandem affinity purification of FLAG-Bio RUNX-1-containing complexes. (asm.org)
  • Physical interaction between RUNX-1 and FLI-1. (asm.org)
  • A) FLI-1 Western blot (WB) analysis of single SA (top)- or tandem anti-FLAG-SA (bottom)-purified FLAG-Bio RUNX-1 complexes from TPA-induced L8057 cells. (asm.org)
  • B) Co-IP of endogenous RUNX-1 and FLI-1 from TPA-induced L8057 cells. (asm.org)
  • Immunoprecipitation with normal rabbit IgG or anti-RUNX-1 antibody, and Western blot with anti-FLI-1 antibody. (asm.org)
  • D) Direct RUNX-1-FLI-1 protein-protein interactions. (asm.org)
  • In vitro transcribed and translated [ 35 S]methionine-labeled ( 35S ) FLI-1, RUNX-1, runt domain, or CBF-β was incubated with uncoupled Sepharose beads or beads coupled with bacterially produced GST, GST-RUNX-1, GST-FLI-1, or GST-runt domain, as indicated. (asm.org)
  • Mapping of RUNX-1 and FLI-1 interaction domains. (asm.org)
  • A) Mapping of FLI-1 domains involved in RUNX-1 interaction. (asm.org)
  • Right) 293T cells were cotransfected with the FLAG-tagged FLI-1 constructs and V5-tagged RUNX-1. (asm.org)
  • They consist of a DNA binding Runx subunit and a non-DNA binding CBFβ sub-unit. (dartmouth.edu)
  • DDR-1-deficient chondrocytes from the TMJ were positively influenced towards chondrogenesis by a three-dimensional matrix combined with a runx-2 knockdown or stimulation with extracellular matrix components, such as nidogen-2. (ox.ac.uk)
  • Distinct roles for c-Myb and core binding factor/polyoma enhancer-binding protein 2 in the assembly and function of a multiprotein complex on the TCR delta enhancer in vivo. (duke.edu)
  • The in-growing blood vessels deliver osteoblastic, osteoclastic, and hematopoietic precursors, which mediate resorption of the cartilaginous template and formation of vascularized bone containing the so-called stromal sinusoids, which provide the microenvironment for hematopoiesis ( 1 ). (pnas.org)
  • The alpha subunit binds DNA and appears to have a role in the development of normal hematopoiesis. (abcam.com)
  • Core-binding factors in hematopoiesis and immune function. (ox.ac.uk)
  • All four subunits are required at one or more stages of hematopoiesis. (ox.ac.uk)
  • Core-binding factors (CBFs) are a small family of transcription factors that play important roles in several developmental processes and in human disease. (dartmouth.edu)
  • Core Binding Factors (CBFs) are members of a small family of transcription factors that play critical roles in a number of mammalian developmental processes. (dartmouth.edu)
  • The MADS-box family of transcription factors. (ebi.ac.uk)
  • Metabolic bone disorder is a common pathological mechanism with genetic components characterized by reduced bone mass and increased risk of fractures [ 1 ]. (hindawi.com)
  • Co-treatment with resveratrol activated the bone transcription factors Cbfa-1 and Sirt-1 and induced the formation of Sirt-1-Cbfa-1 complexes. (brad.ac.uk)
  • Overall, these results demonstrate that resveratrol-activated Sirt-1 plays pivotal roles in regulating the balance between the osteoclastic versus osteoblastic activity result in bone formation in vitro thereby highlighting its therapeutic potential for treating osteoporosis and rheumatoid arthritis-related bone loss. (brad.ac.uk)
  • Core binding factor (CBF) is a heterodimeric transcription factor that is essential for a number of developmental process including hemotopoiesis and bone development. (dartmouth.edu)
  • Mechanical stress is an important factor affecting bone tissue homeostasis. (bvsalud.org)
  • Title: Runt-related transcription factor 1 regulates luteinized hormone-induced prostaglandin-endoperoxide synthase 2 expression in rat periovulatory granulosa cells. (genscript.com)
  • Thus, Txk is expressed on Th1/Th0 cells with the IFN-gamma production and acts as a Th1 cell-specific transcription factor. (labome.org)
  • Moreover, UT-7/EN cells formed cell aggregates as they proliferated, suggesting the formation of cell-cell adhesion. (tamuc.edu)
  • The gene expression profiles of UT-7/Epo and UT-7/EN cells were compared using HumanHT-12 v4 BeadChip array and by validation with qRT-PCR. (tamuc.edu)
  • 0.01) suggested E-v cadherin mediated cell-cell interactions in UT-7/EN cells. (tamuc.edu)
  • 0.01), whereas β-catenin gene expression remained non-significant at 24, 48, and 72 hours relative to un-supplemented UT-7/EN cells. (tamuc.edu)
  • 0.001) relative to Epo un-supplemented UT-7/EN cells. (tamuc.edu)
  • Increased frequencies of myelin oligodendrocyte glycoprotein/MHC class II-binding CD4 cells in patients with multiple sclerosis. (umassmed.edu)
  • Interaction with human PPIA/CYPA protects the virus from restriction by human TRIM5-alpha and from an unknown antiviral activity in human cells. (proteopedia.org)
  • Transforming Growth Factor β Drives Hemogenic Endothelium Programming and the Transition to Hematopoietic Stem Cells. (ox.ac.uk)
  • Here we demonstrate that transforming growth factor β (TGFβ) is required for HSPC specification and that it regulates the expression of the Notch ligand Jagged1a in endothelial cells prior to EHT, in a striking parallel with the epithelial-to-mesenchymal transition (EMT). (ox.ac.uk)
  • To understand the molecular mechanisms for synergy between these transcription factors in the context of chromatin, we used in vivo footprinting to study the requirements for protein binding to Edelta within wild-type and mutant versions of a human TCR delta minilocus in stably transfected Jurkat cells. (duke.edu)
  • Determined by its inhibitory effect of the TNF-alpha mediated cytotoxicity in murine L-929 cells. (mybiosource.com)
  • Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected STAT3 in HeLa and A431 cells. (merckmillipore.com)
  • In the present study, we explored whether, in undifferentiated ARPE-19 retinal cells, a disruption of the VEGF receptors (VEGF-R)/caveolin-1 (Cav-1)/protein kinases pathway could be a target for counteracting VEGF secretion. (bvsalud.org)
  • It is homologous to archaeal transcription factor B and to eukaryotic TFIIB . (wikipedia.org)
  • SRF-like/Type I subfamily of MADS (MCM1, Agamous, Deficiens, and SRF (serum response factor)) box family of eukaryotic transcriptional regulators [ PMID: 7744019 ]. (ebi.ac.uk)
  • A sigma factor ( σ factor ) is a protein needed only for initiation of transcription. (wikipedia.org)
  • [2] The specific sigma factor used to initiate transcription of a given gene will vary, depending on the gene and on the environmental signals needed to initiate transcription of that gene. (wikipedia.org)
  • Selection of promoters by RNA polymerase is dependent on the sigma factor that associates with it. (wikipedia.org)
  • Every molecule of RNA polymerase holoenzyme contains exactly one sigma factor subunit, which in the model bacterium Escherichia coli is one of those listed below. (wikipedia.org)
  • For example, σ 70 is the sigma factor with a molecular weight of 70 kDa . (wikipedia.org)
  • RNA polymerase holoenzyme complex consisting of core RNA polymerase and a sigma factor executes transcription of a DNA template strand. (wikipedia.org)
  • Once initiation of RNA transcription is complete, the sigma factor can leave the complex. (wikipedia.org)
  • [1] In the case of E. coli and other gram-negative rod-shaped bacteria, the "housekeeping" sigma factor is σ 70 . (wikipedia.org)
  • σ 32 (RpoH) - the heat shock sigma factor, it is turned on when the bacteria are exposed to heat. (wikipedia.org)
  • There are also anti-sigma factors that inhibit the function of sigma factors and anti-anti-sigma factors that restore sigma factor function. (wikipedia.org)
  • When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription. (rcpcm.org)
  • A gene on chromosome 21q22.3 that encodes the alpha subunit of core binding factor (CBF), a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. (thefreedictionary.com)
  • This gene encodes a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology. (nih.gov)
  • This gene encodes a member of the E protein (class I) family of helix-loop-helix transcription factors. (cancerindex.org)
  • These transcription factors have specific activator or repressor sequences of corresponding nucleotides that attach to specific promoters and regulate gene expression. (wikipedia.org)
  • Osteogenic marker (for example, runt-related transcription factor 2) expression was higher in the 30/45 Hz condition and lower in the 7.5 Hz condition as compared with the nonstimulated group. (bvsalud.org)
  • A tRNA(3)-Lys binds to the primer-binding site (PBS) situated at the 5'-end of the viral RNA. (proteopedia.org)
  • The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. (wikipedia.org)
  • [1] It is a bacterial transcription initiation factor that enables specific binding of RNA polymerase to gene promoters . (wikipedia.org)
  • Moreover, DNA ChIP analysis reveals that this combination prevents the interaction between AP-1 and vegf-a and vegf-r2 gene promoters. (bvsalud.org)
  • The general transcription factors operate through common promoters elements (such as the TATA box). (embl.de)
  • Gene Ontology (GO) annotations related to this gene include DNA binding transcription factor activity and transcription corepressor activity . (genecards.org)
  • Takeba Y, Nagafuchi H, Takeno M, Kashiwakura J, Suzuki N. Txk, a member of nonreceptor tyrosine kinase of Tec family, acts as a Th1 cell-specific transcription factor and regulates IFN-gamma gene transcription. (labome.org)
  • ChIP-sequencing analysis revealed a feedback loop mechanism of theMAPkinase pathway by the VHP-1 phosphatase in the RNT-1-mediated oxidative stress response. (elsevier.com)
  • It binds in the cytoplasm the human BAF protein which prevent autointegration of the viral genome, and might be included in virions at the ration of zero to 3 BAF dimer per virion. (proteopedia.org)
  • Cleidocranial dysplasia (CCD) is a rare autosomal dominant malformation syndrome, with a prevalence rate of 1:1,000,000 and no correlation with gender or ethnic origin [ 1 ]. (springer.com)
  • Long bones and the axial skeleton in the developing embryo are formed by endochondral ossification, namely, by remodeling of cartilage templates ( 1 ). (pnas.org)
  • We focused on the interactions among mechanical stress, glucose uptake via glucose transporter 1 (Glut1), and the cellular energy sensor sirtuin 1 (SIRT1) in osteoblast energy metabolism, since it has been recognized that SIRT1, an NAD+-dependent deacetylase, may function as a master regulator of the mechanical stress response as well as of cellular energy metabolism (glucose metabolism). (bvsalud.org)
  • Nucleic-acid-binding motif in transcriptional elongation factor TFIIS and RNA polymerases. (embl.de)
  • Use Anti-STAT3 Antibody, clone 18F7.1 (mouse monoclonal antibody) validated in WB, ICC to detect STAT3 also known as Acute-phase response factor, DNA-binding protein APRF, signal transducer & activator of transcription 3. (merckmillipore.com)
  • Catalytic activity: CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). (rcpcm.org)
  • Binds these RNAs through its zinc fingers. (proteopedia.org)
  • Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target. (embl.de)
  • This entry represents a zinc finger motif found in transcription factor IIs (TFIIS). (embl.de)
  • Molecular pathogenesis of core binding factor leukemia: current knowledge and future prospects. (medlineplus.gov)
  • Sigma factors are distinguished by their characteristic molecular weights. (wikipedia.org)
  • Kageyama R, Sasai Y, Nakanishi S. Molecular characterization of transcription factors that bind to the cAMP responsive region of the substance P precursor gene. (labome.org)
  • When the cell wants to transcribe the DNA strand, the "TATA binding protein" (transcription factor) attaches to the TATA box and subsequently helps in getting the RNA polymerase to attach there and begin synthesizing the RNA. (wikibooks.org)
  • May function as a testis specific transcription factor. (nih.gov)
  • Core binding factors are heterodimeric transcription factors involved in diverse developmental processes. (dartmouth.edu)
  • In addition to the highly conserved Runt domain, the AML-1 gene product carries a putative ATP-binding site (GRSGRGKS), and has a C-terminal region rich in proline and serine residues. (wikipedia.org)
  • In parallel, chondrocytes in the central regions direct mineralization of the hypertrophic cartilage by initiating remodeling via the production of specific matrix metalloproteinases (MMP) and attract blood vessels by releasing vascular-endothelial growth factor (VEGF). (pnas.org)
  • Chondrocytes isolated from the TMJs of DDR-1-deficient mice maintained their osteoarthritic characteristics when placed in culture. (ox.ac.uk)
  • Control is complex, with involvement of a multitude of factors, thereby regulating the expression and activity of this gene both temporally and spatially. (nih.gov)
  • Forms the conical core that encapsulates the genomic RNA-nucleocapsid complex in the virion. (uniprot.org)
  • We highlight that Resvega®, a combination of omega-3 fatty acids with an antioxidant, resveratrol, inhibits VEGF-A secretion in vitro by disrupting the dissociation of the VEGF-R2/Cav-1 complex into rafts and subsequently preventing MAPK activation. (bvsalud.org)
  • The first would be the initiation step, during which the RNA polymerase II (RNAPII) binds to the DNA site in order to form a preinitiation complex with other transcriptional factors. (wikibooks.org)
  • Crystal structure of a ternary SAP-1/SRF/c-fos SRE DNA complex. (ebi.ac.uk)