Core Binding Factors: Heterodimeric transcription factors containing a DNA-binding alpha subunits, (CORE BINDING FACTOR ALPHA SUBUNITS), along with a non-DNA-binding beta subunits, CORE BINDING FACTOR BETA SUBUNIT. Core Binding Factor regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor alpha Subunits: A family of transcription factors that bind to the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. Family members contain a highly conserved DNA-binding domain known as the runt domain. They can act as both activators and repressors of expression of GENES involved in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor Alpha 1 Subunit: A transcription factor that dimerizes with CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain and is involved in genetic regulation of skeletal development and CELL DIFFERENTIATION.Core Binding Factor beta Subunit: A non-DNA binding transcription factor that is a subunit of core binding factor. It forms heterodimeric complexes with CORE BINDING FACTOR ALPHA SUBUNITS, and regulates GENETIC TRANSCRIPTION of a variety of GENES involved primarily in CELL DIFFERENTIATION and CELL CYCLE progression.Core Binding Factor Alpha 2 Subunit: A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain. Runx1 is frequently mutated in human LEUKEMIAS.Core Binding Factor Alpha 3 Subunit: A transcription factor that dimerizes with the cofactor CORE BINDING FACTOR BETA SUBUNIT to form core binding factor. It contains a highly conserved DNA-binding domain known as the runt domain.Transcription Factor AP-2: A family of DNA binding proteins that regulate expression of a variety of GENES during CELL DIFFERENTIATION and APOPTOSIS. Family members contain a highly conserved carboxy-terminal basic HELIX-TURN-HELIX MOTIF involved in dimerization and sequence-specific DNA binding.Smooth Muscle Myosins: Myosin type II isoforms found in smooth muscle.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Chromosomes, Human, Pair 16: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.DNA-Binding Proteins: Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.Chromosome Inversion: An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Oncogene Proteins, Fusion: The GENETIC TRANSLATION products of the fusion between an ONCOGENE and another gene. The latter may be of viral or cellular origin.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Chromosomes, Human, Pair 21: A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Osteoblasts: Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.Enhancer Elements, Genetic: Cis-acting DNA sequences which can increase transcription of genes. Enhancers can usually function in either orientation and at various distances from a promoter.Translocation, Genetic: A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.Osteogenesis: The process of bone formation. Histogenesis of bone including ossification.Osteocalcin: Vitamin K-dependent calcium-binding protein synthesized by OSTEOBLASTS and found primarily in BONES. Serum osteocalcin measurements provide a noninvasive specific marker of bone metabolism. The protein contains three residues of the amino acid gamma-carboxyglutamic acid (Gla), which, in the presence of CALCIUM, promotes binding to HYDROXYAPATITE and subsequent accumulation in BONE MATRIX.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.Transforming Growth Factor alpha: An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Tumor Necrosis Factor-alpha: Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.Proto-Oncogene Proteins: Products of proto-oncogenes. Normally they do not have oncogenic or transforming properties, but are involved in the regulation or differentiation of cell growth. They often have protein kinase activity.Cell Line: Established cell cultures that have the potential to propagate indefinitely.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Leukemia, Myeloid: Form of leukemia characterized by an uncontrolled proliferation of the myeloid lineage and their precursors (MYELOID PROGENITOR CELLS) in the bone marrow and other sites.Pol1 Transcription Initiation Complex Proteins: Factors that form a preinitiation complex at promoters that are specifically transcribed by RNA POLYMERASE I.Alkaline Phosphatase: An enzyme that catalyzes the conversion of an orthophosphoric monoester and water to an alcohol and orthophosphate. EC 3.1.3.1.Mesenchymal Stromal Cells: Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.Chondrogenesis: The formation of cartilage. This process is directed by CHONDROCYTES which continually divide and lay down matrix during development. It is sometimes a precursor to OSTEOGENESIS.Mesenchymal Stem Cell Transplantation: Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).Chondrocytes: Polymorphic cells that form cartilage.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Drosophila: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.Drosophila melanogaster: A species of fruit fly much used in genetics because of the large size of its chromosomes.Wheat Germ Agglutinins: Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Triticum: A plant genus of the family POACEAE that is the source of EDIBLE GRAIN. A hybrid with rye (SECALE CEREALE) is called TRITICALE. The seed is ground into FLOUR and used to make BREAD, and is the source of WHEAT GERM AGGLUTININS.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Tyrosine: A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.Phosphoproteins

Cbfa1 isoforms exert functional differences in osteoblast differentiation. (1/863)

Cbfa1 is an essential transcription factor for osteoblast differentiation and bone formation. We investigated functional differences among three isoforms of Cbfa1: Type I (originally reported as Pebp2alphaA by Ogawa et al. (Ogawa, E., Maruyama, M., Kagoshima, H., Inuzuka, M., Lu, J., Satake, M., Shigesada, K., and Ito, Y. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 6859-6863), Type II (originally reported as til-1 by Stewart et al. (Stewart, M., Terry, A., Hu, M., O'Hara, M., Blyth, K., Baxter, E., Cameron, E., Onions, D. E., and Neil, J. C. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 8646-8651), and Type III (originally reported as Osf2/Cbfa1 by Ducy et al. (Ducy, P., Zhang, R., Geoffroy, V., Ridall, A. L., and Karsenty, G. (1997) Cell 89, 747-754). A reverse transcriptase-polymerase chain reaction analysis demonstrated that these isoforms were expressed in adult mouse bones. The transient transfection of Type I or Type II Cbfa1 in a mouse fibroblastic cell line, C3H10T1/2, induced the expression of alkaline phosphatase (ALP) activity. This induction was synergistically enhanced by the co-introduction of Xenopus BMP-4 cDNA. In contrast, the transient transfection of Type III cDNA induced no ALP activity. In C3H10T1/2 cells stably transfected with each isoform of Cbfa1, the gene expression of ALP was also strongly induced in cells transfected with Type I and Type II Cbfa1 but not in cells with Type III Cbfa1. Osteocalcin, osteopontin,and type I collagen gene expressions were induced or up-regulated in all of the cells stably transfected with each isoform of Cbfa1, and Type II transfected cells exhibited the highest expression level of osteocalcin gene. A luciferase reporter gene assay using a 6XOSE2-SV40 promoter (6 tandem binding elements for Cbfa1 ligated in front of the SV40 promoter sequence), a mouse osteocalcin promoter, and a mouse osteopontin promoter revealed the differences in the transcriptional induction of target genes by each Cbfa1 isoform with or without its beta-subunit. These results suggest that all three of the Cbfa1 isoforms used in the present study are involved in the stimulatory action of osteoblast differentiation, but they exert different functions in the process of osteoblast differentiation.  (+info)

Regulation of chondrocyte differentiation by Cbfa1. (2/863)

Cbfa1, a developmentally expressed transcription factor of the runt family, was recently shown to be essential for osteoblast differentiation. We have investigated the role of Cbfa1 in endochondral bone formation using Cbfa1-deficient mice. Histology and in situ hybridization with probes for indian hedgehog (Ihh), collagen type X and osteopontin performed at E13.5, E14.5 and E17.5 demonstrated a lack of hypertrophic chondrocytes in the anlagen of the humerus and the phalanges and a delayed onset of hypertrophy in radius/ulna in Cbfa1-/- mice. Detailed analysis of Cbfa1 expression using whole mount in situ hybridization and a lacZ reporter gene reveled strong expression not only in osteoblasts but also in pre-hypertrophic and hypertrophic chondrocytes. Our studies identify Cbfa1 as a major positive regulator of chondrocyte differentiation.  (+info)

Maturational disturbance of chondrocytes in Cbfa1-deficient mice. (3/863)

Cbfa1, a transcription factor that belongs to the runt-domain gene family, plays an essential role in osteogenesis. Cbfa1-deficient mice completely lacked both intramembranous and endochondral ossification, owing to the maturational arrest of osteoblasts, indicating that Cbfa1 has a fundamental role in osteoblast differentiation. However, Cbfa1 was also expressed in chondrocytes, and its expression was increased according to the maturation of chondrocytes. Terminal hypertrophic chondrocytes expressed Cbfa1 extensively. The significant expression of Cbfa1 in hypertrophic chondrocytes was first detected at embryonic day 13.5 (E13.5), and its expression in hypertrophic chondrocytes was most prominent at E14.5-16.5. In Cbfa1-deficient mice, whose entire skeleton was composed of cartilage, the chondrocyte differentiation was disturbed. Calcification of cartilage occurred in the restricted parts of skeletons, including tibia, fibula, radius, and ulna. Type X collagen, BMP6, and Indian hedgehog were expressed in their hypertrophic chondrocytes. However, osteopontin, bone sialoprotein, and collagenase 3 were not expressed at all, indicating that they are directly regulated by Cbfa1 in the terminal hypertrophic chondrocytes. Chondrocyte differentiation was severely disturbed in the rest of the skeleton. The expression of PTH/PTHrP receptor, Indian hedgehog, type X collagen, and BMP6 was not detected in humerus and femur, indicating that chondrocyte differentiation was blocked before prehypertrophic chondrocytes. These findings demonstrate that Cbfa1 is an important factor for chondrocyte differentiation.  (+info)

A Cbfa1-dependent genetic pathway controls bone formation beyond embryonic development. (4/863)

The molecular mechanisms controlling bone extracellular matrix (ECM) deposition by differentiated osteoblasts in postnatal life, called hereafter bone formation, are unknown. This contrasts with the growing knowledge about the genetic control of osteoblast differentiation during embryonic development. Cbfa1, a transcriptional activator of osteoblast differentiation during embryonic development, is also expressed in differentiated osteoblasts postnatally. The perinatal lethality occurring in Cbfa1-deficient mice has prevented so far the study of its function after birth. To determine if Cbfa1 plays a role during bone formation we generated transgenic mice overexpressing Cbfa1 DNA-binding domain (DeltaCbfa1) in differentiated osteoblasts only postnatally. DeltaCbfa1 has a higher affinity for DNA than Cbfa1 itself, has no transcriptional activity on its own, and can act in a dominant-negative manner in DNA cotransfection assays. DeltaCbfa1-expressing mice have a normal skeleton at birth but develop an osteopenic phenotype thereafter. Dynamic histomorphometric studies show that this phenotype is caused by a major decrease in the bone formation rate in the face of a normal number of osteoblasts thus indicating that once osteoblasts are differentiated Cbfa1 regulates their function. Molecular analyses reveal that the expression of the genes expressed in osteoblasts and encoding bone ECM proteins is nearly abolished in transgenic mice, and ex vivo assays demonstrated that DeltaCbfa1-expressing osteoblasts were less active than wild-type osteoblasts. We also show that Cbfa1 regulates positively the activity of its own promoter, which has the highest affinity Cbfa1-binding sites characterized. This study demonstrates that beyond its differentiation function Cbfa1 is the first transcriptional activator of bone formation identified to date and illustrates that developmentally important genes control physiological processes postnatally.  (+info)

Collagenase 3 is a target of Cbfa1, a transcription factor of the runt gene family involved in bone formation. (5/863)

Collagenase 3 (MMP-13) is a recently identified member of the matrix metalloproteinase (MMP) gene family that is expressed at high levels in diverse human carcinomas and in articular cartilage from arthritic patients. In addition to its expression in pathological conditions, collagenase 3 has been detected in osteoblasts and hypertrophic chondrocytes during fetal ossification. In this work, we have evaluated the possibility that Cbfa1 (core binding factor 1), a transcription factor playing a major role in the expression of osteoblastic specific genes, is involved in the expression of collagenase 3 during bone formation. We have functionally characterized a Cbfa motif present in the promoter region of collagenase 3 gene and demonstrated, by cotransfection experiments and gel mobility shift assays, that this element is involved in the inducibility of the collagenase 3 promoter by Cbfa1 in osteoblastic and chondrocytic cells. Furthermore, overexpression of Cbfa1 in osteoblastic cells unable to produce collagenase 3 leads to the expression of this gene after stimulation with transforming growth factor beta. Finally, we show that mutant mice deficient in Cbfa1, lacking mature osteoblasts but containing hypertrophic chondrocytes which are also a major source of collagenase 3, do not express this protease during fetal development. These results provide in vivo evidence that collagenase 3 is a target of the transcriptional activator Cbfa1 in these cells. On the basis of these transcriptional regulation studies, together with the potent proteolytic activity of collagenase 3 on diverse collagenous and noncollagenous bone and cartilage components, we proposed that this enzyme may play a key role in the process of bone formation and remodeling.  (+info)

Cbfa1 is required for epithelial-mesenchymal interactions regulating tooth development in mice. (6/863)

Osteoblasts and odontoblasts, cells that are responsible for the formation of bone and dentin matrices respectively, share several molecular characteristics. Recently, Cbfa1 was shown to be a critical transcriptional regulator of osteoblast differentiation. Mutations in this gene cause cleidocranial dysplasia (CCD), an autosomal dominant disorder in humans and mice characterized by defective bone formation. CCD also results in dental defects that include supernumerary teeth and delayed eruption of permanent dentition. The dental abnormalities in CCD suggest an important role for this molecule in the formation of dentition. Here we describe results of studies aimed at understanding the functions of Cbfa1 in tooth formation. RT-PCR and in situ hybridization analyses show that Cbfa1 has a unique expression pattern in dental mesenchyme from the bud to early bell stages during active epithelial morphogenesis. Unlike that observed in osteoblast differentiation, Cbfa1 is downregulated in fully differentiated odontoblasts and is surprisingly expressed in ectodermally derived ameloblasts during the maturation phase of enamel formation. The role of Cbfa1 in tooth morphogenesis is further illustrated by the misshapen and severely hypoplastic tooth organs in Cbfa1-/- mice. These tooth organs lacked overt odontoblast and ameloblast differentiation and normal dentin and enamel matrices. Epithelial-mesenchymal recombinants demonstrate that dental epithelium regulates mesenchymal Cbfa1 expression during the bud and cap stages and that these effects are mimicked by the FGFs but not by the BMPs as shown by our bead implantation assays. We propose that Cbfa1 regulates the expression of molecules in mesenchyme that act reciprocally on dental epithelium to control its growth and differentiation. Taken together, our data indicate a non-redundant role for Cbfa1 in tooth development that may be distinct from that in bone formation. In odontogenesis, Cbfa1 is not involved in the early signaling networks regulating tooth initiation and early morphogenesis but regulates key epithelial-mesenchymal interactions that control advancing morphogenesis and histodifferentiation of the epithelial enamel organ.  (+info)

Dexamethasone enhances In vitro vascular calcification by promoting osteoblastic differentiation of vascular smooth muscle cells. (7/863)

Vascular calcification is often associated with atherosclerotic lesions. Moreover, the process of atherosclerotic calcification has several features similar to the mineralization of skeletal tissue. Therefore, we hypothesized that vascular smooth muscle cells might acquire osteoblastic characteristics during the development of atherosclerotic lesions. In the present study, we investigated the effect of dexamethasone (Dex), which is well known to be a potent stimulator of osteoblastic differentiation in vitro, on vascular calcification by using an in vitro calcification model. We demonstrated that Dex increased bovine vascular smooth muscle cell (BVSMC) calcification in a dose- and time-dependent manner. Dex also enhanced several phenotypic markers of osteoblasts, such as alkaline phosphatase activity, procollagen type I carboxy-terminal peptide production, and cAMP responses to parathyroid hormone in BVSMCs. We also examined the effects of Dex on human osteoblast-like (Saos-2) cells and compared its effects on BVSMCs and Saos-2 cells. The effects of Dex on alkaline phosphatase activity and the cAMP response to parathyroid hormone in BVSMCs were less prominent than those in Saos-2 cells. Interestingly, we detected that Osf2/Cbfa1, a key transcription factor in osteoblastic differentiation, was expressed in both BVSMCs and Saos-2 cells and that Dex increased the gene expression of both transcription factors. These findings suggest that Dex may enhance osteoblastic differentiation of BVSMCs in vitro.  (+info)

Does adult fracture repair recapitulate embryonic skeletal formation? (8/863)

Bone formation is a continuous process that begins during fetal development and persists throughout life as a remodeling process. In the event of injury, bones heal by generating new bone rather than scar tissue; thus, it can accurately be described as a regenerative process. To elucidate the extent to which fetal skeletal development and skeletal regeneration are similar, we performed a series of detailed expression analyses using a number of genes that regulate key stages of endochondral ossification. They included genes in the indian hedgehog (ihh) and core binding factor 1 (cbfa1) pathways, and genes associated with extracellular matrix remodeling and vascular invasion including vascular endothelial growth factor (VEGF) and matrix metalloproteinase 13 (mmp13). Our analyses suggested that even at the earliest stages of mesenchymal cell condensation, chondrocyte (ihh, cbfa1 and collagen type II-positive) and perichondrial (gli1 and osteocalcin-positive) cell populations were already specified. As chondrocytes matured, they continued to express cbfa1 and ihh whereas cbfa1, osteocalcin and gli1 persisted in presumptive periosteal cells. Later, VEGF and mmp13 transcripts were abundant in chondrocytes as they underwent hypertrophy and terminal differentiation. Based on these expression patterns and available genetic data, we propose a model where Ihh and Cbfa1, together with Gli1 and Osteocalcin participate in establishing reciprocal signal site of injury. The persistence of cbfa1 and ihh, and their targets osteocalcin and gli1, in the callus suggests comparable processes of chondrocyte maturation and specification of a neo-perichondrium occur following injury. VEGF and mmp13 are expressed during the later stages of healing, coincident with the onset of vascularization of the callus and subsequent ossification. Taken together, these data suggest the genetic mechanisms regulating fetal skeletogenesis also regulate adult skeletal regeneration, and point to important regulators of angiogenesis and ossification in bone regeneration.  (+info)

TY - JOUR. T1 - Loss of runt-related transcription factor 3 induces gemcitabine resistance in pancreatic cancer. AU - Horiguchi, Shigeru. AU - Shiraha, Hidenori. AU - Nagahara, Teruya. AU - Kataoka, Jyunnro. AU - Iwamuro, Masaya. AU - Matsubara, Minoru. AU - Nishina, Shinichi. AU - Kato, Hironari. AU - Takaki, Akinobu. AU - Nouso, Kazuhiro. AU - Tanaka, Takehiro. AU - Ichimura, Koichi. AU - Yagi, Takahito. AU - Yamamoto, Kazuhide. PY - 2013/8. Y1 - 2013/8. N2 - Background & Aim: Runt-related transcription factor 3 (RUNX3) is a tumor suppressor gene that is expressed in gastric and other cancers including pancreatic cancer. However, the precise function of RUNX3 in pancreatic cancer has not been fully elucidated. In this study, we aimed to determine the effect of decreased RUNX3 expression in pancreatic cancer. Methods: This study included 36 patients with primary pancreatic cancer, who had undergone pancreaticoduodenectomy. All patients were treated with 1000mg/m2 gemcitabine after the surgery. ...
Runt-related transcription factor 1 (RUNX1) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters and can accelerate apoptosis in various tumors. However, the regulatory mechanisms underlying RUNX1 expression in neuroblastoma (NB), a highly malignant tumor in childhood, remain largely unclear. In this study, we aimed to assess the role of RUNX1 in NB and to reveal the underlying mechanisms that may contribute to finding a potential therapeutics strategy against NB. Growth, invasion, metastasis and angiogenesis were assessed using Cell Counting Kit-8 (CCK-8) immunocytochemistry, and studies involving soft agar, cell invasion, tube formation and whole animals. The levels of expression were measured using real-time quantitative PCR for RNA, Western blot and immunostaining analyses for proteins. Luciferase reporter and chromatin immunoprecipitation assays indicated that RUNX1 directly binds within the BIRC5, CSF2RB and NFKBIA promoter regions to facilitate
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Objective: To evaluate the kinetics of bone formation of Pool 7 EMD, by concentration, on the amount of marrow formation and study factors relating to angiogenesis associated with EMD-induced bone formation. Methods: Fractionated commercial Emdogain® (EMD) was obtained from Straumann International. 60 CDI 7 day old outbred mice were injected using 50 μl aliquots of 10 µg EMD, 10 µg Pool 7 EMD, 2 µg TGF-β (positive control) or phosphate buffered saline (PBS, negative control) delivered in a calvarial injection model. Injections were done for 5 consecutive days. The animals were sacrificed on days 6, 8, 12, 16 and 21 with 6 animals per factor for each time point and were prepared for histological evaluation. Nikon NIS Elements software was used to analyze vascular growth and new bone formation over the five time intervals at 20x magnification. Immunocytochemistry (IHC) was used to identify the kinetics of osteogenesis by evaluating the osteoblast transcription factor Osterix, and the ...
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Our previous reports indicated that (+)-cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) by activating vitamin D receptor (VDR). However, whether and how miRNAs modulate osteogenic differentiation induced by (+)-cholesten-3-one have not been explored. In this study, miRNA array profiling and further validation by quantitative real-time PCR revealed that miR-351 was downregulated during (+)-cholesten-3-one-induced osteogenic differentiation of MSCs. Overexpression of miR-351 by miR-351 precursor transfection markedly inhibited the expression of osteoblast-specific genes, such as alkaline phosphatase (ALP), collagen type II, osteopontin (OPN), and runt-related transcription factor 2 (RUNX2), which consequently decreased a number of calcium mineralized nodules ...
van der Deen M, Taipaleenmaki H, Zhang Y, Teplyuk NM, Gupta A, Cinghu S, Shogren K, Maran A, Yaszemski MJ, Ling L, Cool SM, Leong DT, Dierkes C, Zustin J, Salto-Tellez M, Ito Y, Bae SC, Zielenska M, Squire JA, Lian JB, Stein JL, Zambetti GP, Jones SN, Galindo M, Hesse E, Stein GS, van Wijnen AJ. MicroRNA-34c inversely couples the biological functions of the runt-related transcription factor RUNX2 and the tumor suppressor p53 in osteosarcoma. J Biol Chem. 2013 Jul 19; 288(29):21307-19. Epub 2013 May 29 ...
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RUNX1 antibody (runt-related transcription factor 1) for WB. Anti-RUNX1 pAb (GTX11903) is tested in Human, Mouse samples. 100% Ab-Assurance.
RUNX1 antibody (runt-related transcription factor 1) for ICC/IF, WB. Anti-RUNX1 pAb (GTX129100) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
SOUTH SAN FRANCISCO, CA and THOUSAND OAKS, CA, May 26, 2009 (MARKET WIRE via COMTEX) -- Cytokinetics Incorporated (NASDAQ: CYTK) and Amgen Inc. (NASDAQ: AMGN) today announced that Amgen has exercised its option to obtain an exclusive license, worldwide (excluding Japan), to Cytokinetics cardiac contractility program. The license includes CK-1827452, a novel cardiac myosin activator being developed for the treatment of heart failure. Under the terms of the companies 2006 collaboration and option agreement, Amgen has agreed to pay Cytokinetics a non-refundable exercise fee of $50 million and has assumed responsibility for development and commercialization of CK-1827452 and related compounds, at its expense, subject to specified development and commercial participation rights of Cytokinetics. "After reviewing the data from the CK-1827452 clinical trials, we are excited about the opportunity to advance this molecule," said Amgen Executive Vice President for Research and Development, Roger M. ...
The Author(s) 2014. The attached file is reproduced here in accordance with the copyright policy of the publisher. For information about this conference please refer to the conferences website or contact the authors ...
Autor: Otto, Florian et al.; Genre: Zeitschriftenartikel; Im Druck veröffentlicht: 2002-02-13; Keywords: cleidocranial dysplasia; CCD; transcription factor; core binding factor; runt domain; RUNX2; CBFA1; differentiation; osteoblast; Titel: Mutations in the RUNX2 gene in patients with cleidocranial dysplasia
The t(8;21) acute myeloid leukemia (AML)-associated oncoprotein AML1-ETO disrupts normal hematopoietic differentiation. Here, we have investigated its effects on the transcriptome and epigenome in t(8,21) patient cells. AML1-ETO binding was found at promoter regions of active genes with high levels of histone acetylation but also at distal elements characterized by low acetylation levels and binding of the hematopoietic transcription factors LYL1 and LMO2. In contrast, ERG, FLI1, TAL1, and RUNX1 bind at all AML1-ETO-occupied regulatory regions, including those of the AML1-ETO gene itself, suggesting their involvement in regulating AML1-ETO expression levels. While expression of AML1-ETO in myeloid differentiated induced pluripotent stem cells (iPSCs) induces leukemic characteristics, overexpression increases cell death. We find that expression of wild-type transcription factors RUNX1 and ERG in AML is required to prevent this oncogene overexpression. Together our results show that the interplay ...
The Runx2 transcription factor is critical for commitment to the osteoblast lineage. However, its role in committed osteoblasts and its functions during postnatal skeletogenesis remain unclear. We established a Runx2-floxed line with insertion of loxP sites around exon 8 of the Runx2 gene. Runx2 protein lacking the region encoded by exon 8 is imported into the nucleus and binds target DNA, but exhibits diminished transcriptional activity. We specifically deleted the Runx2 gene in committed osteoblasts using 2.3kb col1a-Cre transgenic mice. Surprisingly, the homozygous Runx2 mutant mice were born alive. The Runx2 heterozygous and homozygous null were grossly indistinguishable from wild-type littermates at birth. Runx2 deficiency did not alter proliferative capacity of osteoblasts during embryonic development (E18). Chondrocyte differentiation and cartilage growth in mutants was similar to wild-type mice from birth to 3 months of age. Analysis of the embryonic skeleton revealed poor calcification ...
PHF2라는 단백질이 뼈를 만드는 세포(조골세포)를 활성화시킨다는 사실을 처음으로 규명했다. 조골세포는 Runx2라는 단백질에 의해 분화가 조절된다. 반면, SUV39HI1라는 효소는 Runx2에 메틸기(CH3)를 붙임으로써 Runx2가 기능을 하지 못하게 하는 장식으로 분화를 방해한다. 성장이 끝난 성인들이 더 이상 키가 크지 않는 것도 SUV39HI1 효소 때문이다. 이에 착안해 Runx2에 붙어 있는 메틸기를 제거하는 방안을 연구한 결과, PHF2 단백질이 조골세포 분화를 유도함으로써, 소아의 뼈 발달 과정이나 골절 후 뼈가 새로 형성되는 과정에 작용한다는 것을 증명했다.. PHF2 단백질은 Runx2에 붙어 있는 메틸기를 제거했으며, 이후 본연의 기능을 회복한 Runx2는 조골세포의 분화를 촉진하여 다시 뼈를 만들기 시작했다. 실제 유전자 조작으로 PHF2 단백질이 과발현된 쥐를 만들어 ...
core binding factor alpha: core binding factor plays a key role in several development pathways and in human disease; has been sequenced
Osteoblast differentiation is a pivotal event in bone formation. Runt-related transcription factor-2 (Runx2) is an essential factor required for osteoblast differentiation and bone formation. However, the underlying mechanism of Runx2-regulated osteogenic differentiation is still unclear. Here, we explored the corresponding mechanism using the C2C12/Runx2Dox subline, which expresses Runx2 in response to doxycycline (Dox). We found that Runx2-induced osteogenic differentiation of C2C12 cells results in a sustained decrease in the expression of heparin-binding EGF-like growth factor (HB-EGF), a member of the epidermal growth factor (EGF) family. Forced expression of HB-EGF or treatment with HB-EGF is capable of reducing the expression of alkaline phosphatase (ALP), a defined marker of early osteoblast differentiation. HB-EGF-mediated inhibition of ALP depends upon activation of the EGFR and the downstream extracellular signal-regulated kinase, c-Jun N-terminal kinase mitogen-activated protein kinase
Cleidocranial dysplasia (CCD) is an autosomal dominant skeletal dysplasia. Affected individuals have hypoplastic/aplastic clavicles and multiple dental abnormalities.
Runt-related transcription factor 1 (Runx1), a master regulator of hematopoiesis, is expressed in preosteoclasts. Previously we evaluated the bone phenotype of CD11b-Cre Runx1(fl/fl) mice and demonstrated enhanced osteoclasts and decreased bone mass in males. However, an assessment of the effects of Runx1 deletion in female osteoclast precursors was impossible with this model. Moreover, the role of Runx1 in myeloid cell differentiation into other lineages is unknown. Therefore, we generated LysM-Cre Runx1(fl/fl) mice, which delete Runx1 equally (∼80% deletion) in myeloid precursor cells from both sexes and examined the capacity of these cells to differentiate into osteoclasts and phagocytic and antigen-presenting cells. Both female and male LysM-Cre Runx1(fl/fl) mice had decreased trabecular bone mass (72% decrease in bone volume fraction) and increased osteoclast number (2-3 times) (P < .05) without alteration of osteoblast histomorphometric indices. We also demonstrated that loss of Runx1 in ...
TY - JOUR. T1 - Tyrosine phosphorylation controls Runx2-mediated subnuclear targeting of YAP to repress transcription. AU - Zaidi, Sayyed K.. AU - Sullivan, Andrew J.. AU - Medina, Ricardo. AU - Ito, Yoshiaki. AU - van Wijnen, Andre J. AU - Stein, Janet L.. AU - Lian, Jane B.. AU - Stein, Gary S.. PY - 2004/2/25. Y1 - 2004/2/25. N2 - Src/Yes tyrosine kinase signaling contributes to the regulation of bone homeostasis and inhibits osteoblast activity. Here we show that the endogenous Yes-associated protein (YAP), a mediator of Src/Yes signaling, interacts with the native Runx2 protein, an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. Runx2, through its PY motif, recruits YAP to subnuclear domains in situ and to the osteocalcin (OC) gene promoter in vivo. Inhibition of Src/Yes kinase blocks tyrosine phosphorylation of YAP and dissociates endogenous Runx2-YAP complexes. Consequently, recruitment of the YAP co-repressor to ...
The molecular mechanisms that transduce the osteoblast response to physical forces in the bone microenvironment are poorly understood. Here, we used genetic and pharmacological experiments to determine whether the polycystins PC1 and PC2 (encoded by Pkd1 and Pkd2) and the transcriptional coactivator TAZ form a mechanosensing complex in osteoblasts. Compound-heterozygous mice lacking 1 copy of Pkd1 and Taz exhibited additive decrements in bone mass, impaired osteoblast-mediated bone formation, and enhanced bone marrow fat accumulation. Bone marrow stromal cells and osteoblasts derived from these mice showed impaired osteoblastogenesis and enhanced adipogenesis. Increased extracellular matrix stiffness and application of mechanical stretch to multipotent mesenchymal cells stimulated the nuclear translocation of the PC1 C-terminal tail/TAZ (PC1-CTT/TAZ) complex, leading to increased runt-related transcription factor 2-mediated (Runx2-mediated) osteogenic and decreased PPARγ-dependent adipogenic ...
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Loss of RUNX1/ETO Triggers C/EBPα-Driven Reorganization of the Leukemic Transcriptional Network(A) RUNX1/ETO and CEBPA mRNA expression levels in Kasumi-1 cells
CATAGAGCCA GCGGGCGCGG GCGGGACGGG CGCCCCGCGG CCGGACCCAG CCAGGGCACC ACGCTGCCCG GCCCTGCGCC GCCAGGCACT TCTTTCCGGG ^1 ^11 ^21 ^31 ^41 ^51 ^61 ^71 ^81 ^91 GCTCCTAGGG ACGCCAGAAG GAAGTCAACC TCTGCTGCTT CTCCTTGGCC TGCGTTGGAC CTTCCTTTTT TTGTTGTTTT TTTTTGTTTT TCCCCTTTCT ^101 ^111 ^121 ^131 ^141 ^151 ^161 ^171 ^181 ^191 TCCTTTTGAA TTAACTGGCT TCTTGGCTGG ATGTTTTCAA CTTCTTTCCT GGCTGCGAAC TTTTCCCCAA TTGTTTTCCT TTTACAACAG GGGGAGAAAG ^201 ^211 ^221 ^231 ^241 ^251 ^261 ^271 ^281 ^291 TGCTCTGTGG TCCGAGGCGA GCCGTGAAGT TGCGTGTGCG TGGCAGTGTG CGTGGCAGGA TGTGCGTGCG TGTGTAACCC GAGCCGCCCG ATCTGTTTCG ^301 ^311 ^321 ^331 ^341 ^351 ^361 ^371 ^381 ^391 ATCTGCGCCG CGGAGCCCTC CCTCAAGGCC CGCTCCACCT GCTGCGGTTA CGCGGCGCTC GTGGGTGTTC GTGCCTCGGA GCAGCTAACC GGCGGGTGCT ^401 ^411 ^421 ^431 ^441 ^451 ^461 ^471 ^481 ^491 GGGCGACGGT GGAGGAGTAT CGTCTCGCTG CTGCCCGAGT CAGGGCTGAG TCACCCAGCT GATGTAGACA GTGGCTGCCT TCCGAAGAGT GCGTGTTTGC ^501 ^511 ^521 ^531 ^541 ^551 ^561 ^571 ^581 ^591 ATGTGTGTGA CTCTGCGGCT GCTCAACTCC CAACAAACCA GAGGACCAGC ...
TY - JOUR. T1 - Uremia induces the osteoblast differentiation factor Cbfa1 in human blood vessels. AU - Moe, Sharon. AU - Duan, Danxia. AU - Doehle, Brian P.. AU - ONeill, Kalisha D.. AU - Chen, Xuening (Neal). PY - 2003/3/1. Y1 - 2003/3/1. N2 - Background. Bone matrix proteins are expressed in calcified arteries from dialysis patients, suggesting that vascular smooth muscle cells (VSMCs) may transform to osteoblast-like cells. One of the key transcriptional regulators of osteoblast differentiation is Cbfa1. Thus, we hypothesized that this may be a key factor in arterial calcification. Methods. To test this hypothesis, we examined sections of the inferior epigastric artery from uremic patients for the presence of Cbfa1 and type I collagen and osteopontin by in situ hybridization and immunostaining. We also examined the effect of pooled uremic sera from dialysis patients on the expression of Cbfa1 by reverse transcription-polymerase chain reaction (RT-PCR) in bovine VSMCs in vitro. Results. ...
Looking for online definition of cleidocranial in the Medical Dictionary? cleidocranial explanation free. What is cleidocranial? Meaning of cleidocranial medical term. What does cleidocranial mean?
Osteoporosis is a complex multifactorial disorder of the skeleton. Genetic factors are important in determining peak bone mass and structure, as well as the predisposition to bone deterioration and fragility fractures. Nonetheless, genetic factors alone are not sufficient to explain osteoporosis development and fragility fracture occurrence. Indeed, epigenetic factors, representing a link between individual genetic aspects and environmental influences, are also strongly suspected to be involved in bone biology and osteoporosis. Recently, alterations in epigenetic mechanisms and their activity have been associated with aging. Also, bone metabolism has been demonstrated to be under the control of epigenetic mechanisms. Runt-related transcription factor 2 (RUNX2), the master transcription factor of osteoblast differentiation, has been shown to be regulated by histone deacetylases and microRNAs (miRNAs). Some miRNAs were also proven to have key roles in the regulation of Wnt signalling in osteoblastogenesis
Currently, no model is available to study the cellular and molecular events associated with bone metastases of prostate cancer. This study shows that MDA PCa 2a and MDA PCa 2b cells induce a specific and reproducible increase in osteoblast differentiation and proliferation when the cells share the medium during coculturing. Osteoblast differentiation in this system was associated with up-regulation of the osteoblast-specific transcriptor factor Cbfa1. Moreover, up-regulation of Cbfa1 and Osteocalcin expression was also induced in PMOs by CM produced by MDA PCa 2b cells, suggesting that soluble factors produced by prostate cancer cells promote osteoblast differentiation and that Cbfa1 mediates this effect. To our knowledge, this is the first in vitro model of bone metastasis from prostate cancer that recapitulates the osteoblastic phenotype typical of the disease. These results confirmed in vivo and at the molecular level, suggest that the pathophysiology of osteoblastic bone metastases from ...
Gla-osteocalcin and Glu-osteocalcin can be assayed concurrently with the Mouse Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK127) and Mouse Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK129), respectively. These mouse osteocalcin ELISA kits allow for the relative evaluation of Gla/Glu-osteocalcins, thereby providing a measure of both bone formation and bone resorption.. These kits use a sandwich ELISA strategy to specifically detect the Gla and Glu forms of osteocalcin. The capture antibody is a plate-bound solid-phased rat monoclonal antibody that specifically recognizes the C-terminal region of mouse osteocalcin. This is paired with labeled monoclonal antibodies that are specific for captured Glu-type or Gla-type osteocalcin. Because mouse osteocalcin has C-terminal sequences that differ from other mammals, it is possible to measure mouse osteocalcin without any cross-reaction with bovine antigens. Therefore, the process of osteoblastic cell differentiation can be monitored in ...
Gla-osteocalcin and Glu-osteocalcin can be assayed concurrently with the Mouse Gla-Osteocalcin High Sensitive EIA Kit (Cat. # MK127) and Mouse Glu-Osteocalcin High Sensitive EIA Kit (Cat. # MK129), respectively. These mouse osteocalcin ELISA kits allow for the relative evaluation of Gla/Glu-osteocalcins, thereby providing a measure of both bone formation and bone resorption.. These kits use a sandwich ELISA strategy to specifically detect the Gla and Glu forms of osteocalcin. The capture antibody is a plate-bound solid-phased rat monoclonal antibody that specifically recognizes the C-terminal region of mouse osteocalcin. This is paired with labeled monoclonal antibodies that are specific for captured Glu-type or Gla-type osteocalcin. Because mouse osteocalcin has C-terminal sequences that differ from other mammals, it is possible to measure mouse osteocalcin without any cross-reaction with bovine antigens. Therefore, the process of osteoblastic cell differentiation can be monitored in ...
Osterix, a zinc-finger transcription factor, is required for osteoblast differentiation and new bone formation during embryonic development. The c-Src of tyrosine kinase is involved in a variety of cellular signaling pathways, leading to the induction of DNA synthesis, cell proliferation, and cytoskeletal reorganization. Src activity is tightly regulated and its dysregulation leads to constitutive activation and cellular transformation. The function of Osterix can be also modulated by post-translational modification. But the precise molecular signaling mechanisms between Osterix and c-Src are not known. In this study we investigated the potential regulation of Osterix function by c-Src in osteoblast differentiation. We found that c-Src activation increases protein stability, osteogenic activity and transcriptional activity of Osterix. The siRNA-mediated knockdown of c-Src decreased the protein levels and transcriptional activity of Osterix. Conversely, Src specific inhibitor, SU6656, decreased ...
Breast cancer is one of the most common malignant diseases in women. Epithelial-mesenchymal transition (EMT) has been documented to play an important role in proliferation, invasion and metastasis of tumor cells as well as drug resistance. Even though the signal transducer and activator of transcription 3 (STAT3) is not a master transcription factor of EMT, STAT3 is involved in the regulation of EMT-related gene expression. However, it remains unclear whether targeted inhibitors of STAT3 affect EMT-mediated proliferation, migration, invasion and drug resistance of tumor cells. In this paper, we investigated the effects of STAT3 and its interaction with Twist, a master transcription factor, in EMT program and subsequent changes in proliferation, migration and invasion of breast cancer cells by interfering STAT3 signaling pathway with different strategies such as STAT3 inactivation and STAT3 silencing. Furthermore, we explored the role of inhibiting STAT3 phosphorylation in the EMT regulation of ...
c-Src and IL-6 inhibit osteoblast differentiation and integrate IGFBP5 signalling.: Interleukin-6 (IL-6) and c-Src impair osteoblast maturation in vitro and in
BioAssay record AID 638977 submitted by ChEMBL: Induction of osteoblast differentiation in mouse C2C12 cells assessed as increase in ALP promoter activity measured 48 hrs by luciferase reporter gene analysis.
Engineering surfaces to direct integrin binding and signaling to promote osteoblast differentiation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Mouse Monoclonal Anti-RUNX3/CBFA3 Antibody (2B10E8) [DyLight 488]. Validated: WB, Flow, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
Xp3 (panel A), GZMB (panel B), RUNX3 (panel C) immunostaining. *P,0,05 vs NM; { P,0,05 vs CRC. RUNX3 level in CD8+ T cells coexpressing (white bars) and not
Effects of silica-gentamicin nanohybrids on osteogenic differentiation of human osteoblast-like SaOS-2 cells Wei He,1 Dina A Mosselhy,2,3 Yudong Zheng,1 Qingling Feng,4 Xiaoning Li,4 Xing Yang,4 Lina Yue,1 Simo-Pekka Hannula2 1School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, Peopleâ s Republic of China; 2Department of Chemistry and Materials Science, School of Chemical Engineering, Aalto University, Espoo, Finland; 3Microbiological Unit, Fish Diseases Department, Animal Health Research Institute, Giza, Egypt; 4State Key Laboratory of New Ceramics and Fine Processing, School of Materials Science and Engineering, Tsinghua University, Beijing, Peopleâ s Republic of China Introduction: In recent years, there has been an increasing interest in silica (SiO2) nanoparticles (NPs) as drug delivery systems. This interest is mainly attributed to the ease of their surface functionalization for drug loading. In orthopedic applications, gentamicin-loaded SiO2
Mutations in the coactivator CREB-binding protein (CBP) are a major cause of the human skeletal dysplasia Rubinstein-Taybi syndrome (RTS); however, the mechanism by which these mutations affect skeletal mineralization and patterning is unknown. Here, we report the identification of 3-phosphoinositide-dependent kinase 1 (PDK1) as a key regulator of CBP activity and demonstrate that its functions map to both osteoprogenitor cells and mature osteoblasts. In osteoblasts, PDK1 activated the CREB/CBP complex, which in turn controlled runt-related transcription factor 2 (RUNX2) activation and expression of bone morphogenetic protein 2 (BMP2). These pathways also operated in vivo, as evidenced by recapitulation of RTS spectrum phenotypes with osteoblast-specific Pdk1 deletion in mice (Pdk1osx mice) and by the genetic interactions observed in mice heterozygous for both osteoblast-specific Pdk1 deletion and either Runx2 or Creb deletion. Finally, treatment of Pdk1osx and Cbp+/- embryos with BMPs in utero ...
The heterotopic ossification of muscles, tendons, and ligaments is a common problem faced by orthopaedic surgeons. Runx2/Cbfa1 plays an essential role during the osteoblast differentiation and is considered as a molecular switch in osteoblast biology. RNA interference technology is a powerful tool for silencing endogenous or exogenous genes in mammalian cells. In this study, we investigated the effect of Runx2/Cbfa1-specific siRNA on osteoblast differentiation and mineralization in osteoblastic cells, and then constructed adenovirus containing siRNA against Runx2/Cbfa1 (Ad-Runx2-siRNA) to inhibit the formation of heterotopic ossification induced by BMP4, dernineralized bone matrix, and trauma in animal model. Our results showed that the Runx2/Cbfa1-specific siRNA could inhibit the expression of Runx2/Cbfa1 at the level of mRNA and protein. Analysis of the expression of osteoblast maturation genes including type I collagen, osteopontin, bone sialoprotein, and osteocalcin, alkaline phosphatase ...
Genetic studies show that Msx2 and Dlx5 homeodomain (HD) proteins support skeletal development, but null mutation of the closely related Dlx3 gene results in early embryonic lethality. Here we find that expression of Dlx3 in the mouse embryo is associated with new bone formation and regulation of osteoblast differentiation. Dlx3 is expressed in osteoblasts, and overexpression of Dlx3 in osteoprogenitor cells promotes, while specific knock-down of Dlx3 by RNA interference inhibits, induction of osteogenic markers. We characterized gene regulation by Dlx3 in relation to that of Msx2 and Dlx5 during osteoblast differentiation. Chromatin immunoprecipitation assays revealed a molecular switch in HD protein association with the bone-specific osteocalcin (OC) gene. The transcriptionally repressed OC gene was occupied by Msx2 in proliferating osteoblasts, while Dlx3, Dlx5, and Runx2 were recruited postproliferatively to initiate transcription. Dlx5 occupancy increased over Dlx3 in mature osteoblasts at the
The Runt related transcription factors (RUNX) are recognized as key players in suppressing or promoting tumor growth. RUNX3, a member of this family, is known as a tumor suppressor in many types of cancers, although such a paradigm was challenged by some researchers. The TGF-β pathway governs major upstream signals to activate RUNX3. RUNX3 protein consists of several regions and domains. The Runt domain is a conserved DNA binding domain and is considered as the main part of RUNX proteins since. Herein, we compared the effects of Runt domains and full-Runx3 in cell viability by designing two constructs of Runx3, including N-terminal region and Runt domain. We investigated the effect of full-Runx3, N-t, and RD on growth inhibition in AGS, MCF-7, A549, and HEK293 cell lines which are different in TGF-β sensitivity, in the absence and presence of TGF-β. The full length RUNX3 did not notably inhibit growth of these cell lines while, the N-t and RD truncates showed different trends in these cell lines.
Cell culture experiments have indicated that TGF-β and BMPs have both overlapping and opposing functions in bone formation. On the one hand, TGF-β stimulates the recruitment and proliferation of osteoblast progenitors but inhibits their terminal differentiation; and on the other hand, BMPs cooperate in the former process in addition to promoting osteogenic commitment of MSCs and osteoblast maturation (Alliston et al., 2008). However, albeit informative, these in vitro analyses have used exogenous stimulators or inhibitors of cell signaling and differentiation to infer the dynamics of locally released TGF-β and BMP signals during bone formation. For example, increased differentiation of C2C12 cells treated with BMP4 in the presence of an ALK5 inhibitor was interpreted to imply that endogenous TGF-β activity maintains normal bone mass by restricting the rate of osteoblast maturation through Smad-directed blockade of BMP signaling (Maeda et al., 2004). Similarly, genetic studies in mice have ...
Cellular identity in metazoan organisms is frequently established through lineage-specifying transcription factors, which control their own expression through transcriptional positive feedback, while antagonizing the developmental networks of competing lineages. Here, we have uncovered a distinct positive feedback loop that arises from the reciprocal stabilization of the tyrosine kinase ABL and the transcriptional coactivator TAZ. Moreover, we determined that this loop is required for osteoblast differentiation and embryonic skeletal formation. ABL potentiated the assembly and activation of the RUNX2-TAZ master transcription factor complex that is required for osteoblastogenesis, while antagonizing PPARγ-mediated adipogenesis. ABL also enhanced TAZ nuclear localization and the formation of the TAZ-TEAD complex that is required for osteoblast expansion. Last, we have provided genetic data showing that regulation of the ABL-TAZ amplification loop lies downstream of the adaptor protein 3BP2, which ...
The Runx1-CBFbeta transcription factor is required for the emergence of all definitive hematopoietic cells. It is the earliest specific marker of sites from whi...
Col10a1 promoter activity is up-regulated via RUNX2 binding elements in vitro. (A) Transactivation of Col10a1 via RUNX2-binding A and B elements. The RUNX2 expr
Mouse Monoclonal Anti-RUNX3/CBFA3 Antibody (2B10E8) [Biotin]. Validated: WB, IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed.
Expression of RUNX3 (AML2, CBFA3, PEBP2A3) in vagina tissue. Antibody staining with HPA059006 and CAB025416 in immunohistochemistry.
DLX3 is a novel target of PRKACA, and PRKACA mediates BMP signaling during osteoblast differentiation, at least in part, by phosphorylating DLX3 and modulating the protein stability and function of DLX3 ...
Approximately 40% of patients affected by core binding factor (CBF) acute myeloid leukemia (AML) ultimately die from the disease. Few prognostic markers have been identified. In this study we reviewed 192 patients with core binding factor acute myeloid leukemia (AML), treated with curative intent (age, 15-79 years) in 11 Italian institutions. Overall, 10-year overall survival (OS), disease-free survival (DFS), and event-free survival were 63.9%, 54.8%, and 49.9%, respectively; patients with the t(8;21) and inv(16) chromosomal rearrangements exhibited significant differences at diagnosis. Despite similarly high complete remission (CR) rate, patients with inv(16) experienced superior DFS and a high chance of achieving a second CR, often leading to prolonged OS also after relapse. We found that a complex karyotype (ie, ≥4 cytogenetic anomalies) affected survival; the KIT D816 mutation predicted worse prognosis only in patients with the t(8;21) rearrangement, whereas FLT3 mutations had no ...
Thrombospondin-1 (TSP-1), a matricellular protein widely acclaimed to be involved in the inhibition of angiogenesis and tumorigenesis, is synthesized and secreted by many cell types, including osteoblast and cancer cells. TSP-1 is highly upregulated during early stage of osteogenesis, whereas it inhibits terminal osteoblast differentiation. Expression of TSP-1 is downregulated in cancer cells, and its ectopic expression has been shown to restrain tumor growth. Transcriptional regulation of TSP-1 in osteogenesis and cancer is poorly understood; this prompted us to study its regulation by the two key regulators of the aforementioned processes: Runx2 and Runx3. Through a PCR-based cDNA subtraction technique, we identified and cloned a cDNA fragment for mouse TSP-1, whose expression was dramatically upregulated in response to Runx2 expression in mesenchymal stem cells. Moreover, TSP-1 expression was considerably reduced in the lung of Runx2 knockout mouse. On the other hand, TSP-1 gene expression
New York University biologists captured highly transient interactions between transcription factors-proteins that control gene expression-and target genes in the genome and showed that these typically missed interactions have important practical implications. In a new study published in Nature Communications, the researchers developed a method to capture transient interactions of NLP7, a master transcription factor involved in nitrogen use in plants, revealing that the majority of a plants response to nitrogen is controlled by these short-lived regulatory interactions.. "Our approaches to capturing transient transcription factor-target interactions genome-wide can be applied to validate dynamic interactions of transcription factors for any pathway of interest in agriculture or medicine," said Gloria Coruzzi, Carroll & Milton Petrie Professor in NYUs Department of Biology and Center for Genomics and Systems Biology and the papers senior author.. Dynamic interactions between regulatory proteins ...
The researchers identified the hierarchical tree of coherent gene groups and transcription-factor networks that determine the patterns of genes expressed during brain development. They found that some "master transcription factors" at the top level of the hierarchy regulated the expression of a significant number of gene groups.. The scientists findings can be used for selection of transcription factors that could be targeted in the treatment of specific mental disorders.. "We live in the unique time when huge amounts of data related to genes, DNA, RNA, proteins, and other biological objects have been extracted and stored," said lead author Igor Tsigelny, a research scientist with SDSC as well as with UC San Diegos Moores Cancer Center and its Department of Neurosciences.. "I can compare this time to a situation when the iron ore would be extracted from the soil and stored as piles on the ground. All we need is to transform the data to knowledge, as ore to steel. Only the supercomputers and ...
NEW YORK and MELBOURNE, Australia, Feb. 19, 2014-- Regenerative medicine company Mesoblast Limited today announced that it has been granted a key patent by the European Patent Office covering its proprietary adult Mesenchymal Precursor Cells for use in the treatment of cardiac and vascular conditions.
NEW YORK and MELBOURNE, Australia, Nov. 10, 2017 (GLOBE NEWSWIRE) -- Mesoblast Limited (ASX:MSB) (NASDAQ:MESO) today announced that results from the ...
Expression of CBFA2T3 (ETO2, MTG16, MTGR2, RUNX1T3, ZMYND4) in prostate tissue. Antibody staining with HPA065890 in immunohistochemistry.
Buy our Recombinant Human RUNX2 protein. Ab112259 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA, SDS-PAGE. Abcam provides…
Title fatal reinstall of FM10 on osx10.5.8 Your post i found deinstalling FM10, then reinstalling same copy from same installer cd on osx10.5.8 (on
Croatia Airlines is a mid-sized European network carrier serving 34 routes in 20 countries throughout Europe. Its modern fleet consisting of 12 aircraft (six A319/320 and six Q400) continuously contributes to the development of Croatian tourism. Croatia Airlines also provides charter flights and high quality maintenance support as EASA-authorised MRO. ...
TY - JOUR. T1 - Correlation between genotype and supernumerary tooth formation in cleidocranial dysplasia. AU - Suda, N.. AU - Hattori, M.. AU - Kosaki, Kenjiro. AU - Banshodani, A.. AU - Kozai, K.. AU - Tanimoto, K.. AU - Moriyama, K.. PY - 2010/11. Y1 - 2010/11. N2 - Introduction - Cleidocranial dysplasia (CCD, MIM#119600), for which the responsible gene is RUNX2, is a genetic disorder characterized by hypoplasia or aplasia of the clavicles, patent fontaneles, and a short stature. Supernumerary teeth and delayed eruption and impaction of permanent teeth are frequently associated with CCD. Our previous study reported wide intrafamilial variation in supernumerary tooth formation associated with a mutation in the RUNT-domain of RUNX2, suggesting a low correlation between the genotype and supernumerary tooth formation. To further clarify this point, a more precise evaluation was performed. Design - Gene mutational analysis of nine Japanese individuals with CCD was performed. Dental and skeletal ...
Looking for online definition of core-binding factor, runt domain, alpha subunit 3 in the Medical Dictionary? core-binding factor, runt domain, alpha subunit 3 explanation free. What is core-binding factor, runt domain, alpha subunit 3? Meaning of core-binding factor, runt domain, alpha subunit 3 medical term. What does core-binding factor, runt domain, alpha subunit 3 mean?
Runx1 mediates the development of the granular convoluted tubules in the submandibular glands[1] "The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription factor (RUNX) proteins (RUNX1, 2 and 3). This result clearly demonstrates that the Runx /Cbfb signaling pathway is indispensable in the development of the GCTs. However, it is not clear which of the RUNX proteins plays useful role in the development of the GCTs by activating the Runx /Cbfb signaling pathway. Past studies have revealed that the Runx /Cbfb signaling pathway plays important roles in various aspects of development and homeostatic events. Moreover, the Runx genes have different temporospatial ...
Mitochondrial antioxidant genes are major regulators of oxidative stress in the vasculature. Previous data has shown that reducing antioxidant capacity accelerates atherosclerotic plaque size, however, it is unclear whether reducing mitochondrial antioxidant capacity alters plaque composition. Therefore, we tested the hypothesis that reductions of mitochondrial antioxidant capacity will increase osteogenic markers and intimal plaque calcification in aorta from hypercholesterolemic mice. We used Ldlr-/-ApoB100/100 (LA) mice that were either wild-type (LA-MnSOD+/+) or heterozygous (LA-MnSOD+/-) for manganese superoxide dismutase that were fed a Western Diet (TD88137) for 3 or 6 months. Changes in changes gene expression were assessed in aortic arch using qRT-PCR, plaque calcium levels were examined using histological evaluation from aortic cryosections, and endothelial function was measured using isolated organ bath chambers (ex vivo). While expression of SP7 (an osteogenic transcription factor) ...
Estrogen receptor α (ER α) and androgen receptor (AR) are master transcription factors in the breast and prostate, respectively. They are commonly known in development of sexual characteristics. However, both ERα and AR have been known to be involved in breast cancer (BCa) and prostate cancer (PCa) progression, respectively. The Runx family of transcription factors plays a role in hematopoiesis (Runx1), skeletogenesis (Runx2) and neurogenesis (Runx3). In addition, Runx proteins inhibit cell cycle progression, and have been assigned tumor suppressor roles in various contexts. Because both BCa and PCa cells metastasize to bone at high frequency, investigators have interrogated the possibility that they share characteristics with osteoblasts. Indeed, BCa and PCa cells were found to have "osteomimetic" properties, including expression of Runx2 and Runx2-target genes otherwise expressed by osteoblasts. Provoked by the reported physical interaction between AR and Runx2, we initiated a study to test ...
Background Osteoblast differentiation requires the coordinated stepwise expression of multiple genes. Histone deacetylase inhibitors (HDIs) accelerate the osteoblast differentiation process by...
Root cementum represents one of the crucial prerequisites for an intact fixation in the tooths osseous socket, the alveole. Healthy cementum and its self-repair mechanisms are furthermore vital to a sound parodontium and thus for the entire dental health. Central to the thesis at hand was the collection of human cementoblasts from extracted teeth. These had to be cultivated, immortalized and characterized. The ensuing examination covered an analysis of proteins that are presumed typical for cement and bone. Such proteins include collagen type I and III, osteocalcin, osteopontin, bone sialoprotein and osteonectin. Other findings include both transcription factors RUNX2 and SOX9, both of which are not extensively researched in context with cementum yet. This holds true in particular for SOX9. Even though typical for the chondrogenic cell line, this transcription factor and its interrelation with cementum is not covered in relevant literature. In contrast to this, the literary research has ...
Mutations in several genes in Table 3 have been associated with diseases affecting cognitive capacities. DYRK1A, which lies in the Down syndrome critical region, is thought to underlie some of the cognitive impairment associated with having three copies of chromsome 21 (64). Mutations in NRG3 have been associated with schizophrenia, a condition that has been suggested to affect human-specific cognitive traits (65, 66). Mutations in CADPS2 have been implicated in autism (67), as have mutations in AUTS2 (68). Autism is a developmental disorder of brain function in which social interactions, communication, activity, and interest patterns are affected, as well as cognitive aspects crucial for human sociality and culture (69). It may thus be that multiple genes involved in cognitive development were positively selected during the early history of modern humans.. One gene of interest may be RUNX2 (CBFA1). It is the only gene in the genome known to cause cleidocranial dysplasia, which is characterized ...
This study is examining the appropriate dose and side effects of dasatinib, when it is given with the standard of care chemotherapy for children and adolescents
Abnormal Teeth Symptom Checker: Possible causes include Raine Syndrome & Abnormalities of Size and Form of Teeth & Cleidocranial Dysplasia. Check the full list of possible causes and conditions now! Talk to our Chatbot to narrow down your search.
Further we asked if VLA-4 and VLA-5 integrin upregulation is maintained by RUNX1/ETO in the transformed human leukemia cell line Kasumi-1, derived from a t(8;21)+ AML patient. Kasumi-1 cells, which express RUNX1/ETO and to a lesser extent RUNX1/ETOtr,4 bear high levels of VLA-4 whereas the integrin αL subunit is absent in these cells. We specifically down-regulated RUNX1/ETO via lentivirally delivered shRNA targeting the RUNX1/ETO breakpoint sequences (shRE), which are present in both full length and truncated forms (Online Supplementary Figure S3). At Day 4 after transduction with vectors co-expressing shRE and eGFP, α4, α5 and β1 expression levels were significantly reduced as assessed using flow cytometry, while CXCR4 levels remained unaltered (Figure 1I). Similar results were obtained with NHR2 competitive peptides (N89) (Figure 1J), which also interfere with both RUNX1/ETO forms by disrupting RUNX1/ETO tetramer formation.6 These results suggest that integrin subunit expression remains ...
is a professor of pathology at Karmanos Cancer Center, Wayne State University with a track-record of cancer research for over 30 years. He received his MS and Ph.D. degrees in biochemistry from two of the most premiere institutions in India in 1974 and 1978, respectively. In 1978, he migrated to the United States for his post-doctoral training in molecular biology at Memorial Sloan Kettering Cancer Center in New York, and he served in different capacities in several other institutions prior to arriving at Wayne State University in 1989. His research is focused on understanding the role of a "master" transcription factor, NF-κB, and the regulation of its upstream and downstream signaling molecules in solid tumors. Moreover, his focused research has also been directed toward elucidating the molecular mechanisms of action of "natural agents" and synthetic small molecules for cancer prevention and therapy. He has done a tremendous amount of work in vitro and in vivo, documenting that several ...
Results We compared the percentages of expression of Runx2 into the HD and OA groups: no statistically significant difference in the Runx2 expression after the stimulation with the SP was found in both groups, while a statistically significant increase with IGF-1 and TNF-α in HD group (p: 0.08 and 0.043 respectively). In the OA osteoblasts, we found a tendency to decrease the Runx2 expression with IGF-1 and TNF-α (p: 0.068 for both). The comparison HD vs OA showed a non-statistically significant difference between basal values and after the stimulation with SP (p: 0.086) while we found a statistically significant difference with IGF-1 and TNF-α (p: 0.014 for both). We did not find any influence of gender in Runx2 expression. The immunocytochemistry confirmed the findings of Western Blot analysis. ...
J:89327 Yoshida CA, Yamamoto H, Fujita T, Furuichi T, Ito K, Inoue K, Yamana K, Zanma A, Takada K, Ito Y, Komori T, Runx2 and Runx3 are essential for chondrocyte maturation, and Runx2 regulates limb growth through induction of Indian hedgehog. Genes Dev. 2004 Apr 15;18(8):952-63 ...
This information is intended for physicians and related personnel, who understand that medical information is often imperfect, and must be interpreted in the context of a patients clinical data using reasonable medical judgment. This website should not be used as a substitute for the advice of a licensed physician ...
In the present study, we investigated the effect of Notch signaling on BMP9-induced osteogenic differentiation in MSCs, and the possible mechanism underlying this process. Our findings suggested that Notch signaling can enhance the activity of BMP9 to induce osteogenic differentiation in MSCs, and this effect may be partly mediated by upregulation of ALK2.. BMP9, also called GDF-2, is one of the least studied BMPs (44). Numerous studies have indicated that BMP9 has pivotal biological functions in the areas of liver fibrosis, iron metabolism, cartilage formation and angiopoiesis, and recent studies have shown that BMP9 is the strongest inducer of osteogenic differentiation, which has been regarded as a potential factor in tissue engineering (45). The studies concerning BMP9-induced osteogenesis mechanism are conducive to its application in bone-related diseases. Previous research has indicated that fibroblast growth factor 2 (FGF2) inhibits BMP9-induced osteogenic differentiation by blocking ...
Results IL-6 significantly reduced ALP activity, and expression of osteoblastic gene, Runx2 and osteocalcin, and also inhibited mineralization in a dose-dependent manner, which indicates a negative effect of IL-6 on osteoblast differentiation. Signal transduction analyses by Western blotting demonstrated that IL-6 significantly promoted phosphorylation of ERK and STAT3. The negative effect of IL-6 on ALP activity was restored by inhibition of ERK in a dose-dependent manner. On the other hand, the negative effect of IL-6 on ALP activity was enhanced by inhibition of STAT3. These results indicate that ERK has negative effect on osteoblast differentiation, whereas STAT3 has positive one. Moreover, ERK inhibitor enhanced IL-6-triggered STAT3 phosphorylation, and STAT3 inhibitor enhanced IL-6-triggered ERK phosphorylation, suggesting that ERK and STAT3 signaling pathway negatively regulates each other.. ...
Ca2+ is one of the most important second messengers and regulates many cellular processes (Berridge et al., 2000b), and the CaM pathway is critical for osteoblast differentiation (Zayzafoon, 2006). However, the mechanism for controlling [Ca2+]i during osteoblast differentiation is not yet clear. We found that Panx3 functions as a Ca2+ channel in the ER, through which it regulates [Ca2+]i. C2C12 and calvarial cells express IP3Rs. Both Panx3 and IP3Rs function as ER Ca2+ channels; however, their activation mechanisms are different. We found that the Panx3 ER Ca2+ channel was activated through Akt signaling (Fig. 7 A), distinct from IP3-mediated activation of ubiquitous IP3R ER Ca2+ channels (Mikoshiba, 2007). In addition, siRNA for IP3R3 and 2-APB, which is an inhibitor of IP3R-mediated Ca2+ release, inhibited IP3R ER Ca2+ channel activity, but did not inhibit the Panx3 channel (Fig. 5 A). The role of IP3Rs in osteogenic differentiation is also not yet clear. The inhibition of endogenous Panx3 by ...
Osteogenic lineage commitment is often evaluated by analyzing gene expression. However, many genes are transiently expressed during differentiation. The availability of genes for expression is influenced by epigenetic state, which affects the heterochromatin structure. DNA methylation, a form of epigenetic regulation, is stable and heritable. Therefore, analyzing methylation status may be less temporally dependent and more informative for evaluating lineage commitment. Here we analyzed the effect of mechanical stimulation on osteogenic differentiation by applying fluid shear stress for 24 hr to osteocytes and then applying the osteocyte-conditioned medium (CM) to progenitor cells. We analyzed gene expression and changes in DNA methylation after 24 hr of exposure to the CM using quantitative real-time polymerase chain reaction and bisulfite sequencing. With fluid shear stress stimulation, methylation decreased for both adipogenic and osteogenic markers, which typically increases availability of ...
Osteoporosis is a common disorder which affects up to 30% women and 12% men at some point in life. This mostly age-associated disorder has becoming increasingly a major clinical and public health issue as human lifespan increases. Osteoporosis is characterized by reduced bone mass, alterations in bone micro-architecture, reduced bone strength, and elevated risk of fracture (Kanis, 1994). Although both genetic and environmental factors influence the risk of osteoporosis, it has been shown that familial traits are one of the most important clinical risk factors, suggesting the role of genetic factors. The fundamental pathogenic mechanism underlying this disorder includes, (a) failure to achieve peak bone mass during growth and development, (b) excessive bone resorption, and (c) defects in bone formation. Gene knockouts in mice have demonstrated that transcription factors Runx2 and a downstream factor Osterix (Osx) are essential for osteoblast differentiation and bone formation during development. However,
Previously reported that that FTO-knock out mice display postnatal growth retardation, which manifests as reduced body weight and bone mineral density (Guo et al., 2011); however, the molecular mechanism by which FTO stimulates osteogenic differentiation remains unknown. Here, we demonstrate for the first time that FTO stimulates osteogenic differentiation by inducing mild ER stress. The results of the current study illustrate one mechanism underlying the role of FTO in osteogenic differentiation. A relationship between FTO and AMPK has been previously reported (Pitman et al., 2013; Wu et al., 2017). One study demonstrated that AMPK decreases lipid accumulation in skeletal muscle cells by suppressing FTO expression (Wu et al., 2017). Another study revealed that knockdown of FTO reduces phosphorylation of AMPK in Alzheimers disease (Pitman et al., 2013). The current study demonstrated that activation of AMPK markedly increased expression of FTO and that a positive feedback loop existed between ...
Mutations in human and/or mouse homologs are associated with this disease. Synonyms: cleidocranial dysplasia with micrognathia, absent thumbs, and distal aphalangia; cleidocranial dysplasia-micrognathia-absent thumbs syndrome
We previously published a detailed survey of the spatio‐temporal expression of Runx3 during embryonic development (Levanon et al, 2001). Runx3 expression was examined at embryonic day (E) 10.5 and between E14.5 and E16.5, and compared to the expression pattern of Runx1. Immunohistochemistry (IHC) and knock‐in (KI) β‐galactosidase activity (LacZ staining) were used in parallel throughout this analysis to rigorously determine the expression patterns of the two TFs. Runx3 and Runx1 were readily detected in different compartments of the haematopoietic system and also in the dorsal root ganglia (DRG), epidermal appendages and developing skeletal elements (Levanon et al, 2001). However, regarding epithelia an interesting distinction was noted in the expression pattern of Runx1 and Runx3. While Runx1 was expressed in various epithelia including mucosa of the oesophagus and stomach, the salivary glands ducts and the olfactory and respiratory mucosa, Runx3 expression was undetectable in these ...
The Transcription and Translation Process chapter of this College Biology Help and Review course is the simplest way to master transcription and...
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A central feature of this adaptable, robust microscope range is the stable mechanism which can be adjusted precisely. This is emphasized by the functional and ergonomic design ...
The Runx family of transcription factors supports cell fate determination, cell cycle regulation, global protein synthesis control, and genetic as well as epigenetic regulation of target genes. Runx1, which is essential for hematopoiesis; Runx2, which is required for osteoblast differentiation; and Runx3, which is involved in neurologic and gut development; are expressed in the growth plate during chondrocyte maturation, and in the chondrocytes of permanent cartilage structures. While Runx2 is known to control genes that contribute to chondrocyte hypertrophy, the functions of Runx1 and Runx3 during chondrogenesis and in cartilage tissue have been less well studied. The goals of this project were to characterize expression of Runx proteins in articular cartilage and differentiating chondrocytes and to determine the contribution of Runx1 to osteoarthritis (OA). Here, the expression pattern of Runx1 and Runx2 was characterized in normal bovine articular cartilage. Runx2 is expressed at higher levels in
Here, we demonstrate that COX2 inhibition reduces osteogenic gene induction and AoV calcification in a mouse model of CAVD. COX2 expression is increased in calcified AoVs of klotho-deficient mice and is localized to the VICs before the formation of AoV calcification, which occurs in the context of minimal inflammation. In human CAVD, COX2 expression is increased and is present in regions where the valve leaflet is calcified. In porcine VICs, COX2 mRNA expression is induced upon treatment with osteogenic media, and COX2 inhibition reduces osteogenic gene induction and calcification, supporting a direct role for COX2 in VIC mineralization. Furthermore, COX2 inhibition through genetic or pharmacological manipulation is sufficient to reduce AoV calcification and osteogenic gene expression in klotho-deficient mice. Thus, inhibition of COX2 activity is sufficient to reduce AoV calcification in vivo in mice.. COX2 has not previously been associated with the process of calcification in CAVD, but it is ...
The transcription factor RUNX1/AML1 is an important regulator of hematopoiesis and RUNX1 gene is one of the most frequent target of chromosomal translocations in cells of the myeloid lineage [1]. Interestingly, the RUNX1 gene covers 260 kbp of chromosome 21 but surprisingly, all genomic breakpoints for the leukemia causing translocations (8; 21) and (16;21) are found in intron 5 of the gene [2]. Presently, factors involved in maintaining the structural integrity and/or enhancing susceptibility of these regions to undergo recombination are unknown. Moreover, the breakpoint junctions are devoid of common DNA motifs that can explain the high recombination frequency observed. Interestingly however, topoisomerase II and DNase I hypersensitive sites have been found to correlate with breakpoints suggesting that chromatin organization may be responsible for, or contribute to, chromosomal translocation formation [3, 4]. DNA regions that exhibit DNase I hypersensitivity have been extensively associated ...
Van Braeckel-Budimir, N., Martin, M. D., Hartwig, S. M., Legge, K. L., Badovinac, V. P. & Harty, J. T. (2017). Antigen Exposure History Defines CD8 T Cell Dynamics and Protection during Localized Pulmonary Infections. Frontiers in immunology, 8, 40. PMID: 28191007.. Shan, Q., Zeng, Z., Xing, S., Li, F., Hartwig, S. M., Gullicksrud, J. A., Kurup, S. P., Van Braeckel-Budimir, N., Su, Y., Martin, M. D., Varga, S. M., Taniuchi, I., Harty, J. T., Peng, W., Badovinac, V. P. & Xue, H. H. (2017). The transcription factor Runx3 guards cytotoxic CD8,sup,+,/sup, effector T cells against deviation towards follicular helper T cell lineage. Nature immunology. PMID: 28604718.. Martin, M. D., Shan, Q., Xue, H. H. & Badovinac, V. P. (2017). Time and Antigen-Stimulation History Influence Memory CD8 T Cell Bystander Responses. Frontiers in immunology, 8, 634. PMID: 28642758.. Gullicksrud, J. A., Li, F., Xing, S., Zeng, Z., Peng, W., Badovinac, V. P., Harty, J. T. & Xue, H. H. (2017). Differential Requirements for ...
Anterior Fontanelle Open in Adults & Failure to Thrive Symptom Checker: Possible causes include Pyknodysostosis & Esophagitis & Cleidocranial Dysplasia. Check the full list of possible causes and conditions now! Talk to our Chatbot to narrow down your search.
Gene silencing of noggin by small interfering RNA (siRNA) is a promising approach for the treatment of bone defects, because noggin deactivates bone morphogenetic protein-2 (BMP-2) and suppresses osteogenic differentiation. Here, we demonstrated the silencing of the noggin gene by siRNA polyplexes composed o
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Instructive materials able to drive cells, in particular the differentiation of stem cells toward osteoblastic lineages have been investigated as a promising strategy for bone tissue engineering. Inorganic ions, such as phosphorous, calcium, silicon and strontium, have been used in bone regeneration strategies as instructive ions for material based approaches. The use of effective inorganic ions are being investigated as a promising approach for bone regeneration applications mainly due to the fact that are highly available and cost-effective and thus, reducing the need of using expensive and less stable growth factors. The aim of present study is to investigate the effect of the release of silicon (Si) and calcium (Ca) ions from blend of starch and poly-caprolactone (SPCL) scaffolds on the osteogenic behavior of human adipose stem cells (hASC). The scaffolds were developed by a wet-spinning technique and two different solutions were used as coagulation bath, one containing Ca and Si ions and ...
Background. Members of the Runx gene family encode transcription factors that bind to DNA in a sequence-specific manner. Among the three Runx proteins, Runx2 comprises 607 amino acid (aa) residues, is expressed in bone, and plays crucial roles in osteoblast differentiation and bone development. We examined whether the Runx2 gene is also expressed in testes. Methods. Murine testes from 1-, 2-, 3-, 4-, and 10-week-old male mice of the C57BL/6J strain and W∕Wv strain were used throughout the study. Northern Blot Analyses were performed using extracts form the murine testes. Sequencing of cDNA clones and 5′-rapid amplification of cDNA ends were performed to determine the full length of the transcripts, which revealed that the testicular Runx2 comprises 106 aa residues coding novel protein. Generating an antiserum using the amino-terminal 15 aa of Runx2 (Met1 to Gly15) as an antigen, immunoblot analyses were performed to detect the predicted polypeptide of 106 aa residues with the initiating Met1. With
Controlling and detecting cell differentiation in human mesenchymal stem cells (hMSCs) for regenerative medicine remain challenging at present. Here, we developed multifunctional gold nanoparticles (AuNPs) to control and detect osteogenic differentiation in human mesenchymal stem cells (hMSCs) in real-time. The pol Journal of Materials Chemistry B HOT Papers
Sigma-Aldrich offers abstracts and full-text articles by [Jongwon Lee, Bang Ung Youn, Kabsun Kim, Jung Ha Kim, Da-Hye Lee, Semun Seong, Inyoung Kim, Seung-Hee Han, Xiangguo Che, Je-Yong Choi, Yong-Wook Park, Hyun Kook, Kyung Keun Kim, Dae-Sik Lim, Nacksung Kim].
Complete information for CBFB gene (Protein Coding), Core-Binding Factor Beta Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that ... The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants ... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for ... The phosphorylation state of Runx2 also mediates it's DNA-binding activity. Interestingly, the Runx2 DNA-binding activity is ...
... core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5'-pygpyggt-3', of a number of enhancers and ... The protein is a heterodimer of alpha- and beta-subunits. The alpha-subunit binds DNA as a monomer, and appears to have a role ... highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit ... The region of shared similarity, known as the Runt domain, is responsible for DNA-binding and protein-protein interaction. In ...
... or core-binding factor subunit alpha-2 (CBFA2) is a protein that in humans is encoded by the RUNX1 gene. RUNX1 is a ... It belongs to the Runt-related transcription factor (RUNX) family of genes which are also called core binding factor-α (CBFα). ... but its DNA binding affinity is enhanced by 10 fold if it heterodimerises with the core binding factor β (CBFβ), also via the ... RUNX can behave as heterodimeric transcription factors collectively called the core binding factors (CBFs). The consensus ...
Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. It was previously believed that the RNA ... The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) ... Sigma factors in E. coli:. *σ70(RpoD) - σA - the "housekeeping" sigma factor or also called as primary sigma factor, ...
Due to the higher expression, the factor will bind with a high probability to the polymerase-core-enzyme. Doing so, other ... subunits) binds a sigma factor to form a complex called the RNA polymerase holoenzyme. It was previously believed that the RNA ... The core RNA polymerase (consisting of 2 alpha (α), 1 beta (β), 1 beta-prime (β'), and 1 omega (ω) ... Different sigma factors are utilized under different environmental conditions. These specialized sigma factors bind the ...
This gene encodes one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several ... The protein complex that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to ... "Induced alpha helix in the VP16 activation domain upon binding to a human TAF". Science. 277 (5330): 1310-3. doi:10.1126/ ... TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 32kDa, also known as TAF9, is a protein that in ...
DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. doi:10.1126/science.8248780. PMID 8248780 ... RNA polymerase holoenzymes containing other sigma factors recognize different core promoter sequences. <-- upstream downstream ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... "Nuclear factor-kappaB-dependent induction of interleukin-8 gene expression by tumor necrosis factor alpha: evidence for an ...
Chen Y; Le Cahérec F; Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... This gene encodes an alpha subunit of the heteromeric SEC61 complex, which also contains beta and gamma subunits. GRCh38: ... Knight BC; High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... Protein transport protein Sec61 subunit alpha isoform 1 is a protein that in humans is encoded by the SEC61A1 gene. The protein ...
The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ... Transcription initiation factor TFIID subunit 11 also known as TAFII28, is a protein that in humans is encoded by the TAF11 ... The conserved region contains four alpha helices and three loops arranged as in histone H3. TAF11 has been shown to interact ... In molecular biology, TAFII28 refers to the TATA box binding protein associated factor. Together with the TATA-binding protein ...
DNA binding by the alpha subunit of RNA polymerase". Science. 262 (5138): 1407-1413. Bibcode:1993Sci...262.1407R. doi:10.1126/ ... "New core promoter element in RNA polymerase II-dependent transcription: sequence-specific DNA binding by transcription factor ... In the case of a transcription factor binding site, there may be a single sequence that binds the protein most strongly under ... An example is the E-box (sequence CACGTG), which binds transcription factors in the basic helix-loop-helix (bHLH) family (e.g. ...
... subunit of 150 kDa, a beta prime subunit (β′) of 155 kDa, and a small omega (ω) subunit. A sigma (σ) factor binds to the core, ... RNA polymerase "core" from E. coli consists of five subunits: two alpha (α) subunits of 36 kDa, a beta (β) ... The core enzyme has five subunits (~400 kDa):[23] *β': The β' subunit is the largest subunit, and is encoded by the rpoC gene.[ ... In order to bind promoters, RNAP core associates with the transcription initiation factor sigma (σ) to form RNA polymerase ...
... it allosterically enhances DNA binding by the alpha subunit as the complex binds to the core site of various enhancers and ... Core-binding factor subunit beta is a protein that in humans is encoded by the CBFB gene. The protein encoded by this gene is ... "Entrez Gene: CBFB core-binding factor, beta subunit". The Cancer Genome Atlas Network (2012). "Comprehensive molecular ... the beta subunit of a heterodimeric core-binding transcription factor belonging to the PEBP2/CBF transcription factor family ...
This complex consists of three membrane proteins- alpha, beta, and gamma. This gene encodes the beta-subunit protein. The Sec61 ... Chen Y, Le Cahérec F, Chuck SL (1998). "Calnexin and other factors that alter translocation affect the rapid binding of ... Knight BC, High S (1998). "Membrane integration of Sec61alpha: a core component of the endoplasmic reticulum translocation ... 1999). "A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61beta subunit". FEBS ...
"The alpha-like RNA polymerase II core subunit 3 (RPB3) is involved in tissue-specific transcription and muscle differentiation ... cooperation with promoter-bound activator domains and binding to TFIIB". J. Mol. Biol. 261 (5): 599-606. doi:10.1006/jmbi. ... POLR2J has been shown to interact with: Apoptosis antagonizing transcription factor, POLR2C, and SATB1. GRCh38: Ensembl release ... The product of this gene exists as a heterodimer with another polymerase subunit; together they form a core subassembly unit of ...
The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position ... "Structure-function analysis of the estrogen receptor alpha corepressor scaffold attachment factor-B1: identification of a ... This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and ... TATA-binding protein-associated factor 2N is a protein that in humans is encoded by the TAF15 gene. Initiation of transcription ...
In normoxia, HIF alpha subunits are marked for the ubiquitin-proteasome degradation pathway through hydroxylation of proline- ... "Structural basis for binding of hypoxia-inducible factor to the oxygen-sensing prolyl hydroxylases". Structure. 17 (7): 981-9. ... The catalytic domain consists of a double-stranded β-helix core that is stabilized by three α-helices packed along the major β- ... The enzyme has a high affinity for iron(II) and 2-oxoglutarate, and forms a long-lived complex with these factors. It has been ...
TATA-box binding protein), the subunit of the general transcription factor TFIID, than polymerase IIO form. The form polymerase ... RPB3 (POLR2C) - the third-largest subunit. Exists as a heterodimer with another polymerase subunit, POLR2J forming a core ... Alpha-Amanitin is a highly poisonous substance found in many mushrooms. The mushroom poison has different effects on the each ... In combination with several other polymerase subunits, the RPB1 subunit forms the DNA binding domain of the polymerase, a ...
"Multidomain organization of eukaryotic guanine nucleotide exchange translation initiation factor eIF-2B subunits revealed by ... The structure can be divided into a structural C-terminal core onto which the two N-terminal helices are attached. The core ... The W2 domain has a globular fold and is exclusively composed out of alpha-helices. ... the eIF-W2 domain functions as the binding site for Mnk eIF4E kinase, an enzyme that phosphorylates eukaryotic initiation ...
The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are ... It also binds closely to the E3 ubiquitin ligase MDM2, which is a regulator of the degradation of p53 and retinoblastoma ... Accordingly, gene expression by degradation of transcription factors, such as p53, c-Jun, c-Fos, NF-κB, c-Myc, HIF-1α, MATα2, ... which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes ...
It belongs to the thioredoxin superfamily fold which is defined by a beta-sheet core surrounded by alpha-helices. The active ... This two-subunit enzyme produces resistance to arsenite and antimonite. Arsenate, however, must first be reduced to arsenite ... Li S, Rosen BP, Borges-Walmsley MI, Walmsley AR (July 2002). "Evidence for cooperativity between the four binding sites of ... The arsC family also comprises the Spx proteins which are Gram-positive bacterial transcription factors that regulate the ...
Ruediger R, Fields K, Walter G (1999). "Binding specificity of protein phosphatase 2A core enzyme for regulatory B subunits and ... 1990). "alpha- and beta-forms of the 65-kDa subunit of protein phosphatase 2A have a similar 39 amino acid repeating structure ... Hong Y, Sarge KD (1999). "Regulation of protein phosphatase 2A activity by heat shock transcription factor 2". J. Biol. Chem. ... It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, ...
They are composed of a C-terminal ligand-binding region, a core DNA-binding domain (DBD) and an N-terminal domain that contains ... They have a heteromeric structure in that each subunit consists of the extracellular ligand-binding domain and a transmembrane ... The loops connecting the alpha helices form extracellular and intracellular domains. The binding-site for larger peptide ... The N terminus interacts with other cellular transcription factors in a ligand-independent manner; and, depending on these ...
"The XPB subunit of repair/transcription factor TFIIH directly interacts with SUG1, a subunit of the 26S proteasome and putative ... which interacts with the seven-membered alpha ring of 20S core particle and eastablishs an asymmetric interface between the 19S ... It also have subunits that can bind with nucleotides (e.g., ATPs) in order to facilitate the association between 19S and 20S ... These subunits can be categorized into two classes based on the ATP dependence of subunits, ATP-dependent subunits and ATP- ...
... core binding factor alpha 2 subunit MeSH D12.776.930.155.200.300 -- core binding factor alpha 3 subunit MeSH D12.776.930.316. ... nf-e2 transcription factor, p45 subunit MeSH D12.776.930.155.200.100 -- core binding factor alpha 1 subunit MeSH D12.776. ... CCAAT-binding factor MeSH D12.776.930.127.124.500 -- CCAAT-enhancer-binding protein-alpha MeSH D12.776.930.127.124.750 -- CCAAT ... alpha subunit MeSH D12.776.930.125.750.590 -- myod protein MeSH D12.776.930.125.750.595 -- myogenic regulatory factor 5 MeSH ...
"BRCA1 augments transcription by the NF-kappaB transcription factor by binding to the Rel domain of the p65/RelA subunit". The ... Karetsou Z, Kretsovali A, Murphy C, Tsolas O, Papamarcaki T (April 2002). "Prothymosin alpha interacts with the CREB-binding ... "Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) ... "DAF-16 recruits the CREB-binding protein coactivator complex to the insulin-like growth factor binding protein 1 promoter in ...
... caused by the alpha decay of actinium-227.[35] Perey then attempted to determine the proportion of beta decay to alpha decay in ... Gottschlich, Michele M. (2001). The Science and Practice of Nutrition Support: A Case-based Core Curriculum. Kendall Hunt. p. ... The first factor depends on the volume of the atom and thus the atomic radius, which increases going down the group; thus, the ... Solid phenyllithium forms monoclinic crystals can be described as consisting of dimeric Li2(C6H5)2 subunits. The lithium atoms ...
... core binding factor plays a key role in several development pathways and in human disease; has been sequenced ... Transcription Factors: 20597*Core Binding Factors: 223*Core Binding Factor alpha Subunits: 18*core binding factor alpha: 16 ... core binding factor alpha. Subscribe to New Research on core binding factor alpha ... 11/07/2000 - "Binding of CBFalpha/AML/PEBP2alpha (core binding factor alpha/acute myelogenous leukemia/polyoma enhancer binding ...
Core Binding Factor Alpha 1 Subunit / metabolism* * Epithelial-Mesenchymal Transition / drug effects ... Runx2 stimulated SNAI2 expression in a WNT- and transforming growth factor (TGF)β-dependent manner, and knockdown of SNAI2 ... Nyam-Osor Chimge 1 , Sanjeev K Baniwal, Gillian H Little, Yi-bu Chen, Michael Kahn, Debu Tripathy, Zea Borok, Baruch Frenkel ... 1 Department of Biochemistry & Molecular Biology, Keck School of Medicine of the University of Southern California, 2250 ...
Core Binding Factor Alpha 1 Subunit / genetics * Core Binding Factor Alpha 1 Subunit / metabolism ... Jin-fang Zhang 1 , Guo Li, Chu-yan Chan, Chun-ling Meng, Marie Chia-mi Lin, Yang-chao Chen, Ming-liang He, Ping-chung Leung, ... The osteogenic effect was inhibited by the introduction of noggin and DKK-1, which is classical inhibitor of BMP and Wnt/beta- ... 2010 Jan 15;314(1):70-4. doi: 10.1016/j.mce.2009.08.012. Epub 2009 Aug 22. ...
Core Binding Factor Alpha 1 Subunit * DNA Primers * Enhancer Elements, Genetic * Genes, Regulator ... 1999 Apr 1;73(1):114-25. Authors M H Lee 1 , A Javed, H J Kim, H I Shin, S Gutierrez, J Y Choi, V Rosen, J L Stein, A J van ... However, other factors induced by BMP-2 appear to be necessary for complete expression of the osteoblast phenotype. ... Transient upregulation of CBFA1 in response to bone morphogenetic protein-2 and transforming growth factor beta1 in C2C12 ...
The analysis leads to a better insight of proteins that bind to DNA, regulate DNA, and function in chromatin remodeling. The ... The study summarizes transcriptional regulation factors interacting and cooperating at promoter regions that regulate gene ... Core-binding factor alpha-subunit 1. Osteoblast-specific transcription factor. Cleidocranial dysplasia murine Cbfa1+/- ... Transcription factors bind to regulatory elements upstream of transcription start sites and interact with other factors to ...
... core-binding factor alpha subunit 1 (Cbfa1; 7.2-fold), osteocalcin (OC; 430-fold), and BSP (BSP; 5.7-fold) transcripts (Fig. 1 ... B and C) Newly formed vessels, identified by CD31+ endothelial cells, penetrated the outer matrix and reached the inner core in ... an inner hypertrophic core (B, D, and F) rich in GAG, Col II, and Col X, and an outer mineralized rim (B, H, J, and L) with a ... 1997) Fibroblast growth factor-2 supports ex vivo expansion and maintenance of osteogenic precursors from human bone marrow. ...
Core Binding Factor Alpha 1 Subunit / genetics * Core Binding Factor Alpha 1 Subunit / metabolism ... Chenghai Li 1 , Kristifor Sunderic 1 , Steven B Nicoll 1 , Sihong Wang 2 ... DOI: 10.1038/s41598-017-18541-1 Abstract Human mesenchymal stem cells (hMSCs) show promise for bone and cartilage regeneration ... 1 Department of Biomedical Engineering, City University of New York-City College, 160 Convent Avenue, New York, NY, 10031, USA. ...
Core-binding factor subunit alpha-1; Short=CBF-alpha-1;AltName: Full=Oncogene AML-3;AltName: Full=Osteoblast-specific ... the heterodimeric partner of a novel Drosophila runt-related DNA binding protein PEBP2 alpha. Virology. 1993 May;194(1):314-31 ... J:19082 Ogawa E, et al., PEBP2/PEA2 represents a family of transcription factors homologous to the products of the Drosophila ... J:49076 Xiao ZS, et al., Genomic structure and isoform expression of the mouse, rat and human Cbfa1/Osf2 transcription factor. ...
SL3/AKV core binding factor alpha A subunit. *SL3/AKV core-binding factor alpha A subunit ... CBF binds to the core site, 5-PYGPYGGT-3, of a number of enhancers and promoters, including murine leukemia virus, ... Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of ... alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. ...
... to runt-related transcription factor 2 (RUNX2) in the mDE6 cells. Several s ... Core Binding Factor Alpha 1 Subunit/genetics*/metabolism. *Epithelial Cells/metabolism*. *Proto-Oncogene Proteins c-akt/ ... and can induce the expression of runt-related transcription factor 2 (RUNX2) during the development of the tooth germ.The ... and can induce the expression of runt-related transcription factor 2 (RUNX2) during the development of the tooth germ. RUNX2 ...
... core-binding factor subunit alpha-1 (Cbfa1) and receptor activator of nuclear factor kappa-B ligand (RANKL), which is regulated ... Aliskiren is a drug of the second generation of renin inhibitors, and it binds to the binding site of renin essential for its ... Renin inhibitors bind to the active site of renin and inhibit its binding to angiotensinogen, which is the rate-determining ... Aliskiren binds to the binding site of renin essential for its activity thereby reducing plasma renin activity and suppressing ...
Core Binding Factor alpha Subunits * Transcriptional Activation * leucine-rich repeat proteins * Leukemia ...
SL3/AKV core binding factor alpha A subunit antibody. *SL3/AKV core-binding factor alpha A subunit antibody ... Polyomavirus enhancer binding protein 2 alpha A subunit antibody. *Polyomavirus enhancer-binding protein 2 alpha A subunit ... Core binding factor antibody. *Core binding factor runt domain alpha subunit 1 antibody ... CBF binds to the core site, 5-PYGPYGGT-3, of a number of enhancers and promoters, including murine leukemia virus, ...
Core Binding Factor Alpha 1 Subunit/genetics. *DNA Primers. *Flow Cytometry. *Gene Expression Profiling ... Role of Slug transcription factor in human mesenchymal stem cells. Torreggiani E, Lisignoli G, Manferdini C, Lambertini E, ... Role of Slug transcription factor in human mesenchymal stem cells. Torreggiani E, Lisignoli G, Manferdini C, Lambertini E, ...
... bone differentiation depends on pRb coactivation of the RUNT-RELATED TRANSCRIPTION FACTOR2/CORE-BINDING FACTOR SUBUNIT ALPHA-1 ... pRB is known to promote cell cycle exit by binding to members of the E2F family of transcription factors, thus interfering with ... pRB acts as a transcriptional regulator primarily via a mechanism that consists of binding to the main transcription factor ... a transcription factor involved in cell differentiation. We show that both RBR and the cytokinin-dependent transcription factor ...
Core Binding Factor Alpha 1 Subunit Genes Co-Repressor Proteins Gene expression ... an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. Runx2, ... an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. Runx2, ... an osteoblast-related transcription factor, and suppresses Runx2 transcriptional activity in a dose-dependent manner. Runx2, ...
The transforming growth factor beta (TGF-beta) family member bone morphogenetic protein-3B (BMP-3B/GDF10) is regarded as a ... The ectopic expression of Runx2, but not DNA binding mutant Runx2, in normal lung fibroblast cells and lung cancer cells ... The Runt-related transcription factor Runx2 is essential for bone development but is also implicated in progression of several ... Core Binding Factor Alpha 1 Subunit; Bone Morphogenetic Protein 3; Lung Neoplasms ...
CBF-alpha-2; core binding factor alpha 2; Core-binding factor subunit alpha-2; core-binding factor, runt domain, alpha subunit ... PEA2-alpha B; PEBP2-alpha B; Polyomavirus enhancer-binding protein 2 alpha B subunit; RUN1; runt domain, alpha subunit 2; Runt- ... SL3/AKV core-binding factor alpha B subunit; transcription factor Gene Aliases: AML1; AML1-EVI-1; AMLCR1; CBF-alpha-2; ... DNA binding transcription factor activity, sequence-specific DNA binding calcium ion binding transcription factor binding ...
Runt-related transcription factor 2 (RUNX2) also known as core-binding factor subunit alpha-1 (CBF-alpha-1) is a protein that ... The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Transcript variants ... This protein is a member of the RUNX family of transcription factors and has a Runt DNA-binding domain. It is essential for ... The phosphorylation state of Runx2 also mediates its DNA-binding activity. Interestingly, the Runx2 DNA-binding activity is ...
core-binding factor, runt domain, alpha subunit 2; translocated to, 3 Calculated molecular weight: ... Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP(CBFA2T3 antibody) at dilution of 1:1000 ... Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP(CBFA2T3 antibody) at dilution of 1:1000 ... Jurkat cells were subjected to SDS PAGE followed by western blot with 17190-1-AP(CBFA2T3 antibody) at dilution of 1:1000 ...
Core Binding Factor Alpha 1 Subunit / metabolism. Eyelid Neoplasms / metabolism. Osteoblasts / metabolism ... Chemical-registry-number] 0 / Antigens, Differentiation; 0 / Core Binding Factor Alpha 1 Subunit ... In this review, we discuss the risk factors, pathology, molecular biology, clinical features, and management of eyelid basal ... 1. Jankovic I, Kovacevic P, Visnjic M, Jankovic D, Binic I, Jankovic A: Does incomplete excision of basal cell carcinoma of the ...
... core-binding factor (CBF), alpha-B subunit, etc.) binds to the core site, 5-pygpyggt-3, of a number of enhancers and ... The protein is a heterodimer of alpha- and beta-subunits. The alpha-subunit binds DNA as a monomer, and appears to have a role ... highly similar to the Drosophila melanogaster segmentation gene runt and to the mouse transcription factor PEBP2 alpha subunit ... The region of shared similarity, known as the Runt domain, is responsible for DNA-binding and protein-protein interaction. In ...
Runt-related transcription factor 1(RUNX1) plays a vital role in hematopoiesis via Endothelial-to-Hematop … ... Core Binding Factor Alpha 2 Subunit / genetics Actions. * Search in PubMed * Search in MeSH ... and Renal Fibrosis Through the PI3K Subunit p110δ Tong Zhou 1 , Maocai Luo 2 , Wei Cai 3 , Siyuan Zhou 2 , Danying Feng 2 , ... Tong Zhou 1 , Maocai Luo 2 , Wei Cai 3 , Siyuan Zhou 2 , Danying Feng 2 , Chundi Xu 4 , Hongyan Wang 5 ...
The Runx2 transcription factor is a key regulator of osteoblast differentiation. In response to 1alpha,25 dihydroxy vitamin D3 ... Absorptiometry, Photon; Animals; Core Binding Factor Alpha 1 Subunit; Crystallization; Mice; Protein Binding; Protein Structure ... The Runx2 transcription factor is a key regulator of osteoblast differentiation. In response to 1alpha,25 dihydroxy vitamin D3 ... Crystallization and preliminary X-ray analysis of a domain in the Runx2 transcription factor that interacts with the 1alpha,25 ...
Point mutants defective for binding to WW domain or SMAD proteins or the nuclear matrix retain this growth regulatory ability. ... transforming growth factor-beta/BMP-SMAD, SRC/YES-YAP, and GROUCHO/TLE). Runx2 levels oscillate during the osteoblast cell ... However, mutants defective for DNA binding or C-terminal gene repression/activation functions do not block proliferation. ... Runt-related transcription factor 2 (Runx2) controls lineage commitment, proliferation, and anabolic functions of osteoblasts ...
  • ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. (wikipedia.org)
  • The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. (wikipedia.org)
  • The F1 fraction derives its name from the term "Fraction 1" and Fo (written as a subscript letter "o", not "zero") derives its name from being the binding fraction for oligomycin, a type of naturally-derived antibiotic that is able to inhibit the Fo unit of ATP synthase. (wikipedia.org)
  • Specifically, the γ subunit includes four particular Cystathionine beta synthase (CBS) domains giving AMPK its ability to sensitively detect shifts in the AMP:ATP ratio. (wikipedia.org)
  • Interaction of the C-terminal domain of p43 and the alpha subunit of ATP synthase. (wikipedia.org)
  • Non-ligand bound Ahr is retained in the cytoplasm as an inactive protein complex consisting of a dimer of Hsp90, prostaglandin E synthase 3 (PTGES3, p23) and a single molecule of the immunophilin-like AH receptor-interacting protein, also known as hepatitis B virus X-associated protein 2 (XAP2), AhR interacting protein (AIP), and AhR-activated 9 (ARA9). (wikipedia.org)
  • Putative substrates of parkin include synphilin-1, CDC-rel1, cyclin E, p38 tRNA synthase, Pael-R, synaptotagmin XI, sp22 and parkin itself (see also ubiquitin ligase). (wikipedia.org)
  • These effects were abolished with cotreatment by ACE inhibitors or angiotensin type 1 receptor blockers (ARBs). (hindawi.com)
  • CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, LCK, IL-3 and GM-CSF promoters. (acris-antibodies.com)
  • Kikuchi Y, Yasue T, Miyake K, Kimoto M, Takatsu K. CD38 ligation induces tyrosine phosphorylation of Bruton tyrosine kinase and enhanced expression of interleukin 5-receptor alpha chain: synergistic effects with interleukin 5. (labome.org)
  • These data indicate that CD38 ligation increases IL-5 receptor alpha expression and synergizes with IL-5 to enhance Blimp1 expression and IgM synthesis. (labome.org)
  • Interferon-alpha and interleukin-12 are induced differentially by Toll-like receptor 7 ligands in human blood dendritic cell subsets. (labome.org)
  • Adams B, Furneaux H, White B. The micro-ribonucleic acid (miRNA) miR-206 targets the human estrogen receptor-alpha (ERalpha) and represses ERalpha messenger RNA and protein expression in breast cancer cell lines. (labome.org)
  • A transmembrane protein belonging to the tumor necrosis factor superfamily that specifically binds RECEPTOR ACTIVATOR OF NUCLEAR FACTOR-KAPPA B and OSTEOPROTEGERIN. (umassmed.edu)
  • Distribution of mRNAs encoding classical progestin receptor, progesterone membrane components 1 and 2, serpine mRNA binding protein 1, and progestin and ADIPOQ receptor family members 7 and 8 in rat forebrain. (umassmed.edu)
  • A discoidin domain receptor 1 knock-out mouse as a novel model for osteoarthritis of the temporomandibular joint. (ox.ac.uk)
  • Discoidin domain receptor 1 (DDR-1)-deficient mice exhibited a high incidence of osteoarthritis (OA) in the temporomandibular joint (TMJ) as early as 9 weeks of age. (ox.ac.uk)
  • Activation of Sirt-1 by resveratrol in MSCs increased its binding to PPAR-γ and repressed PPAR-γ activity by involving its cofactor NCoR (nuclear receptor co-repressor). (nih.gov)
  • These data suggest that PS-1 may associate with a PDZ-like domain-containing protein in vivo and thus may participate in receptor or channel clustering and intracellular signaling events in the brain. (labome.org)
  • In addition to participation in proteasome functions, this subunit may participate in transcriptional regulation since it has been shown to interact with the thyroid hormone receptor and retinoid X receptor-alpha. (wikipedia.org)
  • When such chemical signals bind to a receptor, they cause some form of cellular/tissue response, e.g. a change in the electrical activity of a cell. (wikipedia.org)
  • A molecule that binds to a receptor is called a ligand, and can be a protein or peptide (short protein), or another small molecule such as a neurotransmitter, hormone, pharmaceutical drug, toxin, or parts of the outside of a virus or microbe. (wikipedia.org)
  • While numerous receptors are found in most cells, each receptor will only bind with ligands of a particular structure, much like how locks will only accept specifically shaped keys. (wikipedia.org)
  • When a ligand binds to its corresponding receptor, it activates or inhibits the receptor's associated biochemical pathway. (wikipedia.org)
  • Type 3: Kinase-linked and related receptors (see "Receptor tyrosine kinase", and "Enzyme-linked receptor") - They are composed of an extracellular domain containing the ligand binding site and an intracellular domain, often with enzymatic-function, linked by a single transmembrane alpha helix. (wikipedia.org)
  • The core region has two zinc fingers that are responsible for recognizing the DNA sequences specific to this receptor. (wikipedia.org)
  • and, depending on these interactions, it can modify the binding/activity of the receptor. (wikipedia.org)
  • The binding of a ligand to the receptor on the ion channel protein causes a conformational change which allows the passing of certain ions. (wikipedia.org)
  • Insulin receptor substrate 1 (IRS-1). (wikipedia.org)
  • SMARCE1 has been shown to interact with Estrogen receptor alpha, SMARCB1 and SMARCA4. (wikipedia.org)
  • Another commensal species, B. thetaiotaomicron, attenuates pro-inflammatory cytokine expression by promoting nuclear export of NF-κB subunit RelA, through a peroxisome proliferator activated receptor γ (PPAR-γ)-dependent pathway. (wikipedia.org)
  • The response is terminated upon GTP hydrolysis by the alpha subunit (InterPro: IPR001019), which can then re-bind the beta-gamma dimer (InterPro: IPR001632 InterPro: IPR001770) and the receptor. (wikipedia.org)
  • HIF1 is a heterodimeric basic helix-loop-helix structure that is composed of HIF1A, the alpha subunit (this protein), and the aryl hydrocarbon receptor nuclear translocator (Arnt), the beta subunit. (wikipedia.org)
  • When the four subunits of the tetramer come together, this second membranous domain forms the ion-permeable pore of the receptor. (wikipedia.org)
  • MTA2 deacetylates the estrogen receptor alpha and p53 and inhibits their transactivation functions. (wikipedia.org)
  • When a G protein-coupled receptor (GPCR) is activated, Gα dissociates from Gβγ, allowing both subunits to perform their respective downstream signaling effects. (wikipedia.org)
  • The aryl hydrocarbon receptor is a ligand-activated transcription factor involved in the regulation of biological responses to planar aromatic (aryl) hydrocarbons. (wikipedia.org)
  • The dimer of Hsp90, along with PTGES3 (p23), has a multifunctional role in the protection of the receptor from proteolysis, constraining the receptor in a conformation receptive to ligand binding and preventing the premature binding of ARNT. (wikipedia.org)
  • http://www.springerimages.com/Images/RSS/1-10.1007_s00216-009-2785-x-0 Xu C, Rosen BP (1997). (wikipedia.org)
  • MBS2702491 is a ready-to-use microwell, strip plate Double-antibody Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Hypoxia Inducible Factor 1 Alpha (HIF1a) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • This two-subunit enzyme produces resistance to arsenite and antimonite. (wikipedia.org)
  • These proteolytic active sites located in the inner side of a chamber formed by 4 stacked rings of 20S subunits, preventing random protein-enzyme encounter and uncontrolled protein degradation. (wikipedia.org)
  • ATPases (or ATP synthases) are membrane-bound enzyme complexes/ion transporters that combine ATP synthesis and/or hydrolysis with the transport of protons across a membrane. (wikipedia.org)
  • The Polδ enzyme is responsible for synthesizing the lagging strand of DNA, and has also been implicated in some activities at the leading strand (Figure 1). (wikipedia.org)
  • The hydroxylated proline residue of HIF1A is then recognized and buried in the hydrophobic core of von Hippel-Lindau tumor suppressor protein (VHL), which itself is part of a ubiquitin ligase enzyme. (wikipedia.org)
  • Both binding of substrates and catalysis depend on the three-dimensional structure of the enzyme which arises as a consequence of the level of protein folding. (wikipedia.org)
  • Additional factors like temperature, pH and metal ions influence the non-covalent interactions between enzyme structure. (wikipedia.org)
  • The enzyme is categorised as an endoglucanase, which internally cleaves β-1,4 -glycosydic bonds in cellulose chains facilitating further degradation of the polymer. (wikipedia.org)
  • RING1 forms the binding site for E2 Ub-conjugating enzyme while RING2 contains the catalytic cysteine residue (Cys431) that cleaves Ub off E2 and transiently binds it to E3 via a thioester bond. (wikipedia.org)
  • Choi J, Lee K, Ingvarsdottir K, Bonasio R, Saraf A, Florens L, Washburn MP, Tadros S, Green MR, Busino L. Loss of KLHL6 promotes diffuse large B-cell lymphoma growth and survival by stabilizing the mRNA decay factor roquin2. (umassmed.edu)
  • The DEAD-box helicase Dbp5/Rat8 is an essential mRNA export factor that has also been implicated in other steps of mRNA. (dartmouth.edu)
  • The 40S subunit contains the decoding center which monitors the complementarity of tRNA and mRNA in protein translation. (wikipedia.org)
  • The mRNA binds in the cleft between the head and the body, and there are three binding sites for tRNA, the A-site, P-site and E-site (see article on protein translation for details). (wikipedia.org)
  • The core protein mRNA levels of proteoglycans were not affected, thus the decrease in GAG chains was as a result of some other factor, which in this case turned out to be a decrease in expression of sulfate transferase enzymes, which play a key role in GAG biosynthesis. (wikipedia.org)
  • Mir-132 microRNA has been reported to interact with Sirtuin 1 mRNA, so as to reduce protein expression. (wikipedia.org)
  • Regulated by MITF) HMGB1 High mobility group box binds DNA ILF2 Homo sapiens interleukin enhancer binding factor 2, 45kDa (ILF2), mRNA IER2 formerly ETR101 Immediate Early Protein? (wikipedia.org)
  • However, activation of CBP by HIPK2 is not mediated by this phosphorylation but rather by counteracting the repressive action of the cell cycle regulatory domain 1 (CRD1) of CBP, located between amino acids 977 and 1076. (wikipedia.org)
  • GluA1 has four known phosphorylation sites at serine 818 (S818), S831, threonine 840, and S845 (other subunits have similar phosphorylation sites, but GluR1 has been the most extensively studied). (wikipedia.org)
  • Although structural changes following phosphorylation are uncertain, crystallisation of parkin revealed a cationic pocket in RING0 formed by lysine and arginine residues Lys161, Arg163 and Lys211 that forms a putative phosphate binding site. (wikipedia.org)
  • Thus, Txk is expressed on Th1/Th0 cells with the IFN-gamma production and acts as a Th1 cell-specific transcription factor. (labome.org)
  • Sequential polarization and imprinting of type 1 T helper lymphocytes by interferon-gamma and interleukin-12. (nih.gov)
  • It is puzzling that interferon-gamma induces the Th1 transcription factor T-bet, whereas interleukin-12 mediates Th1 cell lineage differentiation. (nih.gov)
  • AMPK is regulated allosterically mostly by competitive binding on its gamma subunit between ATP (which allows phosphatase access to T172) and AMP or ADP (each of which blocks access to phosphatases). (wikipedia.org)
  • The conformation of the epsilon subunit is determined by the direction of rotation of the gamma subunit, and possibly by the presence of ADP. (wikipedia.org)
  • c-Jun N-terminal kinase (JNK)-interacting protein-1b/islet-brain-1 scaffolds Alzheimer's amyloid precursor protein with JNK. (labome.org)
  • Analysis of the signal transduction mechanisms underlying the upregulating effect of RA on collagenase-3 expression demonstrated that this factor acts through a signaling pathway involving p38 mitogen-activated protein kinase. (nih.gov)
  • Ceramide kinase, a lipid kinase that phosphorylates ceramides to ceramide-1-phosphate. (wikipedia.org)
  • The core domains of the PAK family include a kinase domain in the C-terminal region, a p21-binding domain (PBD), and an auto-inhibitory domain (AID) in group I PAKs. (wikipedia.org)
  • Group I PAKs exist in an inactive, closed homodimer conformation wherein AID of one molecule binds to the kinase domain of another molecule, and activated in both GTPase-dependent and -independent manners. (wikipedia.org)
  • This study determined whether activation of Sirt-1 (a NAD(+)-dependent histone deacetylase) by the phytoestrogen resveratrol affects osteogenic differentiation. (nih.gov)
  • The conserved region contains four alpha helices and three loops arranged as in histone H3. (wikipedia.org)
  • Such remodeling is principally carried out by 1) covalent histone modifications by specific enzymes, e.g., histone acetyltransferases (HATs), deacetylases, methyltransferases, and kinases, and 2) ATP-dependent chromatin remodeling complexes which either move, eject or restructure nucleosomes. (wikipedia.org)
  • This is because over 90% of mammalian promoters do not contain a BRE (B recognition element) or TATA box sequence which are required for TFIIB to bind. (wikipedia.org)
  • However, although it would seem that PHD2 downregulates HIF-1α and thus also tumorigenesis, there have been suggestions of paradoxical roles of PHD2 in tumor proliferation. (wikipedia.org)
  • For example, methylselenocysteine (MSC) inhibition of HIF-1α led to tumor growth inhibition in renal cell carcinoma in a PHD-dependent manner. (wikipedia.org)
  • MSX1 transcripts are not only found in thyrotrope-derived TSH cells, but also in the TtT97 thyrotropic tumor, which is a well differentiated hyperplastic tissue that produces both TSHß- and a-subunits and is responsive to thyroid hormone. (wikipedia.org)
  • The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. (wikipedia.org)