Coated Pits, Cell-Membrane
Coated Vesicles
Clathrin
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.
Synaptic Vesicles
Membrane-bound compartments which contain transmitter molecules. Synaptic vesicles are concentrated at presynaptic terminals. They actively sequester transmitter molecules from the cytoplasm. In at least some synapses, transmitter release occurs by fusion of these vesicles with the presynaptic membrane, followed by exocytosis of their contents.
Endosomes
Organoids
Microscopy, Electron
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
Endocytosis
Transport Vesicles
Golgi Apparatus
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Coatomer Protein
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
Cytoplasmic Vesicles
COP-Coated Vesicles
TRANSPORT VESICLES formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of COP (coat protein complex) proteins, either COPI or COPII. COPI coated vesicles transport backwards from the cisternae of the GOLGI APPARATUS to the rough endoplasmic reticulum (ENDOPLASMIC RETICULUM, ROUGH), while COPII coated vesicles transport forward from the rough endoplasmic reticulum to the Golgi apparatus.
Seminal Vesicles
Adaptor Proteins, Vesicular Transport
A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. The majority of adaptor proteins exist as multi-subunit complexes, however monomeric varieties have also been found.
Cell Fractionation
Clathrin-Coated Vesicles
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.
Secretory Vesicles
Cattle
Adaptor Protein Complex alpha Subunits
Cell Membrane
Brain
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Intracellular Membranes
Monomeric Clathrin Assembly Proteins
Receptor, IGF Type 2
Adaptor Protein Complex gamma Subunits
Coat Protein Complex I
Adaptor Protein Complex 2
Taurodeoxycholic Acid
Exocytosis
Biological Transport
Vesicular Transport Proteins
A broad category of proteins involved in the formation, transport and dissolution of TRANSPORT VESICLES. They play a role in the intracellular transport of molecules contained within membrane vesicles. Vesicular transport proteins are distinguished from MEMBRANE TRANSPORT PROTEINS, which move molecules across membranes, by the mode in which the molecules are transported.
Dynamins
Liposomes
Membrane Proteins
HSC70 Heat-Shock Proteins
Organelles
Transferrin
Lysosomes
A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Microscopy, Immunoelectron
Adaptor Protein Complex beta Subunits
Vacuoles
Adaptor Protein Complex mu Subunits
ADP-Ribosylation Factor 1
Synaptic Membranes
Brefeldin A
Carrier Proteins
ADP-Ribosylation Factors
Endoplasmic Reticulum
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
Freeze Etching
A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces.
Pinocytosis
alpha-L-Fucosidase
Electrophoresis, Polyacrylamide Gel
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Brain Chemistry
Proton-Translocating ATPases
Amino Acid Sequence
Protein Binding
Hydrogen-Ion Concentration
Proteins
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
Auxilins
Freeze Fracturing
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
Adenosine Triphosphate
Receptors, Transferrin
Membrane Lipids
Lipids, predominantly phospholipids, cholesterol and small amounts of glycolipids found in membranes including cellular and intracellular membranes. These lipids may be arranged in bilayers in the membranes with integral proteins between the layers and peripheral proteins attached to the outside. Membrane lipids are required for active transport, several enzymatic activities and membrane formation.
Adenosine Triphosphatases
Adaptor Protein Complex sigma Subunits
Multiprotein Complexes
Macromolecular Substances
Liver
Cytoplasm
Cell Compartmentation
Membranes
Peptide Hydrolases
Subcellular Fractions
Components of a cell produced by various separation techniques which, though they disrupt the delicate anatomy of a cell, preserve the structure and physiology of its functioning constituents for biochemical and ultrastructural analysis. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p163)
Protein Transport
alpha-Mannosidase
An enzyme that catalyzes the HYDROLYSIS of terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. The enzyme plays a role in the processing of newly formed N-glycans and in degradation of mature GLYCOPROTEINS. There are multiple isoforms of alpha-mannosidase, each having its own specific cellular location and pH optimum. Defects in the lysosomal form of the enzyme results in a buildup of mannoside intermediate metabolites and the disease ALPHA-MANNOSIDOSIS.
Centrifugation, Density Gradient
Cytosol
Filipin
Adaptor Protein Complex 1
GTP-Binding Proteins
Regulatory proteins that act as molecular switches. They control a wide range of biological processes including: receptor signaling, intracellular signal transduction pathways, and protein synthesis. Their activity is regulated by factors that control their ability to bind to and hydrolyze GTP to GDP. EC 3.6.1.-.
Horseradish Peroxidase
Guanosine Triphosphate
Ubiquitin-Protein Ligases
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
Immunologic Capping
An energy dependent process following the crosslinking of B CELL ANTIGEN RECEPTORS by multivalent ligands (bivalent anti-antibodies, LECTINS or ANTIGENS), on the B-cell surface. The crosslinked ligand-antigen receptor complexes collect in patches which flow to and aggregate at one pole of the cell to form a large mass - the cap. The caps may then be endocytosed or shed into the environment.
Nerve Endings
Branch-like terminations of NERVE FIBERS, sensory or motor NEURONS. Endings of sensory neurons are the beginnings of afferent pathway to the CENTRAL NERVOUS SYSTEM. Endings of motor neurons are the terminals of axons at the muscle cells. Nerve endings which release neurotransmitters are called PRESYNAPTIC TERMINALS.
Histocytochemistry
Membrane Fusion
Fluorescent Antibody Technique
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Electrophoresis, Agar Gel
Phosphatidylcholines
Models, Biological
Arabidopsis Proteins
Cells, Cultured
Mutation
Models, Structural
Rabbits
Calcium
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Phospholipids
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Arabidopsis
Lipid Bilayers
Asialoglycoprotein Receptor
Calcium-Binding Proteins
HeLa Cells
Immunologic Techniques
Chromatography, Gel
Ultracentrifugation
Chorionic Villi
Receptors, Cell Surface
Cell surface proteins that bind signalling molecules external to the cell with high affinity and convert this extracellular event into one or more intracellular signals that alter the behavior of the target cell (From Alberts, Molecular Biology of the Cell, 2nd ed, pp693-5). Cell surface receptors, unlike enzymes, do not chemically alter their ligands.
Acid Phosphatase
Cricetinae
beta-N-Acetylhexosaminidases
A hexosaminidase specific for non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides. It acts on GLUCOSIDES; GALACTOSIDES; and several OLIGOSACCHARIDES. Two specific mammalian isoenzymes of beta-N-acetylhexoaminidase are referred to as HEXOSAMINIDASE A and HEXOSAMINIDASE B. Deficiency of the type A isoenzyme causes TAY-SACHS DISEASE, while deficiency of both A and B isozymes causes SANDHOFF DISEASE. The enzyme has also been used as a tumor marker to distinguish between malignant and benign disease.
Ferritins
Iron-containing proteins that are widely distributed in animals, plants, and microorganisms. Their major function is to store IRON in a nontoxic bioavailable form. Each ferritin molecule consists of ferric iron in a hollow protein shell (APOFERRITINS) made of 24 subunits of various sequences depending on the species and tissue types.
Biological Transport, Active
Recombinant Fusion Proteins
Rats, Inbred Strains
SNARE Proteins
A superfamily of small proteins which are involved in the MEMBRANE FUSION events, intracellular protein trafficking and secretory processes. They share a homologous SNARE motif. The SNARE proteins are divided into subfamilies: QA-SNARES; QB-SNARES; QC-SNARES; and R-SNARES. The formation of a SNARE complex (composed of one each of the four different types SNARE domains (Qa, Qb, Qc, and R)) mediates MEMBRANE FUSION. Following membrane fusion SNARE complexes are dissociated by the NSFs (N-ETHYLMALEIMIDE-SENSITIVE FACTORS), in conjunction with SOLUBLE NSF ATTACHMENT PROTEIN, i.e., SNAPs (no relation to SNAP 25.)
Swine
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Peptides
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Binding Sites
Acetylcholinesterase
Chromatography
Techniques used to separate mixtures of substances based on differences in the relative affinities of the substances for mobile and stationary phases. A mobile phase (fluid or gas) passes through a column containing a stationary phase of porous solid or liquid coated on a solid support. Usage is both analytical for small amounts and preparative for bulk amounts.
Synaptotagmins
HSP70 Heat-Shock Proteins
Presynaptic Terminals
The distal terminations of axons which are specialized for the release of neurotransmitters. Also included are varicosities along the course of axons which have similar specializations and also release transmitters. Presynaptic terminals in both the central and peripheral nervous systems are included.
Hexosaminidases
Saccharomyces cerevisiae
Protein Conformation
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Hypocotyl
The region of the stem beneath the stalks of the seed leaves (cotyledons) and directly above the young root of the embryo plant. It grows rapidly in seedlings showing epigeal germination and lifts the cotyledons above the soil surface. In this region (the transition zone) the arrangement of vascular bundles in the root changes to that of the stem. (From Concise Dictionary of Biology, 1990)
Postural Balance
Cullin Proteins
Chromatography, Affinity
Membranes, Artificial
Protein Kinases
Adrenal Cortex
The outer layer of the adrenal gland. It is derived from MESODERM and comprised of three zones (outer ZONA GLOMERULOSA, middle ZONA FASCICULATA, and inner ZONA RETICULARIS) with each producing various steroids preferentially, such as ALDOSTERONE; HYDROCORTISONE; DEHYDROEPIANDROSTERONE; and ANDROSTENEDIONE. Adrenal cortex function is regulated by pituitary ADRENOCORTICOTROPIN.
Glycoproteins
rab GTP-Binding Proteins
Synapsins
A family of synaptic vesicle-associated proteins involved in the short-term regulation of NEUROTRANSMITTER release. Synapsin I, the predominant member of this family, links SYNAPTIC VESICLES to ACTIN FILAMENTS in the presynaptic nerve terminal. These interactions are modulated by the reversible PHOSPHORYLATION of synapsin I through various signal transduction pathways. The protein is also a substrate for cAMP- and CALCIUM-CALMODULIN-DEPENDENT PROTEIN KINASES. It is believed that these functional properties are also shared by synapsin II.
Bungarotoxins
Neurotoxic proteins from the venom of the banded or Formosan krait (Bungarus multicinctus, an elapid snake). alpha-Bungarotoxin blocks nicotinic acetylcholine receptors and has been used to isolate and study them; beta- and gamma-bungarotoxins act presynaptically causing acetylcholine release and depletion. Both alpha and beta forms have been characterized, the alpha being similar to the large, long or Type II neurotoxins from other elapid venoms.
Antibodies
Qa-SNARE Proteins
Placenta
A highly vascularized mammalian fetal-maternal organ and major site of transport of oxygen, nutrients, and fetal waste products. It includes a fetal portion (CHORIONIC VILLI) derived from TROPHOBLASTS and a maternal portion (DECIDUA) derived from the uterine ENDOMETRIUM. The placenta produces an array of steroid, protein and peptide hormones (PLACENTAL HORMONES).
Cytoskeleton
Plant Proteins
Immunohistochemistry
Trypsin
Microscopy, Fluorescence
Colloids
Two-phase systems in which one is uniformly dispersed in another as particles small enough so they cannot be filtered or will not settle out. The dispersing or continuous phase or medium envelops the particles of the discontinuous phase. All three states of matter can form colloids among each other.
Blotting, Western
Receptors, Cholinergic
Cell surface proteins that bind acetylcholine with high affinity and trigger intracellular changes influencing the behavior of cells. Cholinergic receptors are divided into two major classes, muscarinic and nicotinic, based originally on their affinity for nicotine and muscarine. Each group is further subdivided based on pharmacology, location, mode of action, and/or molecular biology.
Fibroblasts
Chick Embryo
Valinomycin
A cyclododecadepsipeptide ionophore antibiotic produced by Streptomyces fulvissimus and related to the enniatins. It is composed of 3 moles each of L-valine, D-alpha-hydroxyisovaleric acid, D-valine, and L-lactic acid linked alternately to form a 36-membered ring. (From Merck Index, 11th ed) Valinomycin is a potassium selective ionophore and is commonly used as a tool in biochemical studies.
Microscopy, Electron, Transmission
Calmodulin
A heat-stable, low-molecular-weight activator protein found mainly in the brain and heart. The binding of calcium ions to this protein allows this protein to bind to cyclic nucleotide phosphodiesterases and to adenyl cyclase with subsequent activation. Thereby this protein modulates cyclic AMP and cyclic GMP levels.
Two-Hybrid System Techniques
Screening techniques first developed in yeast to identify genes encoding interacting proteins. Variations are used to evaluate interplay between proteins and other molecules. Two-hybrid techniques refer to analysis for protein-protein interactions, one-hybrid for DNA-protein interactions, three-hybrid interactions for RNA-protein interactions or ligand-based interactions. Reverse n-hybrid techniques refer to analysis for mutations or other small molecules that dissociate known interactions.
Cryptogenic Organizing Pneumonia
An interstitial lung disease of unknown etiology, occurring between 21-80 years of age. It is characterized by a dramatic onset of a "pneumonia-like" illness with cough, fever, malaise, fatigue, and weight loss. Pathological features include prominent interstitial inflammation without collagen fibrosis, diffuse fibroblastic foci, and no microscopic honeycomb change. There is excessive proliferation of granulation tissue within small airways and alveolar ducts.
Fluorescent Dyes
Exosomes
Quaternary Ammonium Compounds
Chromaffin Cells
Cells that store epinephrine secretory vesicles. During times of stress, the nervous system signals the vesicles to secrete their hormonal content. Their name derives from their ability to stain a brownish color with chromic salts. Characteristically, they are located in the adrenal medulla and paraganglia (PARAGANGLIA, CHROMAFFIN) of the sympathetic nervous system.
Phosphorylation
Neurotransmitter Agents
Chickens
Synaptotagmin I
Synaptic Transmission
The communication from a NEURON to a target (neuron, muscle, or secretory cell) across a SYNAPSE. In chemical synaptic transmission, the presynaptic neuron releases a NEUROTRANSMITTER that diffuses across the synaptic cleft and binds to specific synaptic receptors, activating them. The activated receptors modulate specific ion channels and/or second-messenger systems in the postsynaptic cell. In electrical synaptic transmission, electrical signals are communicated as an ionic current flow across ELECTRICAL SYNAPSES.
Temperature
Morphogenesis
Phosphatidylserines
Phytochrome A
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Microscopy, Confocal
Synapses
Specialized junctions at which a neuron communicates with a target cell. At classical synapses, a neuron's presynaptic terminal releases a chemical transmitter stored in synaptic vesicles which diffuses across a narrow synaptic cleft and activates receptors on the postsynaptic membrane of the target cell. The target may be a dendrite, cell body, or axon of another neuron, or a specialized region of a muscle or secretory cell. Neurons may also communicate via direct electrical coupling with ELECTRICAL SYNAPSES. Several other non-synaptic chemical or electric signal transmitting processes occur via extracellular mediated interactions.
Tubulin
A microtubule subunit protein found in large quantities in mammalian brain. It has also been isolated from SPERM FLAGELLUM; CILIA; and other sources. Structurally, the protein is a dimer with a molecular weight of approximately 120,000 and a sedimentation coefficient of 5.8S. It binds to COLCHICINE; VINCRISTINE; and VINBLASTINE.
Sequence Homology, Amino Acid
Basic-Leucine Zipper Transcription Factors
Osmotic Pressure
Gene Expression Regulation, Plant
Phosphatidylglycerols
Clathrin and two components of the COPII complex, Sec23p and Sec24p, could be involved in endocytosis of the Saccharomyces cerevisiae maltose transporter. (1/342)
The Saccharomyces cerevisiae maltose transporter is a 12-transmembrane segment protein that under certain physiological conditions is degraded in the vacuole after internalization by endocytosis. Previous studies showed that endocytosis of this protein is dependent on the actin network, is independent of microtubules, and requires the binding of ubiquitin. In this work, we attempted to determine which coat proteins are involved in this endocytosis. Using mutants defective in the heavy chain of clathrin and in several subunits of the COPI and the COPII complexes, we found that clathrin, as well as two cytosolic subunits of COPII, Sec23p and Sec24p, could be involved in internalization of the yeast maltose transporter. The results also indicate that endocytosis of the maltose transporter and of the alpha-factor receptor could have different requirements. (+info)LST1 is a SEC24 homologue used for selective export of the plasma membrane ATPase from the endoplasmic reticulum. (2/342)
In Saccharomyces cerevisiae, vesicles that carry proteins from the ER to the Golgi compartment are encapsulated by COPII coat proteins. We identified mutations in ten genes, designated LST (lethal with sec-thirteen), that were lethal in combination with the COPII mutation sec13-1. LST1 showed synthetic-lethal interactions with the complete set of COPII genes, indicating that LST1 encodes a new COPII function. LST1 codes for a protein similar in sequence to the COPII subunit Sec24p. Like Sec24p, Lst1p is a peripheral ER membrane protein that binds to the COPII subunit Sec23p. Chromosomal deletion of LST1 is not lethal, but inhibits transport of the plasma membrane proton-ATPase (Pma1p) to the cell surface, causing poor growth on media of low pH. Localization by both immunofluorescence microscopy and cell fractionation shows that the export of Pma1p from the ER is impaired in lst1Delta mutants. Transport of other proteins from the ER was not affected by lst1Delta, nor was Pma1p transport found to be particularly sensitive to other COPII defects. Together, these findings suggest that a specialized form of the COPII coat subunit, with Lst1p in place of Sec24p, is used for the efficient packaging of Pma1p into vesicles derived from the ER. (+info)Identification of the putative mammalian orthologue of Sec31P, a component of the COPII coat. (3/342)
The regulation of intracellular vesicular trafficking is mediated by specific families of proteins that are involved in vesicular budding, translocation, and fusion with target membranes. We purified a vesicle-associated protein from hepatic microsomes using sequential column chromatography and partially sequenced it. Oliogonucleotides based on these sequences were used to clone the protein from a rat liver cDNA library. The clone encoded a novel protein with a predicted mass of 137 kDa (p137). The protein had an N terminus WD repeat motif with significant homology to Sec31p, a member of the yeast COPII coat that complexes with Sec13p. We found that p137 interacted with mammalian Sec13p using several approaches: co-elution through sequential column chromatography, co-immunoprecipitation from intact cells, and yeast two-hybrid analysis. Morphologically, the p137 protein was localized to small punctate structures in the cytoplasm of multiple cultured cell lines. When Sec13p was transfected into these cells, it demonstrated considerable overlap with p137. This overlap was maintained through several pharmacological manipulations. The p137 compartment also demonstrated partial overlap with ts045-VSVG protein when infected cells were incubated at 15 degrees C. These findings suggest that p137 is the mammalian orthologue of Sec31p. (+info)Sec24p and Iss1p function interchangeably in transport vesicle formation from the endoplasmic reticulum in Saccharomyces cerevisiae. (4/342)
The Sec23p/Sec24p complex functions as a component of the COPII coat in vesicle transport from the endoplasmic reticulum. Here we characterize Saccharomyces cerevisiae SEC24, which encodes a protein of 926 amino acids (YIL109C), and a close homologue, ISS1 (YNL049C), which is 55% identical to SEC24. SEC24 is essential for vesicular transport in vivo because depletion of Sec24p is lethal, causing exaggeration of the endoplasmic reticulum and a block in the maturation of carboxypeptidase Y. Overproduction of Sec24p suppressed the temperature sensitivity of sec23-2, and overproduction of both Sec24p and Sec23p suppressed the temperature sensitivity of sec16-2. SEC24 gene disruption could be complemented by overexpression of ISS1, indicating functional redundancy between the two homologous proteins. Deletion of ISS1 had no significant effect on growth or secretion; however, iss1Delta mutants were found to be synthetically lethal with mutations in the v-SNARE genes SEC22 and BET1. Moreover, overexpression of ISS1 could suppress mutations in SEC22. These genetic interactions suggest that Iss1p may be specialized for the packaging or the function of COPII v-SNAREs. Iss1p tagged with His(6) at its C terminus copurified with Sec23p. Pure Sec23p/Iss1p could replace Sec23p/Sec24p in the packaging of a soluble cargo molecule (alpha-factor) and v-SNAREs (Sec22p and Bet1p) into COPII vesicles. Abundant proteins in the purified vesicles produced with Sec23p/Iss1p were indistinguishable from those in the regular COPII vesicles produced with Sec23p/Sec24p. (+info)Sfb2p, a yeast protein related to Sec24p, can function as a constituent of COPII coats required for vesicle budding from the endoplasmic reticulum. (5/342)
The COPII coat is required for vesicle budding from the endoplasmic reticulum (ER), and consists of two heterodimeric subcomplexes, Sec23p/Sec24p, Sec13p/Sec31p, and a small GTPase, Sar1p. We characterized a yeast mutant, anu1 (abnormal nuclear morphology) exhibiting proliferated ER as well as abnormal nuclear morphology at the restrictive temperature. Based on the finding that ANU1 is identical to SEC24, we confirmed a temperature-sensitive protein transport from the ER to the Golgi in anu1-1/sec24-20 cells. Overexpression of SFB2, a SEC24 homologue with 56% identity, partially suppressed not only the mutant phenotype of sec24-20 cells but also rescued the SEC24-disrupted cells. Moreover, the yeast two-hybrid assay revealed that Sfb2p, similarly to Sec24p, interacted with Sec23p. In SEC24-disrupted cells rescued by overexpression of SFB2, some cargo proteins were still retained in the ER, while most of the protein transport was restored. Together, these findings strongly suggest that Sfb2p functions as the component of COPII coats in place of Sec24p, and raise the possibility that each member of the SEC24 family of proteins participates directly and/or indirectly in cargo-recognition events with its own cargo specificity at forming ER-derived vesicles. (+info)Mutants affecting the structure of the cortical endoplasmic reticulum in Saccharomyces cerevisiae. (6/342)
We find that the peripheral ER in Saccharomyces cerevisiae forms a dynamic network of interconnecting membrane tubules throughout the cell cycle, similar to the ER in higher eukaryotes. Maintenance of this network does not require microtubule or actin filaments, but its dynamic behavior is largely dependent on the actin cytoskeleton. We isolated three conditional mutants that disrupt peripheral ER structure. One has a mutation in a component of the COPI coat complex, which is required for vesicle budding. This mutant has a partial defect in ER segregation into daughter cells and disorganized ER in mother cells. A similar phenotype was found in other mutants with defects in vesicular trafficking between ER and Golgi complex, but not in mutants blocked at later steps in the secretory pathway. The other two mutants found in the screen have defects in the signal recognition particle (SRP) receptor. This receptor, along with SRP, targets ribosome-nascent chain complexes to the ER membrane for protein translocation. A conditional mutation in SRP also disrupts ER structure, but other mutants with translocation defects do not. We also demonstrate that, both in wild-type and mutant cells, the ER and mitochondria partially coalign, and that mutations that disrupt ER structure also affect mitochondrial structure. Our data suggest that both trafficking between the ER and Golgi complex and ribosome targeting are important for maintaining ER structure, and that proper ER structure may be required to maintain mitochondrial structure. (+info)Kinase signaling initiates coat complex II (COPII) recruitment and export from the mammalian endoplasmic reticulum. (7/342)
The events regulating coat complex II (COPII) vesicle formation involved in the export of cargo from the endoplasmic reticulum (ER) are unknown. COPII recruitment to membranes is initiated by the activation of the small GTPase Sar1. We have utilized purified COPII components in both membrane recruitment and cargo export assays to analyze the possible role of kinase regulation in ER export. We now demonstrate that Sar1 recruitment to membranes requires ATP. We find that the serine/threonine kinase inhibitor H89 abolishes membrane recruitment of Sar1, thereby preventing COPII polymerization by interfering with the recruitment of the cytosolic Sec23/24 COPII coat complex. Inhibition of COPII recruitment prevents export of cargo from the ER. These results demonstrate that ER export and initiation of COPII vesicle formation in mammalian cells is under kinase regulation. (+info)The p58-positive pre-golgi intermediates consist of distinct subpopulations of particles that show differential binding of COPI and COPII coats and contain vacuolar H(+)-ATPase. (8/342)
We have studied the structural and functional properties of the pre-Golgi intermediate compartment (IC) in normal rat kidney cells using analytical cell fractionation with p58 as the principal marker. The sedimentation profile (sediterm) of p58, obtained by analytical differential centrifugation, revealed in steady-state cells the presence of two main populations of IC elements whose average sedimentation coefficients, s(H)=1150+/-58S ('heavy') and s(L)=158+/-8S ('light'), differed from the s-values obtained for elements of the rough and smooth endoplasmic reticulum. High resolution analysis of these subpopulations in equilibrium density gradients further revealed that the large difference in their s-values was mainly due to particle size. The 'light' particle population contained the bulk of COPI and COPII coats, and redistribution of p58 to these particles was observed in transport-arrested cells, showing that the two types of elements are also compositionally distinct and have functional counterparts in intact cells. Using a specific antibody against the 16 kDa proteolipid subunit of the vacuolar H(+)-ATPase, an enrichment of the V(o )domain of the ATPase was observed in the p58-positive IC elements. Interestingly, these elements could contain both COPI and COPII coats and their density distribution was markedly affected by GTP(&ggr;)S. Together with morphological observations, these results demonstrate that, in addition to clusters of small tubules and vesicles, the IC also consists of large-sized structures and corroborate the proposal that the IC elements contain an active vacuolar H(+)-ATPase. (+info)
Melanoma inhibitory activity protein 3
CTAGE5 protein (human) - STRING interaction network
Concentration of Sec12 at ER exit sites via interaction with cTAGE5 is required for collagen export | JCB
PCTAIRE protein kinases interact directly with the COPII complex and modulate secretory cargo transport<...
Nucleation of COPII Vesicular Coat Complex by Endoplasmic Reticulum to Golgi Vesicle SNAREs | Science
ER-to-Golgi transport by COPII vesicles in Arabidopsis involves a ribosome-excluding scaffold that is transferred with the...
Bcr (breakpoint cluster region) protein binds to PDZ-domains of scaffold protein PDZK1 and vesicle coat protein Mint3 | Journal...
Leica Science Lab - Tag : Cell Biology
Sec31a - Protein transport protein Sec31A - Rattus norvegicus (Rat) - Sec31a gene & protein
COPs are held up by Sec16p | JCB
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SEC13 (YLR208W) Result Summary | BioGRID
HIPHOP chemogenomics database
HIPHOP chemogenomics database
v4/tir/gdpr/export/{site uuid}/{thirdparty id}/{thirdparty provider}
dcyphr | COPII: A Membrane Coat Formed by Sec Proteins That Drive Vesicle Budding from the Endoplasmic Reticulum.
Sec23a SEC23 homolog A, COPII coat complex component [Mus musculus (house mouse)] - Gene - NCBI
ERP2 (YAL007C) Result Summary | BioGRID
E (exit) site synonyms, E (exit) site antonyms - FreeThesaurus.com
Sec16A restarts secretion post mitosis | Journal of Cell Science
Roles of singleton tryptophan motifs in COPI coat stability and vesicle tethering. | Department of Molecular Biology
A model for the self-organization of exit sites in the endoplasmic reticulum. - Semantic Scholar
Publications | Max Planck Institute for Biophysical Chemistry
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Molecular evolution of the vesicle coat component betaCOP in Toxoplasma gondii.
ARF-GAP-mediated interaction between the ER-Golgi v-SNAREs and the COPI coat :: MPG.PuRe
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TumorPortal
TumorPortal
Tethering assays for COPI vesicles mediated by golgins<...
Role of Erv14p in vesicle mediated protein transport from the endoplasmic reticulum to the Golgi in Saccharomyces cerevisiae. :...
IJMS | Free Full-Text | ARF1 and SAR1 GTPases in Endomembrane Trafficking in Plants | HTML
Patent US8092909 - Color foundation coat and color top coat paint system - Google Patents
ZNF624 contains 21 C2H2-type zinc fingers through which it is thought tobe involved in DNA-binding and transcriptional...
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Golgi and Protein Processing
Giulia Zanettis story - EMBL Imaging Centre
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Characterization of COPII vesicle proteins involved in sorting between the endoplasmic reticulum and golgi apparatus in Sacch[a...
Pdcd6 - Programmed cell death protein 6 - Rattus norvegicus (Rat) - Pdcd6 gene & protein
The cell. 5. Vesicular trafficking. From the reticulum to the Golgi. Atlas of plant and animal histology.
Golgi body
Institut de Biologie Intégrative de la Cellule
The Endoplasmic Reticulum and Golgi Body: Whats the Difference?
Canberra. ACT | Australian Abattoirs
Finnair Cargo partners with RTS -- RTS Corp | PRLog
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Red Nissan NV Cargo | Nissan USA
Sec Form 4 Filing - Feeler Jeffrey R @ US ECOLOGY, INC. - 2017-03-13
NEW 560 SEC...NEW PROBLEMS | Mercedes-Benz Forum
1 - Agea forex online
Coatomer
... β-COP), Sec21 (γ-COP), Ret2(δ-COP), and Ret3 (ζ-COP). COPI is a coatomer that coats the vesicles transporting proteins from the ... COP1 coated vesicles also contain p24 proteins that assist with cargo sorting. COP II is a coatomer that coats the vesicles ... This complex polymerizes to form the outer layer of the coat. COP II vesicles must shed their coat before they can fuse with ... Once the vesicle is coated, it begins to travel to the ER. Before the vesicle can fuse with the ER membrane, the coats ...
COPI
"ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein ... Sönnichsen B, Watson R, Clausen H, Misteli T, Warren G (1996). "Sorting by COP I-coated vesicles under interphase and mitotic ... COPII vesicles Clathrin vesicles Glyceraldehyde 3-phosphate dehydrogenase#ER to Golgi transport Exomer Coat+Protein+Complex+I ... A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly". Science. 349 (6244): 195-198. doi: ...
Beta2-adaptin C-terminal domain
McMahon HT, Mills IG (August 2004). "COP and clathrin-coated vesicle budding: different pathways, common approaches". Curr. ... AP (adaptor protein) complexes are found in coated vesicles and clathrin-coated pits. AP complexes connect cargo proteins and ... and from there via small carrier vesicles to their final destination compartment. These vesicles have specific coat proteins ( ... This is an adaptor protein which helps the formation of a clathrin coat around a vesicle. This entry represents a subdomain of ...
Clathrin adaptor protein
"COP and clathrin-coated vesicle budding: different pathways, common approaches". Curr. Opin. Cell Biol. 16 (4): 379-91. doi: ... Adaptor protein (AP) complexes are found in coated vesicles and clathrin-coated pits. AP complexes connect cargo proteins and ... Clathrin coats contain both clathrin (acts as a scaffold) and adaptor complexes that link clathrin to receptors in coated ... lipids to clathrin at vesicle budding sites, as well as binding accessory proteins that regulate coat assembly and disassembly ...
Vesicular transport protein
COP 1 (Cytosolic coat protein complex ) : retrograde transport; Golgi ----> Endoplasmic reticulum COP 2 (Cytosolic coat protein ... As a result, vesicular transporters govern the concentration of molecules within a vesicle. Examples include: Archain ARFs ... is a membrane protein that regulates or facilitates the movement of specific molecules across a vesicle's membrane. ... each using its own coat and GTPase. ...
COPB1
1991). "Beta-COP, a 110 kd protein associated with non-clathrin-coated vesicles and the Golgi complex, shows homology to beta- ... "Physical interaction of the HIV-1 Nef protein with beta-COP, a component of non-clathrin-coated vesicles essential for membrane ... Orcl L, Palmer DJ, Amherdt M, Rothman JE (1993). "Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins ... Lowe M, Kreis TE (1997). "In vivo assembly of coatomer, the COP-I coat precursor". J. Biol. Chem. 271 (48): 30725-30. doi: ...
Brefeldin A
Activated Arf1p then recruits coat protein β-COP, a subunit of the COP-I complex, to cargo-bound receptors on the membrane. ... of SNARE proteins in the Golgi which would otherwise be bound to coat protein-coated vesicles and removed with the vesicles ... Coat protein recruitment is necessary for proper vesicle formation and transport. Brefeldin A reversibly inhibits the function ... protein transport from the endoplasmic reticulum to the golgi complex indirectly by preventing association of COP-I coat to the ...
ARF1
1991). "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding ... Eugster A, Frigerio G, Dale M, Duden R (August 2000). "COP I domains required for coatomer integrity, and novel interactions ... "Site-specific photocrosslinking to probe interactions of Arf1 with proteins involved in budding of COPI vesicles". Methods. 20 ...
Endocytosis
Coat complexes that have been well characterized so far include coat protein-I (COP-I), COP-II, and clathrin. Clathrin coats ... Coats function to deform the donor membrane to produce a vesicle, and they also function in the selection of the vesicle cargo ... Clathrin-coated vesicles (CCVs) are found in virtually all cells and form domains of the plasma membrane termed clathrin-coated ... Coated vesicles were first purified by Barbara Pearse, who discovered the clathrin coat molecule in 1976. Caveolin proteins ...
Halperin-Birk syndrome
The repertoire of COP-II paralogs available in mammals could contribute to a wide variety of COP-II coats, thus facilitating ... The COP-II complex comprises five highly conserved proteins, among these SEC31A, creating small membrane vesicles that ... Alternative splicing could further contribute to the COP-II vesicle and cargo selection diversity. CRISPR/Cas9-mediated ... the extruded membrane is separated from the ER membrane to form an intact vesicle. Most mammalian COP-II complex subunits have ...
COP1
For the membrane coated vesicle used in transport, see here. Fagol Caspase recruitment domain-containing protein 16 is an ... Lee SH, Stehlik C, Reed JC (Sep 2001). "Cop, a caspase recruitment domain-containing protein and inhibitor of caspase-1 ... 2006). "Protective role of Cop in Rip2/caspase-1/caspase-4-mediated HeLa cell death". Biochim. Biophys. Acta. 1762 (8): 742-54 ...
List of MeSH codes (A11)
... clathrin-coated vesicles MeSH A11.284.430.214.190.875.190.880.180.180 - cop-coated vesicles MeSH A11.284.430.214.190.875. ... transport vesicles MeSH A11.284.430.214.190.875.190.880.180 - coated vesicles MeSH A11.284.430.214.190.875.190.880.180.160 - ... 190.880.810 - secretory vesicles MeSH A11.284.430.214.190.875.190.880.830 - synaptic vesicles MeSH A11.284.430.214.190.875. ... coated pits, cell-membrane MeSH A11.284.149.165.175.160 - caveolae MeSH A11.284.149.165.355 - glycocalyx MeSH A11.284.149.165. ...
COPG2
Lowe M, Kreis TE (1997). "In vivo assembly of coatomer, the COP-I coat precursor". J. Biol. Chem. 271 (48): 30725-30. doi: ... a cytosolic protein complex containing subunits of non-clathrin-coated Golgi transport vesicles". Nature. 349 (6306): 248-51. ... Bermak JC, Li M, Bullock C, Weingarten P, Zhou QY (2002). "Interaction of gamma-COP with a transport motif in the D1 receptor C ... Yamasaki K, Hayashida S, Miura K, Masuzaki H, Ishimaru T, Niikawa N, Kishino T (Nov 2000). "The novel gene, gamma2-COP (COPG2 ...
Jean Gruenberg
COP coat proteins, the N-ethylmaleimide sensitive factor, the small transmembrane proteins of the p24 family, the p38 MAP ... "An endosomal beta COP is involved in the pH-dependent formation of transport vesicles destined for late endosomes". The Journal ... "Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle fusion in ... Gruenberg, J. E.; Howell, K. E. (1986-12-01). "Reconstitution of vesicle fusions occurring in endocytosis with a cell-free ...
COPE (gene)
Orcl L, Palmer DJ, Amherdt M, Rothman JE (1993). "Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins ... Eugster A, Frigerio G, Dale M, Duden R (2000). "COP I domains required for coatomer integrity, and novel interactions with ARF ... is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles ... Eugster A, Frigerio G, Dale M, Duden R (August 2000). "COP I domains required for coatomer integrity, and novel interactions ...
COPG
It is one of seven proteins in the COPI coatomer complex that coats vesicles as they bud from the Golgi complex. COPG has been ... Lowe, M; Kreis T E (November 1996). "In vivo assembly of coatomer, the COP-I coat precursor". J. Biol. Chem. UNITED STATES. 271 ... Lowe M, Kreis TE (1997). "In vivo assembly of coatomer, the COP-I coat precursor". J. Biol. Chem. 271 (48): 30725-30. doi: ... 2000). "Duplication of genes encoding non-clathrin coat protein gamma-COP in vertebrate, insect and plant evolution". FEBS Lett ...
COPA (gene)
Chow VT, Quek HH (1997). "Alpha coat protein COPA (HEP-COP): presence of an Alu repeat in cDNA and identity of the amino ... Orcl L, Palmer DJ, Amherdt M, Rothman JE (1993). "Coated vesicle assembly in the Golgi requires only coatomer and ARF proteins ... The subunits are designated alpha-COP, beta-COP, beta-prime-COP, gamma-COP, delta-COP, epsilon-COP, and zeta-COP. The alpha-COP ... Lowe M, Kreis TE (November 1996). "In vivo assembly of coatomer, the COP-I coat precursor". J. Biol. Chem. 271 (48): 30725-30. ...
COPB2
The Golgi coatomer complex (see MIM 601924) constitutes the coat of nonclathrin-coated vesicles and is essential for Golgi ... Lowe M, Kreis TE (November 1996). "In vivo assembly of coatomer, the COP-I coat precursor". J. Biol. Chem. 271 (48): 30725-30. ... Lowe M, Kreis TE (1997). "In vivo assembly of coatomer, the COP-I coat precursor". J. Biol. Chem. 271 (48): 30725-30. doi: ... Stenbeck G, Harter C, Brecht A, Herrmann D, Lottspeich F, Orci L, Wieland FT (1993). "beta'-COP, a novel subunit of coatomer". ...
Alpha solenoid
Vesicle coat proteins frequently contain alpha solenoids and share common domain architecture with some NPC proteins. Three ... Field, Mark C.; Sali, Andrej; Rout, Michael P. (13 June 2011). "On a bender-BARs, ESCRTs, COPs, and finally getting your coat ... vesicle coat proteins, and nuclear pore complexes". Current Opinion in Cell Biology. 21 (1): 4-13. doi:10.1016/j.ceb.2008.12. ... and Clathrin Vesicle Coats". Cell. 142 (1): 123-132. doi:10.1016/j.cell.2010.05.030. PMC 2943847. PMID 20579721. Forwood, Jade ...
Vesicular transport adaptor protein
The best characterized type of vesicle is the clathrin coated vesicle (CCV). The formation of a COPII vesicle at the ... Components of COPI (cop one) a coatomer, and TSET (T-set) a membrane trafficking complex have similar heterotetramers of the AP ... but the coat of COPI is not closely related to the coats of either CCVs or COPII vesicles. AP-5 is associated with 2 proteins, ... but the ultrastructure of that coat is not known. The coat of AP-4 is unknown. An almost universal feature of coat assembly is ...
Golgi apparatus
Cargo then progress toward the trans face in COPI-coated vesicles. This model proposes that COPI vesicles move in two ... ARFs are small GTPases which regulate vesicular trafficking through the binding of COPs to endosomes and the Golgi. BFA ... Proteins are delivered from the ER to the cis face using COPII-coated vesicles. ... Once matured, the TGN cisternae dissolve to become secretory vesicles. While this progression occurs, COPI vesicles continually ...
DeCS 2008 - versión 17 de Marzo de 2008
ARCN1 archain 1 [Homo sapiens (human)] - Gene - NCBI
part_of COPI vesicle coat IBA Inferred from Biological aspect of Ancestor. more info ... Crystallization and preliminary X-ray analysis of the C-terminal domain of δ-COP, a medium-sized subunit of the COPI complex ... part_of COPI vesicle coat ISS Inferred from Sequence or Structural Similarity. more info ... COPI coat complex subunit delta. archain vesicle transport protein 1. coatomer delta subunit. coatomer protein complex, subunit ...
DeCS - New terms
COP-COATED VESICLES. VESICULAS CUBIERTAS DE PCO. VESÍCULAS COBERTAS PELO COMPLEXO DE PROTEÍNA DO ENVOLTÓRIO. ... CLATHRIN-COATED VESICLES. VESICULAS CUBIERTAS DE CLATRINA. VESÍCULAS COBERTAS DE CLATRINA. COMMUNICABLE DISEASES, EMERGING. ... SECRETORY VESICLES. VESICULAS SECRETORAS. VESÍCULAS SECRETÓRIAS. SELF-SUSTAINED SEQUENCE REPLICATION. REPLICACION DE SECUENCIA ...
DeCS - Términos Nuevos
DeCS - Termos Novos
DeCS - Termos Novos
DeCS - New terms
COP-COATED VESICLES. VESICULAS CUBIERTAS DE PCO. VESÍCULAS COBERTAS PELO COMPLEXO DE PROTEÍNA DO ENVOLTÓRIO. ... CLATHRIN-COATED VESICLES. VESICULAS CUBIERTAS DE CLATRINA. VESÍCULAS COBERTAS DE CLATRINA. COMMUNICABLE DISEASES, EMERGING. ... SECRETORY VESICLES. VESICULAS SECRETORAS. VESÍCULAS SECRETÓRIAS. SELF-SUSTAINED SEQUENCE REPLICATION. REPLICACION DE SECUENCIA ...
DeCS - Termos Novos
DeCS - Termos Novos
DeCS - Términos Nuevos
DeCS - New terms
COP-COATED VESICLES. VESICULAS CUBIERTAS DE PCO. VESÍCULAS COBERTAS PELO COMPLEXO DE PROTEÍNA DO ENVOLTÓRIO. ... CLATHRIN-COATED VESICLES. VESICULAS CUBIERTAS DE CLATRINA. VESÍCULAS COBERTAS DE CLATRINA. COMMUNICABLE DISEASES, EMERGING. ... SECRETORY VESICLES. VESICULAS SECRETORAS. VESÍCULAS SECRETÓRIAS. SELF-SUSTAINED SEQUENCE REPLICATION. REPLICACION DE SECUENCIA ...
DeCS - New terms
COP-COATED VESICLES. VESICULAS CUBIERTAS DE PCO. VESÍCULAS COBERTAS PELO COMPLEXO DE PROTEÍNA DO ENVOLTÓRIO. ... CLATHRIN-COATED VESICLES. VESICULAS CUBIERTAS DE CLATRINA. VESÍCULAS COBERTAS DE CLATRINA. COMMUNICABLE DISEASES, EMERGING. ... SECRETORY VESICLES. VESICULAS SECRETORAS. VESÍCULAS SECRETÓRIAS. SELF-SUSTAINED SEQUENCE REPLICATION. REPLICACION DE SECUENCIA ...
DeCS - Termos Novos
DeCS - New terms
COP-COATED VESICLES. VESICULAS CUBIERTAS DE PCO. VESÍCULAS COBERTAS PELO COMPLEXO DE PROTEÍNA DO ENVOLTÓRIO. ... CLATHRIN-COATED VESICLES. VESICULAS CUBIERTAS DE CLATRINA. VESÍCULAS COBERTAS DE CLATRINA. COMMUNICABLE DISEASES, EMERGING. ... SECRETORY VESICLES. VESICULAS SECRETORAS. VESÍCULAS SECRETÓRIAS. SELF-SUSTAINED SEQUENCE REPLICATION. REPLICACION DE SECUENCIA ...
DeCS - Términos Nuevos
DeCS - Termos Novos
DeCS - New terms
COP-COATED VESICLES. VESICULAS CUBIERTAS DE PCO. VESÍCULAS COBERTAS PELO COMPLEXO DE PROTEÍNA DO ENVOLTÓRIO. ... CLATHRIN-COATED VESICLES. VESICULAS CUBIERTAS DE CLATRINA. VESÍCULAS COBERTAS DE CLATRINA. COMMUNICABLE DISEASES, EMERGING. ... SECRETORY VESICLES. VESICULAS SECRETORAS. VESÍCULAS SECRETÓRIAS. SELF-SUSTAINED SEQUENCE REPLICATION. REPLICACION DE SECUENCIA ...
Background
COP and clathrin-coated vesicle budding: different pathways, common approaches. . Curr Opin Cell Biol. . 2004. ;. 16. (. 4. ): ... Extracellular vesicles are derived from the multivesicular body and act in host/parasite relationships and cell-cell signaling ... Parasite extracellular vesicles: mediators of intercellular communication. . PLoS Pathog. . 2014. ;. 10. (. 8. ):. e1004289. ... Cell-cell communication between malaria-infected red blood cells via exosome-like vesicles. . Cell. . 2013. ;. 153. (. 5. ):. ...
Browsing by Subject "Alpha 1-COP"
EN] COP (coat protein) I-coated vesicles mediate intra-Golgi transport and retrograde transport from the Golgi to the ... alpha 2-COP is involved in early secretory traffic in Arabidopsis and is required for plant growth ... These vesicles form through the action of the small GTPase ADP-ribosylation ... ... Browsing by Subject "Alpha 1-COP". RiuNet: Institutional repository of the Polithecnic University of Valencia. ...
Human ARF4 expression rescues sec7 mutant yeast cells - Wikidata
ADP-Ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: A novel role for a GTP-binding protein ... Hydrolysis of bound GTP by ARF protein triggers uncoating of Golgi-derived COP-coated vesicles ... Cytosolic ARFs are required for vesicle formation but not for cell-free intra-Golgi transport: evidence for coated vesicle- ... COPII: a membrane coat formed by Sec proteins that drive vesicle budding from the endoplasmic reticulum ...
CoP: Co-expressed Biological Processes
ER to Golgi vesicle-mediated transport;C:COPI vesicle coat, CUL4 RING ubiquitin ligase complex, membrane;MFOBPA. S.X.. H.G.. ... coatomer gamma-2 subunit, putative / gamma-2 coat protein, putative / gamma-2 COP, putative. F:protein binding, clathrin ... Back to the CoP portal site. Back to the KAGIANA project homepage. ... F:protein binding, structural molecule activity, transporter activity;P:intracellular protein transport, vesicle-mediated ...
Gpr63 is a modifier of microcephaly in Ttc21b... | proLékárníky.cz
GO:0030126: COPI vesicle coat details
YOR184W 2167.216480 INESSENTIAL SER1 phosphoserine transaminase,phosphoserine aminotransferase
4.864154 INESSENTIAL SEC28 epsilon-COP coatomer subunit Sec28p, non-selective vesicle coating, vesicle transport, coatomer ... vesicle transport, vesicle transport, coated vesicle YKR007W 0.116917 INESSENTIAL biological_process unknown, molecular_ ... YML012W 14.541695 INESSENTIAL ERV25 vesicle coat component YLR271W 14.508198 INESSENTIAL biological_process unknown, molecular_ ... non-selective vesicle fusion*, v-SNARE, inter-Golgi transport vesicle* YHR009C -2.349478 INESSENTIAL biological_process unknown ...
Búsqueda | Portal Regional de la BVS
Dsl1 tethering complex | SGD
Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. ... Schmitt HD (2010) Dsl1p/Zw10: common mechanisms behind tethering vesicles and microtubules. Trends Cell Biol 20(5):257-68 PMID ... 2009) Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol 16( ...
The Golgi apparatus: balancing new with old. | Profiles RNS
Search Results | Biochemical Society Transactions | Portland Press
ER/Golgi intermediate compartment COP, coat protein of non-clathrin-coated vesicles... ... homology β-COP, β-coat protein MAD, membrane association domain GST, glutathione S-transferase Btk, Brutons tyrosine kinase ... COP, coatomer protein ER, endoplasmic reticulum GA, Golgi apparatus GFP, green fluorescent protein VTC, vesicular-tubular ...
CD4 downregulation by the HIV-1 protein Nef reveals distinct roles for the γ1 and γ2 subunits of the AP-1 complex in protein...
Phospholipase D is involved in the formation of Golgi associated clathrin coated vesicles in human parotid duct cells ... HIV-1 Nef targets MHC-I and CD4 for degradation via a final common beta-COP-dependent pathway in T cells ... that induces the endocytosis of CD4 mediated by clathrin-coated vesicles (CCVs) (Burtey et al., 2007; Greenberg et al., 1997). ... Shedding of ciliary vesicles at a glance. Check out our latest Cell Science at a Glance article and accompanying poster for an ...
Golgi4
- EN] COP (coat protein) I-coated vesicles mediate intra-Golgi transport and retrograde transport from the Golgi to the endoplasmic reticulum. (upv.es)
- Vesicles with COPI coats are found associated with Golgi membranes at steady state. (ntu.edu.sg)
- W. E. ( I 998) Trends Received 2 March 2000 0 2000 Biochemical Society 512 green fluorescent protein mutagenesis, Sar1 COP, coatomer protein ER , endoplasmic reticulum GA, Golgi apparatus GFP, green fluorescent protein VTC, vesicular-tubular clusters or intermediate. (silverchair.com)
- Because both Arf and PLD1 stimulate vesicle formation in the Golgi, these data raise the possibility that vesicle formation and trafficking may play a role in the transduction of intracellular signals. (embl.de)
COPI2
- Crystallization and preliminary X-ray analysis of the C-terminal domain of δ-COP, a medium-sized subunit of the COPI complex involved in membrane trafficking. (nih.gov)
- Hsia KC and Hoelz A (2010) Crystal structure of alpha-COP in complex with epsilon-COP provides insight into the architecture of the COPI vesicular coat. (yeastgenome.org)
Membrane2
- One of two multimeric complexes that forms a membrane vesicle coat. (ntu.edu.sg)
- Activation of phospholipase D1 (PLD1) by Arf has been implicated in vesicle transport and membrane trafficking. (embl.de)
Protein1
- Transient N-glycosylation abnormalities likely due to a de novo loss-of-function mutation in the delta subunit of coat protein I. Reunert J, et al . (nih.gov)
Subunits1
- Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. (yeastgenome.org)
Membranes1
- That such enzymes are formed in the protoplasm is evident from the behaviour of hyphae, which have been observed to pierce cell-membranes, the chitinous coats of insects, artificial collodion films and layers of wax, &c. (yourdictionary.com)
Clathrin1
- It has similarities to heat shock proteins and clathrin-associated proteins, and may be involved in vesicle structure or trafficking. (nih.gov)
Secretory1
- The role of coat proteins in the biosynthesis of secretory proteins. (wikidata.org)
Empty2
- Equally, how can viral researchers know that they are not detecting similarly sized non-viral vesicles or empty vectors during vaccine production? (viroliegy.com)
- ACG is one of the largest manufacturer of empty hard capsules in the world, offering a complete range of solutions beginning with empty capsules, granulation and coating, capsule filling, tabletting, packaging films, blister packing and carton packing, to end-of-line solutions. (ondrugdelivery.com)
Films1
- Films of pyrites sometimes coat the joint-planes of coal. (yourdictionary.com)
Network1
- Herein, a zeolitic imidazolate framework derived amorphous CoP combined with carbon nanotubes conductive network composites ([email protected]) has been synthesized as an effective dual-electrocatalyst for accelerating the redox kinetics of polysulfides to prolong the lifespan of Li-S batteries. (energymaterj.com)
COPII9
- How cells unify and organize the curvature-generating factors at the nanoscale is presented for three ubiquitous coats central for membrane trafficking in eukaryotes: clathrin-coated pits, caveolae, and COPI and COPII coats. (nature.com)
- The Sar1 family of proteins initiates the intracellular transport of proteins in COPII (coat protein)-coated vesicles. (nih.gov)
- Our data suggest that chylomicrons, which vastly exceed the size of typical COPII vesicles, are selectively recruited by the COPII machinery for transport through the secretory pathways of the cell. (nih.gov)
- COPII coat assembly and selective export from the endoplasmic reticulum. (nih.gov)
- A structural view of the COPII vesicle coat. (nih.gov)
- The outer surface of these vesicles is covered with a lattice-like network of COP (coat protein complex) proteins, either COPI or COPII. (nih.gov)
- COPI coated vesicles transport backwards from the cisternae of the GOLGI APPARATUS to the rough endoplasmic reticulum ( ENDOPLASMIC RETICULUM, ROUGH ), while COPII coated vesicles transport forward from the rough endoplasmic reticulum to the Golgi apparatus. (nih.gov)
- This process is mediated by the coat protein complex II (COPII) machinery, which at the minimum, comprises the Sar1 GTPase and the cytosolic protein complexes Sec23/Sec24 (Sec23/24) and Sec13/Sec31 (Sec13/31). (scripps.edu)
- We after that asked if the COPII vesicle program that mediates trafficking in the ER towards the Golgi includes a function in Golgi biogenesis. (angiogenesis-blog.com)
Proteins9
- We propose that the integration of individual mechanisms into a highly controlled, robust process of curvature generation often relies on the assembly of proteins into coats. (nature.com)
- Symmetrical arrangement of proteins under release-ready vesicles in presynaptic terminals. (academictree.org)
- This traffic is bidirectional, to ensure that proteins required to form vesicles are recycled. (embl.de)
- Activated small guanine triphosphatases (GTPases) attract coat proteins to specific membrane export sites, thereby linking coatomers to export cargos. (embl.de)
- As coat proteins polymerise, vesicles are formed and budded from membrane-bound organelles. (embl.de)
- A class of proteins involved in the transport of molecules via TRANSPORT VESICLES. (lookformedical.com)
- Some portray biological membranes as molecular putty readily fashioned into different shapes (vesicles, tubes, and sheets) by proteins or protein complexes. (nih.gov)
- These proteins, called ADP-ribosylation factors, or Arfs, have recently been found to 1) interact with specific lipid components of membranes, 2) modify the lipid composition and release potential second messengers through activation of phospholipase D (PLD), and 3) regulate the assembly of at least a subset of protein complexes or membrane coats (for reviews, see refs. (nih.gov)
- AP complexes connect cargo proteins and lipids to clathrin at vesicle budding sites, as well as binding accessory proteins that regulate coat assembly and disassembly (such as AP180, epsins and auxilin). (xfam.org)
Endoplasmic reticulum1
- Membrane traffic along the eukaryotic secretory pathway starts with the selective packing of biosynthetic cargo into nascent vesicles that are forming on the endoplasmic reticulum (ER). (scripps.edu)
CELL-MEMBRANE2
- TRANSPORT VESICLES formed when cell-membrane coated pits ( COATED PITS, CELL-MEMBRANE ) invaginate and pinch off. (nih.gov)
- An adaptor protein complex primarily involved in the formation of clathrin-related endocytotic vesicles (ENDOSOMES) at the CELL MEMBRANE. (lookformedical.com)
Pits1
- AP (adaptor protein) complexes are found in coated vesicles and clathrin-coated pits. (xfam.org)
Extracellular1
- This review highlights advances in magnetic labeling of both cells and extracellular vesicles, which is one of the hottest branches in biology. (freewechat.com)
Receptors1
- Clathrin coats contain both clathrin (acts as a scaffold) and adaptor complexes that link clathrin to receptors in coated vesicles. (xfam.org)
Intracellular transport1
- The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. (lookformedical.com)
Membranes1
- The emerging theme is that these coats arrange and coordinate curvature-generating factors in time and space to dynamically shape membranes to accomplish membrane trafficking within cells. (nature.com)
Pinch off1
- Using similar examples and Mary, Seat writing services you havent on the power of persons of to be and value skill, you pinch off and who vesicle, and. (lsp-poltekapp.org)
Coatomer1
- The zeta subunit may be involved in regulating the coat assembly and, hence, the rate of biosynthetic protein transport due to its association-dissociation properties with the coatomer complex (By similarity). (nih.gov)
Mechanisms1
- Dsl1p/Zw10: common mechanisms behind tethering vesicles and microtubules. (mpg.de)
Complex1
- A tethering complex recruits SNAREs and grabs vesicles. (mpg.de)
Cells1
- FRAP evaluation revealed which the mEGFPCTb-COP indication recovered quickly in uninduced cells (= 15 for (C) TbArf1-3Tcon1 [Q71L], = 11 for (+) TbArf1-3Tcon1 [Q71L]. (angiogenesis-blog.com)
Assembly1
- Vesicle capture by membrane-bound Munc13-1 requires self-assembly into discrete clusters. (academictree.org)
Main1
- Evaluation from the inhibitory information shows that in early endosomes and endocytic vesicles NHE3 can be of main importance, whereas plasma membrane NHE3 takes on a minor part. (thetechnoant.info)
Link1
- A link between ER tethering and COP-I vesicle uncoating. (mpg.de)
Early1
- Analysis of the inhibitory profiles indicates that in early endosomes and endocytic vesicles NHE3 is usually of major importance, whereas plasma VX-745 membrane NHE3 plays a minor role. (ncbedbugs.com)