Stigmatella: A genus of the MYXOCOCCALES having vegetative cells which are straight rods with tapered ends and myxospores which are short and somewhat crooked. Fruiting bodies consist of spherical, ovoid, or club-shaped sporangioles on stalks. (From Bergey's Manual of Determinative Bacteriology, 9th ed)Glyceraldehyde-3-Phosphate Dehydrogenase (Phosphorylating): An NAD-dependent glyceraldehyde-3-phosphate dehydrogenase found in the cytosol of eucaryotes. It catalyses the dehydrogenation and phosphorylation of GLYCERALDEHYDE 3-PHOSPHATE to 3-phospho-D-glyceroyl phosphate, which is an important step in the GLYCOLYSIS pathway.Recombination, Genetic: Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.Gene Library: A large collection of DNA fragments cloned (CLONING, MOLECULAR) from a given organism, tissue, organ, or cell type. It may contain complete genomic sequences (GENOMIC LIBRARY) or complementary DNA sequences, the latter being formed from messenger RNA and lacking intron sequences.Genetic Engineering: Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.Promoter Regions, Genetic: DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Anthozoa: A class in the phylum CNIDARIA, comprised mostly of corals and anemones. All members occur only as polyps; the medusa stage is completely absent.Coral Reefs: Marine ridges composed of living CORALS, coral skeletons, calcareous algae, and other organisms, mixed with minerals and organic matter. They are found most commonly in tropical waters and support other animal and plant life.Dinoflagellida: Flagellate EUKARYOTES, found mainly in the oceans. They are characterized by the presence of transverse and longitudinal flagella which propel the organisms in a rotating manner through the water. Dinoflagellida were formerly members of the class Phytomastigophorea under the old five kingdom paradigm.Symbiosis: The relationship between two different species of organisms that are interdependent; each gains benefits from the other or a relationship between different species where both of the organisms in question benefit from the presence of the other.Acclimatization: Adaptation to a new environment or to a change in the old.Cnidaria: A phylum of radially symmetrical invertebrates characterized by possession of stinging cells called nematocysts. It includes the classes ANTHOZOA; CUBOZOA; HYDROZOA, and SCYPHOZOA. Members carry CNIDARIAN VENOMS.Oligonucleotide Array Sequence Analysis: Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.Plumbaginaceae: A plant family of the order Plumbaginales, subclass Caryophyllidae, class Magnoliopsida of shrubs and herbs. Some members contain ANTHOCYANINS and naphthaquinones.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Repressor Proteins: Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.NFATC Transcription Factors: A family of transcription factors characterized by the presence of highly conserved calcineurin- and DNA-binding domains. NFAT proteins are activated in the CYTOPLASM by the calcium-dependent phosphatase CALCINEURIN. They transduce calcium signals to the nucleus where they can interact with TRANSCRIPTION FACTOR AP-1 or NF-KAPPA B and initiate GENETIC TRANSCRIPTION of GENES involved in CELL DIFFERENTIATION and development. NFAT proteins stimulate T-CELL activation through the induction of IMMEDIATE-EARLY GENES such as INTERLEUKIN-2.Transcription, Genetic: The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.RNA Polymerase II: A DNA-dependent RNA polymerase present in bacterial, plant, and animal cells. It functions in the nucleoplasmic structure and transcribes DNA into RNA. It has different requirements for cations and salt than RNA polymerase I and is strongly inhibited by alpha-amanitin. EC 2.7.7.6.Chromatin: The material of CHROMOSOMES. It is a complex of DNA; HISTONES; and nonhistone proteins (CHROMOSOMAL PROTEINS, NON-HISTONE) found within the nucleus of a cell.Histones: Small chromosomal proteins (approx 12-20 kD) possessing an open, unfolded structure and attached to the DNA in cell nuclei by ionic linkages. Classification into the various types (designated histone I, histone II, etc.) is based on the relative amounts of arginine and lysine in each.Molecular Biology: A discipline concerned with studying biological phenomena in terms of the chemical and physical interactions of molecules.Blood DonorsLymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Advertising as Topic: The act or practice of calling public attention to a product, service, need, etc., especially by paid announcements in newspapers, magazines, on radio, or on television. (Random House Unabridged Dictionary, 2d ed)Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.BostonHuntington Disease: A familial disorder inherited as an autosomal dominant trait and characterized by the onset of progressive CHOREA and DEMENTIA in the fourth or fifth decade of life. Common initial manifestations include paranoia; poor impulse control; DEPRESSION; HALLUCINATIONS; and DELUSIONS. Eventually intellectual impairment; loss of fine motor control; ATHETOSIS; and diffuse chorea involving axial and limb musculature develops, leading to a vegetative state within 10-15 years of disease onset. The juvenile variant has a more fulminant course including SEIZURES; ATAXIA; dementia; and chorea. (From Adams et al., Principles of Neurology, 6th ed, pp1060-4)TATA-Box Binding Protein: A general transcription factor that plays a major role in the activation of eukaryotic genes transcribed by RNA POLYMERASES. It binds specifically to the TATA BOX promoter element, which lies close to the position of transcription initiation in RNA transcribed by RNA POLYMERASE II. Although considered a principal component of TRANSCRIPTION FACTOR TFIID it also takes part in general transcription factor complexes involved in RNA POLYMERASE I and RNA POLYMERASE III transcription.MassachusettsNew YorkRoot Cause Analysis: Multi-step systematic review process used for improving safety by investigation of incidents to find what happened, why it happened, and to determine what can be done to prevent it from happening again.Sp1 Transcription Factor: Promoter-specific RNA polymerase II transcription factor that binds to the GC box, one of the upstream promoter elements, in mammalian cells. The binding of Sp1 is necessary for the initiation of transcription in the promoters of a variety of cellular and viral GENES.Saccharomyces cerevisiae: A species of the genus SACCHAROMYCES, family Saccharomycetaceae, order Saccharomycetales, known as "baker's" or "brewer's" yeast. The dried form is used as a dietary supplement.Tristetraprolin: A ZINC FINGER MOTIF containing transcription factor that was originally identified as one of the IMMEDIATE-EARLY PROTEINS. It shuttles between the CYTOPLASM and the CELL NUCLEUS and is involved in destabilization of mRNAs for TUMOR NECROSIS FACTOR-ALPHA.Iron: A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Saccharomyces cerevisiae Proteins: Proteins obtained from the species SACCHAROMYCES CEREVISIAE. The function of specific proteins from this organism are the subject of intense scientific interest and have been used to derive basic understanding of the functioning similar proteins in higher eukaryotes.Zinc Fingers: Motifs in DNA- and RNA-binding proteins whose amino acids are folded into a single structural unit around a zinc atom. In the classic zinc finger, one zinc atom is bound to two cysteines and two histidines. In between the cysteines and histidines are 12 residues which form a DNA binding fingertip. By variations in the composition of the sequences in the fingertip and the number and spacing of tandem repeats of the motif, zinc fingers can form a large number of different sequence specific binding sites.Gene Expression Regulation: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.Germ Cells: The reproductive cells in multicellular organisms at various stages during GAMETOGENESIS.Stem Cells: Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.Drosophila Proteins: Proteins that originate from insect species belonging to the genus DROSOPHILA. The proteins from the most intensely studied species of Drosophila, DROSOPHILA MELANOGASTER, are the subject of much interest in the area of MORPHOGENESIS and development.Drosophila: A genus of small, two-winged flies containing approximately 900 described species. These organisms are the most extensively studied of all genera from the standpoint of genetics and cytology.Silencer Elements, Transcriptional: Nucleic acid sequences that are involved in the negative regulation of GENETIC TRANSCRIPTION by chromatin silencing.Drosophila melanogaster: A species of fruit fly much used in genetics because of the large size of its chromosomes.
... and Botticelli in the Making of a Physician-Scientist," in David A. Schwartz, ed., Medicine, Science and Dreams: The Making of ... In 2017, Anirban Paul and Josh Huang discovered that the biological identity of neurons are encoded in their transcriptional ... In 1990, work was completed on the Arnold and Mabel Beckman Laboratory, and the Marks Neuroscience Building was opened in 1999 ... Carnegie Institution scientists at Cold Spring Harbor made many contributions to genetics and medicine. In 1908 George H. Shull ...
Created from Archived 17 October 2016 at the Wayback Machine *^ a b Burge S, Parkinson GN, Hazel P, Todd AK, Neidle S (2006). " ... Myers LC, Kornberg RD (2000). "Mediator of transcriptional regulation". Annual Review of Biochemistry. 69: 729-49. doi:10.1146/ ... DNA Building Blocks Can Be Made in Space". NASA. Archived from the original on 23 June 2015. Retrieved 10 August 2011.. ... "DNA Building Blocks Can Be Made in Space, NASA Evidence Suggests". ScienceDaily. Archived from the original on 5 September 2011 ...
The fas is an operon made of six genes (orf 1-6) and a regulatory gene, fasR. Because deletions of some fas genes give a non- ... Gene attR is a transcriptional factor including a helix-turn-helix motif. Its transcription is regulated by the same factors ... Operon vic is an operon made of five genes, located on the bacterial chromosome. The only known gene is vicA, the fourth gene ... The compound cytokin oxidase(orf4) can also create adenine with a reactive nitrogen in position 6, which can react with other ...
For instance, association of Steroid Binding Domain can create a transcriptional switch that can change the expression of a ... When the new allele is created, a heterozygote containing the newly created allele as well as the original will express the new ... In vivo mechanisms like transcriptional switches can create conditional mutations. ... Some mutations alter a gene's DNA base sequence but do not change the function of the protein made by the gene. One study on ...
New constructs and expression of proteins • Sequences and topology. 38: 129-36. doi:10.1016/j.sbi.2016.06.005. PMID 27322762.. ... Brinkrolf K, Schröder J, Pühler A, Tauch A (September 2010). "The transcriptional regulatory repertoire of Corynebacterium ... New constructs and expression of proteins / Sequences and topology. 26: 39-43. doi:10.1016/j.sbi.2014.03.006. PMC 4766836. PMID ... In biology, to make too many copies of a protein or other substance. Overexpression of certain proteins or other substances may ...
... is the process of making inferences and predictions about biological networks. In a topological ... Many types of biological networks exist, including transcriptional, signalling and metabolic. Few such networks are known in ... the scientist typically attempts to reconstruct the transcriptional regulatory network. This can be done by data integration in ... "Large-scale mapping and validation of Escherichia coli transcriptional regulation from a compendium of expression profiles". ...
Bound PER completely stops the transcriptional activity of CLK and CYC. In the early morning, the appearance of light causes ... They discovered that mutations in the X chromosome of D. melanogaster could make abnormal circadian activities. When a specific ... but the incoming of PDP1 creates a competition by activating Clk. PER/TIM dimer accumulate in the early night and translocate ... PER and TIM proteins to breakdown in a network of transcriptional activation and repression. First, light activates the cry ...
Veitia RA (November 2008). "One thousand and one ways of making functionally similar transcriptional enhancers". BioEssays. 30 ... With a carefully constructed standard curve, qPCR can produce an absolute measurement of the number of copies of original mRNA ... The ribosome helps transfer RNA to bind to messenger RNA and takes the amino acid from each transfer RNA and makes a structure- ... However, transcriptional silencing may be of more importance than mutation in causing progression to cancer. For example, in ...
The human ATF5 protein is made up of 282 amino acids. ATF5 is a transcription factor that contains a bZip domain. Activating ... ATF5 expression is regulated at both the transcriptional and translational level. ATF5 is expressed in VZ and SVZ during brain ...
His5 then makes L-histidinol-phosphate, which is then hydrolyzed by His2 making histidinol. His4 catalyzes the oxidation of L- ... MetR transcriptional activity is regulated by homocystein, which is the metabolic precursor of methionine. It is also known ... Tyrosine can also be inhibited at the transcriptional level by the TyrR repressor. TyrR binds to the TyrR boxes on the operon ... OAS is a precursor of NAS, cysteine itself can inhibit CysE which functions to create OAS. Without the necessary OAS, NAS will ...
The structure of a transcriptional factory directly relates to its function. Transcription is made more efficient because of ... Experimentally, it appears that they remain fixed over a short period of time; newly made mRNA were pulse labelled over 15 ... The structure of a transcription factory appears to be determined by cell type, transcriptional activity of the cell and also ... This requirement will attract the gene loci to the factory thus creating a loop. The second mechanism suggests that chromatin ...
Often, annotations make book to a specific part of the new data. Textual scholarship is a discipline that often uses the ... In linguistics, annotations include comments and metadata; these non-transcriptional annotations are also non-linguistic. A ... Once a genome is sequenced, it needs to be annotated to make sense of it. In the digital imaging community the term annotation ... It is possible to create meta-annotations out of the existing ones in Java. Since the 1980s, molecular biology and ...
The first way is post translational modification of the amino acids that make up histone proteins. Histone proteins are made up ... When this occurs, complexes like SWI/SNF and other transcriptional factors can bind to the DNA and allow transcription to occur ... are used to build comprehensive databases.[73] sRNA-gene maps based on their targets in microbial genomes are also constructed. ... These newly constructed networks function very efficiently via new pathways as soon as drugs of abuse are further taken ... In ...
It is widely hypothesized that Atrophin-1 functions as a transcriptional co-repressor. A transcriptional co-repressor is a ... It is made up of cytosine, adenine, and guanine. The number of CAG repeats in the ATN1 gene in a healthy person will range from ... There is evidence provided by studies of Atrophin-1 in animals to suggest it acts as a transcriptional co-repressor. Atrophin-1 ...
For instance, association of Steroid Binding Domain can create a transcriptional switch that can change the expression of a ... When the new allele is created, a heterozygote containing the newly created allele as well as the original will express the new ... In vivo mechanisms like transcriptional switches can create conditional mutations. ... Mayr, Ernst (2007). What Makes Biology Unique?: Considerations on the Autonomy of a Scientific Discipline. Cambridge University ...
... is created by the dimerization of two specific alpha helix monomers bound to DNA. The bZIP interacts with the ... The mechanism of transcriptional regulation by bZIP proteins has been studied in detail. Most bZIP proteins show high binding ... Leucine zipper domains are considered a subtype of coiled coils, which are built by two or more alpha helices that are wound ... Instead, these bZIP proteins form heterodimers with other bZIPs to regulate transcriptional activities. ...
From these studies, inferences on the function of histone modifications were made; that transcriptional activation or ... The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high- ... The degree to which chromatin is condensed is associated with a certain transcriptional state. Unpackaged or loose chromatin is ... The logic behind performing epigenetic analysis on a global level is that inferences can be made about epigenetic modifications ...
The VP64-p65-Rta, or VPR, dCas9 activator was created by modifying an existing dCas9 activator, in which a Vp64 transcriptional ... dCas9 constructs have been made that turn on a dCas9-activator fusion protein in the presence of light or chemicals. Cells can ... In order for a protein to be made from the gene that encodes it, RNA polymerase must make RNA from the DNA template of the gene ... See: Transcriptional Activator, Transcription Factor Transcriptional Activators are protein domains or whole proteins linked to ...
shRNA constructs typically encode sense and antisense sequences of 20 - 30 nucleotides. Flexibility in construct design is ... followed by a transcriptional terminator. The antisense species processed from the shRNA can bind to the target RNA and specify ... This construct was designed to express three therapeutic RNAs, one of which was a shRNA, thereby combating HIV replication in ... Multi-cassette constructs that target multiple sites within the same viral RNA circumvent this issue. Delivery of ddRNAi DNA ...
For example, a research team has actually constructed a PAC shuttle vector that creates a library representing two-fold ... Moreover, these studies can serve as a powerful way to investigate transcriptional regulation as it has been seen in the study ... This creates fragments that are similar in size, each containing one or more genes. Insert the fragments of DNA into vectors ... In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme ...
"Potent cancer drugs made -- Sea squirts provide recipe".. *^ a b c Rath CM, et al. (November 2011). "Meta-omic characterization ... Other than transcriptional interference, the mechanism of action of Trabectedin is complex and not completely understood. The ... The EtuO and EtuF3 enzymes continue to post-translationally modify the molecule, adding several functional groups and making a ... By reversing the genetic program created by this transcription factor, Trabectedin promotes differentiation and reverses the ...
It contains an ISWI ATPase, making it part of the ISWI family of chromatin remodeling complexes. NURF is highly conserved among ... eukaryotes and is involved in transcriptional regulation of developmental genes. NURF was first purified from the model ...
2006). "A regulatory SNP causes a human genetic disease by creating a new transcriptional promoter". Science. 312 (5777): 1215- ...
In general, a targeting construct made out of DNA is generated in bacteria. It typically contains part of the gene to be ... However, it can be used for any gene, regardless of transcriptional activity or gene size. Gene targeting methods are ... This makes gene trapping more easily amenable for large scale projects than targeting. On the other hand, gene targeting can be ... To target genes in mice, this construct is then inserted into mouse embryonic stem cells in culture. After cells with the ...
... or transcriptional mediators/intermediary factor 2 (TIF2). NCoA-2 is a transcriptional coregulatory protein that contains ... NCOA2 in turn acetylates histones, which makes downstream DNA more accessible to transcription. Hence, NCOA2 assists nuclear ... GRIP1 is a transcriptional co-activator of the glucocorticoid receptor and interferon regulatory factor 1 (IRF1). Nuclear ... Ishitani K, Yoshida T, Kitagawa H, Ohta H, Nozawa S, Kato S (2003). "p54nrb acts as a transcriptional coactivator for ...
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding. • RNA ...

No FAQ available that match "constructed a transcriptional"