A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It is found in hypertrophic CHONDROCYTES where it may play a role in CHONDROGENESIS and endochondral ossification.
Proteins that are coded by immediate-early genes, in the absence of de novo protein synthesis. The term was originally used exclusively for viral regulatory proteins that were synthesized just after viral integration into the host cell. It is also used to describe cellular proteins which are synthesized immediately after the resting cell is stimulated by extracellular signals.
Regulatory proteins and peptides that are signaling molecules involved in the process of PARACRINE COMMUNICATION. They are generally considered factors that are expressed by one cell and are responded to by receptors on another nearby cell. They are distinguished from HORMONES in that their actions are local rather than distal.
Tissue that supports and binds other tissues. It consists of CONNECTIVE TISSUE CELLS embedded in a large amount of EXTRACELLULAR MATRIX.
A CCN protein family member found at high levels in NEPHROBLASTOMA cells. It is found both intracellularly and in the EXTRACELLULAR MATRIX and may play a role in the regulation of CELL PROLIFERATION and EXTRACELLULAR MATRIX synthesis.
Signal molecules that are involved in the control of cell growth and differentiation.
A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.
Any pathological condition where fibrous connective tissue invades any organ, usually as a consequence of inflammation or other injury.
A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.
A heterogeneous group of disorders, some hereditary, others acquired, characterized by abnormal structure or function of one or more of the elements of connective tissue, i.e., collagen, elastin, or the mucopolysaccharides.
A CCN protein family member that regulates a variety of extracellular functions including CELL ADHESION; CELL MIGRATION; and EXTRACELLULAR MATRIX synthesis. It may play an important role in the development of branched CAPILLARIES during EMBRYOGENESIS.
A group of cells that includes FIBROBLASTS, cartilage cells, ADIPOCYTES, smooth muscle cells, and bone cells.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
A family of secreted proteins found associated with the EXTRACELLULAR MATRIX and cell surface receptors. They are believed to play a role in modulating the effects of a variety of GROWTH FACTORS and PROTEASES at the cell membrane extracellular matrix. The CCN protein family is named after three protypical members; CYSTEINE-RICH PROTEIN 61; CONNECTIVE TISSUE GROWTH FACTOR; and NEPHROBLASTOMA OVEREXPRESSED PROTEIN.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The most common form of fibrillar collagen. It is a major constituent of bone (BONE AND BONES) and SKIN and consists of a heterotrimer of two alpha1(I) and one alpha2(I) chains.
A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).
A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
A chronic multi-system disorder of CONNECTIVE TISSUE. It is characterized by SCLEROSIS in the SKIN, the LUNGS, the HEART, the GASTROINTESTINAL TRACT, the KIDNEYS, and the MUSCULOSKELETAL SYSTEM. Other important features include diseased small BLOOD VESSELS and AUTOANTIBODIES. The disorder is named for its most prominent feature (hard skin), and classified into subsets by the extent of skin thickening: LIMITED SCLERODERMA and DIFFUSE SCLERODERMA.
A syndrome with overlapping clinical features of systemic lupus erythematosus, scleroderma, polymyositis, and Raynaud's phenomenon. The disease is differentially characterized by high serum titers of antibodies to ribonuclease-sensitive extractable (saline soluble) nuclear antigen and a "speckled" epidermal nuclear staining pattern on direct immunofluorescence.
Glycoproteins found on the surfaces of cells, particularly in fibrillar structures. The proteins are lost or reduced when these cells undergo viral or chemical transformation. They are highly susceptible to proteolysis and are substrates for activated blood coagulation factor VIII. The forms present in plasma are called cold-insoluble globulins.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Fibroblasts which occur in the CORNEAL STROMA.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
A receptor-regulated smad protein that undergoes PHOSPHORYLATION by ACTIVIN RECEPTORS, TYPE I. It regulates TRANSFORMING GROWTH FACTOR BETA and ACTIVIN signaling.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
A plant genus of the family SAURURACEAE. Members contain aristolactams.
Macromolecular organic compounds that contain carbon, hydrogen, oxygen, nitrogen, and usually, sulfur. These macromolecules (proteins) form an intricate meshwork in which cells are embedded to construct tissues. Variations in the relative types of macromolecules and their organization determine the type of extracellular matrix, each adapted to the functional requirements of the tissue. The two main classes of macromolecules that form the extracellular matrix are: glycosaminoglycans, usually linked to proteins (proteoglycans), and fibrous proteins (e.g., COLLAGEN; ELASTIN; FIBRONECTINS; and LAMININ).
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Smooth muscle-like cells adhering to the wall of the small blood vessels of the KIDNEY at the glomerulus and along the vascular pole of the glomerulus in the JUXTAGLOMERULAR APPARATUS. They are myofibroblasts with contractile and phagocytic properties. These cells and their MESANGIAL EXTRACELLULAR MATRIX constitute the GLOMERULAR MESANGIUM.
A TGF-beta subtype that was originally identified as a GLIOBLASTOMA-derived factor which inhibits the antigen-dependent growth of both helper and CYTOTOXIC T LYMPHOCYTES. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta2 and TGF-beta2 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor.
A 6-kDa polypeptide growth factor initially discovered in mouse submaxillary glands. Human epidermal growth factor was originally isolated from urine based on its ability to inhibit gastric secretion and called urogastrone. Epidermal growth factor exerts a wide variety of biological effects including the promotion of proliferation and differentiation of mesenchymal and EPITHELIAL CELLS. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
A family of proteins that are involved in the translocation of signals from TGF-BETA RECEPTORS; BONE MORPHOGENETIC PROTEIN RECEPTORS; and other surface receptors to the CELL NUCLEUS. They were originally identified as a class of proteins that are related to the mothers against decapentaplegic protein, Drosophila and sma proteins from CAENORHABDITIS ELEGANS.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
The thin membranous structure supporting the adjoining glomerular capillaries. It is composed of GLOMERULAR MESANGIAL CELLS and their EXTRACELLULAR MATRIX.
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
A fibrillar collagen consisting of three identical alpha1(III) chains that is widely distributed in many tissues containing COLLAGEN TYPE I. It is particularly abundant in BLOOD VESSELS and may play a role in tissues with elastic characteristics.
A receptor-regulated smad protein that undergoes PHOSPHORYLATION by ACTIVIN RECEPTORS, TYPE I. Activated Smad3 can bind directly to DNA, and it regulates TRANSFORMING GROWTH FACTOR BETA and ACTIVIN signaling.
Cell-surface proteins that bind transforming growth factor beta and trigger changes influencing the behavior of cells. Two types of transforming growth factor receptors have been recognized. They differ in affinity for different members of the transforming growth factor beta family and in cellular mechanisms of action.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed)
Restoration of integrity to traumatized tissue.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Established cell cultures that have the potential to propagate indefinitely.
A process in which normal lung tissues are progressively replaced by FIBROBLASTS and COLLAGEN causing an irreversible loss of the ability to transfer oxygen into the bloodstream via PULMONARY ALVEOLI. Patients show progressive DYSPNEA finally resulting in death.
A cell surface receptor involved in regulation of cell growth and differentiation. It is specific for EPIDERMAL GROWTH FACTOR and EGF-related peptides including TRANSFORMING GROWTH FACTOR ALPHA; AMPHIREGULIN; and HEPARIN-BINDING EGF-LIKE GROWTH FACTOR. The binding of ligand to the receptor causes activation of its intrinsic tyrosine kinase activity and rapid internalization of the receptor-ligand complex into the cell.
A family of soluble proteins that bind insulin-like growth factors and modulate their biological actions at the cellular level. (Int J Gynaecol Obstet 1992;39(1):3-9)
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
A non-fibrillar collagen found in the structure of BASEMENT MEMBRANE. Collagen type IV molecules assemble to form a sheet-like network which is involved in maintaining the structural integrity of basement membranes. The predominant form of the protein is comprised of two alpha1(IV) subunits and one alpha2(IV) subunit, however, at least six different alpha subunits can be incorporated into the heterotrimer.
Perisinusoidal cells of the liver, located in the space of Disse between HEPATOCYTES and sinusoidal endothelial cells.
KIDNEY injuries associated with diabetes mellitus and affecting KIDNEY GLOMERULUS; ARTERIOLES; KIDNEY TUBULES; and the interstitium. Clinical signs include persistent PROTEINURIA, from microalbuminuria progressing to ALBUMINURIA of greater than 300 mg/24 h, leading to reduced GLOMERULAR FILTRATION RATE and END-STAGE RENAL DISEASE.
Diabetes mellitus induced experimentally by administration of various diabetogenic agents or by PANCREATECTOMY.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Proteins prepared by recombinant DNA technology.
A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Hyperplasia of the mucous membrane of the lips, tongue, and less commonly, the buccal mucosa, floor of the mouth, and palate, presenting soft, painless, round to oval sessile papules about 1 to 4 mm in diameter. The condition usually occurs in children and young adults and has familial predilection, lasting for several months, sometimes years, before running its course. A viral etiology is suspected, the isolated organism being usually the human papillomavirus. (Jablonski, Illustrated Dictionary of Dentistry; Belshe, Textbook of Human Virology, 2d ed, p954)
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A single-chain polypeptide growth factor that plays a significant role in the process of WOUND HEALING and is a potent inducer of PHYSIOLOGIC ANGIOGENESIS. Several different forms of the human protein exist ranging from 18-24 kDa in size due to the use of alternative start sites within the fgf-2 gene. It has a 55 percent amino acid residue identity to FIBROBLAST GROWTH FACTOR 1 and has potent heparin-binding activity. The growth factor is an extremely potent inducer of DNA synthesis in a variety of cell types from mesoderm and neuroectoderm lineages. It was originally named basic fibroblast growth factor based upon its chemical properties and to distinguish it from acidic fibroblast growth factor (FIBROBLAST GROWTH FACTOR 1).
Generalized or localized diffuse fibrous overgrowth of the gingival tissue, usually transmitted as an autosomal dominant trait, but some cases are idiopathic and others produced by drugs. The enlarged gingiva is pink, firm, and has a leather-like consistency with a minutely pebbled surface and in severe cases the teeth are almost completely covered and the enlargement projects into the oral vestibule. (Dorland, 28th ed)
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Spindle-shaped cells with characteristic CONTRACTILE PROTEINS and structures that contribute to the WOUND HEALING process. They occur in GRANULATION TISSUE and also in pathological processes such as FIBROSIS.
A 3.5 per cent colloidal solution containing urea-cross-linked polymerized peptides. It has a molecular weight of approximately 35,000 and is prepared from gelatin and electrolytes. The polymeric solution is used as a plasma expander.
A layer of vascularized connective tissue underneath the EPIDERMIS. The surface of the dermis contains innervated papillae. Embedded in or beneath the dermis are SWEAT GLANDS; HAIR FOLLICLES; and SEBACEOUS GLANDS.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on GROWTH HORMONE. It is believed to be mainly active in adults in contrast to INSULIN-LIKE GROWTH FACTOR II, which is a major fetal growth factor.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Long convoluted tubules in the nephrons. They collect filtrate from blood passing through the KIDNEY GLOMERULUS and process this filtrate into URINE. Each renal tubule consists of a BOWMAN CAPSULE; PROXIMAL KIDNEY TUBULE; LOOP OF HENLE; DISTAL KIDNEY TUBULE; and KIDNEY COLLECTING DUCT leading to the central cavity of the kidney (KIDNEY PELVIS) that connects to the URETER.
Multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. Its receptor is PROTO-ONCOGENE PROTEINS C-MET.
Mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.
Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
Polymorphic cells that form cartilage.
A RHO GTP-BINDING PROTEIN involved in regulating signal transduction pathways that control assembly of focal adhesions and actin stress fibers. This enzyme was formerly listed as EC 3.6.1.47.
Elements of limited time intervals, contributing to particular results or situations.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
A family of small polypeptide growth factors that share several common features including a strong affinity for HEPARIN, and a central barrel-shaped core region of 140 amino acids that is highly homologous between family members. Although originally studied as proteins that stimulate the growth of fibroblasts this distinction is no longer a requirement for membership in the fibroblast growth factor family.
Unique slender cells with multiple processes extending along the capillary vessel axis and encircling the vascular wall, also called mural cells. Pericytes are imbedded in the BASEMENT MEMBRANE shared with the ENDOTHELIAL CELLS of the vessel. Pericytes are important in maintaining vessel integrity, angiogenesis, and vascular remodeling.
Hormonally active polypeptides that can induce the transformed phenotype when added to normal, non-transformed cells. They have been found in culture fluids from retrovirally transformed cells and in tumor-derived cells as well as in non-neoplastic sources. Their transforming activities are due to the simultaneous action of two otherwise unrelated factors, TRANSFORMING GROWTH FACTOR ALPHA and TRANSFORMING GROWTH FACTOR BETA.
Laboratory mice that have been produced from a genetically manipulated EGG or EMBRYO, MAMMALIAN.
An intracellular signaling system involving the MAP kinase cascades (three-membered protein kinase cascades). Various upstream activators, which act in response to extracellular stimuli, trigger the cascades by activating the first member of a cascade, MAP KINASE KINASE KINASES; (MAPKKKs). Activated MAPKKKs phosphorylate MITOGEN-ACTIVATED PROTEIN KINASE KINASES which in turn phosphorylate the MITOGEN-ACTIVATED PROTEIN KINASES; (MAPKs). The MAPKs then act on various downstream targets to affect gene expression. In mammals, there are several distinct MAP kinase pathways including the ERK (extracellular signal-regulated kinase) pathway, the SAPK/JNK (stress-activated protein kinase/c-jun kinase) pathway, and the p38 kinase pathway. There is some sharing of components among the pathways depending on which stimulus originates activation of the cascade.
Short fragments of DNA or RNA that are used to alter the function of target RNAs or DNAs to which they hybridize.
The development of new BLOOD VESSELS during the restoration of BLOOD CIRCULATION during the healing process.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
Derivatives of PHOSPHATIDIC ACIDS that lack one of its fatty acyl chains due to its hydrolytic removal.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
Glycoproteins which have a very high polysaccharide content.
An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
A porelike structure surrounding the entire circumference of the anterior chamber through which aqueous humor circulates to the canal of Schlemm.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A surgical procedure used in treatment of glaucoma in which an opening is created through which aqueous fluid may pass from the anterior chamber into a sac created beneath the conjunctiva, thus lowering the pressure within the eye. (Hoffman, Pocket Glossary of Ophthalmologic Terminology, 1989)
A complex of related glycopeptide antibiotics from Streptomyces verticillus consisting of bleomycin A2 and B2. It inhibits DNA metabolism and is used as an antineoplastic, especially for solid tumors.
Adherence of cells to surfaces or to other cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
An octapeptide that is a potent but labile vasoconstrictor. It is produced from angiotensin I after the removal of two amino acids at the C-terminal by ANGIOTENSIN CONVERTING ENZYME. The amino acid in position 5 varies in different species. To block VASOCONSTRICTION and HYPERTENSION effect of angiotensin II, patients are often treated with ACE INHIBITORS or with ANGIOTENSIN II TYPE 1 RECEPTOR BLOCKERS.
These growth factors are soluble mitogens secreted by a variety of organs. The factors are a mixture of two single chain polypeptides which have affinity to heparin. Their molecular weight are organ and species dependent. They have mitogenic and chemotactic effects and can stimulate endothelial cells to grow and synthesize DNA. The factors are related to both the basic and acidic FIBROBLAST GROWTH FACTORS but have different amino acid sequences.
An extracellular matrix glycoprotein from platelets and a variety of normal and transformed cells of both mesenchymal and epithelial origin. Thrombospondin-1 is believed to play a role in cell migration and proliferation, during embryogenesis and wound repair. Also, it has been studied for its use as a potential regulator of tumor growth and metastasis.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Either of the pair of organs occupying the cavity of the thorax that effect the aeration of the blood.
Inflammation of the interstitial tissue of the kidney. This term is generally used for primary inflammation of KIDNEY TUBULES and/or surrounding interstitium. For primary inflammation of glomerular interstitium, see GLOMERULONEPHRITIS. Infiltration of the inflammatory cells into the interstitial compartment results in EDEMA, increased spaces between the tubules, and tubular renal dysfunction.
A cell surface receptor mediating cell adhesion to the EXTRACELLULAR MATRIX and to other cells via binding to LAMININ. It is involved in cell migration, embryonic development, leukocyte activation and tumor cell invasiveness. Integrin alpha6beta1 is the major laminin receptor on PLATELETS; LEUKOCYTES; and many EPITHELIAL CELLS, and ligand binding may activate a number of signal transduction pathways. Alternative splicing of the cytoplasmic domain of the alpha6 subunit (INTEGRIN ALPHA6) results in the formation of A and B isoforms of the heterodimer, which are expressed in a tissue-specific manner.
A mitogen-activated protein kinase subfamily that is widely expressed and plays a role in regulation of MEIOSIS; MITOSIS; and post mitotic functions in differentiated cells. The extracellular signal regulated MAP kinases are regulated by a broad variety of CELL SURFACE RECEPTORS and can be activated by certain CARCINOGENS.
An EPIDERMAL GROWTH FACTOR related protein that is found in a variety of tissues including EPITHELIUM, and maternal DECIDUA. It is synthesized as a transmembrane protein which can be cleaved to release a soluble active form which binds to the EGF RECEPTOR.
A receptor-regulated smad protein that undergoes PHOSPHORYLATION by BONE MORPHOGENETIC PROTEIN RECEPTORS. It regulates BONE MORPHOGENETIC PROTEIN signaling and plays an essential role in EMBRYONIC DEVELOPMENT.
The renal tubule portion that extends from the BOWMAN CAPSULE in the KIDNEY CORTEX into the KIDNEY MEDULLA. The proximal tubule consists of a convoluted proximal segment in the cortex, and a distal straight segment descending into the medulla where it forms the U-shaped LOOP OF HENLE.
A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.
Blockage in any part of the URETER causing obstruction of urine flow from the kidney to the URINARY BLADDER. The obstruction may be congenital, acquired, unilateral, bilateral, complete, partial, acute, or chronic. Depending on the degree and duration of the obstruction, clinical features vary greatly such as HYDRONEPHROSIS and obstructive nephropathy.
A 44-kDa extracellular signal-regulated MAP kinase that may play a role the initiation and regulation of MEIOSIS; MITOSIS; and postmitotic functions in differentiated cells. It phosphorylates a number of TRANSCRIPTION FACTORS; and MICROTUBULE-ASSOCIATED PROTEINS.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
Pathological processes of the KIDNEY or its component tissues.
Methods for maintaining or growing CELLS in vitro.
A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Liver disease in which the normal microcirculation, the gross vascular anatomy, and the hepatic architecture have been variably destroyed and altered with fibrous septa surrounding regenerated or regenerating parenchymal nodules.
Factors which enhance the growth potentialities of sensory and sympathetic nerve cells.
A family of angiogenic proteins that are closely-related to VASCULAR ENDOTHELIAL GROWTH FACTOR A. They play an important role in the growth and differentiation of vascular as well as lymphatic endothelial cells.
A cell line derived from cultured tumor cells.
A proline-directed serine/threonine protein kinase which mediates signal transduction from the cell surface to the nucleus. Activation of the enzyme by phosphorylation leads to its translocation into the nucleus where it acts upon specific transcription factors. p40 MAPK and p41 MAPK are isoforms.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
A non-vascular form of connective tissue composed of CHONDROCYTES embedded in a matrix that includes CHONDROITIN SULFATE and various types of FIBRILLAR COLLAGEN. There are three major types: HYALINE CARTILAGE; FIBROCARTILAGE; and ELASTIC CARTILAGE.
Refers to animals in the period of time just after birth.
A hydroxylated form of the imino acid proline. A deficiency in ASCORBIC ACID can result in impaired hydroxyproline formation.
The relationship between the dose of an administered drug and the response of the organism to the drug.
Bone-forming cells which secrete an EXTRACELLULAR MATRIX. HYDROXYAPATITE crystals are then deposited into the matrix to form bone.
A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)

Expression and differential regulation of connective tissue growth factor in pancreatic cancer cells. (1/856)

CTGF is an immediate early growth responsive gene that has been shown to be a downstream mediator of TGFbeta actions in fibroblasts and vascular endothelial cells. In the present study hCTGF was isolated as immediate early target gene of EGF/TGFalpha in human pancreatic cancer cells by suppression hybridization. CTGF transcripts were found in 13/15 pancreatic cancer cell lines incubated with 10% serum. In 3/7 pancreatic cancer cell lines EGF/TGFalpha induced a significant rise of CTGF transcript levels peaking 1-2 h after the start of treatment. TGFbeta increased CTGF transcript levels in 2/7 pancreatic cancer cell lines after 4 h of treatment and this elevation was sustained after 24 h. Only treatment with TGFbeta was accompanied by a parallel induction of collagen type I transcription. 15/19 human pancreatic cancer tissues were shown to overexpress high levels of CTGF transcripts. CTGF transcript levels in pancreatic cancer tissues and nude mouse xenograft tumors showed a good correlation to the degree of fibrosis. In situ hybridization and the nude mouse experiments revealed that in pancreatic cancer tissues, fibroblasts are the predominant site of CTGF transcription, whereas the tumor cells appear to contribute to a lesser extent. We conclude that CTGF may be of paramount importance for the development of the characteristic desmoplastic reaction in pancreatic cancer tissues.  (+info)

Suppression subtractive hybridization identifies high glucose levels as a stimulus for expression of connective tissue growth factor and other genes in human mesangial cells. (2/856)

Accumulation of mesangial matrix is a pivotal event in the pathophysiology of diabetic nephropathy. The molecular triggers for matrix production are still being defined. Here, suppression subtractive hybridization identified 15 genes differentially induced when primary human mesangial cells are exposed to high glucose (30 mM versus 5 mM) in vitro. These genes included (a) known regulators of mesangial cell activation in diabetic nephropathy (fibronectin, caldesmon, thrombospondin, and plasminogen activator inhibitor-1), (b) novel genes, and (c) known genes whose induction by high glucose has not been reported. Prominent among the latter were genes encoding cytoskeleton-associated proteins and connective tissue growth factor (CTGF), a modulator of fibroblast matrix production. In parallel experiments, elevated CTGF mRNA levels were demonstrated in glomeruli of rats with streptozotocin-induced diabetic nephropathy. Mannitol provoked less mesangial cell CTGF expression in vitro than high glucose, excluding hyperosmolality as the key stimulus. The addition of recombinant CTGF to cultured mesangial cells enhanced expression of extracellular matrix proteins. High glucose stimulated expression of transforming growth factor beta1 (TGF-beta1), and addition of TGF-beta1 to mesangial cells triggered CTGF expression. CTGF expression induced by high glucose was partially suppressed by anti-TGF-beta1 antibody and by the protein kinase C inhibitor GF 109203X. Together, these data suggest that 1) high glucose stimulates mesangial CTGF expression by TGFbeta1-dependent and protein kinase C dependent pathways, and 2) CTGF may be a mediator of TGFbeta1-driven matrix production within a diabetic milieu.  (+info)

Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion and migration through integrin alphavbeta3, promotes endothelial cell survival, and induces angiogenesis in vivo. (3/856)

Fisp12 was first identified as a secreted protein encoded by a growth factor-inducible immediate-early gene in mouse fibroblasts, whereas its human ortholog, CTGF (connective tissue growth factor), was identified as a mitogenic activity in conditioned media of human umbilical vein endothelial cells. Fisp12/CTGF is a member of a family of secreted proteins that includes CYR61, Nov, Elm-1, Cop-1/WISP-2, and WISP-3. Fisp12/CTGF has been shown to promote cell adhesion and mitogenesis in both fibroblasts and endothelial cells and to stimulate cell migration in fibroblasts. These findings, together with the localization of Fisp12/CTGF in angiogenic tissues, as well as in atherosclerotic plaques, suggest a possible role for Fisp12/CTGF in the regulation of vessel growth during development, wound healing, and vascular disease. In this study, we show that purified Fisp12 (mCTGF) protein promotes the adhesion of microvascular endothelial cells through the integrin receptor alphavbeta3. Furthermore, Fisp12 stimulates the migration of microvascular endothelial cells in culture, also through an integrin-alphavbeta3-dependent mechanism. In addition, the presence of Fisp12 promotes endothelial cell survival when cells are plated on laminin and deprived of growth factors, a condition that otherwise induces apoptosis. In vivo, Fisp12 induces neovascularization in rat corneal micropocket implants. These results demonstrate that Fisp12 is a novel angiogenic inducer and suggest a direct role for Fisp12 in the adhesion, migration, and survival of endothelial cells during blood vessel growth. Taken together with the recent finding that the related protein CYR61 also induces angiogenesis, we suggest that Fisp12/mCTGF and CYR61 comprise prototypes of a new family of angiogenic regulators that function, at least in part, through integrin-alphavbeta3-dependent pathways.  (+info)

Involvement of cis-acting repressive element(s) in the 3'-untranslated region of human connective tissue growth factor gene. (4/856)

To analyze the regulatory mechanism of connective tissue growth factor expression, the 3'-untranslated region (3'-UTR) of CTGF cDNA was amplified from HeLa cell RNA. Direct nucleotide sequencing revealed a single major population in the amplicon, which was nearly identical to other sequences. Subsequently, the effect of the 3'-UTR on gene expression was evaluated. When it was fused downstream of a firefly luciferase gene, the 3'-UTR strongly repressed luciferase gene expression. Interestingly, the repressive effect of the antisense 3'-UTR appeared to be more prominent than that of the sense one. Together with the fact that several consensus sequences for regulatory elements are found in it, these results suggest the involvement of multiple sets of regulatory elements in the CTGF 3'-UTR.  (+info)

Connective tissue growth factor induces the proliferation, migration, and tube formation of vascular endothelial cells in vitro, and angiogenesis in vivo. (5/856)

Connective tissue growth factor (CTGF) is a novel cysteine-rich, secreted protein. Recently, we found that inhibition of the endogenous expression of CTGF by its antisense oligonucleotide and antisense RNA suppresses the proliferation and migration of vascular endothelial cells. In the present study, the following observations demonstrated the angiogenic function of CTGF in vitro and in vivo: (i) purified recombinant CTGF (rCTGF) promoted the adhesion, proliferation and migration of vascular endothelial cells in a dose-dependent manner under serum-free conditions, and these effects were inhibited by anti-CTGF antibodies; (ii) rCTGF markedly induced the tube formation of vascular endothelial cells, and this effect was stronger than that of basic fibroblast growth factor or vascular endothelial growth factor; (iii) application of rCTGF to the chicken chorioallantoic membrane resulted in a gross angiogenic response, and this effect was also inhibited by anti-CTGF antibodies. (iv) rCTGF injected with collagen gel into the backs of mice induced strong angiogenesis in vivo. These findings indicate that CTGF is a novel, potent angiogenesis factor which functions in multi-stages in this process.  (+info)

Connective tissue growth factor is a regulator for fibrosis in human chronic pancreatitis. (6/856)

OBJECTIVE: To evaluate the parameters that mediate fibrogenesis in chronic pancreatitis (CP). BACKGROUND: Connective tissue growth factor (CTGF), which is regulated by transforming growth factor beta (TGF-beta), has recently been implicated in skin fibrosis and atherosclerosis. In the present study, the authors analyzed the concomitant presence of TGF-beta1 and its signaling receptors-TGF-beta receptor I, subtype ALK5 (TbetaR-I(ALK5)), and TGF-beta receptor II (TbetaR-II)-as well as CTGF and collagen type I in the pancreatic tissue of patients undergoing surgery for chronic pancreatitis. PATIENTS AND METHODS: CP tissue samples were obtained from 40 patients (8 women, 32 men) undergoing pancreatic resection. Tissue samples of 25 previously healthy organ donors (12 women, 13 men) served as controls. The expression of TGF-beta1, TbetaR-I(ALK5), TbetaR-II, CTGF, and collagen type I was studied by Northern blot analysis. By in situ hybridization and immunohistochemistry, the respective mRNA moieties and proteins were localized in the tissue samples. RESULTS: Northern blot analysis showed that CP tissue samples exhibited concomitant enhanced mRNA expression of TGF-beta1 (38-fold), TbetaR-II (5-fold), CTGF (25-fold), and collagen type I (24-fold) compared with normal controls. In addition, TbetaR-I(ALK5) mRNA was increased in 50% of CP tissue samples (1.8-fold). By in situ hybridization, TGF-beta1, TbetaR-I(ALK5), and TbetaR-II mRNA were often seen to be colocalized, especially in the ductal cells and in metaplastic areas where atrophic acinar cells appeared to dedifferentiate into ductal structures. In contrast, CTGF was located in degenerating acinar cells and principally in fibroblasts surrounding these areas. Moreover, CTGF mRNA expression levels correlated positively with the degree of fibrosis in CP tissues. CONCLUSION: The concomitant overexpression of CTGF, collagen type I, TGF-beta1, and its signaling receptors in CP suggests that these proteins contribute to enhanced extracellular matrix synthesis and accumulation, resulting finally in the fibrogenesis observed in CP.  (+info)

Activation-dependent adhesion of human platelets to Cyr61 and Fisp12/mouse connective tissue growth factor is mediated through integrin alpha(IIb)beta(3). (7/856)

Cyr61 and connective tissue growth factor (CTGF), members of a newly identified family of extracellular matrix-associated signaling molecules, are found to mediate cell adhesion, promote cell migration and enhance growth factor-induced cell proliferation in vitro, and induce angiogenesis in vivo. We previously showed that vascular endothelial cell adhesion and migration to Cyr61 and Fisp12 (mouse CTGF) are mediated through integrin alpha(v)beta(3). Both Cyr61 and Fisp12/mCTGF are present in normal blood vessel walls, and it has been demonstrated that CTGF is overexpressed in advanced atherosclerotic lesions. In the present study, we examined whether Cyr61 and Fisp12/mCTGF could serve as substrates for platelet adhesion. Agonist (ADP, thrombin, or U46619)-stimulated but not resting platelets adhered to both Cyr61 and Fisp12/mCTGF, and this process was completely inhibited by prostaglandin I(2), which prevents platelet activation. The specificity of Cyr61- and Fisp12/mCTGF-mediated platelet adhesion was demonstrated by specific inhibition of this process with polyclonal anti-Cyr61 and anti-Fisp12/mCTGF antibodies, respectively. The adhesion of ADP-activated platelets to both proteins was divalent cation-dependent and was blocked by RGDS, HHLGGAKQAGDV, or echistatin, but not by RGES. Furthermore, this process was specifically inhibited by the monoclonal antibody AP-2 (anti-alpha(IIb)beta(3)), but not by LM609 (anti-alpha(v)beta(3)), indicating that the interaction is mediated through integrin alpha(IIb)beta(3). In a solid phase binding assay, activated alpha(IIb)beta(3), purified by RGD affinity chromatography, bound to immobilized Cyr61 and Fisp12/mCTGF in a dose-dependent and RGD-inhibitable manner. In contrast, unactivated alpha(IIb)beta(3) failed to bind to either protein. Collectively, these findings identify Cyr61 and Fisp12/mCTGF as two novel activation-dependent adhesive ligands for the integrin alpha(IIb)beta(3) on human platelets, and implicate a functional role for these proteins in hemostasis and thrombosis.  (+info)

Nuclear localisation of NOVH protein: a potential role for NOV in the regulation of gene expression. (8/856)

AIMS: To identify the NOV protein detected by immunofluorescence in the nucleus of human cancer cell lines to establish whether targeting to the nucleus reflects dual paracrine and intracrine biological functions of NOV, as has been reported previously for several signalling peptides and proteins. METHODS: Nuclear and cytoplasmic fractions were prepared from 143 and HeLa cells in which nuclear NOV protein was detected. Western blotting analysis of NOV proteins in both types of fractions was performed using two NOV specific antibodies. Confocal microscopy was used to visualise the nuclear NOV protein in HeLa and 143 cells. A yeast two hybrid screening system was used to isolate cDNAs encoding proteins able to interact with the human NOV protein. RESULTS: A 31/32 kDa doublet of NOV protein was identified in the nuclear fraction of 143 and HeLa cells. Because the antibodies were directed against the C-terminus of NOV, the 31/32 kDa NOV isoform is probably truncated at the N-terminus and might correspond to the secreted 32 kDa NOV isoform detected in cell culture medium. Confocal microscopy indicated that in addition to the cytoplasmic NOV protein already identified, a nuclear NOV protein was present in both the nucleoplasm and nucleoli of Hela and 143 cells. Screening of cDNA libraries prepared from HeLa cells, Epstein-Barr virus transformed lymphocytes, and normal human brain showed that the NOV protein interacts with the rpb7 subunit of RNA polymerase in a yeast two hybrid system. CONCLUSIONS: The NOV protein detected in the nucleus of 143 and HeLa cells is probably an N-terminus truncated isoform of the secreted 48 kDa NOV protein. A growing body of evidence suggests that novH expression is closely associated with differentiation in normal human tissues and that the nov gene encodes a signalling protein that belongs to an emerging family of cell growth regulators. The nuclear localisation of a NOV isoform potentially provides an additional degree of signalling specificity. The interaction of the NOV protein and the rpb7 subunit of RNA polymerase II in the two hybrid system suggests that NOV might be involved in regulating gene expression at the transcriptional level. As has already been suggested for several other nuclearly located cytokines, the NOV protein does not contain a typical nuclear localisation signal. Therefore, it is possible that it combines with either a receptor or a chaperone during its translocation. Disruption of the balance between the secreted and nuclear NOV isoforms might affect the putative autocrine and paracrine functions of NOV and might be of considerable importance in the development of cancers in which the expression of novH has been shown to be impaired.  (+info)

Global Connective Tissue Growth Factor Sales Market Report 2018 1 Connective Tissue Growth Factor Market Overview 1.1 Product Overview and Scope of Connective Tissue Growth Factor 1.2 Classification of Connective Tissue Growth Factor by Product Category 1.2.1 Global Connective Tissue Growth Factor Market Size (Sales) Comparison by Type (2013-2025) 1.2.2 Global Connective Tissue Growth Factor Market Size (Sales) Market Share by Type (Product Category) in 2017 1.2.3 BLR-200 1.2.4 IB-DMD 1.2.5 OLX-201 1.2.6 PBI-4050 1.2.7 Others 1.3 Global Connective Tissue Growth Factor Market by Application/End Users 1.3.1 Global Connective Tissue Growth Factor Sales (Volume) and Market Share Comparison by Application (2013-2025) 1.3.2 Hypertrophic Scars 1.3.3 Opthalmology 1.3.4 Genetic Disorders 1.3.5 Liver Fibrosis 1.3.6 Others 1.4 Global Connective Tissue Growth Factor Market by Region 1.4.1 Global Connective Tissue Growth Factor Market Size (Value) Comparison by Region (2013-2025) 1.4.2 United States ...
Connective tissue growth factor (CCN2) drives fibrogenesis in hepatic stellate cells (HSC). in exosomes however not in cell lysates had been decreased by pre-treatment from the ESI-09 cells using the exosome inhibitor GW4869. ESI-09 Co-culture of miR-214-transfected donor HSC with CCN2 3′-UTR luciferase reporter-transfected receiver HSC led to miR-214- and exosome-dependent rules of a crazy type CCN2 3′-UTR reporter however not of the mutant CCN2 3′-UTR reporter missing the miR-214 binding site. Exosomes from HSC had been a conduit for uptake of miR-214 by major mouse hepatocytes. Down-regulation of CCN2 manifestation by miR-214 also happened in human being LX-2 HSC in keeping with a conserved miR-214 binding site within the human being CCN2 3′-UTR. MiR-214 in LX-2 cells was shuttled via exosomes to receiver LX-2 cells or human being HepG2 hepatocytes leading to suppression of CCN2 3′-UTR activity or manifestation of CCN2 downstream focuses on including αSMA or collagen. Experimental ...
Table 1: Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role for CTGF in Human Diabetic Foot Ulcer Healing
Table 3: Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role for CTGF in Human Diabetic Foot Ulcer Healing
Angiotensin II (Ang II) promotes hepatic fibrosis by increasing extracellular matrix (ECM) synthesis. Connective tissue growth factor (CTGF) plays a crucial role in the pathogenesis of hepatic fibrosis and emerges as downstream of the profibrogenic cytokine transforming growth factor-beta (TGF-beta). We aimed to investigate the molecular events that lead from the Ang II receptor to CTGF upregulation in human hepatic stellate cells, a principal fibrogenic cell type. Ang II produced an early, AT1 receptor-dependent stimulation of CTGF expression and induced a rapid activation of PKC and its downstream p38 MAPK, thereby activating a nuclear factor-kappa B (NF-kappa B) and Smad2/3 cross-talk pathway. Chemical blockade of NF-kappa B and Smad2/3 signaling synergistically diminished Ang II-mediated CTGF induction and exhibited an additive effect in abrogating the ECM accumulation caused by Ang II. Furthermore, we demonstrated that CTGF expression was essential for Ang II-mediated ECM synthesis. ...
TY - JOUR. T1 - Plasma connective tissue growth factor is a novel potential biomarker of cardiac dysfunction in patients with chronic heart failure. AU - Koitabashi, Norimichi. AU - Arai, Masashi. AU - Niwano, Kazuo. AU - Watanabe, Atai. AU - Endoh, Michiko. AU - Suguta, Masahiko. AU - Yokoyama, Tomoyuki. AU - Tada, Hiroshi. AU - Toyama, Takuji. AU - Adachi, Hitoshi. AU - Naito, Shigeto. AU - Oshima, Shigeru. AU - Nishida, Takashi. AU - Kubota, Satoshi. AU - Takigawa, Masaharu. AU - Kurabayashi, Masahiko. PY - 2008/4. Y1 - 2008/4. N2 - Background: Connective tissue growth factor (CTGF) has been recently reported as a mediator of myocardial fibrosis; however, the significance of plasma CTGF concentration has not been evaluated in patients with heart failure. The aim of this study was to investigate the clinical utility of plasma CTGF concentration for the diagnosis of heart failure. Methods and results: We evaluated fifty-two patients with chronic heart failure. The plasma concentration of CTGF ...
Connective tissue growth factor (CTGF/CCN2) is axiomatically necessary for proper skeletal development and function. We need not look further than the studies that have been done to date utilizing mice genetically engineered to lack CTGF production. These CTGF null or knockout (KO) mice fail to form a normal murine skeleton and instead yield one littered with bony dysmorphisms, including incompetent craniofacial development, kinked limb bones, and misshapen ribs that are not conducive to proper respiratory function. As a result, the global lack of CTGF is incompatible with postnatal life. A closer look at several sites demonstrated defects in physiologic processes necessary for bone formation - angiogenesis, chondrogenesis, and osteogenesis. Therefore, the dogma in the CCN protein field to date has been that systemic ablation of CTGF production in vivo results in global defects in bone development. We believe this dogma is an oversimplification of the role of CTGF on skeletal development. Our ...
Connective tissue growth factor (CTGF) has been shown to be implicated in tumor development and progression. However, the role of CTGF in gastric cancer remains largely unknown. In this study, we showed that CTGF was highly expressed in gastric cancer tissues compared with matched normal gastric tissues. The CTGF expression in tumor tissue was associated with histologic grade, lymph node metastasis and peritoneal dissemination (P | 0.05). Patients with positive CTGF expression had significantly lower cumulative postoperative 5 year survival rate than those with negative CTGF expression (22.9% versus 48.1%, P | 0.001). We demonstrated that knockdown of CTGF expression significantly inhibited cell growth of gastric cancer cells and decreased cyclin D1 expression. Moreover, knockdown of CTGF expression also markedly reduced the migration and invasion of gastric cancer cells and decreased the expression of matrix metalloproteinase (MMP)-2 and MMP-9. Animal studies revealed that nude mice injected with the
Connective tissue growth factor (CTGF) is a mitogenic and chemotactic factor for cultured fibroblasts that has been implicated in wound healing, fibrotic disorders and uterine function. Although the primary translational products of the mouse, human and pig CTGF (mCTGF, hCTGF, pCTGF) genes are predi …
Connective tissue growth factor (CTGF) is a secreted protein that is strongly induced in human and experimental heart failure. CTGF is said to be profibrotic; however, the precise function of CTGF is unclear. We generated transgenic mice and rats with cardiomyocyte-specific CTGF overexpression (CTGF-TG). To investigate CTGF as a fibrosis inducer, we performed morphological and gene expression analyses of CTGF-TG mice and rat hearts under basal conditions and after stimulation with angiotensin II (Ang II) or isoproterenol, respectively. Surprisingly, cardiac tissues of both models did not show increased fibrosis or enhanced gene expression of fibrotic markers. In contrast to controls, Ang II treated CTGF-TG mice displayed preserved cardiac function. However, CTGF-TG mice developed age-dependent cardiac dysfunction at the age of 7 months. CTGF related heart failure was associated with Akt and JNK activation, but not with the induction of natriuretic peptides. Furthermore, cardiomyocytes from ...
A protein analysis using a mass spectrometry indicated that there are serum proteins showing significant quantitative changes after the administration of infliximab. Among them, connective tissue growth factor (CTGF) seems to be related to the pathogenesis of rheumatoid arthritis (RA). Therefore, this study was conducted to investigate how CTGF is associated with the disease progression of RA. Serum samples were collected from RA patients in active or inactive disease stages, and before or after treatments with infliximab. CTGF production was evaluated by ELISA, RT-PCR, indirect immunofluorescence microscopy, and immunoblotting. Osteoclastogenesis was evaluated using tartrate-resistant acid phosphatase (TRAP) staining, a bone resorption assay and osteoclasts specific catalytic enzymes productions. The serum concentrations of CTGF in RA were greater than in normal healthy controls and disease controls. Interestingly, those were significantly higher in active RA patients compared to inactive RA patients.
purpose. To investigate whether triamcinolone acetonide (TA) affects the expression of vascular endothelial growth factor (VEGF) and connective tissue growth factor (CTGF) in retinal pigment epithelial (RPE) cells exposed to oxidative stress.. methods. TA (10 nM, 1 μM, or 100 μM) was added to ARPE19 cells exposed to oxidative stress induced by hypoxia-reoxygenation and paraquat. Cellular expression of VEGF, CTGF, and an inducer of both growth factors, transforming growth factor (TGF)-β was investigated with real-time reverse transcription-polymerase chain reaction and Western blot analysis. Tube-forming assays were conducted with human umbilical vein endothelial cells (HUVECs) in conditioned medium from RPE cells exposed to oxidative stress, with or without TA treatment.. results. Oxidative stress induced mRNA expression of VEGF, CTGF, and TGF-β by RPE cells. TA reduced upregulation of VEGF and TGF-β in a concentration-dependent manner. In contrast, upregulation of CTGF by oxidative stress ...
Connective tissue growth factor (CTGF) is a secreted extracellular matrix-associated protein, which play a role in regulating various cellular functions. Althou
Regulation of connective tissue growth factor (ccn2*; ctgf) gene expression in human mesangial cells: modulation by HMG CoA reductase inhibitors (statins): Aim-
Purpose: : Recent studies have reported that connective tissue growth factor(CTGF) biochemically binds to fibronectin(FN) and promotes cell activity on various cells. In this study, we investigate the interaction between CTGF and FN for corneal epithelial cell attachment and migration. Methods: : A human corneal epithelial cells (HCEC) line that had been established by SV40-adenovirus recombinant vector was used in this study. CTGF-specific shRNA was synthesized and transfected into HCECs. The transfected cells were evaluated by western blot analysis to examine CTGF and FN production. For cell attachment assay, HCECs were either incubated with or without CTGF and were incubated in a FN-coated well or a BSA-coated well. After 45 minutes, the numbers of cells attached to the wells were counted. Another experiment was conducted to determine the interaction between CTGF and FN for epithelial cell migration. Rabbit cornea blocks were incubated with CTGF alone, FN alone or with a combination of both ...
Free Online Library: Connective tissue growth factor immunohistochemical expression is associated with gallbladder cancer progression.(Report) by Archives of Pathology & Laboratory Medicine; Health, general Cancer Analysis Development and progression Diagnosis Prognosis Cholecystitis Gallbladder cancer Genetic aspects Research Gene expression Physiological aspects Growth factors Immunohistochemistry
Li, G., Xie, Q., Shi, Y., Li, D., Zhang, M., Jiang, S., Zhou, H., Lu, H. and Jin, Y. (2006), Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats. J. Gene Med., 8: 889-900. doi: 10.1002/jgm.894 ...
Plasma and urinary levels of CTGF were significantly elevated in nondiabetic proteinuric CKD patients. Antiproteinuric intervention was associated with a stepwise reduction in urinary CTGF in proportion to the reduction in proteinuria but did not affect the elevated plasma CTGF levels.. CTGF is strongly implicated in diabetic renal fibrosis and injury (4-12). The elevated levels of CTGF in plasma and urine in our patients suggest that CTGF may also play a role in the pathophysiology of nondiabetic CKD and its extrarenal complications, as a biomarker and/or as a pathogenic factor.. The source of the urinary CTGF is of interest. Because of their small size (≤38 kD), CTGF and the fragments thereof are predicted to be cleared from plasma by glomerular filtration (21,22). Consequently, glomerular filtration of elevated plasma CTGF may be one of the causes of the elevated urinary CTGF levels in our patients who had a relatively preserved renal function. Second, the elevated urinary CTGF in our ...
This human anatomy ClipArt gallery offers 54 illustrations of human connective tissues, including fibrous connective tissue (e.g., ligaments, tendons), cartilage, osseous tissue, and adipose tissue.. ...
Regeneration of complex tissues, such as kidney, liver, and cartilage, continues to be a scientific and translational challenge. Survival of ex vivo cultured, transplanted cells in tissue grafts is among one of the key barriers. Meniscus is a complex tissue consisting of collagen fibers and proteoglycans with gradient phenotypes of fibrocartilage and functions to provide congruence of the knee joint, without which the patient is likely to develop arthritis. Endogenous stem/progenitor cells regenerated the knee meniscus upon spatially released human connective tissue growth factor (CTGF) and transforming growth factor-β3 (TGFβ3) from a three-dimensional (3D)-printed biomaterial, enabling functional knee recovery. Sequentially…. ...
The connective tissue growth factor gene (CTGF) is aberrantly expressed in 75% of precursor B-cell acute lymphoblastic leukaemias (pre-B ALL) and is associated with poor outcome. We identified consistent hypomethylation of the CTGF locus in primary pre-B ALL specimens regardless of CTGF expression. By contrast, primary T-cell ALL specimens, which do not express CTGF, exhibited distinctive patterns of hypermethylation. Furthermore, we confirmed that global changes in DNA methylation and histone acetylation can both functionally modulate CTGF expression in pre-B ALL cell lines. These data suggest that hypomethylation of the CTGF locus is an essential prerequisite for aberrant CTGF expression in pre-B ALL.. ...
Stromal mediators play a fundamental role in promoting mammary differentiation, and the contact between mammary epithelial cells and components of the basement membrane contributes to the prolactin-induced transcription of milk proteins [47]. Using the Tet-off system for inducible CTGF/CCN2 expression in the HC11 mouse mammary epithelial cell background, CTGF/CCN2 not only enhanced the early transcription of β-casein in response to lactogenic hormone, but also contributed to the formation of mammospheres, a hallmark of terminal differentiation in these cells. In addition, exogenous CTGF/CCN2 enhanced the formation of differentiated MCF10A acini in three dimensional cultures, extending the role of CTGF/CCN2 to another culture system which is likely to be structurally and physiologically relevant to the in vivo setting [42]. The role of CTGF/CCN2 in lactogenic differentiation may depend on its function in the expression and stabilization of matrix-integrin complexes, an activity which promotes ...
In addition to the activation of hepatic stellate cells TGF-β govern apoptosis and growth control of hepatocytes in liver injury. In non-parenchymal cells, TGF-β induces plasminogen activator inhibitor 1 (PAI-1) and connective tissue growth factor (CTGF) expression, which are involved in extra cellular matrix formation. Both genes were also regulated by glucocorticoids, which in certain cases showed antagonistic effects to the TGF-β-Smad 3 pathway. The purpose of our work was to investigate the influence of TGF-β and dexamethasone on PAI-1 and CTGF expression and secretion in primary hepatocytes. By examining PAI-1 and CTGF mRNA and protein expression in cell lysates and cell-conditioned media under the influence of TGF-β and dexamethasone, we analysed signalling pathways controlling their expression. TGF-β and dexamethasone significantly co-induce PAI-1 and CTGF protein expression. On the other hand, we showed that TGF-β diminished a glucocorticoid receptor dependent luciferase reporter signal
Complete information for CTGF gene (Protein Coding), Connective Tissue Growth Factor, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
This study provides the first evidence demonstrating the production of CTGF protein by glomerular cells and its role as a potentially important factor in the pathogenesis of diabetic glomerulosclerosis. In diabetic glomerulosclerosis, two major, well-known casual factors are hyperglycemia and glomerular hypertension. Recent studies have demonstrated that elevated glucose concentrations and hypertension-induced glomerular mechanical strain stimulate the overproduction of ECM, a change that is at least in part mediated through the metabolic action of TGF-β (13,15,16,22). CTGF, a newly described factor that promotes ECM deposition and fibrosis in the skin, appears to act downstream of TGF-β to induce ECM production, but whether TGF-β action is obligatory for its overexpression is uncertain. In addition, the role of CTGF in the development of glomerulosclerosis has not been studied. Here, we demonstrate that CTGF stimulates cultured MC to produce the ECM components fibronectin and collagen type ...
LRP1 is known to be a receptor for signal transmission and endocytosis. We formerly reported that LRP1 regulates WNT/β-catenin and protein kinase C signaling in chondrocytes and represses the hypertrophy of chondrocytes during endochondral ossification, and that LRP1 is co-localized with a ligand, CCN2, which conducts endochondral ossification, on chondrocytes. However, the role of LRP1 in endocytotic transport of CCN2 in chondrocytes is not yet understood. In the present study, we investigated the interaction between LRP1 and CCN2 during endocytotic trafficking.. RNAi-mediated knockdown of LRP1 in chondrocytic HCS-2/8 cells showed that the amount of exogenous CCN2 binding/incorporation was decreased in the LRP1 down-regulated cells. Importantly, we observed that CCN2 internalization in chondrocytes was dependent on clathrin and internalizated CCN2 was co-localized with an early or recycling endosome marker. Transcytosis of CCN2 through HCS-2/8 cells was confirmed by performing experiments with ...
The CCN2 protein is thought to consist of four separate modules. Module 1 is identical to insulin-like growth factor binding protein, module 2 consists of a chordin-like cysteine-rich domain, module 3 is composed of a thrombospondin type 1 domain and module 4 is designated as the CT module or cysteine knot. Modules 1 and 2 make up the N-terminal domain and are linked by a hinge region to the C-terminal domain which is made up of modules 3 and 4 [31, 32]. The ELISA analysis used in our study detects C-terminal CCN2 which translates into detection of both C-terminal fragments and whole CCN2. Our analysis is not able to detect N-terminal CCN2 fragments. Other studies have evaluated the utility of N-terminal CCN2 as a marker of intraocular fibrosis and in scleroderma. In these studies, neither C-terminal nor whole CCN2 were elevated in disease. As as result, it has been postulated that during a fibrotic response, in vivo CCN2 is cleaved resulting in N-terminal fragments which are excreted into the ...
In response to numerous pathological stimuli, such as hypertension and pressure loading, ECM proteins accumulate excessively in the heart. This process alters mechanical and electric properties, which adversely affects the function of the heart.2 The ECM of the healthy heart is subjected to a balanced turnover: on the one hand, new ECM components are synthesized by cardiac fibroblasts; and on the other hand, ECM components are continuously degraded by the action of a large family of matrix metalloproteinases, which are produced by multiple cell types in the heart (reviewed by Spinale27). The accumulation of ECM proteins in hearts of patients with LVH and heart failure has been attributed to an imbalance in the ratio of matrix metalloproteinases to their inhibitors (TIMPs) in favor of reduced proteolytic activity, as well as an increased production of ECM components by cardiac fibroblasts.27,28. CTGF, a profibrotic growth factor, is considered a key molecule in the control of ECM synthesis and ...
The present invention relates to antibodies that bind to CTGF. The antibodies are particularly directed to regions of CTGF involved in biological activities associated with fibrosis. The invention als
The concomitant overexpression of CTGF, collagen type I, TGF-beta1, and its signaling receptors in CP suggests that these proteins contribute to enhanced extracellular matrix synthesis and accumulation, resulting finally in the fibrogenesis observed in CP.
For the past 50 years, gastric cancer has been one of the ten most frequent cancers and the second leading cause of cancer-related death in the world. In Taiwan, it is the fifth most common cause of cancer-related deaths, accounting for 6.3% of all cancer deaths. The poor prognosis of gastric cancer is mostly caused by the extensive metastasis to the lymph nodes, liver, and peritoneal dissemination even if curative resection was performed. The main cause of recurrence after curative or noncurative resection of advanced tumors is peritoneal metastasis because of possible direct spillage and dissemination of tumor cells as a result of surgical manipulation, and it is associated with a poor prognosis. As yet, no effective treatment has been developed for this condition. The development of peritoneal metastasis is a multistep process, beginning with attachment to peritoneal mesothelial cells, retraction of the mesothelial cells and exposure of the basement membrane, attachment to the basement ...
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BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
In the Molecular Genetics Lab in Boyd Science Center, Kennedy and her colleagues-a mix of ambitious graduate and undergraduate biology students-have homed in on a particular gene called Connective Tissue Growth Factor (CTGF), which plays a key role in the bodys healing process. Following a heart attack, or in the case of cardiovascular disease, CTGF acts as a signaling molecule, telling different cytokines [proteins], Come here, we need to help fix this, explains Kennedy. The question is, are there differences in each persons CTGF gene that affect the levels of fibrosis severity that person develops?. Since she arrived at PSU in fall 2014, Kennedy has been working closely with her colleagues to answer that question. Shes been involved in just about every aspect of the research, including taking cell samples from healthy subjects across campus via cheek swab, extracting DNA from the cells, and amplifying (copying) regions of the CTGF gene so they can be sequenced. The sequencing is done ...
Connective tissue growth factor (CTGF/CCN2) has been shown previously to be aberrantly expressed in a high proportion of paediatric precursor B cell acute...
So much for the protective power of skin. The schistosome worm scoffs at this largest organ in the human body. When schistosome larvae are released from their intermediate hosts, freshwater snails, these parasites burrow through the unbroken skin of their ultimate hosts-humans who wade in tropical waters. Some schistosomes take up residence and lay eggs in the space around the hepatic portal vein, spurring liver inflammation that can persist for years. Five to ten percent of schistosome-infected individuals develop severe fibrosis of the liver, which often is fatal, but there is no way to identify these susceptible patients among the 350 million who are infected worldwide. Now, Dessein et al. report on their study of populations at risk for schistosomiasis-Chinese, Sudanese, and Brazilian subjects who live near worm-infested waters-which identified single-nucleotide polymorphisms (SNPs) around the connective tissue growth factor (CTGF) gene that correlate with liver fibrosis in schistosomiasis ...
Dysregulated wound healing is commonly associated with excessive fibrosis. Connective tissue growth factor (CTGF/CCN2) is characteristically overexpressed in fibrotic diseases and stimulated by transforming growth factor-β (TGF-β) in dermal fibroblasts. Reepithelialisation and epidermal wound coverage counteract excessive scar formation. We have previously shown that interleukin-1α (IL-1α) derived from keratinocytes conteracts TGF-β-stimulated CTGF-expression. The aim of this thesis was to further explore the effects of keratinocytes and IL-1α on gene and protein expression, as well as pathways, in TGF-β stimulated fibroblasts. Fibroblasts were studied in vitro by conventional two dimensional cell culture models and in a three dimensional keratinocyte-fibroblast organotypic skin culture model.. The results showed that IL-1 suppresses basal and TGF-β-induced CTGF mRNA and protein, involving a possible TAK1 mechanism. Keratinocytes regulate the expression of fibroblast genes important for ...
Subplate cells in the mouse are generally defined as cells located in the subplate layer between the white matter and layer 6a. They are some of the earliest born and maturing cells of the cerebral cortex. The postnatal subplate layer in mouse contains neurons with expression of the presynaptic protein complexin 3 (Cplx3), connective tissue growth factor (CTGF), the orphan nuclear receptor Nr4a2 (Nurr1), and the G-protein-coupled lysophosphatidic acid receptor 1 (Lpar1/Edg2). All 4 of these molecular markers show layer 6b-restricted expression at young postnatal ages, with CTGF expression being the most widespread in the young postnatal subplate. However, all 4 markers overlap in their expression pattern to varying degrees. Here we demonstrate with bromodeoxyuridine birthdating that cells labeled with any 1 of these molecular subplate markers are indeed generated at E11.5 or E12.5 in the mouse. Furthermore, we demonstrate a correlation between gene expression and cell birthdates. Lpar1-GFP cells are
TY - JOUR. T1 - CCN2 as a novel molecule supporting energy metabolism of chondrocytes. AU - Maeda-Uematsu, Aya. AU - Kubota, Satoshi. AU - Kawaki, Harumi. AU - Kawata, Kazumi. AU - Miyake, Yoshiaki. AU - Hattori, Takako. AU - Nishida, Takashi. AU - Moritani, Norifumi. AU - Lyons, Karen M.. AU - Iida, Seiji. AU - Takigawa, Masaharu. PY - 2014/5. Y1 - 2014/5. N2 - CCN2/connective tissue growth factor (CTGF) is a unique molecule that promotes both chondrocytic differentiation and proliferation through its matricellular interaction with a number of extracellular biomolecules. This apparently contradictory functional property of CCN2 suggests its certain role in basic cellular activities such as energy metabolism, which is required for both proliferation and differentiation. Comparative metabolomic analysis of costal chondrocytes isolated from wild-type and Ccn2-null mice revealed overall impaired metabolism in the latter. Among the numerous metabolites analyzed, stable reduction in the intracellular ...
One potential model of type 2 diabetes etiology is that those with the disease were born with less β-cell mass making them susceptible to stressors such as obesity. Thus, it would be of therapeutic potential to find mechanisms that increase functional β-cell mass. One candidate that has been studied in our lab is Connective tissue growth factor (Ctgf). Ctgf is a secreted protein known to be involved in cell adhesion, migration and, in some cell types, proliferation. Previous studies in our lab have shown that Ctgf is crucial for β-cell development, with loss of Ctgf resulting in fewer β-cells and decreased β-cell proliferation, thus decreased β-cell mass at birth. Ctgf is also important in situations of metabolic stress, such as pregnancy or β-cell loss. Haploinsufficiency during pregnancy results in decreased maternal β-cell proliferation while in contrast, over-expression of Ctgf results in increased β-cell proliferation and regeneration in a model of partial β-cell ablation. ...
Background This study elucidates exposure-response relationships between performance of repetitive tasks, grip strength declines, and fibrogenic-related protein changes in muscles, and their link to inflammation. Specifically, we examined forearm flexor digitorum muscles for changes in connective tissue growth factor (CTGF; a matrix protein associated with fibrosis), collagen type I (Col1; a matrix component), and transforming growth factor beta 1 (TGFB1; an upstream modulator of CTGF and collagen), in rats performing one of two repetitive tasks, with or without anti-inflammatory drugs. Methodology/Results To examine the roles of force versus repetition, rats performed either a high repetition negligible force food retrieval task (HRNF), or a high repetition high force handle-pulling task (HRHF), for up to 9 weeks, with results compared to trained only (TR-NF or TR-HF) and normal control rats. Grip strength declined with both tasks, with the greatest declines in 9-week HRHF rats. Quantitative ...
Epithelial-mesenchymal transition (EMT) contributes significantly to interstitial matrix deposition in diabetic kidney disease (DKD). However, detection of EMT in kidney tissue is impracticable, and anti-EMT therapies have long been hindered. We reported that phosphatase and tensin homolog (PTEN) promoted transforming growth factor beta 1 (TGF-β), sonic hedgehog (SHH), connective tissue growth factor (CTGF), interleukin 6 (IL-6), and hyperglycemia-induced EMT when PTEN was modified by a MEX3C-catalyzed K27-linked polyubiquitination at lysine 80 (referred to as PTENK27-polyUb). Genetic inhibition of PTENK27-polyUb alleviated Col4a3 knockout-, folic acid-, and streptozotocin-induced (STZ-induced) kidney injury. Serum and urine PTENK27-polyUb concentrations were negatively correlated with glomerular filtration rate (GFR) for diabetic patients. Mechanistically, PTENK27-polyUb facilitated dephosphorylation and protein stabilization of TWIST, SNAI1, and YAP in renal epithelial cells, leading to ...
YAP1 is a transcriptional co-activator[17] and its proliferative and oncogenic activity is driven by its association with the TEAD family of transcription factors,[13] which up-regulate genes that promote cell growth and inhibit apoptosis.[18] Several other functional partners of YAP1 were identified, including RUNX,[17] SMADs,[19][20] p73,[21] ErbB4,[22][23] TP53BP,[24] LATS1/2,[25] PTPN14,[26] AMOTs,[27][28][29][30] and ZO1/2.[31] YAP1 and its close paralog, TAZ (WWTR1), are the main effectors of the Hippo tumor suppressor pathway.[32] When the pathway is activated, YAP1 and TAZ are phosphorylated on a serine residue and sequestered in the cytoplasm by 14-3-3 proteins.[32] When the Hippo pathway is not activated, YAP1/TAZ enter the nucleus and regulate gene expression.[32] It is reported that several genes are regulated by YAP1, including Birc2, Birc5, connective tissue growth factor (CTGF), amphiregulin (AREG), Cyr61, Hoxa1 and Hoxc13. YAP/TAZ have also been shown to act as stiffness sensors, ...
The discovery, published in Nature Communications, may also provide a means of homing drugs to diseased areas of the brain to treat AD, Parkinsons disease, as well as glioblastoma, brain injuries and stroke, medicalxpress.com wrote.. Aman Mann, PhD, research assistant professor at SBP who shares the lead authorship of the study with Pablo Scodeller, PhD, a postdoctoral researcher at SBP, said, Our goal was to find a new biomarker for AD.. We have identified a peptide (DAG) that recognizes a protein that is elevated in the brain blood vessels of AD mice and human patients.. The DAG target, connective tissue growth factor (CTGF) appears in the AD brain before amyloid plaques, the pathological hallmark of AD.. CTGF is a protein that is made in the brain in response to inflammation and tissue repair.. Our finding that connects elevated levels of CTGF with AD is consistent with the growing body of evidence suggesting that inflammation plays an important role in the development of AD.. The ...
Adenosine, acting through the A2A receptor, promotes tissue matrix production in the skin and the liver and induces the development of dermal fibrosis and cirrhosis in murine models. Since expression of A2A receptors is increased in scleroderma fibroblasts, we examined the mechanisms by which the A2A receptor produces its fibrogenic effects. The effects of A2A receptor ligation on the expression of the transcription factor, Fli1, a constitutive repressor for the synthesis of matrix proteins, such as collagen, is studied in dermal fibroblasts. Fli1 is also known to repress the transcription of CTGF/CCN2, and the effects of A2A receptor stimulation on CTGF and TGF-β1 expression are also examined. A2A receptor occupancy suppresses the expression of Fli1 by dermal fibroblasts. A2A receptor activation induces the secretion of CTGF by dermal fibroblasts, and neutralization of CTGF abrogates the A2A receptor-mediated enhancement of collagen type I production. A2AR activation, however, resulted in a decrease
Background: Massague et al have shown that breast cancer cell line subpopulations with elevated bone metastatic activity overexpress chemokine receptor 4 (CXCR4), interleukin 11 (IL11), osteopontin (OPN) and connective tissue growth factor (CTGF) (Cancer Cell 3:537, 2003). CXCR4 overexpression results in bone-homing and extravasation of tumor cells in bone. In MA.14, we found that serum β-CTx was associated with bone-only relapse while Basik et.al showed that higher serum stromal cell-derived factor 1 (SDF-1) (ligand for CXCR-4) levels were associated with worse overall event-free survival (EFS) (ASCO 2010). In this study, we examined concurrently the association of both β-CTx and serum SDF-1 with bone relapse.. Methods: Serum β-CTx (Serum CrossLaps, Nordic Biosciences, Copenhagen, DN) was determined in pretreatment sera from 621 of 667 NCIC CTG MA.14 patients. SDF-1 (CXCL12) (R&D Systems, Minneapolis, MN) levels were successfully determined in the 4 month post-treatment serum (SDF-1) for 508 ...
1. J.M. Schober, N. Chen, T.M. Greszkiewicz, I. Jovanovic, E.E. Emeson, T.P. Ugarova, R.D. Ye, L.F. Lau, S.C.-T. Lam. Identification of Integrin aMb2 as an Adhesion Receptor on Peripheral Blood Monocytes for Cyr61 (CCN1) and Connective Tissue Growth Factor (CCN2): Immediate-early Gene Products Expressed in Atherosclerotic Lesions. Blood. 2002;99:4457-4465. Pubmed ...
Beddy, D.,Mulsow, J.,Watson, R. W.,Fitzpatrick, J. M.,OConnell, P. R.; (2006) Expression and regulation of connective tissue growth factor by transforming growth factor beta and tumour necrosis factor alpha in fibroblasts isolated from strictures in patients with Crohns disease. British Journal of Surgery, 93 (10):1290-6. ...
Novel genes encoding proteins having prognostic, diagnostic, preventive, therapeutic and other uses - The invention relates to the discovery and characterization of several genes and the polypeptides they encode: thymotaxin (Tango-45), Tango-63d, Tango-63e, Tango-67, and huchordin (Tango-66). Thymotaxin is a new member of the C-C family of chemokines. Tango-63d and Tango-63e are two novel polypeptides within the tumor necrosis factor (TNF) receptor superfamily. Tango-67 is related to a number of growth factors, particularly members of the connective tissue growth factor family. Huchordin is related to chordin, a known protein that is involved in the induction of twinned axes, can completely rescue axial development in ventralized embryos, is a potent dorsalizing factor, and plays a crucial role in regulating cell-cell interactions in the organizing centers of head, trunk, and tail development. The invention encompasses nucleic acid molecules encoding nucleic acids and polypeptides of the ...
The major structural change in the human trabecular meshwork (TM) of eyes with primary open-angle glaucoma (POAG) is an increase in extracellular matrix (ECM) in the juxtacanalicular region of the TM. There is evidence that treatment with TGF-beta2 causes an induction of ECM deposition in cultured human TM cells and that TGF-beta2 is causatively involved in the JCT ECM increase in POAG. In the present study, we investigated the effects of connective tissue growth factor (CTGF) on the biology of cultured human TM cells. CTGF is a downstream mediator of TGF-beta2-signaling, which is expressed at high amounts in the human TM in situ. HEK293 cells were transfected with an eukaryotic expression plasmid containing the coding sequences of human CTGF. Secreted CTGF was isolated and purified by chromatography. Primary human TM cells were incubated for 24 h with CTGF at concentrations of 2.5-100 ng/ml. Following treatment with CTGF, the expression of various ECM components that are expressed in the JCT, ...
Deposition of extracellular matrix (ECM) in trabecular meshwork, such as fibronectin, collagen IV, elastin. leads to increased resistance of trabecular meshwork in primary open angle glaucoma (POAG). Connective tissue growth factor (CTGF) is known to regulate the ECM deposits. In this study, we detect the effect of adenovirus conducted CTGF (Adv-CTGF) transfection on either the expression of ECM components or aqueous humor outflow facility. Adv-CTGF was used to transfect rat trabecular meshwork cells in vivo and in vitro. Aqueous humor outflow facility was test by microbeads perfusion. Protein expression of CTGF, fibronectin, and collagen IV was determined using Western blot. In the Adv-CTGF group, the outflow facility displayed a significant decrease from baseline. It appears as though the transfection with Adv-CTGF significantly affects the aqueous humor outflow pattern. A negative correlation between IOP and PEFL indicated that a decrease in the area of bead deposition corresponded to an overall
Background. hepatectomy was conducted to evaluate the expression of candidate genes at the protein level using immunohistochemistry on tissue microarrays (TMAs). Of the 350 patients 273 (78.0%) were infected with HBV. Results. Seven intratumoral genes and 17 peritumoral genes were overexpressed in patients with BM whereas 15 intratumoral genes and 28 peritumoral genes were underexpressed in patients with BM. We selected the following four genes for further analysis because they were differentially expressed in the cancer gene-specific microarray and were previously reported to be associated with BM: connective tissue growth factor (gene we used 500 nM of the forward primer (5′-GTACGCTGTGAAGGCATCAA-3′) and reverse primer (5′-GTTGGTGTTCATCCGCTTG-3′) and 25 μL of SYBR Rabbit Polyclonal to KR2_VZVD. Green PCR Master Mix (Applied Biosystems Inc Foster City CA). The total PCR volume was 50 μL. PCR amplifications consisted of a short enzyme activation stage at 95°C for 12 mins accompanied by ...
Pulmonary and pleural fibrosis is one of pathological characterized by Mycobacterium tuberculosis (M. tb) infection in the lungs. When the lungs are infected with M. tb, the recruitment of immune cells and fibroblasts leads to granuloma formation and fibrotic scaring in interstitial lung tissue, which in turns causes irreversible loss of pulmonary function and pulmonary failure. Several studies indicated that the fibrotic factors, such as transforming growth factor- (TGF-), were increased in pleural effusion and serum of patient with tuberculosis. The expression of connective tissue growth factor (CTGF), a fibrotic factor, has been reported in lung fibrosis. However, the mechanism of M. tb-induced CTGF expression in human lung fibroblasts is still unclear. Fibrocytes are unique bone marrow-derived mesenchymal progenitor cells found in circulation. Fibrocytes have been shown to play an important role in wound healing following injury and in the generation of pulmonary fibrosis. Previous ...
TY - JOUR. T1 - Experimental oligohydramnios decreases collagen in hypoplastic fetal rat lungs. AU - Chen, Chung Ming. AU - Chou, Hsiu Chu. AU - Wang, Leng-Fang. AU - Lang, Yaw Dong. PY - 2008/11. Y1 - 2008/11. N2 - Neonates with premature rupture of the membrane and oligohydramnios have an increased risk of acute respiratory morbidity. The aims of this study are to investigate the effects of experimental oligohydramnios on transforming growth factor (TGF)-β and connective tissue growth factor (CTGF) expressions and collagen level in fetal rat lungs. On day 16 of gestation, we anesthetized timed pregnant Sprague-Dawley dams, punctured the uterine wall and fetal membranes of each amniotic sac which resulted in oligohydramnios. Fetuses in the opposite uterine horn served as controls. On days 19 and 21 of gestation, fetuses were delivered by cesarean section. Rats exposed to oligohydramnios exhibited significantly lower lung weight/body weight ratios on days 19 and 21 of gestation than did the ...
Systemic sclerosis (SSc), or scleroderma, similar to many fibrotic disorders, lacks effective therapies. Current trials focus on anti-inflammatory drugs or targeted approaches aimed at one of the many receptor mechanisms initiating fibrosis. In light of evidence that a myocardin-related transcription factor (MRTF)-and serum response factor (SRF)-regulated gene transcriptional program induced by Rho GTPases is essential for myofibroblast activation, we explored the hypothesis that inhibitors of this pathway may represent novel antifibrotics. MRTF/SRF-regulated genes show spontaneously increased expression in primary dermal fibroblasts from patients with diffuse cutaneous SSc. A novel small-molecule inhibitor of MRTF/SRF-regulated transcription (CCG-203971) inhibits expression of connective tissue growth factor (CTGF), α-smooth muscle actin (α-SMA), and collagen 1 (COL1A2) in both SSc fibroblasts and in lysophosphatidic acid (LPA)-and transforming growth factor β (TGFβ)-stimulated fibroblasts. ...
TY - JOUR. T1 - GLUT1 regulation of the pro-sclerotic mediators of diabetic nephropathy. AU - Heilig, Charles W.. AU - Deb, Dilip K.. AU - Abdul, Afu. AU - Riaz, Hasan. AU - James, Leighton R.. AU - Salameh, Jamal. AU - Nahman, N. Stanley. PY - 2013/4/12. Y1 - 2013/4/12. N2 - Diabetic glomerulosclerosis is characterized by accumulation of extracellular matrix proteins, mesangial expansion, and tubulointerstitial fibrosis. Hyperglycemia accelerates development of the disease, a direct result of increased intracellular glucose availability. The facilitative glucose transporter GLUT1 mediates mesangial cell glucose flux which leads to activation of signaling cascades favoring glomerulosclerosis, including pathways mediated by angiotensin II (Ang II), transforming growth factor β (TGF-β), connective tissue growth factor (CTGF), and vascular endothelial growth factor (VEGF). Ang II has both hemodynamic and metabolic effects directly inducing GLUT1 and/or matrix protein synthesis through diacyl ...
Purpose Recent research indicated undisputed contribution of connective tissues growth aspect (CTGF) in the development of several cancers including non-small cell lung cancer (NSCLC). on CTGF transcript and proteins amounts in NSCLC cells (A549 Calu-1). DNA methylation position from the regulatory area was examined by bisulfite sequencing. The influence of 5-dAzaC and TSA on NSCLC cells proliferation and viability was monitored with the trypan blue assay. Results We discovered significantly decreased degrees of CTGF mRNA and proteins (both occurred irrespective of gender in every histological subtypes of NSCLC. Furthermore we showed that 5-dAzaC and TSA could actually restore CTGF proteins and mRNA items in NSCLC cells. Zero methylation within regulatory area was detected Nevertheless. Both compounds reduced NSCLC cells proliferation significantly. Conclusions Decreased appearance of is normally a common feature in NSCLC; nonetheless it could be restored with the chromatin-modifying realtors ...
A family of growth regulators (originally called cef10, connective tissue growth factor, fisp-12, cyr61, or, alternatively, beta IG-M1 and beta IG-M2), all belong to immediate-early genes expressed after induction by growth factors or certain oncogenes. Sequence analysis of this family revealed the presence of four distinct modules. Each module has homologues in other extracellular mosaic proteins such as Von Willebrand factor, slit, thrombospondins, fibrillar collagens, IGF-binding proteins and mucins. Classification and analysis of these modules suggests the location of binding regions and, by analogy to better characterised modules in other proteins, sheds some light onto the structure of this new family [(PUBMED:7687569)].. The vWF domain is found in various plasma proteins: complement factors B, C2, CR3 and CR4; the integrins (I-domains); collagen types VI, VII, XII and XIV; and other extracellular proteins [(PUBMED:8412987), (PUBMED:8145250), (PUBMED:1864378)]. Although the majority of ...
A family of growth regulators (originally called cef10, connective tissue growth factor, fisp-12, cyr61, or, alternatively, beta IG-M1 and beta IG-M2), all belong to immediate-early genes expressed after induction by growth factors or certain oncogenes. Sequence analysis of this family revealed the presence of four distinct modules. Each module has homologues in other extracellular mosaic proteins such as Von Willebrand factor, slit, thrombospondins, fibrillar collagens, IGF-binding proteins and mucins. Classification and analysis of these modules suggests the location of binding regions and, by analogy to better characterised modules in other proteins, sheds some light onto the structure of this new family [(PUBMED:7687569)].. The vWF domain is found in various plasma proteins: complement factors B, C2, CR3 and CR4; the integrins (I-domains); collagen types VI, VII, XII and XIV; and other extracellular proteins [(PUBMED:8412987), (PUBMED:8145250), (PUBMED:1864378)]. Although the majority of ...
Sphingosine-1-phosphate (S1P) regulates a broad spectrum of fundamental cellular processes like proliferation, death, migration and cytokine production. Therefore, elevated levels of S1P may be causal to various pathologic conditions including cancer, fibrosis, inflammation, autoimmune diseases and aberrant angiogenesis. Here we report that S1P lyase from the prokaryote Symbiobacterium thermophilum (StSPL) degrades extracellular S1P in vitro and in blood. Moreover, we investigated its effect on cellular responses typical of fibrosis, cancer and aberrant angiogenesis using renal mesangial cells, endothelial cells, breast (MCF-7) and colon (HCT 116) carcinoma cells as disease models. In all cell types, wild-type StSPL, but not an inactive mutant, disrupted MAPK phosphorylation stimulated by exogenous S1P. Functionally, disruption of S1P receptor signaling by S1P depletion inhibited proliferation and expression of connective tissue growth factor in mesangial cells, proliferation, migration and VEGF ...
Blindness is a devastating consequence of PVDs such as PDR and PVR. Currently, the progression of these diseases cannot be effectively prevented, and the treatment options are limited to vitreoretinal surgery. An effective pharmacological treatment is thus urgently needed to complement or potentially replace the surgical intervention. In the current study, we show statins novel function in inhibiting the Rho/Rho-kinase pathway, the contraction of collagen gel, and the progression of experimental PVR, suggesting the therapeutic potential of these compounds for the treatment of PVDs.. Various cytokines, such as TGF-β, connective tissue growth factor, interleukin-6, and platelet-derived growth factor (PDGF), are overexpressed in the vitreous and membranes associated with PDR and PVR, and they contribute to the pathogenesis of these diseases (15,37-40). Among these cytokines, TGF-β2 induces the transformation of retinal pigment epithelial cells or hyalocytes to myofibroblastic cells (18,41) and ...
Glucosamine is a compound known as an aminosugar, and is naturally concentrated in human connective tissue and joint synovial fluid. Several forms of glucosamine are available. However, glucosamine sulfate appears to have superior absorption and effectiveness.. The sulfur portion of the compound also has nutritional value since the body can use it to produce disulfate bonds that further contribute to the strength and integrity of connective tissue. In fact, recent studies have shown that glucosamine sulfate supplementation may be as effective as nonsteroidal anti-inflammatory drugs (NSAIDs) for relief of arthritis pain and inflammation.. Chondroitin sulfate (CS) is a mucopolysaccharide and natural constituent of cartilage, connective tissue and bone. Clinical research has shown that when taken as a nutritional supplement, CS can provide several important compounds vital to healthy connective tissue. CS has been shown to produce positive results in a variety of joint complaints, and has gained ...
Question - Have white tissue growth under upper lip inside. What could it be?. Ask a Doctor about Salivary gland, Ask a General & Family Physician
The study expands on work reported in 2001 in the journal Molecular Cell announcing that a team led by Buck had found that a small piece of an important regulatory protein called C/EBP beta was responsible for fibrous tissue growth, or excessive scar tissue following injury or illness. When normal scarring goes awry, excessive build-up of fibrous tissue can produce disfiguring scars or clog vital internal organs and lead to serious complications. Buck and colleagues developed a mutated protein that stopped this excessive fibrous tissue growth ...
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Angiogenesis is an essential process during normal tissue growth and in response to wound healing. It is vital for the development of new connective tissue and small blood vessels from pre-existing vessels.
For optimum tissue growth, the growth area is opto-mechanical treated. This enhances cell adhesion and cell growth.. The geometry of the neck guarantees total access of TPP pipettes, TPP scrappers and the TPP spatula. No dead corners = 100 % cell retrieval.. Additional advantages:. ...
This project is aimed at understanding the genetic pathways that tissue growth and organ size in vivo. Previous studies in our laboratory have utilized a screen...
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Sp1 is a transcription factor most closely studied in this context. Apart from TGFβ, connective tissue growth factor (CTGF) has ... A significant player in the process is transforming growth factor (TGFβ). This protein appears to be overproduced, and the ... Kidneys Kidney involvement, in scleroderma, is considered a poor prognostic factor and frequently a cause of death. The most ... Patients with greater cutaneous involvement are more likely to have involvement of the internal tissues and organs. Most ...
"Role and interaction of connective tissue growth factor with transforming growth factor-beta in persistent fibrosis: A mouse ... cleave connective tissue growth factor and reactivate angiogenic activity of vascular endothelial growth factor 165". J. Biol. ... "Selective expression of connective tissue growth factor in fibroblasts in vivo promotes systemic tissue fibrosis". Arthritis ... Nagashima T, Kim J, Li Q, Lydon JP, DeMayo FJ, Lyons KM, Matzuk MM (October 2011). "Connective tissue growth factor is required ...
"Modulation of the expression of connective tissue growth factor by alterations of the cytoskeleton". The Journal of Biological ... this relation exists for connective tissue growth factor. Biology portal Microtentacle Orchestrated objective reduction - a ... Signals sent between the follicular cells and the oocyte (such as factors similar to epidermal growth factor) cause the ... This switch from growth to shrinking is called a catastrophe. GTP-bound tubulin can begin adding to the tip of the microtubule ...
2004). "Connective tissue growth factor and its role in lung adenocarcinoma invasion and metastasis". J. Natl. Cancer Inst. 96 ... 1997). "A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue ... encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth ...
... connective tissue growth factor, Cyr61/Cef10, and neuroblastoma overexpressed gene) family, suppresses In vivo tumor growth and ... Xie D, Nakachi K, Wang H, Elashoff R, Koeffler HP (Dec 2001). "Elevated levels of connective tissue growth factor, WISP-1, and ... "Overexpression of connective tissue growth factor WISP-1 in Chinese primary rectal cancer patients". World Journal of ... "WISP genes are members of the connective tissue growth factor family that are up-regulated in wnt-1-transformed cells and ...
"Effect of connective tissue growth factor on hypoxia-inducible factor 1alpha degradation and tumor angiogenesis". Journal of ... and hypoxia-inducible factor 1alpha in tumor growth and metastasis". Journal of the National Cancer Institute. 102 (6): 426-42 ... Pang AL, Clark J, Chan WY, Rennert OM (November 2011). "Expression of human NAA11 (ARD1B) gene is tissue-specific and is ... Lim JH, Chun YS, Park JW (1 July 2008). "Hypoxia-inducible factor-1alpha obstructs a Wnt signaling pathway by inhibiting the ...
"The Kruppel-like factor KLF15 inhibits connective tissue growth factor (CTGF) expression in cardiac fibroblasts". J. Mol. Cell ... Krüppel-like factor 15 is a protein that in humans is encoded by the KLF15 gene in the Krüppel-like factor family. Its former ... Transforming growth factor-beta1 (TGFbeta1) strongly reduces KLF15 expression. Adenoviral overexpression of KLF15 inhibits ... KLF15 in adipose tissue is down-regulated in obese mice. aP2-KLF15 Tg mice which overexpress KLF15 manifest insulin resistance ...
"The modular architecture of a new family of growth regulators related to connective tissue growth factor". FEBS Lett. 327 (2): ...
"Connective tissue growth factor modulates oral squamous cell carcinoma invasion by activating a miR-504/FOXP1 signalling". ...
Yang MH, Lin BR, Chang CH, Chen ST, Lin SK, Kuo MY, Jeng YM, Kuo ML, Chang CC (May 2012). "Connective tissue growth factor ... activated transcription factor X-box-binding protein 1 (XBP1) induces microRNA-346 expression that targets the human antigen ...
Xie D, Nakachi K, Wang H, Elashoff R, Koeffler HP (2001). "Elevated levels of connective tissue growth factor, WISP-1, and ... including stimulation by platelet-derived growth factor and basic fibroblast growth factor, transforming growth factor β1 (TGF- ... connective tissue growth factor, or CCN2), and NOV (nephroblastoma overexpressed, or CCN3). These proteins, together with WISP1 ... and connective tissue growth factor (CCN2), immediate-early gene products expressed in atherosclerotic lesions". Blood. 99 (12 ...
Sp1 is a transcription factor most closely studied in this context. Apart from TGFβ, connective tissue growth factor (CTGF) has ... Systemic scleroderma, also called diffuse scleroderma or systemic sclerosis, is an autoimmune disease of the connective tissue ... A significant player in the process is transforming growth factor (TGFβ). This protein appears to be overproduced, and the ... a b c University of maryland Medical Center , Medical Reference , Patient Education , Scleroderma - Risk Factors Harvey Simon, ...
"Identification of paraxanthine as the most potent caffeine-derived inhibitor of connective tissue growth factor expression in ... As a result, it is responsible for increased transport of potassium ions into skeletal muscle tissue. Similarly, the compound ...
January 2009). "miR-133 and miR-30 regulate connective tissue growth factor: implications for a role of microRNAs in myocardial ... "The miR-30 miRNA family regulates Xenopus pronephros development and targets the transcription factor Xlim1/Lhx1". Development ...
It is reported that several genes are regulated by YAP1, including Birc2, Birc5, connective tissue growth factor (CTGF), ... tissue homeostasis. • heart process. • positive regulation of cardiac muscle cell proliferation. • cardiac muscle tissue ... transcription factor activity, RNA polymerase II transcription factor binding. • chromatin binding. • transcription coactivator ... transcription factor complex. • cytosol. • membrane. Biological process. • regulation of transcription, DNA-templated. • ...
... mesangial cells can respond by producing several growth factors: TGF-1, VEGF and connective tissue growth factor. The mesangium ... Mice lacking the growth factor PDGF-B or PDGFRβ do not develop mesangial cells. When mesangial cells are absent the blood ... Endothelial precursor cells secrete platelet-derived growth factor (PDGF)-B and mesangial cells have receptors for PDGF. This ... The transcription factor for PDGFRβ, Tbx18, is crucial for the development of mesangial cells. Without Tbx18 the development of ...
Connective tissue growth factor is involved in the pathogenesis of fibrotic diseases and is predominantly found in PaSCs ... The production of these factors is regulated by fibroblast growth factor 2, TGF-β1, and PDGF. In addition to cytokine-mediated ... Protein kinases such as MAPKs are primary mediators of activating signals initiated by the growth factors, angiotensin II and ... Hypoxia also stimulates nuclear expression of HIF-1α followed by the production of vascular endothelial growth factor (VEGF) in ...
Castor C, Furlong A, Carter-Su C (1985). "Connective tissue activation: stimulation of glucose transport by connective tissue ... a major human platelet-derived growth factor.". Proc. Natl. Acad. Sci. U.S.A. 80 (3): 765-9. PMC 393460. PMID 6572368. doi: ... Castor CW, Furlong AM, Carter-Su C (1985). "Connective tissue activation: stimulation of glucose transport by connective tissue ... 1989). "Connective tissue activation. XXXIII. Biologically active cleavage products of CTAP-III from human platelets.". Biochem ...
... cytokines and growth factors from surrounding connective tissues and active skeletal muscles. Notably, HGF, a cytokine, is ... It is thought that HGF activates satellite cells, while insulin-like growth factor-I (IGF-1) and fibroblast growth factor (FGF ... Myocyte nuclear factor (MNF), and c-met proto-oncogene (receptor for hepatocyte growth factor (HGF)) are less commonly used ... More specifically, IGF-1 exists in two isoforms: mechano growth factor (MGF) and IGF-IEa. While the former induces activation ...
... "α-Tocopherol induces expression of connective tissue growth factor and antagonizes tumor necrosis factor-α-mediated ... Gyorgy, P.; Rose (1948). "Effect of dietary factors on early mortality and hemoglobinuria in rats following administration of ... á expresión do factor de crecemento do tecido conectivo (CTGF).[17][18] Cando se expresa o xene do CTGF, é responsable da ...
... deactivation of receptors to certain cytokines and inhibition of connective tissue growth factor (CTGF). In cases of advanced ... communication and tissue differentiation. This leads to irregular tissue growth in organs and various other diseases. 83% of ... The build-up of tissue in the liver, known as hepatic fibrosis, may cause symptoms such as jaundice, ascites, abnormal bleeding ... Patients are often within the lower percentiles for height and weight growth. Hypotonia is a possible sign of COACH syndrome. ...
1999). "Identification and cloning of a connective tissue growth factor-like cDNA from human osteoblasts encoding a novel ... growth factor II binding site to amino acids 1508-1566 in repeat 11 of the mannose 6-phosphate/insulin-like growth factor II ... O'Dell SD, Day IN (1998). "Insulin-like growth factor II (IGF-II)". Int. J. Biochem. Cell Biol. 30 (7): 767-71. PMID 9722981. ... 1987). "Insulin-like growth factor II receptor as a multifunctional binding protein". Nature 329 (6137): 301-7. PMID 2957598. ...
Transforming growth factor beta (TGF-β) plays an important role in MFS. Fibrillin-1 directly binds a latent form of TGF-β, ... MFS is caused by a mutation in FBN1, one of the genes that makes fibrillin, which results in abnormal connective tissue. It is ... Dural ectasia, the weakening of the connective tissue of the dural sac encasing the spinal cord, can result in a loss of ... Marfan syndrome (MFS) is a genetic disorder that affects the connective tissue. Those with the condition tend to be tall and ...
Dysregulation of NGF signaling has also been linked to Alzheimer's disease.[31][32][33][34][35][36] Connective tissue cells ... nerve growth factor receptor binding. • growth factor activity. • signaling receptor binding. Cellular component. • endosome. • ... Nerve growth factor (NGF) is a neurotrophic factor and neuropeptide primarily involved in the regulation of growth, maintenance ... "Human CD4+ T cell clones produce and release nerve growth factor and express high-affinity nerve growth factor receptors". The ...
... connective tissue growth factor, or CCN2), and NOV. These proteins, together with WISP1 (CCN4), WISP2 (CCN5), and WISP3 (CCN6) ... Forced expression of NOV inhibits proliferation and restores growth control in CML cells, suggesting that NOV may be an ... secretory signal peptide followed by four structurally distinct domains with homologies to insulin-like growth factor binding ... Davies W (Nov 2019). "An analysis of Cellular Communication Network Factor Proteins as candidate mediators of postpartum ...
... connective tissue growth factor or CCN2), and NOV (nephroblastoma overexpressed or CCN3). Together with three Wnt-induced ... connective tissue growth factor) CCN3: NOV (nephroblastoma overexpressed) CCN4: WISP1 (WNT1 inducible signaling pathway protein ... which include the insulin-like growth factor-binding domain (IGFBP), the Von Willebrand factor type C domain (VWC), the ... Huang W, Pal A, Kleer CG (March 2012). "On how CCN6 suppresses breast cancer growth and invasion". J Cell Commun Signal. 6 (1 ...
... connective tissue growth factor, or CCN2), and NOV (nephroblastoma overexpressed, or CCN3). These proteins, together with WISP1 ... Nakamura Y, Cui Y, Fernando C, Kutz WE, Warman ML (June 2009). "Normal growth and development in mice over-expressing the CCN ... Huang W, Pal A, Kleer CG (March 2012). "On how CCN6 suppresses breast cancer growth and invasion". J Cell Commun Signal. 6 (1 ... secretory signal peptide followed by four structurally distinct domains with homologies to insulin-like growth factor binding ...
... connective tissue growth factor CTI - (i) Co-operative Target Identification CTIL - (i) Critical Tracked Items List CTIS - (i) ...
Platelet-derived growth factors are important in connective tissue growth, survival and function, and consist of disulphide- ... 2003). "Platelet-derived growth factor (PDGF)-C, a PDGF family member with a vascular endothelial growth factor-like structure ... 2005). "Regulation of fibrogenic/fibrolytic genes by platelet-derived growth factor C, a novel growth factor, in human dermal ... "Platelet-derived growth factor C (PDGF-C), a novel growth factor that binds to PDGF alpha and beta receptor". J. Biol. Chem. ...
Connective tissue cells. Hidden categories: *All articles with unsourced statements. *Articles with unsourced statements from ... This is a type of safeguard to the system, almost like a two-factor authentication method. First, the B cells have to encounter ... the activation and growth of B cell clones able to secrete antibodies of higher affinity for the antigen. ... Differentiation of mature B cells into plasma cells is dependent upon the transcription factors Blimp-1/PRDM1 and IRF4. ...
High levels of growth hormone (GH) and insulin-like growth factor 1 (IGF-1) are also associated with worsened acne.[42] Both ... skin discoloration and connective tissue damage from the accumulation of homogentisic acid).[35] ... Another common factor is the excessive growth of the bacterium Cutibacterium acnes, which is present on the skin.[15] ... These gene candidates include certain variations in tumor necrosis factor-alpha (TNF-alpha), IL-1 alpha, and CYP1A1 genes, ...
Main article: Transforming growth factor beta. During mandible development, most of it is formed through intramembranous ... before any genetic or morphological criteria were put in place for bone marrow or connective tissues. Osteoprogenitor cells can ... These cellular units will then develop into skeletal and other tissues, such as cartilage, tendon, ligament and muscle tissue. ... The transcription factor Sox9 can be found in multiple sites in the body (pancreas, central nervous system, intestines) and it ...
Additional growth factors: Adrenomedullin. *Colony-stimulating factors (see here instead). *Connective tissue growth factor ( ... It is mainly used to treat cases of NSCLC that harbour mutations in the epidermal growth factor receptor (EGFR) gene.[5] ... Afatinib covalently binds to cysteine number 797 of the epidermal growth factor receptor (EGFR) via a Michael addition (IC50 = ... Phase II results for breast cancer that over-expresses the protein human epidermal growth factor receptor 2 (Her2-positive ...
Human brains are surrounded by a system of connective tissue membranes called meninges that separate the brain from the skull. ... Risk factors. Neurofibromatosis, exposure to vinyl chloride, Epstein-Barr virus, ionizing radiation[1][2][3]. ... uncontrolled mitosis (growth by division beyond the normal limits). *anaplasia: the cells in the neoplasm have an obviously ... in the sense that the neoplasm invades the space occupied by adjacent tissue, thereby pushing the other tissue aside and ...
... a major constituent of extracellular microfibrils which form connective tissues.[36] Over 1,000 different mutations in FBN1 ... Selectional pleiotropy occurs when the resulting phenotype has many effects on fitness (depending on factors such as age and ... This can cause symptoms such as mental retardation, abnormal gait and posture, and delayed growth. Because tyrosine is used by ... which consequently relates to connective tissues elongating progressively and weakening. Because these fibers are found in ...
Vitamin A assists in the maintenance and promotion of healthy growth of skin and tissues cells. Healthy growth of tissue cells ... teeth and connective tissues - Aids the production of collagen which is the foundational matrix of bones, teeth, tendons, ... study suggesting that good nutrition as a child decreases lung damage as an adult as well as having a large influencing factor ... Zinc ensure the body has healthy growth by influencing cell division and cell growth. Development of chronic disease has become ...
It also makes blood vessels more permeable so neutrophils and clotting proteins can get into connective tissue more easily. ... Cancer - either by growth factors secreted by the tumor or invasion of bone marrow by the cancer ... Monocytes migrate from the bloodstream to other tissues and differentiate into tissue resident macrophages, Kupffer cells in ... Some leucocytes migrate into the tissues of the body to take up a permanent residence at that location rather than remaining in ...
The biochemistry of Hunter syndrome is related to a problem in a part of the connective tissue of the body known as the ... The age at onset of symptoms and the presence/absence of behavioral disturbances are predictive factors of ultimate disease ... growth and development, communication within the body, and protection from infection. Another critical function is the ... As a result, GAGs build up in cells throughout the body, particularly in tissues that contain large amounts of dermatan sulfate ...
Additional growth factors: Adrenomedullin. *Colony-stimulating factors (see here instead). *Connective tissue growth factor ( ... BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. ... BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor. ... growth factor activity. • GO:0001948 protein binding. Cellular component. • cytoplasm. • perinuclear region of cytoplasm. • ...
The influence of isotretinoin and 5-a reductase inhibitors in metaloproteases of connective tissue in patients with ance] (in ... Risk factors for skeletal effects include older age, greater dosage and longer course of treatment. Most bone changes cause no ... Isotretinoin may stop long bone growth in young people who are still growing.[7] Premature epiphyseal closure can occur in ... While excessive bone growth has been raised a possible side effect, a 2006 review found little evidence for this.[84] ...
Mast cells are a type of innate immune cell in connective tissue and the mucous membranes. They are intimately associated with ... Chemical factors produced during inflammation attract phagocytes, especially neutrophils.[5] Neutrophils then trigger other ... Neutrophil granules contain a variety of toxic substances that kill or inhibit growth of bacteria and fungi. The main products ... It sets up a physical barrier against the spread of infection, and promotes healing of damaged tissue after the clearance of ...
Mast cells are a type of innate immune cell that reside in connective tissue and in the mucous membranes. They are intimately ... where they induce IFN production with the presence of a particular transcription factor and activate transcription factor 2. ... Neutrophil granules contain a variety of toxic substances that kill or inhibit growth of bacteria and fungi. Similar to ... swelling of affected tissues, such as the upper throat during the common cold or joints affected by rheumatoid arthritis; ...
Hence, knowledge is the catalyst and connective tissue in modern economies. With Earth's depleting natural resources, the need ... Social structures, cultural context and other factors influencing social relations are therefore of fundamental importance to ... "Growth & Innovation Policies For a Knowledge Economy. Experiences From Finland, Sweden & Singapore" (PDF). Working Paper 156 ... "The Global Innovation Index 2012: Stronger Innovation Linkages for Global Growth" (PDF). INSEAD. Archived from the original ...
Additional growth factors: Adrenomedullin. *Colony-stimulating factors (see here instead). *Connective tissue growth factor ( ... Pegaptanib is a pegylated anti-vascular endothelial growth factor (VEGF) aptamer, a single strand of nucleic acid that binds ... This then reduces the growth of the blood vessels located within the eye and works to control the leakage and swelling.[2] ... Hepatoma-derived growth factor (HDGF). *Interleukins/T-cell growth factors (see here instead) ...
adipose tissue. A type of loose connective tissue made of mostly adipocytes and found in human and animal tissue, where it is ... The collection of glands that produce hormones which regulate metabolism, growth and development, tissue function, and a wide ... A species' niche includes all of its interactions with the biotic and abiotic factors of its environment.. nucleic acid. The ... The fibrous connective tissue that connects bones to other bones and is also known as articular ligament, articular larua, ...
PDGF is a required element in cellular division for fibroblasts, a type of connective tissue cell that is especially prevalent ... Platelet-derived growth factor (PDGF) is one among numerous growth factors that regulate cell growth and division. In ... "Vascular endothelial growth factor B, a novel growth factor for endothelial cells". Proc. Natl. Acad. Sci. U.S.A. 93 (6): 2567- ... Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)[16][17] which are ...
They are often due to damage of blood vessels and connective tissue cells. Many late effects are reduced by fractionating ... Subsequent damage occurs from vascular constriction and nerve compression due to uncontrolled fibrous tissue growth caused by ... aggravating factors included.[19] The increase is dose dependent, related to the RT's dose strength, volume and location.. ... Ionizing radiation works by damaging the DNA of cancerous tissue leading to cellular death. To spare normal tissues (such as ...
The spermatic cord, formed from spermatic artery, vein and nerve bound together with connective tissue passes into the testis ... The gene for sexual differentiation in humans, called the testis determining factor (TDF),[13] is located on the short arm of ... Testosterone secretion by the interstitial cells of the testes then causes the growth and development of the mesonephric ducts ... Erection occurs because sinuses within the erectile tissue of the penis become filled with blood. The arteries of the penis are ...
This was the first time this abnormal disease affecting cartilage and connective tissue was observed and analysed. His ... He considered social factors such as poverty major causes of disease.[103] He even attacked Koch's and Ignaz Semmelweis' policy ... Virchow's Law, during craniosynostosis, skull growth is restricted to a plane perpendicular to the affected, prematurely fused ... present in mature tissue.[40] Virchow believed that cancer is caused by severe irritation in the tissues, and his theory came ...
Additional growth factors: Adrenomedullin. *Colony-stimulating factors (see here instead). *Connective tissue growth factor ( ... A major fetal growth factor in contrast to Insulin-like growth factor 1, which is a major growth factor in adults."[5] ... A major fetal growth factor in contrast to Insulin-like growth factor 1, which is a major growth factor in adults. ... It is believed to be a major fetal growth factor in contrast to Insulin-like growth factor 1, which is a major growth factor in ...
Additional growth factors: Adrenomedullin. *Colony-stimulating factors (see here instead). *Connective tissue growth factor ( ... BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. ... BDNF, brain-derived neurotrophic factor, ANON2, BULN2, Brain-derived neurotrophic factor, brain derived neurotrophic factor. ... growth factor activity. Cellular component. • cytoplasm. • perinuclear region of cytoplasm. • mitochondrion. • nuclear speck. • ...
Additional growth factors: Adrenomedullin. *Colony-stimulating factors (see here instead). *Connective tissue growth factor ( ... Hepatoma-derived growth factor (HDGF). *Interleukins/T-cell growth factors (see here instead) ...
In addition to SLE, these antibodies are highly associated with mixed connective tissue disease. Anti-nRNP antibodies recognise ... "A Serum Factor in Lupus Erythematosus with Affinity for Tissue Nuclei". BMJ. 2 (5047): 732-734. doi:10.1136/bmj.2.5047.732. PMC ... The sp100 antigen is found within nuclear bodies; large protein complexes in the nucleus that may have a role in cell growth ... A significant number of patients are diagnosed as systemic lupus erythematosus or undifferentiated connective tissue disease ...
Skeletal muscles are sheathed by a tough layer of connective tissue called the epimysium. The epimysium anchors muscle tissue ... and a decrease in sensitivity to or the availability of critical secreted growth factors that are necessary to maintain muscle ... Muscle tissue is a soft tissue, and is one of the four fundamental types of tissue present in animals. There are three types of ... Exercise has several effects upon muscles, connective tissue, bone, and the nerves that stimulate the muscles. One such effect ...
Most structural proteins are fibrous proteins; for example, collagen and elastin are critical components of connective tissue ... Kauzmann W (May 1956). "Structural factors in protein denaturation". Journal of Cellular Physiology. 47 (Suppl 1): 113-31. doi: ... In animals such as dogs and cats, protein maintains the health and quality of the skin by promoting hair follicle growth and ... Fibrous proteins are often structural, such as collagen, the major component of connective tissue, or keratin, the protein ...
Additional growth factors: Adrenomedullin. *Colony-stimulating factors (see here instead). *Connective tissue growth factor ( ... Growth factors. *Epidermal growth factor. *Fibroblast growth factor. *Nerve growth factor. *Platelet-derived growth factor ... Hepatoma-derived growth factor (HDGF). *Interleukins/T-cell growth factors (see here instead) ...
D, subepithelial connective tissue. E, oral epithelium. F, free gingival margin. G, gingival sulcus. H, principal gingival ... This can be the result of factors such as: thickness and degree of keratinization of the epithelium, blood flow to the gums, ... The gingival cavity microecosystem, fueled by food residues and saliva, can support the growth of many microorganisms, of which ... When gum tissue is not healthy, it can provide a gateway for periodontal disease to advance into the deeper tissue of the ...
The soft tissues and connective fibres that cover and protect the underlying cementum, periodontal ligament and alveolar bone ... Risk factors are variables that contribute to disease, rather than being factors that induce disease. Risk factors may be seen ... Multiplication: Through continued growth and maturation of existing plaque micro-organisms and the further recruitment of later ... Risk factors[edit]. A risk factor is a variable that in health, can be defined as "a characteristic associated with an ...
... development by modulating the local expression in breast tissue of an assortment of autocrine and paracrine growth factors,[25] ... connective) tissue, while IGF-1, in contrast, has been found to not do this.[12][13] In addition to estrogen and GH/IGF-1 both ... tumor necrosis factor β (TNF-β), transforming growth factor α (TGF-α),[67] transforming growth factor β (TGF-β),[68] heregulin, ... epidermal growth factor (EGF), IGF-1, and fibroblast growth factor (FGF), which in turn have specific roles in breast growth ...
Duncan, Matthew R., et al., "Connective Tissue Growth Factor Mediates Transforming Growth Factor β-Induced Collagen Synthesis: ... "Connective tissue growth factor" or "CTGF" refers to the amino acid sequences of substantially purified CTGF derived from any ... Monoclonal antibody against connective tissue growth factor and medicinal uses thereof. 2003-05-13. Tamatani et al.. ... Connective Tissue Growth Factor (CTGF). CTGF is a 36 kD, cysteine-rich, heparin binding, secreted glycoprotein originally ...
Background: Connective tissue growth factor (CTGF), which is regulated by transforming growth factor beta (TGF-beta), has ... Connective tissue growth factor is a regulator for fibrosis in human chronic pancreatitis Ann Surg. 1999 Jul;230(1):63-71. doi ... Tissue samples of 25 previously healthy organ donors (12 women, 13 men) served as controls. The expression of TGF-beta1, TbetaR ... In addition, TbetaR-I(ALK5) mRNA was increased in 50% of CP tissue samples (1.8-fold). By in situ hybridization, TGF-beta1, ...
Growth Factors and Connective Tissue Homeostasis in Periodontal Disease , IntechOpen, Published on: 2012-01-20. Authors: ... Growth Factors and Connective Tissue Homeostasis in Periodontal Disease. By Catalina Pisoschi, Camelia Stanciulescu and Monica ...
Accumulating evidence suggests that connective tissue growth factor (CCN2) plays a central role in fibrotic conditions in many ... Study suggests that connective tissue growth factor plays a vital role in liver fibrosis progression. *Download PDF Copy ... Accumulating evidence suggests that connective tissue growth factor (CCN2) plays a central role in fibrotic conditions in many ... Growth Factor, Hepatitis A, Hepatitis B, Hepatitis C, Hepatitis D, Hepatitis E, Hepatology, Hospital, in vitro, Liver, Liver ...
... connective tissue growth factor include Culturing and Measuring Fetal and Newborn Murine Long Bones, Quantitative Micro-CT ... Connective Tissue Growth Factor: A Ccn protein family member that regulates a variety of extracellular functions including Cell ...
Prognosis Cholecystitis Gallbladder cancer Genetic aspects Research Gene expression Physiological aspects Growth factors ... Connective tissue growth factor immunohistochemical expression is associated with gallbladder cancer progression.(Report) by ... tissue+growth+factor+immunohistochemical+expression+is...-a0331079988. *APA style: Connective tissue growth factor ... Tumor necrosis factor alpha suppresses the induction of connective tissue growth factor by transforming growth factor-beta in ...
Compare connective tissue growth factor ELISA Kits from BioVendor Laboratory Medicine, Inc. from leading suppliers on ... connective tissue growth factor ELISA Kits from BioVendor Laboratory Medicine, Inc.. Clear ... connective tissue growth factor ELISA Kits from BioVendor Laboratory Medicine, Inc.. The ELISA (enzyme-linked immunosorbent ... Your search returned 1 connective tissue growth factor ELISA ELISA Kit across 1 supplier. ...
Connective Tissue Growth Factor is a part of the CCN family of proteins. The CCN family presently consists of six members in ... Module I shares partial identity with the N-terminal part of the Insulin-like Growth Factor Binding Proteins (IGFBPs).. Module ... Downregulation of CCN2 expression in these cells was associated with a growth arrest at the G1/S transition while over- ... II includes a stretch of 70amino acid residues - which shares sequence identity with the Von Willebrand Factor Type C repeat ( ...
connective tissue growth factor;. rhCTGF,. recombinant human CTGF;. PDGF,. platelet-derived growth factor;. [QAYLL]IGF-II,. [ ... but not other growth factors, including PDGF, epidermal growth factor, and basic fibroblast growth factor (15). The CTGF gene ... A closely related family of genes encoding connective tissue growth factor (CTGF) (10), the nov oncogene (11), and cyr61 (12) ... The closely related mammalian genes connective tissue growth factor (CTGF) gene, nov, and cyr61 encode secreted proteins that ...
Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats. ... Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats. J. Gene Med., 8: 889-900. doi: 10.1002/ ...
Polymer-tethered epidermal growth factor as an inductive biomaterial surface for connective tissue progenitors. Download ... Polymer-tethered epidermal growth factor as an inductive biomaterial surface for connective tissue progenitors. Research and ... Connective tissue progenitors (CTP) can act as a pluripotent source of reparative cells during injury and therefore have great ... However, the response of CTP to most growth factors and cytokines is unknown. Many envisioned applications of CTP, such as ...
Connective tissue growth factor: a mediator of TGF-β action on fibroblasts. Cytokine Growth Factor Rev 1997; 8: 171-9. ... Connective tissue growth factor mediates transforming growth factor β-induced collagen synthesis: down-regulation by cAMP. ... Vascular endothelial growth factor induces expression of connective tissue growth factor via KDR, Flt1, and ... Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor. ...
Showing Protein Connective tissue growth factor (HMDBP02161). IdentificationBiological propertiesGene propertiesProtein ... Involved in insulin-like growth factor binding. Specific Function. Major connective tissue mitoattractant secreted by vascular ... Ball DK, Rachfal AW, Kemper SA, Brigstock DR: The heparin-binding 10 kDa fragment of connective tissue growth factor (CTGF) ... Bradham DM, Igarashi A, Potter RL, Grotendorst GR: Connective tissue growth factor: a cysteine-rich mitogen secreted by human ...
Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role ... Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role ...
F. R. Henshaw, P. Boughton, L. Lo, S. V. McLennan, and S. M. Twigg, "Topically Applied Connective Tissue Growth Factor/CCN2 ... Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: Potential Role ... Comment on "Topically Applied Connective Tissue Growth Factor/CCN2 Improves Diabetic Preclinical Cutaneous Wound Healing: ...
The Functional and Clinicopathological Roles and Therapeutic Implication of Connective Tissue Growth Factor in Peritoneal ... The Functional and Clinicopathological Roles and Therapeutic Implication of Connective Tissue Growth Factor in Peritoneal ... Cancer tissue and adjacent normal tissue Urine Blood serum and blood plasma ... It is a multifunctional growth factor involved in wound healing, inflammation, cell adhesion, chemotaxis, apoptosis, tumor ...
OBJECTIVE Connective tissue growth factor (CTGF), also known as CCN2, is a potent chemotactic and extracellular matrix-inducing ... Plasma Connective Tissue Growth Factor (CTGF/CCN2) Levels Predict Myocardial Infarction in the Veterans Affairs Diabetes Trial ... Plasma Connective Tissue Growth Factor (CTGF/CCN2) Levels Predict Myocardial Infarction in the Veterans Affairs Diabetes Trial ... Plasma Connective Tissue Growth Factor (CTGF/CCN2) Levels Predict Myocardial Infarction in the Veterans Affairs Diabetes Trial ...
Connective Tissue Growth Factor-Specific Monoclonal Antibody Therapy Inhibits Pancreatic Tumor Growth and Metastasis. Nadja ... Connective Tissue Growth Factor-Specific Monoclonal Antibody Therapy Inhibits Pancreatic Tumor Growth and Metastasis ... Connective Tissue Growth Factor-Specific Monoclonal Antibody Therapy Inhibits Pancreatic Tumor Growth and Metastasis ... Connective Tissue Growth Factor-Specific Monoclonal Antibody Therapy Inhibits Pancreatic Tumor Growth and Metastasis ...
Connective tissue growth factor: structure-function relationships of a mosaic, multifunctional protein. Growth Factors 2008;26: ... Regulation of pancreatic function by connective tissue growth factor (CTGF, CCN2). Cytokine Growth Factor Rev 2013;24:59-68pmid ... Connective tissue growth factor gene regulation. Requirements for its induction by transforming growth factor-beta 2 in ... The β-cell proliferative factor connective tissue growth factor (CTGF/CCN2) is a member of the CCN family of secreted ...
Connective tissue growth factor (CTGF) is a secreted protein that is strongly induced in human and experimental heart failure. ... Connective tissue growth factor overexpression in cardiomyocytes promotes cardiac hypertrophy and protection against pressure ... Surprisingly, cardiac tissues of both models did not show increased fibrosis or enhanced gene expression of fibrotic markers. ...
Regulation and function of connective tissue growth factor/CCN2 in tissue repair, scarring and fibrosis. Cytokine Growth Factor ... Connective tissue growth factor (CTGF) is a secreted protein that has been identified as a powerful inducer of ECM synthesis.3- ... Connective tissue growth factor (CTGF) is a key molecule in the process of fibrosis and therefore seems an attractive ... miR-133 and miR-30 Regulate Connective Tissue Growth Factor. Rudy F. Duisters, Anke J. Tijsen, Blanche Schroen, Joost J. ...
Exosomes mediate intercellular transfer of pro-fibrogenic connective tissue growth factor (CCN2) between hepatic stellate cells ... which produce and respond to fibrotic mediators such as connective tissue growth factor (CCN2). The aim of this study was to ...
... is known to be a multifunctional growth factor that is overexpressed in several types of malignancies. In this study, effects ... Connective tissue growth factor (CTGF) is known to be a multifunctional growth factor that is overexpressed in several types of ... Suppressive effect of overexpressed connective tissue growth factor on tumor cell growth in a human oral squamous cell ... Surprisingly, our results indicated that CTGF-overexpressed clones were characterized by attenuated cell growth and less potent ...
Connective Tissue Growth Factor. By Technical Data. Connective Tissue Growth Factor - Proteins. Connective Tissue Growth Factor ... Connective Tissue Growth Factor - Proteins. Connective Tissue Growth Factor - for Western blotting - Proteins. Connective ... References to Connective Tissue Growth Factor. *Abdel-Wahab N, Weston BS, Roberts T, Mason RM. Connective tissue growth factor ... Connective Tissue Growth Factor - for Cell culture and/or animal studies - Proteins. Connective Tissue Growth Factor - from ...
Connective Tissue Growth Factor. By Technical Data. Connective Tissue Growth Factor - Proteins. Connective Tissue Growth Factor ... Connective Tissue Growth Factor - Proteins. Connective Tissue Growth Factor - for Western blotting - Proteins. Connective ... References to Connective Tissue Growth Factor. *Abdel-Wahab N, Weston BS, Roberts T, Mason RM. Connective tissue growth factor ... Connective Tissue Growth Factor - tag: His - Proteins. Connective Tissue Growth Factor - from E. coli - Proteins. Recombinant ...
Connective tissue growth factor: a mediator of TGF-beta action on fibroblasts. Cytokine Growth Factor Rev. 1997; 8:171-9. [PMID ... Connective tissue growth factor in pterygium: simultaneous presence with vascular endothelial growth factor - possible ... The connective tissue growth factor (CTGF) belongs to the CCN (cyr61, ctgf, nov) protein family. The known six members of this ... Connective-tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-beta. Nat Cell Biol. 2002; 4:599-604. [PMID: ...
To investigate the effects of Connective Tissue Growth Factor Antisense Oligonuclcotide (CTGF ASON) and Transforming Growth ... F. Kang, W. Chen, X. Wang; Effects of Connective Tissue Growth Factor Antisense on Rabbit Cornea Wound Repair. Invest. ... Effects of Connective Tissue Growth Factor Antisense on Rabbit Cornea Wound Repair ... Effects of Connective Tissue Growth Factor Antisense on Rabbit Cornea Wound Repair ...
Connective tissue growth factor (CTGF) plays an important role in the pathogenesis of chronic fibrotic diseases. However, the ... Connective Tissue Growth Factor causes EMT-like cell fate changes in vivo and in vitro ... Connective Tissue Growth Factor causes EMT-like cell fate changes in vivo and in vitro ... Connective Tissue Growth Factor causes EMT-like cell fate changes in vivo and in vitro ...
Transforming growth factor beta induces anchorage-independent growth of NRK fibroblasts via a connective tissue growth factor- ... Connective tissue growth factor: a mediator of TGF-beta action on fibroblasts (review). Cytokine Growth Factor Rev. 1997;8:171- ... Connective tissue growth factor gene regulation: requirements for its induction by transforming growth factor-beta 2 in ... Connective tissue growth factor mediates transforming growth factor beta-induced collagen synthesis: down-regulation by cAMP. ...
  • Characterization of Cell-Associated and Soluble Forms of Connective Tissue Growth Factor (CTGF) Produced by Fibroblast Cells in Vitro," Growth Factor (1998) 15:199-213. (freepatentsonline.com)
  • Connective tissue growth factor (CTGF), which is regulated by transforming growth factor beta (TGF-beta), has recently been implicated in skin fibrosis and atherosclerosis. (nih.gov)
  • In the present study, the authors analyzed the concomitant presence of TGF-beta1 and its signaling receptors-TGF-beta receptor I, subtype ALK5 (TbetaR-I(ALK5)), and TGF-beta receptor II (TbetaR-II)-as well as CTGF and collagen type I in the pancreatic tissue of patients undergoing surgery for chronic pancreatitis. (nih.gov)
  • Northern blot analysis showed that CP tissue samples exhibited concomitant enhanced mRNA expression of TGF-beta1 (38-fold), TbetaR-II (5-fold), CTGF (25-fold), and collagen type I (24-fold) compared with normal controls. (nih.gov)
  • Moreover, CTGF mRNA expression levels correlated positively with the degree of fibrosis in CP tissues. (nih.gov)
  • Connective tissue growth factor (CTGF), also known as CCN2, is a member of the CCN family. (thefreelibrary.com)
  • For example, high CTGF levels have been associated with tumor growth and adverse prognosis in pancreatic cancer (12) and gastric cancer. (thefreelibrary.com)
  • The closely related mammalian genes connective tissue growth factor (CTGF) gene, nov , and cyr61 encode secreted proteins that also contain the conserved sequences and IGFBP motifs in their NH 2 termini. (pnas.org)
  • CTGF has been identified as a major chemotactic and mitogenic factor for connective tissue cells ( 10 ). (pnas.org)
  • CTGF gene expression is increased in human foreskin fibroblasts after activation with transforming growth factor β (TGF-β), but not other growth factors, including PDGF, epidermal growth factor, and basic fibroblast growth factor ( 15 ). (pnas.org)
  • Differential cDNA microarray analyses showed that connective tissue growth factor (CTGF) was expressed at low levels in nontumor-promoting prostate stromal cells and was constitutively expressed in tumor-promoting prostate stromal cells. (aacrjournals.org)
  • Expression of CTGF in tumor-reactive stroma induced significant increases in microvessel density and xenograft tumor growth under several conditions tested. (aacrjournals.org)
  • Connective tissue growth factor (CTGF) has emerged as a potent mediator of TGF-β1 action in wound repair stromal responses and in fibrosis disorders ( 4 - 6 ). (aacrjournals.org)
  • CTGF is expressed by several stromal cell types, including endothelial cells, fibroblasts, smooth muscle cells, and myofibroblasts, and some epithelial cell types in diverse tissues. (aacrjournals.org)
  • Consistent with its role in connective tissue biology, CTGF enhances stromal extracellular matrix synthesis ( 16 ) and stimulates proliferation, cell adhesion, cell spreading, and chemotaxis of fibroblasts ( 10 , 16 , 21 ). (aacrjournals.org)
  • Ball DK, Rachfal AW, Kemper SA, Brigstock DR: The heparin-binding 10 kDa fragment of connective tissue growth factor (CTGF) containing module 4 alone stimulates cell adhesion. (hmdb.ca)
  • Recent studies showed that overexpression of CTGF in human oral squamous cell carcinoma reduces cell growth and tumorigenecity. (clinicaltrials.gov)
  • Similar tumor growth inhibitory effects were observed in lung cancer cells in which CTGF overexpression was less angiogenic and metastatic due to blocking of the VEGF A signaling pathway. (clinicaltrials.gov)
  • CTGF was also reported to be a key regulator of colorectal cancer invasion and metastasis, and it appears to be a better prognostic factor. (clinicaltrials.gov)
  • In order to answer these important questions, first, we have performed the preliminary studies to prove CTGF did express in different gastric cancer cell lines including AGS, N87, TSGH, and MKN-45 by using RT-PCR and Western blotting, and gastric cancer tissues by using immunohistochemical method. (clinicaltrials.gov)
  • OBJECTIVE Connective tissue growth factor (CTGF), also known as CCN2, is a potent chemotactic and extracellular matrix-inducing matricellular protein that has been implicated in progression of inflammatory and fibroproliferative disorders. (diabetesjournals.org)
  • An emerging role of CTGF/CCN2 is that of a prosclerotic factor implicated in the development of cardiac disease. (diabetesjournals.org)
  • Past studies have shown that connective tissue growth factor (CTGF) expression is elevated in human pancreatic adenocarcinomas and some pancreatic cancer cell lines. (aacrjournals.org)
  • To address whether and how CTGF influences tumor growth, we generated pancreatic tumor cell lines that overexpress different levels of human CTGF. (aacrjournals.org)
  • Although there was no effect of CTGF expression on proliferation in two-dimensional cultures, anchorage-independent growth (AIG) was enhanced. (aacrjournals.org)
  • The capacity of CTGF to enhance AIG in vitro was linked to enhanced pancreatic tumor growth in vivo when these cells were implanted s.c. in nude mice. (aacrjournals.org)
  • Consistent with this observation, anti-CTGF treatment of mice bearing established CTGF-expressing tumors abrogated CTGF-dependent tumor growth and inhibited lymph node metastases without any toxicity observed in normal tissue. (aacrjournals.org)
  • Together, these studies implicate CTGF as a new target in pancreatic cancer and suggest that inhibition of CTGF with a human monoclonal antibody may control primary and metastatic tumor growth. (aacrjournals.org)
  • In mice, connective tissue growth factor (CTGF) is expressed in embryonic β-cells and in adult β-cells during periods of expansion. (diabetesjournals.org)
  • CTGF treatment upregulates positive cell-cycle regulators and factors involved in β-cell proliferation, including hepatocyte growth factor, serotonin synthesis, and integrin β1. (diabetesjournals.org)
  • The β-cell proliferative factor connective tissue growth factor (CTGF/CCN2) is a member of the CCN family of secreted extracellular matrix-associated proteins ( 7 ). (diabetesjournals.org)
  • Depending on the growth factor milieu in the microenvironment, CTGF can regulate several cellular processes including proliferation, adhesion, extracellular matrix remodeling, and angiogenesis ( 12 ). (diabetesjournals.org)
  • Connective tissue growth factor (CTGF) is a secreted protein that is strongly induced in human and experimental heart failure. (mdc-berlin.de)
  • Connective tissue growth factor (CTGF) is a key molecule in the process of fibrosis and therefore seems an attractive therapeutic target. (ahajournals.org)
  • Connective tissue growth factor (CTGF) is a secreted protein that has been identified as a powerful inducer of ECM synthesis. (ahajournals.org)
  • 3-5 The importance of CTGF is recognized in many different forms of pathology and has been described in different organs, including the heart, to be an important mediator of tissue fibrosis. (ahajournals.org)
  • CTGF expression is induced by transforming growth factor (TGF)β and other prohypertrophic stimuli such as endothelin. (ahajournals.org)
  • 3,6,10 It has been traditionally assumed that growth factors such as TGFβ and endothelin regulate CTGF expression exclusively in fibroblasts. (ahajournals.org)
  • Connective tissue growth factor (CTGF) is known to be a multifunctional growth factor that is overexpressed in several types of malignancies. (nih.gov)
  • Surprisingly, our results indicated that CTGF-overexpressed clones were characterized by attenuated cell growth and less potent tumorigenicity, with coincidental downregulation of prothymosin alpha gene. (nih.gov)
  • Although CTGF is known to promote cell proliferation in mesenchymal cells, our present results suggest that CTGF acts as a negative regulator of the cell growth in oral squamous cell carcinoma possibly through its interaction with growth modifiers inside the cell. (nih.gov)
  • The CCN family presently consists of six members in human also known as: Cyr61 (Cystein rich 61), CTGF (Connective Tissue Growth Factor), Nov (Nephroblastoma Overexpressed gene), WISP-1, 2 and 3 (Wnt-1 Induced Secreted Proteins). (biovendor.com)
  • The results suggest that CTGF potentially acts as a modulating factor in inflammatory and wound healing response in fibroblasts of the human eye. (molvis.org)
  • The connective tissue growth factor (CTGF) belongs to the CCN (cyr61, ctgf, nov) protein family. (molvis.org)
  • To investigate the effects of Connective Tissue Growth Factor Antisense Oligonuclcotide (CTGF ASON) and Transforming Growth Factor-β 2 Antisense Oligonuclcotide (TGF-β 2 ASON) on the wound rabbit cornea healing, we determined whether Connective Tissue Growth Factor reducing cornea scarring through TGF-β 2 stimulation. (arvojournals.org)
  • Connective tissue growth factor (CTGF) plays an important role in the pathogenesis of chronic fibrotic diseases. (biologists.org)
  • Lung fibrosis was associated with a marked increase in cells co-expressing epithelial and mesenchymal markers in the lesional and unaffected lung tissue of Col1a2-CTGF mice. (biologists.org)
  • Connective tissue growth factor (CTGF) appears to play a significant role in mediating fibrosis in several tissues. (arvojournals.org)
  • Three experiments were performed: (1) CTGF and transforming growth factor (TGF)-β expression were measured quantitatively after GFS, using ELISA. (arvojournals.org)
  • 2) After GFS conjunctival bleb tissues were immunostained for the presence of CTGF and TGF-β. (arvojournals.org)
  • CTGF and TGF-β were expressed maximally by day 5 after surgery and were both shown to be present in the bleb tissues after GFS. (arvojournals.org)
  • Connective tissue growth factor (CTGF) is a secreted peptide that was originally discovered in human umbilical vein endothelial cell conditioned medium and has been implicated in multiple cellular events, including angiogenesis, skeletogenesis, and wound healing. (arvojournals.org)
  • 23 24 25 The actions of CTGF have been clearly distinguished from those of TGF-β by showing that CTGF alone does not induce anchorage-independent growth of fibroblasts. (arvojournals.org)
  • 27 The mechanisms of CTGF induction involve Smads, Ras/MEK/ERK, protein kinase C, and fibroblast-enriched factors. (arvojournals.org)
  • Connective tissue growth factor (CTGF/CCN2) is a member of the CCN family of matricellular proteins. (ovid.com)
  • CTGF can bind to several other growth factors modifying their function. (ovid.com)
  • Connective tissue growth factor (CTGF) is implicated as a factor promoting tissue fibrosis in several disorders, including diabetic nephropathy. (asnjournals.org)
  • K252a, a known selective inhibitor of Trk, blocked this phosphorylation, CTGF-induced activation of signaling proteins, and CTGF-dependent induction of the transcription factor TGF-β-inducible early gene in HMC. (asnjournals.org)
  • The presence of multiple domains in CTGF suggests a role for the growth factor as an integrator of several other signaling molecules such as growth factors, integrins, and extracellular matrix proteins. (asnjournals.org)
  • In conclusion CTGF supports very efficiently survival of certain cells without stimulating their cell growth. (uzh.ch)
  • Background and objectives Connective Tissue Growth Factor (CTGF/CCN-2) is a key player in fibrosis. (asnjournals.org)
  • Connective-tissue growth factor (CTGF/CCN-2) is a main mediator of fibrogenesis both downstream and independent of transforming growth factor-β1 ( 1 - 3 ). (asnjournals.org)
  • Cooper, M. 2004-06-01 00:00:00 Diabetologia (2004) 47:965-968 DOI 10.1007/s00125-004-1423-6 Commentary The time has come to target connective tissue growth factor in diabetic complications Dysregulation of growth factors is amongst the chang- leading to the hypothesis that CTGF contributes to es that occur in cells and tissues in diabetes. (deepdyve.com)
  • Indeed, we have recently shown sequently, identifying the key growth factor(s) that that CTGF is also increased in non-renal tissue. (deepdyve.com)
  • The cause a particular type and stage of diabetic tissue increases in CTGF correspond with pathological in- damage is of potential importance in preventing and creases in myocardial type III collagen in rodent dia- betic cardiomyopathy [11]. (deepdyve.com)
  • In this context, connective tissue growth factor cient mouse model of atheroma, CTGF mRNA and (CTGF), also known as CCN2 [2], is a prime candi- protein levels are increased in the complex lesions at date. (deepdyve.com)
  • Fibroblast expression of one of these genes, connective tissue growth factor (CTGF), was confirmed by immunohistochemistry. (garvan.org.au)
  • Connective tissue growth factor (CTGF) is a peptide secreted by cultured endothelial cells and fibroblasts when stimulated by transforming growth factor-β (TGF-β), and is overexpressed during fibrotic processes in coronary arteries and in skin. (asnjournals.org)
  • Exposure of MC to TGF-β, increased glucose concentrations, or cyclic mechanical strain, all causal factors in diabetic glomerulosclerosis, markedly induced the expression of CTGF transcripts, while recombinant human CTGF was able to autoinduce its own expression. (asnjournals.org)
  • These results suggest that CTGF upregulation is an important factor in the pathogenesis of mesangial matrix accumulation and progressive glomerulosclerosis, acting downstream of TGF-β. (asnjournals.org)
  • CTGF protein content of LV myocardiac tissue was evaluated by quantitative immunohistochemistry staining. (cnki.com.cn)
  • CTGF mRNA level and hydroxyproline concentration in myocardiac tissue were measured.Result:In comparison with group C, Pa, Pp, and M groups had higher LV weight to body weight ratio, CVF, hydroxyproline concentration and CTGF levels in myocardiac tissue(P0.01). (cnki.com.cn)
  • But among the 3 ISO-treated groups, all of the parameters are significantly lower in Pa and Pp groups than in group M, especially in group Pa.Conclusion:These results suggest that peurarin can decrease the excessive collagen deposition induced by ISO in rat especially in early treatment, mostly through suppressing the overexpression of CTGF in myocardial tissue. (cnki.com.cn)
  • Connective tissue growth factor (CCN2, also termed CTGF) is one of six structurally related molecules that comprise the CCN family. (bmj.com)
  • Treatment of C57BL/6 mice with ethanol and cerulein resulted in increased staining for acinar interleukin-1 β (IL-1 β ), chemokine (C-C motif) ligand 3 (CCL3), or connective tissue growth factor (CTGF/CCN2) by Day 16 and this was associated with increased infiltration of F4/80-positive macrophages and increased expression of pancreatic CTGF/CCN2 mRNA. (gallusimmunotech.com)
  • To investigate the expression and the effect of connective tissue growth factor (CTGF) on rheumatic myocardial fibrosis of rheumatic heart disease (RHD). (onlinejacc.org)
  • CTGF is a heparin-binding multi-domain growth factor, stimulated by TGF- beta, and implicated as a paracrine regulator of mitosis, angiogenesis, cellular taxis and remodelling of the extracellular matrix in diverse epithelial cell layers. (endocrine-abstracts.org)
  • TGF-beta, GDF-9 and activin up-regulate CTGF mRNA in vitro , suggesting a paracrine/autocrine involvement of these growth/differentiation factors in regulating CTGF in the ovary. (endocrine-abstracts.org)
  • It was reported that the gene expression of ErbB4 receptor was up regulated by addition of connective tissue growth factor(CTGF) under the cultivation of a chondrocytic cell line HCS2/8. (nii.ac.jp)
  • Connective tissue growth factor (CTGF) is a protein which helps to make connective tissue, which connects, supports, binds, or separates other tissues or organs in the body. (isrctn.com)
  • The serum level of connective tissue growth factor (CTGF) and Creatine kinase-MB (CKMB) maximal value. (isrctn.com)
  • MBS040415 is a ready-to-use microwell, strip plate Sandwich ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Connective Tissue Growth Factor (CTGF) ELISA Kit target analytes in biological samples. (mybiosource.com)
  • In this thesis, we focus on the role of the antifibrotic bone morphogenetic proteins (BMPs, particularly BMP-6) and the profibrotic connective tissue growth factor (CTGF) in renal fibrogenesis, via experimental and human studies. (uu.nl)
  • Connective tissue growth factor (CCN2) is a 38 kDa cystein-rich secreted immediate early gene product and is a member of the CCN (Cyr61/CEF-10, CTGF/Fisp-12 and Nov) family of matricellular proteins. (biomedcentral.com)
  • Multiple factors have been implicated in the onset and progression of CR, including TGF beta, IL-6 and connective tissue growth factor (CTGF). (diff.org)
  • While associated with CR, the role of CTGF in CR and the factors necessary for CTGF induction in vivo are not understood. (diff.org)
  • Transduction of allografts with CTGF significantly increased fibrotic tissue development, though not to levels observed with TGF beta transduction. (diff.org)
  • This assay has high sensitivity and excellent specificity for detection of Connective Tissue Growth Factor (CTGF). (cloud-clone.com)
  • No significant cross-reactivity or interference between Connective Tissue Growth Factor (CTGF) and analogues was observed. (cloud-clone.com)
  • Matrices listed below were spiked with certain level of recombinant Connective Tissue Growth Factor (CTGF) and the recovery rates were calculated by comparing the measured value to the expected amount of Connective Tissue Growth Factor (CTGF) in samples. (cloud-clone.com)
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested 20 times on one plate, respectively. (cloud-clone.com)
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connective Tissue Growth Factor (CTGF) were tested on 3 different plates, 8 replicates in each plate. (cloud-clone.com)
  • The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Connective Tissue Growth Factor (CTGF) and their serial dilutions. (cloud-clone.com)
  • Connective tissue growth factor (CTGF/CCN2): A protagonist in cardiac allograft vasculopathy development? (cloud-clone.com)
  • During the course of our studies on mechanisms whereby TGFb stimulates connective tissue formation, we discovered another growth factor, Connective Tissue Growth Factor (CTG F). CTGF synthesis selectively induced in mesenchymal cells by TGFb. (grantome.com)
  • This suggests that CTGF may be an important therapeutic target for the control of connective tissue formation in fibrotic disorders. (grantome.com)
  • The current project will determine in a more detailed manner the role of the CTGF, CTG F, lGF and EGF receptors and the roles of EGF and IGF factor for signaling the mitogenic and matrigenic actions induced by TGFb in fibroblastic cells. (grantome.com)
  • Should the Bovine Connective Tissue Growth Factor (CTGF) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement. (signetlabs.com)
  • Description: A sandwich quantitative ELISA assay kit for detection of Bovine Connective Tissue Growth Factor (CTGF) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids. (signetlabs.com)
  • The loss of Connective tissue growth factor (Ctgf) does not affect islet physiology, indicating other existing problems in glucose homeostasis. (youngscientistjournal.org)
  • Connective tissue growth factor (CTGF) is a secreted extracellular matrix-associated protein, which play a role in regulating various cellular functions. (sparrho.com)
  • We found that connective tissue growth factor (Ctgf) was responsible for EleS-induced CSC survival and adhesion. (elsevier.com)
  • Connective Tissue Growth Factor (CTGF) expression in response to injury mediates matrix deposition in bleomycin-induced lung fibrosis. (ucl.ac.uk)
  • We therefore studied the link between a gene marker of ECM remodelling (connective tissue growth factor (CTGF)) and oestrogen biosynthesis (cytochrome P450 aromatase (P450 arom )) in rat granulosa cells. (bioscientifica.com)
  • CCN2, (connective tissue growth factor, CTGF) is a matricellular factor associated with fibrosis that plays an important role in the production and maintenance of fibrotic lesions. (qxmd.com)
  • Epithelial and connective tissue cell CTGF/CCN2 expression in gingival fibrosis. (qxmd.com)
  • Transforming growth factor-beta1 (TGFbeta1) stimulates connective tissue growth factor (CCN2/CTGF) expression in human gingival fibroblasts through a RhoA-independent, Rac1/Cdc42-dependent mechanism: statins with forskolin block TGFbeta1-induced CCN2/CTGF expression. (qxmd.com)
  • Tissue-specific mechanisms for CCN2/CTGF persistence in fibrotic gingiva: interactions between cAMP and MAPK signaling pathways, and prostaglandin E2-EP3 receptor mediated activation of the c-JUN N-terminal kinase. (qxmd.com)
  • Requirement for active glycogen synthase kinase-3β in TGF-β1 upregulation of connective tissue growth factor (CCN2/CTGF) levels in human gingival fibroblasts. (qxmd.com)
  • Connective Tissue Growth Factor (CTGF/CCN2) enhances lactogenic differentiation of mammary epithelial cells via integrin-mediated cell adhesion. (qxmd.com)
  • Effect of transforming growth factor-beta1 on expression of the connective tissue growth factor (CCN2/CTGF) gene in normal human gingival fibroblasts and periodontal ligament cells. (qxmd.com)
  • Background The pro-fibrogenic cytokine connective tissue growth factor (CTGF) plays an important role in the advancement and progression of fibrosis in many organ systems, including liver. (labsmart.net)
  • Research in many connective cells cell types possess demonstrated that CTGF works as a powerful downstream mediator of TGF-1, modulating its practical results [10]. (labsmart.net)
  • CTGF (Connective Tissue Growth Factor) is a Protein Coding gene. (genecards.org)
  • Recently, accumulated evidences support that connective tissue growth factor (CTGF) is implicated in tissue repair of many tissues. (elsevier.com)
  • Results: CTGF was expressed strongly in the endothelial cells migrating into the granulation tissue at the bottom of the sockets during 4 days after tooth extraction. (elsevier.com)
  • CTGF may play an important role in angiogenesis and granulation tissue formation specifically at early healing stage after tooth extraction to initiate alveolar bone repair. (elsevier.com)
  • The study was to evaluate the contribution of connective tissue growth factor (CTGF) to the regeneration of the torn anterior cruciate ligament (ACL) in a rabbit. (who.int)
  • The healing effect of the CTGF/FG-treated group was obviously better than that of the FG-treated group, with an increased amount of collagen fibers and fibroblasts in the ligament tissue. (who.int)
  • 2007). And nowadays, Connective tissue growth factor (CTGF) is a member of the improved living standards have spurred people to the C-terminal cysteine-rich protein (CCN) family (Wang participate in sporting events with greater enthusiasm, et al . (who.int)
  • 2013). However, is that tissue engineering which aimed to "replicate tissue whether CTGF is helpful in the repair of ACL injury has and organ", provides another innovative choice for the remained undetermined. (who.int)
  • 2012). and possible mechanism of CTGF in the treatment of Replacing natural tissue with appropriate cells, ACL injury. (who.int)
  • In the present study, we investigated the effects of connective tissue growth factor (CTGF) on the biology of cultured human TM cells. (qxmd.com)
  • Connective tissue growth factor (CTGF/CCN2) is a downstream mediator for TGF-beta1-induced extracellular matrix production in osteoblasts. (qxmd.com)
  • Connective tissue growth factor (CTGF) has been proposed to play a key role in the pathogenesis of hepatic fibrosis in biliary atresia (BA). (cdc.gov)
  • ET-1 further induced connective tissue growth factor (CTGF), as did TGF-β1, however, to lower levels. (wiley.com)
  • We report that connective tissue growth factor (Ctgf, also known as Ccn2) is highly expressed in murine bone marrow stromal cells. (edu.au)
  • The results showed that the activator protein-1 and nuclear factor κB binding sites of the CXCL8/IL-8 promoter are essential for CTGF-induced CXCL8/IL-8 transcription. (elsevier.com)
  • Connective tissue growth factor (cDNA FLJ77666, highly similar to Homo sapiens connective tissue growth factor (CTGF), mRNA) is proteolytically cut by matrix metallopeptidase-2 (M10.003) cleavage. (burnham.org)
  • Connective tissue growth factor (CTGF) overexpression underlies the development of pulmonary fibrosis. (elsevier.com)
  • Materials and Methods: To fibroblast differentiation 100 ng.mL -1 of connective tissue growth factor (CTGF), and for simulation microgravity, 2D clinostat was used. (ijbiotech.com)
  • Connective tissue growth factor (CTGF) is a secreted cytokine that plays a fundamental role in the development of tissue fibrosis by mediating many of the profibrotic effects of TGF-β. (warwick.ac.uk)
  • We report that both T. cruzi trypomastigotes and secreted parasite factor(s) antagonize TGF-β-dependent induction of CTGF in fibroblasts. (warwick.ac.uk)
  • Connective tissue growth factor (CTGF/CCN2) has strong inflammatory and profibrotic activities. (uu.nl)
  • Connective tissue growth factor (CTGF/CCN2) is axiomatically necessary for proper skeletal development and function . (temple.edu)
  • We found skeletal site-specific changes in growth plate organization , bone microarchitecture and shape and gene expression levels in CTGF KO compared to wild-type (WT) mice . (temple.edu)
  • Recently, connective tissue growth factor (CTGF) was demonstrated to be associated with aggressive characteristics, including proliferation, invasion and metastasis, in a number of malignancies. (spandidos-publications.com)
  • Connective Tissue Growth Factor (CCN Family Member 2 or Hypertrophic Chondrocyte Specific Protein 24 or Insulin Like Growth Factor Binding Protein 8 or IGFBP8 or CTGF) pipeline Target constitutes close to 11 molecules. (jsbmarketresearch.com)
  • The latest report Connective Tissue Growth Factor - Pipeline Review, H2 2018, outlays comprehensive information on the Connective Tissue Growth Factor (CCN Family Member 2 or Hypertrophic Chondrocyte Specific Protein 24 or Insulin Like Growth Factor Binding Protein 8 or IGFBP8 or CTGF) targeted therapeutics, complete with analysis by indications, stage of development, mechanism of action (MoA), route of administration (RoA) and molecule type. (jsbmarketresearch.com)
  • Connective Tissue Growth Factor (CCN Family Member 2 or Hypertrophic Chondrocyte Specific Protein 24 or Insulin Like Growth Factor Binding Protein 8 or IGFBP8 or CTGF) - Connective tissue growth factor (CTGF), also known as CCN2 is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins. (jsbmarketresearch.com)
  • Furthermore, this report also reviews key players involved in Connective Tissue Growth Factor (CCN Family Member 2 or Hypertrophic Chondrocyte Specific Protein 24 or Insulin Like Growth Factor Binding Protein 8 or IGFBP8 or CTGF) targeted therapeutics development with respective active and dormant or discontinued projects. (jsbmarketresearch.com)
  • Although connective tissue growth factor (CTGF) is known to promote cell proliferation , migration, adhesion, and extracellular matrix production in various tissues , studies on the role of CTGF in pulmonary hypertension have been limited. (bvsalud.org)
  • Here, we examined CTGF expression in the lung tissues of male Sprague Dawley rats treated with monocrotaline (MCT, 60 microgram/kg), a pneumotoxic agent known to induce PH in animals . (bvsalud.org)
  • RT-PCR analysis of the lung tissue at 5 weeks indicated significantly increased expression of CTGF in the MCT-treated group. (bvsalud.org)
  • Connective tissue growth factor (CTGF) is considered a cytokine mediating the effects of TGF-β. (biomedcentral.com)
  • In der vorliegenden Arbeit wurde untersucht, ob die Zytokin-vermittelte Signalkaskade, die letztendlich in einer manifesten Fibrose endet, mittels eines gegen den Connective Tissue Growth Faktor (CTGF) gerichteten Antikörpers (FG-3019) experimentell gehemmt werden kann und welche Wirkungen FG-3019 auf Zellen hat, die an fibrotischen Umbauprozessen beteiligt sind. (fu-berlin.de)
  • Als bedeutende und sich wechselseitig beeinflussende Faktoren im komplizierten Regelkreis der Fibroseentwicklung konnten CTGF und der Transforming Growth Faktorbeta (TGF-b) bestimmt werden. (fu-berlin.de)
  • Transforming growth factor (TGF)-β may play an important role in airway remodeling, and the fibrogenic effect of TGF-β may be mediated through connective tissue growth factor (CTGF) release. (edu.au)
  • The proinflammatory cytokines tumor necrosis factor-α and IL-1β reduced TGF-β1-stimulated mRNA expression of CTGF but did not inhibit TGF-β-induced Smad2/3 phosphorylation. (edu.au)
  • CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein). (wikipedia.org)
  • CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. (wikipedia.org)
  • In addition, CTGF also binds growth factors and extracellular matrix proteins. (wikipedia.org)
  • Accumulating evidence suggests that connective tissue growth factor (CCN2) plays a central role in fibrotic conditions in many organ systems. (news-medical.net)
  • A hallmark of fibrosing injury in the liver is the activation of hepatic stellate cells (HSCs) which become highly proliferative, synthesize increased levels of transforming growth factor (TGF)-β and CCN2, and produce excessive amounts of collagen. (news-medical.net)
  • Downregulation of CCN2 expression in these cells was associated with a growth arrest at the G1/S transition while over-expression of CCN2 induced a two-fold increase of the number of cells in the G1 phase. (prospecbio.com)
  • Exosomes mediate intercellular transfer of pro-fibrogenic connective tissue growth factor (CCN2) between hepatic stellate cells, the principal fibrotic cells in the liver. (sigmaaldrich.com)
  • Fibrogenic pathways in the liver are principally regulated by hepatic stellate cells (HSC), which produce and respond to fibrotic mediators such as connective tissue growth factor (CCN2). (sigmaaldrich.com)
  • Connective tissue growth factor(CCN2), a pathogenic factor in diabetic nephropathy. (ovid.com)
  • The distribution of LRP1 and its antagonist in the growth plate in vivo was consistent with that of CCN2 therein, which was produced by and transported from the chondrocytes in the prehypertrophic layer. (biologists.org)
  • Connective tissue growth factor (CCN2) is upregulated in pancreatic fibrosis and desmoplastic pancreatic tumours. (bmj.com)
  • Connective tissue growth factor (CCN2) is elevated in many fibrotic disorders. (biomedcentral.com)
  • It has platelet-derived growth factor (PDGF)-related biological and immunological activities, and it competes with PDGF for a cell-surface receptor ( 10 ). (pnas.org)
  • Acute imbalances in the expression, distribution, and/or biologic activities of cytokines such as transforming growth factor-β (TGF-β) and platelet-derived growth factor (PDGF) are an important part of the initial response to renal injury and are necessary for the increased collagen formation that is part of normal wound healing ( 1 , 2 ). (asnjournals.org)
  • The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. (genecards.org)
  • XXX: Isoelectric point microheterogeneity of CTAP-III, a human platelet-derived growth factor. (wizdom.ai)
  • The tissue distribution of the receptor for platelet-derived growth factor (PDGF) was investigated by immunohistochemistry on frozen sections from normal and inflamed synovial tissue using monoclonal antibodies to the receptor. (elsevier.com)
  • In this report, the global Connective Tissue Growth Factor market is valued at USD XX million in 2017 and is expected to reach USD XX million by the end of 2025, growing at a CAGR of XX% between 2017 and 2025. (marketdeeper.com)
  • Connective Tissue Growth Factor and IGF-1 are Produced by Human Renal Fibroblasts and Cooperate in the Induction of Collagen Production by High Glucose," Diabetes (2003) 52:2975-2983. (freepatentsonline.com)
  • The biologic relevance of human connective tissue growth factor (hCTGF) for primary human tenon fibroblasts (HTFs) was investigated by RNA expression profiling using affymetrix TM oligonucleotide array technology to identify genes that are regulated by hCTGF. (molvis.org)
  • After cloning and sequencing chicken connective tissue growth factor (cCTGF) we showed that in serum-deprived chicken embryo fibroblasts (CEF) the initial decline of cCTGF mRNA was followed by an increase. (uzh.ch)
  • Smad3 and extracellular signal-regulated kinase 1/2 coordinately mediate transforming growth factor-beta-induced expression of connective tissue growth factor in human fibroblasts. (qxmd.com)
  • Fibroblasts are omnipresent cells that constitute the stroma of almost all tissues (1-3). (ijbiotech.com)
  • A well-organized extracellular matrix (ECM) is necessary to maintain strength and functional integrity of cardiac tissue and is involved in communication between the different cells in the heart. (ahajournals.org)
  • The histopathological hallmark of chronic kidney disease is renal fibrosis, a process of extracellular matrix formation and atrophy of functional renal tissue resulting from sustained injury. (uu.nl)
  • Ovarian follicular development involves continual remodelling of the extracellular matrix (ECM) forming the basement membrane and intercellular framework that support granulosa cell (GC) growth and differentiation. (bioscientifica.com)
  • Transforming growth factor-beta 2 modulated extracellular matrix component expression in cultured human optic nerve head astrocytes. (qxmd.com)
  • Subepithelial fibrosis is caused by excessive deposition of connective tissue components and extracellular matrix (ECM) in the airway wall, which in turn results in lung function impairment [ 34 ]. (biomedcentral.com)
  • Using in vitro culture systems, we demonstrate that connective tissue growth factor potentiates B-cell proliferation and promotes pro-B to pre-B differentiation in the presence of interleukin-7. (edu.au)
  • Connective Tissue Growth Factor Mediates Transforming Growth Factor β-Induced Collagen Synthesis: Down-Regulation by cAMP," FASEB J. (1999) 13:1774-1786. (freepatentsonline.com)
  • These disorders are characterized by an overproduction of connective tissue (primarily collagen) and are widely recognized to be initiated in response to some type of injury which leads to the chronic inflammation and subsequent overproduction of growth factors that encourage the formation of connective tissue. (grantome.com)
  • Increased collagen deposition and accumulation is a common feature of fibrotic tissues. (qxmd.com)
  • Regulation of lysyl oxidase, collagen, and connective tissue growth factor by TGF-beta1 and detection in human gingiva. (qxmd.com)
  • Endothelin-1 (ET-1) plays an important role in tissue remodelling and fibrogenesis by inducing synthesis of collagen I via protein kinase C (PKC). (wiley.com)
  • In addition, elevated collagen level, transforming growth factor-β and connective tissue growth factor protein expression in conditioned medium were also found in hyperoxia LFs. (springer.com)
  • Mdx mice foci express elevated mRNAs levels of transforming growth factor type beta, collagen, fibronectin, the myofribroblast marker α-SMA, and the myogenic transcription factor myogenin. (uu.nl)
  • The full length protein consists of 4 modules: Module I shares partial identity with the N-terminal part of the Insulin-like Growth Factor Binding Proteins (IGFBPs). (prospecbio.com)
  • The CCN family members (excluding WISP2) share four conserved structural modules with sequence homologies similar to insulin-like growth factor-binding protein, von Willebrand factor, thrombospondin, and cysteine knot ( 8 ). (aacrjournals.org)
  • Martinerie C, Perbal B: Expression of a gene encoding a novel potential IGF binding protein in human tissues. (hmdb.ca)
  • The role of connective tissue growth factor, a multifunctional matricellular protein, in fibroblast biology. (qxmd.com)
  • Bone morphogenetic protein-7 is an antagonist of transforming growth factor-beta2 in human trabecular meshwork cells. (qxmd.com)
  • Gene and protein expression changes in human trabecular meshwork cells treated with transforming growth factor-beta. (qxmd.com)
  • Yes-associated protein (YAP) and transcriptional co-activator with PDZ-binding motif (TAZ) are the major downstream effectors of the Hippo pathway, which regulates tissue homeostasis, organ size, regeneration and tumorigenesis. (nih.gov)
  • In an ovalbumin-induced airway fibrosis model, the protein level of HDAC7 was increased in the lung tissue, and the distribution of HDAC7 was colocalized with α-SMA-positive cells in the subepithelial layer of the airway. (biomedcentral.com)
  • These domains are, from N- to C-termini, the insulin-like growth factor binding protein (IGFBP) domain, the von Willebrand type C repeats (vWC) domain, the thrombospondin type 1 repeat (TSR) domain, and a C-terminal domain (CT) with a cysteine knot motif. (wikipedia.org)
  • Connective tissue growth factor immunohistochemical expression is associated with gallbladder cancer progression. (thefreelibrary.com)
  • Surprisingly, cardiac tissues of both models did not show increased fibrosis or enhanced gene expression of fibrotic markers. (mdc-berlin.de)
  • Its expression is induced by a number of factors including TGF-β. (ovid.com)
  • Mice Lacking Connective Tissue Growth Factor in the Forebrain Exhibit Delayed Seizure Response, Reduced C-Fos Expression and Different Microglial Phenotype Following Acute PTZ Injection. (sparrho.com)
  • Lau LF, Nathans D (1987) Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c- fos or c- myc . (springer.com)
  • O'Brien TP, Yang GP, Sanders L, Lau LF (1990) Expression of cyr61 , a growth factor-inducible immediate-early gene. (springer.com)
  • Non-inflamed synovial tissue showed no staining, indicating that PDGF receptor expression is low or absent in normal tissue. (elsevier.com)
  • The acetylation of transcription factors mediates transcriptional activities and regulates gene expression [ 8 ]. (biomedcentral.com)
  • Astrogliosis and expression of transforming growth factor beta (TGF-β) are key components of scar formation and blood-brain barrier modulation. (biomedcentral.com)
  • Giordani VM, Debenedictus CM, Wang Y, Sanchez-Esteban J (2014) Epidermal growth factor receptor (EGFR) contributes to fetal lung fibroblast injury induced by mechanical stretch. (springer.com)
  • Tissue from synovitis with prominent proliferation of synovial lining showed intense staining for PDGF receptors in fibroblast-like cells of the lining and a less intense staining on vascular and connective tissue cells deeper in the stroma. (elsevier.com)
  • Two factors that appear responsible for the sustained overexpression of TGF-β are a high extracellular glucose concentration and altered glomerular hemodynamics. (asnjournals.org)
  • One common growth factor which has been demonstrated to be overproduced in all fibrotic disorders examined to date is TGFb, which is believed to be an initiator of the connective tissue formation in these disorders. (grantome.com)
  • However, the response of CTP to most growth factors and cytokines is unknown. (mit.edu)
  • ACL's self-healing ability is quite limited, due to the lack growth regulation and tissue modeling (Friedrichsen et of cytokines, growth factors and tissue repair cells al . (who.int)
  • Hematopoiesis occurs in a complex bone marrow microenvironment in which bone marrow stromal cells provide critical support to the process through direct cell contact and indirectly through the secretion of cytokines and growth factors. (edu.au)
  • Connective tissue progenitors (CTP) can act as a pluripotent source of reparative cells during injury and therefore have great potential in regenerative medicine and tissue engineering. (mit.edu)
  • Major connective tissue mitoattractant secreted by vascular endothelial cells. (hmdb.ca)
  • Bradham DM, Igarashi A, Potter RL, Grotendorst GR: Connective tissue growth factor: a cysteine-rich mitogen secreted by human vascular endothelial cells is related to the SRC-induced immediate early gene product CEF-10. (hmdb.ca)
  • In contrast, connective tissue growth factor is barely detectable in unfractionated adult bone marrow cells. (edu.au)
  • While connective tissue growth factor has been implicated in hematopoietic malignancies, and is known to play critical roles in skeletogenesis and regulation of bone marrow stromal cells, its role in hematopoiesis has not been described. (edu.au)
  • Using a chimeric fetal liver transplantation model, we show that absence of connective tissue growth factor has an impact on B-cell development, in particular from pro-B to more mature stages, which is linked to a requirement for connective tissue growth factor in bone marrow stromal cells. (edu.au)
  • 2014. Connective tissue growth factor is expressed in bone marrow stromal cells and promotes interleukin-7-dependent B lymphopoiesis. (edu.au)
  • In contrast, tissue from synovitis with prominent neovascularization showed a strong staining in the tunica media of the proliferating blood vessels as well as on connective tissue cells in the stroma. (elsevier.com)
  • Background: Mesenchymal stem cells (MSCs) are multipotent cells able to differentiating into a variety of mesenchymal tissues including osteoblasts, adipocytes and several other tissues. (ijbiotech.com)
  • They are adherent cells that proliferate in high rate and can be isolated from different adult tissues including adipose tissue (8). (ijbiotech.com)
  • Since adipose tissues are abundant and easy to access by less invasive methods, they are ideal sources of adult stem cells. (ijbiotech.com)
  • It persists for long periods and acts as barrier not only to axon regeneration but also to inflammatory cells in a manner that protects healthy tissue from nearby areas of intense inflammation [ 1 ]. (biomedcentral.com)
  • Connective tissue growth factor : a cysteine-rich mitogen secreted by human vascular endothelial cells by Douglass M. Bradham, Jr. (musc.edu)
  • Post a Comment for Connective tissue growth factor : a cysteine-rich mitogen secreted by human vascular endothelial cells by Douglass M. Bradham, Jr. (musc.edu)
  • Together, they published a monograph on the vital staining of connective tissue cells. (wikipedia.org)
  • Record, 23:18-19 with Evans, Herbert Mclean On the differential reactions to vital dyes exhibited by the two great groups of connective-tissue cells. (wikipedia.org)
  • On the differential reaction to vital dyes exhibited by the two groups of connective-tissue cells. (wikipedia.org)
  • Insight into the molecular regulation of ovarian ECM remodelling is potentially translatable to tissue remodelling elsewhere in the body. (bioscientifica.com)
  • Chen CM, Wang LF, Chou HC, Lang YD, Lai YP (2007) Up-regulation of connective tissue growth factor in hyperoxia-induced lung fibrosis. (springer.com)
  • Connective-Tissue Growth Factor Modulates WNT Signalling and Interacts With the WNT Receptor Complex," Development (2004) 131(9):2137-2147. (freepatentsonline.com)
  • Pre- and postnatal growth failure can be caused by mutations in the IGF1 (insulin-like growth factor 1) or IGF1R (insulin-like growth factor 1 receptor) genes. (ctgt.net)
  • Identification of novel factors that enhance β-cell proliferation and mass regeneration in vivo while retaining optimal function would serve as an ideal strategy for remediation of all forms of diabetes. (diabetesjournals.org)
  • Growth plate malformations included reduced proliferation zone and increased hypertrophic zone lengths . (temple.edu)
  • By in situ hybridization and immunohistochemistry, the respective mRNA moieties and proteins were localized in the tissue samples. (nih.gov)
  • Connective Tissue Growth Factor is a part of the CCN family of proteins. (prospecbio.com)
  • The insulin-like growth factor (IGF) binding proteins (IGFBPs) modulate the actions of the insulin-like growth factors in endocrine, paracrine, and autocrine settings. (pnas.org)
  • The insulin-like growth factor (IGF) binding proteins (IGFBPs) are a family of homologous proteins that regulate the biological activities of the IGFs and may also be capable of IGF-independent actions. (pnas.org)
  • the predicted proteins are products of "immediate-early genes" expressed after induction by serum, growth factors, or certain oncogenes ( 10 - 13 ). (pnas.org)
  • Connective Tissue Growth Factor belongs to the CCN family of proteins. (biovendor.com)
  • Functionally, the growth factor has been shown to regulate many aspects of cell behavior, including cell adhesion, migration, growth, chemotaxis, and the synthesis and accumulation of matrix proteins ( 3 ). (asnjournals.org)
  • 4 5 6 7 8 9 As well as stimulating the formation of scar tissue, these factors mediate subsequent wound contracture. (arvojournals.org)
  • Brigstock DR (1999) The connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (CCN) family. (springer.com)
  • These differential effects were due, in part, to elevated angiogenesis and were transforming growth factor (TGF)-β1 mediated. (aacrjournals.org)
  • These genes, along with those encoding the high-affinity IGFBPs 1-6, together constitute an IGFBP superfamily whose products function in IGF-dependent or IGF-independent modes to regulate normal and neoplastic cell growth. (pnas.org)
  • Connective Tissue Growth Factor Market 2018 is a comprehensive, professional report provides a detailed overview of major drivers, restraints, challenges, opportunities, current market trends and strategies impacting the global market along with estimates and forecast of revenue and share analysis. (positivenewspaper.com)
  • These studies have shown that reactive stroma initiates during prostatic intraepithelial neoplasia, exhibits a myofibroblast wound repair stromal phenotype, is tumor promoting, and is mediated, in part, by transforming growth factor (TGF)-β1 action ( 1 - 3 ). (aacrjournals.org)
  • In recent years, they are widely used for engineering of tissues such as skin and blood vessels due to their role in wound healing and secretion of extra cellular matrix (ECM) components (4-5). (ijbiotech.com)
  • Hale, TM & Lindsey, ML 2019, ' Connecting the Dots for Connective Tissue Growth Factor Roles in Cardiac Wound Healing After Myocardial Infarction ', JACC: Basic to Translational Science , vol. 4, no. 1, pp. 95-97. (elsevier.com)
  • Here we demonstrate that the absence of connective tissue growth factor in mice results in impaired hematopoiesis. (edu.au)
  • Brun JL, Feyler A, Chene G, Saurel J, Brun G and Hocke C: Long-term results and prognostic factors in patients with epithelial ovarian cancer. (spandidos-publications.com)
  • CP tissue samples were obtained from 40 patients (8 women, 32 men) undergoing pancreatic resection. (nih.gov)
  • Staining for PDGF receptors was also intense in the pannus tissue close to infiltrated bone and cartilage. (elsevier.com)
  • Recent years have seen a considerable emphasis on growth factors and the elucidation of their mode of function, which has led to the recognition that growth factors, their receptors as well as downstream elements of signalling associated with their function might be potential targets in therapeutic management of human diseases. (elsevier.com)
  • Humanised monoclonal antibodies raised against growth factor receptors have proved to be valuable for targeted cancer treatment and in patient management. (elsevier.com)
  • Also, a six-year ( 2012 to 2017 ) historic analysis is provided for Connective Tissue Growth Factor markets. (positivenewspaper.com)
  • In addition, TbetaR-I(ALK5) mRNA was increased in 50% of CP tissue samples (1.8-fold). (nih.gov)
  • Connective tissue growth factor promotes fibrosis downstream of TGF beta and IL-6 in chronic cardiac allograft rejection. (diff.org)
  • However, a sustained imbalance in these factors may lead to progressive renal sclerosis with eventual organ failure. (asnjournals.org)
  • However, Stro-1 and CD146 have been introduced as specific markers for MSCs but these markers seem limited to MSCs derived from bone marrow or renal tissue, while MSCs derived from adipose, for example, didn't express these markers (16). (ijbiotech.com)
  • We investigate cellular responses of CTP on a biomaterial surface covalently modified with epidermal growth factor (EGF) and find that surface-tethered EGF (tEGF) promotes both cell spreading and survival more strongly than saturating concentrations of soluble EGF. (mit.edu)
  • As early mesangial matrix expansion and the later chang- growth factors commonly control essential biological es of fibrosis in advanced diabetic nephropathy [10]. (deepdyve.com)
  • Globally, the diabetic nephropathy market is witnessing significant growth due to increasing prevalence of diabetes and obesity in different regions of the world. (cnbc.com)
  • However, stringent regulatory requirements and longer approval time for drugs as well as the lack of comprehensive therapeutic management for diabetic nephropathy are inhibiting the growth of diabetic nephropathy market. (cnbc.com)
  • In Europe, rise in healthcare expenditure for diabetes treatment and increasing prevalence of diabetes in the various part of Europe is boosting the growth of the European diabetic nephropathy market. (cnbc.com)
  • connective tissue growth factor ELISA Kits from BioVendor Laboratory Medicine, Inc. (biocompare.com)
  • Your search returned 1 connective tissue growth factor ELISA ELISA Kit across 1 supplier. (biocompare.com)
  • Human connective tissue growth factor expressed in Escherichia coli is a non-mitogenic inhibitor of apoptosis. (uzh.ch)
  • The cytokine Tumor Necrosis Factor Superfamily member 14, TNFSF14 (or LIGHT), is a controversial player in numerous diseases. (edu.au)
  • In addition, increasing R&D investments in drug discovery and development and raising awareness about diabetes and kidney-related disorders are also driving the growth of the market. (cnbc.com)
  • miRNAs have emerged as a new class of posttranscriptional regulators of gene function, and growing evidence indicates that miRNAs play key roles in growth, development, and stress responses of the heart. (ahajournals.org)
  • 10) Connective tissue growth factor plays an important role in tumor development and cancer progression, and it is found to be expressed in different types of cancer. (thefreelibrary.com)
  • To find a better model for studying the role of LFs in hyperoxia-induced lung fibrosis at the cellular level, we isolated LFs from the lung tissue of hyperoxia- and normoxia-exposed rat lungs on postnatal days 7, 14 and 21 for primary culture to study their proliferative behavior. (springer.com)
  • This study provides a better understanding of the functions of connective tissue growth factor within the bone marrow, showing the dual regulatory role of the growth factor in skeletogenesis and in stage-specific B lymphopoiesis. (edu.au)
  • It has been associated with fibrosis in various tissues including the kidney. (ovid.com)