Concanavalin A: A MANNOSE/GLUCOSE binding lectin isolated from the jack bean (Canavalia ensiformis). It is a potent mitogen used to stimulate cell proliferation in lymphocytes, primarily T-lymphocyte, cultures.Receptors, Concanavalin A: Glycoprotein moieties on the surfaces of cell membranes that bind concanavalin A selectively; the number and location of the sites depends on the type and condition of the cell.Methylmannosides: Mannosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with methyl alcohol. They include both alpha- and beta-methylmannosides.Lectins: Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition.Mannosides: Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of mannose with an alcohol to form an acetal. They include both alpha- and beta-mannosides.Agglutination: The clumping together of suspended material resulting from the action of AGGLUTININS.Plant Lectins: Protein or glycoprotein substances of plant origin that bind to sugar moieties in cell walls or membranes. Some carbohydrate-metabolizing proteins (ENZYMES) from PLANTS also bind to carbohydrates, however they are not considered lectins. Many plant lectins change the physiology of the membrane of BLOOD CELLS to cause agglutination, mitosis, or other biochemical changes. They may play a role in plant defense mechanisms.Lymphocyte Activation: Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.Lymphocytes: White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.Mitogens: Substances that stimulate mitosis and lymphocyte transformation. They include not only substances associated with LECTINS, but also substances from streptococci (associated with streptolysin S) and from strains of alpha-toxin-producing staphylococci. (Stedman, 25th ed)Spleen: An encapsulated lymphatic organ through which venous blood filters.T-Lymphocytes: Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.Wheat Germ Agglutinins: Lectins purified from the germinating seeds of common wheat (Triticum vulgare); these bind to certain carbohydrate moieties on cell surface glycoproteins and are used to identify certain cell populations and inhibit or promote some immunological or physiological activities. There are at least two isoforms of this lectin.Chromatography, Affinity: A chromatographic technique that utilizes the ability of biological molecules to bind to certain ligands specifically and reversibly. It is used in protein biochemistry. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Phytohemagglutinins: Mucoproteins isolated from the kidney bean (Phaseolus vulgaris); some of them are mitogenic to lymphocytes, others agglutinate all or certain types of erythrocytes or lymphocytes. They are used mainly in the study of immune mechanisms and in cell culture.Carbohydrates: The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.Immunologic Capping: An energy dependent process following the crosslinking of B CELL ANTIGEN RECEPTORS by multivalent ligands (bivalent anti-antibodies, LECTINS or ANTIGENS), on the B-cell surface. The crosslinked ligand-antigen receptor complexes collect in patches which flow to and aggregate at one pole of the cell to form a large mass - the cap. The caps may then be endocytosed or shed into the environment.Mannose: A hexose or fermentable monosaccharide and isomer of glucose from manna, the ash Fraxinus ornus and related plants. (From Grant & Hackh's Chemical Dictionary, 5th ed & Random House Unabridged Dictionary, 2d ed)Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Molecular Weight: The sum of the weight of all the atoms in a molecule.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Cell Membrane: The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.Hepatitis, Animal: INFLAMMATION of the LIVER in non-human animals.Pokeweed Mitogens: Proteins isolated from the roots of the pokeweed, Phytolacca americana, that agglutinate some erythrocytes, stimulate mitosis and antibody synthesis in lymphocytes, and induce activation of plasma cells.Glycopeptides: Proteins which contain carbohydrate groups attached covalently to the polypeptide chain. The protein moiety is the predominant group with the carbohydrate making up only a small percentage of the total weight.Receptors, Mitogen: Glycoprotein molecules on the surface of B- and T-lymphocytes, that react with molecules of antilymphocyte sera, lectins, and other agents which induce blast transformation of lymphocytes.Receptors, Drug: Proteins that bind specific drugs with high affinity and trigger intracellular changes influencing the behavior of cells. Drug receptors are generally thought to be receptors for some endogenous substance not otherwise specified.Colchicine: A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE).Interleukin-2: A soluble substance elaborated by antigen- or mitogen-stimulated T-LYMPHOCYTES which induces DNA synthesis in naive lymphocytes.Kinetics: The rate dynamics in chemical or physical systems.SepharoseElectrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Mice, Inbred BALB COligosaccharides: Carbohydrates consisting of between two (DISACCHARIDES) and ten MONOSACCHARIDES connected by either an alpha- or beta-glycosidic link. They are found throughout nature in both the free and bound form.Periodic Acid: A strong oxidizing agent.Canavalia: A plant genus of the family FABACEAE. Canavalia ensiformis is the source of CONCANAVALIN A.Macrophage-Activating Factors: Factors secreted by stimulated lymphocytes that prime macrophages to become nonspecifically cytotoxic to tumors. They also modulate the expression of macrophage cell surface Ia antigens. One MAF is INTERFERON-GAMMA. Other factors antigenically distinct from IFN-gamma have also been identified.Chromatography, Gel: Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.Hemagglutination: The aggregation of ERYTHROCYTES by AGGLUTININS, including antibodies, lectins, and viral proteins (HEMAGGLUTINATION, VIRAL).MethylglucosidesCell Line: Established cell cultures that have the potential to propagate indefinitely.MethylglycosidesPeanut Agglutinin: Lectin purified from peanuts (ARACHIS HYPOGAEA). It binds to poorly differentiated cells and terminally differentiated cells and is used in cell separation techniques.Nucleotidases: A class of enzymes that catalyze the conversion of a nucleotide and water to a nucleoside and orthophosphate. EC 3.1.3.-.Mice, Inbred Strains: Genetically identical individuals developed from brother and sister matings which have been carried out for twenty or more generations, or by parent x offspring matings carried out with certain restrictions. All animals within an inbred strain trace back to a common ancestor in the twentieth generation.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Drug-Induced Liver Injury: A spectrum of clinical liver diseases ranging from mild biochemical abnormalities to ACUTE LIVER FAILURE, caused by drugs, drug metabolites, and chemicals from the environment.Cytochalasin B: A cytotoxic member of the CYTOCHALASINS.GlucosamineThymus Gland: A single, unpaired primary lymphoid organ situated in the MEDIASTINUM, extending superiorly into the neck to the lower edge of the THYROID GLAND and inferiorly to the fourth costal cartilage. It is necessary for normal development of immunologic function early in life. By puberty, it begins to involute and much of the tissue is replaced by fat.Monosaccharides: Simple sugars, carbohydrates which cannot be decomposed by hydrolysis. They are colorless crystalline substances with a sweet taste and have the same general formula CnH2nOn. (From Dorland, 28th ed)Lymphokines: Soluble protein factors generated by activated lymphocytes that affect other cells, primarily those involved in cellular immunity.ThymidineImmunity, Cellular: Manifestations of the immune response which are mediated by antigen-sensitized T-lymphocytes via lymphokines or direct cytotoxicity. This takes place in the absence of circulating antibody or where antibody plays a subordinate role.Carbohydrate Sequence: The sequence of carbohydrates within POLYSACCHARIDES; GLYCOPROTEINS; and GLYCOLIPIDS.Cell Division: The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.Carbohydrate Conformation: The characteristic 3-dimensional shape of a carbohydrate.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.3,3'-DiaminobenzidineMannosidases: Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.T-Lymphocytes, Regulatory: CD4-positive T cells that inhibit immunopathology or autoimmune disease in vivo. They inhibit the immune response by influencing the activity of other cell types. Regulatory T-cells include naturally occurring CD4+CD25+ cells, IL-10 secreting Tr1 cells, and Th3 cells.

Fluorimetric multiparameter cell assay at the single cell level fabricated by optical tweezers. (1/3415)

A fluorimetric multi-parameter cell sensor at the single cell level is presented which makes it possible to observe the physiological behavior of different cell lines, different physiological parameters, and statistical data at the same time. Different cell types were immobilized at predefined positions with high accuracy using optical tweezers and adhesion promoting surface layers. The process is applicable to both adherent and non-adherent cells. Coating of the immobilization area with mussel adhesive protein was shown to be essential for the process. Intracellular proton and calcium concentrations in different cell classes were simultaneously imaged and the specific activation of T lymphocytes was demonstrated. This method should be especially useful for drug screening due to the small sample volume and high information density.  (+info)

Hydrophobic interaction of human, mouse, and rabbit interferons with immobilized hydrocarbons. (2/3415)

Interferons of human, mouse, and rabbit origin bind to straight chain hydrocarbons immobilized on agarose. The hydrophobic nature of binding is established by the following observations: (a) a positive correlation between the length of hydrocarbon ligand and the strength of interaction; (b) a stronger interaction with hydrocarbon ligands terminated with apolar rather than polar head groups; (c) a lack of dependence of binding on ionic strength and pH of the solvent; (d) a reversal of binding by ethylene glycol, a hydrophobic solute; (e) an increasing eluting efficacy of tetraalkylammonium ions with the length of their alkyl substituents. The hydrophobic interactions of human interferon underlie the efficiency of two-step chromatographic procedures. For example, human embryo kidney interferon can be purified about 3,600-fold by sequential chromatography on (a) concanavalin A-agarose, (b) octyl-agarose. Another two-step procedure: (a) concanavalin A-agarose, (b) L-tryptophan-agarose, gives about 10,000-fold purification. The overall recovery of interferon in both cases in close to 90%.  (+info)

Lectin receptor sites on rat liver cell nuclear membranes. (3/3415)

The presence and localization of lectin receptor sites on rat liver cell nuclear and other endomembranes was studied by light and electron microscopy using fluorescein and ferritin-coupled lectin conjugates. Isolated nuclei labelled with fluorescein-conjugated Concanavalin A (Con A) or wheat germ agglutinin (WGA) often showed membrane staining, which sometimes was especially bright on small stretches of the nuclear surface. Unlabelled nuclei and nuclei with a complete ring fluorescence were also seen. The nuclear fluorescence corresponded in intensity to that seen on the surface of isolated rat liver cells. Con A-ferritin particles were seldom detected on the cytoplasmic surface of the intact nuclear envelope. However, at places where the 2 leaflets of the envelope were widely separated or where the outer nuclear membrane was partly torn away, heavy labelling was seen on the cisternal surface of both the inner and outer nuclear membranes. Labelling with Con A-ferritin was also found on the cisternal side of rough endoplasmic reticulum present in the specimens. No labelling was seen on the cytoplasmic surface of mitochondrial outer membrane. The results demonstrate the presence of binding sites for Con A and WGA in nuclei and an asymmetric localization of these sites on the cisternal side of ribosome-carrying endomembranes in rat liver cells.  (+info)

Analysis of the stimulation-inhibition paradox exhibited by lymphocytes exposed to concanavalin A. (4/3415)

High doses of Concanavalin A (Con A), which normally inhibit T-lymphocyte stimulation as measured by increases in DNA synthesis, cause these lymphocytes to become committed to mitogenesis while also generating a dominant but reversible negative growth signal. The observed response to the stimulatory signal as measured by the rate of commitment to enter the S phase (i.e., the rate at which the stimulation becomes lectin independent) increases with lectin concentration even in the inhibitory range. The generation of this positive signal is prevented by treating the cells with colchicine. Cells that have become committed but are also simultaneously blocked from entering the S phase by the high doses of Con A can begin synthesizing DNA if the lectin is released by adding a competitive inhibitor of binding. Experiments done in agarose cultures in which lymphocytes are kept from contact with each other suggest that the reversible inhibitory signal is mediated by structures in the individual cells rather than as a result of agglutination. Continuously dividing cells of the lymphoid line P388 are also individually and reversibly inhibited by Con A. These findings are considered in terms of the relation of the inhibitory signal to the microtubular components of cell surface modulating assemblies made up of submembranous arrays of microtubules, microfilaments, and associated proteins.  (+info)

5'-Nucleotidase activity of mouse peritoneal macrophages. II. Cellular distribution and effects of endocytosis. (5/3415)

The diazonium salt of sulfanilic acid (DASA) can inactivate about 80% of the total 5'-nucleotidase of viable macrophages. The remaining 20% can be inactivated if the cells are first lysed in detergent, and presumably represents an intracellular pool of 5'-nucleotidase. The bulk of this pool may represent cytoplasmic vesicles derived from plasma membrane by endocytosis. This internal compartment is expanded up to threefold immediately after the cells have ingested a large latex load. This is consistent with previous observations on the internalization of 5'-nucleotidase in latex phagosomes. In latex-filled cells this intracellular pool of enzyme is inactivated over a few hours, and the cells then slowly increase their enzyme activity to nearly normal levels. However, 24 h after latex ingestion the metabolism of 5'-nucleotidase in these recovered cells is abnormal, as the rate of enzyme degradation is about twice the normal rate, and the DASA-insensitive enzyme pool in these cells is strikingly diminished. This may reflect effects of the accumulated indigestible particles on the fate of incoming pinocytic vesicles or on newly synthesized plasma membrane precursor. Another endocytic stimulus, concanavalin A, also reduces the total cell 5'-nucleotidase activity. This effect, which is time and temperature dependent, can be prevented by the competitive sugar alpha-methyl mannose. The concanavalin A inhibition can be reversed in the absence of new protein synthesis or in cells cultivated in serum-free conditions. It is not known whether the effect of concanavalin A on 5'-nucleotidase depends upon the interiorizaiton of plasma membrane or is strictly associated with events at the cell surface.  (+info)

Effect of sodium butyrate on lymphocyte activation. (6/3415)

Butyrate, in relatively low concentrations, has been shown to induce synthesis of enzymes, cause changes in cell morphology, and inhibit growth of a variety of mammalian cells in tissue culture (reviewed in [1]). In this communication, we report our observations on the effect of butyrate on lymphocyte activation. Butyrate completely and reversibly inhibits mitogen-induced blast formation. We present evidence that it does not interfere with the binding of mitogens, that it does not inhibit a number of the "early" reactions involved in activation, and that it does not affect ongoing DNA synthesis for an extended period of time. However, butyrate rapidly inhibits any increase in the rate of DNA synthesis.  (+info)

Concanavalin A-mediated binding and sphering of human red blood cells by homologous monocytes. (7/3415)

Human red blood cells sensitized with concanavalin A became bound to homologous peripheral blood monocytes. Binding occured at a concentration of 10(5) molecules of tetrameric Con A per red blood cell (RBC) and increased with additional Con A. RBC binding began within 5 min and was maximal at 90 min. Phagocytosis of sensitized RBCs was minimal. RBC attachment was prevented by 0.01 M alpha-methyl-D-mannopyranoside, and, once the RBC-monocyte rosette was established, bound RBCs were largely removed with this specific saccharide inhibitor of Con A. RBCs attached to monocytes became spherocytic and osmotically fragile. The recognition of concanavalin A (Con A)-coated RBCs was not mediated through the monocyte IgG-Fc receptor. These studies demonstrate that, like IgG and C3b, Con A is capable of mediating the binding of human RBCs to human monocytes. Red cells so bound are damaged at the monocyte surface.  (+info)

Binding partners for the myelin-associated glycoprotein of N2A neuroblastoma cells. (8/3415)

The myelin-associated glycoprotein (MAG) has been proposed to be important for the integrity of myelinated axons. For a better understanding of the interactions involved in the binding of MAG to neuronal axons, we performed this study to identify the binding partners for MAG on neuronal cells. Experiments with glycosylation inhibitors revealed that sialylated N-glycans of glycoproteins represent the major binding sites for MAG on the neuroblastoma cell line N2A. From extracts of [3H]glucosamine-labelled N2A cells several glycoproteins with molecular weights between 20 and 230 kDa were affinity-precipitated using immobilised MAG. The interactions of these proteins with MAG were sialic acid-dependent and specific for MAG.  (+info)

Moebius U, Manns M, Hess G, Kober G, Meyer zum Buschenfelde KH, Meuer SC. T cell receptor gene rearrangements of T lymphocytes infiltrating the liver in chronic active hepatitis B and primary biliary cirrhosis (PBC): oligoclonality of PBC-derived T cell clones. Eur J Immunol 1990;20:889-896 ...
Sigma-Aldrich offers abstracts and full-text articles by [Sainan Li, Yujing Xia, Kan Chen, Jingjing Li, Tong Liu, Fan Wang, Jie Lu, Yingqun Zhou, Chuanyong Guo].
TY - JOUR. T1 - Cell-cell contact with proinflammatory macrophages enhances the immunotherapeutic effect of mesenchymal stem cells in two abortion models. AU - Li, Yanhong. AU - Zhang, Di. AU - Xu, Ling. AU - Dong, Lin. AU - Zheng, Ji. AU - Lin, Yikong. AU - Huang, Jiefang. AU - Zhang, Yanyun. AU - Tao, Yu. AU - Zang, Xingxing. AU - Li, Dajin. AU - Du, Meirong. PY - 2019/12/1. Y1 - 2019/12/1. N2 - Mesenchymal stem cells (MSCs), which are pluripotent cells with immunomodulatory properties, have been considered good candidates for the therapy of several immune disorders, such as inflammatory bowel diseases, concanavalin A-induced liver injury, and graft-versus-host disease. The embryo is a natural allograft to the maternal immune system. A successful pregnancy depends on the timely extinction of the inflammatory response induced by embryo implantation, followed by the switch to a tolerant immune microenvironment in both the uterus and the system. Excessive infiltration of immune cells and serious ...
TY - JOUR. T1 - Recognition of septanose carbohydrates by concanavalin A. AU - Castro, Steve. AU - Duff, Michael. AU - Snyder, Nicole L.. AU - Morton, Martha D. AU - Kumar, C. V.. AU - Peczuh, Mark W.. PY - 2005/11/7. Y1 - 2005/11/7. N2 - The ability of the jack bean lectin concanavalin A (ConA) to bind seven membered ring (septanose) monosaccharides has been investigated by isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR spectroscopy.. AB - The ability of the jack bean lectin concanavalin A (ConA) to bind seven membered ring (septanose) monosaccharides has been investigated by isothermal titration calorimetry (ITC) and saturation transfer difference (STD) NMR spectroscopy.. UR - http://www.scopus.com/inward/record.url?scp=27844440774&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=27844440774&partnerID=8YFLogxK. U2 - 10.1039/b509243d. DO - 10.1039/b509243d. M3 - Article. VL - 3. SP - 3869. EP - 3872. JO - Organic and Biomolecular ...
The intravenous injection of the plant lectin concanavalin A (ConA) is a widely used model for acute immune-mediated hepatitis in mice. In contrast to several other models for acute hepatic damage, ConA-induced injury is primarily driven by the activation and recruitment of T cells to the liver. Hen …
Strain AS rats respond with two populations of cytotoxic T lymphocytes to stimulation in vitro by the major histocompatibility complex (MHC)-incompatible strain HL rat tumor (HL-A2T2). One is specific for MHC alloantigens present on both HL-A2T2 and normal HL targets, the other is tumor specific. The activation of these killer cells requires helper T lymphocytes. The tumor-specific helper cells depend on syngeneic radioresistant accessory cells to present the tumor antigens in an immunogenic form. The appropriate helper-accessory cell interaction results in the production of soluble factors which then induce the maturation of precursor cells into effective killer cells. Studies with a procedure for inducing negative selection of T cells in vivo showed that short-term exposure to HL-A2T2 tumor induced selection only for TH but not cytotoxic T lymphocyte precursors (CTLp). Simultaneous injection of supernatants from concanavalin A-activated spleen cell cultures, however, did produce selection of ...
Chemical signaling between cells in multicellular organisms is frequently mediated by cell-surface receptors. The receptors for neurotransmitters, protein hormones, growth factors, and plant lectins are a few of the many known examples of these important membrane components. In this exercise, students examine the cell location and properties of the receptor for the lectin concanavalin A. In the first experiment of the series, students use a concanavalin A-peroxidase complex in a microscopic assay to show that the specific receptor is found on the surface of their own cheek epithelial cells. In the second part of the exercise, students study the characteristic hemagglutination reaction that is elicited by concanavalin A and learn that the reaction is due to multiple sites for receptor binding on the concanavalin A molecule. It is known that concanavalin A binds selectively to mannose residues on the cell-surface glycoprotein receptor. This specificity is confirmed in the final experiment of the ...
Mechanism of human lymphocyte stimulation by concanavalin A: role of valence and surface binding sites.: A monovalent form of concanavalin A (m-Con A) has been
Mediators of Inflammation is a peer-reviewed, Open Access journal that publishes original research and review articles on all types of inflammatory mediators, including cytokines, histamine, bradykinin, prostaglandins, leukotrienes, PAF, biological response modifiers and the family of cell adhesion-promoting molecules.
Schmidtke, J R.; Ray, P K.; and Simmons, R L., "Effects of cell-bound phytohemagglutinin and concanavalin a on the mixed leucocyte response of mouse spleen cells in vitro." (1974). Subject Strain Bibliography 1974. 1861 ...
A method for the visualization of concanavalin A (Con A) binding sites by electron microscopy of glycol methacrylate sections is presented. This method, which is an application of the alkaline...
A flow cytometry assay that can be used to directly determine the proportion of activated T lymphocytes in human whole blood samples after stimulation with concanavalin A is presented here
DIAZ MARIA R.M. , TAKAHASHI TADASHI C , TAKESHIMA KAZUHITO , TAKATA KENZO The neural-inducing activity of ventral mesoderm aged after Con A treatment was examined in detail. To determine the time at which this function was attained, Con A-treated ventral mesoderm was incuba … Zoological Science 8(4), p705-712, 1991-08 NDL Digital Collections ...
Gentaur molecular products has all kinds of products like :search , Biotium \ Concanavalin A, CF350 conjugate \ 29015 for more molecular products just contact us
The adherent culture of rat thoracic aorta cells featured in this section was stained with Alexa Fluor 488 conjugated to the lectin concanavalin A, which selectively binds to alpha-mannopyranosyl and alpha-glucopyranosyl residues (primarily in the endoplasmic reticulum).
This finding was the first discovery of the impact of chronic DU exposure on B-cell maturation, and the function of the mature B-cells in recognising antigens and mediating. specific immune responses was thereby affected. The impact of DU on humoral immunity was apparently similar to that of radiation. Exposure to low doses of gamma external irradiation (10 cGy, 1 cGy/min) activated the thymus-dependent humoral immune and enhanced polyclonal B-cells in mice (Sharetskiĭ et al., 2000). It should be clarified that both immunosuppression and immune stimulation are immunotoxic reactions (Gleichmann et al., 1989). Third, long-term exposure to DU led to changes in the cellular immune function in the DU300 group (300 mg/kg), including decreased proliferative ability of ConA-stimulated Inhibitor Library datasheet splenic T cells, suppression of delayed-type hypersensitivity, decrease in the number of CD3+ cells, and decrease in the ratio of CD4+/CD8+ splenic T cells.. In Epacadostat purchase the DU30 ...
MAMFKTLSFL FLLCFQIHGT VAADENSLFS FNGFVKSPSF DKRVALFGDS KLVNDSSLIQ LTDSVTRSVG RVVYKKPINL FKGKQRNSRS FSTHFSFSSM SNEIGDDVLA FVMVPSSLDL SLFGKKDNSS SALGFLSQYA KNETVVAVEF GISRTGNSAR ILVGRPESAE IRNLSFVGDL MMDNGRTLSC MIEYEASSKR MMVRFRKSGS VKLFDPFFSF SVDLAKLWKG GEVTVGLSSA NGNSSSKAHF LHSWRFEIRH PPPMWMHSVP LEPNEVSKEE ENGRGRSECT RKMLGALIFG AACGALGALL GLYLWTICRV RRSMAVVPEE CAIEQGKK ...
This study aimed to evaluate the effect of mesenchymal stem cell (MSC)-derived exosomes on an immune-induced liver injury model. MSCs show a unique function to modulate immune reaction although the molecular mechanisms are still under investigation. Exosomes are a nanoparticle containing microRNA and many ligands and are recognized as important factors secreted from MSC to express their function. This research is undertaken to evaluate the effect of MSC-derived exosome on concanavalin-A (con-A)-induced liver injury. Exosomes were collected from the supernatant of MSC from the bone marrow of C57B6 mice with ultracentrifugation. The collected exosomes or MSCs were injected intravenously into liver injury mice that had been prepared by the intravenous con-A injection. Liver and serum samples were collected 24 h later to evaluate the macro- and microscopic images, the alanine aminotransferase (ALT), and cytokine messenger RNA (mRNA) expression levels. Phenotypical change of non-parenchymal liver cells was
Peroxisome proliferator activated receptor alpha (PPARα), a regulator of enzymes involved in β oxidation, has been reported to influence lymphocyte activation. The purpose of this study was to determine whether PPARα plays a role in T cell-mediated hepatitis induced by Concanavalin A (ConA). Wild type (wt) or PPARα-deficient (PPARα−/−) mice were treated with ConA (15 mg/kg) by intravenous injection 0, 10 or 24 h prior to sacrifice and serum and tissue collection for analysis of tissue injury, cytokine response, T cell activation and characterization. Ten and 24 h following ConA administration, wt mice had significant liver injury as demonstrated by serum transaminase levels, inflammatory cell infiltrate, hepatocyte apoptosis, and expression of several cytokines including interleukin 4 (IL4) and interferon gamma (IFNγ). In contrast, PPARα−/− mice were protected from ConA-induced liver injury with significant reductions in serum enzyme release, greatly reduced inflammatory cell infiltrate,
The fatty acid compositions of the neutral lipid and phospholipid fractions of rat lymph node lymphocytes were characterized. Stimulation of rat lymphocytes with the T-cell mitogen concanavalin A resulted in significant changes in the fatty acid composition of both neutral lipids and phospholipids (a decrease in the proportions of stearic, linoleic and arachidonic acids and an increase in the proportion of oleic acid). Membrane fluidity was measured using nitroxide spin-label e.s.r., and increased during culture with concanavalin A. Culturing the lymphocytes in the absence of mitogen did not affect fatty acid composition or membrane fluidity. The uptake and fate of palmitic, oleic, linoleic and arachidonic acids were studied in detail; there was a time-dependent incorporation of each fatty acid into all lipid classes but each fatty acid had a characteristic fate. Palmitic and arachidonic acids were incorporated principally into phospholipids whereas oleic and linoleic acids were incorporated in similar
Hill, D J. and Borysenko, J Z., "Restriction of patching of bound concanavalin a after incorporation of arachidonic acid into the plasma membrane of virally transformed fibroblasts." (1979). Subject Strain Bibliography 1979. 3532 ...
TY - JOUR. T1 - The role of lymphokines in immunogenic uveitis. AU - Liu, S. H.. AU - Prendergast, R. A.. AU - Silverstein, A. M.. PY - 1983. Y1 - 1983. N2 - Lymphokines were prepared from rabbit lymph node cells specifically activated in vitro with the insoluble antigen ovalbumin or nonspecifically activated with the insoluble mitogen concanavalin A. Intravitreal injection of either lymphokine caused uveitis in the normal rabbit eye, more severe and more prolonged with the concanavalin A-activated supernatants than with the ovalbumin-activated supernatants. The most striking difference between the two lymphokine preparations was in their ability to induce a secondary intraocular antibody response in the trinitrophenyl bovine gamma globulin-primed recipient. Concanavalin A supernatants stimulated polyclonal B cell activation with appreciable anti-TNP responses in animals primed either systemically or locally. In contrast, ovalbumin supernatants stimulated an intraocular anti-TNP response only in ...
The liver has been well recognized not only as an important metabolic organ, but also as an important site for immune responses. It is widely accepted that immune-mediated liver injury plays a central role in the pathophysiology of many liver diseases (36, 37). The remarkable enrichment of NKT cells in mouse liver has attracted much research attention to explore the role of this unique cell population in the pathogenesis of hepatic diseases, and increasing evidence has suggested that NKT cells are indeed involved in the development of many liver diseases (14). Activation of NKT cells with α-GalCer causes moderate liver injury, which has been recognized as a useful model to investigate immune-mediated liver injury (14, 17-19). The major and novel findings of this study are: 1) α-GalCer injection causes a remarkable increase in IRF-1 expression in mouse liver; 2) α-GalCer-induced IRF-1 upregulation in vivo is TNF-α- and IFN-γ-dependent; 3) IRF-1 plays an important role in mediating ...
Abstract: Glucagon and concanavalin A were administered into cell cultures of mice peritoneal macrophages and human intima aorta simultaneously with atherogenous blood serum, obtained from patients with ischemic heart disease, or with acetylated and native low density lipo-proteins. Their effect was dissimilar: glucagon decreased accumulation of intracellular cholesterol and the rate of 3H-thymidine incorporation into these cells, while concanavalin A increased the patterns studied. Cellular lysosomes appear to participate in atherogenesis, these results suggest that regulation of lysosomal apparatus may occur at the step of secondary lysosomes formation ...
We describe an approach to accelerate the search for competitive inhibitors for carbohydrate-recognition domains (CRDs). Genetically encoded fragment-based-discovery (GE-FBD) uses selection of phagedisplayed glycopeptides to dock a glycan fragment at the CRD and guide selection of Synergistic peptide motifs adjacent to the CRD. Starting from concanavalin A (ConA), a mannose (Man)-binding protein, as a bait, we narrowed a library of 10(8) glycopeptides to 86 leads that share a consensus motif, Man-WYD. Validation of synthetic leads yielded Man-WYDLF that exhibited 40 50-fold enhancement in affinity over methyl alpha-D-mannopyranoside (MeMan). Lectin array Suggested specificity: Man-WYD derivative bound only to 3 out of 17 proteins-ConA, LcH, and PSA-that bind to Man. An X-ray structure of ConA.:Man-WYD proved that the trimannoside core and Man-WYD exhibit identical CRD docking; but their extra-CRD binding modes are significantly. different. Still, they have comparable affinity and se... ...
Sigel et al.(J. Am. Chern. Soc., 118(16), 3789-3800,1996) reported the efficacy of polymers containing sialoside groups in inhibiting the adhesion of influenza virus to, erythrocytes. They delineated the contributions of enhanced substrate ligand binding and steric considerations to efficiency of inhibition. These investigators reported sialic acid ligands, which can be exploited for the inhibition of the influenza virus. Monomeric inhibitor requires a higher concentration for inhibition since they are required to occupy at least half of the sialic acid binding sites on the virus, whereas the high molecular weight inhibitors need only a few attachments to achieve the same. Dimick et al. (J. Am. Chern. Soc., 121: 44,10286,1999) reported the molecular cluster glycoside effects, and the synthesis of polyvalent ligands for the plant lectin concanavalin A ...
Citation: Tanna, S., Sahota, T., Taylor, M. et al. (2002). Covalent coupling of concanavalin A to a Carbopol 934P and 941P carrier in glucose-sensitive gels for delivery of insulin. Journal Pharmacy and Pharmacology, 54 (11) pp.1461-1469. ...
Beneficial effects of Va and/or Zn supplementation of children in NE Thailand are described in a companion abstract. In the same study, blastogenic response (BR) of T-lymphocytes to concanavalin-A (ConA) and PPD were assayed in cultures containing mononuclear cells (MNC) or whole blood (WB). Methods were previously described. Children were previously vaccinated with BCG. BR to ConA of MNC or WB from children supplemented with VA, Zn, VA + Zn or placebo were similar. BR to PPD of MNC was higher in children receiving VA + Zn than placebo, but not in children supplemented with VA or Zn alone. Data indicate that children with suboptimal VA and Zn nutriture supplemented with , 2 times RDA of these nutrients showed enhanced cellular immunity to PPD. This observation is relevant to BCG immunization program and thus may benefit public health. ...
Mitogenic signals from concanavalin A and phytohemagglutinin were shown to undergo summation in time under conditions when each mitogen separately, if the duration of contact with lymphocytes during...
Department of Chemistry, UBC Faculty of Science. Vancouver Campus. 2036 Main Mall. Vancouver, BC Canada V6T 1Z1. Tel: 604.822.3266. Fax: 604.822.2847. ...
CONFERENCE OF NAVAL ASSOCIATIONS CONA Website The CONA Newsletter No 1 July 2014 Revealed at last in all its magnificence the Naval Service Memorial is Dedicated at the National Memorial
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1. The luminol-dependent chemiluminescence of rat thymocytes responding to concanavalin A can be resolved into glucose-dependent and glucose-independent portions. 2. The glucose-dependent portion, supported by D-glucose and D-mannose oxidation, is inhibited by catalase (200 microgram/ml), amobarbital (1 mM) and hexose analogues that block D-glucose uptake. Thus concanavalin A may activate, transiently, an NAD(P)H oxidase that utilizes reducing equivalents derived from the oxidation of exogenous glucose to give dismutation products of O2- (including H2O2) as its major products. 3. The glucose-independent portion is inhibited by eicosa-5,8,11,14-tetraynoic acid but not by indomethacin. It may therefore be associated with the conversion of hydroperoxy intermediates of arachidonic acid metabolism to hydroxy products by the lipoxygenase pathway. 4. Preincubation of thymocytes for 18 h in serum-free medium enhances the subsequent chemiluminescent response to concanavalin A severalfold and evokes the ...
In this paper, we demonstrated that chronic ethanol consumption markedly potentiates T cell-mediated hepatitis induced by injection of Con A in mice. Furthermore, it was determined that ethanol potentiation of T cell-mediated hepatitis is due mainly to enhancing the NF-κB signaling pathway and the subsequent induction of NF-κB-controlled chemokines and adhesion molecule expression in the liver. As shown in Fig. 5, B and C, NF-κB binding, IκB phosphorylation, and IκB degradation after Con A injection were significantly enhanced in ethanol-fed mice compared with pair-fed mice. Consistent with this finding, ICAM-1, MIP-1α, MIP-1β, MIP-2, and MCP-1 expression, which are controlled by NF-κB (11, 18, 37), are significantly prolonged in ethanol-fed mice after Con A injection compared with pair-fed mice. It has been reported (31, 32) that ethanol consumption causes activation of NF-κB and subsequent induction of chemokines and adhesion molecules in rodent livers as well as in monocytes from ...
TY - JOUR. T1 - Killing of Trypanosoma brucei by Concanavalin A. T2 - Structural basis of resistance in glycosylation mutants. AU - Acosta-Serrano, Alvaro. AU - Cole, Robert N. AU - Englund, Paul T.. PY - 2000/12/8. Y1 - 2000/12/8. N2 - Concanavalin A (Con A) kills procyclic (insect) forms of Trypanosoma brucei by binding to N-glycans on EP-procyclin, a major surface glycosyl phosphatidylinositol (GPI)-anchored protein which is rich in Glu-Pro repeats. We have previously isolated and studied two procyclic mutants (ConA 1-1 and ConA 4-1) that are more resistant than wild-type (WT) to Con A killing. Although both mutants express the same altered oligosaccharides compared to WT cells, ConA 4-1 is considerably more resistant to lectin killing than is ConA 1-1. Thus, we looked for other alterations to account for the differences in sensitivity. Using mass spectrometry, together with chemical and enzymatic treatments, we found that both mutants express types of EP-procyclin that are either poorly ...
TY - JOUR. T1 - Formation of homogeneous cross-linked lattices between oligomannose type glycopeptides and concanavalin A. AU - Islam Khan, M.. AU - Bhattacharyya, Lokesh. AU - Brewer, Curtis F.. PY - 1988/5/16. Y1 - 1988/5/16. N2 - Certain oligomannose type glycopeptides have previously been shown to be bivalent for binding to concanavalin A, and to give quantitative precipitation profiles with the protein that consist of single peaks which correspond to the binding stoichiometry of glycopeptide to protein monomer (1:2) (Bhattacharyya, L., Ceccarini, C., Lorenzoni, P., and Brewer, C.F. (1987) J. Biol. Chem. 262, 1288-1293). In the present study, equimolar mixtures of two oligomannose type glycopeptides, a Man-6 and a Man-9 glycopeptide, gives a quantitative precipitation profile which shows two protein peaks. Each glycopeptide was radiolabelled with 3H or 14C, and the the precipitation profiles of the individual glycopeptides in the mixture determined. The results show that the radioactivity ...
BioAssay record AID 196412 submitted by ChEMBL: Percent inhibition of Concanavalin A stimulated cell proliferation of rat spleen lymphocytes at 10 uM; ND is not determined.
Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean, Canavalia ensiformis. It is a member of the legume lectin family. It binds specifically to certain structures found in various sugars, glycoproteins, and glycolipids, mainly internal and nonreducing terminal α-D-mannosyl and α-D-glucosyl groups. ConA is a plant mitogen, and is known for its ability to stimulate mouse T-cell subsets giving rise to four functionally distinct T cell populations, including precursors to suppressor T-cell; one subset of human suppressor T-cells as well is sensitive to ConA. ConA was the first lectin to be available on a commercial basis, and is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities on the surface of various cells. It is also used to purify glycosylated macromolecules in lectin affinity chromatography, as well as to study immune regulation by various immune cells. Like most lectins, ConA is a ...
Treatment of responder cells with monoclonal anti-Ly-1,2 antibodies plus complement in vitro completely eliminated their ability to generate azobenzenearsonate (ABA)-specific cytolytic T lymphocytes (CTL). However, addition of the concanavalin A-stimulated supernatants of rat spleen cells (Con A-Sup) can fully reconstitute the response. Therefore, Lyt-1,2-bearing T cells are required for the generation of ABA-specific CTL, and such requirement can be replaced by factors present in the Con A- sup. Suppressor T cells (Ts), when adoptively transferred into naive recipients, will inhibit the in vivo priming of CTL. This inhibition can also be reversed by in vitro addition of Con A-Sup. furthermore, mice serving as donors of Ts also show profound unresponsiveness when primed and restimulated in vitro. In contrast to the Ts-mediated inhibition, in vitro addition of Con A-Sup was unable to abolish the unresponsiveness observed in these cultures. Thus, we identified two unresponsive states in a ...
It is now clear that estrogen not only modulates the differentiation and function of reproductive systems, but it also profoundly regulates the immune system of normal and autoimmune individuals. An important mechanism by which estrogen regulates the immune system is by altering the secretion and/or response to cytokines. We hypothesized that estrogen may alter the levels and/or response to IFN-g, a prototype Th1 cytokine, that plays a pivotal role in immunity against intracellular infections and in many autoimmune and inflammatory disorders. We found that estrogen treatment tended to upregulate the secretion of IFN-g protein and mRNA expression from Concanavalin-A (Con-A)-activated splenic lymphocytes. Impressively, we found that splenocytes from estrogen-treated mice when activated with Con-A also resulted in increased release of nitric oxide compared to placebo-treated mice. Furthermore, Con-A-activated splenocytes from estrogen-treated mice also had upregulated iNOS mRNA, iNOS protein, and ...
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Page contains details about Concanavalin A/bovine serum albumin-incorporated LA-conjugated Tetronic® 1107 micelles aggregates . It has composition images, properties, Characterization methods, synthesis, applications and reference articles : nano.nature.com
Magnetic resonance studies of concanavalin A: location of the binding site of alpha-methyl-D-mannopyranoside.: The longitudinal nuclear magnetic relaxation time
The adherent culture of Indian Muntjac deer skin fibroblasts presented in this section was stained with Texas Red conjugated to the lectin concanavalin A.
1ONA: A structure of the complex between concanavalin A and methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside reveals two binding modes.
Description of disease T-suppressor count. Treatment T-suppressor count. Symptoms and causes T-suppressor count Prophylaxis T-suppressor count
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Macrophage suppression has been shown to be mediated by a unique, low molecular weight fraction of murine serum. The present investigation involves the in vitro production of this macrophage modulator (suppressor) by Concanavalin A-stimulated spleen cells. Spleen cell culture supernatant containing macrophage suppressor factor (MSF) caused a significant decrease in in vitro phagocytosis of Listeria monocytogenes by non-elicited peritoneal macrophages. The molecular weight of MSF was determined by ultrafiltration to be less than 10,000, and the modulating activity of MSF was not altered by heating at 100°C for 30 minutes or freezing at -70°C for six months. MSF is resistant to treatment with Pronase E, but is, however, sensitive to acid hydrolysis. Activity of MSF in spleen cell culture supernatants from normal mice does not differ from supernatants from mice immunized with L. monocytogenes. It was therefore concluded that MSF is not affected by antigenic stimulation and is apparently produced
Define agglutinability. agglutinability synonyms, agglutinability pronunciation, agglutinability translation, English dictionary definition of agglutinability. adj. Capable of being agglutinated. ag·glu′tin·a·bil′i·ty n
Vicia faba lectin contained 40-57% beta-conformation, 4-23% alpha-conformation along with random coil at pH 7.2 depending upon the analytical methods used. The percentage of beta-conformation increased with the addition of N-acetyl-D-glucosamine or methyl alpha-D-mannopyranoside. The structural transitions of V. faba lectin were affected by alkali at pH 9.6 and 10.6. Binding constants and free energy changes for the interaction between V. faba lectin and N-acetyl-D-glucosamine and methyl alpha-D-mannopyranoside were estimated at pH 7.2 using the c.d. and fluorescence methods. ...
TY - JOUR. T1 - Investigation of K+ channel expression in human peripheral lymphocytes of healthy donors by means of flow cytometry. AU - Krjukova, J.. AU - Osna, N.. AU - Pilmane, M.. PY - 2000/1/1. Y1 - 2000/1/1. N2 - Evaluation of different types of K+ channel expression was performed in resting and PHA (phytohemagglutinine)-activated human peripheral lymphocytes (HPL) of healthy donors by means of flow cytometry. In resting peripheral lymphocytes, the application of kaliotoxin (a selective blocker for voltage-dependent K+ (K(V)) channels), K(V) resulted in pronounced depolarization of lymphocyte membrane potential, with further promotion in the presence of thapsigargin (compound discharging Ca(i) from endoplasmic reticulum). In activated HPL, the expression of various types of K+ channels was estimated utilizing cell-cycle analysis data. In contrast to the resting cells, kaliotoxin-induced depolarization of membrane potential in PHA-activated lymphocytes of the G0/G1 phase was not enhanced ...
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Semantic Scholar extracted view of A modified, quantitative assay for substances that inhibit the migration of macrophages and other leucocytes: the use of concanavalin A as a reference reagent. by Evelyn Fenton et al.
Cancer, Glycoproteins, Proteins, Tumor, Cancer Stem Cells, Stem Cells, Patients, Tissue, Chromatography, Glycoprotein, Cell, Cells, Stem, Glioblastoma, Tumors, Pancreatic Cancer, Serum, Identification, Concanavalin A, Human
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The immunomodulatory effects of the ethanol extract of Gynostemma pentaphyllum (GP-EX) were examined in electric footshock (EFS)-stressed mice. The mice were orally administered various doses of GP-EX for 7 days before exposure to EFS (duration: 3 min, interval: 10 s, intensity: 2 mA) once a day from day 8 for 14 days with continuous daily feeding of GP-EX. Oral administration of GP-EX to mice prevented EFS stress-induced immunosuppression as determined by the lymphoid organ (thymus and spleen) weight and cellularity. In addition, oral administration of GP-EX restored EFS-suppressed functional properties of mature lymphocytes in terms of concanavalin A-induced proliferation of splenocytes and lipopolysaccharide-induced cytokine production (TNF-α, IL-1β). Furthermore, we found that mice that were orally administered with GP-EX generated much more potent ovalbumin-specific cytotoxic T lymphocyte responses upon intravenous ovalbumin injection compared to the untreated controls. These results demonstrate
Imidoesters amidinate free amino groups and produce inter- and intramolecular covalent bonds. To determine whether imidoesters influenced lymphocyte transformation, human peripheral blood or calf lymph node lymphocytes were cultured with dimethyladipimate (DMA), a bifunctional (cross-linking) imidoester, or methyl acetimidate (MAC), a monofunctional (noncross-linking) imidoester. Both DMA and MAC decreased the rate of endogenous DNA synthesis in a dose-dependent fashion. In further work, lymphocytes were treated with Phaseolus vulgaris phytohemagglutinin, concanavalin A, or periodate. DMA (1 mm) decreased DNA synthesis in P. vulgaris phytohemagglutinin-stimulated human cells by 65%, Concanavalin A-stimulated cells by 98.2%, and periodate-stimulated cells by 85%. Similar results were obtained with 1 mm MAC. Inhibition by DMA was slightly greater than was the inhibition by MAC. Decreased DNA synthesis resulted if DMA was added to P. vulgaris phytohemagglutinin-stimulated human lymphocytes at ...
Patients with minimal change nephrotic syndrome (MCNS) frequently have suppressed in vivo and in vitro immune responsiveness of uncertain etiology. Because increased suppressor cell activity has been associated with this disease, urines from MCNS patients were screened for activity of the lymphokine soluble immune response suppressor (SIRS), a product of concanavalin A- or interferon-activated suppressor T cells. Urines from untreated MCNS patients suppressed polyclonal plaque-forming cell responses of cultured splenocytes. This suppressive activity was identified as human SIRS by the following functional and physical criteria: molecular weight estimated by gel filtration; kinetics of suppression; inhibition of suppression by catalase, levamisole, and 2-mercaptoethanol; abrogation of activity by acid or protease treatment; elution pattern on high performance liquid chromatography; and cross-reactivity with monoclonal antimurine SIRS antibodies. Suppressive activity disappeared from urine after ...
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TY - JOUR. T1 - Generation of alloreactive anti-leukemic cytotoxic T lymphocytes with attenuated GVHD properties from haploidentical parents in childhood acute lymphoblastic leukemia. AU - Jurickova, I.. AU - Waller, E. K.. AU - Yeager, Andrew M. AU - Boyer, M. W.. PY - 2002/11. Y1 - 2002/11. N2 - We sought to generate alloreactive leukemia-reactive cytotoxic T lymphocytes from haploidentical parents by culture of peripheral blood mononuclear cells in vitro with irradiated leukemic blasts and IL-2 from children with ALL. After 21 days in culture the mean cytotoxicity of haploidentical lymphocytes against ALL blasts was 38% (n = 11). Cultured parental CTL were not leukemia specific but alloreactive as evidenced by equivalent cytotoxicity against stimulating ALL blasts and ConA-stimulated blasts from the other parent. The cultures yielded primarily CD8+ T cells (59%). Irradiation of CTL limited proliferation by 96% but had no short-term effects on leukemia reactive cytotoxicity, suggesting a means ...
Description of disease T-suppressor cell. Treatment T-suppressor cell. Symptoms and causes T-suppressor cell Prophylaxis T-suppressor cell
Chronic renal failure (CRF) causes immunosuppresion in humans and is thought to be one of the causes of noninfectious secondary immunosuppression in dogs. Hematological, biochemical, and immunological examinations were performed on blood samples obtained from dogs in various stages of CRF. The number of dogs with lymphopenia increased with the progression of clinical signs. All main subsets of lymphocytes were decreased, but more considerable reduction was detected in B-cells, Tc-cells, and NK cells. Depressed lymphocyte response to concanavalin A and pokeweed mitogen was found in dogs with severe clinical signs and lymphopenia. Our results, showing impaired immunological functions, are similar to results obtained from uremic humans, suggesting that infection may be an important complication in dogs with CRF ...
Bead- and column-based separation methods rely heavily on the speed and ease of affinity binding systems. Ligands such as streptavidin, antibodies and lectins are used both to capture specifically-tagged targets and for the isolation of cells and biomolecules that naturally express the ligand binding partner. The unique saccharide-binding properties of plant lectins, such as Concanavalin A (Con A) have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate. Lectins have additionally been used in cell adhesion studies, to effect lymphocyte activation, and to explore carbohydrate-based therapeutics.. 了解更多 ...
Bead- and column-based separation methods rely heavily on the speed and ease of affinity binding systems. Ligands such as streptavidin, antibodies and lectins are used both to capture specifically-tagged targets and for the isolation of cells and biomolecules that naturally express the ligand binding partner. The unique saccharide-binding properties of plant lectins, such as Concanavalin A (Con A) have made them useful for the labeling and isolation of glycan-presenting cells and glycoproteins in serum and cell lysate. Lectins have additionally been used in cell adhesion studies, to effect lymphocyte activation, and to explore carbohydrate-based therapeutics.. Learn More ...
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* found in: Concanavalin A (Con A) Agarose, Blue Sepharose 6 Fast Flow, Streptavidin Sepharose High Performance, Glutathione Resin, Ni-NTA Magnetic Beads,..
National Documentation Centre (EKT). National Archive of PhD Theses.PhD thesis.1986 . Creators: ΚΩΤΣΟΒΑΣΙΛΗΣ, ΚΩΝΣΤΑΝΤΙΝΟΣ.IN THE PRESENT STUDY THE DEGREE OF GLYCATION OF A1-ACID GLYCOPROTEIN (A1AGP) IN MALIGNANCY WAS INVESTIGATED BY THE METHOD OF AFFINITY IMMUNE ELECTROPHORESISIN TWO DIMENSIONS WITH CONCANAVALIN A IN THE FIRST DIMENSION. THE IMMUNOELECTROPHORETIC PATTERN OF A1AGP IN THE SERA FROM PATIENTS WITH VARIOUS NEOPLASTIC DISORDERS WAS INVESTIGATED TO DETERMINE WHETHER THE DEGREE OF GLYCATION OF A1AGP IS AFFECTED IN THE VARIOUS MALIGNANT GROUPS AND TO WHAT EXTENT THE CHANGES OBSERVED ARE CONSISTENT WITHIN A TUMOR GROUP AND CAN BE CHARACTERISTIC OF THE GROUP. THE TUMORS STUDIED WERE CLINICALLY CLASSIFIED INTO 11 GROUPS IN WHICH 8 DIFFERENT TYPES OF CHARACTERISTIC GLYCATION PATTERNS OF A1AGP HAVE BEEN RECOGNIZED. STATISTICAL ANALYSIS OF THE DATA BY CHI-SQUARE AND STUDENTS T-TEST PROVIDED FURTHER SUPPORT FOR THE SIGNIFICANCE OF THE FINDINGS ALREADY MENTIONED..
Synthesis of p-Isothiocyanatophenyl 3-O-(3,6-Dideoxy-alpha-D-xylo-hexopyranosyl)-alpha-D-mannopyranoside.. Eklind, Karin; Garegg, Per J.; Gotthammar, Birgitta ...
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Differential reactivity with concanavalin A". Biol. Chem. Hoppe-Seyler. 373 (10): 1009-18. doi:10.1515/bchm3.1992.373.2.1009. ...
... the others are concanavalin A, concanavalin B, and urease. Canavalin is a vicilin protein homologous to phaseolin. The ... CONCANAVALIN A AND CONCANAVALIN B". Science. 87 (2261): 395-396. doi:10.1126/science.87.2261.395. ISSN 0036-8075. PMID 17746464 ...
David Goodsell (2010) Concanavalin A and Circular Permutation Research Collaboratory for Structural Biology (RCSB) Protein Data ... Cunningham, B. A.; Hemperly, J. J.; Hopp, T. P.; Edelman, G. M. (1979). "Favin versus concanavalin A: Circularly permuted amino ... Bowles, D. J.; Pappin, D. J. (1988). "Traffic and assembly of concanavalin A". Trends in Biochemical Sciences. 13 (2): 60-64. ... In 1979, the first pair of circularly permuted proteins - concanavalin A and lectin - were discovered; over 2000 such proteins ...
Naismith, Jim (1992). Structural studies of Concanavalin A and zinc aldolase (PhD thesis). University of Manchester. ( ... at the University of Manchester where he was awarded a PhD in 1992 for research into the chemical structure of Concanavalin A ...
The lectin concanavalin A (conA) from jack bean is exceptional in that the two chains are transposed and ligated (by formation ... "Polypeptide ligation occurs during post-translational modification of concanavalin A". Nature. 313 (5997): 64-7. doi:10.1038/ ...
Concanavalin a and Concanavalin B". Science 87 (2261): 395-396. Bibcode:1938Sci....87..395S. PMID 17746464. doi:10.1126/science ... Interactions with concanavalin A and with Cytoplasmic structures". J Cell Biol 65 (1): 123-146. PMC 2111157. PMID 1092699. doi: ... Weiser MM (1972). "Concanavalin A agglutination of intestinal cells from the human fetus". Science 177 (4048): 525-6. Bibcode: ... Hardman KD, Ainsworth CF (1972). "Structure of concanavalin A at 2.4-A resolution". Biochemistry 11 (26): 4910-9. PMID 4638345 ...
Isolation, characterization, and preparation of cytotoxic complexes with concanavalin A. J. Biol. Chem. 255, 6947-6953 (1980). ...
Arnold HH, Heinze H (1990). "Treatment of human peripheral lymphocytes with concanavalin A activates expression of glutathione ...
Kehoe, J. (1978-08-31). "Transformation by concanavalin A of the response of molluscan neurones to L-glutamate". Nature. 274 ( ...
lectins such as Concanavalin A IMPDH and IMPDH2 Tetrameric protein "GO term: protein homotetramerization". YeastGenome. ...
Furthermore, CB can intensify pinocytosis, which is induced by concanavalin A in amoeba proteus. Cytochalasin B can also ... Prusch, RD (1981). "The Influence of Concanavalin A and Cytochalasin B on Pinocytotic Activity in Amoeba Proteus". Protoblasma ...
Zhao R, Guerrah A, Tang H, Zhao ZJ (2002). "Cell surface glycoprotein PZR is a major mediator of concanavalin A-induced cell ...
Lectins, such as concanavalin A are proteins which can bind specific alpha-D-mannose and alpha-D-glucose carbohydrate molecules ...
... and concanavalin A binding proteins of Methanosarcina acetivorans and Methanosarcina mazei". J Proteome Res. 8 (4): 1972-82. ...
"In situ generation and screening of a dynamic combinatorial carbohydrate library against concanavalin A". ChemBioChem. 1 (1): ...
... has a number of similarities to Concanavalin A and binds to mannose-related carbohydrate structures. It was discovered ...
Cytochemistry and autoradiography during stimulation by concanavalin A". Biol. Cell. 49 (1): 35-43. doi:10.1111/j.1768-322x. ...
Phospholipase A2 and cyclooxygenase blocking reduced structural abnormalities of the rat retina in concanavalin model of PVR ... "Influence of lornoxicam and triamcinolone on the dynamics of eye remodeling in concanavalin model of inflammation". Acta ... and reduced the frequency of membrane formation by 43% in the dispase model of PVR and by 31% in the concanavalin one. ...
Y Ebara and Y Okahata."A Kinetic Study of Concanavalin A Binding to Glycolipid Monolayers by Using a Quartz-Crystal ...
... s from legume plants, such as PHA or concanavalin A, have been used widely as model systems to understand the molecular ... Concanavalin A and other commercially available lectins have been used widely in affinity chromatography for purifying ... The first lectin to be purified on a large scale and available on a commercial basis was concanavalin A, which is now the most- ... Bacillus thuringiensis Concanavalin A, Phytohaemagglutinin Con A Proteopedia 1bxh, Pokeweed lectin Proteopedia 1uha, Artocarpus ...
It is also the source of concanavalin A, a lectin used in biotechnology applications, such as lectin affinity chromatography. ... It is also the source of concanavalin A. C. ensiformis is a twining plant up to 1 metre (3.3 ft) in height. It has deep roots, ...
R. Ballerstädt; R. Ehwald (1994). "Suitability of aqueous dispersions of dextran and Concanavalin A for glucose sensing in ...
Komatsu S, Yamada E, Furukawa K (January 2009). "Cold stress changes the concanavalin A-positive glycosylation pattern of ...
"Restoration of detergent-inactivated adenosine triphosphatase activity of human prostatic fluid with concanavalin A"] Check , ...
OF RETINA AT THE DEVELOPMENT OF PROLIFERATIVE VITREORETINOPATHY IN RATS AFTER INTRAOCULAR INJECTION OF CONCANAVALIN A AND ...
... goat IgG against canavalia concanavalin A, conjugated with Horseradish peroxidase \ GA/ConA/PO for more molecular products just ... Product name : goat IgG against canavalia concanavalin A, conjugated with Horseradish peroxidase ... GA/ConA/PO goat IgG against canavalia concanavalin A, conjugated with Horseradish peroxidase Ask technical file . ... We have also other products like : goat IgG against canavalia concanavalin A, conjugated with Horseradish peroxidase. Related ...
Concanavalin A at the US National Library of Medicine Medical Subject Headings (MeSH) Concanavalin A Receptors at the US ... Concanavalin a and Concanavalin B". Science. 87 (2261): 395-6. Bibcode:1938Sci....87..395S. doi:10.1126/science.87.2261.395. ... Concanavalin A has a low-frequency wave number of 20 cm−1 in its Raman spectra. This emission has been assigned to the ... Interactions with concanavalin A and with Cytoplasmic structures". The Journal of Cell Biology. 65 (1): 123-46. doi:10.1083/jcb ...
concanavalin synonyms, concanavalin pronunciation, concanavalin translation, English dictionary definition of concanavalin. n ... concanavalin B or concanavalin A, that occur in the jack bean Canavalia ensiformis . Concanavalin A causes agglutination of the ... concanavalin. Also found in: Medical, Wikipedia. concanavalin. (ˌkɒnkəˈnævəlɪn) n. either of two globulins, concanavalin B or ( ... Concanavalin - definition of concanavalin by The Free Dictionary https://www.thefreedictionary.com/concanavalin ...
... concanavalin A (our unpublished results), pokeweed mitogen and mercurous chloride3. At optimum ratios of mitogen to serum most ... Inhibition of Lymphocyte Activation at High Ratios of Concanavalin A to Serum depends on Complement. *PETER MILTHORP1. & ... MILTHORP, P., FORSDYKE, D. Inhibition of Lymphocyte Activation at High Ratios of Concanavalin A to Serum depends on Complement ... concanavalin A (our unpublished results), pokeweed mitogen and mercurous chloride3. At optimum ratios of mitogen to serum most ...
The intravenous injection of the plant lectin concanavalin A (ConA) is a widely used model for acute immune-mediated hepatitis ... The Concanavalin A Model of Acute Hepatitis in Mice Lab Anim. 2015 Apr;49(1 Suppl):12-20. doi: 10.1177/0023677215572841. ... The intravenous injection of the plant lectin concanavalin A (ConA) is a widely used model for acute immune-mediated hepatitis ...
Peroxidase labeled concanavalin A (Con A) permits the detection of some saccharide determinants. This histochemical technique ... 11028-71-0/Concanavalin A; 58-60-6/Puromycin Aminonucleoside From MEDLINE®/PubMed®, a database of the U.S. National Library of ... Concanavalin A / diagnostic use*. Cytoplasm / ultrastructure. Endoplasmic Reticulum / analysis, ultrastructure. ... Peroxidase labeled concanavalin A (Con A) permits the detection of some saccharide determinants. This histochemical technique ...
Concanavalin A prevents acetylcholine receptor redistribution in Xenopus nerve-muscle cultures. Y Kidokoro, B Brass and H ... Concanavalin A prevents acetylcholine receptor redistribution in Xenopus nerve-muscle cultures Message Subject (Your Name) has ... If this were the major mechanism, cross-linking ACh receptors by tetravalent concanavalin A (Con A) should immobilize receptors ...
Rabbit Ileal Lamina Propria (LP) Lymphocytes Give Poor Blastogenic Responses to KLH Immunization and Concanavalin a Mitogenesis ... proliferative response to Concanavalin A (Con A) mitogen stimulation and KLH immunization by lamina propria (LP) and Peyers ... Lymphocytes Give Poor Blastogenic Responses to KLH Immunization and Concanavalin a Mitogenesis. In: Mestecky J., McGhee J.R., ...
... Tong Liu,1 Yujing Xia, ... Concanavalin A (ConA) has the ability to stimulate the activation of T lymphocytes, mostly CD4+ T-cells, leading to immune ... H. X. Wang, M. Liu, S.-Y. Weng et al., "Immune mechanisms of Concanavalin A model of autoimmune hepatitis," World Journal of ... H. X. Wang, M. Liu, S.-Y. Weng et al., "Immune mechanisms of Concanavalin A model of autoimmune hepatitis," World Journal of ...
... Tong Liu,1 Yujing Xia, ... H. X. Wang, M. Liu, S.-Y. Weng et al., "Immune mechanisms of Concanavalin A model of autoimmune hepatitis," World Journal of ... H. X. Wang, M. Liu, S.-Y. Weng et al., "Immune mechanisms of Concanavalin A model of autoimmune hepatitis," World Journal of ... J. H. Lee, J. H. Won, J. M. Choi et al., "Protective effect of ellagic acid on concanavalin A-induced hepatitis via toll-like ...
Effects of Concanavalin A on Cytokine mRNA Expression in Mouse Liver * * Okamoto Toshihiro OKAMOTO Toshihiro ... The effects of concanavalin A (Con A) on liver cytokine gene expression was studied in mice. The CD4 mRNA expression in normal ... Concanavalin A-induced T-cell-mediated hepatic injury in mice. The role of tumor necrosis factor GANTNER F. ... Effects of Concanavalin A Treatment on CD8 mRNA Expression in Mouse Liver Okamoto Toshihiro , Nakano Yoshihisa , Tsuzuike Naoki ...
Abstract Concanavalin A (Con A) agglutinated all Naegleria gruberi strains tested but did not agglutinate any N. fowleri ... Concanavalin A-Induced Agglutination of Naegleria * S. L. Josephson, R. R. Weik, D. T. John ... Concanavalin A (Con A) agglutinated all Naegleria gruberi strains tested but did not agglutinate any N. fowleri strains tested ...
More info for Superfamily b.29.1: Concanavalin A-like lectins/glucanases. Timeline for Superfamily b.29.1: Concanavalin A-like ... Superfamily b.29.1: Concanavalin A-like lectins/glucanases appears in SCOPe 2.01. *Superfamily b.29.1: Concanavalin A-like ... Lineage for Superfamily b.29.1: Concanavalin A-like lectins/glucanases. *Root: SCOPe 2.02 *. Class b: All beta proteins [48724 ... Fold b.29: Concanavalin A-like lectins/glucanases [49898] (1 superfamily). sandwich; 12-14 strands in 2 sheets; complex ...
This green-fluorescent Alexa Fluor 488 conjugate of succinylated concanavalin A (Con A) selectively binds to -mannopyranosyl ... This green-fluorescent Alexa Fluor 488 conjugate of succinylated concanavalin A (Con A) selectively binds to α-mannopyranosyl ...
Therapeutic advantage of anti-VAP-1 over anti-α4 integrin antibody in concanavalin a-induced hepatitis. ... Therapeutic advantage of anti-VAP-1 over anti-α4 integrin antibody in concanavalin a-induced hepatitis. Hepatology, 58: 1413- ...
Dietary phosphatidylinositol protects C57BL/6 mice from concanavalin A-induced liver injury by modulating immune cell functions ... Dietary phosphatidylinositol protects C57BL/6 mice from concanavalin A-induced liver injury by modulating immune cell functions ...
Upon induction of acute hepatitis using concanavalin A (ConA), down-regulation of the in vivo imaging signal obtained using ... Monitoring liver macrophages using nanobodies targeting Vsig4: concanavalin A induced acute hepatitis as paradigm.. [Fang Zheng ...
In this study, we investigated the protective effects of EGCG in concanavalin A (ConA)-induced hepatitis in mice and explored ... Epigallocatechin-3-gallate attenuates apoptosis and autophagy in concanavalin A-induced hepatitis by inhibiting BNIP3.. [Sainan ...
A structure of the complex between concanavalin A and methyl-3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside reveals ... CO-CRYSTALS OF CONCANAVALIN A WITH METHYL-3,6-DI-O-(ALPHA-D-MANNOPYRANOSYL)-ALPHA-D-MANNOPYRANOSIDE. ...
... against concanavalin A (Con A)-induced immunological hepatic injury in mice. In this study, we investigated the ... We have previously demonstrated the hepatoprotective effect of dihydroquercetin (DHQ) against concanavalin A (Con A)-induced ... Pretreatment with dihydroquercetin, a dietary flavonoid, protected against concanavalin A-induced immunological hepatic injury ... Pretreatment with dihydroquercetin, a dietary flavonoid, protected against concanavalin A-induced immunological hepatic injury ...
Concanavalin A (ConA), a plant lectin from jack beans, is known to mitogenically activate T lymphocytes via the antigen ... Effect of concanavalin A (ConA) treatment is shown. Control, naive T lymphocytes from intestinal lymph. ConA, T cells ... Altered migration of gut-derived T lymphocytes after activation with concanavalin A. Ryota Hokari, Soichiro Miura, Hitoshi ... The objectives of this study were1) to compare migration of naive and concanavalin A (ConA)-activated T lymphocytes into the ...
Dissociation constant for binding affinity towards Concanavalin A determined by fluorescence anisotropy inhibition assay. ...
Purification of concanavalin A receptor from pig lymphocyte plasma membrane. D Allan, J Auger, M J Crumpton ... Purification of concanavalin A receptor from pig lymphocyte plasma membrane Message Subject (Your Name) has forwarded a page to ...
effect during the binding of concanavalin A to mannosylated artificial lipid. rafts G. T. Noble, S. L. Flitsch, K. P. Liem and ... effect during the binding of concanavalin A to mannosylated artificial lipid. rafts ...
Concanavalin A induced 29 to 46% of the initial population to enter proliferation over 1 to 5 days. In mixed leukocyte cultures ... Mouse lymphocytes were cultured with phytohemagglutinin, concanavalin A, or allogeneic cells. The number of cells entering DNA ... The Number of Reactive Cells in Mouse Lymphocyte Cultures Stimulated by Phytohemagglutinin, Concanavalin a or ... The Number of Reactive Cells in Mouse Lymphocyte Cultures Stimulated by Phytohemagglutinin, Concanavalin a or ...
  • 1. The luminol-dependent chemiluminescence of rat thymocytes responding to concanavalin A can be resolved into glucose-dependent and glucose-independent portions. (biochemj.org)
  • 4. Preincubation of thymocytes for 18 h in serum-free medium enhances the subsequent chemiluminescent response to concanavalin A severalfold and evokes the response at a lower threshold concentration. (biochemj.org)
  • El Bawab S, Macovschi O, Thevenon C, Goncalves A, Nemoz G, Lagarde M, Prigent A-F (1996) Contribution of phosphoinositides and phosphatidylcholines to the production of phosphatidic acid upon concanavalin A stimulation of rat thymocytes. (springer.com)
  • Highly sensitive biosensing of concanavalin A (Con A) has been developed based on the attenuation of FRET efficiency between amine-terminated quantum dots and mannose-stabilized Au nanoparticles in the presence of Con A, which inhibits the H-bonding formation of the QDs-AuNPs assembly, thereby causing the fluorescence recovery. (elsevier.com)
  • Lim, KR, Ahn, KS & Lee, WY 2013, ' Detection of concanavalin A based on attenuated fluorescence resonance energy transfer between quantum dots and mannose-stabilized gold nanoparticles ', Analytical Methods , vol. 5, no. 1, pp. 64-67. (elsevier.com)
  • 1) John L. Wang, Bruce A. Cunningham and Gerald M. Edelman (1971) Unusual Fragments in the Subunit Structure of Concanavalin A (gelelectrophoresis/molecularweights) Proc. (medicago.se)
  • Haptoglobin binding to Concanavalin A, immobilized to polystyrene microtiter plate, was estimated by anti-haptoglobin polyclonal and monoclonal antibodies conjugated with horse-radish peroxidase. (docphin.com)
  • Thus concanavalin A may activate, transiently, an NAD(P)H oxidase that utilizes reducing equivalents derived from the oxidation of exogenous glucose to give dismutation products of O2- (including H2O2) as its major products. (biochemj.org)
  • Concanavalin A (Con A) was fragmented by a proteolytic process and the m-Con A) derivative was isolated by elution with an ascending D-glucose gradient on a Sephadex G-200 column. (mysciencework.com)
  • Oral administration of the 50% ethanol extract of melinjo fruit at 100 mg/kg/day significantly enhanced the production of the Th1 cytokines IL-2 and IFN-γ irrespective of concanavalin-A stimulation, whereas the production of the Th2 cytokines IL-4 and IL-5 was not affected. (wikipedia.org)
  • He won a Carnegie Scholarship to work under the supervision of Bill Hunter, John Helliwell and David Garner at the University of Manchester where he was awarded a PhD in 1992 for research into the chemical structure of Concanavalin A and Zinc aldolase. (wikipedia.org)