Complementarity Determining Regions
Three regions (CDR1; CDR2 and CDR3) of amino acid sequence in the IMMUNOGLOBULIN VARIABLE REGION that are highly divergent. Together the CDRs from the light and heavy immunoglobulin chains form a surface that is complementary to the antigen. These regions are also present in other members of the immunoglobulin superfamily, for example, T-cell receptors (RECEPTORS, ANTIGEN, T-CELL).
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Immunoglobulin Variable Region
That region of the immunoglobulin molecule that varies in its amino acid sequence and composition, and comprises the binding site for a specific antigen. It is located at the N-terminus of the Fab fragment of the immunoglobulin. It includes hypervariable regions (COMPLEMENTARITY DETERMINING REGIONS) and framework regions.
Immunoglobulin Heavy Chains
Nucleic Acid Conformation
Amino Acid Sequence
RNA, Small Nuclear
Short chains of RNA (100-300 nucleotides long) that are abundant in the nucleus and usually complexed with proteins in snRNPs (RIBONUCLEOPROTEINS, SMALL NUCLEAR). Many function in the processing of messenger RNA precursors. Others, the snoRNAs (RNA, SMALL NUCLEOLAR), are involved with the processing of ribosomal RNA precursors.
Binding Sites, Antibody
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
RNA, Ribosomal, 18S
A measure of the binding strength between antibody and a simple hapten or antigen determinant. It depends on the closeness of stereochemical fit between antibody combining sites and antigen determinants, on the size of the area of contact between them, and on the distribution of charged and hydrophobic groups. It includes the concept of "avidity," which refers to the strength of the antigen-antibody bond after formation of reversible complexes.
Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.
Immunoglobulin Light Chains
Immunoglobulin Fab Fragments
Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.
3' Untranslated Regions
RNA, Ribosomal, 28S
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.
5' Untranslated Regions
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Receptors, Antigen, T-Cell, alpha-beta
Genes encoding the different subunits of the IMMUNOGLOBULINS, for example the IMMUNOGLOBULIN LIGHT CHAIN GENES and the IMMUNOGLOBULIN HEAVY CHAIN GENES. The heavy and light immunoglobulin genes are present as gene segments in the germline cells. The completed genes are created when the segments are shuffled and assembled (B-LYMPHOCYTE GENE REARRANGEMENT) during B-LYMPHOCYTE maturation. The gene segments of the human light and heavy chain germline genes are symbolized V (variable), J (joining) and C (constant). The heavy chain germline genes have an additional segment D (diversity).
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Gene Rearrangement, B-Lymphocyte, Heavy Chain
The phenomenon of immense variability characteristic of ANTIBODIES. It enables the IMMUNE SYSTEM to react specifically against the essentially unlimited kinds of ANTIGENS it encounters. Antibody diversity is accounted for by three main theories: (1) the Germ Line Theory, which holds that each antibody-producing cell has genes coding for all possible antibody specificities, but expresses only the one stimulated by antigen; (2) the Somatic Mutation Theory, which holds that antibody-producing cells contain only a few genes, which produce antibody diversity by mutation; and (3) the Gene Rearrangement Theory, which holds that antibody diversity is generated by the rearrangement of IMMUNOGLOBULIN VARIABLE REGION gene segments during the differentiation of the ANTIBODY-PRODUCING CELLS.
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
A field of biology concerned with the development of techniques for the collection and manipulation of biological data, and the use of such data to make biological discoveries or predictions. This field encompasses all computational methods and theories for solving biological problems including manipulation of models and datasets.
RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Protein Structure, Secondary
The most abundant form of RNA. Together with proteins, it forms the ribosomes, playing a structural role and also a role in ribosomal binding of mRNA and tRNAs. Individual chains are conventionally designated by their sedimentation coefficients. In eukaryotes, four large chains exist, synthesized in the nucleolus and constituting about 50% of the ribosome. (Dorland, 28th ed)
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
RNA, Transfer, Lys
Multicellular, eukaryotic life forms of kingdom Plantae (sensu lato), comprising the VIRIDIPLANTAE; RHODOPHYTA; and GLAUCOPHYTA; all of which acquired chloroplasts by direct endosymbiosis of CYANOBACTERIA. They are characterized by a mainly photosynthetic mode of nutrition; essentially unlimited growth at localized regions of cell divisions (MERISTEMS); cellulose within cells providing rigidity; the absence of organs of locomotion; absence of nervous and sensory systems; and an alternation of haploid and diploid generations.
Sequence Homology, Amino Acid
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
Sequence Analysis, RNA
Sequence Homology, Nucleic Acid
Immunoglobulin Joining Region
A segment of the immunoglobulin heavy chains, encoded by the IMMUNOGLOBULIN HEAVY CHAIN GENES in the J segment where, during the maturation of B-LYMPHOCYTES; the gene segment for the variable region upstream is joined to a constant region gene segment downstream. The exact position of joining of the two gene segments is variable and contributes to ANTIBODY DIVERSITY. It is distinguished from the IMMUNOGLOBULIN J CHAINS; a separate polypeptide that serves as a linkage piece in polymeric IGA or IGM.
Polymerase Chain Reaction
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
RNA Splice Sites
RNA, Small Nucleolar
Small nuclear RNAs that are involved in the processing of pre-ribosomal RNA in the nucleolus. Box C/D containing snoRNAs (U14, U15, U16, U20, U21 and U24-U63) direct site-specific methylation of various ribose moieties. Box H/ACA containing snoRNAs (E2, E3, U19, U23, and U64-U72) direct the conversion of specific uridines to pseudouridine. Site-specific cleavages resulting in the mature ribosomal RNAs are directed by snoRNAs U3, U8, U14, U22 and the snoRNA components of RNase MRP and RNase P.
RNA, Small Untranslated
Unique genetically-controlled determinants present on ANTIBODIES whose specificity is limited to a single group of proteins (e.g., another antibody molecule or an individual myeloma protein). The idiotype appears to represent the antigenicity of the antigen-binding site of the antibody and to be genetically codetermined with it. The idiotypic determinants have been precisely located to the IMMUNOGLOBULIN VARIABLE REGION of both immunoglobin polypeptide chains.
Nucleic Acid Hybridization
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Nucleic acid structures found on the 5' end of eukaryotic cellular and viral messenger RNA and some heterogeneous nuclear RNAs. These structures, which are positively charged, protect the above specified RNAs at their termini against attack by phosphatases and other nucleases and promote mRNA function at the level of initiation of translation. Analogs of the RNA caps (RNA CAP ANALOGS), which lack the positive charge, inhibit the initiation of protein synthesis.
Surface Plasmon Resonance
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
A gene silencing phenomenon whereby specific dsRNAs (RNA, DOUBLE-STRANDED) trigger the degradation of homologous mRNA (RNA, MESSENGER). The specific dsRNAs are processed into SMALL INTERFERING RNA (siRNA) which serves as a guide for cleavage of the homologous mRNA in the RNA-INDUCED SILENCING COMPLEX. DNA METHYLATION may also be triggered during this process.
Protein Structure, Quaternary
RNA-Induced Silencing Complex
A multicomponent, ribonucleoprotein complex comprised of one of the family of ARGONAUTE PROTEINS and the "guide strand" of the one of the 20- to 30-nucleotide small RNAs. RISC cleaves specific RNAs, which are targeted for degradation by homology to these small RNAs. Functions in regulating gene expression are determined by the specific argonaute protein and small RNA including siRNA (RNA, SMALL INTERFERING), miRNA (MICRORNA), or piRNA (PIWI-INTERACTING RNA).
Gene Rearrangement, beta-Chain T-Cell Antigen Receptor
RNA Processing, Post-Transcriptional
Receptors, Antigen, T-Cell
Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.
A group of genetically identical cells all descended from a single common ancestral cell by mitosis in eukaryotes or by binary fission in prokaryotes. Clone cells also include populations of recombinant DNA molecules all carrying the same inserted sequence. (From King & Stansfield, Dictionary of Genetics, 4th ed)
Conservation of Natural Resources
Within most types of eukaryotic CELL NUCLEUS, a distinct region, not delimited by a membrane, in which some species of rRNA (RNA, RIBOSOMAL) are synthesized and assembled into ribonucleoprotein subunits of ribosomes. In the nucleolus rRNA is transcribed from a nucleolar organizer, i.e., a group of tandemly repeated chromosomal genes which encode rRNA and which are transcribed by RNA polymerase I. (Singleton & Sainsbury, Dictionary of Microbiology & Molecular Biology, 2d ed)
Nucleic Acid Denaturation
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
Hydrophobic and Hydrophilic Interactions
Nucleic Acid Heteroduplexes
Double-stranded nucleic acid molecules (DNA-DNA or DNA-RNA) which contain regions of nucleotide mismatches (non-complementary). In vivo, these heteroduplexes can result from mutation or genetic recombination; in vitro, they are formed by nucleic acid hybridization. Electron microscopic analysis of the resulting heteroduplexes facilitates the mapping of regions of base sequence homology of nucleic acids.
RNA consisting of two strands as opposed to the more prevalent single-stranded RNA. Most of the double-stranded segments are formed from transcription of DNA by intramolecular base-pairing of inverted complementary sequences separated by a single-stranded loop. Some double-stranded segments of RNA are normal in all organisms.
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A set of three nucleotides in a protein coding sequence that specifies individual amino acids or a termination signal (CODON, TERMINATOR). Most codons are universal, but some organisms do not produce the transfer RNAs (RNA, TRANSFER) complementary to all codons. These codons are referred to as unassigned codons (CODONS, NONSENSE).
Base Pair Mismatch
The presence of an uncomplimentary base in double-stranded DNA caused by spontaneous deamination of cytosine or adenine, mismatching during homologous recombination, or errors in DNA replication. Multiple, sequential base pair mismatches lead to formation of heteroduplex DNA; (NUCLEIC ACID HETERODUPLEXES).
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
Ribonucleoproteins, Small Nuclear
Sequence Analysis, DNA
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 22.214.171.124.
RNA that has catalytic activity. The catalytic RNA sequence folds to form a complex surface that can function as an enzyme in reactions with itself and other molecules. It may function even in the absence of protein. There are numerous examples of RNA species that are acted upon by catalytic RNA, however the scope of this enzyme class is not limited to a particular type of substrate.
A theoretical representative nucleotide or amino acid sequence in which each nucleotide or amino acid is the one which occurs most frequently at that site in the different sequences which occur in nature. The phrase also refers to an actual sequence which approximates the theoretical consensus. A known CONSERVED SEQUENCE set is represented by a consensus sequence. Commonly observed supersecondary protein structures (AMINO ACID MOTIFS) are often formed by conserved sequences.
The molecular designing of drugs for specific purposes (such as DNA-binding, enzyme inhibition, anti-cancer efficacy, etc.) based on knowledge of molecular properties such as activity of functional groups, molecular geometry, and electronic structure, and also on information cataloged on analogous molecules. Drug design is generally computer-assisted molecular modeling and does not include pharmacokinetics, dosage analysis, or drug administration analysis.
Peptide Chain Initiation, Translational
A process of GENETIC TRANSLATION whereby the formation of a peptide chain is started. It includes assembly of the RIBOSOME components, the MESSENGER RNA coding for the polypeptide to be made, INITIATOR TRNA, and PEPTIDE INITIATION FACTORS; and placement of the first amino acid in the peptide chain. The details and components of this process are unique for prokaryotic protein biosynthesis and eukaryotic protein biosynthesis.
Repetitive Sequences, Nucleic Acid
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Amino Acid Motifs
Genetic Complementation Test
The small RNA molecules, 73-80 nucleotides long, that function during translation (TRANSLATION, GENETIC) to align AMINO ACIDS at the RIBOSOMES in a sequence determined by the mRNA (RNA, MESSENGER). There are about 30 different transfer RNAs. Each recognizes a specific CODON set on the mRNA through its own ANTICODON and as aminoacyl tRNAs (RNA, TRANSFER, AMINO ACYL), each carries a specific amino acid to the ribosome to add to the elongating peptide chains.
Elements that are transcribed into RNA, reverse-transcribed into DNA and then inserted into a new site in the genome. Long terminal repeats (LTRs) similar to those from retroviruses are contained in retrotransposons and retrovirus-like elements. Retroposons, such as LONG INTERSPERSED NUCLEOTIDE ELEMENTS and SHORT INTERSPERSED NUCLEOTIDE ELEMENTS do not contain LTRs.
Viruses which lack a complete genome so that they cannot completely replicate or cannot form a protein coat. Some are host-dependent defectives, meaning they can replicate only in cell systems which provide the particular genetic function which they lack. Others, called SATELLITE VIRUSES, are able to replicate only when their genetic defect is complemented by a helper virus.
Magnetic Resonance Spectroscopy
Characterization of T-cell repertoire of the bone marrow in immune-mediated aplastic anemia: evidence for the involvement of antigen-driven T-cell response in cyclosporine-dependent aplastic anemia. (1/723)To determine whether the antigen-driven T-cell response is involved in the pathogenesis of aplastic anemia (AA), we examined the complementarity-determining region 3 (CDR3) size distribution of T-cell receptor (TCR) beta-chain (BV) subfamilies in the bone marrow (BM) of untreated AA patients. AA patients who did not respond to immunosuppressive therapy and those who obtained unmaintained remission early after cyclosporine (CyA) or antithymocyte globulin (ATG) therapy exhibited essentially a normal CDR3 size pattern. In contrast, five patients who needed continuous administration of CyA to maintain remission exhibited a skewed CDR3 size pattern in a number (>40%) of BV subfamilies suggestive of clonal predominance. The skewing of CDR3 size distribution became less pronounced in one of the CyA-dependent patients when the patient achieved unmaintained remission after a 4-year therapy with CyA, whereas it persisted longer than 7 years in the other patient requiring maintenance therapy. Sequencing of BV15 cDNA for which the CDR3 size pattern exhibited apparent clonal predominance in all CyA-dependent patients showed high homology of the amino acid sequence of the CDR3 between two different patients. These findings indicate that antigen-driven expansion of T cells is involved in the pathogenesis of AA characterized by CyA-dependent recovery of hematopoiesis. (+info)
A peptide derived from a polyreactive monoclonal anti-DNA natural antibody can modulate lupus development in (NZBxNZW)F1 mice. (2/723)In lupus-prone (NZBxNZW)F1 (B/W) mice, elevated levels of polyreactive autoantibodies bearing the D23 idiotype (Id), characteristic of natural antibodies, were detected before and after the appearance of pathological anti-DNA antibodies. While these D23 Id+ antibodies were able to regulate anti-DNA antibodies in the early stage of the disease, we found that during disease evolution they had lost their normal ability to regulate anti-DNA antibodies and furthermore could participate in the lupus-like syndrome. To explore further the role of the D23 Id+ antibodies, we injected young B/W mice with a peptide corresponding to the VH CDR3 region of the D23 monoclonal natural antibody (mNAb). High levels of monospecific antipeptide, as well as polyreactive antibodies, were induced. Among them, the most markedly enhanced antibody population was DNA-reactive immunoglobulin G1 (IgG1). Compared with controls, these immunized mice had a delayed 50% survival rate and proteinuria developed later. Furthermore, IgG1 able to react with IgG2a anti-DNA monoclonal antibodies derived from B/W mice were also produced after peptide immunization. Thus, a peptide corresponding to the CDR3 of the D23 mNAb antibody might play a role in the regulation of murine lupus. (+info)
Polyclonal expansion of TCRBV2- and TCRBV6-bearing T cells in patients with Kawasaki disease. (3/723)We examined T-cell receptor (TCR) usage, cytokine production and antibody responses to superantigens in patients with Kawasaki disease (KD) to facilitate a better understanding of the immunopathogenesis of KD. The mean percentage of VB2- or VB6. 5-bearing T cells in peripheral blood mononuclear cells (PBMC) of patients with acute-phase KD was significantly higher than that of patients in the convalescent phase of KD or in healthy donors. Expansion of VB2- or VB6.5-bearing T cells was polyclonal because DNA sequences in the complementarity determining region 3 of VB2- and VB6.5-positive cDNA clones were all different from each other. The plasma levels of interleukin (IL)-1beta, IL-2, IL-6, IL-8, IL-10, interferon-gamma (IFN-gamma), tumour necrosis factor-alpha (TNF-alpha) and granulocyte colony-stimulating factor (G-CSF) were elevated in the acute phase of KD. We previously reported that streptococcal pyrogenic exotoxin C (SPEC) was a potent stimulator of VB2- and VB6.5-positive T cells and, furthermore, serum levels of anti-SPEC antibodies were significantly higher in patients with acute and convalescent KD than in age-matched controls. The results of the present study, together with those of our previous report, suggest that SPEC induces activation and polyclonal expansion of VB2- and VB6.5-positive T cells, and that SPEC-induced activation of T cells may lead to the pathogenesis of KD. (+info)
Evolution of antigen-specific T cell receptors in vivo: preimmune and antigen-driven selection of preferred complementarity-determining region 3 (CDR3) motifs. (4/723)Antigen (Ag)-driven selection of helper T cells (Th) in normal animals has been difficult to study and remains poorly understood. Using the major histocompatibility complex class II- restricted murine response to pigeon cytochrome c (PCC), we provide evidence for both preimmune and Ag-driven selection in the evolution of Ag-specific immunity in vivo. Before antigenic challenge, most Valpha11(+)Vbeta3(+) Th (70%) express a critical complementarity-determining region 3 (CDR3) residue (glutamic acid at TCR-alpha93) associated with PCC peptide contact. Over the first 5 d of the primary response, PCC-responsive Valpha11(+)Vbeta3(+) Th expressing eight preferred CDR3 features are rapidly selected in vivo. Clonal dominance is further propagated through selective expansion of the PCC-specific cells with T cell receptor (TCR) of the "best fit." Ag-driven selection is complete before significant emergence of the germinal center reaction. These data argue that thymic selection shapes TCR-alpha V region bias in the preimmune repertoire; however, Ag itself and the nongerminal center microenvironment drive the selective expansion of clones with preferred TCR that dominate the response to Ag in vivo. (+info)
Composite low grade B-cell lymphomas with two immunophenotypically distinct cell populations are true biclonal lymphomas. A molecular analysis using laser capture microdissection. (5/723)Low grade B-cell lymphomas comprise several well defined, clinically and immunophenotypically distinct disease entities. Composite lymphomas showing phenotypic characteristics of more than one of these tumor subtypes in the same site are rare, and both common and separate clonal origins of the two tumor parts have been reported for cases studied by molecular methods. We describe the detailed immunohistochemical and molecular findings in three cases with features of composite low grade B-cell non-Hodgkin's lymphoma (B-NHL). All three neoplasms contained morphologically distinct but interwoven compartments of different cell types, which exhibited discordant expression of several markers, including CD5, CD10, CD43, and cyclin D1. According to their morphology and phenotypes, they were classified as mantle cell lymphoma and follicular lymphoma (Case 1), follicular lymphoma and small lymphocytic lymphoma (Case 2), and mantle cell lymphoma and chronic lymphocytic leukemia/small lymphocytic lymphoma (Case 3). PCR analysis of DNA obtained from whole tissue sections failed to reveal evidence for biclonality in any of the cases. We therefore isolated cell populations with different antigen expression patterns by laser capture microdissection and analyzed them by polymerase chain reaction amplification and sequencing of clonal immunoglobulin heavy chain gene rearrangements and oncogene rearrangements. Sequence analysis revealed unrelated clonal rearrangements in each of the two tumor parts in all three cases, suggesting distinct clonal origins. In addition, Case 1 showed a bcl-2 rearrangement present only in the follicular lymphoma part. Our findings suggest that low grade B-NHL with two distinct morphological and immunophenotypic patterns in the same anatomical site are frequently biclonal. This is in keeping with current classification schemes, which recognize subtypes of low grade B-NHL as separate disease entities. Furthermore, our analysis demonstrates the power of laser capture microdissection in revealing molecular microheterogeneity in complex neoplasms. (+info)
Astrocytoma infiltrating lymphocytes include major T cell clonal expansions confined to the CD8 subset. (6/723)Anaplastic astrocytoma and glioblastoma are frequent and malignant brain tumors that are infiltrated by T lymphocytes. Whether these cells result from non-specific inflammation following blood-brain barrier disruption or an antigen-driven specific immune response is unknown. In this study, an in-depth characterization of TCR diversity in tumor and blood RNA biopsies was performed in a series of 16 patients with malignant astrocytoma. Whilst there was no obvious restriction of the AV and BV gene segment usage, complementarity-determining region 3 size analysis and sequencing of amplified TCR transcripts revealed multiple T cell oligoclonal expansions in all astrocytomas analyzed. Unique T cell clones were present in different adjacent areas of a given tumor, but never detected in the blood. Quantification of the number of TCR clonal transcripts per microg of tumor RNA indicated that certain T cell clonal expansions may represent at least 300 cells/10(6) tumor cells. Furthermore, we demonstrated that the in vivo expanded clones were almost exclusively confined to the CD8(+) subset. Overall, these data suggest that spontaneous antigen-driven immune responses may be elicited against human astrocytoma despite the immunosuppressive microenvironment generated by the brain and the tumor itself. However, the ultimate failure of the immune system to control tumor growth could be the consequence of a deficient CD4 T(h) component of the response. This observation could have important consequences for the development of immunotherapies for astrocytoma patients. (+info)
Characterisation of T cell clonotypes that accumulated in multiple joints of patients with rheumatoid arthritis. (7/723)OBJECTIVE: To investigate whether identical T cell clonotypes accumulate in multiple rheumatoid joints, the clonality of T cells that had infiltrated into synovial tissue (ST) samples simultaneously obtained from multiple joints of patients with rheumatoid arthritis (RA) was analysed. METHODS: T cell receptor (TCR) beta gene transcripts, amplified by reverse transcription-polymerase chain reaction from ST and peripheral blood lymphocytes of five RA patients, were subjected to single strand conformation polymorphism analysis and DNA sequencing. RESULTS: Approximately 40% of accumulated T cell clonotypes found in one joint of a patient were found in multiple joints in the same patient. Furthermore, identical amino acid sequences were found in TCR beta junctional regions of these clonotypes from different patients with at least one HLA molecule match. CONCLUSIONS: The T cell clonotypes accumulating in multiple rheumatoid joints may be involved in the perpetuation of polyarthritis by reacting to antigens common to these multiple joints. (+info)
Selection at multiple checkpoints focuses V(H)12 B cell differentiation toward a single B-1 cell specificity. (8/723)Phosphatidyl choline (PtC)-specific B cells segregate to the B-1 subset, where they comprise up to 10% of the B-1 repertoire. About half express V(H)12 and Vkappa4/5H and are restricted in V(H)CDR3. We have previously reported that anti-PtC V(H)CDR3 is enriched among V(H)12-expressing cells by selective elimination of pre-B cells. We report here a bias for Vkappa4/5H expression among V(H)12-expressing B cells, even among those that do not bind PtC and are not B-1. This is due in part to an inability of V(H)12 to associate with many light (L) chains but must also be due to a selective advantage in survival or clonal expansion in the periphery for Vkappa4/5H-expressing cells. Thus, the bias for Vkappa4/5H expression is independent of PtC binding, and, as segregation to B-1 occurs after Ig gene expression, it precedes segregation to the B-1 subset. In 6-1 mice, splenic B-1 cells reside in follicles but segregate to follicles distinct from those that contain B-2 cells. These data indicate that selection at multiple developmental checkpoints ensures the co-expression of an anti-PtC V(H)CDR3 and L chain in a high frequency of V(H)12 B cells. This focus toward specificity for PtC facilitates the development of a large anti-PtC B-1 repertoire. (+info)
Artificial antibody created by conformational reconstruction of the complementary-determining region on gold nanoparticles |...
Here, we demonstrate that the active conformation of the CDR loops of natural antibodies can be reconstructed on AuNPs to create Goldbodies, which bind the corresponding antigens specifically with apparent affinities several orders of magnitude stronger than those of the original natural antibodies. Up to now, both NP functionalization (4⇓⇓⇓-8) and protein mimicking (48) have relied on the incorporation of functional groups. Herein we show that new functions of NPs can be created by controlling the conformation of the surface groups on NPs. Self-assembled monolayers on gold surfaces have shown certain molecular density effects on the packing of the immobilized groups (49⇓-51), yet those self-assembling methods aim to create certain macro patterns consisting of numerous chains. Precisely manipulating conformations of individual groups for specific functions is still beyond reach. The seemingly insurmountable task of tuning the conformation of individual peptides for specific ...
The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing...
The target of human immunodeficiency virus type 1 (HIV-1) neutralizing antibodies (Abs) is the putative trimer of gp120-gp41 heterodimers that decorates the surface of HIV-1 (10, 28, 43, 52, 54). In the case of gp41, it appears that antibody access to neutralizing epitopes may be more restricted than access to those on gp120, since the relevant epitopes on gp41 probably become fully exposed only during HIV-1 envelope-mediated virus-cell membrane fusion (4, 19, 20, 46). The two anti-gp41 monoclonal Abs (MAbs) that are the most potent and broadly neutralizing are the human immunoglobulin G (IgG) MAbs 2F5 and 4E10 (12, 14, 16, 21, 47, 49, 58). The core epitope of 2F5, the most studied of the two MAbs, has been defined conveniently by a short linear sequence, ELDKWA, which is found at the extreme C-terminal end of the C-heptad repeat region on the ectodomain of gp41 (37). MAb 4E10 appears to recognize an epitope immediately C-terminal to the 2F5 epitope. The 4E10 epitope has been defined by the ...
A Study to Evaluate the Effect of Fingolimod on Newly Produced T and B Lymphocytes and T-Cell Receptor Repertoire Diversity in...
This study investigated whether fingolimod reduced newly produced T and B lymphocytes and T-cell receptor repertoire diversity in peripheral blood in patients
BiBiServ2 - TCRProfiler
Next-generation sequencing enabled T-cell receptor (TCR) repertoire analysis has gained major attention in scientific as well as clinically driven research. However, despite improvements of sequencing technology, next-generation sequencers produce huge data sets that are usually prone to errors, thus urging the need for improved analysis tools being able to account not only for the large number of sequences but also for sequencing artifacts. TCRProfiler is a tool that includes sequencer generated quality values to improve reliability of TCR repertoire analysis. As a stand alone tool for parallel and detailed analysis, TCRProfiler delimits combinatorial diversity (rearranged germline V, (D), and J genes) and junctional diversity (P(alindromic)-, and N(on-template)-nucleotides) of TCR alpha and/or beta chain sequences. TCRProfiler generates probe-specific statistical repertoire profiles, as well as visualization files to allow fast and comprehensive accession of repertoire diversity. In a single ...
Immunologic perturbations in severe COVID-19/SARS-CoV-2 infection - Immunology
Novelty: Unbiased analysis of immunophenotyping of blood from a cohort of moderate, severe, recovered COVID-19 donors and healthy control reveals selective clustering of severe individuals. Severe cases have oligoclonal expansion of antibodies, which have long CDR3s, indicating multi-reactivity, compared to moderate cases that had a more diverse clonal expansion. The increased frequencies of neutrophil and eosinophil populations were not associated with an activated phenotype. They highlight a decrease of CD15 expression in neutrophils and CD16 expression in different innate immune cell populations such as NK cells that could be interesting to investigate further. Standing in the field: They show extensive data on blood immune cell composition between different subgroups of COVID-19 patients that supports other studies, which they always cite. Appropriate statistics: The statistical tools used are very clear and all listed in the method section of the paper. Viral model used: SARS-CoV2 from ...
Cisco-Eagle Catalog - 40 Series Swivel Caster with 4" x 1-1/2" Urethane on Plastic Wheel and 500 lb. Capacity
Urethane on Poly wheel utilizes polyurethane mechanically bonded to a high-density polypropylene core. This mechanical bond eliminates bond separation due to
Characterization of Vitreous B-Cell Infiltrates in Patients with Primary Ocular Lymphoma, Using CDR3 Size Polymorphism Analysis...
For CDR3 size polymorphism analysis we used the Immunoscope software package. 23 Immunoscope analysis couples fluorescence PCR and software analysis and allows the direct and accurate sizing of a clonal population of T or B cells within a polyclonal milieu. Peripheral blood mononuclear cells (PBMCs) were prepared by centrifugation on single-density gradient (Ficoll Hypaque; Pharmacia, Upsala, Sweden) and processed for Immunoscope analysis, as previously described. 25 An Epstein-Barr virus (EBV)-transformed human B lymphoblastoid cell line (BLCL) was established in the laboratory and maintained as previously described. 26 The vitreous humor was obtained after vitrectomy. Approximately 200 μL of vitreous humor was diluted in a saline solution to a final volume of 1 mL, and the samples were centrifuged immediately (910g for 10 minutes at 4°C). Previous morphologic studies revealed that such amounts of vitreous humor obtained from PIOL patients contain, on average, 1000 cells (Merle-Beral H, ...
Frontiers | Repertoire analysis of antibody CDR-H3 loops suggests affinity maturation does not typically result in...
Antibodies can rapidly evolve in specific response to antigens. Affinity maturation drives this evolution through cycles of mutation and selection leading to enhanced antibody specificity and affinity. Elucidating the biophysical mechanisms that underlie affinity maturation is fundamental to understanding B-cell immunity. An emergent hypothesis is that affinity maturation reduces the conformational flexibility of the antibodys antigen-binding paratope to minimize entropic losses incurred upon binding. In recent years, computational and experimental approaches have tested this hypothesis on a small number of antibodies, often observing a decrease in the flexibility of the Complementarity Determining Region (CDR) loops that typically comprise the paratope and in particular the CDR-H3 loop, which contributes a plurality of antigen contacts. However, there were a few exceptions, and previous studies were limited to a small handful of cases. Here, we determined the structural flexibility of the CDR-H3 loop
Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile | BMC Biology | Full Text
CD4 T lymphocyte activation requires T cell receptor (TCR) engagement by peptide/MHC (major histocompatibility complex) (pMHC). The TCR complementarity-determining region 3 (CDR3) contains variable α and β loops critical for pMHC recognition. During any immune response, tuning of TCR usage through progressive clonal selection occurs. Th1 and Th2 cells operate at different avidities for activation and display distinct transcriptional programs, although polarization may be plastic, influenced by pathogens and cytokines. We therefore hypothesized that CDR3αβ sequence features may intrinsically influence CD4 phenotype during progression of a response. We show that CD4 polarization involves distinct CDR3α usage: Th1 and Th17 cells favored short TCR CDR3α sequences of 12 and 11 amino acids, respectively, while Th2 cells favored elongated CDR3α loops of 14 amino acids, with lower predicted affinity. The dominant Th2- and Th1-derived TCRα sequences with14 amino acid CDR3 loops and 12 amino acid CDR3
Arthur M. Lesk - Wikipedia
Lesk has made significant contributions to the study of protein evolution. He and Cyrus Chothia, working at the Medical Research Council (UK) Laboratory of Molecular Biology in Cambridge, United Kingdom, discovered the relationship between changes in amino-acid sequence and changes in protein structure by analyzing the mechanism of evolution in protein families. This discovery has provided the quantitative basis for the most successful and widely used method of structure prediction, known as homology modelling. Lesk and Chothia also studied the conformations of antigen-binding sites of immunoglobulins. They discovered the canonical-structure model for the conformation of the complementarity-determining regions of antibodies, and they applied this model to the analysis of antibody-germ-line genes, including the prediction of the structure of the corresponding proteins. This work has supported the humanization of antibodies for therapy in the treatment of cancer. This approach to ...
Splicing in Diversity | Science
The antigen-binding end of an antibody molecule and green fluorescent protein (GEP) both contain β-strands that are connected by exposed loops. Zeytun et al. have taken advantage of this commonality and engineered libraries of fluorobodies by inserting antigen-binding sequences (the complementarity-determining regions) within four of the GFP loops. They isolated fluorobodies that bound specifically to several proteins (ubiquitin, tubulin, and myoglobin) with submicromolar affinities and were used successfully in immunofluorescence microscopy, immunoblots, or flow cytometry. - GJC. NatureBiotechnol. 10.1038/nbt911 (2003).. ...
Surface representation of the CDRs.(A) Surface represen | Open-i
Surface representation of the CDRs.(A) Surface representation of D7 revealing the gp120 docking interface based on gp120 binding and HIV-1 neutralization result
Ebook 978-1441948472 Complementarity: Applications, Algorithms and Extensions (Applied Optimizati
Please Note, The ebooks are not always PDF format, you might receive epub/kindle formats after purchase. This is Digital Version of (Ebook) 978-1
YUHSpace: T 세포 백혈병 세포주에서 T 세포 수용체 β쇄 CDR3 유전자 염기서열의 규명
title: T 세포 백혈병 세포주에서 T 세포 수용체 β쇄 CDR3 유전자 염기서열의 규명, doi: none, category: Thesis
Circulating T cell repertoire complexity in normal individuals and bone marrow recipients analyzed by CDR3 size spectratyping....
The analysis of the T cell repertoires involved in local or systemic immune responses is beginning to play an important role in many clinical situations. These include autoimmunity, response to viral or bacterial superantigens, alloimmunity including allograft rejection, and tumor immunity. Here we analyze circulating T cell repertoires by determining TCR beta-chain gene complexity using a modification of V beta family-specific PCR. This approach, called CDR3 size spectratyping, uses the size heterogeneity of the CDR3 as a further source of specificity in TCR analysis. It has been used here to analyze the complexity and stability of circulating T cell repertoires in normal adults, including bone marrow donors, and bone marrow transplant recipients. Normal spectratypes are both complex and stable. The repertoire complexity of marrow recipients correlates with their state of immune function. Contractions and gaps in repertoires are revealed in individuals suffering from recurrent infections ...
Age, microbiota, and T cells shape diverse individual IgA repertoires in the intestine | JEM
The intestinal mucosa is constantly exposed to microbial and dietary antigens, all of which are considered to induce IgA-secreting plasma cells. Thus, the limited IgA repertoire diversity predicted in previous studies (Dunn-Walters et al., 1997, 2000; Holtmeier et al., 2000; Stoel et al., 2005; Yuvaraj et al., 2009) is difficult to reconcile with the broad range of intestinal antigens. In this study, we show that in fact each individual harbors a private polyclonal and highly diverse IgA repertoire. Because we analyzed switched Ig sequences, repertoire analyses performed in this study did not require sorting of plasma cells. Instead, RNA was isolated directly from intestinal tissue, and IgA-encoding transcripts were enriched during reverse transcription. This approach was equivalent to our results obtained when RNA was isolated out of sorted plasma cells. The IgA repertoire showed characteristics reminiscent of the T cell receptor repertoire of CD8+ memory cells (Naumov et al., 2003), i.e., the ...
Mouse TCR Profiling Kit
The SMARTer Mouse TCR a/b Profiling Kit provides a powerful new solution for those seeking to perform T-cell receptor repertoire analysis using NGS.
Mouse TCR Profiling Kit
The SMARTer Mouse TCR a/b Profiling Kit provides a powerful new solution for those seeking to perform T-cell receptor repertoire analysis using NGS.
Opinion: Predicting the Efficacy of Cancer Immunotherapy | The Scientist Magazine®
T-cell receptor repertoires could help researchers determine whether a certain treatment will work for a given cancer patient.
Supraorbital Nerve Block | Migraine Treatment | Chicago
If youre suffering from a headache or migraine in the forehead that doesnt go away, a supraorbital nerve block may help. Call for a free consultation.
Frontiers | High-throughput sequencing of the expressed torafugu (Takifugu rubripes) antibody sequences distinguishes IgM and...
B cell antigen receptor (BCR) or antibody diversity arises from somatic recombination of immunoglobulin (Ig) gene segments and is concentrated within the Ig heavy (H) chain complementarity-determining region 3 (CDR-H3). We performed high-throughput sequencing of the expressed antibody heavy chain repertoire from adult torafugu. We found that torafugu use between 70% and 82% of all possible V (variable) D (diversity) J (joining) gene segment combinations and that they share a similar frequency distribution of these VDJ combinations. The CDR-H3 sequence repertoire observed in individuals is biased with the preferential use of a small number of VDJ, dominated by sequences containing inserted nucleotides. We uncovered the common CDR-H3 amino acid (aa) sequences shared by individuals. Common CDR-H3 sequences feature highly convergent nucleic acid recombination compared with private ones. Finally, we observed differences in repertoires between IgM and IgT, including the unequal usage frequencies of V gene
Longitudinal analysis of peripheral blood T cell receptor diversity in patients with systemic lupus erythematosus by next...
T cells play an important role in the pathogenesis of systemic lupus erythematosus (SLE). Clonal expansion of T cells correlating with disease activity has been observed in peripheral blood (PB) of SLE subjects. Recently, next-generation sequencing (NGS) of the T cell receptor (TCR) β loci has emerged as a sensitive way to measure the T cell repertoire. In this study, we utilized NGS to assess whether changes in T cell repertoire diversity in PB of SLE patients correlate with or predict changes in disease activity. Total RNA was isolated from the PB of 11 SLE patients. Each subject had three samples, collected at periods of clinical quiescence and at a flare. Twelve age-matched healthy controls (HC) were used for reference. NGS was used to profile the complementarity-determining region 3 (CDR3) of the rearranged TCR β loci. Relative to the HC, SLE patients (at quiescence) demonstrated a 2.2-fold reduction in repertoire diversity in a given PB volume (P |0.0002), a more uneven distribution of the
Expanded CD4+ and CD8+ T cell clones in elderly humans. | The Journal of Immunology
The diversity of the human TCR repertoire in aging has been studied by examining the profiles of complementarity-determining region 3 (CDR3) sizes expressed by the BV families. The TCRBV CDR3 profile, which shows size heterogeneity in young adult humans, is significantly restricted in aged humans. Clonal T cell expansions were identified using a PCR-based approach, in one or more BV families from all 14 healthy persons over the age of 65 that we studied. CD4+ T cell expansions were identified in 8 of 11 donors and CD8+ T cell expansions in 7 of 10 donors. These clonal expansions were stable during a 2-year period. Interestingly, more than half of the aged persons had clonal expansions within the BV3, -14, -16, and -23 families. Although there was no homology among the eight CDR3 sequences identified in clonal T cells from 8 aged persons, selective pressure on the expanded T cell clones was suggested by the fact that the BV families used by the T cell clones were not proportional to the number of ...
In Search of Complementarity in Innovation Strategy: Internal R& D and External Knowledge Acquisition
Downloadable! Empirical research on complementarity between organizational design decisions has traditionally focused on the question of existence of complementarity. In this paper, we take a broader approach to the issue, combining a productivity and an adoption approach, while including a search for contextual variables in the firms strategy that affects complementarity. Analysis of contextual variables is not only interesting per se, but also improves the productivity test for the existence of complementarity. We use our empirical methodology to analyze complementarity between innovation activities: internal research and development (R& D) and external knowledge acquisition. Our results suggest that internal R& D and external knowledge acquisition are complementary innovation activities, but that the degree of complementarity is sensitive to other elements of the firms strategic environment. We identify reliance on basic R& D--the importance of universities and research centers as an
Staff - Institute of Genetics and Biophysics
1. The regulation of the immune response: vaccine design, phage-based vaccines, Virus-like-particle vaccines, protein vaccines, Alzheimers Disease immunotherapy, induction of immune responses to self-antigens, correlates of vaccine efficacy, signaling in T cell development and activation, T cell receptor repertoire in Coeliac disease, T cell receptor repertoire of antigen-specific T cell lines ...
Re: display-buffer-alist simplifications
functions (append (car display-buffer-overriding-action) , (car user-action) , (car action) , (car display-buffer-default-action))) , (alist (append (cdr display-buffer-overriding-action) , (cdr user-action) , (cdr action) , (cdr display-buffer-default-action)))) , (run-with-args-until-success functions buffer alist))) Elaborating once more on this subject: Let O, U, A and D respectively denote the functions in the cars of display-buffer-overriding-action, user-action, action, and display-buffer-default-action and OL, UL, AL, DL the alists in the corresponding cdrs. Now my original proposal was to run until success (O . (OL UL AL DL)) (U . (UL AL DL)) (A . (AL DL)) (D . (DL)) that is, try the overriding function with all alists, if that fails try the user function with all alists but the overriding one and so on. People raised concerns about misinterpreting the alist concept and Chong proposed to do something like (O . (OL)) (U . (UL)) (A . (AL)) (D . (DL)) instead. I raised concerns about ...
Clinical Data Review (CDR) | PerkinElmer
Clinical data review solution to quickly anticipate and act on clinical/medical review questions, uncover trends, and risks through self-service discovery.
Test alphabetical | Viapath
Use the search fields below to explore our most up to date test repertoire and find user details such as how to order and interpret results. ...
21 tunes in 21 weeks......Week #17(all contributors) | Page 11 | The Accordionists Forum
1. Its possible and instructive to build repertoire by adding one tune a week and concentrating my practicing and playing to these tunes. Yes, indeed. I...
The T cell receptor repertoire in synovial tissue of patients with early rheumatoid arthritis (RA) is dominated by highly...
Material and methods Three DMARD-naïve ERA patients (disease duration , 1 year) and three ESRA patients (disease duration ,2 years) were included. All fulfilled the American College of Rheumatology1987 criteria for RA. mRNA was isolated from paired ST and PB samples. A linear amplification with primers for all V(ariable)-genes of the T cell receptor β-chain was performed. The amplified products contain the CDR3s of all T cells. Samples were processed using a Genome Sequencer (454) analysing up to 15 000 receptors per sample, The frequency of each clone was determined by its CDR3 frequency (% of all CDR3s analysed). Clones with a CDR3 frequency of ,1% were arbitrarily considered as highly expanded clones (HECs).. ...
In this report, we describe the molecular and functional characterization of T-cell clones identified in peripheral blood from patients with relapsed myeloma responding to DLI. These clones were initially identified through analysis of TCR Vβ repertoire (spectratyping), a technique that provides a comprehensive characterization of the circulating T-cell compartment (21 , 24 , 25) . Serial analysis of T-cell repertoire in patient samples has also been used to detect the emergence of oligoclonal and clonal T cells at different times in vivo (18 , 26 , 27) . By combining analysis of TCR repertoire with clinical events, we observed the expansion of individual T-cell clones in peripheral blood that were temporally associated with the initiation of either a GVM or GVHD response (17) . However, despite the association of these T-cell clones with specific clinical responses, the functional specificity of these T cells was not established. Further studies were therefore undertaken to quantify the ...
Malawi CDR Spy Machine Coming Soon | Commsrisk
There is a reason this project is unique. It is because governments and regulators in other countries see no reason to collect every single CDR for every single call. If others do not have a system like this, how can Malawis authorities make extraordinary promises about improving services as well as gathering more taxes?. Awareness of the perils of CDR collection has moved on since MACRA initiated their project, even if MACRAs arguments have not changed at all. The world can turn its eyes toward the example set by the USA, whose authorities also needed to justify blanket collection of CDRs. In that case, the leak of secret court orders revealed that the NSA was gathering huge swathes of call data. The subsequent justification offered by the NSA was that the data is needed to identify relationships between potential terrorists. In other words, the NSA gathers CDRs for the purpose of surveillance. Meanwhile, Malawis government says they will implement a comprehensive centralized database of ...
DI-fusion A quantitative theory of affinity-driven T cell repertoire...
DI-fusion, le Dépôt institutionnel numérique de lULB, est loutil de référencementde la production scientifique de lULB.Linterface de recherche DI-fusion permet de consulter les publications des chercheurs de lULB et les thèses qui y ont été défendues.
India, by pivoting to Quad, will ruin its industrial chain - Global Times
China, as expected, has remained the largest source of Indias imports in 2020, highlighting the economic complementarities of the two Asian economies which is exactly what could help India to promote its
REWIRING THE SOUL with Gabriella Kortsch, Ph.D.: Tips For Creating a Different Future
It is precisely at the problematic crossroads so often encountered in relationships that we are offered the opportunity to create a new foundation based on mutual complementarity rather than need; a free relationship between two people who want to be together, rather than two people who need to be together. Needing another, we are told, is the measure of love, but for a fully conscious individual nothing could be further from the truth. And therein lies part of the secret and healing power of spiritual partnerships. ...
CRIS.FAU.DE - Current Research Information System of FAU
Human cytomegalovirus (HCMV) infections are life-threating to people with a compromised or immature immune system. Upon adhesion, fusion of the virus envelope with the host cell is initiated. In this step, the viral glycoprotein gB is considered to represent the major fusogen. Here, we present for the first time structural data on the binding of an anti-herpes virus antibody and describe the atomic interactions between the antigenic domain Dom-II of HCMV gB and the Fab fragment of the human antibody SM5-1. The crystal structure shows that SM5-1 binds Dom-II almost exclusively via only two CDRs, namely light chain CDR L1 and a 22-residue-long heavy chain CDR H3. Two contiguous segments of Dom-II are targeted by SM5-1, and the combining site includes a hydrophobic pocket on the Dom-II surface that is only partially filled by CDR H3 residues. SM5-1 belongs to a series of sequence-homologous anti-HCMV gB monoclonal antibodies that were isolated from the same donor at a single time point and that ...
DETECTION AND QUANTIFICATION OF SAMPLE CONTAMINATION IN IMMUNE REPERTOIRE ANALYSIS - Patent application
0094] Clonotype profile means a listing of distinct clonotypes and their relative abundances that are derived from a population of lymphocytes. Typically, the population of lymphocytes are obtained from a tissue sample. The term clonotype profile is related to, but more general than, the immunology concept of immune repertoire as described in references, such as the following: Arstila et al. Science, 286: 958-61 (1999): Yassai et al. Imnunogenetics, 61: 493-502 (2009); Kedzierska et al, Mol. Immunol., 45(3): 607-618 (2008); and the like. The term clonotype profile includes a wide variety of lists and abundances of rearranged immune receptor-encoding nucleic acids, which may be derived from selected subsets of lymphocytes (e.g. tissue-infiltrating lymphocytes, immunophenotypic subsets, or the like), or which may encode portions of immune receptors that have reduced diversity as compared to full immune receptors. In some embodiments, clonotype profiles may comprise at least 103 distinct ...
Aalysis of immune receptor diversity and oligoclonality in responses to vaccinati - Andrew Fire
We will carry out deep sequencing of rearranged immunoglobulin (Ig) and T cell receptor (TCR) genes from lymphocytes in human subjects responding to several dis...
Aalysis of immune receptor diversity and oligoclonality in responses to vaccinati - Andrew Fire
We will carry out deep sequencing of rearranged immunoglobulin (Ig) and T cell receptor (TCR) genes from lymphocytes in human subjects responding to several dis...
The Curious Wavefunction: On the primacy of doubt in an age of illusory certainty
Its this seemingly paradoxical and yet crucial yin-and-yang aspect of science that I believe is still quite hard to grasp for non-scientists. Niels Bohr would have appreciated the tension. Bohr bequeathed to the world the concept of complementarity. Complementarity means the existence of seemingly opposite ideas that are still required together to explain the world. In the physical world, complementarity was first glimpsed in the behavior of subatomic particles which can sometimes behave as waves and sometime as particles, depending on the experiment. Waves and particles may seem to be contradictory concepts, and yet as the pioneers of quantum mechanics showed us, you cannot explain reality without assuming that electrons or photons are both. In his later life, Bohr extended the idea of complementarity to many aspects of the human world; good and evil, freedom and restraint, war and peace. He realized that all of these seemingly paradoxical aspects of the human and physical world have to ...
pI profiles across HC CDR3 regions of NZM2410-derived A | Open-i
pI profiles across HC CDR3 regions of NZM2410-derived ANAs and non-ANAs. The mean pI value (isoelectric point) at each HC CDR3 position (from H95 to H100a) was
Détermination des polyphénols totaux dans le vin et le moût avec CDR WineLab
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Gentaur Molecular :GeneRiver \ Spleen cDNA \ CDR007
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Gentaur Molecular :GeneRiver \ Stomach cDNA \ CDR006
Gentaur molecular products has all kinds of products like :search , GeneRiver \ Stomach cDNA \ CDR006 for more molecular products just contact us
Use 80 min. / 730 MB Cdr where nessecary !!. I can copy all of my recordings to CDr. If Its from LP or tape/video you can be sure its remastered before I make a Cdr of it. So NO crackles from boot LPs on my Cdrs!!!! ...
02-2016-Factors Affecting Anti-Glycan IgG and IgM Repertoires in Human Serum
Хранение и публикация учебных и учебно-тематических материалов, все для учебы
화학공학소재연구정보센터(CHERIC) | 연구정보 | 문헌DB | 학술지 검색
Immunoglobulins play important roles in antigen recognition during the immune response, and the complementarity-determining region (CDR) 3 of the heavy chain is considered as the critical antigen-binding site. We previously developed a statistical protocol for the extensive analysis of heavy chain variable region repertoires and the dynamics of their immune response using next-generation sequencing (NGS). The properties of important antibody heavy chains predicted in silico by the protocol were examined by gene synthesis and antibody protein expression; however, the corresponding light chain that matches with the heavy chain could not be predicted by our protocol. To understand the dynamics of the heavy chain and the effect of light chain pairing on it, we firstly tried to obtain an artificial light chain that pairs with a broad range of heavy chains and then analyzed its effect on the antigen binding of heavy chains upon pairing. During the pre-B cell stage, the surrogate light chain (SLC) ...
indieville: review: wayne butane - backwash
Backwash is a ten-year compilation of Wayne Butanes talk-heavy plunderphonics collages. It collects seven cassette recordings and one seven-inch record into one long CDR track. The work is somewhat akin to the less musical side of People Like Us and The Tape Beatles, with emphasis on vocal clips rather than song excerpts - which are also present, but sparse. Like Swipes, which I also reviewed recently, this disc is successful because of its sample diversity - theres so much on here, that you just end up sitting next to the stereo with your mouth gaping open in awe. Sound sources come from everywhere - grisly newscasts, Michael Jackson, Longmont Potion Castle, and even pornography. This isnt nearly as polished as some of the more popular audio collage material, but its a good selection of lo-fi cut-n-pasting. Fun.. 85%. Matt Shimmer ...
We have humanized a murine anti-GM2 MAb in an attempt to improve its potential clinical efficacy by reducing its immunogenicity and by changing the C region to support potent CDC and ADCC. The pharmacokinetic studies of humanized MAbs and murine MAbs in monkeys revealed that the humanization resulted in a prolonged serum half-life and in a substantial reduction in immunogenicity compared with the murine MAbs (44 , 45) . The antihumanized KM8969 response would be theoretically directed to a conformational epitope formed by the CDR loops, so that the reduction in immune response to the KM8969 in monkeys and humans would be expected in comparison with the chimeric KM966, which has murine V regions including CDRs. There has been no comparative pharmacokinetic studies between the humanized MAbs and their counterpart chimeric MAbs. The actual advantages of the humanized MAbs in pharmacokinetics vary for each MAb and need to be evaluated in clinical studies.. The humanized KM8969 showed a binding ...
Repertoire Bibliographique Des Principales Revues Francaises, : Daniel Jordell : 9781236769947
Repertoire Bibliographique Des Principales Revues Francaises, by Daniel Jordell, 9781236769947, available at Book Depository with free delivery worldwide.
These regions correspond to the complementarity-determining regions; the sites involved in antigen recognition on the ... The TSM process implies an "in-frame DNA reader" whereby DNA and RNA deaminases at transcribed regions are guided in their ... During B cell division the immunoglobulin variable region DNA is transcribed and translated. The introduction of mutations in ... Somatic hypermutation involves a programmed process of mutation affecting the variable regions of immunoglobulin genes. Unlike ...
... each have distinctly formed complementarity-determining regions. Allergen - A substance capable of causing an allergic reaction ...
These loops are referred to as the complementarity-determining regions (CDRs), since their shape complements that of an antigen ... or complementarity-determining regions (CDR1, CDR2 and CDR3). CDRs are supported within the variable domains by conserved ... multi-level conformational clustering of antibody complementarity-determining regions". PeerJ. 2 (e456): e456. doi:10.7717/ ... The distinctive features of each class are determined by the part of the heavy chain within the hinge and Fc region. The ...
Humanized antibodies are almost completely human; only the complementarity determining regions of the variable regions are ... Changes in the Fc region can alter an antibody's ability to engage Fc receptors and, by extension, will determine the type of ... Anti-PD-1 drugs contain not only an Fab region that binds PD-1 but also an Fc region. Experimental work indicates that the Fc ... Antibodies are formed of a binding region (Fab) and the Fc region that can be detected by immune system cells via their Fc ...
1986 in science
"Replacing the complementarity-determining regions in a human antibody with those from a mouse". Nature. 321 (6069): 522-525. ...
"Humanization of rabbit monoclonal antibodies via grafting combined Kabat/IMGT/Paratome complementarity-determining regions: ... The region (residues 296-359) consisting of 64 amino acids at the N-terminus of cell surface mesothelin has been identified as ... depending on the assay used and thus that normal levels must be determined anew when new assays are introduced. Increase of ... including a poorly immunogenic region (the YP218 site) at the C terminus of human mesothelin. The rabbit antibodies have been ...
They named "HV4" and "LV4" in rabbit Fvs, non-complementarity-determining region (CDR) loops that are structurally close to the ... "Humanization of rabbit monoclonal antibodies via grafting combined Kabat/IMGT/Paratome complementarity-determining regions: ...
... despite the non-human origin of some of its complementarity-determining region (CDR) segments responsible for the ability of ... "Humanization of an anti-CD34 monoclonal antibody by complementarity-determining region grafting based on computer-assisted ... mouse variable region spliced to human constant region). Thereafter the chimera might be further humanized by the selective ... That is, since the CDR portions of the variable region are essential to the ability of the antibody to bind to its intended ...
However, they have demonstrated that two of the five consensus mutations were within the complementarity determining regions ( ... The T7 promoter region allows large-scale in vitro T7 transcription to transcribe the DNA library into an mRNA library, which ... The ribosomal binding site in the 5'-untranslated region (5' UTR) is designed according to the in vitro translation system to ...
... between Antibody 2F5 Neutralization of HIV-1 and Hydrophobicity of Its Heavy Chain Third Complementarity-Determining Region". ... The region DKW of the core epitope must be in a β-turn conformation and have the correct side-chain positions for 2F5 to bind ... 2F5 recognizes an epitope in the membrane-proximal external region (MPER) of HIV-1 gp41. 2F5 then binds to this epitope and its ... 2F5 binds to the variable regions of env and neutralizes the virus before it infects target cells. 2F5 recognizes a core ...
Each paratope is made up of six complementarity-determining regions - three from each of the light and heavy chains - that ... In cows, an extra-long complementarity-determining region is considered to have an essential role in diversifying paratopes. ... It is a small region at the tip of the antibody's antigen-binding fragment and contains parts of the antibody's heavy and light ...
Polymeric immunoglobulin receptor
Evidence for the involvement of multiple complementarity determining region (CDR)-like loops in receptor domain I". The Journal ... 5'-flanking region of the Pigr gene contains a response element to glucocorticoids. This class of hormones increases the steady ... Cleavage occurs at the junction of the transmembrane region of the receptor and domain 5. pIgRs are capable of capturing IgA ... The quite long intracellular domain of the receptor, alongside with the transmembrane region, is responsible for the ...
The variable domain of both the TCR α-chain and β-chain each have three hypervariable or complementarity-determining regions ( ... The Constant region is proximal to the cell membrane, followed by a transmembrane region and a short cytoplasmic tail, while ... Each chain is composed of two extracellular domains: Variable (V) region and a Constant (C) region, both of Immunoglobulin ... The intersection of these specific regions (V and J for the alpha or gamma chain; V, D, and J for the beta or delta chain) ...
The variable domain of both the TCR α-chain and β-chain each have three hypervariable or complementarity determining regions ( ... The Constant region is proximal to the cell membrane, followed by a transmembrane region and a short cytoplasmic tail, while ... Each chain is composed of two extracellular domains: Variable (V) region and a Constant (C) region, both of Immunoglobulin ... The residues in these variable domains are located in two regions of the TCR, at the interface of the α- and β-chains and in ...
The variable region of antigen receptors of T cells (TCRs) and B cells (immunoglobulins) contain complementarity-determining ... They define the surface and properties of the variable region, determining the antigen specificity and therefore the idiotope ... He also defined the "paratope" to be that part of an antibody variable region that binds to an antigen. The best developed ... Antibody idiotype is determined by: Gene rearrangement Junctional diversity P-nucleotides (palindromic nucleotides at sites of ...
The complementarity-determining regions of 5.11A1 were cloned into the framework of human IgG and combined with IgG1 (TGN1112) ... The molecule was genetically engineered by transfer of the complementarity determining regions (CDRs) from heavy and light ... Humanised variable regions were subsequently recombined with a human gene coding for the IgG4 gamma chain and with a human gene ... region. According to a report by TeGenero, the F(ab)2 is not able to generate the required stimulation. Unlike the related ...
The variable domain contains the paratope (the antigen-binding site), comprising a set of complementarity-determining regions, ... The antigen-binding fragment (Fab) is a region on an antibody that binds to antigens. It is composed of one constant and one ... Conversely, the enzyme pepsin cleaves below the hinge region, so the result instead is a F(ab')2 fragment and a pFc' fragment. ... Heavy and light chains, variable and constant regions of an antibody. An antibody digested by papain yields three fragments: ...
One end of the Ig domain has a section called the complementarity-determining region that is important for the specificity of ... It is believed that the structure of variable subgenes of Ig and the surface immunoglobulin determine the propensity of chronic ...
Nest (protein structural motif)
... complementarity determining regions) bound to a carboxylate side chain. These have been engineered to give rise to monoclonal ... Their occurrence in cation and anion-binding regions of proteins". Journal of Molecular Biology. 315 (15): 183-191. doi:10.1006 ... successive residues gives rise to anion-binding sites that occur commonly and are found often at functionally important regions ...
It is an immunoglobulin G-kappa (IgGκ) consisting of human constant regions and murine complementarity-determining regions ... per person per year price and Pharmac's economic analysis determined the price would need to be halved before the drug was cost ... grafted onto human framework light and heavy chain variable regions. The compound contains two 448-amino acid heavy chains and ...
The antibody, originally called G5/44, was created by grafting the complementarity-determining regions and some framework ...
... now known as complementarity-determining region 3. Her research also pointed to evidence that at least two genes are involved ... Her studies on antibodies were important in determining the chain structure, and particularly the observation that more than ... Press's work provided the first evidence that immunoglobulin heavy chains had variable regions similar to those observed in ...
... can refer to Complementarity-determining region 2 on antibodies CDR2 (gene), cerebellar degeneration-related protein 2, a ...
Drug reaction with eosinophilia and systemic symptoms
... one study identified the preferential presence of the TCR-V-b and complementarity-determining region 3 in T-cell receptors ... studies to date have not clearly determined if they are a cause or merely a consequence of T cell-mediated tissue injury. ...
... today called the complementarity-determining regions (CDRs). Affinity therapy, or immunotoxins is a biorecognition-based ...
Arthur M. Lesk
They discovered the "canonical-structure model" for the conformation of the complementarity-determining regions of antibodies, ... each peptide plane is determined for Ribbon Diagrams and β-sheets; and spline fit is used for curved sheets. Hidden-line ... "Conformations of immunoglobulin hypervariable regions". Nature. 342 (6252): 877-883. Bibcode:1989Natur.342..877C. doi:10.1038/ ...
List of MeSH codes (D12.776)
... immunoglobulin variable region MeSH D12.776.377.715.548.680.650.500.180 - complementarity determining regions MeSH D12.776. ... immunoglobulin variable region MeSH D12.776.377.715.548.797.180 - complementarity determining regions MeSH D12.776.377.715. ... complementarity determining regions MeSH D12.776.377.715.548.114.525 - hemolysins MeSH D12.776.377.715.548.114.573 - immune ... sex-determining region y protein MeSH D12.776.664.235.400.800 - tcf transcription factors MeSH D12.776.664.235.400.800.500 - ...
List of MeSH codes (D12.776.124)
... immunoglobulin variable region MeSH D12.776.124.486.485.680.650.500.180 - complementarity determining regions MeSH D12.776. ... immunoglobulin variable region MeSH D12.776.124.486.485.797.180 - complementarity determining regions MeSH D12.776.124.486. ... immunoglobulin variable region MeSH D12.776.124.790.651.680.650.500.180 - complementarity determining regions MeSH D12.776. ... complementarity determining regions MeSH D12.776.124.486.485.114.525 - hemolysins MeSH D12.776.124.486.485.114.573 - immune ...
... one study identified the preferential presence of the TCR-V-b and complementarity-determining region 3 in T-cell receptors ... Determining what drug is the cause is based on the time interval between first use of the drug and the beginning of the skin ... but also in the genital and anal regions. Those in the mouth are usually extremely painful and reduce the patient's ability to ... or TEN and the intake of specific drugs as determined in certain populations are given in HLA associations with SCARs. In ...
Fluorescence in situ hybridization
Bacterial FISH probes are often primers for the 16s rRNA region. FISH is widely used in the field of microbial ecology, to ... The mixture of probe sequences determines the type of feature the probe can detect. Probes that hybridize along an entire ... uses fluorescent probes that bind to only those parts of a nucleic acid sequence with a high degree of sequence complementarity ... However, it is possible to create a mixture of smaller probes that are specific to a particular region (locus) of DNA; these ...
The chosen insect vector of a plant virus will often be the determining factor in that virus's host range: it can only infect ... 2001). "In vivo analysis of the TSWV cap-snatching mechanism: single base complementarity and primer length requirements". The ... untranslated regions of viral mRNA. Some viruses (e.g. tobacco mosaic virus (TMV)) have RNA sequences that contain a "leaky" ... although he did not determine that the RNA was the infectious material. However, he received the Nobel Prize in Chemistry in ...
It can also be shown that the singular region contains all the mass of the black hole solution. The singular region can ... If there are other stars orbiting a black hole, their orbits can be used to determine the black hole's mass and location. Such ... Black hole complementarity. *Black Hole Initiative. *Black holes in fiction. *Black hole starship ... A black hole is a region of spacetime exhibiting such strong gravitational effects that nothing-not even particles and ...
The RBS in prokaryotes is a region upstream of the start codon. This region of the mRNA has the consensus 5'-AGGAGG-3', also ... Richer complementarity results in higher initiation efficiency. It is worth noting that this only holds up to a certain ... The identification of RBSs is used to determine the site of translation initiation in an unannotated sequence. This is referred ... The level of complementarity of the mRNA SD sequence to the ribosomal ASD greatly affects the efficiency of translation ...
When these magnetic patterns were mapped over a wide region, the ocean floor showed a zebra-like pattern: one stripe with ... Where the plates meet, their relative motion determines the type of boundary: convergent, divergent, or transform. Earthquakes ... Since that time many theories were proposed to explain this apparent complementarity, but the assumption of a solid Earth made ... Reconstruction is used to establish past (and future) plate configurations, helping determine the shape and make-up of ancient ...
Introduction to quantum mechanics
Wave-particle duality is an example of the principle of complementarity in quantum physics. An elegant ... A field in physics is "a region or space in which a given effect (such as magnetism) exists." Other effects that manifest ... So the two photons come out purple. If the experimenter now performs some experiment that determines whether one of the photons ... This is a typical feature of quantum complementarity: a quantum particle acts as a wave in an experiment to measure its wave- ...
Black hole thermodynamics
From the first law of black-hole mechanics, this determines the multiplicative constant of the Bekenstein-Hawking entropy, ... This area relationship was generalized to arbitrary regions via the Ryu-Takayanagi formula, which relates the entanglement ... Black hole complementarity. *Black hole information paradox. *Black-hole thermodynamics. *Bousso's holographic bound ...
See also: Complementarity-determining region and Framework region. See also. *Cambridge Reference Sequence ... A highly polymorphic nuclear or mitochondrial DNA region. A hypervariable region (HVR) is a location within nuclear DNA or the ... HVR1 is considered a "low resolution" region and HVR2 is considered a "high resolution" region. Getting HVR1 and HVR2 DNA tests ... Human mitochondrial genome showing hypervariable regions I to III (green boxes) located in the control region (CR; grey box). ...
International trade theory
Wages determined in this model are different according to the productivity of countries. The model is therefore more suitable ... Golub, S. S. (1995) Comparative and absolute advantage in the Asia-Pacific region (No. 95-09). Federal Reserve Bank of San ... Matsuyama, K. (2000). "A Ricardian Model with a Continuum of Goods under Nonhomothetic Preferences: Demand Complementarities, ... The results of the H-O model are that the pattern of international trade is determined by differences in factor endowments. It ...
Interpersonal complementarityEdit. The interpersonal complementarity hypothesis suggests that obedience and authority are ... the status of a male Canada goose is determined by the rank of his family. Although dominance is determined differently in each ... a region of the female wasp brain responsible for the synthesis and secretion of JH, are naturally more dominant. In an ... Determining the outcome of conflictEdit. Patterns of animal conflict reveal important insights into the evolution of behavior ...
Benzer S. FINE STRUCTURE OF A GENETIC REGION IN BACTERIOPHAGE. Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):344-54. PMID ... that the amino acid sequence of a protein is specified by the nucleotide sequence of the gene determining the protein. Thus, ... and his belief that a complementarity principle, perhaps like that needed for understanding quantum mechanics, would be the key ... Benzer S. "Adventures in the rII region" in Phage and the Origins of Molecular Biology (2007) Edited by John Cairns, Gunther S ...
De sünnere Verännerlichkeit vun de Antigenbinnensteed (Complementarity Determining Region, kort: CDR) maakt de Organismus över ... hinge region) liggen andelen vun de sworen Keden dat Fragment, dat an de Antigenen anbinnt (Fab) un enzymaatsch. mit Help vun ...
Long non-coding RNA
Sequence complementarity between BC1 and regions of various neuron-specific mRNAs also suggest a role for BC1 in targeted ... However, it was not determined if the protein products were required for pluripotency. Peptides encoded by human lncRNAs have ... Morey C, Navarro P, Debrand E, Avner P, Rougeulle C, Clerc P (February 2004). "The region 3' to Xist mediates X chromosome ... September 2007). "Ultraconserved regions encoding ncRNAs are altered in human leukemias and carcinomas". Cancer Cell. 12 (3): ...
The infinite potential walls of the box determine the values of C, D, and k at x = 0 and x = L where ψ must be zero. Thus, at x ... Bohr, N. (1948). On the notions of complementarity and causality, Dialectica 2: 312-319. "As a more appropriate way of ... For example, it allows one to compute the probability of finding an electron in a particular region around the nucleus at a ... But what the system actually does is not determined ..." *^ Messiah, A. (1961). Quantum Mechanics, volume 1, translated by G.M ...
State of emergency
He or she must be brought before a court within at most ten days, for the court to determine whether the detention is necessary ... However, the minister of civil defence or local mayor may lift the state of emergency after an initial review of the region's ... Concordance and Complementarity of the Various Norms of International Law (cf. "Question of Human Rights and State of Emergency ... In regards to Emergency Management, regions (usually on a local government area basis) that have been affected by a natural ...
At the ends of the linear chromosomes are specialized regions of DNA called telomeres. The main function of these regions is to ... Cell-free fetal DNA is found in the blood of the mother, and can be sequenced to determine a great deal of information about ... As a result of this base pair complementarity, all the information in the double-stranded sequence of a DNA helix is duplicated ... Regions of DNA sequence that have the characteristic patterns associated with protein- or RNA-coding genes can be identified by ...
Introns make up 26% and intergenic regions 47% of the genome. Many genes are arranged in clusters and how many of these are ... In 2003, the genome sequence of the related nematode C. briggsae was also determined, allowing researchers to study the ... "The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14". Cell. 75 (5): 843-54. ... The male then probes the region with his spicules to locate the vulva, inserts them, and releases sperm. ...
In biology, the active site is the region of an enzyme where substrate molecules bind and undergo a chemical reaction. The ... Preorganised active site complementarity to the transition stateEdit. This theory is a little similar to the Lock and Key ... This specificity is determined by the arrangement of amino acids within the active site and the structure of the substrates. ... The easiest way to distinguish between them is to check whether the reaction rate is determined by the concentrations of the ...
The "black morph" variety is generally rare, except along the coastal regions of South Africa, including the Cape ... Competition with Egyptian geese Alopochen aegyptiaca overrides environmental factors in determining productivity of Black ... "Pair complementarity influences reproductive output in the polymorphic black sparrowhawkAccipiter melanoleucus". Journal of ... winter rainfall region), or other possible sources of stress in their newly urbanised environments. This is probably due to the ...
Early Islamic philosophy
Together the sira and hadith constitute the sunnah and are validated by isnad ("backing") to determine the likely truth of the ... Convergence and Complementarity (PDF), Al-Akhawayn University, archived from the original (PDF) on February 27, 2008, retrieved ... though logic continued to be studied in some Islamic regions such as Persia and the Levant. ...
Presbyterian Church (USA)
... region to region. Typical Presbyterian Church USA Order of Worship would look like this. This is taken from Madison Avenue ... The purpose of A Formula of Agreement is to elucidate the complementarity of affirmation and admonition as the basic principle ... The General Assembly of the Presbyterian Church (USA) determines and approves ecumenical statements, agreements, and maintains ... Presbyteries are organized within a geographical region to form a synod. Each synod contains at least three presbyteries, and ...
Extended mind thesis
Thus, examples such as calculators, pencils, should be considered in parallel with neural regions. Simply looking at the part ... "complementarity" of internal and external elements of cognitive systems or processes. This version might be understood as ... the EMT has the implication that some parts of a person's identity can be determined by their environment. ...
Chinese folk religion
Described as Taiji (the "Great Pole"), they represent the polarity and complementarity that enlivens the cosmos. They can ... Do, Thien (2003). Vietnamese Supernaturalism: Views from the Southern Region. Anthropology of Asia. Routledge. ISBN 978- ... It determines fate, as written in Zhou texts: "on the doer of good, heaven sends down all blessings, and on the doer of evil, ... On the contrary, the revival of folk religions in the Delta region is serving as a countervailing re-embedding force from the ...
抗體 - 維基百科，自由的百科全書
Complementarity Determining Region，CDR），也叫做高變區。喬西亞（Chothia）等人，以及後來的諾斯等人對互補決定區的結構進行了歸類。在免疫網絡理論中，每個抗體的互補決定區又被稱為獨特型或者基因型。適 ... Y形結構的基座的作用是調節免疫細胞的活動，該區域被稱為Fc區(Fragment crystallizable region，可結晶區域片段)，由兩條重鏈組成。根據抗體
سیاهچاله کلانجرم - ویکیپدیا، دانشنامهٔ آزاد
It was determined to be hydrogen emission lines that had been red shifted, indicating the object was moving away from the Earth ... Arthur M. Wolfe and Geoffrey Burbidge noted in 1970 that the large velocity dispersion of the stars in the nuclear region of ... The rate of light variations of the source, dubbed a quasi-stellar object, or quasar, suggested the emitting region had a ... January 1995). "Evidence for a black hole from high rotation velocities in a sub-parsec region of NGC4258". Nature. 373 (6510 ...
Complementarity-determining region - Wikipedia
Complementarity-determining regions (CDRs) are part of the variable chains in immunoglobulins (antibodies) and T cell receptors ... Complementarity+determining+regions at the US National Library of Medicine Medical Subject Headings (MeSH) PyIgClassify -- ... Framework region Hypervariable region Abbas AK and Lichtman AH (2003). Cellular and Molecular Immunology (5th ed.). Saunders, ... these regions are sometimes referred to as hypervariable regions. Within the variable domain, CDR1 and CDR2 are found in the ...
Characterization of the Human Ig Heavy Chain Antigen Binding Complementarity Determining Region 3 Using a Newly Developed...
... the H chain complementarity determining region 3 (CDR3H)3 is the most diverse region in the Ig molecule. Structurally, the CDR3 ... Third complementarity-determining region of mutated VH immunoglobulin genes contains shorter V, D, J, P, and N components than ... IgM heavy chain complementarity-determining region 3 diversity is constrained by genetic and somatic mechanisms until two ... Restricted utilization of germ-line VH3 genes and short diverse third complementarity-determining regions (CDR3) in human fetal ...
The V beta complementarity determining region 1 of a major histocompatibility complex (MHC) class I-restricted T cell receptor...
The V beta complementarity determining region 1 of a major histocompatibility complex (MHC) class I-restricted T cell receptor ... The V beta complementarity determining region 1 of a major histocompatibility complex (MHC) class I-restricted T cell receptor ... We investigated the role of the complementarity determining region 1 (CDR1) of T cell receptor (TCR) beta chain both in antigen ... These observations demonstrate the participation of the CDR1 region in the recognition of peptide/MHC class I as well as SAg/ ...
Complementarity determining region-independent recognition of a superantigen by B-cell antigen receptors of mantle cell...
Complementarity determining region-independent recognition of a superantigen by B-cell antigen receptors of mantle cell ... Complementarity determining region-independent recognition of a superantigen by B-cell antigen receptors of mantle cell ... Complementarity determining region-independent recognition of a superantigen by B-cell antigen receptors of mantle cell ... can be activated by complementarity determining region (CDR)-mediated interactions with a variety of autoantigens including BCR ...
The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing...
The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing ... The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing ... The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing ... The Long Third Complementarity-Determining Region of the Heavy Chain Is Important in the Activity of the Broadly Neutralizing ...
A combinatorial approach for the design of complementarity-determining region-derived peptidomimetics with in vitro anti...
... we describe a new combinatorial approach for the production of peptidomimetics using the complementarity-determining regions ( ... A combinatorial approach for the design of complementarity-determining region-derived peptidomimetics with in vitro anti- ... we describe a new combinatorial approach for the production of peptidomimetics using the complementarity-determining regions ( ...
Dominance of hydrophobic reading frames in complementarity determining region 3 of variable heavy chain genes from a patient...
Comment on An antibody-based biomarker discovery method by mass spectrometry sequencing of complementarity determining regions
An antibody-based biomarker discovery method by mass spectrometry sequencing of complementarity determining regions. Anal ... An antibody-based biomarker discovery method by mass spectrometry sequencing of complementarity determining regions. ... An antibody-based biomarker discovery method by mass spectrometry sequencing of complementarity determining regions ...
Early establishment of diverse T cell receptor profiles for influenza-specific CD8+CD62Lhi memory T cells | PNAS
complementarity-determining region;. TCM,. central memory T cell;. TEM,. effector memory T cell;. DC,. dendritic cell.. ... Y-specific CD62Lhi and CD62Llo memory T cell clonotypes defined by sequence variation in the complementarity-determining region ... that determines the nature of the primary response is likely to be both established early and limited in extent. Furthermore, ... that determine the nature of the antigenic experience. The dendritic cell (DC)-derived inflammatory cytokines IL-7 and IL-15 ( ...
Structural basis for autoantibody recognition of phosphatidylserine-β2 glycoprotein I and apoptotic cells | PNAS
... to Arg was modeled with the combined algorithm for antibody framework alignment and complementarity-determining region (CDR)- ... The initial interaction with a helper T cell then may determine the direction in which B cell specificity may evolve (47), the ... In addition to Arg-53, Arg residues at other sites within the CDR1, CDR2, and one unique location in the third framework region ... 4B), and annexin V-positive and negative cells were analyzed separately to determine the extent of scFv binding and PI staining ...
Complementarity determining regions of an anti-prion protein scFv fragment orchestrate conformation specificity and antiprion...
Complementarity determining regions of an anti-prion protein scFv fragment orchestrate conformation specificity and antiprion ... Complementarity determining regions of an anti-prion protein scFv fragment orchestrate conformation specificity and antiprion ... A peptide corresponding to the complementarity determining region 3 of the heavy chain (CDR3H) selectively bound PrPSc but had ... Prions, Monoclonal antibody, Conformation specificity, Complementarity determining regions, Recombinant antibodies, Retro- ...
Glossary of biotechnology and genetic engineering
CDR (complementarity-determining regions) These are regions of the variable (V) regions of light and heavy antibody chains that ... adjacent regions of the genome to produce a continuous nucleotide sequence across a chromosomal region. See mapping. ... crown The region at the base of the stem of cereals and forage species from which tillers or branches arise. In woody plants, ... comparative positional candidate gene A gene that is likely to be located in the same region as a DNA marker that has been ...
Category:Immunology - Wikimedia Commons
Category:Immunology - Wikimedia Commons
Appendix C: Abbreviations | Emerging Technologies for Nutrition Research: Potential for Assessing Military Performance...
JCI - Homologous regions of autoantibody heavy chain complementarity-determining region 3 (H-CDR3) in patients with pemphigus...
Homologous regions of autoantibody heavy chain complementarity-determining region 3 (H-CDR3) in patients with pemphigus cause ... usage and heavy chain complementarity-determining region 3 (H-CDR3) regions of these cloned mAbs to establish whether there are ... However, the heavy chain complementarity-determining region 3 (H-CDR3) of most pathogenic, but not nonpathogenic, mAbs shared ... Randomization of the long H-CDR3 region demonstrates the importance of W for pathogenicity. To determine whether the H-CDR3 ...
JCI - Homeostatic control of immunity by TCR peptide-specific Tregs
TCR peptides derived mostly from the conserved complementarity determining region (CDR) and framework (Fr) regions of the TCR V ... Nonstandard abbreviations used: CDR, complementarity determining region; EAE, experimental autoimmune encephalomyelitis; Fr, ... β framework or in the CDR1/2 regions. Examination of these determinants in the Fr3 and CDR2 regions of the Vβ8.2 chain suggests ... it is clear that peptides from 2 distinct conserved regions, namely the Fr3 and CDR1/2 regions on the TCR Vβ8.2 chain, can ...
Melanoma-Targeted Chemothermotherapy and In Situ Peptide Immunotherapy through HSP Production by Using Melanogenesis Substrate,...
A LAIR1 insertion generates broadly reactive antibodies against malaria variant antigens | Nature
... antibodies with broad reactivity against Plasmodium falciparum antigens from two subjects living in a malaria-endemic region in ... a, Donor C. b, Donor D. Putative complementarity-determining regions (CDRs) are highlighted in red. Dots indicate positions ... Complementarity-determining regions (CDRs) are highlighted in red. Dots indicate positions where the nucleotide of a mature ... c, Alignment of a region of antibody-encoding DNA (chromosome 14) with the corresponding region of chromosome 13 from gDNA. The ...
Profound alteration in an alpha-beta t-cell antigen receptor repertoire due to polymorphism in the 1st complementarity...
Fab fragment and that the regions of the TCR homologous to the three immunoglobulin complementarity-determining regions (CDRs 1 ... in an alpha-beta t-cell antigen receptor repertoire due to polymorphism in the 1st complementarity-determining region of the ... regions are strongly conserved in the V alpha and V beta proteins of the alpha beta T-cell antigen receptor (TCR alpha beta). ... further evidence that conventional antigen-MHC recognition and superantigen recognition are mediated by distinct regions of the ...
CN 103,555,733 A - Synthetic immunoglobulin domains with binding properties engineered in regions of the molecule different...
... an immunoglobulin comprising at least one modification in a structural loop region of said immunoglobulin and determining the ... prising at least one structural loop region, - modifying at least one nucleotide residue of at least one of said structural ... determining whether said modified immunoglobulin binds to said epitope, as well as modified immunoglobulins. ... loop regions, - transferring said modified nucleic acid in an expression system, - expressing said modified immunoglobulin, - ...
3AUV | PREDICTING AMINO ACID PREFERENCES IN THE COMPLEMENTARITY DETERMINING REGIONS OF AN ANTIBODY-ANTIGEN RECOGNITION...
Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear...
Complementarity-determining region 3 analysis of T cell receptor beta chain variable region in peripheral blood mononuclear ... To analyze the drift of the complementarity-determining region 3 (CDR3) of T cell receptor beta (TCRbeta) chain variable region ... Cells, Cultured , Complementarity Determining Regions , Genetics , Genetic Drift , Humans , Interleukin-2 , Metabolism , ... Complementarity-determining region 3 analysis of T cell receptor beta chain variable regio ...
Molecular features of the complementarity determining region 3 motif of the T cell population and subsets in the blood of...
The molecular features of the TCRBV CDR3 motif were determined using GMSP analysis; the TCRBV families were cloned and ... Yang, J., He, J., Lu, H. et al. Molecular features of the complementarity determining region 3 motif of the T cell population ... Molecular features of the complementarity determining region 3 motif of the T cell population and subsets in the blood of ... T cells of different specificity express different complementarity determining region 3 (CDR3) that vary in length or sequence ...
Chapter 5 Immunology
li,,/ul,,ul,,li,Loops = Ag-binding site ; complementarity-determining regions ( CDR1,CDR2,CDR 3) ,/li,,/ul, ... Organization of TCR and chains ,ul,,li,TCR and chains ,/li,,/ul,,ul,,ul,,li,V regions and C regions. ,/li,,/ul,,/ul,,ul ... li,,/ul,,ul,,li,Ig functions ,/li,,/ul,,ul,,ul,,li,Recognition (Fab region) ,/li,,/ul,,/ul,,ul,,ul,,li,Effector (Fc region) ,/ ... Each chain has variable region (binds Ag) and a constant region just like Igs . ,/li,,/ul,,/ul, ...
One-Step Recovery of scFv Clones from High-Throughput Sequencing-Based Screening of Phage Display Libraries Challenged to Cells...
This method was applied to some scFv fragments, characterized by heavy-chain complementarity determining regions 3 (HCDR3) of ... Light chain variable region.. Conflict of Interests. The authors declare that there is no conflict of interests regarding the ... Figure 3(b) shows that the isolated VH and VL regions of these clones actually generate full antibodies. They were also tested ... Sanger sequencing of the recovered 3_2, 3_5, and 3_67 clones confirmed 100% identity of the VH regions to the HTS data for each ...
JCI - In vivo correction of ZAP-70 immunodeficiency by intrathymic gene transfer
The Immunoscope method is based on an RT-PCR of the hypervariable complementarity determining region 3 (CDR3), which allows the ... Nonstandard abbreviations used: ADA, adenosine deaminase; BV, β chain hypervariable region; CDR3, complementarity determining ... LTR deleted of 400 bp in the U3 region (29); U3, untranslated 3′ region of the LTR; and R, repeated region of the LTR. ... B) The activation status of the T cell population was determined using PE-conjugated α-CD25 and α-CD69 mAbs, and the relative ...
CD4 T Cells Selected by Antigen Under Th2 Polarizing Conditions Favor an Elongated TCRα Chain Complementarity-Determining...
Germline-Encoded Affinity for Cognate Antigen Enables Vaccine Amplification of a Human Broadly Neutralizing Response against...
- Complementarity-determining regions (CDRs) are part of the variable chains in immunoglobulins (antibodies) and T cell receptors, generated by B-cells and T-cells respectively, where these molecules bind to their specific antigen. (wikipedia.org)
- Since most sequence variation associated with immunoglobulins and T cell receptors are found in the CDRs, these regions are sometimes referred to as hypervariable regions. (wikipedia.org)
- Structurally, the CDR3 H is in the center of the Ag binding site, interacting directly with the other CDRs and framework regions both from H and L chains, as well as with the Ag itself ( 1 , 2 , 3 , 4 , 5 ). (jimmunol.org)
- Here, we describe a new combinatorial approach for the production of peptidomimetics using the complementarity-determining regions (CDRs) from gastrin17 (pyroEGPWLEEEEEAYGWMDF-NH(2)) antibodies as starting material for cyclic peptide synthesis in a microarray format. (metabolomicscentre.nl)
- Consequently, it has been proposed that TCR alpha beta has a conformation similar to that of an immunoglobulin Fab fragment and that the regions of the TCR homologous to the three immunoglobulin complementarity-determining regions (CDRs 1, 2, and 3) bind to the peptide antigen-major histocompatibility complex (MHC) molecule ligand. (scripps.edu)
- Antibody paratopes are formed by hypervariable complementarity-determining regions (CDRH3s) and variable gene-encoded CDRs. (nih.gov)
- All natural antibodies have six loops, called complementarity-determining regions(CDRs), performing this function. (newscientist.com)
- The first and second complementarity determining regions (CDR1 and CDR2) of this Vα are shorter than the CDRs corresponding to the majority of other Vα gene families, and three-dimensional structures of CDRs of these lengths have not been described previously. (soton.ac.uk)
- The majority of the plasmablast-derived antibodies contained higher numbers of variable region somatic hypermutations and evidence for selection, as demonstrated by replacement to silent ratio's (R/S) greater than 2.9 in the complementarity-determining regions (CDRs). (biomedcentral.com)
- Complementarity determining regions (CDRs) are underlined. (unl.edu)
- A minimal format antibody fragment is the single VH or VL domain carrying three hyper-variable complementarity-determining regions (CDRs), which has proven sufficient for antigen recognition [ 4 ]. (mdpi.com)
- The complementarity-determining regions (CDRs) sequence of the E6 TCR was determined. (cancer.gov)
- The variable domain of both the TCR α-chain and β-chain each have three hypervariable or complementarity determining regions (CDRs). (wikipedia.org)
- These studies indicate that H-CDR3 is critical for pathogenicity of a human autoantibody, that a small region (even 1 amino acid) can mediate pathogenicity, and that pathogenicity can be uncoupled from binding in these antibodies. (jci.org)
- The five different types of Fc regions allow antibodies to be grouped into five isotypes . (wikipedia.org)
- Though the general structure of all antibodies is very similar, a small region at the tip of the protein is extremely variable, allowing millions of antibodies with slightly different tip structures, or antigen-binding sites, to exist. (wikipedia.org)
- To characterize the plasmablast B cell response to the pneumococcal conjugated vaccine, the germline usage and the variable region somatic hypermutations on these antibodies were analyzed. (biomedcentral.com)
- The complementarity determining region (CDR) of antibodies has a highly specific sequence for each antigen . (news-medical.net)
- Frequency of antibodies expressing a given number of Isoleucine (I) or Leucine (L) residues in the complementarity determining region (CDR). (news-medical.net)
- Many groups have focused on the sequence of the V region genes of antinuclear antibodies and several features accounting for DNA binding have emerged from these sequences, especially in VH-CDR (H3). (biomedcentral.com)
- The membrane-proximal region of the ectodomain of the gp41 envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1) is the target of three of the five broadly neutralizing anti-HIV-1 antibodies thus far isolated. (rcsb.org)
- We have determined crystal structures of the antigen-binding fragment for one of these antibodies, 2F5, in complex with 7-mer, 11-mer, and 17-mer peptides of the gp41 membrane-proximal region, at 2.0-, 2.1-, and 2.2-A resolutions, respectively. (rcsb.org)
- Each monoclonal antibody (mAb) sequence is a novel protein that requires de novo sequencing with no resembling proteins (for the variable regions) in the databases. (nature.com)
- The antibody recognizes a unique molecule of the pathogen, called an antigen , via the Fab's variable region . (wikipedia.org)
- The ability of an antibody to communicate with the other components of the immune system is mediated via its Fc region (located at the base of the "Y"), which contains a conserved glycosylation site involved in these interactions. (wikipedia.org)
- Each Fc region of a particular antibody isotype is able to bind to its specific Fc Receptor (except for IgD, which is essentially the BCR), thus allowing the antigen-antibody complex to mediate different roles depending on which FcR it binds. (wikipedia.org)
- The ability of an antibody to bind to its corresponding FcR is further modulated by the structure of the glycan(s) present at conserved sites within its Fc region. (wikipedia.org)
- 1. An isolated polynucleotide encoding a humanized antibody comprising a light chain variable region comprising the amino acid sequence of SEQ. (google.co.uk)
- NO:7 and a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:9, wherein said humanized antibody binds prostate stem cell antigen (PSCA), and wherein administration of said humanized antibody to a subject diagnosed with prostate cancer decreases tumor growth to a greater extent than treatment with the murine 1G8 monoclonal antibody. (google.co.uk)
- Our recent technology development has provided a set of simple protocols that allow development of single antibody variable (V) region domains that can function inside the reducing environment of the cell. (mdpi.com)
- Without determining the difference between leucine and isoleucine, how would a researcher determine that an antibody works in practice? (news-medical.net)
- The human sequences were derived from the constant domains of human IgG1 and the variable framework regions of the Eu myeloma antibody. (rxlist.com)
- The murine sequences were derived from the complementarity-determining regions of a murine anti-Tac antibody. (rxlist.com)
- Contacts are made with five complementarity-determining regions of the antibody as well as with nonpolymorphic regions. (rcsb.org)
- Biochemical studies with proteoliposomes confirm the importance of lipid membrane and hydrophobic context in the binding of 2F5 as well as in the binding of 4E10, another broadly neutralizing antibody that recognizes the membrane-proximal region of gp41. (rcsb.org)
- We used complementarity determining region sequences from 8708 and 8709 Fabs to generate an anti-EGFR IgG and (scFv) 2 and scFv-Fc antibody fragments. (thno.org)
- Humanized antibody against a-actin, characterized by comprising the murine variable region which consists of SEQ ID NO: X and human y chain regions. (mondaq.com)
- Despite its crucial role in determining the nature and specificity of the Ag binding capability of the Ab molecule, the human CDR3 H has not yet been fully characterized. (jimmunol.org)
- The variable regions (VHH) in these heavy chain-only Abs demonstrate comparable affinity and specificity for antigens to conventional immunoglobulins despite their much smaller size. (rupress.org)
- Crystal structures reveal that the specificity differences are primarily mediated by complementarity-determining region H3 (CDR H3). (rcsb.org)
- Specificity of scFv fragments against Cap-PCV2 and complementarity determining regions (CDR). (springer.com)
- Within the variable domain, CDR1 and CDR2 are found in the variable (V) region of a polypeptide chain, and CDR3 includes some of V, all of diversity (D, heavy chains only) and joining (J) regions. (wikipedia.org)
- In the productive repertoire, the H chain complementarity determining region 3 (CDR3 H ) was significantly shorter (46.7 ± 0.5 nucleotides) than in the nonproductive repertoire (53.8 ± 1.9 nucleotides) because of the tendency to select rearrangements with less TdT activity and shorter D segments. (jimmunol.org)
- Analysis of the human CDR3 H with JOINSOLVER has provided comprehensive information on the influences that shape this important Ag binding region of V H chains. (jimmunol.org)
- Located at the joining of the V H , D, and J H segments, the H chain complementarity determining region 3 (CDR3 H ) 3 is the most diverse region in the Ig molecule. (jimmunol.org)
- One reason for this lack of information about the CDR3 H relates to the difficulty in analyzing the sequences of this highly diverse region. (jimmunol.org)
- However, the heavy chain complementarity-determining region 3 (H-CDR3) of most pathogenic, but not nonpathogenic, mAbs shared an amino acid consensus sequence. (jci.org)
- To analyze the drift of the complementarity-determining region 3 (CDR3) of T cell receptor beta (TCRbeta) chain variable region in T cells of healthy volunteers cultured with interleukin-2 ( IL-2 ). (bvsalud.org)
- CDR3 of the Vα85.33 domain is highly flexible and this is consistent with plasticity of this region of the TCR. (soton.ac.uk)
- These are also called complementarity determining regions (CDR1, CDR2 and CDR3). (news-medical.net)
- The central third complementarity-determining region (CDR3) residues of a human monoclonal anti. (biomedcentral.com)
- The IMGT unique numbering for V-DOMAIN provides a standardized numbering for the CDR3-IMGT and FR4-IMGT of rearranged V-J-REGION and V-D-J-REGION ( IMGT Scientific chart ). (imgt.org)
- CDR3) which consist of SEQ ID NO: X, SEQ ID NO: Y and SEQ ID NO: Z in the light chain and SEQ ID NO: A, SEQ ID NO: B and SEQ ID NO: C in the heavy chain and human y chain regions. (mondaq.com)
- We investigated the role of the complementarity determining region 1 (CDR1) of T cell receptor (TCR) beta chain both in antigen/major histocompatibility complex I (MHC I) and in superantigen (SAg)/MHC II complex recognition. (rupress.org)
- These observations demonstrate the participation of the CDR1 region in the recognition of peptide/MHC class I as well as SAg/MHC II complexes. (rupress.org)
- The results confirm the importance of the V beta CDR1 in antigen-MHC molecule recognition, supporting the Fab-like structural model of TCR alpha beta, and provide further evidence that conventional antigen-MHC recognition and superantigen recognition are mediated by distinct regions of the TCR beta chain. (scripps.edu)
- The prevalence of a common point mutation, previously identified in the CDR1 gene of some ITS genotype 1 isolates, was determined for avian and human C. (thefreedictionary.com)
- A crystal structure of a complex of 2F5 and a peptide corresponding to its core epitope on gp41, ELDKWAS, revealed that the peptide interacts with residues at the base of the unusually long (22-residue) third complementarity-determining region of the heavy chain (CDR H3) but not the apex. (asm.org)
- Higher relative affinity for DOPS-β2GPI was achieved by the introduction of Arg residues into the 3H9 H chain variable region at positions previously shown to mediate DNA binding. (pnas.org)
- Amino acid residues that are critical in maintaining the framework structure of immunoglobulin heavy- and light-chain variable (V) regions are strongly conserved in the V alpha and V beta proteins of the alpha beta T-cell antigen receptor (TCR alpha beta). (scripps.edu)
- We have observed that Ile and Leu structural differences can affect the antibody's binding affinity, and in some cases, when incorrect Ile or Leu residues are in or around CDR regions, binding no longer occurs. (news-medical.net)
- The residues in these variable domains are located in two regions of the TCR, at the interface of the α- and β-chains and in the β-chain framework region that is thought to be in proximity to the CD3 signal-transduction complex. (wikipedia.org)
- This method is usually straightforward in the V H and J H regions, where there are large regions of sequence similarity. (jimmunol.org)
- The core epitope of 2F5, the most studied of the two MAbs, has been defined conveniently by a short linear sequence, ELDKWA, which is found at the extreme C-terminal end of the C-heptad repeat region on the ectodomain of gp41 ( 37 ). (asm.org)
- A separate set of mouse experiments used adoptively transferred TCRβ transgenic lymphocytes, then analyzed H-Y-specific CD62L hi and CD62L lo memory T cell clonotypes defined by sequence variation in the complementarity-determining region (CDR) 3α ( 14 ). (pnas.org)
- CAAT box (also CAT box) A conserved sequence found within the promoter region of the protein-encoding genes of many eukaryotic organisms. (fao.org)
- In this context, algorithms and tools are necessary for analyzing the IG and TR diversity on nucleotide as well as on amino acid sequence level, identifying highly proliferated clonotypes, determining the diversity of the cell repertoire found in a sample, comparing different states of the human immune system, and visualizing all relevant information. (biomedcentral.com)
- Thus, while V H 6-1 provides a framework for development of HA stem-directed bnAbs, sequence differences in CDR H3 junctional regions during VDJ recombination can alter reactivity and evolutionary pathways toward increased breadth. (rcsb.org)
- The V beta complementarity determining region 1 of a major histocompatibility complex (MHC) class I-restricted T cell receptor is involved in the recognition of peptide/MHC I and superantigen/MHC II complex. (rupress.org)
- These enhanced properties of Ac-6-FP were attributable to structural alterations in MR1 that subsequently affected MAIT TCR recognition via conformational changes within the complementarity-determining region (CDR) 3? (jove.com)
- The E6 TILs recognized the cells expressing E6 but not similar cells expressing only green fluorescent protein, suggesting the cells targeted a naturally processed region of E6 and that recognition might be HLA-A*02:01 restricted. (cancer.gov)
- Framework region Hypervariable region Abbas AK and Lichtman AH (2003). (wikipedia.org)
- IMEX is an user-friendly and flexible framework for performing clonality experiments based on CDR and V-(D)-J rearranged regions, diversity analysis, primer efficiency, and various different visualization experiments. (biomedcentral.com)
- The heavy chain variable region (VH) segments mainly used in this technology are based on a designer framework that folds inside cells without the need for the intra-chain disulphide bond and can be used as drug surrogates to determine on-target effects (target validation) and as templates for small molecule drug development. (mdpi.com)
- The Complementarity Determining Regions (CDR) and the Framework Regions (FR) are parts of the variable regions of the Immunoglobulin and T cell Receptor chains. (imgt.org)
- The IMGT unique numbering for V-REGION  has allowed to redefine the limits of the Complementarity Determining Regions and Framework Regions, designated as CDR-IMGT and FR-IMGT , respectively in IMGT. (imgt.org)
- The complementarity-determining regions of Ibritumomab bind to the CD20 antigen on B lymphocytes. (centerwatch.com)
- Unique sdAbs exhibiting nanomolar or better affinities for the recombinant proteins were obtained and most of the isolated sdAbs retained their ability to bind antigen after cycles of heating as determined by enzyme linked immunosorbent assay (ELISA). (mdpi.com)
- A peptide corresponding to the complementarity determining region 3 of the heavy chain (CDR3H) selectively bound PrPSc but had lost antiprion activity. (uni-bayreuth.de)
- The Constant region is proximal to the cell membrane, followed by a transmembrane region and a short cytoplasmic tail, while the Variable region binds to the peptide/MHC complex. (wikipedia.org)
- 10 . The isolated nucleic acid of claim 9 , wherein one or more canonical glycosylation sites are absent from the V1V2 region of the gp120 molecule. (google.com)
- In addition, D and J elements of the IgH chains encode the complementarity determining region (CDR) 3 that constitutes a significant part of the Ig antigen binding site. (biomedcentral.com)
- A light chain can be of either the lambda (IGL) or kappa (IGK) variety, whereas the heavy chain has only one possibility (IGH), and the loci encoding these three chains reside in distinct regions of the genome. (biomedcentral.com)
- The average length of H3 loops for guinea pigs has not been determined. (asm.org)
- Also included is a method of achieving systemic circulation of a TNFα inhibitor in a subject comprising administering the TNFα inhibitor to the central lung region or the peripheral lung region of the subject via inhalation, such that systemic circulation of the TNFα inhibitor is achieved. (freepatentsonline.com)
- 11. The method of any one of claims 1- 8 , wherein the TNFα inhibitor is distributed to the peripheral lung region of the subject such that a P/C ratio of about 1.3 is achieved. (freepatentsonline.com)
- IgE's Fab paratope binds to allergic antigen , for example house dust mite particles, while its Fc region binds to Fc receptor ε. (wikipedia.org)
- Figure 1 shows the configuration of whole IgG together with single-chain Fv (scFv), which consists of heavy (H) and light (L) chain variable regions (VH and VL) joined in a single polypeptide by a flexible linker to improve its assembly inside cells. (mdpi.com)
- IRDye680RD-labeled 8709 scFv-Fc and IRDye800CW-labeled nimotuzumab used in combination were able to determine the ratio of cells expressing EGFR and a deletion mutant EGFR vIII . (thno.org)
- A) Binding capacity of scFv fragments to Cap-PCV2 and BSA determined by ELISA. (springer.com)
- allergen, tumor associated antigen, autoantigen, enzyme, bacterial antigens, fungal antigen, virus antigen and protozoon antigen, the immunoglobulin (Ig) that wherein comprises unmodified structure ring region is at least one molecule described in specific binding not. (rpxcorp.com)
- One interpretation of the inability to elicit 2F5-like Abs in animals is that it may be difficult to elicit an Ab with a very long third complementarity-determining region of the heavy chain (CDR H3), as is found in 2F5. (asm.org)
- This gene segment was contained in a 5.0 kb EcoRI fragment isolated by a 700 bp probe 3' to the mouse heavy chain joining minigene region between the XbaI and EcoRI sites. (unl.edu)
- Seal SN, Monestier M, Radic MZ: Diverse roles for the third complementarity determining region of the heavy chain (H3) in the binding of immunoglobulin Fv fragments to DNA, nucleosomes and cardiolipin. (biomedcentral.com)
- Each chain is composed of two extracellular domains: Variable (V) region and a Constant (C) region, both of Immunoglobulin superfamily (IgSF) domain forming antiparallel β-sheets . (wikipedia.org)
- The capsid often determines the shape of the virus. (fao.org)
- This region represents the germline organization of the T cell receptor beta locus. (cancerindex.org)
- To observe anti-cancer effects of Jianpi Jiedu Recipe (JJR) on liver cancer (LC) rats with Pi deficiency syndrome (PDS) and its relation with the third complementary-determining region gene spectratyping of TCRVβ-chain (TCRVβCDR3). (cancerindex.org)
- No switching of constant-region isotype. (slideshare.net)
- 2010). Despite the diversity of structures and distributions, characterised silks have certain features in common, notably, being semicrystalline materials, that is materials with regions of ordered molecular structure (crystallites) within an amorphous matrix and also, all show typically similar protein compositions, often rich in alanine, serine, and/or glycine (Sutherland et al. (wipo.int)
- the nucleic acid that provides encoded packets to contain the immunoglobulin (Ig) at least one structure ring region,-modify at least one nucleotide residue in structure ring region described at least one,-described modified nucleic acid is transferred to expression system,-express described modified immunoglobulin (Ig),-the modified immunoglobulin (Ig) of expressing is contacted with epi-position, and-whether measure described modified immunoglobulin (Ig) in conjunction with described epi-position. (rpxcorp.com)
- Large number genetically determined variants in human population. (slideshare.net)