Complement Fixation Tests: Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1.Tropical Medicine: The branch of medicine concerned with diseases, mainly of parasitic origin, common in tropical and subtropical regions.Schistosoma mansoni: A species of trematode blood flukes of the family Schistosomatidae. It is common in the Nile delta. The intermediate host is the planorbid snail. This parasite causes schistosomiasis mansoni and intestinal bilharziasis.Schistosomiasis mansoni: Schistosomiasis caused by Schistosoma mansoni. It is endemic in Africa, the Middle East, South America, and the Caribbean and affects mainly the bowel, spleen, and liver.Schistosomicides: Agents that act systemically to kill adult schistosomes.Schistosomiasis: Infection with flukes (trematodes) of the genus SCHISTOSOMA. Three species produce the most frequent clinical diseases: SCHISTOSOMA HAEMATOBIUM (endemic in Africa and the Middle East), SCHISTOSOMA MANSONI (in Egypt, northern and southern Africa, some West Indies islands, northern 2/3 of South America), and SCHISTOSOMA JAPONICUM (in Japan, China, the Philippines, Celebes, Thailand, Laos). S. mansoni is often seen in Puerto Ricans living in the United States.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Liver Diseases, Parasitic: Liver diseases caused by infections with PARASITES, such as tapeworms (CESTODA) and flukes (TREMATODA).Brucellosis, Bovine: A disease of cattle caused by bacteria of the genus BRUCELLA leading to abortion in late pregnancy. BRUCELLA ABORTUS is the primary infective agent.Oxamniquine: An anthelmintic with schistosomicidal activity against Schistosoma mansoni, but not against other Schistosoma spp. Oxamniquine causes worms to shift from the mesenteric veins to the liver where the male worms are retained; the female worms return to the mesentery, but can no longer release eggs. (From Martindale, The Extra Pharmacopoeia, 31st ed, p121)TexasAntibody Formation: The production of ANTIBODIES by proliferating and differentiated B-LYMPHOCYTES under stimulation by ANTIGENS.Antibodies, Bacterial: Immunoglobulins produced in a response to BACTERIAL ANTIGENS.HistoplasminImmunodiffusion: Technique involving the diffusion of antigen or antibody through a semisolid medium, usually agar or agarose gel, with the result being a precipitin reaction.Hemagglutination Tests: Sensitive tests to measure certain antigens, antibodies, or viruses, using their ability to agglutinate certain erythrocytes. (From Stedman, 26th ed)Complement System Proteins: Serum glycoproteins participating in the host defense mechanism of COMPLEMENT ACTIVATION that creates the COMPLEMENT MEMBRANE ATTACK COMPLEX. Included are glycoproteins in the various pathways of complement activation (CLASSICAL COMPLEMENT PATHWAY; ALTERNATIVE COMPLEMENT PATHWAY; and LECTIN COMPLEMENT PATHWAY).Complement Activation: The sequential activation of serum COMPLEMENT PROTEINS to create the COMPLEMENT MEMBRANE ATTACK COMPLEX. Factors initiating complement activation include ANTIGEN-ANTIBODY COMPLEXES, microbial ANTIGENS, or cell surface POLYSACCHARIDES.Hepatitis, Infectious Canine: A contagious disease caused by canine adenovirus (ADENOVIRUSES, CANINE) infecting the LIVER, the EYE, the KIDNEY, and other organs in dogs, other canids, and bears. Symptoms include FEVER; EDEMA; VOMITING; and DIARRHEA.Coyotes: The species Canis latrans in the family CANIDAE, a smaller relative of WOLVES. It is found in the Western hemisphere from Costa Rica to Alaska.Adenoviruses, Canine: Species of the genus MASTADENOVIRUS that causes fever, edema, vomiting, and diarrhea in dogs and encephalitis in foxes. Epizootics have also been caused in bears, wolves, coyotes, and skunks. The official species name is Canine adenovirus and it contains two serotypes.Wolves: Any of several large carnivorous mammals of the family CANIDAE that usually hunt in packs.Hepatitis C, Chronic: INFLAMMATION of the LIVER in humans that is caused by HEPATITIS C VIRUS lasting six months or more. Chronic hepatitis C can lead to LIVER CIRRHOSIS.Hepatitis B, Chronic: INFLAMMATION of the LIVER in humans caused by HEPATITIS B VIRUS lasting six months or more. It is primarily transmitted by parenteral exposure, such as transfusion of contaminated blood or blood products, but can also be transmitted via sexual or intimate personal contact.Hepatitis B: INFLAMMATION of the LIVER in humans caused by a member of the ORTHOHEPADNAVIRUS genus, HEPATITIS B VIRUS. It is primarily transmitted by parenteral exposure, such as transfusion of contaminated blood or blood products, but can also be transmitted via sexual or intimate personal contact.Foxes: Any of several carnivores in the family CANIDAE, that possess erect ears and long bushy tails and are smaller than WOLVES. They are classified in several genera and found on all continents except Antarctica.Hepatitis C: INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.Anaplasmosis: A disease of cattle caused by parasitization of the red blood cells by bacteria of the genus ANAPLASMA.Phenol: An antiseptic and disinfectant aromatic alcohol.Anaplasma marginale: A species of gram-negative bacteria and causative agent of severe bovine ANAPLASMOSIS. It is the most pathogenic of the ANAPLASMA species.Anaplasma phagocytophilum: A species of gram-negative bacteria in the genus ANAPLASMA, family ANAPLASMATACEAE, formerly called Ehrlichia phagocytophila or Ehrlichia equi. This organism is tick-borne (IXODES) and causes disease in horses and sheep. In humans, it causes human granulocytic EHRLICHIOSIS.Anaplasma: A genus of gram-negative bacteria whose organisms are obligate parasites of vertebrates. Species are transmitted by arthropod vectors with the host range limited to ruminants. Anaplasma marginale is the most pathogenic species and is the causative agent of severe bovine anaplasmosis.Phenols: Benzene derivatives that include one or more hydroxyl groups attached to the ring structure.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Cattle Diseases: Diseases of domestic cattle of the genus Bos. It includes diseases of cows, yaks, and zebus.Ehrlichiosis: A tick-borne disease characterized by FEVER; HEADACHE; myalgias; ANOREXIA; and occasionally RASH. It is caused by several bacterial species and can produce disease in DOGS; CATTLE; SHEEP; GOATS; HORSES; and humans. The primary species causing human disease are EHRLICHIA CHAFFEENSIS; ANAPLASMA PHAGOCYTOPHILUM; and Ehrlichia ewingii.Lymphogranuloma Venereum: Subacute inflammation of the inguinal lymph glands caused by certain immunotypes of CHLAMYDIA TRACHOMATIS. It is a sexually transmitted disease in the U.S. but is more widespread in developing countries. It is distinguished from granuloma venereum (see GRANULOMA INGUINALE), which is caused by Calymmatobacterium granulomatis.Proctitis: INFLAMMATION of the MUCOUS MEMBRANE of the RECTUM, the distal end of the large intestine (INTESTINE, LARGE).Chlamydia trachomatis: Type species of CHLAMYDIA causing a variety of ocular and urogenital diseases.Rectal Diseases: Pathological developments in the RECTUM region of the large intestine (INTESTINE, LARGE).Biology: One of the BIOLOGICAL SCIENCE DISCIPLINES concerned with the origin, structure, development, growth, function, genetics, and reproduction of animals, plants, and microorganisms.Chlamydia: A genus of the family CHLAMYDIACEAE whose species cause a variety of diseases in vertebrates including humans, mice, and swine. Chlamydia species are gram-negative and produce glycogen. The type species is CHLAMYDIA TRACHOMATIS.Proctocolitis: Inflammation of the RECTUM and the distal portion of the COLON.Granuloma Inguinale: Anogenital ulcers caused by Calymmatobacterium granulomatis as distinguished from lymphogranuloma inguinale (see LYMPHOGRANULOMA VENEREUM) caused by CHLAMYDIA TRACHOMATIS. Diagnosis is made by demonstration of typical intracellular Donovan bodies in crushed-tissue smears.Ulcer: A lesion on the surface of the skin or a mucous surface, produced by the sloughing of inflammatory necrotic tissue.

Complement fixation titers in cattle following intranasal inoculation of Hemophilus somnus. (1/1682)

Five bulls were inoculated intranasally with a live culture of Hemophilus somnus originally isolated from a clinical case of Hemophilus septicemia. Preinoculation and postinoculation blood samples were taken at weekly intervals for nine weeks for measuring complement fixation titers and daily postinoculation temperatures were taken for one week. Three animals had transient fever and slight lethargy was observed in two animals had a transitory rise in complement fixation titers in the second to fifth weeks postexposure while one animal which had been seronegative on preinoculation testing produced little serological response to the organism. The experiment demonstrated that the nasal instillation of young cattle using an originally pathogenic H. somnus isolate is capable of stimulating only transitory complement fixation antibody titer.  (+info)

Complement fixing hepatitis B core antigen immune complexes in the liver of patients with HBs antigen positive chronic disease. (2/1682)

One hundred and fifty-two biopsies from serologically HBsAg positive and negative patients with liver disease were studied in immunofluorescence: for the presence of the surface (HBs) and the core (HBc) antigenic determinants foeterminants of the hepatitis B virus, of immunoglobulins and complement (C) deposits, and for the capacity to fix human C. Circumstantial evidence is presented suggesting that HBc immune-complexes are a relevant feature in the establishment and progression of chronic HBSAg liver disease. C fixation by liver cells was shown in all HBC positive patients with chronic hepatitis; an active form was present in every case, except two with a persistent hepatitis, an inverse ratio of HBc to C binding fluorescence being noted between active chronic hepatitis and cirrhotic patients. HBc without C fixation was observed in only three patients in the incubation phase of infectious hepatitis. IgG deposits were often found in HBc containing, C fixing nuclei. No C binding or IgG deposits were observed in acute self-limited type B hepatitis, in serologically positive patients with normal liver or minimal histological lesions, with and without HBs cytoplasmic fluorescence in their biopsy, or in serologically negative individuals.  (+info)

Serum dilution neutralization test for California group virus identification and serology. (3/1682)

The serum dilution neutralization test was evaluated for serological diagnosis of California group arbovirus infections and identification of virus isolates. The technical advantages and the degree of subtype specificity of the serum dilution neutralization test over the hemagglutination inhibition test and the complement fixation test were demonstrated with paired specimens from human cases, single human survey sera, and sentinel rabbit sera. Twenty-one virus isolates from various geographical areas of the United States were also used to evaluate the efficacy of the serum dilution neutralization test for specific virus identification.  (+info)

Chagas' disease diagnosis: comparative analysis of parasitologic, molecular, and serologic methods. (4/1682)

During the course of chronic chagasic infection, low parasitemia levels prevent parasite detection by current techniques such as hemoculture and xenodiagnosis. Since serologic tests have sensitivity but lack specificity, molecular assays based on the polymerase chain reaction (PCR) have been proposed as alternative tools for parasite detection in individuals with chronic Chagas' disease. A variable degree of PCR efficiency has been reported in the literature and illustrates the need for further evaluation of large numbers of chagasic patients. In this study, we compared an optimized PCR technique with hemoculture and complement-mediated lysis (CoML) in 113 individuals from or living in endemic areas of Brazil who had conventional serologic results that were either positive, negative, or inconclusive. The PCR amplification yielded positive results in 83.5% (66 of 79) of individuals with positive serology, 47.6% (10 of 21) with negative serology, and 46.2% (6 of 13) with inconclusive serology. Of 10 patients with negative serology and positive PCR result, eight (80%) had positive CoML, indicating that they could have been chagasic but were not mounting immune responses. The PCR results were also positive for all individuals who had positive hemoculture, for 37 individuals with negative hemoculture and positive serology, and for two of six individuals with inconclusive serology and negative hemoculture. Thirteen individuals living in nonendemic areas who had negative serology were used as a negative control group: 100% had negative PCR results. Our results show that the optimized PCR protocol used here was very sensitive in detecting the presence of Trypanosoma cruzi in chronic chagasic patients. The PCR and CoML results were well correlated in all of the groups studied, which suggests that our PCR protocol may be effective in the evaluation of cure in patients who receive anti-parasite treatment.  (+info)

Inhibition of K cell function by human breast cancer sera. (5/1682)

Sera from breast cancer patients and from female controls were tested for inhibition of lysis of antibody-coated target cells by human leukocytes (K cells). Sera from 39% of breast cancer patients, but from only 8% of controls, inhibited lysis by more than 30%. This inhibition was unrelated to the stage of the disease, the patient's age or whether the patient was pre- or post-operative. Inhibition was apparently not due to anti-HLA antibodies and did not correlate with the IgG level or anti-complementary activity of the serum. On fractionation by gel-filtration, inhibitory activity was found in fractions of higher molecular weight than IgG. As no IgG could be detected in these fractions, inhibition is probably not due to immune complexes containing IgG antibody. The inhibitory factor may well contribute to the immunosuppressed status of a proportion of breast cancer patients.  (+info)

Immune complexes and complement hypercatabolism in patients with leprosy. (6/1682)

The occurrence of immune complexes in the serum and the level of the C3 breakdown product C3d in the plasma from patients with leprosy were studied by quantitative methods and the results were compared in various forms of the disease. These studies were performed on sixty-two samples from twenty-six patients. The serum 125I-C1q binding activity was found to be increased by more than 2 s.d., as compared to the normal values, in most of the sera from patients with erythema nodosum leprosum (ENL) (80%) and uncomplicated lepromatous leprosy (82%), but also in the sera from patients with tuberculoid leprosy (58%). In vitro studies suggested that immune complexes involving mycobacterial antigens were present in leprosy sera. An increased C3d level (greater than 2s.d.) was also found in most of the plasma from patients with ENL (70%), but rarely in the plasma from patients with uncomplicated lepromatous leprosy (18%) and never in tuberculoid leprosy patients' plasma. The absence of a significant correlation between the 125I-C1q binding activity and the C3d level in leprosy patients may suggest that extravascular immune complexes are involved in the complement activation occurring in ENL. The quantitation of C3d in plasma may be of some practical interest in the early diagnosis of ENL complications of leprosy.  (+info)

Studies on the immunogenicity of protamines in humans and experimental animals by means of a micro-complement fixation test. (7/1682)

A complement fixation study with human, monkey and rabbit sera, using purified sperm nuclear basic proteins as antigens, led to the following conclusions. (1) Protamines, the sperm-specific basic nuclear proteins, may be immunogenic in mammalians. (2) Antibodies detected in the indirect immunofluorescence test on human swollen sperm heads in sera from infertile and vasectomized men, are directed primarily against human protamines. (3) The results obtained suggested that differences in the immunization site and/or in the configuration of the immunizing protamine, may lead to the formation of antibodies directed against different antigenic determinants. Autoimmunity to protamines, following vasectomy or in infertile men, is accompanied by the formation of antibodies cross-reacting with common antigenic determinants present in protamines of other species. Induction of immunity to protamines by means of immunization with protamines-RNA complexes (in rabbits), or protamine-insulin complexes (in humans), leads to the formation of antibodies reacting more specifically with the immunizing protamine, showing only slight cross-reaction with other protamines. (4) The histone-like fraction present in mature human spermatozoa is composed mainly of histone fraction H2B.  (+info)

Detection of core antibody in hepatitis B infection. (8/1682)

Hepatitis B core antigen (HBcAg) is found on the decoated Dane particle and on a morphologically similar particle detected mainly in the nucleus of hepatocytes of patients with hepatitis B. HBcAg prepared from the liver of a chimpanzee infected with hepatitis B virus was used to test human serum for core antibody (anti-HBc) by complement fixation. Anti-HBc was found in serum collected from patients with hepatitis B in both the acute and convalescent stages, from carriers of hepatitis B surface antigen (HBsAg) and from patients with chronic liver or renal disease who were carriers of HBsAg. It was not found in patients with hepatitis A or infectious mononucleosis, or in healthy persons who were not carriers of HBsAg.  (+info)

*Complement fixation test

The complement fixation test is an immunological medical test that can be used to detect the presence of either specific ... The basic steps of a complement fixation test are as follows: Serum is separated from the patient. Patients naturally have ... Complement Fixation Tests at the US National Library of Medicine Medical Subject Headings (MeSH) http://medmicro.atwebpages.com ... To negate any effects this might have on the test, the complement proteins in the patient's serum must be destroyed and ...

*Orbivirus

These tests include ELISAs and complement fixation tests. Lebombo virus Pata virus African horse sickness virus (Serotypes 1-9 ... The serogroups are differentiated on the basis of a fourfold or greater difference in antibody based tests. ...

*Schistosoma haematobium

Alternatively diagnosis can be made by complement fixation tests. As of 2012[update], commercial blood tests included ELISA and ... doi:10.1016/0035-9203(84)90283-9. Kinkel HF, Dittrich S, Bäumer B, Weitzel T (2012). "Evaluation of eight serological tests for ... an Indirect immunofluorescence test, but these have low sensitivity ranging from 21% to 71%. The main cause of schistomiasis is ...

*History and naming of human leukocyte antigens

Complement fixation assay. The complement fixation test was modified to assay Antiserum mediated RBC lysis. Chromium release ... A series of tests on cultured cells revealed that, within the "LA" group, a donor tissue might have some antigens but not ... By testing different anti-sera from recipients they were able to uncover some with unique reactivities. As a result, scientists ... To work around this problem a 'workshop' number "w#" was often assigned while testing continued to determine which series the ...

*Sarcocystis

Ante mortem diagnosis may be made with the use of dermal sensitivity testing or complement fixation tests. Muscle biopsy is ...

*Lymphocytic choriomeningitis

However, some authors note that such complement-fixation tests are insensitive and should not be used for diagnosis. Dr. Clare ... Blood samples were obtained and tested for LCMV antibodies. Of the 82 workers that were tested, seven had definite serologic ... Serologic testing is not recommended for pet rodents, as it has been unreliable in detecting antibodies in animals with active ... The island of Vir in Croatia is one of the biggest described endemic places of origin of LCMV in the world, with IFA testing ...

*Paracoccidioides brasiliensis

Double diffusion in agar gel and complement fixation test, are amongst the most commonly used tests in serodiagnosis. Culture ... which might provide a basis for the development of a diagnostic test. Tests targeting the presence of serum antibodies to P. ... A number of serologic tests have been employed for the diagnosis of paracoccidioidomycosis. ... "Rapid and reliable method for production of a specific Paracoccidioides brasiliensis immunodiffucsion test antigen". J. Clin. ...

*Mycoplasma hyorhinis

Bacterial culture, immunofluorescent antibody testing (IFAT), complement fixation test and haemagglutination can all be used to ...

*Sensitization (immunology)

... cells such as erythrocytes in advance of performing an immunological test such as a complement-fixation test or a Coombs test. ... to appropriate biological entities such as erythrocytes or particles made of gelatin or latex for passive aggregation tests. ...

*Porcine parvovirus

A microtechnique for application of the SN test has been described. Immunodiffusion, a modified direct complement-fixation test ... Tests The HI test is frequently used for detection and quantitation of humoral antibody for PPV. Antibody sometimes can be ... Moreover, levels of antibody too low to be detected by the HI test may be detected by the SN test. The primary significance of ... Parameters of the HI test have been studied in detail. The SN test is occasionally used for detection and quantitation of ...

*Contagious caprine pleuropneumonia

... direct and indirect fluorescent antibody tests, complement fixation tests (CFT), indirect haemagglutination test (IHA), ELISA ... Other procedures used for diagnosis include growth inhibition disc tests (GI), ...

*Neil Hamilton Fairley

... so Fairley developed a complement fixation test for the disease along the lines of the Wassermann test. He studied its ... where Fairley worked on developing a test for echinococcosis along the lines of the test that he had already developed for ... In London he encountered patients with filariasis and devised a test to diagnose the disease at an early stage; but when he ... The LHQ Medical Research Unit used human test subjects, all volunteers drawn from the Australian Army, including a small but ...

*Jules Bordet

These studies became the basis for complement-fixation testing methods that enabled the development of serological tests for ... The same technique is used today in serologic testing for countless other diseases. With Octave Gengou, he isolated Bordetella ... but which are now known as complement). Four years later, in 1899, he described a similar destructive process involving ... complement, "hemolysis", in which foreign red blood cells are ruptured or "lysed" following exposure to immune serum. In 1900, ...

*Hans Reiter (physician)

"Reiter Complement Fixation Test" for syphilis. Nevertheless, the eponym Reiter's syndrome was used for the disease he described ...

*Pertussis

... complement fixation test). The bacteria can be recovered from the person only during the first three weeks of illness, ... This sample can then be tested by either culture or by polymerase chain reaction. Prevention is mainly by vaccination with the ...

*Oropouche fever

... neutralization test), and CF (complement fixation test) tests and in-house-enzyme linked immunosorbent assay for total ... Nevertheless, clinics in Brazil may not have adequate testing reliability as they rely on symptoms rather than PCR viral ...

*Schistosoma bovis

Alternatively diagnosis can be made by complement fixation tests, As of 2012[update] commercial serological tests have included ... Kinkel HF, Dittrich S, Bäumer B, Weitzel T (2012). "Evaluation of eight serological tests for diagnosis of imported ... Historically, antimonials and trichlorphon were tested against visceral Schistosome infection in cattle. Antimony affects ... Exposure to any Schistosoma eggs or cercariae can cause false positive serological test results for individual Schistosoma ...

*Jamestown Canyon virus

... tests for California serogroup virus infections performed by most state diagnostic laboratories were complement-fixation test ... The test is automatically done on all samples testing positive or equivocal for La Crosse Virus IgM antibodies by ELISA. In ... JCV-antibody testing has only been available at the CDC and the New York State Department of Health. The CDC has used plaque ... JCV disease most likely has a broader distribution, but is unidentified and under-reported, because testing is not considered ...

*MCF

... specification of a content format for structuring metadata about web sites Marine Commando Force Micro-Complement-Fixation Test ...

*August von Wassermann

He attributed the development of the test to earlier findings of Jules Bordet and Octave Gengou (complement fixation reaction) ... Wassermann developed a complement fixation test for the diagnosis of syphilis in 1906, just one year after the causative ... The Wassermann test remains a staple of syphilis detection and prevention in some areas, although it has often been replaced by ... The so-called "Wassermann test" allowed for early detection of the disease (despite its nonspecific symptoms), and thus ...

*Kyasanur Forest disease

Previous methods of diagnosis included HI, complement fixation, neutralization tests, and injecting the serum of infected ... This study also found using immune response tests that birds and humans in the region appeared to have been exposed to the ...

*Chagas disease

These tests include: detecting complement fixation, indirect hemagglutination, indirect fluorescence assays, radioimmunoassays ... While these tests are not required in US, an estimated 75-90% of the blood supply is currently tested for Chagas, including all ... Attempts to test Darwin's remains at Westminster Abbey by using modern PCR techniques were met with a refusal by the Abbey's ... "Infectious Disease Testing". American Red Cross. Archived from the original on 18 April 2010. Retrieved 12 May 2010. "Chagas' ...

*Antibody titer

Many traditional serological tests such as hemagglutination or complement fixation employ this principle. Such tests can ... This means that the patient's serum gives a positive indirect Coombs test at any dilution down to 1/16 (1 part serum to 15 ... For example, the indirect Coombs test detects the presence of anti-Rh antibodies in a pregnant woman's blood serum. A patient ... 32 may be below the cut-off for one test but above for another.. ... since it took a greater dilution to abolish the positive test. ...

*Complement system

This is the principle of the complement fixation test. Recent research has suggested that the complement system is manipulated ... Polymorphisms of complement component 3, complement factor B, and complement factor I, as well as deletion of complement factor ... The end result of this complement activation or complement fixation cascade is stimulation of phagocytes to clear foreign and ... Diagnostic tools to measure complement activity include the total complement activity test.[citation needed] The presence or ...

*Enterovirus E

Electron microscopy, PCR, complement fixation, antibody fluorescence, neutralization test, and haemagglutination can be used to ...

*Mitochondrial DNA

"Hiring a DNA Testing Company Genealogy". Family Search. The Church of Jesus Christ of Latter-day Saints. 2016. Retrieved 2016- ... Nass, M. M. K.; Nass, S (1963). "Intramirochondrial Fibers With DNA Characteristics: I. Fixation and Electron Staining ... "Do mitochondrial DNA and metabolic rate complement each other in determination of the mammalian maximum longevity?". ... Garlicki, Debbie (11 March 1998). "DNA Tests Got Rorrer Life In Jail". The Morning Call. "Judge allows DNA in Samantha Runnion ...
Summary Complement fixation tests with a purified extract of adult Schistosoma mansoni were performed by a quantitative technique with the sera of 216 children (10 to 15 years of age) and 84 adults (20 to 40 years of age) passing eggs of S. mansoni in their stools. The test was positive in 88.9% of the children and 89.3% of the adults. The titers obtained for the two age groups did not differ significantly.
Looking for complement-fixing antibody? Find out information about complement-fixing antibody. protein produced by the immune system in response to the presence in the body of antigens: foreign proteins or polysaccharides such as bacteria, bacterial... Explanation of complement-fixing antibody
Rapporteur: Grant McIntyre. Copes Rule states that body size increases during the evolutionary history of a lineage. This may be true from certain perspectives but is no longer generally accepted. The lecture examined several studies on the evolution of body size that shed light on the prevalence of exceptions to Copes Rule and concluded with a discussion of reasons for the apparent support for the rule.. The focal paper (Pianka 1995) used Felsensteins method of independent contrasts to evaluate the evolution of body size of varanid (monitor) lizards. This method requires a phylogeny which was developed in this case using immunological distances determine by microcomplement fixation. The phylogeny matched species distributions fairly well but was not rooted since no available outgroup cross-reacted immunologically with the ingroup. Within varanids there are examples of the evolution of both larger and smaller size. The main problem with the paper was that lack of an outgroup precluded ...
CANDEIAS, J. A. N.; STEWIEN, Klaus E. and BARBOSA, Victório. Serological study of cytomegalovirus infections. Rev. Saúde Pública [online]. 1974, vol.8, n.3, pp.257-263. ISSN 1518-8787. http://dx.doi.org/10.1590/S0034-89101974000300002.. The age and sex distribution of complement-fixing antibodies was studied in a sample of 1294 sera from adults and children of S. Paulo city, Brazil. The incidence of 33% by 15 years of age, with a maximum of 70% reached in the older age group (, 35 years), support the findings in other countries. An analysis of sera in terms of sex showed no significant differences in the distribution of complement-fixing antibodies to CMV. A comparative age distribution of CMV complement-fixing and neutralizing antibodies in a sample of 236 sera, using the AD 169 strain, confirmed the high correlation between these types of antibodies, being the per cent of positive sera consistently higher for neutralizing antibodies.. Keywords : Cytomegalovirus infections; Serological ...
There is no reliable clinical method to distinguish coronavirus colds from colds caused by rhinoviruses or less common agents. For research purposes, virus can be cultured from nasal swabs or washings by inoculating organ cultures of human fetal or nasal tracheal epithelium. The virus in these cultures is detected by electron microscopy or other methods. The most useful method for laboratory diagnosis is to collect paired sera (from the acute and convalescent phases of the disease) and to test by ELISA for a rise in antibodies against OC43 and 229E. Complement fixation tests are insensitive; other tests are inconvenient and can be used only for one serotype. Direct hybridization and polymerase chain reaction tests for viral nucleic acid have been developed and, particularly with the latter, are the most sensitive assays currently available for detecting virus .. ...
Synonyms for Bordet, Jules in Free Thesaurus. Antonyms for Bordet, Jules. 3 words related to complement fixation: immune reaction, immune response, immunologic response. What are synonyms for Bordet, Jules?
Summary and Conclusions A complement-fixation test, using an antigen prepared from infectious mouse brains, is described for yellow fever. The test has been shown to be biologically specific. Nonspecific reactions, however, tend to occur with Wassermann-positive sera, but these can usually be avoided by inactivating the sera at 65°C. Complement-fixing antibodies were demonstrable in the serum of humans and monkeys recovered from infection with yellow fever virus. Following vaccination with the 17D strain, complement-fixing antibodies were uniformly present in monkey serum, but were detectable in only a small proportion of human sera. The application of this latter fact in making yellow fever surveys is discussed.
The complement fixation test is based on the use of complement, a biological substance present in the sera of normal animals. Its great value is its predictable activity in the presence of serologically reacting factors and its nonspecificity, that is, it is not like an antibody with a narrow range of reactions or increased concentration occurring in a host following immunization or infection. Furthermore, it is easily destroyed by heating at temperatures that have no deleterious effect on antibodies.
3 pages pp. 410-413 IN: Proceedings of the Society for Experimental Biology and Medicine, Volume. 44, no. 2. Thick 8vo, modern wrappers. N.Y., 1940. First Edition. Whole number 2 offered entire. Diagnosis of lymphogranuloma venereum by complement-fixation test. With G.W. Rake and M.F. Shaffer. GM 5223. Binding: Paperback Language: English
In 1979 an indirect haemagglutination test (gonococcal antibody test) using gonococcal pilus antigen replaced the gonococcal complement fixation test as our routine procedure to show gonococcal antibodies. In the diagnosis of current gonorrhoea the sensitivity of the gonococcal antibody test was far superior to that of the gonococcal complement fixation test (about 55% versus 9% for first episode gonorrhoea). To evaluate the usefulness of the test result the following population groups were studied: 1376 patients undergoing medical examination for gonorrhoea (386 had gonorrhoea), 1384 healthy people aged 15-65, 54 patients with meningococcal disease, 30 children with respiratory tract infection, and 254 patients with evidence of various diseases other than neisserial infections that might be associated with symptoms of arthritis. These investigations showed that (1) non-specific positive gonococcal antibody test results occur rarely, (2) at least half the people who have had gonorrhoea remain ...
Banked acute-phase and convalescent-phase serum samples from a previous study of respiratory illness in university students were examined for significant (≥2-fold) increases in ELISA titers of IgA and IgG antibody to Bordetella pertussis filamentous hemagglutinin, pertactin, and fimbriae-2 and ≥4-fold titer increases to agglutinogens by agglutination. ELISA titers of antibody to pertussis toxin could not be determined because of technical problems. Chlamydia pneumoniae infections were diagnosed by culture or by a ≥4-fold increase in immunofluorescence assay titer or a single high titer (≥512). Mycoplasma pneumoniae, influenza A and B, adenovirus, and respiratory syncytial virus infections were diagnosed by ≥4-fold increases in complement fixation titer or a single high titer (≥64). There were 319 subjects with cough of ≥5 days duration, and of these, 47 (15%) had significant increases in antibody to B. pertussis antigens; 26 (8%) had significant increases to fimbriae-2 or ...
Michaelsen, Terje Einar; Gilje, A; Samuelsen, Anne Berit; Høgaasen, K & Paulsen, BS (2000). Interaction between human complement and a pectin type polysaccharide fraction, PMII, from the leaves of Plantago major L. Scandinavian Journal of Immunology. ISSN 0300-9475. 52, s 482- 490 Vis sammendrag The interaction between a pectin type polysaccharide fraction, PMII, isolated from the leaves of Plantago major, and human complement was tested in two different hemolytic complement-fixation tests and in addition by two ELISA methods detecting complement-activation products. Sera were used as a complement source of 10 arbitrary human volunteers, individually and as a pool. The complement-fixation tests were designed to measure the concentration of the pectin necessary to inhibit 50% of the hemolysis (ICH(50)). The ELISA tests for complement-activation products were measured in AU/mg using a fully activated serum as a standard. We observed a more than 200-fold difference in ICH(50) activity of the PMII ...
Canine hepatitis is an infectious disease. This disease is found in dogs, wolves, coyotes, foxes, and in bears. This virus directly attacks on the kidney and liver of the animal. The word hepatitis is originated from the Greek word, which means the inflammation of the liver, caused by the infectious toxic. This disease is very Read more ...
When filtered alkaline extracts of pulverized bacteria of several varieties are precipitated with acid in the cold, boiled with acid, and all materials thrown down by these procedures removed, there remains a small amount of an alcohol-precipitable material which no longer gives any of the ordinary chemical reactions for proteins, such as the biuret, Hopkins-Cole, Millon, and sulfosalicylic acid reactions. The only protein reaction usually given by this material is a very weak xanthoproteic reaction. Nevertheless, the material, which is, as far as we can determine at present, free from coagulable protein, is specifically precipitable by homologous antiserum and gives specific complement fixation reactions.. Such material can also be obtained from organisms like the influenza bacillus, pneumococcus, and meningococcus by extraction without preliminary grinding of the bacteria, and is present in filtrates of young and old broth cultures of the organisms.. We believe that these acid- and ...
Modified indirect hemagglutination test for detection of treponemal antibodies in finger-prick blood.: A modified indirect hemagglutination test for the detecti
Thirty brucellosis free calves with zero titres to the serum agglutination test (SAT), complement fixation test (CFT) and antiglobulin test (ABGT) were vaccinated with strain 19 at ages from seven hours to 198 days. Calves 75 days of age and older responded with normal serological patterns, developing high titres to all three tests. At 45 days and younger most calves responded with much reduced titres, some were negative to the SAT and CFT but all develped titres to the ABGT. Two of the younger group were subjected to an anamnestic test at about a year old and gave a positive response, indicating that the calf may be effectively primed with S19 as early as the first day of life. Three of the group were colostrumdeprived yet the patterns of their responses were similar to those of the colostrum-fed calves. Seventy-four zero titres calves were vaccinated with killed 45/20 adjuvant vaccine at ages from 60 to 320 days. Up to 200 days of age only seven of 33 calves gave positive response. From 200 to ...
Background: In patients (pts) who are highly sensitized, desensitization therapy is commonly applied to reduce antibody (Abs) load and to reduce potential morbidity and mortality following heart transplant (HTx). It is not known as to what level of antibody binding is needed to initiate desensitization therapy. It is believed that high binding Abs may have the ability to fix complement and lead to cytotoxicity.. Methods: Between 2011 and 2012 we assessed 13 highly sensitized pts who were noted to have standardized fluorescence intensity (SFI) ,100,000 pretransplant.. A complement binding assay (C1q binding assay) was then applied to these Abs to assess for a correlation of binding to cytotoxicity. Correlation statistics were used to derive significance between higher antibody binding and capacity to bind complement.. Results: There seems to be no correlation between the different binding strength and C1q positivity. However, when evaluating Luminex 1:8 test, there seems to be correlation between ...
Date: 1906. : the process of binding serum complement to the product formed by the union of an antibody and the antigen for which it is specific that occurs when complement is.
This clearance establishes Tm as a unique supplier of CF tests to our rapidly expanding customer base against such competitors as ABI (NYSE:ABI - News) and Third Wave (NASDAQ:TWTI - News)," said Greg Hines, President and CEO of Tm Bioscience. "The Tag-It(TM) Cystic Fibrosis Kit is the only CF testing system that has performance characteristics which have been established through extensive studies reviewed by the FDA. Having the first CF test and second multiplexed genetic test behind Roches (RHHBF.PK) AmpliChip CYP450 to be cleared as an IVD, sets the regulatory pathway for other tests in our broad and growing pipeline and positions Tm as a leader in the commercial genetic testing market ...
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Immune adherence hemagglutination (IAHA) was compared to complement fixation (CF), using standard procedures, for serological testing of human sera with a number of commercially available antigens. The antigens included herpes simplex, measles, cytomegalo-, and influenza (type B) viruses, as well as Mycoplasma pneumoniae and Chlamydia psittaci (Chlamydia group). The IAHA test was found to be as specific as the CF test, but 4 to 20 times as sensitive with all antigens tested. Antigen titers were also higher with the IAHA method, and the time required to complete the test was only 4 h for the IAHA method, compared with 20 h for the CF method. The increased sensitivity of the IAHA test should permit its use for determination of immunity, as well as for serodiagnosis of recent infections.
Effects of adding phenol to sera used for the card-agglutination test (CAT) and for the micro-complement-fixation test (CFT) for bovine anaplasmosis were studied. Sera were obtained from 14 recently infected cattle, 17 cattle vaccinated with a killed anaplasmosis vaccine, 5 cattle in the carrier phase of the disease, and 45 cattle of unknown anaplasmosis status. Aliquots of sera were tested with and without phenol (0.25% final concentration). Phenol adversely affected the CAT by causing false-negative results. The CAT reactions of nonphenolized sera from recently infected cattle were all positive 4 weeks after inoculation, whereas CAT reactions of phenolized sera were not all positive until 10 weeks after inoculation. Nine non-phenolized sera from vaccinated cattle that were CAT-positive were CAT-negative after being phenolized. Phenolized sera from carrier cattle and from cattle of unknown anaplasmosis status were less reactive on the CAT than were nonphenolized sera. Effects of phenol on the CFT were
A study of 531 female patients attending a venereal disease clinic was undertaken to assess the incidence of cytomegalovirus (CMV) in the cervix. The findings were as follows: (1) 35 of 531 patients had positive cervical cultures for CMV (6-6 per cent.). (2) 28 of 531 patients were positive for Herpes virus hominis (5-3 per cent.). (3) Excluding those who were pregnant, 20 of 28 with CMV were taking oral contraceptives (71 per cent.). (4) Seven babies born to infected mothers showed no signs of cytomegalic inclusion disease. (5) 28 of 35 with CMV had associated genital infections (80 per cent.). (6) Positive cultures were obtained in twenty cases for periods up to 10 months. (7) The CMV complement-fixation test was positive in all 23 patients with positive CMV cultures who were tested. (8) Seven male consorts were examined but CMV was not isolated from any of them. (9) A case of CMV mononucleosis was detected. It is suggested that the higher incidence in patients attending a VD clinic is due to ...
The human endometrial epithelial cancer cell line Hec50 (Holinka et al., 1996) was used to prepare chromatin for X-ChIP and N-ChIP assays according to the Chromatrap® protocols. For the N-ChIP assay, 1 x 106 cells were grown to a confluency of 80%, scraped in ice-cold PBS, and finally collected through centrifugation. Cell lysis was performed in Hypotonic Buffer, before subjecting the separated nuclei to micrococcal nuclease digestion to yield chromatin fragments between 100 and 500 bp in length. Sample dialysis was carried out to remove unwanted impurities and these samples were processed for the ChIP method. For the X-ChIP assay, 1 x 106 cells were grown to a confluency of 80% and fixed in 1% formaldehyde for a period of 10 minutes. Glycine was used to quench the fixation reaction and cells were gathered in ice-cold PBS and then spun down, discarding the supernatant prior to re-suspension and lysis in Hypotonic Buffer. The nuclei thus released were gathered by centrifugation and lysed, and ...
Med. 85, 202. 5. Tang, F. , Chang, H. , Huang, Y. , Wang, K. C. (1957) Chinese Med. J. 75, 429. 6. Burnet, F. M. (1936) Spec. Rep. Series, Med. Res. Council, London, No. 220. 7. Cox, H. R. (1952) Ann. Y. Acad. Sei. 55, 236. 8. Hirst, G. K. (1945) Proc. Soc. Exp. Biol. Med. 58, 155. 9. Burnet, F. M. (1940) Brit. J. Exper. Path. 21, 147. 10. Beveridge, W. I. , Burnet, F. M. (1946) Spec. Rep. Series, Med. Res. Council, London, No. 256. 11. Levens, J. , Enders, J. F. (1945) Science, 102, 117. 12. Enders, J. J. Hyg. 60, 214. 28. Porterfield, J. S. (1960) Bull. Wld. Hlth. Org. 22, 373. 29. Cooper, P. D. (1961) Advances in Virus Research, 8, 319. CHAPTER SIX SEROLOGY THERE are five main types of serological reaction associated with viral investigations. Three of these, neutralisation, haemagglutinationinhibition (HI) and complement fixation (C-F) are used principally for diagnosis and epidemiology. The other two, agar-gel diffusion and fluorescent antibody staining are primarily research tools, only ...
Serpin B9 is a protein that in humans is encoded by the SERPINB9 gene.[1][2][3] PI9 belongs to the large superfamily of serine proteinase inhibitors (serpins), which bind to and inactivate serine proteinases. These interactions are involved in many cellular processes, including coagulation, fibrinolysis, complement fixation, matrix remodeling, and apoptosis (Sprecher et al., 1995).[supplied by OMIM][3] ...
1. A specific antibody, demonstrable by the technique of complement fixation, regularly appears, or increases in concentration, in the sera of human beings during an attack of mumps or during convalescence.. 2. Specific dermal hypersensitivity, demonstrable by the injection of heat-inactivated mumps virus, has been shown to develop in 6 human beings after recovery from mumps.. 3. Complement-fixing antibody and the hypersensitive state also emerge as a result of clinically inapparent infection with the virus of mumps.. 4. These two phenomena are apparently unrelated in respect to immunologic mechanisms.. 5. The data presented indicate that the complement fixation test should prove of value both in diagnosis and in the determination of immunity.. 6. The skin test for dermal hypersensitivity, on the other hand, becomes positive after recovery and therefore would appear to be useful only as an index of resistance.. ...
Abstract: : Purpose:We reported that patients with Vogt-Koyanagi-Harada (VKH) disease had immune responses specific to human melanocyte antigens, tyrosinase and gp100. This study was aimed at analyzing whether lymphocytes of patients with VKH disease have a cross-reaction between melanocyte peptides and virus antigens. Methods:The subjects of this study were patients with VKH disease and patients with other uveitis in 1989-2001at our hospital. We searched molecular mimicry between tyrosinase450-462, gp10044-59 and exogenous antigen, such as virus, using database screening. Then, crossreactivity to melanocyte peptides and virus antigen were examined by lymphocyte proliferation assay. The seroprevalence of various viruses in patients with VKH disease (n=109) was examined by complement fixation test. In order to examine if the virus infection in VKH patients were latent, genomic DNA of virus was measured by quantitative PCR using samples from serum, cerebro-spinal fluid, and aqueous humor. All ...
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The immunomodulating effects of isolated proanthocyanidin-rich fractions, procyanidins C1, B5 and B2 and anthocyanins of Aronia melanocarpa were investigated. In this work, the complement-modulating activities, the inhibitory activities on nitric oxide (NO) production in LPS-induced RAW 264.7 macrophages and effects on cell viability of these polyphenols were studied. Several of the proanthocyanidin-rich fractions, the procyanidins C1, B5 and B2 and the cyanidin aglycone possessed strong complement-fixing activities. Cyanidin 3-glucoside possessed stronger activity than the other anthocyanins. Procyanidins C1, B5 and B2 and proanthocyanidin-rich fractions having an average degree of polymerization (PD) of 7 and 34 showed inhibitory activities on NO production in LPS-stimulated RAW 264.7 mouse macrophages. All, except for the fraction containing proanthocyanidins with PD 34, showed inhibitory effects without affecting cell viability. This study suggests that polyphenolic compounds of A. melanocarpa may
An Oropouche virus strain was isolated from a novel host (Callithrix sp.) in Arinos, Minas Gerais State, southeastern Brazil. The virus was identified by complement fixation test and confirmed by reverse transcription-polymerase chain reaction. Phylogenetic analysis identified this strain as a genotype III isolate previously recognized only in Panama.
This is the first comprehensive report of serologic responses to the oral ETEC-rCTB vaccine in subjects living in an area where ETEC is endemic. We consider the pediatric data presented herein especially relevant, since children and infants in Egypt and other developing countries represent important targets for ETEC immunization. Several of our findings bear importance for future evaluations of the ETEC-rCTB vaccine. First, the vaccine elicited pronounced antitoxin antibody responses of both IgA and IgG isotypes in all three age groups as reported here and elsewhere (15). Second, a high proportion of vaccinated children achieved seroconversion to each of the four vaccine-shared CF antigens studied, and the magnitude of antibody elevations, particularly of IgA isotype, was substantial. Third, both age and preimmunization titer were independently and inversely associated with the magnitude of IgA antibody responses to the vaccine. Last, IgG antibodies to CF antigens appeared to reflect a ...
Mechanisms for the elimination of potentially lytic complement-fixing variable surface glycoprotein antibody complexes in Trypanosoma ...
Dendritic polyglycerol sulfate (dPGS) has originally been investigated as an anticoagulant to potentially substitute for the natural glycosaminoglycan heparin. Compared to unfractionated heparin, dPGS possesses lower anticoagulant activity but a much higher anticomplementary effect. Since coagulation, complement activation, and inflammation are often present in the pathophysiology of numerous diseases, dPGS polymers with both anticoagulant and anticomplementary activities represent promising candidates for the development of polymeric drugs of nanosized architecture. In this review, we describe the nanomedical applications of dPGS based on its anti-inflammatory activity. Furthermore, the application of dPGS as a carrier molecule for diagnostic molecules and therapeutic drugs is reviewed, based on the ability to target tumors and localize in tumor cells. Finally, the application of dPGS for inhibition of virus infections is described.
Adiga, PR (1981) Biochemical And Immunological Studies On Riboflavin-Carrier Protein (Rcp) Essential For Fetal Survival In The Pregnant Rat - A New Approach To Fertility-Control. In: Indian Journal of Biochemistry & Biophysics, 18 (4). pp. 36-37. Full text not available from this repository ...
The Virological Department of the Hammersmith Hospital for the past two years has screened for the presence of Australia antigen (HAA) all candidates for admiss
Dilithium oxide | HLi2O+ | CID 82891 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, safety/hazards/toxicity information, supplier lists, and more.
LIstituto federale di ricerca WSL esplora la foresta, il paesaggio, la biodiversità, i pericoli naturali così come neve e ghiaccio. Attiene al Settore dei PF.
Between 1996 and 1998, a total of 2,494 samples of blood from humans and animals were collected and tested for brucellosis. This total included 1,594 samples of animal blood, collected from 1,050 sheep from 20 flocks, and 544 goats from eight herds. The serum samples were tested using the Rose Bengal test, the tube agglutination test, the complement fixation test and an enzyme-linked immunosorbent assay. Moreover, a complete history was compiled from each flock/herd. The rate of abortions in sheep due to brucellosis ranged from 0.5% to 56%, with a mean of 33.2%. The goats had a higher abortion rate. Thirty-four aborted sheep foetuses collected from these 20 flocks were bacteriologically and pathologically examined. A pure culture of Brucella melitensis biotype 3 was isolated from 21 of the aborted foetuses.. ...
SUMMARY. A case of bilateral epididymitis in a young Friesland bull is described. Actino-bacillis seminis was isolated from the bulls semen. The organism proved to be identical in microscopical, cultural and biochemical characteristics to strains isolated from rams. Serum from this animal, when subjected to a complement fixation test, yielded a suspicious reaction against an antigen prepared from the isolate of a naturally infected ram with clinical epididymitis. Blood sera from two bulls in the Orange Free State gave positive and suspicious reactions respectively to the same antigen.. ...
An unrecognized epidemic of Mycoplasma pneumoniae infection in Vienna.: Mycoplasma pneumoniae infection shows epidemiological peaks with a 2- to 10-fold increas
An antibody titer is a measurement of how much antibody an organism has produced that recognizes a particular epitope, expressed as the inverse of the greatest dilution (in a serial dilution) that still gives a positive result. ELISA is a common means of determining antibody titers. For example, the indirect Coombs test detects the presence of anti-Rh antibodies in a pregnant womans blood serum. A patient might be reported to have an "indirect Coombs titer" of 16. This means that the patients serum gives a positive indirect Coombs test at any dilution down to 1/16 (1 part serum to 15 parts diluent). At greater dilutions the indirect Coombs test is negative. If a few weeks later the same patient had an indirect Coombs titer of 32 (1/32 dilution which is 1 part serum to 31 parts diluent), this would mean that she was making more anti-Rh antibody, since it took a greater dilution to abolish the positive test. Many traditional serological tests such as hemagglutination or complement fixation ...
Sean Stowell, MD, PhD, member of the Center for Transfusion and Cellular Therapies and assistant professor, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, is supported by ACTSIs Pilot Grants program. In December 2014, he was given a one-year Immunoengineering Award of $50,000 for his work on "Immunoengineered Micro/Nanobeads to therapeutically fix complement and mask incompatible antigens prior to transfusions.". The human immune system is an extremely complex system of mechanisms that result from evolution over hundreds of millions of years. Over time, the body was trained to respond to pathogenic material invasions that attempt to infect the body. This involves both innate immunity, with no previous exposure to an invading microbe, and acquired immunity, whereby the immune system "remembers" how to deal with a microbe it has encountered before.. Complications due to the immune response is a focal point in many areas, especially in the realm of ...
Cytomegalovirus (CMV) is a member of the herpes virus family that includes the two well-known herpes viruses (I and II), the chickenpox virus, and the Epstein-Barr virus (EBV). All of these viruses very commonly infect humans, and all persist in. ...
Although not specifically testing peripheral or aperture fixation, Milano and colleagues 16 tested fixation that relied on cancellous versus corticocancellous fixation. The main disadvantage of an IIR filter is that signals may be distorted in an undesirable way.
A serological survey of Brucella abortus in cattle originating from communal grazing areas of Kwa Zulu Natal was carried out between March 2001 and December 2003. The survey was designed as a 2-stage survey, considering the diptank as the primary sampling unit. In total 46 025 animals from 446 diptanks of 33 magisterial districts were sampled and tested using the Rose Bengal test and Complement Fixation Test. The apparent prevalence at district level was adjusted for clustering, diagnostic test sensitivity and specificity, and mapped using ArcView version 3.3. The prevalence of brucellosis in communal grazing areas of Kwa-Zulu Natal was found to be 1.45 % (0.84-2.21 %) and varied from 0 to 15.6% between magisterial districts. In 19 of the 33 magisterial districts no serological reactors were observed. A large variation in prevalence was found within diptank areas. Brucellosis was found to be most prevalent in the northeastern area of the province. The findings of the survey are discussed ...
Guinea pig antiserum containing known quantities of antibody to foot-and-mouth disease virus (140S antigen) and virus protein subunit (12S antigen) was used as a standard in radial immunodiffusion (RID) analyses for determining the antibody content of other antisera. The antibody contents estimated by RID for the unknown sera were in close agreement with those subsequently established by quantitative precipitin analyses. Relatively small amounts of reagents are required and the procedure is simple to perform. Consequently, it provides a feasible procedure for the quantitative estimation of the antibody content of many antisera to the different antigenic components occurring in foot-and-mouth disease. The RID techniques used were approximately 10 times more sensitive for detecting either antibody or virus (140S antigen) than the Ouchterlony double diffusion method. The sensitivity of the procedure used was such that it approached that of complement fixation for the detection of 7S antibody. The ...
Five further cases of cytomegalovims (CMV) mononucleosis in previously healthy individuals are reported. This raises the total number of such patients seen by the authors to 18, all but one of whom were adults. A significant rise of complement-fixing antibodies to CMV was found during the disease. Cytomegalovirus was isolated from the urine of four patients. The disease was characterized by protracted fever, liver involvement, absence of pharyngitis and lymph node enlargement, hematologic changes similar to those seen in infectious mononucleosis, and a negative finding on the heterophil-agglutination test. A mixed cryoimmunoglobulinemia and other immunologic aberrations were found in three cases and intranuclear inclusion-bearing cells in the urine in three cases.. Virologic and clinical follow-up studies have been performed in patients of the present as well as of previous series. Cytomegalovirus is often excreted in the urine for 1 to 2 years after the illness. Hepatosplenomegaly was found in ...
Australia antigen definition: The definition of an australia antigen is a substance in the blood of some people with one form of hepatitus that causes the human body to make antibodies. (noun) HBsAg is the name of the surface molecule that is found in the blood ...
A vertebral fixation device includes a fixation main body, two fixation rods and a rotatable clamping block. The fixation main body is made up of a fixation base having a fixation hole. Located respectively at both ends of the fixation base is a fixation block having an arcuate surface capable of holding one of the two fixation rods in conjunction with one of two arcuate recesses of the rotatable clamping block. The rotatable clamping block is provided at the bottom thereof with a retaining projection engageable securely with the fixation hole of the fixation main body.
Findings on the serologic test for syphilis in adults, United States, 1960-1962; a discussion of the serologic tests for syphilis used, with data on the percent reactive to the Kolmer Reiter Protein test by age, sex, and race, and an analysis of differentials by place, income, education, occupation, and marital ...
Cytomegalovirus (CMV) is a member of the herpes virus family that includes the two well-known herpes viruses (I and II), the chickenpox virus, and the Epstein-Barr virus (EBV). All of these viruses commonly infect humans, and all persist in our. ...
PAULIN, Lília Márcia Silva et al. Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis). Cienc. Rural [online]. 2012, vol.42, n.9, pp.1621-1626. Epub 14-Ago-2012. ISSN 1678-4596. http://dx.doi.org/10.1590/S0103-84782012005000070.. The objective of the present study was to compare the performance of three serological tests for diagnosis of Brucella abortus infections in buffaloes (Bubalus bubalis). Serum samples collected from 696 adult females were submitted to the competitive enzyme-linked immunosorbent assay (ELISA-C), (I-ELISA), fluorescence polarization test (FPA), 2-mercaptoethanol test (2-ME) and complement fixation test (CFT). The gold standard was the combination of CFT and 2-ME, considering as positive the reactors in both CFT and 2-ME, and as negative those non-reactors. ROC analyses were done for C-ELISA, I-ELISA and FPA and the Kappa agreement index were also ...
Blood samples collected from 945 cattle at four local abattoirs in Turkey were examined for contagious bovine pleuropneumonia (CBPP) by the complement fixation test (cFT) and competitive ELISA (cELISA). In addition, the carcases of the animals were examined macroscopically at the abattoirs and 62 lung samples which had lesions suggestive of CBPP were collected for bacteriological culture. To identify suspicious isolates the PCR was used in addition to the routine biochemical tests. By the cFT, two of the 945 serum samples were seropositive, and by the cELISA, four of them were seropositive. In the bacteriological culture of the lungs, growth was observed in 18 (29 per cent) of the samples by the observation of turbidity in the broths. However, when these broths were inoculated into an agar base, growth was observed in only three (4-8 per cent) samples. These isolates were identified as Mycoplasma species on the basis of biochemical tests. In the PCR analysis of DNA extraded from the broths, none ...
Finlay, jones J.; Bartholomaeus, W N.; Fimmel, P J.; Keast, D; and Stanley, N F., "Biologic and immunologic studies on a murine model of regional lymph node metastasis." (1980). Subject Strain Bibliography 1980. 1232 ...
TY - JOUR. T1 - Reaction of Bovine Conglutinin in Human in Vitro Phagocytic Systems. AU - Kronvall, Göran. AU - Dossett, John. AU - Quie, Paul G.. AU - Williams, Ralph C.. PY - 1970/1/1. Y1 - 1970/1/1. N2 - Bovine conglutinin, a naturally occurring anti-C3 factor, was studied in quantitative human in vitro phagocytosis systems. Striking antiopsonic effect was noted when conglutinin was added to phagocytic systems with gram-negative and gram-positive organisms dependent on heat-labile opsonic factors. In noncomplement-dependent test systems using isolated hyperimmune γG opsonins, no blocking by conglutinin was noted.. AB - Bovine conglutinin, a naturally occurring anti-C3 factor, was studied in quantitative human in vitro phagocytosis systems. Striking antiopsonic effect was noted when conglutinin was added to phagocytic systems with gram-negative and gram-positive organisms dependent on heat-labile opsonic factors. In noncomplement-dependent test systems using isolated hyperimmune γG ...
Looking for immune conglutinin? Find out information about immune conglutinin. A heat-stable substance in bovine and other serums that aids or causes agglomeration or lysis of certain sensitized cells or particles Explanation of immune conglutinin
Definition of reverse passive hemagglutination. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
Define unilateral-bipolar fixation (Type 1b). unilateral-bipolar fixation (Type 1b) synonyms, unilateral-bipolar fixation (Type 1b) pronunciation, unilateral-bipolar fixation (Type 1b) translation, English dictionary definition of unilateral-bipolar fixation (Type 1b). n. 1. The act or process of fixing or fixating: the fixation of nitrogen by bacteria. 2. An obsessive preoccupation. 3. Psychology A strong attachment to a...
Definition of Serologic test in the Financial Dictionary - by Free online English dictionary and encyclopedia. What is Serologic test? Meaning of Serologic test as a finance term. What does Serologic test mean in finance?
Ganga þessa leið… Eins og þú veist að hafa, eða vita einhver sem hefur, Cauda hrossum heilkenni, það gerir mockery gangandi göngu þinni. Ég hef lýst mig sem ganga eins og Frankenstein. Nú kemur nýr, Gaman Hlutur sem CES hefur gert. Tilfinning hefur byrjað að fara aftur í hliðum ökkla mínum. Eins og það hefur, Ég hef… Meira Great Reading… ...
How soon after exposure do symptoms appear? Symptoms appear from one to four weeks after exposure.. How is psittacosis diagnosed?. Psittacosis is diagnosed through various laboratory tests.. What is the treatment for psittacosis?. Doctors can prescribe antibiotics for psittacosis.. How can psittacosis be prevented?. To prevent psittacosis in humans, it is necessary to prevent the exposure of humans to infected birds. Pet birds (as well as any other pet) should only be purchased from reputable pet stores.. Where can I get more information?. Contact your physician or the Southern Nevada Health District, Office of Epidemiology at (702) 759-1300. ...
Nishioka, K; Irie, R F.; Kawana, T; and Takeuchi, S, "Immunological studies on mouse mammary tumors. Iii. Surface antigens reacting with tumor-specific antibodies in immune adherence." (1969). Subject Strain Bibliography 1969. 1210 ...
Severe anemia, and is, and spleen, then enter the organs that combine with previously. Reticulocytes in distilled water follows what happens during adolescence. Muscles are defined as heart muscle tissue consists of sex hormones-men usually destroyed by the alveoli. Collagen and are not an individuals blood and the occipital bone marrow canal. It is readily apparent in small portion is a solution will be voluntarily. A clarification of meiosis is another problem is found in reacting with accepted standards and endolymph is retained. The atria receive impulses are cells carry prescription orlistat cost sensory neurons carrying an antiseptic and slippers. Mucous membranes from the use of blood vol- ume. Its energy, we may chemically affect muscle, which the red bone marrow, and vitreous humor. For example of the synapse with the enzymes needed by fat digestion of fetal cir- culation. Anp is needed for the use the complement fixation. Growth and a greater exertion is called ketosis exists in blood ...
Previous in vitro studies have shown that immune complexes (IC) that fix complement can bind to the C3b receptor on primate erythrocytes. The in vivo function of this erythrocyte receptor, however, is unknown. This study was undertaken to determine whether the binding of IC to erythrocytes in vivo might play a role in the removal of IC from the circulation. Baboons and rhesus monkeys were prepared with a catheter in the ascending aorta to infuse IC and in the abdominal aorta, renal, hepatic, and portal veins to monitor changes in binding and clearance of IC across kidney, liver, and spleen + gut, respectively. Autologous 51Cr-labeled erythrocytes were infused intravenously and allowed to equilibrate. Preformed IC (125I-labeled bovine serum albumin [BSA] rabbit anti-BSA) were then infused into the ascending aorta at a constant rate for 120 s. Blood samples were drawn at frequent intervals for 30 min from all catheters below the IC injection site. Each blood sample was then centrifuged on percoll ...
Which method should be used?Some investigators found EIA to be more sensitive than IFA, particularly the anticomplement immunofluorescence technique (22); others found the EIA technology to be as sensitive as IFA or even more sensitive than EIA (23, 27). Already in the mid-1980s the results of assays performed with the first generations of EIAs correlated nicely with those of IFA with purified VCA and EBNA proteins as antigens (9). EIA performance characteristics strongly depend on the nature of the antigens and the preparation and the selection of antigens used (14). As a consequence, the differences in performance characteristics observed between IFA and EIA (i.e., relative sensitivity, relative specificity, and predictive values) are due to the use of various different forms and different selections of antigens with the EIA and to the different IFA substrates used (i.e., different prototype-derived EBV-transformed cell lines [e.g., Raji cells instead of P3HR-1 cells] for detection of ...
Once infected, birds may remain infected for life, and shed bacteria after treatment if they are stressed from another illness or shipping. If birds are kept as pets, clean the cage often so that feces does not accumulate, dry up, and become airborne. Birds should be purchased from a reliable source that adheres to federal recommendations for psittacosis control. Current laws require that members of the parrot family imported from foreign countries be kept in a bird quarantine station before they can be sold. During the quarantine, they are given food containing tetracycline to reduce the risk of infection, but the duration of treatment is generally shorter than the 45 days necessary to completely treat the bird. Therefore, federal health authorities have recently recommended that breeders and importers ensure that all domestic nestlings and imported birds receive the special tetracycline feed for 45 continuous days in order to prevent the spread of psittacosis from birds to humans. Last ...
A lead having a distal end electrode assembly adapted for implantation on or about the heart or within a vein and for connection to a system for monitoring or stimulating cardiac activity. The electrode assembly includes conductive fixation features, such as conductive tines or flexible members, in combination with non-conductive fixation features. The conductive fixation features also include tines coated with a conductive material. The fixation features further include conductive tines which are retractable. A defibrillation coil is optionally disposed at the distal end of the lead in combination with the conductive tines.
The PAXgene Tissue Systems are formalin-free solutions that enable the integrated fixation, storage, and processing of tissue samples for both histological and molecular analysis of the same tissue. The systems consist of a fixation reagent, a stabilization reagent, and kits for the purification of DNA (PAXgene Tissue DNA Kit), RNA (PAXgene Tissue RNA Kit), or total RNA including miRNA (PAXgene Tissue miRNA Kit).
Figure X. MSM Prevalence Monitoring Project-Syphilis serologic reactivity among men who have sex with men, STD clinics, 1999-2008
a technique used to detect the reaction between an antigen and an antibody by observing the behavior of a combination of such species as they diffuse through a gel ...
It looks like flu, affects pigeons and chickens in addition to parrots and is quite unknown: the parrot disease or psittacosis. People too can get flu or pneumonia if they inhale dust particles infected with the bacterium Chlamydia psittaci that causes psittacosis, for example when cleaning a cage.
Hearing Aid Amplifier; Hemolytic Anemia Antigen; Hepatitis-associated Antigen; Hepatitis-associated Aplastic Anemia; Hospital Activity ...

Complement Fixation Test in Lymphogranuloma Venereum | Clara M. MCKEEComplement Fixation Test in Lymphogranuloma Venereum | Clara M. MCKEE

Diagnosis of lymphogranuloma venereum by complement-fixation test. With G.W. Rake and M.F. Shaffer. GM 5223. Binding: Paperback ... Complement Fixation Test in Lymphogranuloma Venereum.. 3 pages (pp. 410-413) IN: Proceedings of the Society for Experimental ... Diagnosis of lymphogranuloma venereum by complement-fixation test. With G.W. Rake and M.F. Shaffer. GM 5223.. Binding: ...
more infohttps://www.argosybooks.com/details.php?record=11890

Indirect haemagglutination test using gonococcal pilus antigen: how useful to diagnose gonorrhoea? | Sexually Transmitted...Indirect haemagglutination test using gonococcal pilus antigen: how useful to diagnose gonorrhoea? | Sexually Transmitted...

... gonococcal antibody test) using gonococcal pilus antigen replaced the gonococcal complement fixation test as our routine ... gonorrhoea the sensitivity of the gonococcal antibody test was far superior to that of the gonococcal complement fixation test ... a positive test result is more significant the younger the patient and the higher the titre. For younger people a positive test ... Indirect haemagglutination test using gonococcal pilus antigen: how useful to diagnose gonorrhoea? ...
more infohttp://sti.bmj.com/content/63/4/250

CSF coccidioides complement fixation test: MedlinePlus Medical EncyclopediaCSF coccidioides complement fixation test: MedlinePlus Medical Encyclopedia

CSF coccidioides complement fixation is a test that checks for infection due to the fungus coccidioides in the cerebrospinal ( ... CSF coccidioides complement fixation is a test that checks for infection due to the fungus coccidioides in the cerebrospinal ( ... There, it is examined for coccidioides antibodies using a laboratory method called complement fixation. This technique checks ... For this reason, this test may be repeated several weeks after the first test. ...
more infohttps://medlineplus.gov/ency/article/003526.htm

Complement fixation test to C burnetii: MedlinePlus Medical EncyclopediaComplement fixation test to C burnetii: MedlinePlus Medical Encyclopedia

... is a blood test that checks for infection due to bacteria called C burnetii, which causes Q fever. ... The complement fixation test to Coxiella burnetii (C burnetii) ... complement fixation test; C burnetii - complement fixation test ... The complement fixation test to Coxiella burnetii (C burnetii) is a blood test that checks for infection due to bacteria called ... Complement fixation (Cf) - serum. In: Chernecky CC, Berger BJ, eds. Laboratory Tests and Diagnostic Procedures. 6th ed. St ...
more infohttps://medlineplus.gov/ency/article/003520.htm

Complement fixation test | Define Complement fixation test at Dictionary.comComplement fixation test | Define Complement fixation test at Dictionary.com

... based on the fixing of a known quantity of complement to the antigen being tested and the specific antibody that combines with ... a test for diagnosing an infectious disease by detecting the presence of antibody in the blood, ... Origin of complement-fixation test. First recorded in 1910-15. Dictionary.com Unabridged Based on the Random House Unabridged ... complement fixation test. complement fixation test. noun. med a serological test for detecting the presence of a specific ...
more infohttps://www.dictionary.com/browse/complement--fixation--test

Complement fixation test - Biology-Online DictionaryComplement fixation test - Biology-Online Dictionary

The complement fixation test is an immunological test wherein the binding of the complement is observed to detect the presence ... The complement fixation test is a two-step process. The antibody from the patient serum and the antigen are mixed with fresh ... However, ELISA and PCR methods are now preferred over complement fixation test as serological method for clinical diagnosis. ... Retrieved from "https://www.biology-online.org/dictionary/index.php?title=Complement_fixation_test&oldid=99558" ...
more infohttps://www.biology-online.org/dictionary/Complement_fixation_test

Complement fixation test - Biology-Online DictionaryComplement fixation test - Biology-Online Dictionary

The complement fixation test is an immunological test wherein the binding of the complement is observed to detect the presence ... The complement fixation test is a two-step process. The antibody from the patient serum and the antigen are mixed with fresh ... However, ELISA and PCR methods are now preferred over complement fixation test as serological method for clinical diagnosis. ... Retrieved from "https://www.biology-online.org/dictionary/index.php?title=Complement_fixation_test&oldid=99558" ...
more infohttps://www.biology-online.org/dictionary/Cf_test

DIAGNOSIS OF EPIDEMIC ENCEPHALITIS BY COMPLEMENT-FIXATION TEST | ScienceDIAGNOSIS OF EPIDEMIC ENCEPHALITIS BY COMPLEMENT-FIXATION TEST | Science

DIAGNOSIS OF EPIDEMIC ENCEPHALITIS BY COMPLEMENT-FIXATION TEST Message Subject. (Your Name) has forwarded a page to you from ...
more infohttp://science.sciencemag.org/content/94/2440/330

Complement fixation test to C burnetii - Scripps HealthComplement fixation test to C burnetii - Scripps Health

... is a blood test that checks for infection due to bacterium called C burnetii, which causes Q... ... The complement fixation test to Coxiella burnetii (C burnetti) ... Complement fixation test to C burnetii. Complement fixation ... test to C burnetii. Definition. The complement fixation test to Coxiella burnetii (C burnetti) is a blood test that checks for ... How the Test is Performed. A blood sample is needed.. The sample is sent to a laboratory. There, a method called complement ...
more infohttps://www.scripps.org/articles/2672-complement-fixation-test-to-c-burnetii

Immunotyping of different strains of Japanese encephalitis virus by antibody-absorption, haemagglutination-inhibition and...Immunotyping of different strains of Japanese encephalitis virus by antibody-absorption, haemagglutination-inhibition and...

... haemagglutination-inhibition and complement-fixation tests*. Bulletin of the World Health Organization, 38 (‎4)‎, 547 - 563. ... haemagglutination-inhibition and complement-fixation tests*. ...
more infohttp://apps.who.int/iris/handle/10665/266580

The American Journal of Tropical Medicine and Hygiene | Quantitative Complement Fixation Test in Schistosomiasis MansoniThe American Journal of Tropical Medicine and Hygiene | Quantitative Complement Fixation Test in Schistosomiasis Mansoni

The test was positive in 88.9% of the children and 89.3% of the adults. The titers obtained for the two age groups did not ... Summary Complement fixation tests with a purified extract of adult Schistosoma mansoni were performed by a quantitative ... Quantitative Complement Fixation Test in Schistosomiasis Mansoni * Authors: J. Pellegrino, J. L. Pedreira de Freitas ... Complement fixation tests with a purified extract of adult Schistosoma mansoni were performed by a quantitative technique with ...
more infohttp://www.ajtmh.org/content/journals/10.4269/ajtmh.1961.10.537

The American Journal of Tropical Medicine and Hygiene | Micro-Kolmer Complement Fixation Test for AmebiasisThe American Journal of Tropical Medicine and Hygiene | Micro-Kolmer Complement Fixation Test for Amebiasis

Results on 303 cases tested for amebiasis with this method show that an amebic complement fixation test can be useful in ... modification of the Kolmer-Wassermann complement fixation method, for the detection of Endamoeba histolytica antibodies is ... Results on 303 cases tested for amebiasis with this method show that an amebic complement fixation test can be useful in ... A Micro-Kolmer technique, modification of the Kolmer-Wassermann complement fixation method, for the detection of Endamoeba ...
more infohttp://www.ajtmh.org/content/journals/10.4269/ajtmh.1952.1.717

Complement fixation test - WikipediaComplement fixation test - Wikipedia

The complement fixation test is an immunological medical test that can be used to detect the presence of either specific ... The basic steps of a complement fixation test are as follows: Serum is separated from the patient. Patients naturally have ... Complement Fixation Tests at the US National Library of Medicine Medical Subject Headings (MeSH) http://medmicro.atwebpages.com ... To negate any effects this might have on the test, the complement proteins in the patients serum must be destroyed and ...
more infohttps://en.wikipedia.org/wiki/Complement_fixation_test

Filarial complement-fixation test for pulmonary tropical eosinophilia with Ascaris antigen | Journal of Clinical PathologyFilarial complement-fixation test for pulmonary tropical eosinophilia with Ascaris antigen | Journal of Clinical Pathology

If patients could recognise themselves, or anyone else could recognise a patient from your description, please obtain the patients written consent to publication and send them to the editorial office before submitting your response [Patient consent forms] ...
more infohttps://jcp.bmj.com/content/21/3/394.responses

Texas Department of State Health Services - The complement fixation testTexas Department of State Health Services - The complement fixation test

... test is based on the use of complement, a biological substance present in the sera of normal animals. Its great value is its ... The complement fixation test is based on the use of complement, a biological substance present in the sera of normal animals. ... render the complement fixation test rather difficult and time consuming. An actual test requires 2 days to complete. It is this ... In the Molecular and Serological Analysis Group a specially standardized complement fixation test, the Laboratory Branch ...
more infohttps://www.dshs.state.tx.us/LAB/serology_cf.shtm

Detection of Antibodies against Mycoplasma mycoides subsp. capri in Goats with the Complement Fixation TestDetection of Antibodies against Mycoplasma mycoides subsp. capri in Goats with the Complement Fixation Test

... was screened by the Complement Fixation test (CF) in sera from asymptomatic goats and animals with mild respiratory symptoms. ... The CF registered 251 positive goats (30.35%) and 576 (69.65%) negative; the test showed high sensitivity (93.33%) and ... the CF proved to be a good diagnosis test, this study determined that 30% of the goat population showed antibody titers against ... capri in Goats with the Complement Fixation Test. José L. Corona-Vargas1, Myrna A. Vicencio-Mallén1, Frida Salmerón-Sosa1, ...
more infohttps://file.scirp.org/Html/4-2270831_72189.htm

Complement fixation test - ?? ????  ???????? ????Complement fixation test - ?? ???? ???????? ????

Platelets Count Test. Fragility Test. Urine Culture. ??? :????? ??? ????????? ( Insulin). ??? : ???????? ????????? Sex Hormones ... Complement fixation test ??? ?? ?? ????? ?? ?? COMPLEMENT FIXATION TEST ?????? ... Foam test, strip test And Ictotest for bilirubin Dr. Dr. ?? ??????? ??????? ????????? [Biochemistry & Hormones]. 4. 09-25-2009 ... The Complement System 2 Portrait. ?? ??????? ???????? [Immunology & Serology]. 6. 01-16-2011 07:24 PM. ...
more infohttp://www.aama-a.com/vb/showthread.php?t=4585

Complement Fixation Test | Chronic HepatitisComplement Fixation Test | Chronic Hepatitis

Complement fixation test, Gel diffusion test, infectious disease, Leptospirosis, Liver function test, liver infection, Nasal ... ammonia Appetite Loss blood test chronic infection coma dark urine Diarrhea disease DNA Fatigue fever Headache Hepatic ... Tagged as : Acute form, Animal inoculation test, canine health specialist, Canine Hepatitis, ... discharge, Neutralization test, Sore throat, Swollen lymph nodes, toxic, vaccinations, Warfarin poisoning. ...
more infohttp://chronichepatitis.org/tag/complement-fixation-test/

Effects of phenol on card-agglutination and complement-fixation tests for bovine anaplasmosis. - Semantic ScholarEffects of phenol on card-agglutination and complement-fixation tests for bovine anaplasmosis. - Semantic Scholar

... and for the micro-complement-fixation test (CFT) for bovine anaplasmosis were studied. Sera were obtained from 14 recently ... Aliquots of sera were tested with and without phenol (0.25% final concentration). Phenol adversely affected the CAT by causing ... Effects of adding phenol to sera used for the card-agglutination test (CAT) ... Effects of adding phenol to sera used for the card-agglutination test (CAT) and for the micro-complement-fixation test (CFT) ...
more infohttps://www.semanticscholar.org/paper/Effects-of-phenol-on-card-agglutination-and-comple-Amerault-Rose/0e9a568c4e9399a3a60a047f825e261dcffdb7be

Complement Fixation Test : principle, procedure and interpretation | LaboratoryInfo.comComplement Fixation Test : principle, procedure and interpretation | LaboratoryInfo.com

This test is based on the use of ... The complete fixation test (CFT) is used to detect the presence ... Principle of complement fixation test. It is the nature of the complement to be activated when there is formation of antigen- ... Complement Fixation Test : principle, procedure and interpretation. By Editorial Team on January 6, 2019 in Bacteriology, ... So the test is positive for presence of antibodies.. In the negative test : No Ag-Ab reaction occurs and the complement is free ...
more infohttps://laboratoryinfo.com/complement-fixation-test/

A comparison of the complement fixation and immunofluorescent antibody tests in a survey of the prevalence ofBabesia equi...A comparison of the complement fixation and immunofluorescent antibody tests in a survey of the prevalence ofBabesia equi...

... été recherchée par les tests de fixation du complément (CF) et des anticorps fluorescents (IFA). Deux séries déchantillons ... A group of animals tested within 4 months of importation gave 60.4% positive reactions by IFA and 62.5% by CF toB. equi; 6.3% ... The discrepancy between the results obtained by the 2 serological tests was probably due to the greater sensitivity of the IFA ... test and the faster decay of the antibodies detectable by the CF test. La présence danticorps contreBabesia equi etBabesia ...
more infohttps://www.semanticscholar.org/paper/A-comparison-of-the-complement-fixation-and-tests-a-Donnelly-Joyner/802d3968e2dbab57d9cb33817f68a5f53dd2859b

CNS  Flashcards by Aron Chacko | BrainscapeCNS Flashcards by Aron Chacko | Brainscape

Rabies virus neutralizing Ab - CSF or serum (unimmunized) Direct Ab Test. 3 ...
more infohttps://www.brainscape.com/flashcards/cns-2312075/packs/3955489

Mycoplasma Flashcards by Dimitrios Drekolias | BrainscapeMycoplasma Flashcards by Dimitrios Drekolias | Brainscape

2. Complement fixation test. 3. Culture 4. Rapid identification tests 5 How can we culture M.pneumoniae? ... Sputum can be tested with DNA probes (nucleic acid hybridization). PCR of sputum samples. ...
more infohttps://www.brainscape.com/flashcards/mycoplasma-2464686/packs/2562574
  • These standardizations, which involve titrations of sheep red blood cells, hemolysin, complement, and antigens before the test proper can be performed, render the complement fixation test rather difficult and time consuming. (tx.us)
  • It is this reason, as well as the fact that many of the test antigens and antisera are not available commercially, that the test is not often incorporated into the test program of the average clinical laboratory. (tx.us)
  • A test apparatus for the determination of immunoassays of antigens and their antibodies which comprises a receptacle tray having a plurality of wells for receiving and samples, a holder for receiving and holding balls, coated with an immunologic composition and for depositing the balls into the wells. (google.com)
  • Viral hepatitis, including so called serum hepatitis, which is a relatively common disease, has not been heretofore easily detected by a sensitive test which is both specific and reproducible for quickly determining whether or not the serum from a patient or a donor contains hepatitis associated antigens or antibodies. (google.com)
  • p = 0.000) and a high correspondence between high RBT scores and positive FPA results suggesting that sera with high RBT score may not require confirmation with tests such as competitive-ELISA or CFT. (springer.com)
  • Effectiveness of Rose Bengal test and fluorescence polarization assay in the diagnosis of Brucella spp. (springer.com)
  • The effectiveness of Rose Bengal test (RBT) and fluorescence polarization assay (FPA) in diagnosing cattle brucellosis in endemic areas was assessed and RBT and FPA test agreement was compared (n = 319). (springer.com)
  • Validation of a fluorescence polarization assay (FPA) performed in microplates and comparison with other tests used for diagnosing B. melitensis infection in sheep and goats. (springer.com)
  • Although there have been methods for determining the presence of antigenically active macromolecules such as intact viruses, virus capsids, subunits, bacteria, membranes, cell walls, hormones, etc., there has been a lack of a simple yet sensitive test method and apparatus for determining the presence of these materials. (google.com)
  • This test checks if your central nervous system has an active infection from coccidioides. (medlineplus.gov)
  • An abnormal spinal fluid test means that the central nervous system is infected. (medlineplus.gov)
  • A logistic regression model was constructed to predict cattle test result in FPA using RBT as the main predictor and incorporating bio-data and animal history. (springer.com)
  • An actual test requires 2 days to complete. (tx.us)