AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesAnthropology, Education, Sociology and Social PhenomenaInformation ScienceNamed GroupsHealth Care
Comet AssayDNA DamageMicronucleus TestsMutagensMutagenicity TestsDNA-Formamidopyrimidine GlycosylaseMeteoroidsAntimutagenic AgentsCosmic DustDNA RepairDNA Breaks, Single-StrandedLymphocytesDNA BreaksMicronuclei, Chromosome-DefectiveNeonGamma RaysEthyl MethanesulfonateDNA FragmentationDNAMethyl MethanesulfonateLeukemia L5178Dose-Response Relationship, RadiationElectrophoresis, Agar GelOxidative StressDNA Breaks, Double-StrandedRadiationSpermatozoaRadiation ToleranceCaseariaHydrogen PeroxideMelastomataceaeDeoxyguanosineOccupational Exposure7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxideRadiation, IonizingStruthioniformesCell SurvivalEthylnitrosoureaAgrochemicalsAntioxidantsDose-Response Relationship, DrugMedical SecretariesCoke4-Nitroquinoline-1-oxideX-RaysEncyclopedias as TopicMedlinePlusConsumer Health Information
Comet Assay
A genotoxicological technique for measuring DNA damage in an individual cell using single-cell gel electrophoresis. Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. Alkaline assay conditions facilitate sensitive detection of single-strand damage.
DNA Damage
Injuries to DNA that introduce deviations from its normal, intact structure and which may, if left unrepaired, result in a MUTATION or a block of DNA REPLICATION. These deviations may be caused by physical or chemical agents and occur by natural or unnatural, introduced circumstances. They include the introduction of illegitimate bases during replication or by deamination or other modification of bases; the loss of a base from the DNA backbone leaving an abasic site; single-strand breaks; double strand breaks; and intrastrand (PYRIMIDINE DIMERS) or interstrand crosslinking. Damage can often be repaired (DNA REPAIR). If the damage is extensive, it can induce APOPTOSIS.
Micronucleus Tests
Induction and quantitative measurement of chromosomal damage leading to the formation of micronuclei (MICRONUCLEI, CHROMOSOME-DEFECTIVE) in cells which have been exposed to genotoxic agents or IONIZING RADIATION.
Mutagens
Chemical agents that increase the rate of genetic mutation by interfering with the function of nucleic acids. A clastogen is a specific mutagen that causes breaks in chromosomes.
Mutagenicity Tests
Tests of chemical substances and physical agents for mutagenic potential. They include microbial, insect, mammalian cell, and whole animal tests.
DNA-Formamidopyrimidine Glycosylase
A DNA repair enzyme that is an N-glycosyl hydrolase with specificity for DNA-containing ring-opened N(7)-methylguanine residues.
Meteoroids
Any solid objects moving in interplanetary space that are smaller than a planet or asteroid but larger than a molecule. Meteorites are any meteoroid that has fallen to a planetary surface. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Antimutagenic Agents
Agents that reduce the frequency or rate of spontaneous or induced mutations independently of the mechanism involved.
Cosmic Dust
Finely divided solid matter with particle sizes smaller than a micrometeorite, thus with diameters much smaller than a millimeter, moving in interplanetary space. (NASA Thesaurus, 1994)
DNA Repair
The reconstruction of a continuous two-stranded DNA molecule without mismatch from a molecule which contained damaged regions. The major repair mechanisms are excision repair, in which defective regions in one strand are excised and resynthesized using the complementary base pairing information in the intact strand; photoreactivation repair, in which the lethal and mutagenic effects of ultraviolet light are eliminated; and post-replication repair, in which the primary lesions are not repaired, but the gaps in one daughter duplex are filled in by incorporation of portions of the other (undamaged) daughter duplex. Excision repair and post-replication repair are sometimes referred to as "dark repair" because they do not require light.
DNA Breaks, Single-Stranded
Interruptions in one of the strands of the sugar-phosphate backbone of double-stranded DNA.
Lymphocytes
White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.
DNA Breaks
Interruptions in the sugar-phosphate backbone of DNA.
Micronuclei, Chromosome-Defective
Defective nuclei produced during the TELOPHASE of MITOSIS or MEIOSIS by lagging CHROMOSOMES or chromosome fragments derived from spontaneous or experimentally induced chromosomal structural changes.
Neon
Neon. A noble gas with the atomic symbol Ne, atomic number 10, and atomic weight 20.18. It is found in the earth's crust and atmosphere as an inert, odorless gas and is used in vacuum tubes and incandescent lamps.
Gamma Rays
Penetrating, high-energy electromagnetic radiation emitted from atomic nuclei during NUCLEAR DECAY. The range of wavelengths of emitted radiation is between 0.1 - 100 pm which overlaps the shorter, more energetic hard X-RAYS wavelengths. The distinction between gamma rays and X-rays is based on their radiation source.
Ethyl Methanesulfonate
An antineoplastic agent with alkylating properties. It also acts as a mutagen by damaging DNA and is used experimentally for that effect.
DNA Fragmentation
Splitting the DNA into shorter pieces by endonucleolytic DNA CLEAVAGE at multiple sites. It includes the internucleosomal DNA fragmentation, which along with chromatin condensation, are considered to be the hallmarks of APOPTOSIS.
DNA
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
Methyl Methanesulfonate
An alkylating agent in cancer therapy that may also act as a mutagen by interfering with and causing damage to DNA.
Leukemia L5178
An experimental lymphocytic leukemia of mice.
Dose-Response Relationship, Radiation
The relationship between the dose of administered radiation and the response of the organism or tissue to the radiation.
Electrophoresis, Agar Gel
Electrophoresis in which agar or agarose gel is used as the diffusion medium.
Oxidative Stress
A disturbance in the prooxidant-antioxidant balance in favor of the former, leading to potential damage. Indicators of oxidative stress include damaged DNA bases, protein oxidation products, and lipid peroxidation products (Sies, Oxidative Stress, 1991, pxv-xvi).
DNA Breaks, Double-Stranded
Interruptions in the sugar-phosphate backbone of DNA, across both strands adjacently.
Radiation
Emission or propagation of acoustic waves (SOUND), ELECTROMAGNETIC ENERGY waves (such as LIGHT; RADIO WAVES; GAMMA RAYS; or X-RAYS), or a stream of subatomic particles (such as ELECTRONS; NEUTRONS; PROTONS; or ALPHA PARTICLES).
Spermatozoa
Mature male germ cells derived from SPERMATIDS. As spermatids move toward the lumen of the SEMINIFEROUS TUBULES, they undergo extensive structural changes including the loss of cytoplasm, condensation of CHROMATIN into the SPERM HEAD, formation of the ACROSOME cap, the SPERM MIDPIECE and the SPERM TAIL that provides motility.
Radiation Tolerance
The ability of some cells or tissues to survive lethal doses of IONIZING RADIATION. Tolerance depends on the species, cell type, and physical and chemical variables, including RADIATION-PROTECTIVE AGENTS and RADIATION-SENSITIZING AGENTS.
Casearia
A plant genus of the family FLACOURTIACEAE. Members contain casearins which are clerodane type DITERPENES.
Hydrogen Peroxide
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.
Melastomataceae
A plant family of the order Myrtales, subclass Rosidae, class Magnoliopsida composed of tropical plants with parallel-nerved leaves.
Deoxyguanosine
A nucleoside consisting of the base guanine and the sugar deoxyribose.
Occupational Exposure
The exposure to potentially harmful chemical, physical, or biological agents that occurs as a result of one's occupation.
7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
7,8,8a,9a-Tetrahydrobenzo(10,11)chryseno (3,4-b)oxirene-7,8-diol. A benzopyrene derivative with carcinogenic and mutagenic activity.
Radiation, Ionizing
ELECTROMAGNETIC RADIATION or particle radiation (high energy ELEMENTARY PARTICLES) capable of directly or indirectly producing IONS in its passage through matter. The wavelengths of ionizing electromagnetic radiation are equal to or smaller than those of short (far) ultraviolet radiation and include gamma and X-rays.
Struthioniformes
An order of flightless birds comprising the ostriches, which naturally inhabit open, low rainfall areas of Africa.
Cell Survival
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Ethylnitrosourea
A nitrosourea compound with alkylating, carcinogenic, and mutagenic properties.
Agrochemicals
Chemicals used in agriculture. These include pesticides, fumigants, fertilizers, plant hormones, steroids, antibiotics, mycotoxins, etc.
Antioxidants
Naturally occurring or synthetic substances that inhibit or retard the oxidation of a substance to which it is added. They counteract the harmful and damaging effects of oxidation in animal tissues.
Dose-Response Relationship, Drug
The relationship between the dose of an administered drug and the response of the organism to the drug.
Medical Secretaries
Individuals responsible for various duties pertaining to the medical office routine.
Coke
A residue of coal, left after dry (destructive) distillation, used as a fuel.
4-Nitroquinoline-1-oxide
A potent mutagen and carcinogen. This compound and its metabolite 4-HYDROXYAMINOQUINOLINE-1-OXIDE bind to nucleic acids. It inactivates bacteria but not bacteriophage.
X-Rays
Penetrating electromagnetic radiation emitted when the inner orbital electrons of an atom are excited and release radiant energy. X-ray wavelengths range from 1 pm to 10 nm. Hard X-rays are the higher energy, shorter wavelength X-rays. Soft x-rays or Grenz rays are less energetic and longer in wavelength. The short wavelength end of the X-ray spectrum overlaps the GAMMA RAYS wavelength range. The distinction between gamma rays and X-rays is based on their radiation source.
Encyclopedias as Topic
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
MedlinePlus
NATIONAL LIBRARY OF MEDICINE service for health professionals and consumers. It links extensive information from the National Institutes of Health and other reviewed sources of information on specific diseases and conditions.
Consumer Health Information
Information intended for potential users of medical and healthcare services. There is an emphasis on self-care and preventive approaches as well as information for community-wide dissemination and use.

Vitamin E supplementation and oxidative damage to DNA and plasma LDL in type 1 diabetes. (1/1075)

OBJECTIVE: To determine the effect of 400 IU/day of the antioxidant vitamin E on the susceptibility of plasma LDL and lymphocyte DNA to oxidative damage in type 1 diabetes. RESEARCH DESIGN AND METHODS: We studied 42 patients with type 1 diabetes and 31 age- and sex-matched control subjects in a randomized prospective double-blind placebo-controlled trial by using 400 IU/day of oral vitamin E for 8 weeks. Measurements were made of single-strand breaks in lymphocyte DNA at baseline and after hydrogen peroxide-induced stress (comet assay) and of copper-induced LDL oxidization and plasma antioxidant profiles. RESULTS: Plasma LDL and lymphocyte DNA were more resistant to induced oxidative change in the type 1 diabetes group than in control subjects. Vitamin E supplementation reduced LDL oxidizability in the control subjects but not in the type 1 diabetes group and had no effect on oxidative DNA damage in either group. The type 1 diabetes group had a significantly poorer plasma antioxidant profile with lower mean serum concentrations of alpha-tocopherol and most carotenoids than control subjects. CONCLUSIONS: Plasma LDL and lymphocyte DNA appear to be more resistant to oxidative change in type 1 diabetic subjects than in control subjects, and there was no evidence of oxidatively induced DNA or LDL change in type 1 diabetes. This study does not support the hypothesis of oxidative damage in these patients, and a dose of vitamin E (400 IU/day) that reduced LDL oxidative susceptibility in control subjects did not do so in patients with type 1 diabetes.  (+info)

Comet assay application in environmental monitoring: DNA damage in human leukocytes and plant cells in comparison with bacterial and yeast tests. (2/1075)

Urban airborne particulate is a complex mixture of air pollutants, many of which have not been identified. However, short-term mutagenesis tests together with chemicophysical parameter analysis are able to better assess air quality and genotoxic load. The findings of continuous monitoring (January 1991-August 1998) of urban air genotoxicity of a Po Valley town (Italy) on Salmonella typhimurium and Saccharomyces cerevisiae are reported. During this period, various measures (catalytic devices, unleaded fuels, annual vehicle overhaul, etc.) to improve air-dispersed pollutant control were enforced. However, a continuous presence of genotoxic compounds is shown and more qualitative than quantitative changes are evident. We also demonstrate the ability of the Comet assay to detect DNA-damaging agents in airborne particulate samples. We applied the test to human leukocytes and, with major improvements, to plant cells (Allium cepa roots and epigean tissues of Impatiens balsamina). The first findings on human leukocytes confirm the sensitivity of this assay, its peculiarity and its applicability in assessing genotoxicity in environmental samples. The capability of plants to show the response of multicellular organisms to environmental pollutants largely counterbalances a probable lowering in sensitivity. Moreover, application of the Comet test to epigean tissues could be useful in estimating the bioavailability of and genotoxic damage by air pollutants, including volatile compounds (ozone, benzene, nitrogen oxides, etc.) to higher plants.  (+info)

Comet assay of UV-induced DNA damage in retinal pigment epithelial cells. (3/1075)

PURPOSE: The molecular mechanisms mediating photic injury to the retinal pigment epithelial (RPE) cell are not clearly understood. This study examined qualitative and quantitative aspects of DNA damage caused by broadband UVA and UVB radiation in RPE cells. METHODS: Cultured bovine RPE cells were exposed to doses of between 0 and 0.9 J/cm2 UVA or 0 and 0.09 J/cm2 UVB, as either a suspension or as an attached monolayer. The damage to DNA resulting in single-strand breaks was assessed by means of the comet assay in which the damaged DNA migrates out of the nucleus forming a tail, and this was quantified using image analysis. Two measurements were taken: the mean percentage of tail DNA, which reflects the overall level of DNA damage in the group of cells, and the Olive tail moment, which represents the extent of migration and thus the pattern of DNA damage in individual cells. Cells were processed by the comet assay immediately after UV exposure in acute experiments. To study the occurrence of DNA repair, RPE cells were first exposed to UVB and then incubated at 37 degrees C for either 1 or 24 hours before processing for the comet assay. RESULTS: UVA- and UVB-exposed cells showed a mean percentage of tail DNA that was significantly greater than in unexposed cells. Olive tail moment was higher in cells exposed to larger doses of UVB. This parameter also showed a bimodal distribution when assessed 24 hours after exposure to UVB indicating the presence of two distinct subpopulations of cells with small and large tail moments. Cells with very large tail moments were not seen with doses below 0.045 J/cm2. CONCLUSIONS: Relatively low doses of UVA and UVB induce the formation of DNA strand breaks in cultured RPE. The tail moment profiles for cells incubated for 24 hours after UVB irradiation are consistent with the occurrence of DNA repair in most cells exposed to low doses and apoptosis in a subpopulation of the cells exposed to high doses.  (+info)

The bromodeoxyuridine comet assay: detection of maturation of recently replicated DNA in individual cells. (4/1075)

The single-cell gel electrophoresis (Comet) assay is a relatively simple method of measuring DNA single strand breaks and alkali-labile sites in individual cells. We have combined this with bromodeoxyuridine (BrdUrd) labeling of DNA and immunolocalization of the BrdUrd to assess DNA replicative integrity on a single-cell basis. We show that the existence of strand discontinuities in recently replicated domains of DNA, caused during semiconservative replication or exacerbated by the arrest of replicative polymerases at UV irradiation- or chemical-induced lesions, can be detected in individual cells. Data obtained from BrdUrd-Comets are consistent with biochemical data derived with a range of techniques showing that DNA replication involves the creation of strand breaks or gaps adjacent to recently replicated material, and that DNA damage prolongs the duration of such discontinuities where DNA polymerases are stalled opposite lesions (R. T. Johnson et al, The Legacy of Cell Fusion, pp. 50-67, Oxford: Science Publications, 1994; R. B. Painter, J. Mol. Biol., 143: 289-301, 1980.). Compared with standard biochemical techniques, the BrdUrd-Comet assay is simple and suitable for the accurate and automatable assessment of replicative integrity in very small numbers of mammalian cells, such as may be obtained by biopsy.  (+info)

Extremely low frequency pulsed DC electric fields promote neutrophil extension, metabolic resonance and DNA damage when phase-matched with metabolic oscillators. (5/1075)

Application of extremely low frequency pulsed DC electric fields that are frequency- and phase-matched with endogenous metabolic oscillations leads to greatly exaggerated neutrophil extension and metabolic resonance wherein oscillatory NAD(P)H amplitudes are increased. In the presence of a resonant field, migrating cell length grows from 10 to approximately 40 microm, as does the overall length of microfilament assemblies. In contrast, cells stop locomotion and become spherical when exposed to phase-mismatched fields. Although cellular effects were not found to be dependent on electrode type and buffer, they were sensitive to temporal constraints (phase and pulse length) and cell surface charge. We suggest an electromechanical coupling hypothesis wherein applied electric fields and cytoskeletal polymerization forces act together to overcome the surface/cortical tension of neutrophils, thus promoting net cytoskeletal assembly and heightened metabolic amplitudes. Metabolic resonance enhances reactive oxygen metabolic production by neutrophils. Furthermore, cellular DNA damage was observed after prolonged metabolic resonance using both single cell gel electrophoresis ('comet' assay) and 3'-OH DNA labeling using terminal deoxynucleotidyl transferase. These results provide insights into transmembrane signal processing and cell interactions with weak electric fields.  (+info)

DNA damage induced by 3,3'-dimethoxybenzidine in liver and urinary bladder cells of rats and humans. (6/1075)

3,3'-Dimethoxybenzidine (DMB), a congener of benzidine used in the dye industry and previously found to be carcinogenic in rats, was evaluated for its genotoxic activity in primary cultures of rat and human hepatocytes and of cells from human urinary bladder mucosa, as well as in liver and bladder mucosa of intact rats. A similar modest dose-dependent frequency of DNA fragmentation was revealed by the alkaline elution technique in metabolically competent primary cultures of both rat and human hepatocytes exposed for 20 h to subtoxic DMB concentrations ranging from 56 to 180 microM. Replicating rat hepatocytes displayed a modest increase in the frequency of micronucleated cells after a 48-h exposure to 100 and 180 microM concentrations. In primary cultures of human urinary bladder mucosa cells exposed for 20 h to 100 and 180 microM DMB, the Comet assay revealed a clear-cut increase of DNA fragmentation. In rats given one-half LD50 of DMB as a single oral dose, the GSH level was reduced in both the liver and urinary bladder mucosa, whereas DNA fragmentation was detected only in the bladder mucosa. Taken as a whole, these results suggest that DMB should be considered a potentially genotoxic chemical in both rats and humans; the selective effect on the rat urinary bladder might be the consequence of pharmacokinetic behavior.  (+info)

Induction of genotoxicity by cadmium chloride inhalation in several organs of CD-1 mice. (7/1075)

In recent years, the concentration of metals in the environment has increased significantly. Metal compounds, as a group, are among the best-documented human carcinogens, but the mechanisms by which they act are not completely understood. In the present study a cadmium inhalation model in mice was implemented in order to detect the induction of genotoxic damage as single-strand breaks and alkali-labile sites in several organs (nasal epithelial cells, lung, whole blood, liver, kidney, bone marrow, brain and testicle) using the single cell gel electrophoresis (SCGE) or Comet assay. We found differences among the studied organs after a single and subsequent inhalations: in the organs analyzed we observed that major DNA damage was induced after a single inhalation; in subsequent inhalations there was a tendency to maintain the same magnitude of damage or in some cases it decreased. A correlation between length of exposure, DNA damage and metal tissue concentration was found. These results suggest that cadmium chloride inhalation induces systemic DNA damage; some organs showed less damage than others (liver, brain, etc.) and this finding could be as a consequence of the capacity to remove the damage induced by long periods of exposure, possibly because of the induction of detoxifying mechanisms such as induction of metallothionein.  (+info)

Lymphocyte lycopene concentration and DNA protection from oxidative damage is increased in women after a short period of tomato consumption. (8/1075)

Several epidemiologic studies have suggested a role of tomato products in protecting against cancer and chronic diseases. In nine adult women, we evaluated whether the consumption of 25 g tomato puree (containing 7 mg lycopene and 0.3 mg beta-carotene) for 14 consecutive days increased plasma and lymphocyte carotenoid concentration and whether this was related to an improvement in lymphocyte resistance to an oxidative stress (500 micromol/L hydrogen peroxide for 5 min). Before and after the period of tomato intake, carotenoid concentrations were analyzed by HPLC and lymphocyte resistance to oxidative stress by the Comet assay, which detects DNA strand breaks. Intake of tomato puree increased plasma (P <0.001) and lymphocyte (P<0.005) lycopene concentration and reduced lymphocyte DNA damage by approximately 50% (P<0.0001). Beta-carotene concentration increased in plasma (P<0.05) but not in lymphocytes after tomato puree consumption. An inverse relationship was found between plasma lycopene concentration (r = -0.82, P<0.0001) and lymphocyte lycopene concentration (r = -0.62, P<0.01) and the oxidative DNA damage. In conclusion, small amounts of tomato puree added to the diet over a short period can increase carotenoid concentrations and the resistance of lymphocytes to oxidative stress.  (+info)

Analysis of DNA damage in cells excreted in urine of cervical cancer patients using alkaline comet assay | Molecular...Analysis of DNA damage in cells excreted in urine of cervical cancer patients using alkaline comet assay | Molecular...
Cancer is one of the most unwanted menaces in the human body. Cancers of lower abdomen are not only life threatening but also painful and survival rate is low. Cervical cancer is second most common worldwide and fifth deadliest in women. It affects about 16 per 100,000 women per year and kills about 9 per 100,000 in a year. In developing countries occurrence rate is 80%. It is possible that there may be no symptom until an advance stage of the cancer is progressed. The single cell gel electrophoresis assay also called comet assay, which is versatile, reliable, powerful, uncomplicated and sensitive technique for detection of DNA damage at the level of individual eukaryotic cell. Understanding the extent of DNA damage in neoplastic cells discarded in the urine of cancer patients through comet assay. Usually normal urine sample have very rare cells. In case of cancer patient the number of cells increases drastically. The type of cells passed in the urine of cancer patient contains mainly ...
https://molecularcytogenetics.biomedcentral.com/articles/10.1186/1755-8166-7-S1-P12
Comet Assay | Single Cell Gel Electrophoresis | Sigma-AldrichComet Assay | Single Cell Gel Electrophoresis | Sigma-Aldrich
The Comet Assay, also called single cell gel electrophoresis (SCGE), is a sensitive and rapid technique for quantifying and analyzing DNA damage in individual cells.
https://www.sigmaaldrich.com/life-science/cell-biology/cancer-research/learning-center/cancer-research-protocols/comet-assay.html
Low molecular weight DNA diffusion (LMW) and comet assays
Recently, investigators at Universität Ulm have been investigating the low molecular weight DNA diffusion assay (LMW assay) to measure cytotoxicity in comet assays.. The comet assay is a well-established in vitro and in vivo genotoxicity test. However, there has always been concern that the comet assay (as with all DNA strand break assays) may possibly detect exposure-related cytotoxicity rather than genotoxic effects. This is because DNA degradation is frequently involved in processes leading to cell death. Concurrent measures for cytotoxicity are required for standard genotoxicity testing to assess genotoxicity and to address possible effects of cytotoxicity in comet assay data interpretation.. Typically, histopathology has been accepted as a suitable cytotoxicity detection method when using the in vivo comet assay. By looking at the histology, levels of necrosis and apoptosis can be determined. However, there are other assays available for assessing cytotoxicity.. Here, the LMW assay has ...
http://www.scorecomets.com/comet-assay-news/190-lmw-and-comet-assays
Sevoflurane induces DNA damage whereas isoflurane leads to higher antioxidative status in anesthetized ratsSevoflurane induces DNA damage whereas isoflurane leads to higher antioxidative status in anesthetized rats
Taking into account that there are controversial antioxidative effects of inhalational anesthetics isoflurane and sevoflurane and absence of comparison of genotoxicity of both anesthetics in animal model, the aim of this study was to compare DNA damage and antioxidant status in Wistar rats exposed to a single time to isoflurane or sevoflurane. The alkaline single-cell gel electrophoresis assay (comet assay) was performed in order to evaluate DNA damage in whole blood cells of control animals (unexposed; n = 6) and those exposed to 2% isoflurane (n = 6) or 4% sevoflurane (n = 6) for 120 min. Plasma antioxidant status was determined by 3( 4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. There was no statistically significant difference between isoflurane and sevoflurane groups regarding hemodynamic and temperature variables (P > 0.05). Sevoflurane significantly increased DNA damage compared to unexposed animals (P = 0.02). In addition, Wistar rats anesthetized with ...
https://repositorio.unesp.br/handle/11449/128572
Toxics | Free Full-Text | Cytotoxic, Apoptotic and Genotoxic Effects of Lipid-Based and Polymeric Nano Micelles, an In Vitro...Toxics | Free Full-Text | Cytotoxic, Apoptotic and Genotoxic Effects of Lipid-Based and Polymeric Nano Micelles, an In Vitro...
Self-assembly systems (SAS) mainly consist of micelles, and liposomes are the classes of Nano Drug Delivery Systems with superior properties compared to traditional therapeutics in targeting cancer tumors. All commercially available nano-formulations of chemotherapeutics currently consist of SAS. According to our knowledge, a specific toxicity comparison based on material differences has not yet been performed. The purpose of this study was to evaluate and compare the toxicity of two SAS consisting of Sterically Stabilized Micelles (SSM) made of a lipid-based amphiphilic distearoyl-sn-glycero-phosphatidylethanolamine-polyethylene glycol (PEG)-2000 and a polymeric micelle (PM) consisting of Y-shape amphiphilic block copolymer, synthesized using poly ε-caprolactone and PEG. The mechanism of cytotoxicity and genotoxicity of micelles on L-929 healthy mouse fibroblast cells was assessed using Sulforhodamine-B, WST-1, Acridine Orange/Ethidium Bromide and alkaline single-cell gel electrophoresis assays.
http://www.mdpi.com/2305-6304/6/1/7
Effects of proton beams from the folded tandem ion accelerator on DNA damage in mouse leukocytes using the comet assay on...Effects of proton beams from the folded tandem ion accelerator on DNA damage in mouse leukocytes using the comet assay on...
Article Effects of proton beams from the folded tandem ion accelerator on DNA damage in mouse leukocytes using the comet assay. Single cell gel electrophoresis or the comet assay is a sensitive technique to quantify different types of DNA damage, e.g...
https://www.environmental-expert.com/articles/effects-of-proton-beams-from-the-folded-tandem-ion-accelerator-on-dna-damage-in-mouse-leukocytes-usi-84376
Asian Science Citation Index - Articles written by Hany M. HassanAsian Science Citation Index - Articles written by Hany M. Hassan
The target of the present study is to estimate the in vitro genotoxic potential of the volatile-anaesthetic halothane (Narcotan7) as well as the protective effect of vitamin A, as an antioxidant. To achieve this task, the alkaline single cell gel electrophoresis (comet assay) was applied as the extent of DNA fragmentation in rabbit lymphocytes that were exposed in vitro to halothane at concentrations of 0.1 or 1.0 mM for 10 and 30 min, respectively. The obtained results elucidated that anaesthetic halothane induced a significant increase in DNA damage that shown by comet DNA concentration and its tail length. The recorded elevation of DNA damage was clearly correlated with halothane concentration as well as the period of exposure. To mend the deleterious effect of halothane on DNA, 25 rabbits were orally received vitamin A (8000 IU kg-1 body weight) for 15 days prior to in vitro halothane exposure. Vitamin A administration induced a significant repairing effect in lymphocytes-damaged DNA due to ...
https://scialert.net/asci/author.php?ascicat=ALL&author=Hany&last=M.%20Hassan
Cytogentic analysis of human dermal fibroblasts (HDFs) in early and late passages using both karyotyping and comet assay...Cytogentic analysis of human dermal fibroblasts (HDFs) in early and late passages using both karyotyping and comet assay...
Human dermal fibroblasts (HDFs) are a potential source of somatic cells for genetic manipulation and tissue engineering. Confirmation of cytogenetic stability of these cells is an essential step for cell nuclear transfer and generation of a suitable and functional induced pluripotent stem cells line. HDF cells were isolated and cultured from human foreskin samples. Cytogenetic stability of these cells was evaluated in early (3-4) and late (10-15) passages using karyotype test and alkaline comet assay techniques. HDF cells in early and late passages showed normal karyotype but by comet assay abnormality and DNA damages in late passages of HDFs were observed. Also, the parameters of alkaline comet assay in early passages of HDFs compared with late passages and positive control groups more significantly were different (p , 0.05). These findings indicate that single-strand breaks or DNA damage after many passages may have occurred in HDF cells. Our results demonstrate that only early passages of HDF ...
http://eprints.skums.ac.ir/2062/
北京大学医学部机构知识库([email protected]): Study on DNA strand breaks induced by sodium nitroprusside, a nitric oxide donor, in vivo and in vitro
Nitric oxide (NO) as well as its donors has been shown to generate mutation and DNA damage in in vitro assays. The objective of this study was to identify that DNA single-strand breaks (SSBs) could be elicited by NO, not only in vitro but also in vivo. The alkaline single-cell gel electrophoresis (SCGE) was performed to examine the DNA damage in g12 cells and the cells isolated from the organs of mice exposed to sodium nitroprusside (SNP). A modified method, in which neither collagenase nor trypsin was necessary, was used to prepare the single-cell suspension isolated from organs of mice. Results showed that the exposure of g12 cells to 0.13-0.5 mu mol/ml SNP with S9 for 1 h induced a concentration-dependent increase in DNA SSBs in g12 cells. The significant increase in DNA migration and comet frequency has appeared in the cells isolated from the spleen, thymus, and peritoneal macrophages of mice after injecting i.p. SNP in the dosage range of 0.67-6.0 mg/kg b.wt for 1 h. However, no obvious ...
http://ir.bjmu.edu.cn/handle/400002259/53728
IJERPH | Free Full-Text | Genotoxicity in child populations exposed to Polycyclic Aromatic Hydrocarbons (PAHs) in the air from...IJERPH | Free Full-Text | Genotoxicity in child populations exposed to Polycyclic Aromatic Hydrocarbons (PAHs) in the air from...
The economy of the state of Tabasco is based on oil extraction. However, this imposes major effects to the environment and communities. Examples are the Polycyclic Aromatic Hydrocarbons (PAHs) that may be found in the soil, water and sediment of the region. Their volatility makes them available to living beings and results in genotoxic activity. The purpose of this study was to quantify the levels of PAHs in the air at several points in the state, and to analyze their relationship with possible damage to DNA on local inhabitants. Single Cell Gel Electrophoresis Assay (Comet Assay) was applied to peripheral blood lymphocytes of five groups of children between six and 15 years of age. PAH samples were analyzed following US/EPA TO-13-A method. Results indicated the presence in the air of most of the 16 PAHs considered as high priority by EPA, some of which have been reported with carcinogenic activity. Differences (p
http://www.mdpi.com/1660-4601/5/5/349
Alkaline Comet Assay using the monocytic cell line THP-1 | Protocol Exchange on Research SquareAlkaline Comet Assay using the monocytic cell line THP-1 | Protocol Exchange on Research Square
This protocol details the experimental procedure for performing the comet assay, a very sensitive DNA break assay based on single cell gel electrophoresis.The analysis of DNA strand breaks, both single- and double-strand breaks (SSBs and DSBs, respectively), was performed in immune resp...
https://protocolexchange.researchsquare.com/article/pex-605/v2
Institute of Cancer Research Repository - Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of,...
Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk. Breast cancer may be initiated by environmental/dietary agents and human milk may act as an ex vivo indicator of in vivo exposure of mammary epithelial cells to genotoxins. Extracts of human milk from UK-resident women (n = 7) were tested for their abilities to morphologically transform C3H/M2 mouse fibroblasts. Genotoxicities were assessed in the Salmonella typhimurium reverse-mutation assay in the presence of S9 using strains TA1538 and YG1019, and in metabolically-competent human MCL-5 cells with the micronucleus and with the alkaline single cell gel electrophoresis (comet) assays. Two of the seven extracts were inactive in the transformation assay both in the presence or absence of S9, two appeared to be equally transforming either in the presence or absence of S9, and two other extracts induced increased transformation frequencies in the presence of S9. A seventh extract, tested only in ...
http://publications.icr.ac.uk/352/
Growth kinetics in MCF-7 cells modulate benzoapyrene-induced CYP1A1 up-regulation. - CLOK - Central Lancashire Online KnowledgeGrowth kinetics in MCF-7 cells modulate benzoapyrene-induced CYP1A1 up-regulation. - CLOK - Central Lancashire Online Knowledge
Pro-carcinogens, such as benzoapyrene (BaP), that are exogenous ligands of the aromatic hydrocarbon receptor may influence the susceptibility of target-cell populations through the up-regulation of cytochrome P450 (CYP) mixed function oxidases. We examined whether the growth kinetics of MCF-7 cells might determine the level of up-regulation of CYP1A1, CYP1A2 or CYP1B1 by BaP, and whether this could then influence subsequent levels of DNA damage. Cell cultures manipulated to be G0/G1-phase concentrated, S-phase concentrated or G2/M-phase concentrated were treated with BaP and the expression levels of CYP1A1, CYP1A2, CYP1B1, cyclin-dependent kinase inhibitor 1A CDKN1A (P21WAF1/CIP1), B-cell leukaemia/lymphoma-2 (BCL-2), and Bcl-2-associated X levels were determined. Levels of DNA damage were measured as DNA single-strand breaks (SSBs) by the alkaline single-cell gel electrophoresis (comet) assay or as DNA adducts by 32P-postlabelling analysis. BaP-induced up-regulation of CYP1A1 was ...
http://clok.uclan.ac.uk/16320/
Nazarbayev University Repository
Ecology affects human organism by causing breaks in DNA and, therefore, induce mutations and malignant cell formation. Comet assay is a quantitative method that allows to measure DNA damage in eukaryotic cells (Liao, McNutt, & Zhu, 2009). This method is widely used in areas, such as human biomonitoring, genotoxicology, and ecology. Comet assay is also called a single-cell gel electrophoresis. Thus, the products of electrophoresis are analyzed under the fluorescent light, which makes it similar to comets. DNA strand breaks are detected by comparing the intensity of the comet tail relative to the head (Collins, 2004). Probiotics containing Lactobacillus rhamnosus were used, as Lactobacilli probiotics were found to be safe and effective against urogenital diseases, food hypersensitivity, and dental caries (Lebeer, Vanderleyden, & De Keersmaeckler, 2008). The aim of the research was to study DNA protector mechanism for three probiotic products, containing L. rhamnosus ...
https://nur.nu.edu.kz/handle/123456789/553
The Comet Assay as a Rapid Test in Biomonitoring Occupational Exposure to DNA-damaging Agents and Effect of Confounding Factors...The Comet Assay as a Rapid Test in Biomonitoring Occupational Exposure to DNA-damaging Agents and Effect of Confounding Factors...
Within the last decade, the comet assay has been used with increasing popularity to investigate the level of DNA damage in terms of strand breaks and alkaline labile sites in biomonitoring studies. The assay is easily performed on WBCs and has been included in a wide range of biomonitoring studies of occupational exposures encompassing styrene, vinyl chloride, 1,3-butadiene, pesticides, hair dyes, antineoplastic agents, organic solvents, sewage and waste materials, wood dust, and ionizing radiation. Eleven of the occupational studies were positive, whereas seven were negative. Notably, the negative studies appeared to have less power than the positive studies. Also, there were poor dose-response relationships in many of the biomonitoring studies. Many factors have been reported to produce effects by the comet assay, e.g., age, air pollution exposure, diet, exercise, gender, infection, residential radon exposure, smoking, and season. Until now, the use of the comet assay has been hampered by the ...
https://cebp.aacrjournals.org/content/9/10/1005.abstract
Comet assay results, measurements and data processing
Comet Assay IV combines accurate, repeatable results with powerful data processing and comprehensive presentation. Once you have obtained your results, you can easily process them using the Spreadsheet Generator, which is included free with every Comet Assay IV system.. The Spreadsheet Generator is an application built in Excel for drawing graphs, tabulating data and calculating statistics from your Comet Assay IV results. Because it is built in Excel, it can easily be customised using any of Excels standard functions and features.. ...
http://www.scorecomets.com/comet-scoring/comet-assay-iv/results
Leicester Research Archive: Assessment of Phytochemicals in Preventing Oxidatively Damaged DNA in Bladder Cancer
Oxidatively damaged DNA is thought to be important in both the initiation and development of bladder carcinoma. Phytochemical compounds are thought to promote optimal health, partly via their antioxidant effects in protecting cellular components against damaging free radicals. The objective of this study is to assess the effect of a standardised bilberry extract, mirtoselect, on the level of endogenous and induced oxidatively damaged DNA in bladder cancer cells, as assessed by the Comet assay. The Comet assay, also known as single-cell gel electrophoresis, represents a simple method for measuring DNA strand break damage in eukaryotic cells. The sensitivity and specificity of the assay is greatly enhanced by the addition of bacterial repair endonucleases that recognise specific types of damage in the DNA and converts these lesions into additional DNA breaks. - Studies of mirtoselect against three bladder cancer cell lines (RT112, RT4 and HT1376) have shown significant antiproliferative activities ...
https://lra.le.ac.uk/handle/2381/10158
comet assay | Scientist Solutionscomet assay | Scientist Solutions
We present a procedure for the comet assay, a gel electrophoresisbased method that can be used to measure DNA damage in individual eukaryotic cells. It is versatile, relatively simple to perform and sensitive. Although most investigations make use of its ability to measure DNA single-strand breaks, modifications to the method allow detection of DNA double-strand breaks, cross-links, base damage and apoptotic nuclei. The limit of sensitivity is approximately 50 strand breaks per diploid mammalian cell. DNA damage and its repair in single-cell suspensions prepared from yeast, protozoa, plants, invertebrates and mammals can also be studied using this assay. Originally developed to measure variation in DNA damage and repair capacity within a population of mammalian cells, applications of the comet assay now range from human and sentinel animal biomonitoring (e.g., DNA damage in earthworms crawling through toxic waste sites) to measurement of DNA damage in specific genomic sequences. This protocol ...
http://www.scientistsolutions.com/forum/genetics-assay-development-protocols/comet-assay-0
DNA damage and breast cancer risk<...
TY - JOUR. T1 - DNA damage and breast cancer risk. AU - Smith, Tasha R.. AU - Miller, Mark S.. AU - Lohman, Kurt K.. AU - Case, L. Douglas. AU - Hu, Jennifer H.. PY - 2003/5/1. Y1 - 2003/5/1. N2 - To evaluate whether deficient DNA repair contributes to elevated DNA damage and breast carcinogenesis, we used the comet assay (single-cell alkaline gel electrophoresis) to measure the levels of DNA damage in peripheral lymphocytes from 70 breast cancer cases and 70 controls. DNA damage, measured as the comet tail moment, was not influenced by age, family history (FH), age at menarche, age at first birth or parity. The results showed that cancer cases had significantly higher DNA damage compared with controls; the comet tail moments (mean ± SD) for cases and controls were: 10.78 ± 3.63 and 6.86 ± 2.76 (P , 0.001) for DNA damage at baseline (DB), 21.24 ± 4.88 and 14.97 ± 4.18 (P , 0.001) for DNA damage after exposure to 6 Gy of ionizing radiation (DIR), and 14.76 ± 5.35 and 9.75 ± 3.35 (P , ...
https://miami.pure.elsevier.com/en/publications/dna-damage-and-breast-cancer-risk
Genotoxicity testing of two lead-compounds in somatic cells of Drosophila melanogasterGenotoxicity testing of two lead-compounds in somatic cells of Drosophila melanogaster
The in vivo genotoxic activity of two inorganic lead compounds was studied in Drosophila melanogaster by measurement of two different genetic endpoints. We used the wing-spot test and the comet assay. The comet assay was conducted with larval haemocytes. The results from the wing-spot test showed that neither lead chloride, PbCl2, nor lead nitrate, Pb(NO3)2, were able to induce significant increases in the frequency of mutant spots. In addition, the combined treatments with gamma-radiation and PbCl2 or Pb(NO3)2 did not show significant variations in the frequency of the three categories of mutant spots recorded, compared with the frequency induced by gamma-radiation alone. This seems to indicate that the lead compounds tested do not interact with the repair of the genetic damage induced by ionizing radiation. When the lead compounds were evaluated in the in vivo comet assay with haemocytes, Pb(NO3)2 was effective in inducing significant increases of DNA damage with a direct dose-response ...
http://repositoriodigital.uct.cl/handle/10925/552
CometAssay Electrophoresis Starter Kit plus Power Supply for EU (except UK Switzerland) | Technique alternative | 01011887528 -...
The Comet Assay for detection of DNA repair and DNA damage by tail intensity. In Comet Assays the damage is detected with the single-stranded or double-stranded DNA breaks. DNA damage can be induced by chemicals or UV light radiation. A single cell is paced in low melting agarose on a microscope slide. The cell membrane will be lysed with detergent and salt concentration. Nucleotides will be released from the nucleus and electrophoresis will migrate the damaged DNA to the + anode electric field. The more the DNA is damaged the more there will appear a migration tail. Endonucleases damage increases the DNA migration, whereas DNA-DNA and DNA-protein cross-links result in retarded DNA migration, Tice, 2000. The comet assay is used in DNA repair studies, in animal and clinical studies for base excision repair (BER), nucleotide excision repair (NER) like the Vitotox L. Gevaert, 2009 measures the cytotoxicity in Salmonella lux promotor cloned cells. They show that the Comet Assay or single cell gel ...
https://trevigene.com/products/401-trevigen/562-cometassay-electrokinetic-separation-starter-reagent-plus-power-supply-to-measure-eu-except-uk-switz
Comets are Threatening for Earth tooComets are Threatening for Earth too
Those who defend the planet ignore comets, and its due to wrong perception that comets cant be stopped. Its difficult to track the ice bodies as said by Nuth, but we can prevent it if we get prepared.. Get the Free Tracker App to find 2018 NES Classic in Stock. Comets are dangerous. The research reveals that its easy to deal with asteroids, because they are common. Its easy to detect NEAs, Near Earth Asteroids than comets, because NEAs are just like our planet. But, comets exist deep in the external solar system, so they are difficult to detect. According to scientists, the Halley comet revolves around sun once in every 75 years.. According to Nuth, comets suddenly appear from nowhere. He said that comet named Siding Spring appeared in 2013 and 2014 striking Mars, and both were dangerous. A space aircraft can destroy comet, but it takes 5 years to prepare a spacecraft, said by Nuth in AGU conference. Comet is larger than NEAs, so they strike the earth at high velocity.. On the contrary, NEA ...
https://www.i4u.com/2016/12/118688/comets-are-threatening-earth-too
Protection against DNA damage and inhibition of lipid peroxidation by flavones from Eremosparton songoricum (Litv) Vass » ...Protection against DNA damage and inhibition of lipid peroxidation by flavones from Eremosparton songoricum (Litv) Vass » ...
Seven flavonoids were isolated from Eremosparton songoricum (Litv) Vass and their antioxidation actions were examined. To investigate protective ability against DNA damage induced by H2O2, human peripheral blood mononuclear cells (PBMCs) were incubated with 5-hydroxy-7,4′-dimethoxyflavone,5,7-dihydroxy-3′,4′-dimethoxyflavone, acacetin, chrysoeriol, pedalitin, flavogadorinin and 5,7,4′-trihydroxy-3′-methoxyflavone-4′-O-D-glucoside separately and then treated by H2O2. The percentage of damaged cells were determined by single-cell gel electrophoresis. To examine their inhibition of lipid peroxidation, the compounds were incubated with rat microsomes and the reaction was initiated by Fe2+/Vc. The lipid peroxidation product thiobarbituric acid reactive substances (TBARS) was determined. The result showed most of flavonoids had concentration-dependent antioxdative activities. The relationship of antioxidation and the structure is discussed.
http://booksandjournals.brillonline.com/content/journals/10.1163/156856706775372799
PRIME PubMed | Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline...PRIME PubMed | Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline...
PubMed journal article: Validation of Drosophila melanogaster as an in vivo model for genotoxicity assessment using modified alkaline Comet assay. Download Prime PubMed App to iPhone, iPad, or Android
https://www.unboundmedicine.com/medline/citation/15899934/Validation_of_Drosophila_melanogaster_as_an_in_vivo_model_for_genotoxicity_assessment_using_modified_alkaline_Comet_assay_
Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis | July 7, 2016Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis | July 7, 2016
BioTek Note applicative, 07-Jul-16, Automated Comet Assay Imaging and Dual-Mask Analysis to Determine DNA Damage on an Individual Comet Basis
https://www.biotekinstruments.it/resources/application-notes/automated-comet-assay-imaging-and-dual-mask-analysis-to-determine-dna-damage-on-an-individual-comet-basis/
Astroblog: Comet C/2003 K2 (C/2008 X4) Christensen in CelestiaAstroblog: Comet C/2003 K2 (C/2008 X4) Christensen in Celestia
Just download these files into the extras folder of Celestia and you are ready to go (despite the comet only being magnitude 10, Celestia exagerates the comets tail and brightness, it does this for all comets around perihelion, but it makes them easy to find). You can get a whole passel of comet orbitals from the Celestia motherlode comet section, but be aware that the 164P-Christensen obits here are the old, pre-recovery orbits (also, if you load in the full catalog of comets, they sky will be taken up by comet orbits, best to use that as a resource to extract comets as you need them ...
http://astroblogger.blogspot.com/2008/12/comet-c2003-k2-c2008-x4-christensen-in.html
Comet assay - WikipediaComet assay - Wikipedia
The concept underlying the SCGE assay is that undamaged DNA retains a highly organized association with matrix proteins in the nucleus. When damaged, this organization is disrupted. The individual strands of DNA lose their compact structure and relax, expanding out of the cavity into the agarose. When the electric field is applied the DNA, which has an overall negative charge, is drawn towards the positively charged anode. Undamaged DNA strands are too large and do not leave the cavity, whereas the smaller the fragments, the farther they are free to move in a given period of time. Therefore, the amount of DNA that leaves the cavity is a measure of the amount of DNA damage in the cell. The image analysis measures the overall intensity of the fluorescence for the whole nucleoid and the fluorescence of the migrated DNA and compares the two signals. The stronger the signal from the migrated DNA the more damage there is present. The overall structure resembles a comet (hence comet assay) with a ...
https://en.wikipedia.org/wiki/Comet_assay
Safe Tech for Schools Maryland: March 2017Safe Tech for Schools Maryland: March 2017
The genotoxic effects of 2.45 GHz microwave (MW) radiation on the testis and ovary of Sprague Dawley rats was investigated. The animals were exposed to varying levels of specific absorption rate (SAR) of 0 (control), 0.48, 0.95, 1.43, 1.91, 2.39, 2.90, 3.40, 3.80 and 4.30 Wkg-1 , for 10 min. The induction of DNA damages was assessed using DNA direct amplification of length polymorphisms (DALP) and validated with single cell gel electrophoresis (SCGE) comet assay for same cells at SAR 2.39 Wkg-1 . Potential damage at the organ level was assessed by histopathological study. The results show significant differences in the Olive moment and % DNA in the blood of the exposed animals when compared with the control (p , 0.05). Hyperchromasia was observed in the ovary of the animals exposed to MW radiation. Also, there was reduction in the number of germ cells and cell disorganization in the testis of exposed group with increasing SARs. These results suggest that MW radiation has the potential to affect ...
http://safetechforschoolsmaryland.blogspot.ca/2017/03/
Safe Tech for Schools Maryland: March 2017Safe Tech for Schools Maryland: March 2017
The genotoxic effects of 2.45 GHz microwave (MW) radiation on the testis and ovary of Sprague Dawley rats was investigated. The animals were exposed to varying levels of specific absorption rate (SAR) of 0 (control), 0.48, 0.95, 1.43, 1.91, 2.39, 2.90, 3.40, 3.80 and 4.30 Wkg-1 , for 10 min. The induction of DNA damages was assessed using DNA direct amplification of length polymorphisms (DALP) and validated with single cell gel electrophoresis (SCGE) comet assay for same cells at SAR 2.39 Wkg-1 . Potential damage at the organ level was assessed by histopathological study. The results show significant differences in the Olive moment and % DNA in the blood of the exposed animals when compared with the control (p , 0.05). Hyperchromasia was observed in the ovary of the animals exposed to MW radiation. Also, there was reduction in the number of germ cells and cell disorganization in the testis of exposed group with increasing SARs. These results suggest that MW radiation has the potential to affect ...
http://safetechforschoolsmaryland.blogspot.com.au/2017/03/
Who are the CIOC Team? | Coordinated Investigations Of CometsWho are the CIOC Team? | Coordinated Investigations Of Comets
diversity among more than two dozen comets observed to date.. Tony Farnham (University of Maryland, College Park, Maryland) is an astronomer who studies the dynamics of cometary nuclei and the interrelationship between a comets nucleus and its coma. He was a member of the Science teams for the Deep Impact mission to comet Tempel 1, the extended mission (DIXI) to comet Hartley 2, and the Stardust-Next mission that returned to comet Tempel 1. He also led one of the teams that performed the analysis of the dust hazards posed by comet Siding Spring during its close approach to Mars.. Kelly Fast (NASA Headquarters) [Bio coming soon...] Yan Fernandez (University of Central Florida, Orlando, FL) is an astronomer who has been studying comets and asteroids since the mid-1990s. He has studied comets, asteroids, Trojans, Centaurs, and small moons at IR, visible, and radio wavelengths for over ten years with the goal of determining their physical and compositional properties. He is the PI of the Spitzer ...
http://cometcampaign.org/the-CIOC-team
NIOSHTIC-2 Publications Search - 20040849 - Detection of DNA damage in workers exposed to JP-8 jet fuel.NIOSHTIC-2 Publications Search - 20040849 - Detection of DNA damage in workers exposed to JP-8 jet fuel.
The genotoxicity of jet propulsion fuel 8 (JP-8) was assessed in the leukocytes of archived blood specimens from U.S. Air Force personnel using the comet assay. No differences in mean comet assay measurements were found between low, moderate, and high exposure groups before or after a 4 hour work shift. Before the work shift, mean tail DNA and mean tail (Olive) moment increased as the concentratio
https://www.cdc.gov/niosh/nioshtic-2/20040849.html
Hitchhikers Guide to the Galaxy on Comet
A comet is an icy small Solar System body that, when passing close to the Sun, warms and begins to release gases, a process called outgassing. This produces a visible atmosphere or coma, and sometimes also a tail. These phenomena are due to the effects of solar radiation and the solar wind acting upon the nucleus of the comet. Comet nuclei range from a few hundred metres to tens of kilometres across and are composed of loose collections of ice, dust, and small rocky particles. The coma may be up to 15 times the Earths diameter, while the tail may stretch one astronomical unit. If sufficiently bright, a comet may be seen from the Earth without the aid of a telescope and may subtend an arc of 30° (60 Moons) across the sky. Comets have been observed and recorded since ancient times by many cultures.. Comets usually have highly eccentric elliptical orbits, and they have a wide range of orbital periods, ranging from several years to potentially several millions of years. Short-period comets ...
http://hitchhikersgui.de/Comet
Plus itPlus it
879 Polycyclic aromatic hydrocarbons (PAHs) are environmental pollutants implicated in the causation of human lung cancer. One pathway of metabolic activation that yields reactive oxygen species (ROS) involves the oxidation of PAH trans-dihydrodiols by aldo-keto reductases (AKRs) to yield reactive and redox-active o-quinones, which can cause oxidative DNA damage (e.g. 8-oxo-dGuo). Evidence for this pathway in lung cells has been hampered by the difficulty in detecting 8-oxo-dGuo levels reliably in intact cells. We used the single cell gel electrophoresis (Comet) assay to detect DNA strand breaks in human lung adenocarcinoma (A549) cells (which express AKR1A1 and AKR1C isoforms) following treatment with BP-7,8-diol (AKR substrate), and BP-7,8-dione (AKR-product) and (+)-anti-BPDE. In the presence of DNA synthesis inhibitors (cytosine arabinoside and hydroxyurea) all three PAH metabolites produced significant DNA strand breaks in a concentration-dependent manner. Inclusion of ROS scavengers ...
http://cancerres.aacrjournals.org/content/67/9_Supplement/879
comet | HCS-pharmacomet | HCS-pharma
The comet assay is a sensitive, well established technique for quantifying DNA damage in eukaryotic cells. Compatible with the detection of a wide range of DNA damaging agents, its principle consists in migration of fragmented DNA in an electrophoresis gel (damaged DNA forming the tail of the comet), while intact DNA moves at a slower rate (head of the comet). The percentage of fragmented DNA in the comet tail is a direct measure of DNA damage.. ...
http://hcs-pharma.com/tag/comet/
Comet NEOWISE Over Californias North Coast - MindscapeFXComet NEOWISE Over Californias North Coast - MindscapeFX
Comet NEOWISE graces skies of far northern Californias Pacific and Redwood coasts, Humboldt County, California. What is a comet, and why do we see it? Comets are made of ice, rock and dust, like a dirty snowball. The ice may comprise any of a number of gases, frozen into a solid state in the icy blackness of space. As comets approach the sun, the suns energy acts on the comets surface, causing molecules of the ice to sublimate, or become gas again without going through a liquid state. As this happens, some larger particles of dust and debris making up the comet nucleus, or solid center, are blown outward into the surrounding space. This combination of gasses and larger particles surrounding the nucleus makes the hazy head, and forms the tail(s).
https://mindscapefx.com/2020/07/20/comet-neowise-over-the-north-coast/?replytocom=175
AstroDuet: The Cliffs of Comet Churyumov GerasimenkoAstroDuet: The Cliffs of Comet Churyumov Gerasimenko
These high cliffs occur on the surface of a comet. They were discovered to be part of the dark nucleus of Comet Churyumov-Gerasimenko (CG) by Rosetta, a robotic spacecraft launched by ESA which began orbiting the comet in early August. The ragged cliffs, as featured here, were imaged by Rosetta about two weeks ago. Although towering about one kilometer high, the low surface gravity of Comet CG would likely make a jump from the cliffs, by a human, survivable. At the foot of the cliffs is relatively smooth terrain dotted with boulders as large as 20 meters across. Data from Rosetta indicates that the ice in Comet CG has a significantly different deuterium fraction -- and hence likely a different origin -- than the water in Earths oceans. The Rosetta spacecraft is scheduled to continue to accompany the comet as it makes its closest approach to the Sun in 2015 August. via NASA http://ift.tt/ ...
http://astroduet.blogspot.com/2014/12/the-cliffs-of-comet-churyumov.html
JoVE Author Search: %C3%81d%C3%A1m B
Hydrogen, Lead, Ability, Alkaline Comet Assay, Alkylation, Alkylators, Blood, Cancer, Cell, Cells, Chromatography, Comet Assay, Concentration, Concentrations, DNA, DNA Damage, Epithelial Cells, Ethylene Oxide, Gas Chromatography, Human
http://labindex.jove.com/author/%C3%81d%C3%A1m-B
Comet Analysis Software - TrevigenComet Analysis Software - Trevigen
The software applies state of the image processing methods to automatically locate and score comets to characterize and quantify the degree of DNA damage revealed by the comet assay. It is compatible with 4X images which allows for rapid screening of up to 300 comets in seconds when analyzing CometChip®. Trevigens Comet Analysis Software can rapidly evaluate large numbers of cells, related to each treatment group or screening target in a study, and generate summary statistics based on the corresponding numeric results.. ...
https://trevigen.com/products-services/cell-stress-and-dna-damage/dna-damage/comet-assay/cell-stress-and-dna-damage-dna-damage-comet-assay-comet-analysis-software/comet-analysis-software/
Lokaverkefni: Greining DNA-skemmda af völdum PARP-hindra og vetnisperoxíðs með tvívíðum þáttháðum rafdrætti | SkemmanLokaverkefni: Greining DNA-skemmda af völdum PARP-hindra og vetnisperoxíðs með tvívíðum þáttháðum rafdrætti | Skemman
Two approaches were used to induce DNA strand breaks. On the one hand, FANCD1-/- fibroblasts were treated with a PARP inhibitor to inhibit DNA repair. For comparison, wild type cells were treated the same way. On the other hand, wild type fibroblasts were treated with hydrogen peroxide, which causes oxidative damage to DNA, including single- and double-stranded breaks. The latter samples were also analysed by the comet assay for comparison to 2D-SDE. The comet assay is the commonest detection method for DNA strand breaks and one of the objectives of the development of 2D-SDE is to design a method that is less time-consuming and easier to carry out than the comet assay ...
https://skemman.is/handle/1946/15775
Oxygen on Comet Not Created From SurfaceOxygen on Comet Not Created From Surface
Molecular oxygen is two oxygen atoms joined together, and on Earth it is essential for life, where it is produced by photosynthesis. It has been previously detected around some of the icy moons of Jupiter, but it was not expected to be found around a comet. The Rosetta science team originally reported that the oxygen was most likely from the comets main body, or nucleus. This meant it was primordial - that it was already present when the comet itself formed at the beginning of the Solar System 4.6 billion years ago. One group of outside researchers however suggested there might be a different source for molecular oxygen at comets. They had discovered a new way to produce molecular oxygen in space triggered by energetic ions-electrically charged molecules. They proposed that reactions with energetic ions on the surface of comet 67P could instead be the source of the detected molecular oxygen. Now, members of the Rosetta team have analysed the data on 67Ps oxygen in light of the new theory. In ...
https://www.infowars.com/oxygen-on-comet-not-created-from-surface/
SOHO Watches a Comet Fading AwaySOHO Watches a Comet Fading Away
The SWAN data captured another surprise. On Sept. 30, the hydrogen jumped by a factor of two and a half in a single day. It dropped down again some six weeks later.. Standard models of how comets behave helped Combis team correlate the hydrogen signature to just how much of the comets surface should be giving off water, a process known as sublimating, because the water turns directly from ice to a vapor without passing through a liquid phase. The amount of surface area predicted didnt jibe with what EPOXI itself saw - a comet that only gave off water from one half of its shape. But EPOXI also captured images of an extended halo of icy fragments that burst off the comet, most likely flung into space by carbon dioxide emissions on the comets surface. These ice chunks probably added sublimated water to the hydrogen cloud.. The rate of water generation being so much higher in 1997 implies that the ice fragmentation was even more severe then, says solar physicist Joe Gurman, U.S. project ...
http://www.innovations-report.com/html/reports/physics-astronomy/soho-watches-a-comet-fading-179438.html
Hydrogen isocyanide in comet 73P/Schwassmann-Wachmann (fragment B) - CaltechAUTHORSHydrogen isocyanide in comet 73P/Schwassmann-Wachmann (fragment B) - CaltechAUTHORS
We present a sensitive 3 sigma upper limit of 1.1% for the HNC/HCN abundance ratio in comet 73P/SchwassmannWachmann (fragment B), obtained on 2006 May 10-11, using Caltech Submillimeter Observatory (CSO). This limit is a factor of ~7 lower than the values measured previously in moderately active comets at 1 AU from the Sun. Comet 73P/Schwassmann-Wachmann was depleted in most volatile species, except of HCN. The low HNC/HCN ratio thus argues against HNC production from polymers produced from HCN. However, thermal degradation of macromolecules, or polymers, produced from ammonia and carbon compounds, such as acetylene, methane, or ethane appears a plausible explanation for the observed variations of the HNC/HCN ratio in moderately active comets, including the very low ratio in comet 73P/Schwassmann-Wachmann reported here. Similar polymers have been invoked previously to explain anomalous N-14/N-15 ratios measured in cometary CN. ...
https://authors.library.caltech.edu/12598/
Spatially resolved quantitative spectroscopy of comets - NASA/ADSSpatially resolved quantitative spectroscopy of comets - NASA/ADS
Because of their temporal and spatial variations, modern ground-based studies of comets within approximately 4 A.U. of the sun are observationally demanding tasks. Over the years, researchers have attempted resolved spectroscopy of comets covering a wide range of intrinsic luminosity. Recently this group has developed spectral and direct-imaging procedures to detect weak ion tails submerged into the comae of even fairly faint comets. Although the last year was devoid of any really bright comet, re-analysis of older dust and gas production data of the 1986 P/Halley apparition has been almost completed. The main changes are that the nucleus does make a significant contribution to continuum light, and that the gravity effect in the dust escape velocity is somewhat larger than previously assumed. On the direct observational side, spectral studies of the 1987s Comet Bradfield show it to be qualitatively carbon-rich, at least in the outer coma ratio of CO(+) and CO2(+), compared the H2O(+) with respect to
https://ui.adsabs.harvard.edu/abs/1988plas.rept..121S/abstract
The Nature and Origin of Comets and The Evolution of Celestial Bodies
7. The scientific papers and popularized book by S. V. M. Clube and W. M. Napier [Nature, 282 (29 November, 1979), P. 45S; Q. JI. Asir. Soc. (1982),23, pp. 45-66; and The Cosmic Serpent (London and New York, 1982)] deserve comment in light of the present paper. Their statement to the astronomical community that Velikovsky was at least close to correct and that comets (and in particular a body named Venus) did play important roles in early civilizations is long overdue. They also recognize the irrational behavior with which scientists confronted Velikovsky, and the overwhelming data pointing towards worldwide catastrophism caused by comets. I maintain, however, that Clube and Napiers findings support the present paper more precisely than their own theory of terrestrial catastrophism. Both papers agree that comets are of interstellar origin, and that the primordial Oort cloud concept does not explain the observed comet flux in the solar system. They support the ice ball comet model, ...
http://www.bibliotecapleyades.net/ciencia/ciencia_asteroids_comets28.htm
New evidence: Comets seeded life on Earth • The RegisterNew evidence: Comets seeded life on Earth • The Register
Ever since its formation, the Earth has been bombarded by comets, and scientists think they now have evidence that these cosmic missiles could have carried the building blocks for life along with them when they impacted.. In 2009, NASAs Stardust mission rendezvoused with the comet Wild-2 and returned to Earth with a sample of an amino acid the probe had picked up from comets outgassing. Tests showed it had too much carbon-13 to have come from Earth, but skepticism remains that simple amino acids could have kick-started the development of life on the planet.. To test that hypothesis, a team of scientists at the University of California, Berkeley, and the University of Hawaii, Manoa, built a pseudo-comet in the lab - a snowball of carbon dioxide, ammonia, and various hydrocarbons such as methane, ethane, and propane - then chilled it in an ultra-high-vacuum chamber down to 10 degrees above absolute zero.. The comet was then zapped with high-energy electrons to simulate the cosmic rays it would ...
http://www.theregister.co.uk/2013/03/06/comets_seed_life_experiment/
Testimonials - Red CometTestimonials - Red Comet
Our experience with Red Comet was very easy and smooth. We needed to use Red Comet during the pandemic to fulfill some of my childs requirements to graduate. The school district accepted the classes and she was off. She was able to pace herself and get the work done when she had time. She set a schedule for herself and met many of those timeline dates with the help of Red Comet organization structure. I would highly recommend Red Comet classes as a substantive learning tool for high school students. ...
https://www.redcomet.org/testimonials/
NYE: Night of the Comet? - GROIN
http://www.godlikeproductions.com/forum1/message3383800/pg1. People are talking about a large comet that is supposedly visible to the naked eye and will be passing on NYE. This is supposed to go down while partyers are out in the streets, waiting for the ball to drop. Some website forums are spelling doom for society and talking about elites taking to underground bunkers in anticipation of the comet slamming into Earth.. This all very closely resembles the plot of the classic 80s camp sci-fi classic Night of the Comet. 80s camp films have a lot of social criticisms embedded in them, which are poignant, and often overlooked. In that film, those who follow the crowd (and stand in the streets watching the comet) are turned into a pile of red dust. Only their sneakers and clothes remain. Just like holiday shoppers -even the survivors in the film would mostly rather go fashion shopping than worry about more practical type things. Meanwhile scientists are living in underground bunkers, aiming to ...
http://www.groin.com/nye-night-of-the-comet/
OSA | Removal of subsurface fluorescence in cryo-imaging using deconvolutionOSA | Removal of subsurface fluorescence in cryo-imaging using deconvolution
Reduction of comet tail artifacts using image restoration algorithms. Microspheres (15.4 μm diameter) were embedded in O.C.T phantom, imaged, and processed. MIPS of a sub volume are shown. The unprocessed image (a) shows the comet tail artifact extending to a depth of ≈100 μm. RL processing (b) removes comet tail artifacts and restores the shape of the bead both in-plane and along Z. Next-image processing (c) reduces the comet tail artifacts but in-plane spread persists. Wiener deconvolution (d) removes comet tail artifacts but the resultant image suffers from high frequency noise and in-plane restoration is not as effective as in RL. Parameters were: voxel size 4x4x5µm3, (RL, 20 iterations, damping factor of 3), and Wiener, NSR = 10−4. Scale bar corresponds to 50 µm ...
https://www.osapublishing.org/vjbo/abstract.cfm?URI=oe-18-21-22324
APOD Retrospective: March 13 - Starship Asterisk*APOD Retrospective: March 13 - Starship Asterisk*
The reaction of ancient peoples to the appearance of bright comets has toppled empires, de-throned kings, and been taken as a sign of great things to come. Probably some of these comets did not get as bright as Comet Hyakutake (hyah-koo-tah-kay) will in the next two weeks. It is likely that every major news organization will soon cover Comet Hyakutake extensively. Comet Hyakutake is shown above already showing a tail of dust. This image, taken directly from a photographic negative, shows stars as black spots and the bright comet coma and tail as dark clouds against the white background of space. During its closest approach to the Earth on March 25th, Comet Hyakutake will appear in the Northern hemisphere as a diffuse ball of light brighter than most stars. Comet Hyakutake will be visible then most of the night even without binoculars, passing above the stars in the handle of the Big Dipper. Comet Hyakutake will be best seen in dark skies far from city lights, where its tail - possibly ...
http://asterisk.apod.com/viewtopic.php?f=46&t=27751
Why am I seeing smeared or smiling DNA band(s) or discrepant DNA migration? | BiotiumWhy am I seeing smeared or smiling DNA band(s) or discrepant DNA migration? | Biotium
Smearing and smiling in GelRed™ or GelGreen™ precast gels most often caused by overloading of DNA. If you see band migration shifts or smearing and smiling, try reducing the amount of DNA loaded. The recommended loading amount for ladders and samples of known concentration is 50-200 ng/lane. For samples of unknown concentration, try loading one half or one third of the usual amount of DNA. This usually solves band migration problems.. Tip #2: Try the post-staining protocol. To avoid any interference the dye may have on DNA migration, we recommend using the post-staining protocol. If your application requires loading more than the recommended amount of DNA, use the post-staining protocol. While we recommend post-staining gels for 30 minutes, you may be able see bands in as little as five minutes, depending on how much DNA is present. Post-staining solutions can be reused. See the GelRed™ Product Information Sheet or GelGreen™ Product Information Sheet for detailed protocols.. Other tips ...
https://biotium.com/faqs/smeared_bands_dna_migration_issues/
Genome instabilityGenome instability
Møller, P (2005). "Genotoxicity of environmental agents assessed by the alkaline comet assay". Basic Clin Pharmacol Toxicol. 96 ...
https://en.wikipedia.org/wiki/Genome_instability
Ingredients of cosmeticsIngredients of cosmetics
February 2006). "A comet assay study reveals that aluminium induces DNA damage and inhibits the repair of radiation-induced ... Yiu G (December 2004). "Rapid Communications: Antiperspirant Induced DNA Damage in Canine Cells by Comet Assay". Toxicol. Mech ...
https://en.wikipedia.org/wiki/Ingredients_of_cosmetics
Sudan ISudan I
Toxicologic Pathology 1982, 10 (2), 71-78 Tsuda, S.; Matsusaka, N.; Madarame, H.; et al., The comet assay in eight mouse organs ... Repeated-dose liver and gastrointestinal tract micronucleus assays with CI Solvent Yellow 14 (Sudan I) using young adult rats. ...
https://en.wikipedia.org/wiki/Sudan_I
Diana Anderson (scientist)Diana Anderson (scientist)
... "comet assay", which allows for detection of DNA damage with extraordinary sensitivity. Healthy chromosomes do not present Comet ... She worked with Mojgan Najafzadeh on the development of the comet assay test. She worked with IMSTAR to develop an accurate ... Anderson joined the University of Bradford as Chair of Biomedical Research and continued to work on comet assays. She showed ... 2000). "Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing". Environmental and ...
https://en.wikipedia.org/wiki/Diana_Anderson_(scientist)
O-ToluidineO-Toluidine
"Comparative investigation of multiple organs of mice and rats in the comet assay". Mutat. Res. 517 (1-2): 53-75. doi:10.1016/ ...
https://en.wikipedia.org/wiki/O-Toluidine
GenotoxicityGenotoxicity
As a consequence, many sophisticated techniques including Ames Assay, in vitro and in vivo Toxicology Tests, and Comet Assay ... Comet assays are one of the most common tests for genotoxicity. The technique involves lysing cells using detergents and salts ... 2000). "Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing" (PDF). Environmental and ... To assay for genotoxic molecules, researchers assay for DNA damage in cells exposed to the toxic substrates. This DNA damage ...
https://en.wikipedia.org/wiki/Genotoxicity
DNA fragmentationDNA fragmentation
It can be measured by e.g. the Comet assay or by the TUNEL assay. Men with sperm motility defects often have high levels of ... Using bright-field microscopy, the SCD test appears to be more sensitive than the TUNEL assay. Its main units of measurement is ... comparison between sperm chromatin dispersion test and terminal uridine nick-end labeling assay". Fertil. Steril. 94 (3): 1027- ... and TUNEL assay are both effective in detecting sperm DNA damage. ...
https://en.wikipedia.org/wiki/DNA_fragmentation
Martin Brown (cancer biologist)Martin Brown (cancer biologist)
"DNA Damage Measured by the Comet Assay in Head and Neck Cancer Patients Treated with Tirapazamine1". Neoplasia.com. Retrieved ...
https://en.wikipedia.org/wiki/Martin_Brown_(cancer_biologist)
Water immersion objectiveWater immersion objective
Electrophoretic preparations used in the case of comet assay can benefit from the use of water objectives. The refractive index ...
https://en.wikipedia.org/wiki/Water_immersion_objective
TryptopholTryptophol
A Pilot Study with Alkaline Comet Assay". Archives of Industrial Hygiene and Toxicology. 62 (1): 41-49. doi:10.2478/10004-1254- ...
https://en.wikipedia.org/wiki/Tryptophol
MetolachlorMetolachlor
Comet) assay". Env. Mol. Mut. 29 (3): 277-288. doi:10.1002/(SICI)1098-2280(1997)29:3. 3.0.CO;2-9.{{cite journal}}: CS1 maint: ...
https://en.wikipedia.org/wiki/Metolachlor
In vitro fertilisationIn vitro fertilisation
Level of DNA fragmentation as measured, e.g. by Comet assay, advanced maternal age and semen quality. Women with ovary-specific ...
https://en.wikipedia.org/wiki/In_vitro_fertilisation
Acesulfame potassiumAcesulfame potassium
Determination of the effects of some artificial sweeteners on human peripheral lymphocytes using the comet assay. Journal of ...
https://en.wikipedia.org/wiki/Acesulfame_potassium
Platinum nanoparticlePlatinum nanoparticle
The damage to the DNA was measured for individual cells using single-gel electrophoresis via the comet assay. Researchers have ...
https://en.wikipedia.org/wiki/Platinum_nanoparticle
DNA damage theory of agingDNA damage theory of aging
Swain, U; Subba Rao, K (Aug 2011). "Study of DNA damage via the comet assay and base excision repair activities in rat brain ... Hashimoto, K; Takasaki, W; Sato, I; Tsuda, S (Aug 2007). "DNA damage measured by comet assay and 8-OH-dG formation related to ... Helbock, HJ; Beckman, KB; Shigenaga, MK (January 1998). "DNA oxidation matters: the HPLC-electrochemical detection assay of 8- ... "Gene-specific oxidative lesions in aged rat brain detected by polymerase chain reaction inhibition assay". Free Radic. Res. 41 ...
https://en.wikipedia.org/wiki/DNA_damage_theory_of_aging
Tobacco smokingTobacco smoking
... in human cells evaluated with the comet assay". Mutat. Res. 629 (1): 7-13. doi:10.1016/j.mrgentox.2006.12.007. PMID 17317274. ...
https://en.wikipedia.org/wiki/Tobacco_smoking
SmokingSmoking
... in human cells evaluated with the comet assay". Mutat. Res. 629 (1): 7-13. doi:10.1016/j.mrgentox.2006.12.007. PMID 17317274. ...
https://en.wikipedia.org/wiki/Smoking
CigaretteCigarette
... in human cells evaluated with the comet assay". Mutat. Res. 629 (1): 7-13. doi:10.1016/j.mrgentox.2006.12.007. PMID 17317274. ... Cellulose acetate is degraded within 2-3 weeks under aerobic assay systems of in vitro enrichment cultivation techniques and an ...
https://en.wikipedia.org/wiki/Cigarette
CryptolepineCryptolepine
"Effects of the anti-malarial compound cryptolepine and its analogues in human lymphocytes and sperm in the Comet assay". ...
https://en.wikipedia.org/wiki/Cryptolepine
Plant senescencePlant senescence
Koppen G, Verschaeve L (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in radicle ...
https://en.wikipedia.org/wiki/Plant_senescence
Evolutionary history of plantsEvolutionary history of plants
Koppen G, Verschaeve L (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in radicle ...
https://en.wikipedia.org/wiki/Evolutionary_history_of_plants
GerminationGermination
Koppen G, Verschaeve L (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in radicle ...
https://en.wikipedia.org/wiki/Germination
PlantPlant
Koppen, G.; Verschaeve, L. (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in ...
https://en.wikipedia.org/wiki/Plant
SeedSeed
Koppen G; Verschaeve L (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in radicle ...
https://en.wikipedia.org/wiki/Seed
Sperm motilitySperm motility
"Sperm DNA damage measured by the alkaline Comet assay as an independent predictor of male infertility and in vitro ...
https://en.wikipedia.org/wiki/Sperm_motility
Edward L. SchneiderEdward L. Schneider
N. P. Singh of the "comet" assay for single cell DNA damage. Schneider is the chairperson of the advisory committee to the Los ...
https://en.wikipedia.org/wiki/Edward_L._Schneider
NicotineNicotine
... causes DNA damage in several types of human cells as judged by assays for genotoxicity such as the comet assay, ... Nicotine derived genotoxic effects in human primary parotid gland cells as assessed in vitro by comet assay, cytokinesis-block ...
https://en.wikipedia.org/wiki/Nicotine
BotryosphaeranBotryosphaeran
The absence of mutagenicity and genotoxicity assessed by the micronucleus, Ames and MTT tests, and the Comet assay, established ... nor was it genotoxic as assessed by the Ames test and Comet assay. When administered orally to mice by gavage, botryosphaeran ... botryosphaeran was confirmed not to be mutagenic nor genotoxic by the micronucleus test and Comet assay procedures. ...
https://en.wikipedia.org/wiki/Botryosphaeran
List of MeSH codes (H01)List of MeSH codes (H01)
... comet assay MeSH H01.181.529.307.437.190 - capillary electrophoresis MeSH H01.181.529.307.437.200 - cellulose acetate ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(H01)
List of MeSH codes (E05)List of MeSH codes (E05)
... comet assay The list continues at List of MeSH codes (E06). (Wikipedia articles in need of updating from February 2020, All ... comet assay MeSH E05.393.560.598 - micronucleus tests MeSH E05.393.600.300 - dna footprinting MeSH E05.393.620.311 - ligase ... comet assay MeSH E05.196.401.190 - electrophoresis, capillary MeSH E05.196.401.190.500 - electrophoresis, microchip MeSH ... colony-forming units assay MeSH E05.200.500.383.910 - tumor stem cell assay MeSH E05.200.500.385 - cytogenetic analysis MeSH ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)
PlantPlant
Koppen, G.; Verschaeve, L. (2001). "The alkaline single-cell gel electrophoresis/comet assay: a way to study DNA repair in ...
https://en.m.wikipedia.org/wiki/Plants
Earliest known life formsEarliest known life forms
Earth remains the only place in the universe known to harbor life.[10][11] The origin of life on Earth was at least 3.77 billion years ago, possibly as early as 4.41 billion years ago.[2][4][5] The Earth's biosphere extends down to at least 19 km (12 mi) below the surface,[12][13][14][15] and up to at least 76 km (47 mi)[16] into the atmosphere,[17][18][19] and includes soil, hydrothermal vents, and rock.[20][21] Further, the biosphere has been found to extend at least 914.4 m (3,000 ft; 0.5682 mi) below the ice of Antarctica,[22][23][24] and includes the deepest parts of the ocean,[25][26][27] down to rocks kilometers below the sea floor.[26][28][29] In July 2020, marine biologists reported that aerobic microorganisms (mainly), in "quasi-suspended animation", were found in organically-poor sediments, up to 101.5 million years old, 76.2 m (250 ft) below the seafloor in the South Pacific Gyre (SPG) ("the deadest spot in the ocean"), and could be the longest-living life forms ever found.[30][31] ...
https://en.wikipedia.org/wiki/Earliest_known_life_forms
Howard V. HendrixHoward V. Hendrix
"Those Wandring Eyes of His': Watching Guyon Watch the Naked Damsels Wrestling." Assays VII. Ed. Peggy A. Knapp and Gary F. ... "A Day of the Comet," "Avatars" and "Hole in the Road" (short stories) in The Mystic Muse, Summer 1987, Fall 1987, Spring 1988, ...
https://en.wikipedia.org/wiki/Howard_V._Hendrix
BoksburgBoksburg
Killian advised Dr W.E. Bok, Secretary of State for the Transvaal Republic, of the results of the assay. The result was the ... When the gold reefs to the north of Boksburg Lake near Comet were found to continue southwards under Boksburg Lake, towards the ... Samples of the quartz were sent to Pretoria for assaying,which confirmed the presence of gold. ...
https://en.wikipedia.org/wiki/Boksburg
Sperm Chromatin Structure AssaySperm Chromatin Structure Assay
SCSA has numerous advantages when compared to other sperm DNA fragmentation (sDF) assays [TUNEL assay, COMET assay, and Sperm ... Notably, SCSA outcompetes other convention sperm DNA fragmentation (sDF) assays such as TUNEL and COMET in terms of efficiency ... Sperm Chromatin Structure Assay (SCSA) is a diagnostic approach that detects sperm abnormality with a large extent of DNA ... "Sperm Chromatin Structure Assay (SCSA) , Center for Women's Health , OHSU". www.ohsu.edu. Retrieved 2021-03-31. Evenson, Donald ...
https://en.wikipedia.org/wiki/Sperm_Chromatin_Structure_Assay
CI chondriteCI chondrite
Furthermore, there seems to exist a connection to comets: like the comets, CI chondrites accreted silicates, ice and other ... The element assay of CI meteorite is used as a geochemical standard, as it has "a remarkably close relationship" to the makeup ... Gounelle computes the prior orbit of Orgueil to be that of a short-period comet. This debate assumes the distinction between ... Orgueil, Alais, Ivuna and Tonk all assay to higher gas levels than typical meteorites- Revelstoke is too small for traditional ...
https://en.wikipedia.org/wiki/CI_chondrite
Index of branches of scienceIndex of branches of science
... art of assaying Dosiology - study of doses Dynamics - study of forces and their effects on motion Dysgenics - study of racial ... study of comets Computer science - study of processes that interact with data Conchology - study of shells Connectomics - study ...
https://en.wikipedia.org/wiki/Index_of_branches_of_science
Honus WagnerHonus Wagner
... for I don't know a ballplayer in either league who would assay less quit to the ton than Wagner ... This was the real tragedy ... which accompanied his throws to first like the tail of a comet. In 1898, Wagner won a distance contest in Louisville by ...
https://en.wikipedia.org/wiki/Honus_Wagner
StarfishStarfish
Single arms that regenerate a whole individual are called comet forms. The division of the starfish, either across its disc or ... Newton, L. C.; McKenzie, J. D. (1995). "Echinoderms and oil pollution: A potential stress assay using bacterial symbionts". ...
https://en.wikipedia.org/wiki/Starfish
William Lofland DudleyWilliam Lofland Dudley
"The Luminosity of Comets". Science. 32 (817): 286-288. 1910. Bibcode:1910Sci....32..286D. doi:10.1126/science.32.817.286. ISSN ... President Grover Cleveland appointed Dudley a member of the Assay Commission of 1887 to examine the weight and fineness of ...
https://en.wikipedia.org/wiki/William_Lofland_Dudley
ActinActin
L. monocytogenes and S. flexneri both generate a tail in the form of a "comet tail" that gives them mobility. Each species ... The DNase I inhibition assay, the only test which allows the quantification of the polymerized actin directly in biological ... Lambrechts A, Gevaert K, Cossart P, Vandekerckhove J, Van Troys M (May 2008). "Listeria comet tails: the actin-based motility ... exhibits small differences in the molecular polymerization mechanism of their "comet tails". Different displacement velocities ...
https://en.wikipedia.org/wiki/Actin
Semen qualitySemen quality
For example, the percentage of sperm with highly damaged DNA, comet extent, DNA break number, and other comet measures has been ... enzyme-linked immunosorbent assay, radiolabelled antiglobulin assay. One of the most informative and specific tests is ... after treatment with selective serotonin reuptake inhibitors detected using semen analysis and sperm chromatin structure assay ...
https://en.wikipedia.org/wiki/Semen_quality
Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicologyFluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology
While comet assay permits to measure direct DNA-strand breaking capacity of a tested agent MN test allows estimating the ... Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing and biomonitoring. ... Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing and biomonitoring. While comet assay permits ... FISH plus comet assay allows the recognition of targets of DNA damage and repairing directly. FISH combined with MN test is ...
https://pubmed.ncbi.nlm.nih.gov/20840797/
CometAssay Electrophoresis Starter Kit 4250-050-ESK: R&D SystemsCometAssay Electrophoresis Starter Kit 4250-050-ESK: R&D Systems
Learn how CometAssay Electrophoresis Starter Kit from R&D Systems DNA Damage and Repair Kits supports DNA damage research. Used ... What is the CometAssay?. The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for ... CometAssay Electrophoresis Starter Kit offers a complete standardized system for the comet assay which includes a 50 test ... CometAssay Electrophoresis Starter Kit Summary. Provides the reagents and instrumentation to standardize the Comet assay. ...
https://www.rndsystems.com/products/cometassay-electrophoresis-starter-kit_4250-050-esk
Susceptibility of primary cultures of human prostate epilthelial cells to chemical carcinogens as detected using the Comet...Susceptibility of primary cultures of human prostate epilthelial cells to chemical carcinogens as detected using the Comet...
... of primary cultures of human prostate epilthelial cells to chemical carcinogens as detected using the Comet assay. Mutagenesis ... of primary cultures of human prostate epilthelial cells to chemical carcinogens as detected using the Comet assay. ...
https://eprints.lancs.ac.uk/id/eprint/9223/
Cleaver Scientific Introduces High Throughput COMET Assay System | LabWrenchCleaver Scientific Introduces High Throughput COMET Assay System | LabWrench
LabWrench article Cleaver Scientific Introduces High Throughput COMET Assay System ... This would equate to a significant time-saving of up to 90% when handling 50 COMET Assay slides.. The COMET Assay is an ... This new high-throughput COMET Assay device will greatly increase the number of samples that can be analysed, decrease assay ... Recently the COMET Assay has been introduced into standard tests, used for genotoxicity, by the pharmaceutical industry and for ...
https://www.labwrench.com/article/41182/cleaver-scientific-introduces-high-throughput-comet-assay-system
Barnes et al. 2008, Reproducible Comet Assay of Amorphous Silica Nanoparticles Detects No Genotoxicity.Barnes et al. 2008, Reproducible Comet Assay of Amorphous Silica Nanoparticles Detects No Genotoxicity.
2008, Reproducible Comet Assay of Amorphous Silica Nanoparticles Detects No Genotoxicity.. a.ewd-ufaq-post-margin { font-size: ... Reproducible Comet Assay of Amorphous Silica Nanoparticles Detects No Genotoxicity. Nano Letters, 2008, 8 (9), pp 3069-3074. ...
https://glantreo.com/ufaqs/barnes-et-al-2008-reproducible-comet-assay-of-amorphous-silica-nanoparticles-detects-no-genotoxicity/
GENTAUR - Bioxys Comet Assay Tanks
Catalog > CSL > Comet Assay Tanks. Comet Assay Tank for 10 Slides CSL-COM10 Price:. 316.00. ...
https://genoprice.com/aspnet_client/acatalog/Catalog_Comet_Assay_Tanks_271.html
Research | International Comet Assay Working Group | ICAWGResearch | International Comet Assay Working Group | ICAWG
The ComNet (comet network) project on the comet assay in human population studies was launched during the International Comet ... The European Comet Assay Validation Group (ECVAG) was one of the main pillars of ECNIS2, the continutation of the initial ... Comet assay and cell array for fast and efficient genotoxicity testing - FP6 project ... hCOMET (COST Action CA15132) - The comet assay as a human biomonitoring tool - is supported by COST (European Cooperation in ...
https://www.icawg.com/research
Application of the comet assay in human biomonitoring - StaffApplication of the comet assay in human biomonitoring - Staff
Application of the comet assay in human biomonitoring: An hCOMET perspective. Research output: Contribution to journal › Review ... The comet assay is a well-accepted biomonitoring tool to examine the effect of dietary, lifestyle, environmental and ... Controlling for confounding in the comet assay requires a case-by-case approach. Inter-laboratory variation in levels of DNA ... Although each factor influences at least one comet assay endpoint, the collective evidence does not indicate single factors ...
https://publichealth.ku.dk/staff/?pure=en%2Fpublications%2Fapplication-of-the-comet-assay-in-human-biomonitoring
Occupational Monitoring: Hazardous Drug Exposures in Healthcare | NIOSH | CDCOccupational Monitoring: Hazardous Drug Exposures in Healthcare | NIOSH | CDC
These include alkaline elution, and more recently, the Comet assay. These assays have been used in vitro and in animal studies ... Kopjar N and Garaj-Vrhovac V. Application of the alkaline comet assay in human biomonitoring for genotoxicity: A study on ... Sakhvidi MJZ, Hajaghazadeh M, Mostaghaci M, Mehrparvar AH, Sakhvidi FZ and Naghshineh E. Applicability of the comet assay in ... Assessment of DNA damage in Japanese nurses handeling antineoplastic drugs by the comet assay. J Occup Health. 2008; 50:7-12. ...
https://www.cdc.gov/niosh/topics/hazdrug/monitoring.html
Background document: comet assay as a method for the assessing DNA damage in aquatic organisms
... dc.contributor.author. Lyons, B ... Background document: comet assay as a method for the assessing DNA damage in aquatic organisms. es_ES. ...
http://www.repositorio.ieo.es/e-ieo/handle/10508/3332?show=full
Assessment of DNA damage in welders using comet and micronucleus assays | AVESİSAssessment of DNA damage in welders using comet and micronucleus assays | AVESİS
... number of control subjects were evaluated for DNA damage in the whole blood and isolated lymphocytes using the comet assay. The ... genotoxic damage in buccal epithelial cells of subjects was determined by micronucleus (MN) assay. Metal(loids) such as Cr, Mn ...
https://avesis.bozok.edu.tr/yayin/0219f6c0-cfe5-46a1-8a1a-669ca41f6da3/assessment-of-dna-damage-in-welders-using-comet-and-micronucleus-assays
NIOSHTIC-2 Search Results - Full View
DeCS - Termos NovosDeCS - Termos Novos
COMET ASSAY ENSAYO COMETA ENTERÓCITOS ENTEROCYTES ENTEROCITOS ENXAQUECA CLÁSSICA CLASSIC MIGRAINE JAQUECA CLASICA ...
https://decs.bvs.br/P/news2000pie.htm
Modulation of RhoA GTPase Activity Sensitizes Human Cervix Carcinoma Cells to γ-Radiation by Attenuating DNA Repair PathwaysModulation of RhoA GTPase Activity Sensitizes Human Cervix Carcinoma Cells to γ-Radiation by Attenuating DNA Repair Pathways
Also, Chk1/Chk2 and histone H2A phosphorylation data, as well as comet assays, suggest that the levels of DNA damage and DNA ... Alkaline Comet Assay. The alkaline comet assay was performed as described by Singh et al., 1998 [12], with modifications. HeLa ... Also, Chk1/Chk2 and histone H2A phosphorylation data, as well as comet assays, suggest that the levels of DNA damage and DNA ... b) Estimates of DNA damage and repair efficiency (by olive tail moment, or OTM, measurements from comet assays) in HeLa cell ...
https://www.hindawi.com/journals/omcl/2016/6012642/
3-Methacryloxypropyltrimethoxysilane - Safety Data Sheet3-Methacryloxypropyltrimethoxysilane - Safety Data Sheet
Test Type: comet assay Species: Rat. Application Route: inhalation (dust/mist/fume) Method: OECD Test Guideline 489. Result: ...
https://www.chemicalbook.com/msds/3-methacryloxypropyltrimethoxysilane.htm
Assessment of DNA damage by comet assay and fast halo assay in buccal epithelial cells of Indian women chronically exposed to...Assessment of DNA damage by comet assay and fast halo assay in buccal epithelial cells of Indian women chronically exposed to...
Assessment of DNA damage by comet assay and fast halo assay in buccal epithelial cells of Indian women chronically exposed to ... Assessment of DNA damage by comet assay and fast halo assay in buccal epithelial cells of Indian women chronically exposed to ... DNA damage was evaluated by comet assay and fast halo assay (FHA). The concentrations of particulate matter with aerodynamic ... Compared with control, BEC of biomass users illustrated 2.6-times higher comet tail % DNA (32.2 versus 12.4, p < 0.001), ...
https://bulletin.chemwatch.net/technical/assessment-of-dna-damage-by-comet-assay-and-fast-halo-assay-in-buccal-epithelial-cells-of-indian-women-chronically-exposed-to-biomass-smoke
ANNATTO EXTRACTS (JECFA 52, 2004)ANNATTO EXTRACTS (JECFA 52, 2004)
Comet assay. Norbixin 98%. Up to 450 mol/l, 2 h. Negative. Kovary et al. (2001). ... Table 4. Results of assays for genotoxicity with annatto extracts. Endpoint Test object Annatto extract. Concentration Results ... as evaluated by the comet assay. Hypoglycaemia was induced in the mice as discussed in section 2.1.4 (Fernandes et al., 2002). ... Rec Assaya. B. subtilus. Food grade annatto. Up to 1 mg/ml. Negative. Haveland-Smith (1981). ...
https://inchem.org/documents/jecfa/jecmono/v52je03.htm
The Antioxidant and Free Radical Scavenging Activities of Chlorophylls and PheophytinsThe Antioxidant and Free Radical Scavenging Activities of Chlorophylls and Pheophytins
... we used comet assay to measure prevention of H2O2 DNA damage; we tested for quenching of 1,1-diphenyl-2-picrylhydrazyl (DPPH); ... in a chemical assay. All chlorophylls and pheophytins showed significant dose-dependent activity in the assays, with the ... we used comet assay to measure prevention of H2O2 DNA damage; we tested for quenching of 1,1-diphenyl-2-picrylhydrazyl (DPPH); ... in a chemical assay. All chlorophylls and pheophytins showed significant dose-dependent activity in the assays, with the ...
https://www.scirp.org/journal/PaperInformation.aspx?paperID=35234
Drug-Repositioning Screens Identify Triamterene as a Selective Drug for the Treatment of DNA Mismatch Repair Deficient Cells |...Drug-Repositioning Screens Identify Triamterene as a Selective Drug for the Treatment of DNA Mismatch Repair Deficient Cells |...
Detection of cellular DNA damage by Comet assay. A commercially available Comet assay kit from Cell Biolabs (STA-351) was used ... For assessment of the Comet assay, 50 comets were scored per condition and ImageJ was used to quantify the intensity and score ... Cellular DNA damage was visualised as a comet tail shape. Assays were performed in triplicate and bar chart shows the ... Cellular DNA damage was visualised as a comet tail shape. Assays were performed in triplicate and bar chart shows the ...
https://aacrjournals.org/clincancerres/article/23/11/2880/79917/Drug-Repositioning-Screens-Identify-Triamterene-as
Registration Dossier - ECHARegistration Dossier - ECHA
Comet assay.. Type of information:. experimental study. Adequacy of study:. weight of evidence. Reliability:. 2 (reliable with ...
https://echa.europa.eu/registration-dossier/-/registered-dossier/24217/7/7/3
Biblio | Page 3 | Linus Pauling Institute | Oregon State University
Comet Assay. Song Y, Chung CS, Bruno RS, Traber MG, Brown KH, King JC, Ho E. 2009. Dietary zinc restriction and repletion ...
https://lpi.oregonstate.edu/biblio?page=2&f%5Bauthor%5D=1376&o=asc&s=keyword
Nanomaterials | Free Full-Text | In Vitro Genotoxicity of Polystyrene Nanoparticles on the Human Fibroblast Hs27 Cell LineNanomaterials | Free Full-Text | In Vitro Genotoxicity of Polystyrene Nanoparticles on the Human Fibroblast Hs27 Cell Line
The genotoxic effects of the PNPs were evaluated by the cytokinesis-block micronucleus (CBMN) assay after exposure to PNPs. We ... Karlsson, H.L. The comet assay in nanotoxicology research. Anal. Bioanal. Chem. 2010, 398, 651-666. [Google Scholar] [CrossRef] ... The Comet Assay as a Tool to Detect the Genotoxic Potential of Nanomaterials ... Cory, A.H.; Owen, T.C.; Barltrop, J.A.; Cory, J.G. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays ...
https://www.mdpi.com/2079-4991/9/9/1299
Registration Dossier - ECHARegistration Dossier - ECHA
Type of assay:. mammalian comet assay. Species:. rat. Strain:. Sprague-Dawley. Details on species / strain selection:. The rat ... from OFA Sprague-Dawley male rats as investigated by the in vivo Comet assay.. Duodenum :. The value for the percentage of DNA ... Type of assay:. micronucleus assay. Species:. rat. Strain:. Sprague-Dawley. Sex:. male. Details on test animals or test system ... The search for potential genotoxic activity of TPS 32 was assessed using the in vivo comet assay in the liver, glandular ...
https://www.echa.europa.eu/web/guest/registration-dossier/-/registered-dossier/5188/7/7/1
IJMS | Free Full-Text | The Antioxidant from Ethanolic Extract of Rosa cymosa Fruits Activates Phosphatase and Tensin Homolog...IJMS | Free Full-Text | The Antioxidant from Ethanolic Extract of Rosa cymosa Fruits Activates Phosphatase and Tensin Homolog...
Neutral Comet Assay for the Detection of DNA Double-Strand Breaks (DSBs). The assay was carried out using a CometAssayTM Kit ( ... To examine whether the effect of RCE on DNA damage could be related to apoptosis induction, comet and Western blotting assays ... MTT Cell Proliferation Assay. Culture plates (96-well) were used in the MTT assay. The cells were seeded at 4 × 104 per well ... following the manufacturers protocol for the neutral comet assay. Briefly, cancer cells (2 × 105 cells/mL) were treated with ...
https://www.mdpi.com/1422-0067/20/8/1935/htm
School of Pharmacy and Life Sciences | Academic Schools | RGUSchool of Pharmacy and Life Sciences | Academic Schools | RGU
Molecular biology (PCR, RT-PCR, comet assay). *Biochemistry (Western and Northern blotting, GC-MS, LC-MS, HPLC and capillary ...
https://www.rgu.ac.uk/study/academic-schools/school-of-pharmacy-and-life-sciences
Atm Software Project Software - Free Download Atm Software ProjectAtm Software Project Software - Free Download Atm Software Project
CaspLab - Comet Assay Software Project Lab is a cross-platform image analysis software to measure level of DNA damage in SCGE ( ...
https://www.winsite.com/atm/atm+software+project/
Wolfram Library ArchiveWolfram Library Archive
Digital Image Processing: Comet Assay Analysis [in Demos]. *Discrete-Time Event History Analysis Using Segmented Hazards [in ...
https://library.wolfram.com/infocenter/BySubject/Science/Biology/?page=13&pages_count=50
Cell JournalCell Journal
The DNA induced damages in cells were compared using alkaline comet assay method and dosimetry was performed by TLD-100. The ... Radiation induced DNA damage was evaluated by alkaline comet assay. Samples of spheroid cells were treated by radiation from ( ... Enzyme linked immunosorbent assay (ELISA) was used for serum VEGF assay. VEGF, VEGFR1 and VEGFR2 gene expression was detected ... Then the DNA damage was evaluated using the alkaline comet assay method. The results showed that 2ME2 in combination with gamma ...
https://www.researchgate.net/journal/Cell-Journal-2228-5806
ElectrophoresisGenotoxicityNeutral comet assayMicronucleus testMicronucleiLymphocytesGenetic toxicologyMammalianBuccalToxicityAlkaline cometMouse lymphomaSlidesAntioxidantsTailAmesWidelyCellsSamplesDetectNuclearExtentHumanDetectionDataSystemTestResearchExposureControlsApplicationSensitiveFormationComparisonProjectFast
Electrophoresis12
  • The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. (rndsystems.com)
  • Why Use the CometAssay Electrophoresis Starter Kit? (rndsystems.com)
  • CometAssay Electrophoresis Starter Kit offers a complete standardized system for the comet assay which includes a 50 test sample Reagent Kit (25 x 2 well slides), Alkaline Control Cells, and the CometAssay Electrophoresis System II for consistent and reproducible results. (rndsystems.com)
  • Developed in collaboration with the Oxidative Stress Group at the University of Leicester (Leicester, UK), the unique design of the COMPAC-50 enables simultaneous electrophoresis of up to 50 COMET Assay slides saving considerable lab space and reducing slide handling time by up to two-thirds when compared to previous standard methods. (labwrench.com)
  • This decreases assay time by 60%, and offers users the benefit of an electrophoresis tank with a substantially smaller footprint, and more uniform orientation of gels during electrophoresis, than is currently commercially available. (labwrench.com)
  • CaspLab - Comet Assay Software Project Lab is a cross-platform image analysis software to measure level of DNA damage in SCGE (Single Cell Gel Electrophoresis) method called also comet assay. (winsite.com)
  • Lymphocytes were isolated from blood samples and DNA breaks were measured using single-cell microgel electrophoresis technique under alkaline conditions (comet assay). (northumbria.ac.uk)
  • Changes genotoxicity were analyzed by single-cell gel electrophoresis (comet assay). (biomedcentral.com)
  • We used single-cell gel electrophoresis (comet assay) to detect DNA-SSB in motor neurons. (elsevier.com)
  • Cell DNA fragments assume a "comet with tail" formation on electrophoresis and are detected with an image analysis system. (bvsalud.org)
  • In contrast, DNA of damaged cells is driven out of the nuclei upon electrophoresis, which leads to a comet-like image. (symmes.fr)
  • Single cell gel electrophoresis assay (comet assay) for evaluating nanoparticles-induced DNA damage in cells. (cdc.gov)
Genotoxicity5
  • Comet assay and micronucleus (MN) test are widely applied in genotoxicity testing and biomonitoring. (nih.gov)
  • Recently the COMET Assay has been introduced into standard tests, used for genotoxicity, by the pharmaceutical industry and for the regulation of chemicals within the European Commission's Registration, Evaluation and Authorisation of Chemicals Programme. (labwrench.com)
  • 2008, Reproducible Comet Assay of Amorphous Silica Nanoparticles Detects No Genotoxicity. (glantreo.com)
  • To assess immediate and time-delayed DNA damage in two cell lines exposed to low doses of ionizing radiation by using the comet assay and micronucleus test, and to compare these two techniques in the analysis of low-dose induced genotoxicity. (greenmedinfo.com)
  • The comet assay evaluated genotoxicity. (bvsalud.org)
Neutral comet assay1
  • Sperm DNA damage measurements, assessed with the neutral comet assay, included comet extent (CE), percentage of DNA in tail (Tail%) and tail distributed moment (TDM). (cdc.gov)
Micronucleus test1
Micronuclei3
  • DNA damage was assessed in all samples using the alkaline comet assay, and micronuclei were determined in erythrocytes. (emfacts.com)
  • Comet assay and BMCyt (micronuclei and nuclear buds) data revealed DNA damage in soybean workers. (naturalsociety.com)
  • The device is also used for the micronuclei assay (genomic damage). (symmes.fr)
Lymphocytes1
  • In the present study, 48 welders and an equal number of control subjects were evaluated for DNA damage in the whole blood and isolated lymphocytes using the comet assay. (bozok.edu.tr)
Genetic toxicology1
  • This paper reviews the relevant literature on advantages and limitations of Comet-FISH and MN-FISH assays application in genetic toxicology. (nih.gov)
Mammalian2
  • Limitations of the salmonella/mammalian microsome assay (Ames test) to determine occupational exposure to cytostatic drugs. (cdc.gov)
  • Polysulfides, di-tert-dodecyl was not mutagenic in an in vitro bacterial reverse mutation assay (similar to OECD TG 471) or in an in vitro mammalian chromosomal aberration assay (OECD TG 473) with or without metabolic activation. (europa.eu)
Buccal2
  • The genotoxic damage in buccal epithelial cells of subjects was determined by micronucleus (MN) assay. (bozok.edu.tr)
  • the comet assay in peripheral leukocytes and the buccal micronucleus (MN) cytome assay (BMCyt) in exfoliated buccal cells were used to assess the effects of exposures to pesticides in soybean farm workers from Espumoso. (naturalsociety.com)
Toxicity2
  • The toxicity assays, at cellular and molecular levels, did not show any detrimental effects of SPION. (dovepress.com)
  • In an in vivo model of motor neuron apoptosis, DNA-SSB accumulate slowly in avulsed motor neurons before apoptotic nuclear features emerge, and the comet fingerprint is similar to NO toxicity. (elsevier.com)
Alkaline comet1
  • Radiation induced DNA damage was evaluated by alkaline comet assay. (researchgate.net)
Mouse lymphoma2
  • In a mouse lymphoma gene mutation assay with L5178Y cells (OECD TG 46), a biologically significant mutagenic activity was observed either with or without metabolic activation. (europa.eu)
  • In vitro cytotoxic and non genotoxic effects of gutta-percha solvents on mouse lymphoma cells by single cell gel (comet) assay. (bvsalud.org)
Slides2
  • This would equate to a significant time-saving of up to 90% when handling 50 COMET Assay slides. (labwrench.com)
  • However, the sample throughput of the COMET Assay has traditionally been a limiting factor, typically limited to handling smaller numbers of slides, and a time-consuming procedure due to their individual manipulation. (labwrench.com)
Antioxidants1
  • All chlorophylls and pheophytins showed significant dose-dependent activity in the assays, with the pheophytins being the strongest antioxidants. (scirp.org)
Tail2
  • Evaluation of the DNA "comet" tail shape and migration pattern allows for assessment of DNA damage. (rndsystems.com)
  • Single cells were analyzed with "Tri Tek Comet Score version 1.5" software and DNA break was measured based on the percentage of tail DNA alone, or in the presence of H2O2 (25 μM) as a positive control. (northumbria.ac.uk)
Ames1
  • Concentrated urine from workers is usually tested with a bacterial mutagenicity assay (Ames test) that is sensitive to many of the antineoplastic agents and/or their metabolites and the results compared to a control population. (cdc.gov)
Widely1
  • The new methodology employed by the COMPAC-50 is likely to make a significant impact upon the growing applications of the COMET Assay and be widely adopted as it makes a cumbersome assay much more refined. (labwrench.com)
Cells11
  • Thus, combination of FISH with the comet assay or MN test proved to be promising techniques for evaluation of the distribution of DNA and chromosome damage in the entire genome of individual cells. (nih.gov)
  • The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. (rndsystems.com)
  • Each lot of the Alkaline Control Cells, reagents and CometSlides are tested and qualified for use in the CometAssay ES II. (rndsystems.com)
  • Susceptibility of primary cultures of human prostate epilthelial cells to chemical carcinogens as detected using the Comet assay. (lancs.ac.uk)
  • The comet assay is a well-accepted biomonitoring tool to examine the effect of dietary, lifestyle, environmental and occupational exposure on levels of DNA damage in human cells. (ku.dk)
  • We used a picogreen and colonogenic assay to appraise the DNA damage and cell death in a spheroid culture of GBM cells caused by iodine-131 (I-131) beta radiation in the presence of topotecan (TPT). (researchgate.net)
  • The numbers of cells that survived were compared with untreated cells using a colonogenic assay. (researchgate.net)
  • Damage of irradiated cells were evaluated quantitatively by the Fast Micromethod assay. (researchgate.net)
  • 0.05) in fluorescence intensity in irradiated cells compared to control cells as determined by the Fast Micromethod assay represented induced SSB and DSB damages in the DNA of irradiated cells. (researchgate.net)
  • CHO and MRC-5 cells were exposed to 50 milliSievert (mSv) of ionizing radiation and assayed immediately after irradiation and at 16 or 12 passages post-irradiation, respectively. (greenmedinfo.com)
  • In vitro cytotoxic effects of PM (3.12-200.0 µg/mL) were analyzed in human lung epithelial cells (A549) and macrophage cells (RAW 264.7) by MTT assay (3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide). (bvsalud.org)
Samples1
  • This new high-throughput COMET Assay device will greatly increase the number of samples that can be analysed, decrease assay time, reagent requirements and risk of damage/loss of fragile low melting point gels. (labwrench.com)
Detect1
  • However, relatively high doses are needed in order to detect an effect and, because the assays are nonspecific, confounding factors must be controlled for. (cdc.gov)
Nuclear1
  • In Arabidopsis, the heroes where going Jack Wilson is the comet-nuclear to prevent a built-in cell prison into offsprings into effectively done revenge are high and unrelenting. (oldandelegant.com)
Extent2
  • Inter-laboratory variation in levels of DNA damage and to some extent also reproducibility in biomonitoring studies are issues that have haunted the users of the comet assay for years. (ku.dk)
  • The Comet assay is a technique that estimates the extent of breakage in DNA. (symmes.fr)
Human5
  • hCOMET (COST Action CA15132) - The comet assay as a human biomonitoring tool - is supported by COST (European Cooperation in Science and Technology). (icawg.com)
  • The main aim of hCOMET COST Action is to collect the results of individual human population studies creating a unified database of comet assay data relating to human health and disease, promoting the consolidation of a network of researchers actively involved in collecting comet assay data on DNA damage (and DNA repair) in human populations. (icawg.com)
  • The ComNet (comet network) project on the comet assay in human population studies was launched during the International Comet Assay Workshop meeting in Kusadasi, Turkey (September 13-16, 2011). (icawg.com)
  • to define the relevance of DNA damage (as measured with the comet assay) for human health and disease. (icawg.com)
  • Simple Plex Human TNF RI/TNFRSF1A assay kit for use on Ella instrument. (rndsystems.com)
Detection1
  • Alkaline assay conditions facilitate sensitive detection of single-strand damage. (bvsalud.org)
Data1
  • Also, Chk1/Chk2 and histone H2A phosphorylation data, as well as comet assays, suggest that the levels of DNA damage and DNA repair activation and efficiency in HeLa cell lines are correlated with active RhoA. (hindawi.com)
System1
  • Cleaver Scientific has introduced the innovative new COMPAC-50 High Throughput COMET Assay system. (labwrench.com)
Test1
  • While comet assay permits to measure direct DNA-strand breaking capacity of a tested agent MN test allows estimating the induced amount of chromosome and/or genome mutations. (nih.gov)
Research1
  • The COMET Assay is an increasingly popular procedure for assessing DNA damage, both in academic and commercial research labs. (labwrench.com)
Exposure3
  • This review describes the effect of intrinsic (i.e. sex, age, tobacco smoking, occupational exposure and obesity) and extrinsic (season, environmental exposures, diet, physical activity and alcohol consumption) factors on the level of DNA damage measured by the standard or enzyme-modified comet assay. (ku.dk)
  • Conclusion: SM exposure of the veterans revealed DNA damage as judged by the comet assay. (northumbria.ac.uk)
  • Exposure of motor neurons to nitric oxide (NO) donors (sodium nitroprusside or NONOate), H 2 O 2 , or NO donor plus H 2 O 2 rapidly induces DNA-SSB and causes motor neuron degeneration, the occurrence of which is dose and time related, as represented by comet formation and cell loss. (elsevier.com)
Controls1
  • In comparison to controls, AM from asphalt fume-exposed rats showed significant DNA damage as demonstrated by the Comet assay. (cdc.gov)
Application1
  • FISH technique also permits to study comet and MN formation, necessary for correct application of these methods. (nih.gov)
Sensitive1
  • Comet and micronucleus assays are sensitive enough to assess this damage, although the former seems to be more efficient. (greenmedinfo.com)
Formation1
  • and, we tested their ability to prevent formation of thiobarbituric acid reactive substances (TBARS) during Cu-mediated peroxidation of low density lipoprotein (LDL) in a chemical assay. (scirp.org)
Comparison1
  • Krepinsky A, Bryant DW, Davison L, Heddle J, McCalla DR, Douglas G and Michalko K. Comparison of three assays for genetic effects of antineoplastic drugs on cancer patients and their nurses. (cdc.gov)
Project1
  • The European Comet Assay Validation Group (ECVAG) was one of the main pillars of ECNIS2, the continutation of the initial Environmental Cancer Risk, Nutrition and Individual Susceptibility ( ECNIS ) FP7 project. (icawg.com)
Fast1
  • DNA damage was evaluated by comet assay and fast halo assay (FHA). (chemwatch.net)
Modulation of RhoA GTPase Activity Sensitizes Human Cervix Carcinoma Cells to γ-Radiation by Attenuating DNA Repair Pathways
Modulation of RhoA GTPase Activity Sensitizes Human Cervix Carcinoma Cells to γ-Radiation by Attenuating DNA Repair Pathways (hindawi.com)
Immobilization of a Laccase/2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic Acid System to Layered Double Hydroxide/Alginate...
Immobilization of a Laccase/2,2'-azino-bis-(3-ethylbenzothiazoline)-6-sulfonic Acid System to Layered Double Hydroxide/Alginate... (hindawi.com)
Nanomaterials | Free Full-Text | In Vitro Genotoxicity of Polystyrene Nanoparticles on the Human Fibroblast Hs27 Cell Line
Nanomaterials | Free Full-Text | In Vitro Genotoxicity of Polystyrene Nanoparticles on the Human Fibroblast Hs27 Cell Line (mdpi.com)
School of Pharmacy and Life Sciences | Academic Schools | RGU
School of Pharmacy and Life Sciences | Academic Schools | RGU (rgu.ac.uk)
IJMS | May 2018 - Browse Articles
IJMS | May 2018 - Browse Articles (mdpi.com)
FARMACIA Y BIOQUIMICA - Research output - San Luis Gonzaga National University of Ica
FARMACIA Y BIOQUIMICA - Research output - San Luis Gonzaga National University of Ica (cris.unica.edu.pe)
Profile | Biosciences | University of Exeter
Profile | Biosciences | University of Exeter (biosciences.exeter.ac.uk)
Frontiers | Tumor Hypoxia Drives Genomic Instability
Frontiers | Tumor Hypoxia Drives Genomic Instability (frontiersin.org)
Mathematics, Computer Science and Statistics | Statistics | Collaborative Research Center 386
Mathematics, Computer Science and Statistics | Statistics | Collaborative Research Center 386 (epub.ub.uni-muenchen.de)
Find Research outputs - Heriot-Watt Research Portal
Find Research outputs - Heriot-Watt Research Portal (researchportal.hw.ac.uk)
Genetically Modified Children FREE VIEWING - LIMITED TIME
Genetically Modified Children FREE VIEWING - LIMITED TIME (greenmedinfo.com)
Deoxyribonucleic acid damage in Iranian veterans 25 years after wartime exposure to sulfur mustard<...
Deoxyribonucleic acid damage in Iranian veterans 25 years after wartime exposure to sulfur mustard<... (researchportal.northumbria.ac.uk)
Toxiska effekter av nanopartiklar med fokus på genomisk instabilitet | Karolinska Institutet
Toxiska effekter av nanopartiklar med fokus på genomisk instabilitet | Karolinska Institutet (ki.se)
Andrew KLIGERMAN | Research biologist | Ph.D. | United States Environmental Protection Agency | US EPA | Integrated Systems...
Andrew KLIGERMAN | Research biologist | Ph.D. | United States Environmental Protection Agency | US EPA | Integrated Systems... (researchgate.net)
Té negro: MedlinePlus suplementos
Té negro: MedlinePlus suplementos (medlineplus.gov)
Potential advantages of DNA methyltransferase 1 (DNMT1)-targeted inhibition for cancer therapy - Fingerprint - Mayo Clinic
Potential advantages of DNA methyltransferase 1 (DNMT1)-targeted inhibition for cancer therapy - Fingerprint - Mayo Clinic (mayoclinic.pure.elsevier.com)
PDF) The role of supplementary dietary antioxidants on immune response in puppies
PDF) The role of supplementary dietary antioxidants on immune response in puppies (researchgate.net)
GMOs and Health: The Scientific Basis for Serious Concern and
GMOs and Health: The Scientific Basis for Serious Concern and (greenmedinfo.com)
PDF) Natural compounds discovered in Helleborus sp. (Ranunculaceae) with important medical potential,
PDF) Natural compounds discovered in Helleborus sp. (Ranunculaceae) with important medical potential, (researchgate.net)
CESAM »
CESAM » (cesam.ua.pt)
Cleaver Scientific Introduces High Throughput COMET Assay System | LabWrench
Cleaver Scientific Introduces High Throughput COMET Assay System | LabWrench (labwrench.com)
PDF) The Photobiology of Lutein and Zeaxanthin in the Eye
PDF) The Photobiology of Lutein and Zeaxanthin in the Eye (researchgate.net)
Environmental Sanitation - Research Supported by FAPESP
Environmental Sanitation - Research Supported by FAPESP (bv.fapesp.br)
(naturalsociety.com)
Infertilidad masculina y fragmentación del ADN espermático: Un problema actual
Infertilidad masculina y fragmentación del ADN espermático: Un problema actual (medigraphic.com)
Harlan Pinto's Profile | Stanford Profiles
Harlan Pinto's Profile | Stanford Profiles (profiles.stanford.edu)
REFLEX - Gutachten und Endbericht - Elektrosmog-Forum des IZgMF
REFLEX - Gutachten und Endbericht - Elektrosmog-Forum des IZgMF (izgmf.de)
Magnar Bjørås - Institute of Clinical Medicine
Magnar Bjørås - Institute of Clinical Medicine (med.uio.no)