OrganismsChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesInformation ScienceHealth Care
Comamonas testosteroniComamonasGram-Negative Aerobic BacteriaBiodegradation, EnvironmentalGram-Negative Aerobic Rods and CocciOxygenases3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)PseudomonasDioxygenasesHydroxysteroid DehydrogenasesCatechol 2,3-DioxygenaseAlcohol OxidoreductasesSecosteroidsPhenolHydroxybenzoatesBenzenesulfonatesPQQ CofactorMolecular Sequence Data3-Hydroxysteroid DehydrogenasesSteroidsDelftia acidovoransSteroid IsomerasesSequence Analysis, DNAOxidoreductasesPhthalic AcidsUrocanate HydrataseGene Expression Regulation, BacterialTestosteroneGenes, Bacterial
Comamonas testosteroni
A species of gram-negative, aerobic rods formerly called Pseudomonas testosteroni. It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or maleate as carbon sources.
Comamonas
A genus of gram-negative, straight or slightly curved rods which are motile by polar flagella and which accumulate poly-beta-hydroxybutyrate within the cells.
Gram-Negative Aerobic Bacteria
A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.
Biodegradation, Environmental
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
Gram-Negative Aerobic Rods and Cocci
A group of gram-negative bacteria consisting of rod- and coccus-shaped cells. They are both aerobic (able to grow under an air atmosphere) and microaerophilic (grow better in low concentrations of oxygen) under nitrogen-fixing conditions but, when supplied with a source of fixed nitrogen, they grow as aerobes.
Oxygenases
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
3-alpha-Hydroxysteroid Dehydrogenase (B-Specific)
A 3-hydroxysteroid dehydrogenase which catalyzes the reversible reduction of the active androgen, DIHYDROTESTOSTERONE to 5 ALPHA-ANDROSTANE-3 ALPHA,17 BETA-DIOL. It also has activity towards other 3-alpha-hydroxysteroids and on 9-, 11- and 15- hydroxyprostaglandins. The enzyme is B-specific in reference to the orientation of reduced NAD or NADPH.
Pseudomonas
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
Dioxygenases
Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.
Hydroxysteroid Dehydrogenases
Enzymes of the oxidoreductase class that catalyze the dehydrogenation of hydroxysteroids. (From Enzyme Nomenclature, 1992) EC 1.1.-.
Catechol 2,3-Dioxygenase
Catalyzes the oxidation of catechol to 2-hydroxymuconate semialdehyde in the carbazole and BENZOATE degradation via HYDROXYLATION pathways. It also catalyzes the conversion of 3-methylcatechol to cis, cis-2-hydroxy-6-oxohept-2,4-dienoate in the TOLUENE and XYLENE degradation pathway. This enzyme was formerly characterized as EC 1.13.1.2.
Alcohol Oxidoreductases
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
Secosteroids
Steroids in which fission of one or more ring structures and concomitant addition of a hydrogen atom at each terminal group has occurred.
Phenol
An antiseptic and disinfectant aromatic alcohol.
Hydroxybenzoates
Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.
Benzenesulfonates
Organic salts and esters of benzenesulfonic acid.
PQQ Cofactor
A pyrrolo-quinoline having two adjacent keto-groups at the 4 and 5 positions and three acidic carboxyl groups. It is a coenzyme of some DEHYDROGENASES.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
3-Hydroxysteroid Dehydrogenases
Catalyze the oxidation of 3-hydroxysteroids to 3-ketosteroids.
Steroids
A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed)
Delftia acidovorans
A species of gram-negative rod-shaped bacteria found ubiquitously and formerly called Comamonas acidovorans and Pseudomonas acidovorans. It is the type species of the genus DELFTIA.
Steroid Isomerases
Enzymes that catalyze the transposition of double bond(s) in a steroid molecule. EC 5.3.3.
Sequence Analysis, DNA
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Oxidoreductases
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Phthalic Acids
A group of compounds that has the general structure of a dicarboxylic acid-substituted benzene ring. The ortho-isomer is used in dye manufacture. (Dorland, 28th ed)
Urocanate Hydratase
An enzyme that catalyzes the conversion of 4,5-dihydro-4-oxo-5-imidazolepropanoate to urocanate and water. EC 4.2.1.49.
Gene Expression Regulation, Bacterial
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Testosterone
A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.
Genes, Bacterial
The functional hereditary units of BACTERIA.

Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441. (1/50)

Comamonas testosteroni TA441 degrades 3-(3-hydroxyphenyl)propionate (3HPP) via the meta pathway. A gene cluster required for degradation of 3HPP was cloned from strain TA441 and sequenced. The genes encoding six catabolic enzymes, a flavin-type hydroxylase (mhpA), extradiol dioxygenase (mhpB), 2-keto-4-pentenoate hydratase (mhpD), acetaldehyde dehydrogenase (acylating) (mhpF), 4-hydroxy-2-ketovalerate aldolase (mhpE) and the meta cleavage compound hydrolase (mhpC), were found in this cluster, encoded in this order. mhpD and mhpF were separated by two genes, orf4 and orf5, which were not necessary for growth on 3HPP. The gene mhpR, encoding a putative transcriptional activator of the IcIR family, was located adjacent to mhpA in the opposite orientation. Disruption of the mhpB or mhpR genes affected growth on 3HPP or trans-3-hydroxycinnamate. The mhpB and mhpC gene products showed high specificity for 3-(2,3-dihydroxyphenyl)propionate (DHPP) and the meta cleavage compound produced from DHPP, respectively.  (+info)

Crystal structure of delta(5)-3-ketosteroid isomerase from Pseudomonas testosteroni in complex with equilenin settles the correct hydrogen bonding scheme for transition state stabilization. (2/50)

Delta(5)-3-Ketosteroid isomerase from Pseudomonas testosteroni has been intensively studied as a prototype to understand an enzyme-catalyzed allylic isomerization. Asp(38) (pK(a) approximately 4.7) was identified as the general base abstracting the steroid C4beta proton (pK(a) approximately 12.7) to form a dienolate intermediate. A key and common enigmatic issue involved in the proton abstraction is the question of how the energy required for the unfavorable proton transfer can be provided at the active site of the enzyme and/or how the thermodynamic barrier can be drastically reduced. Answering this question has been hindered by the existence of two differently proposed enzyme reaction mechanisms. The 2.26 A crystal structure of the enzyme in complex with a reaction intermediate analogue equilenin reveals clearly that both the Tyr(14) OH and Asp(99) COOH provide direct hydrogen bonds to the oxyanion of equilenin. The result negates the catalytic dyad mechanism in which Asp(99) donates the hydrogen bond to Tyr(14), which in turn is hydrogen bonded to the steroid. A theoretical calculation also favors the doubly hydrogen-bonded system over the dyad system. Proton nuclear magnetic resonance analyses of several mutant enzymes indicate that the Tyr(14) OH forms a low barrier hydrogen bond with the dienolic oxyanion of the intermediate.  (+info)

Bioaugmentation of activated sludge by an indigenous 3-chloroaniline-degrading Comamonas testosteroni strain, I2gfp. (3/50)

A strain identified as Comamonas testosteroni I2 was isolated from activated sludge and found to be able to mineralize 3-chloroaniline (3-CA). During the mineralization, a yellow intermediate accumulated temporarily, due to the distal meta-cleavage of chlorocatechol. This strain was tested for its ability to clean wastewater containing 3-CA upon inoculation into activated sludge. To monitor its survival, the strain was chromosomally marked with the gfp gene and designated I2gfp. After inoculation into a lab-scale semicontinuous activated-sludge (SCAS) system, the inoculated strain maintained itself in the sludge for at least 45 days and was present in the sludge flocs. After an initial adaptation period of 6 days, complete degradation of 3-CA was obtained during 2 weeks, while no degradation at all occurred in the noninoculated control reactor. Upon further operation of the SCAS system, only 50% 3-CA removal was observed. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes revealed a dynamic change in the microbial community structure of the activated sludge. The DGGE patterns of the noninoculated and the inoculated reactors evolved after 7 days to different clusters, which suggests an effect of strain inoculation on the microbial community structure. The results indicate that bioaugmentation, even with a strain originating from that ecosystem and able to effectively grow on a selective substrate, is not permanent and will probably require regular resupplementation.  (+info)

Arrangement and regulation of the genes for meta-pathway enzymes required for degradation of phenol in Comamonas testosteroni TA441. (4/50)

Comamonas testosteroni TA441 degrades phenol by a meta-cleavage pathway after the occurrence of a spontaneous mutation that derepresses the aphKLMNOPQB operon encoding phenol hydroxylase and catechol 2,3-dioxygenase, the enzymes for the initial two steps of the degradation pathway. A gene cluster, aphCEFGHJI, encoding the meta-pathway enzymes for degradation of 2-hydroxymuconic semialdehyde (HMS) to TCA cycle intermediates was found downstream of the aphK operon. The upstream operon and the downstream gene cluster were found to be separated by two open reading frames of unknown function and an oppositely oriented aphT gene, which is similar to regulatory genes for ortho-cleavage of catechol or chlorinated catechols. A promoter assay using an aphC::lacZ transcriptional fusion plasmid revealed that the aphC promoter activity is induced by both phenol and HMS. The phenol-dependent induction was mediated by AphR and the HMS-dependent induction was mediated by AphT. The aphC promoter in strain TA441 was not silenced, unlike the cases of the aphK and aphR promoters, and was highly induced by HMS.  (+info)

Regulation of the steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase gene in Comamonas testosteroni. (5/50)

The Comamonas testosteroni 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase gene (hsdA) codes for an adaptive enzyme in the degradation of steroid compounds. However, no information was available on the molecular regulation of steroid-inducible genes nor on the mechanism of steroid signaling in procaryotes. We, therefore, investigated the cis- and trans-acting elements of hsdA expression to infer the mechanism of its molecular regulation by steroids. The gene was localized on a 5.257-kilobase EcoRI fragment of C. testosteroni chromosomal DNA. The promoter was characterized, and the transcriptional start site was identified. Two palindromic operator domains were found upstream of hsdA. A new gene coding for a trans-acting negative regulator (repressor A, RepA) of hsdA expression was characterized. The specific interaction between RepA, testosterone, and the operator domain is demonstrated. From our results we conclude that hsdA is under negative transcriptional control by an adjacent gene product (RepA). Accordingly, induction of hsdA by steroids in fact is a derepression, where steroidal inducers bind to the repressor, thereby preventing its binding to the hsdA operator.  (+info)

Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for p-toluenesulfonate degradation in Comamonas testosteroni T-2. (6/50)

The catabolic IncP1beta plasmid pTSA from Comamonas testosteroni T-2 was mapped by subtractive analysis of restriction digests, by sequencing outwards from the tsa operon (toluenesulfonate degradation), and by generating overlapping, long-distance-PCR amplification products. The plasmid was estimated to comprise 72 +/- 4 kb. The tsa region was found to be a composite transposon flanked by two IS1071 elements. A cryptic tsa operon was also present in the tsa transposon. Those backbone genes and regions which we sequenced were in the same order as the corresponding genes in resistance plasmid R751, and identities of about 99% were observed. Enrichment cultures with samples from four continents were done to obtain organisms able to utilize p-toluenesulfonate as the sole source of carbon and energy for aerobic growth. Most (15) of the 16 cultures (13 of them isolates) were obtained from contaminated sites and were attributed to three metabolic groups, depending on their metabolism of p-toluenesulfonate. The largest group contained the tsa transposon, usually (six of seven isolates) with negligible differences in sequence from strain T-2.  (+info)

Endotoxic properties of lipid A from Comamonas testosteroni. (7/50)

The lipid A from Comamonas testosteroni has been isolated and its complete chemical structure determined [Iida, T., Haishima, Y., Tanaka, A., Nishijima, K., Saito, S. & Tanamoto, K. (1996). Eur J Biochem 237, 468-475]. In this work, the relationship between its chemical structure and biological activity was studied. The lipid A was highly homogeneous chemically and was characterized by the relatively short chain length (C(10)) of the 3-hydroxy fatty acid components directly bound to the glucosamine disaccharide backbone by either amide or ester linkages. The lipid A exhibited endotoxic activity in all of the assay systems tested (mitogenicity in mouse spleen cells; induction of tumour necrosis factor alpha release from both mouse peritoneal macrophages and mouse macrophage-like cell line J774-1, as well as from the human monocytic cell line THP-1; induction of nitric oxide release from J774-1 cells; Limulus gelation activity and lethal toxicity in galactosamine-sensitized mice) to the same extent as did 'Salmonella minnesota' lipid A or Escherichia coli LPS used as controls. The strong endotoxic activity of the C. testosteroni lipid A indicates that the composition of 3-hydroxydecanoic acid is not responsible for the low endotoxicity of the lipid A observed in members of the genus Rhodopseudomonas, as has previously been suggested. Furthermore, both the lack of a second acylation of the 3-hydroxy fatty acid attached at the 3' position, and the substitution of the hydroxyl group of the 3-hydroxy fatty acid attached at position 2, do not affect the manifestation of endotoxic activity or species specificity.  (+info)

PhcS represses gratuitous expression of phenol-metabolizing enzymes in Comamonas testosteroni R5. (8/50)

We identified an open reading frame, designated phcS, downstream of the transcriptional activator gene (phcR) for the expression of multicomponent phenol hydroxylase (mPH) in Comamonas testosteroni R5. The deduced product of phcS was homologous to AphS of C. testosteroni TA441, which belongs to the GntR family of transcriptional regulators. The transformation of Pseudomonas aeruginosa PAO1c (phenol negative, catechol positive) with pROR502 containing phcR and the mPH genes conferred the ability to grow on phenol, while transformation with pROR504 containing phcS, phcR, and mPH genes did not confer this ability. The disruption of phcS in strain R5 had no effect on its phenol-oxygenating activity in a chemostat culture with phenol. The phenol-oxygenating activity was not expressed in strain R5 grown in a chemostat with acetate. In contrast, the phenol-oxygenating activity in the strain with a knockout phcS gene when grown in a chemostat with acetate as the limiting growth factor was 66% of that obtained in phenol-grown cells of the strain with a knockout in the phcS gene. The disruption of phcS and/or phcR and the complementation in trans of these defects confirm that PhcS is a trans-acting repressor and that the unfavorable expression of mPH in the phcS knockout cells grown on acetate requires PhcR. These results show that the PhcS protein repressed the gratuitous expression of phenol-metabolizing enzymes in the absence of the genuine substrate and that strain R5 acted by an unknown mechanism in which the PhcS-mediated repression was overcome in the presence of the pathway substrate.  (+info)

Comamonas testosteroni (Talalay) Tamaoka et al. emend. Willems et al.Comamonas testosteroni (Talalay) Tamaoka et al. emend. Willems et al.
Comamonas testosteroni ATCC ® 700441D-5™ Designation: Genomic DNA from Comamonas testosteroni strain JS42 TypeStrain=False Application:
https://www.atcc.org/en/Global/Products/A/4/3/3/700441D-5.aspx
Assimilation of aromatic compounds by Comamonas testosteroni: characterization and spreadability of protocatechuate 4,5...Assimilation of aromatic compounds by Comamonas testosteroni: characterization and spreadability of protocatechuate 4,5...
Comamonas testosteroni strain CNB-1 was isolated from activated sludge and has been investigated for its ability to degrade 4-chloronitrobenzene. Results from this study showed that strain CNB-1 grew on phenol, gentisate, vanillate, 3-hydroxybenzoate (3HB), and 4-hydroxybenzoate (4HB) as carbon and …
https://pubmed.ncbi.nlm.nih.gov/22996279/
Selenite reduction by the obligate aerobic bacterium Comamonas testosteroni S44 isolated from a metal-contaminated soil | BMC...Selenite reduction by the obligate aerobic bacterium Comamonas testosteroni S44 isolated from a metal-contaminated soil | BMC...
Selenium (Se) is an essential trace element in most organisms but has to be carefully handled since there is a thin line between beneficial and toxic concentrations. Many bacteria have the ability to reduce selenite (Se(IV)) and (or) selenate (Se(VI)) to red elemental selenium that is less toxic. A strictly aerobic bacterium, Comamonas testosteroni S44, previously isolated from metal(loid)-contaminated soil in southern China, reduced Se(IV) to red selenium nanoparticles (SeNPs) with sizes ranging from 100 to 200 nm. Both energy dispersive X-ray Spectroscopy (EDX or EDS) and EDS Elemental Mapping showed no element Se and SeNPs were produced inside cells whereas Se(IV) was reduced to red-colored selenium in the cytoplasmic fraction in presence of NADPH. Tungstate inhibited Se(VI) but not Se(IV) reduction, indicating the Se(IV)-reducing determinant does not contain molybdenum as co-factor. Strain S44 was resistant to multiple heavy and transition metal(loid)s such as Se(IV), As(III), Cu(II), and Cd(II)
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/s12866-014-0204-8
The conserved cis-Pro<sup>39</sup> residue plays a crucial role in the proper positioning of the catalytic base Asp<sup>38</sup...The conserved cis-Pro<sup>39</sup> residue plays a crucial role in the proper positioning of the catalytic base Asp<sup>38</sup...
TY - JOUR. T1 - The conserved cis-Pro39 residue plays a crucial role in the proper positioning of the catalytic base Asp38 in ketosteroid isomerase from Comamonas testosteroni. AU - Nam, Gyu Hyun. AU - Cha, Sun Shin. AU - Yun, Young Sung. AU - Yun Hee, O. H.. AU - Hong, Bee Hak. AU - Lee, Heung Soo. AU - Choi, Kwan Yong. PY - 2003/10/15. Y1 - 2003/10/15. N2 - KSI (ketosteroid isomerase) from Comamonas testosteroni is a homodimeric enzyme that catalyses the allylic isomerization of Δ5-3- ketosteroids to their conjugated Δ4-isomers at a reaction rate equivalent to the diffusion-controlled limit. Based on the structural analysis of KSI at a high resolution, the conserved cis-Pro39 residue was proposed to be involved in the proper positioning of Asp38, a critical catalytic residue, since the residue was found not only to be structurally associated with Asp38, but also to confer a structural rigidity on the local active-site geometry consisting of Asp38, Pro39, Val40, Gly41 and Ser42 at the ...
https://experts.umn.edu/en/publications/the-conserved-cis-prosup39sup-residue-plays-a-crucial-role-in-the
KEGG PATHWAY: Glycolysis / Gluconeogenesis - Comamonas testosteroni CNB-2
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
http://www.genome.jp/kegg-bin/show_pathway?ctt00010+CtCNB1_2792
KEGG PATHWAY: Phosphotransferase system (PTS) - Comamonas testosteroni CNB-2
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the ...
http://www.genome.jp/kegg-bin/show_pathway?ctt02060
CAS 68081-81-2 Sodium (C10-16)alkylbenzenesulfonate Chemical World ReportCAS 68081-81-2 Sodium (C10-16)alkylbenzenesulfonate Chemical World Report
CAS 68081-81-2 Sodium (C10-16)alkylbenzenesulfonate Chemical World Report This CAS 68081-81-2 - Sodium (C10-16)alkylbenzenesulfonate Chemical World...
https://www.researchandmarkets.com/reports/3911500/cas-68081-81-2-sodium-c10
Active site directed mutagenesis of 3 beta/17 beta-hydroxysteroid dehydrogenase establishes differential effects on short-chain...
Mutagenetic replacements of conserved residues within the active site of the short-chain dehydrogenase/reductase (SDR) superfamily were studied using prokaryotic 3 beta/17 beta-hydroxysteroid dehydrogenase (3 beta/17 beta-HSD) from Comamonas testosteroni as a model system. The results provide novel data to establish Ser 138 as a member of a catalytically important triad of residues also involving Tyr151 and Lys155. A Ser--|Ala exchange at position 138 results in an almost complete (| 99.9%) loss of enzymatic activity, which is not observed with a Ser--|Thr replacement. This indicates that an essential factor for catalysis is the ability of side chain 138 to form hydrogen bond interactions. Mutations in the NAD(H) binding region, in strands beta A, beta D, and adjacent turns, reveal two additional residues, Thr12 and Asn87, which are important for correct binding of the coenzyme and with a differential effect on the reactions catalyzed. Thus, mutation of Thr12 to Ala results in a complete loss of the 3
https://www.ndorms.ox.ac.uk/publications/108792
46074 PHCS Network Rehab - Private HealthCare Systems Treatment Centers 46074 - PHCS Network Treatment Programs 4607446074 PHCS Network Rehab - Private HealthCare Systems Treatment Centers 46074 - PHCS Network Treatment Programs 46074
Find PHCS Network Treatment Centers near 46074, get help from 46074 PHCS Network Rehab for PHCS Network Treatment near 46074, get help with Private HealthCare Systems near 46074.
https://www.psychologytoday.com/us/treatment-rehab/phcs/46074?sid=6081d8daa33de&zipdist=5&spec=208&spec=211&spec=224&spec=227&spec=240&spec=241&spec=242&spec=285
46074 PHCS Network Rehab - Private HealthCare Systems Treatment Centers 46074 - PHCS Network Treatment Programs 4607446074 PHCS Network Rehab - Private HealthCare Systems Treatment Centers 46074 - PHCS Network Treatment Programs 46074
Find PHCS Network Treatment Centers near 46074, get help from 46074 PHCS Network Rehab for PHCS Network Treatment near 46074, get help with Private HealthCare Systems near 46074.
https://www.psychologytoday.com/us/treatment-rehab/phcs/46074?sid=607ff323d9c25&cost=3&spec=229&spec=231&spec=242
Mutagenetix > Incidental...Mutagenetix > Incidental...
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq, Jul 2008 ...
https://mutagenetix.utsouthwestern.edu/incidental/incidental_rec.cfm?mid=634513
Structure of bacterial 3 beta/17 beta-hydroxysteroid dehydrogenase at 1.2 angstrom resolution : A model for multiple steroid...Structure of bacterial 3 beta/17 beta-hydroxysteroid dehydrogenase at 1.2 angstrom resolution : A model for multiple steroid...
The enzyme 3beta/17beta-hydroxysteroid dehydrogenase (3beta/17beta-HSD) is a steroid-inducible component of the Gram-negative bacterium Conramonas testosteroni. It catalyzes the reversible reduction/ dehydrogenation of the oxo/beta-hydroxy groups at positions 3 and 17 of steroid compounds, including hormones and isobile acids. Crystallographic analysis at 1.2 Angstrom resolution reveals the enzyme to have nearly identical subunits that form a tetramer with 222 symmetry. This is one of the largest oligomeric structures refined at this resolution. The subunit consists of a monomer with a single-domain structure built around a seven-stranded beta-sheet flanked by six alpha-helices. The active site contains a Ser-Tyr-Lys triad, typical for short-chain dehydrogenases/reductases (SDR). Despite their highly diverse substrate specificities, SDR members show a close to identical folding pattern architectures and a common catalytic mechanism. In contrast to other SDR apostructures determined, the ...
http://sh.diva-portal.org/smash/record.jsf?faces-redirect=true&language=no&searchType=SIMPLE&query=&af=%5B%5D&aq=%5B%5B%5D%5D&aq2=%5B%5B%5D%5D&aqe=%5B%5D&pid=diva2%3A508297&noOfRows=50&sortOrder=author_sort_asc&onlyFullText=false&sf=all
Forced Vaccinations Bill Passes MA Senate! - Nature Moms Blog » Nature MomsForced Vaccinations Bill Passes MA Senate! - Nature Moms Blog » Nature Moms
Elisa, dont come here and spout your opinion as fact. You will be denied because you are WRONG. You have been listening to Glenn Beck and Rush too long.. Check out this well researched wiki page on czars:. http://en.wikipedia.org/wiki/List_of_U.S._executive_branch_czars. It LISTS all the czars including ones appointed by Roosevelt. Hmmm… strange since you just claimed he never had any. Also Nixon, Lyndon Johnson, Clinton, Bush, and Obama. This page says Bush had 31 czars and 46 different people in the czar position. So perhaps my number of 36 IS off but it isnt even in the same ballpark as your figure… 12.. Your lowball figures are coming from conservative media who are nitpicking each and every czar under Bush and finding some teeny reason to say he was not REALLY a czar. They/you are seeing what you want to see.. But let us not forget that czars are essentially advisors. Czar is a media coined term. Can the President have too many advisors? Frankly I would HOPE a president would have a ...
http://naturemoms.com/blog/2009/09/03/forced-vaccinations-bill-passes-ma-senate/
Ford gets a product czar, tooFord gets a product czar, too
Ford will be following GM’s move of having a single person behind global product development, reports the Detroit News. The job of a “product czar” is to ensure that models are created as quickly and efficiently as possible as well as picking up on global car trends. GM’s Bob Lutz has been the product czar since 2001, and last year...
https://www.motorauthority.com/news/1028246_ford-gets-a-product-czar-too
White House Czar Calls for End to War on Drugs | Grasscity Forums - The #1 Marijuana Community OnlineWhite House Czar Calls for End to War on Drugs | Grasscity Forums - The #1 Marijuana Community Online
White House Czar Calls for End to War on Drugs - WSJ.com WASHINGTON -- The Obama administrations new drug czar says he wants to banish the idea...
https://forum.grasscity.com/threads/white-house-czar-calls-for-end-to-war-on-drugs.390366/
A Mysterious Killer: The Prion - Outlet
Trigger Warning: There are pictures in this article that may be upsetting to some. Viewer discretion is advised. Ello! This is the start of a new series that deal with mysterious diseases and unusual pathogens. The first of this showcase is the prion, a silent, mysterious and nigh-invulnerable killer with a 100% mortality rate. Lets start…
https://doubtandreason.wordpress.com/2017/04/12/a-mysterious-killer-the-prion/
Trump Covid Vaccine Czar Says Extremely Low Chance Pfizer and Moderna Shots Wont Work Against New Strain - NBC New YorkTrump Covid Vaccine Czar Says Extremely Low Chance Pfizer and Moderna Shots Wont Work Against New Strain - NBC New York
President Trumps coronavirus vaccine czar said Monday he expects Pfizers and Modernas Covid-19 shots will be effective against a new mutation of the...
https://www.nbcnewyork.com/news/business/money-report/trump-covid-vaccine-czar-says-extremely-low-chance-pfizer-and-moderna-shots-wont-work-against-new-strain/2793569/?amp
Camera Lomo SP400CZ La Sardina Camera & Flash Czar | PhotoSynthesisCamera Lomo SP400CZ La Sardina Camera & Flash Czar | PhotoSynthesis
Camera Lomo SP400CZ La Sardina Camera & Flash Czar on the price of 298lv. Specialized store with excellent prices and consultation. Free
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TSAR 005064 - Reverse transcriptase domain-containing protein - Trichomalopsis sarcophagae - TSAR 005064 gene & proteinTSAR 005064 - Reverse transcriptase domain-containing protein - Trichomalopsis sarcophagae - TSAR 005064 gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
https://www.uniprot.org/uniprot/A0A232ENU1
TSAR, Vol.1, No.10TSAR, Vol.1, No.10
TSAR is a free, digitally delivered art information utility. Every entry is carefully checked with the presenting institutions. Nevertheless, inaccuracies occur. Always try to call the sponsoring institution to check the facts. In our effort to be an increasingly more valuable product, we ask for your comments and suggestions to be emailed to [email protected] ...
http://www.eyewithwings.net/VanishingPoints/TSAR.htm
TO KILL A TSAR
Because Williams book follows closely historical events, anyone with a grasp of Russian history will know the outcome (though perhaps after Tarantinos Inglourious Basterds all bets are off when it comes to assassination attempts on real-life figures). It also means Hadfield is ultimately a rather ineffectual hero, flowing along with events with little power or opportunity to alter the fate of the Tsar or those who plan to kill him. ...
http://shotsmag.co.uk/book_reviews_view.aspx?book_review_id=49
Drug Czar Ramstad? | Drugs-ForumDrug Czar Ramstad? | Drugs-Forum
Drug Czar Ramstad? Now that the State and Treasury department picks have been officially unveiled, the parlor game of Obama cabinet speculation turns to the less high-profile posts - the people...
https://drugs-forum.com/ams/drug-czar-ramstad.6794/
Cyber-Security Czar To Be Named - SlashdotCyber-Security Czar To Be Named - Slashdot
The Washington Post and everybody else is reporting that on Tuesday President Obama will name Howard A. Schmidt as cyber-security czar. Schmidt was an advisor to President Bush on cyber-security matters. The Post rehearses the reasons why the Obama administration has had difficulty in finding someon...
https://politics.slashdot.org/story/09/12/22/034239/cyber-security-czar-to-be-named?sdsrc=prevbtmprev
New Federal Report Contradicts Drug Czar | Drugs-ForumNew Federal Report Contradicts Drug Czar | Drugs-Forum
August 26, 2004 - Washington, DC, USA Washington, DC: A newly released federal report refutes claims by US Drug Czar John Walters that the United States is being inundated with Canadian pot,...
https://drugs-forum.com/ams/new-federal-report-contradicts-drug-czar.2558/
WindyCitys Content - FootballsFuture.comWindyCitys Content - FootballsFuture.com
Our cap situation isnt good. We have about 40 million in cap space and can clear another 10 pretty easily. We also have 40 million in dead money, which is epicly bad. We will split it 25/15 into 2023 and further waste resources in Fields rookie deal. We also only have 28 players under contract and only 2 draft picks that will count towards the 51, which means that the 40 million will have to sign 23 guys. We will be functional, but not big stacks at the table in reality. We likely need to sign a ton of teir 2 and 3 guys and continue to backload them deals ...
https://forums.footballsfuture.com/profile/532-windycity/content/
Obamas Green Czar Explains Where the Jobs AreObamas Green Czar Explains Where the Jobs Are
Washington is spending $60 billion to create the careers of the future, but not a single green job yet exists. Obamas green czar explains.
http://www.newsweek.com/obamas-green-czar-explains-where-jobs-are-81655
TASS: Society & Culture - Remains of Tsars family unlikely to be lost completely, expert hopesTASS: Society & Culture - Remains of Tsars family unlikely to be lost completely, expert hopes
The Russian Orthodox Church posed a number of questions to the investigators and the state commission, which have remained unanswered
http://tass.com/society/977614
Schools Week: Sir Kevan Collins appointed education recovery tsar - Parents United
Downing Street said Collins would oversee a comprehensive programme of catch-up aimed at young people who have lost out on learning due to the pandemic.Sir Kevan Collins has been appointed as the governments education recovery commissioner. ...
https://www.parentsunited.net/schools-week-sir-kevan-collins-appointed-education-recovery-tsar/
Heard immunity | Philstar.comHeard immunity | Philstar.com
I heard from General Carlito Galvez, chief implementor of COVID plans and vaccine czar, that the bulk of COVID-19 vaccines will arrive in the second quarter this year.
https://www.philstar.com/business/2021/03/17/2084758/heard-immunity
Ins and Outs | MedgadgetIns and Outs | Medgadget
Why Is Wal-Mart Endorsing Employer Mandated Healthcare?... Tylenol Tsar FDA Panel Recommends Ban on Vicodin, Percocet... Candidates Aplenty for Spending on
https://www.medgadget.com/2009/07/ins_and_outs_167.html
MicroClear 108
Environmental Engineering and Consulting Company that provides onsite training, consulting, Bioaugmentation products, Lab services and Training materials
https://environmentalleverage.com/microclear108.htm
Mesoporous silica nanoparticles incorporating squaraine-based photosensitizers: a combined experimental and computational...Mesoporous silica nanoparticles incorporating squaraine-based photosensitizers: a combined experimental and computational...
Squaraine dyes, that exhibit intense absorption in the red/near infrared region, have been successfully introduced into mesoporous silica nanoparticles (MSNs) to obtain a nanoplatform for photodynamic therapy. Three brominated squaraine dyes which exhibited good PS performance in solution but a different behaviour
http://pubs.rsc.org/en/content/articlelanding/2017/dt/c7dt03735j
Comamonas koreensis sp. nov., a non-motile species from wetland in Woopo, Korea. | Microbiology SocietyComamonas koreensis sp. nov., a non-motile species from wetland in Woopo, Korea. | Microbiology Society
A bacterial strain, designated YH12T, was isolated from a wetland sample collected from Woopo, Republic of Korea, and characterized using a polyphasic approach. Analysis of 16S rDNA indicated that the isolate formed a monophyletic clade with the members of the genus Comamonas. The closest phylogenetic relative among the valid species was Comamonas testosteroni, with 96.6% 16S rDNA similarity. The chemotaxonomic properties of the wetland isolate supported its membership of the genus Comamonas, as it contained ubiquinone Q-8 as a major respiratory quinone and hexadecanoic, methylene-hexadecanoic and octadecenoic acids as major cellular fatty acids. The G+C content of the DNA was 66 mol%. The isolate is a gram-negative, non-pigmented, rod-shaped, oxidase- and catalase-positive, non-motile, non-endospore-forming and non-fermentative bacterium. The phenotypic properties of the isolate were compared with those of the type strains of Comamonas terrigena, C. testosteroni and Delftia acidovorans. A number of
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/00207713-52-2-377
Alfa Aesar™ 2-Chloroethyl p-toluenesulfonate, 97% 500g Alfa Aesar™ 2-Chloroethyl p-toluenesulfonate, 97%Alfa Aesar™ 2-Chloroethyl p-toluenesulfonate, 97% 500g Alfa Aesar™ 2-Chloroethyl p-toluenesulfonate, 97%
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1H,1H,5H-Octafluoropentyl p-toluenesulfonate 2264-00-8 H-NMR | C-NMR Spectral Analysis NMR Spectrum1H,1H,5H-Octafluoropentyl p-toluenesulfonate 2264-00-8 H-NMR | C-NMR Spectral Analysis NMR Spectrum
1H,1H,5H-Octafluoropentyl p-toluenesulfonate 2264-00-8 NMR spectrum, 1H,1H,5H-Octafluoropentyl p-toluenesulfonate H-NMR spectral analysis, 1H,1H,5H-Octafluoropentyl p-toluenesulfonate C-NMR spectral analysis ect.
http://www.molbase.com/en/hnmr_2264-00-8-moldata-9331.html
3-Methylquinoline Degradation Pathway3-Methylquinoline Degradation Pathway
This pathway was contributed by Sandy Etienne, University of Minnesota.. 3-Methhylquinoline can be found in groundwater near landfills containing azaarene contaminated wastes, near creosote wood preservation facilities, in aquifers affected by fossil fuel processing activities and in shale oil. This compound is a known carcinogen causing various health effects and environmental damage, thus its environmental fate and biodegradation is important (Schach et al., 1993). Comamonas testosteroni 63, from activated sludge, is known to degrade 3-methylquinoline to 2,5,6-trihydroxy-3-methylpyridine. This compound is degraded further to unknown products (Schach et al., 1993). Quinoline, the unsubstituted derivative of 3-methylquinoline, is degraded by Rhodococcus strain B1 in a similar manner to form 5-hydroxy-6-(3-carboxyoxopropenyl)-1H-2-pyridone, which is further degraded to unknown metabolites (Schwarz et al., 1989). The following is a text-format 3-methylquinoline pathway map. An organism which can ...
http://eawag-bbd.ethz.ch/mqn/mqn_map.html
Evidence of distinct pathways for bacterial degradation of the steroid compound cholate suggests the potential for metabolic...Evidence of distinct pathways for bacterial degradation of the steroid compound cholate suggests the potential for metabolic...
The distribution and the metabolic pathways of bacteria degrading steroid compounds released by eukaryotic organisms were investigated using the bile salt cholate as model substrate. Cholate-degrading bacteria could be readily isolated from freshwater environments. All isolated strains transiently released steroid degradation intermediates into culture supernatants before their further degradation. Cholate degradation could be initiated via two different reaction sequences. Most strains degraded cholate via a reaction sequence known from the model organism Pseudomonas sp. strain Chol1 releasing intermediates with a 3-keto-Δ,sup,1,4,/sup,-diene structure of the steroid skeleton. The actinobacterium Dietzia sp. strain Chol2 degraded cholate via a different and yet unexplored reaction sequence releasing intermediates with a 3-keto-Δ,sup,4,6,/sup,-diene-7-deoxy structure of the steroid skeleton such as 3,12-dioxo-4,6-choldienoic acid (DOCDA). Using DOCDA as substrate, two Alphaproteobacteria, ...
https://kops.uni-konstanz.de/handle/123456789/28424
Czars - who are empowered without authority « Dr Kents BlogCzars - who are empowered without authority « Dr Kents Blog
Sits on the Board of Chrysler which is now Auto Union owned. How did this happen?. Central Region Czar: Dennis Ross Believes US policy has caused Mid East wars. Obama apologist to the world. Anti gun and pro abortion.. Domestic Violence Czar: Lynn Rosenthal Director of the National Network to End Domestic Violence. Vicious anti male feminist. Supported male castration.. Drug Czar: Gil Kerlikowske Devoted lobbyist for every restrictive gun law proposal, Former Chief of Police in Liberal Seattle. Believes no American should own a firearm. Supports legalization of drugs.. Economic Czar: Paul Volcker Head of Fed Reserve under Jimmy Carter when US economy nearly failed (thats absolutely dumb - I remember 17% interest on home loans during Carters reign). Obama appointed head of the Economic Recovery Advisory Board which engineered the Obama economic disaster to US economy. Member of anti business Progressive Policy organization.. Energy and Environment Czar: Carol Brower Political Radical -Former ...
https://drkentshow.com/wordpress/?tag=czars-who-are-empowered-without-authority
List of U.S. executive branch czars - WikipediaList of U.S. executive branch czars - Wikipedia
The numbers are based upon the sortable list below. Please see it for details and references. Note that what is measured is the popularity of the word czar, rather than an objective measure of authority.. Also note that under George W. Bush only 33 Czar titles had been currently found, thus only 33 Czars, although many of these titles were used by several distinct individuals. For example, there has been an Assistant Secretary of Labor for Mine Safety and Health since the passage of the Mine Safety and Health Act of 1977, but the phrase mine safety czar was only applied to the position since the controversial appointment of Richard Stickler to the post in 2006. Similarly, there has been a director of the Office of Information and Regulatory Affairs since the office was created by the Paperwork Reduction Act of 1980, but the term regulatory czar was not applied to the post until 2001.. ...
https://en.wikipedia.org/wiki/List_of_czars_of_the_Obama_administration
Healthy Pharmaceutical CAS 107868-30-4 Anti Estrogen Steroids Compounds Aromasin Exemestane
kualitas Anti Estrogen Steroid produsen & eksportir - Beli Healthy Pharmaceutical CAS 107868-30-4 Anti Estrogen Steroids Compounds Aromasin Exemestane dari Cina produsen.
http://indonesian.steroid-rawpowder.com/sale-9408914-healthy-pharmaceutical-cas-107868-30-4-anti-estrogen-steroids-compounds-aromasin-exemestane.html
Walhout Lab - Page 7Walhout Lab - Page 7
Dietary composition has major effects on physiology. Here, we show that developmental rate, reproduction, and lifespan are altered in C. elegans fed Comamonas DA1877 relative to those fed a standard E. coli OP50 diet. We identify a set of genes that change in expression in response to this diet and use the promoter of one of these (acdh-1) as a dietary sensor. Remarkably, the effects on transcription and development occur even when Comamonas DA1877 is diluted with another diet, suggesting that Comamonas DA1877 generates a signal that is sensed by the nematode. Surprisingly, the developmental effect is independent from TOR and insulin signaling. Rather, Comamonas DA1877 affects cyclic gene expression during molting, likely through the nuclear hormone receptor NHR-23. Altogether, our findings indicate that different bacteria elicit various responses via distinct mechanisms, which has implications for diseases such as obesity and the interactions between the human microbiome and intestinal ...
http://walhoutlab.umassmed.edu/?paged=7
NPHCDA Urged to Make Its Procurement Data Available for Public Scrutiny | THISDAYLIVENPHCDA Urged to Make Its Procurement Data Available for Public Scrutiny | THISDAYLIVE
Out of the 18 PHCs covered in our report were randomly selected based on procurement data available on Budeshi for Benue, Lagos, Oyo, Osun and Ogun States.. We observed that PHCs in the South function better. The visits showed a clear distinction between those in the South and those in the Northern part of the country. Although, two PHCs in Oyo and Osun states could not be located, the PHCs in the South seemed better integrated with the communities and were more functional than those in the North built within the same time frame.. For instance, out of the six PHC constructed in Kano State in 2014, only three are active, one; built but not functioning. One could not be located and the other remaining one building under construction abandoned. In Benue State, one of the six PHCs is active. Four, built but not functioning and one, abandoned.. The best people to report on procurement performance of primary health care facilities are the beneficiaries of these services. However, the accuracy and ...
https://www.thisdaylive.com/index.php/2016/12/08/nphcda-urged-to-make-its-procurement-data-available-for-public-scrutiny/
RCSB PDB for 1OHSRCSB PDB for 1OHS
1OHS: Crystal Structure of 5-3-Ketosteroid Isomerase Mutant Y14F/D38N from Pseudomonas Testosteroni Complexed with Androstanedione
http://www.rcsb.org/pdb/explore/biologyAndChemistry.do?structureId=1OHS
Genetic polymorphism in the human UGT1A6 (planar phenol) UDP-glucuronosyltransferase: pharmacological implicationsGenetic polymorphism in the human UGT1A6 (planar phenol) UDP-glucuronosyltransferase: pharmacological implications
Two missense mutations were uncovered in the UGT1A6 (HLUG P1) cDNA which codes for a human phenol-metabolizing UDP-glucuronosyltransferase. The mutant and a wild-type UGT1A6 cDNAs were isolated from a custom synthesized human liver lambda Zap cDNA library. Both an A to G transition at nucleotide 541 …
https://pubmed.ncbi.nlm.nih.gov/9429234/?dopt=Abstract
Testosteroni mittaus ja korvaushoito - Ravinto - Suomi24 Keskustelut
Boys with low T may grow slowly, with no body hair and poorly developed muscles. One possible cause of too much testosterone in women Pewdiepie Tulot. So your total testosterone levels abnormal testosterone levels.. However, it is important to note that normal T depends the arm using a small needle. Testosterone is a hormone produced. How can Betamet treat my for muscle mass, fat loss.. The Pewdiepie Tulot testosterone is important a sample of blood from on a variety of factors. The blood test involves taking huoltoasemalla - tilanne tallentui videolle: hn tulee voittamaan tulevaisuudessa viel.. Viimeksi mainittu tuottovaade tyttyy jo ja Carlos energiayrittjyyteen liittyv uutisvirtaa niiden luotettavuutta.. Testosterone is the main sex is known as free testosterone. In infant boys and girls, should be higher than your may be more extreme.. This test measures your free enough to diagnose any underlying. A single test is not testosterone levels, instead of total.. Esimerkiksi Pijt-Hmeen ...
https://topplista24.com/ampparit-uutiset/testosteroni-testi.php
Nanotechnology Now - Press Release: In-cell molecular sieve from protein crystalNanotechnology Now - Press Release: In-cell molecular sieve from protein crystal
Extreme stability of polyhedra under harsh conditions is provided by dense packing of polyhedrin monomers in crystals with solvent channels of very low porosity, which, however, limits the incorporation of foreign particles. Research group led by Satoshi Abe and Takafumi Ueno at Tokyo Institute of Technology hypothesized that if a porous framework inside PhCs is extended without compromising crystal stability, PhCs can be used for accumulation and storage of exogenous molecules in living cells. As in natural PhCs, polyhedrin monomers form a trimer, the scientists assumed that if amino acid residues at the contact interface of each trimer are deleted, the porosity of the resulting crystals would be increased. To achieve this goal, they genetically engineered polyhedrin monomers, which were then expressed and self-assembled in Spodoptera frugiperda IPLB-Sf21AE, the larva of an armyworm moth, infected with baculovirus. The mutant PhCs maintained crystal lattice of the wild-type PhC but had ...
http://www.nanotech-now.com/news.cgi?story_id=54327
Multiplan PHCS Family Physicians: Book Online By Insurance, Reviews & ZIPMultiplan PHCS Family Physicians: Book Online By Insurance, Reviews & ZIP
Family Physicians that take Multiplan PHCS, See Reviews and Book Online Instantly. Its free! All appointment times are guaranteed by our dentists and doctors.
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Esterification Catalysis by Pyridinium p-Toluenesulfonate Revisited-Modification with a Lipid Chain for Improved Activities and...Esterification Catalysis by Pyridinium p-Toluenesulfonate Revisited-Modification with a Lipid Chain for Improved Activities and...
A free platform for explaining your research in plain language, and managing how you communicate around it - so you can understand how best to increase its impact.
https://www.growkudos.com/publications/10.1080%25252F00397911.2012.749990/reader
Czar Salt Nicotine Tubes - Vapor King
Black SALT 720mg/ml - Sold as a pack of 5 Pink SALT 750mg/ml - Sold as a pack of 5 Grey SALT 1500mg/ml - Sold as a pack of 4 Czar Nicotine - Concentrated USP nicotine solution made to convert 0mg e-liquid. Just add Czar Nic t
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cannabisnews.com: Drug Czar Challengedcannabisnews.com: Drug Czar Challenged
cannabisnews.com: Drug Czar Challenged Drug Czar Challenged Posted by CN Staff on May 30, 2005 at 08:18:43 PT By Charles M. Darlington Source: Virginia Gazette Williamsburg, VA -- I am quite sure that Gazette reporter Rusty Carter was unaware that Dr. Andrea Barthwell ( Drug forum draws national interest, May 25 Gazette) has been hired by a British company called GW Pharmaceuticals that is attempting to market a liquid version of marijuana called Sativex. It is indistinguishable from the crude drug, having all its components but recombined in liquid form.Dr. Barthwell earned a considerable amount of taxpayer money as deputy director of the Organization for National Drug Control Policy, railing against exactly the same drug. Now, instead of claiming that marijuana has absolutely no medicinal properties, she now states that only Sativex has medicinal properties, and the crude drug does not.The self-serving hypocrisy of this statement becomes glaringly obvious, and I am not surprised that she did ...
http://cannabisnews.com/news/thread20740.shtml
Russian says remains of last Tsars son identified | ReutersRussian says remains of last Tsars son identified | Reuters
Russia said on Wednesday that charred remains found in a pit belonged to Tsar Nicholas IIs only son and his daughter, exactly 90 years after the Bolsheviks shocked the world by murdering the last Tsar.
https://www.reuters.com/article/us-russia-tsar-idUSL1519544820080716?sp=1
News & Perspective | CIDRAPNews & Perspective | CIDRAP
CIDRAP News) - Test results on a cluster of Alabama patients hospitalized with similar respiratory symptoms indicated no unusual pathogens but instead a variety of common respiratory viruses and bacteria, state health officials announced today. ...
http://www.cidrap.umn.edu/news-perspective?f%5B0%5D=field_related_topics%3A49&f%5B1%5D=field_related_topics%3A80
Bioaugmentation and coexistence of two functionally similar bacterial strains in aerobic granules | Springer for Research &...Bioaugmentation and coexistence of two functionally similar bacterial strains in aerobic granules | Springer for Research &...
The survival of the inoculated microbial culture is critical for successful bioaugmentation but impossible to predict precisely. As an alternative strategy, bioaugmentation of a group of...
https://rd.springer.com/article/10.1007%2Fs00253-007-0917-9
Russians open coffin of next-to-last czar to collect DNA - AP News - Breaking NewsRussians open coffin of next-to-last czar to collect DNA - AP News - Breaking News
Russians open coffin of next-to-last czar to collect DNA - AP News: ST. PETERSBURG, Russia (AP) - Investigators have opened the coffin .12/11/2017 18:23:07PM EST.
https://townhall.com/news/world/2015/11/27/russians-open-coffin-of-nexttolast-czar-to-collect-dna-n2085892
STOP MARXISM: Obamas MARXIST CZARS!STOP MARXISM: Obamas MARXIST CZARS!
There are very few of us who know just what all of Obamas Czars do, as they quietly go about their work in the nations capital. This listing of their names and job descriptions should be educational to all Americans, no matter what your political leaning. If you resent the existence of these extra-governmental officials, with their very generous salaries funded by, you guessed it, you the taxpayer, then get angry with the one who put these characters on the payroll ...
http://notyrants.blogspot.com/2012/01/obamas-marxist-czars.html
Meet Bishop Garrison: Pentagons extremism czar - WND News CenterMeet Bishop Garrison: Pentagons extremism czar - WND News Center
You may not have heard of this man.. You may wish you still havent.. Bishop Garrison is the Pentagons new extremism czar, installing Marxist radical race theory, purging Donald Trump sympathies from military ranks, especially all officers, and a man with unprecedented new powers.. He is leading CEWG, the Countering Extremism Working Group, with orders to change the Pentagons definition of extremism, to stop Pentagon personnel from being recruited by extremist groups and to screen more aggressively to find hidden extremists.. If you are in any branch of the military, you can expect Garrison Gangsters to view your internet history for any traces of evidence that might trigger them.. What made him a favorite to serve in this role?. Last January, during the heat of the second impeachment trial, Garrison tweeted that civility toward President Trump or his supporters would be the death of America.. For Garrison, there is no both sides in this war. There is no room for nuance, he says. ...
https://www.wndnewscenter.org/meet-bishop-garrison-pentagons-extremism-czar/
How Ebola Science Should Guide Americas New Ebola CzarHow Ebola Science Should Guide Americas New Ebola Czar
Today, Ron Klain officially starts his job as the countrys first Ebola Czar. He may not be a scientist himself, but science should guide his policies.
https://www.vice.com/en/article/nemmaq/how-the-science-of-ebola-should-guide-americas-new-ebola-czar
Drug Czar Nominee: Renewed Focus on Prevention - ABC NewsDrug Czar Nominee: Renewed Focus on Prevention - ABC News
Gil Kerlikowske, the Seattle police chief who has been nominated to be the nations drug czar, said he would take a balanced approach to drug enforcement. He is expected to promote a renewed emphasis on drug treatment and prevention.
https://abcnews.go.com/Health/story?id=7233062&page=1
Six Drug Czars, and Between Them They Cant Muster a Decent Argument for Marijuana Prohibition - Reason.comSix Drug Czars, and Between Them They Cant Muster a Decent Argument for Marijuana Prohibition - Reason.com
Our opposition to legalizing marijuana is grounded not in ideology but in facts and experience, say drug czar Gil Kerlikowske
https://reason.com/2010/08/25/six-drug-czars-and-between-the/
Taivuta arvioida - taivutus suomeksi - chocolateunltd.store verbintaivuttaja
Finnish Sen vuoksi on helpompaa arvioida tarkkaan, mitk ovat hydyllisimmt. Although the occasional static shock applying any force to their Panacod 1g of touch of a energy is relatively low, and wont harm a healthy person.. Kempele Ojan Rauta judge from the sky it difficult to gauge whether.. Finnish Meidn pit tss tilanteessa assess the conflicts of interest. Finnish Viranomaisen tehtvn on kartoittaa itself and the fingertip is.. Companies and consumers are finding that it might rain later. Low potentials may not have any notable effect, but some their prospects are good or.. A capacitive touch sensor responds can be startling and even unpleasant, the amount of stored part of a human body, usually a fingertip. Capacitive touch screens dont require than typical electronic components, they are also mostly Vapaa Testosteroni by to use and design in objects.. Unless we are required to aktiivisesti S-ryhmn ja yhteistykumppaneiden palveluja sek Pänttää (2008) ett Pänttää kunhan globaalit pstt ...
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Epub Strategic Decision Making In Modern Manufacturing 2004Epub Strategic Decision Making In Modern Manufacturing 2004
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http://beyond-the-void.com/swf/library.php?q=epub-strategic-decision-making-in-modern-manufacturing-2004/
CARBOXYLIC ACIDS AND THEIR DERIVATIVES - PDFCARBOXYLIC ACIDS AND THEIR DERIVATIVES - PDF
ARBXYLI AIDS AND TEIR DERIVATIVES A STUDENT SULD BE ABLE T: 1. Give the IUPA name given the structure, and draw the structure given the name, of carboxylic acids and their metal salts, acyl chlorides,
http://docplayer.net/31064284-Carboxylic-acids-and-their-derivatives.html
PHCs - IDEAS FOR GOA
Today, most of the individual medical records are in their personal medical paper folder or on with general physician doctors machine in Goa or most of India. How can we take control of our own medica... Read more... ...
https://ideasforgoa.com/ip-tag/phcs/
Phenomenex HPLC Part: Shodex GPC® KF-805 High Performance, LC Column 300 x 8 mm, Ea (Part#: KF-805)Phenomenex HPLC Part: Shodex GPC® KF-805 High Performance, LC Column 300 x 8 mm, Ea (Part#: KF-805)
HPLC Part: KF-805 Shodex GPC® KF-805 High Performance, LC Column 300 x 8 mm, Ea Recomended Use: Separation of polymers Separation Mode: Gel Permeation Chromatography (GPC) Solid Support: Polymer Format: Column USP Designation: N/A
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Russian pancake czar opens joint in Manhattan - RT Business NewsRussian pancake czar opens joint in Manhattan - RT Business News
The Russian Teremok pancake chain has opened its first location in the US. Teremok is the countrys biggest maker of blini - thin, fried pancakes served with cheese, ham, caviar or other fillings.
https://www.rt.com/business/346639-teremok-russia-pancakes-us/
Poljot International Tsars of RussiaPoljot International Tsars of Russia
Movement: Automatic cal 2427 Slava with date & weekday, double mainspring barrel, 18 000 A/h, 21 rubies, shock protection, 42 h power reserve Case: Stainles
https://www.classic-watch.de/Poljot-International-Tsars-of-Russia_3_1
Sonys tech czar ankles
The article reports that Sony Corp. of America chief technology officer, Phil Wiser, has decided to take on the job of chairman at the digital home video startup company, Building B. The startup will focus on delivering digital entertainment into homes. While the company is still in its early stages, it is expected to provide software and hardware as well as distribute content itself ...
http://connection.ebscohost.com/c/articles/21065530/sonys-tech-czar-ankles
1.4bn pupil catch-up cash branded pitiful as recovery tsar admits more needed | Express & Star1.4bn pupil catch-up cash branded pitiful as recovery tsar admits more needed | Express & Star
The money will be used to offer students up to 100 million hours of tuition following school closures during the coronavirus lockdowns.
https://www.expressandstar.com/news/uk-news/2021/06/02/14bn-pupil-catch-up-cash-branded-pitiful-as-recovery-tsar-admits-more-needed/
At least 19 died in flawed GM cars, compensation czar rulesAt least 19 died in flawed GM cars, compensation czar rules
Ken Feinberg, the attorney overseeing a compensation fund for victims of GM cars, has so far linked 19 deaths to a serious flaw with the automakers ignition switches.
https://money.cnn.com/2014/09/15/news/companies/gm-compensation-feinberg/index.html
What NDDCs abandoned $70 million can do for Niger DeltaWhat NDDCs abandoned $70 million can do for Niger Delta
The $70 billion NDDC funds abandoned in a bank can construct 370 classrooms or build 555 PHCs in 185 LGAs, or purchase 9 radiotherapy machines
https://www.premiumtimesng.com/news/headlines/371222-what-nddcs-abandoned-70-million-can-do-for-niger-delta.html
Spac pytle a karimatkmy
Pot eby pro p enocov n . Spac pytle v mnoha maskovac ch vzorech. Velk v b r spyc ch pytl . Nab z me tak origin ln arm dn spac ky a origin ln arm dn deky. irok v b r karimatek r zn vh velikost a tlou ek.
http://www.armysurplus.cz/index.php?main_page=index&cPath=51_57
TollefFogHeen - Ubuntu WikiTollefFogHeen - Ubuntu Wiki
Im the AMD64 czar working on fixing bugs specific to the AMD64 platform. I am employed by Canonical Limited to work on improving Ubuntu. The easiest way to get hold of me is usually IRC where I go by the nick Mithrandir, but jabber ([email protected]) or email ([email protected]) also works. In the spirit of package maintainenance in Ubuntu, I dont own any packages, but I do certainly care more for some than for others. Most of the ones I care about are the same as in Debian, such as pyblosxom, mailman, sash and mozilla-thunderbird. One of the things I try to get into Ubuntu is proper multiarch support, which will enable us to get rid of the evil hacks that openoffice.org-amd64 and ia32-libs* are. TollefFogHeenActivity ...
https://wiki.ubuntu.com/TollefFogHeen?highlight=%28%5CbCategoryHomepage%5Cb%29
Things to do to support yourself in Autumn (and pep up your Lungs and Large Intestine)
Audacious Health, Acupuncture Landsborough, Acupuncture Sunshine Coast, Your partners in healing ... naturally! Natural Therapies business servicing Landsborough, Beerwah, Glasshouse Mountains, Mount Mellum, Bald Knob, Maleny, Peachester, Glen View, Mooloolah, Diamond Valley, Caloundra and with clients from all areas of the Sunshine Coast and Brisbane. Scalp acupuncture experts. Auricular Therapy experts. Offering Bioptrom Light therapy. We also offer Dry Needling however, ask us about how Acupuncture is superior.
https://www.audacioushealth.com.au/blog/2016/03/07/Things-to-do-to-support-yourself-in-Autumn-and-pep-up-your-Lungs-and-Large-Intestine.aspx
Comamonas testosteroniComamonas testosteroni
... is a Gram-negative soil bacterium. Strain I2gfp has been used in bioaugmentation trials, in attempts to ... Comamonas testosteroni is also a component in a microbial concoction Mammoth-P, developed by Mammoth microbe, that claims to ... Type strain of Comamonas testosteroni at BacDive - the Bacterial Diversity Metadatabase v t e (Articles with short description ... "Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2gfp". Appl. ...
https://en.wikipedia.org/wiki/Comamonas_testosteroni
Prephenate dehydrogenasePrephenate dehydrogenase
Comamonas testosteroni, and nostoc sp. Plant organisms: phaseolus coccineus, phaseolus vulgaris, vicia faba, vigna radiata ...
https://en.wikipedia.org/wiki/Prephenate_dehydrogenase
Steroid Delta-isomeraseSteroid Delta-isomerase
Horinouchi M, Kurita T, Hayashi T, Kudo T (October 2010). "Steroid degradation genes in Comamonas testosteroni TA441: Isolation ... Horinouchi M, Hayashi T, Kudo T (March 2012). "Steroid degradation in Comamonas testosteroni". The Journal of Steroid ... KSI has been studied extensively from the bacteria Comamonas testosteroni (TI), formerly referred to as Pseudomonas ... "Insights into the catalytic mechanism and active-site environment of Comamonas testosteroni delta 5-3-ketosteroid isomerase as ...
https://en.wikipedia.org/wiki/Steroid_Delta-isomerase
Xenophilus azovoransXenophilus azovorans
Comamonas testosteroni is a rare human pathogen associated with acute appendicitis. It is known to have extremely low virulence ... Bayhan, Gulsum (2013). "Comamonas testosteroni: An Unusual Bacteria Associated with Acute Appendicitis". Balkan Medical Journal ... Those relatives are as follows: Hydrogena, Acidovorax, Comamonas, and Xylophilus. Xenophilus azovorans can be set apart from ...
https://en.wikipedia.org/wiki/Xenophilus_azovorans
5,6-dihydroxy-3-methyl-2-oxo-1,2,5,6-tetrahydroquinoline dehydrogenase5,6-dihydroxy-3-methyl-2-oxo-1,2,5,6-tetrahydroquinoline dehydrogenase
XVII Degradation of 3-methylquinoline by Comamonas testosteroni 63". Biol. Chem. Hoppe-Seyler. 374 (3): 175-81. doi:10.1515/ ...
https://en.wikipedia.org/wiki/5,6-dihydroxy-3-methyl-2-oxo-1,2,5,6-tetrahydroquinoline_dehydrogenase
PolyhydroxybutyratePolyhydroxybutyrate
Comamonas testosteroni and Pseudomonas stutzeri were obtained from sea water. Few of these are capable of degrading at higher ... Pseudomonas lemoigne, Comamonas sp. Acidovorax faecalis, Aspergillus fumigatus and Variovorax paradoxus are soil microbes ...
https://en.wikipedia.org/wiki/Polyhydroxybutyrate
TSUP familyTSUP family
Locher, HH; Poolman, B; Cook, AM; Konings, WN (1993). "Uptake of 4-toluene sulfonate by Comamonas testosteroni T-2". Journal of ... TsaS of Comamonas testosteroni, has been reported to function in the uptake of 4-toluene sulfonate. None of these functional ... as part of a putatively two-component transport system for 4-toluenesulphonate in Comamonas testosteroni T-2". Biochemical ...
https://en.wikipedia.org/wiki/TSUP_family
Comamonas thiooxydansComamonas thiooxydans
nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti". Current Microbiology. 61 (4 ... nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti". Current Microbiology. 61 (4 ... Comamonas thiooxydans is a Gram-negative, rod-shaped bacterium from the genus Comamonas and family Comamonadaceae, which was ... Based on this genome, the GTDB assigns strain E6 to Comamonas thiooxydans. "Genus: Comamonas". Retrieved 2013-05-10. "Archived ...
https://en.wikipedia.org/wiki/Comamonas_thiooxydans
DHSADHSA
3,4-DHSA is also produced by other bacteria such as Comamonas testosteroni. A particular type of enzyme known as extradiol ... 17-dione in steroid degradation in Comamonas testosteroni TA441". J. Steroid Biochem. Mol. Biol. 92 (3): 143-54. doi:10.1016/j. ... "Steroid degradation gene cluster of Comamonas testosteroni consisting of 18 putative genes from meta-cleavage enzyme gene tesB ...
https://en.wikipedia.org/wiki/DHSA
Terephthalate 1,2-cis-dihydrodiol dehydrogenaseTerephthalate 1,2-cis-dihydrodiol dehydrogenase
Wang YZ, Zhou Y, Zylstra GJ (1995). "Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni ...
https://en.wikipedia.org/wiki/Terephthalate_1,2-cis-dihydrodiol_dehydrogenase
4-formylbenzenesulfonate dehydrogenase4-formylbenzenesulfonate dehydrogenase
"Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2". J. Bacteriol. 179 (3): 919- ... pathways for p-toluenecarboxylate and p-toluenesulfonate and their multicomponent oxygenases in Comamonas testosteroni strains ...
https://en.wikipedia.org/wiki/4-formylbenzenesulfonate_dehydrogenase
4-sulfobenzoate 3,4-dioxygenase4-sulfobenzoate 3,4-dioxygenase
Purification and properties of a desulphonative two-component enzyme system from Comamonas testosteroni T-2". Biochem. J. 274: ...
https://en.wikipedia.org/wiki/4-sulfobenzoate_3,4-dioxygenase
2-hydroxyquinoline 5,6-dioxygenase2-hydroxyquinoline 5,6-dioxygenase
6-dioxygenase from Comamonas testosteroni 63. The first two enzymes in quinoline and 3-methylquinoline degradation". Eur. J. ...
https://en.wikipedia.org/wiki/2-hydroxyquinoline_5,6-dioxygenase
Quinoline 2-oxidoreductaseQuinoline 2-oxidoreductase
6-dioxygenase from Comamonas testosteroni 63. The first two enzymes in quinoline and 3-methylquinoline degradation". Eur. J. ...
https://en.wikipedia.org/wiki/Quinoline_2-oxidoreductase
Alcohol dehydrogenase (azurin)Alcohol dehydrogenase (azurin)
"Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: structural basis for substrate ... of the interaction between PQQ and heme c in the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni". ... "Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni". The Biochemical Journal. 234 (3): 611-5. doi: ...
https://en.wikipedia.org/wiki/Alcohol_dehydrogenase_(azurin)
Terephthalate 1,2-dioxygenaseTerephthalate 1,2-dioxygenase
Schlafli HR, Weiss MA, Leisinger T, Cook AM (1994). "Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: ...
https://en.wikipedia.org/wiki/Terephthalate_1,2-dioxygenase
3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate dehydrogenase
4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2". FEMS Microbiol. Lett. 130: 97-102. doi:10.1111/j.1574-6968.1995 ...
https://en.wikipedia.org/wiki/(3S,4R)-3,4-dihydroxycyclohexa-1,5-diene-1,4-dicarboxylate_dehydrogenase
4-(hydroxymethyl)benzenesulfonate dehydrogenase4-(hydroxymethyl)benzenesulfonate dehydrogenase
... pathways for p-toluenecarboxylate and p-toluenesulfonate and their multicomponent oxygenases in Comamonas testosteroni strains ...
https://en.wikipedia.org/wiki/4-(hydroxymethyl)benzenesulfonate_dehydrogenase
Cis-2,3-dihydrobiphenyl-2,3-diol dehydrogenaseCis-2,3-dihydrobiphenyl-2,3-diol dehydrogenase
3-dihydroxybiphenyl dehydrogenase from Comamonas testosteroni B-356 and sequence of the encoding gene (bphB)". Appl. Environ. ...
https://en.wikipedia.org/wiki/Cis-2,3-dihydrobiphenyl-2,3-diol_dehydrogenase
4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate hydrolase
4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441". Applied and ...
https://en.wikipedia.org/wiki/4,5:9,10-diseco-3-hydroxy-5,9,17-trioxoandrosta-1(10),2-diene-4-oate_hydrolase
2-hydroxyhexa-2,4-dienoate hydratase2-hydroxyhexa-2,4-dienoate hydratase
4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441". Applied and ...
https://en.wikipedia.org/wiki/2-hydroxyhexa-2,4-dienoate_hydratase
ComamonasComamonas
... species are aerobic organisms and motile using bipolar or polar tufts of one to five flagella.Comamonas testosteroni ... Comamonas J.P. Euzéby: List of Prokaryotic names with Standing in Nomenclature v t e (Articles with short description, Short ... Comamonas is a genus of bacteria in the phylum Pseudomonadota. Like all Pseudomonadota, they are Gram-negative bacteria. ... "The Emergence of the Genus Comamonas as Important Opportunistic Pathogens". Pathogens. 11 (9): 1032. doi:10.3390/ ...
https://en.wikipedia.org/wiki/Comamonas
List of MeSH codes (B03)List of MeSH codes (B03)
Comamonas MeSH B03.440.400.425.292.150.750 - Comamonas testosteroni MeSH B03.440.400.425.292.170 - Delftia MeSH B03.440.400.425 ... Comamonas MeSH B03.660.075.102.150.750 - Comamonas testosteroni MeSH B03.660.075.102.170 - Delftia MeSH B03.660.075.102.170.030 ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(B03)
Diploderma splendidumDiploderma splendidum
Comamonas testosterone, Enterococcus cecorum, Desulfovibrio desulfuricans, Bartonella rattaustraliani, Yersinia ...
https://en.wikipedia.org/wiki/Diploderma_splendidum
Poly(3-hydroxybutyrate) depolymerasePoly(3-hydroxybutyrate) depolymerase
Comamonas testosterone, Pseudomonas stutzeri, are found in seawater and lakewater. Among the most studied, Alcaligenes faecalis ... Species such as Pseudomonas lemoigne, Comamonas sp. Acidovorax faecalis, Aspergillus fumigatus and Variovorax paradoxus have ...
https://en.wikipedia.org/wiki/Poly(3-hydroxybutyrate)_depolymerase
SCOPe 2.08: Species: Comamonas testosteroni [TaxId: 285]SCOPe 2.08: Species: Comamonas testosteroni [TaxId: 285]
Timeline for Species Comamonas testosteroni [TaxId:285] from d.129.3.0 automated matches: *Species Comamonas testosteroni [ ... PDB entries in Species: Comamonas testosteroni [TaxId: 285]:. *Domain(s) for 3gzx: *. Domain d3gzxa2: 3gzx A:179-457 [232355]. ... Lineage for Species: Comamonas testosteroni [TaxId: 285]. *Root: SCOPe 2.08 *. Class d: Alpha and beta proteins (a+b) [53931] ( ... Species Comamonas testosteroni [TaxId:285] from d.129.3.0 automated matches appears in SCOPe 2.07. ...
http://scop.berkeley.edu/sunid=232354
Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas...Design and evaluation of 16S rRNA sequence based oligonucleotide probes for the detection and quantification of Comamonas...
... testosteroni subgroup. The FISH probe and one of the PCR primer pairs are suitable for quantification of C. testosteroni in ... testosteroni. Additionally, we have successfully used quantitative PCR to follow the C. testosteroni abundance during a ... This has been shown by analysis of samples from an enrichment of activated sludge on testosterone resulting in an increase in ... The oligonucleotides presented here provide a useful tool to study C. testosteroni population dynamics in mixed microbial ...
https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-6-54/figures/3
Species: Comamonas testosteroniSpecies: Comamonas testosteroni
and Comamonas testosteroni comb. nov., with an emended description of the genus Comamonas. Int. J. Syst. Bacteriol. 1987; 37:52 ... and Comamonas testosteroni comb. nov., with an emended description of the genus Comamonas. Int. J. Syst. Bacteriol. 1987; 37:52 ... Basonym: Pseudomonas testosteroni Marcus and Talalay 1956 (Approved Lists 1980) Etymology: N.L. gen. n. testosteroni, of ... lpsn.dsmz.de/species/comamonas-testosteroni. Copy to clipboard. Link copied to clipboard ...
https://lpsn.dsmz.de/species/comamonas-testosteroni
Degradace trichloroethenu bakterií Comamonas testosteroni za snížených teplotDegradace trichloroethenu bakterií Comamonas testosteroni za snížených teplot
... DSpace Repository. * DSpace Home ... Degradace trichloroethenu bakterií Comamonas testosteroni za snížených teplot. Show full item record ... Trichlorethylen je možné rozložit bakterií Comamonas testosteroni a to kometabolicky, kdy je v buňce indukován enzym, v tomto ... Tato diplomová práce studovala schopnost bakteriální degradace trichlorethylenu kulturou Comamonas testosteroni RF2 za ...
https://digilib.k.utb.cz/handle/10563/51241
Characterization of a 3 alpha-hydroxysteroid dehydrogenase/carbonyl reductase from the gram-negative bacterium Comamonas...
Proteins similar to the 3 alpha-HSD were detected and characterized from Comamonas testosteroni strain ATCC 17454 and from a ... bile acids and xenobiotics since Comamonas testosteroni and related species are found in the intestinal tract of vertebrates ... no similarities could be detected between HSD 28 and a recently determined 3 alpha-HSD sequence from the ATCC 11996 Comamonas ... was isolated from a testosterone-induced bacterial extract and characterized. The enzyme (HSD 28) has a monomeric molecular ...
https://www.kennedy.ox.ac.uk/publications/108848
Adjustable bidirectional extracellular electron transfer between Comamonas testosteroni biofilms and electrode via distinct...Adjustable bidirectional extracellular electron transfer between Comamonas testosteroni biofilms and electrode via distinct...
Adjustable bidirectional extracellular electron transfer between Comamonas testosteroni biofilms and electrode via distinct ... Adjustable bidirectional extracellular electron transfer between Comamonas testosteroni biofilms and electrode via distinct ... Adjustable bidirectional extracellular electron transfer between Comamonas testosteroni biofilms and electrode via distinct ...
https://scelse.sg/publications/adjustable-bidirectional-extracellular-electron-transfer-between-comamonas-testosteroni-biofilms-and-electrode-via-distinct-electron-mediators/
Table - Nosocomial Infections with IMP-19−Producing Pseudomonas aeruginosa Linked to Contaminated Sinks, France - Volume 23,...
Comamonas testosteroni. Room 46. Sink. ND. ND. P. mendocina. Room 40. Shower drain. ND. E, F. ...
https://wwwnc.cdc.gov/eid/article/23/2/16-0649-t1
Marie Stiborova - NeL.eduMarie Stiborova - NeL.edu
OBJECTIVE: Comamonas testosteroni Pb50 is a microorganism that possesses high tolerance for phenol and shows strong phenol ... Comamonas testosteroni:enzymology, Electrophoresis, Polyacrylamide Gel, Extracellular Space:enzymology, Hydrogen-Ion ... characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni.. ... characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni. ...
https://www.nel.edu/stiborova-1706/?vp-page=4
1.3.99.17: quinoline 2-oxidoreductase - BRENDA Enzyme Database1.3.99.17: quinoline 2-oxidoreductase - BRENDA Enzyme Database
Comamonas testosteroni, Pseudomonas putida, Rhodococcus sp., Pseudomonas putida 86, Comamonas testosteroni 63, Rhodococcus sp. ... Quinoline 2-oxidoreductase and 2-oxo-1,2-dihydroquinoline 5,6-dioxygenase from Comamonas testosteroni 63. The first two enzymes ...
https://brenda-enzymes.org/all_enzymes.php?ecno=1.3.99.17&table=Reference
Entire Collection
Comamonas testosteroni. Secretion. Gen.Diagn.Dept., PHL, Göteborg, Sweden. 1968-08-30. ... Comamonas acidovorans. Human wound. J.-E.Brorson, PHL, Göteborg, Sweden. 1968-08-30. ...
https://ccug.se/collections/search?t=&sort=cc&records=50&collection=entire
On the Number of Steroid‐Binding Sites of Δ<sup>5</sup>‐3‐Oxosteroid...
Comamonas testosteroni Medicine & Life Sciences 17% * Enzymes Medicine & Life Sciences 16% * Binding Site Chemical Compounds 16 ... The number of steroid‐binding sites in Δ5‐3‐oxosteroid isomerase of Pseudomonas testosteroni (EC 5.3.3.1) has been determined ... abstract = "The number of steroid‐binding sites in Δ5‐3‐oxosteroid isomerase of Pseudomonas testosteroni (EC 5.3.3.1) has been ... N2 - The number of steroid‐binding sites in Δ5‐3‐oxosteroid isomerase of Pseudomonas testosteroni (EC 5.3.3.1) has been ...
https://jhu.pure.elsevier.com/en/publications/on-the-number-of-steroidbinding-sites-of-%CE%B4sup5sup3oxosteroid-isom
Jose Pruneda-PazJose Pruneda-Paz
... a LuxR-type transcription factor required for testosterone degradation in Comamonas testosteroni. J. Bacteriol. 186(5):1430- ...
https://biology.ucsd.edu/research/faculty/jprunedapaz.html
The Turkish Journal of Pediatrics
in 1.7%, Comamonas testosteroni in 1.2% of patients produced. ESBL positivity is present in 51 (36.7%) of 139 E. coli strains ...
http://www.turkishjournalpediatrics.org/abstract.php?lang=en&id=1883
Table - Nosocomial Infections with IMP-19−Producing Pseudomonas aeruginosa Linked to Contaminated Sinks, France - Volume 23,...
Comamonas testosteroni. Room 46. Sink. ND. ND. P. mendocina. Room 40. Shower drain. ND. E, F. ...
https://wwwnc-origin.cdc.gov/eid/article/23/2/16-0649-t1
Gary Owens Home Page, University of South AustraliaGary Owens Home Page, University of South Australia
... modeling and molecular docking to analyze the laccase delignification process of rice straw by Comamonas testosteroni FJ17, ...
https://people.unisa.edu.au/gary.owens
DeCSDeCS
Comamonas testosteroni - Preferred Concept UI. M0328428. Scope note. A species of gram-negative, aerobic rods formerly called ... It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or ... It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or ... Pseudomonas testosteroni Scope note:. Especie de bacilos gramnegativos aerobios, previamente conocido como Pseudomonas ...
https://decs.bvsalud.org/en/ths/resource/?id=34091
Code System Concept
Comamonas testosteroni (organism). Code System Preferred Concept Name. Comamonas testosteroni (organism). Concept Status. ...
https://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=43721005
probeBase 2016probeBase 2016
Comamonas testosteroni. Nm143-446-1. Nitrosomonas sp. Nm143-related clones. Nm143-446-2. Nitrosomonas sp. Nm143-related clones ...
https://probebase.csb.univie.ac.at/pb_results/categories/25/0
RedOxiBase: Orthogroup detailRedOxiBase: Orthogroup detail
Comamonas testosteroni. Betaproteobacteria. 1 Show Hide. Show tree. 6129. CtesDCMD. Double CMD (peroxidase activity). View. ...
https://peroxibase.toulouse.inra.fr/orthogroups/view_orthogroup/DCMD002
Routine Intraoperative Bacterial Culture May Be Needed in Complicated AppendicitisRoutine Intraoperative Bacterial Culture May Be Needed in Complicated Appendicitis
Comamonas testosteroni. 1 (50). 0 (0). 0 (0). -. 0 (0). 0 (0). 0 (0). 0 (0). 0 (0). 0 (0). 0 (0). 1 (50). 0 (0). -. 2 (100). 0 ...
https://coloproctol.org/journal/view.php?number=1738&viewtype=pubreader
Molecular Characterization of camphor utilizing bacterial isolates from refinery sludge and detection of target loci-Cytochrome...Molecular Characterization of camphor utilizing bacterial isolates from refinery sludge and detection of target loci-Cytochrome...
Molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from Comamonas testosteroni GZ39. Appl Environ ...
https://springerplus.springeropen.com/articles/10.1186/2193-1801-2-170
Pre GI: CDS description
Comamonas testosteroni CNB-2, complete genome. alpha/beta hydrolase fold protein. NC_013446:2919005:2938491. NC_013446:2919005 ... Comamonas testosteroni CNB-2, complete genome. alpha/beta hydrolase fold protein. NC_013446:3209000:3210133. NC_013446:3209000 ... Comamonas testosteroni CNB-2, complete genome. alpha/beta hydrolase fold protein. NC_013739:2057781:2079379. NC_013739:2057781 ...
http://pregi.bi.up.ac.za/pre_gi_cds_desc.php?cds_desc=Alpha/beta%20hydrolase%20fold%20protein
Akvarijske bakterije Microbe-Lift Special Blend - ZGD AkvaristikaAkvarijske bakterije Microbe-Lift Special Blend - ZGD Akvaristika
Bakterije Comamonas testosteroni pospešujejo nevtralizacijo klora. Bakterije Bacillus pumilus z razgradnjo odmrlih organizmov ...
https://www.zgd.si/trgovina/akvaristika/nega-in-priprava-vode/priprava-vode/bakterije/microbe-lift-special-blend/
Other Characterized Proteins Similar to gabD
EC 1.1.1.257 from Comamonas testosteroni (Pseudomonas testosteroni). P94682 TsaD (EC 1.2.1.62) from Comamonas testosteroni. ...
https://papers.genomics.lbl.gov/cgi-bin/curatedClusters.cgi?set=carbon&path=putrescine&step=gabD&close=1
HOMD :: Ribosomal Protein TreeHOMD :: Ribosomal Protein Tree
BLAST Against "Comamonas testosteroni ATCC 11996" Genome Sequence. Copy and paste sequence(s) below, preferably in FASTA format ...
https://ehomd.org/genome/blast?gid=SEQF2057
Jasons Guide to No-Till Organic Living Soil (Indoor and Outdoor) - Indica InfoJason's Guide to No-Till Organic Living Soil (Indoor and Outdoor) - Indica Info
Pseudomonas putida and Comamonas testosteroni. These bacteria were chosen for their high phosphorous cycling ability in soil. ...
https://indicainfo.com/2020/03/23/jasons-guide-to-no-till-living-soil-indoor-and-outdoor/?like_comment=641&_wpnonce=9e72dc989a
HOMD Conserved Protein TreeHOMD Conserved Protein Tree
Comamonas testosteroni. KF-1 (. 6,026,527 bps in 1. contig) 👄. 0.020938822. HMT-858. SEQF2047. Comamonas testosteroni. CNB-2 ( ... Comamonas testosteroni. ATCC 11996 (. 5,410,155 bps in 63. contigs) 👄. 0.014132636. HMT-858. SEQF2058. Comamonas testosteroni. ...
https://homd.org/genome/conserved_protein_tree?otid=833
HOMD Ribosomal Protein TreeHOMD Ribosomal Protein Tree
Comamonas testosteroni. S44 (. 5,534,155 bps in 112. contigs) 👄. 0.000912272. HMT-858. SEQF2047. Comamonas testosteroni. CNB-2 ... Comamonas testosteroni. KF-1 (. 6,026,527 bps in 1. contig) 👄. 0.000531364. HMT-858. SEQF2057. Comamonas testosteroni. ATCC ...
https://www.homd.org/genome/ribosomal_protein_tree?otid=849
PseudomonasSpeciesATCCProteinGenomeSequencesSpecificAdditionTerephthalate degradationPurificationDegradation genesTalalayGenesTsaRCarbonylOperonCharacterizationSpeciesComponent
Pseudomonas6
  • Tamaoka J, Ha DM, Komagata K. Reclassification of Pseudomonas acidovorans den Dooren de Jong 1926 and Pseudomonas testosteroni Marcus and Talalay 1956 as Comamonas acidovorans comb. (dsmz.de)
  • Proteins similar to the 3 alpha-HSD were detected and characterized from Comamonas testosteroni strain ATCC 17454 and from a commercially available steroid-induced extract of a patent Pseudomonas strain. (ox.ac.uk)
  • Comamonas testosteroni, Pseudomonas putida, Rhodococcus sp. (brenda-enzymes.org)
  • The number of steroid‐binding sites in Δ 5 ‐3‐oxosteroid isomerase of Pseudomonas testosteroni (EC 5.3.3.1) has been determined from measurements of the red shift of the ultraviolet chromo‐phore of 19‐nortestosterone upon binding to the enzyme. (elsevier.com)
  • Especie de bacilos gramnegativos aerobios, previamente conocido como Pseudomonas testosteroni. (bvsalud.org)
  • A species of gram-negative, aerobic rods formerly called Pseudomonas testosteroni. (bvsalud.org)
Species2
  • The broad substrate specificities of the characterized 3 alpha-HSD enzymes lead to the conclusion that they might participate in the intestinal bioactivation or inactivation of hormones, bile acids and xenobiotics since Comamonas testosteroni and related species are found in the intestinal tract of vertebrates including man. (ox.ac.uk)
  • It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or maleate as carbon sources. (bvsalud.org)
ATCC2
  • A new form of the NAD(P)-dependent 3 alpha-hydroxysteroid dehydrogenases (3 alpha-HSDs), present in the gram-negative bacterium Comamonas testosteroni ATCC 11996, was isolated from a testosterone-induced bacterial extract and characterized. (ox.ac.uk)
  • However, no similarities could be detected between HSD 28 and a recently determined 3 alpha-HSD sequence from the ATCC 11996 Comamonas strain. (ox.ac.uk)
Protein3
  • 3,17β-Hydroxysteroid dehydrogenase (3,17β-HSD) from C. testosteroni is a Testosterone -inducible protein and a key enzyme in steroid degradation. (idexlab.com)
  • Identification and isolation of a regulator protein for 3,17β-HSD expressional regulation in Comamonas testosteroni. (idexlab.com)
  • A recent sequence database search identified a putative protein from the opportunistic pathogen Comamonas testosteroni that exhibits similarity with the Pasteurella multocida GAG synthase PmHS1, which is responsible for the synthesis of a heparosan polysaccharide capsule. (rpi.edu)
Genome1
  • The genome of C. testosteroni ATCC11996 was sequenced in our previous work. (idexlab.com)
Sequences2
  • Two potential promoters and two repeat sequences (RS, 16 bp), spaced to each other by 1661 bp, were also found upstream of the βhsd gene C. testosteroni. (idexlab.com)
  • Our data reveal that the βhsd gene undergoes complex regulation involving the two promoters, a loop structure via the two repeat sequences, and the steroid Testosterone . (idexlab.com)
Specific1
  • In summary, this new biosensor system might be used for the specific determination of Testosterone . (idexlab.com)
Addition2
  • In addition, a brp gene knock-out mutant of C. testosteroni was prepared. (idexlab.com)
  • Subsequently, biodegradation of PCP was studied in the same types of sterilized soils bioaugmented with bacterial isolate Comamonas testosteroni CCM 7350, with and without addition of OMC. (chemicke-listy.cz)
Terephthalate degradation2
  • Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni YZW-D. (nih.gov)
  • 2006). "Characterization of the terephthalate degradation genes of Comamonas sp. (igem.org)
Purification1
  • 4-Toluene sulfonate methyl-monooxygenase from Comamonas testosteroni T-2: purification and some properties of the oxygenase component. (genome.jp)
Degradation genes2
  • Steroid degradation genes in Comamonas testosteroni TA441: Isolation of genes encoding a Delta4(5)-isomerase and 3alpha- and 3beta-dehydrogenases and evidence for a 100 kb steroid degradation gene hot spot. (genome.jp)
  • 2010). "Characterization of the isophthalate degradation genes of Comamonas sp. (igem.org)
Talalay1
  • Comamonas testosteroni (Marcus and Talalay 1956) Tamaoka et al. (go.jp)
Genes1
  • a repressor of cbaABC, the 3-chlorobenzoic acid catabolic genes of comamonas testosteroni BR60 (pBRC60). (carleton.ca)
TsaR2
  • Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2. (genome.jp)
  • Junker, F., Kiewitz, R. & Cook, A. M. Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2. (energia2012.it)
Carbonyl2
  • Carbonyl reduction by 3 alpha-HSD from Comamonas testosteroni--new properties and its relationship to the SCAD family. (ox.ac.uk)
  • 2000. 'The crystal structure of 3alpha -hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni shows a novel oligomerization pattern within the short chain dehydrogenase/reductase family ', J Biol Chem , 275: 41333-9. (uni-wuerzburg.de)
Operon1
  • 2010). "Characterization of the protocatechuate 4,5-cleavage pathway operon in Comamonas sp. (igem.org)
Characterization2
  • Isolation and partial characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni. (nel.edu)
  • Turek M, Vilimkova L, Kremlackova V, Paca J, Halecky M, Paca J, Stiborova M. Isolation and partial characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni. (nel.edu)
Species1
  • It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or maleate as carbon sources. (nih.gov)
Component1
  • The enzyme 3beta/17beta-hydroxysteroid dehydrogenase (3beta/17beta-HSD) is a steroid-inducible component of the Gram-negative bacterium Comamonas testosteroni. (ox.ac.uk)