A species of gram-negative, aerobic rods formerly called Pseudomonas testosteroni. It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or maleate as carbon sources.
A genus of gram-negative, straight or slightly curved rods which are motile by polar flagella and which accumulate poly-beta-hydroxybutyrate within the cells.
A large group of aerobic bacteria which show up as pink (negative) when treated by the gram-staining method. This is because the cell walls of gram-negative bacteria are low in peptidoglycan and thus have low affinity for violet stain and high affinity for the pink dye safranine.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
A group of gram-negative bacteria consisting of rod- and coccus-shaped cells. They are both aerobic (able to grow under an air atmosphere) and microaerophilic (grow better in low concentrations of oxygen) under nitrogen-fixing conditions but, when supplied with a source of fixed nitrogen, they grow as aerobes.
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
A 3-hydroxysteroid dehydrogenase which catalyzes the reversible reduction of the active androgen, DIHYDROTESTOSTERONE to 5 ALPHA-ANDROSTANE-3 ALPHA,17 BETA-DIOL. It also has activity towards other 3-alpha-hydroxysteroids and on 9-, 11- and 15- hydroxyprostaglandins. The enzyme is B-specific in reference to the orientation of reduced NAD or NADPH.
A genus of gram-negative, aerobic, rod-shaped bacteria widely distributed in nature. Some species are pathogenic for humans, animals, and plants.
Non-heme iron-containing enzymes that incorporate two atoms of OXYGEN into the substrate. They are important in biosynthesis of FLAVONOIDS; GIBBERELLINS; and HYOSCYAMINE; and for degradation of AROMATIC HYDROCARBONS.
Enzymes of the oxidoreductase class that catalyze the dehydrogenation of hydroxysteroids. (From Enzyme Nomenclature, 1992) EC 1.1.-.
Catalyzes the oxidation of catechol to 2-hydroxymuconate semialdehyde in the carbazole and BENZOATE degradation via HYDROXYLATION pathways. It also catalyzes the conversion of 3-methylcatechol to cis, cis-2-hydroxy-6-oxohept-2,4-dienoate in the TOLUENE and XYLENE degradation pathway. This enzyme was formerly characterized as EC 1.13.1.2.
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
Steroids in which fission of one or more ring structures and concomitant addition of a hydrogen atom at each terminal group has occurred.
An antiseptic and disinfectant aromatic alcohol.
Benzoate derivatives substituted by one or more hydroxy groups in any position on the benzene ring.
Organic salts and esters of benzenesulfonic acid.
A pyrrolo-quinoline having two adjacent keto-groups at the 4 and 5 positions and three acidic carboxyl groups. It is a coenzyme of some DEHYDROGENASES.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Catalyze the oxidation of 3-hydroxysteroids to 3-ketosteroids.
A group of polycyclic compounds closely related biochemically to TERPENES. They include cholesterol, numerous hormones, precursors of certain vitamins, bile acids, alcohols (STEROLS), and certain natural drugs and poisons. Steroids have a common nucleus, a fused, reduced 17-carbon atom ring system, cyclopentanoperhydrophenanthrene. Most steroids also have two methyl groups and an aliphatic side-chain attached to the nucleus. (From Hawley's Condensed Chemical Dictionary, 11th ed)
A species of gram-negative rod-shaped bacteria found ubiquitously and formerly called Comamonas acidovorans and Pseudomonas acidovorans. It is the type species of the genus DELFTIA.
Enzymes that catalyze the transposition of double bond(s) in a steroid molecule. EC 5.3.3.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
A group of compounds that has the general structure of a dicarboxylic acid-substituted benzene ring. The ortho-isomer is used in dye manufacture. (Dorland, 28th ed)
An enzyme that catalyzes the conversion of 4,5-dihydro-4-oxo-5-imidazolepropanoate to urocanate and water. EC 4.2.1.49.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A potent androgenic steroid and major product secreted by the LEYDIG CELLS of the TESTIS. Its production is stimulated by LUTEINIZING HORMONE from the PITUITARY GLAND. In turn, testosterone exerts feedback control of the pituitary LH and FSH secretion. Depending on the tissues, testosterone can be further converted to DIHYDROTESTOSTERONE or ESTRADIOL.
The functional hereditary units of BACTERIA.
A metabolic process that converts GLUCOSE into two molecules of PYRUVIC ACID through a series of enzymatic reactions. Energy generated by this process is conserved in two molecules of ATP. Glycolysis is the universal catabolic pathway for glucose, free glucose, or glucose derived from complex CARBOHYDRATES, such as GLYCOGEN and STARCH.
Biosynthesis of GLUCOSE from nonhexose or non-carbohydrate precursors, such as LACTATE; PYRUVATE; ALANINE; and GLYCEROL.
The removal of a carboxyl group, usually in the form of carbon dioxide, from a chemical compound.
The bacterial sugar phosphotransferase system (PTS) that catalyzes the transfer of the phosphoryl group from phosphoenolpyruvate to its sugar substrates (the PTS sugars) concomitant with the translocation of these sugars across the bacterial membrane. The phosphorylation of a given sugar requires four proteins, two general proteins, Enzyme I and HPr and a pair of sugar-specific proteins designated as the Enzyme II complex. The PTS has also been implicated in the induction of synthesis of some catabolic enzyme systems required for the utilization of sugars that are not substrates of the PTS as well as the regulation of the activity of ADENYLYL CYCLASES. EC 2.7.1.-.
A group of enzymes that catalyzes the transfer of a phosphate group onto a nitrogenous group acceptor. EC 2.7.3.
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
An enzyme that catalyzes the oxidation of protocatechuate to 3-carboxy-cis-cis-muconate in the presence of molecular oxygen. It contains ferric ion. EC 1.13.11.3.
The CHEMICAL PROCESSES that occur within the cells, tissues, or an organism and related temporal, spatial, qualitative, and quantitative concepts.
DNA analogs containing neutral amide backbone linkages composed of aminoethyl glycine units instead of the usual phosphodiester linkage of deoxyribose groups. Peptide nucleic acids have high biological stability and higher affinity for complementary DNA or RNA sequences than analogous DNA oligomers.
Methods and techniques used to genetically modify cells' biosynthetic product output and develop conditions for growing the cells as BIOREACTORS.
The study, utilization, and manipulation of those microorganisms capable of economically producing desirable substances or changes in substances, and the control of undesirable microorganisms.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.
Industrial products consisting of a mixture of chlorinated biphenyl congeners and isomers. These compounds are highly lipophilic and tend to accumulate in fat stores of animals. Many of these compounds are considered toxic and potential environmental pollutants.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
The branch of medicine concerned with diseases, mainly of parasitic origin, common in tropical and subtropical regions.
Generally refers to the amount of protection available and the kind of loss which would be paid for under an insurance contract with an insurer. (Slee & Slee, Health Care Terms, 2d ed)
INFLAMMATION of the LIVER in humans caused by HEPATITIS C VIRUS, a single-stranded RNA virus. Its incubation period is 30-90 days. Hepatitis C is transmitted primarily by contaminated blood parenterally, and is often associated with transfusion and intravenous drug abuse. However, in a significant number of cases, the source of hepatitis C infection is unknown.
A branch of internal medicine concerned with the diagnosis and treatment of INFECTIOUS DISEASES.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
Aspects of health and disease related to travel.

Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441. (1/50)

Comamonas testosteroni TA441 degrades 3-(3-hydroxyphenyl)propionate (3HPP) via the meta pathway. A gene cluster required for degradation of 3HPP was cloned from strain TA441 and sequenced. The genes encoding six catabolic enzymes, a flavin-type hydroxylase (mhpA), extradiol dioxygenase (mhpB), 2-keto-4-pentenoate hydratase (mhpD), acetaldehyde dehydrogenase (acylating) (mhpF), 4-hydroxy-2-ketovalerate aldolase (mhpE) and the meta cleavage compound hydrolase (mhpC), were found in this cluster, encoded in this order. mhpD and mhpF were separated by two genes, orf4 and orf5, which were not necessary for growth on 3HPP. The gene mhpR, encoding a putative transcriptional activator of the IcIR family, was located adjacent to mhpA in the opposite orientation. Disruption of the mhpB or mhpR genes affected growth on 3HPP or trans-3-hydroxycinnamate. The mhpB and mhpC gene products showed high specificity for 3-(2,3-dihydroxyphenyl)propionate (DHPP) and the meta cleavage compound produced from DHPP, respectively.  (+info)

Crystal structure of delta(5)-3-ketosteroid isomerase from Pseudomonas testosteroni in complex with equilenin settles the correct hydrogen bonding scheme for transition state stabilization. (2/50)

Delta(5)-3-Ketosteroid isomerase from Pseudomonas testosteroni has been intensively studied as a prototype to understand an enzyme-catalyzed allylic isomerization. Asp(38) (pK(a) approximately 4.7) was identified as the general base abstracting the steroid C4beta proton (pK(a) approximately 12.7) to form a dienolate intermediate. A key and common enigmatic issue involved in the proton abstraction is the question of how the energy required for the unfavorable proton transfer can be provided at the active site of the enzyme and/or how the thermodynamic barrier can be drastically reduced. Answering this question has been hindered by the existence of two differently proposed enzyme reaction mechanisms. The 2.26 A crystal structure of the enzyme in complex with a reaction intermediate analogue equilenin reveals clearly that both the Tyr(14) OH and Asp(99) COOH provide direct hydrogen bonds to the oxyanion of equilenin. The result negates the catalytic dyad mechanism in which Asp(99) donates the hydrogen bond to Tyr(14), which in turn is hydrogen bonded to the steroid. A theoretical calculation also favors the doubly hydrogen-bonded system over the dyad system. Proton nuclear magnetic resonance analyses of several mutant enzymes indicate that the Tyr(14) OH forms a low barrier hydrogen bond with the dienolic oxyanion of the intermediate.  (+info)

Bioaugmentation of activated sludge by an indigenous 3-chloroaniline-degrading Comamonas testosteroni strain, I2gfp. (3/50)

A strain identified as Comamonas testosteroni I2 was isolated from activated sludge and found to be able to mineralize 3-chloroaniline (3-CA). During the mineralization, a yellow intermediate accumulated temporarily, due to the distal meta-cleavage of chlorocatechol. This strain was tested for its ability to clean wastewater containing 3-CA upon inoculation into activated sludge. To monitor its survival, the strain was chromosomally marked with the gfp gene and designated I2gfp. After inoculation into a lab-scale semicontinuous activated-sludge (SCAS) system, the inoculated strain maintained itself in the sludge for at least 45 days and was present in the sludge flocs. After an initial adaptation period of 6 days, complete degradation of 3-CA was obtained during 2 weeks, while no degradation at all occurred in the noninoculated control reactor. Upon further operation of the SCAS system, only 50% 3-CA removal was observed. Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA genes revealed a dynamic change in the microbial community structure of the activated sludge. The DGGE patterns of the noninoculated and the inoculated reactors evolved after 7 days to different clusters, which suggests an effect of strain inoculation on the microbial community structure. The results indicate that bioaugmentation, even with a strain originating from that ecosystem and able to effectively grow on a selective substrate, is not permanent and will probably require regular resupplementation.  (+info)

Arrangement and regulation of the genes for meta-pathway enzymes required for degradation of phenol in Comamonas testosteroni TA441. (4/50)

Comamonas testosteroni TA441 degrades phenol by a meta-cleavage pathway after the occurrence of a spontaneous mutation that derepresses the aphKLMNOPQB operon encoding phenol hydroxylase and catechol 2,3-dioxygenase, the enzymes for the initial two steps of the degradation pathway. A gene cluster, aphCEFGHJI, encoding the meta-pathway enzymes for degradation of 2-hydroxymuconic semialdehyde (HMS) to TCA cycle intermediates was found downstream of the aphK operon. The upstream operon and the downstream gene cluster were found to be separated by two open reading frames of unknown function and an oppositely oriented aphT gene, which is similar to regulatory genes for ortho-cleavage of catechol or chlorinated catechols. A promoter assay using an aphC::lacZ transcriptional fusion plasmid revealed that the aphC promoter activity is induced by both phenol and HMS. The phenol-dependent induction was mediated by AphR and the HMS-dependent induction was mediated by AphT. The aphC promoter in strain TA441 was not silenced, unlike the cases of the aphK and aphR promoters, and was highly induced by HMS.  (+info)

Regulation of the steroid-inducible 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase gene in Comamonas testosteroni. (5/50)

The Comamonas testosteroni 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase gene (hsdA) codes for an adaptive enzyme in the degradation of steroid compounds. However, no information was available on the molecular regulation of steroid-inducible genes nor on the mechanism of steroid signaling in procaryotes. We, therefore, investigated the cis- and trans-acting elements of hsdA expression to infer the mechanism of its molecular regulation by steroids. The gene was localized on a 5.257-kilobase EcoRI fragment of C. testosteroni chromosomal DNA. The promoter was characterized, and the transcriptional start site was identified. Two palindromic operator domains were found upstream of hsdA. A new gene coding for a trans-acting negative regulator (repressor A, RepA) of hsdA expression was characterized. The specific interaction between RepA, testosterone, and the operator domain is demonstrated. From our results we conclude that hsdA is under negative transcriptional control by an adjacent gene product (RepA). Accordingly, induction of hsdA by steroids in fact is a derepression, where steroidal inducers bind to the repressor, thereby preventing its binding to the hsdA operator.  (+info)

Map of the IncP1beta plasmid pTSA encoding the widespread genes (tsa) for p-toluenesulfonate degradation in Comamonas testosteroni T-2. (6/50)

The catabolic IncP1beta plasmid pTSA from Comamonas testosteroni T-2 was mapped by subtractive analysis of restriction digests, by sequencing outwards from the tsa operon (toluenesulfonate degradation), and by generating overlapping, long-distance-PCR amplification products. The plasmid was estimated to comprise 72 +/- 4 kb. The tsa region was found to be a composite transposon flanked by two IS1071 elements. A cryptic tsa operon was also present in the tsa transposon. Those backbone genes and regions which we sequenced were in the same order as the corresponding genes in resistance plasmid R751, and identities of about 99% were observed. Enrichment cultures with samples from four continents were done to obtain organisms able to utilize p-toluenesulfonate as the sole source of carbon and energy for aerobic growth. Most (15) of the 16 cultures (13 of them isolates) were obtained from contaminated sites and were attributed to three metabolic groups, depending on their metabolism of p-toluenesulfonate. The largest group contained the tsa transposon, usually (six of seven isolates) with negligible differences in sequence from strain T-2.  (+info)

Endotoxic properties of lipid A from Comamonas testosteroni. (7/50)

The lipid A from Comamonas testosteroni has been isolated and its complete chemical structure determined [Iida, T., Haishima, Y., Tanaka, A., Nishijima, K., Saito, S. & Tanamoto, K. (1996). Eur J Biochem 237, 468-475]. In this work, the relationship between its chemical structure and biological activity was studied. The lipid A was highly homogeneous chemically and was characterized by the relatively short chain length (C(10)) of the 3-hydroxy fatty acid components directly bound to the glucosamine disaccharide backbone by either amide or ester linkages. The lipid A exhibited endotoxic activity in all of the assay systems tested (mitogenicity in mouse spleen cells; induction of tumour necrosis factor alpha release from both mouse peritoneal macrophages and mouse macrophage-like cell line J774-1, as well as from the human monocytic cell line THP-1; induction of nitric oxide release from J774-1 cells; Limulus gelation activity and lethal toxicity in galactosamine-sensitized mice) to the same extent as did 'Salmonella minnesota' lipid A or Escherichia coli LPS used as controls. The strong endotoxic activity of the C. testosteroni lipid A indicates that the composition of 3-hydroxydecanoic acid is not responsible for the low endotoxicity of the lipid A observed in members of the genus Rhodopseudomonas, as has previously been suggested. Furthermore, both the lack of a second acylation of the 3-hydroxy fatty acid attached at the 3' position, and the substitution of the hydroxyl group of the 3-hydroxy fatty acid attached at position 2, do not affect the manifestation of endotoxic activity or species specificity.  (+info)

PhcS represses gratuitous expression of phenol-metabolizing enzymes in Comamonas testosteroni R5. (8/50)

We identified an open reading frame, designated phcS, downstream of the transcriptional activator gene (phcR) for the expression of multicomponent phenol hydroxylase (mPH) in Comamonas testosteroni R5. The deduced product of phcS was homologous to AphS of C. testosteroni TA441, which belongs to the GntR family of transcriptional regulators. The transformation of Pseudomonas aeruginosa PAO1c (phenol negative, catechol positive) with pROR502 containing phcR and the mPH genes conferred the ability to grow on phenol, while transformation with pROR504 containing phcS, phcR, and mPH genes did not confer this ability. The disruption of phcS in strain R5 had no effect on its phenol-oxygenating activity in a chemostat culture with phenol. The phenol-oxygenating activity was not expressed in strain R5 grown in a chemostat with acetate. In contrast, the phenol-oxygenating activity in the strain with a knockout phcS gene when grown in a chemostat with acetate as the limiting growth factor was 66% of that obtained in phenol-grown cells of the strain with a knockout in the phcS gene. The disruption of phcS and/or phcR and the complementation in trans of these defects confirm that PhcS is a trans-acting repressor and that the unfavorable expression of mPH in the phcS knockout cells grown on acetate requires PhcR. These results show that the PhcS protein repressed the gratuitous expression of phenol-metabolizing enzymes in the absence of the genuine substrate and that strain R5 acted by an unknown mechanism in which the PhcS-mediated repression was overcome in the presence of the pathway substrate.  (+info)

Comamonas testosteroni ATCC ® 700441D-5™ Designation: Genomic DNA from Comamonas testosteroni strain JS42 TypeStrain=False Application:
Comamonas testosteroni strain CNB-1 was isolated from activated sludge and has been investigated for its ability to degrade 4-chloronitrobenzene. Results from this study showed that strain CNB-1 grew on phenol, gentisate, vanillate, 3-hydroxybenzoate (3HB), and 4-hydroxybenzoate (4HB) as carbon and …
Selenium (Se) is an essential trace element in most organisms but has to be carefully handled since there is a thin line between beneficial and toxic concentrations. Many bacteria have the ability to reduce selenite (Se(IV)) and (or) selenate (Se(VI)) to red elemental selenium that is less toxic. A strictly aerobic bacterium, Comamonas testosteroni S44, previously isolated from metal(loid)-contaminated soil in southern China, reduced Se(IV) to red selenium nanoparticles (SeNPs) with sizes ranging from 100 to 200 nm. Both energy dispersive X-ray Spectroscopy (EDX or EDS) and EDS Elemental Mapping showed no element Se and SeNPs were produced inside cells whereas Se(IV) was reduced to red-colored selenium in the cytoplasmic fraction in presence of NADPH. Tungstate inhibited Se(VI) but not Se(IV) reduction, indicating the Se(IV)-reducing determinant does not contain molybdenum as co-factor. Strain S44 was resistant to multiple heavy and transition metal(loid)s such as Se(IV), As(III), Cu(II), and Cd(II)
Glycolysis is the process of converting glucose into pyruvate and generating small amounts of ATP (energy) and NADH (reducing power). It is a central pathway that produces important precursor metabolites: six-carbon compounds of glucose-6P and fructose-6P and three-carbon compounds of glycerone-P, glyceraldehyde-3P, glycerate-3P, phosphoenolpyruvate, and pyruvate [MD:M00001]. Acetyl-CoA, another important precursor metabolite, is produced by oxidative decarboxylation of pyruvate [MD:M00307]. When the enzyme genes of this pathway are examined in completely sequenced genomes, the reaction steps of three-carbon compounds from glycerone-P to pyruvate form a conserved core module [MD:M00002], which is found in almost all organisms and which sometimes contains operon structures in bacterial genomes. Gluconeogenesis is a synthesis pathway of glucose from noncarbohydrate precursors. It is essentially a reversal of glycolysis with minor variations of alternative paths [MD:M00003 ...
The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) is a major mechanism used by bacteria for uptake of carbohydrates, particularly hexoses, hexitols, and disaccharides, where the source of energy is from PEP. The PTS consists of two general components, enzyme I (EI) and histidine phosphocarrier protein (HPr), and of membrane-bound sugar specific permeases (enzymes II). Each enzyme II (EII) complex consists of one or two hydrophobic integral membrane domains (domains C and D) and two hydrophilic domains (domains A and B). EII complexes may exist as distinct proteins or as a single multidomain protein. The PTS catalyzes the uptake of carbohydrates and their conversion into their respective phosphoesters during transport. There are four successive phosphoryl transfers in the PTS. Initial autophosphorylation of EI, using PEP as a substrate, is followed by transfer of the phosphoryl group from EI to HPr. EIIA catalyzes the self-phosphoryl transfer from HPr after which the ...
CAS 68081-81-2 Sodium (C10-16)alkylbenzenesulfonate Chemical World Report This CAS 68081-81-2 - Sodium (C10-16)alkylbenzenesulfonate Chemical World...
Mutagenetic replacements of conserved residues within the active site of the short-chain dehydrogenase/reductase (SDR) superfamily were studied using prokaryotic 3 beta/17 beta-hydroxysteroid dehydrogenase (3 beta/17 beta-HSD) from Comamonas testosteroni as a model system. The results provide novel data to establish Ser 138 as a member of a catalytically important triad of residues also involving Tyr151 and Lys155. A Ser--|Ala exchange at position 138 results in an almost complete (| 99.9%) loss of enzymatic activity, which is not observed with a Ser--|Thr replacement. This indicates that an essential factor for catalysis is the ability of side chain 138 to form hydrogen bond interactions. Mutations in the NAD(H) binding region, in strands beta A, beta D, and adjacent turns, reveal two additional residues, Thr12 and Asn87, which are important for correct binding of the coenzyme and with a differential effect on the reactions catalyzed. Thus, mutation of Thr12 to Ala results in a complete loss of the 3
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Carbonyl reductase 3 catalyzes the reduction of a large number of biologically and pharmacologically active carbonyl compounds to their corresponding alcohols. The enzyme is classified as a monomeric NADPH-dependent oxidoreductase. CBR3 contains three exons spanning 11.2 kilobases and is closely linked to another carbonyl reductase gene - CBR1. [provided by RefSeq, Jul 2008 ...
The enzyme 3beta/17beta-hydroxysteroid dehydrogenase (3beta/17beta-HSD) is a steroid-inducible component of the Gram-negative bacterium Conramonas testosteroni. It catalyzes the reversible reduction/ dehydrogenation of the oxo/beta-hydroxy groups at positions 3 and 17 of steroid compounds, including hormones and isobile acids. Crystallographic analysis at 1.2 Angstrom resolution reveals the enzyme to have nearly identical subunits that form a tetramer with 222 symmetry. This is one of the largest oligomeric structures refined at this resolution. The subunit consists of a monomer with a single-domain structure built around a seven-stranded beta-sheet flanked by six alpha-helices. The active site contains a Ser-Tyr-Lys triad, typical for short-chain dehydrogenases/reductases (SDR). Despite their highly diverse substrate specificities, SDR members show a close to identical folding pattern architectures and a common catalytic mechanism. In contrast to other SDR apostructures determined, the ...
Elisa, dont come here and spout your opinion as fact. You will be denied because you are WRONG. You have been listening to Glenn Beck and Rush too long.. Check out this well researched wiki page on czars:. http://en.wikipedia.org/wiki/List_of_U.S._executive_branch_czars. It LISTS all the czars including ones appointed by Roosevelt. Hmmm… strange since you just claimed he never had any. Also Nixon, Lyndon Johnson, Clinton, Bush, and Obama. This page says Bush had 31 czars and 46 different people in the czar position. So perhaps my number of 36 IS off but it isnt even in the same ballpark as your figure… 12.. Your lowball figures are coming from conservative media who are nitpicking each and every czar under Bush and finding some teeny reason to say he was not REALLY a czar. They/you are seeing what you want to see.. But let us not forget that czars are essentially advisors. Czar is a media coined term. Can the President have too many advisors? Frankly I would HOPE a president would have a ...
White House Czar Calls for End to War on Drugs - WSJ.com WASHINGTON -- The Obama administrations new drug czar says he wants to banish the idea...
Trigger Warning: There are pictures in this article that may be upsetting to some. Viewer discretion is advised. Ello! This is the start of a new series that deal with mysterious diseases and unusual pathogens. The first of this showcase is the prion, a silent, mysterious and nigh-invulnerable killer with a 100% mortality rate. Lets start…
President Trumps coronavirus vaccine czar said Monday he expects Pfizers and Modernas Covid-19 shots will be effective against a new mutation of the...
Camera Lomo SP400CZ La Sardina Camera & Flash Czar on the price of 298lv. Specialized store with excellent prices and consultation. Free
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
TSAR is a free, digitally delivered art information utility. Every entry is carefully checked with the presenting institutions. Nevertheless, inaccuracies occur. Always try to call the sponsoring institution to check the facts. In our effort to be an increasingly more valuable product, we ask for your comments and suggestions to be emailed to [email protected] ...
Drug Czar Ramstad? Now that the State and Treasury department picks have been officially unveiled, the parlor game of Obama cabinet speculation turns to the less high-profile posts - the people...
August 26, 2004 - Washington, DC, USA Washington, DC: A newly released federal report refutes claims by US Drug Czar John Walters that the United States is being inundated with Canadian pot,...
The Washington Post and everybody else is reporting that on Tuesday President Obama will name Howard A. Schmidt as cyber-security czar. Schmidt was an advisor to President Bush on cyber-security matters. The Post rehearses the reasons why the Obama administration has had difficulty in finding someon...
Because Williams book follows closely historical events, anyone with a grasp of Russian history will know the outcome (though perhaps after Tarantinos Inglourious Basterds all bets are off when it comes to assassination attempts on real-life figures). It also means Hadfield is ultimately a rather ineffectual hero, flowing along with events with little power or opportunity to alter the fate of the Tsar or those who plan to kill him. ...
Washington is spending $60 billion to create the careers of the future, but not a single green job yet exists. Obamas green czar explains.
The Russian Orthodox Church posed a number of questions to the investigators and the state commission, which have remained unanswered
Why Is Wal-Mart Endorsing Employer Mandated Healthcare?... Tylenol Tsar FDA Panel Recommends Ban on Vicodin, Percocet... Candidates Aplenty for Spending on
I heard from General Carlito Galvez, chief implementor of COVID plans and vaccine czar, that the bulk of COVID-19 vaccines will arrive in the second quarter this year.
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Squaraine dyes, that exhibit intense absorption in the red/near infrared region, have been successfully introduced into mesoporous silica nanoparticles (MSNs) to obtain a nanoplatform for photodynamic therapy. Three brominated squaraine dyes which exhibited good PS performance in solution but a different behaviour
A bacterial strain, designated YH12T, was isolated from a wetland sample collected from Woopo, Republic of Korea, and characterized using a polyphasic approach. Analysis of 16S rDNA indicated that the isolate formed a monophyletic clade with the members of the genus Comamonas. The closest phylogenetic relative among the valid species was Comamonas testosteroni, with 96.6% 16S rDNA similarity. The chemotaxonomic properties of the wetland isolate supported its membership of the genus Comamonas, as it contained ubiquinone Q-8 as a major respiratory quinone and hexadecanoic, methylene-hexadecanoic and octadecenoic acids as major cellular fatty acids. The G+C content of the DNA was 66 mol%. The isolate is a gram-negative, non-pigmented, rod-shaped, oxidase- and catalase-positive, non-motile, non-endospore-forming and non-fermentative bacterium. The phenotypic properties of the isolate were compared with those of the type strains of Comamonas terrigena, C. testosteroni and Delftia acidovorans. A number of
1H,1H,5H-Octafluoropentyl p-toluenesulfonate 2264-00-8 NMR spectrum, 1H,1H,5H-Octafluoropentyl p-toluenesulfonate H-NMR spectral analysis, 1H,1H,5H-Octafluoropentyl p-toluenesulfonate C-NMR spectral analysis ect.
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This pathway was contributed by Sandy Etienne, University of Minnesota.. 3-Methhylquinoline can be found in groundwater near landfills containing azaarene contaminated wastes, near creosote wood preservation facilities, in aquifers affected by fossil fuel processing activities and in shale oil. This compound is a known carcinogen causing various health effects and environmental damage, thus its environmental fate and biodegradation is important (Schach et al., 1993). Comamonas testosteroni 63, from activated sludge, is known to degrade 3-methylquinoline to 2,5,6-trihydroxy-3-methylpyridine. This compound is degraded further to unknown products (Schach et al., 1993). Quinoline, the unsubstituted derivative of 3-methylquinoline, is degraded by Rhodococcus strain B1 in a similar manner to form 5-hydroxy-6-(3-carboxyoxopropenyl)-1H-2-pyridone, which is further degraded to unknown metabolites (Schwarz et al., 1989). The following is a text-format 3-methylquinoline pathway map. An organism which can ...
The distribution and the metabolic pathways of bacteria degrading steroid compounds released by eukaryotic organisms were investigated using the bile salt cholate as model substrate. Cholate-degrading bacteria could be readily isolated from freshwater environments. All isolated strains transiently released steroid degradation intermediates into culture supernatants before their further degradation. Cholate degradation could be initiated via two different reaction sequences. Most strains degraded cholate via a reaction sequence known from the model organism Pseudomonas sp. strain Chol1 releasing intermediates with a 3-keto-Δ,sup,1,4,/sup,-diene structure of the steroid skeleton. The actinobacterium Dietzia sp. strain Chol2 degraded cholate via a different and yet unexplored reaction sequence releasing intermediates with a 3-keto-Δ,sup,4,6,/sup,-diene-7-deoxy structure of the steroid skeleton such as 3,12-dioxo-4,6-choldienoic acid (DOCDA). Using DOCDA as substrate, two Alphaproteobacteria, ...
The numbers are based upon the sortable list below. Please see it for details and references. Note that what is measured is the popularity of the word czar, rather than an objective measure of authority.. Also note that under George W. Bush only 33 Czar titles had been currently found, thus only 33 Czars, although many of these titles were used by several distinct individuals. For example, there has been an Assistant Secretary of Labor for Mine Safety and Health since the passage of the Mine Safety and Health Act of 1977, but the phrase mine safety czar was only applied to the position since the controversial appointment of Richard Stickler to the post in 2006. Similarly, there has been a director of the Office of Information and Regulatory Affairs since the office was created by the Paperwork Reduction Act of 1980, but the term regulatory czar was not applied to the post until 2001.. ...
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Dietary composition has major effects on physiology. Here, we show that developmental rate, reproduction, and lifespan are altered in C. elegans fed Comamonas DA1877 relative to those fed a standard E. coli OP50 diet. We identify a set of genes that change in expression in response to this diet and use the promoter of one of these (acdh-1) as a dietary sensor. Remarkably, the effects on transcription and development occur even when Comamonas DA1877 is diluted with another diet, suggesting that Comamonas DA1877 generates a signal that is sensed by the nematode. Surprisingly, the developmental effect is independent from TOR and insulin signaling. Rather, Comamonas DA1877 affects cyclic gene expression during molting, likely through the nuclear hormone receptor NHR-23. Altogether, our findings indicate that different bacteria elicit various responses via distinct mechanisms, which has implications for diseases such as obesity and the interactions between the human microbiome and intestinal ...
1OHS: Crystal Structure of 5-3-Ketosteroid Isomerase Mutant Y14F/D38N from Pseudomonas Testosteroni Complexed with Androstanedione
Two missense mutations were uncovered in the UGT1A6 (HLUG P1) cDNA which codes for a human phenol-metabolizing UDP-glucuronosyltransferase. The mutant and a wild-type UGT1A6 cDNAs were isolated from a custom synthesized human liver lambda Zap cDNA library. Both an A to G transition at nucleotide 541 …
Boys with low T may grow slowly, with no body hair and poorly developed muscles. One possible cause of too much testosterone in women Pewdiepie Tulot. So your total testosterone levels abnormal testosterone levels.. However, it is important to note that normal T depends the arm using a small needle. Testosterone is a hormone produced. How can Betamet treat my for muscle mass, fat loss.. The Pewdiepie Tulot testosterone is important a sample of blood from on a variety of factors. The blood test involves taking huoltoasemalla - tilanne tallentui videolle: hn tulee voittamaan tulevaisuudessa viel.. Viimeksi mainittu tuottovaade tyttyy jo ja Carlos energiayrittjyyteen liittyv uutisvirtaa niiden luotettavuutta.. Testosterone is the main sex is known as free testosterone. In infant boys and girls, should be higher than your may be more extreme.. This test measures your free enough to diagnose any underlying. A single test is not testosterone levels, instead of total.. Esimerkiksi Pijt-Hmeen ...
Extreme stability of polyhedra under harsh conditions is provided by dense packing of polyhedrin monomers in crystals with solvent channels of very low porosity, which, however, limits the incorporation of foreign particles. Research group led by Satoshi Abe and Takafumi Ueno at Tokyo Institute of Technology hypothesized that if a porous framework inside PhCs is extended without compromising crystal stability, PhCs can be used for accumulation and storage of exogenous molecules in living cells. As in natural PhCs, polyhedrin monomers form a trimer, the scientists assumed that if amino acid residues at the contact interface of each trimer are deleted, the porosity of the resulting crystals would be increased. To achieve this goal, they genetically engineered polyhedrin monomers, which were then expressed and self-assembled in Spodoptera frugiperda IPLB-Sf21AE, the larva of an armyworm moth, infected with baculovirus. The mutant PhCs maintained crystal lattice of the wild-type PhC but had ...
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cannabisnews.com: Drug Czar Challenged Drug Czar Challenged Posted by CN Staff on May 30, 2005 at 08:18:43 PT By Charles M. Darlington Source: Virginia Gazette Williamsburg, VA -- I am quite sure that Gazette reporter Rusty Carter was unaware that Dr. Andrea Barthwell ( Drug forum draws national interest, May 25 Gazette) has been hired by a British company called GW Pharmaceuticals that is attempting to market a liquid version of marijuana called Sativex. It is indistinguishable from the crude drug, having all its components but recombined in liquid form.Dr. Barthwell earned a considerable amount of taxpayer money as deputy director of the Organization for National Drug Control Policy, railing against exactly the same drug. Now, instead of claiming that marijuana has absolutely no medicinal properties, she now states that only Sativex has medicinal properties, and the crude drug does not.The self-serving hypocrisy of this statement becomes glaringly obvious, and I am not surprised that she did ...
Russia said on Wednesday that charred remains found in a pit belonged to Tsar Nicholas IIs only son and his daughter, exactly 90 years after the Bolsheviks shocked the world by murdering the last Tsar.
CIDRAP News) - Test results on a cluster of Alabama patients hospitalized with similar respiratory symptoms indicated no unusual pathogens but instead a variety of common respiratory viruses and bacteria, state health officials announced today. ...
The survival of the inoculated microbial culture is critical for successful bioaugmentation but impossible to predict precisely. As an alternative strategy, bioaugmentation of a group of...
Russians open coffin of next-to-last czar to collect DNA - AP News: ST. PETERSBURG, Russia (AP) - Investigators have opened the coffin .12/11/2017 18:23:07PM EST.
There are very few of us who know just what all of Obamas Czars do, as they quietly go about their work in the nations capital. This listing of their names and job descriptions should be educational to all Americans, no matter what your political leaning. If you resent the existence of these extra-governmental officials, with their very generous salaries funded by, you guessed it, you the taxpayer, then get angry with the one who put these characters on the payroll ...
Today, Ron Klain officially starts his job as the countrys first Ebola Czar. He may not be a scientist himself, but science should guide his policies.
Gil Kerlikowske, the Seattle police chief who has been nominated to be the nations drug czar, said he would take a balanced approach to drug enforcement. He is expected to promote a renewed emphasis on drug treatment and prevention.
Our opposition to legalizing marijuana is grounded not in ideology but in facts and experience, say drug czar Gil Kerlikowske
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The Russian Teremok pancake chain has opened its first location in the US. Teremok is the countrys biggest maker of blini - thin, fried pancakes served with cheese, ham, caviar or other fillings.
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Comamonas testosteroni, and nostoc sp. Plant organisms: phaseolus coccineus, phaseolus vulgaris, vicia faba, vigna radiata ...
Comamonas testosteroni is a rare human pathogen associated with acute appendicitis. It is known to have extremely low virulence ... Bayhan, Gulsum (2013). "Comamonas testosteroni: An Unusual Bacteria Associated with Acute Appendicitis". Balkan Medical Journal ... Those relatives are as follows: Hydrogena, Acidovorax, Comamonas, and Xylophilus. Xenophilus azovorans can be set apart from ...
XVII Degradation of 3-methylquinoline by Comamonas testosteroni 63". Biol. Chem. Hoppe-Seyler. 374 (3): 175-81. doi:10.1515/ ...
Comamonas testosteroni and Pseudomonas stutzeri were obtained from sea water. Few of these are capable of degrading at higher ... Pseudomonas lemoigne, Comamonas sp. Acidovorax faecalis, Aspergillus fumigatus and Variovorax paradoxus are soil microbes ...
Locher, HH; Poolman, B; Cook, AM; Konings, WN (1993). "Uptake of 4-toluene sulfonate by Comamonas testosteroni T-2". Journal of ... TsaS of Comamonas testosteroni, has been reported to function in the uptake of 4-toluene sulfonate. None of these functional ... as part of a putatively two-component transport system for 4-toluenesulphonate in Comamonas testosteroni T-2". Biochemical ...
3,4-DHSA is also produced by other bacteria such as Comamonas testosteroni. A particular type of enzyme known as extradiol ... 17-dione in steroid degradation in Comamonas testosteroni TA441". J. Steroid Biochem. Mol. Biol. 92 (3): 143-54. doi:10.1016/j. ... "Steroid degradation gene cluster of Comamonas testosteroni consisting of 18 putative genes from meta-cleavage enzyme gene tesB ...
Wang YZ, Zhou Y, Zylstra GJ (1995). "Molecular analysis of isophthalate and terephthalate degradation by Comamonas testosteroni ...
"Characterization of the p-toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2". J. Bacteriol. 179 (3): 919- ... pathways for p-toluenecarboxylate and p-toluenesulfonate and their multicomponent oxygenases in Comamonas testosteroni strains ...
Purification and properties of a desulphonative two-component enzyme system from Comamonas testosteroni T-2". Biochem. J. 274: ...
6-dioxygenase from Comamonas testosteroni 63. The first two enzymes in quinoline and 3-methylquinoline degradation". Eur. J. ...
"Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: structural basis for substrate ... of the interaction between PQQ and heme c in the quinohemoprotein ethanol dehydrogenase from Comamonas testosteroni". ... "Quinohaemoprotein alcohol dehydrogenase apoenzyme from Pseudomonas testosteroni". The Biochemical Journal. 234 (3): 611-5. doi: ...
Schlafli HR, Weiss MA, Leisinger T, Cook AM (1994). "Terephthalate 1,2-dioxygenase system from Comamonas testosteroni T-2: ...
4-dicarboxylate dehydrogenase from Comamonas testosteroni T-2". FEMS Microbiol. Lett. 130: 97-102. doi:10.1111/j.1574-6968.1995 ...
... pathways for p-toluenecarboxylate and p-toluenesulfonate and their multicomponent oxygenases in Comamonas testosteroni strains ...
3-dihydroxybiphenyl dehydrogenase from Comamonas testosteroni B-356 and sequence of the encoding gene (bphB)". Appl. Environ. ...
4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441". Applied and ...
4-dienoic acid and related enzymes involved in testosterone degradation in Comamonas testosteroni TA441". Applied and ...
Comamonas MeSH B03.440.400.425.292.150.750 - Comamonas testosteroni MeSH B03.440.400.425.292.170 - Delftia MeSH B03.440.400.425 ... Comamonas MeSH B03.660.075.102.150.750 - Comamonas testosteroni MeSH B03.660.075.102.170 - Delftia MeSH B03.660.075.102.170.030 ...
Comamonas testosterone, Enterococcus cecorum, Desulfovibrio desulfuricans, Bartonella rattaustraliani, Yersinia ...
Comamonas testosterone, Pseudomonas stutzeri, are found in seawater and lakewater. Among the most studied, Alcaligenes faecalis ... Species such as Pseudomonas lemoigne, Comamonas sp. Acidovorax faecalis, Aspergillus fumigatus and Variovorax paradoxus have ...
nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti". Current Microbiology. 61 (4 ... nov., and comparison of thiosulfate oxidation with Comamonas testosteroni and Comamonas composti". Current Microbiology. 61 (4 ... Comamonas thiooxydans is a Gram-negative, rod-shaped bacterium from the genus Comamonas and family Comamonadaceae, which was ... Type strain of Comamonas thiooxydans at BacDive - the Bacterial Diversity Metadatabase v t e. ...
... is a Gram-negative soil bacterium. Strain I2gfp has been used in bioaugmentation trials, in attempts to ... Type strain of Comamonas testosteroni at BacDive - the Bacterial Diversity Metadatabase v t e. ... "Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2gfp". Appl. ... C testosteroni is also a component in a microbial concoction Mammoth-P, developed by Mammoth microbe, that claims to increase ...
Comamonas testosteroni and Pseudomonas stutzeri were obtained from sea water. Few of these are capable of degrading at higher ... Pseudomonas lemoigne, Comamonas sp. Acidovorax faecalis, Aspergillus fumigatus and Variovorax paradoxus are soil microbes ...
Comamonas testosteroni is a Gram-negative soil bacterium. Strain I2gfp has been used in bioaugmentation trials, in attempts to ... Type strain of Comamonas testosteroni at BacDive - the Bacterial Diversity Metadatabase v t e. ... "Bioaugmentation of Activated Sludge by an Indigenous 3-Chloroaniline-Degrading Comamonas testosteroni Strain, I2gfp". Appl. ... C testosteroni is also a component in a microbial concoction Mammoth-P, developed by Mammoth microbe, that claims to increase ...
Genomic DNA from Comamonas testosteroni strain JS42 TypeStrain=False Application: ... Comamonas testosteroni (Talalay) Tamaoka et al. emend. Willems et al. ATCC® 700441D-5™ freeze-dried Total DNA: At least 5 µg in ... Comamonas testosteroni (Talalay) Tamaoka et al. emend. Willems et al. (ATCC® 700441D-5™) Strain Designations: Genomic DNA from ... Genomic DNA from Comamonas testosteroni strain JS42 [ATCC® 700441™] Biosafety Level 1 Biosafety classification is based on U.S ...
Comamonas testosteroni (Pseudomonas testosteroni). ,p>This subsection of the ,a href="http://www.uniprot.org/help/names%5Fand% ... sp,Q59727,PHTD_COMTE 4,5-dihydroxyphthalate decarboxylase OS=Comamonas testosteroni OX=285 GN=phtD PE=4 SV=1 ...
Glycolysis / Gluconeogenesis - Comamonas testosteroni CNB-2 [ Pathway menu , Organism menu , Pathway entry , Download KGML , ...
Phosphotransferase system (PTS) - Comamonas testosteroni CNB-2 [ Pathway menu , Organism menu , Pathway entry , Download KGML ...
... hydroxysteroid dehydrogenase/carbonyl reductase from Comamonas testosteroni shows a novel oligomerization pattern within the ... Comamonas testosteroni [TaxId: 285] B. d1fjhb_. Alpha and beta proteins (a/b) NAD(P)-binding Rossmann-fold domains NAD(P)- ... THE CRYSTAL STRUCTURE OF 3-ALPHA-HYDROXYSTEROID DEHYDROGENASE FROM COMAMONAS TESTOSTERONI, A MEMBER OF THE SHORT CHAIN ...
Comamonas testosteroni strain CNB-1 was isolated from activated sludge and has been investigated for its ability to degrade 4- ... Comamonas testosteroni strain CNB-1 was isolated from activated sludge and has been investigated for its ability to degrade 4- ... Assimilation of aromatic compounds by Comamonas testosteroni: characterization and spreadability of protocatechuate 4,5- ... Results showed that the 4-oxalomesaconate (OMA) hydratase from C. testosteroni takes only enol-OMA as substrate. A previously ...
Three strains of Comamonas testosteroni were isolated from river sediment for the ability to degrade phenanthrene; two of the ... Molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from Comamonas testosteroni GZ39.. A K Goyal, ... Molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from Comamonas testosteroni GZ39. ... Molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from Comamonas testosteroni GZ39. ...
Comamonas testosteroni TA441 degrades steroids via aromatization of the A ring, followed by degradation of 9,17-dioxo-1,2,3,4, ... Steroid Degradation in Comamonas testosteroni TA441: Identification of the Entire β-Oxidation Cycle of the Cleaved B Ring. ... Steroid Degradation in Comamonas testosteroni TA441: Identification of the Entire β-Oxidation Cycle of the Cleaved B Ring ... This study aims to uncover the whole steroid degradation process in Comamonas testosteroni TA441 as a model of steroid- ...
In this study, using Comamonas testosteroni as a model organism, we demonstrated the involvement of Comamonas biofilms in ... Involvement in Denitrification is Beneficial to the Biofilm Lifestyle of Comamonas testosteroni: A Mechanistic Study and Its ... Comamonas is one of the most abundant microorganisms in biofilm communities driving wastewater treatment. Little has been known ... Our results showed that C. testosteroni could use nitrate as the sole electron acceptor for anaerobic growth. Under bulk ...
A new strain of Comamonas Testosteroni isolated from heavy-metal contaminated site in the Zambian Copperbelt: Adaption to ...
... testosteroni subgroup. The FISH probe and one of the PCR primer pairs are suitable for quantification of C. testosteroni in ... testosteroni. Additionally, we have successfully used quantitative PCR to follow the C. testosteroni abundance during a ... This has been shown by analysis of samples from an enrichment of activated sludge on testosterone resulting in an increase in ... The oligonucleotides presented here provide a useful tool to study C. testosteroni population dynamics in mixed microbial ...
Comamonas testosteroni T-2 was grown in salts medium containing intermediates of the established, inducible degradative pathway ... Comamonas testosteroni T-2 was grown in salts medium containing intermediates of the established, inducible degradative pathway ... Keyword(s): Cornamonas testosteroni , oxygenase systems , p-toluate , p-toluenesulphonate and regulation of aromatic catabolism ... of the degradative pathways from 4-toluenesulphonate and 4-toluenecarboxylate to protocatechuate in Comamonas testosteroni T-2 ...
Comamonas testosteroni (Talalay) Tamaoka et al. emend. Willems et al. (ATCC® 700441D-5™) ATCC® Number: 700441D-5™ Strain ...
Comamonas testosteroni. Loading... A0A076PQV4 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase. Comamonas testosteroni TK102 ...
Comamonas testosteroni Bacteremia: A Case Report and Review of the Literature. Abraham, JoEllyn M.; Simon, Gary L. ...
and Comamonas testosteroni comb. nov., with an emended description of the genus Comamonas ... Comamonas testosteroni strain ATCC 11996 LysR-type transcriptional regulator (hsdR) gene, complete cds. ATCC 11996 T ... Comamonas testosteroni strain ATCC 11996 short-chain alcohol dehydrogenases gene, complete cds. ATCC 11996 T ... Comamonas testosteroni ATCC 11996 contig81, whole genome shotgun sequence. ATCC 11996 T ...
Crystal Structure of 5-3-Ketosteroid Isomerase Mutant Y14F/D38N from Pseudomonas Testosteroni Complexed with Androstanedione ... Comamonas testosteroni EC#: 5.3.3.1 IUBMB Mutation: Y14F, D38N, T83I Gene Name(s): ksi ... CRYSTAL STRUCTURE OF 5-3-KETOSTEROID ISOMERASE MUTANT Y14F/D38N FROM PSEUDOMONAS TESTOSTERONI COMPLEXED WITH ANDROSTANEDIONE. * ...
Phenotypic methods; Comamonas testosteroni. D. tsuruhatensis (99%). (4). 2012, 53/F. Severe pulmonary hypertension. Yes. Blood ...
A locus harbouring genes from C. testosteroni BR6020 was cloned, dubbed pmd, which encodes the enzymes that degrade Pca. The ... This study provides evidence for a single lower pathway in C. testosteroni for metabolism of Pca, which is generated by ... A key intermediate for biodegradation of various distinct aromatic growth substrates in Comamonas testosteroni is ... 1997; Characterization of the p -toluenesulfonate operon tsaMBCD and tsaR in Comamonas testosteroni T-2. J Bacteriol179:919-927 ...
Comamonas testosteroni and Acinetobacter guillouiae Septic Shock due to Unusual Pathogens, Comamonas testosteroni and ... Acinetobacter , Bacteremia , Comamonas testosteroni , Comamonas , Communicable Diseases , Female , Humans , Korea , Renal ... Comamonas testosteroni and Acinetobacter guillouiae are gram-negative bacilli of low virulence that are widely distributed in ... Full text: Available Index: WPRIM (Western Pacific) Main subject: Shock, Septic / Testosterone / Virulence / Acinetobacter / ...
Comamonas testosteroni CNB-1. 852. Type IV secretion/conjugal transfer ATPase, VirB4 family protein. ACHXA ...
Comamonas testosteroni, and nostoc sp. Plant organisms: phaseolus coccineus, phaseolus vulgaris, vicia faba, vigna radiata ...
... while alcohol dehydrogenases found in Comamonas testosteroni, Cm. acidovorans and some Pseudomonas spp., have been shown to be ... Characterization of the gene encoding quinohaemoprotein ethanol dehydrogenase of Comamonas testosteroni. Eur. J. Biochem. 235, ... Quinohaemoprotein ethanol dehydrogenase from Comamonas testosteroni. Eur. J. Biochem. 230, 899-905. doi: 10.1111/j.1432- ... The Proteobacteria groups Pseudomonas, Thauera, Acinetobacter, Sphingobium, Decholoromonas, and Comamonas were amongst the ...
strain ADP1), AF150928.2; for CatR (P. putida), A35118; for AphT (Comamonas testosteroni TA441), BAA88500; for BenM ( ... strain U2), AF036940.2; for NbzR (Comamonas sp. strain JS765), AY223675.1; for NahR (Pseudomonas putida AN10), AF039534.1; for ...
Comamonas testosteroni. USteroid Delta-isomerase. Not Available. Pseudomonas putida. Absorption. Not Available. Volume of ...
Horinouchi M, Hayashi T, Kudo T (2012) Steroid degradation in Comamonas testosteroni. J Steroid Biochem Mol Biol 129:4-14. doi: ... Steroid degradation gene cluster of Comamonas testosteroni consisting of 18 putative genes from meta-cleavage enzyme gene tesB ... Part 2. Reduction of androstenedione to testosterone by Saccharomyces cerevisiae. Appl Microbiol Biotechnol 35:731-737. doi: ...
Goyal, A. K., and Zylstra, G. J. (1997). Genetics of naphthalene and phenanthrene degradation by Comamonas testosteroni. J. Ind ...
... and characterization of recombinant nitrile hydratase NI1 from Comamonas testosteroni. Protein Expr Purif 2003 May; 29(1): 70-6 ...
OBJECTIVE: Comamonas testosteroni Pb50 is a microorganism that possesses high tolerance for phenol and shows strong phenol ... Comamonas testosteroni:enzymology, Electrophoresis, Polyacrylamide Gel, Extracellular Space:enzymology, Hydrogen-Ion ... characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni.. ... characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni. ...
  • TAMAOKA (J.), HA (D.M.) and KOMAGATA (K.): Reclassification of Pseudomonas acidovorans den Dooren de Jong 1926 and Pseudomonas testosteroni Marcus and Talalay 1956 as Comamonas acidovorans comb. (bacterio.net)
  • Protein nomenclature is based on that for Rhodococcus jostii RHA1, Mycobacterium tuberculosis H37Rv, Comamonas testosteroni TA441, and Pseudomonas sp. (asm.org)
  • Reclassification of Pseudomonas acidovorans den Dooren de Jong 1926 and Pseudomonas testosteroni Marcus and Talahay 1956 as Comamonas acidovorans comb. (thefreedictionary.com)
  • A species of gram-negative, aerobic rods formerly called Pseudomonas testosteroni. (bvsalud.org)
  • Comamonas testosteroni strain CNB-1 was isolated from activated sludge and has been investigated for its ability to degrade 4-chloronitrobenzene. (nih.gov)
  • Delftia tsuruhatensis strain A90, Kocuria kristinae strain 6J-5b, Comamonas testosteroni WDL7, Stenotrophomonas maltophilia strain 776, Staphylococcus sp. (scielo.org.za)
  • Dijkstra, Bauke W. / Crystal structure of quinohemoprotein alcohol dehydrogenase from Comamonas testosteroni: Structural basis for substrate oxidation and electron transfer . (uea.ac.uk)
  • Molecular cloning of novel genes for polycyclic aromatic hydrocarbon degradation from Comamonas testosteroni GZ39. (asm.org)
  • The genes for naphthalene and phenanthrene degradation were cloned from one of the three C. testosteroni strains. (asm.org)
  • Southern blots also demonstrated that C. testosteroni GZ38A possesses genes for phenanthrene degradation that are similar to those cloned from C. testosteroni GZ39. (asm.org)
  • However, C. testosteroni GZ42 possesses genes for phenanthrene degradation that are not homologous to those cloned from C. testosteroni GZ39. (asm.org)
  • This suggests that there are at least two different sets of genes for the degradation of phenanthrene among the three C. testosteroni strains. (asm.org)
  • This study aims to uncover the whole steroid degradation process in Comamonas testosteroni TA441 as a model of steroid-degrading bacteria. (asm.org)
  • C. testosteroni is one of the most studied representative steroid-degrading bacteria and is suitable for exploring the degradation pathway, because the involvement of degradation-related genes can be determined by gene disruption. (asm.org)
  • Duc HD (2017) Degradation of chlorotoluenes by Comamonas testosterone KT5. (springer.com)
  • Characterized or annotated enzymes involved in the degradation of cholesterol by Actinobacteria are shown in red, those involved in the degradation of cholate by Actinobacteria are shown in lilac, and those involved in the degradation of testosterone or cholate by Proteobacteria are shown in blue. (asm.org)
  • Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441. (kegg.jp)
  • Comamonas testosteroni T-2 was grown in salts medium containing intermediates of the established, inducible degradative pathway(s) for 4-toluenesulphonate/4-toluenecarboxylate. (microbiologyresearch.org)
  • A key intermediate for biodegradation of various distinct aromatic growth substrates in Comamonas testosteroni is protocatechuate (Pca), which is metabolized by the 4,5-extradiol ( meta ) ring fission pathway. (microbiologyresearch.org)
  • This study provides evidence for a single lower pathway in C. testosteroni for metabolism of Pca, which is generated by different upper pathways acting on a variety of aromatic substrates. (microbiologyresearch.org)
  • This has been shown by analysis of samples from an enrichment of activated sludge on testosterone resulting in an increase in abundance and finally isolation of C. testosteroni . (biomedcentral.com)
  • Comamonas testosteroni 63, from activated sludge, is known to degrade 3-methylquinoline to 2,5,6-trihydroxy-3-methylpyridine. (ethz.ch)
  • O. anthropi es una bacteria emergente en infecciones intrahospitalarias con notable resistencia antimicrobiana, y es un patógeno inusual en humanos.A case report of bacteraemia by Ochrobactrum anthropi probably associated with bacterial hepatitis, in a inmuno competent patient, who was admitted to the hospital with the diagnostic impression of cholangitis and biliary obstruction. (worldwidescience.org)
  • In this study, using Comamonas testosteroni as a model organism, we demonstrated the involvement of Comamonas biofilms in denitrification under bulk aerobic conditions and elucidated the influence of nitrate respiration on its biofilm lifestyle. (ntu.edu.sg)
  • Fig. 1 shows a phylogenetic tree based on 16S rRNA sequences of species of the genera Comamonas and Delftia , with an emphasis on sequences of C. testosteroni isolates with more than 1200 bp length available from Ribosomal Database Project II. (biomedcentral.com)
  • Neighbour-joining tree of 16S rRNA gene sequences of species of the genera Comamonas and Delftia with an emphasis on Comamonas testosteroni corresponding to E. coli positions 135-1369. (biomedcentral.com)
  • Comamonas testosteroni (Talalay) Tamaoka et al. (atcc.org)
  • The β-proteobacterial species Comamonas testosteroni is capable of biotransformation and also biodegradation of a range of chemical compounds and thus potentially useful in chemical manufacturing and bioremediation. (biomedcentral.com)
  • Due to the widespread environmental distribution and the potential relevance of C. testosteroni in biotransformation processes, it may be of interest to follow the population dynamics of this species over time in a mixed-species environment. (biomedcentral.com)
  • Isolation and partial characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni. (nel.edu)
  • Turek M, Vilimkova L, Kremlackova V, Paca J, Halecky M, Paca J, Stiborova M. Isolation and partial characterization of extracellular NADPH-dependent phenol hydroxylase oxidizing phenol to catechol in Comamonas testosteroni. (nel.edu)
  • Comamonas testosteroni is a ubiquitously occuring β-proteobacterial species which has been isolated from aquatic as well as terrestrial environments. (biomedcentral.com)
  • It is differentiated from other Comamonas species by its ability to assimilate testosterone and to utilize phenylacetate or maleate as carbon sources. (bvsalud.org)
  • Two cosmid clones expressing polycyclic aromatic hydrocarbon dioxygenase activity were identified from a library prepared with genomic DNA from C. testosteroni GZ39. (asm.org)
  • Comamonas testosteroni BR6020 possesses a single genetic locus for extradiol cleavage of protocatechuateThe GenBank accession number for the sequence reported in this paper is AF305325. (microbiologyresearch.org)
  • Comamonas testosteroni Pb50 is a microorganism that possesses high tolerance for phenol and shows strong phenol degrading activ. (nel.edu)
  • A Southern blot with the cloned genes from C. testosteroni GZ39 confirmed that these genes are distinct from those found in P. putida NCIB 9816-4. (asm.org)
  • A locus harbouring genes from C. testosteroni BR6020 was cloned, dubbed pmd , which encodes the enzymes that degrade Pca. (microbiologyresearch.org)
  • Comamonas testosteroni is a Gram-negative soil bacterium. (wikipedia.org)
  • C testosteroni is also a component in a microbial concoction Mammoth-P, developed by Mammoth microbe, that claims to increase bioavailable phosphorus to growing plants and increase yield and stem strength in plants. (wikipedia.org)
  • The FISH probe and one of the PCR primer pairs are suitable for quantification of C. testosteroni in mixed microbial communities using FISH followed by quantitative image analysis or real-time quantitative PCR, respectively. (biomedcentral.com)
  • The oligonucleotides presented here provide a useful tool to study C. testosteroni population dynamics in mixed microbial communities. (biomedcentral.com)
  • nov., isolated from subterranean forest sediment, and emended description of the genus Comamonas . (bacterio.net)
  • Under bulk aerobic condition, biofilms of C. testosteroni were capable of reducing nitrate, and intriguingly, nitrate reduction significantly enhanced viability of the biofilm-cells and reduced cell detachment from the biofilms. (ntu.edu.sg)
  • Taking these factors together, this study reveals that nitrate reduction occurs in mature biofilms of C. testosteroni under bulk aerobic conditions, and the respiratory reduction of nitrate is beneficial to the biofilm lifestyle by providing more metabolic energy to maintain high viability and a higher level of c-di-GMP to reduce cell detachment. (ntu.edu.sg)
  • Inoculation with C. testosteroni reduced this negative impact on culturable bacterial numbers. (elsevier.com)
  • Results showed that the 4-oxalomesaconate (OMA) hydratase from C. testosteroni takes only enol-OMA as substrate. (nih.gov)
  • The following study reports on the development of 16S rRNA sequence based oligonucleotide probes which are suitable for detection and quantification of Comamonas testosteroni in mixed cultures using PCR and/or fluorescent in situ hybridization (FISH). (biomedcentral.com)
  • A short survey of 16S rRNA sequence diversity of C. testosteroni was carried out. (biomedcentral.com)
  • Holz, R. C., Analyzing the catalytic role of active site residues in the Fe-type nitrile hydratase from Comamonas testosteroni Ni1 . (luc.edu)
  • Our results showed that C. testosteroni could use nitrate as the sole electron acceptor for anaerobic growth. (ntu.edu.sg)
  • The ligand-binding domain of a chemoreceptor from Comamonas testosteroni has a previously unknown homotrimeric structure. (nih.gov)
  • C. testosteroni is thus of interest for potential biotechnological applications such as chemical transformations in fine chemical manufacturing [ 11 ] and bioremediation processes [ 7 , 12 ], as indicated by laboratory results. (biomedcentral.com)
  • Purification and properties of a desulphonative two-component enzyme system from Comamonas testosteroni T-2. (qmul.ac.uk)
  • Thus there is an methicillin-resistant drug to eventually be its sufficient testosteroni. (stovu.net)