Analytical, Diagnostic and Therapeutic Techniques and EquipmentHealth Care
Colony-Forming Units AssayIntensive Care Units
Colony-Forming Units Assay
A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
Intensive Care Units
Hospital units providing continuous surveillance and care to acutely ill patients.

Increase of hematopoietic responses by triple or single helical conformer of an antitumor (1-->3)-beta-D-glucan preparation, Sonifilan, in cyclophosphamide-induced leukopenic mice. (1/3030)

It has been suggested that the immunopharmacological activity of soluble (1-->3)-beta-D-glucan depends on its conformation in mice. In this study, we examined the relationship between the conformation of Sonifilan (SPG) and hematopietic responses in cyclophosphamide (Cy)-induced leukopenic mice. SPG, a high molecular weight (1-->3)-beta-D-glucan, has a triple helical conformation in water, and it was changed by treatment with aqueous sodium hydroxide to the single helical conformer (SPG-OH). The effects of SPG or SPG-OH on hematopoietic responses in cyclophosphamide induced leukopenic mice were investigated by monitoring i) gene expression of cytokines by RT-PCR, ii) protein synthesis of interleukin 6 (IL-6) by ELISA and iii) colony formation of bone marrow cells (BMC). The mice administered Cy and SPG or SPG-OH expressed and produced higher levels of IL-6 mRNA and protein than the mice administered only Cy. Gene expression of NK1.1 was also induced by Cy/SPG (or SPG-OH) treatment. Induced gene expression of stem cell factor (SCF) and macrophage-colony stimulating factor (M-CSF) by SPG/SPG-OH were also found in in vitro culture of BMC from Cy treated mice. These results strongly suggested that conformation of the glucans, single and triple helix, are independent of the hematopietic response.  (+info)

Functional characterization of a novel hematopoietic stem cell and its place in the c-Kit maturation pathway in bone marrow cell development. (2/3030)

While the majority of purified pluripotential hematopoietic stem cells (PHSC) express c-Kit, the receptor for steel factor, we have phenotypically and functionally separated a distinct class of PHSC that does not express c-Kit. In contrast to c-Kit-positive (c-Kit(pos)) PHSC, the c-Kit-negative (c-Kit(neg)) PHSC do not proliferate in response to multiple hematopoietic growth factors in vitro and do not radioprotect or form macroscopic spleen colonies (CFU-s) when transplanted into lethally irradiated recipients. However, the c-Kit(neg) PHSC show delayed or slow reconstitution kinetics when cotransplanted with radioprotective bone marrow cells. c-Kit(neg) PHSCs cells can give rise to c-Kit(pos) cells with CFU-s activity, radioprotective activity, and PHSC activity. Thus, constitutive hematopoiesis is maintained by c-Kit(pos) PHSCS cells that are recruited from a more primitive quiescent c-Kit(neg) PHSC population, which represents a critical developmental stage in definitive hematopoiesis.  (+info)

Single leukapheresis products collected from healthy donors after the administration of granulocyte colony-stimulating factor contain ten-fold higher numbers of long-term reconstituting hematopoietic progenitor cells than conventional bone marrow allografts. (3/3030)

Cytokine-mobilized peripheral blood progenitor cells (PBPCs) have been used successfully for hematopoietic reconstitution following allogeneic transplantation. The ease of harvest, the faster engraftment and the high yield of CD34+ cells have made this source of hematopoietic progenitor cells (HPCs) an attractive alternative to bone marrow (BM). In the present study we compared the engraftment potential of conventional BM allografts and single leukapheresis products (LPs) collected from healthy donors following the administration of granulocyte colony-stimulating factor (G-CSF). For this, lineage-committed and primitive HPCs were assessed by flow cytometry and by colony- and cobblestone area-forming cell (CFC, CAFC) assays. Mean numbers of CD34+ cells in LPs (n = 11) were similar to that of BM grafts (n = 12) (278+/-57 vs 227+/-34 x 10(6) CD34+ cells). The frequencies of CFCs, week 5 CAFCs and week 8 CAFCs were 1.6-, 8.4- and 10.3-fold higher in the CD34+ compartment of mobilized blood than that of marrow, resulting in significantly higher yields of clonogenic HPCs in LPs when compared to BM grafts. We conclude that G-CSF preferentially mobilizes clonogenic progenitors capable of short- and, in particular, longterm reconstitution, and that the engraftment potential of single LPs is superior to that of BM allografts. Hence, the use of PBPCs may be favorable for protocols that include graft manipulations with expected cell loss (eg T cell depletion, CD34+ selection). PBPCs may also be advantageous for gene therapy trials due to their high numbers of potential target cells (eg CAFCs).  (+info)

Influence of increased c-Myc expression on the growth characteristics of human melanoma. (4/3030)

Overexpression of the proto-oncogene c-myc has been associated with neoplastic transformation in a variety of tumors. For human melanoma high c-myc expression has been found in the vertical growth phase and higher positivity reported in metastases than primary tumors. The principle aim of this study was to determine, whether c-Myc expression influences the metastatic behavior of human melanoma in the absence of lymphocyte-mediated immune phenomena. The growth characteristics and tumor biology of two c-myc transfectants of the human melanoma cell line IGR39D, expressing c-Myc 1.7 and three times over baseline and the respective vector control were analyzed both in vitro and in a severe combined immunodeficient mouse model in vivo. Both c-myc transfectants showed increased growth rates, anchorage independent growth and directed cell movement in culture. Subcutaneously implanted IGR39D melanomas highly overexpressing c-Myc spontaneously formed macroscopic metastases (lymph nodes and lung) in severe combined immunodeficient mice in all cases (n = 7 per group), whereas less prominent c-Myc overexpression caused the development of only lung micrometastases. During the time period leading to terminal disease in animals injected with c-myc transfected human melanoma cells, melanoma development was not seen in vector controls. These findings suggest that constitutive high c-Myc expression in human melanoma results in a more aggressive growth behavior both in vitro and in vivo and favors metastasis in severe combined immunodeficient mice by factors unrelated to immune phenomena such as class I human leukocyte antigen downregulation known to be associated with c-Myc expression.  (+info)

Differential behaviors toward ultraviolet A and B radiation of fibroblasts and keratinocytes from normal and DNA-repair-deficient patients. (5/3030)

Xeroderma pigmentosum (XP) and trichothiodystrophy (TTD) are rare genodermatoses transmitted as recessive and autosomal traits that result in reduced capacity to repair UV-induced DNA lesions. Although XP, but not TTD, patients are prone to basal and squamous cell carcinomas, to date no comparative studies of the XP and TTD phenotypes have included epidermal keratinocytes. We compared the DNA repair capacity (by unscheduled DNA synthesis) and cell survival (by clonal analysis) of epidermal keratinocytes and dermal fibroblasts grown from normal individuals and patients with xeroderma pigmentosum and trichothiodystrophy following UVA and UVB irradiation. The same dose of UVB (1000 J/m2) induced twice as many DNA lesions in normal fibroblasts as in normal keratinocytes. UV survival rates were always higher in keratinocytes than in fibroblasts. Normal and TTD keratinocytes survived better following UVA and UVB irradiation than XP-C and XP-D keratinocytes. XP-C keratinocytes exhibited exacerbated sensitivity toward UVA radiation. Unscheduled DNA synthesis at UV doses leading to 50% cell survival indicated that the ratio of DNA repair capacity to cell survival is higher in keratinocytes than in fibroblasts. In addition, UVA and UVB irradiation induced a transition from proliferative to abortive keratinocyte colonies. This transition varied between donors and was in part correlated with their cancer susceptibility. Altogether these data provide the first evidence of the differential behaviors of normal, XP, and TTD keratinocytes toward UV radiation.  (+info)

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing. (6/3030)

Uncontrolled-rate freezing techniques represent an attractive alternative to controlled-rate cryopreservation procedures which are time-consuming and require high-level technical expertise. In this study, we report our experience using uncontrolled-rate cryopreservation and mechanical freezer storage at -140 degrees C. Twenty-eight PBPC samples (10 cryovials, 18 freezing bags) from 23 patients were cryopreserved in a cryoprotectant solution composed of phosphate-buffered saline (80%, v/v) supplemented with human serum albumin (10%, v/v) and dimethylsulfoxide (10%, v/v). The cryopreservation procedure required on average 1.5 h. The mean (+/- s.e.m.) storage time of cryovials and bags was 344+/-40 and 299+57 days, respectively. Although cell thawing was associated with a statistically significant reduction of the absolute number of nucleated cells (vials: 0.3x10(9) vs. 0.2x10(9), P< or =0.02; bags: 14x10(9) vs. 11x10(9), P< or =0.0003), the growth of committed progenitors was substantially unaffected by the freezing-thawing procedure, with mean recoveries of CFU-Mix, BFU-E, and CFU-GM ranging from 60+/- 29% to 134+/-15%. Mean recoveries of LTC-IC from cryovials and bags were 262+/-101% and 155+/-27% (P< or =0.2), respectively. In 14 out of 23 patients who underwent high-dose chemotherapy and PBPC reinfusion, the pre-and post-freezing absolute numbers of hematopoietic progenitors cryopreserved in bags were compared. A significant reduction was detected for CFU-Mix (11 vs. 7.4x10(5)), but no significant loss of BFU-E (180 vs. 150x10(5)), CFU-GM (400 vs. 290x10(5)) and LTC-IC (15 vs. 16x10(5)) could be demonstrated. When these patients were reinfused with uncontrolled-rate cryopreserved PBPC, the mean number of days to reach 1x10(9)/l white blood cells and 50x10(9)/l platelets were 9 and 13, respectively. In conclusion, the procedure described here is characterized by short execution time, allows a substantial recovery of primitive and committed progenitors and is associated with prompt hematopoietic recovery following myeloablative therapy even after long-term storage.  (+info)

Conclusions of a national multicenter intercomparative study of in vitro cultures of human hematopoietic progenitors. (7/3030)

With the aim of developing a standardized program of clonogenic cultures, a multicenter intercomparative study of human CFU-GM, BFU-E and CFU-GEMM cultures was conducted. Aliquots of fresh mononuclear cord blood cells, as well as uniform culture materials and instructions for cell culture and for colony scoring were distributed to 28 national laboratories involved in hematopoietic research and transplantation. High interlaboratory coefficients of variation (CV) in the reported number of progenitors were found in our first intercomparative study (range 67-231%). To investigate the relevance of colony scoring in variations of the reported colony numbers, participants were invited to attend a meeting where a single culture dish was scored. In this case, the CVs ranged from 31% to 81%. A subsequent intercomparative assay was then conducted, and significant reductions in the inter-laboratory CVs were obtained with respect to the first study (CVs for colonies grown with two different media: CFU-GMs, 48% and 55%; BFU-Es, 70% and 62%; CFU-GEMMs, 70% and 51%; respectively). In most instances CVs were not significantly different from those obtained in the single plate scoring study, suggesting that the scoring process was the most relevant parameter accounting for variations in the reported colony numbers.  (+info)

Role of interleukin 10 and transforming growth factor beta1 in the angiogenesis and metastasis of human prostate primary tumor lines from orthotopic implants in severe combined immunodeficiency mice. (8/3030)

Transfection of primary human prostate tumor cells (i.e., HPCA-10a, 10b, 10c, and 10d lines) with the transforming growth factor (TGF)-beta1 gene stimulated anchorage-independent growth and promoted tumor growth, angiogenesis, and metastasis after orthotopic implantation in severe combined immunodeficiency mice. In contrast, interleukin (IL)-10 transfected cells or cells cotransfected with these two genes exhibited reduced growth rates and significantly reduced angiogenesis and metastasis after 8, 12, and 16 weeks. Enzyme-linked immunosandwich assays confirmed that the respective tumors expressed elevated levels of TGF-beta1 and IL-10 in vivo. ELISAs further showed that TGF-beta1 expression induced matrix metalloproteinases-2 (MMP-2) expression, whereas IL-10 down-regulated MMP-2 expression while up regulating TIMP-1 in the transfected cells. Also, tumor factor VIII levels correlated with TGF-beta1 and MMP-2 expression and inversely with IL-10 and TIMP-1 levels. More importantly, mouse survival was zero after 4-6 months in mice bearing TGF-beta1- and MMP-2-expressing tumors and increased significantly in mice implanted with IL-10- and TIMP-1-expressing tumors (i.e., to >80% survival). Analysis of the metastatic lesions showed that they expressed TGF-beta1 and MMP-2 but barely detectable levels of IL-10 or TIMP-1, suggesting that IL-10 and TIMP-1 might normally block tumor growth, angiogenesis, and metastasis.  (+info)

Colony-Forming Unit-Granulocyte and Monocyte - How is Colony-Forming Unit-Granulocyte and Monocyte abbreviated?Colony-Forming Unit-Granulocyte and Monocyte - How is Colony-Forming Unit-Granulocyte and Monocyte abbreviated?
CFU-GM - Colony-Forming Unit-Granulocyte and Monocyte. Looking for abbreviations of CFU-GM? It is Colony-Forming Unit-Granulocyte and Monocyte. Colony-Forming Unit-Granulocyte and Monocyte listed as CFU-GM
https://acronyms.thefreedictionary.com/Colony-Forming+Unit-Granulocyte+and+Monocyte
JCI - Mechanisms underlying abnormal trafficking of malignant progenitors in chronic myelogenous leukemia. Decreased adhesion...JCI - Mechanisms underlying abnormal trafficking of malignant progenitors in chronic myelogenous leukemia. Decreased adhesion...
We studied the adhesion of primitive and committed progenitors from chronic myelogenous leukemia (CML) and normal bone marrow to stroma and to several extracellular matrix components. In contrast to benign primitive progenitors from CML or normal bone marrow, Ph1-positive primitive progenitors from CML bone marrow fail to adhere to normal stromal layers and to fibronectin and its proteolytic fragments, but do adhere to collagen type IV, an extracellular matrix component of basement membranes. Similarly, multilineage colony-forming unit (CFU-MIX) progenitors from CML bone marrow do not adhere to fibronectin or its adhesion promoting fragments but adhere to collagen type IV. Unlike committed progenitors from normal bone marrow, CML single-lineage burst-forming units-erythroid and granulocyte/macrophage colony-forming units fail to adhere to fibronectin or its components but do adhere to both collagen type IV and laminin. Evaluation of adhesion receptor expression demonstrates that fibronectin ...
https://www.jci.org/articles/view/115985/scanned-page/1235
Plus itPlus it
Antiangiogenic therapy of cancer is highly attractive for several reasons (reviewed in Refs. 38, 39, 40 ). For instance, it can potentially overcome three major problems associated with other anticancer therapies, i.e., the problems of drug resistance (41 , 42) , poor delivery (43 , 44) , and tumor heterogeneity. One approach to the antiangiogenic therapy is antibody-based targeting of tumor vasculature. We have been targeting EDG on tumor vasculature in vivo (9 , 15 , 16) . Several features of EDG are described above (see Introduction). In addition, our anti-EDG mAbs showed little reactivity with normal human bone marrow cells (1) . This observation is consistent with our later finding that anti-EDG immunotoxin (i.e., ricin A-chain conjugate of SN6) selectively eradicated EDG-expressing cells without severely damaging normal human hematopoietic progenitors in the colony-forming unit assays.4 The results suggest that administration of therapeutic doses of an appropriate anti-EDG mAb and ...
http://cancerres.aacrjournals.org/content/61/21/7846
MEDICA
Bone Marrow Cells;Cell Count;Cell Differentiation;Cells, Cultured;Colony-Forming Units Assay;Fetal Blood--cytology;Hematopoietic Stem Cells--cytology; ...
http://digital.library.musc.edu/cdm/search/searchterm/mapping/mode/all/order/creato
MEDICA
Cell Cycle--drug effects;Cells, Cultured;Colony-Forming Units Assay;Hematopoietic Stem Cells--drug effects;Interleukin-3;Fluorouracil;Lymphokines--pharmacology;Mice;Spleen-- ...
http://digital.library.musc.edu/cdm/search/searchterm/5-fluorouracil
Colony-forming units legal definition of colony-forming unitsColony-forming units legal definition of colony-forming units
Definition of colony-forming units in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is colony-forming units? Meaning of colony-forming units as a legal term. What does colony-forming units mean in law?
https://legal-dictionary.thefreedictionary.com/colony-forming+units
Plus itPlus it
We previously demonstrated that 3-azido-3-deoxythymidine (AZT) inhibits growth proliferation of human bone marrow progenitor cells in vitro [Antimicrob. Agents Chemother. 31:452-454 (1987)]. The present study evaluates the effect of toxic concentrations of AZT on possible sites of toxicity in human bone marrow cells. Exposure of cells over a 6-hr period to AZT concentrations between 0.5 and 50 microM resulted in a decreased incorporation of tritiated deoxyguanosine into DNA. Unchanged AZT and its phosphorylated metabolites accumulated within cells after exposure to 10 microM [3H]AZT. 3-Azido-3-deoxythymidine-5-monophosphate was the predominant metabolite, reaching a concentration of 49.2 +/- 14.1 pmol/10(6) cells after 48 hr, and a continuous increase was observed in all phosphorylated derivative levels between 2 and 48 hr of incubation. Using a highly sensitive and specific DNA polymerase assay, endogenous deoxyribonucleotide pool size(s) were analyzed for 48 hr after incubation of cells ...
http://molpharm.aspetjournals.org/content/36/1/9
Endothelial Colony-forming Cells in Hypertension: A Platform of in-vitro Angiogenesis Assessment and Mechanism Discovery -...
We use cookies to ensure that we give you the best experience on our website. If you click Continue well assume that you are happy to receive all cookies and you wont see this message again. Click Find out more for information on how to change your cookie settings ...
https://www.cardioscience.ox.ac.uk/publications/1108477
JRC Publications Repository: In Vitro Myelotoxicity of Environmental Contaminants.JRC Publications Repository: In Vitro Myelotoxicity of Environmental Contaminants.
Myelotoxicity of pesticides and algal toxin was detected in vitro by using the granulocyte-macrophage colony forming unit assay (CFU-GM), and the MTT test with SR-4987 cells, an established stromal cell line derived from a long term murine bone marrow culture, which may represent a suitable in vitro model for studying haematotoxicity. Comparison of the IC50s and NOELs obtained with the CFU-GM assay and those determined by testing the established stromal cells in the MTT cytotoxicity test indicate that inhibition of the proliferation of SR-4987 stromal cells is a sensitive in vitro endpoint for measuring myelotoxicity. It is suggested that this assay could be used as rapid and easy sceening test for determining the haematoxicity of environmental toxins. A comparison with results obtained with the MTT test on a non-differentiated cell line, 3T3-L1, was carried out to distinguish between non-specific interference with cell proliferation and specific toxicity on haemopoietic cells ...
https://publications.jrc.ec.europa.eu/repository/handle/JRC16693
Dilution and Colony-Forming UnitsDilution and Colony-Forming Units
Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted by.
https://brainmass.com/chemistry/physical-chemistry/dilution-colony-forming-units-477874
Endothelial colony-forming cell role in neoangiogenesis and tissue repair<...
TY - JOUR. T1 - Endothelial colony-forming cell role in neoangiogenesis and tissue repair. AU - Critser, Paul J.. AU - Yoder, Mervin C.. PY - 2010/2/1. Y1 - 2010/2/1. N2 - Purpose of review: Patients suffering from vascular disease often have impaired angiogenic ability contributing to impaired tissue repair. One potential therapy is to deliver cells that can aid in angiogenesis. This review will discuss the ability of endothelial progenitor cells (EPCs), which have been reported to contribute to neoangiogenesis in both physiological and pathological conditions, to contribute to neoangiogenesis in tissue repair. Recent findings: In recent years, various reports have described conflicting roles for EPC in vessel formation. Currently there are three different assays for outgrowth of EPC all resulting in the isolation of different cell populations. This confusion is partially due to limited functional characterization of putative EPC populations. One population, endothelial colony-forming cell ...
https://indiana.pure.elsevier.com/en/publications/endothelial-colony-forming-cell-role-in-neoangiogenesis-and-tissu
Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. - Radcliffe Department of...Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. - Radcliffe Department of...
Laminins are alphabetagamma heterotrimeric extracellular proteins that regulate cellular functions by adhesion to integrin and nonintegrin receptors. Laminins containing alpha4 and alpha5 chains are expressed in bone marrow, but their interactions with hematopoietic progenitors are unknown. We studied human bone marrow cell adhesion to laminin-10/11 (alpha5beta1gamma1/alpha5beta2gamma1), laminin-8 (alpha4beta1gamma1), laminin-1 (alpha1beta1gamma1), and fibronectin. About 35% to 40% of CD34(+) and CD34(+)CD38(-) stem and progenitor cells adhered to laminin-10/11, and 45% to 50% adhered to fibronectin, whereas they adhered less to laminin-8 and laminin-1. Adhesion of CD34(+)CD38(-) cells to laminin-10/11 was maximal without integrin activation, whereas adhesion to other proteins was dependent on protein kinase C activation by 12-tetradecanoyl phorbol-13-acetate (TPA). Fluorescence-activated cell-sorting (FACS) analysis showed expression of integrin alpha6 chain on most CD34(+) and CD34(+)CD38(-) cells.
https://www.rdm.ox.ac.uk/publications/736868
Stem cell-like human endothelial progenitors show enhanced colony-forming capacity after brief sevoflurane exposure:...Stem cell-like human endothelial progenitors show enhanced colony-forming capacity after brief sevoflurane exposure:...
BACKGROUND: Endothelial progenitor cells play a pivotal role in tissue repair, and thus are used for cell replacement therapies in regenerative medicine. We tested whether the anesthetic sevoflurane would modulate growth or mobilization of these angiogenic cells. METHODS: In an in vitro model, mononuclear cells isolated from peripheral blood of healthy donors were preconditioned with sevoflurane (3 times 30 min at 2 vol% interspersed by 30 min of air). Colony-forming units were determined after 9 days in culture and compared with time-matched untreated control. Using magnetic cell sorting, CD133+/CD34+ endothelial progenitors were enriched from human umbilical cord blood, and vascular endothelial growth factor (VEGF), VEGFR2 (KDR), granulocyte colony-stimulating factor (G-CSF), STAT3, c-kit, and CXCR4 expressions were determined in sevoflurane-treated and untreated cells by real-time reverse transcriptase polymerase chain reaction. In a volunteer study with crossover design, we tested whether ...
http://www.zora.uzh.ch/id/eprint/24941/
Vasculogenic and osteogenesis-enhancing potential of human umbilical cord blood endothelial colony-forming cells | ScholarBank...Vasculogenic and osteogenesis-enhancing potential of human umbilical cord blood endothelial colony-forming cells | ScholarBank...
Umbilical cord blood-derived endothelial colony-forming cells (UCB-ECFC) show utility in neovascularization, but their contribution to osteogenesis has not been defined. Cocultures of UCB-ECFC with human fetal-mesenchymal stem cells (hfMSC) resulted in earlier induction of alkaline phosphatase (ALP) (Day 7 vs. 10) and increased mineralization (1.9x; p < .001) compared to hfMSC monocultures. This effect was mediated through soluble factors in ECFC-conditioned media, leading to 1.8-2.2x higher ALP levels and a 1.4-1.5x increase in calcium deposition (p < .01) in a dose-dependent manner. Transcriptomic and protein array studies demonstrated high basal levels of osteogenic (BMPs and TGF-βs) and angiogenic (VEGF and angiopoietins) regulators. Comparison of defined UCB and adult peripheral blood ECFC showed higher osteogenic and angiogenic gene expression in UCB-ECFC. Subcutaneous implantation of UCB-ECFC with hfMSC in immunodeficient mice resulted in the formation of chimeric human vessels, ...
http://scholarbank.nus.edu.sg/handle/10635/61664
PDCD2 | Cancer Genetics Web
Programmed cell death-2 (PDCD2) protein is enriched in embryonic, hematopoietic, and neural stem cells, however, its function in stem/progenitor cell differentiation is unclear. We investigated the effects of PDCD2 knockdown on the development and differentiation of hematopoietic progenitor cells (HPC). CD34(+) cells derived from normal human bone marrow and K562 leukemic cells were effectively transduced with short-hairpin RNA to knockdown PDCD2. Colony-forming assays were used to investigate the effects of PDCD2 loss on HPC clonogenic potential and on 12-O-tetradecanoyl-phorbol-13-acetate-and arabinofuranosylcytosine-induced terminal differentiation. In CD34(+) clonogenic progenitors, PDCD2 knockdown decreased the total number of colony-forming units, increased the number of colony-forming units-granulocyte-erythroid-macrophage-megakaryocyte and burst-forming unit-erythroid primitive colonies, and decreased the number of burst-forming unit-erythroid mature colonies. Similar results were ...
http://www.cancerindex.org/geneweb/PDCD2.htm
Plus itPlus it
To expand on the findings from previous clinical trials that the growth of residual tumor is increased at a predictable time following initial drug administration, malignant plasma cells from bone marrows of patients with multiple myeloma (MM) were examined for changes in proliferation and clonogenicity induced in vivo by cyclophosphamide and in vitro by drug-induced humoral stimulatory activity. Peak plasma cell [3H]thymidine labeling index (LI) occurred predictably following drug and paralleled changes in agar colony formation by marrow cells obtained during therapy. Colony-forming capacity of pretreatment MM marrow populations was enhanced when those cells were cultured with humoral stimulatory activity, similar to the increased colony formation detected in Day 9 postcyclophosphamide marrows at the time of peak plasma cell LI.. To further define a relationship between proliferative plasma cells and colony-forming tumor cells, MM Marrows were fractionated by sedimentation on an isokinetic ...
http://cancerres.aacrjournals.org/content/44/9/4197
Volume 6.04 | Feb 1 - Cancer Stem Cell News
Lung Basal Stem Cells Rapidly Repair DNA Damage Using the Error-Prone Nonhomologous End-Joining Pathway Investigators showed that human basal stem cells (BSCs) isolated from heavy smokers proliferate extensively, whereas their alveolar progenitor cell counterparts have limited colony-forming capacity. They demonstrated that this difference arises in part because of the ability of BSCs to repair their DNA more efficiently than alveolar cells following ionizing radiation or chemical-induced DNA damage. [PLoS Biol] Full Article , Press Release Differentiated State of Initiating Tumor Cells Is Key to Distinctive Immune Responses Seen in H-RasG12V-Induced Squamous Tumors In two doxycycline-inducible models where oncogenic H-RasG12V is targeted either to the epidermal basal/stem cell layer with a Keratin14-rtTA transgene, or committed progenitor/suprabasal cells with an Involucrin-tTA transgene, investigators observed strikingly distinct tumor immune responses. These data showed that position of tumor ...
http://www.cancerstemcellnews.com/issue/volume-6-04-feb-1/
Effects of 1R-Chl on colony formation of CML patient MN | Open-iEffects of 1R-Chl on colony formation of CML patient MN | Open-i
Effects of 1R-Chl on colony formation of CML patient MNCs and normal human MNCs.(A) MNCs from CML patients were cultured with rh stem cell factor, rh IL-3, rh G
https://openi.nlm.nih.gov/detailedresult.php?img=PMC2571993_pone.0003593.g002&req=4
Volume 4.00 | Jan 5 - Cord Blood News
Vascular Incorporation of Endothelial Colony-Forming Cells Is Essential for Functional Recovery of Murine Ischemic Tissue following Cell Therapy
https://www.cordbloodresearchnews.com/issue/volume-4-00-jan-5/
SmartDish™ Meniscus-Free Culture Plates for CFU Assays | STEMCELL TechnologiesSmartDish™ Meniscus-Free Culture Plates for CFU Assays | STEMCELL Technologies
SmartDish™ is meniscus-free cultureware that improves the accuracy of manual and automated counting of hematopoietic colony assays.
https://www.stemcell.com/product-portfolios/hematopoietic-stem-and-progenitor-cell-research/analysis/smartdish.html
SmartDish™ Meniscus-Free Culture Plates for CFU Assays | STEMCELL TechnologiesSmartDish™ Meniscus-Free Culture Plates for CFU Assays | STEMCELL Technologies
SmartDish™ is meniscus-free cultureware that improves the accuracy of manual and automated counting of hematopoietic colony assays.
https://cdn.stemcell.com/products/smartdish.html
SmartDish™ | STEMCELL TechnologiesSmartDish™ | STEMCELL Technologies
SmartDish™ is meniscus-free cultureware that improves the accuracy of manual and automated counting of hematopoietic colony assays.
https://www.stemcell.com/products/product-types/cultureware-and-general-supplies/smartdish.html
Open Doors: Accessing Government Records - Society of Professional JournalistsOpen Doors: Accessing Government Records - Society of Professional Journalists
The Society of Professional Journalists is the nations most broad-based journalism organization, dedicated to encouraging the free practice of journalism and stimulating high standards of ethical behavior.
https://www.spj.org/opendoors5.asp
Uridine reverses the toxicity of 3-azido-3-deoxythymidine in normal human granulocyte-macrophage progenitor cells in vitro...Uridine reverses the toxicity of 3-azido-3-deoxythymidine in normal human granulocyte-macrophage progenitor cells in vitro...
We evaluated the effects of natural purine and pyrimidine nucleosides on protection from or reversal of 3-azido-3-deoxythymidine (AZT) cytotoxicity in human bone marrow progenitor cells by using clonogenic assays. The selectivity of the protection or rescue agents was examined in evaluating the antiretroviral activity of AZT in combination with these modulating agents and of AZT alone. Following exposure of human granulocyte-macrophage progenitor cells for 2 h to 5 microM AZT (70% inhibitory concentration), increasing concentrations of potential rescue agents were added. Cells were cultured, and colony formation was assessed after 14 days. At concentrations of up to 50 microM no natural 2-deoxynucleosides, including thymidine, were able to reverse the toxic effects of AZT. Dose-dependent reversal was observed with uridine and cytidine, and essentially complete reversal was achieved with 50 microM uridine. In the protection studies, 100 microM thymidine almost completely antagonized the ...
https://aac.asm.org/content/32/7/997
CD4 is expressed on murine pluripotent hematopoietic stem cells | Blood | American Society of HematologyCD4 is expressed on murine pluripotent hematopoietic stem cells | Blood | American Society of Hematology
Abstract. We show here for the first time that pluripotent hematopoietic stem cells express the CD4 antigen. CD4+ cells isolated from mouse marrow repopulated
https://ashpublications.org/blood/article/80/7/1717/169494/CD4-is-expressed-on-murine-pluripotent
Intrinsic Hematopoietic Stem Cell/Progenitor Plasticity: Inversions<...
TY - JOUR. T1 - Intrinsic Hematopoietic Stem Cell/Progenitor Plasticity. T2 - Inversions. AU - Colvin, Gerald A.. AU - Lambert, Jean François. AU - Moore, Brian E.. AU - Carlson, Jane E.. AU - Dooner, Mark S.. AU - Abedi, Mehrdad. AU - Cerny, Jan. AU - Quesenberry, Peter J.. PY - 2004/4. Y1 - 2004/4. N2 - Traditional concepts indicate that stem cells give rise to progenitor cells in a hierarchical system. We studied murine engraftable stem cells (ESCs) and progenitors in in vitro and found that ESC and progenitors exist in a reversible continuum, rather then a hierarchy. B6.SJL and BALB/c marrow cells were serially cultured with thrombopoietin (TPO), FLT-3 ligand (FLT-3L), and steel factor through cell cycle. Progenitors (high-proliferative potential colony-forming cells (HPP-CFC) and colony-forming unit culture (CFU-c)) and ESC capacity was determined. The cell cycle status of purified lineage negativerhodaminelowHoechstlow stem cells was determined under the same conditions using tritiated ...
https://ucdavis.pure.elsevier.com/en/publications/intrinsic-hematopoietic-stem-cellprogenitor-plasticity-inversions
L-selectin - wikidocL-selectin - wikidoc
L-selectin acts as a homing receptor for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.[2] The receptor is commonly found on the cell surfaces of T cells. Naive T-lymphocytes, which have not yet encountered their specific antigen, need to enter secondary lymph nodes to encounter their antigen. Central memory T-lymphocytes, which have encountered antigen, express L-selectin to localize in secondary lymphoid organs. Here they reside ready to proliferate upon re-encountering antigen. Effector memory T-lymphocytes do not express L-selectin, as they circulate in the periphery and have immediate effector functions upon encountering antigen. High expression of L-selectin on human bone marrow progenitor cells is an early sign of cells becoming committed to lymphoid ...
https://www.wikidoc.org/index.php/L-selectin
Down regulation of stem cell colony formation by purified CD8 lymphocytes and CD8 conditioned medium: potential importance for...Down regulation of stem cell colony formation by purified CD8 lymphocytes and CD8 conditioned medium: potential importance for...
A methodology for selection of the CD8 cell subset from the peripheral blood and bone marrow mononuclear cells was developed using anti-T8 (CD8) antibody and magnetic microspheres coated with anti-mouse IgG. Following optimization of antibody:cell binding ratio and microsphere:cell ratios, CD8(+)-cells in the peripheral blood and bone marrow were effectively removed, with an overall final recovery of 34.9% +/- 8.6%, and 56% +/- 8.5% respectively with complete recovery of stem cells and very little contamination with effector cells. CD8(+)-depleted cell preparations demonstrated a 3-4 fold increase in CFU-C and CFU-E colony formation over non-depleted preparations when stimulated with G-CSF, GM-CSF or IL-3 and erythropoietin. The largest increase in colony formation was evident when IL-3 was used to stimulate colony formation. Purified autologous CD8+ T-cells or culture supernatant from in vitro cultures of purified autologous CD8+ T-cells added back to CD8 depleted preparations, induced 20%-90%
https://www.semanticscholar.org/paper/Down-regulation-of-stem-cell-colony-formation-by-p-Gazitt-He/036a6b8f5086a7f72d736bcdf962a242768f089a
The same exhaustible multilineage precursor produces both myeloid and by D E. Harrison and R K. ZhongThe same exhaustible multilineage precursor produces both myeloid and by D E. Harrison and R K. Zhong
Hemopoietic precursors with the ability to differentiate into wide varieties of cell types are considered primitive, as are precursors with long-term repopulating ability. Here we study the populations of marrow precursors from which both myeloid and lymphoid lineages are descended shortly after transplantation. Surprisingly, few or none of these precursors show long-term repopulating ability. Equal portions of a mixture of marrow cells from C57BL/6J (B6) and congenic B6-Hbbd Gpi-1a mice are transplanted into a group of recipients. Three weeks later, highly significant correlations between percentages of B6 type T cells, B cells, granulocytes, and platelets in each recipient indicate that many lymphoid and myeloid cells are descended from common precursors. After 4-6 weeks, most correlations between lymphoid and myeloid cells improve, indicating that most or all differentiated cells are descended from common precursors. The more differentiated myeloid-specific precursors found in spleen
https://mouseion.jax.org/stfb1990_1999/311/
Differentiation of Neural-Crest-Derived Intermediate Pluripotent Progenitors into Committed Periodontal Populations Involves...Differentiation of Neural-Crest-Derived Intermediate Pluripotent Progenitors into Committed Periodontal Populations Involves...
Differentiation of Neural-Crest-Derived Intermediate Pluripotent Progenitors into Committed Periodontal Populations Involves Unique Molecular Signature Changes, Cohort Shifts, and Epigenetic Modifications. Smit Jayant Dangaria, Yoshihiro Ito, Xianghong Luan, Thomas G.H. Diekwisch. Stem Cells Dev. 2011 January; 20(1): 39-52. Published online 2010 July 6. doi: 10.1089/scd.2010.0180. PMCID: PMC3128775 ...
http://www.omicsoft.com/publications/2014/2/22/differentiation-of-neural-crest-derived-intermediate-pluripotent-progenitors-into-committed-periodontal-populations-involves-unique-molecular-signature-changes-cohort-shifts-and-
91206 PRIME-XV® Mouse Hematopoietic Cell Basal Medium | Irvine Scientific91206 PRIME-XV® Mouse Hematopoietic Cell Basal Medium | Irvine Scientific
PRIME-XV® Mouse Hematopoietic Cell Basal Medium was developed in a collaboration program with a leading stem-cell research facility in the USA to fulfill the need for a serum-free, cost-effective medium for in vitro culture and expansion and self-renewal of murine hematopoietic progenitor cells (mHPCs).. PRIME-XV Mouse Hematopoietic Cell Basal Medium is:. ...
http://www.irvinesci.com/products/91206-prime-xv-mouse-hematopoietic-cell-basal-medium?dpt=Cell+Therapy
2016 January | microrna inhibitor
Confluent monolayers of LLC-MK2 cells used in FFU reduction assays were exposed to increasing concentrations of peptide before measuring selleck chemicals Tofacitinib mitochondrial reductase activity using an MTT mitochondrial reductase activity assay (Figure 3). When we initially performed these assays to exactly mimic the focus forming unit assay by waiting five days after peptide exposure, we saw no evidence of toxicity at any concentration of any peptide (data not shown). However, we found that a shorter post-exposure incubation time revealed a subtle toxicity on the part of one of the peptides. Apparently, waiting more than 24 h post-exposure gives the cells a chance to recover and conceals this effect. At 24 h post-exposure, DN57opt was found to be mildly toxic to cells at 40 ��M (one-way ANOVA with Dunnets post hoc test, P=0.. 0004, N=18), so only inhibitory data using lower, nontoxic concentrations was considered. Peptides DN57optscr, 1OAN1, and 1OAN1scr were not toxic at any ...
http://micrornainhibitor.com/2016/01
Stem Cell Assay Market: Global Industry Analysis, Size, Share, Growth, Trends, and Forecasts 2016&ndStem Cell Assay Market: Global Industry Analysis, Size, Share, Growth, Trends, and Forecasts 2016&nd
Stem cells are distinctive cells that can differentiate into any form of the cell of the body owing to its property of totipotency. Hence, they are a continuous resource of the specialized cells that built the organs and tissues.
http://www.sbwire.com/press-releases/stem-cell-assay-market/release-764159.htm
Services - Tissue Culture ServicesServices - Tissue Culture Services
Caltag Medsystems offer the following services: custom antibodies, angiogenesis services, drug discovery, stem cell assays, genotyping, custom MHC tetramers, flow cytometry, immunoassays, medical device testing and protein analysis.
https://www.caltagmedsystems.co.uk/pricing_ordering/index.php?PT_ID=11&PG_ID=73
Services - ELISAServices - ELISA
Caltag Medsystems offer the following services: custom antibodies, angiogenesis services, drug discovery, stem cell assays, genotyping, custom MHC tetramers, flow cytometry, immunoassays, medical device testing and protein analysis.
https://www.caltagmedsystems.co.uk/pricing_ordering/index.php?PT_ID=11&PG_ID=76
Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain<...
TY - JOUR. T1 - Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain. AU - Spivak, J. L.. AU - Misiti, J.. AU - Stuart, R.. AU - Sharkis, S. J.. AU - Sensenbrenner, L. L.. PY - 1980. Y1 - 1980. N2 - Clonal assays for CFU-S, CFU-C, BFU-E, and CFU-E were employed to evaluate the effect of ouabain on the proliferation of mouse hematopoietic progenitor cells. Preincubation of bone marrow cells with ouabain at concentrations of 10-6-10-12 M suppressed the proliferation of CFU-S as measured by the spleen colony assay. At 10-9 M ouabain, spleen colony formation was inhibited by more than 95%. When included in soft agar cultures of bone marrow cells, ouabain suppressed the proliferation of CFU-C in a complex fashion. At 10-4 M ouabain, colony formation was suppressed by 70%, while at 10-6 M ouabain, CFU-C proliferation was normal. At 10-9 M ouabain, however, the number of colonies formed was only 70% of normal, and complete recovery was not obtained at 10-12 M ...
https://jhu.pure.elsevier.com/en/publications/suppression-and-potentiation-of-mouse-hematopoietic-progenitor-ce-3
Ovine haemopoiesis: the development of bone marrow-derived colony-forming cells in vitro in the presence of factors derived...Ovine haemopoiesis: the development of bone marrow-derived colony-forming cells in vitro in the presence of factors derived...
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of normal mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
https://www.semanticscholar.org/paper/Ovine-haemopoiesis-the-development-of-bone-marrow-Haig-Brown/48b4d5454b77f06844deb9d0eade0e928eaacaf3
Association of cell cycle expression of Ia-like antigenic determinants on normal human multipotential (CFU-GEMM) and erythroid ...
TY - JOUR. T1 - Association of cell cycle expression of Ia-like antigenic determinants on normal human multipotential (CFU-GEMM) and erythroid (BFU-E) progenitor cells with regulation in vitro by acidic isoferritins. AU - Lu, L.. AU - Broxmeyer, H. E.. AU - Meyers, P. A.. AU - Moore, M. A.. AU - Thaler, H. T.. PY - 1983. Y1 - 1983. N2 - An association has been established between human Ia-like antigenic determinants, expression during DNA synthesis on multipotential (CFU-GEMM) and erythroid (BFU-E) progenitor cells, and the regulatory action of acidic isoferritins in vitro. Treatment of human bone marrow cells with monoclonal anti-Ia (NE1-011) plus complement inhibited colony formation of CFU-GEMM and BFU-E by 50%-70%. Reduction of colonies was similar whether bone marrow cells were exposed to anti-Ia plus complement, high specific activity tritiated thymidine (3HTdr), or acidic isoferritins. No further decrease was apparent with 3HTdr or acidic isoferritins after Ia-antigen+ CFU-GEMM or BFU-E ...
https://indiana.pure.elsevier.com/en/publications/association-of-cell-cycle-expression-of-ia-like-antigenic-determi
Repositório Aberto da Universidade do Porto: Thymic crosstalk restrains the pool of cortical thymic epithelial cells with...Repositório Aberto da Universidade do Porto: Thymic crosstalk restrains the pool of cortical thymic epithelial cells with...
Cortical (cTEC) and medullary (mTEC) thymic epithelial cells establish key microenvironments for T-cell differentiation and arise from thymic epithelial cell progenitors (TEP). However, the nature of TEPs and the mechanism controlling their stemness in the postnatal thymus remain poorly defined. Using TEC clonogenic assays as a surrogate to survey TEP activity, we found that a fraction of cTECs generates specialized clonal-derived colonies, which contain cells with sustained colony-forming capacity (ClonoTECs). These ClonoTECs are EpCAM+MHCII-Foxn1lo cells that lack traits of mature cTECs or mTECs but co-express stem-cell markers, including CD24 and Sca-1. Supportive of their progenitor identity, ClonoTECs reintegrate within native thymic microenvironments and generate cTECs or mTECs in vivo. Strikingly, the frequency of cTECs with the potential to generate ClonoTECs wanes between the postnatal and young adult immunocompetent thymus, but it is sustained in alymphoid Rag2-/-Il2rg-/- counterparts. ...
https://repositorio-aberto.up.pt/handle/10216/111812?mode=full
Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis<...Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis<...
TY - JOUR. T1 - Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis. AU - Osborn, Mark J.. AU - Ryan, Patricia L.. AU - Kirchhof, Nicole. AU - Panoskaltsis-Mortari, Angela. AU - Mortari, Frank. AU - Tudor, Kim Sue R S. PY - 2004/2/1. Y1 - 2004/2/1. N2 - The role of thymic stromal cell-derived lymphopoietin (TSLP) in regulating hematopoiesis is poorly characterized, so we investigated its regulatory effects in vivo using TSLP transgenic mice. Overexpression of TSLP disrupted hematopoietic homeostasis by causing imbalances in lymphopoiesis and myelopoiesis. Mice harboring a TSLP transgene had 5- to 700-fold fewer B and T precursors and no detectable pre-B lymphocyte colony-forming activity in the marrow or spleen. Conversely, TSLP transgenic mice possessed 15 to 20 times more spienic myeloid precursors than their littermates, and progenitor activity of the granulocyte-erythrocyte-macrophage-megakaryocyte colony-forming units was significantly elevated. The arrest in lymphopoiesis ...
https://experts.umn.edu/en/publications/overexpression-of-murine-tslp-impairs-lymphopoiesis-and-myelopoie
Brief Report: The lincRNA Hotair Is Required for Epithelial-to-Mesenchymal Transition and Stemness Maintenance of Cancer Cell...Brief Report: The lincRNA Hotair Is Required for Epithelial-to-Mesenchymal Transition and Stemness Maintenance of Cancer Cell...
Hotair is a member of the recently described class of noncoding RNAs called lincRNA (large intergenic noncoding RNA). Various studies suggest that Hotair acts regulating epigenetic states by recruiting chromatin-modifying complexes to specific target sequences that ultimately leads to suppression of several genes. Although Hotair has been associated with metastasis and poor prognosis in different tumor types, a deep characterization of its functions in cancer is still needed. Here, we investigated the role of Hotair in the scenario of epithelial-to-mesenchymal transition (EMT) and in the arising and maintenance of cancer stem cells (CSCs). We found that treatment with TGF-1 resulted in increased Hotair expression and triggered the EMT program. Interestingly, ablation of Hotair expression by siRNA prevented the EMT program stimulated by TGF-1, and also the colony-forming capacity of colon and breast cancer cells. Furthermore, we observed that the colon CSC subpopulation (CD133(+)/CD44(+)) ...
https://repositorio.unesp.br/handle/11449/112059
Functional p85α gene is required for normal murine fetal erythropoiesis<...
TY - JOUR. T1 - Functional p85α gene is required for normal murine fetal erythropoiesis. AU - Huddleston, Hannah. AU - Tan, Bailin. AU - Yang, Feng Chun. AU - White, Hilary. AU - Wenning, Mary Jo. AU - Orazi, Attilio. AU - Yoder, Mervin C.. AU - Kapur, Reuben. AU - Ingram, David A.. PY - 2003/7/1. Y1 - 2003/7/1. N2 - In vitro studies suggest that activation of class IA phosphatidyiinositol 3 (PI-3) kinase is necessary for normal erythroid cell development. However, when class IA PI-3 kinase-deficient mice were generated by a targeted deletion of the p85α regulatory subunit, fetal erythropoiesis was reportedly unaffected. Given the discrepancies between these studies, we performed a more detailed in vivo analysis of class IA PI-3 kinase-deficient embryos. Day-14.5 p85α-/- embryos are pale with a marked reduction of mature erythrocytes in their peripheral blood. Further, the absolute number and frequency of both early (erythroid burst-forming unit [BFU-E]) and late erythroid progenitors ...
https://miami.pure.elsevier.com/en/publications/functional-p85%CE%B1-gene-is-required-for-normal-murine-fetal-erythrop
Release of Colony-Stimulating Activity from the Isolated Perfused Rat Liver | Clinical Science | Portland PressRelease of Colony-Stimulating Activity from the Isolated Perfused Rat Liver | Clinical Science | Portland Press
1. Colony-stimulating activity appeared in the perfusate of the isolated rat liver during perfusions with either whole rat blood, rat plasma or an artificial perfusate of Eagles medium and albumin.. 2. Dialysable inhibitors of colony formation were also released during perfusions.. 3. Colony-stimulating activity in artificial perfusate could be enhanced by the addition of rat plasma in vitro. Concentrations of cycloheximide that inhibited albumin synthesis by the liver did not inhibit the release of colony-stimulating activity. ...
https://portlandpress.com/clinsci/article-abstract/50/6/539/70480/Release-of-Colony-Stimulating-Activity-from-the?redirectedFrom=fulltext
The global stem cell assay market forecast from 2016 to 2021 insights shared in detailed report - WhaTechThe global stem cell assay market forecast from 2016 to 2021 insights shared in detailed report - WhaTech
Stem Cell Assay Market By Assay (Cell Viability, Cell Differentiation, Cell Identification), By Technology, By Kit (Adult Stem Cell Kits, Human Embryonic Stem Cells Kits), By Product (Instruments, Detection Kits), By Application (Regenerative Medicines, Drug Development And Clinical Research), By End User (Research Institutes And Industry Research) And By Region - Global Industry Analysis, Size, Share, Growth, Trends, And Forecasts (2016-2021)
https://www.whatech.com/market-research/medical/388280-the-global-stem-cell-assay-market-forecast-from-2016-to-2021-insights-shared-in-detailed-report
Development and aging of primitive hematopoietic stem cells in BALB/c by J Chen, C M. Astle et al.Development and aging of primitive hematopoietic stem cells in BALB/c by J Chen, C M. Astle et al.
Evaluating the function of an individual hematopoietic stem cell (HSC) is a difficult and important problem. The functional ability per HSC, as well as the HSC concentration, was measured with minimal disruption to the cells in vivo using the new competitive dilution assay. Distribution of HSC into recipients was modeled based on Poisson probabilities. Predictions of donor contributions from models assuming different levels of donor HSC functional ability and concentration were compared to actual observations. The model with the least difference between predictions and observations was accepted. In BALB/ cBy (BALB) mice, models assuming equal functional ability of HSC from the same donor fit extremely well with actual observations, suggesting that all HSC are functionally homogeneous at any particular time point during development or aging. Relative HSC functional ability per cell declined during development, so that a fetal HSC had 1.6 to 3.0 times the functional ability of a young adult
https://mouseion.jax.org/stfb1990_1999/1218/
AABB Accredited Hematopoietic Progenitor Cell (HPC) FacilitiesAABB Accredited Hematopoietic Progenitor Cell (HPC) Facilities
AABB Hematopoietic Progenitor Cell (HPC) activities include educational programs, publications and accreditation for HPC programs.. The list of AABB Accredited HPC Facilities specifies those HPC facilities, in the US and throughout the world, which have attained AABB accreditation. These facilities are responsible for procuring, processing and storing hematopoietic progenitor cells that can be used for transplantation.. ...
http://www.aabb.org/SA/FACILITIES/CELLTHERAPY/Pages/HPCAccrFac.aspx
Genes to cells - Research database - University of GroningenGenes to cells - Research database - University of Groningen
Zeisberger, S. M., Zoller, S., Riegel, M., Chen, S., Krenning, G., Harmsen, M. C., ... Zisch, A. H. (2010). Optimization of the culturing conditions of human umbilical cord blood-derived endothelial colony-forming cells under xeno-free conditions applying a transcriptomic approach. Genes to cells, 15(7), 671-687. https://doi.org/10.1111/j.1365-2443.2010.01409.x ...
https://www.rug.nl/research/portal/journals/genes-to-cells
JCI - Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cellsJCI - Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
Downloading a figure as powerpoint requires a browser with javascript support. Enable javascript and try again For help please contact [email protected] ...
https://www.jci.org/assets/348764/powerpoint
JCI - Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cellsJCI - Targeting VLA4 integrin and CXCR2 mobilizes serially repopulating hematopoietic stem cells
Downloading a figure as powerpoint requires a browser with javascript support. Enable javascript and try again For help please contact [email protected] ...
https://m.jci.org/assets/348768/powerpoint
Levels of circulating endothelial cells and colony-forming units are influenced by age and dyslipidemia.
The balance between endothelial injury and repair in childhood is poorly understood. We examined this relationship in healthy children, in adults, and in children with familial hypercholesterolemia (FH). Circulating endothelial cells (CECs) wer
http://www.biomedsearch.com/nih/Levels-circulating-endothelial-cells-colony/22785446.html
Effect of GPC3 on cell proliferation and clonogenic cap | Open-iEffect of GPC3 on cell proliferation and clonogenic cap | Open-i
Effect of GPC3 on cell proliferation and clonogenic capacity of liver CD90+GPC3+CSCs.(A) Cell proliferation was assessed after GPC3 knockdown in PLC CD90+GPC3+
https://openi.nlm.nih.gov/detailedresult.php?img=PMC3351419_pone.0037159.g009&req=4
Ex Vivo-Expanded HER2-Specific T Cells and Cyclophosphamide After Vaccine Therapy in Treating Patients With HER2-Positive Stage...Ex Vivo-Expanded HER2-Specific T Cells and Cyclophosphamide After Vaccine Therapy in Treating Patients With HER2-Positive Stage...
RATIONALE : Laboratory-treated T cells may stimulate the immune system in different ways and stop tumor cells from growing. Drugs used in chemotherapy,
http://www.knowcancer.com/cancer-trials/NCT01219907/
Clonogenic Assay
Clonogenic assay allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate. - Clonogenic Assay - AbVideo™ - Support - Abnova
http://www.abnova.com/abvideo/Clonogenic-Assay.html
Protocols and Video Articles Authored by Chitra VenugopalProtocols and Video Articles Authored by Chitra Venugopal
Chitra Venugopal is the author of this article in the Journal of Visualized Experiments: Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
https://www.jove.com/author/Chitra_Venugopal
Why Is There Antibiotic in Cell Culture Media? | ZymergiWhy Is There Antibiotic in Cell Culture Media? | Zymergi
The second problem with antibiotics is that they interfere with detection. QC Microbiology tells us their tests are sensitive to 1 colony-forming unit (CFU) in the sample, which means if there is 1 CFU in a 40mL sample bottle, theyll find it. But 1 CFU/40mL is 25 CFU/L... and for a 12,000L bioreactor, you need a contamination of ~300,000 CFUs in order for QC to detect contamination ...
http://blog.zymergi.com/2013/07/why-is-there-antibiotic-in-cell-culture.html
thymodepressin Summary Report | CureHunterthymodepressin Summary Report | CureHunter
thymodepressin: a synthetic peptide, promoted take of transplanted hemopoietic precursor cells in the bone marrow of irradiated mice
http://www.curehunter.com/public/keywordSummaryC449663-thymodepressin.do
hemopoiesis - ဝစ်ရှင်နရီhemopoiesis - ဝစ်ရှင်နရီ
စာသားများကို Creative Commons Attribution-ShareAlike လိုင်စင်ဖြင့် ရရှိနိုင်ပြီး ထပ်ဆောင်းသတ်မှတ်ချက်များ ရှိနိုင်သည်။ အသေးစိတ်အတွက် အသုံးပြုခြင်းဆိုင်ရာ သတ်မှတ်ချက်များတွင် ကြည့်ပ ...
https://my.wiktionary.org/wiki/hemopoiesis
Mesenchymal stem cellMesenchymal stem cell
In this assay system, stromal cells were referred to as colony-forming unit-fibroblasts (CFU-f). The first clinical trials of ... The majority of modern culture techniques still take a colony-forming unit-fibroblasts (CFU-F) approach, where raw unpurified ... Siminovitch L, Mcculloch EA, Till JE (December 1963). "The distribution of colony-forming cells among spleen colonies". Journal ... "Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method". ...
https://en.wikipedia.org/wiki/Mesenchymal_stem_cell
Stemcell TechnologiesStemcell Technologies
... is a methylcellulose-based medium commonly used in hematopoietic colony-forming unit (CFU) assays. STEMCELL's mTeSR™1 medium is ... The company also markets education, custom manufacturing, and contract assay services for academic and industrial scientists. ...
https://en.wikipedia.org/wiki/Stemcell_Technologies
Colony-forming unitColony-forming unit
"Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: comparison with manual ... In microbiology, colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi ... Instead of colony-forming units, the parameters Most Probable Number (MPN) and Modified Fishman Units (MFU)[citation needed] ... it is uncertain if the colony arose from one cell or a group of cells. Expressing results as colony-forming units reflects this ...
https://en.wikipedia.org/wiki/Colony-forming_unit
List of MeSH codes (E05)List of MeSH codes (E05)
... colony-forming units assay MeSH E05.200.500.383.910 - tumor stem cell assay MeSH E05.200.500.385 - cytogenetic analysis MeSH ... drug screening assays, antitumor MeSH E05.337.550.200.800 - tumor stem cell assay MeSH E05.337.550.200.900 - xenograft model ... drug screening assays, antitumor MeSH E05.200.500.388.930 - tumor stem cell assay MeSH E05.200.500.410 - electroporation MeSH ... antitumor assays MeSH E05.337.550.200.900.830 - subrenal capsule assay MeSH E05.337.550.400 - microbial sensitivity tests MeSH ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)
CFU-ECFU-E
... colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue ... CFU-E stands for Colony Forming Unit-Erythroid. It arises from CFU-GEMM (via BFU-E, which stands for "erythroid burst-forming ... Marley SB, Lewis JL, Goldman JM, Gordon MY (June 1996). "Abnormal kinetics of colony formation by erythroid burst-forming units ... CFU-E at the US National Library of Medicine Medical Subject Headings (MeSH) (Blood cells, Colony forming units). ...
https://en.wikipedia.org/wiki/CFU-E
BioluminescenceBioluminescence
There is a direct correlation between bioluminescence and colony forming unit (CFU). Thus, concentration of standard ATP and ... Renilla and Gaussia based cell viability assays use the substrate coelenterazine. Animal coloration Biophoton Life That Glows, ... and even to light from other nearby colonies. Communication by light emission between the zooids enables coordination of colony ... Communication in the form of quorum sensing plays a role in the regulation of luminescence in many species of bacteria. Small ...
https://en.wikipedia.org/wiki/Bioluminescence
Titanocene YTitanocene Y
"Antiproliferative activity of Titanocene Y against tumor colony-forming units". Anticancer Drugs. 18 (3): 317-321. doi:10.1097/ ... "Analyses of Titanocenes in the spheroid-based cellular angiogenesis assay". Toxicol in Vitro. 22 (2): 531-534. doi:10.1016/j. ... Titanocene Y can be given in the mouse in high dosages and it shows generally mild toxicity in the form of diarrhea. Titanocene ...
https://en.wikipedia.org/wiki/Titanocene_Y
LegionellaLegionella
The working group has published guidelines about the actions to be taken to limit the number of colony-forming units (that is, ... Protection was dose-dependent and correlated with antibody levels as measured by enzyme-linked immunosorbent assay to an outer ... Kadaifciler DG, Cotuk A (June 2014). "Microbial contamination of dental unit waterlines and effect on quality of indoor air". ... A biomonitoring solution exists in a Legionella-specific aptamer-based assay. Control of Legionella growth can occur through ...
https://en.wikipedia.org/wiki/Legionella
Virtual colony countVirtual colony count
Virtual colony forming units, or CFUv, is defined in the original VCC publication and its definition is repeated here: CFUv was ... Virtual colony count (VCC) is a kinetic, 96-well microbiological assay originally developed to measure the activity of ... After spreading, the agar plates are then incubated overnight and the number of colony forming units (CFU) is counted the next ... Because this procedure requires no actual colony formation or colony counting, it is termed "virtual colony count". Thus far ...
https://en.wikipedia.org/wiki/Virtual_colony_count
Minimum inhibitory concentrationMinimum inhibitory concentration
The microbe, or the inoculating agent, must come from the same colony-forming unit, and must be at the correct concentration. ... There are three main reagents necessary to run this assay: the media, an antimicrobial agent, and the microbe being tested. The ... For verification, the positive control is plated in a hundred fold dilution to count colony forming units. The microbes ... Antimicrobial toxicity can come in many forms, such as immune hypersensitivity and off-target toxicity. ...
https://en.wikipedia.org/wiki/Minimum_inhibitory_concentration
Index of biochemistry articlesIndex of biochemistry articles
... granulocyte colony-stimulating factor - granulocyte colony-stimulating factor receptor - granulocyte-macrophage colony- ... atomic mass unit - atomic nucleus - atomic number - atomic orbital - atomic radius - Atomic weight - ATP synthase - ATPase - ... kinetic exclusion assay - kinetics - knock-out mouse - Krebs cycle lactalbumin - lactic acid - lactic acid autotroph - lactic ... life form - ligand - light reactions - Lineweaver-Burke-diagram - lipase - lipid - lipid anchored protein - lipid bilayer - ...
https://en.wikipedia.org/wiki/Index_of_biochemistry_articles
Clostridium butyricumClostridium butyricum
106 colony forming units (CFU) of C. butyricum MIYAIRI 588 (as active agent). CBM 588 does not establish permanently in the gut ... has been demonstrated by PCR assay. This strain is deposited at the Fermentation Research Institute, Agency of Industrial ... Clostridium butyricum is a strictly anaerobic endospore-forming Gram-positive butyric acid-producing bacillus subsisting by ...
https://en.wikipedia.org/wiki/Clostridium_butyricum
Cell countingCell counting
... it can be generally assumed that each cell will give rise to a single colony or Colony Forming Unit (CFU). The colonies can ... for instance the Luria-Delbrück experiment or the gentamicin protection assay). In addition, the enumeration of colonies on ... instead of obtaining single colonies that can be counted, a so-called "lawn" will form: thousands of colonies lying over each ... Additionally, plating is the slowest method of all: most microorganisms need at least 12 hours to form visible colonies. ...
https://en.wikipedia.org/wiki/Cell_counting
Campylobacter coliCampylobacter coli
The infectious dose of Campylobacter ranges between 1000-10,000, but even 500-800 colony forming units (CFU) has also been ... can be diagnosed by isolation of the organism from faeces and identification by growth-dependent tests, immunological assays, ... In the year 1886, Theodor Escherich established that spiral form bacteria were noted in the stool specimen and large intestinal ... Campylobacter coli is a Gram-negative, microaerophilic, non-endospore-forming, S-shaped bacterial species within genus ...
https://en.wikipedia.org/wiki/Campylobacter_coli
Indoor bioaerosolIndoor bioaerosol
... in which colony forming units (CFU) on selective media are counted. Cultivating methods have several disadvantages. Culture- ... enzyme-linked immunosorbent assay (ELISA)). The well-known PCR is a powerful tool in identifying and even quantifying the ... This underestimation is likely to be signified for the quantification of bioaerosol, since colony counts of airborne microbes ... irrelevance of sampling units to human exposure measurement; multiplicity and variability of composition, etc.). To enable ...
https://en.wikipedia.org/wiki/Indoor_bioaerosol
Rathayibacter toxicusRathayibacter toxicus
Pure yellow colonies formed within 24 hours. The identification of the bacterium as Corynebacterium rathayi was insufficiently ... A functional tunicamycin gene cluster has been identified consisting of 14 genes composing 2 separate transcriptional units. ... were distinguishable from not only Corynebacterium rathayi but other phytopathogenic coryneforms through immunological assays. ... Colony morphology on 523M agar is convex, smooth, mucoid with yellow, rose-orange, or pink pigmentation. Anguina sp. (seed gall ...
https://en.wikipedia.org/wiki/Rathayibacter_toxicus
List of Legionnaires disease outbreaksList of Legionnaires' disease outbreaks
This group has published guidelines about the actions to be taken to limit the number of colony forming units (i.e., the " ... disease by serological assays". BMC Infectious Diseases. 15: 163. doi:10.1186/s12879-015-0903-2. PMC 4383209. PMID 25887275. ... The tabled figures are for total aerobic plate count, cfu/ml at 30 °C (minimum 48 hours incubation) with colony count ... of freshwater sites by PCR hybridization assay.[citation needed] Legionella bacteria themselves can be inactivated by UV light ...
https://en.wikipedia.org/wiki/List_of_Legionnaires'_disease_outbreaks
AcinetobacterAcinetobacter
The other reliable identification test at genus level is chromosomal DNA transformation assay. In this assay, a naturally ... Acinetobacter is frequently isolated in nosocomial infections, and is especially prevalent in intensive care units, where both ... In healthy individuals, Acinetobacter colonies on the skin correlate with low incidence of allergies; Acinetobacter is thought ... Gene-silencing antisense oligomers in a form called peptide-conjugated phosphorodiamidate morpholino oligomers have also been ...
https://en.wikipedia.org/wiki/Acinetobacter
Bacteriological water analysisBacteriological water analysis
The unit of measurement is cfu/ml (or colony forming units per millilitre) and relates to the original sample. Calculation of ... samples contain a variety of components that can interfere with the ATP assay. The plate count method relies on bacteria ... Colonies that develop in the body of the medium can be counted by eye after incubation. The total number of colonies is ... growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate ...
https://en.wikipedia.org/wiki/Bacteriological_water_analysis
United States dollarUnited States dollar
... the Continental Congress and the Coinage Act prescribed a decimal system of units to go with the unit dollar, as follows: the ... These Pillars of Hercules on the silver Spanish dollar coins take the form of two vertical bars (,,) and a swinging cloth band ... Alexander Hamilton arrived at these numbers based on a treasury assay of the average fine silver content of a selection of worn ... the thirteen colonies became independent. Freed from British monetary regulations, they each issued £sd paper money to pay for ...
https://en.wikipedia.org/wiki/United_States_dollar
H2BK5acH2BK5ac
The complexes formed by the looping of the DNA are known as chromatin. The basic structural unit of chromatin is the nucleosome ... 3. Assay for transposase accessible chromatin sequencing (ATAC-seq) is used to look in to regions that are nucleosome free ( ... H2BK5ac elevated cell viability while the number of colonies and migration are impeded. The histone mark acetylation can be ... They are formed during the first stage of pregnancy and are the first cells to differentiate from the fertilized egg. or, after ...
https://en.wikipedia.org/wiki/H2BK5ac
Stem cellStem cell
Siminovitch L, McCulloch EA, Till JE (December 1963). "The distribution of colony-forming cells among spleen colonies". Journal ... "Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method". ... "Neurons derived from radial glial cells establish radial units in neocortex". Nature. 409 (6821): 714-720. Bibcode:2001Natur. ... In that same year, Siminovitch was a lead investigator for studies that found colony-forming cells were capable of self-renewal ...
https://en.wikipedia.org/wiki/Stem_cell
American Silver EagleAmerican Silver Eagle
This time his legislation took the form of an amendment (S.AMDT.418) to H.R. 47, the "Statue of Liberty-Ellis Island ... Secretary of the Treasury James A. Baker III presided over the striking ceremony held at the San Francisco Assay Office. ... The sets sold out at the issue limit of 75,000 units. In fewer than twenty known sets, the Sacagawea Dollars do not have a ... above the eagle are thirteen five-pointed stars representing the Thirteen Colonies. The reverse was inscribed with the phrases ...
https://en.wikipedia.org/wiki/American_Silver_Eagle
Hematopoietic stem cellHematopoietic stem cell
Colony-forming unit-megakaryocyte (CFU-Meg) Colony-forming unit-basophil (CFU-B) Colony-forming unit-eosinophil (CFU-Eos) The ... A cobblestone area-forming cell (CAFC) assay is a cell culture-based empirical assay. When plated onto a confluent culture of ... Colony-forming unit-lymphocyte (CFU-L) Colony-forming unit-erythrocyte (CFU-E) Colony-forming unit-granulocyte-macrophage (CFU- ... which is a cell counting unit.) There are various kinds of HSC colony-forming units: Colony-forming unit-granulocyte- ...
https://en.wikipedia.org/wiki/Hematopoietic_stem_cell
DNA microarrayDNA microarray
A form of replicate known as a dye flip can be performed to control for dye artifacts in two-channel experiments; for a dye ... Second, technical replicates (e.g. two RNA samples obtained from each experimental unit) may help to quantitate precision. The ... Microarray data is difficult to exchange due to the lack of standardization in platform fabrication, assay protocols, and ... and ant colony optimization. Input data for class prediction are usually based on filtered lists of genes which are predictive ...
https://en.wikipedia.org/wiki/DNA_microarray
Bacteriophage T12Bacteriophage T12
Titers of plaques can be found by diluting the samples and counting plaque-forming units (PFUs). Biochemical tests such as ... Plaque assays consist of pouring a soft agar solution with an indicator strain onto an agar plate. The indicator strain should ... In addition, none of the colonies containing the T12 genome was negative for speA, and therefore, the conclusion was drawn that ... "Plaque Assay Protocols". Microbe Library. American Society for Microbiology. Archived from the original on 30 November 2012. ...
https://en.wikipedia.org/wiki/Bacteriophage_T12
Beta-galactosidaseBeta-galactosidase
Each unit of β-galactosidase consists of five domains; domain 1 is a jelly-roll type β-barrel, domain 2 and 4 are fibronectin ... The β-galactosidase assay is used frequently in genetics, molecular biology, and other life sciences. An active enzyme may be ... The active ebg enzyme is an aggregate of ebgA -gene and ebgC-gene products in a 1:1 ratio with the active form of ebg enzymes ... After a time, certain colonies began to grow. However, the EbgA protein is an ineffective lactase and does not allow growth on ...
https://en.wikipedia.org/wiki/Beta-galactosidase
Microbiological cultureMicrobiological culture
Colony-forming unit Blood culture Microbial dark matter Microbial Food Cultures Screening cultures Sputum culture Synchronous ... Liquid cultures are ideal for preparation of an antimicrobial assay in which the experimenter inoculates liquid broth with ... Upon incubation, colonies will arise and single cells will have been isolated from the biomass. Once a microorganism has been ... Upon incubation, colonies will arise and single cells will have been isolated from the biomass. Once a microorganism has been ...
https://en.wikipedia.org/wiki/Microbiological_culture
AssayAssay
Depending on the nature of the Detection system assays can be based on: Colony forming or virtual colony count: e.g. by ... number of assays done per unit time (usually expressed as per hour) etc. Organizations or laboratories that perform Assays for ... DNase footprinting assay Filter binding assay Gel shift assay Bicinchoninic acid assay (BCA assay) Bradford protein assay Lowry ... Chemotaxis assay Secretion assays Apoptosis assays such as the DNA laddering assay, the Nicoletti assay, caspase activity ...
https://en.wikipedia.org/wiki/Assay
MelioidosisMelioidosis
On the agar plates, B. pseudomallei forms creamy, non-haemolytic, colonies after 2 days of incubation. After 4 days of ... Serological tests such as indirect haemagglutination assay (IHA) have been used to detect the presence of antibodies against B ... is the preferred antibiotic therapy for neurological melioidosis and those with septic shock admitted into intensive care units ... Colonies of B. pseudomallei that are grown on Francis medium (a modification of Ashdown medium with gentamicin concentration ...
https://en.wikipedia.org/wiki/Melioidosis
2 base encoding2 base encoding
Quality filtering of the reads can deliver higher raw accuracy reads which when aligned to form color contigs can deliver ... Therefore, since each color represents two nucleotides in which the second base of each dinucleotide unit constitutes the first ... a bead or a planar solid surface Amplification of DNA fragments on the solid surface using PCR and making polymerase colonies ... the use of emulsion PCR to generate millions of clonally amplified beads which one could perform these repeated ligation assays ...
https://en.wikipedia.org/wiki/2_base_encoding
Anaerobic digestionAnaerobic digestion
Many microorganisms affect anaerobic digestion, including acetic acid-forming bacteria (acetogens) and methane-forming archaea ... are fed into a crusher unit, where it is mixed with a small amount of water. The mixture is then fed into the bio-digester, ... The first known anaerobic digester was built in 1859 at a leper colony in Bombay in India. In 1895, the technology was ... Direct measurements of biomethanation potential are also made using gas evolution or more recent gravimetric assays. Using ...
https://en.wikipedia.org/wiki/Anaerobic_digestion
Coins of the pound sterlingCoins of the pound sterling
Assaying is now done by the Worshipful Company of Goldsmiths on behalf of HM Treasury. The 1p and 2p coins from 1971 are the ... All current coins carry a Latin inscription whose full form is ELIZABETH II DEI GRATIA REGINA FIDEI DEFENSATRIX, meaning " ... Chalmers, Robert (1893). A History of Currency in the British Colonies. London, UK: Her Majesty's Stationery Office. p. 110. ... which dropped the pound upon decimalisation by introducing dollars or new units worth 10 shillings or 1⁄2 pound). The following ...
https://en.wikipedia.org/wiki/Coins_of_the_pound_sterling
Rottnest IslandRottnest Island
A colony of Australian sea lions reside at Dyer Island and a colony of New Zealand fur seals (Arctophoca forsteri) reside at ... A sandy, low-lying island formed on a base of aeolianite limestone, Rottnest is an A-class reserve, the highest level of ... Rottnest Island Authority accommodation options include 291 villas, units and cottages which sleep 4, 6 or 8 people and which ... provisionally dated by aspartic acid racemisation assay of land snails to greater than 50 ka". Australian Archaeology. 49 (49 ...
https://en.wikipedia.org/wiki/Rottnest_Island
Glossary of numismaticsGlossary of numismatics
Each of these units is valued at one one-hundredth of its corresponding base unit, such as the dollar, euro, peso, etc. ... assay A test to ascertain the weight and purity of a coin. attribution An identifier of a coin, such as date, mint, ... ingot A bar of pure metal formed by pouring the molten metal into a mould. It may be stamped with its weight and purity. ... Spanish dollar A coin issued in Spain and its colonies from 1497 to 1864, equal to eight reales. It was legal tender in the ...
https://en.wikipedia.org/wiki/Glossary_of_numismatics
History of BostonHistory of Boston
It now forms the bulk of Boston Common, the largest public park in present-day downtown Boston. After Johnson's death the ... There were 1,502 units in the development on 50 acres (200,000 m2) of land. In 1966, the Columbia Point Health Center opened ... By 1625 the colony at Weymouth had failed and all of his fellow travelers returned to England. Blaxton remained, moving five ... Town officials in colonial Boston were chosen annually; positions included selectman, assay master, culler of staves, fence ...
https://en.wikipedia.org/wiki/History_of_Boston
The Wealth of NationsThe Wealth of Nations
The colony settled its own form of government, enacted its own laws, elected its own magistrates, and made peace or war with ... leading to a higher nominal output of units produced per time unit. Agriculture is less amenable than manufacturing to division ... Before coinage, people had to weigh and assay with each exchange, or risk "the grossest frauds and impositions." Thus nations ... Of Colonies: Of the Motives for establishing new Colonies: "The interest which occasioned the first settlement of the different ...
https://en.wikipedia.org/wiki/The_Wealth_of_Nations
Protein engineeringProtein engineering
A first round of PCR using these primer sets is performed and two double stranded DNA duplexes are formed. A second round of ... Plasmids are then isolated for colonies which show improved traits, and are then used as templates the next round of ... This results in the reconstruction of complete proteins by assembly of modules encoding different structural units.[page needed ... Also highly specific low throughput screening assays are increasingly applied in place of monumental screening and selection ...
https://en.wikipedia.org/wiki/Protein_engineering
SmallpoxSmallpox
In 1966 an international team, the Smallpox Eradication Unit, was formed under the leadership of an American, Donald Henderson ... As early as 1803, the Spanish Crown organized the Balmis expedition to transport the vaccine to the Spanish colonies in the ... Serologic tests and enzyme linked immunosorbent assays (ELISA), which measured variola virus-specific immunoglobulin and ... There are two forms of the smallpox, variola major is the severe and most common form, with a more extensive rash and higher ...
https://en.wikipedia.org/wiki/Smallpox
BoksburgBoksburg
Killian advised Dr W.E. Bok, Secretary of State for the Transvaal Republic, of the results of the assay. The result was the ... The club presently form part of the Falcons Rugby Union. Hoërskool Voortrekker (29 January 1920) - the first Afrikaans medium ... In addition to the K90 Centre, he has controlled the successful development of the motor dealership complex (a 115-unit ... Farrar hoped to become Prime Minister of the Transvaal Colony. In February 1907 elections were held for the Transvaal ...
https://en.wikipedia.org/wiki/Boksburg
Pathogenic Escherichia coliPathogenic Escherichia coli
However, current latex assays and some typing antisera have shown cross reactions with non-E. coli O157 colonies. Furthermore, ... Increased concern about the prevalence of this form of "superbug" in the United Kingdom has led to calls for further monitoring ... 1-40 units) Core region of phosphorylated nonrepeating oligosaccharides Lipid A (endotoxin) The O antigen is used for ... Growth on EMB agar produces black colonies with a greenish-black metallic sheen. This is diagnostic of E. coli. The organism is ...
https://en.wikipedia.org/wiki/Pathogenic_Escherichia_coli
Glycogen storage disease type IGlycogen storage disease type I
Granulocyte colony-stimulating factor (G-CSF), available as filgrastim, can reduce the risk of infection. In some cases, G-CSF ... The catalytic unit is associated with a calcium binding protein, and three transport proteins (T1, T2, T3) that facilitate ... Glycogen in liver and (to a lesser degree) kidneys serves as a form of stored, rapidly accessible glucose, so that the blood ... The diagnosis is definitively confirmed by liver biopsy with electron microscopy and assay of glucose-6-phosphatase activity in ...
https://en.wikipedia.org/wiki/Glycogen_storage_disease_type_I
Cameron County, TexasCameron County, Texas
FAA Airport Form 5010 for PIL PDF. Federal Aviation Administration, Effective 26 April 2018. Perez-Treviño, Emma. "Judge: Hard ... "DeWitt Colony Militia Captains". Tamu.edu. Archived from the original on January 10, 2011. Retrieved May 14, 2012. "2010 Census ... The population density was 370 people per square mile (143/km2). The 141,924 housing units averaged 132 per square mile (51/km2 ... and Implementation of Real-Time PCR Assays to Rapidly Genotype Resistance Mutations". Annals of the Entomological Society of ...
https://en.wikipedia.org/wiki/Cameron_County,_Texas
SerotoninSerotonin
Pheidole dentata takes on more and more tasks in the colony as it gets older, which requires it to respond to more and more ... The descending projections form a pathway that inhibits pain called the "descending inhibitory pathway" that may be relevant to ... because a binary logistic regression model used in these studies for the behavioral assay focused mostly on only one behavioral ... "Psychiatric Emergencies in the Intensive Care Unit". AACN Advanced Critical Care. 26 (4): 285-293, quiz 294-295. doi:10.4037/ ...
https://en.wikipedia.org/wiki/Serotonin
AnthraxAnthrax
The inhalation form presents with fever, chest pain and shortness of breath. The intestinal form presents with diarrhea (which ... B. anthracis colonies are medium-large, gray, flat, and irregular with swirling projections, often referred to as having a " ... Anthrax was first tested as a biological warfare agent by Unit 731 of the Japanese Kwantung Army in Manchuria during the 1930s ... To confirm the organism is B. anthracis, rapid diagnostic techniques such as polymerase chain reaction-based assays and ...
https://en.wikipedia.org/wiki/Anthrax
Mycobacterium tuberculosisMycobacterium tuberculosis
... while effective against childhood and severe forms of tuberculosis, has limited success in preventing the most common form of ... Visible colonies require several weeks to grow on agar plates. It is distinguished from other mycobacteria by its production of ... Assay Drug Dev Technol 14, 345-54, doi:10.1089/adt.2016.717 (2016). Takekawa, M., Maeda, T. & Saito, H. Protein phosphatase ... The third generation (mycobacterial interspersed repetitive unit - 2) added a further nine loci to bring the total to 24. This ...
https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis
Zinc finger chimeraZinc finger chimera
1013 colony-forming units of phage. Following incubation, the phage are removed and the plate washed with buffer containing 0.5 ... These assays are repeated using different target oligonucleotides. When investigating zinc fingers binding 5'-XNN-3' sequences ... After elution, the phage can be plated and DNA extracted from individual plaque forming units, restricted and the appropriately ... This forms the basis of the investigation of ZFPs binding by phage display. Work is typically performed using the murine ZFP-TF ...
https://en.wikipedia.org/wiki/Zinc_finger_chimera
Competition (biology)Competition (biology)
This form of competition typically manifests in new equilibrium abundances of each prey species. For example, suppose there are ... The higher the density of plants, the more plants will be present per unit ground area, and the stronger the competition will ... Barton, Kasey E.; Sanders, Nathan J.; Gordon, Deborah M. (2002-10-01). "The Effects of Proximity and Colony Age on ... in Laboratory Assays". Florida Entomologist. 102 (2): 322-328. doi:10.1653/024.102.0206. ISSN 0015-4040. S2CID 196662034. Holt ...
https://en.wikipedia.org/wiki/Competition_(biology)
Induced stem cellsInduced stem cells
... to form iNSCs that self-renew in culture and after transplantation and can survive and integrate without forming tumors in ... iPSCs have been shown to support the development of all-iPSC mice using a tetraploid (4n) embryo, the most stringent assay for ... Functional platelets are generated throughout the culture allowing the prospective collection of several transfusion units from ... the formation of keratinizing colonies from single cells". Cell. 6 (3): 331-43. doi:10.1016/S0092-8674(75)80001-8. PMID 1052771 ...
https://en.wikipedia.org/wiki/Induced_stem_cells
Frozen Cord Blood Proficiency Testing Program | STEMCELL TechnologiesFrozen Cord Blood Proficiency Testing Program | STEMCELL Technologies
Participants will be assessed on their proficiency at performing cord blood colony assays, focusing on sample preparation, ... For the hematopoietic colony forming unit (CFU) assay. Human Frozen Cord Blood Proficiency Testing Program - 1st Participant, ... The Proficiency Testing program is designed to standardize the hematopoietic colony-forming unit (CFU) assay. Participants will ... 2. Plating and Set-up of Colony CFU Assay. 3. Colony Enumeration and Identification. Proficiency Testing programs are available ...
https://www.stemcell.com/product-portfolios/cord-blood-banking/cell-processing/frozen-cord-blood-proficiency-testing-program.html
Methanol HPLC Plus, = 99.9 , poly-coated bottles 67-56-1Methanol HPLC Plus, = 99.9 , poly-coated bottles 67-56-1
Colony forming unit-fibroblast assay of bone marrow mononuclear cells. *As solvent for the preparation of extracts of hyphae of ...
https://www.sigmaaldrich.com/MX/en/product/sial/650609
Table 3 - Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast - Volume...
CSF, cerebrospinal fluid; CRAG, cryptococcal antigen; LFA, lateral flow immunochromatographic assay; CFU, colony-forming units ... Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast David R. Boulware ... Characteristics of CSF specimens with false-negative results by cryptococcal antigen lateral flow immunochromatographic assay, ... Multisite Validation of Cryptococcal Antigen Lateral Flow Assay and Quantification by Laser Thermal Contrast. ...
https://wwwnc.cdc.gov/eid/article/20/1/13-0906-t3.htm
Development of a Human Umbilical Cord-derived Mesenchymal Stromal Cells-based Advanced Therapy Medicinal Product to treat...Development of a Human Umbilical Cord-derived Mesenchymal Stromal Cells-based Advanced Therapy Medicinal Product to treat...
... clonogenicity were assessed using the Colony-Forming-Unit-Fibroblastic (CFU-F) assay. ... Potency assays To assess the immunomodulatory properties of UC-MSCs, we performed, as potency assays, the following two assays ... MLR potency assay was performed according to Nicotra et al. (16). MLR assay was performed on UC-MSCs both in a resting state ( ... e. Potency assay. MLR potency assay was performed as described above. T cells proliferation was calculated using the area under ...
https://www.researchsquare.com/article/rs-754265/v1
Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus | Arthritis...Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus | Arthritis...
Expression of apoptosis inhibiting caspase 8L was analyzed in PBMNCs and CPCs by gene transcript and protein expression assays ... nor were differences observed in the potential to form clusters on Matrigel. The expression of caspase 8L was reduced at the ... migratory capacity and the potential to form clusters on Matrigel were determined. ... The endothelial-like phenotype was confirmed and the colony forming unit (CFU) capacity, ...
https://arthritis-research.biomedcentral.com/articles/10.1186/ar2283
Mechanisms of Antileukemic Activity of the Novel Bcl-2 Homology Domain-3 Mimetic GX15-070 (Obatoclax) | Cancer Research |...Mechanisms of Antileukemic Activity of the Novel Bcl-2 Homology Domain-3 Mimetic GX15-070 (Obatoclax) | Cancer Research |...
AML blast colony and colony-forming unit-granulocyte-macrophage assays. AML bone marrow cells were isolated by gradient ... A colony was defined as a cluster of 40 or more cells [blasts (colony-forming unit, CFU) or erythrocyte (blast-forming unit), ... A. Samples used for colony assays 1 BM 47 RAEBT 46,XX,del(5)(q13q33)[2],42-46,XX,-2,-3,del(4)(q25q31.2),del(5)(q13q33),-6,-7, ... A. Samples used for colony assays 1 BM 47 RAEBT 46,XX,del(5)(q13q33)[2],42-46,XX,-2,-3,del(4)(q25q31.2),del(5)(q13q33),-6,-7, ...
https://aacrjournals.org/cancerres/article/68/9/3413/543142/Mechanisms-of-Antileukemic-Activity-of-the-Novel
Pesquisa | Portal Regional da BVSPesquisa | Portal Regional da BVS
Colony-forming unit assay was implemented for evaluating intracellular growth of the MTB strains. Enzyme-linked immunosorbent ... In contrast to arterial-like, sinusoidal-like BMECs are short-lived, form 2D-networks, contribute to in vivo angiogenesis, and ... assay was used for measurement of inflammatory cytokines. Real-time quantitative polymerase chain reaction was utilized to ...
https://pesquisa.bvsalud.org/portal/?lang=pt&q=au:%22Chen,%20Zhen%22
Inhibitory effect of baicalin on bacillary loads. Bacterial CFUs were... | Download Scientific DiagramInhibitory effect of baicalin on bacillary loads. Bacterial CFUs were... | Download Scientific Diagram
... we performed the colony forming unit (CFU) counting assay. As shown in Figure 3, FIGURE 7 , Bacalin suppresses the PI3K/Akt/ ... In this review, we will explore the implications of autophagy and its selective forms in several lung infections, such as SARS- ...
https://www.researchgate.net/figure/Inhibitory-effect-of-baicalin-on-bacillary-loads-Bacterial-CFUs-were-counted-after_fig3_320801231
NIOSHTIC-2 Search Results - Full View
Assays of burst forming unit erythroid and colony forming unit erythroid (CFUE) cells were determined using a modified plasma ... Granulocyte macrophage colony forming cells were assayed using lung media. Litter sizes, weights, and number of dead or ... Mice exposed in utero to low concentrations of benzene exhibit enduring changes in their colony forming hematopoietic cells. ... Exposure to 20ppm caused increases in the numbers of granulocytic macrophage colony forming cells among 2 day old neonates. ...
http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=benzene&f1=%2A&Adv=0&terms=1&PageNo=40&RecNo=400&View=f&
Vaccines | Free Full-Text | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental...Vaccines | Free Full-Text | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental...
The presence of viable Aspergillus in lungs was quantified by colony-forming unit (CFU) counting assay (H). Fungal DNA levels ... The presence of viable Aspergillus in lungs was quantified by colony-forming unit (CFU) counting assay (H). Fungal DNA levels ... 1010 colony-forming units (CFU)/mL of PBS. For oral administration of bacteria, 7-day-old turkeys (n = 20) received E. coli ... The qPCR assays were run under the following conditions: 50 °C for 2 min, 95 °C for 10 min, then 45 cycles of 15 s at 95 °C and ...
https://www.mdpi.com/2076-393X/8/2/285
Comparative investigations on the protective effects of rhodioside, ciwujianoside-B and astragaloside IV on radiation injuries...Comparative investigations on the protective effects of rhodioside, ciwujianoside-B and astragaloside IV on radiation injuries...
Peripheral blood leucocytes and endogenous spleen colony counts, colony-forming unit-granulocyte macrophage assay, analysis of ... spleen colonies and granulocyte-macrophage colonies in mice exposed to 6.0 Gy γ-radiation. Rhodioside showed more protective ... The fragmentation of double-stranded DNA in lymphocytes was detected by the comet assay. The changes in cell cycle were ... were measured by western blot and the electrophoretic mobility shift assay. The results showed that pretreatment with all of ...
https://pubmed.ncbi.nlm.nih.gov/21031634/
Therapeutic inhibition of FcγRIIb signaling targets leukemic stem cells in chronic myeloid leukemia | LeukemiaTherapeutic inhibition of FcγRIIb signaling targets leukemic stem cells in chronic myeloid leukemia | Leukemia
Colony-forming unit (CFU) assays using Bcr-Abl+ or ev lin− FcγRIIb+/+ and FcγRIIb−/− cells revealed significantly decreased ... Numbers of colonies (colony-forming units, CFUs) were assessed after 7 days. Re-plating was performed using 1 × 104 cells. CFU ... and colony-forming units were counted after 7-9 days.. Immunoblotting. Immunoblotting was performed as described previously [23 ... 4c). Leukemic FcγRIIb−/− cells tended to form smaller and more diffuse colonies, compared to leukemic FcγRIIb+/+ cells. This ...
https://www.nature.com/articles/s41375-020-0977-8?error=cookies_not_supported
US Patent for Deadman and passcode microbial kill switches Patent (Patent # 11,459,573 issued October 4, 2022) - Justia...US Patent for Deadman and passcode microbial kill switches Patent (Patent # 11,459,573 issued October 4, 2022) - Justia...
Survival assays. Colony forming unit (CFU) cell viability assays were used to measure functionality of the Deadman and Passcode ... Upon removal of ATc, the toxins produced 3-5 logs of killing within 6 hours as measured by colony forming units (CFUs) (. FIG. ... Upon removal of ATc, the toxins produced 3-5 logs of killing within 6 hours as measured by colony forming units (CFUs) (. FIG. ... In these assays, the TetR inhibitor anhydrotetracycline (ATc; 100 ng/mL) was included in the media to ensure TF expression from ...
https://patents.justia.com/patent/11459573
TSAP Thermosensitive Alkaline Phosphatase Activity in NEB Restriction Enzyme BuffersTSAP Thermosensitive Alkaline Phosphatase Activity in NEB Restriction Enzyme Buffers
TSAP activity was assessed using both a functional cloning assay and gel electrophoresis analysis. We found TSAP active in all ... To account for this uncut background in the functional cloning assay, the colony forming units (cfu) from the ligated samples ... TSAP activity was assayed by counting the number of colony forming units (cfu) obtained from plasmid that had been digested, ... TSAP activity was also tested in a functional cloning assay. Fifty nanograms of the unligated and ligated samples from the ...
https://www.promega.com/resources/pubhub/enotes/tsap-thermosensitive-alkaline-phosphatase-activity-in-neb-buffers/
Apitherapy News: October 2010Apitherapy News: October 2010
... of colony-forming units were carried out and the endotoxin was quantified by the chromogenic Limulus amobocyte lysate assay. ... Since 2006, 20 to 40 percent of the bee colonies in the United States alone have suffered "colony collapse." Suspected culprits ... unit. The amino acid sequence of the tryptic glycopeptide harboring the T-antigen unit was G-E-S-L-X-K (X might be glycosylated ... The resin -- called propolis once it gets to the colony -- helps the bees immune systems, theyve discovered.. But there are " ...
https://apitherapy.blogspot.com/2010/10/
Rab5a GTPase regulates fusion between pathogen-containing phagosomes and cytoplasmic organelles in human neutrophils | Journal...Rab5a GTPase regulates fusion between pathogen-containing phagosomes and cytoplasmic organelles in human neutrophils | Journal...
... bacteria was assessed at each step by comparing bacterial counts determined by microscopy and assays of colony forming units ( ... An overlay assay utilizing 1 μCi/ml of [α-32P] GTP (3.000 Ci/mM, DuPont NEN) in 50 mM phosphate buffer (pH 7.5) containing 5 mM ... In our assay, an insufficient amount of MCP was recovered after less than 15 minutes of phagocytosis, hence the kinetic ... The GTP-binding state of Rab5a was detected on MCPv, MCPa and SCP by an [α-32P] GTP overlay assay and visualized by ...
https://journals.biologists.com/jcs/article/115/6/1321/2835/Rab5a-GTPase-regulates-fusion-between-pathogen
Team:Caltech/Project/Oxidative Burst - 2008.igem.orgTeam:Caltech/Project/Oxidative Burst - 2008.igem.org
Survival of the target strain was assayed by a colony forming unit (CFU) count both immediately upon introduction and after 6 ... Coculture Assay. Table 1 - CFU counts of a competing E. coli strain before and after co-culturing with the engineered strain. ... This "oxidative burst" is sufficient to kill a competing strain of JI377 cells in co-culture assays. A genetic circuit was also ... Hydrogen peroxide in the supernatant was assayed every half hour. The arrow indicates induction with AHL at time 0 hr. Error ...
https://2008.igem.org/wiki/index.php?title=Team:Caltech/Project/Oxidative_Burst&diff=prev&oldid=57614
MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011MESH TREE NUMBER CHANGES - 2012 MeSH. August 19, 2011
E5.240.374 Colony Count, Microbial E1.370.225.875.220 Colony-Forming Units Assay E1.370.225.500.383 E5.242.383 Color G1.201. ... A-Form G2.111.570.790.486.128 G5.360.580.114 DNA, C-Form G2.111.570.790.486.156 G5.360.580.128 DNA, Catenated G2.111.570.790. ... E5.240.550 Plaque Assay E1.370.225.875.970.790 (Replaced for 2012 by Viral Plaque Assay) Plastic Embedding E1.370.225.500. ... Z-Form G2.111.570.790.486.493 G5.360.580.493 Donor Selection E4.932.500 E4.936.537.500 Dose-Response Relationship, Radiation ...
https://decs.bvs.br/P/mnchg2012.txt
SDF-1 Effect on Hematopoietic Progenitor and Stem Cell Mobilization: R&D SystemsSDF-1 Effect on Hematopoietic Progenitor and Stem Cell Mobilization: R&D Systems
... and peripheral blood in colony-forming assays and scoring the colony forming units (CFUs). AdSDF1-induced migration of ... significant numbers of colony-forming units-spleen (CFU-S). Both AdNull and AdMIP3 alpha controls were found to be ineffective ...
https://www.rndsystems.com/resources/articles/sdf-1-effect-hematopoietic-progenitor-and-stem-cell-mobilization
Compared to the amniotic membrane, Whartons jelly may be a more suitable source of mesenchymal stem cells for cardiovascular...Compared to the amniotic membrane, Wharton's jelly may be a more suitable source of mesenchymal stem cells for cardiovascular...
Colony-forming unit fibroblast (CFU-F) assay. The self-renewal capacity of WJ-MSCs and AM-MSCs was evaluated using the CFU-F ... Both types of cells displayed colony-forming ability, and high colony-forming efficiencies were observed in WJ-MSCs compared to ... CFU-F colony-forming unit fibroblast, WJ-MSCs Whartons jelly mesenchymal stem cells ... d Proliferation and metabolism of WJ-MSCs and AM-MSCs as investigated by the MTT assay. (c and d) Mean ± SD of five independent ...
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-017-0501-x
Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing - Fingerprint - Oregon...Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing - Fingerprint - Oregon...
Colony-Forming Units Assay 100% * Electric Impedance 69% * Bone Marrow Cells 32% ... Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing. ... Dive into the research topics of Rapid assay of stem cell functionality and potency using electric cell-substrate impedance ...
https://ohsu.pure.elsevier.com/en/publications/rapid-assay-of-stem-cell-functionality-and-potency-using-electric-2/fingerprints/
JCI Insight - Bone marrow Tregs mediate stromal cell function and support hematopoiesis via IL-10JCI Insight - Bone marrow Tregs mediate stromal cell function and support hematopoiesis via IL-10
... we used the colony-forming unit (CFU) assay to test if IL-10 influenced the clonogenic potential of stromal progenitors. Stroma ... Thymocyte transplant assay. FAC-sorted CD3+ DN (CD4-CD8-Foxp3-) thymocytes from Foxp3+(CD45.1) mice were transplanted retro- ... Treg homing assay. FAC-sorted Foxp3+ cells were isolated from BM or spleen of CD45.2+ mice were transplanted retro-orbitally ... In our assay, HSC numbers indicate stem cell survival and act as a surrogate measure of stromal cell function (HSC support). ...
https://insight.jci.org/articles/view/135681
Aldosterone Receptors - KRAS-G12C RESEARCHAldosterone Receptors - KRAS-G12C RESEARCH
Colony forming unit (CFU) assay is definitely widely used as an signal to anticipate the achievement of engraftment, since ... immediate quantitative assay for HSC proliferation is normally unavailable. The purpose of this …. Read More ...
http://mingsheng88.org/category/aldosterone-receptors/
Gundeep Dhillon, MD, MPHs Profile | Stanford ProfilesGundeep Dhillon, MD, MPH's Profile | Stanford Profiles
... and granulocyte/macrophage colony-forming unit (CFU-GM) assays of the bone marrow progenitor cells.Administration of ethanol ... Hematopoiesis was examined by erythroid colony forming unit (CFU-E) ... Confirmation of infection was made by testing serum and CSF for both WNV RNA by RT-PCR and WNV IgM by serological assays. ... CMV-Specific T-Cell Responses in Lung Transplant Patients Using a Monocyte-Derived Dendritic Cell (MDDC) Assay. Chen, S. F., ...
https://profiles.stanford.edu/gundeep-dhillon
Búsqueda | BVS CLAP/SMR-OPS/OMSBúsqueda | BVS CLAP/SMR-OPS/OMS
Severe fetal anemia caused by anti-Jra : Burst forming unit-erythroid colony formation inhibition assay suggesting possible ...
https://pesquisa.bvsalud.org/perinatal/?lang=es&q=au:Clarke,+Gwen
Nitric oxide-releasing microparticles as a potent antimicrobial therapeutic against chronic rhinosinusitis bacterial isolates<...Nitric oxide-releasing microparticles as a potent antimicrobial therapeutic against chronic rhinosinusitis bacterial isolates<...
The susceptibility of bacterial isolates from CRS patients to SNO-MP was evaluated through a colony forming unit (CFU) assay. ... The susceptibility of bacterial isolates from CRS patients to SNO-MP was evaluated through a colony forming unit (CFU) assay. ... The susceptibility of bacterial isolates from CRS patients to SNO-MP was evaluated through a colony forming unit (CFU) assay. ... The susceptibility of bacterial isolates from CRS patients to SNO-MP was evaluated through a colony forming unit (CFU) assay. ...
https://einstein.pure.elsevier.com/en/publications/nitric-oxide-releasing-microparticles-as-a-potent-antimicrobial-t
Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy<...
... were compared by colony-forming unit assay, BrdU assay, flow cytometry, and β-galactosidase activity assay, respectively. Their ... were compared by colony-forming unit assay, BrdU assay, flow cytometry, and β-galactosidase activity assay, respectively. Their ... were compared by colony-forming unit assay, BrdU assay, flow cytometry, and β-galactosidase activity assay, respectively. Their ... were compared by colony-forming unit assay, BrdU assay, flow cytometry, and β-galactosidase activity assay, respectively. Their ...
https://mayoclinic.pure.elsevier.com/en/publications/altered-fate-of-tendon-derived-stem-cells-isolated-from-a-failed-
IMSEAR at SEARO: Study of hematopoietic progenitor cells in thalassemia after bone marrow transplantation.
Colony-Forming Units Assay. en_US. dc.subject.mesh. Cyclophosphamide --therapeutic use. en_US. ...
https://imsear.searo.who.int/handle/123456789/31766?mode=full
DeCSDeCS
Colony Forming Units Assay Colony Forming Units Assays Colony-Forming Units Assays Stem Cell Assay Stem Cell Assays ... Colony Forming Units Assay. Colony Forming Units Assays. Colony-Forming Units Assays. Stem Cell Assay. Stem Cell Assays. ... Assay, Clonogenic Cell Assay, Colony-Forming Units Assay, Stem Cell Assays, Clonogenic Cell Assays, Colony-Forming Units Assays ... Assay, Clonogenic Cell. Assay, Colony-Forming Units. Assay, Stem Cell. Assays, Clonogenic Cell. Assays, Colony-Forming Units. ...
https://decs.bvsalud.org/en/ths/resource/?id=3145
Code System Concept
Colony forming unit-granulocyte assay (procedure). Code System Preferred Concept Name. Colony forming unit-granulocyte assay ( ... Colony forming unit-granulocyte assay Active Synonym false false 75405010 CFU-G assay Active Synonym false false ...
https://phinvads.cdc.gov/vads/ViewCodeSystemConcept.action?oid=2.16.840.1.113883.6.96&code=45223006
Unique fingerprintBone marrowInhibitionDilution assayPolymerase Chain RePeripheralMacrophageHematopoietic cellsBiofilmsCFUsSpleenMICROBIALGranulocyteSusceptibilityAntigenVirulenceApoptosisVivoFetalIncubationBurstIsolatesCellsSecretionQuantitativeMolecularBacterialCellStrainsMarkersRapidFlowClustersDeterminationResultsTimeAnalysisCOUNTSamplesTechniquePositiveTestGiveWaterPotentialPatients
Unique fingerprint1
Bone marrow1
  • Colony forming unit-fibroblast assay of bone marrow mononuclear cells. (sigmaaldrich.com)
Inhibition2
  • Burst forming unit-erythroid colony formation inhibition assay suggesting possible erythroid suppression mechanism. (bvsalud.org)
  • This activity has been evaluated in terms of microbial growth and cellular doubling time (optical density, colony forming units and doubling time assays), inhibition/persistence (crystal violet staining), viability of microbial cells embedded in the biofilms (live/dead stain) and pyoverdine production (fluorimetric assay). (unimore.it)
Dilution assay2
  • The agar plate dilution method was used to count the CFU of viability of S. mutans KCTC 3065 that was determined by enumeration (colony forming units) in an agar dilution assay using no OFI (control), or 5, 20, and 50 mg/mL OFI following incubation for 0, 6, 12, and 24 h. (papersearch.net)
  • Cancer stem cells (CSC) targeting was studied by mRNA and protein expression of CSC markers, by limiting dilution assay, and by flow cytometric and immunofluorescent evaluation of CSC mitochondrial and cellular oxidative stress. (biomedcentral.com)
Polymerase Chain Re3
  • Finally, a Polymerase Chain Reaction (PCR) assay to determine the most prevalent species of Enterococcus spp. (bvsalud.org)
  • Although the specific cDNA amplification mechanisms of reverse-transcriptase polymerase chain reaction (RT-PCR) and RT loop-mediated isothermal amplification (RT-LAMP) are very different, both molecular assays serve as options to detect arboviral RNA in mosquito pools. (cdc.gov)
  • Detection of viral genomic sequences in autopsy tissue, serum, or cerebral spinal fluid (CSF) samples via reverse-transcriptase polymerase chain reaction (RT-PCR) assay: RT-PCR provides earlier and more specific diagnosis. (medscape.com)
Peripheral4
  • Peripheral blood leucocytes and endogenous spleen colony counts, colony-forming unit-granulocyte macrophage assay, analysis of DNA content and apoptosis rate determination were performed to evaluate the effects of the three glycosides on hematogenesis. (nih.gov)
  • The increased number of HPCs were monitored by seeding cells collected from BM, spleen, and peripheral blood in colony-forming assays and scoring the colony forming units (CFUs). (rndsystems.com)
  • Critical limb ischemia (CLI) is the most severe form of atherosclerotic peripheral arterial disease (PAD). (biomedcentral.com)
  • Treatment of the Brtl mice with the proteasome inhibitor Bortezomib ameliorated both osteoblast differentiation in vitro and bone properties in vivo as demonstrated by colony-forming unit-osteoblasts assay and peripheral quantitative computed tomography analysis on long bones, respectively. (elsevier.com)
Macrophage4
  • Granulocyte macrophage colony forming cells were assayed using lung media. (cdc.gov)
  • Exposure to 20ppm caused increases in the numbers of granulocytic macrophage colony forming cells among 2 day old neonates. (cdc.gov)
  • The results showed that pretreatment with all of the glycosides improved survival time and increased the number of leucocytes, spleen colonies and granulocyte-macrophage colonies in mice exposed to 6.0 Gy γ-radiation. (nih.gov)
  • [ 10 , 11 ] Orf also produces protein that inhibits IL-2 and granulocyte macrophage-colony stimulating factor and a vascular endothelial growth factor homolog. (medscape.com)
Hematopoietic cells1
  • Mice exposed in utero to low concentrations of benzene exhibit enduring changes in their colony forming hematopoietic cells. (cdc.gov)
Biofilms2
  • The aim of this in vitro study was to evaluate the efficacy of Cupral® on planktonic forms of some pathogens, as well as to assess its ability to prevent and affect the formation/persistence of microbial biofilms. (unimore.it)
  • The ability to form biofilms is a common feature of microorganisms, such as bacteria or fungi. (mdpi.com)
CFUs1
  • 100,000 colony forming units (CFUs) of a bacteria to be considered an infection, whereas this number is less in a catheterized patient. (medscape.com)
Spleen2
  • AdSDF1-induced migration of significant numbers of colony-forming units-spleen (CFU-S). Both AdNull and AdMIP3 alpha controls were found to be ineffective in mobilizing significant numbers of HPCs and HSCs. (rndsystems.com)
  • Although TLR ligands induced in situ migration of spleen cDC into the T cell area, spleen pDCs formed clusters in the marginal zone and in the outer T cell area 6 h after injection of TLR9 and TLR7 ligands, respectively. (rupress.org)
MICROBIAL1
  • To characterize these isolates, a funnel approach, involving numerous phenotypic and genomic screening assays, was applied to identify kefir-derived microbial strains with the highest probiotic potential. (frontiersin.org)
Granulocyte1
  • Granulocyte-monocyte colony-forming unit assays demonstrated colonies from the products cryopreserved using passive freezing (both laboratories), but no colonies from products cryopreserved using the CRF. (core.ac.uk)
Susceptibility3
  • The susceptibility of bacterial isolates from CRS patients to SNO-MP was evaluated through a colony forming unit (CFU) assay. (elsevier.com)
  • Etest Assay Etest assay has been recently used to evaluate susceptibility to antibiotics in Bartonella . (medscape.com)
  • Antibiotic susceptibility testing using the Etest assay for Bartonella henselae with rifampin Estrip showing minimum inhibitory concentration value. (medscape.com)
Antigen1
  • We report the results of a laboratory sensitivity and specificity evaluation of the Rapid Analyte Measurement Platform (RAMP) Dengue Virus (DENV) antigen detection assay, which is designed to detect all serotypes of DENV in mosquito pools. (cdc.gov)
Virulence3
  • The intended uses for this candidate BCG sub-strain specific Reference Reagent is as a comparator for consistency monitoring in viability assays, including cultural viable count and modified ATP assays, residual virulence, local reactogenicity and protection assays in animal models. (who.int)
  • In this study we demonstrated the efficacy of the Arabidopsis seedling assay to study 1) the virulence factors of P. syringae pv. (biomedcentral.com)
  • This assay could also provide an excellent system for investigating the virulence mechanisms of P. syringae . (biomedcentral.com)
Apoptosis2
  • Expression of apoptosis inhibiting caspase 8L was analyzed in PBMNCs and CPCs by gene transcript and protein expression assays. (biomedcentral.com)
  • Protection factors were determined for these thiols against radiation-induced apoptosis (Apoalert assay), mitotic cell death (clonogenic assay) and double-strand break (dsb) induction (pulse field gel electrophoresis) in MR4 and 3.7 cells. (ox.ac.uk)
Vivo2
  • S-MSCs have the ability to form tube-like structures and, in vivo, to induce blood flow recovery. (biomedcentral.com)
  • For the in vivo assay, rabbits (n = 120) with meniscal lesions were divided into 3 groups: repair with high-purity magnesium stitches (Mg group), repair with absorbable sutures (Control group), and no repair (Blank group). (ctimeetingtech.com)
Fetal2
  • Effects of low level maternal inhalation exposure to benzene (1076433) (Bz) on erythrocytic and granulocytic colony forming cells of fetal, neonatal, and young adult offspring were studied. (cdc.gov)
  • Moreover, CD7+ cells isolated from fetal liver contained all cells in this tissue capable of forming CFU-T colonies in vitro. (duke.edu)
Incubation1
  • This method has several advantages over conventional soil-grown plant inoculation assays, including a shorter growth and incubation period, ease of inoculation and handling, uniform infection and disease development, requires less growth chamber space and is suitable for high-throughput screens. (biomedcentral.com)
Burst1
  • Assays of burst forming unit erythroid and colony forming unit erythroid (CFUE) cells were determined using a modified plasma clot method. (cdc.gov)
Isolates3
  • Well diffusion assays were conducted to determine the propensity of kefir isolates to inhibit growth of enteric pathogens, and a competitive adhesion/exclusion assay was used to determine the ability of kefir isolates to out-compete or exclude attachment of enteric pathogens to Caco-2 cells. (frontiersin.org)
  • This study in a tertiary care hospital in Egypt used real-time PCR assay to test ertapenem-nonsusceptible isolates of K. pneumoniae for the presence of the blaKPC gene and compared the results with modified Hodge test. (who.int)
  • Cefoxitin (30g) was used as surrogate to determine phenotypic methicillin resistance in staphylococcus isolates, and the methicillin resistance ( mec A) gene was detected by conventional PCR assay. (who.int)
Cells3
  • A cytologic technique for measuring the functional capacity of stem cells by assaying their activity. (bvsalud.org)
  • Cells were characterized by the expression of cell surface markers, the relative expression of 6 genes, the secretion of 10 cytokines and the ability to form vessel-like structures. (biomedcentral.com)
  • DENV vaccine based on this approach was prepared for production on an industrial scale in France using specific-pathogen-free (SPF) dog colony and FRhL cells. (who.int)
Secretion1
  • If additional agars are used, it will grow pink on MacConkey's agar, and may have a darker color around the colony due to secretion of the bile salts. (medscape.com)
Quantitative2
  • Colony forming unit (CFU) assay is definitely widely used as an signal to anticipate the achievement of engraftment, since immediate quantitative assay for HSC proliferation is normally unavailable. (mingsheng88.org)
  • Washout of Nitrospira and Nitrosomonas from the trickling biofilter reactors was also examined by quantitative PCR assay. (hokudai.ac.jp)
Molecular2
  • Michael Andreeff, Section of Molecular Hematology and Therapy, Department of Stem Cell Transplantation and Cellular Therapy, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Unit 448, Houston, TX 77030. (aacrjournals.org)
  • In addition, it may be as a Reference BCG sub-strain in identity assays using molecular biology techniques, such as mPCR. (who.int)
Bacterial3
  • In addition they support other applications like the IFUSPOT Assay (Inclusion Forming Units), Plaque Assays or Bacterial Colony Counting. (temaricerca.com)
  • Seven bacterial strains of Lentilactobacillus hilgardii , Lacticaseibacillus paracasei , Liquorilactobacillus satsumensis , Lactobacillus helveticus , and Lentilactobacillus kefiri , were ultimately identified as potential probiotics, and combined to form a "kefir probiotics blend. (frontiersin.org)
  • If the fungal colonies are not well separated from bacterial colonies or there is insufficient material for testing, subculture the organism (see procedures 8.4 and 8.7). (singletonapps.com)
Cell1
  • Conversely, mutant MSCs generated more colony-forming unit-adipocytes compared to WT, with more adipocytes per colony, and increased number and size of triglyceride drops per cell. (elsevier.com)
Strains1
  • The RAMP DENV assay was able to detect geographically distinct strains of all 4 DENV serotypes in virus-spiked mosquito pools that contained at least 4.3 log10 plaque forming units/ml, although discrete sensitivity limits varied slightly for each serotype. (cdc.gov)
Markers1
  • Their osteogenic and chondrogenic differentiation potentials and mRNA expression of tendon-related markers were compared using standard assays. (elsevier.com)
Rapid1
  • The Arabidopsis seedling flood-inoculation assay provides a rapid, efficient and economical method for studying Arabidopsis-Pseudomonas interactions with minimal growth chamber space and time. (biomedcentral.com)
Flow1
  • Outcome parameters will be monitored and quantified by flow cytometry, histochemistry, confocal microscopy, scanning electron microscopy, and colony forming unit assays. (unthsc.edu)
Clusters2
  • The endothelial-like phenotype was confirmed and the colony forming unit (CFU) capacity, migratory capacity and the potential to form clusters on Matrigel were determined. (biomedcentral.com)
  • Migration on vascular endothelial growth factor showed no significant differences, nor were differences observed in the potential to form clusters on Matrigel. (biomedcentral.com)
Determination1
  • Spectrophotometric methods and enzyme-linked immunosorbent assay were used for parameters' determination. (torlakinstitut.com)
Results2
  • Pro-Lab Diagnostics, Inc., Round Rock, Texas) and compared the results to a validated real-time RT-PCR assay. (cdc.gov)
  • Laboratory generated virus stock samples containing 2.3 log10 plaque-forming units (PFU)/ml and intrathoracically inoculated mosquitoes containing 2.4 log10 PFU/ml produced positive results in the Pro-AmpRT WNV assay. (cdc.gov)
Time2
  • colony forming unit (cfu) assay and time until positive culture in the BACTEC radiometric liquid system between 14 HIV-infected and 22 non-HIV-Infected adults with initial episodes of smear-positive polmunary TB at baseline and during treatment with standard four-drug SCC. (bvsalud.org)
  • Of field-collected pools that were WNV positive by real-time RT-PCR, 74.5% (70 of 94) were also positive by the Pro-AmpRT WNV assay, resulting in an overall Cohen's kappa agreement of 79.4% between the 2 tests. (cdc.gov)
Analysis1
  • k q r2 distance Unit Analysis r newton · metre2 coulomb2 · coulomb metre2 newton coulomb N·m2 · C N C2 m2 C Note: This equation applies only to the field surrounding an isolated point charge. (qrforex.com)
COUNT1
  • The lyophilised ampoule preparation was quantified using cultural viable count and modified ATP assay and its identity was confirmed using multiplex PCR (mPCR). (who.int)
Samples1
  • The total and respirable dust samples were assayed for endotoxins. (cdc.gov)
Technique1
  • the concentration corresponds to approximately 106 colony-forming unit/ml, as determined using the colony-forming unit technique. (medscape.com)
Positive1
  • 1 BoNTs are produced In Taiwan (China), two outbreaks have been recorded, by Clostridium botulinum , a Gram-positive, rod- one caused by type A botulism in nine patients who con- shaped, anaerobic, spore-forming, motile bacterium. (who.int)
Test1
  • La présente étude menée dans un hôpital de soins tertiaires en Égypte a utilisé la méthode de PCR en temps réel pour évaluer la présence du gène blaKPC dans les isolats de K. pneumoniae non sensibles à l'ertapénème, puis a comparé les résultats à l'aide du test de Hodge modifié. (who.int)
Give2
  • Window air-conditioning units also give lower indoor pollen levels than those found in naturally ventilated buildings. (com.ng)
  • 225. Document Bibliographic Information: Location In Book: CURRENT DIAGNOSIS TREATMENT IN INFECTIOUS DISEASES (2001) Section I. Scallable the number is corrected to give colony forming units per mL (remember, you only put 100 μL onto the Petri dish). (qrforex.com)
Water1
  • The vaccine was presented in a lyophilized (freeze-dried) form and reconstituted with water for injection in order to deliver a 0.5 ml specified dose. (who.int)
Potential1
  • An initial investigation was conducted to confirm the outbreak and to form hypotheses about the potential causes, followed by a case-control assessment of the plausible causative food item. (who.int)
Patients1
  • RÉSUMÉ L'émergence et la propagation rapide des souches de Klebsiella pneumoniae résistantes aux antibiotiques et porteuses du gène blaKPC codant la production de carbapénèmases ont compliqué la prise en charge des infections des patients. (who.int)
Frozen Cord Blood Proficiency Testing Program | STEMCELL Technologies
Frozen Cord Blood Proficiency Testing Program | STEMCELL Technologies (stemcell.com)
Development of a Human Umbilical Cord-derived Mesenchymal Stromal Cells-based Advanced Therapy Medicinal Product to treat...
Development of a Human Umbilical Cord-derived Mesenchymal Stromal Cells-based Advanced Therapy Medicinal Product to treat... (researchsquare.com)
Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus | Arthritis...
Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus | Arthritis... (arthritis-research.biomedcentral.com)
Mechanisms of Antileukemic Activity of the Novel Bcl-2 Homology Domain-3 Mimetic GX15-070 (Obatoclax) | Cancer Research |...
Mechanisms of Antileukemic Activity of the Novel Bcl-2 Homology Domain-3 Mimetic GX15-070 (Obatoclax) | Cancer Research |... (aacrjournals.org)
Vaccines | Free Full-Text | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental...
Vaccines | Free Full-Text | Gut Microbiota Abrogates Anti-α-Gal IgA Response in Lungs and Protects against Experimental... (mdpi.com)
TSAP Thermosensitive Alkaline Phosphatase Activity in NEB Restriction Enzyme Buffers
TSAP Thermosensitive Alkaline Phosphatase Activity in NEB Restriction Enzyme Buffers (promega.com)
Apitherapy News: October 2010
Apitherapy News: October 2010 (apitherapy.blogspot.com)
JCI Insight - Bone marrow Tregs mediate stromal cell function and support hematopoiesis via IL-10
JCI Insight - Bone marrow Tregs mediate stromal cell function and support hematopoiesis via IL-10 (insight.jci.org)
Gundeep Dhillon, MD, MPH's Profile | Stanford Profiles
Gundeep Dhillon, MD, MPH's Profile | Stanford Profiles (profiles.stanford.edu)
Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing - Fingerprint - Oregon...
Rapid assay of stem cell functionality and potency using electric cell-substrate impedance sensing - Fingerprint - Oregon... (ohsu.pure.elsevier.com)
Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy<...
Altered fate of tendon-derived stem cells isolated from a failed tendon-healing animal model of tendinopathy<... (mayoclinic.pure.elsevier.com)
In vivo efficacy of endothelial growth medium stimulated mesenchymal stem cells derived from patients with critical limb...
In vivo efficacy of endothelial growth medium stimulated mesenchymal stem cells derived from patients with critical limb... (translational-medicine.biomedcentral.com)
Stem Cells Archives | Regenexx® Pittsburgh
Stem Cells Archives | Regenexx® Pittsburgh (regenexxpittsburgh.com)
Cancer Stem Cells-Perspectives on Current Status and Future Directions: AACR Workshop on Cancer Stem Cells | Cancer Research |...
Cancer Stem Cells-Perspectives on Current Status and Future Directions: AACR Workshop on Cancer Stem Cells | Cancer Research |... (aacrjournals.org)
NVS
NVS (vocab.nerc.ac.uk)
Smartphone-Based Genetic Testing Identifies Hospital Pathogens
Smartphone-Based Genetic Testing Identifies Hospital Pathogens (medscape.com)