A cytologic technique for measuring the functional capacity of stem cells by assaying their activity.
Hospital units providing continuous surveillance and care to acutely ill patients.
A subtype of non-receptor protein tyrosine phosphatases that contain two SRC HOMOLOGY DOMAINS. Mutations in the gene for protein tyrosine phosphatase, non-receptor type 11 are associated with NOONAN SYNDROME.
A genetically heterogeneous, multifaceted disorder characterized by short stature, webbed neck, ptosis, skeletal malformations, hypertelorism, hormonal imbalance, CRYPTORCHIDISM, multiple cardiac abnormalities (most commonly including PULMONARY VALVE STENOSIS), and some degree of INTELLECTUAL DISABILITY. The phenotype bears similarities to that of TURNER SYNDROME that occurs only in females and has its basis in a 45, X karyotype abnormality. Noonan syndrome occurs in both males and females with a normal karyotype (46,XX and 46,XY). Mutations in a several genes (PTPN11, KRAS, SOS1, NF1 and RAF1) have been associated the the NS phenotype. Mutations in PTPN11 are the most common. LEOPARD SYNDROME, a disorder that has clinical features overlapping those of Noonan Syndrome, is also due to mutations in PTPN11. In addition, there is overlap with the syndrome called neurofibromatosis-Noonan syndrome due to mutations in NF1.
An enzyme group that specifically dephosphorylates phosphotyrosyl residues in selected proteins. Together with PROTEIN-TYROSINE KINASE, it regulates tyrosine phosphorylation and dephosphorylation in cellular signal transduction and may play a role in cell growth control and carcinogenesis.
New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors.
A Src-homology domain-containing protein tyrosine phosphatase found in the CYTOSOL of hematopoietic cells. It plays a role in signal transduction by dephosphorylating signaling proteins that are activated or inactivated by PROTEIN-TYROSINE KINASES.
An autosomal dominant disorder with an acronym of its seven features (LENTIGO; ELECTROCARDIOGRAM abnormalities; ocular HYPERTELORISM; PULMONARY STENOSIS; abnormal genitalia; retardation of growth; and DEAFNESS or SENSORINEURAL HEARING LOSS). This syndrome is caused by mutations of PTPN11 gene encoding the non-receptor PROTEIN TYROSINE PHOSPHATASE, type 11, and is an allelic to NOONAN SYNDROME. Features of LEOPARD syndrome overlap with those of NEUROFIBROMATOSIS 1 which is caused by mutations in the NEUROFIBROMATOSIS 1 GENES.
Form of adoptive transfer where cells with antitumor activity are transferred to the tumor-bearing host in order to mediate tumor regression. The lymphoid cells commonly used are lymphokine-activated killer (LAK) cells and tumor-infiltrating lymphocytes (TIL). This is usually considered a form of passive immunotherapy. (From DeVita, et al., Cancer, 1993, pp.305-7, 314)
Manipulation of the host's immune system in treatment of disease. It includes both active and passive immunization as well as immunosuppressive therapy to prevent graft rejection.
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
A former branch of knowledge embracing the study, description, and classification of natural objects (as animals, plants, and minerals) and thus including the modern sciences of zoology, botany, and mineralogy insofar as they existed at that time. In the 17th, 18th, and 19th centuries it was much used for the generalized pursuit of certain areas of science. (Webster, 3d ed; from Dr. James H. Cassedy, NLM History of Medicine Division)
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
Transfer of HEMATOPOIETIC STEM CELLS from BONE MARROW or BLOOD between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS). Hematopoietic stem cell transplantation has been used as an alternative to BONE MARROW TRANSPLANTATION in the treatment of a variety of neoplasms.
A clinical syndrome characterized by repeated spontaneous hemorrhages and a remarkable increase in the number of circulating platelets.
A particular zone of tissue composed of a specialized microenvironment where stem cells are retained in a undifferentiated, self-renewable state.
A myeloproliferative disorder of unknown etiology, characterized by abnormal proliferation of all hematopoietic bone marrow elements and an absolute increase in red cell mass and total blood volume, associated frequently with splenomegaly, leukocytosis, and thrombocythemia. Hematopoiesis is also reactive in extramedullary sites (liver and spleen). In time myelofibrosis occurs.
A Janus kinase subtype that is involved in signaling from GROWTH HORMONE RECEPTORS; PROLACTIN RECEPTORS; and a variety of CYTOKINE RECEPTORS such as ERYTHROPOIETIN RECEPTORS and INTERLEUKIN RECEPTORS. Dysregulation of Janus kinase 2 due to GENETIC TRANSLOCATIONS have been associated with a variety of MYELOPROLIFERATIVE DISORDERS.
Progenitor cells from which all blood cells derive.
Conditions which cause proliferation of hemopoietically active tissue or of tissue which has embryonic hemopoietic potential. They all involve dysregulation of multipotent MYELOID PROGENITOR CELLS, most often caused by a mutation in the JAK2 PROTEIN TYROSINE KINASE.
A mechanism of communication within a system in that the input signal generates an output response which returns to influence the continued activity or productivity of that system.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A family of intracellular CYSTEINE ENDOPEPTIDASES that play a role in regulating INFLAMMATION and APOPTOSIS. They specifically cleave peptides at a CYSTEINE amino acid that follows an ASPARTIC ACID residue. Caspases are activated by proteolytic cleavage of a precursor form to yield large and small subunits that form the enzyme. Since the cleavage site within precursors matches the specificity of caspases, sequential activation of precursors by activated caspases can occur.
A short pro-domain caspase that plays an effector role in APOPTOSIS. It is activated by INITIATOR CASPASES such as CASPASE 9. Isoforms of this protein exist due to multiple alternative splicing of its MESSENGER RNA.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
A group of pathogens comprising the smallest known agents of infectious disease. They are unencapsulated and are capable of replicating autonomously in susceptible cells. Positively identified viroids composed of single-stranded RNA have been isolated from higher plants, but the existence of DNA viroids pathogenic to animals is suspected.
A long pro-domain caspase that contains a caspase recruitment domain in its pro-domain region. Caspase 9 is activated during cell stress by mitochondria-derived proapoptotic factors and by CARD SIGNALING ADAPTOR PROTEINS such as APOPTOTIC PROTEASE-ACTIVATING FACTOR 1. It activates APOPTOSIS by cleaving and activating EFFECTOR CASPASES.
A severe sometimes chronic anemia, usually macrocytic in type, that does not respond to ordinary antianemic therapy.
The production of red blood cells (ERYTHROCYTES). In humans, erythrocytes are produced by the YOLK SAC in the first trimester; by the liver in the second trimester; by the BONE MARROW in the third trimester and after birth. In normal individuals, the erythrocyte count in the peripheral blood remains relatively constant implying a balance between the rate of erythrocyte production and rate of destruction.
The cells in the erythroid series derived from MYELOID PROGENITOR CELLS or from the bi-potential MEGAKARYOCYTE-ERYTHROID PROGENITOR CELLS which eventually give rise to mature RED BLOOD CELLS. The erythroid progenitor cells develop in two phases: erythroid burst-forming units (BFU-E) followed by erythroid colony-forming units (CFU-E); BFU-E differentiate into CFU-E on stimulation by ERYTHROPOIETIN, and then further differentiate into ERYTHROBLASTS when stimulated by other factors.
Anemia characterized by the presence of erythroblasts containing excessive deposits of iron in the marrow.
Chronic refractory anemia with granulocytopenia, and/or thrombocytopenia. Myeloblasts and progranulocytes constitute 5 to 40 percent of the nucleated marrow cells.
Glycoprotein hormone, secreted chiefly by the KIDNEY in the adult and the LIVER in the FETUS, that acts on erythroid stem cells of the BONE MARROW to stimulate proliferation and differentiation.
Acquired defect of cellular immunity that occurs in mice infected with mouse leukemia viruses (MuLV). The syndrome shows striking similarities with human AIDS and is characterized by lymphadenopathy, profound immunosuppression, enhanced susceptibility to opportunistic infections, and B-cell lymphomas.
Virus diseases caused by the RETROVIRIDAE.
Species of GAMMARETROVIRUS, containing many well-defined strains, producing leukemia in mice. Disease is commonly induced by injecting filtrates of propagable tumors into newborn mice.
Family of RNA viruses that infects birds and mammals and encodes the enzyme reverse transcriptase. The family contains seven genera: DELTARETROVIRUS; LENTIVIRUS; RETROVIRUSES TYPE B, MAMMALIAN; ALPHARETROVIRUS; GAMMARETROVIRUS; RETROVIRUSES TYPE D; and SPUMAVIRUS. A key feature of retrovirus biology is the synthesis of a DNA copy of the genome which is integrated into cellular DNA. After integration it is sometimes not expressed but maintained in a latent state (PROVIRUSES).
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
The soft tissue filling the cavities of bones. Bone marrow exists in two types, yellow and red. Yellow marrow is found in the large cavities of large bones and consists mostly of fat cells and a few primitive blood cells. Red marrow is a hematopoietic tissue and is the site of production of erythrocytes and granular leukocytes. Bone marrow is made up of a framework of connective tissue containing branching fibers with the frame being filled with marrow cells.
Transfer of MESENCHYMAL STEM CELLS between individuals within the same species (TRANSPLANTATION, HOMOLOGOUS) or transfer within the same individual (TRANSPLANTATION, AUTOLOGOUS).
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Euploid male germ cells of an early stage of SPERMATOGENESIS, derived from prespermatogonia. With the onset of puberty, spermatogonia at the basement membrane of the seminiferous tubule proliferate by mitotic then meiotic divisions and give rise to the haploid SPERMATOCYTES.
Cells with high proliferative and self renewal capacities derived from adults.

Increase of hematopoietic responses by triple or single helical conformer of an antitumor (1-->3)-beta-D-glucan preparation, Sonifilan, in cyclophosphamide-induced leukopenic mice. (1/3030)

It has been suggested that the immunopharmacological activity of soluble (1-->3)-beta-D-glucan depends on its conformation in mice. In this study, we examined the relationship between the conformation of Sonifilan (SPG) and hematopietic responses in cyclophosphamide (Cy)-induced leukopenic mice. SPG, a high molecular weight (1-->3)-beta-D-glucan, has a triple helical conformation in water, and it was changed by treatment with aqueous sodium hydroxide to the single helical conformer (SPG-OH). The effects of SPG or SPG-OH on hematopoietic responses in cyclophosphamide induced leukopenic mice were investigated by monitoring i) gene expression of cytokines by RT-PCR, ii) protein synthesis of interleukin 6 (IL-6) by ELISA and iii) colony formation of bone marrow cells (BMC). The mice administered Cy and SPG or SPG-OH expressed and produced higher levels of IL-6 mRNA and protein than the mice administered only Cy. Gene expression of NK1.1 was also induced by Cy/SPG (or SPG-OH) treatment. Induced gene expression of stem cell factor (SCF) and macrophage-colony stimulating factor (M-CSF) by SPG/SPG-OH were also found in in vitro culture of BMC from Cy treated mice. These results strongly suggested that conformation of the glucans, single and triple helix, are independent of the hematopietic response.  (+info)

Functional characterization of a novel hematopoietic stem cell and its place in the c-Kit maturation pathway in bone marrow cell development. (2/3030)

While the majority of purified pluripotential hematopoietic stem cells (PHSC) express c-Kit, the receptor for steel factor, we have phenotypically and functionally separated a distinct class of PHSC that does not express c-Kit. In contrast to c-Kit-positive (c-Kit(pos)) PHSC, the c-Kit-negative (c-Kit(neg)) PHSC do not proliferate in response to multiple hematopoietic growth factors in vitro and do not radioprotect or form macroscopic spleen colonies (CFU-s) when transplanted into lethally irradiated recipients. However, the c-Kit(neg) PHSC show delayed or slow reconstitution kinetics when cotransplanted with radioprotective bone marrow cells. c-Kit(neg) PHSCs cells can give rise to c-Kit(pos) cells with CFU-s activity, radioprotective activity, and PHSC activity. Thus, constitutive hematopoiesis is maintained by c-Kit(pos) PHSCS cells that are recruited from a more primitive quiescent c-Kit(neg) PHSC population, which represents a critical developmental stage in definitive hematopoiesis.  (+info)

Single leukapheresis products collected from healthy donors after the administration of granulocyte colony-stimulating factor contain ten-fold higher numbers of long-term reconstituting hematopoietic progenitor cells than conventional bone marrow allografts. (3/3030)

Cytokine-mobilized peripheral blood progenitor cells (PBPCs) have been used successfully for hematopoietic reconstitution following allogeneic transplantation. The ease of harvest, the faster engraftment and the high yield of CD34+ cells have made this source of hematopoietic progenitor cells (HPCs) an attractive alternative to bone marrow (BM). In the present study we compared the engraftment potential of conventional BM allografts and single leukapheresis products (LPs) collected from healthy donors following the administration of granulocyte colony-stimulating factor (G-CSF). For this, lineage-committed and primitive HPCs were assessed by flow cytometry and by colony- and cobblestone area-forming cell (CFC, CAFC) assays. Mean numbers of CD34+ cells in LPs (n = 11) were similar to that of BM grafts (n = 12) (278+/-57 vs 227+/-34 x 10(6) CD34+ cells). The frequencies of CFCs, week 5 CAFCs and week 8 CAFCs were 1.6-, 8.4- and 10.3-fold higher in the CD34+ compartment of mobilized blood than that of marrow, resulting in significantly higher yields of clonogenic HPCs in LPs when compared to BM grafts. We conclude that G-CSF preferentially mobilizes clonogenic progenitors capable of short- and, in particular, longterm reconstitution, and that the engraftment potential of single LPs is superior to that of BM allografts. Hence, the use of PBPCs may be favorable for protocols that include graft manipulations with expected cell loss (eg T cell depletion, CD34+ selection). PBPCs may also be advantageous for gene therapy trials due to their high numbers of potential target cells (eg CAFCs).  (+info)

Influence of increased c-Myc expression on the growth characteristics of human melanoma. (4/3030)

Overexpression of the proto-oncogene c-myc has been associated with neoplastic transformation in a variety of tumors. For human melanoma high c-myc expression has been found in the vertical growth phase and higher positivity reported in metastases than primary tumors. The principle aim of this study was to determine, whether c-Myc expression influences the metastatic behavior of human melanoma in the absence of lymphocyte-mediated immune phenomena. The growth characteristics and tumor biology of two c-myc transfectants of the human melanoma cell line IGR39D, expressing c-Myc 1.7 and three times over baseline and the respective vector control were analyzed both in vitro and in a severe combined immunodeficient mouse model in vivo. Both c-myc transfectants showed increased growth rates, anchorage independent growth and directed cell movement in culture. Subcutaneously implanted IGR39D melanomas highly overexpressing c-Myc spontaneously formed macroscopic metastases (lymph nodes and lung) in severe combined immunodeficient mice in all cases (n = 7 per group), whereas less prominent c-Myc overexpression caused the development of only lung micrometastases. During the time period leading to terminal disease in animals injected with c-myc transfected human melanoma cells, melanoma development was not seen in vector controls. These findings suggest that constitutive high c-Myc expression in human melanoma results in a more aggressive growth behavior both in vitro and in vivo and favors metastasis in severe combined immunodeficient mice by factors unrelated to immune phenomena such as class I human leukocyte antigen downregulation known to be associated with c-Myc expression.  (+info)

Differential behaviors toward ultraviolet A and B radiation of fibroblasts and keratinocytes from normal and DNA-repair-deficient patients. (5/3030)

Xeroderma pigmentosum (XP) and trichothiodystrophy (TTD) are rare genodermatoses transmitted as recessive and autosomal traits that result in reduced capacity to repair UV-induced DNA lesions. Although XP, but not TTD, patients are prone to basal and squamous cell carcinomas, to date no comparative studies of the XP and TTD phenotypes have included epidermal keratinocytes. We compared the DNA repair capacity (by unscheduled DNA synthesis) and cell survival (by clonal analysis) of epidermal keratinocytes and dermal fibroblasts grown from normal individuals and patients with xeroderma pigmentosum and trichothiodystrophy following UVA and UVB irradiation. The same dose of UVB (1000 J/m2) induced twice as many DNA lesions in normal fibroblasts as in normal keratinocytes. UV survival rates were always higher in keratinocytes than in fibroblasts. Normal and TTD keratinocytes survived better following UVA and UVB irradiation than XP-C and XP-D keratinocytes. XP-C keratinocytes exhibited exacerbated sensitivity toward UVA radiation. Unscheduled DNA synthesis at UV doses leading to 50% cell survival indicated that the ratio of DNA repair capacity to cell survival is higher in keratinocytes than in fibroblasts. In addition, UVA and UVB irradiation induced a transition from proliferative to abortive keratinocyte colonies. This transition varied between donors and was in part correlated with their cancer susceptibility. Altogether these data provide the first evidence of the differential behaviors of normal, XP, and TTD keratinocytes toward UV radiation.  (+info)

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing. (6/3030)

Uncontrolled-rate freezing techniques represent an attractive alternative to controlled-rate cryopreservation procedures which are time-consuming and require high-level technical expertise. In this study, we report our experience using uncontrolled-rate cryopreservation and mechanical freezer storage at -140 degrees C. Twenty-eight PBPC samples (10 cryovials, 18 freezing bags) from 23 patients were cryopreserved in a cryoprotectant solution composed of phosphate-buffered saline (80%, v/v) supplemented with human serum albumin (10%, v/v) and dimethylsulfoxide (10%, v/v). The cryopreservation procedure required on average 1.5 h. The mean (+/- s.e.m.) storage time of cryovials and bags was 344+/-40 and 299+57 days, respectively. Although cell thawing was associated with a statistically significant reduction of the absolute number of nucleated cells (vials: 0.3x10(9) vs. 0.2x10(9), P< or =0.02; bags: 14x10(9) vs. 11x10(9), P< or =0.0003), the growth of committed progenitors was substantially unaffected by the freezing-thawing procedure, with mean recoveries of CFU-Mix, BFU-E, and CFU-GM ranging from 60+/- 29% to 134+/-15%. Mean recoveries of LTC-IC from cryovials and bags were 262+/-101% and 155+/-27% (P< or =0.2), respectively. In 14 out of 23 patients who underwent high-dose chemotherapy and PBPC reinfusion, the pre-and post-freezing absolute numbers of hematopoietic progenitors cryopreserved in bags were compared. A significant reduction was detected for CFU-Mix (11 vs. 7.4x10(5)), but no significant loss of BFU-E (180 vs. 150x10(5)), CFU-GM (400 vs. 290x10(5)) and LTC-IC (15 vs. 16x10(5)) could be demonstrated. When these patients were reinfused with uncontrolled-rate cryopreserved PBPC, the mean number of days to reach 1x10(9)/l white blood cells and 50x10(9)/l platelets were 9 and 13, respectively. In conclusion, the procedure described here is characterized by short execution time, allows a substantial recovery of primitive and committed progenitors and is associated with prompt hematopoietic recovery following myeloablative therapy even after long-term storage.  (+info)

Conclusions of a national multicenter intercomparative study of in vitro cultures of human hematopoietic progenitors. (7/3030)

With the aim of developing a standardized program of clonogenic cultures, a multicenter intercomparative study of human CFU-GM, BFU-E and CFU-GEMM cultures was conducted. Aliquots of fresh mononuclear cord blood cells, as well as uniform culture materials and instructions for cell culture and for colony scoring were distributed to 28 national laboratories involved in hematopoietic research and transplantation. High interlaboratory coefficients of variation (CV) in the reported number of progenitors were found in our first intercomparative study (range 67-231%). To investigate the relevance of colony scoring in variations of the reported colony numbers, participants were invited to attend a meeting where a single culture dish was scored. In this case, the CVs ranged from 31% to 81%. A subsequent intercomparative assay was then conducted, and significant reductions in the inter-laboratory CVs were obtained with respect to the first study (CVs for colonies grown with two different media: CFU-GMs, 48% and 55%; BFU-Es, 70% and 62%; CFU-GEMMs, 70% and 51%; respectively). In most instances CVs were not significantly different from those obtained in the single plate scoring study, suggesting that the scoring process was the most relevant parameter accounting for variations in the reported colony numbers.  (+info)

Role of interleukin 10 and transforming growth factor beta1 in the angiogenesis and metastasis of human prostate primary tumor lines from orthotopic implants in severe combined immunodeficiency mice. (8/3030)

Transfection of primary human prostate tumor cells (i.e., HPCA-10a, 10b, 10c, and 10d lines) with the transforming growth factor (TGF)-beta1 gene stimulated anchorage-independent growth and promoted tumor growth, angiogenesis, and metastasis after orthotopic implantation in severe combined immunodeficiency mice. In contrast, interleukin (IL)-10 transfected cells or cells cotransfected with these two genes exhibited reduced growth rates and significantly reduced angiogenesis and metastasis after 8, 12, and 16 weeks. Enzyme-linked immunosandwich assays confirmed that the respective tumors expressed elevated levels of TGF-beta1 and IL-10 in vivo. ELISAs further showed that TGF-beta1 expression induced matrix metalloproteinases-2 (MMP-2) expression, whereas IL-10 down-regulated MMP-2 expression while up regulating TIMP-1 in the transfected cells. Also, tumor factor VIII levels correlated with TGF-beta1 and MMP-2 expression and inversely with IL-10 and TIMP-1 levels. More importantly, mouse survival was zero after 4-6 months in mice bearing TGF-beta1- and MMP-2-expressing tumors and increased significantly in mice implanted with IL-10- and TIMP-1-expressing tumors (i.e., to >80% survival). Analysis of the metastatic lesions showed that they expressed TGF-beta1 and MMP-2 but barely detectable levels of IL-10 or TIMP-1, suggesting that IL-10 and TIMP-1 might normally block tumor growth, angiogenesis, and metastasis.  (+info)

CFU-GM - Colony-Forming Unit-Granulocyte and Monocyte. Looking for abbreviations of CFU-GM? It is Colony-Forming Unit-Granulocyte and Monocyte. Colony-Forming Unit-Granulocyte and Monocyte listed as CFU-GM
We studied the adhesion of primitive and committed progenitors from chronic myelogenous leukemia (CML) and normal bone marrow to stroma and to several extracellular matrix components. In contrast to benign primitive progenitors from CML or normal bone marrow, Ph1-positive primitive progenitors from CML bone marrow fail to adhere to normal stromal layers and to fibronectin and its proteolytic fragments, but do adhere to collagen type IV, an extracellular matrix component of basement membranes. Similarly, multilineage colony-forming unit (CFU-MIX) progenitors from CML bone marrow do not adhere to fibronectin or its adhesion promoting fragments but adhere to collagen type IV. Unlike committed progenitors from normal bone marrow, CML single-lineage burst-forming units-erythroid and granulocyte/macrophage colony-forming units fail to adhere to fibronectin or its components but do adhere to both collagen type IV and laminin. Evaluation of adhesion receptor expression demonstrates that fibronectin ...
Antiangiogenic therapy of cancer is highly attractive for several reasons (reviewed in Refs. 38, 39, 40 ). For instance, it can potentially overcome three major problems associated with other anticancer therapies, i.e., the problems of drug resistance (41 , 42) , poor delivery (43 , 44) , and tumor heterogeneity. One approach to the antiangiogenic therapy is antibody-based targeting of tumor vasculature. We have been targeting EDG on tumor vasculature in vivo (9 , 15 , 16) . Several features of EDG are described above (see Introduction). In addition, our anti-EDG mAbs showed little reactivity with normal human bone marrow cells (1) . This observation is consistent with our later finding that anti-EDG immunotoxin (i.e., ricin A-chain conjugate of SN6) selectively eradicated EDG-expressing cells without severely damaging normal human hematopoietic progenitors in the colony-forming unit assays.4 The results suggest that administration of therapeutic doses of an appropriate anti-EDG mAb and ...
Bone Marrow Cells;Cell Count;Cell Differentiation;Cells, Cultured;Colony-Forming Units Assay;Fetal Blood--cytology;Hematopoietic Stem Cells--cytology; ...
Cell Cycle--drug effects;Cells, Cultured;Colony-Forming Units Assay;Hematopoietic Stem Cells--drug effects;Interleukin-3;Fluorouracil;Lymphokines--pharmacology;Mice;Spleen-- ...
Definition of colony-forming units in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is colony-forming units? Meaning of colony-forming units as a legal term. What does colony-forming units mean in law?
We previously demonstrated that 3-azido-3-deoxythymidine (AZT) inhibits growth proliferation of human bone marrow progenitor cells in vitro [Antimicrob. Agents Chemother. 31:452-454 (1987)]. The present study evaluates the effect of toxic concentrations of AZT on possible sites of toxicity in human bone marrow cells. Exposure of cells over a 6-hr period to AZT concentrations between 0.5 and 50 microM resulted in a decreased incorporation of tritiated deoxyguanosine into DNA. Unchanged AZT and its phosphorylated metabolites accumulated within cells after exposure to 10 microM [3H]AZT. 3-Azido-3-deoxythymidine-5-monophosphate was the predominant metabolite, reaching a concentration of 49.2 +/- 14.1 pmol/10(6) cells after 48 hr, and a continuous increase was observed in all phosphorylated derivative levels between 2 and 48 hr of incubation. Using a highly sensitive and specific DNA polymerase assay, endogenous deoxyribonucleotide pool size(s) were analyzed for 48 hr after incubation of cells ...
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Myelotoxicity of pesticides and algal toxin was detected in vitro by using the granulocyte-macrophage colony forming unit assay (CFU-GM), and the MTT test with SR-4987 cells, an established stromal cell line derived from a long term murine bone marrow culture, which may represent a suitable in vitro model for studying haematotoxicity. Comparison of the IC50s and NOELs obtained with the CFU-GM assay and those determined by testing the established stromal cells in the MTT cytotoxicity test indicate that inhibition of the proliferation of SR-4987 stromal cells is a sensitive in vitro endpoint for measuring myelotoxicity. It is suggested that this assay could be used as rapid and easy sceening test for determining the haematoxicity of environmental toxins. A comparison with results obtained with the MTT test on a non-differentiated cell line, 3T3-L1, was carried out to distinguish between non-specific interference with cell proliferation and specific toxicity on haemopoietic cells ...
Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted by.
TY - JOUR. T1 - Endothelial colony-forming cell role in neoangiogenesis and tissue repair. AU - Critser, Paul J.. AU - Yoder, Mervin C.. PY - 2010/2/1. Y1 - 2010/2/1. N2 - Purpose of review: Patients suffering from vascular disease often have impaired angiogenic ability contributing to impaired tissue repair. One potential therapy is to deliver cells that can aid in angiogenesis. This review will discuss the ability of endothelial progenitor cells (EPCs), which have been reported to contribute to neoangiogenesis in both physiological and pathological conditions, to contribute to neoangiogenesis in tissue repair. Recent findings: In recent years, various reports have described conflicting roles for EPC in vessel formation. Currently there are three different assays for outgrowth of EPC all resulting in the isolation of different cell populations. This confusion is partially due to limited functional characterization of putative EPC populations. One population, endothelial colony-forming cell ...
Laminins are alphabetagamma heterotrimeric extracellular proteins that regulate cellular functions by adhesion to integrin and nonintegrin receptors. Laminins containing alpha4 and alpha5 chains are expressed in bone marrow, but their interactions with hematopoietic progenitors are unknown. We studied human bone marrow cell adhesion to laminin-10/11 (alpha5beta1gamma1/alpha5beta2gamma1), laminin-8 (alpha4beta1gamma1), laminin-1 (alpha1beta1gamma1), and fibronectin. About 35% to 40% of CD34(+) and CD34(+)CD38(-) stem and progenitor cells adhered to laminin-10/11, and 45% to 50% adhered to fibronectin, whereas they adhered less to laminin-8 and laminin-1. Adhesion of CD34(+)CD38(-) cells to laminin-10/11 was maximal without integrin activation, whereas adhesion to other proteins was dependent on protein kinase C activation by 12-tetradecanoyl phorbol-13-acetate (TPA). Fluorescence-activated cell-sorting (FACS) analysis showed expression of integrin alpha6 chain on most CD34(+) and CD34(+)CD38(-) cells.
BACKGROUND: Endothelial progenitor cells play a pivotal role in tissue repair, and thus are used for cell replacement therapies in regenerative medicine. We tested whether the anesthetic sevoflurane would modulate growth or mobilization of these angiogenic cells. METHODS: In an in vitro model, mononuclear cells isolated from peripheral blood of healthy donors were preconditioned with sevoflurane (3 times 30 min at 2 vol% interspersed by 30 min of air). Colony-forming units were determined after 9 days in culture and compared with time-matched untreated control. Using magnetic cell sorting, CD133+/CD34+ endothelial progenitors were enriched from human umbilical cord blood, and vascular endothelial growth factor (VEGF), VEGFR2 (KDR), granulocyte colony-stimulating factor (G-CSF), STAT3, c-kit, and CXCR4 expressions were determined in sevoflurane-treated and untreated cells by real-time reverse transcriptase polymerase chain reaction. In a volunteer study with crossover design, we tested whether ...
Umbilical cord blood-derived endothelial colony-forming cells (UCB-ECFC) show utility in neovascularization, but their contribution to osteogenesis has not been defined. Cocultures of UCB-ECFC with human fetal-mesenchymal stem cells (hfMSC) resulted in earlier induction of alkaline phosphatase (ALP) (Day 7 vs. 10) and increased mineralization (1.9x; p < .001) compared to hfMSC monocultures. This effect was mediated through soluble factors in ECFC-conditioned media, leading to 1.8-2.2x higher ALP levels and a 1.4-1.5x increase in calcium deposition (p < .01) in a dose-dependent manner. Transcriptomic and protein array studies demonstrated high basal levels of osteogenic (BMPs and TGF-βs) and angiogenic (VEGF and angiopoietins) regulators. Comparison of defined UCB and adult peripheral blood ECFC showed higher osteogenic and angiogenic gene expression in UCB-ECFC. Subcutaneous implantation of UCB-ECFC with hfMSC in immunodeficient mice resulted in the formation of chimeric human vessels, ...
Programmed cell death-2 (PDCD2) protein is enriched in embryonic, hematopoietic, and neural stem cells, however, its function in stem/progenitor cell differentiation is unclear. We investigated the effects of PDCD2 knockdown on the development and differentiation of hematopoietic progenitor cells (HPC). CD34(+) cells derived from normal human bone marrow and K562 leukemic cells were effectively transduced with short-hairpin RNA to knockdown PDCD2. Colony-forming assays were used to investigate the effects of PDCD2 loss on HPC clonogenic potential and on 12-O-tetradecanoyl-phorbol-13-acetate-and arabinofuranosylcytosine-induced terminal differentiation. In CD34(+) clonogenic progenitors, PDCD2 knockdown decreased the total number of colony-forming units, increased the number of colony-forming units-granulocyte-erythroid-macrophage-megakaryocyte and burst-forming unit-erythroid primitive colonies, and decreased the number of burst-forming unit-erythroid mature colonies. Similar results were ...
To expand on the findings from previous clinical trials that the growth of residual tumor is increased at a predictable time following initial drug administration, malignant plasma cells from bone marrows of patients with multiple myeloma (MM) were examined for changes in proliferation and clonogenicity induced in vivo by cyclophosphamide and in vitro by drug-induced humoral stimulatory activity. Peak plasma cell [3H]thymidine labeling index (LI) occurred predictably following drug and paralleled changes in agar colony formation by marrow cells obtained during therapy. Colony-forming capacity of pretreatment MM marrow populations was enhanced when those cells were cultured with humoral stimulatory activity, similar to the increased colony formation detected in Day 9 postcyclophosphamide marrows at the time of peak plasma cell LI.. To further define a relationship between proliferative plasma cells and colony-forming tumor cells, MM Marrows were fractionated by sedimentation on an isokinetic ...
Lung Basal Stem Cells Rapidly Repair DNA Damage Using the Error-Prone Nonhomologous End-Joining Pathway Investigators showed that human basal stem cells (BSCs) isolated from heavy smokers proliferate extensively, whereas their alveolar progenitor cell counterparts have limited colony-forming capacity. They demonstrated that this difference arises in part because of the ability of BSCs to repair their DNA more efficiently than alveolar cells following ionizing radiation or chemical-induced DNA damage. [PLoS Biol] Full Article , Press Release Differentiated State of Initiating Tumor Cells Is Key to Distinctive Immune Responses Seen in H-RasG12V-Induced Squamous Tumors In two doxycycline-inducible models where oncogenic H-RasG12V is targeted either to the epidermal basal/stem cell layer with a Keratin14-rtTA transgene, or committed progenitor/suprabasal cells with an Involucrin-tTA transgene, investigators observed strikingly distinct tumor immune responses. These data showed that position of tumor ...
Effects of 1R-Chl on colony formation of CML patient MNCs and normal human MNCs.(A) MNCs from CML patients were cultured with rh stem cell factor, rh IL-3, rh G
Vascular Incorporation of Endothelial Colony-Forming Cells Is Essential for Functional Recovery of Murine Ischemic Tissue following Cell Therapy
SmartDish™ is meniscus-free cultureware that improves the accuracy of manual and automated counting of hematopoietic colony assays.
SmartDish™ is meniscus-free cultureware that improves the accuracy of manual and automated counting of hematopoietic colony assays.
SmartDish™ is meniscus-free cultureware that improves the accuracy of manual and automated counting of hematopoietic colony assays.
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We evaluated the effects of natural purine and pyrimidine nucleosides on protection from or reversal of 3-azido-3-deoxythymidine (AZT) cytotoxicity in human bone marrow progenitor cells by using clonogenic assays. The selectivity of the protection or rescue agents was examined in evaluating the antiretroviral activity of AZT in combination with these modulating agents and of AZT alone. Following exposure of human granulocyte-macrophage progenitor cells for 2 h to 5 microM AZT (70% inhibitory concentration), increasing concentrations of potential rescue agents were added. Cells were cultured, and colony formation was assessed after 14 days. At concentrations of up to 50 microM no natural 2-deoxynucleosides, including thymidine, were able to reverse the toxic effects of AZT. Dose-dependent reversal was observed with uridine and cytidine, and essentially complete reversal was achieved with 50 microM uridine. In the protection studies, 100 microM thymidine almost completely antagonized the ...
Abstract. We show here for the first time that pluripotent hematopoietic stem cells express the CD4 antigen. CD4+ cells isolated from mouse marrow repopulated
TY - JOUR. T1 - Intrinsic Hematopoietic Stem Cell/Progenitor Plasticity. T2 - Inversions. AU - Colvin, Gerald A.. AU - Lambert, Jean François. AU - Moore, Brian E.. AU - Carlson, Jane E.. AU - Dooner, Mark S.. AU - Abedi, Mehrdad. AU - Cerny, Jan. AU - Quesenberry, Peter J.. PY - 2004/4. Y1 - 2004/4. N2 - Traditional concepts indicate that stem cells give rise to progenitor cells in a hierarchical system. We studied murine engraftable stem cells (ESCs) and progenitors in in vitro and found that ESC and progenitors exist in a reversible continuum, rather then a hierarchy. B6.SJL and BALB/c marrow cells were serially cultured with thrombopoietin (TPO), FLT-3 ligand (FLT-3L), and steel factor through cell cycle. Progenitors (high-proliferative potential colony-forming cells (HPP-CFC) and colony-forming unit culture (CFU-c)) and ESC capacity was determined. The cell cycle status of purified lineage negativerhodaminelowHoechstlow stem cells was determined under the same conditions using tritiated ...
L-selectin acts as a homing receptor for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.[2] The receptor is commonly found on the cell surfaces of T cells. Naive T-lymphocytes, which have not yet encountered their specific antigen, need to enter secondary lymph nodes to encounter their antigen. Central memory T-lymphocytes, which have encountered antigen, express L-selectin to localize in secondary lymphoid organs. Here they reside ready to proliferate upon re-encountering antigen. Effector memory T-lymphocytes do not express L-selectin, as they circulate in the periphery and have immediate effector functions upon encountering antigen. High expression of L-selectin on human bone marrow progenitor cells is an early sign of cells becoming committed to lymphoid ...
A methodology for selection of the CD8 cell subset from the peripheral blood and bone marrow mononuclear cells was developed using anti-T8 (CD8) antibody and magnetic microspheres coated with anti-mouse IgG. Following optimization of antibody:cell binding ratio and microsphere:cell ratios, CD8(+)-cells in the peripheral blood and bone marrow were effectively removed, with an overall final recovery of 34.9% +/- 8.6%, and 56% +/- 8.5% respectively with complete recovery of stem cells and very little contamination with effector cells. CD8(+)-depleted cell preparations demonstrated a 3-4 fold increase in CFU-C and CFU-E colony formation over non-depleted preparations when stimulated with G-CSF, GM-CSF or IL-3 and erythropoietin. The largest increase in colony formation was evident when IL-3 was used to stimulate colony formation. Purified autologous CD8+ T-cells or culture supernatant from in vitro cultures of purified autologous CD8+ T-cells added back to CD8 depleted preparations, induced 20%-90%
Hemopoietic precursors with the ability to differentiate into wide varieties of cell types are considered primitive, as are precursors with long-term repopulating ability. Here we study the populations of marrow precursors from which both myeloid and lymphoid lineages are descended shortly after transplantation. Surprisingly, few or none of these precursors show long-term repopulating ability. Equal portions of a mixture of marrow cells from C57BL/6J (B6) and congenic B6-Hbbd Gpi-1a mice are transplanted into a group of recipients. Three weeks later, highly significant correlations between percentages of B6 type T cells, B cells, granulocytes, and platelets in each recipient indicate that many lymphoid and myeloid cells are descended from common precursors. After 4-6 weeks, most correlations between lymphoid and myeloid cells improve, indicating that most or all differentiated cells are descended from common precursors. The more differentiated myeloid-specific precursors found in spleen
Differentiation of Neural-Crest-Derived Intermediate Pluripotent Progenitors into Committed Periodontal Populations Involves Unique Molecular Signature Changes, Cohort Shifts, and Epigenetic Modifications. Smit Jayant Dangaria, Yoshihiro Ito, Xianghong Luan, Thomas G.H. Diekwisch. Stem Cells Dev. 2011 January; 20(1): 39-52. Published online 2010 July 6. doi: 10.1089/scd.2010.0180. PMCID: PMC3128775 ...
PRIME-XV® Mouse Hematopoietic Cell Basal Medium was developed in a collaboration program with a leading stem-cell research facility in the USA to fulfill the need for a serum-free, cost-effective medium for in vitro culture and expansion and self-renewal of murine hematopoietic progenitor cells (mHPCs).. PRIME-XV Mouse Hematopoietic Cell Basal Medium is:. ...
Confluent monolayers of LLC-MK2 cells used in FFU reduction assays were exposed to increasing concentrations of peptide before measuring selleck chemicals Tofacitinib mitochondrial reductase activity using an MTT mitochondrial reductase activity assay (Figure 3). When we initially performed these assays to exactly mimic the focus forming unit assay by waiting five days after peptide exposure, we saw no evidence of toxicity at any concentration of any peptide (data not shown). However, we found that a shorter post-exposure incubation time revealed a subtle toxicity on the part of one of the peptides. Apparently, waiting more than 24 h post-exposure gives the cells a chance to recover and conceals this effect. At 24 h post-exposure, DN57opt was found to be mildly toxic to cells at 40 ��M (one-way ANOVA with Dunnets post hoc test, P=0.. 0004, N=18), so only inhibitory data using lower, nontoxic concentrations was considered. Peptides DN57optscr, 1OAN1, and 1OAN1scr were not toxic at any ...
Stem cells are distinctive cells that can differentiate into any form of the cell of the body owing to its property of totipotency. Hence, they are a continuous resource of the specialized cells that built the organs and tissues.
Caltag Medsystems offer the following services: custom antibodies, angiogenesis services, drug discovery, stem cell assays, genotyping, custom MHC tetramers, flow cytometry, immunoassays, medical device testing and protein analysis.
Caltag Medsystems offer the following services: custom antibodies, angiogenesis services, drug discovery, stem cell assays, genotyping, custom MHC tetramers, flow cytometry, immunoassays, medical device testing and protein analysis.
TY - JOUR. T1 - Suppression and potentiation of mouse hematopoietic progenitor cell proliferation by ouabain. AU - Spivak, J. L.. AU - Misiti, J.. AU - Stuart, R.. AU - Sharkis, S. J.. AU - Sensenbrenner, L. L.. PY - 1980. Y1 - 1980. N2 - Clonal assays for CFU-S, CFU-C, BFU-E, and CFU-E were employed to evaluate the effect of ouabain on the proliferation of mouse hematopoietic progenitor cells. Preincubation of bone marrow cells with ouabain at concentrations of 10-6-10-12 M suppressed the proliferation of CFU-S as measured by the spleen colony assay. At 10-9 M ouabain, spleen colony formation was inhibited by more than 95%. When included in soft agar cultures of bone marrow cells, ouabain suppressed the proliferation of CFU-C in a complex fashion. At 10-4 M ouabain, colony formation was suppressed by 70%, while at 10-6 M ouabain, CFU-C proliferation was normal. At 10-9 M ouabain, however, the number of colonies formed was only 70% of normal, and complete recovery was not obtained at 10-12 M ...
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of normal mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
TY - JOUR. T1 - Association of cell cycle expression of Ia-like antigenic determinants on normal human multipotential (CFU-GEMM) and erythroid (BFU-E) progenitor cells with regulation in vitro by acidic isoferritins. AU - Lu, L.. AU - Broxmeyer, H. E.. AU - Meyers, P. A.. AU - Moore, M. A.. AU - Thaler, H. T.. PY - 1983. Y1 - 1983. N2 - An association has been established between human Ia-like antigenic determinants, expression during DNA synthesis on multipotential (CFU-GEMM) and erythroid (BFU-E) progenitor cells, and the regulatory action of acidic isoferritins in vitro. Treatment of human bone marrow cells with monoclonal anti-Ia (NE1-011) plus complement inhibited colony formation of CFU-GEMM and BFU-E by 50%-70%. Reduction of colonies was similar whether bone marrow cells were exposed to anti-Ia plus complement, high specific activity tritiated thymidine (3HTdr), or acidic isoferritins. No further decrease was apparent with 3HTdr or acidic isoferritins after Ia-antigen+ CFU-GEMM or BFU-E ...
Cortical (cTEC) and medullary (mTEC) thymic epithelial cells establish key microenvironments for T-cell differentiation and arise from thymic epithelial cell progenitors (TEP). However, the nature of TEPs and the mechanism controlling their stemness in the postnatal thymus remain poorly defined. Using TEC clonogenic assays as a surrogate to survey TEP activity, we found that a fraction of cTECs generates specialized clonal-derived colonies, which contain cells with sustained colony-forming capacity (ClonoTECs). These ClonoTECs are EpCAM+MHCII-Foxn1lo cells that lack traits of mature cTECs or mTECs but co-express stem-cell markers, including CD24 and Sca-1. Supportive of their progenitor identity, ClonoTECs reintegrate within native thymic microenvironments and generate cTECs or mTECs in vivo. Strikingly, the frequency of cTECs with the potential to generate ClonoTECs wanes between the postnatal and young adult immunocompetent thymus, but it is sustained in alymphoid Rag2-/-Il2rg-/- counterparts. ...
TY - JOUR. T1 - Overexpression of murine TSLP impairs lymphopoiesis and myelopoiesis. AU - Osborn, Mark J.. AU - Ryan, Patricia L.. AU - Kirchhof, Nicole. AU - Panoskaltsis-Mortari, Angela. AU - Mortari, Frank. AU - Tudor, Kim Sue R S. PY - 2004/2/1. Y1 - 2004/2/1. N2 - The role of thymic stromal cell-derived lymphopoietin (TSLP) in regulating hematopoiesis is poorly characterized, so we investigated its regulatory effects in vivo using TSLP transgenic mice. Overexpression of TSLP disrupted hematopoietic homeostasis by causing imbalances in lymphopoiesis and myelopoiesis. Mice harboring a TSLP transgene had 5- to 700-fold fewer B and T precursors and no detectable pre-B lymphocyte colony-forming activity in the marrow or spleen. Conversely, TSLP transgenic mice possessed 15 to 20 times more spienic myeloid precursors than their littermates, and progenitor activity of the granulocyte-erythrocyte-macrophage-megakaryocyte colony-forming units was significantly elevated. The arrest in lymphopoiesis ...
Hotair is a member of the recently described class of noncoding RNAs called lincRNA (large intergenic noncoding RNA). Various studies suggest that Hotair acts regulating epigenetic states by recruiting chromatin-modifying complexes to specific target sequences that ultimately leads to suppression of several genes. Although Hotair has been associated with metastasis and poor prognosis in different tumor types, a deep characterization of its functions in cancer is still needed. Here, we investigated the role of Hotair in the scenario of epithelial-to-mesenchymal transition (EMT) and in the arising and maintenance of cancer stem cells (CSCs). We found that treatment with TGF-1 resulted in increased Hotair expression and triggered the EMT program. Interestingly, ablation of Hotair expression by siRNA prevented the EMT program stimulated by TGF-1, and also the colony-forming capacity of colon and breast cancer cells. Furthermore, we observed that the colon CSC subpopulation (CD133(+)/CD44(+)) ...
TY - JOUR. T1 - Functional p85α gene is required for normal murine fetal erythropoiesis. AU - Huddleston, Hannah. AU - Tan, Bailin. AU - Yang, Feng Chun. AU - White, Hilary. AU - Wenning, Mary Jo. AU - Orazi, Attilio. AU - Yoder, Mervin C.. AU - Kapur, Reuben. AU - Ingram, David A.. PY - 2003/7/1. Y1 - 2003/7/1. N2 - In vitro studies suggest that activation of class IA phosphatidyiinositol 3 (PI-3) kinase is necessary for normal erythroid cell development. However, when class IA PI-3 kinase-deficient mice were generated by a targeted deletion of the p85α regulatory subunit, fetal erythropoiesis was reportedly unaffected. Given the discrepancies between these studies, we performed a more detailed in vivo analysis of class IA PI-3 kinase-deficient embryos. Day-14.5 p85α-/- embryos are pale with a marked reduction of mature erythrocytes in their peripheral blood. Further, the absolute number and frequency of both early (erythroid burst-forming unit [BFU-E]) and late erythroid progenitors ...
1. Colony-stimulating activity appeared in the perfusate of the isolated rat liver during perfusions with either whole rat blood, rat plasma or an artificial perfusate of Eagles medium and albumin.. 2. Dialysable inhibitors of colony formation were also released during perfusions.. 3. Colony-stimulating activity in artificial perfusate could be enhanced by the addition of rat plasma in vitro. Concentrations of cycloheximide that inhibited albumin synthesis by the liver did not inhibit the release of colony-stimulating activity. ...
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Evaluating the function of an individual hematopoietic stem cell (HSC) is a difficult and important problem. The functional ability per HSC, as well as the HSC concentration, was measured with minimal disruption to the cells in vivo using the new competitive dilution assay. Distribution of HSC into recipients was modeled based on Poisson probabilities. Predictions of donor contributions from models assuming different levels of donor HSC functional ability and concentration were compared to actual observations. The model with the least difference between predictions and observations was accepted. In BALB/ cBy (BALB) mice, models assuming equal functional ability of HSC from the same donor fit extremely well with actual observations, suggesting that all HSC are functionally homogeneous at any particular time point during development or aging. Relative HSC functional ability per cell declined during development, so that a fetal HSC had 1.6 to 3.0 times the functional ability of a young adult
AABB Hematopoietic Progenitor Cell (HPC) activities include educational programs, publications and accreditation for HPC programs.. The list of AABB Accredited HPC Facilities specifies those HPC facilities, in the US and throughout the world, which have attained AABB accreditation. These facilities are responsible for procuring, processing and storing hematopoietic progenitor cells that can be used for transplantation.. ...
Zeisberger, S. M., Zoller, S., Riegel, M., Chen, S., Krenning, G., Harmsen, M. C., ... Zisch, A. H. (2010). Optimization of the culturing conditions of human umbilical cord blood-derived endothelial colony-forming cells under xeno-free conditions applying a transcriptomic approach. Genes to cells, 15(7), 671-687. https://doi.org/10.1111/j.1365-2443.2010.01409.x ...
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The balance between endothelial injury and repair in childhood is poorly understood. We examined this relationship in healthy children, in adults, and in children with familial hypercholesterolemia (FH). Circulating endothelial cells (CECs) wer
Effect of GPC3 on cell proliferation and clonogenic capacity of liver CD90+GPC3+CSCs.(A) Cell proliferation was assessed after GPC3 knockdown in PLC CD90+GPC3+
RATIONALE : Laboratory-treated T cells may stimulate the immune system in different ways and stop tumor cells from growing. Drugs used in chemotherapy,
Clonogenic assay allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate. - Clonogenic Assay - AbVideo™ - Support - Abnova
Chitra Venugopal is the author of this article in the Journal of Visualized Experiments: Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
The second problem with antibiotics is that they interfere with detection. QC Microbiology tells us their tests are sensitive to 1 colony-forming unit (CFU) in the sample, which means if there is 1 CFU in a 40mL sample bottle, theyll find it. But 1 CFU/40mL is 25 CFU/L... and for a 12,000L bioreactor, you need a contamination of ~300,000 CFUs in order for QC to detect contamination ...
thymodepressin: a synthetic peptide, promoted take of transplanted hemopoietic precursor cells in the bone marrow of irradiated mice
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EpiCult-B media can assay for the presence of mammary precursor cells using either the mammary colony-forming cell (Ma-CFC) ... Methylcellulose-based medium is widely used to detect and quantify hematopoietic progenitor cells in the colony-forming unit ( ... and computer system designed specifically for automated imaging and counting of hematopoietic colonies in colony-forming unit ( ... STEMCELL Technologies Media for Hematopoietic Colony Assays MegaCult - Detect Megakaryocyte Progenitor Cells , STEMCELL ...
In this assay system, stromal cells were referred to as colony-forming unit-fibroblasts (CFU-f). The first clinical trials of ... The majority of modern culture techniques still take a colony-forming unit-fibroblasts (CFU-F) approach, where raw unpurified ... Siminovitch L, Mcculloch EA, Till JE (December 1963). "The distribution of colony-forming cells among spleen colonies". Journal ... "Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method". ...
Presentation of End User Labeling Information for Musculoskeletal Implants F2944-20 Practice for Automated Colony Forming Unit ... CFU) Assays-Image Acquisition and Analysis Method for Enumerating and Characterizing Cells and Colonies in Culture F2945-18 ... Guide for Forms Used for Search and Rescue F1768-97(2020) Guide for Using Whistle Signals During Rope Rescue Operations F1772- ... and Solid Form-in-Place Gasket Materials F2379-04(2016) Test Method for Energy Performance of Powered Open Warewashing Sinks ...
"Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: comparison with manual ... A colony-forming unit (CFU, cfu, Cfu) is a unit used in microbiology to estimate the number of viable bacteria or fungal cells ... Instead of colony-forming units, the parameters Most Probable Number (MPN) and Modified Fishman Units (MFU)[citation needed] ... Expressing results as colony-forming units reflects this uncertainty. The purpose of plate counting is to estimate the number ...
... colony-forming units assay MeSH E05.200.500.383.910 - tumor stem cell assay MeSH E05.200.500.385 - cytogenetic analysis MeSH ... drug screening assays, antitumor MeSH E05.337.550.200.800 - tumor stem cell assay MeSH E05.337.550.200.900 - xenograft model ... drug screening assays, antitumor MeSH E05.200.500.388.930 - tumor stem cell assay MeSH E05.200.500.410 - electroporation MeSH ... antitumor assays MeSH E05.337.550.200.900.830 - subrenal capsule assay MeSH E05.337.550.400 - microbial sensitivity tests MeSH ...
... colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue ... CFU-E stands for Colony Forming Unit-Erythroid. It arises from CFU-GEMM (via BFU-E, which stands for "erythroid burst-forming ... Marley SB, Lewis JL, Goldman JM, Gordon MY (June 1996). "Abnormal kinetics of colony formation by erythroid burst-forming units ... Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit, Terszowski G. et al. Blood ...
"Antiproliferative activity of Titanocene Y against tumor colony-forming units". Anticancer Drugs. 18 (3): 317-321. doi:10.1097/ ... "Analyses of Titanocenes in the spheroid-based cellular angiogenesis assay". Toxicol In Vitro. 22 (2): 531-534. doi:10.1016/j. ... Titanocene Y can be given in the mouse in high dosages and it shows generally mild toxicity in the form of diarrhea. Titanocene ...
Virtual colony forming units, or CFUv, is defined in the original VCC publication and its definition is repeated here: CFUv was ... Virtual colony count (VCC) is a kinetic, 96-well microbiological assay originally developed to measure the activity of ... After spreading, the agar plates are then incubated overnight and the number of colony forming units (CFU) is counted the next ... Because this procedure requires no actual colony formation or colony counting, it is termed "virtual colony count". Thus far ...
The microbe, or the inoculating agent, must come from the same colony-forming unit, and must be at the correct concentration. ... There are three main reagents necessary to run this assay: the media, an antimicrobial agent, and the microbe being tested. The ... For verification, the positive control is plated in a hundred fold dilution to count colony forming units. The microbes ... Antimicrobial toxicity can come in many forms, such as immune hypersensitivity and off-target toxicity. ...
106 colony forming units (CFU) of C. butyricum MIYAIRI 588 (as active agent). CBM 588 does not establish permanently in the gut ... has been demonstrated by PCR assay. ... Clostridium butyricum is a strictly anaerobic endospore-forming ...
... it can be generally assumed that each cell will give rise to a single colony or Colony Forming Unit (CFU). The colonies can ... for instance the Luria-Delbrück experiment or the gentamicin protection assay). In addition, the enumeration of colonies on ... instead of obtaining single colonies that can be counted, a so-called "lawn" will form: thousands of colonies lying over each ... Additionally, plating is the slowest method of all: most microorganisms need at least 12 hours to form visible colonies. ...
The infectious dose of Campylobacter ranges between 1000-10,000, but even 500-800 colony forming units (CFU) has also been ... can be diagnosed by isolation of the organism from faeces and identification by growth-dependent tests, immunological assays, ... In the year 1886, Theodor Escherich established that spiral form bacteria were noted in the stool specimen and large intestinal ... Campylobacter coli is a Gram-negative, microaerophilic, non-endospore-forming, S-shaped bacterial species within genus ...
... in which colony forming units (CFU) on selective media are counted. Cultivating methods have several disadvantages. Culture- ... enzyme-linked immunosorbent assay (ELISA)). The well-known PCR is a powerful tool in identifying and even quantifying the ... This underestimation is likely to be signified for the quantification of bioaerosol, since colony counts of airborne microbes ... irrelevance of sampling units to human exposure measurement; multiplicity and variability of composition, etc.). To enable ...
Pure yellow colonies formed within 24 hours. The identification of the bacterium as Corynebacterium rathayi was insufficiently ... A functional tunicamycin gene cluster has been identified consisting of 14 genes composing 2 separate transcriptional units. ... were distinguishable from not only Corynebacterium rathayi but other phytopathogenic coryneforms through immunological assays. ... Colony morphology on 523M agar is convex, smooth, mucoid with yellow, rose-orange, or pink pigmentation. Anguina sp. (seed gall ...
Siminovitch L, Mcculloch EA, Till JE (December 1963). "THE DISTRIBUTION OF COLONY-FORMING CELLS AMONG SPLEEN COLONIES". Journal ... "Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method". ... "Neurons derived from radial glial cells establish radial units in neocortex". Nature. 409 (6821): 714-20. doi:10.1038/35055553 ... A: Stem cell colonies that are not yet differentiated. B: Nerve cells, an example of a cell type after differentiation. ...
This group has published guidelines about the actions to be taken to limit the number of colony forming units (i.e., the " ... disease by serological assays". BMC Infectious Diseases. 15: 163. doi:10.1186/s12879-015-0903-2. PMC 4383209. PMID 25887275. ... The tabled figures are for total aerobic plate count, cfu/ml at 30 °C (minimum 48 hours incubation) with colony count ... of freshwater sites by PCR hybridization assay.[citation needed] Legionella bacteria themselves can be inactivated by UV light ...
The other reliable identification test at genus level is chromosomal DNA transformation assay. In this assay, a naturally ... Acinetobacter is frequently isolated in nosocomial infections, and is especially prevalent in intensive care units, where both ... In healthy individuals, Acinetobacter colonies on the skin correlate with low incidence of allergies; Acinetobacter is thought ... Gene-silencing antisense oligomers in a form called peptide-conjugated phosphorodiamidate morpholino oligomers have also been ...
The unit of measurement is cfu/ml (or colony forming units per millilitre) and relates to the original sample. Calculation of ... samples contain a variety of components that can interfere with the ATP assay. The plate count method relies on bacteria ... Colonies that develop in the body of the medium can be counted by eye after incubation. The total number of colonies is ... growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate ...
The complexes formed by the looping of the DNA are known as chromatin. The basic structural unit of chromatin is the nucleosome ... 3. Assay for transposase accessible chromatin sequencing (ATAC-seq) is used to look in to regions that are nucleosome free ( ... H2BK5ac elevated cell viability while the number of colonies and migration are impeded. The histone mark acetylation can be ... They are formed during the first stage of pregnancy and are the first cells to differentiate from the fertilized egg. or, after ...
Colony-forming unit-megakaryocyte (CFU-Meg) Colony-forming unit-basophil (CFU-B) Colony-forming unit-eosinophil (CFU-Eos) The ... A cobblestone area-forming cell (CAFC) assay is a cell culture-based empirical assay. When plated onto a confluent culture of ... Colony-forming unit-lymphocyte (CFU-L) Colony-forming unit-erythrocyte (CFU-E) Colony-forming unit-granulocyte-macrophage (CFU- ... which is a cell counting unit.) There are various kinds of HSC colony-forming units: Colony-forming unit-granulocyte- ...
This time his legislation took the form of an amendment (S.AMDT.418) to H.R. 47, the "Statue of Liberty-Ellis Island ... Secretary of the Treasury James A. Baker III presided over the striking ceremony held at the San Francisco Assay Office. ... The sets sold out at the issue limit of 75,000 units. In fewer than twenty known sets, the Sacagawea Dollars do not have a ... above the eagle are thirteen five-pointed stars representing the Thirteen Colonies. The reverse is inscribed with the phrases ...
A form of replicate known as a dye flip can be performed to control for dye artifacts in two-channel experiments; for a dye ... Second, technical replicates (two RNA samples obtained from each experimental unit) help to ensure precision and allow for ... Microarray data is difficult to exchange due to the lack of standardization in platform fabrication, assay protocols, and ... and ant colony optimization. Input data for class prediction are usually based on filtered lists of genes which are predictive ...
It has been shown to cure cats infected with Feline infectious peritonitis (FIP), a feline form of coronavirus with a 96% cure ... In a statement to the journal Nature Biotechnology in February 2020, US National Institutes of Health Viral Ecology Unit chief ... Niclosamide was identified as a candidate antiviral in an in vitro drug screening assay done in South Korea. Protease ... Mavrilimumab is a human monoclonal antibody that inhibits human granulocyte macrophage colony-stimulating factor receptor (GM- ...
Titers of plaques can be found by diluting the samples and counting plaque-forming units (PFUs). Biochemical tests such as ... Plaque assays consist of pouring a soft agar solution with an indicator strain onto an agar plate. The indicator strain should ... In addition, none of the colonies containing the T12 genome was negative for speA, and therefore, the conclusion was drawn that ... "Plaque Assay Protocols". Microbe Library. American Society for Microbiology. Archived from the original on 30 November 2012. ...
After the development stage today Arvohaler is a worldwide patented multi-unit dose dry powder inhalation (DPI) device ... 50 mcg/250 mcg and 50/100 mcg inhalation powder forms. Development of Cyplos Arvohaler products were started in 2006, ... "Method Development and Validation of Simultaneous Determination of Assay of Salmeterol Xinafoate and Fluticasone Propionate in ... and Functional Similarity of Biosimilar Granulocyte Colony Stimulating Factor to its Reference Product". Pharmaceutical ...
Each unit of β-galactosidase consists of five domains; domain 1 is a jelly-roll type β-barrel, domain 2 and 4 are fibronectin ... The β-galactosidase assay is used frequently in genetics, molecular biology, and other life sciences. An active enzyme may be ... The active ebg enzyme is an aggregate of ebgA -gene and ebgC-gene products in a 1:1 ratio with the active form of ebg enzymes ... After a time, certain colonies began to grow. However, the EbgA protein is an ineffective lactase and does not allow growth on ...
Depending on the nature of the Detection system assays can be based on: Colony forming or virtual colony count: e.g. by ... number of assays done per unit time (usually expressed as per hour) etc. Organizations or laboratories that perform Assays for ... DNase footprinting assay Filter binding assay Gel shift assay Bicinchoninic acid assay (BCA assay) Bradford protein assay Lowry ... Chemotaxis assay Secretion assays Apoptosis assays such as the DNA laddering assay, the Nicoletti assay, caspase activity ...
Colony-forming unit Blood culture Microbial dark matter Microbial Food Cultures Screening cultures Sputum culture Synchronous ... Liquid cultures are ideal for preparation of an antimicrobial assay in which the experimenter inoculates liquid broth with ... Upon incubation, colonies will arise and single cells will have been isolated from the biomass. Once a microorganism has been ... Upon incubation, colonies will arise and single cells will have been isolated from the biomass. Once a microorganism has been ...
The most common forms of β-glucans are those comprising D-glucose units with β-1,3 links. Yeast and fungal β-glucans contain 1- ... An assay to detect the presence of (1,3)-β-D-glucan in blood is marketed as a means of identifying invasive or disseminated ... Colony-stimulating factors. *G-CSF *Filgrastim / Lipegfilgrastim / Pegfilgrastim. *Lenograstim. *Eflapegrastim. *GM-CSF * ... β-glucans form a natural component of the cell walls of bacteria, fungi, yeast, and cereals such as oat and barley. Each type ...
From there, Model C was born with an adjustment to the wavelength resolution which ended up having three units of it produced. ... A certain chemical reaction within a solution may occur in a forward and reverse direction, where reactants form products and ... and can also be used as a method to create optical assays of a compound. Spectrophotometric data can also be used in ... 21-119 Following this testing the sample at 420 nm for specific interaction with ONPG and at 595 for a Bradford Assay the ...
On the second day of battle, a unit of the XIX Corps was forced to halt when it ran out of gasoline.[39] One of the major ... Shortening gasoline to gas, which happens often, causes confusion with various forms of gaseous products also used as ... though this varies based on the crude oil assay. ... navy to protect the shipping of raw materials from its colonies ... However, before feeding those units, the naphtha needs to be split into light and heavy naphtha. Straight-run gasoline can be ...
In 1966 an international team, the Smallpox Eradication Unit, was formed under the leadership of an American, Donald Henderson. ... "Chicken pox or smallpox in the colony at Sydney Cove in April, 1789". Radio National. 17 September 2010.. ]. ... Serologic tests and enzyme linked immunosorbent assays (ELISA), which measured Variola virus-specific immunoglobulin and ... There were two forms of the smallpox virus. Variola major was the severe and most common form, with a more extensive rash and ...
... which may be separated from other colonies or melded together into a "lawn". The size, color, shape and form of a colony is ... "Exobiological Unit" of ERA on EURECA I". Adv. Space Res. 16 (8): 105-18. Bibcode:1995AdSpR..16..105V. doi:10.1016/0273-1177(95) ... A basic form of contagion theory was proposed by Persian physician Ibn Sina (known as Avicenna in Europe) in The Canon of ... Frequent hand washing remains the most important defense against the spread of unwanted organisms.[37] There are other forms of ...
One form is scanner drift, caused by the superconducting magnet's field drifting over time. Another form is changes in the ... Pharmacological fMRI, assaying brain activity after drugs are administered, can be used to check how much a drug penetrates the ... It is also expensive to maintain a colony of larger animals such as the macaque.[53] ... but not as good a resolution as invasive procedures such as single-unit electrodes. While typical resolutions are in the ...
... the disease progresses directly from the acute form to a symptomatic clinical form of chronic Chagas disease.[5][9] ... Even when colonies of insects are eradicated from a house and surrounding domestic animal shelters, they can re-emerge from ... including all units collected by the American Red Cross, which accounts for 40% of the U.S. blood supply.[38][39] The Chagas ... and Panstrongylus megistus in laboratory assays". Rev Panam Salud Publica. 7 (6): 384-8. doi:10.1590/S1020-49892000000600005. ...
... is also linked to the tube nucleus and the interconnected sperm and the tube nucleus form the "male germ unit".[14] ... "Beekeeping in Africa: Colony life and social organization". FAO.. *^ Fertilization in protozoa and metazoan animals: cellular ... Pistil feeding assays in which plants were fed diphenyl iodonium chloride (DPI) suppressed ROS concentrations in Arabidopsis, ... Some relatively unusual forms of reproduction are:[37][38]. Gynogenesis: A sperm stimulates the egg to develop without ...
On the second day of battle, a unit of the XIX Corps was forced to halt when it ran out of gasoline.[39] One of the major ... because various forms of gaseous products are also used as automotive fuel, such as compressed natural gas (CNG), liquefied ... though this varies based on the crude oil assay. ... navy to protect the shipping of raw materials from its colonies ... alkylate is produced in an alkylation unit, using isobutane and olefins as feedstocks. Finished alkylate contains no aromatics ...
Most slippage results in a change of just one repeat unit, and slippage rates vary for different allele lengths and repeat unit ... An archaic form of splicing preserved in Zebrafish is known to use microsatellite sequences within intronic mRNA for the ... Colonies are then developed, and screened with fluorescently-labelled oligonucleotide sequences that will hybridize to a ... in a gel assay), thus only one allele is amplified (from the non-mutated sister chromosome), and the individual may then ...
Main article: Colony collapse disorder. In apiculture in the United States, HFCS is a honey substitute for some managed honey ... Assays to detect adulteration with HFCS use differential scanning calorimetry and other advanced testing methods.[16][17] ... Some is processed into HFCS 90 by liquid chromatography, then mixed with HFCS 42 to form HFCS 55. The enzymes used in the ... IU = International units. Percentages are roughly approximated using US recommendations for adults.. Source: USDA Nutrient ...
Main article: Colony collapse disorder. In apiculture in the United States, HFCS is a honey substitute for some managed honey ... Assays to detect adulteration with HFCS use differential scanning calorimetry and other advanced testing methods.[12][13] ... The most common forms of HFCS used for food and beverage manufacturing contain fructose in either 42% ("HFCS 42") or 55% ("HFCS ... IU = International units. †Percentages are roughly approximated using US recommendations for adults. Source: USDA Nutrient ...
Many microorganisms affect anaerobic digestion, including acetic acid-forming bacteria (acetogens) and methane-forming archaea ... are fed into a crusher unit, where the mixture is conflated with a small amount of water. The mixture is then fed into the bio- ... The first known anaerobic digester was built in 1859 at a leper colony in Bombay in India.[129] In 1895, the technology was ... Direct measurements of biomethanation potential are also made using gas evolution or more recent gravimetric assays.[65] ...
The leaves (called dandelion greens) can be eaten cooked or raw in various forms, such as in soup or salad. They are probably ... crops and its importance for colony development". Botany. 90 (7): 545-555. doi:10.1139/b2012-049.. ... Moreover, the diverse pharmacological activities of dandelion or individual compounds isolated thereof have only been assayed ... IU = International units. †Percentages are roughly approximated using US recommendations for adults. Source: USDA Nutrient ...
... which may be separated from other colonies or melded together into a "lawn". The size, color, shape and form of a colony is ... "Exobiological Unit" of ERA on EURECA I". Adv. Space Res. 16 (8): 105-18. Bibcode:1995AdSpR..16..105V. doi:10.1016/0273-1177(95) ... A basic form of contagion theory dates back to medicine in the medieval Islamic world, where it was proposed by Persian ... Frequent hand washing remains the most important defense against the spread of unwanted organisms.[29] There are other forms of ...
Detachment assays with NaIO4, proteinase K, or DNase result in 38%, 98%, and 100% detachment, respectively. The high level of ... The ability to adhere to medical devices and subsequently form biofilms is a major virulence factor associated with S. ... 2007). "Persistent strains of coagulase-negative staphylococci in a neonatal intensive care unit: virulence factors and ... 16 conventional growth tests including: colony pigment, DNase, alkaline phosphatase, ornithine decarboxylase, urease, acetoin ...
Of the 800 species of ticks, 40-64 species (across 10 genera) have been reported as causing a form of toxosis.[53][54][55][56] ... At the Royal North Shore Hospital in Sydney the Tick-borne Diseases Research Unit continues its study into this issue. Cases of ... Stone BF, Cowie MR , Kerr JD and Binnington KC (1982) Improved toxin/antitoxin assays for studies on the Australian paralysis ... The 30-day engorgement time is derived from laboratory culture colonies.) Under natural conditions, the time taken for an adult ...
Quality filtering of the reads can deliver higher raw accuracy reads which when aligned to form color contigs can deliver ... Therefore, since each color represents two nucleotides in which the second base of each dinucleotide unit constitutes the first ... a bead or a planar solid surface Amplification of DNA fragments on the solid surface using PCR and making polymerase colonies ... the use of emulsion PCR to generate millions of clonally amplified beads which one could perform these repeated ligation assays ...
... a unit used only by mints. The tower pound was abolished in the 16th century.) The silver penny remained the primary unit of ... Assaying is now done by the Worshipful Company of Goldsmiths on behalf of HM Treasury. The 1p and 2p coins from 1971 are the ... The 1p, 2p, 5p, 10p, 20p and 50p coin designs post 2008 each depicts a part of the Royal Shield, and form the whole shield when ... Chalmers, Robert (1893). A History of Currency in the British Colonies. London, UK: Her Majesty's Stationery Office. p. 110. ...
A colony of Australian sea lions reside at Dyer Island and a colony of New Zealand fur seals (Arctocephalus forsteri) reside at ... A sandy, low-lying island formed on a base of aeolianite limestone, Rottnest is an A-class reserve, the highest level of ... Rottnest Island Authority accommodation options include 291 villas, units and cottages which sleep 4, 6 or 8 people and which ... provisionally dated by aspartic acid racemisation assay of land snails to greater than 50 ka". Australian Archaeology. 49 (49 ...
Each of these units is valued at one one-hundredth of its corresponding base unit, such as the dollar, euro, peso, etc. ... assay A test to ascertain the weight and purity of a coin. attribution An identifier of a coin, such as date, mint, ... ingot A bar of pure metal formed by pouring the molten metal into a mould. It may be stamped with its weight and purity. ... Spanish dollar A coin issued in Spain and its colonies from 1497 to 1864, equal to eight reales. It was legal tender in the ...
... established a self-governing colony, the Massachusetts Bay Colony, and they decided to settle to the New World. John Winthrop ... There were 1,502 units in the development on 50 acres (200,000 m2) of land. In 1966, the Columbia Point Health Center opened ... For the first time in America, inoculation was tried; it causes a mild form of the disease. Inoculation was itself very ... Town officials in colonial Boston were chosen annually; positions included selectman, assay master, culler of staves, fence ...
A first round of PCR using these primer sets is performed and two double stranded DNA duplexes are formed. A second round of ... Plasmids are then isolated for colonies which show improved traits, and are then used as templates the next round of ... This results in the reconstruction of complete proteins by assembly of modules encoding different structural units.[page needed ... Also highly specific low throughput screening assays are increasingly applied in place of monumental screening and selection ...
div class=section, ,h2,Background,/h2, ,p,Hematopoietic cell culture assays are used to detect the proliferation and ... The colony-forming unit (CFU) assay is an in vitro functional assay for enumerating multipotential and lineage-committed ... Matsumura-Takeda K, Kotosai K, Ozaki A, Hara H, Yamashita S. Rat granulocyte colony-forming unit (CFU-G) assay for the ... thus forming a colony of mature cells. CFU assays are used to quantitate and characterize hematopoietic progenitors from ...
Home/Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony ... Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting). ... Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting). ... To get support on Synoptics systems you can visit our support helpdesk, send us an email, or fill in an online form:. ...
Colony-forming unit assay. For enumeration of blood colony-forming cells (CFU), aliquots of bone marrow cells were incubated in ... i No significant difference in colony-forming units of progenitor stem cells was observed between untreated, control and ... bone marrow progenitor cells extracted from mice treated with CRIT did not reveal a significant change in colony-forming units ... ion in a monomeric form, are lost in MKT4 and Tf-TC17,18. Thus, sequestration of TC in the hydrophobic region of NM may help ...
... functional assays, including serial BM transplantation, reveal that the self-renewal and competitiveness of HSCs are impaired. ... Colony-forming unit assay. Clonogenic progenitors were determined in methylcellulose colony assay medium (MethoCult GF M3434, ... n = 4. e Colony-forming unit (CFU) assay of total BM cells. n = 3. f, g Representative FACS analysis (f) and absolute cell ... 1d; Supplementary information, Figure S2L). We also performed colony-forming unit (CFU) assays to evaluate the function of BM ...
Colony forming unit (CFU) assay. The CFU assay was performed by diluting an overnight culture 1:100 in a total volume of 10 ml ... The number of colonies in each plate was counted and the CFU/ml (number of colonies/volume of inoculation×dilution) was ... FRET assay. The FRET measurements were performed as previously described [43, 46]. Briefly, E. coli cells were grown in ... Microfluidics assay. The measurement of the chemotactic drift was performed as previously described [52]. Briefly, E. coli ...
Assay for colony-forming units. Assays for t-CFUs provide a convenient means of assessing the proliferative capacity that MSCs ... CFU assay) as a guide to the growth potential of the cultures (Figure 1B). For the traditional colony-forming unit assay (t-CFU ... Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the ... B) t-CFU assays were performed after the 12 days incubation in the medium indicated. (C) t-CFU assays and sc-CFU assays were ...
Colony-forming unit assay. Freshly harvested mouse bone marrow cells or patient splenocytes (2 × 104 cells/mL) were assayed for ... colony forming units (CFU) in 0.9% methylcellulose IMDM containing 30% FBS, glutamine (10−4 mol/L), β-mercaptoethanol (3.3 × 10 ... In the presence of GM-CSF, the colony-forming capabilities of PTPN11E76K/+ myeloid progenitors were more sensitive to #220-324 ... Colonies were enumerated 7 days later and normalized against the number of colonies derived from WT control cells without #220- ...
The BLOC-3 complex is comprised of the two sub-units HPS1 and HPS4 and exhibits guanine-nucleotide exchange factor (GEF) ... The BLOC-3 complex is comprised of the two sub-units HPS1 and HPS4 and exhibits guanine-nucleotide exchange factor (GEF) ... Infection and Colony-Forming Unit Assay (CFU). Over-night cultures of Salmonella strains were diluted 1:20 in 0.3 M NaCl LB- ... Secondly, we used a colony-forming unit assay to assess whether S. Typhimurium survival is affected in macrophages with ...
Culture Colony-Forming Unit Assay.. Frontoparietal bones, femora, and brains of five mice were ground using a mortar and pestle ... pathway and one or more unidentified lectins determine in part the recovery of colony-forming units from the BM of irradiated ... D) Colony-forming capacity of MNCs from murine brain, skull, and femur. MNCs isolated from brains, skullcaps, and femora of ... The difference in the frequency of colonies in CFU-C assays using MNCs from skulls and femora was not statistically significant ...
... sarA and sarA mediated virulence genes upon myrtenol treatment which is well correlated with results of phenotypic assays. Thus ... was evidenced by trypan blue and Alamar blue assays. Transcriptional analysis unveiled the down-regulation of global regulator ... Colony Forming Unit (CFU) Assay. CFU assay was performed to determine the cell count variation between control and myrtenol ... Mature Biofilm Assay. To determine the effect of myrtenol on mature biofilm, MRSA was allowed to form biofilm on glass slides ...
Colony-forming unit assay. A colony-forming unit (CFU) assay was carried out to show blast elimination in a long-term coculture ... 4C). Furthermore, a specific inhibition of colony formation was observed in CFU assays after 14 days when leukemia blasts were ... Colonies were counted on day 14 and relative inhibition of colony formation calculated in comparison with nonspecific T cells ... IFN-γ enzyme-linked immunospot assay. Peptide recognition was tested in an IFN-γ-enzyme-linked immunospot (ELISpot) assay (23 ...
Colony-forming unit (CFU) assay. To evaluate the capacity of MSC to sustain normal hematopoiesis, a CFU assay was performed ... colony-forming unit-erythroid; CFU-GM: colony-forming unit-granulocyte and macrophage; CFU-Mix: colony-forming units mixed; CFU ... colony-forming unit-erythroid; CFU-GM: colony-forming unit-granulocyte and macrophage; CFU-Total: total colony-forming units; ... total colony-forming units; CFU-MK: colony-forming unit-megakaryocyte; SEM: standard error of mean. ...
Colony forming unit-fibroblast assay. CFU-F assays were performed by plating either 1×105 unselected or 500-5,000 FACS-selected ... MSC can be identified by their ability to form colony forming units-fibroblast (CFU-F) in vitro.2,7 However, these cells are ... The growth of mesenchymal stem cells was monitored by colony formation unit fibroblast (CFU-F) assays. The differentiation of ... could be expanded and used for differentiation assays, indicating that only these colonies are derived from immature MSC. ...
Colony-forming unit assays. Mouse BM cells were harvested via flushing of the long bones with Dulbecco modified Eagle medium ( ... To further confirm the expansion of miR-142−/− MkPs, we employed a colony forming unit-megakaryocyte (CFU-MK) assay that ... G) Left panel, CFU-MK assays demonstrate increased miR-142−/− MK numbers per colony. Scale bars, 50 μm. Right panel, increased ... Colonies containing ,3 MKs were counted as CFU-MKs. Duplicate assays were performed for each mouse. At least two mice were ...
S2E). Furthermore, we also performed a colony-forming unit assay in methylcellulose to determine the number of viable colonies ... Colony-forming unit assay. Nalm6, Jurkat, and 658w WT and casp-3/7−/− cells were plated in 3 ml of RPMI 1640 complete medium in ... Colony-forming units (CFUs) are the average from three experiments each performed in duplicate. ... After 10 to 14 days, colony-forming units were visually counted under a microscope. ...
... functional assays, and mesenchymal stem cell expansion media to improve cell quality and consistency. ... Colony-Forming Unit-Fibroblast (CFU-F) Assay. The colony-forming unit-fibroblast assay can be used to estimate the ... MSCs were originally identified by Alexander Friedenstein as colony forming unit fibroblasts (CFU-F) and osteogenic stem cells. ... MSCs isolated by CD105/Endoglin positive selection demonstrate tri-lineage differentiation and higher colony-forming units- ...
Colony-forming units (CFU) assay. CFU assay was performed by mixing freshly isolated or cultured hematopoietic cells with ... were used for colony-forming assay (n = 4). f Photomicrographs of human hemopoietic cell colonies. Scale bar represented 200 μm ... 5c, d). Colony-forming assay of BM cells showed higher BM CFU counts in hD1R-treated mice than in PBS-treated ones (Fig. 5e). ... The results showed that the total number of colonies and the numbers of burst-forming unit-erythroid (BFU-E), CFU-granulocyte ...
Colony-Forming Unit (cfu) Assay. Fungicidal activity was measured with a cfu assay. Fluconazole-sensitive C. albicans was ... Abbreviations: cHTS, combination high-throughput screening; cfu, colony-forming unit; HSA, highest single agent; PAP, ... 4 A-C) and in vivo in a human tumor xenograft assay in mice (Fig. 4D). The combination was even more effective than paclitaxel ... Dye Efflux Assay. Fluconazole-resistant C. albicans cultures were pretreated with compound(s) for 30 min at 32°C and then ...
Colony-forming Units Assay. A cytologic technique for measuring the functional capacity of stem cells by assaying their ... Each colony (i.e., microbial colony-forming unit) represents the progeny of a single cell in the original inoculum. The method ... Summary of "Colony-Forming Cell Assay Detecting the Co-Expression of JAK2V617F and BCR-ABL1 in the Same Clone: A Case Report." ... Colony-Forming Cell Assay Detecting the Co-Expression of JAK2V617F and BCR-ABL1 in the Same Clone: A Case Report.. 08:00 EDT ...
... a discrepancy is observed between the decreased in vitro erythroid colony formation and the normal or increased number of ... Colony-Forming Units Assay. Erythropoiesis*. Erythropoietin / blood. Hematocrit. Hematopoietic Stem Cells / pathology*, ... Characterization of the erythropoiesis in myelodysplasia by means of ferrokinetic studies, in vitro erythroid colony formation ... and erythroid in vitro colony formation were performed in 24 patients with RA and five patients with RARS. These results were ...
... gel overlay assays as well as disc diffusion assays.. The colony forming units (CFU) assay. The antibacterial activity of LDs ... Antibacterial assays. To evaluate the antibacterial property of purified LDs/LD components, we performed colony forming assays ... C). Representative plates in a colony forming assay, showing bacterial colonies on agar plates streaked with cytosolic extract ... Quantification of colony forming assay in A. Each bar represents the mean number of observed colonies, in three independent ...
Colony-forming units assay‎ (21 F). *. ► Cytogenetics‎ (4 C, 21 F). *. ► Cytological techniques‎ (3 C, 54 F) ... Colonies of Madin-Darby Canine Kidney cells grown in tissue culture.jpg 1,030 × 1,030; 263 KB. ... Astrocyte and Glioblastoma Invasion Assay performed after 3D culturing by MLM.tiff 687 × 536; 363 KB. ...
Colony-forming units assay‎ (21 F). *. ► Cytogenetics‎ (5 C, 22 F). *. ► Cytological techniques‎ (3 C, 52 F) ... Colonies of Madin-Darby Canine Kidney cells grown in tissue culture.jpg 1 030 × 1 030; 263 KB. ... Astrocyte and Glioblastoma Invasion Assay performed after 3D culturing by MLM.tiff 687 × 536; 363 KB. ...
Colony-Forming Unit Assays. Single-cell suspensions from the spleen and BM were prepared at 1 × 106 and 2 × 105 cells/mL, ... The cultures were incubated at 37°C, 5% CO2 in air, and 95% humidity for 8 days after which Colony-Forming Unit Macrophages ( ... 0.001, resp.). Analysis of progenitors Colony Forming Unit-Macrophage (CFU-M) did not reveal major modifications in the BM of ... the numbers of Colony Forming Unit-Macrophage (CFU-M) were determined in 12-day bone marrow (c) and spleen (d) cultures using ...
Colony-Forming Units Assay * Embryo, Mammalian * Fluorouracil / pharmacology * Hematopoiesis * Hematopoietic Stem Cells / ...
PL lots were obtained by combining 2 6-unit PLT pools in additive solution (AS) following a transfusional-based procedure ... Here, we describe a plasma poor pathogen-free platelet lysate obtained by pooling 12 platelet (PLT) units, to produce a ... Colony-forming unit-fibroblast (CFU-F) assay. Colony-forming-unit assay was performed by plating 2 × 105 BM-MNC/ml in D-MEM ... In detail, two pools of six PI-PLT units were connected each other under sterile conditions to obtain a pool of 12 PI-PLT units ...
... these cells grew to have the highest colony-forming unit (CFU) counts. In phenotypic analysis using flow cytometry, similarity ... Colony-Forming Unit (CFU) Assay. Colony-forming ability analysis was modified from our previous study [15]. BMSCs, PDB-MSCs, ... Results of colony-forming unit (CFU) assay and cell proliferation analysis. (a) Quantification of stem cells colony-forming ... PDB-MSCs resulted in better colony-forming abilities than USCs (. , , and , ) (Figure 2(a)). As measured by trypan blue ...
MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays Product Type:. Cell Culture Media and ... ClonaCell™-HY Medium D for Robust Colony Formation During Semi-Solid Cloning Product Type:. Cell Culture Media and Supplements ... Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays Cell Type:. Hematopoietic Stem and Progenitor ...
Colony-forming units (CFU) assay and cell cloning. For colonies, cells were plated at a density of 150 cells/10 cm dish. After ... Staining was performed using 0.3% Oil Red O solution (Sigma-Aldrich). For quantitative as- say, Oil Red O bound to lipid ... Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies, Exp. Hematol. 36 ( ...
Colony-Forming Units Assay * DNA-Binding Proteins / physiology* * Embryonic Stem Cells / metabolism ...
  • The TTDSL has a broad expertise in qPCR, ELISA, protein HPLC, cell culture, and flow cytometry-based assay development. (cincinnatichildrens.org)
  • Homing efficiency analysis was conducted using flow cytometry and colony-forming unit (CFU) assays. (biomedcentral.com)
  • ZAP-70 testing by flow cytometry is a fast and cost-friendly alternative method to molecular assays (Crespo et al. (beckman.com)
  • Studies of the cellular pathogenesis mechanisms of pathogenic yeasts such as Candida albicans , Histoplasma capsulatum , and Cryptococcus neoformans commonly employ infection of mammalian hosts or host cells ( i.e. macrophages) followed by yeast quantification using colony forming unit analysis or flow cytometry. (jove.com)
  • The colony-forming unit (CFU) assay is an in vitro functional assay for enumerating multipotential and lineage-committed hematopoietic progenitor cells through the use of semi-solid culture media. (stemcell.com)
  • Characterization of the erythropoiesis in myelodysplasia by means of ferrokinetic studies, in vitro erythroid colony formation and soluble transferrin receptor. (biomedsearch.com)
  • In refractory anemia (RA) and refractory anemia with ringed sideroblasts (RARS) a discrepancy is observed between the decreased in vitro erythroid colony formation and the normal or increased number of normoblasts in the bone marrow. (biomedsearch.com)
  • To study the in vivo and in vitro erythropoiesis in more detail erythron transferrin uptake (ETU), soluble transferrin receptor (sTfR) and erythroid in vitro colony formation were performed in 24 patients with RA and five patients with RARS. (biomedsearch.com)
  • However no correlation was found between the ETU values and the in vitro erythroid colony formation. (biomedsearch.com)
  • While not generally appreciated, histones and histone fragments are surprisingly bactericidal in in vitro assays ( Hirsch, 1958 ). (elifesciences.org)
  • Hematopoiesis can be recapitulated in vitro by using colony-forming unit (CFU) assays that specifically provide for the expansion of each progenitor type. (aspetjournals.org)
  • Apoptosis induction by p53p-Ant was quantitated by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling assay and Annexin V staining in human glioma cells in vitro and in a syngeneic orthotopic 9L glioma rat model using convection-enhanced delivery in vivo . (aacrjournals.org)
  • The pre-implantation mouse epiblasts obtained from blastocysts have the ground-state naïve pluripotency that can be recapitulated in vitro in the form of embryonic stem cells (ESCs) [ 8 , 9 ]. (biomedcentral.com)
  • In vitro clonogenic assays yielded a normal number of colony-forming units but no growth in cultures without growth factor supplement. (nih.gov)
  • After 20 days of in vitro differentiation, cells revealed typical hematopoietic morphology, CD45 expression, and colony-forming unit (CFU) potential. (biomedcentral.com)
  • and the Colony-Forming Unit (CFU) assay which tests the in vitro growth of progenitor cells [6,7]. (parentsguidecordblood.org)
  • The results of in vitro differentiation assays showed that RAC2 is essential for the survival and differentiation of hematopoietic stem/progenitor cells. (haematologica.org)
  • The bone marrow was used for ex vivo CFU-OB (Colony forming units-Osteoblast) and CFU-AD assays using the same protocol described for the in vitro studies previously. (clinicaltrials.gov)
  • Hematopoietic cell culture assays are used to detect the proliferation and differentiation ability of hematopoietic cells at distinct and successive stages of differentiation, and to measure the frequency of these cells in hematopoietic tissues and purified cell populations. (stemcell.com)
  • These culture media are supplemented with cytokines to promote the proliferation and differentiation of hematopoietic progenitors and their viscosity constrains the clonal progeny of a single progenitor cell to a small area, thus forming a colony of mature cells. (stemcell.com)
  • 4455512 ). Named colony-forming-unit fibroblasts (CFU-Fs) because of their spindle-like morphology, these cells showed nonhematopoietic differentiation potential. (miltenyibiotec.com)
  • After 7 days, cell count, hematopoietic stem cell-specific cluster of differentiation (CD) markers, colony-forming unit assay, and some microRNA gene expressions were assessed. (cancerindex.org)
  • Optimized culture media (MethoCult™) are available for CFU assays for the detection of erythroid (CFU-E, BFU-E), granulocyte/macrophage (CFU-G, CFU-M, CFU-GM) and multipotent progenitor cells (CFU-GEMM). (stemcell.com)
  • As shown in Figure 1 the combination of granulocyte-macrophage colony-stimulating factor (GM-CSF), stem cell factor (SCF) and interleukin-3 (IL-3) provides optimal growth of rat CFU-G, CFU-M and CFU-GM colonies from rat BM cells. (stemcell.com)
  • The optimal plating density for culture of rat CFU-GM colonies from BM cells is ~1 x 10 4 to 2 x 10 4 cells per 35 mm dish (Table 2). (stemcell.com)
  • Plating spleen and PB cells at two or even three different plating densities, e.g. 1 x 10 5 , 3 x 10 5 and 1 x 10 6 per 35 mm dish, is recommended to ensure that optimal colony numbers (50 - 100 per dish) are obtained with at least one of the plating densities. (stemcell.com)
  • The combination of GM-CSF, SCF and IL-3 provides optimal CFU-GM colony numbers from rat BM cells. (stemcell.com)
  • A complete erythropoietin (EPO)-containing methylcellulose medium, MethoCult™ SF M3436 (Catalog #03436, Table 1), originally developed for the culture of mouse burst-forming unit-erythroid (BFU-E) progenitors, is also suitable for colony assays of rat erythroid progenitor cells from BM, spleen and PB. (stemcell.com)
  • 1000 cells per colony) with a dense core, containing multiple erythroblast clusters (immature BFU-E, Figure 4B). (stemcell.com)
  • These colonies consisted of a single cluster (Figure 4C) or multiple clusters of cells that were larger and more distinct from each other than the erythroblasts in the BFU-E colonies and did not show evidence of hemoglobinization. (stemcell.com)
  • although the number of phenotypic hematopoietic stem cells (HSCs) in Setd2 -deleted mice is unchanged, functional assays, including serial BM transplantation, reveal that the self-renewal and competitiveness of HSCs are impaired. (nature.com)
  • The MSCs obtained from 5 different strains of mice were similar to human and rat MSCs in that they expanded more rapidly if plated at very low density, formed single-cell-derived colonies, and readily differentiated into either adipocytes, chondrocytes, or mineralizing cells. (bloodjournal.org)
  • The growth of mesenchymal stem cells was monitored by colony formation unit fibroblast (CFU-F) assays. (haematologica.org)
  • Results Multicolor cell sorting and CFU-F assays showed that mesenchymal stem cells were ~90-fold enriched in the MSCA-1 + CD56 − fraction and ~180-fold in the MSCA-1 + CD56 + fraction. (haematologica.org)
  • these cells grew to have the highest colony-forming unit (CFU) counts. (hindawi.com)
  • In order to broad- en the array of tools for cell-based autologous therapies, we iso- lated a novel renewable stem cell population from the adult testes that has characteristics of MSCs, termed gonadal stem cells (GSCs). (slideshare.net)
  • TrkA-mediated cell death was shown by the annexin-V binding assay to be, at least in part, apoptotic in both SK-N-MC and U2OS cells. (biomedsearch.com)
  • Furthermore, the truncated form (p18) of Bax accumulated in the TrkA-induced cells, suggesting that TrkA induces mitochondria-mediated apoptosis. (biomedsearch.com)
  • Moreover, MMP-9 is a stromal factor that regulates the mobilization of hemopoietic stem cells from the bone marrow niche by solubilizing the membrane-bound form of c-KitL ( 14 , 15 ). (aacrjournals.org)
  • Mice exposed in utero to low concentrations of benzene exhibit enduring changes in their colony forming hematopoietic cells. (cdc.gov)
  • Effects of low level maternal inhalation exposure to benzene (1076433) (Bz) on erythrocytic and granulocytic colony forming cells of fetal, neonatal, and young adult offspring were studied. (cdc.gov)
  • Assays of burst forming unit erythroid and colony forming unit erythroid (CFUE) cells were determined using a modified plasma clot method. (cdc.gov)
  • Granulocyte macrophage colony forming cells were assayed using lung media. (cdc.gov)
  • Exposure to 20ppm caused increases in the numbers of granulocytic macrophage colony forming cells among 2 day old neonates. (cdc.gov)
  • H460, SK-LU-1, and HBE cells formed larger aggregates in high-density cultures compared to low-density cultures. (nih.gov)
  • Indication, Collection, and Laboratory Processing of Cytologic Samples -- The Cellular and Acellular Components of the Urinary Sediment -- The Cytologic Makeup of the Urinary Sediment According to the Collection Technique -- Cytologic Manifestations of Benign Disorders Affecting Cells of the Lower Urinary Tract -- Tumors and Related Conditions of the Bladder and Lower Urinary Tract -- Urine-Based Assays Complementing Cytologic Examination in the Detection of Urothelial Neoplasm. (stanford.edu)
  • Intracellular survival assay was performed by infecting RAW264.7 cells with M. smegmatis before and after treatment with plant extracts. (biomedcentral.com)
  • In granulocyte colony-stimulating factor-mobilized autologous peripheral blood stem cells, the FISH ratio of interphase XYY cells to XY cells was 1.64, and there were 3.4 times more G-banded XYY metaphases than normal metaphases. (nih.gov)
  • QGR significantly increased the cell viability of BMNCs even in hypoxic condition, and mRNA expressions of stem cell factor, granulocyte macrophage-colony stimulating factor and erythropoietin from bone marrow stromal cells. (pharmalicensing.com)
  • It acts as an intracellular signal transducer in cells by cycling between the active, GTP-bound form and the inactive, GDP-bound form in response to diverse stimuli. (bloodjournal.org)
  • Studies performed in experimental and human diabetes have consistently shown an impaired mobilization of hematopoietic stem/progenitor cells (HSPC) from the bone marrow (BM) to peripheral blood (PB) after stimulation with granulocyte-colony stimulation factor (G-CSF) ( 1 ). (diabetesjournals.org)
  • A significant reduction was observed in colony forming efficiency, anchorage independence and growth rate of cells transfected with RNaseT2. (nih.gov)
  • B) A bar diagram showing average numbers of colonies for three independent experiments corresponding to cells and clones shown in part A. (C) Photograph of plates containing parent CSG2 cells, cells transfected with empty vector, negative transfer clone 5 and RNaseT2 expressing clones 3, 9 and 13. (nih.gov)
  • D) A bar diagram showing an average of colony counts of three independent experiments each for cells and clones shown in part C. (nih.gov)
  • 1.1 This test method covers a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments. (astm.org)
  • The readout of this assay is colorimetric, based on the reduction of XTT (a tetrazolium salt) by metabolically active fungal biofilm cells. (jove.com)
  • When parents donate UCB to a public cord blood bank (CBB) , the decision of whether or not to store the UCB unit is based primarily on the number of cells collected before and after processing. (parentsguidecordblood.org)
  • None of those units were characterized to ensure that the stem cells were of high-quality, let alone high potency. (parentsguidecordblood.org)
  • The second flawed assumption is that the stem cells in the UCB unit will exhibit the same quality and potency when they are thawed and prepared for a patient, as they did when the unit was collected and tested prior to cryopreservation. (parentsguidecordblood.org)
  • Reticular dysgenesis (RD) is the SCID form with the earlier clinical presentation (i.e., a few days after birth), due to the absence of both neutrophils and T cells. (haematologica.org)
  • Subsequent colony-forming unit (CFU) assays revealed that inhibition of mTORC1/2 suppresses the clonogenic function of hematopoietic progenitor cells (HPCs) in a dose-dependent manner. (sigmaaldrich.com)
  • Surprisingly, we found that dual inhibition of mTORC1/2 markedly inhibits the growth of day-14 cobblestone area-forming cells (CAFCs) but enhances the generation of day-35 CAFCs. (sigmaaldrich.com)
  • a unit of cells in bone marrow capable of generating or increasing the proliferation of new blood cells. (thefreedictionary.com)
  • For the study, published in the journal Heart Lung and Circulation, researchers used mouse models to investigate the impact of 1,25D -- a form of Vitamin D that interacts with hormones -- on the cardiac colony-forming unit fibroblasts (cCFU-Fs)cells that form scar tissue after a heart attack. (thefreedictionary.com)
  • Topics include a method to isolate and purify human bone marrow stromal stem cells, adipose-derived stem cells, colony-forming unit assays for MSCs, freezing and recovery of MSCs, and assays of MSCs with microrrays. (thefreedictionary.com)
  • Stromal cells are connective tissue cells that form the supportive structure in which the functional cells of the tissue reside. (wikipedia.org)
  • EpiCult-B media can assay for the presence of mammary precursor cells using either the mammary colony-forming cell (Ma-CFC) assay or 3D Matrigel-based cultures. (wikipedia.org)
  • Methylcellulose-based medium is widely used to detect and quantify hematopoietic progenitor cells in the colony-forming unit (CFU) assay. (wikipedia.org)
  • In a colonogenic assay, formation of burst forming unit-erythroid colony was enhanced by QGR treatment. (pharmalicensing.com)
  • In vivo, fertilized mammalian eggs undergo multiple cleavage divisions and form blastocysts (Fig. 1a ). (biomedcentral.com)
  • Bacterial membrane permeabilization was analyzed with the impermeable dye Sytox Green and mammalian membrane permeabilization with the hemolysis assay. (arvojournals.org)
  • However, contradictory evidences demonstrate that inhibition of osteoclast activity by bisphosphonate does not impair HSPC mobilization in response to Granulocyte-Colony Stimulating Factor (G-CSF) treatment [ 1 , 10 ], suggesting that osteoclasts may only intervene in certain types of hematopoietic stresses. (hindawi.com)
  • In diabetic mice, pioglitazone treatment downregulated Osm , p66Shc , and Cxcl12 in the hematopoietic BM, restored the effects of granulocyte-colony stimulation factor (G-CSF), and partially rescued HSPC mobilization, but it increased BM adipocytes. (diabetesjournals.org)
  • The viability of H. pylori inside macrophages was determined by using gentamicin colony-forming unit assay. (sigmaaldrich.com)
  • The colony-forming units assay and cell viability counts provided additional information to support the data from electron microscopy. (int-res.com)
  • Cell viability was analysed by apoptosis, proliferation and Colony Forming Unit assays. (cnio.es)
  • Cell viability was determined by adenosine triphosphate assay, and culture techniques were used to determine colony-forming unit (CFU) counts. (ovid.com)
  • The assay allows quantification of the ratio of a single bacterium within the biofilm, and its viable proportion. (springer.com)
  • They determined that the assay's detection limit was about 10 colony-forming units, or CFUs, per sample. (genomeweb.com)
  • As you may know, the D-10 value for a microorganism is defined as the UV dose necessary to affect a 90% reduction in colony-forming units (CFUs). (thefreedictionary.com)
  • In groups with positive growth, the Pearson linear correlation coefficient r (−1 ≤ r ≤ 1) between relative light units (RLUs) and log10 (CFUs + 1) was calculated. (ovid.com)
  • The results of the bioluminescence adenosine triphosphate assay and CFUs of the groups were analyzed by means of analysis of variance and the Duncan test. (ovid.com)
  • Variant specific discrepancy when quantitating BCR-ABL1 e13a2 and e14a2 transcripts using the Europe Against Cancer qPCR assay. (bioportfolio.com)
  • Molecular monitoring of treatment response in patients with chronic myelogenous leukemia is performed using the Europe Against Cancer (EAC) qPCR assay using the International Scale (IS). (bioportfolio.com)
  • SYBR Green and TaqMan-based qPCR assays were developed for quantifying Streptomyces scabies and S. lavendulae strains using primers targeting polymorphisms in the gamma hypervariable region of the 16S rRNA gene. (usda.gov)
  • The utility of qPCR TaqMan assay to accurately and rapidly quantify S. scabies and S. lavenduale strains inoculated together in complex soil communities is demonstrated. (usda.gov)
  • Midgut bacteria were quantified using colony forming unit (CFU) assays and real time quantitative polymerase chain reaction (RT-qPCR). (biomedcentral.com)
  • Representative examples of rat CFU-GM-derived colonies cultured in MethoCult™ GF R3774 are shown in Figure 2. (stemcell.com)
  • 90% of colonies cultured from rat BM in MethoCult™ SF M3436 were erythroid. (stemcell.com)
  • CFU assays are used to quantitate and characterize hematopoietic progenitors from tissues such as bone marrow (BM), spleen and peripheral blood (PB), and to investigate their responses to growth factors, inhibitors and drugs. (stemcell.com)
  • Quantifying Proliferative and Surface Marker Heterogeneity in Colony Founding Connective Tissue Progenitors and Their Progeny Using Time-Lapse Microscopy. (bioportfolio.com)
  • Colony-forming unit assays show suppression of lymphoid progenitors by each PAH within 6 h but a subsequent recovery, exclusively after BP treatment. (aspetjournals.org)
  • Concurrently, Cdc42 deficiency caused anemia and splenomegaly accompanied with decreased bone marrow erythroid burst-forming units (BFU-Es) and colony-forming units-erythroid (CFU-Es) activities and reduced immature erythroid progenitors, suggesting that Cdc42 deficiency causes a block in the early stage of erythropoiesis. (bloodjournal.org)
  • A small proportion of colonies were classified as non-erythroid (Figure 3). (stemcell.com)
  • The sensitivity of the assay was compared with colony forming units (CFU) counts. (springer.com)
  • The sensitivity of the assay was comparable to that of the CFU counts. (springer.com)
  • Hence TNC counts can be a misleading way to characterize high quality UCB units. (parentsguidecordblood.org)
  • Thereafter, we assessed the antimicrobial activity of exposed airway samples using both bioluminescence and standard colony forming unit assays. (ucsd.edu)
  • The isolated mMSCs from 5 inbred strains shared a number of characteristics, such as rapid growth from single-cell-derived colonies if plated at low densities. (bloodjournal.org)
  • Also, the non-cytotoxic effect of myrtenol on human peripheral blood mononuclear cell (PBMC) was evidenced by trypan blue and Alamar blue assays. (frontiersin.org)
  • Colony-Forming Cell Assay Detecting the Co-Expression of JAK2V617F and BCR-ABL1 in the Same Clone: A Case Report. (bioportfolio.com)
  • The Role of Nerve Growth Factor in Maintaining Proliferative Capacity, Colony-Forming Efficiency, and the Limbal Stem Cell Phenotype. (bioportfolio.com)
  • We also provide assay development and testing support for early phase clinical trials with a focus on cell and gene therapy. (cincinnatichildrens.org)
  • Genotoxicity was studied by DAPI staining and COMET assay using mouse macrophage RAW264.7 cell line. (biomedcentral.com)
  • The only cell functionality test presently used in this field, especially for umbilical cord blood transplantation, is the colony-forming unit (CFU) assay, which is subjective, non-validated and has been used since the early 1970s. (thefreedictionary.com)
  • The objective of this study is to determine whether the Air Barrier System device reduces airborne colony-forming units (e.g., bacteria) present at a surgery site during total shoulder art. (bioportfolio.com)
  • Alternate methods include bringing Gram positive bacteria into contact with the alkyl polyglycoside in other forms, e.g., when formulated with a pharmaceutically acceptable carrier or incorporated in or on a non-absorbent substrate. (google.com)
  • Transcriptional analysis unveiled the down-regulation of global regulator sarA and sarA mediated virulence genes upon myrtenol treatment, which is well correlated with results of phenotypic assays. (frontiersin.org)
  • 2. Term used in microbiology to designate a colony formed from a single bacterium or fungus. (thefreedictionary.com)
  • A colony forming unit assay was used to assess antimicrobial activity. (arvojournals.org)
  • In the study, published this month in the Journal of Clinical Microbiology , researchers from Great Basin, the University of North Carolina Hospitals, Indiana University Hospital, and the Medical College of Wisconsin tested the analytical sensitivity of the Great Basin C. diff assay by assaying different dilutions of cultured C. difficile spiked into a pooled negative stool sample at various concentrations. (genomeweb.com)
  • The GB assay correctly identified 31 of 32 positive samples, with one false negative and no false positives, yielding 97 percent sensitivity and 100 percent specificity. (genomeweb.com)
  • Effect of RNaseT2 overexpression on colony forming efficiency. (nih.gov)
  • That's why we have developed products specifically designed to automate colony counting and inhibition zone measurement, to relieve you of the tedium involved with these repetitive manual tasks. (synbiosis.com)
  • Great Basin's molecular testing platform consists of individual assay cartridges that run on an automated benchtop analyzer. (genomeweb.com)
  • Also, increased levels of microRNA-21 and microRNA-29a genes were observed in this group, while colony-forming ability was still maintained and high ranges of CD34(+), CD34(+)CD38(-), CD90(+), and CD117(+) phenotypes were observed as stemness signs. (cancerindex.org)
  • Enzyme extractions, solubilizations, and assays were performed as described (see Materials and Methods ). (pnas.org)
  • A separate sample of A-horizon soil was collected for microbial characterization by phospholipid fatty acid analysis (PLFA), soil enzyme assays, and determination of selected human and agricultural pathogens. (chemweb.com)
  • While colony forming unit enumeration has been the most commonly used method in the field, this technique has disadvantages and limitations, including slow growth of some fungal species on solid media and low and/or variable plating efficiencies, which is of particular concern when comparing growth of wild-type and mutant strains. (jove.com)
  • Colony-forming assays demonstrated the coexistence of 2 different haematopoietic clones: one was positive for the JAK2V617F mutation and the other co-expressed both JAK2V617F and the BCR-ABL1 fusion gene. (bioportfolio.com)
  • The TTDSL has effectively supported 11 Phase I/II gene therapy clinical trials by designing tailored analytical assays and acquiring sponsor-developed assays via technology transfer. (cincinnatichildrens.org)
  • In contrast, the T. cruzi Y strain neither induced AMP gene expression in the gut nor reduced the number of colony formation units in the anterior midgut. (biomedcentral.com)
  • For both strains the lower limit of detection with the TaqMan assay was 0.01 pg genomic DNA and the assay was linear to 10 ng input DNA. (usda.gov)
  • The lower detection limit for strains in native soil with the TaqMan assay was 102 colony-forming units/g soil. (usda.gov)
  • To investigate the mechanism by which CAPE enhanced HSPC homing, we performed these experiments including Q-PCR, western blot, immunohistochemistry and CFU assays after in-vivo HIF-1α activity blockade. (biomedcentral.com)
  • Using HSPC homing assays, we found that CAPE notably increased donor HSPC homing to recipient BM. (biomedcentral.com)
  • BM adipocytes contribute to forming the HSPC niche and their expansion after BM injury favors hematopoietic reconstitution ( 18 ). (diabetesjournals.org)
  • H460 and H1792 formed larger aggregates in polyHEMA cultures compared to methyl cellulose cultures. (nih.gov)
  • here we show that this forms a cellular antibacterial defense system. (elifesciences.org)
  • Antibacterial activity was determined by colony forming unit (CFU) assay. (biomedcentral.com)
  • Together these transcription factors form a key activator complex that regulates the expression of genes involved in leukemogenesis and its overexpression is significant related to adverse prognosis in AML. (cnio.es)
  • Transfer frequencies were expressed as the number of transconjugants per donor colony-forming unit after the mating period. (thefreedictionary.com)
  • This assay will be useful in understanding how different agricultural practices influence microbial populations that in turn affect crops and crop productivity. (usda.gov)
  • The platform is true sample-to-result, can perform on-demand testing instead of batching, multiplexes up to 64 distinct targets in a single assay, and can provide test results in as little as 60 minutes, depending on the target. (genomeweb.com)
  • We produce a world leading range of automated colony counting and zone sizing products for the microbiology laboratory. (synbiosis.com)
  • At Synbiosis we are passionate about making colony counting and zone analysis easier. (synbiosis.com)