Increase of hematopoietic responses by triple or single helical conformer of an antitumor (1-->3)-beta-D-glucan preparation, Sonifilan, in cyclophosphamide-induced leukopenic mice. (1/3030)

It has been suggested that the immunopharmacological activity of soluble (1-->3)-beta-D-glucan depends on its conformation in mice. In this study, we examined the relationship between the conformation of Sonifilan (SPG) and hematopietic responses in cyclophosphamide (Cy)-induced leukopenic mice. SPG, a high molecular weight (1-->3)-beta-D-glucan, has a triple helical conformation in water, and it was changed by treatment with aqueous sodium hydroxide to the single helical conformer (SPG-OH). The effects of SPG or SPG-OH on hematopoietic responses in cyclophosphamide induced leukopenic mice were investigated by monitoring i) gene expression of cytokines by RT-PCR, ii) protein synthesis of interleukin 6 (IL-6) by ELISA and iii) colony formation of bone marrow cells (BMC). The mice administered Cy and SPG or SPG-OH expressed and produced higher levels of IL-6 mRNA and protein than the mice administered only Cy. Gene expression of NK1.1 was also induced by Cy/SPG (or SPG-OH) treatment. Induced gene expression of stem cell factor (SCF) and macrophage-colony stimulating factor (M-CSF) by SPG/SPG-OH were also found in in vitro culture of BMC from Cy treated mice. These results strongly suggested that conformation of the glucans, single and triple helix, are independent of the hematopietic response.  (+info)

Functional characterization of a novel hematopoietic stem cell and its place in the c-Kit maturation pathway in bone marrow cell development. (2/3030)

While the majority of purified pluripotential hematopoietic stem cells (PHSC) express c-Kit, the receptor for steel factor, we have phenotypically and functionally separated a distinct class of PHSC that does not express c-Kit. In contrast to c-Kit-positive (c-Kit(pos)) PHSC, the c-Kit-negative (c-Kit(neg)) PHSC do not proliferate in response to multiple hematopoietic growth factors in vitro and do not radioprotect or form macroscopic spleen colonies (CFU-s) when transplanted into lethally irradiated recipients. However, the c-Kit(neg) PHSC show delayed or slow reconstitution kinetics when cotransplanted with radioprotective bone marrow cells. c-Kit(neg) PHSCs cells can give rise to c-Kit(pos) cells with CFU-s activity, radioprotective activity, and PHSC activity. Thus, constitutive hematopoiesis is maintained by c-Kit(pos) PHSCS cells that are recruited from a more primitive quiescent c-Kit(neg) PHSC population, which represents a critical developmental stage in definitive hematopoiesis.  (+info)

Single leukapheresis products collected from healthy donors after the administration of granulocyte colony-stimulating factor contain ten-fold higher numbers of long-term reconstituting hematopoietic progenitor cells than conventional bone marrow allografts. (3/3030)

Cytokine-mobilized peripheral blood progenitor cells (PBPCs) have been used successfully for hematopoietic reconstitution following allogeneic transplantation. The ease of harvest, the faster engraftment and the high yield of CD34+ cells have made this source of hematopoietic progenitor cells (HPCs) an attractive alternative to bone marrow (BM). In the present study we compared the engraftment potential of conventional BM allografts and single leukapheresis products (LPs) collected from healthy donors following the administration of granulocyte colony-stimulating factor (G-CSF). For this, lineage-committed and primitive HPCs were assessed by flow cytometry and by colony- and cobblestone area-forming cell (CFC, CAFC) assays. Mean numbers of CD34+ cells in LPs (n = 11) were similar to that of BM grafts (n = 12) (278+/-57 vs 227+/-34 x 10(6) CD34+ cells). The frequencies of CFCs, week 5 CAFCs and week 8 CAFCs were 1.6-, 8.4- and 10.3-fold higher in the CD34+ compartment of mobilized blood than that of marrow, resulting in significantly higher yields of clonogenic HPCs in LPs when compared to BM grafts. We conclude that G-CSF preferentially mobilizes clonogenic progenitors capable of short- and, in particular, longterm reconstitution, and that the engraftment potential of single LPs is superior to that of BM allografts. Hence, the use of PBPCs may be favorable for protocols that include graft manipulations with expected cell loss (eg T cell depletion, CD34+ selection). PBPCs may also be advantageous for gene therapy trials due to their high numbers of potential target cells (eg CAFCs).  (+info)

Influence of increased c-Myc expression on the growth characteristics of human melanoma. (4/3030)

Overexpression of the proto-oncogene c-myc has been associated with neoplastic transformation in a variety of tumors. For human melanoma high c-myc expression has been found in the vertical growth phase and higher positivity reported in metastases than primary tumors. The principle aim of this study was to determine, whether c-Myc expression influences the metastatic behavior of human melanoma in the absence of lymphocyte-mediated immune phenomena. The growth characteristics and tumor biology of two c-myc transfectants of the human melanoma cell line IGR39D, expressing c-Myc 1.7 and three times over baseline and the respective vector control were analyzed both in vitro and in a severe combined immunodeficient mouse model in vivo. Both c-myc transfectants showed increased growth rates, anchorage independent growth and directed cell movement in culture. Subcutaneously implanted IGR39D melanomas highly overexpressing c-Myc spontaneously formed macroscopic metastases (lymph nodes and lung) in severe combined immunodeficient mice in all cases (n = 7 per group), whereas less prominent c-Myc overexpression caused the development of only lung micrometastases. During the time period leading to terminal disease in animals injected with c-myc transfected human melanoma cells, melanoma development was not seen in vector controls. These findings suggest that constitutive high c-Myc expression in human melanoma results in a more aggressive growth behavior both in vitro and in vivo and favors metastasis in severe combined immunodeficient mice by factors unrelated to immune phenomena such as class I human leukocyte antigen downregulation known to be associated with c-Myc expression.  (+info)

Differential behaviors toward ultraviolet A and B radiation of fibroblasts and keratinocytes from normal and DNA-repair-deficient patients. (5/3030)

Xeroderma pigmentosum (XP) and trichothiodystrophy (TTD) are rare genodermatoses transmitted as recessive and autosomal traits that result in reduced capacity to repair UV-induced DNA lesions. Although XP, but not TTD, patients are prone to basal and squamous cell carcinomas, to date no comparative studies of the XP and TTD phenotypes have included epidermal keratinocytes. We compared the DNA repair capacity (by unscheduled DNA synthesis) and cell survival (by clonal analysis) of epidermal keratinocytes and dermal fibroblasts grown from normal individuals and patients with xeroderma pigmentosum and trichothiodystrophy following UVA and UVB irradiation. The same dose of UVB (1000 J/m2) induced twice as many DNA lesions in normal fibroblasts as in normal keratinocytes. UV survival rates were always higher in keratinocytes than in fibroblasts. Normal and TTD keratinocytes survived better following UVA and UVB irradiation than XP-C and XP-D keratinocytes. XP-C keratinocytes exhibited exacerbated sensitivity toward UVA radiation. Unscheduled DNA synthesis at UV doses leading to 50% cell survival indicated that the ratio of DNA repair capacity to cell survival is higher in keratinocytes than in fibroblasts. In addition, UVA and UVB irradiation induced a transition from proliferative to abortive keratinocyte colonies. This transition varied between donors and was in part correlated with their cancer susceptibility. Altogether these data provide the first evidence of the differential behaviors of normal, XP, and TTD keratinocytes toward UV radiation.  (+info)

Primitive hematopoietic progenitors within mobilized blood are spared by uncontrolled rate freezing. (6/3030)

Uncontrolled-rate freezing techniques represent an attractive alternative to controlled-rate cryopreservation procedures which are time-consuming and require high-level technical expertise. In this study, we report our experience using uncontrolled-rate cryopreservation and mechanical freezer storage at -140 degrees C. Twenty-eight PBPC samples (10 cryovials, 18 freezing bags) from 23 patients were cryopreserved in a cryoprotectant solution composed of phosphate-buffered saline (80%, v/v) supplemented with human serum albumin (10%, v/v) and dimethylsulfoxide (10%, v/v). The cryopreservation procedure required on average 1.5 h. The mean (+/- s.e.m.) storage time of cryovials and bags was 344+/-40 and 299+57 days, respectively. Although cell thawing was associated with a statistically significant reduction of the absolute number of nucleated cells (vials: 0.3x10(9) vs. 0.2x10(9), P< or =0.02; bags: 14x10(9) vs. 11x10(9), P< or =0.0003), the growth of committed progenitors was substantially unaffected by the freezing-thawing procedure, with mean recoveries of CFU-Mix, BFU-E, and CFU-GM ranging from 60+/- 29% to 134+/-15%. Mean recoveries of LTC-IC from cryovials and bags were 262+/-101% and 155+/-27% (P< or =0.2), respectively. In 14 out of 23 patients who underwent high-dose chemotherapy and PBPC reinfusion, the pre-and post-freezing absolute numbers of hematopoietic progenitors cryopreserved in bags were compared. A significant reduction was detected for CFU-Mix (11 vs. 7.4x10(5)), but no significant loss of BFU-E (180 vs. 150x10(5)), CFU-GM (400 vs. 290x10(5)) and LTC-IC (15 vs. 16x10(5)) could be demonstrated. When these patients were reinfused with uncontrolled-rate cryopreserved PBPC, the mean number of days to reach 1x10(9)/l white blood cells and 50x10(9)/l platelets were 9 and 13, respectively. In conclusion, the procedure described here is characterized by short execution time, allows a substantial recovery of primitive and committed progenitors and is associated with prompt hematopoietic recovery following myeloablative therapy even after long-term storage.  (+info)

Conclusions of a national multicenter intercomparative study of in vitro cultures of human hematopoietic progenitors. (7/3030)

With the aim of developing a standardized program of clonogenic cultures, a multicenter intercomparative study of human CFU-GM, BFU-E and CFU-GEMM cultures was conducted. Aliquots of fresh mononuclear cord blood cells, as well as uniform culture materials and instructions for cell culture and for colony scoring were distributed to 28 national laboratories involved in hematopoietic research and transplantation. High interlaboratory coefficients of variation (CV) in the reported number of progenitors were found in our first intercomparative study (range 67-231%). To investigate the relevance of colony scoring in variations of the reported colony numbers, participants were invited to attend a meeting where a single culture dish was scored. In this case, the CVs ranged from 31% to 81%. A subsequent intercomparative assay was then conducted, and significant reductions in the inter-laboratory CVs were obtained with respect to the first study (CVs for colonies grown with two different media: CFU-GMs, 48% and 55%; BFU-Es, 70% and 62%; CFU-GEMMs, 70% and 51%; respectively). In most instances CVs were not significantly different from those obtained in the single plate scoring study, suggesting that the scoring process was the most relevant parameter accounting for variations in the reported colony numbers.  (+info)

Role of interleukin 10 and transforming growth factor beta1 in the angiogenesis and metastasis of human prostate primary tumor lines from orthotopic implants in severe combined immunodeficiency mice. (8/3030)

Transfection of primary human prostate tumor cells (i.e., HPCA-10a, 10b, 10c, and 10d lines) with the transforming growth factor (TGF)-beta1 gene stimulated anchorage-independent growth and promoted tumor growth, angiogenesis, and metastasis after orthotopic implantation in severe combined immunodeficiency mice. In contrast, interleukin (IL)-10 transfected cells or cells cotransfected with these two genes exhibited reduced growth rates and significantly reduced angiogenesis and metastasis after 8, 12, and 16 weeks. Enzyme-linked immunosandwich assays confirmed that the respective tumors expressed elevated levels of TGF-beta1 and IL-10 in vivo. ELISAs further showed that TGF-beta1 expression induced matrix metalloproteinases-2 (MMP-2) expression, whereas IL-10 down-regulated MMP-2 expression while up regulating TIMP-1 in the transfected cells. Also, tumor factor VIII levels correlated with TGF-beta1 and MMP-2 expression and inversely with IL-10 and TIMP-1 levels. More importantly, mouse survival was zero after 4-6 months in mice bearing TGF-beta1- and MMP-2-expressing tumors and increased significantly in mice implanted with IL-10- and TIMP-1-expressing tumors (i.e., to >80% survival). Analysis of the metastatic lesions showed that they expressed TGF-beta1 and MMP-2 but barely detectable levels of IL-10 or TIMP-1, suggesting that IL-10 and TIMP-1 might normally block tumor growth, angiogenesis, and metastasis.  (+info)

*Stemcell Technologies

EpiCult-B media can assay for the presence of mammary precursor cells using either the mammary colony-forming cell (Ma-CFC) ... Methylcellulose-based medium is widely used to detect and quantify hematopoietic progenitor cells in the colony-forming unit ( ... and computer system designed specifically for automated imaging and counting of hematopoietic colonies in colony-forming unit ( ... STEMCELL Technologies Media for Hematopoietic Colony Assays MegaCult™ - Detect Megakaryocyte Progenitor Cells , STEMCELL ...

*Mesenchymal stem cell

In this assay system, stromal cells were referred to as colony-forming unit-fibroblasts (CFU-f). The first clinical trials of ... The majority of modern culture techniques still take a colony-forming unit-fibroblasts (CFU-F) approach, where raw unpurified ... Siminovitch L, Mcculloch EA, Till JE (1963). "The distribution of colony-forming cells among spleen colonies". Journal of ... "Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method". ...

*Colony-forming unit

"Optimized digital counting colonies of clonogenic assays using ImageJ software and customized macros: comparison with manual ... In microbiology, a colony-forming unit (CFU, cfu, Cfu) is a unit used to estimate the number of viable bacteria or fungal cells ... Instead of colony-forming units, the parameters Most Probable Number (MPN) and Modified Fishman Units (MFU)[citation needed] ... Expressing results as colony-forming units reflects this uncertainty. The purpose of plate counting is to estimate the number ...

*CFU-E

... colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue ... CFU-E stands for Colony Forming Unit-Hematopoietic . It arises from CFU-GEMM (via BFU-E, which stands for "erythroid burst- ... forming units") and gives rise to proerythroblasts. Understanding the murine CFU-e assay (analogous to human assay): CFU-e is a ... Marley SB, Lewis JL, Goldman JM, Gordon MY (June 1996). "Abnormal kinetics of colony formation by erythroid burst-forming units ...

*Titanocene Y

"Antiproliferative activity of Titanocene Y against tumor colony-forming units". Anticancer Drugs. 18 (3): 317-321. doi:10.1097/ ... "Analyses of Titanocenes in the spheroid-based cellular angiogenesis assay". Toxicol In Vitro. 22 (2): 531-534. doi:10.1016/j. ... Titanocene Y can be given in the mouse in high dosages and it shows generally mild toxicity in the form of diarrhea. Titanocene ...

*Clostridium butyricum

106 colony forming units (CFU) of C. butyricum MIYAIRI 588 (as active agent). CBM 588 does not establish permanently in the gut ... has been demonstrated by PCR assay. ... Clostridium butyricum is a strictly anaerobic endospore-forming ...

*Cell counting

... it can be generally assumed that each cell will give rise to a single colony or Colony Forming Unit (CFU). The colonies can ... for instance the Luria-Delbrück experiment or the gentamicin protection assay). In addition, the enumeration of colonies on ... instead of obtaining single colonies that can be counted, a so-called "lawn" will form: thousands of colonies lying over each ... Additionally, plating is the slowest method of all: most microorganisms need at least 12 hours to form visible colonies. ...

*Stem cell

Siminovitch L, Mcculloch EA, Till JE (1963). "The distribution of colony-forming cells among spleen colonies". Journal of ... "Precursors for fibroblasts in different populations of hematopoietic cells as detected by the in vitro colony assay method". ... "Neurons derived from radial glial cells establish radial units in neocortex". Nature. 409 (6821): 714-20. doi:10.1038/35055553 ... Some stem cells form tumors after transplantation; pluripotency is linked to tumor formation especially in embryonic stem cells ...

*Indoor bioaerosol

... in which colony forming units (CFU) on selective media are counted. Cultivating methods have several disadvantages. Culture- ... enzyme-linked immunosorbent assay (ELISA)). The well-known PCR is a powerful tool in identifying and even quantifying the ... This underestimation is likely to be signified for the quantification of bioaerosol, since colony counts of airborne microbes ... irrelevance of sampling units to human exposure measurement; multiplicity and variability of composition, etc.). To enable ...

*List of Legionnaires' disease outbreaks

This group has published guidelines about the actions to be taken to limit the number of colony forming units (i.e., the " ... disease by serological assays". BMC Infectious Diseases. 15: 163. doi:10.1186/s12879-015-0903-2. PMC 4383209 . PMID 25887275. ... The tabled figures are for total aerobic plate count, cfu/ml at 30 °C (minimum 48 hours incubation) with colony count ... of freshwater sites by PCR hybridization assay.[citation needed] Legionella bacteria themselves can be inactivated by UV light ...

*Acinetobacter

The other reliable identification test at genus level is chromosomal DNA transformation assay. In this assay, a naturally ... Acinetobacter is frequently isolated in nosocomial infections, and is especially prevalent in intensive care units, where both ... In healthy individuals, Acinetobacter colonies on the skin correlate with low incidence of allergies; Acinetobacter is thought ... Gene-silencing antisense oligomers in a form called peptide-conjugated phosphorodiamidate morpholino oligomers have also been ...

*Bacteriological water analysis

The unit of measurement is cfu/ml (or colony forming units per millilitre) and relates to the original sample. Calculation of ... samples contain a variety of components that can interfere with the ATP assay. The plate count method relies on bacteria ... Colonies that develop in the body of the medium can be counted by eye after incubation. The total number of colonies is ... growing a colony on a nutrient medium so that the colony becomes visible to the naked eye and the number of colonies on a plate ...

*American Silver Eagle

This time his legislation took the form of an amendment (S.AMDT.418) to H.R. 47, the "Statue of Liberty-Ellis Island ... Secretary of the Treasury James A. Baker III presided over the striking ceremony held at the San Francisco Assay Office. ... The sets sold out at the issue limit of 75,000 units. In fewer than twenty known sets, the Sacagawea Dollars do not have a ... above the eagle are thirteen five-pointed stars representing the Thirteen Colonies. The reverse is inscribed with the phrases ...

*Bacteriophage T12

Titers of plaques can be found by diluting the samples and counting plaque-forming units (PFUs). Biochemical tests such as ... Plaque assays consist of pouring a soft agar solution with an indicator strain onto an agar plate. The indicator strain should ... In addition, none of the colonies containing the T12 genome was negative for speA, and therefore, the conclusion was drawn that ... "Plaque Assay Protocols". Microbe Library. American Society for Microbiology. Archived from the original on 30 November 2012. ...

*History of Boston

The competing Plymouth Colony had been founded in 1620; it would later merge with the Massachusetts Bay Colony in 1691. In June ... There were 1,502 units in the development on 50 acres (200,000 m2) of land. In 1966, the Columbia Point Health Center opened ... Tremont Street still carries an alternate form of the original name. The two smaller peaks were Cotton Hill (named after John ... Town officials in colonial Boston were chosen annually; positions included selectman, assay master, culler of staves, fence ...

*DNA microarray

A form of replicate known as a dye flip can be performed to remove any dye effects in two-channel experiments; for a dye flip, ... Second, technical replicates (two RNA samples obtained from each experimental unit) help to ensure precision and allow for ... Microarray data is difficult to exchange due to the lack of standardization in platform fabrication, assay protocols, and ... and ant colony optimization. Input data for class prediction are usually based on filtered lists of genes which are predictive ...

*Beta-galactosidase

Each unit of β-galactosidase consists of five domains; domain 1 is a jelly-roll type barrel, domain 2 and 4 are fibronectin ... The β-galactosidase assay is used frequently in genetics, molecular biology, and other life sciences. An active enzyme may be ... The active ebg enzyme is an aggregate of ebgA -gene and ebgC-gene products in a 1:1 ratio with the active form of ebg enzymes ... After a time, certain colonies began to grow. However, the EbgA protein is an ineffective lactase and does not allow growth on ...

*Pound sterling

... the basic Roman unit of weight. The ISO 4217 currency code is GBP, formed from "GB", the ISO 3166-1 alpha-2 code for the United ... The original English colonies on mainland North America were not party to the sterling area because the above-mentioned silver ... Silverware made purely from melted coins would be found wanting when the silversmith took his wares to the Assay Office, thus ... The pound was a unit of account in Anglo-Saxon England, equal to 240 silver pennies and equivalent to one pound weight of ...

*Assay

Depending on the nature of the Detection system assays can be based on: Colony forming or virtual colony count: e.g. by ... number of assays done per unit time (usually expressed as per hour) etc. Organizations or laboratories that perform Assays for ... DNase footprinting assay Filter binding assay Gel shift assay Bicinchoninic acid assay (BCA assay) Bradford protein assay Lowry ... Chemotaxis assay Secretion assays Apoptosis assays such as the DNA laddering assay, the Nicoletti assay, caspase activity ...

*LOCAD

Endotoxin was found at every surface site where Colony Forming Units (CFUs) were observed with culture-based contact slide ... Implications for Immunosorbent Assay during Space Flight". J. Gravitational Physiology, Volume 10, Issue 2, 2004. J. Maule, M. ...

*Zinc finger chimera

1013 colony-forming units of phage. Following incubation, the phage are removed and the plate washed with buffer containing 0.5 ... These assays are repeated using different target oligonucleotides. When investigating zinc fingers binding 5'-XNN-3' sequences ... After elution, the phage can be plated and DNA extracted from individual plaque forming units, restricted and the appropriately ... This forms the basis of the investigation of ZFPs binding by phage display. Work is typically performed using the murine ZFP-TF ...

*Hematopoietic stem cell

Colony-forming unit-megakaryocyte (CFU-Meg) Colony-forming unit-basophil (CFU-B) Colony-forming unit-eosinophil (CFU-Eos) The ... A cobblestone area-forming cell (CAFC) assay is a cell culture-based empirical assay. When plated onto a confluent culture of ... Colony-forming unit-lymphocyte (CFU-L) Colony-forming unit-erythrocyte (CFU-E) Colony-forming unit-granulocyte-macrophage (CFU- ... which is a cell counting unit.) There are various kinds of HSC colony-forming units: Colony-forming unit-granulocyte- ...

*Staphylococcus haemolyticus

Detachment assays with NaIO4, proteinase K, or DNase result in 38%, 98%, and 100% detachment, respectively. The high level of ... The ability to adhere to medical devices and subsequently form biofilms is a major virulence factor associated with S. ... 2007). "Persistent strains of coagulase-negative staphylococci in a neonatal intensive care unit: virulence factors and ... 16 conventional growth tests including: colony pigment, DNase, alkaline phosphatase, ornithine decarboxylase, urease, acetoin ...

*Jewellery Quarter

To the northwest is Icknield Street which forms part of the A4540 Middle Ring Road. At the western apex of the area, the Middle ... The car park to the rear of the premises was created through the demolition of industrial units in the 1980s. On 6 May 2008, ... The proposals included an eight-storey flatted factory and 16 workshops with car parking above them as well as a new Assay ... Another gallery that was in the Jewellery Quarter was Colony, which opened in 2004 and closed in August 2008. There are also ...

*Oil shale

Britons of the Iron Age also used to polish it and form it into ornaments. The first patent for extracting oil from oil shale ... The hypothetical unit would see a cost reduction of 35-70% after producing its first 500 million barrels (79 million cubic ... On 2 May 1982, known in some circles as "Black Sunday", Exxon canceled its US$5 billion Colony Shale Oil Project near Parachute ... using the Fischer Assay. A 2016 estimate set the total world resources of oil shale equivalent to yield of 6.05 trillion ...

*Australian pound

The first coinage issued by the colony took place in 1813, and was effected by punching the middle out of Spanish dollars. This ... Australian dollar History of pound sterling in Oceania The Pound (in banknote form) was first issued in Australia in 1910 by a ... In 1852, the Government Assay Office in Adelaide issued gold pound coins. These weighed slightly more than sovereigns. From ... and Foreign Currency Units per 1 U.S. dollar, 1948-2005, PACIFIC Exchange Rate Service. Each source may contradict one another ...

*Gasoline

On the second day of battle, a unit of the XIX Corps was forced to halt when it ran out of gasoline.[39] One of the major ... Shortening gasoline to gas, which happens often, causes confusion with various forms of gaseous products also used as ... though this varies based on the crude oil assay. ... navy to protect the shipping of raw materials from its colonies ... However, before feeding those units, the naphtha needs to be split into light and heavy naphtha. Straight-run gasoline can be ...

*Taraxacum officinale

The leaves (called dandelion greens) can be eaten cooked or raw in various forms, such as in soup or salad. They are probably ... crops and its importance for colony development". Botany. 90 (7): 545-555. doi:10.1139/b2012-049.. ... Moreover, the diverse pharmacological activities of dandelion or individual compounds isolated thereof have only been assayed ... IU = International units. †Percentages are roughly approximated using US recommendations for adults. Source: USDA Nutrient ...
We studied the adhesion of primitive and committed progenitors from chronic myelogenous leukemia (CML) and normal bone marrow to stroma and to several extracellular matrix components. In contrast to benign primitive progenitors from CML or normal bone marrow, Ph1-positive primitive progenitors from CML bone marrow fail to adhere to normal stromal layers and to fibronectin and its proteolytic fragments, but do adhere to collagen type IV, an extracellular matrix component of basement membranes. Similarly, multilineage colony-forming unit (CFU-MIX) progenitors from CML bone marrow do not adhere to fibronectin or its adhesion promoting fragments but adhere to collagen type IV. Unlike committed progenitors from normal bone marrow, CML single-lineage burst-forming units-erythroid and granulocyte/macrophage colony-forming units fail to adhere to fibronectin or its components but do adhere to both collagen type IV and laminin. Evaluation of adhesion receptor expression demonstrates that fibronectin ...
Antiangiogenic therapy of cancer is highly attractive for several reasons (reviewed in Refs. 38, 39, 40 ). For instance, it can potentially overcome three major problems associated with other anticancer therapies, i.e., the problems of drug resistance (41 , 42) , poor delivery (43 , 44) , and tumor heterogeneity. One approach to the antiangiogenic therapy is antibody-based targeting of tumor vasculature. We have been targeting EDG on tumor vasculature in vivo (9 , 15 , 16) . Several features of EDG are described above (see "Introduction"). In addition, our anti-EDG mAbs showed little reactivity with normal human bone marrow cells (1) . This observation is consistent with our later finding that anti-EDG immunotoxin (i.e., ricin A-chain conjugate of SN6) selectively eradicated EDG-expressing cells without severely damaging normal human hematopoietic progenitors in the colony-forming unit assays.4 The results suggest that administration of therapeutic doses of an appropriate anti-EDG mAb and ...
Cell Cycle--drug effects;Cells, Cultured;Colony-Forming Units Assay;Hematopoietic Stem Cells--drug effects;Interleukin-3;Fluorouracil;Lymphokines--pharmacology;Mice;Spleen-- ...
Cell Cycle--drug effects;Cells, Cultured;Colony-Forming Units Assay;Hematopoietic Stem Cells--drug effects;Interleukin-3;Fluorouracil;Lymphokines--pharmacology;Mice;Spleen-- ...
We previously demonstrated that 3-azido-3-deoxythymidine (AZT) inhibits growth proliferation of human bone marrow progenitor cells in vitro [Antimicrob. Agents Chemother. 31:452-454 (1987)]. The present study evaluates the effect of toxic concentrations of AZT on possible sites of toxicity in human bone marrow cells. Exposure of cells over a 6-hr period to AZT concentrations between 0.5 and 50 microM resulted in a decreased incorporation of tritiated deoxyguanosine into DNA. Unchanged AZT and its phosphorylated metabolites accumulated within cells after exposure to 10 microM [3H]AZT. 3-Azido-3-deoxythymidine-5-monophosphate was the predominant metabolite, reaching a concentration of 49.2 +/- 14.1 pmol/10(6) cells after 48 hr, and a continuous increase was observed in all phosphorylated derivative levels between 2 and 48 hr of incubation. Using a highly sensitive and specific DNA polymerase assay, endogenous deoxyribonucleotide pool size(s) were analyzed for 48 hr after incubation of cells ...
Ten grams of hamburger were added to 90 mL of sterile buffer. This was mixed well in a blender. One-tenth of a mL of this slurry was added to 9.9 mL of sterile buffer. After thorough mixing, this suspension was further diluted by.
TY - JOUR. T1 - Endothelial colony-forming cell role in neoangiogenesis and tissue repair. AU - Critser, Paul J.. AU - Yoder, Mervin C.. PY - 2010/2/1. Y1 - 2010/2/1. N2 - Purpose of review: Patients suffering from vascular disease often have impaired angiogenic ability contributing to impaired tissue repair. One potential therapy is to deliver cells that can aid in angiogenesis. This review will discuss the ability of endothelial progenitor cells (EPCs), which have been reported to contribute to neoangiogenesis in both physiological and pathological conditions, to contribute to neoangiogenesis in tissue repair. Recent findings: In recent years, various reports have described conflicting roles for EPC in vessel formation. Currently there are three different assays for outgrowth of EPC all resulting in the isolation of different cell populations. This confusion is partially due to limited functional characterization of putative EPC populations. One population, endothelial colony-forming cell ...
BACKGROUND: Endothelial progenitor cells play a pivotal role in tissue repair, and thus are used for cell replacement therapies in "regenerative medicine." We tested whether the anesthetic sevoflurane would modulate growth or mobilization of these angiogenic cells. METHODS: In an in vitro model, mononuclear cells isolated from peripheral blood of healthy donors were preconditioned with sevoflurane (3 times 30 min at 2 vol% interspersed by 30 min of air). Colony-forming units were determined after 9 days in culture and compared with time-matched untreated control. Using magnetic cell sorting, CD133+/CD34+ endothelial progenitors were enriched from human umbilical cord blood, and vascular endothelial growth factor (VEGF), VEGFR2 (KDR), granulocyte colony-stimulating factor (G-CSF), STAT3, c-kit, and CXCR4 expressions were determined in sevoflurane-treated and untreated cells by real-time reverse transcriptase polymerase chain reaction. In a volunteer study with crossover design, we tested whether ...
Umbilical cord blood-derived endothelial colony-forming cells (UCB-ECFC) show utility in neovascularization, but their contribution to osteogenesis has not been defined. Cocultures of UCB-ECFC with human fetal-mesenchymal stem cells (hfMSC) resulted in earlier induction of alkaline phosphatase (ALP) (Day 7 vs. 10) and increased mineralization (1.9x; p < .001) compared to hfMSC monocultures. This effect was mediated through soluble factors in ECFC-conditioned media, leading to 1.8-2.2x higher ALP levels and a 1.4-1.5x increase in calcium deposition (p < .01) in a dose-dependent manner. Transcriptomic and protein array studies demonstrated high basal levels of osteogenic (BMPs and TGF-βs) and angiogenic (VEGF and angiopoietins) regulators. Comparison of defined UCB and adult peripheral blood ECFC showed higher osteogenic and angiogenic gene expression in UCB-ECFC. Subcutaneous implantation of UCB-ECFC with hfMSC in immunodeficient mice resulted in the formation of chimeric human vessels, ...
Programmed cell death-2 (PDCD2) protein is enriched in embryonic, hematopoietic, and neural stem cells, however, its function in stem/progenitor cell differentiation is unclear. We investigated the effects of PDCD2 knockdown on the development and differentiation of hematopoietic progenitor cells (HPC). CD34(+) cells derived from normal human bone marrow and K562 leukemic cells were effectively transduced with short-hairpin RNA to knockdown PDCD2. Colony-forming assays were used to investigate the effects of PDCD2 loss on HPC clonogenic potential and on 12-O-tetradecanoyl-phorbol-13-acetate-and arabinofuranosylcytosine-induced terminal differentiation. In CD34(+) clonogenic progenitors, PDCD2 knockdown decreased the total number of colony-forming units, increased the number of colony-forming units-granulocyte-erythroid-macrophage-megakaryocyte and burst-forming unit-erythroid primitive colonies, and decreased the number of burst-forming unit-erythroid mature colonies. Similar results were ...
To expand on the findings from previous clinical trials that the growth of residual tumor is increased at a predictable time following initial drug administration, malignant plasma cells from bone marrows of patients with multiple myeloma (MM) were examined for changes in proliferation and clonogenicity induced in vivo by cyclophosphamide and in vitro by drug-induced humoral stimulatory activity. Peak plasma cell [3H]thymidine labeling index (LI) occurred predictably following drug and paralleled changes in agar colony formation by marrow cells obtained during therapy. Colony-forming capacity of pretreatment MM marrow populations was enhanced when those cells were cultured with humoral stimulatory activity, similar to the increased colony formation detected in Day 9 postcyclophosphamide marrows at the time of peak plasma cell LI.. To further define a relationship between proliferative plasma cells and colony-forming tumor cells, MM Marrows were fractionated by sedimentation on an isokinetic ...
Lung Basal Stem Cells Rapidly Repair DNA Damage Using the Error-Prone Nonhomologous End-Joining Pathway Investigators showed that human basal stem cells (BSCs) isolated from heavy smokers proliferate extensively, whereas their alveolar progenitor cell counterparts have limited colony-forming capacity. They demonstrated that this difference arises in part because of the ability of BSCs to repair their DNA more efficiently than alveolar cells following ionizing radiation or chemical-induced DNA damage. [PLoS Biol] Full Article , Press Release Differentiated State of Initiating Tumor Cells Is Key to Distinctive Immune Responses Seen in H-RasG12V-Induced Squamous Tumors In two doxycycline-inducible models where oncogenic H-RasG12V is targeted either to the epidermal basal/stem cell layer with a Keratin14-rtTA transgene, or committed progenitor/suprabasal cells with an Involucrin-tTA transgene, investigators observed strikingly distinct tumor immune responses. These data showed that position of tumor ...
Effects of 1R-Chl on colony formation of CML patient MNCs and normal human MNCs.(A) MNCs from CML patients were cultured with rh stem cell factor, rh IL-3, rh G
Vascular Incorporation of Endothelial Colony-Forming Cells Is Essential for Functional Recovery of Murine Ischemic Tissue following Cell Therapy
SmartDish™ is meniscus-free cultureware that improves the accuracy of manual and automated counting of hematopoietic colony assays.
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Abstract. We show here for the first time that pluripotent hematopoietic stem cells express the CD4 antigen. CD4+ cells isolated from mouse marrow repopulated
TY - JOUR. T1 - Intrinsic Hematopoietic Stem Cell/Progenitor Plasticity. T2 - Inversions. AU - Colvin, Gerald A.. AU - Lambert, Jean François. AU - Moore, Brian E.. AU - Carlson, Jane E.. AU - Dooner, Mark S.. AU - Abedi, Mehrdad. AU - Cerny, Jan. AU - Quesenberry, Peter J.. PY - 2004/4. Y1 - 2004/4. N2 - Traditional concepts indicate that stem cells give rise to progenitor cells in a hierarchical system. We studied murine engraftable stem cells (ESCs) and progenitors in in vitro and found that ESC and progenitors exist in a reversible continuum, rather then a hierarchy. B6.SJL and BALB/c marrow cells were serially cultured with thrombopoietin (TPO), FLT-3 ligand (FLT-3L), and steel factor through cell cycle. Progenitors (high-proliferative potential colony-forming cells (HPP-CFC) and colony-forming unit culture (CFU-c)) and ESC capacity was determined. The cell cycle status of purified lineage negativerhodaminelowHoechstlow stem cells was determined under the same conditions using tritiated ...
L-selectin acts as a "homing receptor" for lymphocytes to enter secondary lymphoid tissues via high endothelial venules. Ligands present on endothelial cells will bind to lymphocytes expressing L-selectin, slowing lymphocyte trafficking through the blood, and facilitating entry into a secondary lymphoid organ at that point.[2] The receptor is commonly found on the cell surfaces of T cells. Naive T-lymphocytes, which have not yet encountered their specific antigen, need to enter secondary lymph nodes to encounter their antigen. Central memory T-lymphocytes, which have encountered antigen, express L-selectin to localize in secondary lymphoid organs. Here they reside ready to proliferate upon re-encountering antigen. Effector memory T-lymphocytes do not express L-selectin, as they circulate in the periphery and have immediate effector functions upon encountering antigen. High expression of L-selectin on human bone marrow progenitor cells is an early sign of cells becoming committed to lymphoid ...
A methodology for selection of the CD8 cell subset from the peripheral blood and bone marrow mononuclear cells was developed using anti-T8 (CD8) antibody and magnetic microspheres coated with anti-mouse IgG. Following optimization of antibody:cell binding ratio and microsphere:cell ratios, CD8(+)-cells in the peripheral blood and bone marrow were effectively removed, with an overall final recovery of 34.9% +/- 8.6%, and 56% +/- 8.5% respectively with complete recovery of stem cells and very little contamination with effector cells. CD8(+)-depleted cell preparations demonstrated a 3-4 fold increase in CFU-C and CFU-E colony formation over non-depleted preparations when stimulated with G-CSF, GM-CSF or IL-3 and erythropoietin. The largest increase in colony formation was evident when IL-3 was used to stimulate colony formation. Purified autologous CD8+ T-cells or culture supernatant from in vitro cultures of purified autologous CD8+ T-cells added back to CD8 depleted preparations, induced 20%-90%
Hemopoietic precursors with the ability to differentiate into wide varieties of cell types are considered primitive, as are precursors with long-term repopulating ability. Here we study the populations of marrow precursors from which both myeloid and lymphoid lineages are descended shortly after transplantation. Surprisingly, few or none of these precursors show long-term repopulating ability. Equal portions of a mixture of marrow cells from C57BL/6J (B6) and congenic B6-Hbbd Gpi-1a mice are transplanted into a group of recipients. Three weeks later, highly significant correlations between percentages of B6 type T cells, B cells, granulocytes, and platelets in each recipient indicate that many lymphoid and myeloid cells are descended from common precursors. After 4-6 weeks, most correlations between lymphoid and myeloid cells improve, indicating that most or all differentiated cells are descended from common precursors. The more differentiated myeloid-specific precursors found in spleen
Differentiation of Neural-Crest-Derived Intermediate Pluripotent Progenitors into Committed Periodontal Populations Involves Unique Molecular Signature Changes, Cohort Shifts, and Epigenetic Modifications. Smit Jayant Dangaria, Yoshihiro Ito, Xianghong Luan, Thomas G.H. Diekwisch. Stem Cells Dev. 2011 January; 20(1): 39-52. Published online 2010 July 6. doi: 10.1089/scd.2010.0180. PMCID: PMC3128775 ...
PRIME-XV® Mouse Hematopoietic Cell Basal Medium was developed in a collaboration program with a leading stem-cell research facility in the USA to fulfill the need for a serum-free, cost-effective medium for in vitro culture and expansion and self-renewal of murine hematopoietic progenitor cells (mHPCs).. PRIME-XV Mouse Hematopoietic Cell Basal Medium is:. ...
Confluent monolayers of LLC-MK2 cells used in FFU reduction assays were exposed to increasing concentrations of peptide before measuring selleck chemicals Tofacitinib mitochondrial reductase activity using an MTT mitochondrial reductase activity assay (Figure 3). When we initially performed these assays to exactly mimic the focus forming unit assay by waiting five days after peptide exposure, we saw no evidence of toxicity at any concentration of any peptide (data not shown). However, we found that a shorter post-exposure incubation time revealed a subtle toxicity on the part of one of the peptides. Apparently, waiting more than 24 h post-exposure gives the cells a chance to recover and conceals this effect. At 24 h post-exposure, DN57opt was found to be mildly toxic to cells at 40 ��M (one-way ANOVA with Dunnets post hoc test, P=0.. 0004, N=18), so only inhibitory data using lower, nontoxic concentrations was considered. Peptides DN57optscr, 1OAN1, and 1OAN1scr were not toxic at any ...
Stem cells are distinctive cells that can differentiate into any form of the cell of the body owing to its property of totipotency. Hence, they are a continuous resource of the specialized cells that built the organs and tissues.
Caltag Medsystems offer the following services: custom antibodies, angiogenesis services, drug discovery, stem cell assays, genotyping, custom MHC tetramers, flow cytometry, immunoassays, medical device testing and protein analysis.
Caltag Medsystems offer the following services: custom antibodies, angiogenesis services, drug discovery, stem cell assays, genotyping, custom MHC tetramers, flow cytometry, immunoassays, medical device testing and protein analysis.
Techniques for the development of ovine bone marrow-derived haemopoietic progenitor cells and in situ identification of colony morphology are described. Both mitogen stimulated lymphoid cells and antigen stimulated helper T-cells generated potent colony-stimulating activity in conditioned medium. Monocyte/macrophage, neutrophil, eosinophil, basophil/mast cell, neutrophil/monocyte and mixed phenotype colonies developed in stimulated bone marrow cultures in a conditioned medium dose-dependent manner. Neutrophil, monocyte/macrophage and eosinophil colonies were detected in greater numbers than the other types, with mixed colonies representing only around 1% of the total. Eosinophil colonies were particularly abundant when compared to published reports of the numbers obtained with similar cultures of normal mouse or human bone marrow cells. This culture technique will allow a detailed analysis of both ovine colony-stimulating factors and of the distribution of haemopoietic progenitor cells in vivo.
Cortical (cTEC) and medullary (mTEC) thymic epithelial cells establish key microenvironments for T-cell differentiation and arise from thymic epithelial cell progenitors (TEP). However, the nature of TEPs and the mechanism controlling their stemness in the postnatal thymus remain poorly defined. Using TEC clonogenic assays as a surrogate to survey TEP activity, we found that a fraction of cTECs generates specialized clonal-derived colonies, which contain cells with sustained colony-forming capacity (ClonoTECs). These ClonoTECs are EpCAM+MHCII-Foxn1lo cells that lack traits of mature cTECs or mTECs but co-express stem-cell markers, including CD24 and Sca-1. Supportive of their progenitor identity, ClonoTECs reintegrate within native thymic microenvironments and generate cTECs or mTECs in vivo. Strikingly, the frequency of cTECs with the potential to generate ClonoTECs wanes between the postnatal and young adult immunocompetent thymus, but it is sustained in alymphoid Rag2-/-Il2rg-/- counterparts. ...
Hotair is a member of the recently described class of noncoding RNAs called lincRNA (large intergenic noncoding RNA). Various studies suggest that Hotair acts regulating epigenetic states by recruiting chromatin-modifying complexes to specific target sequences that ultimately leads to suppression of several genes. Although Hotair has been associated with metastasis and poor prognosis in different tumor types, a deep characterization of its functions in cancer is still needed. Here, we investigated the role of Hotair in the scenario of epithelial-to-mesenchymal transition (EMT) and in the arising and maintenance of cancer stem cells (CSCs). We found that treatment with TGF-1 resulted in increased Hotair expression and triggered the EMT program. Interestingly, ablation of Hotair expression by siRNA prevented the EMT program stimulated by TGF-1, and also the colony-forming capacity of colon and breast cancer cells. Furthermore, we observed that the colon CSC subpopulation (CD133(+)/CD44(+)) ...
Stem Cell Assay Market By Assay (Cell Viability, Cell Differentiation, Cell Identification), By Technology, By Kit (Adult Stem Cell Kits, Human Embryonic Stem Cells Kits), By Product (Instruments, Detection Kits), By Application (Regenerative Medicines, Drug Development And Clinical Research), By End User (Research Institutes And Industry Research) And By Region - Global Industry Analysis, Size, Share, Growth, Trends, And Forecasts (2016-2021)
Evaluating the function of an individual hematopoietic stem cell (HSC) is a difficult and important problem. The functional ability per HSC, as well as the HSC concentration, was measured with minimal disruption to the cells in vivo using the new competitive dilution assay. Distribution of HSC into recipients was modeled based on Poisson probabilities. Predictions of donor contributions from models assuming different levels of donor HSC functional ability and concentration were compared to actual observations. The model with the least difference between predictions and observations was accepted. In BALB/ cBy (BALB) mice, models assuming equal functional ability of HSC from the same donor fit extremely well with actual observations, suggesting that all HSC are functionally homogeneous at any particular time point during development or aging. Relative HSC functional ability per cell declined during development, so that a fetal HSC had 1.6 to 3.0 times the functional ability of a young adult
AABB Hematopoietic Progenitor Cell (HPC) activities include educational programs, publications and accreditation for HPC programs.. The list of AABB Accredited HPC Facilities specifies those HPC facilities, in the US and throughout the world, which have attained AABB accreditation. These facilities are responsible for procuring, processing and storing hematopoietic progenitor cells that can be used for transplantation.. ...
Zeisberger, S. M., Zoller, S., Riegel, M., Chen, S., Krenning, G., Harmsen, M. C., ... Zisch, A. H. (2010). Optimization of the culturing conditions of human umbilical cord blood-derived endothelial colony-forming cells under xeno-free conditions applying a transcriptomic approach. Genes to cells, 15(7), 671-687. https://doi.org/10.1111/j.1365-2443.2010.01409.x ...
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The balance between endothelial injury and repair in childhood is poorly understood. We examined this relationship in healthy children, in adults, and in children with familial hypercholesterolemia (FH). Circulating endothelial cells (CECs) wer
Effect of GPC3 on cell proliferation and clonogenic capacity of liver CD90+GPC3+CSCs.(A) Cell proliferation was assessed after GPC3 knockdown in PLC CD90+GPC3+
RATIONALE : Laboratory-treated T cells may stimulate the immune system in different ways and stop tumor cells from growing. Drugs used in chemotherapy,
... allows one to test the capability of adherent cells to survive and replicate following insult with chemicals or radiation. Count the crystal violet stained colonies which incubated for 9 days with appropriate chemical or radiation dose and calculate the survival rate. - Clonogenic Assay - AbVideo™ - Support - Abnova
Chitra Venugopal is the author of this article in the Journal of Visualized Experiments: Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
The second problem with antibiotics is that they interfere with detection. QC Microbiology tells us their tests are sensitive to 1 colony-forming unit (CFU) in the sample, which means if there is 1 CFU in a 40mL sample bottle, theyll find it. But 1 CFU/40mL is 25 CFU/L... and for a 12,000L bioreactor, you need a contamination of ~300,000 CFUs in order for QC to detect contamination ...
thymodepressin: a synthetic peptide, promoted take of transplanted hemopoietic precursor cells in the bone marrow of irradiated mice
TY - JOUR. T1 - CD34 expression on long-term repopulating hematopoietic stem cells changes during developmental stages. AU - Matsuoka, Sahoko. AU - Ebihara, Yasuhiro. AU - Xu, Ming Jiang. AU - Ishii, Takefumi. AU - Sugiyama, Daisuke. AU - Yoshino, Hiroshi. AU - Ueda, Takahiro. AU - Manabe, Atsushi. AU - Tanaka, Ryuhei. AU - Ikeda, Yasuo. AU - Nakahata, Tatsutoshi. AU - Tsuji, Kohichiro. PY - 2001/1/15. Y1 - 2001/1/15. N2 - The CD34 antigen serves as an important marker for primitive hematopoietic cells in therapeutic transplantation of hematopoietic stem cells (HSC) and gene therapy, but it has remained an open question as to whether or not most HSC express CD34. Using a competitive long-term reconstitution assay, the results of this study confirm developmental changes in CD34 expression on murine HSC. In fetuses and neonates, CD34 was expressed on Lin-c-Kit+ long-term repopulating HSC of bone marrow (BM), liver, and spleen. However, CD34 expression on HSC decreased with aging, and in mice older ...
Fig. 4 Functional assays show increased transformation potential and sensitivity to TNK2 inhibition.. (A) Total colony formation in mouse bone marrow colony formation assay. Mouse bone marrow cells were cotransduced to express PTPN11, PTPN11 E76K, TNK2, or empty vector controls. Cells were selected for GFP+ (green fluorescent protein-positive) and puromycin resistance and plated in a methylcellulose GM-CSF sensitivity colony formation assay. Colonies were counted at 14 days [GM-CSF] = 0.05 nM (0.71 ng/ml). ****P , 0.0001 by one-way ANOVA. (B) Total colony formation in mouse bone marrow colony formation assay in cells transduced with PTPN11, PTPN11 E76K, or PTPN11 G60R. Cells were sorted for GFP+. Cells were plated with increasing concentrations of dasatinib. ***P , 0.005 and ****P , 0.0005 by one-way ANOVA. (C) Total colony formation and percent total colony formation in mouse bone marrow colony formation assay. Mouse bone marrow cells were cotransduced to express PTPN11 E76K and TNK2 or TNK2 ...
Cord Blood-CD36+ Early Erythroid Progenitor cells are derived from Cord Blood-CD34+ cells in culture. Cord Blood-CD34+ cells are cultured for seven to eight days using StemSpan SFEM (StemCell Technologies, Inc.), a serum free expansion media for hematopoietic cells, EPO (3U/ml), SCF (50ng/ml), IL-3 (10ng/ml), and IL-6 (10ng/ml) growth factors. The cells are harvested from culture, and the Cord Blood-CD36+ cells are positively isolated using a direct immunomagnetic CD36 MicroBead labeling system ...
Figure 1A shows the results of a typical survival study.19 A single subcutaneous (sc) injection of DT3 (400 mg/kg) 24 h before TBI (8.75 Gy, Cobalt 60 γ-radiation, 0.6 Gy/min) protected CD2F1 mice from radiation-induced mortality with 100% 30-day survival. In contrast, in control vehicle-injected mice, radiation-induced deaths started 8 days post-irradiation and the 30-day survival after TBI was only 18% (n=16). The time course for vehicle controls shown in Figure 1A is similar to that which we observed in other studies, and is consistent with mortality due largely to hematopoietic injury.19,21. We further determined the effects of DT3 on γ-irradiated mouse bone marrow hematopoietic progenitor and stem cell survival (Figure 1B). Bone marrow cells were collected from femora and humeri on days 1, 8 and 13 after irradiation and total live bone marrow myeloid cells for pooled samples from each mouse were measured by trypan blue staining. γ-irradiation caused mouse bone marrow cell death within 24 ...
BACKGROUND AND OBJECTIVE: We previously reported that patients with acquired severe aplastic anemia (SAA) treated with antilymphocyte globulin (ALG), 6-methylprednisolone, cyclosporin A (CyA) and granulocyte colony-stimulating factor (G-CSF) can mobilize peripheral blood hemopoietic progenitors (PBHP). The aim of the present study was to assess phenotypic and functional properties of these PBHP. METHODS: We studied seven patients who underwent 43 leukophereses (median 5) between day +30 and +80 following ALG, while in treatment with CyA and G-CSF. Mobilized peripheral blood hemopoietic progenitors were analyzed using surface markers, conventional assays for clonogenic cells (CFU-GM, BFU-E, CFU-GEMM) as well as the recently developed assay for long-term culture initiating cells (LTC-ICs). RESULTS: The proportion of CD34+ cells ranged between 0% and 5.4% (median 0.3%), CD34+DR between 0% and 3.5% (median 0.1%) and CD8+ cells between 3.3% and 56% (median 31%). When light density mononuclear cells ...
Minguell, J.J.; Bruzzone, M.S., 1986: Regulation of hydrocortisone binding sites by hydrocortisone in human bone marrow fibroblasts
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PIWIL proteins are stem cell self-renewal and maintenance associated proteins that bind Piwi interacting RNA (piRNA) to mediate epigenetic modifications in lower vertebrates. In solid cancers, PIWIL proteins have been described as putative proto-oncogenes. We could show that a human PIWI like protein-PIWIL4 is overexpressed in majority of AML patients, particularly in patients harboring the MLL-AF9 translocation. The knockdown of PIWIL4 in MLL-AF9 harboring cell lines and primary patient samples leads to a marked depreciation in growth in vitro and in vivo, and a global loss of H3K9me3 marks in cell lines. The loss of growth potential and decrease in global levels of H3K9me3 in cell lines could be rescued via overexpression of wild type PIWIL4, but not by a PIWIL4 mutant lacking the piRNA binding-PAZ domain. No growth inhibition or effect on colony formation was observed upon PIWIL4 depletion in normal human hematopoietic stem progenitor, in vitro. PIWIL4 depleted AML cell lines showed a deregulation of
This unit describes various sites from which human and murine hematopoietic stem cells (HSC) can be collected, including bone marrow, peripheral blood, and, in the case of human HSC, umbilical cord blood
Expression of Thy-1 on hematopoietic cells from human fetal liver (FL), cord blood (CB), and bone marrow (BM) was studied with a novel anti-Thy-1 antibody, 5E10. Specificity of 5E10 for human Thy-1 was demonstrated by immunoprecipitation of a 25-35-kD molecule, and the sequence of a cDNA that was cloned by immunoselection of COS cells transfected with a cDNA library derived from a 5E10+ cell line. Two- and three-color immunofluorescence staining experiments revealed that the Thy-1 expression is restricted to, an average, 1-4% of FL, CB, and BM cells, and binding to these cell types is essentially restricted to a very small subset of lymphoid cells and approximately 25% of CD34+ cells. Thy-1+ CD34+ cells were further characterized as CD38lo/CD45RO+/CD45RA-/CD71lo/c-kit(lo) and rhodamine 123dull. When CD34+ cells were sorted on the basis of Thy-1 expression, the majority of clonogenic cells were recovered in the CD34+Thy-1- fraction, whereas the majority of cells capable of producing myeloid ...
TY - JOUR. T1 - Properties of the mouse embryo conditioned medium factor(s) stimulationg colony formation by mouse bone marrow cells grown in vitro.. AU - Stanley, E. R.. AU - Bradley, T. R.. AU - Sumner, M. A.. PY - 1971/10. Y1 - 1971/10. UR - http://www.scopus.com/inward/record.url?scp=0015138971&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0015138971&partnerID=8YFLogxK. M3 - Article. C2 - 4333458. AN - SCOPUS:0015138971. VL - 78. SP - 301. EP - 317. JO - Journal of Cellular Physiology. JF - Journal of Cellular Physiology. SN - 0021-9541. IS - 2. ER - ...
Because of the ubiquitous expression of β-gal, ROSA26 mice might be useful to monitor engraftment of transplanted hematolymphoid cells, whether they are primitive stem/progenitor cell populations or mature end-stage cells. To this end, we performed several BM transplantations into lethally irradiated (750 rad; 1 rad = 0.1 Gy) recipient C57BL/6J mice, with either whole BM (2 × 106 cells) or cells partially enriched for hematopoietic stem/progenitor activity by sorting for cells that do not express antigens present on lineage-committed hematopoietic cells (1 × 105 Lin− cells). These cells were isolated from heterozygous ROSA26 mice backcrossed three generations to C57BL/6J and sorted to be CD5− Mac1− B220− CD4− CD8− Gr-1− (Lin−). β-gal expression in the hematolymphoid compartment of these mice showed that 4 weeks after transplantation, BM-derived progenitor cells could reconstitute all major hematolymphoid lineages as evidenced by the high proportion of β-gal+ cells found in ...
Detailed drug Information for hematopoietic progenitor cells, cord blood Intravenous. Includes common brand names, drug descriptions, warnings, side effects and dosing information.
Macrophages derived in vitro from bone marrow progenitor cells (BMDM) under the influence of CSF-1 or GM-CSF were compared for immune function. CSF-1- and GM-CSF-derived BMDM did not differ in their ability to kill L929 ...
Strating the activities of the host and sustaining homeostasis. The plasticity of hematopoietic progenitor cells in the BM bestows upon them the ultimate power
مخططات وطلاءات، ونطاقات الألوان، والتركيبات، والتدرجات، والفضاء اللوني، والتحويلات لألوان ‎#677cfe الست عشرية.
Acronyms and Abbreviations: AGM, aorta-gonad-mesonephros; BFU-E, burst-forming unit-erythroid; BFU-MK, burst-forming unit-megakaryocyte; CAFC, cobblestone area-forming cell; CAR, CXCL12-abundant reticular; CFC, colony-formingcell; CFU-E, colony-forming unit-erythroid; CFU-GM, colony-forming unit-granulocyte-macrophage; CFU-MK, colony-forming unit-megakaryocyte; CLP, common lymphoid progenitor; CMP, common myeloid progenitor; EBF, early B-cell factor; ECM, extracellular matrix; EGF, epidermal growth factor; EPO, erythropoietin; EPOR, erythropoietin receptor; FAK, focal adhesion kinase; FL, Flt-3 ligand; G-CSF, granulocyte colony-stimulating factor; G-CSF-R, granulocyte colony-stimulating factor receptor; GM-CSF, granulocyte-macrophage colony-stimulating factor; GM-CSF-R, granulocyte-monocyte colony-stimulating factor receptor; GMP, granulocyte-macrophage progenitor; HSC, hematopoietic stem cell; Ig, immunoglobulin; IL, interleukin; IRF4, interferon regulatory factor 4; LEF, lymphoid-enhancer ...
Since monocytes and macrophages that arise during the culture of bone marrow progenitor cells are potential sources of interleukin 6 (IL-6), we investigated whether auto- or paracrine production of this factor is involved in colony formation by normal hematopoietic progenitor cells. We added a polyclonal anti-IL-6 antiserum and a monoclonal anti-IL-6 antibody to cultures of monocyte- and T cell-depleted bone marrow cells. Colony formation was stimulated with granulocyte/monocyte-colony-stimulating factor (GM-CSF), monocyte-CSF, or IL-3. Addition of anti-IL-6 antibody resulted in decreased numbers of monocytic colonies to 40-50% of control values, whereas the numbers of granulocytic colonies were not altered. The inhibitory effect was preserved in cultures of CD34(+)-enriched bone marrow cells. As a second approach, we added a monoclonal antibody directed against the IL-6 receptor to cultures of monocyte- and T cell-depleted bone marrow cells. This antibody almost completely inhibited the growth ...
Looking for online definition of multipotential hemopoietic stem cell in the Medical Dictionary? multipotential hemopoietic stem cell explanation free. What is multipotential hemopoietic stem cell? Meaning of multipotential hemopoietic stem cell medical term. What does multipotential hemopoietic stem cell mean?
CFU-E stands for Colony Forming Unit-Hematopoietic . It arises from CFU-GEMM (via BFU-E, which stands for "erythroid burst-forming units") and gives rise to proerythroblasts. Understanding the murine CFU-e assay (analogous to human assay): CFU-e is a stage of erythroid development between the BFU-e stage and the pro-erythroblast stage. CFU-e colony assay is designed to detect how many colony-forming-units of erythroid lineage there are in a hematopoietic tissue (bone marrow, spleen, or fetal liver), which may be reflective of the organisms demand for oxygen delivery to the tissues or a hematopoietic disorder. Early erythroid progenitors are found at a quite low frequency relative to later stages of erythroid differentiation, such as the pro-erythroblast and the basophilic erythroblast stages which can be detected by flowcytometry directly ex-vivo (Socolovsky et al. 2001 PMID 11719363). Furthermore, unlike for the pro-erythroblast and later stages of erythroid development, no truly reliable and ...
We tested the effects of DP on erythroid and granulocyte-macrophage colony formation in progenitor cells from preterm infants and adults. The concentrations of DP included in the culture dishes ranged from 10−6 M to 10−9M. Studies on the pharmacokinetics of DP in preterm infants have shown that the plasma concentrations reached following DP administrations are in the order of magnitude of 10−6 M to 10−8M.7 It is not known, however, whether these concentrations will be achieved in the bone marrow, nor whether interactions with other hormones or mediators in the bone milieu will influence the effect of DP on haematopoietic progenitors. In our experimental conditions DP showed a dose dependent inhibitory effect on both erythroid and granulocyte-macrophage colonies derived from progenitor cells of preterm infants. CFU-E colonies were the most affected, with significant reductions observed at all concentrations of DP. This profound reduction in erythroid colonies was not apparent with bone ...
Holmes, T., Yan, F., Ko, K.-H., Nordon, R., Song, E., OBrien, T. A. and Dolnikov, A. (2012), Ex vivo expansion of cord blood progenitors impairs their short-term and long-term repopulating activity associated with transcriptional dysregulation of signalling networks. Cell Proliferation, 45: 266-278. doi: 10.1111/j.1365-2184.2012.00813.x ...
This is the first etiopathogenesis study of BNP which assesses the functionality of BM-HPCs. The results shown here demonstrate that BNP was induced in the challenge animals and that functional damage to the hematopoietic progenitor cells was apparent prior to the development of clinical signs, gross or histopathological lesions. As early as 24 hours after colostrum intake, the CFUs-GEMM were compromised in their colony forming ability and by day 6 the number of all CFU types was markedly reduced. This supports the hypothesis that the main target cell is the pluripotent hematopoietic progenitor cell. In addition, this study further demonstrates that the more differentiated cells (CFU-E and CFU-GM precursors) present in the bone marrow are apparently not compromised at 24 hours post-colostrum ingestion.. Lymphopenia post-colostral challenge has been previously observed [9, 10, 12]; therefore in this study we attempted to determine whether a specific subset of peripheral blood mononuclear cells ...
Circulating endothelial cells (CEC) are detached from the vessel wall endothelium as the result of injury and/or disease. Blood outgrowth endothelial cells (BOEC) have all the characteristics of mature endothelial cells. They seem to be progeny of endothelial colony-forming cells (ECFC), a marrow-derived progenitor that resides both in blood and within in situ endothelium. The other relevant cell appearing from appropriate culture of blood mononuclear cells was labeled "EPC" (intended for endothelial progenitor cells). These were later shown to be of hematopoietic stem cell (HSC) origin ...
Using a clonal culture system, we investigated the hemopoietic effects of purified recombinant IL-5 obtained from conditioned media of transfected Xenopus oocytes. IL-5 alone acted on untreated bone marrow cells and supported the formation of a small number of colonies, all of which were predominantly eosinophilic. However, it did not support colony formation by spleen cells from 5-FU-treated mice, in which only primitive stem cells had survived, while IL-3 and G-CSF did. Eosinophil-containing colonies were formed from these cells in the presence of IL-5 and G-CSF together. In contrast, G-CSF alone did not support any eosinophil colonies. The eosinophilopoietic effect of IL-5 was dose-dependent, and was neutralized specifically by anti-IL-5 antibody. To exclude the possibility of interactions with accessory cells in the same culture dish, we replated a small number (200 cells/dish) of enriched hemopoietic progenitors, obtained from blast cell colonies, which were formed by cultivation of spleen ...
Clone REA775 recognizes the human CD33 antigen, a 67 kDa glycoprotein belonging to the sialoadhesin superfamily. The CD33 antigen is highly expressed on human monocytes but weakly on granulocytes and some - but not all - myeloid dendritic cells. The CD33 antigen is also found on myeloid progenitor cells (CFU-GEMM, CFU GM, CFU-G, BFU-E) but is not expressed on lymphocytes, platelets, erythrocytes, or primitive hematopoietic stem cells. Additional information: Clone REA775 displays negligible binding to Fc receptors. - Singapore
Several recent studies have implicated the AhR as a constitutive repressor of hematopoietic stem cell differentiation (Singh et al., 2009b; Boitano et al., 2010). Thus, activation by TCDD suppresses hematopoiesis (Singh et al., 2009a). However, we show here that DMBA and BP activate AhR comparably with TCDD in BM but disrupt lymphoid and myeloid progenitors more rapidly, despite the need for bioactivation provided by Cyp1b1 metabolism. These two PAHs target lymphoid and myeloid progenitors similarly within 6 h. Paradoxically, BP selectively stimulates an AhR-mediated protection mechanism that also functions remarkably rapidly. These early effects on progenitor activities and their dependence on AhR and Cyp1b1 parallel the later decreases in immature lymphocytes and granulocytes that occur after 24 h (Fig. 3) (Heidel et al., 1998; Galván et al., 2006). The similar disruption effects on myeloid and lymphoid progenitors and their shared AhR-mediated protection each suggest that disruption may ...
TY - JOUR. T1 - Proliferation and maturation effects of in-vivo granulocyte-macrophage colony stimulating factor in acute non-lymphocyte luekaemia. AU - Damiani, D.. AU - Michieli, M.. AU - Revignas, M. G.. AU - Russo, D.. AU - Fanin, R.. AU - Michelutti, A.. AU - Mallardi, F.. AU - Baccarani, M.. PY - 1992. Y1 - 1992. UR - http://www.scopus.com/inward/record.url?scp=0026574360&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0026574360&partnerID=8YFLogxK. M3 - Article. C2 - 1398278. AN - SCOPUS:0026574360. VL - 77. SP - 25. EP - 29. JO - Haematologica. JF - Haematologica. SN - 0390-6078. IS - 1. ER - ...
Research proven mouse monoclonal CD34 antibody. Excellent marker for hematopoietic progenitors and stem cells. CD34 protein is involved in differentiating HPCs into certain types of neurons. Also useful for studying endothelial cells, angigogensis and tumorigenesis. Designed for immunohistochemisitry and related applications. IHC image available.
Sheila K. Singh is the author of this article in the Journal of Visualized Experiments: Processing of Primary Brain Tumor Tissue for Stem Cell Assays and Flow Sorting
A series of experiments published in the journal Nature Medicine suggest young adult women have primitive stem cells that could generate new eggs. The findings are generating both excitement and questions.
Rate your experience with methylcellulose 4000cps (bulk) on - RxList including the side effects, drug interactions, effectiveness, ease of use and satisfaction.
TY - JOUR. T1 - Runx1 expression marks long-term repopulating hematopoietic stem cells in the midgestation mouse embryo. AU - North, Trista E.. AU - De Bruijn, Marella F T R. AU - Stacy, Terryl. AU - Talebian, Laleh. AU - Lind, Evan. AU - Robin, Catherine. AU - Binder, Michael. AU - Dzierzak, Elaine. AU - Speck, Nancy A.. PY - 2002. Y1 - 2002. N2 - Hematopoietic stem cells (HSCs) are first found in the aorta-gonad-mesonephros region and vitelline and umbilical arteries of the midgestation mouse embryo. Runx1 (AML1), the DNA binding subunit of a core binding factor, is required for the emergence and/or subsequent function of HSCs. We show that all HSCs in the embryo express Runx1. Furthermore, HSCs in Runx1+/- embryos are heterogeneous and include CD45+ cells, endothelial cells, and mesenchymal cells. Comparison with wild-type embryos showed that the distribution of HSCs among these various cell populations is sensitive to Runx1 dosage. These data provide the first morphological description of ...
Recent developments of surrogate assays for human hematopoietic stem cells (HSC) have facilitated efforts at improving HSC gene transfer efficiency. Through the use of xenograft transplantation models, such as nonobese diabetic/severe combined immunodeficiency (NOD/SCID) mice, successful oncoretroviral gene transfer to transplantable hematopoietic cells has been achieved. However, because of the low frequency and/or homing efficiency of SCID repopulating cells (SRC) in bone marrow (BM), studies have primarily focused on cord blood (CB). The recently developed extended (| 60 days) long-term culture-initiating cell (ELTC-IC) assay detects an infrequent and highly quiescent candidate stem cell population in BM as well as CB of the CD34(+)CD38(-) phenotype. Although these characteristics suggest that ELTC-IC and SRC might be closely related, attempts to oncoretrovirally transduce ELTC-IC have been unsuccessful. Here, recently developed conditions (high concentrations of SCF + FL + Tpo in serum-free medium)
Hemopoietic stem cell factor (SCF), which is the ligand for the proto-oncogene c-kit receptor (allelic with W locus) and the product of Sl locus of the mouse, has recently been cloned. The human homologue has also been cloned, and recombinant protein (human rSCF) expressed and purified to homogeneity. To determine the effect of human rSCF in the presence or absence of human rIL-3 on human bone marrow-derived mast cells and basophils, human CD34+ pluripotent progenitor cells, highly enriched (greater than 99%) from bone marrow mononuclear cells, were cultured over agarose surfaces (interphase cultures) in the presence of human rIL-3, human rIL-3 and increasing concentrations of human rSCF, or human rSCF alone. Over 3 to 4 wk, human rSCF acted synergistically with human rIL-3 at all concentrations, producing a three- to fivefold increase in total, mast cell, and basophil numbers over human rIL-3 alone when used at 100 ng/ml. The percentage of cell types in the human rIL-3 and human rIL-3 plus ...
Conjugated Polymers Optically Regulate the Fate of Endothelial Colony-Forming Cells Scientists proposed a innovative strategy to gain optical control of endothelial colony-forming cell fate, which represented the only known truly endothelial precursor showing robust in vitro proliferation and overwhelming vessel formation in vivo. [Sci Adv] Full Article A Small-Molecule Pan-Id Antagonist Inhibits Pathologic Ocular Neovascularization The authors showed that the genetic loss of Id1/Id3 reduced ocular neovascularization in mouse models of wet age-related macular degeneration and retinopathy of prematurity. [Cell Rep] Full Article , Graphical Abstract Endothelial PKA Activity Regulates Angiogenesis by Limiting Autophagy through Phosphorylation of ATG16L1 Investigators showed that endothelial protein kinase A (PKA) activity mediates a critical switch from active sprouting to quiescence in part through phosphorylation of ATG16L1, which in turn reduced endothelial autophagy. [eLife] Full Article ...
CFU-GEMM is a colony forming unit that generates myeloid cells. CFU-GEMM cells are the multipotential progenitor cells for myeloid cells; they are thus also called common myeloid progenitor cells or myeloid stem cells. "GEMM" stands for granulocyte, erythrocyte, monocyte, megakaryocyte. The common myeloid progenitor (CMP) and the common lymphoid progenitor (CLP) are the first branch of cell differentiation in hematopoiesis after the hemocytoblast (hematopoietic stem cell). In current terminology, CFU-S refers to the pluripotent stem cells that can differentiate into all types of blood cells. CFU-S divides into two lineages: the lymphoid precursor (CFU-LSC) and the myeloid precursor (CFU-GEMM). The CFU-GEMM cell is capable of differentiating into white blood cells, red blood cells, and platelets, all of which are normally found in circulating blood. It has been suggested that eosinophils do not derive from the common myeloid progenitor in humans. In the adjacent image, CFU-GEMM is the scientific ...
Diabetes mellitus is associated with a significant reduction of circulating progenitor cells (CPCs). CPCs are defined by the surface expression of the stem cell antigen CD34 and or CD133. These cells include endothelial progenitor cells (EPCs), which are involved in cardiovascular homeostasis and repair. EPCs are characterized by the co-expression of endothelial antigen(s), such as KDR.. A reduction of CPCs in metabolic patients is associated with an increased risk of future adverse cardiovascular outcomes, such as myocardial infarction, stroke, revascularization, etc. Therefore, ways to active stimulate an increase of CPC levels in diabetes are actively pursued. Indeed, there are several drugs that stimulate CPCs or EPCs, but it is not fully clear if they are active also in diabetic patients.. The mechanisms that account for CPC reduction in diabetes include defective bone marrow mobilization, reduced survival and increased homing outside the bloodstream. Experimental animal studies and ...
In refractory anemia (RA) and refractory anemia with ringed sideroblasts (RARS) a discrepancy is observed between the decreased in vitro erythroid colony formation and the normal or increased number of normoblasts in the bone marrow. To study the in
Looking for online definition of Pluripotential hemopoeitic stem cell in the Medical Dictionary? Pluripotential hemopoeitic stem cell explanation free. What is Pluripotential hemopoeitic stem cell? Meaning of Pluripotential hemopoeitic stem cell medical term. What does Pluripotential hemopoeitic stem cell mean?
TY - JOUR. T1 - Preferential sequestration in vitro of BCR/ABL negative hematopoietic progenitor cells among cytokine nonresponsive CML marrow CD34+ cells. AU - Veena, P.. AU - Cornetta, K.. AU - Davidson, A.. AU - Agüero, B.. AU - McMahel, J.. AU - Traycoff, C. M.. AU - Srour, E. F.. PY - 1997/6/2. Y1 - 1997/6/2. N2 - It is believed that long-term cultures of CML marrow cells favor the outgrowth of BCR/ABL negative hematopoietic progenitor cells (HPC) and that this phenomenon may be enhanced with negative hematopoietic regulators which can maintain primitive HPC in a quiescent state. Proliferation of CML marrow CD34+ cells in primary short-term cultures, maintained in the presence or absence of macrophage inhibitory protein-1 alpha (MIP-1α), was tracked with the membrane dye PKH2. After 7 to 10 days it was possible to distinguish between cytokine responsive (CR) CD34+ cells (cells which had divided thus becoming PKH2(dim)) and cytokine nonresponsive (CNR) CD34+ cells (cells which had not ...
Hematopoietic progenitor cells, cord blood is used for blood cell transplantation procedures in patients with disorders that affect blood production. This medicine is derived from human blood that is collected from the umbilical cord and placenta. The hematopoietic progenitor cells go to the bone marrow where they become red blood cells, white blood cells, or platelets. These cells enter the blood stream and help restore low blood counts in patients with blood disorders. ...
in Haematologica (2010), 95(1), 47-56. Background Bone marrow (BM) mesenchymal stem cells (MSC) support proliferation and differentiation of hematopoietic progenitor cells (HPC) in vitro. Since they represent a rare subset of BM cells, MSC ... [more ▼]. Background Bone marrow (BM) mesenchymal stem cells (MSC) support proliferation and differentiation of hematopoietic progenitor cells (HPC) in vitro. Since they represent a rare subset of BM cells, MSC preparations for clinical purposes involves a preparative step of ex vivo multiplication. The aim of our study was to analyze the influence of culture duration on MSC supportive activity. DESIGN AND METHODS: MSC were expanded for up to 10 passages. MSC and CD34(+) cells were seeded in cytokine-free co-cultures after which the phenotype, clonogenic capacity and in vivo repopulating activity of harvested hematopoietic cells were assessed. RESULTS: Early passage MSC supported HPC expansion and differentiation toward both B lymphoid and myeloid ...
Identification of the soluble granulocyte-macrophage colony stimulating factor receptor protein in vivo and development of a soluable model of the high affinity cell surface receptor for granulocyte-macrophage colony stimulating ...
Abstract. Evidence has been provided recently that shows that high concentrations of cytokines can fulfill functions previously attributed to stromal cells, su
AppliedStemCell eCommerce Platform Human Bone Marrow Mononuclear Cells (DCM) [ASE-5071] - Catalog Number ASE-5071; ASE-5072; ASE-5073 Quantity 2.5 x 106 viable cells/mL; 10.0 x 106 viable cells/mL; 25.0 x 106 viable cells/mL Product Information Descriptio
Ploemacher R.E.; Nikkels P.G.J.; Molendijk W.J.; Brons N.H.C.; Brockbank K.G.M., 1984: Regulation of hemopoietic stem cell proliferation in mice carrying the s 1j allele
TY - JOUR. T1 - Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages. AU - Yokota, T.. AU - Oritani, K.. AU - Takahashi, I.. AU - Ishikawa, J.. AU - Matsuyama, Akifumi. AU - Ouchi, N.. AU - Kihara, S.. AU - Funahashi, T.. AU - Tenner, A. J.. AU - Tomiyama, Y.. AU - Matsuzawa, Y.. PY - 2000/9/1. Y1 - 2000/9/1. N2 - We investigated the functions of adiponectin, an adipocyte-specific secretory protein and a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. Adiponectin suppressed colony formation from colony-forming units (CFU)-granulocyte-macrophage, CFU-macrophage, and CFU-granulocyte, whereas it had no effect on that of burst-forming unitserythroid or mixed erythroid-myeloid CFU. In addition, adiponectin inhibited proliferation of 4 of 9 myeloid cell lines but did not suppress proliferation of erythroid or lymphoid cell lines except for one ...
ABCell-Bio offers its CD133+ hematopoietic progenitors carefully isolated from umbilical cord blood, an excellent alternative source of Hematopoietic Stem Cells. Our CD133+ hematopoietic progenitors are subject to many quality controls, certifying their virologic compliance, performance, purity and viability. We can supply CD133 + cells with a purity above 95%.
Assisted Reproductive Technologies and Haematopoietic stem cells Improvements for Quality and Safety throughout Europe ̶ an European Joint Action ...c
05). These data obviously showed that upresgulation of miR-451 might effectively enhance the sensitivity of A549 cells to DDP. Figure 5 Effect of miR-451 upregulation on the in. vitro sensitivity of A549 cells to DDP. A. Effects of various concentrations (0, 5, 10, 15, 20 and 25 μg/ml) of DDP on cells (mock A549, A549/miR-NC or A549/miR-451) for 12 h assessed by MTT assay. B. Effects of 5 μg/ml DDP on cells (mock A549, A549/miR-NC or A549/miR-451) for varied time length (0, 12, 24, 36 and 48 h) evaluated by MTT assays. C. Effects of 5 μg/ml DDP on colony formation of cells (mock A549, A549/miR-NC or A549/miR-451). All experiments were performed in triplicate, * P < 0.05. Upregulation of miR-451 enhances DDP-induced apoptosis of A549 cells The precise underlying mechanisms by which upregulation selleck of miR-451 enhances chemosensitivity of A549 cells to DDP were further investigated. Then, the apoptosis was detected by flow cytometric assay. As shown in Figure 6A, the apoptotic rare of ...
Medical information for Granulocyte Macrophage Colony Stimulating Factor on Pediatric Oncall including Mechanism, Indication, Contraindications, Dosing, Adverse Effect, Interaction.
Thanks, Manman , On Oct 12, 2016, at 4:57 AM, Vassil Vassilev via cfe-commits , ,[email protected], wrote: , , Author: vvassilev , Date: Wed Oct 12 06:57:08 2016 , New Revision: 284008 , , URL: http://llvm.org/viewvc/llvm-project?rev=284008&view=rev , Log: , Reinstate r283887 and r283882. , , Original message: , [modules] PR28752: Do not instantiate variable declarations which are not , visible. , , https://reviews.llvm.org/D24508 , , Patch developed in collaboration with Richard Smith! , , Added: , cfe/trunk/test/Modules/Inputs/PR28752/ , cfe/trunk/test/Modules/Inputs/PR28752/Subdir1/ , cfe/trunk/test/Modules/Inputs/PR28752/Subdir1/b.h , cfe/trunk/test/Modules/Inputs/PR28752/Subdir1/c.h , cfe/trunk/test/Modules/Inputs/PR28752/Subdir1/module.modulemap , cfe/trunk/test/Modules/Inputs/PR28752/a.h , cfe/trunk/test/Modules/Inputs/PR28752/module.modulemap , cfe/trunk/test/Modules/Inputs/PR28752/vector , cfe/trunk/test/Modules/pr28752.cpp , Modified: , cfe/trunk/include/clang/AST/Decl.h , ...
Thank you for your inquiry. It is not a dual strain, but you can see some differences when the colonies are small. It can be stress or volume size plated. 10ul is a lot of yeast to plate so there can be nutrient differences that each colony is receiving. The best way is to grow a dilute solution to giant colony size; I think there would be some protocols on the web if you want to try that. When they are bigger you will be able to better see if there are actual morphological differences ...
Protein energy malnutrition (PEM) is a syndrome that often results in immunodeficiency coupled with pancytopenia. Hemopoietic tissue requires a high nutrient supply and the proliferation, differentiation and maturation of cells occur in a constant and balanced manner, sensitive to the demands of specific cell lineages and dependent on the stem cell population. In the present study, we evaluated the effect of PEM on some aspects of hemopoiesis, analyzing the cell cycle of bone marrow cells and the percentage of progenitor cells in the bone marrow. Two-month-old male Swiss mice (N = 7-9 per group) were submitted to PEM with a low-protein diet (4%) or were fed a control diet (20% protein) ad libitum. When the experimental group had lost about 20% of their original body weight after 14 days, we collected blood and bone marrow cells to determine the percentage of progenitor cells and the number of cells in each phase of the cell cycle. Animals of both groups were stimulated with 5-fluorouracil. Blood ...
OBJECTIVE: The current therapy of myelodysplastic syndrome (MDS) is unsatisfactory and comprises mainly supportive treatment or antileukemic chemotherapy. Recent studies about successful immunosuppressive therapy suggest an autoimmune mechanism in subtypes of myelodysplastic syndrome. PATIENTS AND METHODS: To investigate this hypothesis, bone marrow mononuclear cells (MNC) from 15 patients with low-grade MDS, refractory anemia, and refractory anemia with ringed sideroblasts (RA and RARS), and from 7 normal donors were depleted of CD2(+), CD5(+), and CD7(+) lymphocytes using magnetic cell sorting. Depleted and nondepleted MNC were seeded onto irradiated allogeneic bone marrow stroma and the generation of colony-forming-cells (CFC), the clonal origin of hemopoietic progenitor cells in long-term bone marrow culture (LTC), was compared. RESULTS: The capacity of MNC from 7 healthy donors to generate hemopoiesis remained unchanged in the lymphocyte-depleted LTC. In contrast, cultures initiated with ...
Murine acquired immunodeficiency syndrome (MAIDS) induced by defective LP-BM5 murine leukemia virus is a disease with many similarities to human AIDS. Previous studies indicated that the depressed hematopoiesis observed in LP-BM5-infected marrow cultures may be attributable to a defect of hematopoietic stroma. We report here the generation of permanent stromal cell lines from noninfected and LP-BM5-infected marrow cultures. Retrovirus infection was confirmed by polymerase chain reaction for viral genome. The ability of these cell lines to support in vitro hematopoiesis was studied. Results indicated that, when cocultured with normal or infected nonadherent mononuclear cells, noninfected cell lines efficiently supported the production of hematopoietic precursors, whereas viral-infected cell lines induced suppression of both normal and viral-infected progenitors. Expression of cytokine genes in stromal cell lines was also examined. All cell lines expressed equivalent levels of transcripts for stem ...

Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting) -...Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting) -...

Home/Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony ... Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting). ... Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting). ... To get support on Synoptics systems you can visit our support helpdesk, send us an email, or fill in an online form:. ...
more infohttps://www.synbiosis.com/technical-paper/simplified-method-automatically-count-bacterial-colony-forming-unit-opsonophagocytosis-assay-bacterial-colony-counting/

Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting) -...Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting) -...

Home/Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony ... Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting). ... Simplified method to automatically count bacterial colony forming unit (Opsonophagocytosis assay, Bacterial colony counting). ... To get support on Synoptics systems you can visit our support helpdesk, send us an email, or fill in an online form:. ...
more infohttps://www.synbiosis.com/technical-paper/simplified-method-automatically-count-bacterial-colony-forming-unit-opsonophagocytosis-assay-bacterial-colony-counting-2/

Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of...Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of...

Assay for colony-forming units. Assays for t-CFUs provide a convenient means of assessing the proliferative capacity that MSCs ... CFU assay) as a guide to the growth potential of the cultures (Figure 1B). For the traditional colony-forming unit assay (t-CFU ... Propagation and senescence of human marrow stromal cells in culture: a simple colony-forming assay identifies samples with the ... B) t-CFU assays were performed after the 12 days incubation in the medium indicated. (C) t-CFU assays and sc-CFU assays were ...
more infohttp://www.bloodjournal.org/content/103/5/1662?sso-checked=true

Targeting Protein Tyrosine Phosphatase SHP2 for the Treatment of PTPN11-Associated Malignancies | Molecular Cancer TherapeuticsTargeting Protein Tyrosine Phosphatase SHP2 for the Treatment of PTPN11-Associated Malignancies | Molecular Cancer Therapeutics

Colony-forming unit assay. Freshly harvested mouse bone marrow cells or patient splenocytes (2 × 104 cells/mL) were assayed for ... colony forming units (CFU) in 0.9% methylcellulose IMDM containing 30% FBS, glutamine (10−4 mol/L), β-mercaptoethanol (3.3 × 10 ... In the presence of GM-CSF, the colony-forming capabilities of PTPN11E76K/+ myeloid progenitors were more sensitive to #220-324 ... Colonies were enumerated 7 days later and normalized against the number of colonies derived from WT control cells without #220- ...
more infohttp://mct.aacrjournals.org/content/12/9/1738.long

Hematopoietic Progenitor Cell Rolling in Bone Marrow Microvessels: Parallel Contributions by Endothelial Selectins and Vascular...Hematopoietic Progenitor Cell Rolling in Bone Marrow Microvessels: Parallel Contributions by Endothelial Selectins and Vascular...

Culture Colony-Forming Unit Assay.. Frontoparietal bones, femora, and brains of five mice were ground using a mortar and pestle ... pathway and one or more unidentified lectins determine in part the recovery of colony-forming units from the BM of irradiated ... D) Colony-forming capacity of MNCs from murine brain, skull, and femur. MNCs isolated from brains, skullcaps, and femora of ... The difference in the frequency of colonies in CFU-C assays using MNCs from skulls and femora was not statistically significant ...
more infohttp://jem.rupress.org/content/188/3/465?ijkey=17bd27d3904240cb09d40a740d577720c2ef09f8&keytype2=tf_ipsecsha

Frontiers | Mycobacterium bovis Induces Endoplasmic Reticulum Stress Mediated-Apoptosis by Activating IRF3 in a Murine...Frontiers | Mycobacterium bovis Induces Endoplasmic Reticulum Stress Mediated-Apoptosis by Activating IRF3 in a Murine...

M. bovis Infection and Colony-Forming Unit Assay. Macrophages were infected in vitro with M. bovis at an MOI of 10 and ... The JC-1-aggregate form, indicating normal ΔΨm, appears red and the monomeric form, indicating low ΔΨm (i.e., disrupted ... assay (Figure 6A). After infection with M. bovis for 24 h, green fluorescence (JC-1 monomer form) increased and red ... All assays were performed on three separate occasions in triplicate at each time. Results are expressed as means ± S.D. All ...
more infohttps://www.frontiersin.org/articles/10.3389/fcimb.2016.00182/full

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Colony-forming unit assay. A colony-forming unit (CFU) assay was carried out to show blast elimination in a long-term coculture ... 4C). Furthermore, a specific inhibition of colony formation was observed in CFU assays after 14 days when leukemia blasts were ... Colonies were counted on day 14 and relative inhibition of colony formation calculated in comparison with nonspecific T cells ... IFN-γ enzyme-linked immunospot assay. Peptide recognition was tested in an IFN-γ-enzyme-linked immunospot (ELISpot) assay (23 ...
more infohttp://clincancerres.aacrjournals.org/content/19/18/5079

Endothelium-targeted human Delta-like 1 enhances the regeneration and homing of human cord blood stem and progenitor cells |...Endothelium-targeted human Delta-like 1 enhances the regeneration and homing of human cord blood stem and progenitor cells |...

Colony-forming units (CFU) assay. CFU assay was performed by mixing freshly isolated or cultured hematopoietic cells with ... were used for colony-forming assay (n = 4). f Photomicrographs of human hemopoietic cell colonies. Scale bar represented 200 μm ... 5c, d). Colony-forming assay of BM cells showed higher BM CFU counts in hD1R-treated mice than in PBS-treated ones (Fig. 5e). ... The results showed that the total number of colonies and the numbers of burst-forming unit-erythroid (BFU-E), CFU-granulocyte ...
more infohttps://translational-medicine.biomedcentral.com/articles/10.1186/s12967-015-0761-0

Multilevel defects in the hematopoietic niche in essential thrombocythemia | HaematologicaMultilevel defects in the hematopoietic niche in essential thrombocythemia | Haematologica

Colony-forming unit (CFU) assay. To evaluate the capacity of MSC to sustain normal hematopoiesis, a CFU assay was performed ... colony-forming unit-erythroid; CFU-GM: colony-forming unit-granulocyte and macrophage; CFU-Mix: colony-forming units mixed; CFU ... colony-forming unit-erythroid; CFU-GM: colony-forming unit-granulocyte and macrophage; CFU-Total: total colony-forming units; ... total colony-forming units; CFU-MK: colony-forming unit-megakaryocyte; SEM: standard error of mean. ...
more infohttp://www.haematologica.org/content/105/3/661

Isolation of functionally distinct mesenchymal stem cell subsets using antibodies against CD56, CD271, and mesenchymal stem...Isolation of functionally distinct mesenchymal stem cell subsets using antibodies against CD56, CD271, and mesenchymal stem...

Colony forming unit-fibroblast assay. CFU-F assays were performed by plating either 1×105 unselected or 500-5,000 FACS-selected ... MSC can be identified by their ability to form colony forming units-fibroblast (CFU-F) in vitro.2,7 However, these cells are ... The growth of mesenchymal stem cells was monitored by colony formation unit fibroblast (CFU-F) assays. The differentiation of ... could be expanded and used for differentiation assays, indicating that only these colonies are derived from immature MSC. ...
more infohttp://www.haematologica.org/content/94/2/173

Frontiers | Myrtenol Attenuates MRSA Biofilm and Virulence by Suppressing sarA Expression Dynamism | MicrobiologyFrontiers | Myrtenol Attenuates MRSA Biofilm and Virulence by Suppressing sarA Expression Dynamism | Microbiology

... sarA and sarA mediated virulence genes upon myrtenol treatment which is well correlated with results of phenotypic assays. Thus ... was evidenced by trypan blue and Alamar blue assays. Transcriptional analysis unveiled the down-regulation of global regulator ... Colony Forming Unit (CFU) Assay. CFU assay was performed to determine the cell count variation between control and myrtenol ... Mature Biofilm Assay. To determine the effect of myrtenol on mature biofilm, MRSA was allowed to form biofilm on glass slides ...
more infohttps://www.frontiersin.org/articles/10.3389/fmicb.2019.02027/full

A novel role for lipid droplets in the organismal antibacterial response | eLifeA novel role for lipid droplets in the organismal antibacterial response | eLife

... gel overlay assays as well as disc diffusion assays.. The colony forming units (CFU) assay. The antibacterial activity of LDs ... Antibacterial assays. To evaluate the antibacterial property of purified LDs/LD components, we performed colony forming assays ... C). Representative plates in a colony forming assay, showing bacterial colonies on agar plates streaked with cytosolic extract ... Quantification of colony forming assay in A. Each bar represents the mean number of observed colonies, in three independent ...
more infohttps://elifesciences.org/articles/00003

Category:Cell biology - Wikimedia CommonsCategory:Cell biology - Wikimedia Commons

Colony-forming units assay‎ (21 F). *. ► Cytogenetics‎ (4 C, 21 F). *. ► Cytological techniques‎ (3 C, 54 F) ... Colonies of Madin-Darby Canine Kidney cells grown in tissue culture.jpg 1,030 × 1,030; 263 KB. ... Astrocyte and Glioblastoma Invasion Assay performed after 3D culturing by MLM.tiff 687 × 536; 363 KB. ...
more infohttps://commons.wikimedia.org/wiki/Category:Cell_biology

miR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis | eLifemiR-142 orchestrates a network of actin cytoskeleton regulators during megakaryopoiesis | eLife

Colony-forming unit assays. Mouse BM cells were harvested via flushing of the long bones with Dulbecco modified Eagle medium ( ... To further confirm the expansion of miR-142−/− MkPs, we employed a colony forming unit-megakaryocyte (CFU-MK) assay that ... G) Left panel, CFU-MK assays demonstrate increased miR-142−/− MK numbers per colony. Scale bars, 50 μm. Right panel, increased ... Colonies containing ,3 MKs were counted as CFU-MKs. Duplicate assays were performed for each mouse. At least two mice were ...
more infohttps://elifesciences.org/articles/01964

A putative mesenchymal stem cells population isolated from adult huma… - EnglishA putative mesenchymal stem cells population isolated from adult huma… - English

Colony-forming units (CFU) assay and cell cloning. For colonies, cells were plated at a density of 150 cells/10 cm dish. After ... Staining was performed using 0.3% Oil Red O solution (Sigma-Aldrich). For quantitative as- say, Oil Red O bound to lipid ... Reversible commitment to differentiation by human multipotent stromal cells in single-cell-derived colonies, Exp. Hematol. 36 ( ...
more infohttps://www.slideshare.net/JustinGaines1/gonzalez-et-al-2009

Plus itPlus it

Colony-forming unit assays show suppression of lymphoid progenitors by each PAH within 6 h but a subsequent recovery, ... colony-forming unit. Cxcl. chemokine (C-X-C motif) ligand. DBP. dibenzo(a,l)pyrene. DMBA. 7,12-dimethylbenz(a)anthracene. FBS. ... Hematopoiesis can be recapitulated in vitro by using colony-forming unit (CFU) assays that specifically provide for the ... Bone Marrow Cell ColonyForming Unit Assays.. Freshly isolated bone marrow cells were resuspended at concentrations of 105 cells ...
more infohttp://molpharm.aspetjournals.org/content/79/4/724

Osteoclasts Are Required for Hematopoietic Stem and Progenitor Cell Mobilization but Not for Stress Erythropoiesis in...Osteoclasts Are Required for Hematopoietic Stem and Progenitor Cell Mobilization but Not for Stress Erythropoiesis in...

Colony-Forming Unit Assays. Single-cell suspensions from the spleen and BM were prepared at 1 × 106 and 2 × 105 cells/mL, ... The cultures were incubated at 37°C, 5% CO2 in air, and 95% humidity for 8 days after which Colony-Forming Unit Macrophages ( ... 0.001, resp.). Analysis of progenitors Colony Forming Unit-Macrophage (CFU-M) did not reveal major modifications in the BM of ... the numbers of Colony Forming Unit-Macrophage (CFU-M) were determined in 12-day bone marrow (c) and spleen (d) cultures using ...
more infohttps://www.hindawi.com/journals/mi/2016/3909614/

Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells | Journal of Translational Medicine |...Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells | Journal of Translational Medicine |...

PL lots were obtained by combining 2 6-unit PLT pools in additive solution (AS) following a transfusional-based procedure ... Here, we describe a plasma poor pathogen-free platelet lysate obtained by pooling 12 platelet (PLT) units, to produce a ... Colony-forming unit-fibroblast (CFU-F) assay. Colony-forming-unit assay was performed by plating 2 × 105 BM-MNC/ml in D-MEM ... In detail, two pools of six PI-PLT units were connected each other under sterile conditions to obtain a pool of 12 PI-PLT units ...
more infohttps://translational-medicine.biomedcentral.com/articles/10.1186/1479-5876-12-28

Effects of Maternal Hypoxia during Pregnancy on Bone Development in Offspring: A Guinea Pig ModelEffects of Maternal Hypoxia during Pregnancy on Bone Development in Offspring: A Guinea Pig Model

Colony Forming Unit-Fibroblast Assay. To determine treatment effects on the size of the osteoprogenitor cell pool, a colony ... forming unit-fibroblast (CFU-f) assay and alkaline phosphatase (ALP, an osteoprogenitor cell marker) staining were performed ... CFU-F colonies formed) was not affected by MH. The effect of MH on the osteogenic potential in the fetal offspring has not ... The number of positive colonies (osteoprogenitor cell pool in the bone marrow) was expressed as CFU-f ALP+ colonies per 2 × 106 ...
more infohttps://www.hindawi.com/journals/ije/2014/916918/

Educational MaterialsEducational Materials

MethoCult™ Media for Performing Hematopoietic Colony-Forming Unit (CFU) Assays Product Type:. Cell Culture Media and ... ClonaCell™-HY Medium D for Robust Colony Formation During Semi-Solid Cloning Product Type:. Cell Culture Media and Supplements ... Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays Cell Type:. Hematopoietic Stem and Progenitor ...
more infohttps://www.stemcell.com/technical-resources/educational-materials.html?dir=desc&limit=36&order=position&p=2

Colony-Forming Cell Assay Detecting the Co-Expression of JAK2V617F and BCR-ABL1 in the Same Clone: A Case Report.Colony-Forming Cell Assay Detecting the Co-Expression of JAK2V617F and BCR-ABL1 in the Same Clone: A Case Report.

Colony-forming Units Assay. A cytologic technique for measuring the functional capacity of stem cells by assaying their ... Each colony (i.e., microbial colony-forming unit) represents the progeny of a single cell in the original inoculum. The method ... Summary of "Colony-Forming Cell Assay Detecting the Co-Expression of JAK2V617F and BCR-ABL1 in the Same Clone: A Case Report." ... Colony-Forming Cell Assay Detecting the Co-Expression of JAK2V617F and BCR-ABL1 in the Same Clone: A Case Report.. 08:00 EDT ...
more infohttps://www.bioportfolio.com/resources/pmarticle/2340113/Colony-Forming-Cell-Assay-Detecting-the-Co-Expression-of-JAK2V617F-and-BCR.html

Apoptotic cell death in TrkA-overexpressing cells: kinetic regulation of ERK phosphorylation and caspase-7 activation.Apoptotic cell death in TrkA-overexpressing cells: kinetic regulation of ERK phosphorylation and caspase-7 activation.

Colony-Forming Units Assay. Enzyme Activation. Extracellular Signal-Regulated MAP Kinases / metabolism*. Flow Cytometry. G1 ... TrkA-mediated cell death was shown by the annexin-V binding assay to be, at least in part, apoptotic in both SK-N-MC and U2OS ... Furthermore, the truncated form (p18) of Bax accumulated in the TrkA-induced cells, suggesting that TrkA induces mitochondria- ...
more infohttp://www.biomedsearch.com/nih/Apoptotic-cell-death-in-TrkA/18511888.html

Characterization of the erythropoiesis in myelodysplasia by means of ferrokinetic studies, in vitro erythroid colony formation...Characterization of the erythropoiesis in myelodysplasia by means of ferrokinetic studies, in vitro erythroid colony formation...

... a discrepancy is observed between the decreased in vitro erythroid colony formation and the normal or increased number of ... Colony-Forming Units Assay. Erythropoiesis*. Erythropoietin / blood. Hematocrit. Hematopoietic Stem Cells / pathology*, ... Characterization of the erythropoiesis in myelodysplasia by means of ferrokinetic studies, in vitro erythroid colony formation ... and erythroid in vitro colony formation were performed in 24 patients with RA and five patients with RARS. These results were ...
more infohttp://www.biomedsearch.com/nih/Characterization-erythropoiesis-in-myelodysplasia-by/9529128.html

Non-Embryonic Stem Cell Core - University of Mississippi Medical CenterNon-Embryonic Stem Cell Core - University of Mississippi Medical Center

Non-adherence assays (mammospheres, sarcospheres)Extracellular vesicles characterization (electron microscopy, micro BCA, ... isolation and purificationQuality assurance Differentiation assays for MSCColony forming units assayCell surface markers (FACS ... Colony forming units assay. *Cell surface markers (FACS analysis). *Non-adherence assays (mammospheres, sarcospheres) ...
more infohttps://www.umc.edu/cancerinstitute/Cancer-Research/Core-Facilities/Non-Embryonic-Stem-Cell-Core.html

Translational Trial Development and Support Laboratory | Translational Core LaboratoryTranslational Trial Development and Support Laboratory | Translational Core Laboratory

The Translational Trial Development and Support Laboratory at Cincinnati Childrens supports clinical assay development from ... technology developed in academic laboratories and provides assay development and testing support for early phase clinical ... Hematopoietic cell colony forming unit (CFU) assay. * Protein HPLC. * Gene expression by qPCR or flow cytometry ... The following assays are offered:. * In vitro immortalization (IVIM) assay to assess genotoxicity of integrating viral vectors ...
more infohttps://www.cincinnatichildrens.org/research/cores/translational-core-laboratory/translational-trial-development-support-laboratory
  • The MSCs obtained from 5 different strains of mice were similar to human and rat MSCs in that they expanded more rapidly if plated at very low density, formed single-cell-derived colonies, and readily differentiated into either adipocytes, chondrocytes, or mineralizing cells. (bloodjournal.org)
  • However, contradictory evidences demonstrate that inhibition of osteoclast activity by bisphosphonate does not impair HSPC mobilization in response to Granulocyte-Colony Stimulating Factor (G-CSF) treatment [ 1 , 10 ], suggesting that osteoclasts may only intervene in certain types of hematopoietic stresses. (hindawi.com)
  • Longitudinal bone growth before and after birth takes place in the growth plate located at the epiphyses of long bones via an endochondral ossification process in which a hypertrophic cartilage template is made, calcified, and invaded by blood vessels and then converted to trabecular bone through the action of bone forming cells, osteoblasts, and bone resorbing cells, osteoclasts [ 1 ]. (hindawi.com)
  • An ex vivo assay for examining the clonogenic potential of multipotent marrow cells was later reported in the 1970s by Friedenstein and colleagues. (wikipedia.org)
  • Here, we describe a plasma poor pathogen-free platelet lysate obtained by pooling 12 platelet (PLT) units, to produce a standardized and safe supplement for clinical-grade expansion of MSC. (biomedcentral.com)
  • The Translational Trial Development and Support Laboratory (TTDSL) was established in 2002 to provide the facilities and expertise to support clinical assay development from technology developed in academic laboratories. (cincinnatichildrens.org)
  • We provide support in optimizing a standard operating procedure (SOP), validating the assay at a level congruent with CAP regulations, and then offering the test through the TTDSL or in conjunction with another Cincinnati Children's clinical laboratory. (cincinnatichildrens.org)
  • The TTDSL has effectively supported 11 Phase I/II gene therapy clinical trials by designing tailored analytical assays and acquiring sponsor-developed assays via technology transfer. (cincinnatichildrens.org)
  • That's why we have developed products specifically designed to automate colony counting and inhibition zone measurement, to relieve you of the tedium involved with these repetitive manual tasks. (synbiosis.com)
  • We produce a world leading range of automated colony counting and zone sizing products for the microbiology laboratory. (synbiosis.com)