Collagenases: Enzymes that catalyze the degradation of collagen by acting on the peptide bonds.Microbial Collagenase: A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC 3.4.24.3.Matrix Metalloproteinase 8: A member of the MATRIX METALLOPROTEINASES that cleaves triple-helical COLLAGEN types I, II, and III.Clostridium histolyticum: A species of gram-positive, strongly proteolytic bacteria in the family Clostridiaceae. It contains several forms of COLLAGENASE whose action can lead to GAS GANGRENE in humans and HORSES.Matrix Metalloproteinase 13: A secreted matrix metalloproteinase that plays a physiological role in the degradation of extracellular matrix found in skeletal tissues. It is synthesized as an inactive precursor that is activated by the proteolytic cleavage of its N-terminal propeptide.Matrix Metalloproteinase 1: A member of the metalloproteinase family of enzymes that is principally responsible for cleaving FIBRILLAR COLLAGEN. It can degrade interstitial collagens, types I, II and III.Matrix Metalloproteinase Inhibitors: Compounds that inhibit the enzyme activity or activation of MATRIX METALLOPROTEINASES.Collagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Tissue Inhibitor of Metalloproteinases: A family of secreted protease inhibitory proteins that regulates the activity of SECRETED MATRIX METALLOENDOPEPTIDASES. They play an important role in modulating the proteolysis of EXTRACELLULAR MATRIX, most notably during tissue remodeling and inflammatory processes.Metalloendopeptidases: ENDOPEPTIDASES which use a metal such as ZINC in the catalytic mechanism.Gelatinases: A class of enzymes that catalyzes the degradation of gelatin by acting on the peptide bonds. EC 3.4.24.-.Tissue Inhibitor of Metalloproteinase-2: A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It is a 21-kDa nonglycosylated protein found in tissue fluid and is secreted as a complex with progelatinase A by human fibroblast and uncomplexed from alveolar macrophages. An overexpression of TIMP-2 has been shown to inhibit invasive and metastatic activity of tumor cells and decrease tumor growth in vivo.Matrix Metalloproteinases: A family of zinc-dependent metalloendopeptidases that is involved in the degradation of EXTRACELLULAR MATRIX components.Gelatin: A product formed from skin, white connective tissue, or bone COLLAGEN. It is used as a protein food adjuvant, plasma substitute, hemostatic, suspending agent in pharmaceutical preparations, and in the manufacturing of capsules and suppositories.Protease Inhibitors: Compounds which inhibit or antagonize biosynthesis or actions of proteases (ENDOPEPTIDASES).Matrix Metalloproteinase 9: An endopeptidase that is structurally similar to MATRIX METALLOPROTEINASE 2. It degrades GELATIN types I and V; COLLAGEN TYPE IV; and COLLAGEN TYPE V.Tissue Inhibitor of Metalloproteinase-1: A member of the family of TISSUE INHIBITOR OF METALLOPROTEINASES. It is a N-glycosylated protein, molecular weight 28 kD, produced by a vast range of cell types and found in a variety of tissues and body fluids. It has been shown to suppress metastasis and inhibit tumor invasion in vitro.Matrix Metalloproteinase 3: An extracellular endopeptidase of vertebrate tissues similar to MATRIX METALLOPROTEINASE 1. It digests PROTEOGLYCAN; FIBRONECTIN; COLLAGEN types III, IV, V, and IX, and activates procollagenase. (Enzyme Nomenclature, 1992)Matrix Metalloproteinase 2: A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.Matrix Metalloproteinases, Membrane-Associated: Matrix metalloproteinases that are associated with the CELL MEMBRANE, either through transmembrane domains or GLYCOSYLPHOSPHATIDYLINOSITOL ANCHORS. Membrane-type matrix metalloproteinases may act within the pericellular environment to influence the process of CELL MIGRATION.Substrate Specificity: A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.Clostridium: A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.Pepsin A: Formed from pig pepsinogen by cleavage of one peptide bond. The enzyme is a single polypeptide chain and is inhibited by methyl 2-diaazoacetamidohexanoate. It cleaves peptides preferentially at the carbonyl linkages of phenylalanine or leucine and acts as the principal digestive enzyme of gastric juice.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Cartilage, Articular: A protective layer of firm, flexible cartilage over the articulating ends of bones. It provides a smooth surface for joint movement, protecting the ends of long bones from wear at points of contact.Molecular Weight: The sum of the weight of all the atoms in a molecule.Extracellular Matrix: A meshwork-like substance found within the extracellular space and in association with the basement membrane of the cell surface. It promotes cellular proliferation and provides a supporting structure to which cells or cell lysates in culture dishes adhere.RNA, Messenger: RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.Endopeptidases: A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.Gene Expression Regulation, Enzymologic: Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in enzyme synthesis.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Blotting, Northern: Detection of RNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.Electrophoresis, Polyacrylamide Gel: Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.Sequence Homology, Amino Acid: The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.Neutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Kinetics: The rate dynamics in chemical or physical systems.Recombinant Proteins: Proteins prepared by recombinant DNA technology.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Enzyme Activation: Conversion of an inactive form of an enzyme to one possessing metabolic activity. It includes 1, activation by ions (activators); 2, activation by cofactors (coenzymes); and 3, conversion of an enzyme precursor (proenzyme or zymogen) to an active enzyme.Enzyme-Linked Immunosorbent Assay: An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Microscopy, Electron: Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.Trypsin: A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.Dupuytren Contracture: A fibromatosis of the palmar fascia characterized by thickening and contracture of the fibrous bands on the palmar surfaces of the hand and fingers. It arises most commonly in men between the ages of 30 and 50.Collagen Type XI: A fibrillar collagen found primarily in interstitial CARTILAGE. Collagen type XI is heterotrimer containing alpha1(XI), alpha2(XI) and alpha3(XI) subunits.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Gold Sodium Thiosulfate: A sodium salt of gold thiosulfate. It has uses like the ORGANOGOLD COMPOUNDS.

Bone resorption induced by parathyroid hormone is strikingly diminished in collagenase-resistant mutant mice. (1/1761)

Parathyroid hormone (PTH) stimulates bone resorption by acting directly on osteoblasts/stromal cells and then indirectly to increase differentiation and function of osteoclasts. PTH acting on osteoblasts/stromal cells increases collagenase gene transcription and synthesis. To assess the role of collagenase in the bone resorptive actions of PTH, we used mice homozygous (r/r) for a targeted mutation (r) in Col1a1 that are resistant to collagenase cleavage of type I collagen. Human PTH(1-34) was injected subcutaneously over the hemicalvariae in wild-type (+/+) or r/r mice four times daily for three days. Osteoclast numbers, the size of the bone marrow spaces and periosteal proliferation were increased in calvariae from PTH-treated +/+ mice, whereas in r/r mice, PTH-induced bone resorption responses were minimal. The r/r mice were not resistant to other skeletal effects of PTH because abundant interstitial collagenase mRNA was detected in the calvarial periosteum of PTH-treated, but not vehicle-treated, r/r and +/+ mice. Calcemic responses, 0.5-10 hours after intraperitoneal injection of PTH, were blunted in r/r mice versus +/+ mice. Thus, collagenase cleavage of type I collagen is necessary for PTH induction of osteoclastic bone resorption.  (+info)

Activation of c-Jun N-terminal kinase 1 by UV irradiation is inhibited by wortmannin without affecting c-iun expression. (2/1761)

Activation of c-Jun N-terminal kinases (JNKs)/stress-activated protein kinases is an early response of cells upon exposure to DNA-damaging agents. JNK-mediated phosphorylation of c-Jun is currently understood to stimulate the transactivating potency of AP-1 (e.g., c-Jun/c-Fos; c-Jun/ATF-2), thereby increasing the expression of AP-1 target genes. Here we show that stimulation of JNK1 activity is not a general early response of cells exposed to genotoxic agents. Treatment of NIH 3T3 cells with UV light (UV-C) as well as with methyl methanesulfonate (MMS) caused activation of JNK1 and an increase in c-Jun protein and AP-1 binding activity, whereas antineoplastic drugs such as mafosfamide, mitomycin C, N-hydroxyethyl-N-chloroethylnitrosourea, and treosulfan did not elicit this response. The phosphatidylinositol 3-kinase inhibitor wortmannin specifically blocked the UV-stimulated activation of JNK1 but did not affect UV-driven activation of extracellular regulated kinase 2 (ERK2). To investigate the significance of JNK1 for transactivation of c-jun, we analyzed the effect of UV irradiation on c-jun expression under conditions of wortmannin-mediated inhibition of UV-induced stimulation of JNK1. Neither the UV-induced increase in c-jun mRNA, c-Jun protein, and AP-1 binding nor the activation of the collagenase and c-jun promoters was affected by wortmannin. In contrast, the mitogen-activated protein kinase/ERK kinase inhibitor PD98056, which blocked ERK2 but not JNK1 activation by UV irradiation, impaired UV-driven c-Jun protein induction and AP-1 binding. Based on the data, we suggest that JNK1 stimulation is not essential for transactivation of c-jun after UV exposure, whereas activation of ERK2 is required for UV-induced signaling leading to elevated c-jun expression.  (+info)

Recruitment of the retinoblastoma protein to c-Jun enhances transcription activity mediated through the AP-1 binding site. (3/1761)

The retinoblastoma susceptibility gene product (RB) is a transcriptional modulator. One of the targets for this modulator effect is the AP-1 binding site within the c-jun and collagenase promoters. The physical interactions between RB and c-Jun were demonstrated by co-immunoprecipitation of these two proteins using anti-c-Jun or anti-RB antisera, glutathione S-transferase affinity matrix binding assays in vitro, and electrophoretic mobility shift assays. The C-terminal site of the leucine zipper of c-Jun mediated the interaction with RB. Although the B-pocket domain of RB alone bound to c-Jun, a second c-Jun binding site in the RB was also suggested. Mammalian two-hybrid-based assay provided corroborative evidence that transactivation of gene expression by RB required the C-terminal region of c-Jun. We conclude that RB enhances transcription activity mediated through the AP-1 binding site. Adenovirus E1A or human papillomavirus E7 inhibits RB-mediated transcription activity. These data reveal that the interactions between these two distinct classes of oncoproteins RB and c-Jun may be involved in controlling cell growth and differentiation mediated by transcriptional regulation.  (+info)

Murine matrix metalloproteinase 9 gene. 5'-upstream region contains cis-acting elements for expression in osteoclasts and migrating keratinocytes in transgenic mice. (4/1761)

Knowledge about the regulation of cell lineage-specific expression of extracellular matrix metalloproteinases is limited. In the present work, the murine matrix metalloproteinase 9 (MMP-9) gene was shown to contain 13 exons, and the 2.8-kilobase pair upstream region was found to contain several common promoter elements including a TATA box-like motif, three GC boxes, four AP-1-like binding sites, an AP-2 site, and three PEA3 consensus sequences that may be important for basic activity of the gene. In order to identify cell-specific regulatory elements, constructs containing varying lengths of the upstream region in front of a LacZ reporter gene were made and studied for expression in transgenic mice generated by microinjection into fertilized oocytes. Analyses of the mice revealed that the presence of sequences between -2722 and -7745 allowed for expression in osteoclasts and migrating keratinocytes, i. e. cells that have been shown to normally express the enzyme in vivo. The results represent the first in vivo demonstration of the location of cell-specific control elements in a matrix metalloproteinase gene and show that element(s) regulating most cell-specific activities of 92-kDa type collagenase are located in the -2722 to -7745 base pair region.  (+info)

Expression and tissue localization of membrane-type 1, 2, and 3 matrix metalloproteinases in human astrocytic tumors. (5/1761)

Three different membrane-type matrix metalloproteinases (MT1-, MT2-, and MT3-MMPs) are known to activate in vitro the zymogen of MMP-2 (pro-MMP-2, progelatinase A), which is one of the key MMPs in invasion and metastasis of various cancers. In the present study, we have examined production and activation of pro-MMP-2, expression of MT1-, MT2-, and MT3-MMPs and their correlation with pro-MMP-2 activation, and localization of MMP-2, MT1-MMP, and MT2-MMP in human astrocytic tumors. The sandwich enzyme immunoassay demonstrates that the production levels of pro-MMP-2 in the anaplastic astrocytomas and glioblastomas are significantly higher than that in the low-grade astrocytomas (P<0.05 and P<0.01, respectively), metastatic brain tumors (P<0.05), or normal brains (P<0.01). Gelatin zymography indicates that the pro-MMP-2 activation ratio is significantly higher in the glioblastomas than in other astrocytic tumors (P<0.01), metastatic brain tumors (P<0.01), and normal brains (P<0.01). The quantitative reverse transcription polymerase chain reaction analyses demonstrate that MT1-MMP and MT2-MMP are expressed predominantly in glioblastoma tissues (17/17 and 12/17 cases, respectively), and their expression levels increase significantly as tumor grade increases. MT3-MMP is detectable in both astrocytic tumor and normal brain tissues, but the mean expression level is approximately 50-fold lower compared with that of MT1-MMP and MT2-MMP in the glioblastomas. The activation ratio of pro-MMP-2 correlates directly with the expression levels of MT1-MMP and MT2-MMP but not MT3-MMP. In situ hybridization indicates that neoplastic astrocytes express MT1-MMP and MT2-MMP in the glioblastoma tissues (5/5 cases and 5/5 cases, respectively). Immunohistochemically, MT1-MMP and MT2-MMP are localized to the neoplastic astrocytes in glioblastoma samples (17/17 cases and 12/17 cases, respectively), which are also positive for MMP-2. In situ zymography shows gelatinolytic activity in the glioblastoma tissues but not in the normal brain tissues. These results suggest that both MT1-MMP and MT2-MMP play a key role in the activation of pro-MMP-2 in the human malignant astrocytic tumors and that the gelatinolytic activity is involved in the astrocytic tumor invasion.  (+info)

Collagenase-3 (MMP-13) is expressed by tumor cells in invasive vulvar squamous cell carcinomas. (6/1761)

Collagenase-3 (MMP-13) is a human matrix metalloproteinase specifically expressed by invading tumor cells in squamous cell carcinomas (SCCs) of the head and neck. Here, we have further elucidated the role of MMP-13 in tumor invasion by examining its expression in invasive malignant tumors of the female genital tract. Using in situ hybridization, expression of MMP-13 mRNA was detected in 9 of 12 vulvar SCCs, primarily in tumor cells, but not in intact vulvar epithelium, in cervical SCCs (n = 12), or in endometrial (n = 11) or ovarian adenocarcinomas (n = 8). MMP-13 expression was especially abundant in vulvar carcinomas showing metastasis to lymph nodes and was associated with expression of membrane type 1 MMP by tumor cells and gelatinase-A (MMP-2) by stromal cells, as detected by immunohistochemistry. MMP-13 mRNAs were detected in 9 of 11 cell lines established from vulvar carcinomas and in 4 of 6 cell lines from cervical carcinomas, whereas endometrial (n = 10) and ovarian (n = 9) carcinoma cell lines were negative for MMP-13 mRNA. No correlation was detected between MMP-13 expression and p53 gene mutations in vulvar SCC cell lines. However, MMP-13 expression was detected in 5 of 6 vulvar and cervical SCC cell lines harboring HPV 16 or 68 DNA. These results show that MMP-13 is specifically expressed by malignantly transformed squamous epithelial cells, including vulvar SCC cells, and appears to serve as a marker for their invasive capacity.  (+info)

Oxidized low-density lipoprotein regulates matrix metalloproteinase-9 and its tissue inhibitor in human monocyte-derived macrophages. (7/1761)

BACKGROUND: Macrophages in human atherosclerotic plaques produce a family of matrix metalloproteinases (MMPs), which may influence vascular remodeling and plaque disruption. Because oxidized LDL (ox-LDL) is implicated in many proatherogenic events, we hypothesized that ox-LDL would regulate expression of MMP-9 and tissue inhibitor of metalloproteinase-1 (TIMP-1) in monocyte-derived macrophages. MWRHOSA AND RESULTS: Mononuclear cells were isolated from normal human subjects with Ficoll-Paque density gradient centrifugation, and adherent cells were allowed to differentiate into macrophages during 7 days of culture in plastic dishes. On day 7, by use of serum-free medium, the macrophages were incubated with various concentrations of native LDL (n-LDL) and copper-oxidized LDL. Exposure to ox-LDL (10 to 50 microg/mL) increased MMP-9 mRNA expression as analyzed by Northern blot, protein expression as measured by ELISA and Western blot, and gelatinolytic activity as determined by zymography. The increase in MMP-9 expression was associated with increased nuclear binding of transcription factor NF-kappaB and AP-1 complex on electromobility shift assay. In contrast, ox-LDL (10 to 50 microg/mL) decreased TIMP-1 expression. Ox-LDL-induced increase in MMP-9 expression was abrogated by HDL (100 microg/mL). n-LDL had no significant effect on MMP-9 or TIMP-1 expression. CONCLUSIONS: These data demonstrate that unlike n-LDL, ox-LDL upregulates MMP-9 expression while reducing TIMP-1 expression in monocyte-derived macrophages. Furthermore, HDL abrogates ox-LDL-induced MMP-9 expression. Thus, ox-LDL may contribute to macrophage-mediated matrix breakdown in the atherosclerotic plaques, thereby predisposing them to plaque disruption and/or vascular remodeling.  (+info)

The matrix metalloproteinase-9 regulates the insulin-like growth factor-triggered autocrine response in DU-145 carcinoma cells. (8/1761)

The androgen-independent human prostate adenocarcinoma cell line DU-145 proliferates in serum-free medium and produces insulin-like growth factors (IGF)-I, IGF-II, and the IGF type-1 receptor (IGF-1R). They also secrete three IGF-binding proteins (IGFBP), IGFBP-2, -3, and -4. Of these, immunoblot analysis revealed selective proteolysis of IGFBP-3, yielding fragments of 31 and 19 kDa. By using an anti-IGF-I-specific monoclonal antibody (mAb), we detect surface receptor-bound IGF-I on serum-starved DU-145 cells, which activates IGF-1R and triggers a mitogenic signal. Incubation of DU-145 cells with blocking anti-IGF-I, anti-IGF-II, or anti-IGF-I plus anti-IGF-II mAb does not, however, inhibit serum-free growth of DU-145. Conversely, anti-IGF-1R mAb and IGFBP-3 inhibit DNA synthesis. IGFBP-3 also modifies the DU-145 cell cycle, decreases p34(cdc2) levels, and IGF-1R autophosphorylation. The antiproliferative IGFBP-3 activity is not IGF-independent, since des-(1-3)IGF-I, which does not bind to IGFBP-3, reverses its inhibitory effect. DU-145 also secretes the matrix metalloproteinase (MMP)-9, which can be detected in both a soluble and a membrane-bound form. Matrix metalloproteinase inhibitors, but not serpins, abrogate DNA synthesis in DU-145 associated with the blocking of IGFBP-3 proteolysis. Overexpression of an antisense cDNA for MMP-9 inhibits 80% of DU-145 cell proliferation that can be reversed by IGF-I in a dose-dependent manner. Inhibition of MMP-9 expression is also associated with a decrease in IGFBP-3 proteolysis and with reduced signaling through the IGF-1R. Our data indicate an IGF autocrine loop operating in DU-145 cells, specifically modulated by IGFBP-3, whose activity may in turn be regulated by IGFBP-3 proteases such as MMP-9.  (+info)

Table of Contents. Table of Contents 2. List of Tables 5. List of Figures 5. Introduction 6. Global Markets Direct Report Coverage 6. Matrix Metalloproteinase 9 (Gelatinase B or 92 kDa Type IV Collagenase or 92 kDa Gelatinase or MMP9 or EC 3.4.24.35) Overview 7. Therapeutics Development 8. Matrix Metalloproteinase 9 (Gelatinase B or 92 kDa Type IV Collagenase or 92 kDa Gelatinase or MMP9 or EC 3.4.24.35)-Products under Development by Stage of Development 8. Matrix Metalloproteinase 9 (Gelatinase B or 92 kDa Type IV Collagenase or 92 kDa Gelatinase or MMP9 or EC 3.4.24.35)-Products under Development by Therapy Area 9. Matrix Metalloproteinase 9 (Gelatinase B or 92 kDa Type IV Collagenase or 92 kDa Gelatinase or MMP9 or EC 3.4.24.35)-Products under Development by Indication 10. Matrix Metalloproteinase 9 (Gelatinase B or 92 kDa Type IV Collagenase or 92 kDa Gelatinase or MMP9 or EC 3.4.24.35)-Pipeline Products Glance 12. Late Stage Products 12. Early Stage Products 13. Matrix Metalloproteinase 9 ...
Purpose: Collagenase could be considered as a method for generating animal model of keratoconus. The authors aimed to evaluate the impact of collagenase on the interlamellar cohesive force of rabbit corneas.. Methods: 20 post mortem New Zealand white rabbit corneas were divided into 4 groups: group 1(15 mg/ml collagenase type II with 15% dextran, N=5), group 2(10 mg/ml collagenase type II with 15% dextran, N=5),group 3(5 mg/ml collagenase type II with 15% dextran, N=5) and group 4(the control group,15% dextran, N=5). After removing epithelium and measuring the corneal thickness, 9*4mm corneal strips were incised and interlamellar cohesive force at 50% depth of stroma was measured with a microcomputer-controlled biomaterial testing machine.. Results: The mean interlamellar cohesive force of group 1,group 2,group 3 and group 4 was 0.225 N/mm,0.217 N/mm,0.199 N/mm,and 0.211 N/mm respectively; without statistically significant differences. Light microscopy showed stromal tissue became less tight ...
BACKGROUND: 72 Kilodalton (kd) type IV collagenase is a matrix metalloproteinase that specifically cleaves type IV collagen molecules. The enzyme has been postulated to have an important role in the invasion and spread of malignant tumors. EXPERIMENTAL DESIGN: In situ hybridization was used to study the expression of the 72 kd type IV collagenase mRNA in 24 benign, 2 semimalignant, and 15 malignant ovarian tumors and in 5 metastases of ovarian serous adenocarcinomas. The results were correlated with the expression of the mRNA for the alpha 1(IV) chain of type IV collagen and with the corresponding immunohistochemical distribution of the enzyme. RESULTS: The results showed that the more malignant an ovarian tumor was, the more clearly mRNA expressions for both 72 kd type IV collagenase and the alpha 1(IV) chain could be detected in tumor cells. The expression of both types of mRNAs was localized within the cells of tumor stroma and occurred mainly in fibroblasts and vascular endothelial cells. ...
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Metalloproteinases that degrade extracellular matrix molecules play important roles in development and progression of various diseases. Among them, collagenases are unique as they have an ability to degrade triple helical interstitial collagens into 3/4 and 1/4 fragments, a crucial step for collagenolysis in the tissue. Collagenases, consisting of a catalytic domain and a hemopexin domain, requires both domains for collagenolysis. The enzymes unwind triple helical collagen before they hydrolyze the peptide bonds. Aggrecanases are also multidomain metalloproteinases belonging to the ADAMTS family, and the noncatalytic ancillary domains also play an important role in recognition of aggrecan and their activities. Attenuation of collagenase and aggrecanase activities will be achieved by inhibitors or antibodies that interact directly with those noncatalytic ancillary domains (exosite inhibitors). Such molecules will be attractive for therapy as they will be highly selective because they are based on the
TY - JOUR. T1 - Antitumor effect of pingyangmycin in combination with monoclonal antibody 3G11 directed against type IV collagenase. AU - Liu, Xiu Jun. AU - Ouyang, Zhi Gang. AU - Dai, Yao. AU - Liu, Xiao Yun. AU - Zhen, Yong Su. PY - 2007/2. Y1 - 2007/2. N2 - Objective: To study the antitumor effects of the combination of pingyangmycin (PYM) and monoclonal antibody(mAb) 3G11 directed against type IV collagenase. Methods: Immunoreactivity of mAb 3G11 to type IV collagenase and various tumor cells was determined by ELISA and the cytotoxicity of PYM and PYM plus 3G11 was examined by MTT assay. Antitumor effects in vivo were evaluated by using subcutaneously transplanted hepatoma 22 tumor model in mice. Results: mAb 3G11 showed immunoreactivity to type IV collagenase, mouse hepatoma 22 (H22) cells, human hepatoma HepG2 cells, and human prostatic carcinoma DU145 cells. As compared with free PYM, PYM plus 3G11 showed stronger cytotoxicity to these tumor cells. Synergetic effect was found at a certain ...
72 kDa Type IV Collagenase (Matrix Metalloproteinase 2 or Gelatinase A or Neutrophil Gelatinase or 72 kDa Gelatinase or TBE 1 or MMP2 or EC 3.4.24.24) - Pipeline Review, H2 2017 with 46 pages available at USD 3500 for single User PDF at ReportsWeb research database.
We previously advanced the hypothesis that a highly regulated balance of synthesis and degradation determines collagen content in the fibrous cap of atherosclerotic plaques.1,2 In turn, collagen levels critically influence the integrity of the plaques cap, a structure whose biomechanical failure may cause most myocardial infarctions. Earlier indirect evidence suggested that collagenases of the MMP family can regulate collagen content in the plaque.5-12 We initially demonstrated overexpression of the prototypical interstitial collagenase MMP-1 in human atheromata5 and later showed colocalization of MMP-1/collagenase-1 and MMP-13/collagenase-3 with degraded collagen in these lesions as detected by an antibody specific for the collagenase cleavage site of collagen.9 Recently, our group showed that human atheromata contain a third interstitial collagenase, MMP-8/collagenase-2,11 also present in mouse atheromata, as shown here (Figure 2C). Shah et al32 reported that conditioned media of cultured ...
1FLS: High-resolution solution structure of the catalytic fragment of human collagenase-3 (MMP-13) complexed with a hydroxamic acid inhibitor.
1FM1: High-resolution solution structure of the catalytic fragment of human collagenase-3 (MMP-13) complexed with a hydroxamic acid inhibitor.
Using quantitative zymography, we measured activity of the type IV collagenases metalloprotease 2 (MMP-2) and MMP-9 in 192 biopsies from colorectal carcinomas, adenomas, and normal bowel. The median level of MMP-9 in samples from Dukes stage A (n = 18) or C (n = 48) tumors was significantly higher than in stage B carcinomas (n = 65), adenomas (n = 25), and normals (n = 36; P = 0.0001). The median level of active MMP-2 was significantly higher in stage A or C compared with adenomas (P = 0.0001) and normals (P = 0.0001). The median level of inactive MMP-2 was higher in all Dukes stages compared with normals and adenomas (P = 0.0001). There was a significant increase in inactive MMP-2 from Jass prognostic groups I-IV (P = 0.006) but no correlation with the active enzyme. MMP activity was not related to tumor differentiation, colon versus rectal location, or disease-free, 5-year survival. All groups expressed mRNA for both enzymes, but there were quantitative and locational differences in MMP-2 ...
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Worthington Collagenase Vial, NCIS [WLK003245-5VI] - UNSPSC Code(s):12352204≥500 units per mg dry weightA component of the NCIS kit. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 5 ml of HBSS or equivalent yields a solution of 300 units/ml of collagenase, Code: CLSPA. Suitable for cell
This enzyme is synthesized as a proenzyme of 53 kDa that is converted to an active form of 22 kDa. cDNA sequences have been obtained for the mouse [3] and human [4] enzymes. In peptidase family M10 (interstitial collagenase family ...
Neutrophil collagenase (EC 3.4.24.34, matrix metalloproteinase 8, PMNL collagenase, MMP-8) is an enzyme. This enzyme catalyses the following chemical reaction Cleavage of interstitial collagens in the triple helical domain. Unlike EC 3.4.24.7, interstitial collagenase, this enzyme cleaves type III collagen more slowly than type I This enzyme belongs to the peptidase family M10. Collagenase Hasty, K. (1987). "A., Jeffrey, J. J., Hibbs, M. S. and Welgus, H. G. The collagen substrate specificity of human neutrophil collagenase". J. Biol. Chem. 262 (21): 10048-10052. PMID 3038863. Hasty, K. (1990). "A., Pourmotabbed, T. F., Goldberg, G. I., Thompson, J. P., Spinella, D. G., Stevens, R. M. and Mainardi, C. L. Human Neutrophil Collagenase. A distinct gene product with homology to other matrix metalloproteinases". J. Biol. Chem. 265 (20): 11421-11424. PMID 2164002. Knäuper, V.; Krämer, S.; Reinke, H.; Tschesche, H. (1990). "Characterization and activation of procollagenase from human ...
We studied the in vivo effect of long-term doxycycline treatment combined with NSAID on human interstitial collagenases, other matrix metalloproteinases, serine proteinases, tissue inhibitor of matrix metalloproteinase-l (TIMP-1) and lactoferrin from saliva and serum during the course of acute reactive arthritis (ReA). Collagenase activity and serine proteases (elastase-like, cathepsin G-like and trypsin-like activities) of saliva (n = 10) and gelatinase, lactoferrin and TIMP-1 of saliva (E = 10) and serum (n = 10) samples before and after 2 months doxycycline treatment, combined with NSAID, were studied by quantitative SDS-PAGE assay, ELISA assay and by spectrophotometric assay. The cellular source and molecular forms of salivary collagenase were characterized by immunoblotting using specific antisera. We found that activities of total and endogenously active interstitial collagenase reduced significantly. The salivary collagenase was found to originate from neutrophils. No fragmentation of ...
The mechanisms involved in retinoic acid (RA)-mediated regulation of the collagenase gene in a rabbit synovial fibroblast cell line (HIG82) were investigated. When HIG82 cells are cotransfected with expression vectors containing cDNAs for retinoic acid receptor (RAR) α1, β2, or γ1 and collagenase promoter-driven CAT reporter constructs, only RAR-γ1 represses basal CAT expression upon RA treatment, while RAR-α1, β2, and γ1 all suppress phorbol-induced CAT expression. Thus, transcriptional regulation of collagenase by RA is mediated by RARs in an RAR-type specific manner. Using mutatlonal and deletional analysis, we find that interaction between elements within 182 bp collagenase promoter plays an important role in this process. In addition, cotreatment with RA results in a decrease of phorbol-induced mRNA levels of fos and jun, and binding of nuclear proteins to an AP-1 oligonucleotide. Furthermore, RA-induced nuclear protein(s) specifically bind to a 22 bp sequence (−182 to −161) of the
The early stages of degradation of native collagen by two bacterial collagenases were studied by electron microscopy and by automatic Edman degradation. The purified collagenase from Clostridium histolyticum was shown to cleave native collagen at several sites, but not progressively from the N-terminus, as had been previously suggested. The homogeneous collagenase from Achromobacter iophagus cleaves native collagen preferentially at two sites corresponding to the interbands 33-34 and 41-42. The latter lies within the region cleaved by the eukaryotic collagenases. ...
Reagent Set-up:. Solution A To make one liter: Add to 700 ml of DI water 71.48 gm of Hepes, 10 ml of Phenol Red (1.75mg/ml), Sodium Hydroxide to bring to pH of 7.0, 7.2 gm of glucose, 2.24 gm of potassium chloride, 71.28 gm of sodium chloride, and 1.42 gm of sodium phosphate. pH again to 7.5 then add 300 ml of DI water to complete volume to 1 liter. Sterile filter (22 micron filter) and keep in 4C.. Digestion enzyme stocks Make 10x stocks of collagenase type 1 and 100x stocks of dispase. For collagenase type 1, add 13,659 U to 9.1 ml of X media (final concentration= 1500U/ml). For dispase, add 5mg to 1.25 ml of X media (final concentration= 4mg/ml). Stocks can be stored for up to 2 weeks in -20C.. 1x digestion mix Combine together 3.0 ml of 10x collagenase, 300 μl of 100x dispase, 750 μl of fetal bovine serum, and approximately 26 ml of X media to make 30 mls of digestion mix. Final concentrations of enzymes in 1x digestion mix: collagenase= 150 U/ml, dispase= 40 μg/ml; final percentage of ...
Our most active crude collagenase, type XI, contains significant activities of other proteolytic enzymes, including clostripain, caseinase, and a tryptic activity. While this product is highly suited for preparation of pancreatic islets and islet cells, the tryptic activity of the collagenase medium can activate the zymogens of the digestive enzymes produced by pancreatic acinar cells. The addition of trypsin inhibitor to the culture medium from Clostridium histolyticum prevents or greatly reduces the activation of the protease proenzymes stored in the pancreas.
Autotransplantation of cryopreserved ovarian cortex can be associated with a risk of cancer cell reseeding. This issue could be eliminated by grafting isolated preantral follicles. Collagenase NB6 is an enzyme produced under good manufacturing practices (GMP) in compliance with requirements for tissue engineering and transplantation in humans and thus can be used to isolate preantral follicles from ovarian tissue in the framework of further clinical applications. Multicolor flow cytometry is an effective tool to evaluate the potential contamination of follicular suspensions by leukemic cells. The efficiency of collagenase NB6 was evaluated in comparison to collagenase type IA and Liberase DH, in terms of yield, morphology and viability. A short-term in vitro culture of follicles isolated with collagenase NB6 was conducted for 3 days in a fibrin matrix. A modelization procedure was carried out to detect the presence of leukemic cells in follicular suspensions using multicolor flow cytometry (MFC). No
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72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that in humans is encoded by the MMP2 gene. The MMP2 gene is located on chromosome 16 at position 12.2. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix (ECM) in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Activation of MMP-2 requires proteolytic processing. A complex of membrane type 1 MMP (MT1-MMP/MMP14) and tissue inhibitor of metalloproteinase 2 recruits pro-MMP 2 from ...
Chemical and Biological Reagents. CVT-3619 (Lot number 736-87) was synthesized by the Department of Medicinal and Bio-Organic Chemistry of CV Therapeutics, Inc. (Palo Alto, CA). Other agents were purchased from the following sources: collagenase types I and IV from Worthington Biochemicals (Lakewood, NJ); adenosine deaminase (ADA) and protease inhibitor cocktail tablets (Complete) from Roche Diagnostics (Mannheim, Germany); [3H]CPX from PerkinElmer Life and Analytical (Boston, MA); [3H]ZM241385 from Tocris-Cookson (Bristol, UK); [3H]MRE3008F20 from GE Healthcare (Little Chalfont, Buckinghamshire, UK); DDT1MF-2, CHO, and HEK cell lines, F-12K medium, and fetal bovine serum from ATCC (Manassas, VA); trypsin-EDTA from Clonetics (Baltimore, MD); and G418 from U.S. Biological (Swampscott, MA). All other chemicals were purchased from Sigma-Aldrich (St Louis, MO). Adenosine receptor agonists and antagonists were dissolved in dimethyl sulfoxide as 10 mM stock solutions and diluted in physiological ...
Dupuytrens is a condition that affects the hands and fingers, which causes the fingers to bend into the palm. It is thought to be more genetically linked but diabetes, smoking and some medications can also cause this condition. It is a condition that is non-cancerous, and non-life threatening. Current research does not indicate there is any link between Dupuytrens Contracture and Metastatic melanoma. Treatments can be non surgical and surgical and doctors, as a rule, do not like treating unless it is affecting full functioning of the hand. Initial non evasive treatment which may be of help can include massaging the affected area and exercise/physiotherapy to assist with blood flow to the area and help loosen the contracture.. Non surgical treatments include radiation therapy, which is done to the nodes and cords. The way this works is to interfere with the growth of the fibroblasts which cause the nodules. Another non surgical treatment is collagenase injections that break down the ...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.
Worthington Collagenase, Type 5 [WLS005284-1MG] - UNSPSC Code(s):12352204≥450 units per mg dry weightPrepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
CDw75, 100 Tests. CD75 is a 43-85 kD type II transmembrane sialoprotein, known as lacosamines, or CDw75. It is a member of glycosyltransferase family.
Protease capable of digesting collagen fibrils. Contains high levels of collagenase and caseinase activity. Produced using animal component-free materials.
MATRIX DEGRADING PROTEINASES FROM HUMAN-GRANULOCYTES - TYPE-I, TYPE-II, TYPE-III COLLAGENASE, GELATINASE AND TYPE-IV, TYPE-V-COLLAGENASE - A SURVEY OF RECENT FINDINGS AND INHIBITION BY GAMMA-ANTICOLLAGENASE ...
We examined the effects of IGF 1 around the collagenolytic action of DU145 cells utilizing gelatin MAPK 阻害剤 zymography that is an incredibly delicate technique
TY - JOUR. T1 - A one-step sandwich enzyme immunoassay for inactive precursor and complexed forms of human matrix metalloproteinase 9 (92 kDa gelatinase/type IV collagenase, gelatinase B) using monoclonal antibodies. AU - Fujimoto, Noboru. AU - Hosokawa, Nobuko. AU - Iwata, Kazushi. AU - Shinya, Takashi. AU - Okada, Yasunori. AU - Hayakawa, Taro. PY - 1994/11. Y1 - 1994/11. KW - Matrix metalloproteinase 9. KW - Monoclonal antibody. KW - Sandwich enzyme immunoassay. KW - Tissue inhibitor of metalloproteinases 1. UR - http://www.scopus.com/inward/record.url?scp=0027971387&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027971387&partnerID=8YFLogxK. U2 - 10.1016/0009-8981(94)90256-9. DO - 10.1016/0009-8981(94)90256-9. M3 - Article. C2 - 7704951. AN - SCOPUS:0027971387. VL - 231. SP - 79. EP - 88. JO - Clinica Chimica Acta. JF - Clinica Chimica Acta. SN - 0009-8981. IS - 1. ER - ...
TY - JOUR. T1 - Thermo-sensitive assembly of the biomaterial REP reduces hematoma volume following collagenase-induced intracerebral hemorrhage in rats. AU - Park, Joohyun. AU - Kim, Jong Youl. AU - Choi, Seong Kyoon. AU - Kim, Jae Young. AU - Kim, Jae Hwan. AU - Jeon, Won Bae. AU - Lee, Jong Eun. PY - 2017/8. Y1 - 2017/8. N2 - Intracerebral hemorrhage (ICH) frequently results in severe disabilities and high mortality. RGD-containing elastin-like polypeptide (REP), a modified elastin-like polypeptide (ELP), is a thermally responsive biopolymer. REP has high affinity for cells and is known to show non-immunotoxicity, -cytotoxicity, and -inflammatory responses. Here we found that administration of REP in the acute phase of the ICH rat model reduced the hematoma volume, decreased the number of activated microglia, attenuated the expression of von Willebrand Factor (vWF), and prevented the leakage of immunoglobulin G (IgG) into the cerebral parenchyma without any occlusion of intact microvessels. ...
EliKine™ Human MMP-9 ELISA Kit employs a two-site sandwich ELISA to quantitate Human MMP-9.,MMP 9; MMP-9; MMP9; Matrix Metalloproteinase 9; GELB; Type V collagenase; Matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase),Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
Some of the MMPs seem to be expressed in all or almost all traumatic synovial membrane samples. Traumatic synovial membrane is naturally not normal synovial membrane. However, this may imply a constitutive expression pattern and a role in normal tissue remodelling processes. These MMPs were MMP-2 (gelatinase A or 72 kDa type IV collagenase), MMP-3 (stromelysin-1), MMP-11 (stromelysin-3) and MMP-19. Their constitutive expression does not mean that they could not play a part in pathological tissue destruction, for example, because of their increased synthesis in diseases and/or insufficient regulation of their degradative potential. For example, MMP-2 has recently been found to be part of a cell membrane associated ternary MT1-MMP/TIMP-2/MMP-2 complex, which may contribute to proMMP-2 activation and focused, pericellular targeting of its action.20 In addition, MMP-2 is, not only gelatinase, but also a collagenase active against interstitial type I and II collagens.21 22 Some MMP mRNAs were found ...
Auxilium Pharmaceutical, manufacturer of Xiaflex collagenase injections, boasts that in a two-year study Xiaflex had a recurrence rate of 19.3 percent, a considerably lower rate of return than those who used surgical procedures to treat their hand problem.. The answer to the question "Is the Xiaflex recurrence rate for Dupuytren low?" is a qualified yes and no.. Yes, Xiaflex or collagenase treatment results in a considerably lower Dupuytren recurrence rate than hand surgery. However, this does not necessarily mean that the rate of recurrence is actually low; it only means the problem will come back slower than what happens after invasive hand surgery. The reality is that hand surgery has a tremendously high recurrence rate, so a non-surgical therapy option by comparison will look favorable.. Every child learns that pointing to someone who has done something worse than you does not diminish his crime. When you told your mother that the child next door stole 25 cents from his mother, you were ...
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An extracellular endopeptidase of vertebrate tissues homologous with interstitial collagenase. Digests proteoglycan, fibronectin, collagen types III, IV, V, IX, and activates procollagenase. In peptidase family M10 (interstitial collagenase family ...
FIQH HADIS; SOLAT SUNAT ISYRAQ - *SOLAT SUNAT ISYRAQ* Sabda Nabi SAW; مَنْ صَلَّى الغَدَاةَ فِي جَمَاعَةٍ ثُمَّ قَعَدَ يَذْكُرُ اللَّهَ حَتَّى تَطْلُعَ الشَّمْسُ، ثُمَّ صَلَّى رَكْعَتَيْن... ...
XIAFLEX (Collagenase clostridium histolyticum) drug information & product resources from MPR including dosage information, educational materials, & patient assistance.
Learn about Xiaflex (Collagenase Clostridium Histolyticum) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, reviews, and related medications.
We conducted a retrospective audit of hospital data to calculate the cost of surgical fasciectomy. Costings were calculated internally in collaboration with finance and compliance staff using the Medicare benefits schedule (MBS) code 46372 to identify patients who underwent fasciectomy over a 12-month period from March 2014 to April 2015. Only cost calculations for single-digit fasciectomy were included in order to make this cohort comparable to the CCH treatment group. The CCH cost was calculated from participants in a prospective study from June 2014 to March 2016. The total cost for fasciectomy procedure included all hospital and operating theatre-related expenses such as: medical and surgical costs, operating theatre costs, anaesthetic staff costs, allied health costs, blood products, medical imaging, pathology, pharmacy, prosthesis costs and ward costs. All costs included direct costs and related overheads. Actual figures were obtained from the hospital cost centre in our billing department ...
Collagenases of the matrix metalloproteinase (MMP) family play major roles in morphogenesis, tissue repair, and human diseases, but how they recognize and cleave the collagen triple helix is not fully understood. Here, we report temperature-dependent binding of a catalytically inactive MMP-1 mutant (E200A) to collagen through the cooperative action of its catalytic and hemopexin domains. Contact between the two molecules was mapped by screening the Collagen Toolkit peptide library and by hydrogen/deuterium exchange. The crystal structure of MMP-1(E200A) bound to a triple-helical collagen peptide revealed extensive interactions of the 115-Å-long triple helix with both MMP-1 domains. An exosite in the hemopexin domain, which binds the leucine 10 residues C-terminal to the scissile bond, is critical for collagenolysis and represents a unique target for inhibitor development. The scissile bond is not correctly positioned for hydrolysis in the crystallized complex. A productive binding mode is readily
Using SANTYL COLLAGENASE OINTMENT 250UNITS/GM during pregnancy may raise the risk of children developing some disorder (commpon for some such kind of drugs), however it depends upon how SANTYL COLLAGENASE OINTMENT 250UNITS/GM ingredients pass through placenta and may have effect on baby - Strength of SANTYL COLLAGENASE OINTMENT 250UNITS/GM is major factor in determination of such side effects, The possible danger in pregnancy are under research. BIOMED, S.L. Canada publish leaflet about SANTYL COLLAGENASE OINTMENT 250UNITS/GM every update to describe possible risks of using SANTYL COLLAGENASE OINTMENT 250UNITS/GM side effect in pregnancy and pregnant women. You may download BIOMED, S.L. issued leaflet regarding side effects of SANTYL COLLAGENASE OINTMENT 250UNITS/GM - COLLAGENASE. Pregnancy Side Effects can be easily know by Atc code of SANTYL COLLAGENASE OINTMENT 250UNITS/GM ATC CODE.. ...
Mice. C57BL/6 AireGW/+ mice (17) and C57BL/6 AireGW/+ TRP-1 TCR Tg RAG−/− mice (11) were housed and bred in sterile, specific pathogen-free mouse facilities at the University of North Carolina at Chapel Hill and UCSF. C57BL/6 Aire WT littermate controls were used in all experiments. RAG1−/− mice were purchased from the Jackson Laboratory.. Antibodies and flow cytometry. Anti-CD4 (clone RM4-5), anti-CD3 (clone 145-2c11), anti-Ki67 (clone SolA15), anti-KLRG1 (clone 2F1), anti- granzyme B (clone NGZB), anti-FOXP3 (clone FJK-16s), and anti-CD25 (clone PC61.5) antibodies were purchased from eBioscience. Anti-CD8a (clone 5H10) antibody was purchased from Invitrogen. Intracellular staining for cytokines and FOXP3 were performed as in ref. 11. All samples were run on a Dako CyAn flow cytometer (Beckman-Coulter) and analyzed using FlowJo (TreeStar Inc.).. Isolation of TILs. TILs were isolated as previously described (11). Tumors were dissected and minced before incubation with collagenase type ...
Full-length recombinant human neutrophil pro-collagenase (MMP-8), latent form. Matrix metalloproteinase 8 (MMP-8), or neutrophil collagenase, degrades interstitial collag
T-lymphocyte migration into tissues requires focal degradation of the basement membrane. In this study, we show that transient adherence to fibronectin induces
Increased inflammation delays wound healing in mice deficient in collagenase-2 (MMP-8).: Matrix metalloproteinases (MMPs) have been implicated in numerous tissu
The irreversible destruction of the tissues that comprise synovial joints is the hallmark of both rheumatoid arthritis and osteoarthritis. In both diseases, inflammatory cytokines, such as interleukin-1β, stimulate the production of matrix metalloproteinases (MMPs), a family of enzymes that collectively degrade all components of the extra-cellular matrix. The collagenases, a subgroup of the MMP family, have the unique ability to cleave the collagen fibrils that comprise cartilage, tendon, and bone, and thereby provide the rate-limiting step in the degradation of many joint structures. In the arthritides, MMP-1 and MMP-13 (collagenase-1 and collagenase-3, respectively) are key mediators of joint destruction, and therefore represent potential therapeutic targets. In this thesis, we identified the rexinoid LG100268 (LG268), a ligand for the nuclear hormone receptor (NHR) RXR, as a novel, selective inhibitor of MMP-1 and -13 expression, and investigated the molecular mechanisms behind its ...
Cell Culture. The murine macrophage cell line J774A.1 was grown in RPMI 1640 medium (Invitrogen, Carlsbad, CA) supplemented with 100 U/ml penicillin, 100 μg/ml streptomycin, 50 μg/ml gentamicin, 20 U/ml polymyxin B, and 10% fetal bovine serum. Alternatively, peritoneal macrophages were isolated 5 days after injection of 2 ml of Brewers thioglycolate medium (Sigma-Aldrich, St. Louis, MO) into the peritoneal cavity of C56BL/6 mice as reported previously (McCarron et al., 1984). Primary macrophages were added to culture flasks and allowed to adhere for 2 h at 37°C. Nonadherent cells were removed by three washes of warm medium. Adherent cells were ,99% macrophages as assessed by immunocytochemical detection of the macrophage marker F4/80 (anti-F4/80, clone Cl:A3-1; Serotec, Oxford, UK). Smooth muscle cells were isolated from mouse or rabbit aorta by collagenase type 2 (Worthington, Lakewood, NJ) and elastase (Sigma-Aldrich) digestion (60-90 min at 37°C) at 300 and 5 U/ml final concentration, ...
Selective modulation of α7 nicotinic acetylcholine receptors (nAChRs) is thought to regulate processes impaired in schizophrenia, Alzheimers disease, and other dementias. One approach to target α7 nAChRs is by positive allosteric modulation. Structurally diverse compounds, including PNU-120596, 4-naphthalene-1-yl-3a,4,5,9b-tetrahydro-3-H-cyclopenta[c]quinoline-8-sulfonic acid amide (TQS), and 5-hydroxyindole (5-HI) have been identified as positive allosteric modulators (PAMs), but their receptor interactions and pharmacological profiles remain to be fully elucidated. In this study, we investigated interactions of these compounds at human α7 nAChRs, expressed in Xenopus laevis oocytes, along with genistein, a tyrosine kinase inhibitor. Genistein was found to function as a PAM. Two types of PAM profiles were observed. 5-HI and genistein predominantly affected the apparent peak current (type I) whereas PNU-120596 and TQS increased the apparent peak current and evoked a distinct weakly decaying ...
The pathological destruction of collagen plays a key role in the development of inflammatory disease states affecting every organ system in the human body. Neutrophils localized at inflammatory sites can potentially degrade collagen by releasing a metalloenzyme, collagenase, which is stored in a latent inactive form. Triggered human neutrophils were shown to release and simultaneously activate their latent collagenase. The activation of the latent enzyme was coupled to an oxidative process that required the generation of a highly reactive oxygen metabolite, hypochlorous acid. Oxidative regulation of latent collagenase activity may be important in the pathogenesis of connective tissue damage in vivo.. ...
The primary aim of this thesis was to quantify the collagenase concentrations in carotid plaques and to relate them to markers to plaque instability.;Recent studies have shown that strain therapy decreases cardiovascular risk, even in patients with normal cholesterol levels. A further aim of this thesis was to observe the effects of statins on clinical and biochemical indicators of plaque instability.;Atherosclerotic plaques were collected from 159 patients undergoing carotid endarterectomy. The presence and timing of carotid territory symptoms was ascertained. Pre-operative embolisation was recorded by transcranial Doppler. Each plaque was assessed for histological features of instability. Plaque MMP and cytokine concentrations was quantified using ELISA.;Significantly higher concentrations of active MMP-8 were observed in the plaques of symptomatic patients (p=0.0002), emboli-positive patients (p=0.0037) and in those plaques demonstrating histological evidence of rupture (p=0.0036). No ...
The human tissue inhibitor of metalloproteinases (TIMP) is a glycoprotein with a molecular weight of 28,000. It appears to be ubiquitous in human mesoderm tissues and has previously been shown to be identical to the collagenase inhibitor isolated from human skin fibroblasts. TIMP inhibits type I- and IV-specific collagenases and other neutral metalloen-doproteinases that may be responsible for the degradation of extracellular matrix in tumor cell metastasis. In this work we have utilized recombinant human TIMP (rTIMP) obtained by expression of its cDNA gene (Carmichael et al., Proc. Natl. Acad. Sci. USA, 83: 2407, 1986). The rTIMP is shown to have similar inhibition properties as natural TIMP against human skin fibroblast collagenase. In an in vitro amnion invasion assay system, rTIMP inhibited the invasion of B16-F10 murine melanoma cells through the human amniotic membrane at an identical concentration to that reported previously for natural TIMP. The mechanism by which rTIMP inhibits amniotic ...
Assay Kits , MMP Assay Kits , Human MMP-7, Recombinant; Matrix metalloproteinases (MMP s) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components. This recombinant human MMP-7, expressed as the pro-enzyme, becomes highly active when incubated with APMA. It can be used in FRET-based enzyme activity assay or served as positive control for Western blot, IP, and ELISA.
Matrix Metalloproteinase 2: A secreted endopeptidase homologous with INTERSTITIAL COLLAGENASE, but which possesses an additional fibronectin-like domain.
WASHINGTON -- The FDA has approved the first drug for the progressive hand disease known as Dupuytrens contracture -- the injectable collagenase clostridium histolyticum (Xiaflex).
Hi all, The results of the stool test last month showed that catalase, urease and gelatinase are positive. This explains my inflammation and leaky...
Tissue inhibitor of metalloproteinases (TIMP)-2 forms a noncovalent complex with the precursor of matrix metalloproteinase 2 (proMMP-2, progelatinase A) through interaction of the C-terminal domain of each molecule. We have isolated the proMMP-2-TIMP-2 complex from the medium of human uterine cervical fibroblasts and investigated the processes involved in its activation by 4-aminophenylmercuric acetate (APMA). The treatment of the complex with APMA-activated proMMP-2 by disrupting the Cys73-Zn2+ interaction of the zymogen. This is triggered by perturbation of the proMMP-2 molecule, but not by the reaction of the SH group of Cys73 with APMA. The activated proMMP-2 (proMMP-2*) formed a new complex with TIMP-2 by binding to the N-terminal inhibitory domain of the inhibitor without processing the propeptide. Thus the APMA-treated proMMP-2*-TIMP-2 complex exhibited no gelatinolytic activity. In the presence of a small amount of free MMP-2, however, proMMP-2* in the complex was converted into the 65 kDa MMP
Gelatinase B (MMP-9) and galectin-3 are widely known to participate in tumor cell invasion and metastasis. Glycans derived from MMP-9 expressed in MCF-7 breast cancer and THP-1 myeloid leukemia cells were compared with those from MMP-9 expressed in natural neutrophils. The many O-linked glycans of neutrophil gelatinase B presented a cluster of mainly galactosylated core II structures, 46% of which were ligands for galectin-3; 11% contained two to three N-acetyllactosamine repeating units that are high-affinity ligands for the lectin. The glycan epitopes thus provide MMP-9 with both high-affinity and (presumably) high-avidity interactions with galectin-3. In contrast, the O-glycans released from MMP-9 expressed in MCF-7 and THP-1 cells were predominantly sialylated core I structures. Only 10% of MCF-7 and THP-1 gelatinase B O-glycans were ligands for galectin-3 and contained only a maximum single N-acetyllactosamine repeat. Consistent with the glycan analysis, surface plasmon resonance binding ...
Invitrogen™ eBioscience™ Human MMP-9 Platinum ELISA Kit 96 tests Invitrogen™ eBioscience™ Human MMP-9 Platinum ELISA Kit L-M ELISA Kits
Since the first description of matrix metalloproteinase (MMP)-1 as an interstitial collagenase, great importance has been ascribed to this enzyme in extracellular matrix remodeling during tumoral, inflammatory, and angiogenic processes. As more evidence for the role of MMPs in targeting nonmatrix substrates emerges, casual observations that intracellular MMP-1 is found in vitro and in vivo prompt investigation of the role that MMP-1 may play on basic cell functions such as cell division and apoptosis. Here we show for the first time that MMP-1 not only has extracellular functions but that it is strongly associated with mitochondria and nuclei and accumulates within the cells during the mitotlc phase of the cell cycle. On induction of apoptosis, MMP-1 co-localized with aggregated mitochondria and accumulated around fragmented nuclei. Inhibition of this enzyme by RNA interference or treatment with a broad MMP inhibitor caused faster degradation of lamin A, activation of caspases, and fragmentation ...
This study establishes the expression of stromelysin-3 in advanced human atheroma and furthermore provides evidence for (a) colocalization of this matrix metalloproteinase with CD40 on lesional EC, SMC, and MØ in situ and (b) regulation of de novo expression of stromelysin-3 by CD40 ligation, rather than by the classical soluble mediators of MMPs such as IL-1, TNF-α, or IFN-γ. We also demonstrated in vivo that the interruption of CD40- CD40L interaction markedly reduced the expression of stromelysin-3 in mouse atheroma. The finding that EC, SMC, and MØ express stromelysin-3 establishes atheroma-associated cells as novel sources of stromelysin-3. Thus, this report provides evidence that stromelysin-3, whose expression correlates with the invasiveness of malignancies ((3), (11), (12), (16), (17), (54)), might also participate in the pathogenesis of another common human disease, atherosclerosis. In view of its unusual substrate specificity, stromelysin-3 might participate in several pathways. ...
Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes
Vranka, Janice A., "The characterization of gelatinase inhibition and the involvement of the matrix metalloproteinases and their inhibitors in glaucoma and a retinal degeneration" (1997). Scholar Archive. 2639 ...
H-ras transformed human bronchial epithelial cells (TBE-1) secrete a single major extracellular matrix metalloprotease which is not found in the normal parental cells. The enzyme is secreted in a latent form which can be activated to catalyze the cleavage of the basement membrane macromolecule type IV collagen. The substrates in their order of preference are: gelatin, type IV collagen, type V collagen, fibronectin, and type VII collagen; but the enzyme does not cleave the interstitial collagens or laminin. This protease is identical to gelatinase isolated from normal human skin explants, normal human skin fibroblasts, and SV40-transformed human lung fibroblasts. Based on this ability to initiate the degradation of type IV collagen in a pepsin-resistant portion of the molecule, it will be referred to as type IV collagenase. This enzyme is most likely the human analog of type IV collagenase detected in several rodent tumors. Type IV collagenase consists of three domains. Type IV collagenase ...
This study demonstrates that in human carotid arteries, the matrix-degrading enzyme MMP-1 is expressed in advanced atherosclerotic plaques but not in nonatherosclerotic intima. This enzyme, which degrades types I and III collagen, could contribute to plaque expansion, disruption, and thrombosis. The source of MMP-1 is a population of vascular wall cells, in particular a subset of plaque macrophages. In vitro, macrophages, SMCs, and endothelial cells have been shown to release a number of matrix-degrading proteases, including MMP-1.11 12 13 Macrophage-derived foam cells and also some SMCs in atherosclerotic plaques have been shown to express stromelysin (MMP-3),14 which degrades principally proteoglycan core protein but not types I and III collagen.27 MMP-1 would be required for the degradation of these two matrix proteins,11 and it has been shown that fibrous caps from unstable plaques have collagen fiber disruption.16 The present study shows that MMP-1 mRNA and protein are expressed primarily ...
Evidence-Based Complementary and Alternative Medicine (eCAM) is an international peer-reviewed, Open Access journal that seeks to understand the sources and to encourage rigorous research in this new, yet ancient world of complementary and alternative medicine.
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
Emeto T, Seto SW, Golledge J. Targets for medical therapy to limit abdominal aortic aneurysm progress. Curr Drug Targets (2014) 15(9): 860-873. Wang Y, Emeto T, Lee J, Marshman L, Moran C, Seto SW, Golledge J. Mouse modes of intracranial aneurysm. Brain Pathology (2014) Accepted Manuscript.. Seto SW, Krishna SM, Moran CS, Liu D, Golledge J. Aliskiren limits abdominal aortic aneurysm, ventricular hypertrophy and atherosclerosis in an apolipoprotein E deficient mouse model. Clin Sci (Lond) (2014) 127(2): 123-34.. Wang Y, Seto SW, Golledge J. Angiotensin II, sympathetic nerve activity and chronic heart failure. Heart Failure Reviews (2014) 19(2): 187-198.. 2013. Clancy P, Seto SW, Koblar SA, Golledge J. Role of angiotensin converting enzyme 1/angiotensin II/ angiotensin receptor 1 axis in interstitial collagenase expression in human carotid atheroma. Atherosclerosis (2013) 229(2): 331-337.. Seto SW, Au AL, Poon CC, Zhang Q, Li RW, Yeung JH, Kong SK, Ngai SM, Wan S, Ho HP, Lee SM, Hoi MP, Chan SW, ...
Looking for online definition of Gelatinases in the Medical Dictionary? Gelatinases explanation free. What is Gelatinases? Meaning of Gelatinases medical term. What does Gelatinases mean?
BACKGROUND: Dupuytrens disease of the hand is a common condition affecting the palmar fascia, resulting in progressive flexion deformities of the digits and hence limitation of hand function. The optimal treatment remains unclear as outcomes studies have used a variety of measures for assessment. METHODS: A literature search was performed for all publications describing surgical treatment, percutaneous needle aponeurotomy or collagenase injection for primary or recurrent Dupuytrens disease where outcomes had been monitored using functional measures. RESULTS: Ninety-one studies met the inclusion criteria. Twenty-two studies reported outcomes using patient reported outcome measures (PROMs) ranging from validated questionnaires to self-reported measures for return to work and self-rated disability. The Disability of Arm, Shoulder and Hand (DASH) score was the most utilised patient-reported function measure (n=11). Patient satisfaction was reported by eighteen studies but no single method was used
in Journal of Cell Science (1991), 100((Pt 3)), 649-55. The Hutchinson-Gilford syndrome (progeria) is a rare disorder in childhood characterized by premature and accelerated aging. This study reports the effect of a potent growth factor, EGF, on the ... [more ▼]. The Hutchinson-Gilford syndrome (progeria) is a rare disorder in childhood characterized by premature and accelerated aging. This study reports the effect of a potent growth factor, EGF, on the proliferative capacities and extracellular matrix macromolecules and collagenase expression of two strains of progeria skin-derived cells. At low population doubling levels (PDL less than 10), confluent cultures of progeria fibroblasts made quiescent by lowering the concentration of serum in the medium did not respond to EGF while the mitotic activity of normal PDL-matched fibroblasts was almost maximally restored upon addition of EGF. No obvious difference between normal and low PDL progeria fibroblasts was observed in the number and in the ...
BioAssay record AID 208335 submitted by ChEMBL: Tested for inhibition of stromelysin using [3H]transferrin, following pre-incubation with prostromelysin for 0 hr prior to activation by plasmin.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Effect of topical application of the collagenase IV inhibitor, SB-3CT, on hair regrowth.Notes: After 5, 15, and 25 days treatment, typical photos of dorsal skin
Santyl Collagenase is an enzymatic debrider. CLAIMED BENEFITS: It actively and selectively removes necrotic tissue without harming granulation tissue. INTENDED USE: indicated for ...
The Secondary Outcome Measure for participants who were enrolled at Step1 through Step2 and treated with AK160 is the time to first achieve and maintain clinical success after the last injection where clinical success was defined as reduction in the contracture of the first treated joint to 5° or less. The injection was allowed up to 3 times ...
Gage R&R in Excel is easy and youll really understand gage R&R maths if you follow the example... or just download the add-in for quick reliable results
15/02/2018: The recruitment end date has been updated from 31/01/2018 to 02/08/2017. The overall trial end date has been updated from 01/07/2019 to 01/02/2019. The intention to publish date has been added. 18/01/2016: Added publication reference ...
Lung PMN content in the vasculature and the lung interstitium was determined by a recently developed method to distinguish between PMNs in both compartments.20 Briefly, 5 min before death, a fluorochrome-labeled antibody (anti GR-1) to murine PMNs was injected intravenously, resulting in a complete labeling of all intravascular PMNs without accessing PMNs in the lung tissue. Anti-mouse GR-1 antibody was purified from supernatant of the GR-1 hybridoma (ATCC) by the biomolecular facility of University of Virginia (Charlottesville, VA). GR-1 was labeled with a staining kit following the manufacturers protocol (Alexa Fluor 633; Molecular Probes, Carlsbad, CA). Nonadherent PMNs were removed by flushing the pulmonary vasculature through the spontaneously beating right ventricle with 10 ml PBS at a pressure of 25 cm H2O. BAL was performed as described above to remove alveolar PMN. Then, lungs were minced and digested with 125 U/ml collagenase type XI, 60 U/ml hyaluronidase type I-s, and 60 U/ml DNAse1 ...
The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-2 (also designated type IV collagenase) cleaves Collagen Types IV, V, VII and X and gelatin type I. Activation of MMP-2 secretion requires the Ras signaling pathway ...
TIMP-3 (Tissue inhibitor of metalloproteinases 3) is a secreted extracellular matrix protein, metalloprotease inhibitor. It forms complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. TIMP-3 is critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. Defects in TIMP-3 are the cause of Sorsby fundus dystrophy (SFD). SFD is a rare autosomal dominant macular disorder with an age of onset in the fourth decade. It is characterized by loss of central vision from subretinal neovascularization and atrophy of the ocular tissues. Generally, macular disciform degeneration develops in the patients eye within 6 months to 6 years ...
Background: Effective digestive enzymes are crucial for successful islet isolation. Supplemental proteases are essential because they synergize with collagenase for effective pancreatic digestion. The activity of these enzymes is critically dependent on the presence of Ca2+ ions at a concentration of 5-10 mM. The present study aimed to determine the Ca2+ concentration during human islet isolation and to ascertain whether the addition of supplementary Ca2+ is required to maintain an optimal Ca2+ concentration during the various phases of the islet isolation process.. Methods: Human islets were isolated according to standard methods and isolation parameters. Islet quality control and the number of isolations fulfilling standard transplantation criteria were evaluated. Ca2+ was determined by using standard clinical chemistry routines. Islet isolation was performed with or without addition of supplementary Ca2+ to reach a Ca2+ of 5 mM.. Results: Ca2+ concentration was markedly reduced in ...
The incidence of malignant melanoma has steadily increased from a risk of 1 in 250 in 1980 to a projection of 1 in 90 by 2000 (20) . Despite early diagnosis, surgery, and chemotherapy, cure rates have not been improved, and death rates continue to rise (20 , 21) . Doxorubicin is not commonly used as a therapeutic agent for malignant melanoma. However, in animal studies, doxorubicin in combination with the chemopreventive agent NAC (22 , 23) decreased tumor formation and metastasis in a murine model of melanoma, an effect attributed, at least in part, to the selective inhibition of gelatinase A and gelatinase B by NAC (24) . In addition, doxorubicin-induced inhibition of melanoma cell invasion correlated with decreases in tumor cell motility and increases in focal contact formation in the mouse melanoma cell line K1735-M2 (25) . Thus, there is precedence for examining the effect of this drug on collagenase 1 gene expression. In this study, we found that low concentrations of doxorubicin, i.e., ...
BioAssay record AID 712141 submitted by ChEMBL: Prodrug conversion assessed as recombinant human MMP-12 mediated compound conversion to(S)-5-((S)-1-amino-1-oxopropan-2-ylamino)-4-((S)-2-((S)-2-(2-(biphenyl-4-ylsulfonamido)acetamido)-4-methylpentanamido)-4-carboxybutanamido)-5-oxopentanoic acid by HPLC analysis.
Ultra-LEAF™ Purified anti-human CD11c Antibody - CD11c is a 145-150 kD type I transmembrane glycoprotein also known as integrin αX and CR4.
The EliKine™ Human MMP-9 ELISA Kit is also known as MMP-9 or GELB ELISA Kit, which is the latest product released by Abbkine Scientific. The company has launched the product as a part of its plan to revolutionize the field of life science and scientific research. This product is designed specifically to help scientific research workers to solve professional difficulties and accelerate the pace of scientific research.. Matrix metallopeptidase 9 (MMP-9) may play an important role in angiogenesis and neovascularization. For example, MMP9 appears to be involved in the remodeling associated with malignant glioma neovascularization. Also, MMP9 has been found to be associated with numerous pathological processes, including cancer, placental malaria, immunologic and cardiovascular diseases.. The MMP-9 Human ELISA Kit is the new addition to EliKine™ series of ELISA kits family. The featured kit includes Human MMP-9 microplate, Human MMP-9 standard, Human MMP-9 detect antibody, EliKine™ ...
[119 Pages Report] Check for Discount on Global (North America, Europe and Asia-Pacific, South America, Middle East and Africa) Collagenase Market 2017 Forecast to 2022 report by Global Info Research. Collagenase, obtained from Clostridium histolyticum, is an enzyme used for...
Since MMP activity is also regulated by TIMP binding (Nagase and Woessner, 1999) and the dissociation of TIMP-MMP complexes during gel electrophoresis prior to zymography assays acts to enhance the apparent activity of proMMP isoforms, soluble gelatinase activity in the cell‐conditioned media samples was assayed using a peptide substrate (Figure 6C). Both v‐Src3T3 and v‐Src FRNK S‐1034 cells contained high levels of soluble gelatinase activity, whereas NIH‐3T3 and the various v‐Src FRNK cell clones had ∼4‐fold lower levels of gelatinase activity secreted from the same number of cells (Figure 6C). Analysis of whole‐cell lysates also revealed that FRNK expression resulted in lower levels of cell‐associated gelatinase activity (Figure 6D). Although blotting analyses did not reveal significant changes in TIMP expression (data not shown), addition of recombinant TIMP‐2 to v‐Src3T3s inhibited Matrigel invasion activity in a dose‐dependent manner (Figure 6E). Taken together, ...
Objective-To investigate the efficacy and safety of a low-volume, single-catheter, continuous peripheral neural blockade (CPNB) technique to locally deliver bupivacaine to alleviate signs of severe forelimb pain resulting from experimentally induced tendonitis in horses. Design-Randomized controlled experimental trial. Sample-14 horses and 5 forelimbs from equine cadavers. Procedures-Horses underwent collagenase-induced superficial digital flexor tendonitis in the midmetacarpal region of 1 forelimb. ...
Mouse anti Human MMP-9 Activated antibody, clone 4A3 recognizes the active form of human matrix metalloproteinase 9 (MMP-9). The MMPs are
Plasmin-induced tissue degradation contributes to the proliferation of most diseases. Of particular interest is the fact that similar mechanisms are induced by attacking pathogens as they are used by the defending host cells, e.g. macrophages. In many pathological conditions macrophages become activated. This activation reflects a particular state of alert that is characterized by an abundant release of secretory products. These products include oxygen metabolites, collagenases, elastases, and a significantly increased secretion of plasminogen activators. It is immediately obvious that this mechanism needs to be precisely controlled. Therefore macrophages also secrete inhibitory products including plasmin inhibitors and a2-macroglobulin which are able to inactivate plasmin and many other proteases. Any imbalance in this control system leads to an exacerbation of this mechanism and to continued tissue degradation. Chronic activation of macrophages and an exertion of the control mechanisms ...
MMP-2, MMP-9 and their inhibitors TIMP-2 and TIMP-1 production by human monocytes in vitro in the presence of different forms of hydroxyapatite particles.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Pacific Blue™ anti-human CD304 (Neuropilin-1) Antibody - CD304, also known as neuropilin-1, BDCA-4 and VEGF165R, is a 140 kD type I transmembrane protein.
Collagenase Bacteriocins Immunosuppressive proteins Bone resorption agents Inhibitors of bone formation Antibiotic resistance ...
... collagenases such as cathepsin B1; and hyaluronidase. PSGAG inhibits the synthesis of prostaglandin E2, which is released upon ...
Neutrophil collagenase, also known as matrix metalloproteinase-8 (MMP-8) or PMNL collagenase (MNL-CL), is a collagen cleaving ... 1991). "Characterization of 58-kilodalton human neutrophil collagenase: comparison with human fibroblast collagenase". ... 1995). "Neutrophil collagenase (MMP-8) cleaves at the aggrecanase site E373-A374 in the interglobular domain of cartilage ... 1990). "Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases". J. Biol. Chem ...
Matrix metalloproteinase-1 (MMP-1) also known as interstitial collagenase and fibroblast collagenase is an enzyme that in ... MMP-1 was the first vertebrate collagenase both purified to homogeneity as a protein, and cloned as a cDNA. MMP-1 has an ... Dumin JA, Dickeson SK, Stricker TP, Bhattacharyya-Pakrasi M, Roby JD, Santoro SA, Parks WC (August 2001). "Pro-collagenase-1 ( ... Goldberg GI, Wilhelm SM, Kronberger A, Bauer EA, Grant GA, Eisen AZ (May 1986). "Human fibroblast collagenase. Complete primary ...
Collagenase No. 14 is present in MeSH but not listed as a collagenase, while No. 18 is absent from MeSH. The main substrates of ... These groups are the collagenases, the gelatinases, the stromelysins, and the membrane-type MMPs (MT-MMPs). The collagenases ... The collagenases are No. 1, #8, No. 13, and No. 18. In addition, No. 14 has also been shown to cleave fibrillar collagen, and ... Eisen A, Jeffrey J, Gross J (1968). "Human skin collagenase. Isolation and mechanism of attack on the collagen molecule". ...
These collagenases break down protein into peptides. The peptides are subsequently reduced to their constituent amino acids, ... The first step in the process involves the elimination of the organic collagen fraction by the action of bacterial collagenases ...
Examples are hyaluronidase and collagenase. These molecules, however, are enzymes that are secreted by a variety of organisms ...
Bode W (June 1995). "A helping hand for collagenases: the haemopexin-like domain". Structure. 3 (6): 527-30. doi:10.1016/s0969- ...
"A helping hand for collagenases: the haemopexin-like domain". Structure. 3 (6): 527-30. doi:10.1016/s0969-2126(01)00185-x. PMID ...
Examples of such metalloproteinases are collagenases, gelatinases and stromelysins. These collagenases digest Type-I collagen, ...
Sottrup-Jensen L, Birkedal-Hansen H (1989). "Human fibroblast collagenase-alpha-macroglobulin interactions. Localization of ...
In recent decades, collagenase has begun to find various practical medical applications. The Ines Mandl Medical Foundation of ... Ines Mandl, Collagenase (Gordon and Breach 1972). "Ines Mandl". Legacy. Retrieved 11 August 2016. "Pioneering Biochemist's NYU- ... In 1950, she became the first scientist to extract collagenase from the bacterium Clostridium histolyticum. Other work involved ... was an Austrian-born American biochemist who was awarded the Garvan-Olin Medal in 1983 for her work on the enzyme collagenase. ...
Kabadjova, P.; Vlahov, S. (15 April 2014). "Regulation of Extracellular Collagenase Production in 91". Biotechnology & ...
The treatment with collagenase is different for the MCP joint and the PIP joint. In a MCP joint contracture the needle must be ... Collagenase injection is most effective at "Stage I" and Stage II" of 6-90 degrees of deformation. However, it is also used at ... The collagenase is distributed across three injection points. For the PIP joint the needle must be placed not more than 4 mm ... Clostridial collagenase injections have been found to be more effective than placebo. The cords are weakened through the ...
This bacterium synthesizes collagenase in periodontal disease. Prevotella Parte, A.C. "Bacteroides". www.bacterio.net. 93. ...
Complete loss of the stroma can occur within 24 hours.[12] Treatment includes antibiotics and collagenase inhibitors such as ... Contains macroglobulins with anti-collagenase effects.. A combination of the above may be necessary early in the disease course ... These infectious agents produce proteases and collagenases which break down the corneal stroma. ...
95 kDa type IV collagenase/gelatinase; gelatinase B, Type V collagenase, collagenase IV; collagenase type IV; gelatinase MMP 9 ... Metaloproteinase matriks-9 (bahasa Inggris: 92-kDa gelatinase; matrix metalloproteinase 9; type V collagenase; 92-kDa type IV ...
72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that in humans is ... Hrabec E, Naduk J, Strek M, Hrabec Z (2007). "[Type IV collagenases (MMP-2 and MMP-9) and their substrates--intracellular ... Identity with type IV collagenase from HT1080 cells". J. Biol. Chem. 267 (35): 25228-32. PMID 1460022. "MMP2 gene". Genetics ... "Entrez Gene: MMP2 matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)". Martignetti JA, Aqeel ...
2000). "Matrix metalloproteinases collagenase-2, macrophage elastase, collagenase-3, and membrane type 1-matrix ... Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. It is a member of the matrix metalloproteinase (MMP) ... 1999). "Collagenase-3 binds to a specific receptor and requires the low density lipoprotein receptor-related protein for ... 1997). "The helping hand of collagenase-3 (MMP-13): 2.7 A crystal structure of its C-terminal haemopexin-like domain". J. Mol. ...
... K is the most potent mammalian collagenase. Cathepsin K is involved in osteoporosis, a disease in which a decrease in ...
Chordee Hypospadias Collagenase clostridium histolyticum Freedberg, Irwin M.; Fitzpatrick, Thomas B. (2003). Fitzpatrick's ... Some consider the use of nonsurgical approaches to be "controversial". Collagenase clostridium histolyticum (marketed as ...
... is made of hyaluronic acid and lubricin, proteinases, and collagenases. Synovial fluid exhibits non-Newtonian ...
Collagenases are zinc metalloproteases that cleave collagen and gelatin into small fragments. The seven collagenases are alpha ... This collagenase has been used to treat Dupuytren's contracture, a disease of pathological collagen production and deposition ... Collagenase clostridium histolyticum is manufactured and marketed by Auxilium Pharmaceuticals in the US, and marketed by SOBI ... Collagenase clostridium histolyticum is secreted by the bacterium and can destroy connective tissue of muscles. ...
H. (1973). "A Collagenase in extracts of the invertebrate Bipalium kewense". Biochemical Journal. 133: 329-334. doi:10.1042/ ...
Wu H, Wu T, Xu X, Wang J, Wang J (May 2011). "Iron toxicity in mice with collagenase-induced intracerebral hemorrhage". J Cereb ...
"Iron toxicity in mice with collagenase-induced intracerebral hemorrhage". Journal of Cerebral Blood Flow & Metabolism. 31 (5): ...
Collagenase Clostridium Histolyticum) may treat, uses, dosage, side effects, drug interactions, warnings, patient labeling, ... consisting of two microbial collagenases in a defined mass ratio, Collagenase AUX-I and Collagenase AUX-II, which are isolated ... are allergic to collagenase clostridium histolyticum, or any of the ingredients in XIAFLEX, or to any other collagenase product ... are allergic to collagenase clostridium histolyticum or any of the ingredients in XIAFLEX, or to any other collagenase product ...
Collagenase clostridium histolyticum) drug information & product resources from MPR including dosage information, educational ... Allergy to collagenase used in other therapeutic applications. Warnings/Precautions:. Should be administered by person ... Allergy to collagenase used in other therapeutic applications. Warnings/Precautions:. Should be administered by person ... Collagenase clostridium histolyticum 0.9mg; per vial; lyophilized pwd for reconstitution with supplied diluent; for ...
Collagenase clostridium histolyticum. Medicine used in less severe cases. Its injected into the hand to weaken the collagen ...
This pilot study evaluates the safety and tolerability of a single injection of collagenase enzyme directly into a uterine ... An Open Label, Dose Escalation Study to Assess Safety and Tolerability of Collagenase Clostridium Histolyticum (EN3835) in ... An Open Label, Dose Escalation Study to Assess Safety and Tolerability of Collagenase Clostridium Histolyticum (EN3835) in ...
Collagenase is injected into the cord and is left for 24 hours after which the finger can then be straightened (by the surgeon ... Injection with collagenase. This is a relatively new treatment for dupuytrens contracture which achieves, by chemical means, ... Both needle fasciotomy and collagenase injection need careful selection since not all cases will be suitable. Those most ...
Percutaneous needle fasciotomy and collagenase injections are significant therapeutic alternatives to surgery. ...
... one collagenase vial) and (2) the collagenase acting under the skin over the following 36 hours. All four locations (RH index ... I am a 57 year old male in Perth, Australia and have just had collagenase injections in both hands (5 days ago). The painful ... I believe the needle approach has to be too exacting to be successful while the collagenase approach is very general (it ... Recent studies using collagenase injections have been promising (Journal of Hand Surgery, July-Aug 2007). ...
Collagenase injection as nonsurgical treatment of Dupuytrens disease: 8-year follow-up". J Hand Surg Am. vol. 35. 2010. pp. ... "Injectable collagenase clostridium histolyticum for Dupuytrens contracture". N Engl J Med. vol. 361. 2009. pp. 968-79. ... Of 1,080 collagenase treated joints, 35% experienced recurrence at 3 years, with recurrence more likely at the PIP than MCP ... There was no evidence of recurrence in collagenase-treated individuals at 90-day follow-up in CORD I. However, 3-year follow-up ...
Collagenase Injection: An enzymatic drug that breaks down collagen can be injected into the corded tissue to soften and weaken ...
Yes, Xiaflex or collagenase treatment results in a considerably lower Dupuytren recurrence rate than hand surgery. However, ... Auxilium Pharmaceutical, manufacturer of Xiaflex collagenase injections, boasts that in a two-year study Xiaflex had a ...
On Day 1, a small injection of collagenase is released into and left to loosed the stiff portion of the cord. On Day 2, the ...
With this collagenase in the lymph nodes of the arm pit it dissolved most of the tissues of the region and even destroyed the ... I guess it all comes down to how difficult it is to get that collagenase enzyme ONLY into the abnormal and fibrous tissue of ... I am concerned about your last comment: "my other fingers are not the same." Perhaps the Xiaflex collagenase enzyme that was ... collagenase). When I first started to hear this pattern develop I thought it is almost as though Xiaflex somehow migrates to ...
Collagenase Clostridium Histolyticum Injection: learn about side effects, dosage, special precautions, and more on MedlinePlus ... tell your doctor and pharmacist if you are allergic to collagenase Clostridium histolyticum injection, collagenase ointment ( ... Collagenase Clostridium histolyticum injection comes as a powder to be mixed with a liquid and injected by a doctor. If you are ... Collagenase Clostridium Histolyticum Injection. pronounced as (kol a jen ase) (klos trid ee um) (his toe lit ik um) ...
Microbial collagenases have been identified from bacteria of both the Vibrio and Clostridium genera. Collagenase is used during ... Collagenases may be used for tenderizing meat in a manner similar to widely used tenderizers papain, bromelain and ficain. ... Collagenases are enzymes that break the peptide bonds in collagen. They assist in destroying extracellular structures in the ... In addition to being produced by some bacteria, collagenase can be made by the body as part of its normal immune response. This ...
Easy-to-read patient leaflet for Collagenase (Topical). Includes indications, proper use, special instructions, precautions, ... Collagenase (Topical). Generic Name: Collagenase (Topical) (KOL la je nase). Brand Name: Santyl ... What do I need to tell my doctor BEFORE I take Collagenase?. *If you have an allergy to collagenase or any other part of ... How is this medicine (Collagenase) best taken?. Use collagenase (topical) as ordered by your doctor. Read all information given ...
... the tryptic activity of the collagenase medium can activate the zymogens of the digestive enzymes produced by pancreatic acinar ... Our most active crude collagenase, type XI, contains significant activities of other proteolytic enzymes, including clostripain ... USA Home > Product Directory > Biochemicals and Reagents > Enzymes, Inhibitors, and Substrates > Collagenases > Crude ... Collagenase + protease inhibitor 2-5 FALGPA units/mg solid, ≥800 CDU/mg solid, Suitable for isolation of rat pancreatic islet ...
Neutrophil collagenase (EC 3.4.24.34, matrix metalloproteinase 8, PMNL collagenase, MMP-8) is an enzyme. This enzyme catalyses ... Collagenase Hasty, K. (1987). "A., Jeffrey, J. J., Hibbs, M. S. and Welgus, H. G. The collagen substrate specificity of human ... Neutrophil collagenase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and Cellular Biology ... Unlike EC 3.4.24.7, interstitial collagenase, this enzyme cleaves type III collagen more slowly than type I This enzyme belongs ...
Collagenase clostridium histolyticum is used to treat Dupuytrens contracture in adults. This condition causes an abnormal ... Collagenase clostridium histolyticum is made from a mixture of proteins derived from a certain bacteria. ... What is collagenase clostridium histolyticum?. Collagenase clostridium histolyticum is made from a mixture of proteins derived ... What is the most important information I should know about collagenase clostridium histolyticum?. Collagenase clostridium ...
Reportstack has announced a new market research reports namely United States Collagenase Industry 2015 and Europe Collagenase ... The Collagenase market analysis is provided for the United States and Europe markets including development trends, competitive ... The Collagenase industry development trends and marketing channels are analyzed. Finally the feasibility of new investment ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
View drug interactions between collagenase clostridium histolyticum and Januvia. These medicines may also interact with certain ... collagenase clostridium histolyticum. A total of 93 drugs are known to interact with collagenase clostridium histolyticum. ... Collagenase clostridium histolyticum is in the drug class miscellaneous uncategorized agents.. *Collagenase clostridium ... There were no interactions found in our database between collagenase clostridium histolyticum and Januvia - however, this does ...
Evaluation of a collagenase generated osteoarthritis biomarker in the synovial fluid from elbow joints of dogs with medial ... METHODS: Concentrations of a collagenase-generated cleavage neoepitope of type II collagen (Col2-3/4C(long mono), or C2C) in ...
... collagenase-1), MMP-8 (collagenase-2), and MMP-13 (collagenase-3). A secondary cleavage followed the initial cleavage produced ... These data suggest that collagenase(s) produced by chondrocytes is (are) involved in the cleavage and denaturation of type II ... We demonstrate the direct involvement of increased collagenase activity in the cleavage of type II collagen in osteoarthritic ... Enhanced cleavage of type II collagen by collagenases in osteoarthritic articular cartilage.. ...
Prior to US Food and Drug Administration approval of injectable collagenase, the standard of care was surgical fasciectomy, ... Phase 2 studies which confirmed the optimal dose of collagenase as 10,000 units (0.58 mg) showed injectable collagenase reduced ... Starkweather KD, Lattuga S, Hurst LC, Badalamente MA, Guilak F, Sampson S, Dowd A, Wisch D. Collagenase in the treatment of ... Injectable collagenase Clostridium histolyticum represents a novel, nonsurgical approach to the treatment of Dupuytren ...
Borden P, Solymar D, Sucharczuk A, Lindman B, Cannon P, Heller RA: Cytokine control of interstitial collagenase and collagenase ... Gomis-Ruth FX, Gohlke U, Betz M, Knauper V, Murphy G, Lopez-Otin C, Bode W: The helping hand of collagenase-3 (MMP-13): 2.7 A ... Johansson N, Saarialho-Kere U, Airola K, Herva R, Nissinen L, Westermarck J, Vuorio E, Heino J, Kahari VM: Collagenase-3 (MMP- ... Stura EA, Visse R, Cuniasse P, Dive V, Nagase H: Crystal structure of full-length human collagenase 3 (MMP-13) with peptides in ...
  • The present study used mice with a targeted mutation in both alleles of Col1a1 (Col1a1 tml Jae or Col R/R ) that yields amino acid substitutions around the collagenase cleavage site in the α1(I) chains that render collagen completely resistant to attack by MMP collagenases. (ahajournals.org)
  • However, the collagen-like genes we have been able to test so far (vkg and CG42453) did not appear to be associated with the filaments, demonstrating that the collagenase-sensitive component of the filaments is one of a number of other Drosophila proteins bearing a collagenase cleavage site. (biomedsearch.com)
  • A targeted mutation at the known collagenase cleavage site in mouse type I collagen impairs tissue remodeling. (rupress.org)
  • AUX-I and AUX-II have been shown by substrate activity patterns to be representative of the Class I (ColG) and Class II (ColH) collagenases, respectively, and differ in preferred cleavage site on the collagen molecule. (gms-books.de)
  • Collagenase is a protease consisting of a single polypeptide chain approximately 1,000 amino acid residues in length. (stemcell.com)
  • As supplied, this product is stable for one year at -20°C. There is no loss in FALGPA or protease activity in 30 days at 37°C, 50°C and -20°C. Solutions of crude collagenase are stable if frozen quickly in aliquots (at 10 mg/mL) and kept frozen at -20°C. Further freeze-thaw cycles will damage the solution. (sigmaaldrich.com)
  • Crude preparation that contains several collagenases, a sulfhydryl protease, clostripain, and trypsin-like proteolytic activities. (emdmillipore.com)
  • Upregulation of gelatinases A and B, collagenases 1 and 2, and increased parenchymal cell death in COPD. (nih.gov)
  • Immunohistochemical analysis of COPD lungs showed a markedly increased expression of collagenases 1 and 2, and gelatinases A and B, while collagenase 3 was not found. (nih.gov)
  • These findings suggest that there is an upregulation of collagenase 1 and 2 and gelatinases A and B, and an increase in endothelial and epithelial cell death, which may contribute to the pathogenesis of COPD through the remodeling of airways and alveolar structures. (nih.gov)
  • Clinical results from phase 3 studies confirmed the efficacy and safety of injectable collagenase as a viable nonsurgical intervention for the treatment of patients with Dupuytren contracture, translating the observations made in the laboratory into the clinical setting. (springer.com)
  • Efficacy and safety of injectable mixed collagenase subtypes in the treatment of Dupuytren's contracture. (springer.com)
  • That year, vertebrate collagenase was discovered in bullfrog tadpole tissue, followed by a vast number of collagenases in other samples of bacteria, amphibians, marine life, and mammals. (agscientific.com)
  • Signal transduction through the fibronectin receptor induces collagenase and stromelysin gene expression. (rupress.org)
  • Monoclonal antibodies to the FnR that block initial adhesion of fibroblasts to fibronectin induced the expression of genes encoding the secreted extracellular matrix-degrading metalloproteinases collagenase and stromelysin. (rupress.org)
  • That induction was a direct consequence of interaction with the FnR was shown by the accumulation of mRNA for stromelysin and collagenase. (rupress.org)
  • However, adhesion to covalently immobilized peptides containing the arg-gly-asp sequence that were derived from fibronectin, varying in size from hexapeptides up to 120 kD, induced collagenase and stromelysin gene expression. (rupress.org)
  • Willmroth F, Peter HH, Conca W: A matrix metalloproteinase gene expressed in human T lymphocytes is identical with collagenase 3 from breast carcinomas. (drugbank.ca)
  • At noncytotoxic concentrations, i.e. , concentrations lower than those normally used in cancer chemotherapy, the anthracycline doxorubicin specifically inhibited collagenase 1 (MMP-1) gene expression in the highly invasive and metastatic human melanoma cell line A2058. (aacrjournals.org)
  • Therefore, we were interested in whether genotoxic chemotherapy agents might be similarly able to preferentially alter the expression of the inducible collagenase 1 gene, thereby potentially altering tumor invasiveness. (aacrjournals.org)
  • The virR gene, a member of a class of two-component response regulators, regulates the production of perfringolysin O, collagenase, and hemagglutinin in Clostridium perfringens. (asm.org)
  • This fragment activated the transcription of the pfoA gene and also restored the production of collagenase (kappa-toxin) and hemagglutinin in strain SI112. (asm.org)
  • To better understand the role of collagenase-3 (MMP-13) in joint inflammation by investigating the consequences of transient overexpression of human collagenase-3 (matrix metalloproteinase-13 (MMP-13)), introduced by adenoviral gene delivery, in the mouse knee joint. (bmj.com)
  • We show here that mice carrying a collagenase-resistant mutant Col1a-1 transgene die late in embryo-genesis, ascribable to overexpression of the transgene, since the same mutation introduced into the endogenous Col1a-1 gene by gene targeting permitted normal development of mutant mice to young adulthood. (rupress.org)
  • Collagenases are categorized into 1 of 2 classes based on gene of origin, protein domain, and substrate specificity . (gms-books.de)
  • Although some studies suggest that MMPs may mediate SMC migration through the collagenous matrix of plaques, 15-19 the in vivo role of collagenases in this context remains unproven. (ahajournals.org)
  • Collagenase exposure does not decrease the interlamellar cohesive force in rabbit corneas, indicating that there is no significant effect of collagenase on interlamellar cohesion. (arvojournals.org)
  • Aortic atheromata of both groups had similar sizes and numbers of macrophages, a major source of collagenases. (ahajournals.org)
  • This is believed to be based on the fact that once collagen is degraded by collagenase , select inflammatory cells, including macrophages, are able to enhance migration and in turn, their efficiency. (agscientific.com)
  • Collagenases are proteinases that hydrolyze collagen in its native triple-helical conformation under physiological conditions, resulting in lysis of collagen deposits and, when injected into a Dupuytren's cord, in the enzymatic disruption of that cord . (gms-books.de)
  • These observations directly support the idea that synovial collagenase participates in cartilage erosion in rheumatoid arthritis. (nih.gov)
  • Thus the specificity of the collagenases for cartilage aggrecan was not influenced by the presence or absence of the C-terminal domain. (uni-bielefeld.de)
  • Prior to US Food and Drug Administration approval of injectable collagenase, the standard of care was surgical fasciectomy, which in many cases can result in suboptimal treatment outcomes and a high recurrence rate. (springer.com)
  • There is no information available on collagenase absorption through skin or its concentration in body fluids associated with therapeutic and/or toxic effects, degree of binding to plasma proteins, degree of uptake by a particular organ or in the fetus, and passage across the blood brain barrier. (bioportfolio.com)
  • CONCLUSION: We demonstrated that IL-17 and IL-1beta induced collagenase-3 production in OA chondrocytes mainly through AP-1 mediated transcriptional activity but with differential protein complexes, suggesting that some AP-1 proteins play a pivotal role in the different cytokine responses in terms of collagenase-3 production. (jrheum.org)
  • And now Collagenase free from animal proteins is available. (biophoretics.com)
  • Human peripheral blood lymphocytes incubated in culture for 1 to 3 days at 37 degree C, but not at 4 degree C, release a soluble factor which can stimulate, up to 400-fold, collagenase production by isolated, adherent, rheumatoid synovial cells. (sciencemag.org)
  • Autoregulation of collagenase production by a protein synthesized and secreted by synovial fibroblasts: cellular mechanism for control of collagen degradation. (uni-bielefeld.de)
  • However, one case of systemic manifestations of hypersensitivity to collagenase in a patient treated for more than one year with a combination of collagenase and cortisone has been reported. (bioportfolio.com)