Viruses whose host is Escherichia coli.
A family of bacteriophages that infects enterobacteria, CAULOBACTER, and PSEUDOMONAS. The genome consists of linear, positive-sense single-stranded RNA.
Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.
A family of rod-shaped or filamentous bacteriophages consisting of single-stranded DNA. There are two genera: INOVIRUS and PLECTROVIRUS.
Contamination of bodies of water (such as LAKES; RIVERS; SEAS; and GROUNDWATER.)
Refuse liquid or waste matter carried off by sewers.
The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.
The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.
A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.
Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.
A species of temperate bacteriophage in the genus P2-like viruses, family MYOVIRIDAE, which infects E. coli. It consists of linear double-stranded DNA with 19-base sticky ends.
A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".
The aggregation of suspended solids into larger clumps.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
Viruses whose hosts are bacterial cells.
The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.
A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.
A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.
A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.
Viruses whose genetic material is RNA.
The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment.
A commonly used laboratory solvent. It was previously used as an anesthetic, but was banned from use in the U.S. due to its suspected carcinogenicity.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.
The presence of bacteria, viruses, and fungi in the air. This term is not restricted to pathogenic organisms.
Water containing no significant amounts of salts, such as water from RIVERS and LAKES.
Viruses whose nucleic acid is DNA.
Means or process of supplying water (as for a community) usually including reservoirs, tunnels, and pipelines and often the watershed from which the water is ultimately drawn. (Webster, 3d ed)
Techniques used in microbiology.
Large natural streams of FRESH WATER formed by converging tributaries and which empty into a body of water (lake or ocean).
A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.
The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.
Process of growing viruses in live animals, plants, or cultured cells.
Deoxyribonucleic acid that makes up the genetic material of viruses.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
Process of determining and distinguishing species of bacteria or viruses based on antigens they share.
Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.
Divisions of the year according to some regularly recurrent phenomena usually astronomical or climatic. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Ribonucleic acid that makes up the genetic material of viruses.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The relationships of groups of organisms as reflected by their genetic makeup.
Warm-blooded VERTEBRATES possessing FEATHERS and belonging to the class Aves.
Proteins found in any species of virus.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Marker effects on reversion of T4rII mutants. (1/3698)

The frequencies of 2-aminopurine- and 5-bromouracil-induced A:T leads to G:C transitions were compared at nonsense sites throughout the rII region of bacteriophage T4. These frequencies are influenced both by adjacent base pairs within the nonsense codons and by extracodonic factors. Following 2AP treatment, they are high in amber (UAG) and lower in opal (UGA) codons than in allelic ochre (UAA) codons. In general, 5BU-induced transitions are more frequent in both amber and opal codons than in the allelic ochre codons. 2AP- and 5BU-induced transition frequencies in the first and third positions of opal codons are correlated with those in the corresponding positions of the allelic ochre codons. Similarly, the frequencies of 2AP-induced transition in the first and second positions of amber codons and their ochre alleles are correlated. However, there is little correlation between the frequencies of 5BU-induced transitions in the first and second positions of allelic amber and ochre codons.  (+info)

Bacteriophage inactivation at the air-water-solid interface in dynamic batch systems. (2/3698)

Bacteriophages have been widely used as surrogates for human enteric viruses in many studies on virus transport and fate. In this investigation, the fates of three bacteriophages, MS2, R17, and phiX174, were studied in a series of dynamic batch experiments. Both MS2 and R17 readily underwent inactivation in batch experiments where solutions of each phage were percolated through tubes packed with varying ratios of glass and Teflon beads. MS2 and R17 inactivation was the result of exposure to destructive forces at the dynamic air-water-solid interface. phiX174, however, did not undergo inactivation in similar studies, suggesting that this phage does not accumulate at air-water interfaces or is not affected by interfacial forces in the same manner. Other batch experiments showed that MS2 and R17 were increasingly inactivated during mixing in polypropylene tubes as the ionic strength of the solution was raised (phiX174 was not affected). By the addition of Tween 80 to suspensions of MS2 and R17, phage inactivation was prevented. Our data suggest that viral inactivation in simple dynamic batch experiments is dependent upon (i) the presence of a dynamic air-water-solid interface (where the solid is a hydrophobic surface), (ii) the ionic strength of the solution, (iii) the concentration of surface active compounds in the solution, and (iv) the type of virus used.  (+info)

End group of naturally terminated and UV lesion terminated T7 in vitro RNA. (3/3698)

The 3' terminal nucleosides of RNA transcribed in vitro by E. coli RNA polymerase from T7 DNA and UV irradiated TN DNA were determined. The 3' terminal nucleoside of naturally terminated (t1 termination site) RNA cytidine. In the case of RNA terminated at UV lesions, it is cytidine in 0 per cent of the molecules and adenosine in the remaining 30 per cent. Cytidine trialcohols are labile in high concentrations of KOH and at high temperature and appear to convert to uridine.  (+info)

Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage. (4/3698)

We report DNA and predicted protein sequence similarities, implying homology, among genes of double-stranded DNA (dsDNA) bacteriophages and prophages spanning a broad phylogenetic range of host bacteria. The sequence matches reported here establish genetic connections, not always direct, among the lambdoid phages of Escherichia coli, phage phiC31 of Streptomyces, phages of Mycobacterium, a previously unrecognized cryptic prophage, phiflu, in the Haemophilus influenzae genome, and two small prophage-like elements, phiRv1 and phiRv2, in the genome of Mycobacterium tuberculosis. The results imply that these phage genes, and very possibly all of the dsDNA tailed phages, share common ancestry. We propose a model for the genetic structure and dynamics of the global phage population in which all dsDNA phage genomes are mosaics with access, by horizontal exchange, to a large common genetic pool but in which access to the gene pool is not uniform for all phage.  (+info)

Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. (5/3698)

Lysogenic bacteriophages are major vehicles for the transfer of genetic information between bacteria, including pathogenicity and/or virulence determinants. In the enteric pathogen Escherichia coli O157:H7, which causes hemorrhagic colitis and hemolytic-uremic syndrome, Shiga toxins 1 and 2 (Stx1 and Stx2) are phage encoded. The sequence and analysis of the Stx2 phage 933W is presented here. We find evidence that the toxin genes are part of a late-phage transcript, suggesting that toxin production may be coupled with, if not dependent upon, phage release during lytic growth. Another phage gene, stk, encodes a product resembling eukaryotic serine/threonine protein kinases. Based on its position in the sequence, Stk may be produced by the prophage in the lysogenic state, and, like the YpkA protein of Yersinia species, it may interfere with the signal transduction pathway of the mammalian host. Three novel tRNA genes present in the phage genome may serve to increase the availability of rare tRNA species associated with efficient expression of pathogenicity determinants: both the Shiga toxin and serine/threonine kinase genes contain rare isoleucine and arginine codons. 933W also has homology to lom, encoding a member of a family of outer membrane proteins associated with virulence by conferring the ability to survive in macrophages, and bor, implicated in serum resistance.  (+info)

Induction of prophages of enterohemorrhagic Escherichia coli O157:H7 with norfloxacin. (6/3698)

Norfloxacin (NFLX) caused induction of prophages VT1 and VT2 of enterohemorrhagic Escherichia coli O157 at subinhibitory concentrations. In time course experiments, we observed the following sequential events: upon induction, the phage genomes underwent multiplication; the amount of stx genes increased; and subsequently, large quantities of toxins VT1 and VT2 were produced. Further studies showed that the molecular mechanism of prophage induction is closely related to the RecA system since the prophage VT2 was not induced with NFLX in a recA mutant strain.  (+info)

A complex control circuit. Regulation of immunity in temperate bacteriophages. (7/3698)

Temperate bacteriophages can display in a stable way two essentially different behaviours. In the immune state, a gene (cI) produces a repressor which prevents expression of all the other viral genes; in the non-immune state the typically viral functions are expressed. The choice between the two pathways and the establishment of one of them have much in common with cell determination and differentiation. This choice depends on a complex control system, in fact one of the most intricate nets of regulation known in some detail. Our paper provides a formal description and partial analysis of this regulatory net. It is shown that even for relatively simple known models, this kind of analysis uncovers predictions which had previously remained hidden. Some of these predictions were checked experimentally. The experimental part chiefly deals with the efficiency of lysogenization by thermoinducible lambda phage carrying mutations in one or more of the regulatory genes, N, cro and cII. Although N- mutations are widely known for preventing efficient integration, and both N- and cII mutations for preventing efficient establishment of immunity, it is shown that, as predicted by a simple model, both N- and cII- phage efficiently lysogenize at low temperature if they are in addition cro-. In contrast with lambda N- cro+, lambda N- cro- is not propagated as a plasmid at low temperature, precisely because it establishes immunity too efficiently. Genetic control circuits are described in terms of sets of logic equations, which relate the state of expression of genes or of chemical reactions (functions) to input (genetic and environmental) variables and to the presence of gene and reaction products (internal, or memorization varibles). From the set of equations, one derives a matrix which shows the stable stationary states (if any) of the system, and from which one can derive the pathways (temporal sequences of states) consistent with the model. This kind of analysis is complementary to the more widely used analysis based on differential equations; it allows one to analyze in less detail more complex systems. The language might be used as well, mutatis mutandis, in fields very different from genetics. The last part of the discussion deals with the role of positive feedback loops in our specific problem (establishment and maintenance of immunity in temperate bacteriophages) and in developmental genetics in general. As a generalization of an old idea, it is suggested that cell determination (for a given character) depends on a set of genes whose interaction constitutes a positive feedback loop. Such a system has two stable stationary states: which one is chosen will usually depend on additional controls grafted on the loop.  (+info)

Filamentous phage replication initiator protein gpII forms a covalent complex with the 5' end of the nick it introduced. (8/3698)

Rolling circle type DNA replication is initiated by introduction of a nick in the leading strand of the origin by the initiator protein, which in most cases binds covalently to the 5' end of the nick. In filamentous phage, however, such a covalent complex has not been detected. Using a suitable substrate and short reaction time, we show that filamentous phage initiator gpII forms a covalent complex with nicked DNA, which rapidly dissociates unless gpII is inactivated. A peptide-DNA complex was isolated from trypsin digest of the complex by ion-exchange column chromatography and gel filtration, and its peptide sequence was determined. The result indicated that gpII was linked to DNA by the tyrosine residue at position 197 from the N-terminus. The mutant protein in which this tyrosine was replaced by phenylalanine did not show any detectable activity to complement gene II amber mutant phage in vivo. In vitro, the mutant protein recognized the origin and bent DNA as well as the wild-type does, but failed to introduce a nick and to relax the superhelicity of cognate DNA.  (+info)

Escherichia coli bacteriophage lambda ATCC ® 77359™ Designation: pLDR10 TypeStrain=False Application: contains sequence attachment site integrating vector
Escherichia coli bacteriophage T4 ATCC ® 11303-B4™ Designation: T4 TypeStrain=False Application: Testing of aerosol containment on cell sorters
In the original article it was suggested that bacterial debris controls exhibited no cytokine response when incubated with PBMC. However, a subsequent data audit and additional statistical analysis has revealed that a number of the bacterial debris controls exhibited a positive cytokine response whereas others not, resulting in an inflated mean particularly for the TNF-α response (Supplementary table 1). These means were not significantly different to the response generated by the purified phages. This does not impact on the data presented or the statistical analysis that has been performed as part of Figure 2 (analysis was compared to commercial LPS or medium only) or any of the other analysis performed as part of the manuscript. However, it does mean that the bacterial debris controls are not suitable for showing the efficacy of the phage purification process and as such a component of the cytokine response generated may be due to remaining bacterial debris as suggested by Dufour et al. (2016)
Isolation, Molecular Characterization and Insight into the Genome Sequence of E. coli Bacteriophage ADB-2 from Poultry Fecal Sample Abstract.
SUGGESTED CITATION. German Council for Scientific Information Infrastructures (RfII): Statement on the proposals for a European Open Science Cloud (EOSC), Göttingen, 2018, 2 p.. Direct links to the document. ...
In our country Diarrheal epidemics occur seasonally. Two peaks of outbreaks agreeably coincide with dry season and monsoon rain. Several factors control the outbreaks to occur and collapse. Bacteriophages are one of them which have been reported to trigger the collapse of the outbreaks. The concentration of the Vibrio cholerae specific bacteriophages is inversely correlated with the concentration of Vibrio cholerae in the environment. Therefore bacteriophages probably play an essential role in controlling the epidemics to occur or collapse. It is still not clear what factors trigger the onset of Diarrheal outbreaks. This study was design to see the effect of E. coli bacteriophages on the epidemics of Diarrheal disease. Routine isolation, estimation and molecular characterization reveal the prevalence E. coli phage. We have tried to characterize the isolated phages by analyzing the DNA using the technique called restriction fragments length polymorphism (RFLP ...
A suspension of MS2, an Escherichia coli bacteriophage virus, was used to artificially contaminate the hands of participants prior to using three different handdrying devices: jet air dryer, warm air dryer, paper towel dispenser. Virus was detected by plaque formation on agar plates layered with the host bacterium. Vertical dispersal of virus was assessed at a fixed distance (0.4 m) and over a range of different heights (0.0 - 1.8 m) from the floor. Horizontal dispersal was assessed at different distances of up to three metres from the hand-drying devices ...
The major coat protein of bacteriophage M13 is synthesized as a precursor, the procoat, with a typical leader (signal) sequence of 23 residues at its NH2-terminus. A fusion protein that contains the NH2-terminal 141 residues of cytoplasmic ribulokinase and all but the first ten residues of M13 procoat was made. The fusion protein inserts into the plasma membrane of Escherichia coli and is processed by leader peptidase to give rise to a leader peptide of 155 residues and the mature coat protein of 50 residues. The NH2-terminus of the leader peptide remains in the cytoplasm and is protected from protease added to the medium outside of the cell. This indicates that M13 procoat inserts into the membrane as a loop structure and that the NH2-terminus of a leader peptide remains within the cytoplasm during membrane insertion. ...
This HMM represents a family of phage and plasmid replication proteins. In bacteriophage IKe and related phage, the full-length protein is designated gene II protein. A much shorter protein of unknown function, translated from a conserved in-frame alternative initiator, is designated gene X protein. Members of this family also include plasmid replication proteins. This model is built as a fragment model to better detect translations from alternate intiators and other fragments relative to full length gene II protein ...
Growth characteristics of coliphage viruses indicate that they are adapted to live with their Eschericia coli hosts in the intestinal tract. However, coliphage experimentally introduced by ingestion persist only transiently if at all in the gut of humans and other animals. This study attempted to identify the barriers to long term establishment of exogenous coliphage in the gastrointestinal (GI) tracts of laboratory mice. Intestinal contents were screened for the presence of coliphage and host bacteria, and strains of E. coli bacteria from different segments of the GI tract were tested for susceptibility to six common laboratory coliphages. Contrary to expectations, coliphage were not evident in the GI tracts of laboratory mice, although they were occasionally detected in feces. Commensal flora showed extreme variability within groups of mice despite identical handling and diet. Less than 20% of 48 mice tested carried E. coli in their gut, and of 22 commensal E. coli strains isolated and tested, 59%
Despite phages ubiquitous presence and great importance in shaping microbial communities, little is known about the diversity of specific phages in different ecological niches. Here, we isolated, sequenced, and characterized 38 Escherichia coli-infecting phages (coliphages) from poultry faeces to gain a better understanding of the coliphage diversity in the poultry intestine.
Das zweite „große Positionspapier des RfII befasst sich mit der Qualitätssicherung und -steigerung bei der Dokumentation von Forschungsdaten. Im Hauptteil werden verschiedene Qualitätskonzepte aufbereitet sowie die zahlreichen Herausforderungen bei der Verzahnung von Forschungsprozess und Datenlebenszyklus benannt. Empfehlungen richten sich an Wissenschaft und Politik gleichermaßen. Sie zielen u.a. auf eine zielgerichtete Verankerung des Themenfelds in den fachlichen Methodiken, eine Reputationssteigerung für wissenschaftliches Engagement zur Erhöhung der Forschungsdatenqualität und entsprechende Förderungen durch Zuwendungs- und Drittmittelgeber.. ...
Phix Doctor UV Curing Light allows you to fix surfboard ding repairs indoors on cloudy and rainy days when you use UV/Sun/Solar Cure Resin
That communication can occur between virus-infected cells has been appreciated for nearly as long as has virus molecular biology. The original virus communication process specifically was that seen with T-even bacteriophages-phages T2, T4, and T6-resulting in what was labeled as a lysis inhibition. Another proposed virus communication phenomenon, also seen with T-even phages, can be described as a phage-adsorption-induced synchronized lysis-inhibition collapse. Both are mediated by virions that were released from earlier-lysing, phage-infected bacteria. Each may represent ecological responses, in terms of phage lysis timing, to high local densities of phage-infected bacteria, but for lysis inhibition also to locally reduced densities of phage-uninfected bacteria. With lysis inhibition, the outcome is a temporary avoidance of lysis, i.e., a lysis delay, resulting in increased numbers of virions (greater burst size). Synchronized lysis-inhibition collapse, by contrast, is an accelerated lysis which is
This thesis analyzes the interaction of two DNA-binding proteins with the plus strand replication origin of bacteriophage f1. The origin has a bipartite structure consisting of a required core origin region and an adjacent A +T- rich enhancer sequence that potentiates replication approximately 100-fold. The core origin binds the initiator protein, and the enhancer contains three binding sites for the E. coli integration host factor (IHF). Both activator proteins bend the DNA sequence to which they bind, implying that together they wrap the origin DNA into a higher order structure that is active in initiation. The replication initiator protein of bacteriophage f1 (gene II protein) is a multifunctional protein that participates in DNA replication at a number of levels. The gene II protein binds to the core origin in a novel two-step fashion. The first binding step involves interaction of two gene II protein molecules with an inverted repeat (β- γ) at the center of the core origin to form a binding
TY - JOUR. T1 - Size exclusion-based purification and PCR-based quantitation of MS2 bacteriophage particles for environmental applications. AU - Farkas, Kata. AU - Varsani, Arvind. AU - Marjoshi, Delphine. AU - Easingwood, Richard. AU - McGill, Erin. AU - Pang, Liping. N1 - Funding Information: This work was funded by the Royal Society of New Zealand (Marsden Grant ESR-1001 ). D. Marjoshi was supported by a summer scholarship provided by the Royal Society of New Zealand and the University of Canterbury, New Zealand. The authors thank the following people for their support and assistance: R. Fredericks (University of Canterbury, New Zealand), W. Williamson and M. Mackenzie (Institute of Environmental Science & Research Ltd, New Zealand). PY - 2015/3/1. Y1 - 2015/3/1. N2 - MS2 bacteriophage is the most commonly used surrogate for pathogenic viruses in laboratory and field studies. In order to determine the number of infectious viral particles in samples, the use of accurate quantitation methods is ...
Biosynthesis of streptomyces griseus phage deoxyribonucleic acid by Judith Ann Shearer; 1 edition; First published in 1968; Subjects: DNA, Bacteriophages
Podana liczba cytowań wynika z analizy informacji dostępnych w Internecie i jest zbliżona do wartości obliczanej przy pomocy systemu Publish or Perish. ...
Those poor male bacteria! They have to contend with invading filamentous phage - something that Rogaine just cant cure! Well be talking more about male and female bacteria in a later lecture (sex in bacteria isnt quite the same concept as in eukaryotes).. What is significant here is that the virion of the filamentous phage (i.e. the viral particle) carries a single-strand of DNA - not a double helix. In the cell, this single-stranded genome (2.) is used as a template to synthesize a double-stranded replicative form (RF), which is essentially a plasmid (3.). The replicative form is used as a template to generate new single-stranded genomes (4.) that are packaged into virions (5.) to generate new phage. The cell doesnt die - it just grows more slowly and continues to secrete phage indefinitely.. The practical side of this story - if you use a cloning vector that is based on a filamentous bacteriophage (such as M13mp18 which is an engineered version of the phage M13) or merely contains an ...
Figure 8. Electrophoresis of Cytomedins in 1 % agarose gel after phenol-detergent extraction. Track 1 - molecular weight marker - bacteriophage lambda DNA treated with restriction endonuclease Hind III;. Track 2 - native DNA isolated from human blood lymphocytes (positive control);. Track 3 - Cortexin;. Track 4 - Retinalamin. The absence of luminescent material during ethidium bromide staining evidenced the lack of nucleic acids in bioregulators Cortexin and Retinalamin.. Cytamins were examined by the same method. The obtained results are exhibited in Figures 9 and 10. In Figure 9, the last tracks on the right and on the left display bacteriophage lambda DNA (molecular weight marker) treated with endonuclease Hind III. The second track on the left contains native DNA isolated by phenol-detergent method from human stomach mucous membrane (surgical material). Electrophoregram shows native human DNA as a discrete stripe above the bacteriophage lambda DNA fragment with the molecular weight of 23,000 ...
Escherichia coli ATCC ® 700078™ Designation: C Na1(r) TypeStrain=False Application: Coliphages in water Detection of bacteriophages Host strain for detection of somatic coliphages Water testing
Accepted name: dCTP diphosphatase. Reaction: dCTP + H2O = dCMP + diphosphate. Other name(s): deoxycytidine-triphosphatase; dCTPase; dCTP pyrophosphatase; deoxycytidine triphosphatase; deoxy-CTPase; dCTPase. Systematic name: dCTP nucleotidohydrolase. Comments: Also hydrolyses dCDP to dCMP and phosphate.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9024-87-7. References: 1. Zimmerman, S.B. and Kornberg, A. Deoxycytidine di- and triphosphate cleavage by an enzyme formed in bacteriophage-infected Escherichia coli. J. Biol. Chem. 236 (1961) 1480-1486.. ...
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1GVP: Analyses of the stability and function of three surface mutants (R82C, K69H, and L32R) of the gene V protein from Ff phage by X-ray crystallography.
Holin of 77 aas and 1 central TMS from E. coli phage ECBP5, Orf46. This protein is nearly identical to the pin-holin characterized for E. coli phage KBNP1315 (Lee et al. 2015). It infects a pathogenic avian E. coli strain (Lee et al. 2015 ...
Extensive nucleotide sequence data is new known for six small isometric phages, ([o with diagonal slash]Xl 74, G4, St-1, Sl3, [lowercase alpha]3 and [phi]K).Comparison of these sequences allows us to observe the role of ...
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1.Definición de Célula.  Es la unidad anatómico y funcional de todo ser vivo.  Tiene función de autoconservación y autorreproducción.  Es por esto, por lo q…
The bacteriophage MS2 is an icosahedral, positive-sense single-stranded RNA virus that infects the bacterium Escherichia coli and other members of the Enterobacteriaceae. MS2 is a member of a family of closely related bacterial viruses that includes bacteriophage f2, bacteriophage Qβ, R17, and GA. In 1961, MS2 was isolated by Alvin John Clark and recognized as an RNA-containing phage very similar to bacteriophage f2. In 1976, the MS2 genome was the first genome to be completely sequenced. This was accomplished by Walter Fiers and his team, building upon their earlier milestone in 1972 of the first gene to be completely sequenced, the MS2 coat protein. These sequences were determined at the RNA level, whereas the next landmark achievement, the sequence of the bacteriophage ΦX174 genome in 1977, was determined using DNA. The first effort at a statistical analysis of the MS2 genome was a search for patterns in the nucleotide sequence. Several non-coding sequences were identified, however at the ...
TY - JOUR. T1 - Replication of bacteriophage lambda DNA. AU - Enquist, L. W.. AU - Skalka, A. M.. N1 - Copyright: Copyright 2014 Elsevier B.V., All rights reserved.. PY - 1978/10. Y1 - 1978/10. N2 - When coliphage Lambda replicates its DNA, it commandeers portions of the host replication system for its own use. How phage and host functions interact and are regulated present interesting problems in the DNA replication program of phage Lambda.. AB - When coliphage Lambda replicates its DNA, it commandeers portions of the host replication system for its own use. How phage and host functions interact and are regulated present interesting problems in the DNA replication program of phage Lambda.. UR - http://www.scopus.com/inward/record.url?scp=0018071515&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0018071515&partnerID=8YFLogxK. U2 - 10.1016/S0968-0004(78)96033-4. DO - 10.1016/S0968-0004(78)96033-4. M3 - Review article. AN - SCOPUS:0018071515. VL - 3. SP - 279. EP - ...
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I am based in the laboratory of Assoc Prof Keith Shearwin (Biochemistry, Molecular and Biomedical Science) https://researchers.adelaide.edu.au/profile/keith.shearwin.. Research in the Shearwin lab integrates biochemistry, genetics and mathematical modelling to characterise fundamental mechanisms of gene control and how these mechanism are combined to create gene regulatory circuits with complex functions. Our primary experimental systems are two E. coli bacteriophages, lambda and 186. These temperate phages can replicate their genomes using alternative developmental pathways, lysis and lysogeny, and are some of the simplest organisms to make developmental decisions. Despite their relative simplicity, the phage systems combine a wide range of gene control mechanisms in complex ways and have many lessons to teach us.. The phage systems have been the springboard for my particular interests in DNA looping, molecular traffic on DNA and epigenetics.. DNA loops are created when proteins bound to ...
The DNA in its circular form contains 48,502 base-pairs...Bacteriophage lambda DNA in its circular form contains 48,502 base-pairs and codes for about 60 proteins. According to abstract phage Lambda has 46 clearly defined ORFs and another ~20 putative ORFs, totaling ~66ORFs (~60 proteins according to p.730 2nd paragraph). The number of bases is for the double stranded circular chromosome. 48,514 bp according to Lewin, Genes VIII, 2004, p.335 fig.12.11 ...
Bacteria and their viruses (phages) are abundant across diverse ecosystems and their interactions influence global biogeochemical cycles and incidence of disease. Problematically, both classical and metagenomic methods insufficiently assess the host specificity of phages and phage-host infection dynamics in nature. Here we review emerging methods to study phage-host interaction and infection dynamics with a focus on those that offer resolution at the single-cell level. These methods leverage ever-increasing sequence data to identify virus signals from single-cell amplified genome (SAG) datasets or to produce primers/probes to target particular phage- bacteria pairs (digital PCR and phageFISH), even in complex communities. All three methods enable study of phage infection of uncultured bacteria from environmental samples, while the latter also discriminates between phage-host interaction outcomes (e.g., lytic, chronic, lysogenic) in model systems. Together these techniques enable quantitative,
When copper salts are mixed with substances containing thiol groups, complexes are formed whose composition is determined by the concentration of the two components. After mixing copper and thiol compounds in certain concentrations the mixtures became strongly virucidal for T3, T5 and φX 174 phages, while T2 and T4 were affected only at higher concentrations of copper. Unless otherwise stated, coliphage T5 was used as test organism in these experiments. Together with a final concentration of 5 × 10-5 m-CuCl2 the thiol compounds cysteine and dithio-pentaerythrite at certain concentrations had a strong virucidal activity, while no such activity was demonstrated for the corresponding disulphide compounds (Fig. 1). Dithio-pentaerythrite but not cysteine was active with a CuCl2 concentration of 5 × 10-6 m. At this copper concentration thioglycollic acid, 2,3-dimercaptopropanol and dihydrothioctic acid were also virucidal, while glutathione was active only with 5×10-5 m-CuCl2, and the disulphide compound
Usually bacteriophages lyse their hosts following infection, however a few so-called temperate phage undergo lysogeny. In lysogeny, the bacteriophage integrates its genome into that of its host. The phage, then, is replicated each time the bacterial cell divides. In the lysogenic state, the bacteriophage can have considerable influence over host physiology ...
Heineman et al. found that T7 phage could evolve the ability to discriminate between several host strains. T7wild-type was independently adapted in two mixes of Escherichia coli strains: C with either B or K12. In both adaptations, C was the permissive host, while the other (B or K12, depending on the adaptation) aborted T7 infections due to deletion of a host gene needed for viral replication. Both adapted phages evolved to largely avoid the nonpermissive host but maintained a high adsorption rate to C. Notably the phage evolved the ability to discriminate via single amino acid substitutions in the tail fiber gene (used to bind host receptors ...
- SS2378646 A bacteriophage, comprising a proteic envelope (called capsid), which contains its nucleic acid (DNA or RNA), and a tail. The tail includes a collar (covered with contractile proteins for the most elaborated bacteriophages, such as the T2 and T4 phages) and ending with tail fibers enabling it to attach to the bacteria it infects.
Regulation by a cascade in phages, Regulation of Gene Expression Cricuit of Lytic Cycle and Lysogeny in Bacteriophages, Genetics
A stylized image of a bacteriophage. The capsid, tail, tail fibers, base plague and contractile shield are shown. - Stock Image F002/0293
First, related to the question at the beginning of the thread, I do not think you have to take this into account: Hes talking about bacteriophage, You just a sensitive strain of bacteria, the one used for propagating the phage would be good ...
Bacteriophages (phages) are probably the most abundant entities in nature, often exceeding bacterial densities by an order of magnitude. As viral predators
Gentaur molecular products has all kinds of products like :search , bio-gentaur \ MS2 Bacteriophage \ GEN0810066 for more molecular products just contact us
నెల్లూరు: మనుబోలు మండలం బద్వేలు క్రాస్‌రోడ్డు దగ్గర కారు బోల్తా, ముగ్గురికి గాయాలు,కర్నూలు: 16 వ రోజు జగన్ ప్రజా సంకల్ప యాత్ర,రంగారెడ్డి: మైలార్‌దేవ్‌పల్లిలో కింగ్స్‌ కాలనీలో ముస్తఫా అనే వ్యక్తిపై దుండగుల కాల్పులు,కడప: జగన్ సీఎం అయితే తన ఆస్తులు పెరుగుతాయి..చంద్రబాబు సీఎంగా ఉంటే ప్రజల ఆస్తులు పెరుగుతాయి: మంత్రి సోమిరెడ్డి,సిరిసిల్ల: అన్ని గ్రామాల్లో కేసీఆర్ గ్రామీణ ప్రగతి ...
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The usefulness of a recombinant phage library depends on the ability to screen a large number of phage and identify the clone that carries the DNA sequence of interest
New Beachcomber 2 by Lula Bijoux & Company: This New Beachcomber 2 Fine Art Print and related works can be found at FulcrumGallery.com.
1.Experiments by the following scientists provided critical information concerning DNA. Fully describe 2 of these 3 classical experiments and indicate how each provided evidence for the chemical nature of the gene.a. Hershey and Chase- bacteriophage re...
Для выдвижного усилия поршня шприца в инъекционных насосах VIT-FIT и насосах VIT-FIT HP высокого давления установлен качественный швейцарский двигатель. Его новая технология обеспечивает высокий крутящий момент и на порядок более долгий срок службы. Для того чтобы перевести вращательное движение в линейное перемещение применяется шариковинтовая пара, выдерживающая большие механические нагрузки. Тем не менее, эти дорогостоящие компоненты играют решающую роль в обеспечении жесткости хода толкателя. Это важно для неизменной непульсирующей линейной ...
2021년, 올해는 블록체인의 위기를 해결하고 본격적인 대중화를 위한 기회가 될 것으로 예상합니다. 람다256은 그 간 많은 고객과 정부 기관을 만났으며 다양한 현장 문제 해결에 블록체인 기술을 적용하기 위해 많은 노력을 기울였습니다. 이러한 노력과 경험을 통해 2021년 눈여겨볼 5가지 블록체인 분야를 전망해 봅니다.
A coliphage is a type of bacteriophage that infects coliform bacteria such as Escherichia coli. Coliphage originate almost ... Coliphage levels reflect the persistence of pathogenic viruses in the environment and have been proposed as an indicator of ... Coliphages at the US National Library of Medicine Medical Subject Headings (MeSH) A science project targeted towards students ... Nappier SP, Hong T, Ichida A, Goldstone A, Eftim SE (April 2019). "Occurrence of coliphage in raw wastewater and in ambient ...
Cross resistance amongst coliphages (PhD thesis). University of Adelaide. Retrieved 2021-07-25. Hancock, R.E.W. (2001). " ...
Pratt D, Tzagoloff H, Beaudoin J (September 1969). "Conditional lethal mutants of the small filamentous coliphage M13. II. Two ... Pratt D, Tzagoloff H, Erdahl WS (November 1966). "Conditional lethal mutants of the small filamentous coliphage M13. I. ... "Ff coliphages: structural and functional relationships". Microbiological Reviews. 50 (4): 401-27. doi:10.1128/MR.50.4.401- ...
Fletcher RD (1965). "Activity and morphology of Vibrio coli phage". American Journal of Veterinary Research. 26 (111): 361-4. ...
Suan, Sim Tiow; Chuen, Ho Yueh; Sivaborvorn, Komol (September 9, 1988). "Southeast Asian experiences with the coliphage test". ...
Alterations of receptor specificities of coliphages of the T2 family. J. Mol. Biol. 240:105-110. The older phage literature, e. ...
Chow, S; Daub, E; Murialdo, H (1987). "The Overproduction of DNA Terminase of Coliphage Lambda". Gene. 60 (2-3): 277-289. doi: ...
Walker, J T; D H Walker (March 1983). "Coliphage P1 morphogenesis: analysis of mutants by electron microscopy". Journal of ...
Barondess JJ, Beckwith J (August 1990). "A bacterial virulence determinant encoded by lysogenic coliphage lambda". Nature. 346 ...
Pratt D, Tzagoloff H, Beaudoin J (September 1969). "Conditional lethal mutants of the small filamentous coliphage M13. II. Two ... Pratt D, Tzagoloff H, Erdahl WS (November 1966). "Conditional lethal mutants of the small filamentous coliphage M13. I. ...
Pratt, D. (1969). "Conditional lethal mutants of the small filamentous coliphage M13: II. Two genes for coat proteins". ...
"Horizontal gene transfer and the evolution of microvirid coliphage genomes". Journal of Bacteriology. 188 (3): 1134-42. doi: ...
Stojković EA, Rothman-Denes LB (2007). "Coliphage N4 N-acetylmuramidase defines a new family of murein hydrolases". J Mol Biol ...
Horizontal Gene Transfer and the Evolution of Microvirid Coliphage Genomes. Journal of Bacteriology, 118(3) p1134-1142 ...
Additionally, Gp9 of E. coli phage phiE49 is similar in sequence. These proteins are short, 55 to 71 amino acyl residues (aas) ...
Chapman-McQuiston, E. (2007). "The Effect of Noisy Protein Expression on E. coli/Phage Dynamics". {{cite journal}}: Cite ...
Kozak, M; Nathans, D (14 September 1972). "Differential inhibition of coliphage MS2 protein synthesis by ribosome-directed ...
Some types of coliphages (a type of bacteriophage) are inactive in an of air-water-solid interface. This is due to the ... In a experiments that used E.coli phages, Qβ, fr, T4, and MS2 confirmed that viruses survive on a solid surface longer compared ...
Tomlinson S, Taylor PW (August 1985). "Neuraminidase associated with coliphage E that specifically depolymerizes the ...
Birkeland, N.K. and B.H. Lindqvist, Coliphage P2 late control gene ogr: DNA sequence and product identification. Journal of ... 39(4): p. 839-860 Lindqvist, B.H., Vegetative DNA of temperate coliphage P2. Molecular and General Genetics, 1971. 110(2): p. ... Site-specific recombination links the evolution of P2-like coliphages and pathogenic enterobacteria. Molecular Biology and ...
Bruttin A, Brüssow H (July 2005). "Human volunteers receiving Escherichia coli phage T4 orally: a safety test of phage therapy ... February 2016). "Oral Phage Therapy of Acute Bacterial Diarrhea With Two Coliphage Preparations: A Randomized Trial in Children ... they investigated the oral administration of Escherichia coli phage T4 and they found no adverse effects of the treatment. ...
Barbirz S, Müller JJ, Uetrecht C, Clark AJ, Heinemann U, Seckler R (Jul 2008). "Crystal structure of Escherichia coli phage ...
These sediments release Copper and Chromium and have bactericidal properties that multiply coliphages reducing and ultimately ...
"Comparative genomics of the T4-Like Escherichia coli phage JS98: implications for the evolution of T4 phages". J. Bacteriol. ...
In virology, temperate refers to the ability of some bacteriophages (notably coliphage λ) to display a lysogenic life cycle. ...
... and coliphage HSA virus; each of enterocin AAR-71 class IIa, enterocin AAR-74 class IIa, and erwiniocin NA4 against coliphage ...
Nguyen, Huong Minh; Kang, Changwon (2014-02-01). "Lysis delay and burst shrinkage of coliphage T7 by deletion of terminator Tφ ...
In reference to E. coli phage lambda, the term is sometimes written as "χ site", using the Greek letter chi; for E. coli and ...
... coliphages). A system based on ultrafiltration system (unlike Reverse osmosis based units) is not able to filter out solutes ...
... destruction caused by the E. coli phage Rac and the P. aeruginosa prophage Pf4 causes detachment of cells from the ...
Šimková, A. & Červenka, J. (‎1981)‎. Coliphages as ecological indicators of enteroviruses in various water systems*. Bulletin ...
Genome sequence and characterisation of coliphage vB_Eco_SLUR29. Ibrahim Besler, Pavelas Sazinas, Christian Harrison, Lucy ... Genome sequence and characterisation of coliphage vB_Eco_SLUR29 Message Subject (Your Name) has forwarded a page to you from ... Despite this there is still much to be discovered about the diversity of coliphage genomes. Within this study we isolated a ... Bacteriophage that infect Escherichia coli are relatively easily isolated, with greater than 600 coliphage genomes sequenced to ...
Here, we investigated the resistance of MS2 coliphage to inactivation by chlorine dioxide (ClO2). ClO2 inactivates MS2 by ... Dive into the research topics of Genetic, Structural, and Phenotypic Properties of MS2 Coliphage with Resistance to ClO2 ... Genetic, Structural, and Phenotypic Properties of MS2 Coliphage with Resistance to ClO2 Disinfection. Environmental Science and ... Genetic, Structural, and Phenotypic Properties of MS2 Coliphage with Resistance to ClO2 Disinfection. In: Environmental Science ...
Coliphages infect coliform bacteria. Coliphages do not infect humans or cause illness. A positive test for coliphages indicates ... Coliphage: A virus that infects bacteria is called a phage. Phages infect specific species of bacteria. ... Fecal coliform indicators: Groups of microbes, such as E. coli, enterococci, and coliphage, used under the Groundwater Rule to ...
Coliphages / drug effects * Coliphages / genetics* * DNA, Viral / genetics* * DNA, Viral / immunology * Escherichia coli / ...
Coliphages as ecological indicators of enteroviruses in various water systems*  Šimková, A.; Červenka, J. (‎1981)‎ ...
Base-unpaired regions in supercoiled replicative form DNA of coliphage M13. S. Dasgupta, D. P. Allison, C. E. Snyder, S. Mitra ... Dive into the research topics of Base-unpaired regions in supercoiled replicative form DNA of coliphage M13. Together they ...
Somatic coliphages were detected in 98% of samples (geometric mean 24 +/- 4.1 PFU per 100 ml), and F+ coliphages were detected ... Hepatitis E virus and coliphages in waters proximal to swine concentrated animal feeding operations. ... and all of the F+ RNA coliphages belonged to genogroup I. Although the pervasiveness of swine CAFOs in this area prevented a ... and coliphages. HEV was detected in one sample. ... of the F+ coliphage isolates were RNA phage, ...
Molecular mechanism of heat shock-provoked disassembly of the coliphage lambda replication complex ... Molecular mechanism of heat shock-provoked disassembly of the coliphage lambda replication complex. Journal of Bacteriology, ...
The process of infection with coliphage T7. I. Characterization of T7 RNA by polyacrylamide gel electrophoretic analysis ( ... The process of infection with coliphage T7. I. Characterization of T7 RNA by polyacrylamide gel electrophoretic analysis. ...
Decay rates of Escherichia coli, Enterococcus spp., F-specific bacteriophage MS2, somatic coliphage and human adenovirus in ... Quantification and fate of plasmid-specific bacteriophages in wastewater: Beyond the F-coliphages ...
Coliphages as ecological indicators of enteroviruses in various water systems*  Šimková, A.; Červenka, J. (‎1981)‎ ...
The ICR might also require monthly monitoring for Clostridium perfringens and coliphage. + One organism per liter of water. & ...
Kozak, M. & Nathans, D. Fate of maturation protein during infection by coliphage MS2. Nat. New Biol. 234, 209-211 (1971) ...
Parameter could be either somatic coliphages or more likely male-specific coliphages (F+ RNA coliphages), for which a draft ... Standard for F+ RNA coliphages in water now being developed as ISO/DIS 10705-1; methods for somatic coliphages in draft stages ...
This procedure is so common as to be the basis for the standard method of screening for coliphage in water samples ( ... Enumeration of Somatic Coliphages. ISO 10705-2:2000(E). Geneva: International Organization for Standardization. ...
Virus: Escherichia coli phage (øX-174 ATCC 13706-B1) Influenza (H1lN1) 2009 Virus. KRCES-Env. Test Report no: 23_0140 Mould: ... Escherichia coli phage (øX-174 ATCC 13706-B1) In-Filter Deactivation was certified by Kitasato Research centre for ...
The filters performance could be worse for MS2 coliphages (virus) removal than E. coli (bacteria). KAF assessment at pilot ... coli and male-specific coliphages (MS2 virus) to achieve a target concentration of 104 CFU/100 mL and 105 PFU/100 mL, ...
Coliphages are used as indicators of virus-related fecal contamination and of the microbiological quality of waters. This ... Coliphages are used as indicators of virus-related fecal contamination and of the microbiological quality of waters. This ...
Method 1601 : Male-specific (F+) and somatic coliphage in water by two-step enrichment procedure : April 2000 draft.. 2000. ... Method 1602 : Male-specific (F+) and somatic coliphage in water by single agar layer (SAL) procedure : April 2000 draft.. 2000 ... Method 1601 : male-specific (F+) and somatic coliphage in water by two-step enrichment procedure.. 2001. ...
Complete genome sequence of Escherichia coli Phage vB_EcoS Sa179lw isolated from surface water in a produce-growing area in ... Complete genome sequence of Escherichia coli Phage vB_EcoS Sa179lw isolated from surface water in a produce-growing area in ... Complete genome sequence of Escherichia coli phage vB_EcoM Sa157lw, isolated from surface water collected in Salinas, ... Complete genome sequence of Escherichia coli phage vB_EcoM Sa157lw, isolated from surface water collected in Salinas, ...
... no F-specific coliphage viruses, pathogenic microorganisms or indicator microorganisms of fecal contamination are excluded from ... F-specific coliphage viruses, pathogenic microorganisms or indicator microorganisms of fecal contamination, have undergone a ... F-specific coliphage viruses, pathogenic microorganisms or indicator microorganisms of fecal contamination, the advisory to ... taken or stored that supplies the distribution system at least once a month to test for the presence of F-specific coliphage ...
Coliphages *Hydrocarbon utilizing bacteria. *Iron related bacteria. *Pseudomonas aeruginosa *Fecal and total coliforms ...
Synteny between predicted coliphages and their respective reference genomes were visualised using EasyFig [34]. Escherichia ... 2b). Interestingly, more than one genus of coliphage (e.g. Jilinvirus, Phapecoctavirus, or Gamaleyavirus) was often detected in ... Coverage values for four identified coliphages showing similarity to known bacteriophage genomes of Escherichia coli across the ... Complete genome sequence of the novel Escherichia coli phage phAPEC8. J Virol. 2012;86:13117-8. ...
report that coliphage G7C carries a TSP that deacetylates O-antigen but does not degrade it, whereas rough strains or strains ... Coliphages metabolism, Operator Regions, Genetic, Repressor Proteins chemistry, and Viral Regulatory and Accessory Proteins ...
At the beginning of WP1, while the production of most of the 28 phages (for both cocktails, i.e. 15 for the anti E. coli phages ... coli phage cocktail was stopped. Regulatory authorities were informed of that decision.. Finally, the authorisation to relaunch ...
Elimination of fecal coliforms and F-specific RNA coliphage from oysters (Crassostrea virginica) relaid in floating containers ...
  • Method 1601 : Male-specific (F+) and somatic coliphage in water by two-step enrichment procedure : April 2000 draft. (epa.gov)
  • Method 1602 : Male-specific (F+) and somatic coliphage in water by single agar layer (SAL) procedure : April 2000 draft. (epa.gov)
  • Bacteriophage that infect Escherichia coli are relatively easily isolated, with greater than 600 coliphage genomes sequenced to date. (biorxiv.org)
  • A positive test for coliphages indicates the water may be contaminated with feces or E. coli or viruses. (cdc.gov)
  • Coliphages have been recommended as more trustworthy indicators when it comes to finding viruses in water sources, learn more about coliphages and the detection of viruses. (labunlimited.com)
  • Samples were evaluated for levels of fecal contamina- tion by using fecal coliforms and somatic coliphages. (cdc.gov)
  • Here, we investigated the resistance of MS2 coliphage to inactivation by chlorine dioxide (ClO 2 ). (utmb.edu)
  • Coliphages infect coliform bacteria. (cdc.gov)
  • Coliphages do not infect humans or cause illness. (cdc.gov)
  • Somatic coliphages are those which infect cells. (cdc.gov)
  • The diversity of coliphages and indigenous coliform strains (ICSs) simultaneously within horse feces was investigated by culture-based and molecular methods. (hoot4owls.org)
  • Despite this there is still much to be discovered about the diversity of coliphage genomes. (biorxiv.org)
  • Somatic coliphages were enumerated according to can also transduce resistance genes from Salmonella enter- the standard method. (cdc.gov)
  • Hepatitis E virus and coliphages in waters proximal to swine concentrated animal feeding operations. (cdc.gov)
  • Surface water samples (n=154) were collected from public access waters in proximity to swine CAFO spray fields for six months and were tested for hepatitis E virus (HEV) and coliphages. (cdc.gov)
  • Why does WHO recommend testing for coliphages to ensure water quality? (bluephage.com)
  • Within this study we isolated a coliphage from cattle slurry collected from a farm in rural England. (biorxiv.org)
  • Bluephage provides a series of Easy Kits to analyze coliphages according to ISO and US-EPA methods, improving its performance as they are kits containing all the material requested and are ready to use, reducing the execution time considerably. (bluephage.com)
  • This study reports on the use of naturally occurring F-specific coliphages, as well as spiked MS-2 phage, to evaluate a land-based effluent treatment/reuse system and an effluent irrigation scheme. (qld.gov.au)
  • All three phage genomes included a homologue of the tum gene of coliphage 186, which encodes a LexA-repressed cI antirepressor. (elsevier.com)
  • Fast Phage is an EPA approved test for detecting coliphage in water that provides an early warning in 8 hours and is confirmed the next morning. (charm.com)
  • T4 coliphage is a phage that infect coliform bacteria especially E.coli . (biotechfront.com)
  • Samples were evaluated for levels of fecal contamina- tion by using fecal coliforms and somatic coliphages. (cdc.gov)
  • Somatic coliphages are those which infect cells. (cdc.gov)
  • Somatic coliphages were enumerated according to can also transduce resistance genes from Salmonella enter- the standard method. (cdc.gov)
  • Somatic coliphages were detected in 98% of samples (geometric mean 24 +/- 4.1 PFU per 100 ml), and F+ coliphages were detected in 85% of samples (geometric mean 6.8 +/- 5.0 PFU per 100 ml). (cdc.gov)
  • As traditional bacterial indicators are poor viral indicators, there is a need for alternative methods, such as the use of somatic coliphages, which have been included in water safety regulations in recent years. (helsinki.fi)
  • This host strain has been designed for its use in the detection and enumeration of somatic coliphages according to the ISO 10705-2 method. (ixole.es)
  • It can be used with the Enumeration of Somatic Coliphages Kit (Kit Cat. (ixole.es)
  • No. BP1601), the Enumeration of Somatic Coliphages in Drinking Water Kit (Cat. (ixole.es)
  • This positive control has been designed for its use in the detection and enumeration of somatic coliphages according to the ISO 10705-2,US-EPA 1601, 1602, 1642 and 1643 methods. (ixole.es)
  • It has been understood that the presence of coliphages (bacteriophages specific for E. coli) in water is an indirect measurement of fecal contamination. (sc.edu)
  • Charm's water quality diagnostics help protect and improve water quality by rapidly identifying fecal indicators and contaminants, such as coliphage, coliform, and E. coli, to identify "at risk" water supplies for viral and bacterial pathogens. (charm.com)
  • Coliphage is a virus particle to coliform bacteria and is considered a viral fecal indicator in ground water that complements bacterial fecal monitoring. (charm.com)
  • Hernández, N. and R. Rios, "Evaluation of Coliphage and Fecal Coliform Densities on the Principal Canal of the Irrigation System in Southwestern Puerto Rico, presented at the XXVIII AIDIS Congress, published in the Proceedings, Cancun, Mexico, October 2002. (uprrp.edu)
  • Coliphages infect coliform bacteria. (cdc.gov)
  • M. Martins, G. Castillo and B. J Dutka, "Evaluation of Drinking Water Treatment Plant Efficiency in Microorganism Removal by the Coliphage, Total Coliform and H2S Paper Strip Test," Water Science and Technology, Vol. 3, No. 35, 1997, pp. 403-407. (scirp.org)
  • A bacterial virulence determinant encoded by lysogenic coliphage lambda. (microbiologyresearch.org)
  • Lysogenic for coliphage lambda. (carolina.com)
  • Under the direction of Dr. Jill Stewart, Sharon studied the potential contribution of lysogenic phages in coliphage enumeration methods. (ladlab.org)
  • Seven wells tested positive for enterococci and Arcobacter (an emerging bacterial pathogen), and F + -specific coliphage was present in four wells. (nih.gov)
  • Polymerase chain reaction (PCR) analysis was conducted on all samples collected in order to characterize any possible coliphages identified via plaque assays. (sc.edu)
  • The rods are com-posed of two hollow cylindrical structures that are stri-king-ly similar to tail components - core and contracted sheath - of various phages, e.g., the T-even coliphages. (asmblog.org)
  • Comparison of coliforms and coliphages as tools for assessment of viral contamination in river water. (sigmaaldrich.com)
  • Coliphage may also be used as a viral process indicator in water recharge applications and waste water treatment before discharge. (charm.com)
  • EPA convened a group of twelve internationally acknowledged specialists on the state of the science of coliphage and their usefulness as a viral indicator for the protection of public well being in recreational waters. (thehazelbloom.com)
  • A positive test for coliphages indicates the water may be contaminated with feces or E. coli or viruses. (cdc.gov)
  • Among these discoveries was that T-even coliphages contract their tail upon absorption to their E. coli hosts. (asmblog.org)
  • This is the equivalent of the 2nd year prac I used to do, when we made students screen water obtained from the environment with E coli to see if they could amplify coliphages out of it. (rybicki.blog)
  • These findings confirm that there are indeed bacteriophages present in the Brookgreen Gardens aviary, and that pH, turbidity, and temperature did not appear to play a prominent role in the presence of the coliphages. (sc.edu)
  • Quantification of infectious MS2 coliphage was carried out using a double agar layer procedure. (umn.edu)
  • The ISO Easy Kit is designed to eliminate the tedious and time-consuming process of standardized ISO protocols for the detection and enumeration of somatic and F-specific coliphages. (ixole.es)
  • Our specially crafted ISO Easy Kits (BP1601 and BP1604) are designed to eiminate the tedious and time-consuming process of of the standardized ISO 10705-2 protocol for the detection and enumeration of both somatic and F-specific coliphages. (ixole.es)
  • Available for somatic and F-specific coliphage enumeration. (ixole.es)
  • This pilot study analyzes the relationship between the presence of coliphages in the water in the aviary at Brookgreen Gardens and how environmental factors such as temperature, pH, and turbidity affect the presence of these viruses over the time period of three months (June 2017 through August 2017). (sc.edu)
  • Comparison of our isolates to their closest relative, coliphage T5, revealed high overall synteny of the genomes and high conservation of the sequences of almost all structural proteins as well as of the other proteins with identified functions. (utmb.edu)
  • Rothman-Denes, L. B. / Purification and characterization of the coliphage N4-coded single-stranded DNA binding protein . (elsevier.com)
  • We have purified and characterized a single-stranded DNA binding protein (N4 SSB) induced after coliphage N4 infection. (elsevier.com)
  • The protein coats or ghosts of coliphage T2. (wikidata.org)
  • Hepatitis E virus and coliphages in waters proximal to swine concentrated animal feeding operations. (cdc.gov)
  • We also just received our other tests for Listeria and MS2 coliphage, which is a virus, and we are beyond excited at the results. (rovingblue.com)
  • Group III coliphages were only detected at the sampling site in the vicinity of the international boundary, indicating human faecal contamination. (elsevier.com)
  • All PCR tests conducted on samples were deemed negative for coliphage presence, indicating that these coliphages are especially unique or may be RNA-based. (sc.edu)
  • It contains all the consumables and biological material required to perform the analysis, including freeze-dried specific host-cells for the somatic coliphage group, which are ready for use after 120 min of incubation. (ixole.es)
  • When only biological materials are required, our calibrated, ready-to-use materials solution will help you perform coliphage analysis. (ixole.es)
  • As the New River traverses through the US region, groups I and IV coliphages were predominantly identified, but no human-specific genotypes were detected. (elsevier.com)
  • Plaque assays were conducted to look for the presence of the coliphages. (sc.edu)