Coliphages: Viruses whose host is Escherichia coli.Leviviridae: A family of bacteriophages that infects enterobacteria, CAULOBACTER, and PSEUDOMONAS. The genome consists of linear, positive-sense single-stranded RNA.RNA Phages: Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.Inoviridae: A family of rod-shaped or filamentous bacteriophages consisting of single-stranded DNA. There are two genera: INOVIRUS and PLECTROVIRUS.Water Pollution: Contamination of bodies of water (such as LAKES; RIVERS; SEAS; and GROUNDWATER.)Sewage: Refuse liquid or waste matter carried off by sewers.Water Microbiology: The presence of bacteria, viruses, and fungi in water. This term is not restricted to pathogenic organisms.Waste Disposal, Fluid: The discarding or destroying of liquid waste products or their transformation into something useful or innocuous.F Factor: A plasmid whose presence in the cell, either extrachromosomal or integrated into the BACTERIAL CHROMOSOME, determines the "sex" of the bacterium, host chromosome mobilization, transfer via conjugation (CONJUGATION, GENETIC) of genetic material, and the formation of SEX PILI.Pseudomonas Phages: Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.Bacteriophage P2: A species of temperate bacteriophage in the genus P2-like viruses, family MYOVIRIDAE, which infects E. coli. It consists of linear double-stranded DNA with 19-base sticky ends.Enterovirus: A genus of the family PICORNAVIRIDAE whose members preferentially inhabit the intestinal tract of a variety of hosts. The genus contains many species. Newly described members of human enteroviruses are assigned continuous numbers with the species designated "human enterovirus".Flocculation: The aggregation of suspended solids into larger clumps.GeeseFeces: Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.Bacteriophages: Viruses whose hosts are bacterial cells.Lysogeny: The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.Levivirus: A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.Enterobacteriaceae: A family of gram-negative, facultatively anaerobic, rod-shaped bacteria that do not form endospores. Its organisms are distributed worldwide with some being saprophytes and others being plant and animal parasites. Many species are of considerable economic importance due to their pathogenic effects on agriculture and livestock.Filtration: A process of separating particulate matter from a fluid, such as air or a liquid, by passing the fluid carrier through a medium that will not pass the particulates. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Viruses: Minute infectious agents whose genomes are composed of DNA or RNA, but not both. They are characterized by a lack of independent metabolism and the inability to replicate outside living host cells.RNA Viruses: Viruses whose genetic material is RNA.Environmental Monitoring: The monitoring of the level of toxins, chemical pollutants, microbial contaminants, or other harmful substances in the environment (soil, air, and water), workplace, or in the bodies of people and animals present in that environment.Chloroform: A commonly used laboratory solvent. It was previously used as an anesthetic, but was banned from use in the U.S. due to its suspected carcinogenicity.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.Viral Plaque Assay: Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.Air Microbiology: The presence of bacteria, viruses, and fungi in the air. This term is not restricted to pathogenic organisms.Fresh Water: Water containing no significant amounts of salts, such as water from RIVERS and LAKES.DNA Viruses: Viruses whose nucleic acid is DNA.Water Supply: Means or process of supplying water (as for a community) usually including reservoirs, tunnels, and pipelines and often the watershed from which the water is ultimately drawn. (Webster, 3d ed)Microbiological Techniques: Techniques used in microbiology.Rivers: Large natural streams of FRESH WATER formed by converging tributaries and which empty into a body of water (lake or ocean).Enterococcus: A genus of gram-positive, coccoid bacteria consisting of organisms causing variable hemolysis that are normal flora of the intestinal tract. Previously thought to be a member of the genus STREPTOCOCCUS, it is now recognized as a separate genus.Seawater: The salinated water of OCEANS AND SEAS that provides habitat for marine organisms.Virus Cultivation: Process of growing viruses in live animals, plants, or cultured cells.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Colony Count, Microbial: Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.Serotyping: Process of determining and distinguishing species of bacteria or viruses based on antigens they share.Ribonucleases: Enzymes that catalyze the hydrolysis of ester bonds within RNA. EC 3.1.-.Seasons: Divisions of the year according to some regularly recurrent phenomena usually astronomical or climatic. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)RNA, Viral: Ribonucleic acid that makes up the genetic material of viruses.Bacteria: One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Birds: Warm-blooded VERTEBRATES possessing FEATHERS and belonging to the class Aves.Viral Proteins: Proteins found in any species of virus.Reverse Transcriptase Polymerase Chain Reaction: A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.Nucleic Acid Conformation: The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.Hot Temperature: Presence of warmth or heat or a temperature notably higher than an accustomed norm.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

Marker effects on reversion of T4rII mutants. (1/3698)

The frequencies of 2-aminopurine- and 5-bromouracil-induced A:T leads to G:C transitions were compared at nonsense sites throughout the rII region of bacteriophage T4. These frequencies are influenced both by adjacent base pairs within the nonsense codons and by extracodonic factors. Following 2AP treatment, they are high in amber (UAG) and lower in opal (UGA) codons than in allelic ochre (UAA) codons. In general, 5BU-induced transitions are more frequent in both amber and opal codons than in the allelic ochre codons. 2AP- and 5BU-induced transition frequencies in the first and third positions of opal codons are correlated with those in the corresponding positions of the allelic ochre codons. Similarly, the frequencies of 2AP-induced transition in the first and second positions of amber codons and their ochre alleles are correlated. However, there is little correlation between the frequencies of 5BU-induced transitions in the first and second positions of allelic amber and ochre codons.  (+info)

Bacteriophage inactivation at the air-water-solid interface in dynamic batch systems. (2/3698)

Bacteriophages have been widely used as surrogates for human enteric viruses in many studies on virus transport and fate. In this investigation, the fates of three bacteriophages, MS2, R17, and phiX174, were studied in a series of dynamic batch experiments. Both MS2 and R17 readily underwent inactivation in batch experiments where solutions of each phage were percolated through tubes packed with varying ratios of glass and Teflon beads. MS2 and R17 inactivation was the result of exposure to destructive forces at the dynamic air-water-solid interface. phiX174, however, did not undergo inactivation in similar studies, suggesting that this phage does not accumulate at air-water interfaces or is not affected by interfacial forces in the same manner. Other batch experiments showed that MS2 and R17 were increasingly inactivated during mixing in polypropylene tubes as the ionic strength of the solution was raised (phiX174 was not affected). By the addition of Tween 80 to suspensions of MS2 and R17, phage inactivation was prevented. Our data suggest that viral inactivation in simple dynamic batch experiments is dependent upon (i) the presence of a dynamic air-water-solid interface (where the solid is a hydrophobic surface), (ii) the ionic strength of the solution, (iii) the concentration of surface active compounds in the solution, and (iv) the type of virus used.  (+info)

End group of naturally terminated and UV lesion terminated T7 in vitro RNA. (3/3698)

The 3' terminal nucleosides of RNA transcribed in vitro by E. coli RNA polymerase from T7 DNA and UV irradiated TN DNA were determined. The 3' terminal nucleoside of naturally terminated (t1 termination site) RNA cytidine. In the case of RNA terminated at UV lesions, it is cytidine in 0 per cent of the molecules and adenosine in the remaining 30 per cent. Cytidine trialcohols are labile in high concentrations of KOH and at high temperature and appear to convert to uridine.  (+info)

Evolutionary relationships among diverse bacteriophages and prophages: all the world's a phage. (4/3698)

We report DNA and predicted protein sequence similarities, implying homology, among genes of double-stranded DNA (dsDNA) bacteriophages and prophages spanning a broad phylogenetic range of host bacteria. The sequence matches reported here establish genetic connections, not always direct, among the lambdoid phages of Escherichia coli, phage phiC31 of Streptomyces, phages of Mycobacterium, a previously unrecognized cryptic prophage, phiflu, in the Haemophilus influenzae genome, and two small prophage-like elements, phiRv1 and phiRv2, in the genome of Mycobacterium tuberculosis. The results imply that these phage genes, and very possibly all of the dsDNA tailed phages, share common ancestry. We propose a model for the genetic structure and dynamics of the global phage population in which all dsDNA phage genomes are mosaics with access, by horizontal exchange, to a large common genetic pool but in which access to the gene pool is not uniform for all phage.  (+info)

Sequence of Shiga toxin 2 phage 933W from Escherichia coli O157:H7: Shiga toxin as a phage late-gene product. (5/3698)

Lysogenic bacteriophages are major vehicles for the transfer of genetic information between bacteria, including pathogenicity and/or virulence determinants. In the enteric pathogen Escherichia coli O157:H7, which causes hemorrhagic colitis and hemolytic-uremic syndrome, Shiga toxins 1 and 2 (Stx1 and Stx2) are phage encoded. The sequence and analysis of the Stx2 phage 933W is presented here. We find evidence that the toxin genes are part of a late-phage transcript, suggesting that toxin production may be coupled with, if not dependent upon, phage release during lytic growth. Another phage gene, stk, encodes a product resembling eukaryotic serine/threonine protein kinases. Based on its position in the sequence, Stk may be produced by the prophage in the lysogenic state, and, like the YpkA protein of Yersinia species, it may interfere with the signal transduction pathway of the mammalian host. Three novel tRNA genes present in the phage genome may serve to increase the availability of rare tRNA species associated with efficient expression of pathogenicity determinants: both the Shiga toxin and serine/threonine kinase genes contain rare isoleucine and arginine codons. 933W also has homology to lom, encoding a member of a family of outer membrane proteins associated with virulence by conferring the ability to survive in macrophages, and bor, implicated in serum resistance.  (+info)

Induction of prophages of enterohemorrhagic Escherichia coli O157:H7 with norfloxacin. (6/3698)

Norfloxacin (NFLX) caused induction of prophages VT1 and VT2 of enterohemorrhagic Escherichia coli O157 at subinhibitory concentrations. In time course experiments, we observed the following sequential events: upon induction, the phage genomes underwent multiplication; the amount of stx genes increased; and subsequently, large quantities of toxins VT1 and VT2 were produced. Further studies showed that the molecular mechanism of prophage induction is closely related to the RecA system since the prophage VT2 was not induced with NFLX in a recA mutant strain.  (+info)

A complex control circuit. Regulation of immunity in temperate bacteriophages. (7/3698)

Temperate bacteriophages can display in a stable way two essentially different behaviours. In the immune state, a gene (cI) produces a repressor which prevents expression of all the other viral genes; in the non-immune state the typically viral functions are expressed. The choice between the two pathways and the establishment of one of them have much in common with cell determination and differentiation. This choice depends on a complex control system, in fact one of the most intricate nets of regulation known in some detail. Our paper provides a formal description and partial analysis of this regulatory net. It is shown that even for relatively simple known models, this kind of analysis uncovers predictions which had previously remained hidden. Some of these predictions were checked experimentally. The experimental part chiefly deals with the efficiency of lysogenization by thermoinducible lambda phage carrying mutations in one or more of the regulatory genes, N, cro and cII. Although N- mutations are widely known for preventing efficient integration, and both N- and cII mutations for preventing efficient establishment of immunity, it is shown that, as predicted by a simple model, both N- and cII- phage efficiently lysogenize at low temperature if they are in addition cro-. In contrast with lambda N- cro+, lambda N- cro- is not propagated as a plasmid at low temperature, precisely because it establishes immunity too efficiently. Genetic control circuits are described in terms of sets of logic equations, which relate the state of expression of genes or of chemical reactions (functions) to input (genetic and environmental) variables and to the presence of gene and reaction products (internal, or memorization varibles). From the set of equations, one derives a matrix which shows the stable stationary states (if any) of the system, and from which one can derive the pathways (temporal sequences of states) consistent with the model. This kind of analysis is complementary to the more widely used analysis based on differential equations; it allows one to analyze in less detail more complex systems. The language might be used as well, mutatis mutandis, in fields very different from genetics. The last part of the discussion deals with the role of positive feedback loops in our specific problem (establishment and maintenance of immunity in temperate bacteriophages) and in developmental genetics in general. As a generalization of an old idea, it is suggested that cell determination (for a given character) depends on a set of genes whose interaction constitutes a positive feedback loop. Such a system has two stable stationary states: which one is chosen will usually depend on additional controls grafted on the loop.  (+info)

Filamentous phage replication initiator protein gpII forms a covalent complex with the 5' end of the nick it introduced. (8/3698)

Rolling circle type DNA replication is initiated by introduction of a nick in the leading strand of the origin by the initiator protein, which in most cases binds covalently to the 5' end of the nick. In filamentous phage, however, such a covalent complex has not been detected. Using a suitable substrate and short reaction time, we show that filamentous phage initiator gpII forms a covalent complex with nicked DNA, which rapidly dissociates unless gpII is inactivated. A peptide-DNA complex was isolated from trypsin digest of the complex by ion-exchange column chromatography and gel filtration, and its peptide sequence was determined. The result indicated that gpII was linked to DNA by the tyrosine residue at position 197 from the N-terminus. The mutant protein in which this tyrosine was replaced by phenylalanine did not show any detectable activity to complement gene II amber mutant phage in vivo. In vitro, the mutant protein recognized the origin and bent DNA as well as the wild-type does, but failed to introduce a nick and to relax the superhelicity of cognate DNA.  (+info)

*Coliphage

A coliphage is a type of bacteriophage that infects Escherichia coli. Examples include Bacteriophage lambda and Leviviridae. ... Coliphages at the US National Library of Medicine Medical Subject Headings (MeSH). ...

*Ff phages

Rasched, I; E Oberer (December 1986). "Ff coliphages: structural and functional relationships". Microbiological Reviews. 50 (4 ...

*Bacteriophage experimental evolution

Alterations of receptor specificities of coliphages of the T2 family. J. Mol. Biol. 240:105-110. The older phage literature, e. ...

*Helios Murialdo

doi:10.1016/0022-2836(84)90330-9. Chow, S; Daub, E; Murialdo, H (1987). "The Overproduction of DNA Terminase of Coliphage ...

*P1 phage

Walker, J T; D H Walker (March 1983). "Coliphage P1 morphogenesis: analysis of mutants by electron microscopy". Journal of ...

*Virulence-related outer membrane protein family

Barondess JJ, Beckwith J (August 1990). "A bacterial virulence determinant encoded by lysogenic coliphage lambda". Nature. 346 ...

*Glycoside hydrolase family 108

Stojković EA, Rothman-Denes LB (2007). "Coliphage N4 N-acetylmuramidase defines a new family of murein hydrolases". J Mol Biol ...

*Microviridae

Horizontal Gene Transfer and the Evolution of Microvirid Coliphage Genomes. Journal of Bacteriology, 118(3) p1134-1142 ...

*T1 holin family

Additionally, Gp9 of E. coli phage phiE49 is similar in sequence. These proteins are short, 55 to 71 amino acyl residues (aas) ...

*Developmental noise

Chapman-McQuiston, E. (2007). "The Effect of Noisy Protein Expression on E. coli/Phage Dynamics". Blake, W. J.; et al. (2006 ...

*Marilyn Kozak

Kozak, M; Nathans, D (14 September 1972). "Differential inhibition of coliphage MS2 protein synthesis by ribosome-directed ...

*Endo-alpha-sialidase

Tombinson, S.; Taylor, P.W. (1985). "Neuraminidase associated with coliphage E that specifically depolymerizes the Escherichia ...

*Bacteriophage P2

Birkeland, N.K. and B.H. Lindqvist, Coliphage P2 late control gene ogr: DNA sequence and product identification. Journal of ... 39(4): p. 839-860 Lindqvist, B.H., Vegetative DNA of temperate coliphage P2. Molecular and General Genetics, 1971. 110(2): p. ... Site-specific recombination links the evolution of P2-like coliphages and pathogenic enterobacteria. Molecular Biology and ...

*Phage P22 Tailspike Protein

Barbirz S, Müller JJ, Uetrecht C, Clark AJ, Heinemann U, Seckler R (Jul 2008). "Crystal structure of Escherichia coli phage ...

*National Waterway 1

These sediments release Copper and Chromium and have bactericidal properties that multiply coliphages reducing and ultimately ...

*Enterobacteria phage T4

"Comparative genomics of the T4-Like Escherichia coli phage JS98: implications for the evolution of T4 phages". J. Bacteriol. ...

*T-even bacteriophages

T-even phages, also known as the E. coli phages, are a group of double-stranded bacteriophages from the family Myoviridae. ...

*Temperateness (virology)

In virology, temperate refers to the ability of some bacteriophages (notably coliphage λ) to display a lysogenic life cycle. ...

*T7 holin family

Nguyen, Huong Minh; Kang, Changwon (2014-02-01). "Lysis delay and burst shrinkage of coliphage T7 by deletion of terminator Tφ ...

*Portable Aqua Unit for Lifesaving

... coliphages). A system based on ultrafiltration system (unlike Reverse osmosis based units) is not able to filter out solutes ...

*Lambda phage

Enterobacteria phage λ (lambda phage, coliphage λ) is a bacterial virus, or bacteriophage, that infects the bacterial species ...

*Blue-white screen

Messing, J.; Gronenborn, B.; Müller-Hill, B.; Hans Hopschneider, P. (1977). "Filamentous coliphage M13 as a cloning vehicle: ...

*Autographivirinae

Since the 1990s, the term "T7 supergroup" has been coined for the expanding group of bacteriophages related to coliphage T7, as ...

*Foodborne illness

"Intra-laboratory validation of a concentration method adapted for the enumeration of infectious F-specific RNA coliphage, ...

*Lambda holin family

Neely, M. N.; Friedman, D. I. (1998-06-01). "Functional and genetic analysis of regulatory regions of coliphage H-19B: location ...
... Designation: pLDR10 TypeStrain=False Application: contains sequence attachment site integrating vector
In the original article it was suggested that bacterial debris controls exhibited no cytokine response when incubated with PBMC. However, a subsequent data audit and additional statistical analysis has revealed that a number of the bacterial debris controls exhibited a positive cytokine response whereas others not, resulting in an inflated mean particularly for the TNF-α response (Supplementary table 1). These means were not significantly different to the response generated by the purified phages. This does not impact on the data presented or the statistical analysis that has been performed as part of Figure 2 (analysis was compared to commercial LPS or medium only) or any of the other analysis performed as part of the manuscript. However, it does mean that the bacterial debris controls are not suitable for showing the efficacy of the phage purification process and as such a component of the cytokine response generated may be due to remaining bacterial debris as suggested by Dufour et al. (2016)
A suspension of MS2, an Escherichia coli bacteriophage virus, was used to artificially contaminate the hands of participants prior to using three different handdrying devices: jet air dryer, warm air dryer, paper towel dispenser. Virus was detected by plaque formation on agar plates layered with the host bacterium. Vertical dispersal of virus was assessed at a fixed distance (0.4 m) and over a range of different heights (0.0 - 1.8 m) from the floor. Horizontal dispersal was assessed at different distances of up to three metres from the hand-drying devices ...
The major coat protein of bacteriophage M13 is synthesized as a precursor, the procoat, with a typical leader (signal) sequence of 23 residues at its NH2-terminus. A fusion protein that contains the NH2-terminal 141 residues of cytoplasmic ribulokinase and all but the first ten residues of M13 procoat was made. The fusion protein inserts into the plasma membrane of Escherichia coli and is processed by leader peptidase to give rise to a leader peptide of 155 residues and the mature coat protein of 50 residues. The NH2-terminus of the leader peptide remains in the cytoplasm and is protected from protease added to the medium outside of the cell. This indicates that M13 procoat inserts into the membrane as a loop structure and that the NH2-terminus of a leader peptide remains within the cytoplasm during membrane insertion. ...
This HMM represents a family of phage and plasmid replication proteins. In bacteriophage IKe and related phage, the full-length protein is designated gene II protein. A much shorter protein of unknown function, translated from a conserved in-frame alternative initiator, is designated gene X protein. Members of this family also include plasmid replication proteins. This model is built as a fragment model to better detect translations from alternate intiators and other fragments relative to full length gene II protein ...
That communication can occur between virus-infected cells has been appreciated for nearly as long as has virus molecular biology. The original virus communication process specifically was that seen with T-even bacteriophages-phages T2, T4, and T6-resulting in what was labeled as a lysis inhibition. Another proposed virus communication phenomenon, also seen with T-even phages, can be described as a phage-adsorption-induced synchronized lysis-inhibition collapse. Both are mediated by virions that were released from earlier-lysing, phage-infected bacteria. Each may represent ecological responses, in terms of phage lysis timing, to high local densities of phage-infected bacteria, but for lysis inhibition also to locally reduced densities of phage-uninfected bacteria. With lysis inhibition, the outcome is a temporary avoidance of lysis, i.e., a lysis delay, resulting in increased numbers of virions (greater burst size). Synchronized lysis-inhibition collapse, by contrast, is an accelerated lysis which is
This thesis analyzes the interaction of two DNA-binding proteins with the plus strand replication origin of bacteriophage f1. The origin has a bipartite structure consisting of a required core origin region and an adjacent A +T- rich enhancer sequence that potentiates replication approximately 100-fold. The core origin binds the initiator protein, and the enhancer contains three binding sites for the E. coli integration host factor (IHF). Both activator proteins bend the DNA sequence to which they bind, implying that together they wrap the origin DNA into a higher order structure that is active in initiation. The replication initiator protein of bacteriophage f1 (gene II protein) is a multifunctional protein that participates in DNA replication at a number of levels. The gene II protein binds to the core origin in a novel two-step fashion. The first binding step involves interaction of two gene II protein molecules with an inverted repeat (β- γ) at the center of the core origin to form a binding
Biosynthesis of streptomyces griseus phage deoxyribonucleic acid by Judith Ann Shearer; 1 edition; First published in 1968; Subjects: DNA, Bacteriophages
Podana liczba cytowań wynika z analizy informacji dostępnych w Internecie i jest zbliżona do wartości obliczanej przy pomocy systemu Publish or Perish. ...
Those poor male bacteria! They have to contend with invading filamentous phage - something that Rogaine just cant cure! Well be talking more about male and female bacteria in a later lecture (sex in bacteria isnt quite the same concept as in eukaryotes).. What is significant here is that the virion of the filamentous phage (i.e. the viral particle) carries a single-strand of DNA - not a double helix. In the cell, this single-stranded genome (2.) is used as a template to synthesize a double-stranded replicative form (RF), which is essentially a plasmid (3.). The replicative form is used as a template to generate new single-stranded genomes (4.) that are packaged into virions (5.) to generate new phage. The cell doesnt die - it just grows more slowly and continues to secrete phage indefinitely.. The practical side of this story - if you use a cloning vector that is based on a filamentous bacteriophage (such as M13mp18 which is an engineered version of the phage M13) or merely contains an ...
Escherichia coli ATCC ® 700078™ Designation: C Na1(r) TypeStrain=False Application: Coliphages in water Detection of bacteriophages Host strain for detection of somatic coliphages Water testing
Accepted name: dCTP diphosphatase. Reaction: dCTP + H2O = dCMP + diphosphate. Other name(s): deoxycytidine-triphosphatase; dCTPase; dCTP pyrophosphatase; deoxycytidine triphosphatase; deoxy-CTPase; dCTPase. Systematic name: dCTP nucleotidohydrolase. Comments: Also hydrolyses dCDP to dCMP and phosphate.. Links to other databases: BRENDA, EXPASY, KEGG, Metacyc, PDB, CAS registry number: 9024-87-7. References: 1. Zimmerman, S.B. and Kornberg, A. Deoxycytidine di- and triphosphate cleavage by an enzyme formed in bacteriophage-infected Escherichia coli. J. Biol. Chem. 236 (1961) 1480-1486.. ...
Thank you for your interest in spreading the word about Biochemical Journal.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
1GVP: Analyses of the stability and function of three surface mutants (R82C, K69H, and L32R) of the gene V protein from Ff phage by X-ray crystallography.
Holin of 77 aas and 1 central TMS from E. coli phage ECBP5, Orf46. This protein is nearly identical to the pin-holin characterized for E. coli phage KBNP1315 (Lee et al. 2015). It infects a pathogenic avian E. coli strain (Lee et al. 2015 ...
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1.Definición de Célula.  Es la unidad anatómico y funcional de todo ser vivo.  Tiene función de autoconservación y autorreproducción.  Es por esto, por lo q…
The bacteriophage MS2 is an icosahedral, positive-sense single-stranded RNA virus that infects the bacterium Escherichia coli and other members of the Enterobacteriaceae. MS2 is a member of a family of closely related bacterial viruses that includes bacteriophage f2, bacteriophage Qβ, R17, and GA. In 1961, MS2 was isolated by Alvin John Clark and recognized as an RNA-containing phage very similar to bacteriophage f2. In 1976, the MS2 genome was the first genome to be completely sequenced. This was accomplished by Walter Fiers and his team, building upon their earlier milestone in 1972 of the first gene to be completely sequenced, the MS2 coat protein. These sequences were determined at the RNA level, whereas the next landmark achievement, the sequence of the bacteriophage ΦX174 genome in 1977, was determined using DNA. The first effort at a statistical analysis of the MS2 genome was a search for patterns in the nucleotide sequence. Several non-coding sequences were identified, however at the ...
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I am based in the laboratory of Assoc Prof Keith Shearwin (Biochemistry, Molecular and Biomedical Science) https://researchers.adelaide.edu.au/profile/keith.shearwin.. Research in the Shearwin lab integrates biochemistry, genetics and mathematical modelling to characterise fundamental mechanisms of gene control and how these mechanism are combined to create gene regulatory circuits with complex functions. Our primary experimental systems are two E. coli bacteriophages, lambda and 186. These temperate phages can replicate their genomes using alternative developmental pathways, lysis and lysogeny, and are some of the simplest organisms to make developmental decisions. Despite their relative simplicity, the phage systems combine a wide range of gene control mechanisms in complex ways and have many lessons to teach us.. The phage systems have been the springboard for my particular interests in DNA looping, molecular traffic on DNA and epigenetics.. DNA loops are created when proteins bound to ...
The DNA in its circular form contains 48,502 base-pairs...Bacteriophage lambda DNA in its circular form contains 48,502 base-pairs and codes for about 60 proteins. According to abstract phage Lambda has 46 clearly defined ORFs and another ~20 putative ORFs, totaling ~66ORFs (~60 proteins according to p.730 2nd paragraph). The number of bases is for the double stranded circular chromosome. 48,514 bp according to Lewin, Genes VIII, 2004, p.335 fig.12.11 ...
Bacteria and their viruses (phages) are abundant across diverse ecosystems and their interactions influence global biogeochemical cycles and incidence of disease. Problematically, both classical and metagenomic methods insufficiently assess the host specificity of phages and phage-host infection dynamics in nature. Here we review emerging methods to study phage-host interaction and infection dynamics with a focus on those that offer resolution at the single-cell level. These methods leverage ever-increasing sequence data to identify virus signals from single-cell amplified genome (SAG) datasets or to produce primers/probes to target particular phage- bacteria pairs (digital PCR and phageFISH), even in complex communities. All three methods enable study of phage infection of uncultured bacteria from environmental samples, while the latter also discriminates between phage-host interaction outcomes (e.g., lytic, chronic, lysogenic) in model systems. Together these techniques enable quantitative,
Usually bacteriophages lyse their hosts following infection, however a few so-called "temperate" phage undergo lysogeny. In lysogeny, the bacteriophage integrates its genome into that of its host. The phage, then, is replicated each time the bacterial cell divides. In the lysogenic state, the bacteriophage can have considerable influence over host physiology ...
Heineman et al. found that T7 phage could evolve the ability to discriminate between several host strains. T7wild-type "was independently adapted in two mixes of Escherichia coli strains: C with either B or K12. In both adaptations, C was the permissive host, while the other (B or K12, depending on the adaptation) aborted T7 infections due to deletion of a host gene needed for viral replication. Both adapted phages evolved to largely avoid the nonpermissive host but maintained a high adsorption rate to C." Notably the phage evolved the ability to discriminate via single amino acid substitutions in the tail fiber gene (used to bind host receptors ...
A stylized image of a bacteriophage. The capsid, tail, tail fibers, base plague and contractile shield are shown. - Stock Image F002/0293
First, related to the question at the beginning of the thread, I do not think you have to take this into account: Hes talking about bacteriophage, You just a sensitive strain of bacteria, the one used for propagating the phage would be good ...
Bacteriophages (phages) are probably the most abundant entities in nature, often exceeding bacterial densities by an order of magnitude. As viral predators
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నెల్లూరు: మనుబోలు మండలం బద్వేలు క్రాస్‌రోడ్డు దగ్గర కారు బోల్తా, ముగ్గురికి గాయాలు,కర్నూలు: 16 వ రోజు జగన్ ప్రజా సంకల్ప యాత్ర,రంగారెడ్డి: మైలార్‌దేవ్‌పల్లిలో కింగ్స్‌ కాలనీలో ముస్తఫా అనే వ్యక్తిపై దుండగుల కాల్పులు,కడప: జగన్ సీఎం అయితే తన ఆస్తులు పెరుగుతాయి..చంద్రబాబు సీఎంగా ఉంటే ప్రజల ఆస్తులు పెరుగుతాయి: మంత్రి సోమిరెడ్డి,సిరిసిల్ల: అన్ని గ్రామాల్లో కేసీఆర్ గ్రామీణ ప్రగతి ...
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The usefulness of a recombinant phage library depends on the ability to screen a large number of phage and identify the clone that carries the DNA sequence of interest
1.Experiments by the following scientists provided critical information concerning DNA. Fully describe 2 of these 3 classical experiments and indicate how each provided evidence for the chemical nature of the gene.a. Hershey and Chase- bacteriophage re...
Для выдвижного усилия поршня шприца в инъекционных насосах VIT-FIT и насосах VIT-FIT HP высокого давления установлен качественный швейцарский двигатель. Его новая технология обеспечивает высокий крутящий момент и на порядок более долгий срок службы. Для того чтобы перевести вращательное движение в линейное перемещение применяется шариковинтовая пара, выдерживающая большие механические нагрузки. Тем не менее, эти дорогостоящие компоненты играют решающую роль в обеспечении жесткости хода толкателя. Это важно для неизменной непульсирующей линейной ...
The objective of this study is to evaluate the safety and utility of bacteriophage phi X174 immunization as a tool to assess the immune competence of HIV-infected patients at different stages of disease in vivo, and to assess the impact of viral load levels and therapy-induced changes in viral load levels on the response to immunization with the neo-antigen bacteriophage phi X174. Bacteriophage phi X174 immunization is a method that has been in use for more than 25 years to assess the immunity of patients with various types of primary and secondary immunodeficiencies, including 48 HIV-infected patients. This is a prospective open-label, controlled study which will enroll 39 HIV-infected patients and 13 healthy volunteers, male or female with 18 years of age and over. The HIV-infected patients will be divided into 3 groups according to their CD4 cell count: less than 200 cells/mm(3), between 200 and 500 cells/mm(3) and greater than 500 cells/mm(3). After screening and a two week pre-study ...
Performance of the SBR in terms of commonly used physical, chemical and biological parameters has confirmed it to be a viable wastewater treatment option. This study further investigates the use of total coliforms, fecal coliforms and coliphages to evaluate the removal of selected microbiological indicators of potential pathogens by the SBR. Results from a pilot-scale SBR which received clarified sewage from a local treatment works treating a combined (domestic and industrial) sewage showed that increases in REACT time led to increases in the overall removal of the selected microorganisms. On average, up to 96% of total coliform and fecal coliform removals and up to 90% of coliphage removal was possible with the SBR operated with 2.5 h of REACT. During FILL, a long FILL (3h) resulted in reduction of coliforms while there was generally only a small reduction of coliphages. During REACT, a short REACT resulted in increase in selected microorganisms and the increase in coliphage numbers was ...
Phage therapy has also proven to be an effective therapeutic tool in fighting pathogenic strains of Escherichia coli, particularly in preventing the development of colibacillosis, which initially develops in the respiratory tract and air sacs and then takes the form of sepsis, causing considerable mortality in poultry.. Phage suspensions applied directly to the air sac in 3-day-old birds in a range of titres from 106 to 103 PFU to treat E. coli infections substantially reduced mortality rates to 5% and 25%, respectively. Similar results were obtained after inoculation of a bacteriophage suspension in the drinking water of birds at 1 week of age (103 or 104 PFU of bacteriophages per mL) followed by air sac challenge with 103 CFU of E. coli phages. Mortality was decreased to 25% and 5%, respectively. No mortality was observed in chickens treated with 108 PFU of an E. coli bacteriophage mixture [38]. Bacteriophages have also been shown to be highly effective in treating sepsis and meningitis in ...
Endosialidase (endo-N-acetylneuraminidase) is a tailspike enzyme of bacteriophages specific for human pathogenic Escherichia coli K1, which specifically recognizes and degrades polySia (polysialic acid). polySia is also a polysaccharide of the capsules of other meningitis- and sepsis-causing bacteria, and a post-translational modification of the NCAM (neural cell-adhesion molecule). We have cloned and sequenced three spontaneously mutated endosialidases of the PK1A bacteriophage and one of the PK1E bacteriophage which display lost or residual enzyme activity but retain the binding activity to polySia. Single to triple amino acid substitutions were identified, and back-mutation constructs indicated that single substitutions accounted for only partial reduction of enzymic activity. A homology-based structural model of endosialidase revealed that all substituted amino acid residues localize to the active site of the enzyme. The results reveal the importance of non-catalytic amino acid residues for ...
View DNA Rearrangements from BIOLOGY MCB2010 at Broward College. Examples : Integration of bacteriophage DNA into host bacterial chromosome Immunoglobulin and T Cell Receptor genes DNA rearrangements
Mouse anti-M13 phage coat protein g8p. Antibodies recognising M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. The monoclonal antibodies manufactured and supplied by Exalpha react with either the pIII (g3p) or pVIII (g8p) proteins of M13 filamentous bacteriophage. All antibodies are available in a purified format. The antibodies are fully validated and are suitable for a wide range of techniques including ...
Why would they need lysogeny-or even, how could they lysogenize? I cant speak for them all, but MS2 is a (+)-stranded ssRNA phage. On infection, the RNA can be directly translated into proteins that replicate the phage RNA and direct the synthesis of any capsid proteins required to form new phage particles. The replicase enzyme makes copies of both plus and minus strands, though the latter are only used as a template to make more (+) strand for both translation and packaging into phage. To lysogenize, there needs to be a DNA phase to the replication cycle and there isnt any such phase for MS2 ...
During PACE, host E. coli cells flow continuously into a fixed-volume vessel (a lagoon) containing a population of filamentous bacteriophages that encode a library of evolving proteins. The lagoon is continuously drained to a waste container after passing through an in-line luminescence monitor that measures expression from a gene III-luciferase cassette on the AP. Dilution occurs faster than cell division but slower than phage replication. Each phage carries a protein-encoding gene to be evolved instead of a phage gene (gene III) that is required for infection. Phage encoding active library members trigger expression of gene III on the AP in proportion to the desired activity and consequently produce infectious progeny. Phage encoding less active library members produce fewer infectious progeny and are lost by dilution. From: Nat. Chem. Biol. 10, 216-222 (2014). PDF. ...
The number of successful propagations/isolations of soil-borne bacteriophages is small in comparison to the number of bacteriophages observed by microscopy (great plaque count anomaly). As one...
1AE3: Analyses of the stability and function of three surface mutants (R82C, K69H, and L32R) of the gene V protein from Ff phage by X-ray crystallography.
Bacteriophages infecting bacteria, computer illustration. A bacteriophage, or phage, is a virus that infects bacteria. It consists of an icosahedral (20-sided) head, which contains the genetic material, a tail and tail fibres, which fix it to a specific receptor site on the bacterium. The tail injects the genetic material into the bacterium, and the cellular machinery of the bacterium is used to produce more copies of the virus. - Stock Image F016/7885
Proteome IDi ,p>The proteome identifier (UPID) is the unique identifier assigned to the set of proteins that constitute the ,a href="http://www.uniprot.org/manual/proteomes_manual">proteome,/a>. It consists of the characters UP followed by 9 digits, is stable across releases and can therefore be used to cite a UniProt proteome.,p>,a href=/help/proteome_id target=_top>More...,/a>,/p> ...
Help your students understand the connection between bacteriophages and human disease. This scholarly overview explores how bacteriophages have helped and hindered humans in their quest to overcome certain diseases. Use it as assigned reading or to kick off a classroom discussion.
In article ,1995Jan4.145054.1 at icbr2.ifas.ufl.edu,, aris at icbr2.ifas.ufl.edu says: , ,Dear Colleagues, , , We are interested in using PCR to amplify inserts from lambda ,gt11, and are familiar with doing this using purified lambda DNA as ,template. Can inserts be amplified without purifying the lambda DNA? ,If so, and suspensions of lambda phage particles in SM buffer can be ,used in PCR, how is this done? How much plate or liquid lysate is ,needed? Is a published method(s) available? Related comments and ,anecdotes would be appreciated. , ,Thanks, , ,John Aris ,aris at icbr.ifas.ufl.edu , , Hi, hope this helps - did it myself successfully several times: You can use a phage suspension as template, basically no matter how you got it - just eluted from the topagarose plug, from a plate lysate, or a liquid lysate. The higher the concentration, the better. If you get 100.000 or more phage particles for your PCR rxn, it should be enough. Use as little suspension as possible to get the right ...
Bacteriophage are viruses that infect bacteria. They were discovered independently by Frederick W. Twort in England in 1915 and by Felix d Herelle
For those molecular geneticists out there, you will appreciate the new discovery of using a genetically modified M13 phage as a source for making hydrogen fuel out of water! The M13 bacteriophage is often used in molecular genetics work as a cloning vector. The phage contains a single strand circular DNA genome of 6407 nucleotides…
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Bacteriophage phig1e Cng protein: a phage phi gle protein; amino acid sequence in first source; do not confuse with CNG channel (rod)
Research has suggested that bacteriophages derived and manipulated from ExPEC reservoirs are capable of combating infections caused by E.coli superbugs.
Postic, B., and M. Finland. 1961. Observations on bacteriophage typing of Pseudomonas aeruginosa. J. Clin. invest. 40:2064-2075. ...
Postic, B., and M. Finland. 1961. Observations on bacteriophage typing of Pseudomonas aeruginosa. J. Clin. invest. 40:2064-2075. ...
Host factor for Q beta, Host factor for Q beta, Host factor for Q beta, Host factor for Q beta, Host factor for Q beta, Host factor for Q beta, 5-R(*AP*UP*UP*UP*UP*UP*G)-3 ...
Although I am fully convinced of the truth of the views given in this volume, I by no means expect to convince experienced naturalists whose minds are stocked with a multitude of facts all viewed, during a long course of years, from a point of view directly opposite to mine. It is so easy to hide our ignorance under such expressions as "plan of creation," "unity of design," etc., and to think that we give an explanation when we only restate a fact. Any one whose disposition leads him to attach more weight to unexplained difficulties than to the explanation of a certain number of facts will certainly reject the theory. ...
Phages in this cluster are generally temperate. (This does not, however, guarantee that every single phage will be able to form lysogens, as some may have had important lysogeny genes deleted.). ...
T gjitha p rgatesat tona jan p rmendur dhe p rshkruar n k to faqe p r nj q llim t vet m q ju t jeni t informuar p r aktivitetet e p rgjithashme t Alkaloid it, e jo si k shill p r p rdorim t ndonj p rgatese n nj situat t caktuar. P r fardo lloj k shille n lidhje me p rdorimin e drejt t p rgatesave tona, kontaktoni me mjekun tuaj am ose farmacistin dhe lexoni me kujdes prospektet p r m nyr n e p rdorimit, q ndodhet n do paketim ...
T gjitha p rgatesat tona jan p rmendur dhe p rshkruar n k to faqe p r nj q llim t vet m q ju t jeni t informuar p r aktivitetet e p rgjithashme t Alkaloid it, e jo si k shill p r p rdorim t ndonj p rgatese n nj situat t caktuar. P r fardo lloj k shille n lidhje me p rdorimin e drejt t p rgatesave tona, kontaktoni me mjekun tuaj am ose farmacistin dhe lexoni me kujdes prospektet p r m nyr n e p rdorimit, q ndodhet n do paketim ...
Guanfacin (Teneks, Intuniv) je simpatolitik. On je selektivni agonist α2A receptora. Ti receptori su koncentrisani u prefrontalnom korteksu, te guanfacin potencijalno može da poboljša sposobnost pažnje putem modulisanja postsinaptičkih α2A receptora.[1] Guanfacin snižava sistolni i dijastolni krvni pritisak putem aktivacije α-2a norepinefrinskih autoreceptora centralnog nervnog sistema, što dovodi do redukcije perifernog simpatetičkog odliva i stoga do redukcije perifernog simpatetičkog tona.[2] Njegove nuspojave su zavisne od doze. Pri dozama do 2 mg efekat suvih usta se praktično ne javlja.[3] ...
Due to the increased prevalence of resistant bacterial isolates which are no longer susceptible to antibiotic treatment, recent emphasis has been placed on finding alternative modes of treatment of wound infections. Bacteriophage have long been investigated for their antimicrobial properties, yet the utilization of phage therapy for the treatment of wound infections relies on a suitable delivery system. Poly(N-isopropylacrylamide) (PNIPAM) is a thermally responsive polymer which undergoes a temperature dependent phase transition at a critical solution temperature. Bacteriophage K has been successfully formulated with PNIPAM nanospheres copolymerized with allylamine (PNIPAM-co-ALA). By utilizing a temperature responsive polymer it has been possible to engineer the nanospheres to collapse at an elevated temperature associated with a bacterial skin infection. The nanogels were reacted with surface deposited maleic anhydride in order to anchor the nanogels to non-woven fabric. Bacteriophage ...
The effect of the cellular level of RecA protein on the ability of E. coli K12 bacteria to (i) survive UV-irradiation (ii) promote UV-reactivation of UV-damaged phage lambda (iii) induce prophage lambda was determined in bacterial mutants with discrete increasing levels of RecA protein. The various levels of RecA protein were obtained by combining lexA and recA alleles. Except for the double mutant lexA3 recAo98, whose repair ability was 25% less than that observed in wild type bacteria, bacterial survival was proportional to the level of RecA protein measured after 90 min of incubation. In lexA3 recAo98 bacteria, RecA protein, at a constitutive high basal level, failed to compensate totally for the lack of LexA repressor cleavage; UV-reactivation of UV-damaged phage lambda was not restored; yet, prophage lambda was induced with 35% efficiency. Efficient UV-induction of prophage lambda is linked to the induction of lexA-controlled host processes that repair the UV-damaged prophage.
TY - JOUR. T1 - High efficiency, restriction-deficient in vitro packaging extracts for bacteriophage lambda DNA using a new E.coli lysogen. AU - Gunther, Edward. AU - Murray, Noreen E.. AU - Glazer, Peter M.. PY - 1993/8/11. Y1 - 1993/8/11. UR - http://www.scopus.com/inward/record.url?scp=0027236017&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027236017&partnerID=8YFLogxK. U2 - 10.1093/nar/21.16.3903. DO - 10.1093/nar/21.16.3903. M3 - Article. C2 - 8396240. AN - SCOPUS:0027236017. VL - 21. SP - 3903. EP - 3904. JO - Nucleic Acids Research. JF - Nucleic Acids Research. SN - 0305-1048. IS - 16. ER - ...
Food treated with bacteriophage, as to reduce or prevent the growth of undesirable bacteria. The food treated comprises: a food product; a first, fatty or waxy coating layer on the food product; and a second coating layer comprising one or more bacteriophage strains, wherein the fatty or waxy coating layer is distinct from the coating layer comprising the one or more bacteriophage strains. The food may be coated with bacteriophage and rubbed to distribute the phage on the food surface. The food may be preferably a pet food. The food may be coated in two or more layers. A process for treating food with bacteriophage comprises contacting the food with the bacteriophage and rubbing the coated food surface. The coating and/or rubbing may be performed in vibratory conveyor.
The aim of this work was to verify the possibility of applying affinity chromatography in bacteriophage purification, from the perspective of therapeutic purposes. Elution profiles of phages modified with specific affinity motifs (Figures 3,4,5 and 6) show substantially higher phage concentration in elution fractions compared to final washing samples. This indicates binding of modified phages to the affinity resins and effective elution with standard competitive agents. Thus, affinity tags can be successfully incorporated into the T4 phage capsid by the in vivo phage display technique and they strongly elevate bacteriophage affinity to a specific resin. Non-specific binding was also observed: unmodified phages or those modified with the non-specific tag were eluted with the titre 104-105 pfu/ml. Nevertheless, the unspecific binding is 102-105 times weaker than the specific one and importantly it does not interfere with the aim of preparation of purified anti-bacterial active bacteriophages for ...
Numerous authors have noted the difficulty in obtaining mutants of E. coli B that are resistant to bacteriophage T2 using standard procedures of plating large numbers of cells in the presence of excess phage. Yet, T2-resistant mutants appear in continuous culture at rates inconsistent with this difficulty. This paradoxical result derives from the fact that resistance to T2 usually arises as a consequence of two nonindependent mutations. Mutant bacteria resistant to phage T4 are very common and increase rapidly in continuous culture with phage T2 owing to an approximate halving of the rate at which T2 adsorbs to and kills these partially resistant mutants. The rate at which these partially resistant mutants then give rise to fully resistant mutants is approximately two orders of magnitude higher than the rate obtained by direct selection. These results are consistent with biochemical evidence that T2 adsorption to E. coli B involves both the bacterial lipopolysaccharide (to which phage T4 ...
We have examined the impact of DNA heterologies on the packaging of lambda DNA in vitro. Heterology-containing DNA molecules were constructed by denaturing and reannealing a mixture of DNA from cI+ phage and DNA front phage carrying small insertion or deletion mutations in the cI gene. We found that molecules with heterologies of up to 19 base pairs (bp) can be packaged as viable heterozygous phage with approximately the same efficiency as molecules with a base pair mismatch. In contrast, with a heterology of 26-bp heterozygous plaque formers are rare. In principle, the absence of cI heterozygotes among packaged phage may be due either to a failure to encapsulate the DNA or a failure to inject the packaged DNA on infection. Southern blot analysis of DNA isolated from packaged phage indicates that DNA harboring a 26-bp heterology is almost completely absent in packaged phage. Thus, an upper limit has been established for the size of heterology that can be accommodated by the packaging apparatus ...
Bacteriophages, the viruses that infect bacteria, are the most abundant biological entities in the biosphere and play a key role in global biogeochemical cycling. All T4-type bacteriophage isolates tested so far have a conserved genetic module that encodes the virion components including gene 23 (g23), the major capsid protein. Molecular analysis of the g23 sequence revealed a remarkable level of diversity of T4-type bacteriophages isolated from rice straw and surface soil in a Japanese rice field. It was found that g23 sequences obtained from the rice field were quite distinctive from those obtained in marine environments. Phylogenetic analysis showed that most of these g23 sequences belonged to two novel subgroups of T4-type bacteriophages, although some of them were related to well-studied subgroups of T4-type bacteriophages, such as marine cyanophage isolates of exoT-evens. ...
This chapter focuses on the doublestranded DNA (dsDNA) phages, and especially on the temperate phages. While virulent phages certainly perform transduction and engage in evolutionary sparring with their hosts and so influence their evolution, the chapter focuses mainly on the complex interactions of temperate phages with their hosts. Bacteriophages may thus have contributed to the current compact nature of bacterial genomes. The approximately 100 currently published bacterial genome complete nucleotide sequences, and about 285 prophages are related to known bacteriophages. Of the more than 280 prophages in the currently sequenced bacterial genomes, only a few are known to be fully functional bacteriophages. There are two rather complex types of genetic entity in which this appears to have happened: the phage tail-like bacteriocins and the gene transfer agents. To date, protection from other phages and disease virulence factors are the lysogenic conversion genes that have been discovered and studied in
Define phage. phage synonyms, phage pronunciation, phage translation, English dictionary definition of phage. n. A bacteriophage. n short for bacteriophage n. bacteriophage. a combining form meaning
Emory University biophysicists have experimentally demonstrated, for the fist time, how the nonspecific binding of a protein known as the lambda repressor, or C1 protein, bends DNA and helps it close a loop that switches off virulence. The researchers also captured the first measurements of that compaction.. Their results, published in Physical Review E, support the idea that nonspecific binding is not so random after all, and plays a critical role in whether a pathogen remains dormant or turns virulent.. "Our findings are the first direct and quantitative determination of non-specific binding and compaction of DNA," says Laura Finzi, an Emory professor of biophysics whose lab led the study. "The data are relevant for the understanding of DNA physiology, and the dynamic characteristics of an on-off switch for the expression of genes.". C1 is the repressor protein of the lambda bacteriophage, a virus that infects the bacterial species E. coli, and a common laboratory model for the study of gene ...
http://www.ibioseminars.org/ Bacteriophage, viruses that specifically infect bacteria, are, by far, the majority of all biological entities in the biosphere....
Former Brazilian president Luiz Inacio Lula da Silva, known popularly by the people of Brazil as Lula, has been diagnosed with throat cancer on Saturday and is scheduled to begin chemotherapy next week.
I also thought it was poetic. The bacteriophage has a long tail so I thought it "Longs to be inside a bacteria." Some people say, "I long to be with her" or "I long to be a doctor." The phrase is meant to express a desire. The bacteriophage wants to infect a host and that is why its tail is so long ...
J:134435 Anselme I, Laclef C, Lanaud M, Ruther U, Schneider-Maunoury S, Defects in brain patterning and head morphogenesis in the mouse mutant Fused toes. Dev Biol. 2007 Apr 1;304(1):208-20 ...
A virus that infects bacteria is called a bacteriophage, or simply a phage. Phages are composed of protein and nucleic acid, either DNA or...
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Less use of the tongs at all. I rarely use them now, but Id like to almost eliminate their use except for high in the tree grabs of species I have yet to record ...
An aerobic biofilter system was studied to assess its effectiveness for reducing enteric microbial indicators in flushed swine wastewater under different seasonal conditions. A laboratory-scale, low-pressure UV collimated beam apparatus was used to investigate the effectiveness of UV irradiation for inactivating enteric bacteria, coliphages, and bacterial spores in treated and untreated swine wastewater having unfiltered absorbances of 5 to 11 cm−1 and total suspended solids concentrations of 500 to 1200 mg/L. Fecal coliforms, Escherichia coli, enterococci, somatic coliphages, and male-specific coliphages were reduced by 97 to 99% in the biofilter system when reactor water temperatures were between 23 and 32 °C. Salmonella were reduced by 95 to 97% when water temperatures were 17 to 32 °C. Of the six microbial indicators studied, Clostridium perfringens spores were typically reduced the least by the biofilter system. At an average absorbed UV irradiation dose of 13 mJ/cm 2 , maximum ...
Some mammalian carcinogens and their metabolites affect the viability of Salmonella typhimurium strains, as indicated by a decrease in colony formation, and also induce prophage. We determined the minimum concentration required for prophage induction and the maximum prophage induction frequency for each carcinogen. The latter value was determined by the ratio of the number of induced phage particles relative to that of spontaneously induced phage particles in the controls. This value is constant for each carcinogen, regardless of its concentration. Since damage of the bacterial genome results in prophage induction, the reactivity of each compound with the genome may be indicated by the minimum concentration required for prophage induction and the maximum frequency of prophage induction. Carcinogens unable to affect bacterial viability are also unable to induce prophage. Failure to induce prophage indicates a requirement for metabolic activation by mammalian enzymes. Interaction of these ...
In the article, Dr Mohammed and Dr Millard discuss the issue of antibiotic resistance and warned that we are on the cusp of a "post-antibiotic era". They outlined how viruses that infect and kill bacteria, known as bacteriophages (phages), may be a possible alternative to antibiotics. They wrote: "Bacteriophages can survive in many environments, including deep sea trenches and the human gut. While phages are efficient killers of bacteria, they dont infect human cells and are harmless to humans.". They added: "Lytic bacteriophages are preferred for treatment because they dont integrate into the bacterial hosts chromosome. But it is not always possible to develop lytic bacteriophages that can be used against all types of bacteria.". Dr Mohammed and Dr Millard indicated that for bacteriophages to become commonplace for curing infections, there needs to be more research into the area of how bacteriophages interact with bacteria.. Read the full article on The Conversations website.. ...
Use of bacteriophage T7 displayed peptides for determination of monoclonalantibody specificity and biosensor analysis of the binding reaction. ...
Phage transduction is used to move selectable genetic markers from one "donor" strain to another "recipient" strain. Nat Sternberg, among others, pioneered the use of phage P1 to move genetic elements in E. coli and the use of the Cre/Lox system from P1 for controlled recombination. Today, phage P1 is commonly used as a transducing agent because it is a generalized tranducer (it can package random sections of the host chromosome instead of its own genome) giving rise to "transducing particles". P1vir is a mutant phage that enters the lytic cycle upon infection (ensuring replication and lysis). During the replication and lysis of the phage in a culture of bacteria, a small percentage of the phage particles will contain a genome segment that contains your gene of interest. P1 packages approximately 90 kb of DNA, so you can transduce genes that are linked to a selectable marker. Once a phage population has been generated from a donor host, the phage are used to infect a recipient host. Most of the ...
The definition of a protein coding domain that we used here is a contiguous stretch of DNA that, when transcribed, produces an mRNA that specifies the amino acid sequence of a protein. The T7 protein coding domains were first characterized by the isolation and analysis of randomly generated amber mutants. Nineteen genes were identified by mapping mutants that disrupt T7 DNA synthesis, particle maturation, and lysis (Studier, 1969; Haussman & Gomez, 1967; Haussman & LaRue, 1969). Two additional genes, T7 DNA ligase and protein kinase, were isolated via loss of function and deletion, respectively (Masamune et al, 1971); the genetic analysis of ligase and kinase mutants was carried out using mutant host strains that do not support the growth of ligase or kinase defective phage (Studier, 1969). Up to thirty T7 proteins were observed by pulsing phage-infected cells with radioactive amino acids (Studier & Maizel, 1969; Studier, 1973). Further experiments, such as electrophoretic mobility shifts of ...
Plays an essential role in phage DNA replication by participating in the removal of DNA-linked RNA primers. Participates also in T7 DNA packaging, host DNA degradation and phage genetic recombination.
(2005) Matsuda et al. Surgery. Background. Lysis-deficient (LyD) bacteriophages (phages) kill bacteria without endotoxin (Et) release. This may minimize systemic cytokine responses and limit inflammation in bacterial sepsis. We ...
InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool.
Specialized transduction occurs only in some temperate phages. But specialized transduction is an extremely efficient gene transfer mechanism.. In some occasions, DNA from a specific region of the host chromosome is integrated directly into the virus genome-usually replacing some viral genes. The resulting defective transducing phage (temperate phage) particles now have bacterial DNA as a part of genome.. To understand the process of specialized transduction, you must first be aware about lytic cycle of Bacteriophage.. Lets contrast between normal lysogenic cycle and mechanism of transduction. ...
Download this Blue Bacteriophage Plaques video now. And search more of the webs best library of royalty-free stock video footage from iStock.
Bacteriophages benefit to human health and are useful in numerous other fields. Focus on this biological microentity discovered at the end of 19th century
The research group led by Erez et al began their work by testing the hypothesis that bacteria secrete communication molecules to alert other bacteria of phage infection, but what they ended up finding was arguably much more interesting. They began their series of experiments by simply growing bacteria in liquid media with and without bacteriophages (see the figure to the right). They let the mixture sit long enough for the phages to infect their bacterial hosts for a couple of replication cycles (3 hours), and then removed all of the bacteria and phages from the liquid by filtration. At this point, if there was a signaling molecule released during the infection, it would still be in the media even though the phages and bacteria were removed. Additionally, if there was a signaling molecule released during the phage infection period, repeating an infection in that same media would result in altered growth patterns (for example, less bacteria killed when the molecule is present). As it turns out, ...
In Chapter 1, "The Old Man of La Chapelle: The Patriarch of Paleo," author Lydia Pyne explains the publics evolving conception of the first complete Neanderthal skeleton found and described by scientists.. 1 Comment. ...
Ciclo Celular Se presenta en Es una secuencia de sucesos que conducen primeramente al crecimiento de la célula y posteriormente a la división en células hijas Células procariontes Células eucariontes Fisión binaria Cuando la célula se divide la información genética contenida en su ADN debe duplicarse de manera precisa y luego las copias se transmiten a cada célula hija. La division la encontramos en dos etapas ...
) can release it, causing proliferation of new phages via the lytic cycle. Lysogenic cycles can also occur in eukaryotes, although the method of incorporation of DNA is not fully understood.Mixed cyclesFollowing are some types of viruses that…
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Scaldis Amber is a Belgian Strong Pale Ale style beer brewed by Brasserie Dubuisson Frères sprl in Leuze-Pipaix, Belgium. 3.86 average with 716 ratings, reviews and opinions.
With its capacity for anaerobic methane oxidation and denitrification, the bacterium Methylomirabilis oxyfera plays an important role in natural ecosystems. Its unique physiology can be exploited for more sustainable wastewater treatment technologies. However, operational stability of full-scale bioreactors can experience setbacks due to, for example, bacteriophage blooms. By shaping microbial communities through mortality, horizontal gene transfer, and metabolic reprogramming, bacteriophages are important players in most ecosystems. Here, we analyzed an infected Methylomirabilis sp. bioreactor enrichment culture using (advanced) electron microscopy, viral metagenomics and bioinformatics. Electron micrographs revealed four different viral morphotypes, one of which was observed to infect Methylomirabilis cells. The infected cells contained densely packed ~55 nm icosahedral bacteriophage particles with a putative internal membrane. Various stages of virion assembly were observed. Moreover, during ...
TY - JOUR. T1 - Models of bacteriophage DNA packaging motors. AU - Serwer, Philip. PY - 2003/3/1. Y1 - 2003/3/1. N2 - An ATP-dependent motor drives a DNA genome into a bacteriophage capsid during morphogenesis of double-stranded DNA bacteriophages both in vivo and in vitro. The DNA molecule enters the capsid through a channel in the center of a symmetric protein ring called a connector. Mechanisms in two classes have been proposed for this motor: (1) An ATP-driven rotating connector pulls a DNA molecule via serial power strokes. (2) The connector rectifies DNA motion that is either thermal, biased thermal, or oscillating electrical field-induced (motor-ratchet hypothesis). Mechanisms in the first class have previously been proposed to explain the detailed structure of DNA packaging motors. The present study demonstrates that the motor-ratchet hypothesis also explains the current data, including data in the following categories: biochemical genetics, energetics, structure, and packaging ...
Amber mutations were introduced into every codon (except the initiating AUG) of the bacteriophage T4 lysozyme gene. The amber alleles were introduced into a bacteriophage P22 hybrid, called P22 e416, in which the normal P22 lysozyme gene is replaced by its T4 homologue, and which consequently depends upon T4 lysozyme for its ability to form a plaque. The resulting amber mutants were tested for plaque formation on amber suppressor strains of Salmonella typhimurium. Experiments with other hybrid phages engineered to produce different amounts of wild-type T4 lysozyme have shown that, to score as deleterious, a mutation must reduce lysozyme activity to less than 3% of that produced by wild-type P22 e416. Plating the collection of amber mutants covering 163 of the 164 codons of T4 lysozyme, on 13 suppressor strains that each insert a different amino acid substitutions at every position in the protein (except the first). Of the resulting 2015 single amino acid substitutions in T4 lysozyme, 328 were found to

Coliphage - WikipediaColiphage - Wikipedia

A coliphage is a type of bacteriophage that infects Escherichia coli. Examples include Bacteriophage lambda and Leviviridae. ... Coliphages at the US National Library of Medicine Medical Subject Headings (MeSH). ...
more infohttps://en.wikipedia.org/wiki/Coliphage

Replication of coliphage lambda DNA.  - PubMed - NCBIReplication of coliphage lambda DNA. - PubMed - NCBI

Replication of coliphage lambda DNA.. Taylor K1, Wegrzyn G.. Author information. 1. Department of Molecular Biology, University ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/7669336?dopt=Abstract

E. coli phage vector M13tg130 - complete.E. coli phage vector M13tg130 - complete.

Misc.Comments : These data and their annotation were supplied to GenBank by Will Gilbert under the auspices of the GenBank Currator Program. Assembled from M13mp7 and M13tg130-Polylinker by F. Pfeiffer. M13 is not lytic; the phage extrude through the cell wall. The replicative RF I form in the cell is double-stranded and circular, and acts like a plasmid. The phage form outside the cell is linear and single-stranded. A series of deletions and replacements into the polylinker of M13mp7 creates the final polylinker. Amersham vector is 7195 bp ...
more infohttp://genome-www.stanford.edu/vectordb/vector_descrip/M13TG130.html

Coliphage T4<i>r</i>, Living, 5 mL |...Coliphage T4<i>r</i>, Living, 5 mL |...

This coliphage is supplied as filtered (sterile) peptone broth suspension of high relative infective titer (109 to 1011 plaque- ... Coliphage T4r, Living, 5 mL. Item # 124335 Coliphage T4 r , Living, 5 mL is rated 5.0 out of 5 by 3. ... This coliphage is supplied as filtered (sterile) peptone broth suspension of high relative infective titer (109 to 1011 plaque- ... This coliphage is supplied as filtered (sterile) peptone broth suspension of high relative infective titer in screw-cap vial. ...
more infohttps://www.carolina.com/bacteriophage-cultures/coliphage-t4r-living-5-ml/124335.pr

A bacterial virulence determinant encoded by lysogenic coliphage lambda.  - PubMed - NCBIA bacterial virulence determinant encoded by lysogenic coliphage lambda. - PubMed - NCBI

A bacterial virulence determinant encoded by lysogenic coliphage lambda.. Barondess JJ1, Beckwith J. ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/2144037?dopt=Abstract

Coliphage T4<i>r</i>llB, Deletion Mutation B, Living, 5 mL |...Coliphage T4<i>r</i>llB, Deletion Mutation B, Living, 5 mL |...

This coliphage is supplied as filtered (sterile) peptone broth suspension of high relative infective titer in screw-cap vial. ... Coliphage T4rllB, Deletion Mutation B, Living, 5 mL. Item # 124533 Online Only *bvseo_sdk, java_sdk, bvseo-4.0.0 ... This coliphage is supplied as filtered (sterile) peptone broth suspension of high relative infective titer in screw-cap vial. ... This coliphage is supplied as filtered (sterile) peptone broth suspension of high relative infective titer in screw-cap vial. ...
more infohttps://www.carolina.com/bacteriophage-cultures/coliphage-t4rllb-living-deletion-mutation-b-5-ml/124533.pr

E. coli phage vector lambda MGU2 - incomplete, cloning sites/loxP sit.E. coli phage vector lambda MGU2 - incomplete, cloning sites/loxP sit.

Misc.Comments : Deposited by: Ichiro N. Maruyama Restriction digests of the clone give the following sizes (kb): HindIII--24.1, 17.6; EcoRI--22.0, 20.0; BamHI--24.8, 16.9; XbaI--32.7,9.0; NotI--41.7. (ATCC staff) Vector useful for constructing cDNA libraries. Permits positive selection for inserts using the Spi- phenotype, and excision of phagemid by lox/cre site-specific recombination. [1] To prepare phagemid from lambdaMGU2, grow recombinants on a RecA- host expressing the Cre protein (E. coli 1046[pCRE1], ATCC 77368) and select for ampicillin resistance. The pMGU product is 4.185 kb. [1] The order of the major features in the cloning region of the lambda vector is: lambda J - SmaI - SalI - loxP - EcoRI - M13 ori - ampR - pMB1 ori - HindIII - 3gam/BamHI/5gam - XhoI - loxP - SalI - lambda N. [1] Inserts can be amplified using the following primers flanking the BamHI cloning site: upstream 5-AAGAGGCAGAACTGGCAG-3 and downstream 5-ATCGATGCATAGCGATTC-3. [1] Efficiency of phagemid recovery is ...
more infohttp://genome-www.stanford.edu/vectordb/vector_descrip/LAMMGU2.html

Simultaneous concentration of enterococci and coliphage from marine waters using a dual layer filtration system on...Simultaneous concentration of enterococci and coliphage from marine waters using a dual layer filtration system on...

Article Simultaneous concentration of enterococci and coliphage from marine waters using a dual layer filtration system. ... Simultaneous concentration of enterococci and coliphage from .... Simultaneous concentration of enterococci and coliphage from ... Seventeen different coliphages were isolated from the treated municipal wastewater. These coliphages and MS2 were treated with ... No comments were found for Simultaneous concentration of enterococci and coliphage from marine waters using a dual layer ...
more infohttps://www.environmental-expert.com/articles/simultaneous-concentration-of-enterococci-and-coliphage-from-marine-waters-using-a-dual-layer-filtra-76394

IJERPH | Free Full-Text | Effect of Temperature on the Survival of F-Specific RNA Coliphage, Feline Calicivirus, and...IJERPH | Free Full-Text | Effect of Temperature on the Survival of F-Specific RNA Coliphage, Feline Calicivirus, and...

Our aim was to determine which of these two organisms (coliphage or E. coli) was better at indicating norovirus survival under ... We compared the survival of F-specific RNA coliphage MS2, feline calicivirus, and E. coli in normal tap water and in tap water ... Keywords: D-value; F-specific coliphage; chlorination; Norovirus; water D-value; F-specific coliphage; chlorination; Norovirus ... We compared the survival of F-specific RNA coliphage MS2, feline calicivirus, and E. coli in normal tap water and in tap water ...
more infohttp://www.mdpi.com/1660-4601/2/3/442

Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and...Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and...

Coliphage and C. perfringens counts suggested that the total removal and inactivation was more than 7 log10 viable ... Three of nine finished water samples of 20,000 liters each revealed residual levels of somatic coliphages at 0.03, 0.10, and ... By using multiple regression, the somatic coliphage counts were the only explanatory variable for the human enteric virus ... Clostridium perfringens and somatic coliphages as indicators of the efficiency of drinking water treatment for viruses and ...
more infohttps://aem.asm.org/content/59/8/2418?ijkey=78377283803fdaa49b244e8c9b3474760406c4c9&keytype2=tf_ipsecsha

Analysis of the enzymatic cleavage (beta elimination) of the capsular K5 polysaccharide of Escherichia coli by the K5-specific...Analysis of the enzymatic cleavage (beta elimination) of the capsular K5 polysaccharide of Escherichia coli by the K5-specific...

The capsular K5 polysaccharide of Escherichia coli is the receptor of the capsule-specific coliphage K5, which harbors an ... of the capsular K5 polysaccharide of Escherichia coli by the K5-specific coliphage: reexamination.. P Hänfling, A S Shashkov, B ... of the capsular K5 polysaccharide of Escherichia coli by the K5-specific coliphage: reexamination. ... of the capsular K5 polysaccharide of Escherichia coli by the K5-specific coliphage: reexamination. ...
more infohttps://jb.asm.org/content/178/15/4747?ijkey=a6aceaf25d1c6b9d118f8a1d429045b81e7639c1&keytype2=tf_ipsecsha

Low-temperature T4-like coliphages vB EcoM-VR5, vB EcoM-VR7 and vB EcoM-VR20, Archives of Virology | 10.1007/s00705-010-0656-6 ...Low-temperature T4-like coliphages vB EcoM-VR5, vB EcoM-VR7 and vB EcoM-VR20, Archives of Virology | 10.1007/s00705-010-0656-6 ...

"Low-temperature T4-like coliphages vB_EcoM-VR5, vB_EcoM-VR7 and vB_EcoM-VR20, Archives of Virology" on DeepDyve, the largest ... Low-temperature T4-like coliphages vB_EcoM-VR5, vB_EcoM-VR7 and vB_EcoM-VR20. Low-temperature T4-like coliphages vB_EcoM-VR5, ... Low-temperature T4-like coliphages vB_EcoM-VR5, vB_EcoM-VR7 and vB_EcoM-VR20. Kaliniene, Laura; Klausa, Vytautas; Truncaite, ... A conserved genetic module that encodes the major virion components in both the coliphage T4 and the marine cyanophage S-PM2 ...
more infohttps://www.deepdyve.com/lp/springer_journal/low-temperature-t4-like-coliphages-vb-ecom-vr5-vb-ecom-vr7-and-vb-ecom-ZM0gd8aPOP

Recovery of somatic coliphages in wastewater and seawater samples in relation to bacterial indicator organisms and water...Recovery of somatic coliphages in wastewater and seawater samples in relation to bacterial indicator organisms and water...

Recovery of somatic coliphages in wastewater and seawater samples in relation to bacterial indicator organisms and water ... Coliphages persisted in all seawater samples collected. Different hydrochemical parameters of the water samples were measured ... Faiza A. Fattouh, Mohamed A. El Shenawy, Sahar W. Hassan; Recovery of somatic coliphages in wastewater and seawater samples in ... Recipient(s) will receive an email with a link to Recovery of somatic coliphages in wastewater and seawater samples in ...
more infohttps://iwaponline.com/aqua/article/53/3/183/30579/Recovery-of-somatic-coliphages-in-wastewater-and

Indicators - ColiphagesIndicators - Coliphages

Coliphages in guidelines and regulations. Phages infecting Bacteroides as markers of the source of fecal pollution ... 3.1 Coliphages. 3.2 Coliphages in guidelines and regulations. 3.3 Phages infecting bacteroides as markers of the source of ... 3.1 Coliphages. A coliphage is defined as a bacteriophage able to infect Escherichia coli. This bacterium is included among the ... F-specific coliphages, also named sexual coliphages, are those infecting E. coli through the sexual pili and comprise phages of ...
more infohttps://coliphages.com/index.php/indicators/

Use of non-linear mixed-effects modelling and regression analysis to predict the number of somatic coliphages by plaque...Use of non-linear mixed-effects modelling and regression analysis to predict the number of somatic coliphages by plaque...

The present study aimed to establish the kinetics of the appearance of coliphage plaques using the double agar layer titration ... Use of non-linear mixed-effects modelling and regression analysis to predict the number of somatic coliphages by plaque ... The study indicates that the traditional coliphage plating technique has a reasonable potential to provide results in a single ... Repeated measurements of the appearance of plaques of coliphages titrated according to ISO 10705-2 at different times were ...
more infohttps://iwaponline.com/jwh/article-abstract/15/5/706/28719/Use-of-non-linear-mixed-effects-modelling-and?redirectedFrom=fulltext

Association with Natural Organic Matter Enhances the Sunlight-Mediated Inactivation of MS2 Coliphage by Singlet Oxygen -...Association with Natural Organic Matter Enhances the Sunlight-Mediated Inactivation of MS2 Coliphage by Singlet Oxygen -...

MS2 coliphage, a surrogate for human enteric viruses, is inactivated by singlet oxygen (1O2) produced via sunlight-mediated ... Abstract: MS2 coliphage, a surrogate for human enteric viruses, is inactivated by singlet oxygen (1O2) produced via sunlight- ... Association with Natural Organic Matter Enhances the Sunlight-Mediated Inactivation of MS2 Coliphage by Singlet Oxygen Kohn, T. ... Home , Association with Natural Organic Matter Enhances the Sunlight-Mediated Inactivation of MS2 Coliphage by Singlet Oxygen ...
more infohttps://infoscience.epfl.ch/record/111398

Fate of coliforms and coliphages in the sequencing batch reactor (SBR) | ScholarBank@NUSFate of coliforms and coliphages in the sequencing batch reactor (SBR) | [email protected]

Fate of coliforms and coliphages in the sequencing batch reactor (SBR). Authors: Ng, W.J. Sim, G.S.. Ong, S.L. Ng, K.Y. ... Ng, W.J., Sim, G.S., Ong, S.L., Ng, K.Y., Ramasamy, M., Tan, K.N. (1993). Fate of coliforms and coliphages in the sequencing ... On average, up to 96% of total coliform and fecal coliform removals and up to 90% of coliphage removal was possible with the ... This study further investigates the use of total coliforms, fecal coliforms and coliphages to evaluate the removal of selected ...
more infohttp://scholarbank.nus.edu.sg/handle/10635/65586

Smartphone-Based
in-Gel Loop-Mediated Isothermal Amplification
(gLAMP) System Enables Rapid Coliphage MS2 Quantification in...Smartphone-Based in-Gel Loop-Mediated Isothermal Amplification (gLAMP) System Enables Rapid Coliphage MS2 Quantification in...

System Enables Rapid Coliphage MS2 Quantification in Environmental Waters ... Two R 2 gLAMP results System Enables Rapid Coliphage MS 2 Quantification ambient water RNA sample virus concentrations ... amplicon dot culture-based plaque assays water-quality analysis PRD pathogenic viruses Environmental Waters Model coliphages in ... Model coliphages (e.g., ΦX174, MS2, and PRD1) have been widely used as surrogates to study the fate and transport of pathogenic ...
more infohttps://figshare.com/collections/Smartphone-Based_in-Gel_Loop-Mediated_Isothermal_Amplification_gLAMP_System_Enables_Rapid_Coliphage_MS2_Quantification_in_Environmental_Waters/4101971

Comparative study of enteric viruses, coliphages and indicator bacteria for evaluating water quality in a tropical high...Comparative study of enteric viruses, coliphages and indicator bacteria for evaluating water quality in a tropical high...

Detection of Coliphages. Coliphages were detected from concentrated water samples using Escherichia coli K12 Hfr (ATCC) as the ... Coliphages have been shown to be complementary or equivalent to other indicators, therefore it is highly advisable to include ... Coliphages proved to be adequate faecal pollution indicators for the irrigation water studied. Viral presence in this tropical ... Positive samples for coliphages coincided more frequently with the positive samples for indicator bacteria than EV, or the ...
more infohttps://ehjournal.biomedcentral.com/articles/10.1186/1476-069X-8-49

Characterization of a T5-Like Coliphage, SPC35, and Differential Development of Resistance to SPC35 in Salmonella enterica...Characterization of a T5-Like Coliphage, SPC35, and Differential Development of Resistance to SPC35 in Salmonella enterica...

Characterization of a T5-Like Coliphage, SPC35, and Differential Development of Resistance to SPC35 in Salmonella enterica ... Characterization of a T5-Like Coliphage, SPC35, and Differential Development of Resistance to SPC35 in Salmonella enterica ... Characterization of a T5-Like Coliphage, SPC35, and Differential Development of Resistance to SPC35 in Salmonella enterica ... Characterization of a T5-Like Coliphage, SPC35, and Differential Development of Resistance to SPC35 in Salmonella enterica ...
more infohttps://aem.asm.org/content/77/6/2042/figures-only

Ribonucleic acid synthesis by Escherichia coli C 3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties...Ribonucleic acid synthesis by Escherichia coli C 3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties...

Ribonucleic acid synthesis by Escherichia coli C 3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties ... Ribonucleic acid synthesis by Escherichia coli C 3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties ... Ribonucleic acid synthesis by Escherichia coli C 3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties ... Ribonucleic acid synthesis by Escherichia coli C 3000/L after infection by the ribonucleic acid coliphage ZIK/1, and properties ...
more infohttp://www.biochemj.org/content/100/3/601

Assessment of Coliphage Surrogates for Testing Drinking Water Treatment Devices<...Assessment of Coliphage Surrogates for Testing Drinking Water Treatment Devices<...

Five different coliphages (MS-2, PRD1, ΦX-174, Qβ, and fr) were compared to the removal of poliovirus type 1 (LSc-2ab) by eight ... Five different coliphages (MS-2, PRD1, ΦX-174, Qβ, and fr) were compared to the removal of poliovirus type 1 (LSc-2ab) by eight ... Five different coliphages (MS-2, PRD1, ΦX-174, Qβ, and fr) were compared to the removal of poliovirus type 1 (LSc-2ab) by eight ... Five different coliphages (MS-2, PRD1, ΦX-174, Qβ, and fr) were compared to the removal of poliovirus type 1 (LSc-2ab) by eight ...
more infohttps://arizona.pure.elsevier.com/en/publications/assessment-of-coliphage-surrogates-for-testing-drinking-water-tre

Adelaide Research & Scholarship: Secretion in Escherichia coli & phage-display of recombinant insulin-like growth factor...Adelaide Research & Scholarship: Secretion in Escherichia coli & phage-display of recombinant insulin-like growth factor...

Secretion in Escherichia coli & phage-display of recombinant insulin-like growth factor binding protein-2. ... COS Cells; Animals; Cattle; Escherichia coli; Coliphages; Insulin-Like Growth Factor I; Insulin-Like Growth Factor II; Insulin- ...
more infohttps://drmc.library.adelaide.edu.au/dspace/handle/2440/11320

Complete Genome Sequence of Escherichia coli Phage vB EcoS Sa179lw, Isolated from Surface Water in a Produce-Growing Area in...Complete Genome Sequence of Escherichia coli Phage vB EcoS Sa179lw, Isolated from Surface Water in a Produce-Growing Area in...

Complete Genome Sequence of Escherichia coli Phage vB_EcoS Sa179lw, Isolated from Surface Water in a Produce-Growing Area in ... Complete genome sequence of Escherichia coli phage vB_EcoS Sa179lw, isolated from surface water in a produce-growing area in ... Complete Genome Sequence of Escherichia coli Phage vB_EcoS Sa179lw, Isolated from Surface Water in a Produce-Growing Area in ... Complete Genome Sequence of Escherichia coli Phage vB_EcoS Sa179lw, Isolated from Surface Water in a Produce-Growing Area in ...
more infohttps://mra.asm.org/content/6/27/e00337-18/article-info

Water and Wastewater Disinfection with Peracetic Acid and UV Radiation and Using Advanced Oxidative Process PAA/UVWater and Wastewater Disinfection with Peracetic Acid and UV Radiation and Using Advanced Oxidative Process PAA/UV

... and coliphages (such as virus indicators). Under the experimental conditions investigated, doses of 2, 3, and 4 mg/L of PAA and ... Besides that, total coliforms, E. coli, and coliphages were quantified in the wastewater and E. coli and coliphages in water. ... According to data presented in Table 4, the UV system was highly efficient in terms of E. coli and coliphages inactivation ... In relation to the inactivation of indicator microorganisms E. coli, TC, and coliphages, in both PAA tests and with all doses ...
more infohttps://www.hindawi.com/journals/ijp/2015/860845/
  • Five different coliphages (MS-2, PRD1, ΦX-174, Qβ, and fr) were compared to the removal of poliovirus type 1 (LSc-2ab) by eight different water treatment devices/systems using a general case and a challenge case (high organic load, dissolved solids, and turbidity) test water as defined by the USEPA. (elsevier.com)
  • Host strains (for example CB390) counting total coliphages have been developed (Guzman et al. (coliphages.com)
  • Coliphage and C. perfringens counts suggested that the total removal and inactivation was more than 7 log10 viable microorganisms. (asm.org)
  • Different hydrochemical parameters of the water samples were measured which showed variable correlation with coliphage counts. (iwaponline.com)
  • Biochemical oxygen demand, ammonium, total nitrogen, reactive phosphorus and total phosphorus showed highly significant direct correlation with somatic coliphages counts for all water source points in all seasons tested. (iwaponline.com)
  • Stepwise multiple regression analysis was performed to rank various parameters based on their effect on counts of somatic coliphages. (iwaponline.com)
  • Although this model is adequate, to simplify its applicability two linear models were developed to predict the numbers of coliphages reliably, using the PFU counts as determined by the ISO after only 3 hours of incubation. (iwaponline.com)
  • On average, up to 96% of total coliform and fecal coliform removals and up to 90% of coliphage removal was possible with the SBR operated with 2.5 h of REACT. (nus.edu.sg)
  • LSB-1, a reference coliphage strain, was classified as a member of the Podoviridae family with a cystic form (50 ± 5 nm diameter) and short tail (60 ± 5 nm long). (biomedcentral.com)
  • These coliphages and MS2 were treated with different dosages of chlorine in drinking water, and a combined chlorine/ultraviolet irradiation treatment for the chlorine-resistant coliphages. (environmental-expert.com)
  • Coliphage LSB-1 possesses a tailspike protein with endosialidase activity which is probably responsible for its specific enteroinvasive E.coli host range within the laboratory. (biomedcentral.com)
  • In summary, in using pass/fail criteria as recommended under USEPA guidelines for testing water treatment device/systems, the use of coliphages should be considered as an alternative to reduce cost and time of testing such devices/systems. (elsevier.com)
  • Historically, the F-specific phages strategy was developed since it was the common belief that somatic coliphages were replicating in the environment, and since pili are not synthesized below 32ºC, F-specific were less prone to replicate outside the gut. (coliphages.com)
  • Our aim was to determine which of these two organisms (coliphage or E. coli ) was better at indicating norovirus survival under the conditions of the experiment. (mdpi.com)