Colipase I and II, consisting of 94-95 and 84-85 amino acid residues, respectively, have been isolated from porcine pancreas. Their role is to prevent the inhibitory effect of bile salts on the lipase-catalyzed intraduodenal hydrolysis of dietary long-chain triglycerides.
An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.
FATTY ACIDS in which the carbon chain contains one or more double or triple carbon-carbon bonds.
A nodular organ in the ABDOMEN that contains a mixture of ENDOCRINE GLANDS and EXOCRINE GLANDS. The small endocrine portion consists of the ISLETS OF LANGERHANS secreting a number of hormones into the blood stream. The large exocrine portion (EXOCRINE PANCREAS) is a compound acinar gland that secretes several digestive enzymes into the pancreatic ductal system that empties into the DUODENUM.
Organic, monobasic acids derived from hydrocarbons by the equivalent of oxidation of a methyl group to an alcohol, aldehyde, and then acid. Fatty acids are saturated and unsaturated (FATTY ACIDS, UNSATURATED). (Grant & Hackh's Chemical Dictionary, 5th ed)
A group of fatty acids that contain 18 carbon atoms and a double bond at the omega 9 carbon.
Eighteen-carbon essential fatty acids that contain two double bonds.
A quaternary skeletal muscle relaxant usually used in the form of its bromide, chloride, or iodide. It is a depolarizing relaxant, acting in about 30 seconds and with a duration of effect averaging three to five minutes. Succinylcholine is used in surgical, anesthetic, and other procedures in which a brief period of muscle relaxation is called for.
The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.
Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
An organ of digestion situated in the left upper quadrant of the abdomen between the termination of the ESOPHAGUS and the beginning of the DUODENUM.
Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.
The branch of psychology which seeks to learn more about the fundamental causes of behavior by studying various psychologic phenomena in controlled experimental situations.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)
Unsaturated hydrocarbons of the type Cn-H2n, indicated by the suffix -ene. (Grant & Hackh's Chemical Dictionary, 5th ed, p408)
A chemical element having an atomic weight of 106.4, atomic number of 46, and the symbol Pd. It is a white, ductile metal resembling platinum, and following it in abundance and importance of applications. It is used in dentistry in the form of gold, silver, and copper alloys.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
Using an INTERNET based personal journal which may consist of reflections, comments, and often hyperlinks.
Lipids containing one or more phosphate groups, particularly those derived from either glycerol (phosphoglycerides see GLYCEROPHOSPHOLIPIDS) or sphingosine (SPHINGOLIPIDS). They are polar lipids that are of great importance for the structure and function of cell membranes and are the most abundant of membrane lipids, although not stored in large amounts in the system.
Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.
The physiological renewal, repair, or replacement of tissue.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
A genus of zygomycetous fungi of the family Mucoraceae, order MUCORALES, a common saprophyte and facultative parasite of mature fruits and vegetables. It may cause cerebral mycoses in diabetes and cutaneous infection in severely burned patients.
The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
The study of natural phenomena by observation, measurement, and experimentation.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Methods for determining interaction between PROTEINS.

Hydrolysis of emulsified mixtures of triacylglycerols by pancreatic lipase. (1/105)

Hydrolysis of the emulsified mixture of short-chain triacylglycerols by porcine pancreatic lipase in the presence of procolipase and micellar sodium taurodeoxycholate has been studied. Increase in the content of tributyrin and trioctanoin in the mixture with triacetin had highly cooperative effects on the formation of the interfacial lipase procolipase complex. Abrupt enhancement of the complex stability was observed in the presence of 0.4-0.6 mol mol-1 of tributyrin or 0.58 mol mol-1 of trioctanoin in the substrate phase. The affinity of lipase towards interfacially bound procolipase for the trioctanoin containing 0.07-0.42 mol mol-1 of triacetin was approximately three times higher than that for pure trioctanoin. The cooperative processes involved in complex formation did not contribute to the affinity of the interfacial lipase/(pro)colipase complex towards substrate molecules and its catalytic activity.  (+info)

Pancreatic lipase/colipase-mediated triacylglycerol hydrolysis is required for cholesterol transport from lipid emulsions to intestinal cells. (2/105)

This study tested the hypothesis that dietary cholesterol uptake by intestinal cells is dependent on the structure and composition of the lipid carriers in the extracellular milieu. In in vivo experiments with female C57BL/6 mice, cholesterol absorption from phospholipid/triacylglycerol emulsions was significantly reduced by administration of tetrahydrolipstatin, an inhibitor of pancreatic lipase. This inhibitor had no effect on the absorption of cholesterol from phospholipid vesicles. The importance of pancreatic-lipase-mediated triacylglycerol hydrolysis for cholesterol transport from emulsions to intestinal cells was confirmed by in vitro experiments with rat IEC-6 intestinal cells. Cellular uptake of cholesterol from emulsions with a phospholipid/triacylglycerol molar ratio of <0.3 could be stimulated by pancreatic lipase/colipase hydrolysis of the core neutral lipids. However, pancreatic lipase/colipase was ineffective in hydrolysing triacylglycerols in emulsions with a phospholipid/triacylglycerol molar ratio of >0.3. Phospholipase A2-mediated hydrolysis of the surface phospholipids was necessary prior to triacylglycerol hydrolysis in these phospholipid-rich emulsions and to the stimulation of cholesterol transport from these particles to IEC-6 cells. The data also revealed that minimal triacylglycerol hydrolysis was sufficient to significantly increase cholesterol transport from lipid emulsions to the intestinal cells. Thus the products of triacylglycerol hydrolysis, namely monoacylglycerol and non-esterified fatty acids, are key determinants in mediating cholesterol transport from lipid emulsions to intestinal cells. Taken together, these results support the hypothesis that remodelling of the surface and core components of lipid carriers is necessary prior to absorption of dietary cholesterol from the gastrointestinal tract.  (+info)

Colipase: structure and interaction with pancreatic lipase. (3/105)

Colipase is a small protein cofactor needed by pancreatic lipase for the efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising an active conformation and considerably increasing the overall hydrophobic binding site. Structural studies of the complex and of colipase alone have clearly revealed the functionality of its architecture. Interestingly, a structural analogy has recently been discovered between colipase and a domain in a developmental protein (Dickkopf), based on sequence analogy and homology modeling. Whether this structural analogy implies a common function (lipid interaction) remains to be clarified. Structural analogies have also been recognised between the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. These non-catalytic domains in the latter enzymes are important for interaction with membranes. It has not been established if these domains are also involved in eventual protein cofactor binding as is the case for pancreatic lipase.  (+info)

Critical role of micelles in pancreatic lipase activation revealed by small angle neutron scattering. (4/105)

In the duodenum, pancreatic lipase (PL) develops its activity on triglycerides by binding to the bile-emulsified oil droplets in the presence of its protein cofactor pancreatic colipase (PC). The neutron crystal structure of a PC-PL-micelle complex (Hermoso, J., Pignol, D., Penel, S., Roth, M., Chapus, C., and Fontecilla-Camps, J. C. (1997) EMBO J. 16, 5531-5536) has suggested that the stabilization of the enzyme in its active conformation and its adsorption to the emulsified oil droplets are mediated by a preformed lipase-colipase-micelle complex. Here, we correlate the ability of different amphypathic compounds to activate PL, with their association with PC-PL in solution. The method of small angle neutron scattering with D(2)O/H(2)O contrast variation was used to characterize a solution containing PC-PL complex and taurodeoxycholate micelles. The resulting radius of gyration (56 A) and the match point of the solution indicate the formation of a ternary complex that is similar to the one observed in the neutron crystal structure. In addition, we show that either bile salts, lysophospholipids, or nonionic detergents that form micelles with radii of gyration ranging from 13 to 26 A are able to bind to the PC-PL complex, whereas smaller micelles or nonmicellar compounds are not. This further supports the notion of a micelle size-dependent affinity process for lipase activation in vivo.  (+info)

Effect of high-fat diet, surrounding temperature, and enterostatin on uncoupling protein gene expression. (5/105)

Nonshivering thermogenesis induced in brown adipose tissue (BAT) during high-fat feeding is mediated through uncoupling protein 1 (UCP1). UCP2 is a recently identified homologue found in many tissues. To determine the role of UCP1 and UCP2 in thermoregulation and energy balance, we investigated the long-term effect of high-fat feeding on mRNA levels in mice at two different ambient temperatures. We also treated mice with the anorectic peptide enterostatin and compared mRNA levels in BAT, white adipose tissue (WAT), stomach, and duodenum. Here, we report that high-fat feeding at 23 degrees C increased UCP1 and UCP2 levels in BAT four- and threefold, respectively, and increased UCP2 levels fourfold in WAT. However, at 29 degrees C, UCP1 decreased, whereas UCP2 remained unchanged in BAT and increased twofold in WAT. Enterostatin increased UCP1 and decreased UCP2 mRNA in BAT. In stomach and duodenum, high-fat feeding decreased UCP2 mRNA, whereas enterostatin increased it. Our results suggest that the regulation of uncoupling protein mRNA levels by high-fat feeding is dependent on ambient temperature and that enterostatin is able to modulate it.  (+info)

Fats infused intraduodenally affect the postprandial secretion of the exocrine pancreas and the plasma concentration of cholecystokinin but not of peptide YY in growing pigs. (6/105)

In pigs, the spontaneous secretion of the exocrine pancreas and the release of cholecystokinin (CCK) and peptide YY (PYY) after intraduodenal infusion of fully saturated synthetic fats differing in chain length was studied. Growing pigs (n = 6) were prepared with pancreatic duct catheters, duodenal T-cannulas and catheters placed in the jugular vein. The pigs were fed 2 g/100 g body twice daily. Beginning with the morning feeding, a medium-chain triglyceride (MCT: glycerol tricaprylate), a long-chain triglyceride (LCT: glycerol tristearate) or saline was infused at a rate of 0.1 g/100 g body. Pancreatic juice was collected, beginning 1 h preprandially until 3 h postprandially. Blood samples were obtained 15 min preprandially and 15, 45, 90 and 150 min postprandially. The infusion of MCT evoked a change in the trend of the curve for the volume of secretion of pancreatic juice, lipase and colipase concentrations and outputs. The trend of the curve did not change over time for CCK and PYY. Differences between the trends of the curves for the saline and MCT treatment were observed for volume of secretion, protein output, lipase content and output, trypsin and colipase output. Differences in the trends of the curves between MCT and LCT were obtained for the outputs of protein, lipase and colipase. Plasma CCK levels were lower as a result of the MCT treatment compared with the saline and LCT treatments. The results suggest an immediate, distinguished response of the porcine exocrine pancreas to fats differing in chain length.  (+info)

Interactions of bile salt micelles and colipase studied through intermolecular nOes. (7/105)

Colipase is a small protein (10 kDa), which acts as a protein cofactor for the pancreatic lipase. Various models of the activated ternary complex (lipase-colipase-bile salt micelles) have been proposed using detergent micelles, but no structural information has been established with bile salt micelles. We have investigated the organization of sodium taurodeoxycholate (NaTDC) micelles and their interactions with pig and horse colipases by homonuclear nuclear magnetic resonance (NMR) spectroscopy. The NMR data supply evidence that the folding of horse colipase is similar to that already described for pig colipase. Intermolecular nuclear Overhauser effects have shown that two conserved aromatic residues interact with NaTDC micelles.  (+info)

The lipase C-terminal domain. A novel unusual inhibitor of pancreatic lipase activity. (8/105)

In vertebrates, dietary fat digestion mainly results from the combined effect of pancreatic lipase, colipase, and bile. It has been proposed that in vivo lipase adsorption on oil-water emulsion is mediated by a preformed lipase-colipase-mixed micelle complex. The main lipase-colipase binding site is located on the C-terminal domain of the enzyme. We report here that in vitro the isolated C-terminal domain behaves as a potent noncovalent inhibitor of lipase and that the inhibitory effect is triggered by the presence of micelles. Lipase inhibition results from the formation of a nonproductive C-terminal domain-colipase-micelle ternary complex, which competes for colipase with the active lipase-colipase-micelle ternary complex, thus diverting colipase from its lipase-anchoring function. The formation of such a complex has been evidenced by molecular sieving experiments. This nonproductive complex lowers the amount of active lipase thus reducing lipolysis. Preliminary experiments performed in rats show that the C-terminal domain also behaves as an inhibitor in vivo and thus could be considered a potential new tool for specifically reducing intestinal lipolysis.  (+info)

Buy our Recombinant Human Pancreatic Lipase Related Protein 2. Ab114929 is a protein fragment produced in Wheat germ and has been validated in WB, ELISA…
pancreatic lipase related protein 2: from human (hPLRP2) & guinea pig (gPLRP2); highly homologous to human pancreatic lipase (hPL), but its lipolytic activity is profoundly different from hPL; amino acid sequence has been determined
Although the structure of Human Pancreatic Lipase has been documented through the X-ray crystallography, the knowledge about the molecular rearrangement and dynamic equilibrium in the structure (particularly in the catalytic triad and lid domains) is very scanty. The structural fluctuations and conformational changes undergo by Human Pancreatic Lipase (HPL) with and without colipase were computationally investigated through molecular dynamics simulation technique using GROMACS 2018.4, MOE 2016.0801 and VMD softwares in order to gain insight into the complex transitions at different domains. The structural stability was revealed vis-a-vis Root Mean Square Deviation (RMSD) and Root Mean Square Fluctuations (RMSF) plots. The levels of compactness/folding and conformational changes of the protein were determined using Radius of gyration and secondary analysis respectively. Salt bridge analysis gives more ionic pairs interactions than experimentally determined results. Results show that though both proteins
Visceral adipose tissue plays a critical role in numerous diseases. While imaging studies often show adipose involvement in abdominal diseases, their outcomes may vary from being a mild self limited illness to one with systemic inflammation and organ failure. We therefore compared the pattern of visceral adipose injury during acute pancreatitis and acute diverticulitis to determine its role in organ failure. Acute pancreatitis-associated adipose tissue had ongoing lipolysis in the absence of adipocyte triglyceride lipase (ATGL). Pancreatic lipase injection into mouse visceral adipose tissue hydrolyzed adipose triglyceride and generated excess non-esterified fatty acids (NEFA), which caused organ failure in the absence of acute pancreatitis. Pancreatic triglyceride lipase (PNLIP) increased in adipose tissue during pancreatitis and entered adipocytes by multiple mechanisms, hydrolyzing adipose triglyceride and generating excessive NEFA. During pancreatitis, obese PNLIP knockout mice, unlike obese ...
Build: Wed Jun 21 18:33:50 EDT 2017 (commit: 4a3b2dc). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
Ota T, Suzuki Y, Nishikawa T, Otsuki T, Sugiyama T, Irie R, Wakamatsu A, Hayashi K, Sato H, Nagai K, Kimura K, Makita H, Sekine M, Obayashi M, Nishi T, Shibahara T, Tanaka T, Ishii S, Yamamoto J, Saito K, Kawai Y, Isono Y, Nakamura Y, Nagahari K, Murakami K, Yasuda T, Iwayanagi T, Wagatsuma M, Shiratori A, Sudo H, Hosoiri T, Kaku Y, Kodaira H, Kondo H, Sugawara M, Takahashi M, Kanda K, Yokoi T, Furuya T, Kikkawa E, Omura Y, Abe K, Kamihara K, Katsuta N, Sato K, Tanikawa M, Yamazaki M, Ninomiya K, Ishibashi T, Yamashita H, Murakawa K, Fujimori K, Tanai H, Kimata M, Watanabe M, Hiraoka S, Chiba Y, Ishida S, Ono Y, Takiguchi S, Watanabe S, Yosida M, Hotuta T, Kusano J, Kanehori K, Takahashi-Fujii A, Hara H, Tanase TO, Nomura Y, Togiya S, Komai F, Hara R, Takeuchi K, Arita M, Imose N, Musashino K, Yuuki H, Oshima A, Sasaki N, Aotsuka S, Yoshikawa Y, Matsunawa H, Ichihara T, Shiohata N, Sano S, Moriya S, Momiyama H, Satoh N, Takami S, Terashima Y, Suzuki O, Nakagawa S, Senoh A, Mizoguchi H, Goto Y, ...
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Colipase I and II, consisting of 94-95 and 84-85 amino acid residues, respectively, have been isolated from porcine pancreas. Their role is to prevent the inhibitory effect of bile salts on the lipase-catalyzed intraduodenal hydrolysis of dietary long-chain triglycerides ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Human samples. We compared acute diverticulitis to acute pancreatitis for the following reasons: (a) both are acute, (b) both occur in the same demographic, (c) both have radiographic (e.g., CT scans) and pathologic involvement of visceral fat, yet (d) their outcomes are very different, with a high prevalence of organ failure in pancreatitis versus diverticulitis. We wanted to understand the mechanism of higher organ failure in pancreatitis. All biochemical studies (October 2015 through May 2017) were done as part of a prospectively done observational study using consecutively procured patient waste residual samples remaining after drainage or after testing and processing had been completed on the tissue (in the pathology or microbiology departments) for medically indicated procedures independent of this research, and the residual was ready to be discarded. For example, the samples used for the current studies were residual material from pancreatic necrosis drained as per the revised Atlanta ...
SWISS-MODEL Template Library (SMTL) entry for 1pcn.1. SOLUTION STRUCTURE OF PORCINE PANCREATIC PROCOLIPASE AS DETERMINED FROM 1H HOMONUCLEAR TWO-AND THREE-DIMENSIONAL NMR
Fingerprint Dive into the research topics of Pectin at the oil-water interface: Relationship of molecular composition and structure to functionality. Together they form a unique fingerprint. ...
We present the behaviour of particles with chemical and geometrical anisotropy at a planar oil-water interface. We find that Janus cylinders with a small aspect ratio adopt an upright configuration, whereas the particles with a large aspect ratio exhibit both the upright and tilted configurations, which can
This entry represents a domain found in a variety of membrane or lipid associated proteins. It is known as the PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology) domain, is found in a variety of membrane or lipid associated proteins. Structurally, this domain forms a beta-sandwich composed of two sheets of four strands each [(PUBMED:10469604), (PUBMED:11985859), (PUBMED:11412104)]. The most highly conserved regions coincide with the beta-strands, with most of the highly conserved residues being buried within the protein. An exception to this is a surface lysine or arginine that occurs on the surface of the fifth beta-strand of the eukaryotic domains. In pancreatic lipase, the lysine in this position forms a salt bridge with the procolipase protein. The conservation of a charged surface residue may indicate the location of a conserved ligand-binding site. It is thought that this domain may mediate membrane attachment via other protein binding partners.. ...
This entry represents a domain found in a variety of membrane or lipid associated proteins. It is known as the PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology) domain, is found in a variety of membrane or lipid associated proteins. Structurally, this domain forms a beta-sandwich composed of two sheets of four strands each [(PUBMED:10469604), (PUBMED:11985859), (PUBMED:11412104)]. The most highly conserved regions coincide with the beta-strands, with most of the highly conserved residues being buried within the protein. An exception to this is a surface lysine or arginine that occurs on the surface of the fifth beta-strand of the eukaryotic domains. In pancreatic lipase, the lysine in this position forms a salt bridge with the procolipase protein. The conservation of a charged surface residue may indicate the location of a conserved ligand-binding site. It is thought that this domain may mediate membrane attachment via other protein binding partners.. ...
Catalog No :RS01Ab4753Organism- PigClonality :PolyclonalHost :RabbitConcentration :200ug/mlApplication :WB, ICC, IHC-P, IHC-F, ELISA
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Enzyme-linked immunoassay of mouse pancreatic lipase (PL) Kit instruction manual This reagent is for research purposes only: this kit is used to determine the activity of pancreatic lipase (PL) in mouse serum, plasma and related liquid samples. Experimental principle: This kit uses the double antibody sandwich method to determine the level of mouse pancreatic lipase (PL) in the specimen. Microporous plates were coated with purified pancreatic lipase (PL) antibody to prepare solid-phase antibodies. Pancreatic lipase (PL) was added to the monoclonal antibody-coated microwells, followed by HRP labeled pancreatic lipase (PL) antibody The combination forms an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with pancreatic lipase (PL) in the sample. The absorbance (OD value) ...
In molecular biology the PLAT domain is a protein domain that is found in a variety of membrane or lipid associated proteins. It is called the PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology) domain. The known structure of pancreatic lipase shows this domain binds to procolipase Pfam PF01114, which mediates membrane association. This domain is found in a variety of membrane or lipid associated proteins. It forms a beta-sandwich composed of two β-sheets of four β-strands each. ALOX12; ALOX12B; ALOX12P2; ALOX15; ALOX15B; ALOX5; ALOXE3; LIPC; LIPG; LOXHD1; LPL; PKD1; PKD1L1; PKD1L2; PKD1L3; PKDREJ; PNLIP; PNLIPRP1; PNLIPRP2; PNLIPRP3; RAB6IP1; van Tilbeurgh H, Egloff MP, Martinez C, Rugani N, Verger R, Cambillau C (April 1993). Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography. Nature. 362 (6423): 814-20. doi:10.1038/362814a0. PMID 8479519. Bateman A, Sandford R (1999). The PLAT domain: a new piece ...
Our long-term goal is to elucidate the molecular mechanisms of dietary fat digestion by pancreatic lipases. In this application, we will focus on the steps in l...
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Synonyms: related, related to Definition: being connected either logically or causally or by shared characteristics Usage: painting and the related arts; school-related activities; related to micelle formation is the...ability of detergent actives to congregate at oil-water interfaces Hypernyms: connected, affiliated, attached Definition: being joined in close association Usage: affiliated clubs; all art schools whether independent or attached to universities Hypernyms: age-related Definition: changing (increasing or decreasing) as an individuals age increases Hypernyms: bound up Definition: closely or inseparably connected or associated with Usage: his career is bound up with the fortunes of the enterprise Hypernyms: cognate Definition: having the same ancestral language Usage: cognate languages Hypernyms: cognate, connate Definition: related in nature Usage: connate qualities Hypernyms: coreferent Definition: related by sharing a symbolic link to a concrete object or an abstraction Usage: two ...
TY - JOUR. T1 - Behavior of plant-dairy protein blends at air-water and oil-water interfaces. AU - Hinderink, E.B.A.. AU - Sagis, L.M.C.. AU - Schroen, C.G.P.H.. AU - Berton-Carabin, C.C.. PY - 2020/8. Y1 - 2020/8. N2 - Recent work suggests that using blends of dairy and plant proteins could be a promising way to mitigate sustainability and functionality concerns. Many proteins form viscoelastic layers at fluid interfaces and provide physical stabilization to emulsion droplets; yet, the interfacial behavior of animal-plant protein blends is greatly underexplored. In the present work, we considered pea protein isolate (PPI) as a model legume protein, which was blended with well-studied dairy proteins (whey protein isolate (WPI) or sodium caseinate (SC)). We performed dilatational rheology at the air-water and oil-water interface using an automated drop tensiometer to chart the behavior and structure of the interfacial films, and to highlight differences between films made with either blends, or ...
In the presence of taurodeoxycholate, cis-unsaturated fatty acids increase porcine pancreatic lipase activity 15-fold at pH 7.5. This effect is saturable with a low proportion of fatty acid to substrate. The overall angle of the fatty acid, the position of its double bond and the presence of a carbo …
ALARCÓN, C. & CUBILLOS, L.A. & ACUÑA, E. (2011) Length-based growth, maturity and natural mortality of the cockfish Callorhinchus callorhynchus (Linnaeus, 1758) off Coquimbo, Chile. Environmental Biology of Fishes, 92 (1): 65-78 http://dx.doi.org/10.1007/s10641-011-9816-0. BABU, C. & RAMACHANDRAN, S. & VARGHESE, B.C. (2011) New record of sixgill sting ray Hexatrygon bickelli Heemstra and Smith, 1980 from south-west coast of India. Indian Journal of Fisheries, 58 (2): 137-139. BACHA, A.B. & KARRAY, A. & DAOUD, L. & BOUCHAALA, E. & ALI, M.B. & GARGOURI, Y. & ALI, Y.B. (2011) Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca. Lipids in Health and Disease, 10: 69, 1-7 http://dx.doi.org/10.1186/1476-511X-10-69. BLANCO-PARRA, M.P. & NIÑO-TORRES, C.A. (2011) Morphological abnormality in a diamond stingray, Dasyatis dipterura (Jordan & Gilbert, 1880) (Chondrichthyes: Dasyatidae), from the Gulf of California, Mexico Cahiers de Biologie Marine, 52 (3): ...
1ETH: Lipase activation by nonionic detergents. The crystal structure of the porcine lipase-colipase-tetraethylene glycol monooctyl ether complex.
Coypu (Myocastor coypus) at Galikos river. I have visited Galikos and Kalochori lagoon many times and Ive always wanted to see a coypu. Out of sheer luck the last I were there I saw one sitting atop some reeds ...
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Lipids, or fats, are digested and absorbed in the small intestine. In this lesson, you will learn how bile salts emulsify fat so pancreatic lipase...
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TY - JOUR. T1 - Effect of gastric lipase on turbidimetric and dry-film methods for measuring pancreatic lipase [1]. AU - Kazmierczak, S. C.. AU - Van Lente, F.. AU - Tetrault, G. A.. PY - 1992/12/1. Y1 - 1992/12/1. UR - http://www.scopus.com/inward/record.url?scp=0027098002&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027098002&partnerID=8YFLogxK. M3 - Letter. C2 - 1458599. AN - SCOPUS:0027098002. VL - 38. SP - 2555. EP - 2556. JO - Clinical Chemistry. JF - Clinical Chemistry. SN - 0009-9147. IS - 12. ER - ...
LIGAND BASED PHARMACOPHORE MODELING, VIRTUAL SCREENING AND MOLECULAR DOCKING STUDIES TO DESIGN NOVEL PANCREATIC LIPASE INHIBITORS
Obesity is a serious public health concern with an increasing prevalence worldwide. The diet contributes strongly to this problem and high-fat food leads to unhealthy conditions. Fat digestion is an energy intensive process as it requires hydrolysis to allow the body to profit from this nutrient. This additional energy expenditure is also present in a highly redundant hormonal regulation system for fat storage which is converted in not a readily accessible form (therefore, an upstream is required in order to tackle fat-related diseases). Due to the low water solubility of oils and fats, the lipase catalyzed reactions take place at a specific locus, the oil-water interface. Despite considerable efforts in the past the effects of the interfacial composition on lipase activity have been only qualitatively understood until recently. It has recently been undertaken a detailed study of the interfacial behavior of lipases, their substrates and products which have contributed to shed light into the mechanism of
Fat or lipids are heavily consumed in the average diet. Some of these fats are healthy and some are not quite as beneficial to our health. Regardless of the dietary nutritional value of the types of fat we consume, it is essential to properly break them down for our body to either utilize or remove. Dietary fats can be difficult to properly break down: first, because they are large molecules, and secondly, because they are not water soluble. ...
Talaromyces thermophilus lipase (TTL) was found to hydrolyze monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates presented in various forms to the enzyme. Different assay techniques were used for each substrate: pHstat with dioctanoyl galactolipid-bile salt mixed micelles, barostat with dilauroyl galactolipid monomolecular films spread at the air-water interface, and UV absorption using a novel MGDG substrate containing α-eleostearic acid as chromophore and coated on microtiter plates. The kinetic properties of TTL were compared to those of the homologous lipase from Thermomyces lanuginosus (TLL), guinea pig pancreatic lipase-related protein 2 and Fusarium solani cutinase. TTL was found to be the most active galactolipase, with a higher activity on micelles than on monomolecular films or surface-coated MGDG. Nevertheless, the UV absorption assay with coated MGDG was highly sensitive and allowed measuring significant activities with about 10 ng of enzymes, against 100
Bile salt-dependent lipase (or BSDL), also known as carboxyl ester lipase (or CEL) is an enzyme produced by the adult pancreas and aids in the digestion of fats. Bile salt-stimulated lipase (or BSSL) is an equivalent enzyme found within breast milk. BSDL has been found in the pancreatic secretions of all species in which it has been looked for. BSSL, originally discovered in the milk of humans and various other primates, has since been found in the milk of many animals including dogs, cats, rats, and rabbits. More than 95% of the fat present in human milk and in infant formulas is in the form of triacylglycerols (TG). In adults, TGs are thought to be broken down or hydrolyzed mainly by the colipase-dependent lipase enzyme. In the newborn, CDL activity in the duodenum is lower than in adults. Both BSDL and BSSL have a broad substrate specificity and, like CDL, are capable of hydrolyzing triacylglycerides (in addition to phospholipids, esters of cholesterol, and lipid-soluble vitamins). In ...
Semantic Scholar extracted view of [Effect of pancreatic lipase on the development of experimental atherosclerosis]. by I. P. Bondarenko
INTRODUCTION. Lipases (triacylglycerol acylhydrolases, EC3.1.1.3) are water-soluble enzymes that catalyze the hydrolysis of ester bonds of insoluble triacylglycerols, releasing free fatty acids, mono-or diacylglycerolin into the oil-water interface (Treichel et al., 2010). These enzymes catalyze a wide range of reactions such as hydrolysis, esterification, trans-esterification, alcoholysis, acidolysis and aminolysis (Joseph; Ramteke; Thomas, 2008), and may be of animal, microbial or plant origin, with variations in catalytic properties, and can be obtained by solid-state fermentation or submerged fermentation (Annibale et al., 2006; Rigo et al., 2010; Papagora; Roukas; Kotzekidou, 2013).. Lipases are an important group of enzymes with various applications in the food, chemical and pharmaceutical industry, besides having great interest for the treatment of effluents with high lipid content (Jeganathan; Nakhla; Bassi, 2007; Ertugrul; Donnez; Takac, 2007). The use of lipases in effluents with high ...
GASTROENTEROPATHY CHLORAMPHENICOL PROTOZOACIDES ENTEROPATHIC ENTERORRHAGIA ENTEROSALYL ENTEROSARINE ENTEROSEPTOL ENTEROSGEL ENTEROSIN ENTEROSOLUBILITY Procesals ENTEROSTATIN ENTEROTOXEMIA ENTEROTOXEMIA-VACCINE ENTEROTOXIGENIC ENTEROTOXIN ENTEROTOXIN-A-STAPH ENTEROTOXIN-B-HEAT-LABILE ENTEROTOXIN-HEAT-LABILE-E. 4 was higher than that at pH 5.
Pancreatic lipase. Hydrolyzes triacylglycerols (fats) into fatty acids and glycerol. Contains 19.6 USP units per milligram. Keep refrigerated.CAS: 9001-62-1Health Risk: 0Flammability: 0Reactivity: 1Synonym: Steapsin
The enzymes are not active when they are first made within the pancreas (otherwise they would digest the pancreas as well) but when they pass into the bowel they are activated by the juices in the bowel. The main enzymes are called amylase (which digests carbohydrates), trypsin (which digests protein) and lipase (which digests fats). The bile, which comes from the liver, is also very important for the digestion of fat because it acts like a soap and breaks up the fat into minute droplets so that the pancreatic lipase can digest it ...
The enzymes are not active when they are first made within the pancreas (otherwise they would digest the pancreas as well) but when they pass into the bowel they are activated by the juices in the bowel. The main enzymes are called amylase (which digests carbohydrates), trypsin (which digests protein) and lipase (which digests fats). The bile, which comes from the liver, is also very important for the digestion of fat because it acts like a soap and breaks up the fat into minute droplets so that the pancreatic lipase can digest it ...
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Lipids are important components in human nutrition; however, their increased intake contributes to the development of obesity and can lead to multiple long‐term complications. Pancreatic lipase (triacylglycerol acylhydrolase, EC 3.1.1.3) is a key enzyme for the absorption of dietary triglycerides. Interference with fat hydrolysis results in the reduced utilization of ingested lipids, therefore inhibition of lipases decreases fat absorption. Extracts from 106 species of medicinal plants, vegetables and fruits were screened for potential lipase inhibitory activity. p‐Nitrophenylpalmitate and 5‐bromo‐4‐chloro‐3‐indoxylpalmitate were used as substrates in an in vitro test with crude porcine pancreatic lipase. Bearberry (Arctostaphylos uva‐ursi), garden pea (Pisum sativum), Norway spruce (Picea abies) and large‐leaved lime (Tilia platyphyllos) extracts were the most active. Additionally, the activity of selected extracts with removed polyphenols was measured. Extracts of bearberry, garden
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In this study we have investigated the surface rheological properties of oil-water interfaces stabilized by fibrils from lysozyme (long and semi-flexible and short and rigid ones), fibrils from ovalbumin (short and semi-flexible), lysozyme-pectin complexes, or ovalbumin-pectin complexes. We have compared these properties with those of interfaces stabilized by the native proteins. The surface dilatational and surface shear moduli were determined using an automated drop tensiometer and a stress controlled rheometer with biconical disk geometry. Results show that interfaces stabilized by complexes of these proteins with high-methoxyl pectin have higher surface shear and dilatational moduli than interfaces stabilized by the native proteins only. The interfaces stabilized by ovalbumin and lysozyme complexes have comparable shear and dilatational moduli though ovalbumin-pectin complexes are twice as large in radius as lysozyme-pectin complexes. Under most of the experimental conditions, interfaces ...
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Assay Lipase activity in 1 hr in cell culture media, cell/tissue extracts, and biofluids with Lipase Activity Assay Kit III (Fluorometric) ab118969. For microplate readers.
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Colipases at the US National Library of Medicine Medical Subject Headings (MeSH) PDBe-KB provides an overview of all the ...
"Colipases" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... This graph shows the total number of publications written about "Colipases" by people in this website by year, and whether " ... Below are the most recent publications written about "Colipases" by people in Profiles. ...
Colipases / metabolism * Dose-Response Relationship, Drug * Enzyme Activation / drug effects * Fatty Acids, Unsaturated / ...
... bird or marine colipases. CoSPL was found to be less effective activator of bird and mammal pancreatic lipases than for the ...
Pancreatic lipases and their complexes with colipases and inhibitors: crystallization and crystal packing. (1997) ... Pancreatic lipases and their complexes with colipases and inhibitors: Crystallization and crystal packing (1998) ...
Pancreatic lipases and their complexes with colipases and inhibitors: crystallization and crystal packing. (1997) ... Pancreatic lipases and their complexes with colipases and inhibitors: Crystallization and crystal packing (1998) ...
Colipases at the US National Library of Medicine Medical Subject Headings (MeSH) PDBe-KB provides an overview of all the ...
Define Coliseum (theatre). Coliseum (theatre) synonyms, Coliseum (theatre) pronunciation, Coliseum (theatre) translation, English dictionary definition of Coliseum (theatre). n. Variant of coliseum. n a variant spelling of coliseum n an amphitheatre in Rome built about 75-80 ad n. 1. an ancient amphitheater in Rome, begun A.D....
Define colinear. colinear synonyms, colinear pronunciation, colinear translation, English dictionary definition of colinear. adj. 1. Collinear. 2. Genetics a. Characterized by correspondence between the linear sequence of nucleotides in a gene and the linear sequence of amino...
Colipases. 2. Cognition Disorders. 2. Clozapine. 2. Circadian Rhythm. 2. Child Development. ...
Fingerprint Dive into the research topics of Adsorption of bile salts and pancreatic colipase and lipase onto digalactosyldiacylglycerol and dipalmitoylphosphatidylcholine monolayers. Together they form a unique fingerprint. ...
Colipases. 2. Cognition Disorders. 2. Clozapine. 2. Circadian Rhythm. 2. Child Development. ...
Colipases (0) * Contractile Proteins (1) * Cystatins (0) * Cystine-Knot Miniproteins (0) * Cytoskeletal Proteins (0) ...
Colipases (0) * Contractile Proteins (1) * Cystatins (0) * Cystine-Knot Miniproteins (0) * Cytoskeletal Proteins (0) ...
TY - JOUR. T1 - The Arg92Cys colipase polymorphism impairs function and secretion by increasing protein misfolding. AU - Xiao, Xunjun. AU - Ferguson, Michael R.. AU - Magee, Kelsey E.. AU - Hale, Pamela D.. AU - Wang, Yan. AU - Lowe, Mark E.. PY - 2013/2/1. Y1 - 2013/2/1. N2 - Colipase is essential for efficient fat digestion. An arginine-to-cysteine polymorphism at position 92 of colipase (Arg92Cys) associates with an increased risk for developing type-2 diabetes through an undefined mechanism. To test our hypothesis that the extra cysteine increases colipase misfolding, thereby altering its intracellular trafficking and function, we expressed Cys92 colipase in HEK293T cells. Less Cys92 colipase is secreted and more is retained intracellularly in an insoluble form compared with Arg92 colipase. Nonreducing gel electrophoresis suggests the folding of secreted Cys92 colipase differs from Arg92 colipase. Cys92 colipase misfolding does not trigger the unfolded protein response (UPR) or endoplasmic ...
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... bird or marine colipases. CoSPL was found to be less effective activator of bird and mammal pancreatic lipases than for the ... the activation of SPL by colipases from other species and the capacity of CoSPL to activate various pancreatic lipases. (A) ... Table 3 Kinetic parameters between stingray lipase and colipases of various species deduced from figure 4A. Full size table ... Only bird colipases reacted strongly with anti CoOPL (Figure. 1B). No cross-immunoreactivity was detected between anti-CoOPL ...
Q: How do you activate colipases?. A: by chymotrypsin, which cleaves the pre-colipase. ...
This graph shows the total number of publications written about "Receptors, Drug" by people in Harvard Catalyst Profiles by year, and whether "Receptors, Drug" was a major or minor topic of these publication ...
Wong HY, Wang GM, Croessmann S, Zabransky DJ, Chu D, Garay JP, Cidado J, Cochran RL, Beaver JA, Aggarwal A, Liu ML, Argani P, Meeker A, Hurley PJ, Lauring J, Park BH. TMSB4Y is a candidate tumor suppressor on the Y chromosome and is deleted in male breast cancer. Oncotarget. 2015 Dec 29; 6(42):44927-40 ...
Colipases Research. Contractile Proteins Research. Cystatins Research. Cytoskeletal Proteins Research. Dental Enamel Proteins ...
Looking for online definition of colinearity in the Medical Dictionary? colinearity explanation free. What is colinearity? Meaning of colinearity medical term. What does colinearity mean?
Light absorbing proteins and protein prosthetic groups found in certain microorganisms. Some microbial photoreceptors initiate specific chemical reactions which signal a change in the environment, while others generate energy by pumping specific ions across a cellular membrane ...
Noh HJ, Tang R, Flannick J, ODushlaine C, Swofford R, Howrigan D, Genereux DP, Johnson J, van Grootheest G, Grünblatt E, Andersson E, Djurfeldt DR, Patel PD, Koltookian M, M Hultman C, Pato MT, Pato CN, Rasmussen SA, Jenike MA, Hanna GL, Stewart SE, Knowles JA, Ruhrmann S, Grabe HJ, Wagner M, Rück C, Mathews CA, Walitza S, Cath DC, Feng G, Karlsson EK, Lindblad-Toh K. Integrating evolutionary and regulatory information with a multispecies approach implicates genes and pathways in obsessive-compulsive disorder. Nat Commun. 2017 10 17; 8(1):774 ...
... pancreatic lipases and co-lipases, which facilitate a special environment in the gut that activates and promotes better ...
The histidine-tagged lipases from the culture supernatant were purified using Ni-NTA chromatography by ÄKTA purifier (GE Co.). Lipases ...
Amino Acid Sequence, Amino Acids, metabolism, Animals, Base Sequence, Blotting, Northern, Cloning, Molecular, Colipases, ...
Proteins that share the common characteristic of binding to carbohydrates. Some ANTIBODIES and carbohydrate-metabolizing proteins (ENZYMES) also bind to carbohydrates, however they are not considered lectins. PLANT LECTINS are carbohydrate-binding proteins that have been primarily identified by their hemagglutinating activity (HEMAGGLUTININS). However, a variety of lectins occur in animal species where they serve diverse array of functions through specific carbohydrate recognition ...
All Proteins are long linear, unbranched polypeptides and as caloric nutrients built, usually built from strings of about 20 different alpha-amino acid
This graph shows the total number of publications written about "Mitochondrial Proteins" by people in this website by year, and whether "Mitochondrial Proteins" was a major or minor topic of these publications ...
  • Colipases" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (ucdenver.edu)