Colipases: Colipase I and II, consisting of 94-95 and 84-85 amino acid residues, respectively, have been isolated from porcine pancreas. Their role is to prevent the inhibitory effect of bile salts on the lipase-catalyzed intraduodenal hydrolysis of dietary long-chain triglycerides.Succinylcholine: A quaternary skeletal muscle relaxant usually used in the form of its bromide, chloride, or iodide. It is a depolarizing relaxant, acting in about 30 seconds and with a duration of effect averaging three to five minutes. Succinylcholine is used in surgical, anesthetic, and other procedures in which a brief period of muscle relaxation is called for.Intestine, Small: The portion of the GASTROINTESTINAL TRACT between the PYLORUS of the STOMACH and the ILEOCECAL VALVE of the LARGE INTESTINE. It is divisible into three portions: the DUODENUM, the JEJUNUM, and the ILEUM.Lipase: An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.Bacteriocins: Substances elaborated by specific strains of bacteria that are lethal against other strains of the same or related species. They are protein or lipopolysaccharide-protein complexes used in taxonomy studies of bacteria.Hydrogen-Ion Concentration: The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)Stomach: An organ of digestion situated in the left upper quadrant of the abdomen between the termination of the ESOPHAGUS and the beginning of the DUODENUM.Bile Acids and Salts: Steroid acids and salts. The primary bile acids are derived from cholesterol in the liver and usually conjugated with glycine or taurine. The secondary bile acids are further modified by bacteria in the intestine. They play an important role in the digestion and absorption of fat. They have also been used pharmacologically, especially in the treatment of gallstones.Peptides: Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.Signal Transduction: The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Peptide Fragments: Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.Transcription Factors: Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.Psychology, Experimental: The branch of psychology which seeks to learn more about the fundamental causes of behavior by studying various psychologic phenomena in controlled experimental situations.Neoplasm Proteins: Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (BIOMARKERS, TUMOR) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm.Paraganglioma: A neural crest tumor usually derived from the chromoreceptor tissue of a paraganglion, such as the carotid body, or medulla of the adrenal gland (usually called a chromaffinoma or pheochromocytoma). It is more common in women than in men. (Stedman, 25th ed; from Segen, Dictionary of Modern Medicine, 1992)Tumor Markers, Biological: Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.Tumor Suppressor Protein p53: Nuclear phosphoprotein encoded by the p53 gene (GENES, P53) whose normal function is to control CELL PROLIFERATION and APOPTOSIS. A mutant or absent p53 protein has been found in LEUKEMIA; OSTEOSARCOMA; LUNG CANCER; and COLORECTAL CANCER.Body Fluids: Liquid components of living organisms.Genes, Tumor Suppressor: Genes that inhibit expression of the tumorigenic phenotype. They are normally involved in holding cellular growth in check. When tumor suppressor genes are inactivated or lost, a barrier to normal proliferation is removed and unregulated growth is possible.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Amyloid: A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.Plaque, Amyloid: Accumulations of extracellularly deposited AMYLOID FIBRILS within tissues.Amyloid beta-Peptides: Peptides generated from AMYLOID BETA-PEPTIDES PRECURSOR. An amyloid fibrillar form of these peptides is the major component of amyloid plaques found in individuals with Alzheimer's disease and in aged individuals with trisomy 21 (DOWN SYNDROME). The peptide is found predominantly in the nervous system, but there have been reports of its presence in non-neural tissue.Silk: A continuous protein fiber consisting primarily of FIBROINS. It is synthesized by a variety of INSECTS and ARACHNIDS.Fibroins: Fibrous proteins secreted by INSECTS and SPIDERS. Generally, the term refers to silkworm fibroin secreted by the silk gland cells of SILKWORMS, Bombyx mori. Spider fibroins are called spidroins or dragline silk fibroins.Alzheimer Disease: A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)Serum Amyloid A Protein: An ACUTE PHASE REACTION protein present in low concentrations in normal sera, but found at higher concentrations in sera of older persons and in patients with AMYLOIDOSIS. It is the circulating precusor of amyloid A protein, which is found deposited in AA type AMYLOID FIBRILS.Rhizopus: A genus of zygomycetous fungi of the family Mucoraceae, order MUCORALES, a common saprophyte and facultative parasite of mature fruits and vegetables. It may cause cerebral mycoses in diabetes and cutaneous infection in severely burned patients.Glycosylation: The chemical or biochemical addition of carbohydrate or glycosyl groups to other chemicals, especially peptides or proteins. Glycosyl transferases are used in this biochemical reaction.Pichia: Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.PolysaccharidesAsparagine: A non-essential amino acid that is involved in the metabolic control of cell functions in nerve and brain tissue. It is biosynthesized from ASPARTIC ACID and AMMONIA by asparagine synthetase. (From Concise Encyclopedia Biochemistry and Molecular Biology, 3rd ed)Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).Protein Folding: Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.Science: The study of natural phenomena by observation, measurement, and experimentation.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Protein Interaction Mapping: Methods for determining interaction between PROTEINS.

Hydrolysis of emulsified mixtures of triacylglycerols by pancreatic lipase. (1/105)

Hydrolysis of the emulsified mixture of short-chain triacylglycerols by porcine pancreatic lipase in the presence of procolipase and micellar sodium taurodeoxycholate has been studied. Increase in the content of tributyrin and trioctanoin in the mixture with triacetin had highly cooperative effects on the formation of the interfacial lipase procolipase complex. Abrupt enhancement of the complex stability was observed in the presence of 0.4-0.6 mol mol-1 of tributyrin or 0.58 mol mol-1 of trioctanoin in the substrate phase. The affinity of lipase towards interfacially bound procolipase for the trioctanoin containing 0.07-0.42 mol mol-1 of triacetin was approximately three times higher than that for pure trioctanoin. The cooperative processes involved in complex formation did not contribute to the affinity of the interfacial lipase/(pro)colipase complex towards substrate molecules and its catalytic activity.  (+info)

Pancreatic lipase/colipase-mediated triacylglycerol hydrolysis is required for cholesterol transport from lipid emulsions to intestinal cells. (2/105)

This study tested the hypothesis that dietary cholesterol uptake by intestinal cells is dependent on the structure and composition of the lipid carriers in the extracellular milieu. In in vivo experiments with female C57BL/6 mice, cholesterol absorption from phospholipid/triacylglycerol emulsions was significantly reduced by administration of tetrahydrolipstatin, an inhibitor of pancreatic lipase. This inhibitor had no effect on the absorption of cholesterol from phospholipid vesicles. The importance of pancreatic-lipase-mediated triacylglycerol hydrolysis for cholesterol transport from emulsions to intestinal cells was confirmed by in vitro experiments with rat IEC-6 intestinal cells. Cellular uptake of cholesterol from emulsions with a phospholipid/triacylglycerol molar ratio of <0.3 could be stimulated by pancreatic lipase/colipase hydrolysis of the core neutral lipids. However, pancreatic lipase/colipase was ineffective in hydrolysing triacylglycerols in emulsions with a phospholipid/triacylglycerol molar ratio of >0.3. Phospholipase A2-mediated hydrolysis of the surface phospholipids was necessary prior to triacylglycerol hydrolysis in these phospholipid-rich emulsions and to the stimulation of cholesterol transport from these particles to IEC-6 cells. The data also revealed that minimal triacylglycerol hydrolysis was sufficient to significantly increase cholesterol transport from lipid emulsions to the intestinal cells. Thus the products of triacylglycerol hydrolysis, namely monoacylglycerol and non-esterified fatty acids, are key determinants in mediating cholesterol transport from lipid emulsions to intestinal cells. Taken together, these results support the hypothesis that remodelling of the surface and core components of lipid carriers is necessary prior to absorption of dietary cholesterol from the gastrointestinal tract.  (+info)

Colipase: structure and interaction with pancreatic lipase. (3/105)

Colipase is a small protein cofactor needed by pancreatic lipase for the efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising an active conformation and considerably increasing the overall hydrophobic binding site. Structural studies of the complex and of colipase alone have clearly revealed the functionality of its architecture. Interestingly, a structural analogy has recently been discovered between colipase and a domain in a developmental protein (Dickkopf), based on sequence analogy and homology modeling. Whether this structural analogy implies a common function (lipid interaction) remains to be clarified. Structural analogies have also been recognised between the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. These non-catalytic domains in the latter enzymes are important for interaction with membranes. It has not been established if these domains are also involved in eventual protein cofactor binding as is the case for pancreatic lipase.  (+info)

Critical role of micelles in pancreatic lipase activation revealed by small angle neutron scattering. (4/105)

In the duodenum, pancreatic lipase (PL) develops its activity on triglycerides by binding to the bile-emulsified oil droplets in the presence of its protein cofactor pancreatic colipase (PC). The neutron crystal structure of a PC-PL-micelle complex (Hermoso, J., Pignol, D., Penel, S., Roth, M., Chapus, C., and Fontecilla-Camps, J. C. (1997) EMBO J. 16, 5531-5536) has suggested that the stabilization of the enzyme in its active conformation and its adsorption to the emulsified oil droplets are mediated by a preformed lipase-colipase-micelle complex. Here, we correlate the ability of different amphypathic compounds to activate PL, with their association with PC-PL in solution. The method of small angle neutron scattering with D(2)O/H(2)O contrast variation was used to characterize a solution containing PC-PL complex and taurodeoxycholate micelles. The resulting radius of gyration (56 A) and the match point of the solution indicate the formation of a ternary complex that is similar to the one observed in the neutron crystal structure. In addition, we show that either bile salts, lysophospholipids, or nonionic detergents that form micelles with radii of gyration ranging from 13 to 26 A are able to bind to the PC-PL complex, whereas smaller micelles or nonmicellar compounds are not. This further supports the notion of a micelle size-dependent affinity process for lipase activation in vivo.  (+info)

Effect of high-fat diet, surrounding temperature, and enterostatin on uncoupling protein gene expression. (5/105)

Nonshivering thermogenesis induced in brown adipose tissue (BAT) during high-fat feeding is mediated through uncoupling protein 1 (UCP1). UCP2 is a recently identified homologue found in many tissues. To determine the role of UCP1 and UCP2 in thermoregulation and energy balance, we investigated the long-term effect of high-fat feeding on mRNA levels in mice at two different ambient temperatures. We also treated mice with the anorectic peptide enterostatin and compared mRNA levels in BAT, white adipose tissue (WAT), stomach, and duodenum. Here, we report that high-fat feeding at 23 degrees C increased UCP1 and UCP2 levels in BAT four- and threefold, respectively, and increased UCP2 levels fourfold in WAT. However, at 29 degrees C, UCP1 decreased, whereas UCP2 remained unchanged in BAT and increased twofold in WAT. Enterostatin increased UCP1 and decreased UCP2 mRNA in BAT. In stomach and duodenum, high-fat feeding decreased UCP2 mRNA, whereas enterostatin increased it. Our results suggest that the regulation of uncoupling protein mRNA levels by high-fat feeding is dependent on ambient temperature and that enterostatin is able to modulate it.  (+info)

Fats infused intraduodenally affect the postprandial secretion of the exocrine pancreas and the plasma concentration of cholecystokinin but not of peptide YY in growing pigs. (6/105)

In pigs, the spontaneous secretion of the exocrine pancreas and the release of cholecystokinin (CCK) and peptide YY (PYY) after intraduodenal infusion of fully saturated synthetic fats differing in chain length was studied. Growing pigs (n = 6) were prepared with pancreatic duct catheters, duodenal T-cannulas and catheters placed in the jugular vein. The pigs were fed 2 g/100 g body twice daily. Beginning with the morning feeding, a medium-chain triglyceride (MCT: glycerol tricaprylate), a long-chain triglyceride (LCT: glycerol tristearate) or saline was infused at a rate of 0.1 g/100 g body. Pancreatic juice was collected, beginning 1 h preprandially until 3 h postprandially. Blood samples were obtained 15 min preprandially and 15, 45, 90 and 150 min postprandially. The infusion of MCT evoked a change in the trend of the curve for the volume of secretion of pancreatic juice, lipase and colipase concentrations and outputs. The trend of the curve did not change over time for CCK and PYY. Differences between the trends of the curves for the saline and MCT treatment were observed for volume of secretion, protein output, lipase content and output, trypsin and colipase output. Differences in the trends of the curves between MCT and LCT were obtained for the outputs of protein, lipase and colipase. Plasma CCK levels were lower as a result of the MCT treatment compared with the saline and LCT treatments. The results suggest an immediate, distinguished response of the porcine exocrine pancreas to fats differing in chain length.  (+info)

Interactions of bile salt micelles and colipase studied through intermolecular nOes. (7/105)

Colipase is a small protein (10 kDa), which acts as a protein cofactor for the pancreatic lipase. Various models of the activated ternary complex (lipase-colipase-bile salt micelles) have been proposed using detergent micelles, but no structural information has been established with bile salt micelles. We have investigated the organization of sodium taurodeoxycholate (NaTDC) micelles and their interactions with pig and horse colipases by homonuclear nuclear magnetic resonance (NMR) spectroscopy. The NMR data supply evidence that the folding of horse colipase is similar to that already described for pig colipase. Intermolecular nuclear Overhauser effects have shown that two conserved aromatic residues interact with NaTDC micelles.  (+info)

The lipase C-terminal domain. A novel unusual inhibitor of pancreatic lipase activity. (8/105)

In vertebrates, dietary fat digestion mainly results from the combined effect of pancreatic lipase, colipase, and bile. It has been proposed that in vivo lipase adsorption on oil-water emulsion is mediated by a preformed lipase-colipase-mixed micelle complex. The main lipase-colipase binding site is located on the C-terminal domain of the enzyme. We report here that in vitro the isolated C-terminal domain behaves as a potent noncovalent inhibitor of lipase and that the inhibitory effect is triggered by the presence of micelles. Lipase inhibition results from the formation of a nonproductive C-terminal domain-colipase-micelle ternary complex, which competes for colipase with the active lipase-colipase-micelle ternary complex, thus diverting colipase from its lipase-anchoring function. The formation of such a complex has been evidenced by molecular sieving experiments. This nonproductive complex lowers the amount of active lipase thus reducing lipolysis. Preliminary experiments performed in rats show that the C-terminal domain also behaves as an inhibitor in vivo and thus could be considered a potential new tool for specifically reducing intestinal lipolysis.  (+info)

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pancreatic lipase related protein 2: from human (hPLRP2) & guinea pig (gPLRP2); highly homologous to human pancreatic lipase (hPL), but its lipolytic activity is profoundly different from hPL; amino acid sequence has been determined
Although the structure of Human Pancreatic Lipase has been documented through the X-ray crystallography, the knowledge about the molecular rearrangement and dynamic equilibrium in the structure (particularly in the catalytic triad and lid domains) is very scanty. The structural fluctuations and conformational changes undergo by Human Pancreatic Lipase (HPL) with and without colipase were computationally investigated through molecular dynamics simulation technique using GROMACS 2018.4, MOE 2016.0801 and VMD softwares in order to gain insight into the complex transitions at different domains. The structural stability was revealed vis-a-vis Root Mean Square Deviation (RMSD) and Root Mean Square Fluctuations (RMSF) plots. The levels of compactness/folding and conformational changes of the protein were determined using Radius of gyration and secondary analysis respectively. Salt bridge analysis gives more ionic pairs interactions than experimentally determined results. Results show that though both proteins
Visceral adipose tissue plays a critical role in numerous diseases. While imaging studies often show adipose involvement in abdominal diseases, their outcomes may vary from being a mild self limited illness to one with systemic inflammation and organ failure. We therefore compared the pattern of visceral adipose injury during acute pancreatitis and acute diverticulitis to determine its role in organ failure. Acute pancreatitis-associated adipose tissue had ongoing lipolysis in the absence of adipocyte triglyceride lipase (ATGL). Pancreatic lipase injection into mouse visceral adipose tissue hydrolyzed adipose triglyceride and generated excess non-esterified fatty acids (NEFA), which caused organ failure in the absence of acute pancreatitis. Pancreatic triglyceride lipase (PNLIP) increased in adipose tissue during pancreatitis and entered adipocytes by multiple mechanisms, hydrolyzing adipose triglyceride and generating excessive NEFA. During pancreatitis, obese PNLIP knockout mice, unlike obese ...
Build: Wed Jun 21 18:33:50 EDT 2017 (commit: 4a3b2dc). National Center for Advancing Translational Sciences (NCATS), 6701 Democracy Boulevard, Bethesda MD 20892-4874 • 301-435-0888. ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
SWISS-MODEL Template Library (SMTL) entry for 1pcn.1. SOLUTION STRUCTURE OF PORCINE PANCREATIC PROCOLIPASE AS DETERMINED FROM 1H HOMONUCLEAR TWO-AND THREE-DIMENSIONAL NMR
We present the behaviour of particles with chemical and geometrical anisotropy at a planar oil-water interface. We find that Janus cylinders with a small aspect ratio adopt an upright configuration, whereas the particles with a large aspect ratio exhibit both the upright and tilted configurations, which can
This entry represents a domain found in a variety of membrane or lipid associated proteins. It is known as the PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology) domain, is found in a variety of membrane or lipid associated proteins. Structurally, this domain forms a beta-sandwich composed of two sheets of four strands each [(PUBMED:10469604), (PUBMED:11985859), (PUBMED:11412104)]. The most highly conserved regions coincide with the beta-strands, with most of the highly conserved residues being buried within the protein. An exception to this is a surface lysine or arginine that occurs on the surface of the fifth beta-strand of the eukaryotic domains. In pancreatic lipase, the lysine in this position forms a salt bridge with the procolipase protein. The conservation of a charged surface residue may indicate the location of a conserved ligand-binding site. It is thought that this domain may mediate membrane attachment via other protein binding partners.. ...
This entry represents a domain found in a variety of membrane or lipid associated proteins. It is known as the PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology) domain, is found in a variety of membrane or lipid associated proteins. Structurally, this domain forms a beta-sandwich composed of two sheets of four strands each [(PUBMED:10469604), (PUBMED:11985859), (PUBMED:11412104)]. The most highly conserved regions coincide with the beta-strands, with most of the highly conserved residues being buried within the protein. An exception to this is a surface lysine or arginine that occurs on the surface of the fifth beta-strand of the eukaryotic domains. In pancreatic lipase, the lysine in this position forms a salt bridge with the procolipase protein. The conservation of a charged surface residue may indicate the location of a conserved ligand-binding site. It is thought that this domain may mediate membrane attachment via other protein binding partners.. ...
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In molecular biology the PLAT domain is a protein domain that is found in a variety of membrane or lipid associated proteins. It is called the PLAT (Polycystin-1, Lipoxygenase, Alpha-Toxin) domain or LH2 (Lipoxygenase homology) domain. The known structure of pancreatic lipase shows this domain binds to procolipase Pfam PF01114, which mediates membrane association. This domain is found in a variety of membrane or lipid associated proteins. It forms a beta-sandwich composed of two β-sheets of four β-strands each. ALOX12; ALOX12B; ALOX12P2; ALOX15; ALOX15B; ALOX5; ALOXE3; LIPC; LIPG; LOXHD1; LPL; PKD1; PKD1L1; PKD1L2; PKD1L3; PKDREJ; PNLIP; PNLIPRP1; PNLIPRP2; PNLIPRP3; RAB6IP1; van Tilbeurgh H, Egloff MP, Martinez C, Rugani N, Verger R, Cambillau C (April 1993). "Interfacial activation of the lipase-procolipase complex by mixed micelles revealed by X-ray crystallography". Nature. 362 (6423): 814-20. doi:10.1038/362814a0. PMID 8479519. Bateman A, Sandford R (1999). "The PLAT domain: a new piece ...
Our long-term goal is to elucidate the molecular mechanisms of dietary fat digestion by pancreatic lipases. In this application, we will focus on the steps in l...
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Synonyms: related, related to Definition: being connected either logically or causally or by shared characteristics Usage: painting and the related arts; school-related activities; related to micelle formation is the...ability of detergent actives to congregate at oil-water interfaces Hypernyms: connected, affiliated, attached Definition: being joined in close association Usage: affiliated clubs; all art schools whether independent or attached to universities Hypernyms: age-related Definition: changing (increasing or decreasing) as an individuals age increases Hypernyms: bound up Definition: closely or inseparably connected or associated with Usage: his career is bound up with the fortunes of the enterprise Hypernyms: cognate Definition: having the same ancestral language Usage: cognate languages Hypernyms: cognate, connate Definition: related in nature Usage: connate qualities Hypernyms: coreferent Definition: related by sharing a symbolic link to a concrete object or an abstraction Usage: two ...
TY - JOUR. T1 - Behavior of plant-dairy protein blends at air-water and oil-water interfaces. AU - Hinderink, E.B.A.. AU - Sagis, L.M.C.. AU - Schroen, C.G.P.H.. AU - Berton-Carabin, C.C.. PY - 2020/8. Y1 - 2020/8. N2 - Recent work suggests that using blends of dairy and plant proteins could be a promising way to mitigate sustainability and functionality concerns. Many proteins form viscoelastic layers at fluid interfaces and provide physical stabilization to emulsion droplets; yet, the interfacial behavior of animal-plant protein blends is greatly underexplored. In the present work, we considered pea protein isolate (PPI) as a model legume protein, which was blended with well-studied dairy proteins (whey protein isolate (WPI) or sodium caseinate (SC)). We performed dilatational rheology at the air-water and oil-water interface using an automated drop tensiometer to chart the behavior and structure of the interfacial films, and to highlight differences between films made with either blends, or ...
ALARCÓN, C. & CUBILLOS, L.A. & ACUÑA, E. (2011) Length-based growth, maturity and natural mortality of the cockfish Callorhinchus callorhynchus (Linnaeus, 1758) off Coquimbo, Chile. Environmental Biology of Fishes, 92 (1): 65-78 http://dx.doi.org/10.1007/s10641-011-9816-0. BABU, C. & RAMACHANDRAN, S. & VARGHESE, B.C. (2011) New record of sixgill sting ray Hexatrygon bickelli Heemstra and Smith, 1980 from south-west coast of India. Indian Journal of Fisheries, 58 (2): 137-139. BACHA, A.B. & KARRAY, A. & DAOUD, L. & BOUCHAALA, E. & ALI, M.B. & GARGOURI, Y. & ALI, Y.B. (2011) Biochemical properties of pancreatic colipase from the common stingray Dasyatis pastinaca. Lipids in Health and Disease, 10: 69, 1-7 http://dx.doi.org/10.1186/1476-511X-10-69. BLANCO-PARRA, M.P. & NIÑO-TORRES, C.A. (2011) Morphological abnormality in a diamond stingray, Dasyatis dipterura (Jordan & Gilbert, 1880) (Chondrichthyes: Dasyatidae), from the Gulf of California, Mexico Cahiers de Biologie Marine, 52 (3): ...
1ETH: Lipase activation by nonionic detergents. The crystal structure of the porcine lipase-colipase-tetraethylene glycol monooctyl ether complex.
Coypu (Myocastor coypus) at Galikos river. I have visited Galikos and Kalochori lagoon many times and Ive always wanted to see a coypu. Out of sheer luck the last I were there I saw one sitting atop some reeds ...
Find api oil-water separator articles on Environmental XPRT, the worlds largest environmental industry marketplace and information resource.
Lipids, or fats, are digested and absorbed in the small intestine. In this lesson, you will learn how bile salts emulsify fat so pancreatic lipase...
Garden of Life Order Line - 888.244.8948 Omega-zyme aids in the digestion of proteins, carbohydrates, fats, dairy and other difficult to digest foods. Omegazyme contains up to 20 times more lipase for fat digestion than the leading brands.
TY - JOUR. T1 - Effect of gastric lipase on turbidimetric and dry-film methods for measuring pancreatic lipase [1]. AU - Kazmierczak, S. C.. AU - Van Lente, F.. AU - Tetrault, G. A.. PY - 1992/12/1. Y1 - 1992/12/1. UR - http://www.scopus.com/inward/record.url?scp=0027098002&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0027098002&partnerID=8YFLogxK. M3 - Letter. C2 - 1458599. AN - SCOPUS:0027098002. VL - 38. SP - 2555. EP - 2556. JO - Clinical Chemistry. JF - Clinical Chemistry. SN - 0009-9147. IS - 12. ER - ...
LIGAND BASED PHARMACOPHORE MODELING, VIRTUAL SCREENING AND MOLECULAR DOCKING STUDIES TO DESIGN NOVEL PANCREATIC LIPASE INHIBITORS
Obesity is a serious public health concern with an increasing prevalence worldwide. The diet contributes strongly to this problem and high-fat food leads to unhealthy conditions. Fat digestion is an energy intensive process as it requires hydrolysis to allow the body to profit from this nutrient. This additional energy expenditure is also present in a highly redundant hormonal regulation system for fat storage which is converted in not a readily accessible form (therefore, an upstream is required in order to tackle fat-related diseases). Due to the low water solubility of oils and fats, the lipase catalyzed reactions take place at a specific locus, the oil-water interface. Despite considerable efforts in the past the effects of the interfacial composition on lipase activity have been only qualitatively understood until recently. It has recently been undertaken a detailed study of the interfacial behavior of lipases, their substrates and products which have contributed to shed light into the mechanism of
Talaromyces thermophilus lipase (TTL) was found to hydrolyze monogalactosyl diacylglycerol (MGDG) and digalactosyl diacylglycerol (DGDG) substrates presented in various forms to the enzyme. Different assay techniques were used for each substrate: pHstat with dioctanoyl galactolipid-bile salt mixed micelles, barostat with dilauroyl galactolipid monomolecular films spread at the air-water interface, and UV absorption using a novel MGDG substrate containing α-eleostearic acid as chromophore and coated on microtiter plates. The kinetic properties of TTL were compared to those of the homologous lipase from Thermomyces lanuginosus (TLL), guinea pig pancreatic lipase-related protein 2 and Fusarium solani cutinase. TTL was found to be the most active galactolipase, with a higher activity on micelles than on monomolecular films or surface-coated MGDG. Nevertheless, the UV absorption assay with coated MGDG was highly sensitive and allowed measuring significant activities with about 10 ng of enzymes, against 100
Bile salt-dependent lipase (or BSDL), also known as carboxyl ester lipase (or CEL) is an enzyme produced by the adult pancreas and aids in the digestion of fats. Bile salt-stimulated lipase (or BSSL) is an equivalent enzyme found within breast milk. BSDL has been found in the pancreatic secretions of all species in which it has been looked for. BSSL, originally discovered in the milk of humans and various other primates, has since been found in the milk of many animals including dogs, cats, rats, and rabbits. More than 95% of the fat present in human milk and in infant formulas is in the form of triacylglycerols (TG). In adults, TGs are thought to be broken down or hydrolyzed mainly by the colipase-dependent lipase enzyme. In the newborn, CDL activity in the duodenum is lower than in adults. Both BSDL and BSSL have a broad substrate specificity and, like CDL, are capable of hydrolyzing triacylglycerides (in addition to phospholipids, esters of cholesterol, and lipid-soluble vitamins). In ...
Semantic Scholar extracted view of [Effect of pancreatic lipase on the development of experimental atherosclerosis]. by I. P. Bondarenko
INTRODUCTION. Lipases (triacylglycerol acylhydrolases, EC3.1.1.3) are water-soluble enzymes that catalyze the hydrolysis of ester bonds of insoluble triacylglycerols, releasing free fatty acids, mono-or diacylglycerolin into the oil-water interface (Treichel et al., 2010). These enzymes catalyze a wide range of reactions such as hydrolysis, esterification, trans-esterification, alcoholysis, acidolysis and aminolysis (Joseph; Ramteke; Thomas, 2008), and may be of animal, microbial or plant origin, with variations in catalytic properties, and can be obtained by solid-state fermentation or submerged fermentation (Annibale et al., 2006; Rigo et al., 2010; Papagora; Roukas; Kotzekidou, 2013).. Lipases are an important group of enzymes with various applications in the food, chemical and pharmaceutical industry, besides having great interest for the treatment of effluents with high lipid content (Jeganathan; Nakhla; Bassi, 2007; Ertugrul; Donnez; Takac, 2007). The use of lipases in effluents with high ...
GASTROENTEROPATHY CHLORAMPHENICOL PROTOZOACIDES ENTEROPATHIC ENTERORRHAGIA ENTEROSALYL ENTEROSARINE ENTEROSEPTOL ENTEROSGEL ENTEROSIN ENTEROSOLUBILITY Procesals ENTEROSTATIN ENTEROTOXEMIA ENTEROTOXEMIA-VACCINE ENTEROTOXIGENIC ENTEROTOXIN ENTEROTOXIN-A-STAPH ENTEROTOXIN-B-HEAT-LABILE ENTEROTOXIN-HEAT-LABILE-E. 4 was higher than that at pH 5.
Pancreatic lipase. Hydrolyzes triacylglycerols (fats) into fatty acids and glycerol. Contains 19.6 USP units per milligram. Keep refrigerated.CAS: 9001-62-1Health Risk: 0Flammability: 0Reactivity: 1Synonym: Steapsin
The enzymes are not active when they are first made within the pancreas (otherwise they would digest the pancreas as well) but when they pass into the bowel they are activated by the juices in the bowel. The main enzymes are called amylase (which digests carbohydrates), trypsin (which digests protein) and lipase (which digests fats). The bile, which comes from the liver, is also very important for the digestion of fat because it acts like a soap and breaks up the fat into minute droplets so that the pancreatic lipase can digest it ...
The enzymes are not active when they are first made within the pancreas (otherwise they would digest the pancreas as well) but when they pass into the bowel they are activated by the juices in the bowel. The main enzymes are called amylase (which digests carbohydrates), trypsin (which digests protein) and lipase (which digests fats). The bile, which comes from the liver, is also very important for the digestion of fat because it acts like a soap and breaks up the fat into minute droplets so that the pancreatic lipase can digest it ...
Optimizes Fat Digestion. Fats are not normally digested until they reach the small intestine, causing the body to use energy inefficiently. Essential fats promote well-being, but some individuals do not metabolize them well.
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Assay Lipase activity in 1 hr in cell culture media, cell/tissue extracts, and biofluids with Lipase Activity Assay Kit III (Fluorometric) ab118969. For microplate readers.
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TY - JOUR. T1 - Effect of germinated brown rice extracts on pancreatic lipase, adipogenesis and lipolysis in 3T3-L1 adipocytes. AU - See Meng, Lim. AU - Goh, Yong Meng. AU - Kuan, Wen Bin. AU - Loh, Su Peng. PY - 2014/11/3. Y1 - 2014/11/3. N2 - Background: This study investigated anti-obesity effects of seven different solvent (n-hexane, toluene, dicholoromethane, ethyl acetate, absolute methanol, 80% methanol and deionized water) extracts of germinated brown rice (GBR) on pancreatic lipase activity, adipogenesis and lipolysis in 3T3-L1 adipocytes. Methods: GBR were extracted separately by employing different solvents with ultrasound-assisted. Pancreatic lipase activity was determined spectrophotometrically by measuring the hydrolysis of p-nitrophenyl butyrate (p-NPB) to p-nitrophenol at 405 nm. Adipogenesis and lipolysis were assayed in fully differentiated 3T3-L1 adipocytes by using Oil Red O staining and glycerol release measurement. Results: GBR extract using hexane showed the highest ...
Background about pancreatic lipase... The prevalence of obesity is increasing at an alarming rate, but, unfortunately, only a few medications are currently on the market. Obesity is primarily regarded as a disorder of lipid metabolism and the enzymes involved in this process could be selectively targeted to develop antiobesity drugs. Recently, newer approaches for the treatment of obesity have involved inhibition of dietary triglyceride absorption via inhibition of pancreatic lipase (PL) as this is the major source of excess calories. Study: The methanolic extract from the leaves of Ilex paraguariensis (Aquifoliaceae) was found to show an inhibitory activity on porcine pancreatic lipase. From the methanolic extract, three new triterpene oligoglycosides, mateglycosides A, B, and C, were isolated together with 18 known compounds. The chemical structures of new oligoglycosides were elucidated on the basis of chemical and physicochemical evidence. Several constituents showed inhibitory activities on ...
Sept. 19, 1950 w. A. BRUCE OIL-WATER SEPARATOR FOR WELLS ATTORNEY. j} INVENTOR. 4 Z s A 9 1 e I n H J F d e l 1 F 2 2 3 l 4 L: a Patented Sept. 19, 1950 I I OIL-WATER SEPARATOR FOR WELLS William A. Bruce, Tulsa, Okla;, assignor to Standard Oil Development Company, a corporation of Delaware a Appli atin nner, 1945, seria No. 59in)? j i f Thepresent invention is directed to apparatus for separating oil from water in a well producing oil and water. The principal object of the present invention istheprovision of apparatus which will make I possible a separation ofoil from water at the pointofjentry of a mixture of the two from a formation containing them into a bore hole, and the separatewithdrawal of these liquids from the bore hole. l I An additionarobject of thepresent invention is to provide at thepoint of" entry of a mixture or oil and water into aborehole from aforma :tioncontainin-g the two a preliminary separation zone, a path of egress to the surface for the oil, Which is ...
Multiscale modeling plays an important role in the study of soft matter systems (polymers, colloids, surfactants). We will discuss a new mesoscopic bead-field hybrid method and its application to specific industrial problems. The method combines dynamic density functional theory, a field based model developed for polymer materials, with particle representations of molecules present at low concentrations (such as surfactant). Coupling between the bead and field components is designed to correctly reproduce thermodynamic behavior. We will presents results using this model to study: (i) the structure and release properties of poly(lactic acid) and poly(lactic-glycolic acid) copolymer microspheres for use in drug delivery and (ii) the design of long chain amphiphilic linker molecules that strongly segregate at oil-water interfaces and are highly effective in stabilizing microemulsions in oil recovery.
Featured as Analyst Hot Article. Maynard, B. A.; Brooks, J. C.; Hardy, E. E.; Easley, C. J.; Gorden, A. E. V.* "Synthesis, structural characterization, electronic spectroscopy, and microfluidic detection of Cu+2 and UO2+2 [di-tert-butyl-salphenazine] complexes," Dalton Trans. 2015, 44, 4428-4430. PDF. Hu, J.; Yu, Y.; Brooks, J. C.; Godwin, L. A.; Somasundaram, S.; Torabinejad, F.; Kim, J.; Shannon, C.*; Easley, C. J.* "A Reusable Electrochemical Proximity Assay for Highly Selective, Real-Time Protein Quantitation in Biological Matrices," J. Am. Chem. Soc. 2014, 136, 8467-8474. PDF. DeJournette, C. J.; Kim, J.; Medlen, H.; Li, X.; Vincent, L. J.; Easley, C. J. "Creating Biocompatible Oil-Water Interfaces without Synthesis: Direct Interactions between Primary Amines and Carboxylated Perfluorocarbon Surfactants." Anal. Chem. 2013, 85, 10556-10564. PDF. Godwin, L. A.; Deal, K. S.; Hoepfner, L. D.; Jackson, L. C.; Easley, C. J. "Measurement of Microchannel Fluidic Resistance with a Standard Voltage ...
extracellular region, extracellular space, lipase activity, triglyceride lipase activity, lipid digestion, lipid metabolic process, positive regulation of triglyceride lipase activity, retinoid metabolic process
lipase to help optimize the digestion of fat, minimizing fat intolerance. Add Lipase Enhance with another of our digestive formulas for additional fat digesting support
Normal lipase test results range between 10 and 140 units per liter for adults 60 and younger and between 18 and 180 units per liter for adults over 60, according to WebMD. The normal ranges may...
Supramolecular assembly at an oil-water interface is an effective way to produce 2D nanomaterials from peptoids because that interface helps pre-organize the peptoid chains to facilitate their self-interaction," says Ron Zuckermann, a senior scientist at the Molecular Foundry, a DOE nanoscience center hosted at Berkeley Lab. "This increased understanding of the peptoid assembly mechanism should enable us to scale-up to produce large quantities, or scale- down to screen many different nanosheets for novel functions.". Zuckermann, who directs the Molecular Foundrys Biological Nanostructures Facility, and Geraldine Richmond of the University of Oregon are the corresponding authors of a paper reporting these results in the Proceedings of the National Academy of Sciences (PNAS). The paper is titled "Assembly and molecular order of two-dimensional peptoid nanosheets at the oil-water interface." Co-authors are Ellen Robertson, Gloria Olivier, Menglu Qian and Caroline Proulx.. Peptoids are synthetic ...
An automated colorimetric method for determining lipase activity in canine sera was evaluated for precision, linearity and correlation to existing assay methods. The colorimetric method was a commercial reagent that used a series of enzymatic reactions based on the hydrolysis of 1,2 diglyceride by pancreatic lipase. Within-run and between-run coefficients of variation were 7 y) dogs using this assay. It was concluded that the automated colorimetric assay was a reliable indicator of canine serum Show moreAn automated colorimetric method for determining lipase activity in canine sera was evaluated for precision, linearity and correlation to existing assay methods. The colorimetric method was a commercial reagent that used a series of enzymatic reactions based on the hydrolysis of 1,2 diglyceride by pancreatic lipase. Within-run and between-run coefficients of variation were 7 y) dogs using this assay. It was concluded that the automated colorimetric assay was a reliable indicator of canine serum ...
The digestion process begins when you start chewing your food.. Your teeth break the food into smaller pieces, and your saliva moistens the food so that its easier for it to move through your esophagus and into your stomach. Your saliva also contains enzymes that begin breaking down the fat in your food.. 2. Esophagus. When you swallow, a series of muscle contractions called peristalsis moves the food through your esophagus and into your stomach.. 3. Stomach. Your stomach lining produces acids and enzymes that break down your food further so that the foods can pass to the small intestine.. 4. Small intestine. The majority of fat digestion happens once it reaches the small intestine. This is also where the majority of nutrients are absorbed.. Your pancreas produces enzymes that break down fats, carbohydrates, and proteins.. Your liver produces bile that helps you digest fats and certain vitamins. This bile is stored in the gallbladder. These digestive juices are delivered to your small intestine ...
Proteomic workflows rely on sensitive and precise methods for purifying and detecting proteins, and one of the most popular methods is liquid chromatography (LC) followed by an inline detector. However, the most commonly used method, UV-Vis absorbance, provides relatively low sensitivity and requires long path lengths in the flow cell. In this thesis, we present a highly sensitive, label-free method to detect proteins in continuous flow, using a pressure-driven microfluidic droplet generator. The system consists of a cross junction or flow focusing structure where the flowing stream of proteins is combined with two coflowing streams of oil which break up the former stream into a train of droplets. In such a system, the droplet size predictably scales with interfacial tension (IFT) of the oil-water interface. When globular proteins (eg BSA) enter the junction, they adsorb to the interface and reduce IFT, and thereby the droplet radius. Temporal variations in droplet size therefore correlate with
Orlistat is one of the most effective drugs for the treatment of obesity. Efficacy was proven by numerous clinical studies, so Orlistat received unconditional support from the medical specialists.. The main active ingredient within the composition of this drug is orlistat. This substance is successfully used in the therapeutic treatment of people suffering from obesity. A distinctive feature of the drug is its ability not to absorb into the blood, and to perform its function of reducing weight immediately in the stomach and small intestine. It is well known that fats that human consumes, are broken down by gastric and pancreatic lipase. Orlistat acts on the lipase, blocking and inhibiting its function. As a result, fat is not deposited in body tissues and is not a source of energy, but it directly excreted during defecation.. It should be particularly noted: this drug is not suitable for a person who just wants to lose 2 - 3 pounds and can adjust the weight. It is sufficient to increase physical ...
This test measures the amount of lipase in your blood. Lipase is an enzyme that is made by your pancreas. Higher levels may mean you have a problem with your pancreas.
https://doi.org/10.18632/oncotarget.23619 Dominique Lombardo, Françoise Silvy, Isabelle Crenon, Emmanuelle Martinez, Aurélie Collignon, Evelyne Beraud, Eric Mas
Fat is an important energy source from food. More than 95% of dietary fat is long-chain triacylglycerols (TAG), the remaining being phospholipids (4.5%) and sterols. In the small intestine lumen, dietary TAG is hydrolyzed to fatty acids (FA) and monoacylglycerols (MAG) by pancreatic lipase. These products are then emulsified with the help of phospholipids (PL) and bile acids (BA) present in bile to form micelles. Free FAs and MAGs are taken up by the enterocyte where they are rapidly resynthesized in endoplasmic reticulum (ER) to form TAG. PLs from the diet as well as bile - mainly LPA - too are absorbed by the enterocyte and are acylated to form phosphatidic acid (PA), which is also converted into TAG. Absorbed cholesterol (CL) is acylated to cholesterol esters (CE). Within the ER, TAG joins CE and apolipoprotein B (ApoB) to form chylomicrons that enter circulation through the lymph ...
Abbate, M., Potze, R., Sawatzky, G.A., Schlenker, C., Lin, H.J., Tjeng, L.H., Chen, C.T., Teehan, D. and Turner, T.S. (1995) Changes in the electronic structure of Ti4O7 across the semiconductor-semiconductor-metal transitions. Physical Review B, 51 (15). pp. 10150-10153. ISSN 1098-0121 Abbate, M., Potze, R., Sawatzky, G.A., Schlenker, C., Teehan, D. and Turner, Tracy (1995) The electronic structure of Ti4O7 studied by resonant photoemission. Solid State Communications, 94 (6). pp. 465-469. ISSN 0038-1098 Alba, K. and Kontogiorgos, Vassilis (2016) Pectin at the oil-water interface: Relationship of molecular composition and structure to functionality. Food Hydrocolloids. ISSN 0268-005X Andreu, J.M., Bordas, Joan, Diaz, J.F., Deancos, J.G., Gil, R., Medrano, Francisco J., Nogales, Eva, Pantos, E. and Towns-Andrews, Elizabeth (1992) Low resolution structure of microtubules in solution: Synchrotron X-ray scattering and electron microscopy of taxol-induced microtubules assembled from purified tubulin ...
Human gastric lipase (hGL) is a member of the family of preduodenal lipases, primarily responsible for initiation of lipolysis of dietary fats, such as triglycerides in the gastrointestinal tract. It is a candidate for treatment of pancreatic enzyme insufficiency, as a superior substitute for the current treatment that uses enzyme obtained from porcine pancreatic extract. Recently, it was demonstrated that active recombinant hGL can be produced in N. benthamiana by transient expression using the CPMV-HT system.. Enzyme assays confirmed that the plant-produced hGL was active. The figure below shows results of an assay conducted to detect the presence of lipase activity in clarified leaf extracts (performed at pH 4.0 using MU-oleate as a substrate). It was seen that extracts from leaves infiltrated with either the wt-hGL or hGL-His pEAQ-HT-based constructs had the ability to release the MU moiety from the MU-oleate substrate, demonstrating that the plant-produced lipase was active. ...
Background: Pancreatitis is a common, debilitating disease in dogs. The non-invasive diagnosis of canine pancreatitis remains a challenge despite the start of abdominal ultrasound and pancreas-specific blood tests. The pancreatic lipase immunoreactivity (PLI) test was developed to address these concerns. A modification of this test, the SPEC cPL, is currently marketed as a non-invasive diagnostic test for canine pancreatitis. Interpretation of abnormal SPEC cPL test results in the absence of other supportive signs or diagnostic tests has been problematic. Objective: The purpose of this study is to assess exocrine pancreatic lesions in the dog and compare the severity of lesions or lack thereof with changes in SPEC cPL and other commonly performed blood tests.
Highly moisturizing and nourishing anti-wrinkle ampoule contains honey and propolis for moisturized and tightened skin.. • 30% of propolis extract protects skin from the external environment and immerses the skin in nutrients as it energizes and tightens.. • Canola honey extract from Jeju balances the skin oil-water content.. • Natural moisturizing ingredients including hyaluronic acid and trehalose with honey ensures all day moisture.. ...
Highly moisturizing and nourishing anti-wrinkle ampoule contains honey and propolis for moisturized and tightened skin.. • 30% of propolis extract protects skin from the external environment and immerses the skin in nutrients as it energizes and tightens.. • Canola honey extract from Jeju balances the skin oil-water content.. • Natural moisturizing ingredients including hyaluronic acid and trehalose with honey ensures all day moisture.. ...
Lipase is an enzyme that helps you digest fats. Lipase supplements can be an excellent support for the discomfort, gas, and bloating from fats in your foods. Heres how lipase supplements work.
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Nisin, an antimicrobial peptide showing activity against many Gram positive bacteria, is widely used as a food preservative. The simulated gastrointestinal digestion of nisin (variant A) was studied using the in vitro INFOGEST digestion method. Following oral, gastric and small intestinal digestion, there was no intact nisin in the system and the nisin was primarily digested by pancreatin. After digestion, six nisin fragments (1-11, 1-12, 1-20, 1-21, 1-29 and 1-32) were identified by reversed phase high performance liquid chromatography and mass spectroscopy and four of these nisin fragments (1-20, 1-21, 1-29 and 1-32) demonstrated low antibacterial activity against Lactococcus lactis HP in agar diffusion activity assays. Additionally, it was observed that bile salts form a complex with nisin. This was examined by atomic force microscopy, turbidity and dynamic light scattering, which showed that this interaction resulted in significantly larger bile salt micelles. The presence of bile salts at ...
Understanding how particles pack on curved surfaces is important for applications in medicine and the food industry in which colloidal particles are embedded, for example, on oil-water interfaces in emulsions. Packing spherical particles on a curved interface is bound to result in a configuration with defects. On a flat surface, particles tend to pack in an orderly hexagonal lattice. However, the presence of curvature makes this type of configuration impossible to obtain, and results in particles having more or fewer than six neighbors. These regions which deviate from a hexagonal lattice are referred to as defects.. Pictured here are configurations of spherical particles packed on ellipsoidal surfaces. Red particles have six neighboring particles, and defects are shown as blue particles which have five neighbors, green particles which have seven neighbors, and occasionally yellow particles which have eight neighbors. The black lines indicate the axis about which the ellipsoidal surfaces are ...
Process vessel has combination single-sweep agitator with pitched mixing bars and secondary mixer. Scrapers on sweep frame keep inside of vessel walls clean while pushing batch toward center, while bars cause turbulence or mixing in batch itself. Bottom-entry mixer is high-shear disperser that blends hard-to-wet powders or oil-water type mixtures. Vacuum/pressure capability allows for both... Read More » ...
Lipase is the name of the enzyme that digests fats. Although the stomach produces small quantities of gastric lipase, the pancreas is the main location for the production of lipase known as...
The histidine-tagged lipases from the culture supernatant were purified using Ni-NTA chromatography by ÄKTA purifier (GE Co.). Lipases ...
... bird or marine colipases. CoSPL was found to be less effective activator of bird and mammal pancreatic lipases than for the ...
Colipases at the US National Library of Medicine Medical Subject Headings (MeSH) This article incorporates text from the public ...
Pancreatic lipases and their complexes with colipases and inhibitors: crystallization and crystal packing. (1997) ... Pancreatic lipases and their complexes with colipases and inhibitors: Crystallization and crystal packing (1998) ...
Pancreatic lipases and their complexes with colipases and inhibitors: crystallization and crystal packing. (1997) ... Pancreatic lipases and their complexes with colipases and inhibitors: Crystallization and crystal packing (1998) ...
Pancreatic lipases and their complexes with colipases and inhibitors: crystallization and crystal packing. (1997) ... Pancreatic lipases and their complexes with colipases and inhibitors: Crystallization and crystal packing (1998) ...
Colipases. 2. Cognition Disorders. 2. Clozapine. 2. Circadian Rhythm. 2. Child Development. ...
Define Coliseum (theatre). Coliseum (theatre) synonyms, Coliseum (theatre) pronunciation, Coliseum (theatre) translation, English dictionary definition of Coliseum (theatre). n. Variant of coliseum. n a variant spelling of coliseum n an amphitheatre in Rome built about 75-80 ad n. 1. an ancient amphitheater in Rome, begun A.D....
Define colinear. colinear synonyms, colinear pronunciation, colinear translation, English dictionary definition of colinear. adj. 1. Collinear. 2. Genetics a. Characterized by correspondence between the linear sequence of nucleotides in a gene and the linear sequence of amino...
Colipases (0) * Contractile Proteins (1) * Cystatins (0) * Cystine-Knot Miniproteins (0) * Cytoskeletal Proteins (0) ...
Colipases (0) * Contractile Proteins (1) * Cystatins (0) * Cystine-Knot Miniproteins (0) * Cytoskeletal Proteins (0) ...
Colipases (0) * Contractile Proteins (1) * Cystatins (0) * Cystine-Knot Miniproteins (0) * Cytoskeletal Proteins (0) ...
Colipases (0) * Contractile Proteins (1) * Cystatins (0) * Cystine-Knot Miniproteins (0) * Cytoskeletal Proteins (0) ...
Colipases (0) * Contractile Proteins (1) * Cystatins (0) * Cystine-Knot Miniproteins (0) * Cytoskeletal Proteins (0) ...
Colipases (0) * Contractile Proteins (1) * Cystatins (0) * Cystine-Knot Miniproteins (0) * Cytoskeletal Proteins (0) ...
": "http://scigraph.springernature.com/ontologies/subjects/colipases" }, { "id": "http://scigraph.springernature.com/ontologies ...
... bird or marine colipases. CoSPL was found to be less effective activator of bird and mammal pancreatic lipases than for the ... the activation of SPL by colipases from other species and the capacity of CoSPL to activate various pancreatic lipases. (A) ... Table 3 Kinetic parameters between stingray lipase and colipases of various species deduced from figure 4A. Full size table ... Only bird colipases reacted strongly with anti CoOPL (Figure. 1B). No cross-immunoreactivity was detected between anti-CoOPL ...
Q: How do you activate colipases?. A: by chymotrypsin, which cleaves the pre-colipase. ...
Proteins and peptides that are involved in SIGNAL TRANSDUCTION within the cell. Included here are peptides and proteins that regulate the activity of TRANSCRIPTION FACTORS and cellular processes in response to signals from CELL SURFACE RECEPTORS. Intracellular signaling peptide and proteins may be part of an enzymatic signaling cascade or act through binding to and modifying the action of other signaling factors ...
A single-chain polypeptide derived from bovine tissues consisting of 58 amino-acid residues. It is an inhibitor of proteolytic enzymes including CHYMOTRYPSIN; KALLIKREIN; PLASMIN; and TRYPSIN. It is used in the treatment of HEMORRHAGE associated with raised plasma concentrations of plasmin. It is also used to reduce blood loss and transfusion requirements in patients at high risk of major blood loss during and following open heart surgery with EXTRACORPOREAL CIRCULATION. (Reynolds JEF(Ed): Martindale: The Extra Pharmacopoeia (electronic version). Micromedex, Inc, Englewood, CO, 1995 ...
Kanazawa T, Chana G, Glatt SJ, Mizuno H, Masliah E, Yoneda H, Tsuang MT, Everall IP. The utility of SELENBP1 gene expression as a biomarker for major psychotic disorders: replication in schizophrenia and extension to bipolar disorder with psychosis. Am J Med Genet B Neuropsychiatr Genet. 2008 Sep 05; 147B(6):686-9 ...
Proteins whose abnormal expression (gain or loss) are associated with the development, growth, or progression of NEOPLASMS. Some neoplasm proteins are tumor antigens (ANTIGENS, NEOPLASM), i.e. they induce an immune reaction to their tumor. Many neoplasm proteins have been characterized and are used as tumor markers (TUMOR MARKERS, BIOLOGICAL) when they are detectable in cells and body fluids as monitors for the presence or growth of tumors. Abnormal expression of ONCOGENE PROTEINS is involved in neoplastic transformation, whereas the loss of expression of TUMOR SUPPRESSOR PROTEINS is involved with the loss of growth control and progression of the neoplasm ...
Wühr M, Güttler T, Peshkin L, McAlister GC, Sonnett M, Ishihara K, Groen AC, Presler M, Erickson BK, Mitchison TJ, Kirschner MW, Gygi SP. The Nuclear Proteome of a Vertebrate. Curr Biol. 2015 Oct 19; 25(20):2663-71 ...
Colipases Research. Contractile Proteins Research. Cystatins Research. Cytoskeletal Proteins Research. Dental Enamel Proteins ...
Looking for online definition of colinearity in the Medical Dictionary? colinearity explanation free. What is colinearity? Meaning of colinearity medical term. What does colinearity mean?

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