In eukaryotes, a genetic unit consisting of a noncontiguous group of genes under the control of a single regulator gene. In bacteria, regulons are global regulatory systems involved in the interplay of pleiotropic regulatory domains and consist of several OPERONS.
Proteins obtained from ESCHERICHIA COLI.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
Proteins found in any species of bacterium.
Infections with bacteria of the species ESCHERICHIA COLI.
The functional hereditary units of BACTERIA.
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A protein which is a subunit of RNA polymerase. It effects initiation of specific RNA chains from DNA.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A species of gram-negative, rod-shaped bacteria belonging to the K serogroup of ESCHERICHIA COLI. It lives as a harmless inhabitant of the human LARGE INTESTINE and is widely used in medical and GENETIC RESEARCH.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Genes which regulate or circumscribe the activity of other genes; specifically, genes which code for PROTEINS or RNAs which have GENE EXPRESSION REGULATION functions.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.
Proteins which maintain the transcriptional quiescence of specific GENES or OPERONS. Classical repressor proteins are DNA-binding proteins that are normally bound to the OPERATOR REGION of an operon, or the ENHANCER SEQUENCES of a gene until a signal occurs that causes their release.
Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
Thin, filamentous protein structures, including proteinaceous capsular antigens (fimbrial antigens), that mediate adhesion of E. coli to surfaces and play a role in pathogenesis. They have a high affinity for various epithelial cells.
A species of gram-positive bacteria that is a common soil and water saprophyte.
A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.
Proteins isolated from the outer membrane of Gram-negative bacteria.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
An error-prone mechanism or set of functions for repairing damaged microbial DNA. SOS functions (a concept reputedly derived from the SOS of the international distress signal) are involved in DNA repair and mutagenesis, in cell division inhibition, in recovery of normal physiological conditions after DNA repair, and possibly in cell death when DNA damage is extensive.
A serotype of Salmonella enterica that is a frequent agent of Salmonella gastroenteritis in humans. It also causes PARATYPHOID FEVER.
The in vitro fusion of GENES by RECOMBINANT DNA techniques to analyze protein behavior or GENE EXPRESSION REGULATION, or to merge protein functions for specific medical or industrial uses.
The genetic complement of a BACTERIA as represented in its DNA.
A species of gram-negative, rod-shaped bacteria isolated from the intestinal tract of swine, poultry, and man. It may be pathogenic.
A dextrodisaccharide from malt and starch. It is used as a sweetening agent and fermentable intermediate in brewing. (Grant & Hackh's Chemical Dictionary, 5th ed)
A transcriptional regulator in prokaryotes which, when activated by binding cyclic AMP, acts at several promoters. Cyclic AMP receptor protein was originally identified as a catabolite gene activator protein. It was subsequently shown to regulate several functions unrelated to catabolism, and to be both a negative and a positive regulator of transcription. Cell surface cyclic AMP receptors are not included (CYCLIC AMP RECEPTORS), nor are the eukaryotic cytoplasmic cyclic AMP receptor proteins, which are the regulatory subunits of CYCLIC AMP-DEPENDENT PROTEIN KINASES.
Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.
The rate dynamics in chemical or physical systems.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Diffusible gene products that act on homologous or heterologous molecules of viral or cellular DNA to regulate the expression of proteins.
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The complete absence, or (loosely) the paucity, of gaseous or dissolved elemental oxygen in a given place or environment. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Proteins prepared by recombinant DNA technology.
Inorganic salts of phosphoric acid.
Mutagenesis where the mutation is caused by the introduction of foreign DNA sequences into a gene or extragenic sequence. This may occur spontaneously in vivo or be experimentally induced in vivo or in vitro. Proviral DNA insertions into or adjacent to a cellular proto-oncogene can interrupt GENETIC TRANSLATION of the coding sequences or interfere with recognition of regulatory elements and cause unregulated expression of the proto-oncogene resulting in tumor formation.
The genetic unit consisting of three structural genes, an operator and a regulatory gene. The regulatory gene controls the synthesis of the three structural genes: BETA-GALACTOSIDASE and beta-galactoside permease (involved with the metabolism of lactose), and beta-thiogalactoside acetyltransferase.
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
The regulatory elements of an OPERON to which activators or repressors bind thereby effecting the transcription of GENES in the operon.
A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.
A whiplike motility appendage present on the surface cells. Prokaryote flagella are composed of a protein called FLAGELLIN. Bacteria can have a single flagellum, a tuft at one pole, or multiple flagella covering the entire surface. In eukaryotes, flagella are threadlike protoplasmic extensions used to propel flagellates and sperm. Flagella have the same basic structure as CILIA but are longer in proportion to the cell bearing them and present in much smaller numbers. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
Ability of a microbe to survive under given conditions. This can also be related to a colony's ability to replicate.
Substances that reduce the growth or reproduction of BACTERIA.
Membrane proteins whose primary function is to facilitate the transport of molecules across a biological membrane. Included in this broad category are proteins involved in active transport (BIOLOGICAL TRANSPORT, ACTIVE), facilitated transport and ION CHANNELS.
Any method used for determining the location of and relative distances between genes on a chromosome.
Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.
An electrophoretic technique for assaying the binding of one compound to another. Typically one compound is labeled to follow its mobility during electrophoresis. If the labeled compound is bound by the other compound, then the mobility of the labeled compound through the electrophoretic medium will be retarded.
A constellation of responses that occur when an organism is exposed to excessive heat. Responses include synthesis of new proteins and regulation of others.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
A DNA-directed RNA polymerase found in BACTERIA. It is a holoenzyme that consists of multiple subunits including sigma factor 54.
Life or metabolic reactions occurring in an environment containing oxygen.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Process of generating a genetic MUTATION. It may occur spontaneously or be induced by MUTAGENS.
Physicochemical property of fimbriated (FIMBRIAE, BACTERIAL) and non-fimbriated bacteria of attaching to cells, tissue, and nonbiological surfaces. It is a factor in bacterial colonization and pathogenicity.
A metallic element with atomic symbol Fe, atomic number 26, and atomic weight 55.85. It is an essential constituent of HEMOGLOBINS; CYTOCHROMES; and IRON-BINDING PROTEINS. It plays a role in cellular redox reactions and in the transport of OXYGEN.
Proteins found in the PERIPLASM of organisms with cell walls.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Strains of ESCHERICHIA COLI characterized by attaching-and-effacing histopathology. These strains of bacteria intimately adhere to the epithelial cell membrane and show effacement of microvilli. In developed countries they are associated with INFANTILE DIARRHEA and infantile GASTROENTERITIS and, in contrast to ETEC strains, do not produce ENDOTOXINS.
Proteins which are synthesized in eukaryotic organisms and bacteria in response to hyperthermia and other environmental stresses. They increase thermal tolerance and perform functions essential to cell survival under these conditions.
Strains of ESCHERICHIA COLI with the ability to produce at least one or more of at least two antigenically distinct, usually bacteriophage-mediated cytotoxins: SHIGA TOXIN 1 and SHIGA TOXIN 2. These bacteria can cause severe disease in humans including bloody DIARRHEA and HEMOLYTIC UREMIC SYNDROME.
Thin, hairlike appendages, 1 to 20 microns in length and often occurring in large numbers, present on the cells of gram-negative bacteria, particularly Enterobacteriaceae and Neisseria. Unlike flagella, they do not possess motility, but being protein (pilin) in nature, they possess antigenic and hemagglutinating properties. They are of medical importance because some fimbriae mediate the attachment of bacteria to cells via adhesins (ADHESINS, BACTERIAL). Bacterial fimbriae refer to common pili, to be distinguished from the preferred use of "pili", which is confined to sex pili (PILI, SEX).
Viruses whose host is Escherichia coli.
Strains of ESCHERICHIA COLI that produce or contain at least one member of either heat-labile or heat-stable ENTEROTOXINS. The organisms colonize the mucosal surface of the small intestine and elaborate their enterotoxins causing DIARRHEA. They are mainly associated with tropical and developing countries and affect susceptible travelers to those places.
Proteins that bind to and are involved in the metabolism of phosphate ions.
Periplasmic proteins that scavenge or sense diverse nutrients. In the bacterial environment they usually couple to transporters or chemotaxis receptors on the inner bacterial membrane.
Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).
A parasexual process in BACTERIA; ALGAE; FUNGI; and ciliate EUKARYOTA for achieving exchange of chromosome material during fusion of two cells. In bacteria, this is a uni-directional transfer of genetic material; in protozoa it is a bi-directional exchange. In algae and fungi, it is a form of sexual reproduction, with the union of male and female gametes.
The heritable modification of the properties of a competent bacterium by naked DNA from another source. The uptake of naked DNA is a naturally occuring phenomenon in some bacteria. It is often used as a GENE TRANSFER TECHNIQUE.
Transport proteins that carry specific substances in the blood or across cell membranes.
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.
A method for determining the sequence specificity of DNA-binding proteins. DNA footprinting utilizes a DNA damaging agent (either a chemical reagent or a nuclease) which cleaves DNA at every base pair. DNA cleavage is inhibited where the ligand binds to DNA. (from Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
A phenomenon where microorganisms communicate and coordinate their behavior by the accumulation of signaling molecules. A reaction occurs when a substance accumulates to a sufficient concentration. This is most commonly seen in bacteria.
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Proteins that are structural components of bacterial fimbriae (FIMBRIAE, BACTERIAL) or sex pili (PILI, SEX).
The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Strains of Escherichia coli that preferentially grow and persist within the urinary tract. They exhibit certain virulence factors and strategies that cause urinary tract infections.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Strains of ESCHERICHIA COLI that are a subgroup of SHIGA-TOXIGENIC ESCHERICHIA COLI. They cause non-bloody and bloody DIARRHEA; HEMOLYTIC UREMIC SYNDROME; and hemorrhagic COLITIS. An important member of this subgroup is ESCHERICHIA COLI O157-H7.
A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.
Excrement from the INTESTINES, containing unabsorbed solids, waste products, secretions, and BACTERIA of the DIGESTIVE SYSTEM.
A species of gram-negative, aerobic, rod-shaped bacteria commonly isolated from clinical specimens (wound, burn, and urinary tract infections). It is also found widely distributed in soil and water. P. aeruginosa is a major agent of nosocomial infection.
The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Enzymes that catalyze the epimerization of chiral centers within carbohydrates or their derivatives. EC 5.1.3.
A LEUCINE and DNA-binding protein that is found primarily in BACTERIA and ARCHAEA. It regulates GENETIC TRANSCRIPTION involved in METABOLISM of AMINO ACIDS in response to the increased concentration of LEUCINE.
The interference in synthesis of an enzyme due to the elevated level of an effector substance, usually a metabolite, whose presence would cause depression of the gene responsible for enzyme synthesis.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
The ability of microorganisms, especially bacteria, to resist or to become tolerant to chemotherapeutic agents, antimicrobial agents, or antibiotics. This resistance may be acquired through gene mutation or foreign DNA in transmissible plasmids (R FACTORS).
Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.
An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.
Any salt or ester of glycerophosphoric acid.
A rather large group of enzymes comprising not only those transferring phosphate but also diphosphate, nucleotidyl residues, and others. These have also been subdivided according to the acceptor group. (From Enzyme Nomenclature, 1992) EC 2.7.
A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).
lac operon Weickert, M. J.; Adhya, S. (October 1993). "The galactose regulon of Escherichia coli". Molecular Microbiology. 10 ( ... Lee, Sang Jun; Lewis, Dale E. A.; Adhya, Sankar (December 2008). "Induction of the Galactose Enzymes in Escherichia coli Is ... Adhya, S.; Miller, W. (1979-06-07). "Modulation of the two promoters of the galactose operon of Escherichia coli". Nature. 279 ... The gal operon of E. coli consists of 4 structural genes: galE (epimerase), galT (galactose transferase), galK (galactokinase ...
Weickert MJ, Adhya S (1993). "The galactose regulon of Escherichia coli" (PDF). Mol. Microbiol. 10 (2): 245-51. doi:10.1111/j. ... In Escherichia coli, cyclic AMP receptor protein (CRP) can regulate the transcription of more than 100 genes. The signal to ...
The L-L-Glycerophosphate Regulon in Escherichia coli (PhD thesis). Harvard University. OCLC 76982917. ProQuest 302206095. Nuzzo ...
... coli Zur regulon". PLOS Biology. 12 (11): e1001987. doi:10.1371/journal.pbio.1001987. PMC 4219657. PMID 25369000. Blindauer CA ... "Severe zinc depletion of Escherichia coli: roles for high affinity zinc binding by ZinT, zinc transport and zinc-independent ...
Larson TJ, Ehrmann M, Boos W (1983). "Periplasmic glycerophosphodiester phosphodiesterase of Escherichia coli, a new enzyme of ... the glp regulon". J. Biol. Chem. 258 (9): 5428-32. doi:10.1016/S0021-9258(20)81908-5. PMID 6304089. Biology portal v t e. ...
... coli Zur regulon". PLOS Biology. 12 (11): e1001987. doi:10.1371/journal.pbio.1001987. PMC 4219657. PMID 25369000. Waldron KJ, ... Zur, responsive to zinc; Zur regulates uptake and transport through a regulon involving ZinT and the transporter ZnuABC. Irr, ... "Severe zinc depletion of Escherichia coli: roles for high affinity zinc binding by ZinT, zinc transport and zinc-independent ...
"GntP is the Escherichia coli Fructuronic acid transporter and belongs to the UxuR regulon". Journal of Bacteriology. 186 (22): ... GntT of E. coli is the physiological gluconate permease. The generalized transport reaction catalyzed by proteins of the GntP ... Four of the seven E. coli paralogues have been found to possess active gluconate uptake activity, and one of them (GntW; TC# 2. ... Members of the GntP family include known gluconate permeases of E. coli and Bacillus species such as the D-Gluconate:H+ ...
1,3-Propanediol production by Escherichia coli expressing genes from the Klebsiella pneumoniae dha regulon. , Applied and ... One of his first projects was the engineering of the microbial pathway for 1,3-propanediol in Escherichia coli. This work was ...
"Expression of the pho regulon interferes with induction of the uhpT gene in Escherichia coli K-12". Archives of Microbiology. ... Shattuck-Eidens, Donna M.; Kadner, Robert J. (1981). "Exogenous Induction of the Escherichia coli Hexose Phosphate Transport ... uhp Region and Evidence for a Positive Activator for Expression of the Hexose Phosphate Transport System of Escherichia coli". ...
... is a database of the regulatory network of gene expression in Escherichia coli K-12. RegulonDB also models the ... organization of the genes in transcription units, operons and regulons. A total of 120 sRNAs with 231 total interactions which ... In bacteria, such as E. coli, genes, are regulated by sequence elements in promoters and related binding sites). RegulonDB ... coli. RegulonDB 9.0 includes 184 experimentally determined transcription factors (TFs) as well as 120 computationally predicted ...
... evidence for multiple positive regulators of the phosphate regulon in Escherichia coli". Genetics. 96 (2): 353-66. PMC 1214304 ... In Gram-negative bacteria, such as Escherichia coli (E. coli), alkaline phosphatase is located in the periplasmic space, ... The optimal pH for the activity of the E. coli enzyme is 8.0 while the bovine enzyme optimum pH is slightly higher at 8.5. ... The precise structure and function of the four isozymes (Int in E.coli) are solely geared to supply a source of inorganic ...
Studies of E. coli response to H2O2 have shown that exposure to H2O2 elevated mRNA levels of 140 genes, of which 30 genes are ... SoxRS regulon genes can be regulated by additional factors. At least three known genes including xthA and katE are regulated by ... In E.coli, negative autoregulation of SoxS protein serves as a dampening mechanism for the soxRS redox stress response. ... E. coli cells have revealed similarities to the aging process of higher organisms. The similarities include increased oxidation ...
... the central transcriptional activator of the Escherichia coli maltose regulon". The Journal of Biological Chemistry. 277 (19): ... Peist R, Koch A, Bolek P, Sewitz S, Kolbus T, Boos W (December 1997). "Characterization of the aes gene of Escherichia coli ...
The latter is produced by the lac operon in Escherichia coli. In nature, lactose is found primarily in milk and milk products. ... "Galactose Regulon of Yeast: From Genetics to Systems Biology". Springer Science & Business Media. Retrieved 26 December 2017 ... Galactofuranose Biosynthesis in Escherichia coli K-12:... Journal of Bacteriology, Feb. 1996, p. 1047-1052 ...
... at Class I MarA Regulon Promoters Mediated by Glutamic Acid 89 of the MarA Transcriptional Activator of Escherichia coli". J ... FnrS might be involved in the observed O2 dependent expression of the IscR regulon. Further targets of FnrS are nagZ and sdhA. ... Tjaden B, Saxena RM, Stolyar S, Haynor DR, Kolker E, Rosenow C (September 2002). "Transcriptome analysis of Escherichia coli ... suggests that FnrS might be a more global regulator in Escherichia coli. It is predicted to control several transcription ...
... a new enzyme of the glycerol 3-phosphate regulon of Escherichia coli". Journal of Bacteriology. 182 (19): 5624-7. doi:10.1128/ ...
Genetic control of the 2-keto-3-deoxy-D-gluconate metabolism in Escherichia coli K-12: KDG Regulon », J Bacteriol., (1974) 117 ... coli in the lab of François Stoeber, (student of Jacques Monod) at INSA Lyon. He then joined the National Cancer Institute (Dr ...
SgaT is a 12 TMS protein, possibly very distantly related to the MFS hexuronate permease of Escherichia coli (TC# 2.A.1.14.2), ... Campos E, Aguilar J, Baldoma L, Badia J (November 2002). "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), ... Several organisms have two or more SgaT paralogues, including E. coli, which has three. In some of the homologues found in ... Yew WS, Gerlt JA (January 2002). "Utilization of L-ascorbate by Escherichia coli K-12: assignments of functions to products of ...
The adaptive response is a form of direct DNA repair in E. coli that protects DNA from damage by external agents or by errors ... The adaptive response is mediated by the ada protein (a part of the ada regulon), which covalently transfers alkyl groups from ... Under sustained exposure to low-level treatment with alkylating mutagens, E. coli can adapt to the presence of the mutagen, ... Sedgwick, B., Robins, P., Totty, Nick., Lindahl, Tomas.Functional Domains and Methyl Acceptor Sites of the Escherichia coli Ada ...
The periplasmic protein ZnuA interacts with ZinT, another component of the regulon controlled by Zur, which is also involved in ... Li H, Jogl G (May 2007). "Crystal structure of the zinc-binding transport protein ZnuA from Escherichia coli reveals an ... Patzer SI, Hantke K (June 1998). "The ZnuABC high-affinity zinc uptake system and its regulator Zur in Escherichia coli". ... "Role of ZnuABC and ZinT in Escherichia coli O157:H7 zinc acquisition and interaction with epithelial cells". BMC Microbiology. ...
They are also found in the 5'UTR of htrA (high temperature requirement) genes in Salmonella and E.coli. In V. cholerae fourU ... Permina EA, Gelfand MS (2003). "Heat shock (sigma32 and HrcA/CIRCE) regulons in beta-, gamma- and epsilon-proteobacteria". ... Sigma factor-RNA thermometer combinations have been found to regulate other heat-shock genes (such as ibpA in E. coli) which ... Bukau B (August 1993). "Regulation of the Escherichia coli heat-shock response". Molecular Microbiology. 9 (4): 671-680. doi: ...
archive] "Académie des sciences". Décret du 2 mai 2017 portant promotion et nomination 1.M. Schwartz, "The maltose regulon", in ... an Escherichia coli outer membrane protein", Proc. Natl. Acad. Sci. USA, 75, p. 5802-5806 (1978) 1. F. Moreno, A.V. Fowler, M. ... in the bacterium Escherichia coli. These have enabled him to address very general questions, such as the regulation of protein ... "Isolation of the bacteriophage Lambda receptor from Escherichia coli", J. Bacteriol., 116, p. 1436-1446 (1973) 1. S.D. Emr, M. ...
... coli and in other bacteria. E. coli has a well-studied GltS glutamate/Na+ symporter that aids in the uptake of glutamate into ... The cAMP-CRP complex activates the gal regulon and is responsible for upregulation of GalP (Figure 2) (9,11). GalP is also ... The GalP/H+ symporter is the galactose permease from the galP gene of the Escherichia coli genome. Galactose is an alternate ... 3. McDonald, T. P. and Henderson, P. J. F. (2001) Cysteine residues in the D-galactose-H+ symport protein of Escherichia coli: ...
The latter is produced by the lac operon in Escherichia coli.[citation needed] In nature, lactose is found primarily in milk ... Bhat PJ (2 March 2008). Galactose Regulon of Yeast: From Genetics to Systems Biology. Springer Science & Business Media. ISBN ... February 1996). "Galactofuranose biosynthesis in Escherichia coli K-12: identification and cloning of UDP-galactopyranose ...
"The Per regulon of enteropathogenic Escherichia coli : identification of a regulatory cascade and a novel transcriptional ... Quorum-sensing E. coli regulator A (QseA) is found in LuxS systems and activates transcription of ler. Fis, a nucleoid ... of pathogenic Escherichia coli competes off histone-like nucleoid-structuring protein (H-NS) through noncooperative DNA binding ... and enterohemorrhagic Escherichia coli (EHEC). More specifically, Ler regulates the locus of enterocyte effacement (LEE) ...
Bonocora RP, Smith C, Lapierre P, Wade JT (October 2015). "Genome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, ... "Genome-wide analysis of the RpoN regulon in Geobacter sulfurreducens". BMC Genomics. 10: 331. doi:10.1186/1471-2164-10-331. PMC ... Hunt TP, Magasanik B (December 1985). "Transcription of glnA by purified Escherichia coli components: core RNA polymerase and ... coli σ54 has since been shown to play important regulatory roles in a variety of other cellular processes. Similarly, σ54 ...
In Escherichia coli, the transcription factor, FadR, functions as a switch that co-ordinately regulates the machinery required ... This single [repressor controls the transcription of the whole fad regulon. Binding of fadR is specifically inhibited by long ... Transcriptional control of fatty acid metabolism in Escherichia coli". J. Biol. Chem. 276 (20): 17373-9. doi:10.1074/jbc. ...
... regulon, an Escherichia coli adaptive response protein Adah (disambiguation) Adha (disambiguation) Ada'a, a woreda in the ...
The malE gene, coding for MBP, belongs to the Mal regulon of E. coli, which consists of ten genes whose products are geared for ... MBP is exported into the periplasmic space of E. coli. The NH2-terminal extension of MBP, also termed signal peptide, has two ... MBP is encoded by the malE gene of Escherichia coli. The malE gene codes for a precursor polypeptide (396 amino acid residues) ... All the gene involved in the transport of maltose/maltodextrin, including malE, are clustered in the malB region of E. coli and ...
... coli multidrug efflux pump of the RND superfamily (TC #2.A.6.2)) and the mar regulon itself, and (3) MarB, a small protein of ... The mar locus consists of two divergently positioned transcriptional units that flank the operator, marO, in both E. coli and ... regulates mar regulon expression. Loss of MppA causes overproduction of MarA which activates acrAB, causing pleiotropic drug ... "Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis". Proceedings of ...
Molecular characterization and expression in Escherichia coli of three β-1,3-Glucanase genes from Lysobacter enzymogenes Strain ... the mutant phenotype implies that a broad range of genes is involved in secreted antimicrobials associated with the clp regulon ...
Anti sigma factor and E-coliEdit. As a negative feedback interaction, anti-sigma factor in E-coli, help the cell or allow it to ... "A novel transcriptional regulation mechanism in the flagellar regulon of Salmonella typhimurium: an antisigma factor inhibits ... Anti-sigma factors have been found in a number of bacteria, including Escherichia coli and Salmonella, and in the T4 ... For example, anti-sigma factor 70 Rsd in E. coli is present in the stationary phase and blocks the activity of sigma factor 70 ...
... coli infection, and any patient infected with E. coli O157 was at risk for potentially fatal hemolytic-uremic syndrome.[27] ... coli infection.[25] Potential E. coli infection changed the treatment plan for patients reporting to these hospitals or to ... "E. coli crisis abates, but blame intensifies". The Charleston Gazette. The Associated Press. 28 May 2000. Archived from the ... E. coli O157 was found in at least one sample from the Owen Sound Hospital; when health officials contacted other hospitals in ...
Filamentous E. coli can be up to 70 µm in length and has been identified playing an important role in pathogenesis in human ... The LexA regulon includes a cell division inhibitor, SulA, that prevent the transmission of mutant DNA to the daughter cells. ... Janion, C; Sikora A; Nowosielka A; Grzesiuk E (2003). "E. coli BW535, a triple mutant for the DNA repair genes xthA, nth, nfo, ... Base Excision Repair (BER) mechanism, as a strategy to repair DNA damage observed in E. coli. This involves two types of ...
In E. coli citrate synthase, the enzyme involved in the condensation reaction initiating the Citric Acid Cycle is strongly ... The genes required for the synthesis of cysteine are coded for on the cys regulon. The integration of sulfur is positively ... Feedback inhibition of final products is the main method of inhibition, and, in E. coli, the ilvEDA operon also plays a part in ... This leader sequence is important for the regulation of histidine in E. coli. The his operon operates under a system of ...
Escherichia coli (/ˌɛʃɪˈrɪkiə ˈkoʊlaɪ/; commonly abbreviated E. coli) is a Gram-negative gammaproteobacterium commonly found in ... E. coli genotypes - OpenWetWare *^ Meselson, M; Yuan, R (1968). "DNA restriction enzyme from E. Coli". Nature. 217 (5134): 1110 ... E. coli was one of the first organisms to have its genome sequenced; the complete genome of E. coli K-12 was published by ... E. coli is frequently used as a model organism in microbiology studies. Cultivated strains (e.g. E. coli K-12) are well-adapted ...
In Escherichia coli, replication proceeds unidirectionally from oriV after activation by TrfA. In E. coli, multiple plasmid ... J A Kornacki, C H Chang, and D H Figurski: "kil-kor regulon of promiscuous plasmid RK2: structure, products, and regulation of ... coli W (ATCC 9637): comparative genome analysis and an improved genome-scale reconstruction of E. coli", BMC Genomics, 2011, ... The copy number of RK2 is about 4-7 per cell in E. coli and 3 in P. aeruginosa. Several minimal derivatives of RK2 have been ...
The 245 nucleotide sRNA of Escherichia coli, CsrC, was discovered using a genetic screen for factors that regulate glycogen ... "A direct link between the global regulator PhoP and the Csr regulon in Y. pseudotuberculosis through the small regulatory RNA ... coli during a large scale screen [2]. The gene called SraK, was highly abundant in stationary phase, but low levels could be ... coli. CsrC antagonises the regulatory effects of CsrA, presumably by sequestering this protein. The discovery of CsrC is ...
Silakowski, B.; Kunze, B.; Nordsiek, G.; Blöcker, H.; Höfle, G.; Müller, R. (2000). "The myxochelin iron transport regulon of ... coli colony Type strain of Stigmatella aurantiaca at BacDive - the Bacterial Diversity Metadatabase. ...
Grundy FJ, Henkin TM (November 1998). "The S box regulon: a new global transcription termination control system for methionine ... Franklund CV, Kadner RJ (June 1997). "Multiple transcribed elements control expression of the Escherichia coli btuB gene". ...
The system is especially well characterized in the model organism Escherichia coli, where it is classically described as a ... In bacterial genetics, the mal regulon is a regulon - or group of genes under common regulation - associated with the ... The maltose regulation system in E. coli is a classic example of positive regulation. malT is regulated by catabolite ... Boos, W; Shuman, H (March 1998). "Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation". ...
GcvB has been shown to regulate a large number of genes in E. coli and Salmonella species. GcvB was shown to bind to Oppa and ... "Determination of the small RNA GcvB regulon in the Gram-negative bacterial pathogen Pasteurella multocida and identification of ... It has been shown by gene deletion that GcvB is a regulator of acid resistance in E. coli. GcvB enhances the ability of the ... The GcvB gene is found in enteric bacteria such as Escherichia coli. GcvB regulates genes by acting as an antisense binding ...
She presented a paper entitled The Xanthomonas axonopodis citri PHO regulon. Most media relating to Taschner can be found on ... with a thesis entitled The regulation of alkaline phosphatase by the sigma factor S of RNA polymerase from Escherichia coli. ... "The effect of IHF on sigmaS selectivity of the phoA and pst promoters of Escherichia coli". Archives of Microbiology. 185. ... "The effect of the rpoSam allele on gene expression and stress resistance in Escherichia coli". Archives of Microbiology. ...
RybB is a small non-coding RNA was identified in a large scale screen of Escherichia coli. The function of this short RNA has ... "Conserved small non-coding RNAs that belong to the sigmaE regulon: role in down-regulation of outer membrane proteins". J Mol ... In Escherichia coli, direct interactions for RybB with the following targets have been verified experimentally: b0805 (fiu), ... RNAs endow a transcriptional activator with essential repressor functions for single-tier control of a global stress regulon". ...
First sRNA shown to mediate persistence to antibiotics in E.coli. The finding may lead to discovery of novel treatments for ... Mey AR, Craig SA, Payne SM (September 2005). "Characterization of Vibrio cholerae RyhB: the RyhB regulon and role of ryhB in ... Zhang S, Liu S, Wu N, Yuan Y, Zhang W, Zhang Y (2018). "Escherichia coli by Reducing Cellular Metabolism". Frontiers in ... Massé E, Vanderpool CK, Gottesman S (October 2005). "Effect of RyhB small RNA on global iron use in Escherichia coli". Journal ...
In Escherichia coli, this pathway is well understood. FabA is a β-hydroxydecanoyl-ACP dehydrase - it is specific for the 10- ... It acts as an activator of fabA and fabB transcription and as a repressor for the β-oxidation regulon. In contrast, FabR acts ... The diagrams presented show how fatty acids are synthesized in microorganisms and list the enzymes found in Escherichia coli. ... Elongation of the fatty acid follows the same biosynthetic pathway in Escherichia coli used to produce straight-chain fatty ...
Regulons can sometimes be pathogenicity islands. The Ada regulon in E. coli is a well-characterized example of a group of genes ... The heat shock response in E. coli is regulated by the sigma factor σ32 (RpoH), whose regulon has been characterized as ... "Regulon and promoter analysis of the E. coli heat-shock factor, sigma32, reveals a multifaceted cellular response to heat ... an often-studied example is the phosphate regulon in E. coli, which couples phosphate homeostasis to pathogenicity through a ...
... an integrated system for regulon inference in prokaryotes by comparative genomics approach". Nucleic Acids Research. 38 (Web ... transcriptional regulation of Escherichia coli K-12 integrated within genetic sensory response units (Gensor Units)". Nucleic ...
Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli.. J T ... Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. ... Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. ... Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. ...
The soxR locus of Escherichia coli K12 mediates transcriptional activation of a complex oxidative stress regulon in response to ... Molecular characterization of the soxRS genes of Escherichia coli: two genes control a superoxide stress regulon Nucleic Acids ... The soxR locus of Escherichia coli K12 mediates transcriptional activation of a complex oxidative stress regulon in response to ... DNA sequence analysis revealed the presence of two genes involved in activating the soxR regulon. These genes, named soxR and ...
This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by PhoB ... Escherichia coli O157:H7. Escherichia coli (strain K12). Escherichia coli N37122PS. Escherichia coli TW10598. Escherichia coli ... Escherichia coli N36254PS. Escherichia coli D6-113.11. Escherichia coli NCCP15648. Klebsiella pneumoniae IS22. Escherichia coli ... Escherichia coli (strain K12). Escherichia coli N37122PS. And more. 229. UniRef90_P45607. Cluster: Phosphate regulon ...
Comparison of the PhoPQ regulon in Escherichia coli and Salmonella typhimurium.. Monsieurs P1, De Keersmaecker S, Navarre WW, ... This allowed us to reconstruct the direct PhoPQ-dependent regulons in E. coli K12 and S. typhimurium LT2. Comparison of both ... To verify this hypothesis, we compared the composition of the PhoPQ regulon between E. coli and S. typhimurium using a ... 1989). Compared to nonpathogenic strains, such as E. coli K12, the PhoPQ regulon in pathogens must contain target genes ...
Characterization of the AggR Regulon in Enteroaggregative Escherichia coli. Nicholas Morin, Araceli E. Santiago, Robert K. ... Characterization of the AggR Regulon in Enteroaggregative Escherichia coli. Nicholas Morin, Araceli E. Santiago, Robert K. ... Characterization of the AggR Regulon in Enteroaggregative Escherichia coli. Nicholas Morin, Araceli E. Santiago, Robert K. ... Characterization of the AggR Regulon in Enteroaggregative Escherichia coli Message Subject (Your Name) has forwarded a page to ...
Expression of many of the genes in the SOS regulon are controlled by the LexA protein. LexA acts as a transcriptional repressor ... Exposure of Escherichia coli to a variety of DNA-damaging agents results in the induction of the global SOS response. ... Identification of additional genes belonging to the LexA regulon in Escherichia coli Mol Microbiol. 2000 Mar;35(6):1560-72. doi ... coli LexA regulon to 31. The potential function of each newly identified LexA-regulated gene is discussed. ...
Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA- ... Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA- ... Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA- ... Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA- ...
Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the ... Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the ... Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the ... Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the ...
Microarray experiments show that N. gonorrhoeae FNR controls a much smaller regulon than its E. coli counterpart; it activates ... coli NarX-NarL or NarQ-NarP. A plasmid expressing gonococcal narQ-narP was unable to complement E. coli narQP or narXL mutants ... coli NarL or NarP was confirmed. Despite the sequence similarities between transcription activators of E. coli and N. ... In contrast, FNR acts as both an activator and repressor involved in the control of more than 100 operons in E. coli regulating ...
... targets for the well-studied fumarate nitrate reductase regulator showed the effectiveness of our approach in Escherichia coli ... Outten FW, Outten CE, Hale J, OHalloran TV: Transcriptional activation of an Escherichia coli copper efflux regulon by the ... DISTILLER: a data integration framework to reveal condition dependency of complex regulons in Escherichia coli. ... These methods have been successfully used to infer simple regulons [15-17] or to directly infer complex regulons, that is, the ...
The NsrR protein of Escherichia coli is a transcriptional repressor that contains an [Fe-S] cluster that is the binding site ... f Inefficient translation of nsrR constrains behaviour of the NsrR regulon in Escherichia coli ... Inefficient translation of nsrR constrains behaviour of the NsrR regulon in Escherichia coli, Page 1 of 1 ... The NsrR regulon of Escherichia coli K-12 includes genes encoding the hybrid cluster protein and the periplasmic, respiratory ...
E. coli isolates were selected based on their non-susceptibility to at least one of the carbapenem and E. coli ATCC 25922 was ... Transcriptional crosstalk within the mar/sox/rob regulon in Escherichia coli is limited to the rob and marRAB operons. J ... Two divergently transcribed genes, soxR and soxS, control a superoxide response regulon of Escherichia coli. J Bacteriol. 1991; ... Two-stage induction of the soxRS (superoxide response) regulon of Escherichia coli. J Bacteriol. 1992;174(12):3915-20. ...
However, a comprehensive comparison of OmpR regulons between S. Typhimurium and E. coli is lacking. In this study, we examined ... However, a comprehensive comparison of OmpR regulons between S. Typhimurium and E. coli is lacking. In this study, we examined ... Herein, we classify distinct OmpR regulons and also identify shared OmpR regulatory pathways between S. Typhimurium and E. coli ... Herein, we classify distinct OmpR regulons and also identify shared OmpR regulatory pathways between S. Typhimurium and E. coli ...
In this study, we show that the ,i,E,/i,. ,i,coli ompR malT,/i,,sup,con,/sup, double mutant exhibits a synthetic lethal ... A. Hartmann and W. Boos, "Mutations in phoB, the positive gene activator of the pho regulon in Escherichia coli, affect the ... O. Raibaud and E. Richet, "Maltotriose is the inducer of the maltose regulon of Escherichia coli," Journal of Bacteriology, vol ... C. Chapon, "Role of the catabolite activator protein in the expression of the maltose regulon of Escherichia coli," Annales de ...
Here, the transcript profiles of aerobic glucose-limited steady-state chemostat cultures of E. coli K-12 MG1655, slyA mutant ... Previously, SlyA has been shown to directly regulate only two operons in Escherichia coli K-12 MG1655, fimB and hlyE (clyA). In ... oa Identification of new members of the Escherichia coli K-12 MG1655 SlyA regulon MyBook is a cheap paperback edition of the ... Identification of new members of the Escherichia coli K-12 MG1655 SlyA regulon, Page 1 of 1 ...
The second, designated enn5.8193, expresses an IgG-binding protein when cloned in Escherichia coli. A comparison of enn5.8193 ... Most M type 5 group A streptococcal strains were found to contain a single emm-like gene between virR and scpA (the Vir regulon ... Horizontal gene transfer in the evolution of group A streptococcal emm-like genes: gene mosaics and variation in Vir regulons. ... but two distinct emm-like genes were identified in the Vir regulon of the M5 strain NCTC8193. The complete sequences of both of ...
Figure 3. The expanded regulon of NtrC.. Summary of the processes affected by the adaptive response to N starvation in E. coli ... Figure 2. The Ntr response in E. coli.. The signalling cascade E. coli uses to sense and respond to N starvation, which results ... 28] studied the transcriptome of N-starved E. coli under steady-state conditions (i.e. where the E. coli cells are growing at ... coli (see above), the σ38 regulon was not dysregulated at N+ or N− and only modestly dysregulated at N-24 in mutant bacteria. A ...
CollecTF regulon and TF-binding site reports for selected transcription factors. Provides details on TF-binding motif structure ... H-NS - UniProtKB: P0ACF8 regulon and binding site collection of Escherichia coli str. K-12 substr. MG1655 * Binding sites ... H-NS - UniProtKB: P0ACF8 regulon and binding site collection of Escherichia coli * Binding sites ...
... were mapped at 75.3 min on the Escherichia coli chromosome. A Tn10 insertion in ugpA resulted... ...
Characterization of TetD as a transcriptional activator of a subset of genes of the Escherichia coli SoxS/MarA/Rob regulon.. ... Characterization of TetD as a transcriptional activator of a subset of genes of the Escherichia coli SoxS/MarA/Rob regulon.. ... Amino Acid Sequence, Binding Sites, DNA, DNA-Binding Proteins, Escherichia coli, Escherichia coli Proteins, Gene Expression ... In Escherichia coli, SoxS, MarA and Rob form a closely related subset of the AraC/XylS family of positive regulators, sharing ...
Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12. / Wackett ... Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12. Journal ... title = "Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12", ... T1 - Involvement of the phosphate regulon and the psiD locus in carbon-phosphorus lyase activity of Escherichia coli K-12 ...
Family inner membrane protein, creBC regulon. Escherichia coli. Loading... A0A026V0I1 Uncharacterized protein. Escherichia coli ...
The genes belonging to the AdcR regulon (adcRCBA, adcAII-phtD, phtA, phtB, and phtE) and the PsaR regulon (pcpA, prtA, and ... We have further studied the role of Ni2+ in the regulation of the AdcR regulon by using ICP-MS analysis, electrophoretic ... We have further studied the role of Ni2+ in the regulation of the AdcR regulon by using ICP-MS analysis, electrophoretic ... and the PsaR regulon (pcpA, prtA, and psaBCA) were highly upregulated in the presence of Ni2+. ...
... coli genome tend to minimize the spatial distance between operons in the same regulon. A similar global organization feature ... By renormalizing the GCC data, we compared the interaction frequency of operon pairs in the same regulon with that of random ... This study revealed the organization features of Escherichia coli genomic functional units in the 3D space and furthered our ... The DNA interaction data of Escherichia coli, measured by the genome conformation capture (GCC) technique, have recently become ...
Strikingly, the WhiB regulon is identical to the previously characterized WhiA regulon, providing an explanation for the ... One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640-6645. ... Defining the WhiB regulon.The benefit of using ChIP-seq to determine if WhiB functions as a transcription factor is that any ... Escherichia coli K-12 strain DH5α was used for plasmid and cosmid propagation. BW25113 (53) containing a λ RED plasmid, pIJ790 ...
2001) Characterization of the Escherichia coli sigma E regulon. J Biol Chem 276:20866-20875. ... 1987) Genes encoding two lipoproteins in the leuS-dacA region of the Escherichia coli chromosome. J Bacteriol 169:5692-5699. ... 2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA 97:6640- ... 1988) Mapping and sequencing of mutations in the Escherichia coli rpoB gene that lead to rifampicin resistance. J Mol Biol 202: ...
approximate positions of some of the genes of the SOS regulon are shown. SOS RESPONSE 20 Trans-lesion synthesis by DNA POL V (A ... Regulation of the SOS response regulon in E. coli. (A) About 30 genes around the E. coli chromosome are normally repressed by ... Unformatted text preview: approximate positions of some of the genes of the SOS regulon are shown. SOS RESPONSE 20 Trans-lesion ... DNA Repair I. Methyl-directed mismatch repair system in E coli. a. MMR- mismatch repa ...
The Escherichia coli lac regulon. lacI encodes the lac repressor, which in its active form inhibits the transcription of the ... Kepes F (2004) Periodic transcriptional organization of the E. coli genome. J Mol Biol 340:957-964PubMedCrossRefGoogle Scholar ... Daubin V, Ochman H (2004) Bacterial genomes as new gene homes: the genealogy of ORFans in E. coli. Genome Res 14:1036-1042 ... Studies of the expression patterns of genes in E. coli operons and of orthologous genes in "not-yet" operons in the related ...
DISTILLER: a data integration framework to reveal condition dependency of complex regulons in Escherichia coli. Karen Lemmens, ... DISTILLER: a data integration framework to reveal condition dependency of complex regulons in Escherichia coli. Genome Biology ...
  • By using this heterology index, together with other search criteria, such as the location of the putative LexA box relative to a gene or to promoter elements, we have performed computational searches of the entire E. coli genome to identify novel LexA-regulated genes. (nih.gov)
  • The glpR gene encodes the repressor for the glycerol 3-phosphate regulon, a protein predicted to contain 252 amino acids with a calculated molecular weight of 28,048. (asm.org)
  • Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the soxS regulatory gene. (asm.org)
  • A two-stage control system was hypothesized for soxRS, in which an intracellular redox signal would trigger the SoxR protein as a transcriptional activator of the soxS gene and the resulting increased levels of SoxS protein would activate transcription of the various soxRS regulon genes (B. Demple and C.F. Amábile Cuevas, Cell 67:837-839, 1990). (asm.org)
  • Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection Mol Syst Biol 2 20060008 [CrossRef] [PubMed] . (microbiologyresearch.org)
  • The experiment was performed by using a house keeping gene RpslE as an internal control and the relative C t value of the target genes were compared with that of the control E. coli ATCC 25922 to determine the fold change in the expressional level of mRNA of the test isolates. (biomedcentral.com)
  • Horizontal gene transfer in the evolution of group A streptococcal emm-like genes: gene mosaics and variation in Vir regulons. (nih.gov)
  • Most M type 5 group A streptococcal strains were found to contain a single emm-like gene between virR and scpA (the Vir regulon), but two distinct emm-like genes were identified in the Vir regulon of the M5 strain NCTC8193. (nih.gov)
  • E. coli acquires virulence determinants by horizontal gene transfer as parts of plasmids, bacteriophages, transposons or pathogencity islands, and this process plays a crucial role in the colonization of a new host environment and in the successful establishment of a pathogenic lifestyle [4] , [5] . (plos.org)
  • The effects of fur on the transcriptional and post-transcriptional regulation of MnSOD gene (sodA) in Escherichia coli. (semanticscholar.org)
  • Cabrera-Valladares N, Martínez A, Pinero S, Lagunas-Muñoz VH, Tinoco R, de Anda R, Vazquez-Duhalt R, Bolívar F, Gosset G (2006) Expression of the melA gene from Rhizobium etli CFN42 in Escherichia coli and characterization of the encoded tyrosinase. (springer.com)
  • In addition to PolIV, encoded by the dinB gene, E. coli possesses two more DNA polymerases capable of bypassing lesions: PolV, encoded by the umuDC genes and belonging to the Y-family, and PolII, encoded by the polB gene and belonging to the B-family of DNA polymerases ( N ohmi 2006 ). (genetics.org)
  • Previously, the gene cg0156 was shown to belong to the regulon of McbR, a global transcriptional repressor of sulphur metabolism in C. glutamicum. (sigmaaldrich.com)
  • A comparison of the regulation of type 1 pilus orientation in non-pathogenic and pathogenic E. coli , as a result of fim gene expression. (kenyon.edu)
  • A rapid method for gene expression analysis, PURExpress ® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. (neb.com)
  • The GadX regulon affects virulence gene expression and adhesion of porcine enteropathogenic Escherichia coli in vitro . (uni-giessen.de)
  • The development of fluoroquinolone resistance in Escherichia coli may be associated with mutations in regulatory gene loci such as marRAB that lead to increased multidrug efflux, presumably through activation of expression of the AcrAB multidrug efflux pump. (asm.org)
  • These observations confirm the importance of efflux-associated nontarget gene mutations and indicate that transposition of genetic elements may have a role in the development of fluoroquinolone resistance in E. coli . (asm.org)
  • After fluoroquinolone exposure in vitro, E. coli mutants selected from DNA gyrase single mutants typically exhibit a MDR phenotype with enhanced multidrug efflux but without additional target gene mutations ( 19 ). (asm.org)
  • Strain LH530, a mutant of Escherichia coli K-12, was reported by others to show increased outer membrane permeability, temperature-sensitive growth, and reduced synthesis of lipid A. The unmapped mutant gene was found to be suppressed by high-copy-number plasmids carrying the wild-type acpT gene, which encodes a protein that catalyzes a post-translational protein modification, the attachment of 4′-phosphopantetheine. (genetics.org)
  • Molecular Analysis of RT-like gene which is newly found in Escherichia coli. (nii.ac.jp)
  • We isolated a new reverse transcriptase (RT) -like gene from Escherichia coli K-12 and it is located at 6 min on the E.coli genetic map. (nii.ac.jp)
  • The gene also exists in E.coli C with multicopy and Shigella dysenteriae type I strain, but dose not exist in E.coli B and othe bacterial species investigated. (nii.ac.jp)
  • Publications] Kim,S-K.: 'Molecular analysis of the phoH gene,belonging to the phosphate regulon in Escherichiacoli' J.Bacteriol.175. (nii.ac.jp)
  • Using a multiplex PCR for five SPATE genes ( pet, sat, sigA, pic, and sepA ), we found that most of the Shigella isolates also harbored more than one SPATE, whereas members of most other E. coli pathotypes rarely harbored a cytotoxic SPATE gene. (ajtmh.org)
  • Datenbank-Analysen mit dem E. coli Methioninaufnahmesystem MetD als Vorlage führten zur Identifizierung der Gene metI, metN und metQ in C. glutamicum. (uni-koeln.de)
  • Functional Genomics of E. coli characterizing the genome of E. coli by measuring the expression level of every gene under a variety of environmental and genetic conditions. (davidson.edu)
  • Hence, if periplasmic misfolding occurs in the cell of an E.coli bacteria, the reporter gene, in this case the Red Fluroscent Protein (RFP), will be activated. (igem.org)
  • Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor. (proteopedia.org)
  • The mmuM gene product from Escherichia coli is an archetypal HMT family protein and contains a predicted zinc-binding motif in the enzyme active site. (portlandpress.com)
  • Repressor for the sn-glycerol 3-phosphate regulon of Escherichia coli K-12: primary structure and identification of the DNA-binding domain. (asm.org)
  • The amino acid sequence of the glp repressor was similar to several repressors of carbohydrate catabolic systems, including those of the glucitol (GutR), fucose (FucR), and deoxyribonucleoside (DeoR) systems of E. coli, as well as those of the lactose (LacR) and inositol (IolR) systems of gram-positive bacteria and agrocinopine (AccR) system of Agrobacterium tumefaciens. (asm.org)
  • In contrast, FNR acts as both an activator and repressor involved in the control of more than 100 operons in E. coli regulating major changes in the adaptation from aerobic to anaerobic conditions. (biomedcentral.com)
  • The NsrR protein of Escherichia coli is a transcriptional repressor that contains an [Fe-S] cluster that is the binding site for nitric oxide (NO). Reaction of NsrR with NO leads to de-repression of its target genes, which include those encoding an NO scavenging flavohaemoglobin and the RIC (repair of iron centres) protein involved in the repair of NO-damaged [Fe-S] clusters. (microbiologyresearch.org)
  • The uxuAB operon is under the dual control of uxuR- and exuR-encoded repressors whereas the exu regulon genes are regulated by the sole ExuR repressor. (bireme.br)
  • These occur either in certain regulons where duplication appears to be a requirement for repressor action or in promoters subject to complex regulation. (asm.org)
  • Meng LM, Kilstrup M, Nygaard P. Autoregulation of PurR repressor synthesis and involvement of purR in the regulation of purB, purC, purL, purMN and guaBA expression in Escherichia coli. (proteopedia.org)
  • Rolfes RJ, Zalkin H. Purification of the Escherichia coli purine regulon repressor and identification of corepressors. (proteopedia.org)
  • Choi KY, Zalkin H. Structural characterization and corepressor binding of the Escherichia coli purine repressor. (proteopedia.org)
  • The crystal structures of corepressor-bound and free Escherichia coli purine repressor (PurR) have delineated the roles of several residues in corepressor binding and specificity and the intramolecular signal transduction (allosterism) of this LacI/GalR family member. (proteopedia.org)
  • a) Repression in Escherichia coli of the promoter ( P ) of the catabolic lac operon by binding of LacI repressor (red) with the operator ( O ). Active LacI is inactivated by binding of inducer. (els.net)
  • b) Repression in E. coli of the promoter of the anabolic trp operon by the interaction of TrpR repressor (red) with the operator. (els.net)
  • a) The DNA sequence of the half of Escherichia coli bacteriophage 434 operator (red) and the amino acid sequence of the recognition helix (α2) of the 434 cI repressor (red). (els.net)
  • The Escherichia coli MetR repressor bound to its operator DNA by a β‐fold motif. (els.net)
  • 1989). Compared to nonpathogenic strains, such as E. coli K12, the PhoPQ regulon in pathogens must contain target genes specifically contributing to the virulence phenotype. (nih.gov)
  • AggR is a transcriptional regulator of enteroaggregative Escherichia coli (EAEC) and has been proposed as the defining factor for typical EAEC strains. (asm.org)
  • Multiple epidemiologic studies suggest that strains expressing AggR are more likely to cause diarrheal disease than those without it, leading us to propose the term "typical EAEC" to describe strains harboring the as-yet-incompletely characterized AggR regulon ( 8 , 12 , 13 ). (asm.org)
  • In this study, we examined the expression profiles of wild-type and ompR null strains of the intracellular pathogen S . Typhimurium and a commensal E. coli in response to acid and osmotic stress. (frontiersin.org)
  • Here, the transcript profiles of aerobic glucose-limited steady-state chemostat cultures of E. coli K-12 MG1655, slyA mutant and slyA over-expression strains are reported. (microbiologyresearch.org)
  • These data provide the first clear evidence that the horizontal transfer of emm-like sequences between distinct strains contributes to the evolution of group A streptococcal emm-like genes and Vir regulons. (nih.gov)
  • At the same time, we observe that the content of spermidine and putrescine, a relevant intermediate in the synthesis of spermidine, is higher in all strains as compared to E. coli . (plos.org)
  • Genome analyses clearly confirm that also the loss of genes by means of so-called pathoadaptive mutations has strongly contributed to the emergence of pathogenic E. coli strains [6] , [7] . (plos.org)
  • On the basis of specific virulence factors and pathogenicity processes, pathogenic E. coli have been subdivided into different pathotypes, including intestinal and extraintestinal strains [1] . (plos.org)
  • EAEC strains were widely distributed across four major E. coli phylogroups. (asm.org)
  • Surveillance of the horizontal transfer of both virulence and ESBL genes among E. coli strains is important for preventing a worldwide increase in antimicrobial drug resistance. (asm.org)
  • The central question of this study was when and how RpoS is stabilized in the two E. coli K-12 laboratory strains W3110 and MC4110. (hu-berlin.de)
  • The microbial mechanisms of certain E. coli strains enhance their pathogenicity, which contribute to the initiation and development of IBD [5] [6] . (kenyon.edu)
  • Though E. coli K12 is a commensal strain, there exist adherent-invasive E. coli (AIEC) strains that are known to contribute to IBD initiation and development. (kenyon.edu)
  • AIEC strains differ from E. coli K12 through physiological mechanisms that alter the pathogenic nature of the bacteria [14] . (kenyon.edu)
  • We found that multidrug-resistant (MDR) phenotypes with enhanced efflux can also be selected by fluoroquinolones from marRAB - or acrAB -inactivated E. coli K-12 strains having a single mutation in the quinolone-resistance-determining region of gyrA . (asm.org)
  • We wondered whether efflux-associated mutations would be selectable in E. coli strains in which regulatory genes or the acrAB locus was inactivated. (asm.org)
  • The AcrA protein of the AcrAB multidrug efflux pump was overexpressed in 19 of 30 strains of E. coli tested, and all 19 strains were tolerant to organic solvents. (asm.org)
  • In Beijing from 1997 to 1999, approximately 60% of Escherichia coli strains isolated from hospital-acquired infections and 50% of the E. coli strains isolated from the community were resistant to ciprofloxacin. (asm.org)
  • Additional strains used in this study were plasmid-free E. coli K-12 derivatives. (asm.org)
  • Growth kinetics of recA , yeiH , asr , srlE , and manY deletion strains compared to wild-type parent E. coli BW25113. (asm.org)
  • Heterogeneous virulence of enteroaggregative Escherichia coli strains isolated from children in southwest Nigeria. (ajtmh.org)
  • Percentage of cyclohexane-tolerant strains among 138 clinical E. coli isolates, grouped according to their ofloxacin MIC. (asmscience.org)
  • As in many other bacterial pathogens, the evolution of E. coli towards pathogenic phenotypes has been determined mainly by two mechanisms: the acquisition of virulence genes and the loss or modification of genes of the core genome [3] . (plos.org)
  • 2015. The RpoE Stress Response Pathway Mediates Reduction of Enteropathogenic Escherichia coli virulence by Zinc . (reed.edu)
  • 2000) The locus of enterocyte effacement (LEE)-encoded regulator controls expression of both LEE- and non-LEE-encoded virulence factors in enteropathogenic and enterohemorrhagic Escherichia coli . (reed.edu)
  • Research into EPEC is intense and provides a good virulence model of other E. coli infections as well as other pathogenic bacteria. (asm.org)
  • Expression of virulence-associated determinants is strictly and coordinately controlled by a set of transcriptional regulators and complex signal transduction cascades are responsible for sensing and integrating the environmental information into the virulence regulons [ 1 - 4 ]. (prolekare.cz)
  • Bansal T, Jesudhasan P, Pillai S, Wood TK, Jayaraman A (2008) Temporal regulation of enterohemorrhagic Escherichia coli virulence mediated by autoinducer-2. (springer.com)
  • Induction of the soxR regulon also leads to diminished levels of the major outer membrane protein OmpF and alteration of the small-subunit ribosomal protein S6. (pnas.org)
  • This protein is a positive regulator for the phosphate regulon. (uniprot.org)
  • Expression of many of the genes in the SOS regulon are controlled by the LexA protein. (nih.gov)
  • It is now appreciated that both S . Typhimurium and E. coli maintain an acidic cytoplasm through the actions of the outer membrane protein regulator OmpR via its regulation of distinct signaling pathways. (frontiersin.org)
  • The second, designated enn5.8193, expresses an IgG-binding protein when cloned in Escherichia coli. (nih.gov)
  • In Escherichia coli, cyclic AMP receptor protein (CRP) can regulate the transcription of more than 100 genes. (wikipedia.org)
  • The pyruvate dehydrogenase regulator protein (PdhR) of Escherichia coli acts as a transcriptional regulator in a pyruvate dependent manner to control central metabolic fluxes. (biomedcentral.com)
  • 2011. Ler of Pathogenic Escherichia coli forms toroidal protein-DNA complexe s. 2011. (reed.edu)
  • The Escherichia coli Fur protein, with its iron(II) cofactor, represses iron assimilation and manganese superoxide dismutase (MnSOD) genes, thus coupling iron metabolism to protection against oxygen toxicity. (semanticscholar.org)
  • Chen S, Vincent S, Wilson DB, Ganem B (2003) Mapping of chorismate mutase and prephenate dehydrogenase domains in the Escherichia coli T-protein. (springer.com)
  • Using the Escherichia coli RR PhoB as a model system, we were able to observe phosphorylation-dependent FRET between fluorescent protein (FP)-PhoB proteins and validated the FRET method by determining dimerization affinity and dimerization-coupled phosphorylation kinetics that recapitulated values determined by alternative methods. (pubmedcentralcanada.ca)
  • The YaaA protein of the Escherichia coli OxyR regulon lessens hydrogen peroxide toxicity by diminishing the amount of intracellular unincorporated iron. (ebi.ac.uk)
  • RT-like protein in E.coli C is larger than RT-like protein in E.coli K-12. (nii.ac.jp)
  • Publications] Kim,S-K.: 'Mutational analysis of the first helix of region 4.2 of the σ70 subunit of Escherichia coli RNA polymerase in transcriptional activation by activator protein PhoB' Mol.Gen.Gent. (nii.ac.jp)
  • Publications] Makino, K.: 'Role of the sigma70 subunit of RNA polymerase in the transcriptional activation by activator protein PhoB in Escherichia coli' Genes & Dev.7. (nii.ac.jp)
  • Colibri provides a complete dataset of DNA and protein sequences derived from the paradigm strain E. coli K-12, linked to the relevant annotations and functional assignments. (davidson.edu)
  • This allows the CpxP protein to be free resulting in binding to CpxA and shutting the Cpx regulon off. (igem.org)
  • The Cpx regulon is known to be activated common agents that increase envelope stress response such as high pH which causes dissolving of membrane and ethanol which is an established protein denaturant. (igem.org)
  • CpxP is a member of the Cpx regulon which docks on to CpxA, a transmembrane protein normally. (igem.org)
  • In E. coli , SulA, RcsA, RpoH and TraJ as well as RNase R, have been identified as natural protein substrates of ClpYQ. (mcponline.org)
  • The ATP-dependent protease of E. coli provides us an excellent example to understand the importance of protein degradation role in cell physiology. (mcponline.org)
  • The LexA protein represses the SOS regulon, which regulates the genes involved in DNA repair. (qmul.ac.uk)
  • At the heart of the regulon is an entirely novel mode of regulation by CRP, in which the competence-specific inducer protein Sxy directs CRP to activate transcription at a new class of CRP sites. (ubc.ca)
  • The cAMP response protein (CRP) is a transcription factor known to regulate many genes in Escherichia coli. (caltech.edu)
  • AidB is a multifunctional protein that forms part of E.coli's adaptive response to alkylating agents as defined by the ada regulon. (unam.mx)
  • Subclones from the soxR region in the delta soxR strain simultaneously restored cellular resistance to the redox-cycling agent phenazine methosulfate and inducibility of at least two of the regulon proteins, glucose-6-phosphate dehydrogenase and endonuclease IV, by paraquat, another redox-cycling agent. (nih.gov)
  • E. coli isolates were selected based on their non-susceptibility to at least one of the carbapenem and E. coli ATCC 25922 was used as the quality control strain. (biomedcentral.com)
  • 22 new genes contained in two operons controlled by PdhR (previously only 20 regulatory targets in eight operons were known) were identified by analysing a large-scale dataset of E. coli from the Many Microbes Microarray Database and novel expression data from a pdhR knockout strain, as well as a PdhR overproducing strain. (biomedcentral.com)
  • A wealth of new information has become available recently for the annotation of Escherichia coli K-12 strain MG1655. (wisc.edu)
  • An example of bacteria that are common to the gut normal flora include the E. coli K 12 strain. (kenyon.edu)
  • Furthermore, the orientation of type 1 pilus of AIEC differs from the E. coli K12 strain and is regulated by various mechanisms. (kenyon.edu)
  • First report of an E. coli strain from swine carrying an OXA-181-carbapenemase and colistin resistance determinant MCR-1. (uni-giessen.de)
  • E. coli K-12 strain AG100 has been previously described ( 13 ). (asm.org)
  • isolated another E. coli mutant strain, strain LH530, that was hypersensitive to hydrophobic and large hydrophilic antibiotics and showed temperature-sensitive growth. (genetics.org)
  • This transport system, named MetP according to its E. coli counterpart was characterized in detail in the metNI deletion strain. (uni-koeln.de)
  • Here, we use a novel small RNA (sRNA) screening approach to identify genes whose knockdown increases ciprofloxacin (CIP) sensitivity in a resistant strain of Escherichia coli . (g3journal.org)
  • Previously, SlyA has been shown to directly regulate only two operons in Escherichia coli K-12 MG1655, fimB and hlyE ( clyA ). (microbiologyresearch.org)
  • In bacterial genomes, the compactly encoded genes and operons are well organized, with genes in the same biological pathway or operons in the same regulon close to each other on the genome sequence. (biomedcentral.com)
  • The results showed that arrangements of operons in the E. coli genome tend to minimize the spatial distance between operons in the same regulon. (biomedcentral.com)
  • However, numerous large regulons exist comprising multiple clustered operons that are separated distantly on the genome sequence. (biomedcentral.com)
  • We extended the PdhR regulon by 22 new genes contained in two operons and validated the regulation of the glcDEFGBA operon for glycolate utilisation and the dcw -cluster for cell division proteins experimentally. (biomedcentral.com)
  • 2000. Activation of the enteropathogenic E. coli (EPEC) LEE2 and LEE3 operons by Ler . (reed.edu)
  • 173, 2864-2871), indicate that SoxR and SoxS may constitute a novel type of two-component regulatory system in which the two proteins act sequentially to activate transcription of the various regulon genes in response to superoxide stress. (nih.gov)
  • Herein, we classify distinct OmpR regulons and also identify shared OmpR regulatory pathways between S . Typhimurium and E. coli in response to acid and osmotic stress. (frontiersin.org)
  • However, recent genome-scale studies have begun to uncover and expand some of the intricate regulatory complexities that underpin the adaptive transcriptional response to nitrogen starvation in E. coli . (biochemsoctrans.org)
  • This is accompanied by a major reorganization of the CRP regulon, which involves a multitude of regulatory RNAs. (prolekare.cz)
  • The regulatory region of the uxuAB operon in Escherichia coli K12. (bireme.br)
  • In this study, we sought to determine whether the regulatory genes ( marA, soxS, acrR ), in addition to the structural genes ( gyrA/B, parC ), contained mutations which contribute to the high-level fluoroquinolone resistance of clinical E. coli isolates from China. (asm.org)
  • The regulatory regions for 119 Escherichia coli promoters have been analyzed, and the locations of the regulatory sites have been cataloged. (asm.org)
  • Additionally, we are working in collaboration with Dr. Julio Collado-Vides lab to develop analysis tools which can be used to map global expression data from E. coli to information such as: operon organization, promoter and regulatory signals upstream of individual genes, and comparisons of the set of affected genes with known and putative transcriptional factors in E.coli. (davidson.edu)
  • However, the homologs of Mac1 and the transcriptional regulatory mechanism of the copper regulon in other fungi have not yet been identified. (biochemj.org)
  • The PhoPQ two-component system acts as a transcriptional regulator that responds to Mg(2+) starvation both in Escherichia coli and Salmonella typhimurium (Garcia et al. (nih.gov)
  • Experimental validation of predicted targets for the well-studied fumarate nitrate reductase regulator showed the effectiveness of our approach in Escherichia coli . (biomedcentral.com)
  • The dual transcriptional regulator CysR in Corynebacterium glutamicum ATCC 13032 controls a subset of genes of the McbR regulon in response to the availability of sulphide acceptor molecules. (sigmaaldrich.com)
  • CysR acts as the functional analogue of the unrelated LysR-type regulator CysB from Escherichia coli, controlling sulphide production in response to acceptor availability. (sigmaaldrich.com)
  • Experimentally, the production of the active LasR quorum-sensing regulator was studied in an Escherichia coli background as a function of signal molecule concentration. (sigmaaldrich.com)
  • Comparison of the PhoPQ regulon in Escherichia coli and Salmonella typhimurium. (nih.gov)
  • Oxidative stress responses in Escherichia coli and Salmonella typhimurium. (asm.org)
  • Recent progress on the genetics and molecular biology of the cellular responses to oxidative stress, primarily in Escherichia coli and Salmonella typhimurium, is summarized. (asm.org)
  • In recent years, a great deal of interest focused on chromosomal multidrug resistance such as the multiple antibiotic resistance ( mar ) locus in Escherichia coli and Salmonella spp. (asmscience.org)
  • Ahmer BM (2004) Cell-to-cell signalling in Escherichia coli and Salmonella enterica. (springer.com)
  • abstract = "Escherichia coli K-12 can readily mutate to use methylphosphonic acid as the sole phosphorus source by a direct carbon-to-phorphorus (C-P) bond cleavage activity that releases methane and P(i). (umn.edu)
  • AggR is a member of the AraC/XylS family of bacterial transcriptional activators ( 10 , 11 ), exhibiting the greatest levels of amino acid identity with the CfaD (68%), Rns (66%), and CsvR (62%) regulators of enterotoxigenic E. coli (ETEC) ( 11 ). (asm.org)
  • Many bacterial pathogens experience nitrogen limitation in host environments such as the urinary tract (e.g. uropathogenic Escherichia coli [ 1 ]) or macrophages (e.g. (biochemsoctrans.org)
  • The DNA interaction data of Escherichia coli , measured by the genome conformation capture (GCC) technique, have recently become available, which allowed us to investigate the spatial features of bacterial genome organization. (biomedcentral.com)
  • Pathogenic Escherichia coli is a known bacterial cause of IBD. (kenyon.edu)
  • Highly diverse and antimicrobial susceptible Escherichia coli display a naïve bacterial population in fruit bats from the Republic of Congo. (uni-giessen.de)
  • Aizenman E, Engelberg-Kulka H, Glaser G (1996) An Escherichia coli chromosomal "addiction module" regulated by guanosine [corrected] 3′,5′-bispyrophosphate: a model for programmed bacterial cell death. (springer.com)
  • Enteroaggregative Escherichia coli (EAEC) is increasingly recognized as a cause of diarrhea in adults and children in both industrialized and nonindustrialized countries ( 1 - 5 ). (asm.org)
  • Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea. (asm.org)
  • Enteroaggregative Escherichia coli (EAEC) causes acute or persistent diarrhea in children and adults, in both developing ( 1 ) and industrialized ( 2 , 3 ) countries. (asm.org)
  • Enteroaggregative Escherichia coli (EAEC) pathogenesis is thought to comprise intestinal colonization followed by the release of enterotoxins and cytotoxins. (ajtmh.org)
  • Enteroaggregative Escherichia coli as a cause of traveler's diarrhea: clinical response to ciprofloxacin. (ajtmh.org)
  • Enteroaggregative Escherichia coli as a major etiologic agent in traveler's diarrhea in 3 regions of the world. (ajtmh.org)
  • Successful treatment of diarrheal disease associated with enteroaggregative Escherichia coli in adults infected with human immunodeficiency virus. (ajtmh.org)
  • Enteroaggregative Escherichia coli as a potential cause of diarrheal disease in adults infected with human immunodeficiency virus. (ajtmh.org)
  • Intestinal infection due to enteroaggregative Escherichia coli among human immunodeficiency virus-infected persons. (ajtmh.org)
  • We have constructed operon fusions of the E. coli lac genes to the soxS promoter to monitor soxS transcription. (asm.org)
  • Mutations that progressively changed the periplasmic nitrate sensing region, the P box, of E. coli NarQ to the sequence of the corresponding region of gonococcal NarQ resulted in loss of transcription activation in response to the availability of either nitrate or nitrite. (biomedcentral.com)
  • Despite the sequence similarities between transcription activators of E. coli and N. gonorrhoeae , these results emphasise the fundamental differences in transcription regulation between these two types of pathogenic bacteria. (biomedcentral.com)
  • The adaptive response to N starvation in Escherichia coli is primarily a 'scavenging response', which results in the transcription of genes required for the transport and catabolism of nitrogenous compounds. (biochemsoctrans.org)
  • In contrast with the major E. coli σ 70 family of σ factors involved in adaptive responses to diverse stress cues, including housekeeping functions, σ 54 directs its RNAP (Eσ 54 ) to promoters uniquely characterised by consensus dinucleotide sequences positioned at −24 and −12 relative to the transcription start site at +1. (biochemsoctrans.org)
  • In Escherichia coli, SoxS, MarA and Rob form a closely related subset of the AraC/XylS family of positive regulators, sharing approximately 42% amino acid sequence identity over the length of SoxS and the ability to activate transcription of a common set of target genes that provide resistance to redox-cycling compounds and antibiotics. (umass.edu)
  • We show that expression of TetD activates transcription of a subset of the SoxS/MarA/Rob regulon genes and confers resistance to redox-cycling compounds and antibiotics. (umass.edu)
  • Further, we show that WhiB does not act independently but rather coregulates its regulon of sporulation genes with a partner transcription factor, WhiA. (asm.org)
  • The Escherichia coli proU promoter element and its contribution to osmotically signaled transcription activation. (reed.edu)
  • 2001) where all necessary components needed for in vitro transcription and translation are purified from E. coli . (neb.com)
  • This system functions through the transcription factors, Pho4 and Pho2, which control the expression of phosphate metabolism related genes called the PHO regulon. (elifesciences.org)
  • Yeast ( Saccharomyces cerevisiae ) PHO regulon is mainly controlled by the phosphorylation and dephosphorylation of the transcription factor PHO4. (plantphysiol.org)
  • When copper is limited in cells, copper regulons are activated by the metallo-transcription factor Mac1 [ 14 - 16 ]. (biochemj.org)
  • We have recently identified the components of the H. influenzae competence regulon (25 genes in 13 transcription units). (ubc.ca)
  • Here we provide additional characterization of the AggR regulon. (asm.org)
  • Characterization of TetD as a transcriptional activator of a subset of genes of the Escherichia coli SoxS/MarA/Rob regulon. (umass.edu)
  • Characterization of the ferrous iron uptake system of Escherichia coli. (semanticscholar.org)
  • Identification and characterization of new iron-regulated Escherichia coli genes by a fur titration assay. (semanticscholar.org)
  • Expression of a key group of these inducible proteins is controlled at the transcriptional level by the soxRS locus (the soxRS regulon). (asm.org)
  • Such mutations lead to overexpression of MarA, a transcriptional activator negatively regulated by MarR ( 9 , 28 ), or of SoxS, the activator of the superoxide SoxRS regulon ( 1 , 28 ), and thereby confer increased resistance to chemically unrelated antibiotics by activating or depressing a number of genetic loci in E. coli that contribute in a synergistic way to the multiple-antibiotic resistance or multidrug resistance (MDR) phenotype. (asm.org)
  • these genes constitute the OxyR and SoxRS regulons. (asm.org)
  • Positive control of a global antioxidant defense regulon activated by superoxide-generating agents in Escherichia coli. (pnas.org)
  • The soxR locus of Escherichia coli K12 mediates transcriptional activation of a complex oxidative stress regulon in response to superoxide-generating (redox-cycling) agents. (nih.gov)
  • Control of Escherichia coli superoxide dismutase (sodA and sodB) genes by the ferric uptake regulation (fur) locus. (semanticscholar.org)
  • In the bacterium Escherichia coli , chromosome replication initiates bidirectionally from a single locus, oriC , and terminates at the diametrically opposed ter region ( 1 ). (asm.org)
  • The mar locus was discovered when the genetic basis of tetracycline resistance in E. coli was investigated. (asmscience.org)
  • Lethal oxidative damage and mutagenesis are generated by iron in delta fur mutants of Escherichia coli: protective role of superoxide dismutase. (semanticscholar.org)
  • We demonstrate that several regulons involved in oxidative stress, osmotic stress, and resistance to antimicrobial and toxic compounds were upregulated in the lysates. (usda.gov)
  • Befitting the hypothesis that EcO157 cells need to adapt to stressful physicochemical conditions in damaged lettuce tissue, we observed the upregulation of multiple genes associated with osmotic stress and oxidative stress, of genes belonging to the Mar antibiotic resistance regulon, and of others involved in detoxification of noxious compounds. (usda.gov)
  • Regulation of MalT and its regulon. (hindawi.com)
  • Regulation of the SOS response regulon in E. coli. (coursehero.com)
  • 2007. SOS regulation of the type III secretion system of enteropathogenic Escherichia coli . (reed.edu)
  • Response to oxygen availability and evidence for growth phase regulation in E. coli . (reed.edu)
  • The schemes of regulation of the two regulons by their respective regulators are reviewed in detail, and the overlaps of these regulons with other stress responses such as the heat shock and SOS responses are discussed. (asm.org)
  • Control site location and transcriptional regulation in Escherichia coli. (asm.org)
  • Clarke MB, Sperandio V (2005) Transcriptional regulation of flhDC by QseBC and sigma (FliA) in enterohaemorrhagic Escherichia coli . (springer.com)
  • The evolution of TyrA proteins within the broader context of how their regulation evolved and to what extent TyrA co-evolved with other genes as common members of aromatic-pathway regulons is now feasible as an emerging topic of ongoing inquiry. (biomedcentral.com)
  • Identification of additional genes belonging to the LexA regulon in Escherichia coli ," Molecular Microbiology , vol. 35, no. 6, pp. 1560-1572, 2000. (hindawi.com)
  • E. coli K12 are commensals that play a role in resisting the colonization of pathogenic bacteria [13] . (kenyon.edu)
  • Although considered a nonpathogenic commensal present in feces, in developing countries pathogenic E. coli is a major cause of intestinal infections associated with high childhood mortality and morbidity. (asmscience.org)
  • Croxen MA, Law RJ, Scholz R, Keeney KM, Wlodarska M, Finlay BB (2013) Recent advances in understanding enteric pathogenic Escherichia coli . (springer.com)
  • Members of the soxR regulon include at least three proteins with demonstrable antioxidant roles: Mn-containing superoxide dismutase (which destroys superoxide radicals), endonuclease IV (which repairs radical-induced damages in DNA), and glucose-6-phosphate dehydrogenase (which produces NADPH). (pnas.org)
  • Mapping of two ugp genes coding for the pho regulon-dependent sn-glycerol-3-phosphate transport system of Escherichia coli. (cngb.org)
  • The in vivo C-P lyase activity is both physiologically and genetically regulated as a member of the phosphate regulon. (umn.edu)
  • SoxS is evidently the proximal activator of the regulon genes: antibiotic resistance and high-level expression of at least three of the regulon proteins was effected in vivo by the individual expression of SoxS, but not of SoxR, whether or not the cells were exposed to paraquat. (nih.gov)
  • A total of 198 consecutive, non-duplicates, Escherichia coli isolates were selected for the study. (biomedcentral.com)
  • To analyse the expressional level of efflux pump genes acrA and acrB in multidrug resistant clinical isolates of Escherichia coli quantitative Real Time PCR was performed. (biomedcentral.com)
  • The genetic basis for fluoroquinolone resistance was examined in 30 high-level fluoroquinolone-resistant Escherichia coli clinical isolates from Beijing, China. (asm.org)
  • How does a native E. coli cell combat periplasmic stress? (igem.org)
  • Binds to a 16-bp palindromic sequence located within the promoter region of pur regulon genes. (proteopedia.org)
  • The availability of the complete genome sequence and pan- Neisseria microarrays provide an opportunity to test these assumptions directly by comparing the extent of the N. gonorrhoeae FNR regulon with that of the recently-published E. coli FNR regulon [ 8 ]. (biomedcentral.com)
  • However, the genome-wide DNA binding properties of SeqA are unknown, and hence, here, we describe a study of the binding of SeqA across the entire Escherichia coli K-12 chromosome, using chromatin immunoprecipitation in combination with DNA microarrays. (asm.org)
  • EcoGene is a collection of information about the genes, proteins, and intergenic regions of the E. coli K-12 genome and proteome accumulated during years of sequence analysis and literature surveys by Kenn Rudd and his collaborators: Mary Berlyn of the E. coli Genetic Stock Center, Amos Bairoch of SWISS-PROT, and Antoine Danchin and Ivan Mozser of Colibri. (davidson.edu)
  • E coli have several native sigma factors in the genome that are upregulated during envelope stress. (igem.org)
  • High-level fluoroquinolone resistance in Escherichia coli is associated with mutations in the genes coding for the target proteins DNA gyrase ( gyrA ) and topoisomerase IV ( parC ) ( 3 , 33 , 39 ). (asm.org)
  • Knockout experiments have shown that the AcrAB pump is critical for the resistance of E. coli to bile salts ( 40 ) and a number of antibiotics and that mar mutations are effective only in the presence of this pump ( 32 ). (asm.org)
  • Upon deletion or inactivation of AcrAB, E. coli cells without topoisomerase mutations become hypersusceptible and resistance to fluoroquinolones can be reversed in topoisomerase mutants ( 31 ). (asm.org)
  • The decarboxylation of glutamate to 4-aminobutanoate, also known as gamma-aminobutyrate or GABA, coupled with glutamate-GABA antiport, provides a robust mechanism of glutamate-based acid resistance for Escherichia coli and other organisms that need to survive transient exposures to highly acidic pH. (jcvi.org)
  • Castanie-Cornet MP, Penfound TA, Smith D, Elliott JF, Foster JW (1999) Control of acid resistance in Escherichia coli . (springer.com)
  • By directed evolution in the laboratory, we previously generated populations of Escherichia coli that exhibit a complex new phenotype, extreme resistance to ionizing radiation (IR). (elifesciences.org)
  • The experiments were performed on populations of E. coli cells that had been subjected to an evolutionary selection for extremely high resistance to ionizing radiation. (elifesciences.org)
  • The soxR regulon may thus operate as an inducible defense against xenobiotics in general. (pnas.org)
  • DNA sequence analysis revealed the presence of two genes involved in activating the soxR regulon. (nih.gov)
  • In this study, we show that the E . coli ompR malT con double mutant exhibits a synthetic lethal phenotype that is environmentally conditional. (hindawi.com)
  • Cohen GN, Adelberg EA (1958) Kinetics of incorporation of p-fluorophenylalanine by a mutant of Escherichia coli resistant to this analogue. (springer.com)
  • A) About 30 genes around the E. coli chromosome are normally repressed by the binding of a LexA dimer (barbell structure) to their operators. (coursehero.com)
  • In this study, we describe how we have been able to identify scores of targets, across the entire Escherichia coli chromosome, to which SeqA binds. (asm.org)
  • There are 19,120 GATC motifs scattered throughout the E. coli K-12 chromosome, and these sites may also serve as binding targets for SeqA. (asm.org)
  • We've also found that this regulon is ancestral in many related pathogens including Escherichia coli and Vibrio cholerae, suggesting that many more bacteria may be naturally competent than previously thought. (ubc.ca)
  • Escherichia coli responds to superoxide-generating agents by inducing approximately 40 proteins. (pnas.org)
  • Escherichia coli responds to the redox stress imposed by superoxide-generating agents such as paraquat by activating the synthesis of as many as 80 polypeptides. (asm.org)
  • However, a comprehensive comparison of OmpR regulons between S . Typhimurium and E. coli is lacking. (frontiersin.org)
  • Further application of the FRET method to all E. coli OmpR/PhoB subfamily RRs revealed that phosphorylation-activated RR interaction is indeed a common theme for OmpR/PhoB subfamily RRs and these RRs display significant interaction specificity. (pubmedcentralcanada.ca)
  • In Escherichia coli , the PHO regulon comprises at least 15 genes involved in the acquisition of Pi ( Torriani, 1990 ). (plantphysiol.org)
  • SUMMARY Enteropathogenic Escherichia coli (EPEC) remains an important cause of diarrheal disease worldwide. (asm.org)
  • 2012. Zinc-induced envelope stress diminishes type III secretion in enteropathogenic Escherichia coli . (reed.edu)
  • Diarrheal illness is a major public health problem worldwide, with over 2 million deaths occurring each year, particularly among infants younger than 5 years ( www.who.int ). One of the most common causes of infantile diarrhea is enteropathogenic Escherichia coli (EPEC). (asm.org)
  • Mutagenesis and stress responses induced in Escherichia coli by hydrogen peroxide. (semanticscholar.org)
  • To verify this hypothesis, we compared the composition of the PhoPQ regulon between E. coli and S. typhimurium using a combination of expression experiments and motif data. (nih.gov)
  • This allowed us to reconstruct the direct PhoPQ-dependent regulons in E. coli K12 and S. typhimurium LT2. (nih.gov)
  • Comparison of both regulons revealed a very limited overlap of PhoPQ-dependent genes between both organisms. (nih.gov)
  • This study revealed the organization features of Escherichia coli genomic functional units in the 3D space and furthered our understanding of the link between the three-dimensional structure of chromosomes and biological function. (biomedcentral.com)
  • During the transition from its innocuous E. coli ancestor, Shigella , the aetiological agent of bacillary dysentery, has undergone drastic genomic rearrangements affecting the polyamine profile. (plos.org)
  • Finally, we are working with other groups to correlate and reconcile the various lists and databases containing E. coli genomic information (see, for example, the sites listed at the E. coli Database Portal ). (wisc.edu)
  • Its purpose is to collate and integrate various aspects of the genomic information from E. coli , the paradigm of Gram-negative bacteria. (davidson.edu)
  • Natural history of enteroaggregative and enterotoxigenic Escherichia coli infection among US travelers to Guadalajara, Mexico. (ajtmh.org)
  • There are two metabolic pathways for NAD + biosynthesis in Escherichia coli , the de novo pathway and the salvage pathway ( 3 , 15 ). (asm.org)
  • MetaCy c contains the E. coli pathways of EcoCyc, plus additional pathways that have been gathered from a variety of literature and on-line sources, with citations to the source of each pathway. (davidson.edu)
  • Figure illustrates Cpx regulon activation pathway. (igem.org)
  • This review summarises the transcriptional basis underpinning how E. coli adapts to N starvation, specifically highlighting two new branches in this important adaptive process. (biochemsoctrans.org)
  • Together, these results show that nitrogen starvation causes intense changes in the X. fastidiosa transcriptome and some of these differentially expressed genes belong to the σ 54 regulon. (biomedcentral.com)
  • Looking into the Gap between Commensal E. coli and Harmful Shigella . (plos.org)
  • Escherichia coli is not only a harmless commensal of the human and animal intestine, but also a major cause of morbidity and mortality [1] , [2] . (plos.org)
  • 3. Tschantz, W.R., Sung, M., Delgado-Partin, V.M. & Dalbey, R.E. (1993) A serine and a lysine residue implicated in the catalytic mechanism of the Escherichia coli leader peptidase. (qmul.ac.uk)
  • 1jhz is a 2 chain structure with sequence from "bacillus_coli"_migula_1895 "bacillus coli" migula 1895 . (proteopedia.org)
  • The cell envelope of Gram-negative bacteria such as Escherichia coli consists of an inner membrane (IM), an aqueous peptidoglycan-containing periplasm, and an outer membrane (OM) ( 1 ). (pnas.org)
  • The gal operon of E. coli consists of 4 structural genes: galE (epimerase), galT (galactose transferase), galK (galactokinase), and galM (mutarotase) which are transcribed from two overlapping promoters, PG1 (+1) and PG2 (-5), upstream from galE. (wikipedia.org)
  • Two different Escherichia coli proP promoters respond to osmotic and growth phase signals. (reed.edu)
  • A more complete picture of the σ 54 regulon was achieved by combining the transcriptome data with an in silico search for potential σ 54 -dependent promoters, using a position weight matrix approach. (biomedcentral.com)
  • The Escherichia coli gal operon contains two operators, O E and O I , which flank two overlapping promoters located in between. (els.net)
  • sequenced the genomes of the E. coli after 20, 40 or 50 rounds of the selection process, and compared mutations found in the four separate evolved populations. (elifesciences.org)
  • The nucleotide sequence of the glpEGR operon of Escherichia coli was determined. (asm.org)
  • Structures of Escherichia coli DNA adenine methyltransferase (Dam) in complex with a non-GATC sequence: potential implications for methylation-independent transcriptional repression. (expasy.org)
  • 1dbq is a 2 chain structure with sequence from Escherichia coli . (proteopedia.org)
  • These experiments have allowed us to identify seven new LexA-regulated genes, thus bringing the present number of genes in the E. coli LexA regulon to 31. (nih.gov)