beta-Galactosidase: A group of enzymes that catalyzes the hydrolysis of terminal, non-reducing beta-D-galactose residues in beta-galactosides. Deficiency of beta-Galactosidase A1 may cause GANGLIOSIDOSIS, GM1.DNA Polymerase III: A DNA-dependent DNA polymerase characterized in E. coli and other lower organisms but may be present in higher organisms. Use also for a more complex form of DNA polymerase III designated as DNA polymerase III* or pol III* which is 15 times more active biologically than DNA polymerase I in the synthesis of DNA. This polymerase has both 3'-5' and 5'-3' exonuclease activities, is inhibited by sulfhydryl reagents, and has the same template-primer dependence as pol II. EC 2.7.7.7.Galactosidases: A family of galactoside hydrolases that hydrolyze compounds with an O-galactosyl linkage. EC 3.2.1.-.alpha-Galactosidase: An enzyme that catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-galactosides including galactose oligosaccharides, galactomannans, and galactolipids.Complement C3c: A 206-amino-acid fragment in the alpha chain (672-1663) of C3b. It is generated when C3b is inactivated (iC3b) and its alpha chain is cleaved by COMPLEMENT FACTOR I into C3c (749-954), and C3dg (955-1303) in the presence COMPLEMENT FACTOR H.Cerebrosides: Neutral glycosphingolipids that contain a monosaccharide, normally glucose or galactose, in 1-ortho-beta-glycosidic linkage with the primary alcohol of an N-acyl sphingoid (ceramide). In plants the monosaccharide is normally glucose and the sphingoid usually phytosphingosine. In animals, the monosaccharide is usually galactose, though this may vary with the tissue and the sphingoid is usually sphingosine or dihydrosphingosine. (From Oxford Dictionary of Biochemistry and Molecular Biology, 1st ed)Lactose: A disaccharide of GLUCOSE and GALACTOSE in human and cow milk. It is used in pharmacy for tablets, in medicine as a nutrient, and in industry.Galactose: An aldohexose that occurs naturally in the D-form in lactose, cerebrosides, gangliosides, and mucoproteins. Deficiency of galactosyl-1-phosphate uridyltransferase (GALACTOSE-1-PHOSPHATE URIDYL-TRANSFERASE DEFICIENCY DISEASE) causes an error in galactose metabolism called GALACTOSEMIA, resulting in elevations of galactose in the blood.Acridine Orange: A cationic cytochemical stain specific for cell nuclei, especially DNA. It is used as a supravital stain and in fluorescence cytochemistry. It may cause mutations in microorganisms.Escherichia coli: A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.alpha-L-Fucosidase: An enzyme that catalyzes the hydrolysis of an alpha L-fucoside to yield an alcohol and L-fucose. Deficiency of this enzyme can cause FUCOSIDOSIS. EC 3.2.1.51.Fabry Disease: An X-linked inherited metabolic disease caused by a deficiency of lysosomal ALPHA-GALACTOSIDASE A. It is characterized by intralysosomal accumulation of globotriaosylceramide and other GLYCOSPHINGOLIPIDS in blood vessels throughout the body leading to multi-system complications including renal, cardiac, cerebrovascular, and skin disorders.Escherichia coli Proteins: Proteins obtained from ESCHERICHIA COLI.Nigericin: A polyether antibiotic which affects ion transport and ATPase activity in mitochondria. It is produced by Streptomyces hygroscopicus. (From Merck Index, 11th ed)Glycoside HydrolasesGlycosides: Any compound that contains a constituent sugar, in which the hydroxyl group attached to the first carbon is substituted by an alcoholic, phenolic, or other group. They are named specifically for the sugar contained, such as glucoside (glucose), pentoside (pentose), fructoside (fructose), etc. Upon hydrolysis, a sugar and nonsugar component (aglycone) are formed. (From Dorland, 28th ed; From Miall's Dictionary of Chemistry, 5th ed)Cell Aging: The decrease in the cell's ability to proliferate with the passing of time. Each cell is programmed for a certain number of cell divisions and at the end of that time proliferation halts. The cell enters a quiescent state after which it experiences CELL DEATH via the process of APOPTOSIS.Escherichia coli Infections: Infections with bacteria of the species ESCHERICHIA COLI.Histocytochemistry: Study of intracellular distribution of chemicals, reaction sites, enzymes, etc., by means of staining reactions, radioactive isotope uptake, selective metal distribution in electron microscopy, or other methods.Interleukin-1beta: An interleukin-1 subtype that is synthesized as an inactive membrane-bound pro-protein. Proteolytic processing of the precursor form by CASPASE 1 results in release of the active form of interleukin-1beta from the membrane.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Escherichia coli O157: A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.beta 2-Microglobulin: An 11-kDa protein associated with the outer membrane of many cells including lymphocytes. It is the small subunit of the MHC class I molecule. Association with beta 2-microglobulin is generally required for the transport of class I heavy chains from the endoplasmic reticulum to the cell surface. Beta 2-microglobulin is present in small amounts in serum, csf, and urine of normal people, and to a much greater degree in the urine and plasma of patients with tubular proteinemia, renal failure, or kidney transplants.Base Sequence: The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.Genes, Bacterial: The functional hereditary units of BACTERIA.Bacterial Proteins: Proteins found in any species of bacterium.Receptors, Adrenergic, beta: One of two major pharmacologically defined classes of adrenergic receptors. The beta adrenergic receptors play an important role in regulating CARDIAC MUSCLE contraction, SMOOTH MUSCLE relaxation, and GLYCOGENOLYSIS.Integrin beta3: An integrin beta subunit of approximately 85-kDa in size which has been found in INTEGRIN ALPHAIIB-containing and INTEGRIN ALPHAV-containing heterodimers. Integrin beta3 occurs as three alternatively spliced isoforms, designated beta3A-C.Escherichia coli K12: A species of gram-negative, rod-shaped bacteria belonging to the K serogroup of ESCHERICHIA COLI. It lives as a harmless inhabitant of the human LARGE INTESTINE and is widely used in medical and GENETIC RESEARCH.Cloning, Molecular: The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.Plasmids: Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Mutation: Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.DNA, Bacterial: Deoxyribonucleic acid that makes up the genetic material of bacteria.Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Genetic Complementation Test: A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.Models, Molecular: Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.Protein Conformation: The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).X-Ray Diffraction: The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Fatigue: The state of weariness following a period of exertion, mental or physical, characterized by a decreased capacity for work and reduced efficiency to respond to stimuli.Databases, Protein: Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.Sequence Analysis, Protein: A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.Systems Integration: The procedures involved in combining separately developed modules, components, or subsystems so that they work together as a complete system. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Proteome: The protein complement of an organism coded for by its genome.Proteins: Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.Immunoglobulin Fab Fragments: Univalent antigen-binding fragments composed of one entire IMMUNOGLOBULIN LIGHT CHAIN and the amino terminal end of one of the IMMUNOGLOBULIN HEAVY CHAINS from the hinge region, linked to each other by disulfide bonds. Fab contains the IMMUNOGLOBULIN VARIABLE REGIONS, which are part of the antigen-binding site, and the first IMMUNOGLOBULIN CONSTANT REGIONS. This fragment can be obtained by digestion of immunoglobulins with the proteolytic enzyme PAPAIN.Recombinant Proteins: Proteins prepared by recombinant DNA technology.rab GTP-Binding Proteins: A large family of MONOMERIC GTP-BINDING PROTEINS that play a key role in cellular secretory and endocytic pathways. EC 3.6.1.-.rab5 GTP-Binding Proteins: A genetically related subfamily of RAB GTP-BINDING PROTEINS involved in transport from the cell membrane to early endosomes. This enzyme was formerly listed as EC 3.6.1.47.
Matthews BW, Jacobson RH, Zhang XJ, DuBose RF (1994). "Three-dimensional structure of beta-galactosidase from E. coli". Nature ... beta-galactosidase (EC 3.2.1.23); beta-mannosidase (EC 3.2.1.25); beta-glucuronidase (EC 3.2.1.31). These enzymes contain a ... The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. The sugar ... beta-galactosidase from Escherichia coli". J. Biol. Chem. 267 (16): 11126-11130. PMID 1350782. ...
"A structural view of the action of Escherichia coli (lacZ) beta-galactosidase". Biochemistry. 40 (49): 14781-94. doi:10.1021/ ... The Lactase Protein E. coli β-galactosidase: PDB: 1JYY​ Gene Ontology for Lactase Making of the Fittest: Got Lactase? The Co- ... Studies of E. coli lactase have proposed that hydrolysis is initiated when a glutamate nucleophile on the enzyme attacks from ... Lactase (also known as lactase-phlorizin hydrolase, or LPH), a part of the β-galactosidase family of enzymes, is a glycoside ...
"On the subunit structure of wild-type versus complemented beta-galactosidase of Escherichia coli". Journal of Molecular Biology ... of a peptide fraction of Escherichia coli beta-galactosidase". Journal of Molecular Biology. 12 (3): 918-923. doi:10.1016/S0022 ... of the inducible alleles and the constitutive alleles in the synthesis of beta-galactosidase in zygotes of Escherichia coli". ... of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli". ...
"Affibody-beta-galactosidase immunoconjugates produced as soluble fusion proteins in the Escherichia coli cytosol". J. Immunol. ... "Construction and characterization of affibody-Fc chimeras produced in Escherichia coli". J. Immunol. Methods. 261 (1-2): 199- ...
A common reporter in bacteria is the E. coli lacZ gene, which encodes the protein beta-galactosidase. This enzyme causes ... Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity. ... in the β-galactosidase system. As a result, the reporter gene's expression is independent of the gene of interest's expression ...
... beta lactamase and beta galactosidase). It is often used in conjunction with an ampicillin-susceptible E. coli strain to teach ...
... of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli". ... However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this ... Langley, K. E.; Villarejo, M. R.; Fowler, A. V.; Zamenhof, P. J.; Zabin, I. (1975). "Molecular basis of beta-galactosidase ... β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ...
... beta lactamase, beta galactosidase. The yeast component of a yeast shuttle vector includes an autonomously replicating sequence ... coli and mammals. Shuttle vectors are frequently used to quickly make multiple copies of the gene in E. coli (amplification). ... both Saccharomyces cerevisiae and Escherichia coli) or in different species of bacteria (e.g. both E. coli and Rhodococcus ... The E. coli component of a yeast shuttle vector includes an origin of replication and a selectable marker, e.g. antibiotic ...
... of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli". ... However, a mutant β-galactosidase derived from the M15 strain of E. coli has its N-terminal residues 11-41 deleted and this ... "Molecular basis of beta-galactosidase alpha-complementation". Proceedings of the National Academy of Sciences of the United ... β-galactosidase is a protein encoded by the lacZ gene of the lac operon, and it exists as a homotetramer in its active state. ...
Because E. coli produces both beta-galactosidase and beta-glucuronidase, a combination of two dyes makes it possible to ... when acted on by the enzyme beta-galactosidase, produces a characteristic color. The enzyme beta-galactosidase is a marker for ... Assays typically include a second sugar linked to a different dye which, when acted on by the enzyme beta-glucuronidase, ... Fresno, CA (2009)."E. coli or Fecal Coliform Bacteria Contamination in Your Water Supply." Notice distributed to private well ...
One of the important occurrences of glycoside hydrolases in bacteria is the enzyme beta-galactosidase (LacZ), which is involved ... in regulation of expression of the lac operon in E. coli. In higher organisms glycoside hydrolases are found within the ...
... operon allows for the effective digestion of lactose when glucose is not available through the activity of beta-galactosidase. ... The discovery of cAMP in E. coli led to the demonstration that mutants defective the cya gene but not the crp gene could be ... and is commonly used as a substrate for assay of β-galactosidase in vitro. Colonies that produce β-galactosidase are turned ... "ONPG (β-Galactosidase) test". September 2000. Joung J, Ramm E, Pabo C (2000). "A bacterial two-hybrid selection system for ...
... including the well-studied alpha-galactosidase and beta-galactosidase. Genetically manipulated E. coli bacteria can digest ...
A lactose analog is added to the bacteria, which is then degraded by beta-galactosidase, thereby producing a colored compound ... The E. coli are further modified in order to have a number of mutations including a uvrA mutation which renders the strain ... The degree of color development is an indirect measure of the beta-galactosidase produced, which itself is directly related to ... Taking advantage of an operon fusion placing the lac operon (responsible for producing beta-galactosidase, a protein which ...
Human β-glucuronidase is homologous to the Escherichia coli enzyme β-galactosidase. This homologous relationship, along with ... 1985). "Isolation and expression in Escherichia coli of a cDNA clone encoding human beta-glucuronidase". Gene. 34 (1): 105-10. ... Beta-glucuronidase contains several notable structural formations, including a type of beta barrel known as a jelly roll barrel ... "Entrez Gene: GUSB glucuronidase, beta". Islam MR, Grubb JH, Sly WS (1993). "C-terminal processing of human beta-glucuronidase. ...
Matthews BW (June 2005). "The structure of E. coli beta-galactosidase". C. R. Biol. 328 (6): 549-56. doi:10.1016/j.crvi.2005.03 ... A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase- ... proposed a new isoform for beta-galactosidase with optimum activity at pH 6.0 (Senescence Associated beta-gal or SA-beta-gal) ... "Senescence-associated beta-galactosidase is lysosomal beta-galactosidase". Aging Cell. 5 (2): 187-95. doi:10.1111/j.1474- ...
... such as the gene coding for beta-galactosidase, a hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into ... IPTG uptake by E. coli can be independent of the action of lactose permease, since other transport pathways are also involved. ... permanent dead link] Marbach A, Bettenbrock K (2012). "lac operon induction in Escherichia coli: Systematic comparison of IPTG ... IPTG, unlike allolactose, is not hydrolyzable by β-galactosidase. Therefore, its concentration remains constant during an ...
... glucosides and lactosides but not to the beta-galactosidase which is coded by lacZ in the lac operon of E. coli. The ... galactosidase acetyltransferase of the classical E.coli lac operon is an enzyme whose biological role remain unclear. Its ... the Escherichia coli LacA Gene Product". J. Biol. Chem. 270 (44): 26326-31. doi:10.1074/jbc.270.44.26326. PMID 7592843. Wang, ...
"Molecular cloning and expression in Escherichia coli of a Trichoderma viride endo-β-(1→6)-galactanase gene". Biochem. J. 377 ( ... Galactan endo-1,6-beta-galactosidase (EC 3.2.1.164, endo-1,6-beta-galactanase) is an enzyme with systematic name endo-beta-(1-> ... Galactan endo-1,6-beta-galactosidase at the US National Library of Medicine Medical Subject Headings (MeSH) Molecular and ... beta-galactans to yield galactose and (1->6)-beta-galactobiose as the final products The enzyme specifically hydrolyses 1,6- ...
18 Sep 2007). "Directed evolution of a beta-galactosidase from Pyrococcus woesei resulting in increased thermostable beta- ... "Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: Isolation and some properties of the ...
... it was found that segregational stability of an inserted plasmid expressing beta-galactosidase was increased by between 8 and ... The toxic ToxN protein is approximately 170 amino acids long and has been shown to be toxic to E. coli. The toxic activity of ... An experiment where five TA systems were deleted from a strain of E. coli found no evidence that the TA systems conferred an ... Tsilibaris V, Maenhaut-Michel G, Mine N, Van Melderen L (2007). "What Is the Benefit to Escherichia coli of Having Multiple ...
... beta-galactosidase, SlyD, and others. Typical binding capacities of resins range from 1 to 4 mg/ml resin. Because nanoCLAMPs ... nanoCLAMPs are easily produced in the cytoplasm of E. coli, with typical yields in the range of 50 to 300 mg/L culture. Because ... adjacent loops that connect the beta strands making up the beta-sandwich fold, conferring binding affinity and specificity for ... "Isolation and characterization of a polyol-responsive monoclonal antibody useful for gentle purification of Escherichia coli ...
Jefferson, R. A.; Burgess, S. M.; Hirsh, D (1986). "Beta-Glucuronidase from Escherichia coli as a gene-fusion marker". ... Other competing systems are based on e.g. luciferase, GFP, beta-galactosidase, chloramphenicol acetyltransferase (CAT), ... The technique is based on β-glucuronidase, an enzyme from the bacterium Escherichia coli; this enzyme, when incubated with some ... Jefferson, R. A.; Kavanagh, T. A.; Bevan, M. W. (1987). "GUS fusions: Beta-glucuronidase as a sensitive and versatile gene ...
Organisms meant to produce enzymes such as beta galactosidase, invertase or other amylases may be fed starch to select for ... Examples include single cell protein, bakers yeast, lactobacillus, E. coli, and others. In the case of single-cell protein, ... In the early days of the biotechnology industry, most biopharmaceutical products were made in E. coli; by 2004 more ...
The most widely used is the gene coding for E. coli β-galactosidase, whose activity can easily be detected by the ability of ... Antibiotic resistance is often used as marker, an example being the beta-lactamase gene, which confers resistance to the ... Cloning is generally first performed using Escherichia coli, and cloning vectors in E. coli include plasmids, bacteriophages ( ... E. coli plasmid vectors. Methods in Molecular Biology. 235. p. 23. ISBN 978-1-58829-151-6. Romanos MA, Scorer CA, Clare JJ ( ...
Earliest discoveries of transferase activity occurred in other classifications of enzymes, including Beta-galactosidase, ... This enzyme is a component of MoCo biosynthesis in Escherichia coli. The reaction it catalyzes is as follows: adenylyl- ... Nichols JD, Xiang S, Schindelin H, Rajagopalan KV (Jan 2007). "Mutational analysis of Escherichia coli MoeA: two functional ... Forchhammer K, Böck A (Apr 1991). "Selenocysteine synthase from Escherichia coli. Analysis of the reaction sequence". The ...
Enterohaemorrhagic Escherichia coli (EHEC) survives exposure to acute acid stress during gastric passage and progresses to ... 1980;). In vitro gene fusions that join an enzymatically active beta-galactosidase segment to amino-terminal fragments of ... 2000;). One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A97:6640 ... 2004;). Escherichia coli acid resistance: tales of an amateur acidophile. Nat Rev Microbiol2:898-907 [CrossRef][PubMed] ...
Description of Beta Galactosidase , Details of Beta Galactosidase , Article on Beta Galactosidase , Essay on Beta Galactosidase ... Learn more about Beta Galactosidase , Meaning of Beta Galactosidase , ... In E. coli, the lacZ gene is the structural gene for β-galactosidase; which is present as part of the inducible system lac ... A few are evolved beta-galactosidase (EBG), beta-glucosidase, 6-phospho-beta-galactosidase, beta-mannosidase, and lactase- ...
The purpose of this study was to investigate a novel suicide gene therapy using E. coli beta-galactosidase (beta-gal) as the ... Suicide gene therapy using E. coli beta-galactosidase.. Farquhar D1, Pan BF, Sakurai M, Ghosh A, Mullen CA, Nelson JA. ... E. coli beta-gal may be useful as a prodrug-activating enzyme in suicide gene therapy and has the potential to increase the ... Daun02 was a good substrate for beta-gal. By comparison, gal-DCN4 was a poor substrate. Except for PC3, the beta-gal-transduced ...
... coli ,/em, beta Galactosidase protein. Ab79449 is a full length protein produced in Escherichia coli. Abcam provides free ... Recombinant E. coli beta Galactosidase protein. See all beta Galactosidase proteins and peptides. ... Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose ... It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be ...
Escherichia coli. Shigella boydii. Escherichia coli H736. Escherichia coli (strain B / BL21-DE3). Escherichia coli H386. ... Escherichia coli. Shigella boydii. Escherichia coli H736. Escherichia coli (strain B / BL21-DE3). Escherichia coli H386. ... Escherichia coli 1-110-08_S3_C1. And more. 1,024. UniRef90_P00722. Cluster: Beta-galactosidase. 1,630. ... Escherichia coli 1-110-08_S3_C1. And more. 1,024. UniRef50_P00722. Cluster: Beta-galactosidase. 2,949. ...
High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a ...
Bacteria Escherichia coli. ID: 102048 Ratio between number of beta-galactosidase proteins produced per E. Coli RNAP transcript ... Bacteria Escherichia coli. Reference. X. Sunney Xie, Paul J. Choi, Gene-Wei Li, Nam Ki Lee, and Giuseppe Lia, Single-Molecule ... Beta-galactosidase tetramers per induced cell. Value. 10000 unitless Organism. ... Transcription and translation initiation frequencies of the Escherichia coli lac operon. J. Mol. Biol. 114:1-21PubMed ID409848 ...
Inhibition of the enzyme beta-galactosidase from Escherichia coli was determined at 1 mM concentration of the compound; No ...
Coli) Protein for Western Blot Western control BGAL11-C Beta-Galactosidase (E. Coli) Protein for Western Blot Western control ... Beta-Galactosidase, MBP, VSV) > Beta-Galactosidase (E. Coli) Protein for Western Blot (Western control) A-Z Product Search ... Beta-Galactosidase (E. Coli) Protein for Western Blot. Catalog#. Product Description. Product Type. Antibody Type. Size. Price ... Beta-Galactosidase (E. Coli) Protein for Western Blot. Western control. 100 ul. $195.00 ...
Coli) IgG-HRP conjugate Antibody conjugate BGAL12-HRP Anti Beta-Galactosidase (E. Coli) IgG-HRP conjugate Antibody conjugate ... Beta-Galactosidase, MBP, VSV) > Anti Beta-Galactosidase (E. Coli) IgG-HRP conjugate (Antibody conjugate) A-Z Product Search ... Anti Beta-Galactosidase (E. Coli) IgG-HRP conjugate. Antibody conjugate. Rabbit-Poly. 100 ul. $245.00 ...
Anti-lacZ, Anti-b0344, Anti-JW0335, EC=3.2.1.23, Anti-Lactase, Anti-Beta-gal, Anti-Beta-galactosidase, HRP. ...
Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. ... High purity β-galactosidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. ... One Unit of β-galactosidase activity is defined as the amount of enzyme required to release one µmole of p-nitrophenol per ... Selectivity for lactose in the presence of structurally similar oligosaccharides was provided by using a β-galactosidase with ...
... in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hy ... Beta galactosidase is coded by a gene (lac z) ... beta-gal expressing tissue / Available. Enlarge image Beta-gal ... Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose ... Anti-beta-gal (Clone 3A9A) immunohistochemistry in mouse paraffin tissues.. In the present mouse model, Beta galactosidase ...
High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a ... E. COLI BETA-GALACTOSIDASE AT 1.7 ANGSTROM. Display Files *FASTA Sequence. *PDB File ... 2 Overlay of the protein model for B. circulans rBgaD-50-650 in yellow and E.coli β-galactosidase crystal structure (PDB: 1DP0 ... Biochemical characterization of mutants in the active site residues of the ?-galactosidase enzyme of Bacillus circulans ATCC ...
Beta-galactosidase. Escherichia coli O16:H48. 1030. Beta-galactosidase. Escherichia coli O80:H26 ... tr,Q7MI75,Q7MI75_VIBVY Beta-galactosidase OS=Vibrio vulnificus (strain YJ016) OX=196600 GN=VV2642 PE=3 SV=1 ... Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.UniRule annotation. ,p>Information which ... IPR036156 Beta-gal/glucu_dom_sf. IPR011013 Gal_mutarotase_sf_dom. IPR008979 Galactose-bd-like_sf. IPR014718 GH-type_carb-bd. ...
Beta-Galactosidase). Ab54101 is a full length protein produced in Escherichia coli and has been validated in… ... Recombinant Treponema Membrane Protein A (Beta-Galactosidase). See all Treponema Membrane Protein A proteins and peptides. ...
Beta-galactosidase. Escherichia coli O1:K1 / APEC. Loading... A1A9U6 Glucans biosynthesis protein G. Escherichia coli O1:K1 / ... Beta-galactosidase. Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / DSM 30187 / JCM 1027 / KCTC 2358 / NCIMB 9240). ... Beta-galactosidase. Yersinia enterocolitica serotype O:8 / biotype 1B (strain NCTC 13174 / 8081). Loading... ... Beta-galactosidase. Yersinia pestis (strain Pestoides F). Loading... A4W7D2 Beta-galactosidase. Enterobacter sp. (strain 638). ...
Entire Escherichia coli beta-galactosidase bound to phenylethyl beta-D-t.... Entire. Name: Escherichia coli beta-galactosidase ... Escherichia coli beta-galactosidase bound to phenylethyl beta-D-thiogalactopyranoside (PETG):. beta-galactosidase. / ligand. ... beta-galactosidase complex / b-galactosidase / beta-galactosidase activity / carbohydrate binding / magnesium ion binding / ... Component #1: protein, beta-galactosidase. Protein. Name: beta-galactosidase. / a.k.a: beta-gal, b-gal / Oligomeric Details: ...
... beta.-galactosidase. The process utilizes .beta.-galactosidase, an enzyme usually used for bond breaking, to form a bond ... 3glycal disaccharides such as Gal.beta.1,3Glucal, an intermediate useful in Le.sup.a preparation and an inhibitor of . ... beta.-Galactosidase (E. coli) lactose Glucal Gal.beta.1,3Glucal and Gal.beta.1,6Glucal .beta.-Galactosidase (E. coli) Gal.beta. ... beta.-Galactosidase (E. coli) Gal.beta.pNO.sub.2 Ph Fucal Hydrolysis to Gal .beta.-Galactosidase (E. coli) Gal.beta.pNO.sub.2 ...
Beta-galactosidase assays.The beta-galactosidase assays were performed as previously described (43). Briefly, appropriate ... Glycolytic and gluconeogenic growth of Escherichia coli O157:H7 (EDL933) and E. coli K-12 (MG1655) in the mouse intestine. ... The beta-galactosidase assay was performed at an OD600 of 0.5. (C and D) To investigate direct binding, EMSAs were performed ... We transformed an espFu::lacZ transcriptional fusion into the single and double mutants and performed beta-galactosidase assays ...
On the nature of the repressor of beta-galactosidase synthesis in Escherichia coli. Biochim. Biophys. Acta 36:545-547. ... beta-Gal, β-galactosidase. (b) Schematic maps of pUT18C-ftsI and pUT18C-ftsI/ftsL plasmids show locations of the T18 gene-ftsI ... b) Functional complementation between the indicated hybrid proteins was quantified by measuring β-galactosidase (beta-Gal) ... BACTH analysis of E. coli cell division proteins.To characterize the physical associations between components of the E. coli ...
RELION-3.0 reconstruction of beta-galactosidase particles in EMPIAR-10061 ... Proteins: Escherichia coli beta-galactosidase bound to phenylethyl beta-D-thiogalactopyranoside (PETG), Escherichia coli beta- ... Sample name: Escherichia coli beta-galactosidase bound to phenylethyl beta-D-thiogalactopyranoside (PETG) ...
... in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and… ... Beta-galactosidase is coded by a gene (lac z) ... Beta-galactosidase tag antibody Beta-galactosidase tag antibody ... Lane 1: Beta-galactosidase. Load: 50 ng per lane. Primary antibody: Beta-galactosidase (E. coli) antibody biotin conjugated Cat ... Anti Beta-galactosidase antibody recognizes the enzyme beta-galactosidase, or ß-galactosidase, that is a component of assays ...
Original antibody is raised against beta-galactosidase. (RAB00031) - Products - Abnova ... Recombinant His-tagged mouse Fab fragment raised against beta-galactosidase. ... Escherichia coli *Specificity:. *This Fab fragment recognizes wild-type E. coli beta-galactosidase. ...
lacZ, beta-galactosidase, E. coli. Expressed Gene. FUS, fused in sarcoma , human. ... CAG, CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter, ... the CMV-IE enhancer/chicken beta-actin hybrid promoter (CAG), a loxP site, the β-galactosidase gene (lacZ) with nuclear ... This transgene consists of the cytomegalovirus immediate early enhancer-chicken beta-actin hybrid (CAG) promoter, a floxed lacZ ...
  • The amino-terminal sequence of β-galactosidase, the α-peptide involved in α-complementation, participates in a subunit interface. (wikipedia.org)
  • High resolution refinement of beta-galactosidase in a new crystal form reveals multiple metal-binding sites and provides a structural basis for alpha-complementation. (openwetware.org)
  • This phenomenon of α-complementation was first demonstrated in work done by Agnes Ullmann in the laboratory of François Jacob and Jacques Monod , where the function of an inactive mutant β-galactosidase with deleted sequence was shown to be rescued by a fragment of β-galactosidase in which that same sequence, the α-donor peptide, is still intact. (wikipedia.org)
  • In this method, DNA ligated into the plasmid disrupts the α peptide and therefore the complementation process, and no functional β-galactosidase can form. (wikipedia.org)
  • The rescue of function of the mutant β-galactosidase by the α-peptide is called α-complementation. (wikipedia.org)
  • Later, Vieira and colleagues (Vieira & Messing 1982) realised that α-complementation could be used to screen E. coli colonies for the presence of inserts. (sigmaaldrich.com)
  • This was shown by using the example of alpha and omega complementation of beta-galactosidase activity. (igem.org)
  • In Escherichia coli , the cell division process, also referred to as cytokinesis, constriction, or septation, is one of the most central yet poorly understood aspects of the bacterial physiology (for reviews, see references 5 , 33 , and 45 ). (asm.org)
  • Which part of the bacterial structure contains Beta-lactamases? (brainscape.com)
  • These enzymes also include an immunoglobulin-like beta-sandwich domain. (wikipedia.org)
  • 1. A process for producing a galactosyl.beta.1,3glycal disaccharide that comprises admixing (i) a galactoside donor molecule, (ii) glucal, 6-O--C.sub.1 -C.sub.6 acyl glucal or6-O--C.sub.1 -C.sub.6 acyl galactal as acceptor and (iii) a catalytic amount of .beta. (patentgenius.com)
  • 10. A process for producing Gal.beta.1,3-(6-O-Ac)Glucal that comprises admixing p-nitrophenyl galactopyranoside, 6-O-acetyl-glucal and a catalytic amount of .beta. (patentgenius.com)
  • The catalytic domain of Beta-galactosidases have a TIM barrel core surrounded several other largely beta domains. (wikipedia.org)
  • High purity β-galactosidase ( Escherichia coli ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • Competitive uptake assays showed that E. coli cells expressing invasin pstb had a significant advantage in the internalization process versus either E. coli cells expressing invasin ent or the invasin pstb derivatives deleted for D2, further demonstrating the importance of invasin self-interaction for the efficiency of invasin-mediated uptake. (asm.org)
  • coli has been clearly elucidated with so many crystallographic structures such as PDB 1DP0 , 1JYN and 1JYV. (rcsb.org)
  • Here we report the structure of a complex between Escherichia coli β-galactosidase and the cell-permeant inhibitor phenylethyl β-D-thiogalactopyranoside (PETG), determined by cryo-EM at an average resolution of ~2.2 angstroms (Å). (pdbj.org)
  • An enzymatic process is disclosed for the preparation of galactosyl.beta.1,3glycal disaccharides such as Gal.beta.1,3Glucal, an intermediate useful in Le.sup.a preparation and an inhibitor of .beta. (patentgenius.com)