The chilling of a tissue or organ during decreased BLOOD perfusion or in the absence of blood supply. Cold ischemia time during ORGAN TRANSPLANTATION begins when the organ is cooled with a cold perfusion solution after ORGAN PROCUREMENT surgery, and ends after the tissue reaches physiological temperature during implantation procedures.
A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.
The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).
An absence of warmth or heat or a temperature notably below an accustomed norm.
Solutions used to store organs and minimize tissue damage, particularly while awaiting implantation.
Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.
A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.
A disorder of cardiac function caused by insufficient blood flow to the muscle tissue of the heart. The decreased blood flow may be due to narrowing of the coronary arteries (CORONARY ARTERY DISEASE), to obstruction by a thrombus (CORONARY THROMBOSIS), or less commonly, to diffuse narrowing of arterioles and other small vessels within the heart. Severe interruption of the blood supply to the myocardial tissue may result in necrosis of cardiac muscle (MYOCARDIAL INFARCTION).
Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.
General dysfunction of an organ occurring immediately following its transplantation. The term most frequently refers to renal dysfunction following KIDNEY TRANSPLANTATION.
The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.
Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.
A tissue or organ remaining at physiological temperature during decreased BLOOD perfusion or in the absence of blood supply. During ORGAN TRANSPLANTATION it begins when the organ reaches physiological temperature before the completion of SURGICAL ANASTOMOSIS and ends with reestablishment of the BLOOD CIRCULATION through the tissue.
The transference of a kidney from one human or animal to another.
The transference of a part of or an entire liver from one human or animal to another.
A XANTHINE OXIDASE inhibitor that decreases URIC ACID production. It also acts as an antimetabolite on some simpler organisms.
The administrative procedures involved with acquiring TISSUES or organs for TRANSPLANTATION through various programs, systems, or organizations. These procedures include obtaining consent from TISSUE DONORS and arranging for transportation of donated tissues and organs, after TISSUE HARVESTING, to HOSPITALS for processing and transplantation.
A dead body, usually a human body.
Elements of limited time intervals, contributing to particular results or situations.
A catarrhal disorder of the upper respiratory tract, which may be viral or a mixed infection. It generally involves a runny nose, nasal congestion, and sneezing.
Transplantation between genetically identical individuals, i.e., members of the same species with identical histocompatibility antigens, such as monozygotic twins, members of the same inbred strain, or members of a hybrid population produced by crossing certain inbred strains.
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
A local anesthetic of the ester type that has a slow onset and a short duration of action. It is mainly used for infiltration anesthesia, peripheral nerve block, and spinal block. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1016).
The procedure of removing TISSUES, organs, or specimens from DONORS for reuse, such as TRANSPLANTATION.
Organs, tissues, or cells taken from the body for grafting into another area of the same body or into another individual.
A vasodilator used in angina of effort or ischemic heart disease.
A state of prolonged irreversible cessation of all brain activity, including lower brain stem function with the complete absence of voluntary movements, responses to stimuli, brain stem reflexes, and spontaneous respirations. Reversible conditions which mimic this clinical state (e.g., sedative overdose, hypothermia, etc.) are excluded prior to making the determination of brain death. (From Adams et al., Principles of Neurology, 6th ed, pp348-9)
Irreversible cessation of all bodily functions, manifested by absence of spontaneous breathing and total loss of cardiovascular and cerebral functions.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
Damage to the MYOCARDIUM resulting from MYOCARDIAL REPERFUSION (restoration of blood flow to ischemic areas of the HEART.) Reperfusion takes place when there is spontaneous thrombolysis, THROMBOLYTIC THERAPY, collateral flow from other coronary vascular beds, or reversal of vasospasm.
A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.
The transference of a pancreas from one human or animal to another.
An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.
A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.
A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.
Abnormally low BODY TEMPERATURE that is intentionally induced in warm-blooded animals by artificial means. In humans, mild or moderate hypothermia has been used to reduce tissue damages, particularly after cardiac or spinal cord injuries and during subsequent surgeries.
A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.
Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.
A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.
Brief reversible episodes of focal, nonconvulsive ischemic dysfunction of the brain having a duration of less than 24 hours, and usually less than one hour, caused by transient thrombotic or embolic blood vessel occlusion or stenosis. Events may be classified by arterial distribution, temporal pattern, or etiology (e.g., embolic vs. thrombotic). (From Adams et al., Principles of Neurology, 6th ed, pp814-6)
Restoration of blood supply to tissue which is ischemic due to decrease in normal blood supply. The decrease may result from any source including atherosclerotic obstruction, narrowing of the artery, or surgical clamping. It is primarily a procedure for treating infarction or other ischemia, by enabling viable ischemic tissue to recover, thus limiting further necrosis. However, it is thought that reperfusion can itself further damage the ischemic tissue, causing REPERFUSION INJURY.
Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.
Non-cadaveric providers of organs for transplant to related or non-related recipients.
Treatment process involving the injection of fluid into an organ or tissue.
Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.
Severe inability of the LIVER to perform its normal metabolic functions, as evidenced by severe JAUNDICE and abnormal serum levels of AMMONIA; BILIRUBIN; ALKALINE PHOSPHATASE; ASPARTATE AMINOTRANSFERASE; LACTATE DEHYDROGENASES; and albumin/globulin ratio. (Blakiston's Gould Medical Dictionary, 4th ed)
The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements.
A technique in which tissue is rendered resistant to the deleterious effects of prolonged ISCHEMIA and REPERFUSION by prior exposure to brief, repeated periods of vascular occlusion. (Am J Physiol 1995 May;268(5 Pt 2):H2063-7, Abstract)
A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.
A climate characterized by COLD TEMPERATURE for a majority of the time during the year.
Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
The transference of a heart from one human or animal to another.
A subfamily of the Muridae consisting of several genera including Gerbillus, Rhombomys, Tatera, Meriones, and Psammomys.
Reduced blood flow to the spinal cord which is supplied by the anterior spinal artery and the paired posterior spinal arteries. This condition may be associated with ARTERIOSCLEROSIS, trauma, emboli, diseases of the aorta, and other disorders. Prolonged ischemia may lead to INFARCTION of spinal cord tissue.
Excision of kidney.
Pathologic processes that affect patients after a surgical procedure. They may or may not be related to the disease for which the surgery was done, and they may or may not be direct results of the surgery.
The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.
The transference of either one or both of the lungs from one human or animal to another.
Exposure of myocardial tissue to brief, repeated periods of vascular occlusion in order to render the myocardium resistant to the deleterious effects of ISCHEMIA or REPERFUSION. The period of pre-exposure and the number of times the tissue is exposed to ischemia and reperfusion vary, the average being 3 to 5 minutes.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.
Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.
Pathological processes of the LIVER.
A general term for the complex phenomena involved in allo- and xenograft rejection by a host and graft vs host reaction. Although the reactions involved in transplantation immunology are primarily thymus-dependent phenomena of cellular immunity, humoral factors also play a part in late rejection.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
Drugs intended to prevent damage to the brain or spinal cord from ischemia, stroke, convulsions, or trauma. Some must be administered before the event, but others may be effective for some time after. They act by a variety of mechanisms, but often directly or indirectly minimize the damage produced by endogenous excitatory amino acids.
A white crystal or crystalline powder used in BUFFERS; FERTILIZERS; and EXPLOSIVES. It can be used to replenish ELECTROLYTES and restore WATER-ELECTROLYTE BALANCE in treating HYPOKALEMIA.
NECROSIS occurring in the MIDDLE CEREBRAL ARTERY distribution system which brings blood to the entire lateral aspects of each CEREBRAL HEMISPHERE. Clinical signs include impaired cognition; APHASIA; AGRAPHIA; weak and numbness in the face and arms, contralaterally or bilaterally depending on the infarction.
Generally, restoration of blood supply to heart tissue which is ischemic due to decrease in normal blood supply. The decrease may result from any source including atherosclerotic obstruction, narrowing of the artery, or surgical clamping. Reperfusion can be induced to treat ischemia. Methods include chemical dissolution of an occluding thrombus, administration of vasodilator drugs, angioplasty, catheterization, and artery bypass graft surgery. However, it is thought that reperfusion can itself further damage the ischemic tissue, causing MYOCARDIAL REPERFUSION INJURY.
Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.
A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.
An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.
An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.
Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)
The circulation of blood through the BLOOD VESSELS of the BRAIN.
Criteria and standards used for the determination of the appropriateness of the inclusion of patients with specific conditions in proposed treatment plans and the criteria used for the inclusion of subjects in various clinical trials and other research protocols.
The circulation of blood through the CORONARY VESSELS of the HEART.
A repeat operation for the same condition in the same patient due to disease progression or recurrence, or as followup to failed previous surgery.
The proportion of survivors in a group, e.g., of patients, studied and followed over a period, or the proportion of persons in a specified group alive at the beginning of a time interval who survive to the end of the interval. It is often studied using life table methods.
Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.

Calcium channel blocker and renal mitochondrial function in warm renal ischemia. (1/125)

OBJECTIVE: Ions, particularly calcium ions, play an important role in ischemia-reperfusion cell injury. In this study, we investigated the action of verapamil on the mitochondrial function of kidneys submitted to ischemia without blood reperfusion in order to study isolated early and late ischemic effects. MATERIALS AND METHODS: 44 rats were submitted to bilateral warm renal ischemia for 30 minutes. The kidneys were then immediately reperfused with saline or Euro-Collins (EC) solution, with and without previous administration of 0.35 mg/kg of verapamil. Mitochondrial function was assessed at the end of renal perfusion and after 24 hours of cold preservation. RESULTS: In kidneys perfused with saline, verapamil allowed a significant early preservation of state III mitochondrial respiration, a result that was no longer evident after 24 hours. In kidneys perfused with EC solution, verapamil did not change state III for either early or late evaluations. Comparison of the groups showed that the results obtained for kidneys perfused with EC were always superior to those obtained for the saline group, except for the initial analysis of kidneys treated with saline and verapamil, which showed results similar to those obtained with EC perfusion alone. CONCLUSION: Administration of verapamil before warm ischemia provides partial and short-lasting functional protection of the mitochondrial function in kidneys perfused with sodium rich saline. With Euro-Collins solution, verapamil did not show any additional beneficial effect. This fact permits us to conclude that protective action is effective only under conditions that facilitate increased sodium uptake and/or potassium loss.  (+info)

Major effects of delayed graft function and cold ischaemia time on renal allograft survival. (2/125)

BACKGROUND: There is mounting evidence from experimental and clinical studies that the quality of organs from cadaver donors may be influenced by events occurring around the time of brain death, and that these may affect transplant outcome. The aim of this study is to investigate the influence of donor factors on renal allograft outcome in a homogeneous cohort of 518 patients transplanted in a single centre over a 9 year period. METHODS: Endpoints of the study were delayed graft function (DGF), acute rejection (AR), 1 year graft survival and long-term survival of those grafts that reached 1 year. Multivariate analysis was performed to determine factors that may have influenced the graft outcome indicators. RESULTS: DGF was the major predictor of graft failure overall with cold ischaemia time (CIT) as an important independent factor. The level of histocompatibility did not influence graft survival. DGF was the major factor affecting 1 year graft survival (P<0.0005) with effects persisting beyond 1 year. DGF was significantly influenced by CIT, donor age, female kidney into male recipient and donor creatinine (P<0.05). Other donor factors and factors associated with donor management were not risk factors for DGF, rejection episodes or graft survival. The risk factors for a number of AR episodes were HLA-DR mismatch and DGF (P<0.005). When grafts surviving for 1 year were considered, only CIT, recipient age and creatinine at 1 year (P<0.05) were found to affect graft survival significantly. CONCLUSIONS: The results of this analysis of well-matched transplant recipients show that CIT and DGF are the most important predictors of poor short and long-term graft survival. Therefore, in order to improve the long-term survival of renal allografts efforts should focus on limiting CIT and the damage that occurs during this period and on improving our understanding of DGF.  (+info)

Glycine intravenous donor preconditioning is superior to glycine supplementation to low-potassium dextran flush preservation and improves graft function in a large animal lung transplantation model after 24 hours of cold ischemia. (3/125)

OBJECTIVES: The potential role of glycine in combination with standard lung preservation with low-potassium dextran solution in lung ischemia-reperfusion injury has not been investigated in a preclinical porcine transplant model. METHODS: In a control group (n = 6), donor lungs were flushed with 1 liter of low-potassium dextran solution. In a second group (LPD-glyc, n = 6), low-potassium dextran solution was supplemented with 3.75 g of glycine. In a third group (IV-glyc, n = 6), donor preconditioning was performed by intravenous administration of 3.75 g glycine 1 hour before low-potassium dextran preservation. Grafts were stored in low-potassium dextran at 4 degrees C for 24 hours. Posttransplant graft function was assessed throughout a 7-hour observation period. RESULTS: In the control group, 2 recipients died of right-sided heart failure caused by severe ischemia-reperfusion injury. All animals of the glycine groups survived the entire observation period. Pulmonary vascular resistance remained significantly (P < .01) lower in both glycine groups when compared with controls. At the end of the observation period pulmonary vascular resistance in the control group was higher (P < .01) compared with the glycine groups (1310 +/- 319 dyn x sec x cm(-5) vs 879 +/- 127 dyn x sec x cm(-5) [LPD-glyc] vs 663 +/- 191 dyn x sec x cm(-5) [IV-glyc]). Changes of lung tissue water content were lower in the IV-glyc group compared with the LPD-control (P < .01) and LPD-glyc lungs (P < .05). Oxygenation (PO2/FiO2) was higher in the IV-glyc group compared with the LPD-glyc and control lungs (445 +/- 110 mm Hg vs 388 +/- 124 mm Hg [P < .01] vs 341 +/- 224 mm Hg [P < .001], respectively). DISCUSSION: Modification of low-potassium dextran solution with glycine or donor preconditioning ameliorates ischemia-reperfusion injury in lung transplantation. This intriguing approach merits further evaluation with respect to the mechanisms involved and may improve results in clinical lung preservation.  (+info)

In situ demonstration of improvement of liver mitochondria function by melatonin after cold ischemia. (4/125)

In a previous investigation, reperfusion with a melatonin-containing medium was demonstrated to enhance bile production and tissue ATP levels in rat livers, cold-preserved with University of Wisconsin (UW) or Celsior solutions, with respect to melatonin-free reperfusion; lipid peroxidation products in the perfusate were not influenced by the indole. This was ascribed to an increased efficiency of the hepatocyte mitochondria induced by melatonin. Reactive oxygen species (ROS) normally leak from the electron transfer chain in mitochondria and excessive ROS production is presumed to mediate ischemia-reperfusion (I/R) damage. A histochemical reaction was used to demonstrate ROS on the same model. Compared to the lobular zonation of ROS in control livers, the stained area of cold-preserved livers reperfused without melatonin was restricted to a narrow portal region, in keeping with the much lower ATP content. When reperfusion was performed with melatonin, the liver morphology was improved and the ROS reaction in hepatocytes more intense, though not reaching the control liver pattern. Sinusoidal cells were poorly-stained in both cases. In conclusion, with this different approach, melatonin was confirmed to improve mitochondrial performance and to discriminate parenchymal from sinusoidal cell behavior. Our observations confirm that melatonin mitigates I/R injury and support its potential in liver transplantation.  (+info)

Cardioprotective effects of tetrahydrobiopterin in cold heart preservation after cardiac arrest. (5/125)

BACKGROUND: It has recently been shown that tetrahydrobiopterin (BH4), an essential cofactor of nitric oxide synthase (NOS), reduces ischemia-reperfusion myocardial injury. The aim of this study was to determine if supplementation with BH4 after cardiac arrest followed by cold heart preservation would exert a cardioprotective effect against ischemia-reperfusion injury. MATERIALS AND METHODS: Isolated perfused rat hearts were subjected to 4 degrees C cold ischemia and reperfusion. Hearts were treated with cold cardioplegic solution with or without BH4 just before ischemia and during the first 5 min of reperfusion period. Effects of BH4 on left ventricular function, myocardial contents of high-energy phosphates, and nitrite plus nitrate were measured in the perfusate, before ischemia and after reperfusion. Moreover, the effect of BH4 on the cold-heart preservation followed by normothermic (37 degrees C) ischemia was determined. RESULTS: BH4 improved the contractile and metabolic abnormalities in reperfused cold preserved hearts that were subjected to normothermic ischemia. Furthermore, BH4 significantly alleviated ischemic contracture during ischemia, and restored the diminished perfusate levels of nitrite plus nitrate after reperfusion. CONCLUSION: These results demonstrated that BH4 reduces ischemia-reperfusion injury in cold heart preservation. The cardioprotective effect of BH4 implies that BH4 could be a novel and effective therapeutic option in the preservation treatment of donor heart after cardiac arrest.  (+info)

Mediators of rat ischemic hepatic preconditioning after cold preservation identified by microarray analysis. (6/125)

Hepatic ischemia-reperfusion injury associated with liver transplantation is an as yet unresolved problem in clinical practice. Preconditioning protects the liver against the deleterious effects of ischemia, although the mechanism underlying this preconditioning is still unclear. To profile gene expression patterns involved in hepatic ischemic preconditioning, we analyzed the changes in gene expression in rat livers by DNA microarray analysis. Approximately 116 genes were found to have altered gene expression after 8 hours of cold ischemia. Moreover, the expression of 218 genes was modified by classic preconditioning followed by the same ischemia process. Given the importance of the effects of ischemic preconditioning (IP) in minimizing the liver damage induced by sustained ischemia before reperfusion, this study analyzed the putative genes involved in the beneficial role of IP in liver grafts undergoing cold ischemia before its implantation in the recipient (IP+I). Great differences were found in the gene expression pattern of ischemic preconditioning + long cold ischemia (IP+I) group when compared with the long cold ischemia alone condition (I), which could explain the protective regulatory mechanisms that take place after preconditioning. Twenty-six genes that were downregulated in cold ischemia were found upregulated after preconditioning preceding a long cold ischemia period. These would be genes activated or maintained by preconditioning. Heat shock protein genes and 3-hydroxy-3-methylglutaryl-coenzyme A reductase are among the most markedly induced transcripts.  (+info)

Prolonging warm ischemia reduces the cold preservation limits of liver grafts in swine. (7/125)

BACKGROUND: The critical shortage of transplantable organs necessitates utilization of unconventional donors. But the safe time limits of cold preservation of liver grafts subjected to warm ischemia (WI) for up to 30 minutes from non-heart-beating-donors (NHBDs) has not been delineated. In this study, we investigated how the limits of cold ischemia (CI) in University of Wisconsin (UW) solution are changed in liver grafts subjected to WI from 10 to 30 minutes. METHODS: A simple porcine NHBD liver transplantation (LT) model was developed. In donors, livers were subjected to 10, 20 or 30 minutes of WI and subsequent different times of CI in UW solution. Animals were divided into three groups (WI 10 min, WI 20 min, WI 30 min, n=13 in each group) and nine subgroups (from CI 6 h to CI 28 h). One-week survival rates of recipients, hepatic function, liver energy metabolism, grafted liver microcirculation and pathological observations of the liver were compared. RESULTS: In the WI 10 min group, the one-week survival rate of the CI 20 h subgroup was significantly higher than in the other two subgroups (CI 24 h and CI 28 h) (P<0.05). Furthermore, the CI 20 h subgroup had a lower level of alanine aminotransferase (ALT), less pathological damage, a higher concentration of adenosine triphosphate (ATP) and microcirculatory blood flow in the grafted livers at 1 hour after reperfusion than the other two subgroups. The same trends were also found in the other two groups (WI 20 min and WI 30 min) and their subgroups. CONCLUSIONS: The cold preservation limits of the liver grafts shortened from 20 to 12 to 6 hours when WI time was prolonged from 10 to 20 to 30 minutes. Only the liver grafts within these time limits could be safely transplanted.  (+info)

Novel short-term hypothermic oxygenated perfusion (HOPE) system prevents injury in rat liver graft from non-heart beating donor. (8/125)

OBJECTIVE: To assess a machine perfusion system in rescuing liver grafts from non-heart-beating donors (NHBD). SUMMARY BACKGROUND DATA: The introduction of extracorporeal liver perfusion systems in the clinical routine depends on feasibility. Conceivably, perfusion could be performed during recipient preparation. We investigated whether a novel rat liver machine perfusion applied after in situ ischemia and cold storage can rescue NHBD liver grafts. METHODS: We induced cardiac arrest in male Brown Norway rats by phrenotomy and ligation of the subcardial aorta. We studied 2 experimental groups: 45 minutes of warm in situ ischemia + 5 hours cold storage versus 45 minutes of warm in situ ischemia + 5 hours cold storage followed by 1 hour hypothermic oxygenated extracorporeal perfusion (HOPE). In both groups, livers were reperfused in a closed sanguineous isolated liver perfusion device for 3 hours at 37 degrees C. To test the benefit of HOPE on survival, we performed orthotopic liver transplantation in both experimental groups. RESULTS: After cold storage and reperfusion, NHBD livers showed necrosis of hepatocytes, increased release of AST, and decreased bile flow. HOPE improved NHBD livers significantly with a reduction of necrosis, less AST release, and increased bile flow. ATP was severely depleted in cold-stored NHBD livers but restored in livers treated by HOPE. After orthotopic liver transplantation, grafts treated by HOPE demonstrated a significant extension on animal survival. CONCLUSIONS: We demonstrate a beneficial effect of HOPE by preventing reperfusion injury in a clinically relevant NHBD model.  (+info)

TY - JOUR. T1 - Ex Vivo Transfer of Nuclear Factor-κB Decoy Ameliorates Hepatic Cold Ischemia/Reperfusion Injury. AU - Yoshizumi, Tomoharu. AU - Ikeda, Yasuhiro. AU - Kaneda, Y.. AU - Sueishi, K.. PY - 2009/6/1. Y1 - 2009/6/1. N2 - Cold ischemia/reperfusion injury of the hepatic graft has been attributed to the release of various inflammatory cytokines. Specific inhibition of these cytokines may improve viability of the hepatic graft upon reperfusion. Herein we have assessed the efficacy of cis element decoy against nuclear factor-κB binding site delivery to the hepatic tissue in a rodent liver transplantation model. At 8 hours after reperfusion of the liver, significant reduction was noted in the livers treated with decoy in the release of cytosolic enzymes from the hepatocytes and in serum tumor necrosis factor α (P , .05). The neutrophilic infiltration into the hepatic grafts was significantly suppressed in the livers treated with decoy oligodeoxynucleotides (ODNs). Decoy ODNs against ...
Looking for cold ischemia time? Find out information about cold ischemia time. sequential arrangement of all events, or the interval between two events in such a sequence. The concept of time may be discussed on several different... Explanation of cold ischemia time
TY - CONF. T1 - Gene Expression Profiling of Cold Ischemia/Reperfusion (I/R) Injury in Murine Cardiac Isografts. AU - Schneeberger, S.. AU - Mark, W.. AU - Brandacher, G.. AU - Obrist, Philipp. AU - Lackner, Peter. AU - Hernegger, G.. AU - Meusburger, H.. AU - Renz, O.. AU - Margreiter, R.. AU - Amberger, A. N1 - Supplement to Transplantation. August 27, 2002. Vol. 74, Nr. 4. PY - 2002. Y1 - 2002. M3 - Abstract. ER - ...
The effects of cold storage using Custodiol® (Histidine-Tryptophan-Ketoglutarate, HTK) or isotonic saline solution on mitochondrial function in hearts (left and rights ventricles) and various blood vessels of pigs were investigated. Hearts, saphenous veins, internal-mammary-arteries and aortas of male landrace pigs were harvested and exposed to cold ischemia in either saline or Custodiol-HTK solution. Mitochondrial function was measured in situ in permeabilized fibers by high-resolution respirometry. Mitochondrial respiratory capacities (maximal respiration rates) were similar in the right and left ventricle in controls and after 14 h of cold storage were significantly better preserved in Custodiol-HTK than in saline solution. Mitochondrial respiration rates in various blood vessels including aorta, arteries and veins were less than 5% of myocardium rates. In contrast to the pig heart, in some blood vessels, like veins, mitochondrial function remained stable even after 24 h of cold ischemia. HTK
Background: Deceased-donor (DD) kidneys are at higher risk for ischemia/reperfusion injury leading to increased inflammatory mediators and innate immune response. We aimed to investigate the effects of prolonged cold ischemia (CIT) on intragraft molecular gene expression profiles of DD kidneys comparing to living-donor (LD) pre-implantation biopsies and to investigate the molecular features of DD biopsies that develop delayed graft function (DGF).. Methods: There were 48 pre-implantation kidney biopsy samples (29 LD and 19 DD). The cold ischemia time (CIT) was , 16 hrs in 10 and , 16 hrs in 9 DD kidneys. 9 DD patients developed DGF after transplantation. The gene expression profiles were studied by Affymetrix HuGene 1.0 ST expression arrays.. Results: Ingenuity Pathways Analysis demonstrated that DD pre-implantation biopsies showed increased expression of pathways related to acute inflammatory response, lymphocyte mediated immunity, innate and humoral immune response, complement activation and ...
Based on their potential to analyze aberrant cellular signaling in relation to biological function, kinase activity profiling in tumor biopsies by peptide microarrays and mass spectrometry-based phosphoproteomics may guide selection of protein kinase inhibitors in patients with cancer. Variable tissue handling procedures in clinical practice may influence protein phosphorylation status and kinase activity and therewith may hamper biomarker discovery. Here, the effect of cold ischemia time (CIT) on the stability of kinase activity and protein phosphorylation status in fresh-frozen clinical tissue samples was studied using peptide microarrays and mass spectrometry-based phosphoproteomics. Biopsies of colorectal cancer resection specimens from five patients were collected and snap frozen immediately after surgery and at 6 additional time points between 0 and 180 min of CIT. Kinase activity profiling was performed for all samples using a peptide microarray. MS-based global phosphoproteomics was performed in
Definition of ischemia time in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is ischemia time? Meaning of ischemia time as a legal term. What does ischemia time mean in law?
inproceedings{116331, author = {Troisi, Roberto and MEESTER, D and DE RYCK, F and Cuvelier, Claude and HESSE, UJ}, booktitle = {TRANSPLANTATION}, issn = {0041-1337}, language = {eng}, number = {9}, pages = {S652-S652}, title = {The impact of prolonged cold ischemia on delayed graft function (DGF) in pancreas transplantation.}, volume = {67}, year = {1999 ...
Ischemia and reperfusion are natural steps during kidney transplantation, and IRI is considered one of the most important nonspecific factors affecting allograft dysfunction. Transplanted organs experience several episodes of ischemia, in which cold ischemia occurs during allograft storage in preservation solutions. Even though cold ischemia has been studied extensively, all of the studies have been carried out in vitro and ex vivo models. There is no in vivo model available to examine renal IRI induced solely by cold ischemia. In the present study, we developed an in vivo mouse model to study renal IRI induced exclusively by cold ischemia through clamping the renal pedicle for 1 to 5 hours. During the ischemic phase, blood was flushed from the kidney with cold saline through a small opening on the renal vein. The kidney was kept cold in a kidney cup with circulating cooled saline, while the body temperature was maintained at 37℃ during the experiment. The level of kidney injury was evaluated by
Effect of cold ischemic times and time after transplantation on regional myocardial motion after heart transplantation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
PMID: 18317412 Schneeberger Stefan, Kuznetsov Andrey V, Seiler Rüdiger, Renz Oliver, Meusburger Hugo, Mark Walter, Brandacher Gerald, Margreiter Raimund, Gnaiger Erich (2008) Shock Abstract: Cold ischemia time and preservation of organs are limited by I/R injury leading to primary nonfunction of the graft. In a rat heart transplant model, we compared cardioplegic St Thomas (ST) to histidine-tryptophan-ketoglutarate (HTK) and University of Wisconsin preservation solutions in terms of contractile function, and mitochondrial respiratory and enzymatic defects after prolonged cold ischemia and reperfusion. Contractile function was scored after transplantation and 24 h of reperfusion. Mitochondrial function was investigated by high-resolution respirometry of permeabilized myocardial fibers. Graft performance in terms of contractile function declined with the duration of cold storage. Recovery was significantly improved after 10 h of cold storage in HTK compared with ST (cardiac scores, 3.3 ± 0.5 and ...
Researchers have long considered the NCI supported Cooperative Human Tissue Network (CHTN) as a source for quality collection and preservation of tissue samples from remnant surgical tissue specimens. In an organized tissue procurement setting serving a large population of researchers, the trained procurement personnel, specialized reagents and monitoried frozen storage facilities assure the availability of quality research, monitored preservation techniques and temperature controlled shipping required for successful research support.. The CHTN Midwestern Division (MWD) has experienced an increase in investigator demand for fresh tissues over the past two years. Approximately 23% of the tissues distributed by the CHTN MWD is fresh tissue. Requests for frozen and formlin-fixed paraffin embedded tissues, however, remain constant and stable. The increased interest in fresh tissue likely reflects an increased focus on the study of vital cells in controlled environments for... Read more ...
While the method itself might be close to perfect, upstream (pre-analytic) and downstream (data mining) processes need to be improved.
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Intestinal transplantation is associated with high rates of mortality and morbidity. This paper details our initial experience with 82 heterotopic small bowel transplants based upon the original rat model described by Monchik and Russell (Surgery 70:693-702, 1971). A key issue associated with mortality was a warm ischaemia time of more than 40 min (P | 0.01). Sixty- eight percent of the recipients (44/65) survived for mere than 24 hr when the warm ischaemia time of the donor bowel was reduced to less than 40 min. Investigators establishing an animal model of heterotopic small bowel transplantation should pay particular attention to the warm ischaemia time of the donor bowel.
Cold ischemia time (from flush to out-of-ice) and warm ischemia time (from out-of-ice to reperfusion) are known to impact delayed graft function (DGF) rates and long-term allograft survival following deceased donor kidney transplantation. We propose an additional ischemia time, extraction time, begi …
We previously reported that progression of liver cirrhosis is quicker and survival is dismal in patients with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) coinfection, especially when acquired in childhood through contaminated blood products. Recently, we performed the first deceased donor liver transplantation (DDLT) for an HIV/HCV-coinfected hemophilic patient in Japan. A 40-year-old man was referred to our hospital for liver transplantation. Regular DDLT was performed using the piggyback technique with a full-sized liver graft. Cold ischemia time was 465 min, and the graft liver weighed 1,590 g. The antiretroviral therapy (ART) was switched from darunavir/ritonavir to raltegravir before the transplant for flexible usage of calcineurin inhibitors postoperatively; tenofovir was used as the baseline treatment. The postoperative course was uneventful, and the patient was discharged home on day 43. He started receiving anti-HCV treatment on day 110 with pegylated interferon, ...
Results In the PI arm 11.1% (n=99) had AbMI versus 6.9% (n=59) in primary PCI arm (p,0.01). In a multivariable model, AbMI patients overall had less baseline ΣST-deviation, fewer baseline Q-waves and shorter total ischaemic times. PI AbMI patients had faster time to TNK (90 vs 100 min, p=0.015): total ischaemic time was 100 min longer in primary PCI AbMI patients and no difference in ischaemic time existed between AbMI and non-AbMI patients within this group. Although no significant interaction between treatment and AbMI on the composite endpoint of death/shock/congestive heart failure/recurrent MI occurred (p=0.292), PI AbMI patients had a lower incidence in this endpoint than non-AbMI patients (5.1 vs 12%, p=0.038); this was not evident in primary PCI patients. Forty-five patients (ie, 2.5%) had masquerading MI with minimal biomarker elevation and no evolution in baseline ST-elevation.. ...
Impact of performing multiple liver transplants (LT) in a short period of time is unknown. Consecutively performed LT potentially increase complication rates through team fatigue and overutilization of resources and increase ischemia time. We analyzed the impact of undertaking consecutive LT (Consecutive liver transplant, CLT; LT preceded by another transplant performed not more than 12 h before, both transplants grouped together) on outcomes. Of 1702 LT performed, 314 (18.4%) were CLT. Outcome data was compared with solitary LT (SLT; not more than one LT in 12-h period). Recipient, donor, and graft characteristics were evenly matched between SLT and CLT; second LT of CLT group utilized younger donors grafts with longer cold ischemic times (P = 0.015). Implantation and operative time were significantly lower in CLT recipients on intergroup analysis (P = 0.0001 and 0.002, respectively). Early hepatic artery thrombosis (E-HAT) was higher in CLT versus SLT (P = 0.038), despite absolute number of ...
The results show that human uterine myometrial tissue is resistant towards cold ischaemia for at least 6 h if stored in UW and PER solutions.
Heart transplantation is the final treatment option in heart failure, and transplant patients have substantially increased survival rates and quality of life.27 The first human-to-human heart transplantation was performed in South Africa in 1967, and by the end of 1968 102 patients had received transplants at 52 institutions.1 However, the results were disappointing, with a 60% early death rate and a mean survival of only 29 days.28 In a large study in 2000, when the duration of cold ischaemia was 6 h, graft survival at 3 years after surgery was nearly 25% less than that when the cold ischaemia period was ,2 h.29. Use of organs from marginal donors has resulted in decreased graft function and survival compared with the use of organs from younger donors.30 It is currently difficult to obtain donor hearts. In addition, long-term outcomes for transplanted hearts are limited by the development of cardiac allograft vasculopathy resulting from immunological and non-immunological risk factors ...
View Poster. INTRODUCTION. Marginal kidneys have gained progressive acceptance with the use of extended criteria donor (ECD) kidneys, however there still remains high discard rates of these allografts. Increasing the donor pool can be accomplished through allocation for dual kidney transplantation (DKT) which has shown comparable outcomes to those from single kidney transplantation (SKT). This study analyzed the outcomes of DKT compared to SKT from marginal kidneys.. METHODS. Electronic databases including MEDLINE, EMBASE, PubMed and Cochrane library were searched using Preferred Reporting Items for Systematic Reviews and Meta-Analyses. Each paper included was reviewed in duplicate. Patient factors including mean age of donors and recipients, gender and length of follow up was analyzed. The outcome measures analyzed included the cold ischemia time, graft survival, patient survival, serum creatinine in follow up and delayed graft function (DGF) in DKT compared to SKT. RESULTS. 17 publications ...
Aim: To define regional patterns in post OLT LOS.. Methods: Using the UNOS database (06/2011) we reviewed data on 65,891 OLT recipients, 01/2000-12/2010. Patients excluded (n=11,784) were dual organ, and pediatric recipients, deaths ≤2 days, LOS,180 days or missing data. Variables were categorized (n=54,107): MELD score(≤10, 11-18,19-24, ≥25) Karnofsky score (0-30, 31-60, 61-100), cold ischemia time (CIT, hrs) (,6, 6-12, ,12≤20, ,20). Predictors of LOS were identified univariately, and multivariately using a linear regression model of Log2(LOS). Regional (R) differences in multivariate associations were assessed using parameters for interactions with region, and within regions. The LOS median ratio (MR) was used as a measure of relative change in median LOS.. Results: Mean LOS ranged from 13.3±14(Mean±SD) days in R6 to 19.5±20 days in R9. On multivariate analysis factors associated with ,10% increase LOS were: patient ventilated at OLT (LOS MR estimate 1.33, i.e. 33% increase in LOS), ...
Kraus FB, Ludwig-Kraus B (2018) Measuring zinc on the Roche cobas c502 analyzer-Validation, comparison, and pre-analytic aspects. J Clin Lab Anal Ludwig-Kraus B, Kraus FB (2017) Similar but not consistent: Revisiting the pitfalls of measuring IgG subclasses with different assays. J Clin Lab Anal Kraus FB, Mischereit M, Eller C, Ludwig-Kraus B (2016) Characterization and Validation of the LT-SYS Copper Assay on a Roche Cobas 8000 c502 Analyzer. Laboratory Medicine, lmw049. Bergner M, Seliger G, Schneider U, Stepan H, Kraus FB, Haase R, Wickenhauser C, Hoyer D, Schleussner E, Tchirikov M (2016) Multimodal Monitoring Fetal Inflammation at PPROM - the MuMfI-PPROM-Trial. Geburtshilfe Frauenheilkd. 76- P18. Salt AN, Hartsock JJ, Gill RM, King E, Kraus FB, Plontke SK (2016) Perilymph pharmacokinetics of locally-applied gentamicin in the guinea pig. Hearing Research 342, 101-111. Kapheim KM et al. (2015) Genomic signatures of evolutionary transitions from solitary to group living. Science 348, 1139-1143. ...
Histological Analysis At euthanization, renal tissue specimens were fixed Animal weights did not differ between the groups in neutral 10% buffered formalin and embedded in (MP25, 23.5 ± 4.6 kg; MP30, 26.3 ± 3.2 kg; p = paraffin. Tissue injury was assessed on periodic acid- 0.201). All animals demonstrated normal renal func- Schiff (PAS) stained 4 lm-thick sections by scoring tion before the start of the experiments (serum creati- glomerular damage (shrinking), tubular damage, infil- nine; MP25, 1.09 ± 0.14 mg/dL; MP30, 1.13 ± 0.10 tration of inflammatory cells, interstitial edema and mg/dL; p = 0.565). Cold ischemic times (CITs) necrosis. Tubular damage was defined as dilatation, of the groups were comparable (CIT, MP25, 20:32 h ± denudation of the tubular basement membrane and 0:06; MP30, 20:28 h ± 0:17; p = 0.158). Also, times vacuolization. Injury of each specimen was graded to required for both anastomoses were comparable the extent of region involvement in 10 randomly cho- between the ...
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Background. In renal transplantation, cold ischaemia (CI) determines acute rejection through innate immunity among others. Acute rejection episodes are a risk factor for late allograft dysfunction and proteinuria. This implies some alteration of the glomerular filtration barrier (GFB). Besides its effects on acute rejection, we hypothesized that CI might somehow damage the GFB being directly responsible for late proteinuria.. Methods. On rat kidney allografts suffering from antibody-mediated acute rejection with or without CI and compared with syngeneic grafts, we quantified the gene expression of innate and adaptive immune mediators and assessed the capillary glomerular basement membranes (CapBM) by immunostaining collagen-IV (ColIV). ColIV was also assessed in equivalent groups from a previous chronic study followed up for 24 weeks.. Results. CI up-regulated enzymes critical in the stabilization of collagen chains, increasing ColIV deposition and thickening the CapBM. CI increased the C4d and ...
TY - JOUR. T1 - Clinical application of an extracellular phosphate-buffered solution (EP-TU) for lung preservation. T2 - Preliminary results of a Japanese series. AU - Okada, Yoshinori. AU - Matsumura, Yuji. AU - Date, Hiroshi. AU - Bando, Toru. AU - Oto, Takahiro. AU - Sado, Tetsu. AU - Hoshikawa, Yasushi. AU - Noda, Masafumi. AU - Oishi, Hisashi. AU - Kondo, Takashi. PY - 2012/1. Y1 - 2012/1. N2 - Purpose: We evaluated the effect of an extracellular phosphate-buffered lung preservation solution (EP-TU solution) on acute postoperative graft function and recipient survival in a Japanese series of cadaveric lung transplantation. Methods: The subjects were ten patients who received lung grafts preserved with EP-TU solution at three of four designated lung transplant centers between 2000 and 2005. Three of the patients underwent single lung transplantation and seven underwent bilateral lung transplantation. Postoperative graft function was evaluated by primary graft dysfunction (PGD) grade (0-3), ...
Adhesion molecules play a crucial role in transplant rejection in regulating the interaction of inflammatory cells with cells in the vascular wall. In an aortic transplantation model, we have previously analysed the early adhesion process (7.5 min to 24 h) and the impact of cold ischaemia time (1-24 h) upon transplant arteriosclerosis during the first 2 months after transplantation in the rat. The aim of this investigation was to study adhesion molecules in accelerated transplant arteriosclerosis in a rat model by analysing the immunohistochemical expression of CD11b and ICAM-1 up to 2 months and followed by a semiquantitative evaluation and multivariant analysis. Antigen expression of CD11b and ICAM-1 adhesion molecules was stronger in the aortic allografts than in the ischaemia-induced syngeneic aortic grafts in the whole vessel wall. Neither ICAM-1 nor CD11b antigen expression correlated significantly with time periods of ischaemia/reperfusion injury in allogeneic or syngeneic aortic ...
Date: 19 Mar 96 00:18:15 EST From: Mike Darwin ,. , Subject: BPI TECH BRIEF #18 Cryopreservation of CryoCare Patient #C-2150 by Mike Darwin Introduction On December 12th, 1995 James Gallagher, a 55-year-old software developer from Sunset Beach, California, became CryoCares first member to enter cryopreservation. He also became the first patient ever to benefit from new technologies developed to reduce three forms of injury: * pre-mortem shock * warm ischemia (the time interval between pronouncement of death and restoration of adequate blood circulation) * cold ischemia experienced during initial blood washout and cooling, and also during iced-transport from the location where legal death occurred to the facility where cryoprotective perfusion is carried out. The following is not quite a full technical report, but neither is it simply a lay-level of summary of key events without reference to the technical details and the impact those details had upon this patients care and potentially, future ...
Chill out with our top seven low-potassium fruit salads for a kidney diet. It may be hot outside, but you can keep cool with recipes such as Caramel Apple Salad or Festive Fruit Salad.
Whole grains are high in protein and potassium, with the exception of oatmeal. Processed grains in products such as pasta, bread, bagels and cereal are lower in potassium and suitable for a low-potassium diet. Avoid processed foods that have trans fats, such as crackers and cookies.. ...
This report features 7 companies, including Dr. Franz Köhler Chemie GmbH (Germany), Lifeline Scientific, Inc. (USA), Essential Pharmaceuticals, LLC (USA), Preservation Solutions, Inc. (USA)
TY - GEN. T1 - Rapid chilling of carcasses using vascular perfusion. AU - Brown, T. AU - Richardson, I. AU - Swain, MVL. AU - Evans, JA. N1 - Name and Venue of Event: Naples, Italy, 24-26 September 2007. PY - 2007. Y1 - 2007. M3 - Conference Contribution (Conference Proceeding) BT - Proceeding of the 3rd CIGR Section VI International Symposium on Food and Agricultural Products : Processing and Innovations. ER - ...
How is Long-Lasting Delayed Graft Function abbreviated? LLDGF stands for Long-Lasting Delayed Graft Function. LLDGF is defined as Long-Lasting Delayed Graft Function very rarely.
Description, comparison with clinical data and clinical exploitation of a non-invasive and relatively inexpensive method for screening cardiovascular systems based on multi-parameter analysis of heart haemodynamics and vascular perfusion in selected areas of the body ...
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TY - JOUR. T1 - Protective effect of carbon monoxide inhalation for cold-preserved small intestinal grafts. AU - Nakao, Atsunori. AU - Kimizuka, Kei. AU - Stolz, Donna B.. AU - Neto, Joao Seda. AU - Kaizu, Takashi. AU - Choi, Augustine M.K.. AU - Uchiyama, Takashi. AU - Zuckerbraun, Brian S.. AU - Bauer, Anthony J.. AU - Nalesnik, Michael A.. AU - Otterbein, Leo E.. AU - Geller, David A.. AU - Murase, Noriko. N1 - Funding Information: Supported by NIH Grant DK54232 (Murase), CA76541 (Stolz), HL60234, AI42365, HL55330 (Choi), GM58241 and GM53789 (Bauer), and American Heart 160332U and Atorvastatin Pfizer Research Award (Otterbein) PY - 2003/8/1. Y1 - 2003/8/1. N2 - Background. Heme oxygenase (HO)-1 system has been shown to provide protection against oxidative stress through the degradation of heme to biliverdin, free iron, and carbon monoxide (CO). This study investigated cytoprotective efficacy of CO at a low concentration on cold ischemia/reperfusion (I/R) injury of transplanted intestine. ...
TY - JOUR. T1 - Glutathione supplementation to University of Wisconsin solution causes endothelial dysfunction. AU - Tambyraja, A L. AU - Mitchell, R. AU - Driscoll, P J. AU - Deans, C. AU - Parks, R W. AU - Rahman, I. AU - Megson, I L. PY - 2007. Y1 - 2007. N2 - Glutathione (GSH) is added to University of Wisconsin (UW) organ preservation solution to protect against oxidative stress. This study assesses the effect of GSH-supplementation on endothelial function in tissues subjected to cold ischaemia and compares its effects to a mono-ethyl ester equivalent (GSH-MEE) and S-nitrosated GSH (GSNO).. AB - Glutathione (GSH) is added to University of Wisconsin (UW) organ preservation solution to protect against oxidative stress. This study assesses the effect of GSH-supplementation on endothelial function in tissues subjected to cold ischaemia and compares its effects to a mono-ethyl ester equivalent (GSH-MEE) and S-nitrosated GSH (GSNO).. U2 - 10.1016/j.trim.2007.06.002. DO - ...
Ischemia-reperfusion (I/R) associated with hepatic resection and living related liver transplantation is an unsolved problem in clinical practice. Indeed, I/R induces damage and regenerative failure in clinical liver surgery. Signaling pathways regarding the pathophysiology of liver I/R and regeneration making clear distinction between situations of cold and warm ischemia, as well as liver regeneration with or without vascular occlusion, will be addressed. The different experimental models used to date to improve the postoperative outcomes in clinical liver surgery will be also described. Furthermore, the most updated therapeutic strategies, as well as the clinical and scientific controversies in the field, will be discussed. Such information may be useful to guide the design of better experimental models as well as the effective therapeutic strategies in liver surgery that can succeed in achieving its clinical application.
University of Maryland School of Medicine Background: A full facial procurement should allow concurrent procurement of all solid organs and ensure their integrity. Procurement of facial vascularized composite allografts (VCA) challenges the surgical teams with complex defects potentially requiring far greater time than solid organ procurement. Simultaneous-start procurement in these cases could entail cold ischemia times over 12 hours, risking post-reperfusion bleeding and reperfusion injury.. Methods: A VCA including all facial skin, mimetic muscles, multiple sensory and motor nerve branches, anterior tongue, and maxillary and mandibular segments was procured from a brain dead donor. Solid organ recovery included heart, lungs, liver, kidneys, and pancreas. Bedside tracheostomy and facial mask impression were performed the day before procurement.. Results: Facial VCA dissection time was 12 hours, spaced over a 15 hour period to diminish ischemia while the recipient was being prepared (Fig 1). ...
Double-kidney transplantation is performed using organs from marginal donors with a histological score not suitable for single kidney transplantation. The aim of the study is to verify the results obtained with double-kidney transplantation in terms of graft and patient survival and complications. METHODS: Between September 2001 and September 2004, 16 double-kidney transplantations were performed in our center. The kidneys were all perfused with Celsior solution and the mean cold ischemia time was 17.6+/-2.7 hours. In all cases a pre-transplant kidney biopsy was performed to evaluate the damage. Immunosuppression was tacrolimus based for all patients. RESULTS: Eight patients had good renal postoperative function while the other eight had acute tubular necrosis. Two of the patients who had severe acute tubular necrosis never recovered renal function. There was only one episode of acute rejection, while the incidence of urinary complications was 31.2%; there were two surgical revisions for ...
The incidence of primary graft dysfunction (PDF) after LT is approximately 25% within a range of 9.3 to 43.7% based on different definitions characterizing PDF [1-5]. Initial non-function (INF) develops in up to 6% of all considered cases and requires urgent retransplantation [6].. Risk factors are prolonged cold ischemia time or higher degree of graft steatosis and subsequent development of severe hepatic ischemia/reperfusion injury (IRI) [5, 7]. A cascade of cellular events results in microcirculatory flow disturbance. This may be distinguished as no-flow indicating capillary perfusion failure on the one hand, or reflow-paradox including activation of the leukocyte-endothelium interaction, release of toxic mediators, and impairment of the endothelial barrier on the other hand [8, 9]. There are, obviously, further different factors all contributing to an insufficient function of liver grafts and its detrimental effects on the overall outcome after LT: recipients condition, the donor data, ...
TY - JOUR. T1 - Az ischaemiás posztkondicionálás hatása az oxidatív stresszre és a szöveti struktúrára vékonybél meleg ischaemiás és autotranszplantációs modellekben.. AU - Nedvig, Klára. AU - Völgyi, Edina. AU - Wéber, G.. AU - Rőth, E.. AU - Ferencz, A.. PY - 2011/12. Y1 - 2011/12. N2 - Our study investigated the effect of ischemic postconditioning (IPO) in intestinal warm ischemia/reperfusion (I/R) and autotransplantation models. Warm ischemia was performed by occlusion of superior mesenteric artery for 1, 3 and 6 hours in white domestic pigs (n = 15). Prior to 3 hours reperfusion the intestine was postconditioned by 3 cycles of 30-seconds ischemia and 30-seconds reperfusion (IPO protocol). In the cold ischemia group (n = 15) the bowel was preserved in University of Wisconsin solution for 1, 3, and 6 hours. Prior to 3 hours reperfusion IPO protocol was applied, too. Tissue samples were collected after laparotomy (control) and at the end of the reperfusion periods. As far as ...
Donation after cardiac death (DCD) donors are vital to maximize the organ donor pool. Reperfusion injury (RI) is an important sequela in DCD organs due to warm and cold ischemia. RI manifests clinically as a high incidence of delayed graft function (DGF) and primary non-function (PNF) compared with donation after brain death organs. The importance of nitric oxide (NO) in the generation of reperfusion injury is pivotal ...
Strontium ranelate u common adverse effects of the body and tail, and sometimes leading to copper de ciency leads to a ii (olig4, nkx2.1, nkx2.1) and class ii and pluripotent stem cells (adscs) as a second- her physician makes a toenail thick and thin septa, characteristic of primitive cells. Have served to derive of human anatomy, ed 4, plate 332.) where gases, nutrients, and metabolic support, appropriate anti- microbial drugs. Clarifying stem-cell therapy s benefits and limitations of peptides as drugs. J urol 2005; be achieved by a simple, minimally invasive approaches with cold ischemia has been demonstrated in both groups. 5.1). Behaviour. Continuously with fragmenta- tion except for two years before moving to live longer: Causa- tion of the back. This port placement for urologic surgery tobias simpfend rfer, gencay hatiboglu, boris hadaschik, michael m ller, hans-peter meinzer, jens j. Rassweiler abstract despite continual improvements in treatment of advanced breast adverse effects ...
The research report titled Global Organ Preservation Solutions Market Professional Survey Report 2017 market size and forecast and overview on current market trends
I have a cold storage room underneath my front porch. It is poured out of concrete. It is around 20 long wide by 5 feet Wide. We dont use it at all for cold storage, but want to use it for regular storage year round. Obviously it is very cold in there during the winter.
Magnetic Particle Imaging of the vasculature to assess blood delivery and tissue level perfusion. The direct quantitation of nanoparticle tracers enables accurate perfusion measurements without confounding background tissue signal. Long circulating tracers persist within the vasculature for days, enabling repeated measurements for longitudinal studies. This enables truly functional imaging to measure changes in blood flow and tracer delivery over time in stroke and tumor models. ...
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For liver transplants, the cold ischemia time can be up to 24 hours, although typically surgeons aim for a much shorter period ... This time during transport is called the "cold ischemia time". Heart and lungs should have less than 6 hours between organ ... For kidney transplants, as the cold ischemia time increases, the risk of delayed function of the kidney increases. Sometimes, ... While the organ is being transported, it is either stored in an icy cold solution to help preserve it or it is connected to a ...
Studies on cold-ischemia/eperfusion indicate an ischemic tolerance of more than 24 hours. The recipient has to look at ...
Until this time, kidneys came via the ETF and often had extended cold ischaemia times. Haberal had, in the interim, and before ...
"Each additional hour of cold ischemia time significantly increases the risk of graft failure and mortality following renal ... Concerns regarding the negative impact that Cold Ischemic Time (CIT) may have on graft survival, due to the shipment of the ...
Furthermore, irreversible intestinal damage is seen after approximately only 5 hours of cold ischemia in the form of mucosal ... mesenteric ischemia, motility disorder, omphalocele/gastroschisis, tumors, and volvulus. Regardless of the underlying condition ... and serum pH and lactate levels measured for evidence of intestinal ischemia. The patient's immune system is strongly modulated ... extensive venous thrombosis or arterial ischemia of the mesentery, and motility syndromes. Donated intestines, like all organs ...
Areas of infarct or ischemic tissue will remain "cold". Pre- and post-stress thallium may indicate areas that will benefit from ... This produces coronary steal from areas of ischemia where arteries are already maximally dilated. ...
... and the storage solution itself is designed to counteract the unwanted effects of cold ischemia. Although this "static" cold ... The risk of ischemic cholangiopathy increases with longer durations of cold ischemia time, which is the time that the organ ... The donor's blood in the liver will be replaced by an ice-cold organ storage solution, such as UW (Viaspan) or HTK until the ...
... ischemia-, and cold-induced), multiple sclerosis, auto-immune encephalomyelitis, Alzheimer's disease, and Parkinson's disease. ... and cold-air induced asthma (see Exercise-induced bronchoconstriction); and childhood sleep apnea due to adenotonsillar ...
... improve perfusion protocol and find formulations to minimize cold ischemia (a concern for organ transplantation). Dr. Pichugin ...
... cold ischemia MeSH E05.760.833.660 - organ preservation MeSH E05.760.833.890 - semen preservation MeSH E05.799.638.231 - body ...
... though fewer than 16 hours of cold ischemia or 6 hours of warm ischemia leads to the best outcomes. If replantation is not ...
... at the back table in cold ischemia condition for the donor organ and after native organ removal in the recipient. This is ... finalized to make as brief as possible the donor organ dangerous warm ischemia phase that can be contained in the couple of ...
... cold ischemia MeSH E04.936.450 - organ transplantation MeSH E04.936.450.050 - bone transplantation MeSH E04.936.450.050.100 - ... warm ischemia MeSH E04.950.165 - castration MeSH E04.950.165.679 - orchiectomy MeSH E04.950.165.685 - ovariectomy MeSH E04.950. ...
More specifically, urinary endothelin-1 levels are used as an acute marker in cold ischemic reperfusion and injury. NGAL- ... Kidney ischemia can be diagnosed by checking the levels of several biomarkers such as clusterin and cystatin C. While the ... Kidney ischemia is a disease with a high morbidity and mortality rate. Blood vessels shrink and undergo apoptosis which results ... Moreover, renal ischemia can cause the delay of graft function after renal transplant and can cause rejection of the transplant ...
Symptoms of acute limb ischaemia include: Pain Pallor Paresthesias Perishingly cold Pulselessness Paralysis These symptoms are ... Renal ischemia (nephric ischemia) Mesenteric ischemia Cerebral ischemia Cardiac ischemia In order to treat acute limb ischaemia ... Acute limb ischaemia (ALI) occurs when there is a sudden lack of blood flow to a limb. Acute limb ischaemia is caused by ... The New Latin term ischaemia as written, is a British version of the word ischemia, and stems from the Greek terms ischein 'to ...
Location of a transplant center with respect to a donor hospital is given priority due to the effects of Cold Ischemic Time ( ... kidneys are the most resilient to ischemia.[citation needed] Kidneys packaged on ice can be successfully transplanted 24-36 ... Developments in kidney preservation have yielded a device that pumps cold preservation solution through the kidneys vessels to ... ischemia). Each organ tolerates different ischemic times. Hearts and lungs need to be transplanted within 4-6 hours from ...
... which occurs when blood flow is restored after a period of ischemia, such as on rewarming after a cold episode in patients with ... A cold sore outside the mouth (not a canker sore inside the mouth) can be preceded by tingling because a cold sore is caused by ...
... who experience cold-water drowning do not develop hypothermia quickly enough to decrease cerebral metabolism before ischemia ... The actual cause of death in cold or very cold water is usually lethal bodily reactions to increased heat loss and to freezing ... This is because cold water can have other lethal effects on the body. Hence, hypothermia is not usually a reason for drowning ... Upon submersion into cold water, remaining calm and preventing loss of body heat is paramount. While awaiting rescue, swimming ...
... as in acrobatics and military flying Localized extreme cold, such as by frostbite or improper cold compression therapy ... Ischemia of the small bowel is called mesenteric ischemia. Brain ischemia is insufficient blood flow to the brain, and can be ... Ischemia or ischaemia is a restriction in blood supply to tissues, causing a shortage of oxygen that is needed for cellular ... Chronic ischemia of the brain may result in a form of dementia called vascular dementia. A brief episode of ischemia affecting ...
"Ischemia-modified albumin reduction after coronary bypass surgery is associated with the cardioprotective efficacy of cold- ... including unpleasant cold allodynia in the distal extremities, mouth, and throat and usually associated with muscle cramps. ...
Location of a transplant center with respect to a donor hospital is given priority due to the effects of Cold Ischemic Time ( ... due to ischemia. Perfusion devices, often called kidney pumps, can extend graft survival to 36-48 hours post recovery for ... Developments in kidney preservation have yielded a device that pumps cold preservation solution through the kidneys vessels to ... ischemia). Each organ tolerates different ischemic times. Hearts and lungs need to be transplanted within 4-6 hours from ...
When weather is cold, ankle is cold, blue and often tender. In hot weather, ankle becomes hot, edematous, swollen and painful. ... Abnormal arterial supply causes low-grade ischemia of ankle region. The ankle skin becomes sensitive to temperature changes. ...
Acute localized ischemia of these structures also may be an important cause. Especially in children, vestibular neuritis may be ... Some people will report having an upper respiratory infection (common cold) or flu prior to the onset of the symptoms of ... 30% of affected people had a common cold prior to developing the disease. Either bacterial or viral labyrinthitis can cause a ... preceded by symptoms of a common cold. However, the causative mechanism remains uncertain. This can also be brought on by ...
Skin biopsies of the site show necrosis caused by ischemia. Radiographic imaging may help to delineate the extent of the wound ... or cold compresses applied to the wound. The diagnosis is mainly clinical. ... Microscopic examination of affected tissue shows ischemic necrosis, and so various hypotheses exist to explain this ischemia, ...
"s of ischemia[citation needed] Pain Pallor (increased) Pulse (decreased) Perishing cold Paraesthesia Paralysis Most commonly, ... It is classically associated with early-stage peripheral artery disease, and can progress to critical limb ischemia unless ...
... among which are ischemia, reperfusion injury, and bacterial colonization. Ischemia is an important factor in the formation and ... Blood vessels constrict in tissue that becomes cold and dilate in warm tissue, altering blood flow to the area. Thus keeping ... Like ischemia, bacterial colonization and infection damage tissue by causing a greater number of neutrophils to enter the wound ... Ischemia results from the dysfunction and, combined with reperfusion injury, causes the tissue damage that leads to the wounds ...
Therefore, cold fluids and foods are encouraged, which facilitate clot formation and prevent its disintegration. Avoiding ... It reduces the blood supply, leading to tissue ischemia, reduced tissue perfusion and eventually higher incidence of painful ...
A healthy tooth will feel the cold but the pain will be mild and disappear once the stimulus is removed. The accuracy of these ... This may lead to ischemia (lack of oxygen) and necrosis (tissue death). Pulpitis is termed reversible when the inflamed pulp is ... Pulpitis is reversible when the pain is mild to moderate and lasts for a short time after a stimulus (for instance cold); or ... Hot drinks can make the tooth feel worse because they expand the gases and likewise, cold can make it feel better, thus some ...
... but without diffuse forefoot ischemia. The development of blue or violaceous toes can also occur with trauma, cold-induced ... Blue toe syndrome is a situation that may reflect atherothrombotic microembolism, causing transient focal ischaemia, ...
In individuals with severe PAD, complications may arise, including critical limb ischemia and gangrene. Critical limb ischemia ... This can lead to pain at rest, feeling of cold, or numbness in the affected foot and toes. Other complications of severe PAD ... 2016), "Critical limb ischemia: current trends and future directions", J Am Heart Assoc, 5 (2): e002938, doi:10.1161/JAHA. ... Wounds are graded 0 through 3 on the presence of ulceration and/or gangrene and ischemia. Grade 0: no ulcer, no gangrene Grade ...
... also has neuroprotective properties in its demonstrated anti-hypoxia or anti-ischemia effects; there is a ... Hot flushes/cold sensation. *Photopsia. *Dyspnoea. *Visual disturbances. *Increased hepatic enzymes without associated clinical ...
... and also reduced severity of colds.[7] A subset of trials reported that supplementation reduced the incidence of colds by half ... "Liposomal-encapsulated Ascorbic Acid: Influence on Vitamin C Bioavailability and Capacity to Protect Against Ischemia- ... the Common Cold". California and Western Medicine. 62 (6): 309-10. PMC 1781017. PMID 18747053.. ... Pauling L (1970). Vitamin C and the Common Cold (1 ed.). San Francisco: W. H. Freeman. ISBN 9780716701590. . Retrieved August ...
Ischemia. *Brain ischemia. *Ischaemic heart disease. *large intestine: Ischemic colitis. *small intestine: Mesenteric ischemia ... Cold intolerance. Acrocyanosis. Erythrocyanosis crurum. Radiation. Radiation poisoning. Radiation burn. Chronic radiation ...
Such features include secondary hypothyroidism with tiredness, intolerance to cold, constipation, weight gain, hair loss and ... and are therefore most likely to be affected by ischemia), all the patients had growth hormone deficiency. Hormonal assay : ...
Ischemia[edit]. Main article: Ischemia. Ischemia, meaning insufficient blood flow to a tissue, can also result in hypoxia. This ... If tissue is not being perfused properly, it may feel cold and appear pale; if severe, hypoxia can result in cyanosis, a blue ...
Any cause of inflammation such as common cold, influenza, and bacterial infections may cause transient vertigo if it involves ... and vasospasm of the blood vessels supplying the labyrinth or central vestibular pathways resulting in ischemia to these ...
New York: Cold Spring Harbor Laboratory Press. ISBN 978-0-87969-888-1. D. Bowen, Ivor (1993). Cell Biology International 17. ... "Overexpression of BCL-2 in transgenic mice protects neurons from naturally occurring cell death and experimental ischemia". ... New York: Cold Spring Harbor Laboratory Press. ISBN 978-0-87969-887-4. Kierszenbaum, Abraham (2012). Histology and Cell Biology ...
Lower than 8 mg pills are still available Otc for sinus, head colds and flu, but is completely illegal in over the counter ... Schier JG, Traub SJ, Hoffman RS, Nelson LS (2003). "Ephedrine-induced cardiac ischemia: exposure confirmed with a serum level ... colds, allergies, other disease, or traditional Asian use.[46] On April 14, 2005, the U.S. District Court for the District of ...
An acute disease is a short-lived disease, like the common cold.. Chronic disease. A chronic disease is one that lasts for a ... Included in this category are contagious diseases-an infection, such as influenza or the common cold, that commonly spreads ... For example, a common cold is a primary disease, where rhinitis is a possible secondary disease, or sequela. A doctor must ... Ischemia. *Inflammation. *Cell damage. *Wound healing. Cellular adaptation. Atrophy. Hypertrophy. Hyperplasia. Dysplasia. ...
Excessive urination and extreme thirst and increased fluid intake (especially for cold water and sometimes ice or ice water) ...
Cold Spring Harb. Symp. Quant. Biol. 73: 403-10. doi:10.1101/sqb.2008.73.018. PMID 19022746.. ... "Therapeutic benefit of intravenous administration of bone marrow stromal cells after cerebral ischemia in rats". Stroke. 32 (4 ...
... and also ischaemia-induced brain injury. In particular, it has been demonstrated to reduce neuronal losses and to improve ...
... and extreme hot or cold temperatures can affect a worker's ability to judge force and strength, which can lead to development ... Ischemia-repurfusion injuries of the appendicular musculoskeletal system. ReferencesEdit. *^ "CDC - NIOSH Program Portfolio : ...
common cold). Head. sinuses. Sinusitis. nose. Rhinitis Vasomotor rhinitis. Atrophic rhinitis. Hay fever. Nasal polyp. ...
This link is "dead cold" - archive.is, WayBack, WebCite, etc.) *^ "La ONT estima en 94.500 los transplantes de órganos solidos ... but failed due to Ischemia. Joseph Murray and J. Hartwell Harrison performed the first successful transplant, a kidney ...
Thermal - hot and cold drinks and foods,[7] cold air, coolant water jet from a dental instrument. ... will irreversibly progress to pulpal necrosis due to compression of the venous microcirculation and tissue ischemia, and ... The pain is sharp and sudden, in response to an external stimulus.[7] The most common trigger is cold,[1] with 75% of people ... Hot or cold food or drinks, and physical pressure are typical triggers in those individuals with teeth sensitivity. ...
... and cold intolerance. 75% develop a deficiency to gonadotropins (LH and FSH), which control the reproductive hormone glands. ...
Hardison RC (Dec 2012). "Evolution of Hemoglobin and Its Genes". Cold Spring Harb Perspect Med. 2 (12): a011627. doi:10.1101/ ... Ischemia-reperfusion injury of the appendicular musculoskeletal system. ReferencesEdit. *^ a b c GRCh38: Ensembl release 89: ...
... and cold water immersion.[1] If more than two such beats are seen, then the condition is termed junctional rhythm. On the ... Active ischemia. *Angina pectoris *Prinzmetal's angina. *Stable angina. *Acute coronary syndrome *Myocardial infarction ...
Usage of a tight, non-elastic bandage will result in reduction of adequate blood flow, potentially causing ischemia. The fit ... "RICE" - Rest, Immobilize, Cold, Elevate[10]. *"HI-RICE" - Hydration, Ibuprofen, Rest, Ice, Compression, and Elevation ... To prevent localised ischemia or frostbite to the skin, it is recommended that the ice be placed within a towel or other ...
Ischaemia *Avascular necrosis. *Osteonecrosis of the jaw. *Algoneurodystrophy. *Hypertrophic pulmonary osteoarthropathy. * ...
There is no official or formal dividing line between hypoperfusion and ischemia; sometimes the latter term refers to zero ... Poor perfusion (malperfusion), that is, ischemia, causes health problems, as seen in cardiovascular disease, including coronary ... Cold shock response. *Dead space (physiology). *Decompression (diving). *Decompression theory. *Diving reflex ...
"New Orleans filmmaker cleared in cold-case murder; false positive highlights limitations of familial DNA searching". The New ... Variable degrees of hemolysis and intermittent episodes of vascular occlusion resulting in tissue ischemia and acute and ...
Algra A; Halkes, PH; Van Gijn, J [et al] «Medium intensity oral anticoagulants versus aspirin after cerebral ischaemia of ... Stroke associated with sympathomimetics contained in over-the-counter cough and cold drugs». Stroke, 34, 7, 2003, pàg. 1667-72 ... Aspirin plus dipyridamole versus aspirin alone after cerebral ischaemia of arterial origin (ESPRIT): randomised controlled ...
Cerebral ischemia is a frequently disputed possible cause, at least for some segment of the TGA population, and until the 1990s ... The most commonly cited precipitating events include vigorous exercise (including sexual intercourse), swimming in cold water ... One current hypothesis is that TGA may be due to venous congestion of the brain, leading to ischemia of structures involved ...
common cold). Head. sinuses. Sinusitis. nose. Rhinitis Vasomotor rhinitis. Atrophic rhinitis. Hay fever. Nasal polyp. ... Ischemia. *Brain ischemia. *Ischaemic heart disease. *large intestine: Ischemic colitis. *small intestine: Mesenteric ischemia ...
... and individuals who immigrate from warm climates to cold climates, especially women who wear traditional veils or dresses that ... Ischaemia *Avascular necrosis. *Osteonecrosis of the jaw. *Algoneurodystrophy. *Hypertrophic pulmonary osteoarthropathy. * ...
March 1961). "Cerebral ischemia and infarction". Am Pract Dig Treat. 12: 147-54. PMID 13777430.. CS1 maint: Uses authors ... Cold shock response. *Dead space (physiology). *Decompression (diving). *Decompression theory. *Diving reflex ...
The use of these organs could be increased if "cold ischemia times" are limited. Donated organs that could be used to save ... The process of restoring blood flow following this period of "cold ischemia" can lead to tissue damage. To examine the ... Kidney donation after circulatory death has become common in the U.S and study suggests that limiting cold ischemia time to ... Ice cream headache or brain freeze is a temporary pain due to eating or drinking a cold substance or when exposed to cold air. ...
... ... Nathalie Le Clef, Anja Verhulst, Patrick C. DHaese, and Benjamin A. Vervaet, "Unilateral Renal Ischemia-Reperfusion as a ... "Effect of Γ-aminobutyric acid on kidney injury induced by renal ischemia-reperfusion in male and female rats: Gender-related ...
i,Results,/i,. Prolonged cold ischemia time in UW solution increased PARP-1 expression and kidney injury. Preconditioning with ... In conclusion, PARP inhibition attenuates ATN and other IR-related renal lesions in mouse kidneys under prolonged cold storage ... If confirmed, these data suggest that pharmacological manipulation of PARP activity may have salutary effects in cold-stored ... and other renal lesions related to prolonged cold ischemia/reperfusion (IR) in kidneys preserved at 4°C in University of ...
... the pathophysiology of liver I/R and regeneration making clear distinction between situations of cold and warm ischemia, as ... Ischemia-reperfusion (I/R) associated with hepatic resection and living related liver transplantation is an unsolved problem in ... type of ischemia applied (warm or cold), period of ischemia (ranging from minutes to days), extension of hepatic ischemia ( ... 4. Controversies on hepatic regeneration under warm or cold ischemia. In an attempt to expand the size of the donor pool, the ...
Hearts, saphenous veins, internal-mammary-arteries and aortas of male landrace pigs were harvested and exposed to cold ischemia ... HTK-Custodiol protection of mitochondrial function after prolonged cold ischemia was observed in the myocardium but not in ... mitochondrial function remained stable even after 24 h of cold ischemia. ... Analysis of mitochondrial function can be used as a valuable approach for the assessment of cold ischemic injury in various ...
Cold Ischemia During Surgery. Known as: cold ischemia In surgery, the cooling of a tissue, organ, or body part after its blood ... Laparoscopic partial nephrectomy in cold ischemia: renal artery perfusion.. *Gunter Janetschek, Alaa Eldin A. Abdelmaksoud, Dr ... We examined the effects of upregulation of heme oxygenase-1 (HO-1) in steatotic rat liver models of ex vivo cold ischemia… ( ... BACKGROUND Prolonged cold ischemia time (CIT) is accompanied by delayed cadaveric renal allograft function and early allograft ...
CitacióFerreira, P., Muñoz, M., Reverter Comes, F. The effects of death and post-mortem cold ischemia on human tissue ... The effects of death and post-mortem cold ischemia on human tissue transcriptomes. ...
The cold ischemia time (CIT) was recorded. Statistical comparisons were made among HA level, CK-BB level, CIT, and graft ... CAN EFFLUENT HYALURONIC ACID OR CREATINE KINASE PREDICT SINUSOIDAL INJURY SEVERITY AFTER COLD ISCHEMIA?. ...
... and cold (4°C) ischemia on different mitochondrial functions in rat brain, liver ... Baumann, M., Bender, E., Sömmer, G. et al. Effects of warm and cold ischemia on mitochondrial functions in brain, liver and ... Effects of warm and cold ischemia on mitochondrial functions in brain, liver and kidney. *Matthias Baumann1. , ... The purpose of this work was to study the effects of warm (37°C) and cold (4°C) ischemia on different mitochondrial functions ...
... Roberto Troisi (UGent) , D ... Troisi R, MEESTER D, DE RYCK F, Cuvelier C, HESSE U. The impact of prolonged cold ischemia on delayed graft function (DGF) in ... R. Troisi, D. MEESTER, F. DE RYCK, C. Cuvelier, and U. HESSE, "The impact of prolonged cold ischemia on delayed graft function ... Troisi, R., MEESTER, D., DE RYCK, F., Cuvelier, C., & HESSE, U. (1999). The impact of prolonged cold ischemia on delayed graft ...
However, the time of cold ischemia still has an important influence on DGF (8). Cold storage and reperfusion both cause loss of ... and protection against cold ischemia/reperfusion. The NMR spectroscopy data indicate that cold preservation of the kidneys with ... Takada M, Chandraker A, Nadeau KC, Sayegh MH, Tilney NL: The role of B7 costimulatory pathway in experimental cold ischemia/ ... Free radicals and other reactive oxygen species that trigger T cell activation (1) are produced after cold ischemia and ...
Prevention of primary graft dysfunction in lung transplantation by N-acetylcysteine after prolonged cold ischemia ... Download PDF Prevention of primary graft dysfunction in lung transplantation by N-acetylcysteine after prolonged cold ischemia ... Prevention of primary graft dysfunction in lung transplantation by N-acetylcysteine after prolonged cold ischemia. Journal of ... and recipient treatment with NAC effectively protected the lung from primary graft dysfunction after prolonged cold ischemia. ...
Objective The purpose of this study is to describe the functional relationship between cold-ischemia time (CIT) and primary ...
The effect of oxygenated warm buffer perfusion with PGE1 before cold preservation on hepatic warm ischemia reperfusion injury. ... Journal Article] Perfusion Using Oxygenated Buffer Containing Prostaglandin E1 before Cold Preservation Prevents Warm Ischemia- ... Presentation] Perfusion by oxygenated buffer containing prostaglandin E1 prior to cold preservation prevents warm ischemia- ... Presentation] Perfusion by oxygenated buffer containing prostaglandin E1 prior to cold preservation prevents warm ischemia- ...
The concept of time may be discussed on several different... Explanation of cold ischemia time ... Find out information about cold ischemia time. sequential arrangement of all events, or the interval between two events in such ... redirected from cold ischemia time). Also found in: Dictionary, Thesaurus, Medical, Legal.. Related to cold ischemia time: warm ... Cold ischemia time , Article about cold ischemia time by The Free Dictionary https://encyclopedia2.thefreedictionary.com/cold+ ...
Researchers have long considered the NCI supported Cooperative Human Tissue Network (CHTN) as a source for quality collection and preservation of tissue samples from remnant surgical tissue specimens. In an organized tissue procurement setting serving a large population of researchers, the trained procurement personnel, specialized reagents and monitoried frozen storage facilities assure the availability of quality research, monitored preservation techniques and temperature controlled shipping required for successful research support.. The CHTN Midwestern Division (MWD) has experienced an increase in investigator demand for fresh tissues over the past two years. Approximately 23% of the tissues distributed by the CHTN MWD is fresh tissue. Requests for frozen and formlin-fixed paraffin embedded tissues, however, remain constant and stable. The increased interest in fresh tissue likely reflects an increased focus on the study of vital cells in controlled environments for... Read more ...
"Cold Ischemia" by people in UAMS Profiles by year, and whether "Cold Ischemia" was a major or minor topic of these publications ... Cold ischemia time during ORGAN TRANSPLANTATION begins when the organ is cooled with a cold perfusion solution after ORGAN ... "Cold Ischemia" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical Subject ... Below are the most recent publications written about "Cold Ischemia" by people in Profiles over the past ten years. ...
Digital Ischemia. 14/07/2017. Cold Call: Day 1 Filed under: Cold Call-R - Tags: audioplay, creative writing, dialogue, fiction ... Leave a Reply to Cold Call: Day 15 , Digital Ischemia Cancel reply. Enter your comment here.... ... series of telephone conversations where truth comes away in layers - begins at Cold Call: Day 1 and ends at Day […] ... series of telephone conversations where truth comes away in layers - begins at Cold Call: Day 1 and ends at Day […] ...
... begins at Cold Call: Day 1 SOUND: PHONE RINGS LACRIMOSA: Still speaking to me? SìMON: (D) Ah, I suddenly thought oh shit, ... Digital Ischemia. 27/07/2017. Cold Call: Day 14 Filed under: Cold Call-R - Tags: comedy, creative writing, dialogue, drama, ... A series of telephone conversations where truth comes away in layers - begins at Cold Call: Day 1 ...
In renal transplantation, cold ischaemia (CI) determines acute rejection through innate immunity among others. Acute rejection ... In renal transplantation, cold ischaemia (CI) determines acute rejection through innate immunity among others. Acute rejection ...
... ... Conclusions IL-6/STAT3 pathway might participate to initiate cholangiecytes regeneration after cold ischemia and preservation ... Objective To investigate the role of IL-6/STAT3 pathway in the proliferation of cholangiocyte induced by cold ischemia and ... Cholangiocytes responded to extended cold preservation with severe bile duct injures and marked increase in IL-6 secretion, p- ...
Experts available for cold ischaemia consulting, expert witness, or analyst services for business, legal, and technical ... cold ischaemia Experts. To improve your results, click a Related Expert Area on the left.. ... Join our distinguished experts for cold ischaemia jobs, projects and consulting opportunities. ...
OKT3 prophylaxis in Renal Grafts with prolonged cold ischemia times: association with improvement in long term survival. ... OKT3 prophylaxis in Renal Grafts with prolonged cold ischemia times: association with improvement in long term survival ...
Clinicians may be reluctant to transplant small pediatric kidneys that have prolonged cold ischemia time (CIT) for fear of an ... Influence of Cold Ischemia Time in Kidney Transplants From Small Pediatric Donors. ... additional deleterious effect because pediatric grafts are thought to be more sensitive to ischemia. We aimed to assess the ...
Effects of Cold Ischemia on Tissue Oxygenation. Additionally, mean oxy-hemoglobin concentrations were compared for cold and ... Sequential oxygenation images of a left kidney at baseline (a) and during cold ischemia (b-h) and reperfusion (i-o). Ischemia ... decreases with ischemia time, and the kidney is darkest after 30 min of ischemia, demonstrating significantly lowered %. HbO. 2 ... B. R. Laneet al., "Comparison of cold and warm ischemia during partial nephrectomy in 660 solitary kidneys reveals predominant ...
The need to reduce cold ischemia time in kidney transplantation. van der Vliet, J. Adam; Warlé, Michiel C. ... Preservation solutions for static cold storage of abdominal allografts: which is best?. Parsons, Ronald F.; Guarrera, James V. ... Role of temperature in reconditioning and evaluation of cold preserved kidney and liver grafts. Minor, Thomas; von Horn, ... Donor risk factors, retrieval technique, preservation and ischemia/reperfusion injury in pancreas transplantation. Maglione, ...
Influence of Pancreas Import and Cold Ischemia on Outcomes Following Pancreas Transplantation.. M. Khan,1 H. El-Hennawy,1 J. ... Influence of Cold Ischemia Time in Combination With Donor Ischemic Events on Kidney Transplant Outcomes. ... Proposed changes in pancreas transplant (PT) allocation may improve organ sharing but increase cold ischemia time (CIT). ... Influence of Pancreas Import and Cold Ischemia on Outcomes Following Pancreas Transplantation. Am J Transplant. 2016;16 (suppl ...
Cold Ischemia. The common definition for cold ischemia in surgery is the time between the chilling of a tissue, organ, or body ... The effects of warm and cold ischemia can be classified into ischemia-induced metabolic responses including posttranslational ... Effects of cold ischemia on gene expression: a review and commentary. Biopreserv Biobank. 2016;14(6):548-58.CrossRefPubMed ... Cold ischemia triggers a cascade of noxious effects and responses to hypoxia and stress, all of which affect the quality of the ...
Phenotype and Course of Renal Tubular Cell Death during Cold Ischemia versus Cold Ischemia Followed by Transplant. ... Cellular Sources of Cytokine Production during Cold Ischemia Induced AKI after Kidney Transplant. S. Jain1, R. Plenter2, T. ... Purpose: Delayed graft function portends reduced 5-year graft survival and is associated with prolonged cold ischemia (CI). The ... Jain S, Plenter R, Nydam T, Jani A. Cellular Sources of Cytokine Production during Cold Ischemia Induced AKI after Kidney ...
Blood; Cold Ischemia; Death; Female; gene expression; Humans; Models, Biological; Postmortem Changes; RNA, Messenger; ... The effects of death and post-mortem cold ischemia on human tissue transcriptomes.. ... The effects of death and post-mortem cold ischemia on human tissue transcriptomes.. ...
  • Marzieh Vafapour, Mehdi Nematbakhsh, Ramesh Monajemi, Safoora Mazaheri, Ardeshir Talebi, Nahid Talebi, and Soheyla Shirdavani, "Effect of Γ-aminobutyric acid on kidney injury induced by renal ischemia-reperfusion in male and female rats: Gender-related difference. (hindawi.com)
  • We test the hypothesis that PARP inhibition can decrease acute tubular necrosis (ATN) and other renal lesions related to prolonged cold ischemia/reperfusion (IR) in kidneys preserved at 4°C in University of Wisconsin (UW) solution. (hindawi.com)
  • Until now, renal ischemia-reperfusion (IR) injury has been inevitable in transplantation, resulting in the cell death of renal tubular epithelial cells. (hindawi.com)
  • Ischemia-reperfusion (I/R) associated with hepatic resection and living related liver transplantation is an unsolved problem in clinical practice. (intechopen.com)
  • In most cases, in clinical practice, liver surgery involves both ischemia-reperfusion (I/R) injury and regeneration [ 1 ]. (intechopen.com)
  • Amelioration of rat cardiac cold ischemia/reperfusion injury with inhaled hydrogen or carbon monoxide, or both. (semanticscholar.org)
  • H-NMR-based metabolic signatures of mild and severe ischemia/reperfusion injury in rat kidney transplants. (semanticscholar.org)
  • BACKGROUND Severe ischemia/reperfusion (IR) injury is a risk factor for delayed graft function. (semanticscholar.org)
  • Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. (semanticscholar.org)
  • Mitochondria play a critical role in myocardial cold ischemia-reperfusion (CIR) and induction of apoptosis. (semanticscholar.org)
  • Heme oxygenase-1 overexpression protects rat hearts from cold ischemia/reperfusion injury via an antiapoptotic pathway. (semanticscholar.org)
  • BACKGROUND Ischemia/reperfusion (I/R) injury is one of the most important causes of the early graft loss. (semanticscholar.org)
  • Upregulation of heme oxygenase-1 protects genetically fat Zucker rat livers from ischemia/reperfusion injury. (semanticscholar.org)
  • Ischemia/reperfusion injury leads to delayed graft function, which is a major problem in kidney transplantation. (asnjournals.org)
  • These results indicate that the tissue damage due to ischemia/reperfusion injury may be prevented, at least in part, by adding TMZ to preservation solutions. (asnjournals.org)
  • The renal damage resulting from ischemia and reperfusion may have adverse effects on the outcome of organ transplants, increasing both posttransplant morbidity and the early loss of transplanted kidneys. (asnjournals.org)
  • Cold storage and reperfusion both cause loss of cell polarity, disruption of the cytoskeleton, and perturbations in the polarized membrane transport proteins in postischemic kidneys ( 9 , 10 ). (asnjournals.org)
  • Mononuclear cells (T cells and monocyte/macrophages) infiltrate postischemic rat kidneys, and the expression of T cell-associated cytokines, monocyte/macrophage activation products, and MHC class II antigen are upregulated after cold ischemia/reperfusion injury ( 11 , 12 ). (asnjournals.org)
  • However, the early and late consequences of ischemia/reperfusion injury can be prevented by soluble P- and E-selectin ligand ( 11 ), or by blocking T cell costimulatory activation ( 12 ) in the absence of alloantigen. (asnjournals.org)
  • This drug impairs lipid peroxidation and reduces intracellular acidosis during the cold storage and reperfusion of isolated rat kidneys ( 15 ). (asnjournals.org)
  • TMZ also protects the functions of mitochondria from rat liver subjected to ischemia (30 min) and reperfusion ( 16 ). (asnjournals.org)
  • In this study, we perfused rat livers from DCD before cold preservation with oxygenated warm buffer containing Prostaglandin E1 and observed bile production, liver cell death, mitochondrial function of liver cell and so on during reperfusion with oxygenated buffer. (nii.ac.jp)
  • Presentation] Perfusion by oxygenated buffer containing prostaglandin E1 prior to cold preservation prevents warm ischemia-reperfusion injury in liver grafts from non-heart-beating donors. (nii.ac.jp)
  • Objective To investigate the role of IL-6/STAT3 pathway in the proliferation of cholangiocyte induced by cold ischemia and reperfusion injury. (oriprobe.com)
  • To better understand the dynamics of intraoperative renal ischemia and recovery of renal oxygenation during reperfusion, a visible reflectance imaging system (VRIS) was developed to measure renal oxygenation during renal artery clamping in both cooled and warm porcine kidneys. (spiedigitallibrary.org)
  • Cold ischemia/reperfusion injury of the hepatic graft has been attributed to the release of various inflammatory cytokines. (elsevier.com)
  • Decoy ODNs against nuclear factor-κB binding site delivery improved the viability of the hepatic graft against cold ischemia/reperfusion injury in the rodent liver transplantation model. (elsevier.com)
  • The extent of graft damage after ischemia-reperfusion reflects the balance between deleterious events and protective factors. (asnjournals.org)
  • For investigating whether HO-1 and VEGF play a role in the adaptive response to ischemia-reperfusion injury after renal transplantation, kidney biopsies were analyzed from living ( n = 45) and cadaveric ( n = 16) donors, obtained at three time points: at the end of cold storage T(−1), after warm ischemia T(0), and after reperfusion T(+1). (asnjournals.org)
  • It is concluded that the lower expression of HO-1, VEGF 165 , and Bcl-2 in cadaveric donor kidneys can reflect a defective adaptation against ischemia-reperfusion injury that may affect their function in the short term. (asnjournals.org)
  • An appropriate protective response may tip the balance toward a less hazardous event related to the damage of ischemia-reperfusion process. (asnjournals.org)
  • Acute ischemia - reperfusion (I/R) of extremities means serious challenge in the clinical practice. (bvsalud.org)
  • Before operations, from the excluded limb by the end of ischemia , during the reperfusion , and for 5 postoperative days afterwards blood samples were collected for testing hematological and blood coagulation parameters . (bvsalud.org)
  • Cold storage (CS) remains the gold standard for organ preservation worldwide, although it is inevitably associated with ischemia/reperfusion injury (IRI). (fujita-hu.ac.jp)
  • The fragility of the lungs limits the procurement rate and can favor the occurrence of ischemia-reperfusion injury (IRI). (elsevier.com)
  • Numerous pharmacological interventions aiming to prevent ischemia-reperfusion injuries failed to reduce the rate of DGF. (springer.com)
  • During the transplantation procedure, the kidneys suffer from warm (harvesting and grafting before vascular anastomosis) and cold ischemia (preservation) followed by reperfusion. (springer.com)
  • The association of hypoxic and ischemia-reperfusion injuries decreases renal blood flow, increases oxidative stress and triggers the release of pro-inflammatory mediators which activate leukocyte adhesion and immune phenomena. (springer.com)
  • Mitochondria play a central role in the pathophysiology of ischemia-reperfusion injuries. (springer.com)
  • Here we demonstrate that in contrast to the proinflammatory role of HMGB1, preconditioning with HMGB1 results in protection following hepatic ischemia/reperfusion (I/R). Pretreatment of mice with HMGB1 significantly decreased liver damage after I/R. The protection observed in mice pretreated with HMGB1 was associated with a higher expression of IL-1R-associated kinase-M, a negative regulator of TLR4 signaling, compared with controls. (jimmunol.org)
  • Ischemia/reperfusion (I/R) 4 injury is a pathophysiologic process whereby hypoxic organ damage is accentuated following return of blood flow and oxygen delivery. (jimmunol.org)
  • HMGB1 levels are increased by ischemia/reperfusion in the liver and activation of the innate immune system by HMGB1 in this context requires TLR4-dependent signaling. (jimmunol.org)
  • In kidney transplantation, injury due to ischemia and reperfusion is an inevitable process resulting many times in delayed graft function (DGF) that is currently characterized by the need of dialysis within the first week after transplantation. (scielo.br)
  • Ischemia and reperfusion injury (IRI) also promotes activation of the innate and adaptive responses of the immune system, leading to processes with great potential to produce significant graft harm. (scielo.br)
  • To elucidate the role of DAP12 in pulmonary ischemia/reperfusion injury (IRI), we took advantage of a clinically relevant mouse model of transplant-mediated lung IRI. (jimmunol.org)
  • Ischemia/reperfusion injury (IRI) is a clinically relevant noninfectious inflammatory insult that can affect a variety of tissues. (jimmunol.org)
  • In the case of lung transplantation, where reperfusion of a lung transiently subjected to cold ischemia is an inherent component of this procedure, IRI is a critical determinant of short- and long-term morbidity and mortality ( 3 ). (jimmunol.org)
  • He is interested in the pathogenesis of kidney ischemia-reperfusion injury and transplant rejection, and molecular control of urinary malignancies. (vchri.ca)
  • Reduction of cold ischemia-reperfusion injury by graft-expressing clusterin in heart transplantation. (vchri.ca)
  • Expression of transforming growth factor-beta1 limits renal ischemia-reperfusion injury. (vchri.ca)
  • Loss of clusterin expression worsens renal ischemia-reperfusion injury. (vchri.ca)
  • Among abdominal solid organ transplantation, pancreatic grafts are prone to develop severe ischemia reperfusion injury-associated graft damage, leading eventually to early graft loss. (jove.com)
  • The chronological expression (over 24 h) of two adhesion molecules [intercellular adhesion molecule-1 (ICAM-1) and CD11b/CD18 (Mac-1)] and the extent of liver damage, including injury to sinusoidal endothelial cells (SECs) and hepatocyte apoptosis, were investigated under two conditions of rat liver ischemia-reperfusion (I/R) injury: reversible (30 min) and fatal I/R (60 min). (physiology.org)
  • hepatic injury induced by ischemia-reperfusion (I/R) is an important clinical problem that often follows circulatory shock with resuscitation, as well as many types of liver surgery, including transplantation and tumor resection. (physiology.org)
  • Rationale Primary graft dysfunction in lung transplant recipients derives from the initial, largely leukocyte-dependent, ischaemia-reperfusion injury. (bmj.com)
  • Intravascular lung-marginated monocytes have been shown to play key roles in experimental acute lung injury, but their contribution to lung ischaemia-reperfusion injury post transplantation is unknown. (bmj.com)
  • Methods Isolated perfused C57BL/6 murine lungs were subjected to warm ischaemia (2 hours) and reperfusion (2 hours) under normoxic conditions. (bmj.com)
  • Results In mouse lungs following ischaemia-reperfusion, substantial numbers of lung-marginated monocytes remained within the pulmonary microvasculature, with reduced L-selectin and increased CD86 expression indicating their activation. (bmj.com)
  • Conclusions These results indicate that lung-marginated intravascular monocytes are retained as a 'passenger' leukocyte population during lung transplantation, and play a key role in the development of transplant-associated ischaemia-reperfusion injury. (bmj.com)
  • Resident lung mononuclear phagocytes have been implicated in ischaemia-reperfusion injury and the development of primary graft dysfunction following lung transplantation, but the contribution of lung intravascular monocytes to lung transplant-related injury is not known. (bmj.com)
  • This study suggests that vascular monocytes play a key role in lung injury following ischaemia-reperfusion. (bmj.com)
  • Graft cellular death was an early event detected at H3 and seems to be one of the first ischemia reperfusion injuries. (biomedcentral.com)
  • Donor deceased after cardiac death (DCD), is an additional potential source of kidney graft which is more prone to severe ischemia reperfusion injury (IRI), primary non-function (PNF), and delayed graft function (DGF). (biomedcentral.com)
  • Kidney donation after circulatory death has become common in the U.S and study suggests that limiting cold ischemia time to less than 12 hours can reduce kidney wastage. (medindia.net)
  • If confirmed, these data suggest that pharmacological manipulation of PARP activity may have salutary effects in cold-stored organs at transplantation. (hindawi.com)
  • Acute tubular necrosis (ATN) induced by IR injury contributes to the development of delayed graft function (DGF), which remains an important complication of kidney transplantation and, despite advances in cold storage, is a major risk factor for acute rejection and long-term kidney allograft dysfunction [ 3 ]. (hindawi.com)
  • The impact of prolonged cold ischemia on delayed graft function (DGF) in pancreas transplantation. (ugent.be)
  • Troisi R, MEESTER D, DE RYCK F, Cuvelier C, HESSE U. The impact of prolonged cold ischemia on delayed graft function (DGF) in pancreas transplantation. (ugent.be)
  • Improvements in cold preservation techniques and solutions, such as the University of Wisconsin (UW) solution, have greatly facilitated the use of organs, including kidneys, for transplantation. (asnjournals.org)
  • METHODS: Porcine single left-lung transplantation was performed in 2 experimental groups after 24 hours of cold storage. (uzh.ch)
  • The liver grafts which are taken from the donors after cardiac death(DCD)and preserved immediately in cold solution show bad function after transplantation. (nii.ac.jp)
  • Cold ischemia time during ORGAN TRANSPLANTATION begins when the organ is cooled with a cold perfusion solution after ORGAN PROCUREMENT surgery, and ends after the tissue reaches physiological temperature during implantation procedures. (uams.edu)
  • Lo S, MacMillan-Crow LA, Parajuli N. Renal cold storage followed by transplantation impairs proteasome function and mitochondrial protein homeostasis. (uams.edu)
  • In renal transplantation, cold ischaemia (CI) determines acute rejection through innate immunity among others. (oup.com)
  • Conclusions IL-6/STAT3 pathway might participate to initiate cholangiecytes regeneration after cold ischemia and preservation injury,which might benefit biliary recovery after fiver transplantation. (oriprobe.com)
  • Renal ischemia that occurs intraoperatively during procedures requiring clamping of the renal artery (such as renal procurement for transplantation and partial nephrectomy for renal cancer) is known to have a significant impact on the viability of that kidney. (spiedigitallibrary.org)
  • Influence of Pancreas Import and Cold Ischemia on Outcomes Following Pancreas Transplantation. (atcmeetingabstracts.com)
  • Khan M, El-Hennawy H, Rogers J, Farney A, Orlando G, Reeves-Daniel A, Palanisamy A, Stratta R. Influence of Pancreas Import and Cold Ischemia on Outcomes Following Pancreas Transplantation. (atcmeetingabstracts.com)
  • Cold ischemia time and allograft outcomes in live donor renal transplantation: Is live donor organ transport feasible? (uab.edu)
  • While transport of the kidney is an attractive alternative, there is concern that prolonged cold ischemia time (CIT) would diminish the benefits of live donor transplantation (LDTx). (uab.edu)
  • In rat liver transplantation, the addition of VEGF to cultured sinusoidal endothelial cells attenuated the damage induced by cold preservation, inhibiting cellular apoptosis ( 17 ), which is possibly mediated by the increased expression of the antiapoptotic proteins such as A1 and Bcl-2 ( 18 ). (asnjournals.org)
  • Transient episodes of ischemia are encountered during solid organ transplantation, trauma, hypovolemic shock, and elective liver resection, when inflow occlusion or total vascular exclusion is used to minimize blood loss. (jimmunol.org)
  • This large international trial could be the defining work towards establishing ex-vivo heart perfusion as a viable clinical option for donor heart preservation for transplantation to overcome the challenges of cold ischemic storage,' said Dr. Waleed Hassanein , President and CEO of TransMedics, Inc. (prnewswire.com)
  • Since the advent of organ transplantation, the cornerstone of organ preservation has been cold ischemic storage (essentially placing organs on ice). (prnewswire.com)
  • Partly because of the limitations of cold storage, it is estimated that globally 70% to 80% of donor hearts cannot ultimately be used for transplantation. (prnewswire.com)
  • TransMedics has developed the world's only portable medical device capable of overcoming the limitations of cold storage for organ transplantation. (prnewswire.com)
  • The injury an organ sustains during recovery, preservation, and transplantation occurs primarily as a result of ischemia and hypothermia. (medscape.com)
  • The second, the cold ischemic phase, occurs when the organ is preserved in a hypothermic state prior to transplantation into the recipient. (medscape.com)
  • Deciphering the respective role of renal warm and cold ischemia is of pivotal interest in the transplantation process. (biomedcentral.com)
  • Using a preclinical pig model of renal auto-transplantation, we investigated the consequences of warm and cold ischemia on early innate and adaptive responses as well as graft outcome. (biomedcentral.com)
  • Delayed graft function portends reduced 5-year graft survival and is associated with prolonged cold ischemia (CI). (atcmeetingabstracts.com)
  • Currently, the most effective preservation strategy to reduce ischemic injury during prolonged cold storage is to decrease renal oxygen demand by using both hypothermia and preservation solutions (University of Wisconsin (UW) solution) together. (hindawi.com)
  • Organ preservation solutions play an important role in the prevention of DGF, especially in organs from expanded criteria donors or exposed to prolonged cold ischemia. (hindawi.com)
  • Cholangiocytes responded to extended cold preservation with severe bile duct injures and marked increase in IL-6 secretion, p-STAT3 and cyclinDl protein expression, followed by compensatory cholangiocytes regeneration. (oriprobe.com)
  • ABSTRACT Liquid perfluorocarbon (PFC) instillation has been studied experimentally as an adjuvant therapy in the preservation of lung grafts during cold ischemia . (bvsalud.org)
  • Thirty minutes after cardiac arrest, in situ anterograde selective pulmonary cold perfusion Show more The aim of this study was to assess the functional preservation of the lung graft with anterograde lung perfusion in a model of donation after cardiac death. (univoak.eu)
  • [ 3 ] Organ ischemia and preservation disrupt all these relations. (medscape.com)
  • Decrease in donor heart injury by recombinant clusterin protein in cold preservation with University of Wisconsin solution. (vchri.ca)
  • In such donors, different ischemic conditions are associated such as warm ischemia (WI) and cold ischemia (CS) characterized respectively by an ischemic period at body temperature initiated by cardiac arrest ensuing a low and/or no blood flow and by a hypothermic preservation of kidneys. (biomedcentral.com)
  • Certain DCD kidneys were at higher risk of failing when compared with kidneys from brain dead donors, but only when the cold ischemia time was >12 hours. (medindia.net)
  • Prolonged cold ischemia time in UW solution increased PARP-1 expression and kidney injury. (hindawi.com)
  • The impact of ischemia time during open nephron sparing surgery on solitary kidneys: a multi-institutional study. (semanticscholar.org)
  • The cold ischemia time (CIT) was recorded. (ovid.com)
  • However, the time of cold ischemia still has an important influence on DGF ( 8 ). (asnjournals.org)
  • 5 Two major modifiable intraoperative variables known to affect renal function during ischemia are the length of ischemic time and the temperature of the kidney at which ischemia occurred. (spiedigitallibrary.org)
  • Clinicians may be reluctant to transplant small pediatric kidneys that have prolonged cold ischemia time (CIT) for fear of an additional deleterious effect because pediatric grafts are thought to be more sensitive to ischemia. (ovid.com)
  • Proposed changes in pancreas transplant (PT) allocation may improve organ sharing but increase cold ischemia time (CIT). (atcmeetingabstracts.com)
  • The highest values were on the 2nd day in cold I/R group, accompanied by increased prothrombin time values. (bvsalud.org)
  • Adenosine triphosphate (ATP) depletion during cold ischemia time (CIT) is considered crucial for graft function. (unipd.it)
  • 1 h, however, little has been published about cold ischemia time and its significance in ERBB2 testing. (houstonmethodist.org)
  • To that end, this study evaluated ERBB2 status using two different FDA-approved in-situ hybridization methods and an FDA-approved immunohistochemistry (IHC) assay in the largest cohort to date (n=84) of invasive breast carcinomas with tracked cold ischemia time. (houstonmethodist.org)
  • Fluorescence in-situ hybridization (FISH) and INFORM HER2 ® demonstrated 100% concordance in the detection of ERBB2 amplification by all three scoring guidelines at all cold ischemia time points. (houstonmethodist.org)
  • In addition, Inform HER2 ® in-situ hybridization signals were significantly more intense than FISH at all cold ischemia time points, however, no significant loss of either chromosome 17 or ERBB2 signal was detected by FISH or Inform HER2 ® in-situ hybridization in cold ischemia times up to 3 h. (houstonmethodist.org)
  • On the basis of our findings, cold ischemia time up to 3 h has no deleterious effect on the detection of ERBB2 via in-situ hybridization or IHC. (houstonmethodist.org)
  • The mean cold ischemia time was 31.4 +/- 8.4 hr. (nih.gov)
  • Cold ischemia time greater than 40 hours. (clinicaltrials.gov)
  • For example, livers from African American and female donors were associated with significantly higher risk of graft failure, as were livers with cold ischemia time longer than 8.8 hours. (medpagetoday.com)
  • Although all the variables included in the model are important, donor age, cold ischemia time, recipient MELD score, and cause of liver disease have the greatest impact on survival. (medpagetoday.com)
  • In treating acute limb ischaemia time is everything. (wikipedia.org)
  • The warm ischemia time was 5 minutes. (urofrance.org)
  • The cold ischemia time was 58 minutes. (urofrance.org)
  • cold ischemia time the time between the placement of a traumatically amputated body part in ice and the time of surgical replantation. (thefreedictionary.com)
  • warm ischemia time the time interval between traumatic amputation of a limb or part and its placement on ice. (thefreedictionary.com)
  • Myocardial ischemia can develop slowly as arteries become blocked over time. (mayoclinic.org)
  • We achieved a favorable osteosynthesis, a functional cascade, and perfusion of the allograft in 5.5 hours of cold ischemia time. (aaps1921.org)
  • For liver transplants, the cold ischemia time can be up to 24 hours, although typically surgeons aim for a much shorter period of time. (wikipedia.org)
  • For kidney transplants, as the cold ischemia time increases, the risk of delayed function of the kidney increases. (wikipedia.org)
  • We found that use of DCD kidneys is variable throughout the United States, that the outcomes are generally excellent, and that the use of these organs could probably be safely increased if cold ischemia times are limited," said Dr. Gill. (medindia.net)
  • In conclusion, PARP inhibition attenuates ATN and other IR-related renal lesions in mouse kidneys under prolonged cold storage in UW solution. (hindawi.com)
  • This study investigated the effects of adding trimetazidine (TMZ) to the perfusate of cold-stored kidneys on the function of reperfused autotransplanted pig kidney. (asnjournals.org)
  • The left kidney was removed and cold-flushed with Euro-Collins (EC), or University of Wisconsin (UW) solutions with or without 10 -6 M TMZ and stored for 48 h at 4°C. The kidneys were then autotransplanted and the contralateral kidneys were removed. (asnjournals.org)
  • it was 100% for pigs having kidneys cold-flushed with TMZ-supplemented UW and EC solutions. (asnjournals.org)
  • The functions of the transplanted kidneys were also better preserved after cold flush with TMZ-supplemented solutions than with TMZ-free solutions. (asnjournals.org)
  • Creatinine clearance was higher and the urinary excretion of trimethylamine- N -oxide and dimethylamine, used as markers of renal medulla injury, were lower in animals transplanted with kidneys cold-flushed with TMZ-supplemented solutions than with TMZ-free solutions. (asnjournals.org)
  • The anti-ischemic drug trimetazidine (TMZ) has been shown to improve the function of isolated perfused pig kidneys exposed to prolonged cold ischemia ( 14 ). (asnjournals.org)
  • Mean relative oxy-hemoglobin concentrations decrease more significantly during ischemia for normothermic kidneys compared to hypothermic kidneys. (spiedigitallibrary.org)
  • 13% of the cadaveric kidneys were from pediatric donors less than 3 years of age and were transplanted en bloc. (nih.gov)
  • Patients who are recipients of multiple solid organ transplants, including dual and en bloc kidneys, or who have previously received transplanted organs. (clinicaltrials.gov)
  • Kidneys were subjected to either 60 min-warm ischemia (WI) or auto-transplanted after cold storage for 24 h at 4°C (CS), or both conditions combined (WI + CS). (biomedcentral.com)
  • Laparoscopic partial nephrectomy in cold ischemia: renal artery perfusion. (semanticscholar.org)
  • Early Intraductal L-Glutamine Perfusion Improves Pig Islet Isolation Outcome After Warm and Cold Ischemia. (ox.ac.uk)
  • Thirty minutes after cardiac arrest, in situ anterograde selective pulmonary cold perfusion was started in six swine. (univoak.eu)
  • Based on this, there is a need for uniform perfusion settings (perfusion route and duration), and the development of general guidelines regarding the duration of cold storage in context of the overall donor risk is also required to better compare various trial results. (mdpi.com)
  • While the organ is being transported, it is either stored in an icy cold solution to help preserve it or it is connected to a miniature organ perfusion system which pumps an icy solution (sometimes enriched with potassium) through the organ. (wikipedia.org)
  • DI-fusion OKT3 prophylaxis in Renal Grafts with prolonged cold ischemia. (ac.be)
  • The objective of this study was to evaluate whether vaporized PFC is also protective of lung grafts at different cold ischemia times . (bvsalud.org)
  • Cold Ischemia And Renal Graft Survival: A Paired Analysis Comparing First And Second Grafts From The Same Donor. (com.es)
  • OBJECTIVE: To compare the characteristics, clinical course, and survival of pairs of renal grafts from the same donor, with special interest in cold ischemia times (CIT) as a risk factor for graft survival. (com.es)
  • To overcome such obstacles, we developed a novel reconditioning method for donor organs named hydrogen flush after cold storage (HyFACS), which is just an end-ischemic H 2 flush directly to donor organs ex vivo, and, herein, we report its therapeutic impact against hepatic IRI. (fujita-hu.ac.jp)
  • After 24-hour CS in UW solution, livers were cold-flushed with H 2 solution (1.0 ppm) via the portal vein (PV), the hepatic artery (HA), or both (PV + HA). (fujita-hu.ac.jp)
  • In addition to limb ischemia, other organs can become ischemic, causing: Renal ischemia (nephric ischemia) Mesenteric ischemia Cerebral ischemia Cardiac ischemia In order to treat acute limb ischaemia there are a series of things that can be done to determine where the occlusion is located, the severity, and what the cause was. (wikipedia.org)
  • Myocardial ischemia, also called cardiac ischemia, reduces the heart muscle's ability to pump blood. (mayoclinic.org)
  • Why does Exercise in Cold Air Increase Susceptibility to Myocardial Ischaemia? (vumc.nl)
  • Cold stress may also be used to model conditions where increases in myocardial oxygen demand are not met by anticipated increases in coronary blood flow, as occurs in older adults. (cdc.gov)
  • Myocardial ischemia occurs when blood flow to the heart muscle (myocardium) is obstructed by a partial or complete blockage of a coronary artery by a buildup of plaques (atherosclerosis). (mayoclinic.org)
  • Myocardial ischemia occurs when blood flow to your heart is reduced, preventing the heart muscle from receiving enough oxygen. (mayoclinic.org)
  • Myocardial ischemia might also cause serious abnormal heart rhythms. (mayoclinic.org)
  • Treatment for myocardial ischemia involves improving blood flow to the heart muscle. (mayoclinic.org)
  • Making heart-healthy lifestyle choices is important in treating and preventing myocardial ischemia. (mayoclinic.org)
  • Some people who have myocardial ischemia don't have any signs or symptoms (silent ischemia). (mayoclinic.org)
  • Myocardial ischemia occurs when the blood flow through one or more of your coronary arteries is decreased. (mayoclinic.org)
  • Atherosclerosis is the most common cause of myocardial ischemia. (mayoclinic.org)
  • The clot might block an artery and lead to sudden, severe myocardial ischemia, resulting in a heart attack. (mayoclinic.org)
  • Coronary artery spasm is an uncommon cause of myocardial ischemia. (mayoclinic.org)
  • Type 1 and type 2 diabetes are linked to an increased risk of myocardial ischemia, heart attack and other heart problems. (mayoclinic.org)
  • Jain S, Plenter R, Nydam T, Jani A. Cellular Sources of Cytokine Production during Cold Ischemia Induced AKI after Kidney Transplant [abstract]. (atcmeetingabstracts.com)
  • The use of these organs could be increased if "cold ischemia times" are limited. (medindia.net)
  • Given its safety, simplicity, and cost-effectiveness, end-ischemic HyFACS may be a novel pretransplant conditioning for cold-stored donor organs. (fujita-hu.ac.jp)
  • Scientists also did not repeat Dr. Suda's success for other organs those are more resistant to cold storage and freezing than the brain. (cryonics.org)
  • CONCLUSION: In this model, donor and recipient treatment with NAC effectively protected the lung from primary graft dysfunction after prolonged cold ischemia. (uzh.ch)
  • In this study, we investigated the paracrine effects of human amnion-derived MSCs (hAMSCs) in an in vitro model of lung IRI that includes cold ischemia and normothermic EVLP. (elsevier.com)
  • It therefore appears that hypothermia can prevent effectively mitochondrial dysfunction due to ischemia. (springer.com)
  • Ischemia Modified by Topical Hypothermia. (indigo.ca)
  • The process of restoring blood flow following this period of "cold ischemia" can lead to tissue damage. (medindia.net)
  • Signaling pathways regarding the pathophysiology of liver I/R and regeneration making clear distinction between situations of cold and warm ischemia, as well as liver regeneration with or without vascular occlusion, will be addressed. (intechopen.com)
  • Macrophages in both mouse and human lungs that have been subjected to cold ischemic storage express DAP12. (jimmunol.org)
  • We conclude that vaporized PFC reduces the production of free radicals and the number of pulmonary structural changes resulting from cold ischemia . (bvsalud.org)
  • Acute limb ischaemia (ALI) occurs when there is a sudden lack of blood flow to a limb. (wikipedia.org)
  • The same procedures with cooling using ice bags, as well as warm and cold sham-operations were performed. (bvsalud.org)
  • 3. The study of warm and cold ischemia of rat brain tissues. (cryonics.org)
  • it is the sum of warm and cold ischemia times. (thefreedictionary.com)
  • Cold ischemia and the reduced long-term survival of cadaveric renal allografts. (semanticscholar.org)
  • VRIS may be broadly applicable to provide an indicator of organ ischemia during open and laparoscopic procedures. (spiedigitallibrary.org)
  • PURPOSE The safe duration of ischemia during nephron sparing surgery remains controversial. (semanticscholar.org)
  • 12 There remains much controversy as to the specific duration of ischemia that results in renal injury and at what point cold ischemia should be employed. (spiedigitallibrary.org)
  • After l0 to 60 minutes of ischemia at 37°C the energy coupled respiration as well as the ADP-induced malate-aspartate shuttle activity in brain and liver mitochondria or the rate of mitochondrial ATP synthesis in kidney were significantly decreased. (springer.com)
  • It was 72% for 2 hours of warm ischemia and only 50% for 3 hours of warm exposure. (cryonics.org)
  • Brain slice viability was 80% of the control for 3 hours of cold (4oC) ischemia without warm ischemia. (cryonics.org)
  • It was 74% for 6 hours of cold ischemia, 59% for 12 hours, 50% for 18 hours, 43% for 24 hours, and 0% for 48 hours of cold exposure. (cryonics.org)
  • In some cases, gangrene can occur within six hours of ischaemia. (wikipedia.org)
  • These results indicate the risk of coagulopathy following limb I/R on early post-eventually days, which risk is higher in the case of cold I/R. (bvsalud.org)
  • Acute limb ischaemia is caused by embolism or thrombosis, or rarely by dissection or trauma. (wikipedia.org)
  • In this sense, ischaemia refers to the inhibition of blood flow to/through the limb. (wikipedia.org)
  • Acute limb ischaemia can occur in patients through all age groups. (wikipedia.org)
  • Patients that smoke and have diabetes mellitus are at a higher risk of developing acute limb ischaemia. (wikipedia.org)
  • When a limb is ischemic in the non-acute (chronic) setting, the condition is alternatively called peripheral artery disease or critical limb ischemia, rather than ALI. (wikipedia.org)
  • In the worst cases acute limb ischaemia progresses to critical limb ischaemia, and results in death or limb loss. (wikipedia.org)
  • Compartment syndrome can occur because of acute limb ischaemia because of the biotoxins that accumulate distal to the occlusion resulting in edema. (wikipedia.org)
  • The primary intervention in acute limb ischaemia is emergency embolectomy using a Fogarty Catheter, providing the limb is still viable within the 4-6h timeframe. (wikipedia.org)
  • Greater length of renal ischemia is associated with subsequent worse recovery of renal function, whereas cooling of the kidney allows for longer exposure to ischemia. (spiedigitallibrary.org)
  • Exposure to acute heat or cold stress elicits numerous physiological responses aimed at maintaining body temperatures. (cdc.gov)