Cold Ischemia: The chilling of a tissue or organ during decreased BLOOD perfusion or in the absence of blood supply. Cold ischemia time during ORGAN TRANSPLANTATION begins when the organ is cooled with a cold perfusion solution after ORGAN PROCUREMENT surgery, and ends after the tissue reaches physiological temperature during implantation procedures.Ischemia: A hypoperfusion of the BLOOD through an organ or tissue caused by a PATHOLOGIC CONSTRICTION or obstruction of its BLOOD VESSELS, or an absence of BLOOD CIRCULATION.Organ Preservation: The process by which organs are kept viable outside of the organism from which they were removed (i.e., kept from decay by means of a chemical agent, cooling, or a fluid substitute that mimics the natural state within the organism).Cold Temperature: An absence of warmth or heat or a temperature notably below an accustomed norm.Organ Preservation Solutions: Solutions used to store organs and minimize tissue damage, particularly while awaiting implantation.Reperfusion Injury: Adverse functional, metabolic, or structural changes in ischemic tissues resulting from the restoration of blood flow to the tissue (REPERFUSION), including swelling; HEMORRHAGE; NECROSIS; and damage from FREE RADICALS. The most common instance is MYOCARDIAL REPERFUSION INJURY.Raffinose: A trisaccharide occurring in Australian manna (from Eucalyptus spp, Myrtaceae) and in cottonseed meal.Myocardial Ischemia: A disorder of cardiac function caused by insufficient blood flow to the muscle tissue of the heart. The decreased blood flow may be due to narrowing of the coronary arteries (CORONARY ARTERY DISEASE), to obstruction by a thrombus (CORONARY THROMBOSIS), or less commonly, to diffuse narrowing of arterioles and other small vessels within the heart. Severe interruption of the blood supply to the myocardial tissue may result in necrosis of cardiac muscle (MYOCARDIAL INFARCTION).Brain Ischemia: Localized reduction of blood flow to brain tissue due to arterial obstruction or systemic hypoperfusion. This frequently occurs in conjunction with brain hypoxia (HYPOXIA, BRAIN). Prolonged ischemia is associated with BRAIN INFARCTION.Delayed Graft Function: General dysfunction of an organ occurring immediately following its transplantation. The term most frequently refers to renal dysfunction following KIDNEY TRANSPLANTATION.Graft Survival: The survival of a graft in a host, the factors responsible for the survival and the changes occurring within the graft during growth in the host.Tissue Donors: Individuals supplying living tissue, organs, cells, blood or blood components for transfer or transplantation to histocompatible recipients.Warm Ischemia: A tissue or organ remaining at physiological temperature during decreased BLOOD perfusion or in the absence of blood supply. During ORGAN TRANSPLANTATION it begins when the organ reaches physiological temperature before the completion of SURGICAL ANASTOMOSIS and ends with reestablishment of the BLOOD CIRCULATION through the tissue.Kidney Transplantation: The transference of a kidney from one human or animal to another.Liver Transplantation: The transference of a part of or an entire liver from one human or animal to another.Allopurinol: A XANTHINE OXIDASE inhibitor that decreases URIC ACID production. It also acts as an antimetabolite on some simpler organisms.Tissue and Organ Procurement: The administrative procedures involved with acquiring TISSUES or organs for TRANSPLANTATION through various programs, systems, or organizations. These procedures include obtaining consent from TISSUE DONORS and arranging for transportation of donated tissues and organs, after TISSUE HARVESTING, to HOSPITALS for processing and transplantation.Cadaver: A dead body, usually a human body.Time Factors: Elements of limited time intervals, contributing to particular results or situations.Common Cold: A catarrhal disorder of the upper respiratory tract, which may be viral or a mixed infection. It generally involves a runny nose, nasal congestion, and sneezing.Transplantation, Isogeneic: Transplantation between genetically identical individuals, i.e., members of the same species with identical histocompatibility antigens, such as monozygotic twins, members of the same inbred strain, or members of a hybrid population produced by crossing certain inbred strains.Cryopreservation: Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.Procaine: A local anesthetic of the ester type that has a slow onset and a short duration of action. It is mainly used for infiltration anesthesia, peripheral nerve block, and spinal block. (From Martindale, The Extra Pharmacopoeia, 30th ed, p1016).Tissue and Organ Harvesting: The procedure of removing TISSUES, organs, or specimens from DONORS for reuse, such as TRANSPLANTATION.Rats, Inbred LewTransplants: Organs, tissues, or cells taken from the body for grafting into another area of the same body or into another individual.Trimetazidine: A vasodilator used in angina of effort or ischemic heart disease.Brain Death: A state of prolonged irreversible cessation of all brain activity, including lower brain stem function with the complete absence of voluntary movements, responses to stimuli, brain stem reflexes, and spontaneous respirations. Reversible conditions which mimic this clinical state (e.g., sedative overdose, hypothermia, etc.) are excluded prior to making the determination of brain death. (From Adams et al., Principles of Neurology, 6th ed, pp348-9)Death: Irreversible cessation of all bodily functions, manifested by absence of spontaneous breathing and total loss of cardiovascular and cerebral functions.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.Myocardial Reperfusion Injury: Damage to the MYOCARDIUM resulting from MYOCARDIAL REPERFUSION (restoration of blood flow to ischemic areas of the HEART.) Reperfusion takes place when there is spontaneous thrombolysis, THROMBOLYTIC THERAPY, collateral flow from other coronary vascular beds, or reversal of vasospasm.Mannitol: A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.Pancreas Transplantation: The transference of a pancreas from one human or animal to another.Graft Rejection: An immune response with both cellular and humoral components, directed against an allogeneic transplant, whose tissue antigens are not compatible with those of the recipient.Adenosine: A nucleoside that is composed of ADENINE and D-RIBOSE. Adenosine or adenosine derivatives play many important biological roles in addition to being components of DNA and RNA. Adenosine itself is a neurotransmitter.Rats, Wistar: A strain of albino rat developed at the Wistar Institute that has spread widely at other institutions. This has markedly diluted the original strain.Hypothermia, Induced: Abnormally low BODY TEMPERATURE that is intentionally induced in warm-blooded animals by artificial means. In humans, mild or moderate hypothermia has been used to reduce tissue damages, particularly after cardiac or spinal cord injuries and during subsequent surgeries.Rats, Sprague-Dawley: A strain of albino rat used widely for experimental purposes because of its calmness and ease of handling. It was developed by the Sprague-Dawley Animal Company.Transplantation, Homologous: Transplantation between individuals of the same species. Usually refers to genetically disparate individuals in contradistinction to isogeneic transplantation for genetically identical individuals.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.Ischemic Attack, Transient: Brief reversible episodes of focal, nonconvulsive ischemic dysfunction of the brain having a duration of less than 24 hours, and usually less than one hour, caused by transient thrombotic or embolic blood vessel occlusion or stenosis. Events may be classified by arterial distribution, temporal pattern, or etiology (e.g., embolic vs. thrombotic). (From Adams et al., Principles of Neurology, 6th ed, pp814-6)Reperfusion: Restoration of blood supply to tissue which is ischemic due to decrease in normal blood supply. The decrease may result from any source including atherosclerotic obstruction, narrowing of the artery, or surgical clamping. It is primarily a procedure for treating infarction or other ischemia, by enabling viable ischemic tissue to recover, thus limiting further necrosis. However, it is thought that reperfusion can itself further damage the ischemic tissue, causing REPERFUSION INJURY.Disease Models, Animal: Naturally occurring or experimentally induced animal diseases with pathological processes sufficiently similar to those of human diseases. They are used as study models for human diseases.Living Donors: Non-cadaveric providers of organs for transplant to related or non-related recipients.Perfusion: Treatment process involving the injection of fluid into an organ or tissue.Retrospective Studies: Studies used to test etiologic hypotheses in which inferences about an exposure to putative causal factors are derived from data relating to characteristics of persons under study or to events or experiences in their past. The essential feature is that some of the persons under study have the disease or outcome of interest and their characteristics are compared with those of unaffected persons.Liver Failure: Severe inability of the LIVER to perform its normal metabolic functions, as evidenced by severe JAUNDICE and abnormal serum levels of AMMONIA; BILIRUBIN; ALKALINE PHOSPHATASE; ASPARTATE AMINOTRANSFERASE; LACTATE DEHYDROGENASES; and albumin/globulin ratio. (Blakiston's Gould Medical Dictionary, 4th ed)Tissue Fixation: The technique of using FIXATIVES in the preparation of cytologic, histologic, or pathologic specimens for the purpose of maintaining the existing form and structure of all the constituent elements.Ischemic Preconditioning: A technique in which tissue is rendered resistant to the deleterious effects of prolonged ISCHEMIA and REPERFUSION by prior exposure to brief, repeated periods of vascular occlusion. (Am J Physiol 1995 May;268(5 Pt 2):H2063-7, Abstract)Glutathione: A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides.Cold Climate: A climate characterized by COLD TEMPERATURE for a majority of the time during the year.Models, Animal: Non-human animals, selected because of specific characteristics, for use in experimental research, teaching, or testing.Immunohistochemistry: Histochemical localization of immunoreactive substances using labeled antibodies as reagents.Heart Transplantation: The transference of a heart from one human or animal to another.Gerbillinae: A subfamily of the Muridae consisting of several genera including Gerbillus, Rhombomys, Tatera, Meriones, and Psammomys.Spinal Cord Ischemia: Reduced blood flow to the spinal cord which is supplied by the anterior spinal artery and the paired posterior spinal arteries. This condition may be associated with ARTERIOSCLEROSIS, trauma, emboli, diseases of the aorta, and other disorders. Prolonged ischemia may lead to INFARCTION of spinal cord tissue.Nephrectomy: Excision of kidney.Postoperative Complications: Pathologic processes that affect patients after a surgical procedure. They may or may not be related to the disease for which the surgery was done, and they may or may not be direct results of the surgery.Myocardium: The muscle tissue of the HEART. It is composed of striated, involuntary muscle cells (MYOCYTES, CARDIAC) connected to form the contractile pump to generate blood flow.Lung Transplantation: The transference of either one or both of the lungs from one human or animal to another.Ischemic Preconditioning, Myocardial: Exposure of myocardial tissue to brief, repeated periods of vascular occlusion in order to render the myocardium resistant to the deleterious effects of ISCHEMIA or REPERFUSION. The period of pre-exposure and the number of times the tissue is exposed to ischemia and reperfusion vary, the average being 3 to 5 minutes.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Treatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.P-Selectin: Cell adhesion molecule and CD antigen that mediates the adhesion of neutrophils and monocytes to activated platelets and endothelial cells.Liver Diseases: Pathological processes of the LIVER.Transplantation Immunology: A general term for the complex phenomena involved in allo- and xenograft rejection by a host and graft vs host reaction. Although the reactions involved in transplantation immunology are primarily thymus-dependent phenomena of cellular immunity, humoral factors also play a part in late rejection.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Neuroprotective Agents: Drugs intended to prevent damage to the brain or spinal cord from ischemia, stroke, convulsions, or trauma. Some must be administered before the event, but others may be effective for some time after. They act by a variety of mechanisms, but often directly or indirectly minimize the damage produced by endogenous excitatory amino acids.Potassium Chloride: A white crystal or crystalline powder used in BUFFERS; FERTILIZERS; and EXPLOSIVES. It can be used to replenish ELECTROLYTES and restore WATER-ELECTROLYTE BALANCE in treating HYPOKALEMIA.Infarction, Middle Cerebral Artery: NECROSIS occurring in the MIDDLE CEREBRAL ARTERY distribution system which brings blood to the entire lateral aspects of each CEREBRAL HEMISPHERE. Clinical signs include impaired cognition; APHASIA; AGRAPHIA; weak and numbness in the face and arms, contralaterally or bilaterally depending on the infarction.Myocardial Reperfusion: Generally, restoration of blood supply to heart tissue which is ischemic due to decrease in normal blood supply. The decrease may result from any source including atherosclerotic obstruction, narrowing of the artery, or surgical clamping. Reperfusion can be induced to treat ischemia. Methods include chemical dissolution of an occluding thrombus, administration of vasodilator drugs, angioplasty, catheterization, and artery bypass graft surgery. However, it is thought that reperfusion can itself further damage the ischemic tissue, causing MYOCARDIAL REPERFUSION INJURY.Specimen Handling: Procedures for collecting, preserving, and transporting of specimens sufficiently stable to provide accurate and precise results suitable for clinical interpretation.Intercellular Adhesion Molecule-1: A cell-surface ligand involved in leukocyte adhesion and inflammation. Its production is induced by gamma-interferon and it is required for neutrophil migration into inflamed tissue.Risk Factors: An aspect of personal behavior or lifestyle, environmental exposure, or inborn or inherited characteristic, which, on the basis of epidemiologic evidence, is known to be associated with a health-related condition considered important to prevent.Adenosine Triphosphate: An adenine nucleotide containing three phosphate groups esterified to the sugar moiety. In addition to its crucial roles in metabolism adenosine triphosphate is a neurotransmitter.Histocompatibility Testing: Identification of the major histocompatibility antigens of transplant DONORS and potential recipients, usually by serological tests. Donor and recipient pairs should be of identical ABO blood group, and in addition should be matched as closely as possible for HISTOCOMPATIBILITY ANTIGENS in order to minimize the likelihood of allograft rejection. (King, Dictionary of Genetics, 4th ed)Mice, Inbred C57BLCerebrovascular Circulation: The circulation of blood through the BLOOD VESSELS of the BRAIN.Patient Selection: Criteria and standards used for the determination of the appropriateness of the inclusion of patients with specific conditions in proposed treatment plans and the criteria used for the inclusion of subjects in various clinical trials and other research protocols.Coronary Circulation: The circulation of blood through the CORONARY VESSELS of the HEART.Reoperation: A repeat operation for the same condition in the same patient due to disease progression or recurrence, or as followup to failed previous surgery.Survival Rate: The proportion of survivors in a group, e.g., of patients, studied and followed over a period, or the proportion of persons in a specified group alive at the beginning of a time interval who survive to the end of the interval. It is often studied using life table methods.Hindlimb: Either of two extremities of four-footed non-primate land animals. It usually consists of a FEMUR; TIBIA; and FIBULA; tarsals; METATARSALS; and TOES. (From Storer et al., General Zoology, 6th ed, p73)Brain: The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.

Calcium channel blocker and renal mitochondrial function in warm renal ischemia. (1/125)

OBJECTIVE: Ions, particularly calcium ions, play an important role in ischemia-reperfusion cell injury. In this study, we investigated the action of verapamil on the mitochondrial function of kidneys submitted to ischemia without blood reperfusion in order to study isolated early and late ischemic effects. MATERIALS AND METHODS: 44 rats were submitted to bilateral warm renal ischemia for 30 minutes. The kidneys were then immediately reperfused with saline or Euro-Collins (EC) solution, with and without previous administration of 0.35 mg/kg of verapamil. Mitochondrial function was assessed at the end of renal perfusion and after 24 hours of cold preservation. RESULTS: In kidneys perfused with saline, verapamil allowed a significant early preservation of state III mitochondrial respiration, a result that was no longer evident after 24 hours. In kidneys perfused with EC solution, verapamil did not change state III for either early or late evaluations. Comparison of the groups showed that the results obtained for kidneys perfused with EC were always superior to those obtained for the saline group, except for the initial analysis of kidneys treated with saline and verapamil, which showed results similar to those obtained with EC perfusion alone. CONCLUSION: Administration of verapamil before warm ischemia provides partial and short-lasting functional protection of the mitochondrial function in kidneys perfused with sodium rich saline. With Euro-Collins solution, verapamil did not show any additional beneficial effect. This fact permits us to conclude that protective action is effective only under conditions that facilitate increased sodium uptake and/or potassium loss.  (+info)

Major effects of delayed graft function and cold ischaemia time on renal allograft survival. (2/125)

BACKGROUND: There is mounting evidence from experimental and clinical studies that the quality of organs from cadaver donors may be influenced by events occurring around the time of brain death, and that these may affect transplant outcome. The aim of this study is to investigate the influence of donor factors on renal allograft outcome in a homogeneous cohort of 518 patients transplanted in a single centre over a 9 year period. METHODS: Endpoints of the study were delayed graft function (DGF), acute rejection (AR), 1 year graft survival and long-term survival of those grafts that reached 1 year. Multivariate analysis was performed to determine factors that may have influenced the graft outcome indicators. RESULTS: DGF was the major predictor of graft failure overall with cold ischaemia time (CIT) as an important independent factor. The level of histocompatibility did not influence graft survival. DGF was the major factor affecting 1 year graft survival (P<0.0005) with effects persisting beyond 1 year. DGF was significantly influenced by CIT, donor age, female kidney into male recipient and donor creatinine (P<0.05). Other donor factors and factors associated with donor management were not risk factors for DGF, rejection episodes or graft survival. The risk factors for a number of AR episodes were HLA-DR mismatch and DGF (P<0.005). When grafts surviving for 1 year were considered, only CIT, recipient age and creatinine at 1 year (P<0.05) were found to affect graft survival significantly. CONCLUSIONS: The results of this analysis of well-matched transplant recipients show that CIT and DGF are the most important predictors of poor short and long-term graft survival. Therefore, in order to improve the long-term survival of renal allografts efforts should focus on limiting CIT and the damage that occurs during this period and on improving our understanding of DGF.  (+info)

Glycine intravenous donor preconditioning is superior to glycine supplementation to low-potassium dextran flush preservation and improves graft function in a large animal lung transplantation model after 24 hours of cold ischemia. (3/125)

OBJECTIVES: The potential role of glycine in combination with standard lung preservation with low-potassium dextran solution in lung ischemia-reperfusion injury has not been investigated in a preclinical porcine transplant model. METHODS: In a control group (n = 6), donor lungs were flushed with 1 liter of low-potassium dextran solution. In a second group (LPD-glyc, n = 6), low-potassium dextran solution was supplemented with 3.75 g of glycine. In a third group (IV-glyc, n = 6), donor preconditioning was performed by intravenous administration of 3.75 g glycine 1 hour before low-potassium dextran preservation. Grafts were stored in low-potassium dextran at 4 degrees C for 24 hours. Posttransplant graft function was assessed throughout a 7-hour observation period. RESULTS: In the control group, 2 recipients died of right-sided heart failure caused by severe ischemia-reperfusion injury. All animals of the glycine groups survived the entire observation period. Pulmonary vascular resistance remained significantly (P < .01) lower in both glycine groups when compared with controls. At the end of the observation period pulmonary vascular resistance in the control group was higher (P < .01) compared with the glycine groups (1310 +/- 319 dyn x sec x cm(-5) vs 879 +/- 127 dyn x sec x cm(-5) [LPD-glyc] vs 663 +/- 191 dyn x sec x cm(-5) [IV-glyc]). Changes of lung tissue water content were lower in the IV-glyc group compared with the LPD-control (P < .01) and LPD-glyc lungs (P < .05). Oxygenation (PO2/FiO2) was higher in the IV-glyc group compared with the LPD-glyc and control lungs (445 +/- 110 mm Hg vs 388 +/- 124 mm Hg [P < .01] vs 341 +/- 224 mm Hg [P < .001], respectively). DISCUSSION: Modification of low-potassium dextran solution with glycine or donor preconditioning ameliorates ischemia-reperfusion injury in lung transplantation. This intriguing approach merits further evaluation with respect to the mechanisms involved and may improve results in clinical lung preservation.  (+info)

In situ demonstration of improvement of liver mitochondria function by melatonin after cold ischemia. (4/125)

In a previous investigation, reperfusion with a melatonin-containing medium was demonstrated to enhance bile production and tissue ATP levels in rat livers, cold-preserved with University of Wisconsin (UW) or Celsior solutions, with respect to melatonin-free reperfusion; lipid peroxidation products in the perfusate were not influenced by the indole. This was ascribed to an increased efficiency of the hepatocyte mitochondria induced by melatonin. Reactive oxygen species (ROS) normally leak from the electron transfer chain in mitochondria and excessive ROS production is presumed to mediate ischemia-reperfusion (I/R) damage. A histochemical reaction was used to demonstrate ROS on the same model. Compared to the lobular zonation of ROS in control livers, the stained area of cold-preserved livers reperfused without melatonin was restricted to a narrow portal region, in keeping with the much lower ATP content. When reperfusion was performed with melatonin, the liver morphology was improved and the ROS reaction in hepatocytes more intense, though not reaching the control liver pattern. Sinusoidal cells were poorly-stained in both cases. In conclusion, with this different approach, melatonin was confirmed to improve mitochondrial performance and to discriminate parenchymal from sinusoidal cell behavior. Our observations confirm that melatonin mitigates I/R injury and support its potential in liver transplantation.  (+info)

Cardioprotective effects of tetrahydrobiopterin in cold heart preservation after cardiac arrest. (5/125)

BACKGROUND: It has recently been shown that tetrahydrobiopterin (BH4), an essential cofactor of nitric oxide synthase (NOS), reduces ischemia-reperfusion myocardial injury. The aim of this study was to determine if supplementation with BH4 after cardiac arrest followed by cold heart preservation would exert a cardioprotective effect against ischemia-reperfusion injury. MATERIALS AND METHODS: Isolated perfused rat hearts were subjected to 4 degrees C cold ischemia and reperfusion. Hearts were treated with cold cardioplegic solution with or without BH4 just before ischemia and during the first 5 min of reperfusion period. Effects of BH4 on left ventricular function, myocardial contents of high-energy phosphates, and nitrite plus nitrate were measured in the perfusate, before ischemia and after reperfusion. Moreover, the effect of BH4 on the cold-heart preservation followed by normothermic (37 degrees C) ischemia was determined. RESULTS: BH4 improved the contractile and metabolic abnormalities in reperfused cold preserved hearts that were subjected to normothermic ischemia. Furthermore, BH4 significantly alleviated ischemic contracture during ischemia, and restored the diminished perfusate levels of nitrite plus nitrate after reperfusion. CONCLUSION: These results demonstrated that BH4 reduces ischemia-reperfusion injury in cold heart preservation. The cardioprotective effect of BH4 implies that BH4 could be a novel and effective therapeutic option in the preservation treatment of donor heart after cardiac arrest.  (+info)

Mediators of rat ischemic hepatic preconditioning after cold preservation identified by microarray analysis. (6/125)

Hepatic ischemia-reperfusion injury associated with liver transplantation is an as yet unresolved problem in clinical practice. Preconditioning protects the liver against the deleterious effects of ischemia, although the mechanism underlying this preconditioning is still unclear. To profile gene expression patterns involved in hepatic ischemic preconditioning, we analyzed the changes in gene expression in rat livers by DNA microarray analysis. Approximately 116 genes were found to have altered gene expression after 8 hours of cold ischemia. Moreover, the expression of 218 genes was modified by classic preconditioning followed by the same ischemia process. Given the importance of the effects of ischemic preconditioning (IP) in minimizing the liver damage induced by sustained ischemia before reperfusion, this study analyzed the putative genes involved in the beneficial role of IP in liver grafts undergoing cold ischemia before its implantation in the recipient (IP+I). Great differences were found in the gene expression pattern of ischemic preconditioning + long cold ischemia (IP+I) group when compared with the long cold ischemia alone condition (I), which could explain the protective regulatory mechanisms that take place after preconditioning. Twenty-six genes that were downregulated in cold ischemia were found upregulated after preconditioning preceding a long cold ischemia period. These would be genes activated or maintained by preconditioning. Heat shock protein genes and 3-hydroxy-3-methylglutaryl-coenzyme A reductase are among the most markedly induced transcripts.  (+info)

Prolonging warm ischemia reduces the cold preservation limits of liver grafts in swine. (7/125)

BACKGROUND: The critical shortage of transplantable organs necessitates utilization of unconventional donors. But the safe time limits of cold preservation of liver grafts subjected to warm ischemia (WI) for up to 30 minutes from non-heart-beating-donors (NHBDs) has not been delineated. In this study, we investigated how the limits of cold ischemia (CI) in University of Wisconsin (UW) solution are changed in liver grafts subjected to WI from 10 to 30 minutes. METHODS: A simple porcine NHBD liver transplantation (LT) model was developed. In donors, livers were subjected to 10, 20 or 30 minutes of WI and subsequent different times of CI in UW solution. Animals were divided into three groups (WI 10 min, WI 20 min, WI 30 min, n=13 in each group) and nine subgroups (from CI 6 h to CI 28 h). One-week survival rates of recipients, hepatic function, liver energy metabolism, grafted liver microcirculation and pathological observations of the liver were compared. RESULTS: In the WI 10 min group, the one-week survival rate of the CI 20 h subgroup was significantly higher than in the other two subgroups (CI 24 h and CI 28 h) (P<0.05). Furthermore, the CI 20 h subgroup had a lower level of alanine aminotransferase (ALT), less pathological damage, a higher concentration of adenosine triphosphate (ATP) and microcirculatory blood flow in the grafted livers at 1 hour after reperfusion than the other two subgroups. The same trends were also found in the other two groups (WI 20 min and WI 30 min) and their subgroups. CONCLUSIONS: The cold preservation limits of the liver grafts shortened from 20 to 12 to 6 hours when WI time was prolonged from 10 to 20 to 30 minutes. Only the liver grafts within these time limits could be safely transplanted.  (+info)

Novel short-term hypothermic oxygenated perfusion (HOPE) system prevents injury in rat liver graft from non-heart beating donor. (8/125)

OBJECTIVE: To assess a machine perfusion system in rescuing liver grafts from non-heart-beating donors (NHBD). SUMMARY BACKGROUND DATA: The introduction of extracorporeal liver perfusion systems in the clinical routine depends on feasibility. Conceivably, perfusion could be performed during recipient preparation. We investigated whether a novel rat liver machine perfusion applied after in situ ischemia and cold storage can rescue NHBD liver grafts. METHODS: We induced cardiac arrest in male Brown Norway rats by phrenotomy and ligation of the subcardial aorta. We studied 2 experimental groups: 45 minutes of warm in situ ischemia + 5 hours cold storage versus 45 minutes of warm in situ ischemia + 5 hours cold storage followed by 1 hour hypothermic oxygenated extracorporeal perfusion (HOPE). In both groups, livers were reperfused in a closed sanguineous isolated liver perfusion device for 3 hours at 37 degrees C. To test the benefit of HOPE on survival, we performed orthotopic liver transplantation in both experimental groups. RESULTS: After cold storage and reperfusion, NHBD livers showed necrosis of hepatocytes, increased release of AST, and decreased bile flow. HOPE improved NHBD livers significantly with a reduction of necrosis, less AST release, and increased bile flow. ATP was severely depleted in cold-stored NHBD livers but restored in livers treated by HOPE. After orthotopic liver transplantation, grafts treated by HOPE demonstrated a significant extension on animal survival. CONCLUSIONS: We demonstrate a beneficial effect of HOPE by preventing reperfusion injury in a clinically relevant NHBD model.  (+info)

*Organ procurement

For liver transplants, the cold ischemia time can be up to 24 hours, although typically surgeons aim for a much shorter period ... This time during transport is called the "cold ischemia time". Heart and lungs should have less than 6 hours between organ ... For kidney transplants, as the cold ischemia time increases, the risk of delayed function of the kidney increases. Sometimes, ... While the organ is being transported, it is stored in an icy cold solution to help preserve it. ...

*Uterus transplantation

Studies on cold-ischemia/eperfusion indicate an ischemic tolerance of more than 24 hours. The recipient has to look at ...

*Mehmet Haberal

1996) Ten-year survival after a cold-ischemia time of 111 hours in the transplanted kidney. Transplantation Proceedings. 28(4): ... 1979 He performed successful deceased-donor kidney transplants with a cold ischemia time of 111 hours, a first in the medical ...

*Kidney Paired Donation

"Each additional hour of cold ischemia time significantly increases the risk of graft failure and mortality following renal ... Concerns regarding the negative impact that Cold Ischemic Time (CIT) may have on graft survival, due to the shipment of the ...

*Intestine transplantation

Furthermore, irreversible intestinal damage is seen after approximately only 5 hours of cold ischemia in the form of mucosal ... mesenteric ischemia, motility disorder, omphalocele/gastroschisis, tumors, and volvulus. Regardless of the underlying condition ... and serum pH and lactate levels measured for evidence of intestinal ischemia. The patient's immune system is strongly modulated ... extensive venous thrombosis or arterial ischemia of the mesentery, and motility syndromes. Donated intestines, like all organs ...

*Immortalist Society

... improve perfusion protocol and find formulations to minimize cold ischemia (a concern for organ transplantation). Dr. Pichugin ...

*Penile injury

... though fewer than 16 hours of cold ischemia or 6 hours of warm ischemia leads to the best outcomes. If replantation is not ...

*Surgical staple

... at the back table in cold ischemia condition for the donor organ and after native organ removal in the recipient. This is ... finalized to make as brief as possible the donor organ dangerous warm ischemia phase that can be contained in the couple of ...

*List of MeSH codes (E04)

... cold ischemia MeSH E04.936.450 --- organ transplantation MeSH E04.936.450.050 --- bone transplantation MeSH E04.936.450.050.100 ... warm ischemia MeSH E04.950.165 --- castration MeSH E04.950.165.679 --- orchiectomy MeSH E04.950.165.685 --- ovariectomy MeSH ...

*Cysteinyl leukotriene receptor 1

... ischemia-, and cold-induced), multiple sclerosis, auto-immune encephalomyelitis, Alzheimer's disease, and Parkinson's disease. ... and cold-air induced asthma (see Exercise-induced bronchoconstriction); and childhood sleep apnea due to adenotonsillar ...

*Acute limb ischaemia

Symptoms of acute limb ischaemia include: Pain Pallor Paresthesias Perishingly cold Pulselessness Paralysis These symptoms are ... Renal ischemia (nephric ischemia) Mesenteric ischemia Cerebral ischemia Cardiac ischemia In order to treat acute limb ischaemia ... Acute limb ischaemia (ALI) occurs when there is a sudden lack of blood flow to a limb. Acute limb ischaemia is caused by ... The New Latin term ischaemia as written, is a British version of the word ischemia, and stems from the Greek terms ischein 'to ...

*Scintigraphy

Areas of infarct or ischemic tissue will remain "cold". Pre- and post-stress thallium may indicate areas that will benefit from ... This produces coronary steal from areas of ischemia where arteries are already maximally dilated. ...

*Drowning

Most victims of cold-water drowning do not develop hypothermia quickly enough to decrease cerebral metabolism before ischemia ... The actual cause of death in cold or very cold water is usually lethal bodily reactions to increased heat loss and to freezing ... This is because cold water can have other lethal effects on the body, so hypothermia is not usually a reason for drowning or ... Because of the diving reflex, people submerged in cold water and apparently drowned may revive after a relatively long period ...

*Ischemia

... as in acrobatics and military flying Localized extreme cold, such as by frostbite or improper cold compression therapy ... Ischemia of the small bowel is called mesenteric ischemia. Brain ischemia is insufficient blood flow to the brain, and can be ... Chronic ischemia of the brain may result in a form of dementia called vascular dementia. A brief episode of ischemia affecting ... Ischemia or ischaemia is a restriction in blood supply to tissues, causing a shortage of oxygen and needed for cellular ...

*Human serum albumin

"Ischemia-modified albumin reduction after coronary bypass surgery is associated with the cardioprotective efficacy of cold- ...

*Organ donation

Location of a transplant center with respect to a donor hospital is given priority due to the effects of Cold Ischemic Time ( ... due to ischemia. Perfusion devices, often called kidney pumps, can extend graft survival to 36-48 hours post recovery for ... Developments in kidney preservation have yielded a device that pumps cold preservation solution through the kidneys vessels to ... ischemia). Each organ tolerates different ischemic times. Hearts and lungs need to be transplanted within 4-6 hours from ...

*Erythema induratum

When weather is cold, ankle is cold, blue and often tender. In hot weather, ankle becomes hot, edematous, swollen and painful. ... Abnormal arterial supply causes low-grade ischemia of ankle region. The ankle skin becomes sensitive to temperature changes. ...

*Livedoid dermatitis

Skin biopsies of the site show necrosis caused by ischemia. Radiographic imaging may help to delineate the extent of the wound ... or cold compresses applied to the wound. The diagnosis is mainly clinical. ... Microscopic examination of affected tissue shows ischemic necrosis, and so various hypotheses exist to explain this ischemia, ...

*Chronic wound

... among which are ischemia, reperfusion injury, and bacterial colonization. Ischemia is an important factor in the formation and ... Blood vessels constrict in tissue that becomes cold and dilate in warm tissue, altering blood flow to the area. Thus keeping ... Like ischemia, bacterial colonization and infection damage tissue by causing a greater number of neutrophils to enter the wound ... Ischemia results from the dysfunction and, combined with reperfusion injury, causes the tissue damage that leads to the wounds ...

*Labyrinthitis

Acute localized ischemia of these structures also may be an important cause, especially in children, vestibular neuritis may be ... Some people will report having an upper respiratory infection (common cold) or flu prior to the onset of the symptoms of ... 30% of affected people had a common cold prior to developing the disease. Either bacterial or viral labyrinthitis can cause ... preceded by symptoms of a common cold. However, the causative mechanism remains uncertain. This can also be brought on by ...

*Alveolar osteitis

Therefore, cold fluids and foods are encouraged, which facilitate clot formation and prevent its disintegration. Avoiding ... It reduces the blood supply, leading to tissue ischemia, reduced tissue perfusion and eventually higher incidence of painful ...

*Cardioplegia

The cold fluid (usually at 4 °C) ensures that the heart cools down to a temperature of around 15-20 °C, thus slowing down the ... This process protects the myocardium, or heart muscle, from damage during the period of ischemia. To achieve this, the patient ... 1993 "Cold Crystalloid Cardioplegia" Hans J. Geissler* and Uwe Mehlhorn, Department of Cardiothoracic Surgery, University of ... It is employed as another means to further lower myocardial metabolism during periods of ischemia. The Van 't Hoff equation ...

*Toothache

A healthy tooth will feel the cold but the pain will be mild and disappear once the stimulus is removed. The accuracy of these ... This may lead to ischemia (lack of oxygen) and necrosis (tissue death). Pulpitis is termed reversible when the inflamed pulp is ... Hot drinks can make the tooth feel worse because they expand the gases and likewise, cold can make it feel better, thus some ... Pulp sensibility test using ethyl chloride (cold stimulus) Electric pulp tester Plastic wedge to identify pain on biting from a ...

*Blue toe syndrome

... but without diffuse forefoot ischemia. The development of blue or violaceous toes can also occur with trauma, cold-induced ... Blue toe syndrome is a situation that may reflect atherothrombotic microembolism, causing transient focal ischaemia, ...

*2-Bromo-LSD

Harvey JA (2003). "Role of the serotonin 5-HT(2A) receptor in learning". Learning & Memory (Cold Spring Harbor, N.Y.). 10 (5): ... Zivin JA, Venditto JA (April 1984). "Experimental CNS ischemia: serotonin antagonists reduce or prevent damage". Neurology. 34 ...

*Sumatriptan

Events reported have included coronary artery vasospasm, transient myocardial ischemia, myocardial infarction, ventricular ... paresthesias and warm/cold sensations) reported by 4% in the placebo group and 5-6% in the sumatriptan groups, pain and other ...
TY - JOUR. T1 - Ex Vivo Transfer of Nuclear Factor-κB Decoy Ameliorates Hepatic Cold Ischemia/Reperfusion Injury. AU - Yoshizumi, Tomoharu. AU - Ikeda, Yasuhiro. AU - Kaneda, Y.. AU - Sueishi, K.. PY - 2009/6/1. Y1 - 2009/6/1. N2 - Cold ischemia/reperfusion injury of the hepatic graft has been attributed to the release of various inflammatory cytokines. Specific inhibition of these cytokines may improve viability of the hepatic graft upon reperfusion. Herein we have assessed the efficacy of cis element decoy against nuclear factor-κB binding site delivery to the hepatic tissue in a rodent liver transplantation model. At 8 hours after reperfusion of the liver, significant reduction was noted in the livers treated with decoy in the release of cytosolic enzymes from the hepatocytes and in serum tumor necrosis factor α (P , .05). The neutrophilic infiltration into the hepatic grafts was significantly suppressed in the livers treated with decoy oligodeoxynucleotides (ODNs). Decoy ODNs against ...
Looking for cold ischemia time? Find out information about cold ischemia time. sequential arrangement of all events, or the interval between two events in such a sequence. The concept of time may be discussed on several different... Explanation of cold ischemia time
The effects of cold storage using Custodiol® (Histidine-Tryptophan-Ketoglutarate, HTK) or isotonic saline solution on mitochondrial function in hearts (left and rights ventricles) and various blood vessels of pigs were investigated. Hearts, saphenous veins, internal-mammary-arteries and aortas of male landrace pigs were harvested and exposed to cold ischemia in either saline or Custodiol-HTK solution. Mitochondrial function was measured in situ in permeabilized fibers by high-resolution respirometry. Mitochondrial respiratory capacities (maximal respiration rates) were similar in the right and left ventricle in controls and after 14 h of cold storage were significantly better preserved in Custodiol-HTK than in saline solution. Mitochondrial respiration rates in various blood vessels including aorta, arteries and veins were less than 5% of myocardium rates. In contrast to the pig heart, in some blood vessels, like veins, mitochondrial function remained stable even after 24 h of cold ischemia. HTK
Definition of ischemia time in the Legal Dictionary - by Free online English dictionary and encyclopedia. What is ischemia time? Meaning of ischemia time as a legal term. What does ischemia time mean in law?
Ischemia and reperfusion are natural steps during kidney transplantation, and IRI is considered one of the most important nonspecific factors affecting allograft dysfunction. Transplanted organs experience several episodes of ischemia, in which cold ischemia occurs during allograft storage in preservation solutions. Even though cold ischemia has been studied extensively, all of the studies have been carried out in vitro and ex vivo models. There is no in vivo model available to examine renal IRI induced solely by cold ischemia. In the present study, we developed an in vivo mouse model to study renal IRI induced exclusively by cold ischemia through clamping the renal pedicle for 1 to 5 hours. During the ischemic phase, blood was flushed from the kidney with cold saline through a small opening on the renal vein. The kidney was kept cold in a kidney cup with circulating cooled saline, while the body temperature was maintained at 37℃ during the experiment. The level of kidney injury was evaluated by
Effect of cold ischemic times and time after transplantation on regional myocardial motion after heart transplantation. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Researchers have long considered the NCI supported Cooperative Human Tissue Network (CHTN) as a source for quality collection and preservation of tissue samples from remnant surgical tissue specimens. In an organized tissue procurement setting serving a large population of researchers, the trained procurement personnel, specialized reagents and monitoried frozen storage facilities assure the availability of quality research, monitored preservation techniques and temperature controlled shipping required for successful research support.. The CHTN Midwestern Division (MWD) has experienced an increase in investigator demand for fresh tissues over the past two years. Approximately 23% of the tissues distributed by the CHTN MWD is fresh tissue. Requests for frozen and formlin-fixed paraffin embedded tissues, however, remain constant and stable. The increased interest in fresh tissue likely reflects an increased focus on the study of vital cells in controlled environments for... Read more ...
While the method itself might be close to perfect, upstream (pre-analytic) and downstream (data mining) processes need to be improved.
nurul izzati abdullah zayawi ym:nurizz_fifa105 email:izzati[dot]abdullah[at]gmail[dot]com sek.keb.gong badak [93-99],smka tok jiring [00-04],darul quran jakim [jan05-aug05],cenFOS iium [dec05-sep06],faculty of medicine, mansoura university, egypt [present~] ask me anything here : http://formspring.me/ ...
We previously reported that progression of liver cirrhosis is quicker and survival is dismal in patients with human immunodeficiency virus (HIV) and hepatitis C virus (HCV) coinfection, especially when acquired in childhood through contaminated blood products. Recently, we performed the first deceased donor liver transplantation (DDLT) for an HIV/HCV-coinfected hemophilic patient in Japan. A 40-year-old man was referred to our hospital for liver transplantation. Regular DDLT was performed using the piggyback technique with a full-sized liver graft. Cold ischemia time was 465 min, and the graft liver weighed 1,590 g. The antiretroviral therapy (ART) was switched from darunavir/ritonavir to raltegravir before the transplant for flexible usage of calcineurin inhibitors postoperatively; tenofovir was used as the baseline treatment. The postoperative course was uneventful, and the patient was discharged home on day 43. He started receiving anti-HCV treatment on day 110 with pegylated interferon, ...
Results In the PI arm 11.1% (n=99) had AbMI versus 6.9% (n=59) in primary PCI arm (p,0.01). In a multivariable model, AbMI patients overall had less baseline ΣST-deviation, fewer baseline Q-waves and shorter total ischaemic times. PI AbMI patients had faster time to TNK (90 vs 100 min, p=0.015): total ischaemic time was 100 min longer in primary PCI AbMI patients and no difference in ischaemic time existed between AbMI and non-AbMI patients within this group. Although no significant interaction between treatment and AbMI on the composite endpoint of death/shock/congestive heart failure/recurrent MI occurred (p=0.292), PI AbMI patients had a lower incidence in this endpoint than non-AbMI patients (5.1 vs 12%, p=0.038); this was not evident in primary PCI patients. Forty-five patients (ie, 2.5%) had masquerading MI with minimal biomarker elevation and no evolution in baseline ST-elevation.. ...
Heart transplantation is the final treatment option in heart failure, and transplant patients have substantially increased survival rates and quality of life.27 The first human-to-human heart transplantation was performed in South Africa in 1967, and by the end of 1968 102 patients had received transplants at 52 institutions.1 However, the results were disappointing, with a 60% early death rate and a mean survival of only 29 days.28 In a large study in 2000, when the duration of cold ischaemia was 6 h, graft survival at 3 years after surgery was nearly 25% less than that when the cold ischaemia period was ,2 h.29. Use of organs from marginal donors has resulted in decreased graft function and survival compared with the use of organs from younger donors.30 It is currently difficult to obtain donor hearts. In addition, long-term outcomes for transplanted hearts are limited by the development of cardiac allograft vasculopathy resulting from immunological and non-immunological risk factors ...
Aim: To define regional patterns in post OLT LOS.. Methods: Using the UNOS database (06/2011) we reviewed data on 65,891 OLT recipients, 01/2000-12/2010. Patients excluded (n=11,784) were dual organ, and pediatric recipients, deaths ≤2 days, LOS,180 days or missing data. Variables were categorized (n=54,107): MELD score(≤10, 11-18,19-24, ≥25) Karnofsky score (0-30, 31-60, 61-100), cold ischemia time (CIT, hrs) (,6, 6-12, ,12≤20, ,20). Predictors of LOS were identified univariately, and multivariately using a linear regression model of Log2(LOS). Regional (R) differences in multivariate associations were assessed using parameters for interactions with region, and within regions. The LOS median ratio (MR) was used as a measure of relative change in median LOS.. Results: Mean LOS ranged from 13.3±14(Mean±SD) days in R6 to 19.5±20 days in R9. On multivariate analysis factors associated with ,10% increase LOS were: patient ventilated at OLT (LOS MR estimate 1.33, i.e. 33% increase in LOS), ...
3000 mg/g: ~1% in both). Incidence of PU as an AE was 14.1% (EVR+rCNI) and 7.0% (MPA+sCNI). Median time to onset was 76 vs 63 days with EVR+rCNI vs MPA+sCNI. Drug discontinuation due to PU AE was noted in 26 vs 0 patients in EVR+rCNI vs MPA+sCNI (Table). Among RTxRs with PU, baseline demographics were mostly balanced, except for higher proportion of RTxRs with cold ischemia time >20 h in EVR+rCNI vs MPA+sCNI arm (18.9% vs 9.9%). In patients with PU AE, though mean EVR C0 was within target range from M1-M6, it was slightly lower in patients who continued (5.4-6.1 ng/mL) vs those who discontinued (5.9-7.0 ng/mL). Between-arm difference in median UPCR up to M24 was 2.5-fold higher in EVR+rCNI vs MPA+sCNI arm among patients with PU. Median UPCR of patients discontinuing study drug was higher than that of those continuing study drug in both arms. Use of loop diuretics was more frequent in EVR+rCNI vs MPA+sCNI arm.. *Conclusions: Although EVR+rCNI regimen was associated with a high incidence of PU ...
Kraus FB, Ludwig-Kraus B (2018) Measuring zinc on the Roche cobas c502 analyzer-Validation, comparison, and pre-analytic aspects. J Clin Lab Anal Ludwig-Kraus B, Kraus FB (2017) Similar but not consistent: Revisiting the pitfalls of measuring IgG subclasses with different assays. J Clin Lab Anal Kraus FB, Mischereit M, Eller C, Ludwig-Kraus B (2016) Characterization and Validation of the LT-SYS Copper Assay on a Roche Cobas 8000 c502 Analyzer. Laboratory Medicine, lmw049. Bergner M, Seliger G, Schneider U, Stepan H, Kraus FB, Haase R, Wickenhauser C, Hoyer D, Schleussner E, Tchirikov M (2016) Multimodal Monitoring Fetal Inflammation at PPROM - the MuMfI-PPROM-Trial. Geburtshilfe Frauenheilkd. 76- P18. Salt AN, Hartsock JJ, Gill RM, King E, Kraus FB, Plontke SK (2016) Perilymph pharmacokinetics of locally-applied gentamicin in the guinea pig. Hearing Research 342, 101-111. Kapheim KM et al. (2015) Genomic signatures of evolutionary transitions from solitary to group living. Science 348, 1139-1143. ...
Histological Analysis At euthanization, renal tissue specimens were fixed Animal weights did not differ between the groups in neutral 10% buffered formalin and embedded in (MP25, 23.5 ± 4.6 kg; MP30, 26.3 ± 3.2 kg; p = paraffin. Tissue injury was assessed on periodic acid- 0.201). All animals demonstrated normal renal func- Schiff (PAS) stained 4 lm-thick sections by scoring tion before the start of the experiments (serum creati- glomerular damage (shrinking), tubular damage, infil- nine; MP25, 1.09 ± 0.14 mg/dL; MP30, 1.13 ± 0.10 tration of inflammatory cells, interstitial edema and mg/dL; p = 0.565). Cold ischemic times (CITs) necrosis. Tubular damage was defined as dilatation, of the groups were comparable (CIT, MP25, 20:32 h ± denudation of the tubular basement membrane and 0:06; MP30, 20:28 h ± 0:17; p = 0.158). Also, times vacuolization. Injury of each specimen was graded to required for both anastomoses were comparable the extent of region involvement in 10 randomly cho- between the ...
Citáty Ben Bernanke. Přehled nejoblíbenějších a nejznámějších citátů. Ben Shalom Bernanke je americký ekonom, bývalý předseda rady guvernérů Federál…
During the day, I get hot unexpectedly. I didnt care for the cold to begin with but now being in a room thats too cool will bother me. At night, it just gets weird. Ill be hot then cold, then hot, then cold. I have had the fan on and the covers over me within a minute of each other - and more than once a night! So, is this like normal? :huh:
Background. In renal transplantation, cold ischaemia (CI) determines acute rejection through innate immunity among others. Acute rejection episodes are a risk factor for late allograft dysfunction and proteinuria. This implies some alteration of the glomerular filtration barrier (GFB). Besides its effects on acute rejection, we hypothesized that CI might somehow damage the GFB being directly responsible for late proteinuria.. Methods. On rat kidney allografts suffering from antibody-mediated acute rejection with or without CI and compared with syngeneic grafts, we quantified the gene expression of innate and adaptive immune mediators and assessed the capillary glomerular basement membranes (CapBM) by immunostaining collagen-IV (ColIV). ColIV was also assessed in equivalent groups from a previous chronic study followed up for 24 weeks.. Results. CI up-regulated enzymes critical in the stabilization of collagen chains, increasing ColIV deposition and thickening the CapBM. CI increased the C4d and ...
Adhesion molecules play a crucial role in transplant rejection in regulating the interaction of inflammatory cells with cells in the vascular wall. In an aortic transplantation model, we have previously analysed the early adhesion process (7.5 min to 24 h) and the impact of cold ischaemia time (1-24 h) upon transplant arteriosclerosis during the first 2 months after transplantation in the rat. The aim of this investigation was to study adhesion molecules in accelerated transplant arteriosclerosis in a rat model by analysing the immunohistochemical expression of CD11b and ICAM-1 up to 2 months and followed by a semiquantitative evaluation and multivariant analysis. Antigen expression of CD11b and ICAM-1 adhesion molecules was stronger in the aortic allografts than in the ischaemia-induced syngeneic aortic grafts in the whole vessel wall. Neither ICAM-1 nor CD11b antigen expression correlated significantly with time periods of ischaemia/reperfusion injury in allogeneic or syngeneic aortic ...
Date: 19 Mar 96 00:18:15 EST From: Mike Darwin ,. , Subject: BPI TECH BRIEF #18 Cryopreservation of CryoCare Patient #C-2150 by Mike Darwin Introduction On December 12th, 1995 James Gallagher, a 55-year-old software developer from Sunset Beach, California, became CryoCares first member to enter cryopreservation. He also became the first patient ever to benefit from new technologies developed to reduce three forms of injury: * pre-mortem shock * warm ischemia (the time interval between pronouncement of death and restoration of adequate blood circulation) * cold ischemia experienced during initial blood washout and cooling, and also during iced-transport from the location where legal death occurred to the facility where cryoprotective perfusion is carried out. The following is not quite a full technical report, but neither is it simply a lay-level of summary of key events without reference to the technical details and the impact those details had upon this patients care and potentially, future ...
Chill out with our top seven low-potassium fruit salads for a kidney diet. It may be hot outside, but you can keep cool with recipes such as Caramel Apple Salad or Festive Fruit Salad.
Whole grains are high in protein and potassium, with the exception of oatmeal. Processed grains in products such as pasta, bread, bagels and cereal are lower in potassium and suitable for a low-potassium diet. Avoid processed foods that have trans fats, such as crackers and cookies.. ...
Description, comparison with clinical data and clinical exploitation of a non-invasive and relatively inexpensive method for screening cardiovascular systems based on multi-parameter analysis of heart haemodynamics and vascular perfusion in selected areas of the body ...
Read 9 responses to: I have a 8 month old baby and she has had a cold for... Find the best answer on Mamapedia - mom trusted since 2006.
Ischemia-reperfusion (I/R) associated with hepatic resection and living related liver transplantation is an unsolved problem in clinical practice. Indeed, I/R induces damage and regenerative failure in clinical liver surgery. Signaling pathways regarding the pathophysiology of liver I/R and regeneration making clear distinction between situations of cold and warm ischemia, as well as liver regeneration with or without vascular occlusion, will be addressed. The different experimental models used to date to improve the postoperative outcomes in clinical liver surgery will be also described. Furthermore, the most updated therapeutic strategies, as well as the clinical and scientific controversies in the field, will be discussed. Such information may be useful to guide the design of better experimental models as well as the effective therapeutic strategies in liver surgery that can succeed in achieving its clinical application.
University of Maryland School of Medicine Background: A full facial procurement should allow concurrent procurement of all solid organs and ensure their integrity. Procurement of facial vascularized composite allografts (VCA) challenges the surgical teams with complex defects potentially requiring far greater time than solid organ procurement. Simultaneous-start procurement in these cases could entail cold ischemia times over 12 hours, risking post-reperfusion bleeding and reperfusion injury.. Methods: A VCA including all facial skin, mimetic muscles, multiple sensory and motor nerve branches, anterior tongue, and maxillary and mandibular segments was procured from a brain dead donor. Solid organ recovery included heart, lungs, liver, kidneys, and pancreas. Bedside tracheostomy and facial mask impression were performed the day before procurement.. Results: Facial VCA dissection time was 12 hours, spaced over a 15 hour period to diminish ischemia while the recipient was being prepared (Fig 1). ...
The incidence of primary graft dysfunction (PDF) after LT is approximately 25% within a range of 9.3 to 43.7% based on different definitions characterizing PDF [1-5]. Initial non-function (INF) develops in up to 6% of all considered cases and requires urgent retransplantation [6].. Risk factors are prolonged cold ischemia time or higher degree of graft steatosis and subsequent development of severe hepatic ischemia/reperfusion injury (IRI) [5, 7]. A cascade of cellular events results in microcirculatory flow disturbance. This may be distinguished as "no-flow" indicating capillary perfusion failure on the one hand, or "reflow-paradox" including activation of the leukocyte-endothelium interaction, release of toxic mediators, and impairment of the endothelial barrier on the other hand [8, 9]. There are, obviously, further different factors all contributing to an insufficient function of liver grafts and its detrimental effects on the overall outcome after LT: recipients condition, the donor data, ...
TY - JOUR. T1 - Az ischaemiás posztkondicionálás hatása az oxidatív stresszre és a szöveti struktúrára vékonybél meleg ischaemiás és autotranszplantációs modellekben.. AU - Nedvig, Klára. AU - Völgyi, Edina. AU - Wéber, G.. AU - Rőth, E.. AU - Ferencz, A.. PY - 2011/12. Y1 - 2011/12. N2 - Our study investigated the effect of ischemic postconditioning (IPO) in intestinal warm ischemia/reperfusion (I/R) and autotransplantation models. Warm ischemia was performed by occlusion of superior mesenteric artery for 1, 3 and 6 hours in white domestic pigs (n = 15). Prior to 3 hours reperfusion the intestine was postconditioned by 3 cycles of 30-seconds ischemia and 30-seconds reperfusion (IPO protocol). In the cold ischemia group (n = 15) the bowel was preserved in University of Wisconsin solution for 1, 3, and 6 hours. Prior to 3 hours reperfusion IPO protocol was applied, too. Tissue samples were collected after laparotomy (control) and at the end of the reperfusion periods. As far as ...
Donation after cardiac death (DCD) donors are vital to maximize the organ donor pool. Reperfusion injury (RI) is an important sequela in DCD organs due to warm and cold ischemia. RI manifests clinically as a high incidence of delayed graft function (DGF) and primary non-function (PNF) compared with donation after brain death organs. The importance of nitric oxide (NO) in the generation of reperfusion injury is pivotal ...
The research report titled Global Organ Preservation Solutions Market Professional Survey Report 2017 market size and forecast and overview on current market trends
I have a cold storage room underneath my front porch. It is poured out of concrete. It is around 20 long wide by 5 feet Wide. We dont use it at all for cold storage, but want to use it for regular storage year round. Obviously it is very cold in there during the winter.
Magnetic Particle Imaging of the vasculature to assess blood delivery and tissue level perfusion. The direct quantitation of nanoparticle tracers enables accurate perfusion measurements without confounding background tissue signal. Long circulating tracers persist within the vasculature for days, enabling repeated measurements for longitudinal studies. This enables truly functional imaging to measure changes in blood flow and tracer delivery over time in stroke and tumor models. ...
Colds and Flu - Dos & Donts There are beneficial procedures for treating colds and flu that are easily overlooked. Meanwhile, a lot of commonly
Cold and flu can be extremely contagious and are usually more common at certain parts of the year. Learn about cold and flu prevention and treatment.
Whether you have a cold or the flu, our helpful guide will give you tips on how to fight the symptoms. Learn how to get better sooner.
People think the perfect day for running has bluebird skies and a slight breeze. But cold, rainy runs are key to unlocking potential and mental grit.
Just wondering if anyone else gets cold while on this diet? I have been sole sourcing for approx 6 weeks and my hands are freezing! This isnt the...
Read 9 responses to: Has anyone had a severe cold while being pregnant? ..... Find the best answer on Mamapedia - mom trusted since 2006.
Optimal lung preservation via flush of the pulmonary vasculature minimizes early graft failure post-lung transplantation. We hypothesized that the route of pulmonary flush has differential effects on thermal gradients in the lung and expression of inflammatory mediators. Swine underwent antegrade flush (AG) via pulmonary artery; AG/RG: antegrade + retrograde flush via pulmonary veins or AG/BA: antegrade + bronchial artery flush via bronchial artery. Temperatures were recorded in bronchial mucosa and peribronchial lymph nodes. RT-PCR was utilized to detect cytokine gene expression in the nodes. AG/BA flush resulted in greatest cooling of bronchial mucosa and lymph nodes (p < 0.001). The route of flush did not affect expression of RANTES, MCP-1, IL-8, IL-1, TNF-α or IL-6. However, expression of Gro was reduced 4-h post-preservation in all groups. Only AG/BA resulted in decreased IFN- transcripts. These data show that, compared to AG or AG/RG, AG/BA flush results in the greatest cooling of ...
TY - JOUR. T1 - cAMP-mediated vascular protection in an orthotopic rat lung transplant model. T2 - Insights into the mechanism of action of prostaglandin E1 to improve lung preservation. AU - Naka, Yoshifumi. AU - Roy, Dilip K.. AU - Liao, Hui. AU - Chowdhury, Nepal C.. AU - Michler, Robert E.. AU - Oz, Mehmet C.. AU - Pinsky, David J.. PY - 1996. Y1 - 1996. N2 - Prostaglandin E1 (PGE1) is often added to the donor pulmonary flush solution to enhance clinical lung preservation for transplantation. Although PGE1 is thought to act as a pulmonary vasodilator during the harvest period, the precise mechanism(s) of action whereby PGE1 enhances lung preservation is unknown. Because cAMP levels decline in endothelial and vascular smooth muscle cells exposed to hypoxia, we hypothesized that a PGE1-mediated increase in cAMP levels within the preserved lungs might improve pulmonary vascular homeostasis following lung transplantation. Rat lungs demonstrated a time-dependent decline in cAMP levels during ...
Damage of the biliary tract at the end of a period of hepatic preservation prior to liver transplantation has been observed in man. This damage may be important in the development of early biliary complications. A model of hepatic biliary ischaemia in the pig has been developed which permits comparison between the influences of warm and cold ischaemic damage on the biliary tract. The major damage to the biliary tract in the pig in this model appears to occur after prolonged cold preservation.
This test does not include a pathology consultation. If a pathology consultation is requested, PATHC / Pathology Consultation should be ordered and the appropriate FISH test will be ordered and performed at an additional charge. Mayo Hematopathology Consultants are involved in both the pre-analytic (tissue adequacy and probe selection, when applicable) and post-analytic (interpretation of FISH results in context of specific case, when applicable) phases.. This test includes a charge for application of the first probe set (2 FISH probes) and professional interpretation of results.. Additional charges will be incurred for all reflex probes performed. Analysis charges will be incurred based on the number of cells analyzed per probe set. If no cells are available for analysis, no analysis charges will be incurred.. Depending on the lymphoma subtype suspected, the most appropriate probes to order are listed in the Clinical Information.. If the patient is being tracked for known abnormalities, ...
The benefit of adding Sodium Nitroprusside (NPNa) or S-nitrosolutathion (GSNO) to the university of Wisconsin solution (UW) to prevent morphological alterations during cold preservation / reperfusion of rat livers ...
More than 200 viruses can cause colds. Because there are so many, there isnt a vaccination to prevent you from getting colds. Fortunately, your body already has the best cold cure - your immune system.
With human colds being so common, its natural to wonder whether our dogs can catch colds too. Here is everything you need to know about whether or not dogs can catch colds.
Answers for Can you get the same cold twice:If youre talking about the common cold, then the answer is NO, you cannot catch the same cold twice. Thanks for using ChaCha!
Can the cold and flu be eliminated, or better yet prevented, without medication? Lets just say, avoiding the cold and flu is akin to preparing a dish before your guests arrive.
Beechams Cold Relief is a medicine available in a number of countries worldwide. A list of US medications equivalent to Beechams Cold Relief is available on the Drugs.com website.
28 yrs old Male asked about Cough and cold, 1 doctor answered this and 172 people found it useful. Get your query answered 24*7 only on | Practo Consult
Hi, its been years I use nosodes to prevent colds with my little daughter. They do usually work if the cold is caught in the first day or very early. I use ene
Night skiing tonight. Man is it ever gonna be cold. Ill have more layers than grandmas lasagne. -31 at Mount Tremblant. -13 at Whiteface -15 at Jay -16 Killington I have skiied this and lots colder. But is it ever too cold? Whos hardcore?
Because sneezing and coughing are |i|not|/i| how you want to spend your day The typical adult averages two to three colds a year, according to the Centers for Disease Control. And whether youve already topped that number or youve somehow stayed congestion-free this season, youd probably try pretty much anything that promises to stop a…
Had a cold, months ago, and I got better, but the coughs stuck. (its been 2 months now) still coughing. Non of the cough medicine works, doctor prescribed antibiotic, which also did nothing. Another d...
The immune system is divided into the innate and adaptive immune responses. The innate immune response is known as the first line of defense, and it depends mostly on inflammatory components. It is faster and less specific than the adaptive response. In contrast, the adaptive response involves the participation of lymphocytes, and it generates memory. It takes longer to build an adaptive response, but such responses are more specific than innate responses. While adaptive immune responses are an excellent system for fighting pathogens, they are also very effective against allograft acceptance. In solid organ transplantation, the graft is subjected to ischemia prior to being transplanted. Ischemia and reperfusion (IR) is the first step in which the immune system acts to avoid the survival of the graft. Ischemia is defined as the cessation of arterial blood flow, which leads to oxygen deprivation of the cells. Cold ischemia is most often used in transplantation, whereby the organ is harvested and ...
View Poster. INTRODUCTION. Tumor enucleation (TE) optimizes parenchymal preservation and could yield better function than standard partial nephrectomy (SPN). However, data about this are controversial. Our study compares functional outcomes for TE and SPN strategies.. METHODS. Patients managed with partial nephrectomy (PN) with necessary data for analysis of preservation of ipsilateral parenchymal mass and global GFR from two centers were included. All studies were required RESULTS. Analysis included 71 TE and 373 SPN. Median preoperative global GFR was comparable for TE and SPN (75mL/min/1.73m2 versus 78mL/min/1.73m2, p=0.6). Median tumor size was 3.0cm for TE and 3.3cm for SPN (p=0.03). Median R.E.N.A.L scores were 7 in both cohorts. For TE, warm and zero ischemia were used in 51% and 49%, respectively. For SPN, warm and cold ischemia were used in 72% and 28%, respectively. Capsular closure was performed in 46% of TE and 100% of SPN (p. CONCLUSION. Our analysis suggests that TE has potential ...
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More than 200 viruses can cause colds. Because there are so many, there isnt a vaccination to prevent you from getting colds. Fortunately, your body already has the best cold cure - your immune system.
First Cold - My little Kylie got her first cold recently (she is 8 months old). Mostly she just has a lot of mucus draining from her nose. She...
cough and cold - I have a son 5 year old and a daughter 3 month old . Both of have heavy cough and cold from last 1 month. Doctor have given...
The common cold is truly common -- the most contagious infection youll encounter. In this section, weve separated fact from myth. Learn to prevent nasty colds.
TY - JOUR. T1 - Long-term lung preservation with the PAF antagonist BN 52021. AU - Conte, John. AU - Katz, Nevin M.. AU - Wallace, Robert B.. AU - Foegh, Marie L.. PY - 1991/1/1. Y1 - 1991/1/1. N2 - Platelet activating factor (PAF, l-alkyl-2(R)-acetyl- glycero-3-phosphorylcholine) is a phospholipid that is released by a variety of cells. The similarity between the pathophysiological effects of PAF and posttrans-plant pulmonary dysfunction led to an evaluation of a PAF antagonist as an adjunct to lung preservation. The ginkgolide B, BN 52021, was selected as the PAF antagonist to be studied because of the large data base available on this compound. BN 52021 was given to the donor and recipient (10 mg/kg i.v.) prior to harvest and transplantation and was included in 1 L of preservation solution (10 mg/kg) used for flushing the pulmonary artery and for storage. Left single-lung transplantation was performed following a 22-hr preservation period at 10°C. Arterial oxygen tension (pO2), pulmonary ...
TY - JOUR. T1 - Optimizing the concentration of hydroxyethylstarch in a novel intestinal-specific preservation solution. AU - Kokotilo, Matthew S.. AU - Carter, Jodi. AU - Thiesen, Aducio. AU - Chen, Ming H.. AU - Ochs, Angela. AU - Khadaroo, Rachel G.. AU - Churchill, Thomas A.. PY - 2010/12/1. Y1 - 2010/12/1. N2 - Introduction: Our lab has developed an effective nutrient-rich solution that facilitates energy production and control of oxidative stress during static cold storage of the intestine; however, the requirement for oncotic agents, such as hydroxyethylstarch (HES), has not been evaluated. This study investigated the effectiveness and requirement for HES in an intraluminal preservation solution during a clinically relevant period of cold storage. Methods: Rat intestines were procured, including an intravascular flush with University of Wisconsin solution followed by a back table intraluminal flush with a nutrient-rich preservation solution containing varying amounts of HES ( n= 6 per ...
Commercial. The Cold Storage Equipment market competitive factors leading to heavy market demand and development scope is analyzed thoroughly. Also, a special focus on distributors, manufacturers, traders, and suppliers is provided. The 5-year forecast industry scenario will gauge the Global Cold Storage Equipment industry potential. The market value, volume and consumption forecast by region, type and application is portrayed in the study. Up-to-date qualitative and quantitative market aspects with sales numbers are presented. Also, the detailed analysis of past performance of Global Cold Storage Equipment market and business-related moves with the present scenario is studied.. Main Highlights Of the Global Cold Storage Equipment Market Report:. 1. The report offers an analytical study on various global Cold Storage Equipment industries to provide decisive data.. 2. The top market segment analysis and Global Cold Storage Equipment market drivers are explained in this report.. 3. A mix of ...
Cold sore symptoms? If you are curious, read this article and discover what you should know and expect from your cold sore symptoms.
|p|If you have a cold, you know how much misery it can bring. Read on to learn how to get rid of your cold as quickly as possible.|/p|
Magic Forum: Cold Healing Herbs - So, my mum has a cold and its very bad, she can hardly talk, sneezing and coughing all the time is....
in Acta Gastro-Enterologica Belgica (2014, March), 77(1), 16. Aim: Results of donation after circulatory death (DCD) liver transplantation (LT) are impaired by ischemic bile duct lesions caused by procurement warm ischemia. Donor age is a risk factor in deceased ... [more ▼]. Aim: Results of donation after circulatory death (DCD) liver transplantation (LT) are impaired by ischemic bile duct lesions caused by procurement warm ischemia. Donor age is a risk factor in deceased donor LT, and particularly in DCD-LT. At the authors institute, age is not an absolute exclusion criterion to discard DCD liver grafts, controlled DCD donors receive comfort therapy before withdrawal, and cold ischemia is minimized. The aim of the present study was to report on the results of the first 10 years of this experience, and particularly on graft survival and the rate of post-transplant biliary complications, according to DCD donor age. Methods: The authors retrospectively studied a consecutive series of 70 DCD-LT ...
Disclosed is a rinse for preservation solution for organs and tissues selected for transplantation. The solution can be used at all temperatures from 0 C. to normal body temperature and continues to be protective above 10 C. Methods for preparing organs and tissues preserved in a preservation solution prior to transplant into a recipient are also disclosed.
Company profile & key executives for United States Cold Storage Inc (1013626D:-) including description, corporate address, management team and contact info.
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Dear Readers, this week I have been suffering from a rather pernicious head cold, which has laid me low and prevented me from exploring my half-mile territory. A commonplace experience (the average adult will suffer from two to four colds every year), but it got me thinking. What causes a cold? How do our typical…
With almost a third (31 per cent) of all sick days taken in the UK blamed on the dreaded cold virus, it is more than likely that you missed a day of work in the last 12 months as a result of the common cold, according to a new survey by herbal cold remedy, Kaloba.…
Looking for the best ThermaCare cold & hot pack? Read unbiased ThermaCare cold & hot pack reviews and find the top-rated ThermaCare cold & hot packs.
To feel healthy during cold and flu season, prevent a cold from taking over your body in the first place. Here, doctors explain the best ways to avoid getting sick.
Typically, one cold sore will progress through 5 stages throughout a 7 - 10 day process. Knowing the cold sore stages stages can help you be prepared. Learn more.
Truman Capotes classic In Cold Blood was not in cold storage for long after the rights for the book reverted back to the Truman Capote Literary Trust.
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Feeding time - My baby has a cold: Top treatments for poorly babies - You might find that your baby goes off food when theyve got a cold. Its pretty common, but its important to keep on offering them food and drink regularly. Lisa says you...
Magyarorsz gon igen j az toltotts gi ar ny,k sz nhet en a k telez v d olt soknak. A leggyakrabban el fordul vagy nagyon s lyos sz v dm nyekkel j r betegs gek ellen k telez en oltani kell,de vannak olyan olt sok,amelyek nem k telez ek csak aj nlottak.
Cold sweats are often associated with your bodys fight or flight response. Heres what may be causing your symptoms and how to treat them.
Many watchers of the presidential debate noted that Donald Trump kept sniffling. He said he didnt have a cold, so what else might have caused the sniffling?

Ex Vivo Transfer of Nuclear Factor-κB Decoy Ameliorates Hepatic Cold Ischemia/Reperfusion Injury<...Ex Vivo Transfer of Nuclear Factor-κB Decoy Ameliorates Hepatic Cold Ischemia/Reperfusion Injury<...

Yoshizumi T, Ikeda Y, Kaneda Y, Sueishi K. Ex Vivo Transfer of Nuclear Factor-κB Decoy Ameliorates Hepatic Cold Ischemia/ ... N2 - Cold ischemia/reperfusion injury of the hepatic graft has been attributed to the release of various inflammatory cytokines ... AB - Cold ischemia/reperfusion injury of the hepatic graft has been attributed to the release of various inflammatory cytokines ... Cold ischemia/reperfusion injury of the hepatic graft has been attributed to the release of various inflammatory cytokines. ...
more infohttps://kyushu-u.pure.elsevier.com/en/publications/ex-vivo-transfer-of-nuclear-factor-%CE%BAb-decoy-ameliorates-hepatic-c

Living-related liver transplantation in children: The Parisian strategy to safely increase organ availability<...Living-related liver transplantation in children: The 'Parisian' strategy to safely increase organ availability<...

Mean overall cold ischemia time averaged 140 minutes (range, 90 to 230 minutes). Twenty-four of 26 patients had end-to-end ... Mean overall cold ischemia time averaged 140 minutes (range, 90 to 230 minutes). Twenty-four of 26 patients had end-to-end ... Mean overall cold ischemia time averaged 140 minutes (range, 90 to 230 minutes). Twenty-four of 26 patients had end-to-end ... Mean overall cold ischemia time averaged 140 minutes (range, 90 to 230 minutes). Twenty-four of 26 patients had end-to-end ...
more infohttps://einstein.pure.elsevier.com/en/publications/living-related-liver-transplantation-in-children-the-parisian-str-2

Cold Ischemia During Surgery - Semantic ScholarCold Ischemia During Surgery - Semantic Scholar

Cold Ischemia During Surgery. Known as: cold ischemia In surgery, the cooling of a tissue, organ, or body part after its blood ... Laparoscopic partial nephrectomy in cold ischemia: renal artery perfusion.. *Gunter Janetschek, Alaa Eldin A. Abdelmaksoud, Dr ... We examined the effects of upregulation of heme oxygenase-1 (HO-1) in steatotic rat liver models of ex vivo cold ischemia… ( ... BACKGROUND Prolonged cold ischemia time (CIT) is accompanied by delayed cadaveric renal allograft function and early allograft ...
more infohttps://www.semanticscholar.org/topic/Cold-Ischemia-During-Surgery/155546

Hepatic Regeneration Under Warm or Cold Ischemia Conditions: Controversies and New Approaches | IntechOpenHepatic Regeneration Under Warm or Cold Ischemia Conditions: Controversies and New Approaches | IntechOpen

... the pathophysiology of liver I/R and regeneration making clear distinction between situations of cold and warm ischemia, as ... Ischemia-reperfusion (I/R) associated with hepatic resection and living related liver transplantation is an unsolved problem in ... type of ischemia applied (warm or cold), period of ischemia (ranging from minutes to days), extension of hepatic ischemia ( ... 4. Controversies on hepatic regeneration under warm or cold ischemia. In an attempt to expand the size of the donor pool, the ...
more infohttps://www.intechopen.com/books/surgical-challenges-in-the-management-of-liver-disease/hepatic-regeneration-under-warm-or-cold-ischemia-conditions-controversies-and-new-approaches

The effects of death and post-mortem cold ischemia on human tissue transcriptomesThe effects of death and post-mortem cold ischemia on human tissue transcriptomes

CitacióFerreira, P., Muñoz, M., Reverter Comes, F. The effects of death and post-mortem cold ischemia on human tissue ... The effects of death and post-mortem cold ischemia on human tissue transcriptomes. ...
more infohttps://upcommons.upc.edu/handle/2117/115587

IJMS  | Free Full-Text | Impact of Cold Ischemia on Mitochondrial Function in  Porcine Hearts and Blood Vessels | NotesIJMS | Free Full-Text | Impact of Cold Ischemia on Mitochondrial Function in Porcine Hearts and Blood Vessels | Notes

Hearts, saphenous veins, internal-mammary-arteries and aortas of male landrace pigs were harvested and exposed to cold ischemia ... HTK-Custodiol protection of mitochondrial function after prolonged cold ischemia was observed in the myocardium but not in ... mitochondrial function remained stable even after 24 h of cold ischemia. ... Analysis of mitochondrial function can be used as a valuable approach for the assessment of cold ischemic injury in various ...
more infohttp://www.mdpi.com/1422-0067/14/11/22042/notes

CAN EFFLUENT HYALURONIC ACID OR CREATINE KINASE PREDICT SINUSOIDAL INJURY SEVERITY AFTER COLD ISCHEMIA?CAN EFFLUENT HYALURONIC ACID OR CREATINE KINASE PREDICT SINUSOIDAL INJURY SEVERITY AFTER COLD ISCHEMIA?

The cold ischemia time (CIT) was recorded. Statistical comparisons were made among HA level, CK-BB level, CIT, and graft ... CAN EFFLUENT HYALURONIC ACID OR CREATINE KINASE PREDICT SINUSOIDAL INJURY SEVERITY AFTER COLD ISCHEMIA?. ...
more infohttps://insights.ovid.com/trans/199312000/00007890-199312000-00009

Effects of warm and cold ischemia on mitochondrial functions in brain, liver and kidney | SpringerLinkEffects of warm and cold ischemia on mitochondrial functions in brain, liver and kidney | SpringerLink

... and cold (4°C) ischemia on different mitochondrial functions in rat brain, liver ... Baumann, M., Bender, E., Sömmer, G. et al. Effects of warm and cold ischemia on mitochondrial functions in brain, liver and ... Effects of warm and cold ischemia on mitochondrial functions in brain, liver and kidney. *Matthias Baumann1. , ... The purpose of this work was to study the effects of warm (37°C) and cold (4°C) ischemia on different mitochondrial functions ...
more infohttps://rd.springer.com/article/10.1007%2FBF00219256

cold ischemia | CHTN Blogcold ischemia | CHTN Blog

Researchers have long considered the NCI supported Cooperative Human Tissue Network (CHTN) as a source for quality collection and preservation of tissue samples from remnant surgical tissue specimens. In an organized tissue procurement setting serving a large population of researchers, the trained procurement personnel, specialized reagents and monitoried frozen storage facilities assure the availability of quality research, monitored preservation techniques and temperature controlled shipping required for successful research support.. The CHTN Midwestern Division (MWD) has experienced an increase in investigator demand for fresh tissues over the past two years. Approximately 23% of the tissues distributed by the CHTN MWD is fresh tissue. Requests for frozen and formlin-fixed paraffin embedded tissues, however, remain constant and stable. The increased interest in fresh tissue likely reflects an increased focus on the study of vital cells in controlled environments for... Read more ...
more infohttps://www.chtn.org/blog/taxonomy/term/19

Cold ischemia time | Article about cold ischemia time by The Free DictionaryCold ischemia time | Article about cold ischemia time by The Free Dictionary

The concept of time may be discussed on several different... Explanation of cold ischemia time ... Find out information about cold ischemia time. sequential arrangement of all events, or the interval between two events in such ... redirected from cold ischemia time). Also found in: Dictionary, Thesaurus, Medical, Legal.. Related to cold ischemia time: warm ... Cold ischemia time , Article about cold ischemia time by The Free Dictionary https://encyclopedia2.thefreedictionary.com/cold+ ...
more infohttp://encyclopedia2.thefreedictionary.com/cold+ischemia+time

Cold Ischaemia Expert for Consulting, Expert WitnessCold Ischaemia Expert for Consulting, Expert Witness

Experts available for cold ischaemia consulting, expert witness, or analyst services for business, legal, and technical ... cold ischaemia Experts. To improve your results, click a Related Expert Area on the left.. ... Join our distinguished experts for cold ischaemia jobs, projects and consulting opportunities. ...
more infohttp://www1.orcexperts.com/experts.asp?strSearchType=all&strQuery=cold+ischaemia

PARP Inhibition Attenuates Histopathological Lesion in Ischemia/Reperfusion Renal Mouse Model after Cold Prolonged IschemiaPARP Inhibition Attenuates Histopathological Lesion in Ischemia/Reperfusion Renal Mouse Model after Cold Prolonged Ischemia

i,Results,/i,. Prolonged cold ischemia time in UW solution increased PARP-1 expression and kidney injury. Preconditioning with ... In conclusion, PARP inhibition attenuates ATN and other IR-related renal lesions in mouse kidneys under prolonged cold storage ... If confirmed, these data suggest that pharmacological manipulation of PARP activity may have salutary effects in cold-stored ... and other renal lesions related to prolonged cold ischemia/reperfusion (IR) in kidneys preserved at 4°C in University of ...
more infohttps://www.hindawi.com/journals/tswj/2013/486574/

PARP Inhibition Attenuates Histopathological Lesion in Ischemia/Reperfusion Renal Mouse Model after Cold Prolonged IschemiaPARP Inhibition Attenuates Histopathological Lesion in Ischemia/Reperfusion Renal Mouse Model after Cold Prolonged Ischemia

... ... Nathalie Le Clef, Anja Verhulst, Patrick C. DHaese, and Benjamin A. Vervaet, "Unilateral Renal Ischemia-Reperfusion as a ... "Effect of Γ-aminobutyric acid on kidney injury induced by renal ischemia-reperfusion in male and female rats: Gender-related ...
more infohttps://www.hindawi.com/journals/tswj/2013/486574/citations/

DI-fusion OKT3 prophylaxis in Renal Grafts with prolonged cold ischemia...DI-fusion OKT3 prophylaxis in Renal Grafts with prolonged cold ischemia...

OKT3 prophylaxis in Renal Grafts with prolonged cold ischemia times: association with improvement in long term survival. ... OKT3 prophylaxis in Renal Grafts with prolonged cold ischemia times: association with improvement in long term survival ...
more infohttps://difusion.ulb.ac.be/vufind/Record/ULB-DIPOT:oai:dipot.ulb.ac.be:2013/15215/Details

Influence of Cold Ischemia Time in Kidney Transplants From Small Pediatric DonorsInfluence of Cold Ischemia Time in Kidney Transplants From Small Pediatric Donors

Clinicians may be reluctant to transplant small pediatric kidneys that have prolonged cold ischemia time (CIT) for fear of an ... Influence of Cold Ischemia Time in Kidney Transplants From Small Pediatric Donors. ... additional deleterious effect because pediatric grafts are thought to be more sensitive to ischemia. We aimed to assess the ...
more infohttps://insights.ovid.com/pubmed?PMID=28706987

Cold ischaemia, innate immunity and deterioration of the glomerular filtration barrier in antibody-mediated acute rejection :...Cold ischaemia, innate immunity and deterioration of the glomerular filtration barrier in antibody-mediated acute rejection :...

In renal transplantation, cold ischaemia (CI) determines acute rejection through innate immunity among others. Acute rejection ... In renal transplantation, cold ischaemia (CI) determines acute rejection through innate immunity among others. Acute rejection ...
more infohttp://oxfordindex.oup.com/view/10.1093/ndt/gfs003

The effects of death and post-mortem cold ischemia on human tissue transcriptomes. | Clinical Bioinformatics AreaThe effects of death and post-mortem cold ischemia on human tissue transcriptomes. | Clinical Bioinformatics Area

Blood; Cold Ischemia; Death; Female; gene expression; Humans; Models, Biological; Postmortem Changes; RNA, Messenger; ... The effects of death and post-mortem cold ischemia on human tissue transcriptomes.. ... The effects of death and post-mortem cold ischemia on human tissue transcriptomes.. ...
more infohttp://clinbioinfosspa.es/content/effects-death-and-post-mortem-cold-ischemia-human-tissue-transcriptomes

Cold ischemia time and allograft outcomes in live donor renal transplantation: Is live donor organ transport feasible?Cold ischemia time and allograft outcomes in live donor renal transplantation: Is live donor organ transport feasible?

... Academic ... While transport of the kidney is an attractive alternative, there is concern that prolonged cold ischemia time (CIT) would ...
more infohttp://scholars.uab.edu/display/pub1245731

Cold Ischemia And Renal Graft Survival: A Paired Analysis Comparing First And Second Grafts From The Same Donor. - Medbrary.com...Cold Ischemia And Renal Graft Survival: A Paired Analysis Comparing First And Second Grafts From The Same Donor. - Medbrary.com...

... with special interest in cold ischemia times (CIT) as a risk factor for graft survival. METHODS: We retrospectively reviewed ... Cold Ischemia And Renal Graft Survival: A Paired Analysis Comparing First And Second Grafts From The Same Donor. ... Cold Ischemia And Renal Graft Survival: A Paired Analysis Comparing First And Second Grafts From The... ... with special interest in cold ischemia times (CIT) as a risk factor for graft survival. METHODS: We retrospectively reviewed ...
more infohttp://medbrary.com.es/publication/p/cbbfc9cd509a6a1d/t/Cold-Ischemia-And-Renal-Graft-Survival%3A-A-Paired-Analysis-Comparing-First-And-Second-Grafts-From-The-Same-Donor.

HKU Scholars Hub: The impact of the establishment of a multiorgan transplantation program on cold ischemia time and delayed...HKU Scholars Hub: The impact of the establishment of a multiorgan transplantation program on cold ischemia time and delayed...

Article: The impact of the establishment of a multiorgan transplantation program on cold ischemia time and delayed graft ... The impact of the establishment of a multiorgan transplantation program on cold ischemia time and delayed graft function in ... The impact of the establishment of a multiorgan transplantation program on cold ischemia time and delayed graft function in ... cold+ischemia+time+and+delayed+graft+function+in+renal+transplantation. en_HK. ...
more infohttp://hub.hku.hk/handle/10722/77732

Tumor Pre-Analytics in Molecular Pathology: Impact on Protein Expression and Analysis | SpringerLinkTumor Pre-Analytics in Molecular Pathology: Impact on Protein Expression and Analysis | SpringerLink

Cold Ischemia. The common definition for cold ischemia in surgery is the time between the chilling of a tissue, organ, or body ... The effects of warm and cold ischemia can be classified into ischemia-induced metabolic responses including posttranslational ... Effects of cold ischemia on gene expression: a review and commentary. Biopreserv Biobank. 2016;14(6):548-58.CrossRefPubMed ... Cold ischemia triggers a cascade of noxious effects and responses to hypoxia and stress, all of which affect the quality of the ...
more infohttps://link.springer.com/article/10.1007%2Fs40139-018-0179-5

KAKEN - Research Projects | The effect of oxygenated warm buffer perfusion with PGE1 before cold preservation on hepatic warm...KAKEN - Research Projects | The effect of oxygenated warm buffer perfusion with PGE1 before cold preservation on hepatic warm...

The effect of oxygenated warm buffer perfusion with PGE1 before cold preservation on hepatic warm ischemia reperfusion injury. ... Journal Article] Perfusion Using Oxygenated Buffer Containing Prostaglandin E1 before Cold Preservation Prevents Warm Ischemia- ... Presentation] Perfusion by oxygenated buffer containing prostaglandin E1 prior to cold preservation prevents warm ischemia- ... Presentation] Perfusion by oxygenated buffer containing prostaglandin E1 prior to cold preservation prevents warm ischemia- ...
more infohttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21591626/

Most Popular Articles : Current Opinion in Organ TransplantationMost Popular Articles : Current Opinion in Organ Transplantation

The need to reduce cold ischemia time in kidney transplantation. van der Vliet, J. Adam; Warlé, Michiel C. ... Preservation solutions for static cold storage of abdominal allografts: which is best?. Parsons, Ronald F.; Guarrera, James V. ... Role of temperature in reconditioning and evaluation of cold preserved kidney and liver grafts. Minor, Thomas; von Horn, ... Donor risk factors, retrieval technique, preservation and ischemia/reperfusion injury in pancreas transplantation. Maglione, ...
more infohttps://journals.lww.com/co-transplantation/pages/viewallmostpopulararticles.aspx?WT.mc_id=HPxADx20100319xMP
  • In conclusion, PARP inhibition attenuates ATN and other IR-related renal lesions in mouse kidneys under prolonged cold storage in UW solution. (hindawi.com)
  • It was 74% for 6 hours of cold ischemia, 59% for 12 hours, 50% for 18 hours, 43% for 24 hours, and 0% for 48 hours of cold exposure. (cryonics.org)
  • PURPOSE The safe duration of ischemia during nephron sparing surgery remains controversial. (semanticscholar.org)
  • The coexistense of an abdominal aortic aneurysm with ectopic main renal vasculature complicates aortic surgery and mandates a focused imaging evaluation and a carefully planned operation to minimize renal ischemia. (biomedsearch.com)
  • These data suggest that ischemia primarily affects electron transport in the respiratory chain rather than the hydrogen shuttle and the energy coupling system. (springer.com)
  • I tried to use some compounds for some dehydration of rat brains to improve cold storage of the brains. (cryonics.org)
  • Scientists also did not repeat Dr. Suda's success for other organs those are more resistant to cold storage and freezing than the brain. (cryonics.org)