Cofilin 1: Cofilin 1 is a member of the cofilin family of proteins that is expressed in non-muscle CELLS. It has ACTIN depolymerization activity that is dependent on HYDROGEN-ION CONCENTRATION.Actin Depolymerizing Factors: A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. They remodel the actin CYTOSKELETON by severing ACTIN FILAMENTS and increasing the rate of monomer dissociation.Cofilin 2: A member of the cofilin family of proteins that is expressed in MUSCLE CELLS. It has ACTIN depolymerization activity that is dependent on HYDROGEN-ION CONCENTRATION.Lim Kinases: Serine protein kinases involved in the regulation of ACTIN polymerization and MICROTUBULE disassembly. Their activity is regulated by phosphorylation of a threonine residue within the activation loop by intracellular signaling kinases such as P21-ACTIVATED KINASES and by RHO KINASE.Destrin: A member of the actin depolymerizing factors. Its depolymerizing activity is independent of HYDROGEN-ION CONCENTRATION.Microfilament Proteins: Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.Actins: Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.Actin Cytoskeleton: Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.Pseudopodia: A dynamic actin-rich extension of the surface of an animal cell used for locomotion or prehension of food.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Profilins: A family of low molecular weight proteins that bind ACTIN and control actin polymerization. They are found in eukaryotes and are ubiquitously expressed.Gelsolin: A 90-kDa protein produced by macrophages that severs ACTIN filaments and forms a cap on the newly exposed filament end. Gelsolin is activated by CALCIUM ions and participates in the assembly and disassembly of actin, thereby increasing the motility of some CELLS.p21-Activated Kinases: A family of serine-threonine kinases that bind to and are activated by MONOMERIC GTP-BINDING PROTEINS such as RAC GTP-BINDING PROTEINS and CDC42 GTP-BINDING PROTEIN. They are intracellular signaling kinases that play a role the regulation of cytoskeletal organization.Cell Surface Extensions: Specialized structures of the cell that extend the cell membrane and project out from the cell surface.Actin-Related Protein 2-3 Complex: A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments.Phalloidine: Very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of liver damage.Cytoskeleton: The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.Ethenoadenosine Triphosphate: 1,N-6-Ethenoadenosine triphosphate. A fluorescent analog of adenosine triphosphate.rho-Associated Kinases: A group of intracellular-signaling serine threonine kinases that bind to RHO GTP-BINDING PROTEINS. They were originally found to mediate the effects of rhoA GTP-BINDING PROTEIN on the formation of STRESS FIBERS and FOCAL ADHESIONS. Rho-associated kinases have specificity for a variety of substrates including MYOSIN-LIGHT-CHAIN PHOSPHATASE and LIM KINASES.Actin-Related Protein 3: A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. It is expressed at higher levels than ARP2 PROTEIN and does not contain a PROFILIN binding domain.Cell Movement: The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.Thiazolidines: Reduced (protonated) form of THIAZOLES. They can be oxidized to THIAZOLIDINEDIONES.Biopolymers: Polymers synthesized by living organisms. They play a role in the formation of macromolecular structures and are synthesized via the covalent linkage of biological molecules, especially AMINO ACIDS; NUCLEOTIDES; and CARBOHYDRATES.Actin-Related Protein 2: A PROFILIN binding domain protein that is part of the Arp2-3 complex. It is related in sequence and structure to ACTIN and binds ATP.Phosphoprotein Phosphatases: A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)Nerve Tissue ProteinsPolymerization: Chemical reaction in which monomeric components are combined to form POLYMERS (e.g., POLYMETHYLMETHACRYLATE).CapZ Actin Capping Protein: An actin capping protein that binds to the barbed-ends of ACTIN filaments. It is a heterodimer consisting of an alpha and a beta subunit. It regulates actin assembly by stabilizing actin oligomers for elongation. In SKELETAL MUSCLE, CapZ is localized to the Z-disk.Protein Kinases: A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.rho GTP-Binding Proteins: A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47.Dendritic Spines: Spiny processes on DENDRITES, each of which receives excitatory input from one nerve ending (NERVE ENDINGS). They are commonly found on PURKINJE CELLS and PYRAMIDAL CELLS.Contractile Proteins: Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.RNA, Small Interfering: Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.Cytoskeletal Proteins: Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.Actin Capping Proteins: Actin capping proteins are cytoskeletal proteins that bind to the ends of ACTIN FILAMENTS to regulate actin polymerization.Bicyclo Compounds, Heterocyclic: A class of saturated compounds consisting of two rings only, having two or more atoms in common, containing at least one hetero atom, and that take the name of an open chain hydrocarbon containing the same total number of atoms. (From Riguady et al., Nomenclature of Organic Chemistry, 1979, p31)Phosphoric Monoester Hydrolases: A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.Stress Fibers: Bundles of actin filaments (ACTIN CYTOSKELETON) and myosin-II that span across the cell attaching to the cell membrane at FOCAL ADHESIONS and to the network of INTERMEDIATE FILAMENTS that surrounds the nucleus.Phosphatidylinositol 4,5-Diphosphate: A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)14-3-3 Proteins: A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Protein Footprinting: A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.Glia Maturation Factor: A factor identified in the brain that influences the growth and differentiation of NEURONS and NEUROGLIA. Glia maturation factor beta is the 17-kDa polypeptide product of the GMFB gene and is the principal component of GLIA MATURATION FACTOR.Binding Sites: The parts of a macromolecule that directly participate in its specific combination with another molecule.Growth Cones: Bulbous enlargement of the growing tip of nerve axons and dendrites. They are crucial to neuronal development because of their pathfinding ability and their role in synaptogenesis.Organelle Shape: The quality of surface form or outline of ORGANELLES.Protein Transport: The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.Cell Membrane Structures: Structures which are part of the CELL MEMBRANE or have cell membrane as a major part of their structure.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.

The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics. (1/145)

Actin-depolymerizing factor (ADF)/cofilins are essential regulators of actin filament turnover. Several ADF/cofilin isoforms are found in multicellular organisms, but their biological differences have remained unclear. Herein, we show that three ADF/cofilins exist in mouse and most likely in all other mammalian species. Northern blot and in situ hybridization analyses demonstrate that cofilin-1 is expressed in most cell types of embryos and adult mice. Cofilin-2 is expressed in muscle cells and ADF is restricted to epithelia and endothelia. Although the three mouse ADF/cofilins do not show actin isoform specificity, they all depolymerize platelet actin filaments more efficiently than muscle actin. Furthermore, these ADF/cofilins are biochemically different. The epithelial-specific ADF is the most efficient in turning over actin filaments and promotes a stronger pH-dependent actin filament disassembly than the two other isoforms. The muscle-specific cofilin-2 has a weaker actin filament depolymerization activity and displays a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1 and ADF. In steady-state assays, cofilin-2 also promotes filament assembly rather than disassembly. Taken together, these data suggest that the three biochemically distinct mammalian ADF/cofilin isoforms evolved to fulfill specific requirements for actin filament dynamics in different cell types.  (+info)

The human D2 dopamine receptor synergizes with the A2A adenosine receptor to stimulate adenylyl cyclase in PC12 cells. (2/145)

The adenosine A(2A) receptor and the dopamine D(2) receptor are prototypically coupled to G(s) and G(i)/G(o), respectively. In striatal intermediate spiny neurons, these receptors are colocalized in dendritic spines and act as mutual antagonists. This antagonism has been proposed to occur at the level of the receptors or of receptor-G protein coupling. We tested this model in PC12 cells which endogenously express A(2A) receptors. The human D(2) receptor was introduced into PC12 cells by stable transfection. A(2A)-agonist-mediated inhibition of D(2) agonist binding was absent in PC12 cell membranes but present in HEK293 cells transfected as a control. However, in the resulting PC12 cell lines, the action of the D(2) agonist quinpirole depended on the expression level of the D(2) receptor: at low and high receptor levels, the A(2A)-agonist-induced elevation of cAMP was enhanced and inhibited, respectively. Forskolin-stimulated cAMP formation was invariably inhibited by quinpirole. The effects of quinpirole were abolished by pretreatment with pertussis toxin. A(2A)-receptor-mediated cAMP formation was inhibited by other G(i)/G(o)-coupled receptors that were either endogenously present (P(2y12)-like receptor for ADP) or stably expressed after transfection (A(1) adenosine, metabotropic glutamate receptor-7A). Similarly, voltage activated Ca(2+) channels were inhibited by the endogenous P(2Y) receptor and by the heterologously expressed A(1) receptor but not by the D(2) receptor. These data indicate functional segregation of signaling components. Our observations are thus compatible with the proposed model that D(2) and A(2A) receptors are closely associated, but they highlight the fact that this interaction can also support synergism.  (+info)

Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. (3/145)

Actin-depolymerizing factor (ADF)/cofilins are small actin-binding proteins found in all eukaryotes. In vitro, ADF/cofilins promote actin dynamics by depolymerizing and severing actin filaments. However, whether ADF/cofilins contribute to actin dynamics in cells by disassembling "old" actin filaments or by promoting actin filament assembly through their severing activity is a matter of controversy. Analysis of mammalian ADF/cofilins is further complicated by the presence of multiple isoforms, which may contribute to actin dynamics by different mechanisms. We show that two isoforms, ADF and cofilin-1, are expressed in mouse NIH 3T3, B16F1, and Neuro 2A cells. Depleting cofilin-1 and/or ADF by siRNA leads to an accumulation of F-actin and to an increase in cell size. Cofilin-1 and ADF seem to play overlapping roles in cells, because the knockdown phenotype of either protein could be rescued by overexpression of the other one. Cofilin-1 and ADF knockdown cells also had defects in cell motility and cytokinesis, and these defects were most pronounced when both ADF and cofilin-1 were depleted. Fluorescence recovery after photobleaching analysis and studies with an actin monomer-sequestering drug, latrunculin-A, demonstrated that these phenotypes arose from diminished actin filament depolymerization rates. These data suggest that mammalian ADF and cofilin-1 promote cytoskeletal dynamics by depolymerizing actin filaments and that this activity is critical for several processes such as cytokinesis and cell motility.  (+info)

Maternal diet programs embryonic kidney gene expression. (4/145)

Human epidemiological data associating birth weight with adult disease suggest that organogenesis is "programmed" by maternal diet. In rats, protein restriction in pregnancy produces offspring with fewer renal glomeruli and higher systemic blood pressures than controls. We tested the hypothesis that maternal diet alters gene expression in the metanephros, the precursor of the definitive mammalian kidney. We demonstrated that maternal low-protein diet initiated when pregnancy starts and maintained to embryonic day 13, when the metanephros consists of mesenchyme surrounding a once-branched ureteric bud, is sufficient to significantly reduce glomerular numbers in offspring by about 20%. As assessed by representational difference analyses and real-time quantitative polymerase chain reactions, low-protein diet modulated gene expression in embryonic day 13 metanephroi. In particular, levels of prox-1, the ortholog of Drosophila transcription factor prospero, and cofilin-1, a regulator of the actin cytoskeleton, were reduced. During normal metanephrogenesis, prox-1 protein was first detected in mesenchymal cells around the ureteric tree and thereafter in nascent nephron epithelia, whereas cofilin-1 immunolocalized to bud derivatives and condensing mesenchyme. Previously, we reported that low-protein diets increased mesenchymal apoptosis cells when metanephrogenesis began and thereafter reduced numbers of precursor cells. Collectively, these studies prove that the maternal diet programs the embryonic kidney, altering cell turnover and gene expression at a time when nephrons and glomeruli have yet to form. The human implication is that the maternal diet ingested between conception and 5- 6-wk gestation contributes to the variation in glomerular numbers that are known to occur between healthy and hypertensive populations.  (+info)

Micropuncture gene delivery and intravital two-photon visualization of protein expression in rat kidney. (5/145)

Understanding molecular mechanisms of pathophysiology and disease processes requires the development of new methods for studying proteins in animal tissues and organs. Here, we describe a method for adenoviral-mediated gene transfer into tubule or endothelial cells of the rat kidney. The left kidney of an anesthetized rat was exposed and the lumens of superficial proximal tubules or vascular welling points were microinfused, usually for 20 min. The microinfusion solution contained adenovirus with a cDNA construct of either 1) Xenopus laevis actin depolymerizing factor/cofilin [XAC; wt-green fluorescent protein (GFP)], 2) actin-GFP, or 3) GFP. Sudan black-stained castor oil, injected into nearby tubules, allowed us to localize the microinfused structures for subsequent visualization. Two days later, the rat was anesthetized and the kidneys were fixed for tissue imaging or the left kidney was observed in vivo using two-photon microscopy. Expression of GFP and GFP-chimeric proteins was clearly seen in epithelial cells of the injected proximal tubules and the expressed proteins were localized similarly to their endogenously expressed counterparts. Only a minority of the cells in the virally exposed regions, however, expressed these proteins. Endothelial cells also expressed XAC-GFP after injection of the virus cDNA construct into vascular welling points. An advantage of the proximal tubule and vascular micropuncture approaches is that only minute amounts of virus are required to achieve protein expression in vivo. This micropuncture approach to gene transfer of the virus cDNA construct and intravital two-photon microscopy should be applicable to study of the behavior of any fluorescently tagged protein in the kidney and shows promise in studying renal physiology and pathophysiology.  (+info)

Ligation of cell surface-associated glucose-regulated protein 78 by receptor-recognized forms of alpha 2-macroglobulin: activation of p21-activated protein kinase-2-dependent signaling in murine peritoneal macrophages. (6/145)

Previous studies of the plasma proteinase inhibitor alpha2-macroglobulin (alpha2M) demonstrated that alpha2M-proteinase complexes (alpha2M*) modulate immune responses and promotes macrophage locomotion and chemotaxis. Alpha2M* binds to cell surface-associated glucose-regulated protein 78 (GRP78), which activates downstream signaling events. The role of p21-activated protein kinase-1 and -2 (PAK-1 and -2) in promoting cellular motility is well documented. In the current study, we examined the ability of alpha2M* to activate PAK-1 and PAK-2. Upon macrophage stimulation with alpha2M*, PAK-2 is autophosphorylated, resulting in increased kinase activity; however, PAK-1 is negligibly affected. Alpha2M*-stimulated macrophages showed a marked elevation in the levels of Rac x GTP. Receptor tyrosine phosphorylation upon binding of alpha2M* to GRP78, recruits PAK-2 to the plasma membrane via the adaptor protein NCK. Consistent with this hypothesis, silencing of GRP78 gene expression greatly attenuated the levels of membrane-associated PAK-2 and NCK. PAK-2 activity was markedly decreased by inhibition of tyrosine kinases and PI3K before alpha2M* stimulation. We further demonstrate that phosphorylation of Lin-11, Isl-1, Mec-3 (LIM) kinase and cofilin is promoted by treating macrophages with alpha2M*. Thus, alpha2M* regulates activation of the PAK-2-dependent motility mechanism in these cells.  (+info)

Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C. (7/145)

BACKGROUND: Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS: A method for DNA splicing and mutagenesis without restriction enzymes is described. The method is based on mild template-dependent polymerization arrest with two molecules of cytosine arabinose (Ara-C) incorporated into PCR primers. Two rounds of PCR are employed: the first PCR produces 5' overhangs that are utilized for DNA splicing. The second PCR is based on polymerization running through the Ara-C molecules to produce the desired final product. To illustrate application of the run through stop mutagenesis and DNA splicing technique, we have carried out splicing of two segments of the human cofilin 1 gene and introduced a mutational deletion into the product. CONCLUSION: We have demonstrated the utility of a new PCR-based method for carrying out DNA splicing and mutagenesis by incorporating Ara-C into the PCR primers.  (+info)

Stimulation of actin polymerization by filament severing. (8/145)

The extent and dynamics of actin polymerization in solution are calculated as functions of the filament severing rate, using a simple model of in vitro polymerization. The model is solved by both analytic theory and stochastic-growth simulation. The results show that severing essentially always enhances actin polymerization by freeing up barbed ends, if barbed-end cappers are present. Severing has much weaker effects if only pointed-end cappers are present. In the early stages of polymerization, the polymerized-actin concentration grows exponentially as a function of time. The exponential growth rate is given in terms of the severing rate, and the latter is given in terms of the maximum slope in a polymerization time course. Severing and branching are found to act synergistically.  (+info)

During early metastatic events in cancer, reorganization of the actin cytoskeleton must occur to allow for cell invasion and migration. A key enzyme initiating actin remodeling at the leading edge is Cofilin, a small actin binding protein of the ADF/cofilin family with actin severing activities. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation events that are mediated by LIM Kinase (LIMK) and the phosphatase Slingshot (SSH), respectively. Protein kinase D (PKD) is a serine/threonine kinase that has previously been shown to regulate actin driven directed cell migration through phosphorylation and thus inactivation of Slingshot. Here we show that PKD can also regulate cofilin activity via activation of LIMK. We further show that PKD-induced regulation of LIMK and the resulting diminished cofilin activity translates to altered cell motility. Our data suggest that PKD inactivates Cofilin through modulation of both of its regulatory pathways. Further elucidation of the ...
Cofilin 1 (non-muscle; n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. One group reports that reelin signaling leads to serine3-phosphorylation of cofilin-1, and this interaction may play a role in the reelin-related regulation of neuronal migration. Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000056201 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". "Entrez Gene: CFL1 cofilin 1 (non-muscle)". Chai X, Förster E, Zhao S, Bock HH, Frotscher M (January 2009). "Reelin stabilizes the actin cytoskeleton of neuronal processes by inducing n-cofilin ...
The actin depolymerizing factor (ADF)/cofilin protein family is essential for actin dynamics, cell division, chemotaxis and tumor metastasis. Cofilin-1 (CFL-1) is a primary non-muscle isoform of the ADF/cofilin protein family accelerating the actin filamental turnover in vitro and in vivo. In response to environmental stimulation, CFL-1 enters the nucleus to regulate the actin dynamics. Although the purpose of this cytoplasm-nucleus transition remains unclear, it is speculated that the interaction between CFL-1 and DNA may influence various biological responses, including DNA damage repair. In this review, we will discuss the possible involvement of CFL-1 in DNA damage responses (DDR) induced by ionizing radiation (IR), and the implications for cancer radiotherapy.
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A) PirB interacts with cofilin in vivo in PirB+/− Tg mice (P30, forebrain), assessed by immunoprecipitation for PirB. Other known PirB-proximal signaling and interactions such as tyrosine phosphorylation of PirB and SHP-2 recruitment to PirB are not altered in PirB+/− relative to PirB+/− Tg mice. Representative data are shown (n , 2 ...
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Cofilin 2 antibody (cofilin 2 (muscle)) for WB. Anti-Cofilin 2 pAb (GTX14133) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Mouse Monoclonal Anti-Cofilin Antibody (1A1) [DyLight 755]. Validated: WB, ELISA, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Mouse. 100% Guaranteed.
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
... MAPK 経路 LIMK, subsequent phosphorylation of cofilin, and migration of major human T cells in the three dimen
Having an existing gmfgen file and a generated diagram plug-in, but trying to generate the same diagram plug-in results in different java classes structure ...
Having an existing gmfgen file and a generated diagram plug-in, but trying to generate the same diagram plug-in results in different java classes structure ...
The IS is a polarized cell structure similar to the leading edge of a migrating cell or the yeast bud site. Establishing functional patterns of membrane protein organization and vesicular traffic at these sites requires coordinated reorganization of the actin and microtubule cytoskeletons. Rap1 is an evolutionarily conserved regulator of cell polarity that drives actin reorganization at the B cell IS (Lin et al., 2008). We have now shown that Rap1, and its downstream target, the actin-severing protein cofilin, are essential for BCR-induced MTOC polarization towards the IS. Whether Rap1 controls MTOC reorientation in other immune cells is not known. The BCR and TCR both activate Rap1 via phospholipase Cγ-dependent production of diacylglycerol (Katagiri et al., 2004; McLeod et al., 1998), which recruits the Rap1 exchange factor RasGRP2 (also known as CalDAG-GEFI) (Kawasaki et al., 1998) to the plasma membrane. The scaffolding protein ADAP (also known as FYB), which promotes the accumulation or ...
To make Angry Birds a real sport, Mbed made a USB slingshot to play Angry Birds. The slingshot emulates a USB mouse, so it really is a plug n play. It translates the physical use of the slingshot in to appropriate mouse controls. The way the controller works is reasonably simple: a stretch sensor translates the drawing of the slingshot to a mouse movement and an accelerometer determines the angle. Check out the video of it in action. ...
SLINGSHOT™: Athletic Performance and Endurance Maximizer SLINGSHOT™ YOUR PERFORMANCE TO THE NEXT LEVEL! SLINGSHOT™ athletic
Alzheimers disease (AD) is a neurodegenerative disorder characterized by two major pathological hallmarks, amyloid plaques and neurofibrillary tangles. The accumulation of amyloid-β protein (Aβ) is an early event associated with synaptic and mitochondrial damage in AD. Therefore, molecular pathways underlying the neurotoxicity and generation of Aβ represent promising therapeutic targets for AD. Recent studies have shown that actin severing protein, Cofilin plays an important role in synaptic remodeling, mitochondrial dysfunction, and AD pathogenesis. However, whether Cofilin is an essential component of AD pathogenesis and how Aβ induced neurotoxicity impinges its signals to Cofilin are unclear. In my dissertation studies, we found Aβ oligomers bind with intermediate activation conformers of β1-integrin to induce the loss of surface β1-integrin and activation of Cofilin via Slingshot homology-1 (SSH1) activation. Specifically, conditional loss of β1-integrin prevented Aβ induced Cofilin
Cofilin belongs to the actin-binding ADF protein family. It contains one ADF-H domain. Cofilin controls reversible actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin at a 1:1 ratio. It is the major component of both intranuclear and cytop...
Real‐time imaging. Cells were seeded in a Lab‐Tek Chambered #1.0 Borosilicate Coverglass System (Nalgene Nunc International, Rochester, NY, USA) and were mounted on a Zeiss Axiovert 200M microscope for live‐cell imaging (5% CO2; 37°C) for 2-14 h. Phase‐contrast images were captured every 2 min using a Photometics Coolsnap CCD camera (Scientific, Tuscon, AZ, USA). Images were processed using the Metamorph software (Universal imaging, Downington, PA, USA). Several protrusions was scored by off‐line analysis of the generated videos and plotted as means±s.e.m. For real‐time fluorescence imaging, cells were placed on a Leica SP5 inverted microscope equipped with a × 63 1.3NA glycerol objective. Cells were maintained in Leibovitz‐15‐buffered medium (Invitrogen, Breda, The Netherlands) at 37°C in a climate chamber.. Invasion assay. For in vitro invasion assays, 24‐well BioCoat matrigel invasion chambers (#354480, BD Biosciences, Alphen aan den Rijn, The Netherlands) were used ...
PAK4_HUMAN] Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of ...
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Actin-severing proteins ADF/cofilin are required for the sorting of secretory cargo at the trans-Golgi network (TGN) in mammalian cells. How do these cytoplasmic proteins interact with the cargoes in the lumen of the TGN ...
Reporting on the project, Dr Goldsbury wrote, "In this project supported by the SZCUF, we have demonstrated that reduced energy metabolism by inhibition of mitochondrial function in cultured neurons gives rise to the redistribution of tau and cofilin into rod-like structures that appear to mimic those in the AD brain. Further, we demonstrated that the density of cofilin rods in human AD brains is significantly higher than in normal aged human brain. However, we also showed that some aged normal brains contained low levels of cofilin rods, although these brains were devoid of abnormal tau. This suggests that cofilin rod generation may be an early event in the neurodegenerative cascade representing a possible early-stage mechanism in the progression to AD dementia and therefore an ideal future target for therapeutic intervention that might be useful in treatment of neurological diseases. Unexpectedly, however, despite an extensive study, we were not able to show any substantial microscopic overlap ...
One candidate early on was a protein called slingshot, which seemed to have the correct phosphatase activity. However, slingshot turned out not to be found in all organisms, nor ubiquitously distributed throughout the body, a requirement for the mystery phosphatase, as it would be needed wherever actin dynamics is taking place. Additionally, slingshot did not appear to be capable of regulating all cofilin-dependent cell processes, so clearly a third man still existed.. Now in an article recently published in Nature Cell Biology, Bokoch and his colleagues Antje Gohla and Jörg Birkenfeld argue that the third man phosphatase is actually chronophin. Taking the increasingly overlooked approach of function-based biochemical isolation, which required Gohla to spend significant time in the cold room, the scientists purified a phosphatase activity which dephosphorylated cofilin. This activity was then identified by mass spectrometry as chronophin, a member of a unique superfamily of proteins called ...
The SR-7 Wrist-Rocket® is a durable entry level slingshot. Molded handle Self centering pouch Tubular bands with protective prong caps to extend band life Made …
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This Forum contains Member Profiles. These members have achieved Hall of Fan Status (5000 posts) and as such have demonstrated their commitment to the CFL and 13thman.com. Hall of Fan Members will of course receive respect and admiration from your peers and have the opportunity to place your profile here ...
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I would have gone, but I couldnt find a slingshot big enough. Oh, and I didnt realize there was a Cow Con III. But if it wasnt for those two things, I might have gone ...
The GAP first draft also has added the elements of NCDs Global Monitoring Framework (including twenty-five indicators) and Voluntary Global Targets (nine targets) finalised in the WHO meeting of member states late last year (5-7 November 2012). However the stakeholders want the 25 indicators of Global Monitoring Framework (GMF) and 9 voluntary global targets should be integrated in GAP alongwith evidence based interventions. Let us examine the GMF and GAP in some detail: ...
For the SSH-1 protocol, see Secure Shell#Version 1.x Protein phosphatase Slingshot homolog 1 is an enzyme that in humans is encoded by the SSH1 gene. The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002).[supplied by OMIM] GRCh38: Ensembl release 89: ENSG00000084112 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000042121 - Ensembl, May 2017 "Human PubMed Reference:". "Mouse PubMed Reference:". Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O (Apr 2000). "Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (1): 65-73. doi:10.1093/dnares/7.1.65. PMID 10718198. Niwa ...
Cofilin phospho-regulation is important for actin filament turnover and is implicated in cancer. Phosphorylation of cofilin is mediated by LIM kinases (LIMKs) and dephosphorylation by Slingshot phosphatases (SSH). LIMKs and SSH promote cancer cell invasion and metastasis and represent novel anti-cancer targets. However, little is known regarding LIMK/cofilin and SSH in human colorectal cancer (CRC). In this study, we aimed to address their expression and significance in human CRC. We evaluated expression of non-phosphorylated (active) and phosphorylated cofilin, LIMK1, LIMK2, and SSH1 by immunohistochemistry in 143 human CRC samples in relation to clinicopathologic parameters, response of metastatic disease to chemotherapy, and epithelial-mesenchymal transition (EMT) markers β-catenin, E-cadherin, and ZEB ...
To confirm the morphological effects of 5-HT6Rs transfected in neuronal cells, we finally examined whether the activation of neuronal 5-HT6Rs produces effects on RhoA activities. To perform RhoA activity assay as shown in Fig. 2, we carried immunoblot analysis using cultured cortical neurons for sufficient amount of sample proteins. Here, we showed that 5-HT6R activation by ST1936 significantly increased RhoA-GTP level, which was significantly blocked by the pretreatment with SB258585 in cultured neurons (Figs. 4A and 4B). As downstream targets of Rho proteins, RhoA proteins can also affect actin polymerization by regulating cofilin via Rho-ROCK-LIMK-cofilin pathway (Lin et al., 2003). Therefore, we further executed whether the activation of 5-HT6Rs modulates cofilin activities as a downstream target of RhoA. As shown in Figs. 4C and 4D, we observed that treatment with ST1936 significantly increased phosphorylation of cofilin, which was also significantly blocked by SB258585 in cultured neurons. ...
To confirm the morphological effects of 5-HT6Rs transfected in neuronal cells, we finally examined whether the activation of neuronal 5-HT6Rs produces effects on RhoA activities. To perform RhoA activity assay as shown in Fig. 2, we carried immunoblot analysis using cultured cortical neurons for sufficient amount of sample proteins. Here, we showed that 5-HT6R activation by ST1936 significantly increased RhoA-GTP level, which was significantly blocked by the pretreatment with SB258585 in cultured neurons (Figs. 4A and 4B). As downstream targets of Rho proteins, RhoA proteins can also affect actin polymerization by regulating cofilin via Rho-ROCK-LIMK-cofilin pathway (Lin et al., 2003). Therefore, we further executed whether the activation of 5-HT6Rs modulates cofilin activities as a downstream target of RhoA. As shown in Figs. 4C and 4D, we observed that treatment with ST1936 significantly increased phosphorylation of cofilin, which was also significantly blocked by SB258585 in cultured neurons. ...
Abstract: A key aspect of cell and tissue morphogenesis is regulation of the actin cytoskeleton. The actin cytoskeleton is a fundamental cellular scaffold which...
Fads remind me of an iPad game called Slingshot Racing. What you do is, there are these tiny cars that run around a tiny oval racetrack, and you have to click on the screen (tech faux pas! tap on the screen) to tether the cars to tiny towers that slingshot them around the corners (ends? short sides?) of the oval. It sounds easy but in fact its devastatingly hard to tap and release at exactly the right times to start the tether and stop it without smashing your tiny car into a tiny wall. Thats kind of like catching and riding a fad. You have to discover that the potential for a fad exists, and not just any fad; one you can make money on, say by selling rocks or playing the saxophone in a particular way. You have to tether the fad to you at just the right moment, then hold onto it just up until the moment when its about to fade, at which point you release it before you start looking stupid. Few succeed at this game. Some tap too early and suffer ridicule, that is, until after theyre dead and ...
Ladies, and gentleman, I would like to present the winner of the Grey Cup, this year... Buffalo! Although the idea of a CFL commissioner making such a statement in the future seems far fetched, ...
J:194042 Mahaffey JP, Grego-Bessa J, Liem KF Jr, Anderson KV, Cofilin and Vangl2 cooperate in the initiation of planar cell polarity in the mouse embryo. Development. 2013 Mar;140(6):1262-71 ...
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2019- 2020學年(上學期)????? (2019.10.14-2019.10.18)課題組名稱(聯系人)時? 間地? 點主講人題?? 目理論物理研究所 (李世淵)星期三15:30知新樓C913??交叉科學研究所 (劉向東)星期二下午離子束報告廳????????原子分子物理 (許國富)星期三下午知新樓C1011??????????凝聚態理論研究室 (崔彬)星期四14:30知新樓C1113季藝聞基于SSH模型對A-D-A類分子建模????電介質物理研究室 (趙明磊)星期五14:30知新樓C1113??...
Having identified changes in the extent of cofilin phosphorylation in cells at steady state, we next examined the dynamics of cofilin phosphorylation in cells undergoing acute changes in actin cytoskeletal organisation. This required the identification of a stimulus able to induce dynamic actin remodelling. In testing a number of growth factors in S2R+ cells [10% foetal calf serum (FCS), 2-day-old conditioned medium, bovine insulin, human EGF, murine vascular endothelial growth factor (VEGF) and human platelet-derived growth factor (PDGF)], only insulin was found to activate signalling, as monitored by immunoblotting with specific antibodies against P-Akt, S6K phosphorylated at Thr398 (P-S6K) and ERK phosphorylated at Thr198 and Tyr200 (PP-ERK) (supplementary material Fig. S3A) and Lizcano et al. (Lizcano et al., 2003). Insulin also induced an increase in the level of lamellipodial F-actin in these cells within 5 minutes of treatment (Fig. 2A). Elevated levels of filamentous actin remained at ...
The surge of actin growth toward the cell periphery is stopped abruptly by capping protein (CP). In vitro, this protein sticks like glue, but in vivo it comes off the ends of those filaments rapidly, say Miyoshi et al. (page 947). Cycles of severing and annealing may thus be more important than expected in creating a dynamic actin array.. The researchers had previously tracked the lifetimes of actin filaments in the lamellipodia of spreading Xenopus fibroblasts. They sought explanations for the range of lifetimes by looking at actin regulatory proteins such as CP. Surprisingly, the binding half life of CP in vivo was barely more than a second, compared with almost half an hour in vitro. The uncapped filaments appeared to resume their growth.. Although actin turnover is ∼20-fold slower than CP turnover, a further reduction in actin turnover by adding a cofilin inhibitor significantly reduced CP turnover. The authors suggest that cofilin-mediated filament severing may cause the dissociation of ...
In the fall of 2017, NPR Music and VuHaus' public radio network kicked off Slingshot, a collaboration among taste-making music radio stations to
As a freelance broadcast technician, some days I have to get up at 3AM to run camera, lights, and sound for corporate news shows like Good Morning America, The Early Show, and Meet the Press. The combination of sleep deprivation and old white guys yakking routinely tests my ability to stay awake and attentive. In order to pass the time, I sometimes try to focus less on the news, and more on the construction of the news and its discourses, lending equal attention to form and content. The producers know that people will be watching through pre-coffee morning haze -these shows are called "Breakfast Television," after all. So, what is being communicated underneath and between the script, so subtle it wont distract from your toast, but so prevalent it structures the very way watch you watch? (What lends the anchor their authority as a speaker? Whos wearing makeup, and how much? Whos speaking on behalf of whom? Whats being sold?) These wonderings give me something to think about as I push the ...
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CALGARY - Canada's Jesse Lumsden has been training for seven hours a day in preparation for the upcoming bobsled season. The former CFL star will be watching as his former team - the Hamilton Tiger-Cats - takes on Saskatchewan in the Grey Cup. Lumsden plans to get together with Johnny Quinn - a
Actin-depolymerizing factor (ADF)/cofilins contribute to cytoskeletal dynamics by promoting rapid actin filament disassembly. In the classical view, ADF/cofilin sever filaments, and capping proteins block filament barbed ends whereas pointed ends depolymerize, at a rate that is still debated. Here, by monitoring the activity of the three mammalian ADF/cofilin isoforms on individual skeletal muscle and cytoplasmic actin filaments, we directly quantify the reactions underpinning filament severing and depolymerization from both ends. We find that, in the absence of monomeric actin, soluble ADF/cofilin can associate with bare filament barbed ends to accelerate their depolymerization. Compared to bare filaments, ADF/cofilin-saturated filaments depolymerize faster from their pointed ends and slower from their barbed ends, resulting in similar depolymerization rates at both ends. This effect is isoform specific because depolymerization is faster for ADF- than for cofilin-saturated filaments. We also ...
A Chilean model has released footage of the moment she suffered neck injuries after being accidentally shot out of a giant slingshot while filming a Nissan advert.. Maura Bellagamba claims a film agency shot her out of a slingshot without her consent, causing her whiplash. According to BioBioChile news, the incident occurred during the filming of a Nissan car commercial.. Footage of the bizarre accident has now been released publically and shared online by Bellagamba, who says she was lucky to escape more serious injury in the "terrifying" incident.. "Can you believe that I was the test doll myself," she told followers on Instagram. "We were supposed to do a little test but they threw me into the shot without my consent." The film company responsible for the Nissan advert, named as Acción Film, said Bellagamba was fired from the slingshot by accident.. "In no case was the objective to launch the professional, the company said in a statement to local media. Subsequently, all medical expenses ...
The neurotrophin receptor TrkA binds to nerve growth factor (NGF) and neurotrophin 3 (NT3) to activate extension of axons. However, only NGF triggers the formation of TrkA-containing signaling endosomes that are transported in a retrograde manner to the soma to promote neuronal survival. Harrington et al. found that stabilization of the actin cytoskeleton in mouse sympathetic neurons by jasplakinolide treatment prevented retrograde transport of TrkA endosomes, suggesting a requirement for actin depolymerization in this process. Higher amounts of the actin filament-severing protein cofilin associated with TrkA isolated from neurons treated with NGF compared with that from neurons treated with NT3. In addition, cofilin colocalized to a greater extent with TrkA endosomes in NGF-treated neurons than in those treated with NT3. Retrograde transport of TrkA endosomes was impaired in NGF-treated neurons expressing an shRNA directed against cofilin, an effect that was largely rescued by application of ...
And then there are fluids whose viscosity decreases as you squeeze them. These are the shear thinning fluids. This is like ketchup, that flows when you squeeze or shake the bottle, but wont flow off your burger. Paints work on the same principle. They will flow across the canvas when applied with the force of a brush, but wont drip when left alone.. And biofilms fall into this latter class of fluids. In the case of our bacteria, the researchers estimate that force of the slingshot is enough to lower the viscosity of the surrounding goo by three-fold.. By launching themselves forward, the bacteria are taking advantage of this quirk of physics to effectively slice through the slime. This is in contrast to the strategy adopted by the stomach bacteria Helicobacter pylori, that solves the problem using chemical engineering. H. pylori lives in the mucus lining of our stomachs, an alarmingly inhospitable environment for a life form. To help it move, it releases a chemical that thins out the ...
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Centrosome amplification (CA) is a hallmark of virtually all types of cancers including solid tumors and hematological malignancies. Cancer cells with extra centrosomes use centrosome clustering (CC) to allow for successful division. Because normal cells do not rely on this mechanism, CC is regarded as a promising target to selectively eradicate cells harboring supernumerary centrosomes. To identify novel inhibitors of CC, we developed a cell-based high-throughput screen that reports differential drug cytotoxicity for isogenic cell populations with different centrosome contents. We identified CP-673451 and crenolanib, two chemically related compounds originally developed for inhibition of PDGFR-β, as robust inhibitors of CC with selective cytotoxicity for cells with extra centrosomes. We demonstrate that these compounds induce mitotic spindle multipolarity by activation of the actin severing protein cofilin, leading to destabilization of the cortical actin network, and provide evidence that ...
The CFL fined the Saskatchewan Roughriders $31,500 for two bylaw violations and head coach/GM Chris Jones $5,000 for meeting with the publicist for former NFL quarterback Johnny Manziel. The Roughriders have also been fined for violating multiple CFL By-Laws after an extensive auditing process.
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1HQZ: Phased translation function revisited: structure solution of the cofilin-homology domain from yeast actin-binding protein 1 using six-dimensional searches.
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Update: Ive been playing with the timestep-dependence of this. In the pic below, the black line (solid) is run "a" (std) minus run "b" (small pert); black dashed is a-c, where "c" is a different small pert. Blue is the same but for with all three runs done at 1/2 the timestep. Red also, but for 1/4 the timestep. The std timestep is half hour. There are plenty of caveats here: firstly, that this is really only playing. Secondly, that all I did was change the value marked timestep (actually, the value marked "steps per period" from 48 to 96 to 192) without checking that anything was going hideously wrong elsewhere. Thirdly, that if you compare this to the previous plot youll notice that its spikier: because its 6-h data (instantaneous) not timestep data. Fourthly, that even if nothing is going hideously wrong, changing the timestep does give a different model (is the climatology the same? I dont know. Maybe). For example, the atmos is fourier-filtered at the poles for CFL reasons. A smaller ...
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TY - JOUR. T1 - HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1. AU - Quintela-Fandino, Miguel. AU - Arpaia, Enrico. AU - Brenner, Dirk. AU - Goh, Theo. AU - Yeung, Faith Au. AU - Blaser, Heiko. AU - Alexandrova, Roumiana. AU - Lind, Evan. AU - Tusche, Mike W.. AU - Wakeham, Andrew. AU - Ohashi, Pamela S.. AU - Mak, Tak W.. PY - 2010/2/9. Y1 - 2010/2/9. N2 - Metastasis leads to the death of most cancer patients, and basal breast cancer is the most aggressive breast tumor type. Metastasis involves a complex cell migration process dependent on cytoskeletal remodeling such that targeting such remodeling in tumor cells could be clinically beneficial. Here we show that Hormonally Upregulated Neu-associated Kinase (HUNK) is dramatically down-regulated in tumor samples and cell lines derived from basal breast cancers. Reconstitution of HUNK expression in basal breast cancer cell lines blocked actin polymerization and reduced cell ...
Differentiation of erythroblasts to mature red blood cells involves dynamic changes of the membrane and cytoskeleton networks that are not fully characterized. Using a mouse fetal liver erythroblast culture system and a targeted shRNA functional screening strategy, we identified a critical role of pleckstrin-2 in actin dynamics and protection of early stage terminal erythroblasts from oxidative damage. Knockdown of pleckstrin-2 in the early stage of terminal erythropoiesis disrupted the actin cytoskeleton and led to differentiation inhibition and apoptosis. This pro-survival and differentiation function of pleckstrin-2 was mediated through its interaction with cofilin, by preventing cofilin mitochondrial entry when the intracellular level of reactive oxygen species was higher in the early stage of terminal erythropoiesis. Treatment of the cells with a scavenger of reactive oxygen species rescued cofilin mitochondrial entry and differentiation inhibition induced by pleckstrin-2 knockdown. In ...
Former CFL player hopes for quick trial on charges. REGINA - A former CFL Saskatchewan Roughrider player is looking to deal with a domestic violence charge quickly.. On April 18, Justin Cox was charged with assault causing bodily harm after police were called to a Regina home.. Police said a 23-year-old woman was found with injuries consistent with a physical attack.. Cox pleaded not guilty Thursday and his trial was set for May 29.. Cox wasnt in court, but his lawyer, who made the plea on Coxs behalf, says they want to deal with the matter quickly as he is currently "without employment.". The Riders released Cox when the assault charge became public and the CFL also said he would no longer be allowed to play with any team in the league.. ...
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The Ghost Whisper is the newest edition to Slingshots rapidly expanding foil lineup. This sleek carbon foil is fine-tuned for all-conditions riding and aggressive freeride performance. As a testament to the commitment Slingshot has to developing the most comprehensive foil lineup in kiteboarding, the Ghost Whisper features a modular design and sturdy aluminum fuselage that allows riders to change wingsets to achieve dramatically different performance characteristics. Buying into the Ghost Whisper foil is an investment in an evolving program, not just a single product. The Ghost Whisper comes stock with a performance freeride setup designed for aggressive but all-around handling. A performance speedride wingset is currently available as an upgrade and is a high-aspect, high-efficiency setup tuned for more advanced riding at higher speeds. Additional wingsets are currently in development and will be released as they are completed ...
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I love bikes and I want everyone to experience the joy, freedom as well as ecological and health benefits of bicycles. Over the years, Ive realized that the standard bicycle designs arent comfortable for me - they make biking harder than it has to be and sometimes theyve caused me pain. So Ive gradually customized, rebuilt and re-designed my bike to overcome these problems. Through this 15 year process, Ive finally come up with what (at least for me) is the "perfect bicycle." It isnt an expensive bike - you can do this stuff with cheap or old bikes. Last summer I biked 4,000 miles across the USA on this bike - I was comfortable the whole way and finished the trip fast, in only 50 days. Of course, everyone is different so all of these suggestions might not work for you. But if youre riding a bike that you havent customized, if youre experiencing pain while biking, or if biking seems hard to you, you may want to try some of these suggestions and see if they help. 1. Raise your handle ...
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I suppose I should say something about the CFL East all-star selections. Nine Alouettes being selected obviously makes sense. Seven Argos is a bit dubious, but Toronto was strong on defence and special teams and sucked on offense, so some of the defensive and special teams players making does make a certain amount of sense. Strange that the Ticats only had four players, Bruce, Stala, Hage and Knowlton. Especially only one defensive player making the squad, considering the Cats ended up with a decent defence by the end of the season ...
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With a $0.99 game, players shouldnt expect much. Perhaps something interesting, or an addicting formula that has you coming back for more, but always something simple. I mean, who can afford to make a complex game for just a buck?. Angry Birds does all three, and marvelously. From developer ClickGamer, Angry Birds tells the simple story of a rivalry between birds and pigs, where the pigs are tired of boring grass and want eggs, either scrambled or sunny-side up. Thankfully, their neighbors, the birds, have a built-in egg production factory ripe for the taking. (Thus the angry.) The birds will do whatever it takes to get those eggs back, and besides a 90-second Looney Toons style video, thats all there is to the story.. Gameplay is simple enough: shoot birds with a slingshot at a selection of pigs, defended only by their glass, wooden or concrete surroundings. The angle you shoot them at makes all the difference, and once the bird is fired, physics takes over and drives the propelled ...
ROCK1 and LIMK2 interact in polarised cells.MDA-MB231 cells were microinjected with GFP-ROCK1 and mRFP-LIMK2, fixed, imaged and analysed using FLIM microscopy a
Beads, bacteria and actin. Cofilin promotes rapid actin filament turnover in vivo. Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
The SlingBucket" started life out as a Detroit Commerce body, custom built frame. Has halcraft spindle mount spoke wheels. Rolls suicide with Wilwood 4 piston calipers and 11" rotors out back. Has a built full manual 57 Chevy aluminum powerglide/shorty glide with 1.87 planetary and a JW ultrabell and a BossHogg 8" 3200 stall converter. Lincoln 9" with 33 spline axles steel yoke and coupler that uses two u-joints. Has Pete and Jakes ladder bars and 3800 air bags with panhard bar. Engine started life as a 240 and is the same block as a 300 but more nickle content. Has 300 based ratating internals but uses 390FE based piston and makes 10-1. Has arp head studs arp rods arp main studs. Rods are shot peened Crank is lightened and balanced under 0.5 grams. Cylinder head has 2.05 1.65 valves 7/16" screw in studs and big block Chevy 1.7 roller rockers with lash caps and 3/8" studs. Cam is a .574 lift 300 duration with 105 lobe separation. Engine has a steel gear set not phenolic. Intake is a ultra rare ...
This video shows that you dont have to choose between fishing and shooting your bow on any given day!. Click here to view this video by Fishing Video Feed.. ...
2-83. Coordinated Fire Line. A coordinated fire line (CFL) is a line beyond which conventional, direct, and indirect surface fire support means may fire at any time within the boundaries of the establishing headquarters without additional coordination. The purpose of the CFL is to expedite the surface-to-surface attack of targets beyond the CFL without coordination with the ground commander in whose area the targets are located (JP 3-09). Brigades or divisions usually establish a CFL, although a maneuver battalion may establish one. It is located as close as possible to the establishing unit without interfering with maneuver forces to open up the area beyond to fire support. A higher echelon may consolidate subordinate unit CFLs. If this occurs, any changes to the subordinate CFLs are coordinated with the subordinate headquarters. (See Figure 2-15.). 2-84. Fire Support Coordination Line. The fire support coordination line (FSCL) is a FSCM that is established and adjusted by appropriate land or ...
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This is one of the nerdier posts Ive done, but since Im unabashedly a nerd, and I hope many of you are too, I figured it would work.You see, I love LEDs. I think theyre fascinating in how they work, what they can do, and so on. As youd expect, Im slowly replacing the CFL bulbs in my house with LEDs.The thing is, not all LED bulbs are equal, and one of the biggest drawbacks is that not all offer the warmth in color temperature most of us love in incandescents. So I put a few different LEDs on my test bench, measuring them sort of how I measure TVs, to see how they do.Curious? Well I was, hopefully you will be too.
Anti-Phospho-Cofilin (S3) polyclonal antibody (STJ90230) was developed using a synthesized peptide derived from human Cofilin around the phosphorylation site of S3. This antibody is applicable for use in western blot, immunohistochemistry and ELISA protocol.
I was reading The Telegraph Magazine today [paper copy]. Salmon Rushdie introduces Taryn Simons An American Index of the Hidden and Unfamilar - a collection of portraits that capture the hidden reality: bushmeat, fruits and other items confiscated by US Customs; playboy Braille Edition; Transatlantic sub-marine cables reaching ashore and much more. Enthralling images, slingshots capable of sending us in a tangent, magical carpets of color which could transport us into a richer reality. ...
An 18-year-old from Bel Air is suspected in possibly 20 incidents of using a slingshot to fire small objects at homes and businesses over six days last week. ...
After germination planting 5 autos directly into 5 gallon bag. Which is better for Seedlings until i switch to 1000w MH?..LED, T5 or CFL? And what wattage...
Serotonin (5-HT) is known to increase the rate of growth cone advance via cofilin-dependent increases in retrograde actin network flow and nonmuscle myosin II activity ...
EDMONTON — Ricky Ray, who surpassed the 4,000-yard mark in passing yards for the season and the 40,000-yard plateau for his Eskimos career last weekend is Edmonton’s nominee for the CFL’s Most Outstanding Player, the club announced Wednesday.
D. A. Moshkov, I. Ya. Podolskii, L. N. Muzafarova, et al. "Quantitative characterization off motor activity in fish as a possible indicator of the state of Mauthner neurons,"Zh. fvol. Biokhim.,18, No. 2, 155-160 (1982).Google Scholar ...
Friday 1/10 Bench Press 225 lbs 2 x 2 Bench Press w/ Slingshot 255 lbs 2 x 2, 225 lbs x 6 Chest Supported Rows 100 lbs 4 x 8 Hammer Curls 32.5 lbers 3 x 12 So that closes out my first week of training for the powerlifting meet. Overall I feel pretty beat,…
We need to break this question down into two categories. The first would be manufacturers of CFLs that are dimmable and that screw into a standard socket and have an integral ballast. The other would be manufacturers that make hard-wire CFLs that have dimming ballasts within the housing.
This site portrays the most hottest happenings in life such as cine news, techie news, traditional news, automobile updates, photography and all the hot happenings around the world that keeps you interesting. TO BE INTERESTING , BE INTERESTED!!!. ...
CALGARY, ALBERTA--(Marketwired - April 28, 2016) - CERF Incorporated (the Company or CERF) (TSX VENTURE:CFL) today announced its financial and operating results for the year ended December 31, 2015 and the three months ended December 31, 2015. YEAR END FINANCIAL HIGHLIGHTS Amounts in the following tables are presented in thousands...
As of March 2017, during the lab retreat, the group is composed of Guillaume, Sandy, Yan, Emiko, Héliciane, Bérengère, Hugo, Antoine and Mikaël. ...
2004). "A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia". J. Cell Biol ... ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... 2007). "Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge". Cell. 128 (5): 915-29. doi:10.1016/j ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ...
... has been shown to interact with Cofilin 1. GRCh38: Ensembl release 89: ENSG00000120694 - Ensembl, May 2017 GRCm38: ... 869 (1-2): 49-55. doi:10.1016/S0006-8993(00)02346-5. PMID 10865058. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ... 1444 (1): 138-42. doi:10.1016/s0167-4781(98)00254-1. PMID 9931472. "Entrez Gene: HSPH1 heat shock 105kDa/110kDa protein 1". ... The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA ...
... signaling leads to phosphorylation of actin-interacting protein cofilin 1 at ser3; this may stabilize the actin ... "Reelin stabilizes the actin cytoskeleton of neuronal processes by inducing n-cofilin phosphorylation at serine3". The Journal ... 112 (1-2): 33-45. doi:10.1016/S0169-328X(03)00032-9. PMID 12670700. Hack I, Hellwig S, Junghans D, Brunne B, Bock HH, Zhao S, ... 165 (1-2): 1-9. doi:10.1016/j.psychres.2007.08.011. PMID 19054571. Suzuki K, Nakamura K, Iwata Y, Sekine Y, Kawai M, Sugihara G ...
In both yeast and mammals, CAPs appear to be involved in recycling G-actin monomers from ADF/cofilins for subsequent rounds of ... promotes cofilin-induced actin dynamics in mammalian nonmuscle cells". Mol. Biol. Cell. 15 (5): 2324-34. doi:10.1091/mbc.E04-01 ... 20 (1): 26-33. doi:10.1128/mcb.20.1.26-33.2000. PMC 85033 . PMID 10594005. Ksiazek D, Brandstetter H, Israel L, Bourenkov GP, ... Bertling E, Hotulainen P, Mattila PK, Matilainen T, Salminen M, Lappalainen P (May 2004). "Cyclase-associated protein 1 (CAP1) ...
ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... 2005). "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin". EMBO J. 24 (3): ... 2007). "The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and ...
Vallenius, T; Luukko K; Mäkelä T P (Apr 2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha- ... Otey CA, Pavalko FM, Burridge K (1990). "An interaction between alpha-actinin and the beta 1 integrin subunit in vitro". J. ... Vallenius T, Luukko K, Mäkelä TP (2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 ... An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin". Eur. J. Biochem. 268 (23): 6165-75. doi: ...
Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (Jun 1998). "Cofilin phosphorylation by LIM- ... Sumi T, Matsumoto K, Takai Y, Nakamura T (27 Dec 1999). "Cofilin phosphorylation and actin cytoskeletal dynamics regulated by ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (Aug 2001). "Cofilin phosphorylation and actin reorganization activities of ... Vardouli L, Moustakas A, Stournaras C (Mar 2005). "LIM-kinase 2 and cofilin phosphorylation mediate actin cytoskeleton ...
ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... a family of phosphatases that dephosphorylate ADF/cofilin". Cell. 108 (2): 233-46. doi:10.1016/S0092-8674(01)00638-9. PMID ... "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin". The EMBO Journal. 24 (3): ...
Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (1998). "Cofilin phosphorylation by LIM-kinase 1 ... Birkenfeld J, Betz H, Roth D (January 2003). "Identification of cofilin and LIM-domain-containing protein kinase 1 as novel ... Nebl G, Meuer SC, Samstag Y (1996). "Dephosphorylation of serine 3 regulates nuclear translocation of cofilin". J. Biol. Chem. ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of ...
Spratley SJ, Bastea LI, Döppler H, Mizuno K, Storz P (September 2011). "Protein kinase D regulates cofilin activity through p21 ... 349 (1): 45-50. doi:10.1016/j.canlet.2014.03.024. PMID 24704155. Park JK, Kim S, Han YJ, Kim SH, Kang NS, Lee H, Park S (June ... 317 (1): 24-32. doi:10.1016/j.canlet.2011.11.007. PMID 22085492. Yeo D, Huynh N, Beutler JA, Christophi C, Shulkes A, Baldwin ... 399 (1-2): 77-86. doi:10.1007/s11010-014-2234-9. PMID 25410752. Luo P, Fei J, Zhou J, Zhang W (May 2015). "microRNA-126 ...
Birkenfeld J, Betz H, Roth D (Jan 2003). "Identification of cofilin and LIM-domain-containing protein kinase 1 as novel ... 9 (1): 143-54. doi:10.1089/jam.1996.9.143. PMID 10160204. Check date values in: ,date= (help) Filoche M, Tai CF, Grotberg JB ( ... 369 (Pt 1): 45-54. doi:10.1042/BJ20021152. PMC 1223062 . PMID 12323073. Waterman MJ, Stavridi ES, Waterman JL, Halazonetis TD ( ... Koyama S, Williams LT, Kikuchi A (Jul 1995). "Characterization of the interaction of Raf-1 with ras p21 or 14-3-3 protein in ...
Enhanced docetaxel-mediated cytotoxicity in human prostate cancer cells through knockdown of cofilin-1 by carbon nanohorn ...
Toshima J, Toshima JY, Amano T, Yang N, Narumiya S, Mizuno K (2001). "Cofilin phosphorylation by protein kinase testicular ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of ... Dual specificity testis-specific protein kinase 1 is an enzyme that in humans is encoded by the TESK1 gene. This gene product ... "Entrez Gene: TESK1 testis-specific kinase 1". Toshima JY, Toshima J, Watanabe T, Mizuno K (November 2001). "Binding of 14-3- ...
... cofilin 1 MeSH D12.776.220.525.212.750 - cofilin 2 MeSH D12.776.220.525.212.875 - destrin MeSH D12.776.220.525.246.500 - actin- ... hiv-1 reverse transcriptase MeSH D12.776.964.970.880.325 - gene products, env MeSH D12.776.964.970.880.325.330 - hiv envelope ... neurofibromin 1 MeSH D12.776.402.150.500.500 - p120 gtpase activating protein MeSH D12.776.402.300.700.500 - ras-GRF1 MeSH ... hiv-1 reverse transcriptase MeSH D12.776.964.775.750.320 - fusion proteins, gag-onc MeSH D12.776.964.775.750.320.700 - oncogene ...
... cofilin 2 MeSH D05.750.078.730.212.875 --- destrin MeSH D05.750.078.730.246 --- actin-related protein 2-3 complex MeSH D05.750. ...
The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of ... Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever ... Destrin binds actin and is thought to connect it as gelsolin segment-1 does. ...
... or n-cofilin) CFL2, coding for cofilin 2 (found in muscle: m-cofilin) DSTN, coding for destrin, also known as ADF or actin ... Cofilin, a member of the ADF/cofilin family is actually a protein with 70% sequence identity to ADF, making it part of the ADF/ ... Cofilin is accommodated in ADP-F actin because of increased flexibility in this form of actin. Binding by both cofilin and ADF( ... Cofilin is a ubiquitous actin-binding factor required for the reorganization of actin filaments. ADF/Cofilin family members ...
Cofilin 2 (muscle) also known as CFL2 is a protein which in humans is encoded by the CFL2 gene. Cofilin is a widely distributed ... Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN), all of which regulate ... "Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14". Ann. Hum ... 2006). "Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface". J. Mol. Biol. ...
... n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular ... Lee K, Jung J, Kim M, Guidotti G (2001). "Interaction of the alpha subunit of Na,K-ATPase with cofilin". Biochem. J. 353 (Pt 2 ... Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 ... Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (1998). "Cofilin phosphorylation by LIM-kinase 1 ...
In accordance with their proposed rule, proteins like ADF, cofilin and LIMK1 are slingshot substrates. Phosphatases of ... Regenerating Liver (PRLs): Three PRL genes were described in mammals (PRL-1, PRL-2 and PRL-3). They share a high sequence ...
The latter is the enzyme that uses ATP to phosphorylate and inactivate the actin-depolymerizing factor cofilin. When cofilin is ... In the presence of Pyr1, LIMK1 is inhibited, which means that the phosphorylation of cofilin decreases, which results in the ... Pyr 1 is a cell permeable competitive inhibitor of Lim Kinase (especially LIMK1). ...
ADF/cofilin are the only substrates yet identified for the LIM kinases. LIM kinases directly phosphorylate and inactivate ... are actin-binding kinases that phosphorylate members of the ADF/cofilin family of actin binding and filament severing proteins ... LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal ... members of the cofilin family, resulting in stabilization of filamentous (F)-actin. Lim kinases are activated by signaling ...
Meanwhile, phosphorylation of PI3K by SRC activates RhoA which leads to activation of cofilin-P and disruption of filamentous ... When the WAVE-1 gene was disurpted in mice, it resulted in cognitive defects such as losses in learning and memory implicating ... 28 (1): 245-259. doi:10.1016/S0896-6273(00)00100-8. PMID 11086998. Tanaka, H; Shan, W; Phillips, GR; Arndt, K; Bozdagi, O; ... 51 (1): 43-56. doi:10.1016/j.neuron.2006.05.018. PMC 2587166 . PMID 16815331. Ohkubo, T; Ozawa, M. (1999). "p120ctn Binds to ...
... which then stimulates cofilin, which effectively reorganizes the actin cytoskeleton of the cell. In the case of neurons, ... The LINGO-1 structure has been shown to be highly stable both in its crystal form and in solution, thanks to its leucine-rich ... LINGO-1 is coded by the LINGO-1 gene, which is located on the human chromosome 15, more precisely on the locus 15q24-26, which ... LINGO-1 is expressed almost exclusively in the central nervous system (CNS). It can be found in the brain and in neurons and ...
2005). "Ribosomal protein S18 identified as a cofilin-binding protein by using phage display library". Mol. Cell. Biochem. 262 ... 239 (1): 144-9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID 8706699. Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed ... 12 (1): 1-11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. Mishra-Gorur K, Singer HA, Castellot JJ (2002). "The S18 ... 1-2): 187-93. doi:10.1023/B:MCBI.0000038234.35936.1c. PMID 15532723. Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar ...
核纤层蛋白 A/C(英语:Lamin A/C)是由人类基因LMNA 编码的蛋白质[1][2],属于核纤层蛋白家族。 ... 2000, 177 (1): 1-11. PMID 10960149. doi:10.1007/s002320001096.. *. Burke B, Mounkes LC, Stewart CL. The nuclear envelope in ... 2007, 53 (1): 46-52. PMID 17718387.. *. Genschel J, Schmidt HH. Mutations in the LMNA gene encoding lamin A/C. Hum. Mutat. ... Type 1/2
PF00241 Cofilin_ADF, 1 hit. PIRSFi. PIRSF001788 GMF-beta, 1 hit. ... Q63228-1 [UniParc]FASTAAdd to basketAdded to basket. « Hide. 10 20 30 40 50. MSESLVVCDV AEDLVEKLRK FRFRKETHNA AIIMKIDKDK ... Removed1 Publication. ,p>Manually curated information for which there is published experimental evidence.,/p> ,p>,a href="/ ... There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket. ...
8). Cofilin is phosphorylated during long-term potentiation, and it has been suggested that cofilin phosphorylation induces an ... Rif expression or cofilin-1 siRNA transfections caused only a slight reduction to 0.26 per micrometer (P = 0.326) or 0.25 per ... Similarly, depletion of cofilin-1 led to a decrease in the number of thin, mushroom, and stubby spines, although the defects ... Cofilin-1 is necessary for proper spine morphology and actin turnover. (A) Mouse hippocampal neurons were transfected with GFP ...
Using RNAi, we were then able to define roles for cofilin and phosphorylated cofilin (P-cofilin) in the response of S2R+ cells ... Cells were lysed and P-cofilin and β-actin levels assessed in the lysates by immunoblotting. (B) Immunoblotting of P-cofilin, P ... 6D). This confirms the link between actin filaments and P-cofilin, and shows that free cofilin can serve as a substrate for ... B) The relative abundances of P-cofilin (acidic isoform) and cofilin (basic isoform) are shown for RNAi-treated cells from 2D- ...
The data obtained demonstrated that mDia1-FH2 induced actin repolymerization only from the actin/cofilin-1 complex, but not ... The data obtained demonstrated that mDia1-FH2 induced actin repolymerization only from the actin/cofilin-1 complex, but not ... The data obtained demonstrated that mDia1-FH2 induced actin repolymerization only from the actin/cofilin-1 complex, but not ... The data obtained demonstrated that mDia1-FH2 induced actin repolymerization only from the actin/cofilin-1 complex, but not ...
The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of ... Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever ... Destrin binds actin and is thought to connect it as gelsolin segment-1 does. ...
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
... n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular ... Lee K, Jung J, Kim M, Guidotti G (2001). "Interaction of the alpha subunit of Na,K-ATPase with cofilin". Biochem. J. 353 (Pt 2 ... Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 ... Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (1998). "Cofilin phosphorylation by LIM-kinase 1 ...
Homo sapiens cofilin pseudogene 1, mRNA (cDNA clone IMAGE:5168640). A. NR_028492.1. Homo sapiens cofilin 1 (non-muscle) ... Cofilin 1 (non-muscle) pseudogene 1 (CFL1P1). Human pseudo gene CFL1P1. Represented by 16 ESTs from 10 cDNA libraries. ...
"Determining the differences in actin binding by human ADF and cofilin.". Yeoh S., Pope B., Mannherz H.G., Weeds A.. J. Mol. ... "Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family.". Nakano K., Kanai ... "Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family.". Nakano K., Kanai ... "Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics.". Gohla A., Birkenfeld J. ...
The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and therefore potentially oncogenic missense mutations (click on Show Cancer Mutations). The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. ...
Both the cofilin-inhibitory peptides (Eibert et al., 2004) and cofilin S3D (Elam et al., 2015) bind F-actin and prevent ... Cofilin is activated by dephosphorylation of serine 3 (S3) (Mizuno, 2013). To assess the role of cofilin-mediated F-actin ... Rap1-GTP promotes cofilin dephosphorylation and stimulates cofilin-mediated actin severing. The resulting clearance of F-actin ... 3J,K). The ability of activated cofilin to bypass a defect in Rap1 activation argues that cofilin is the major downstream ...
"Cofilin-1 phosphorylation catalyzed by ERK1/2 alters cardiac actin dynamics in dilated cardiomyopathy caused by lamin A/C gene ... 4C). Expression of cofilin-2 in the heart was unchanged by overexpression of cofilin-1 or cofilin-1-T25A (Fig. 4C). Expression ... 4C). Expression of cofilin-2 in the heart was unchanged by overexpression of cofilin-1 or cofilin-1-T25A (Fig. 4C). Expression ... 4A). Expression of cofilin-2 in the heart was unchanged by over-expression of cofilin-1 (Fig. 4A). There was depolymerization ...
The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse ... The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse ... The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse ... The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse ...
Liu L, Li J, Zhang L, Zhang F, Zhang R, Chen X, Brakebusch C, Wang Z, Liu X (2015) Cofilin phosphorylation is elevated after F‐ ... Mizuno K, Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E (1998) Cofilin phosphorylation by LIM‐kinase 1 ... induced activation of cofilin‐phosphatase Slingshot and cofilin in lamellipodia. J Cell Biol 165: 465-471. ... Upstream of cofilin1, signalling from the RHO GTPase and the ROCK1 and LIMK1 kinases controls cofilin1 activity to remodel ...
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and ... It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004] ...
Dichotomous effects of the cofilin kinase LIMK1 on the early steps of HIV-1 infection of CD4 T cells. ... directly phosphorylates cofilin and regulates the actin cytoskeleton. Here, we investigated the role of LIMK1 in HIV-1 ... on resting CD4 T cells activates an actin-depolymerizing factor cofilin to promote the cortical actin dynamic critical for HIV ... HIV envelope-CXCR4 signaling activates cofilin to overcome cortical actin restriction in resting CD4 T cells. Cell. 134: 782-92 ...
In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2) ... Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such ... In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ... Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts ...
HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1. Proceedings ... HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1. / Quintela- ... title = "HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1", ... T1 - HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1 ...
Cofilin 1 Pseudogene 8, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human ... CFL1P8 (Cofilin 1 Pseudogene 8) is a Pseudogene, and is affiliated with the lncRNA class. ... 1) Ensembl transcripts including schematic representations, and UCSC links where relevant :. * ENST00000436148 475 bps ...
Cofilin-1: A Modulator of Anxiety in Mice Martin Goodson, Marco B. Rust, Walter Witke, David Bannerman, Richard Mott, Chris P. ... Splicing Factors ASD-2 and SUP-12 Cooperatively Switch Alternative Pre-mRNA Processing Patterns of the ADF/Cofilin Gene in ... 1 segregation pattern called gene conversion. In this issue, Sun et al. use a visual, pollen tetrad-based assay to measure the ...
Cofilin was used to normalize protein loading. D , E , Densitometric analysis of cofilin and LIMK1 phosphorylation in tissue ... Reelin-induced phosphorylation of n-cofilin involves Dab1, PI3K, and ApoER2. A , Reelin upregulates p-cofilin in E17.5 dab1 +/− ... Reelin increases n-cofilin phosphorylation in cultured hippocampal neurons. To visualize Reelin-induced n-cofilin ... The staining for serine3 phospho-cofilin in the marginal zone of reeler mutants suggests that cofilin may be phosphorylated by ...
... or n-cofilin) CFL2, coding for cofilin 2 (found in muscle: m-cofilin) DSTN, coding for destrin, also known as ADF or actin ... Cofilin, a member of the ADF/cofilin family is actually a protein with 70% sequence identity to ADF, making it part of the ADF/ ... Cofilin is accommodated in ADP-F actin because of increased flexibility in this form of actin. Binding by both cofilin and ADF( ... Cofilin is a ubiquitous actin-binding factor required for the reorganization of actin filaments. ADF/Cofilin family members ...
immunogen = synthetic peptide: C-D I K D R S T L G E K, representing the C-terminus of the human protein according to NP_068733.1; NP_619579.1. ...
2000 Antifreeze Proteins by Bill Nye for National Science Foundation.oga 1 min 3 s; 508 KB. ... 2ph1 pdb gallery nucleotide binding-protein-2 (MinD homolog).svg 1 101 × 745; 302 KB. ... 2L6X Active Site Visualization E.Vitureira.png 1 054 × 770; 275 KB. ... У гэтай катэгорыі ёсць наступныя 200 файлаў з агульнага ліку 1 125. ...
Non-overlapping activities of ADF and cofilin-1 during the migration of metastatic breast tumor cells. . Biblioteca virtual ... Focal adhesion area and cell adhesion in cofilin KD cells were returned to control levels by expressing exogenous cofilin but ... Non-overlapping activities of ADF and cofilin-1 during the migration of metastatic breast tumor cells - Lubna H Tahtamouni - ... whereas in cofilin KD cells, expression of cofilin efficiently rescued control migration rates.. ConclusionAlthough ADF and ...
  • Taken together, our data suggest that the dynamic regulation of cofilin activity acts as a feedback control mechanism, which monitors the level of actin filaments and helps to restore the resting state of the cytoskeleton following acute signalling. (biologists.org)
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