Cofilin 1. Medical search

Cofilin 1 is a member of the cofilin family of proteins that is expressed in non-muscle CELLS. It has ACTIN depolymerization activity that is dependent on HYDROGEN-ION CONCENTRATION.

A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. They remodel the actin CYTOSKELETON by severing ACTIN FILAMENTS and increasing the rate of monomer dissociation.

A member of the cofilin family of proteins that is expressed in MUSCLE CELLS. It has ACTIN depolymerization activity that is dependent on HYDROGEN-ION CONCENTRATION.

Serine protein kinases involved in the regulation of ACTIN polymerization and MICROTUBULE disassembly. Their activity is regulated by phosphorylation of a threonine residue within the activation loop by intracellular signaling kinases such as P21-ACTIVATED KINASES and by RHO KINASE.

A member of the actin depolymerizing factors. Its depolymerizing activity is independent of HYDROGEN-ION CONCENTRATION.

Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.

Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.

Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.

A dynamic actin-rich extension of the surface of an animal cell used for locomotion or prehension of food.

The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

A family of low molecular weight proteins that bind ACTIN and control actin polymerization. They are found in eukaryotes and are ubiquitously expressed.

A 90-kDa protein produced by macrophages that severs ACTIN filaments and forms a cap on the newly exposed filament end. Gelsolin is activated by CALCIUM ions and participates in the assembly and disassembly of actin, thereby increasing the motility of some CELLS.

A family of serine-threonine kinases that bind to and are activated by MONOMERIC GTP-BINDING PROTEINS such as RAC GTP-BINDING PROTEINS and CDC42 GTP-BINDING PROTEIN. They are intracellular signaling kinases that play a role the regulation of cytoskeletal organization.

Specialized structures of the cell that extend the cell membrane and project out from the cell surface.

A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments.

Very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of liver damage.

The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.

1,N-6-Ethenoadenosine triphosphate. A fluorescent analog of adenosine triphosphate.

A group of intracellular-signaling serine threonine kinases that bind to RHO GTP-BINDING PROTEINS. They were originally found to mediate the effects of rhoA GTP-BINDING PROTEIN on the formation of STRESS FIBERS and FOCAL ADHESIONS. Rho-associated kinases have specificity for a variety of substrates including MYOSIN-LIGHT-CHAIN PHOSPHATASE and LIM KINASES.

A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. It is expressed at higher levels than ARP2 PROTEIN and does not contain a PROFILIN binding domain.

The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.

Reduced (protonated) form of THIAZOLES. They can be oxidized to THIAZOLIDINEDIONES.

Polymers synthesized by living organisms. They play a role in the formation of macromolecular structures and are synthesized via the covalent linkage of biological molecules, especially AMINO ACIDS; NUCLEOTIDES; and CARBOHYDRATES.

A PROFILIN binding domain protein that is part of the Arp2-3 complex. It is related in sequence and structure to ACTIN and binds ATP.

A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)

Chemical reaction in which monomeric components are combined to form POLYMERS (e.g., POLYMETHYLMETHACRYLATE).

An actin capping protein that binds to the barbed-ends of ACTIN filaments. It is a heterodimer consisting of an alpha and a beta subunit. It regulates actin assembly by stabilizing actin oligomers for elongation. In SKELETAL MUSCLE, CapZ is localized to the Z-disk.

A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.

A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47.

Spiny processes on DENDRITES, each of which receives excitatory input from one nerve ending (NERVE ENDINGS). They are commonly found on PURKINJE CELLS and PYRAMIDAL CELLS.

Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.

The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.

Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.

Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.

Actin capping proteins are cytoskeletal proteins that bind to the ends of ACTIN FILAMENTS to regulate actin polymerization.

A class of saturated compounds consisting of two rings only, having two or more atoms in common, containing at least one hetero atom, and that take the name of an open chain hydrocarbon containing the same total number of atoms. (From Riguady et al., Nomenclature of Organic Chemistry, 1979, p31)

A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.

Bundles of actin filaments (ACTIN CYTOSKELETON) and myosin-II that span across the cell attaching to the cell membrane at FOCAL ADHESIONS and to the network of INTERMEDIATE FILAMENTS that surrounds the nucleus.

A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)

A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.

A factor identified in the brain that influences the growth and differentiation of NEURONS and NEUROGLIA. Glia maturation factor beta is the 17-kDa polypeptide product of the GMFB gene and is the principal component of GLIA MATURATION FACTOR.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

Bulbous enlargement of the growing tip of nerve axons and dendrites. They are crucial to neuronal development because of their pathfinding ability and their role in synaptogenesis.

The quality of surface form or outline of ORGANELLES.

The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.

Structures which are part of the CELL MEMBRANE or have cell membrane as a major part of their structure.

An immunoassay utilizing an antibody labeled with an enzyme marker such as horseradish peroxidase. While either the enzyme or the antibody is bound to an immunosorbent substrate, they both retain their biologic activity; the change in enzyme activity as a result of the enzyme-antibody-antigen reaction is proportional to the concentration of the antigen and can be measured spectrophotometrically or with the naked eye. Many variations of the method have been developed.

A factor synthesized in a wide variety of tissues. It acts synergistically with TGF-alpha in inducing phenotypic transformation and can also act as a negative autocrine growth factor. TGF-beta has a potential role in embryonal development, cellular differentiation, hormone secretion, and immune function. TGF-beta is found mostly as homodimer forms of separate gene products TGF-beta1, TGF-beta2 or TGF-beta3. Heterodimers composed of TGF-beta1 and 2 (TGF-beta1.2) or of TGF-beta2 and 3 (TGF-beta2.3) have been isolated. The TGF-beta proteins are synthesized as precursor proteins.

All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.

A subtype of transforming growth factor beta that is synthesized by a wide variety of cells. It is synthesized as a precursor molecule that is cleaved to form mature TGF-beta 1 and TGF-beta1 latency-associated peptide. The association of the cleavage products results in the formation a latent protein which must be activated to bind its receptor. Defects in the gene that encodes TGF-beta1 are the cause of CAMURATI-ENGELMANN SYNDROME.

Cell-surface proteins that bind transforming growth factor beta and trigger changes influencing the behavior of cells. Two types of transforming growth factor receptors have been recognized. They differ in affinity for different members of the transforming growth factor beta family and in cellular mechanisms of action.

A preparation consisting of PLATELETS concentrated in a limited volume of PLASMA. This is used in various surgical tissue regeneration procedures where the GROWTH FACTORS in the platelets enhance wound healing and regeneration.

Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.

The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics. (1/145)

Actin-depolymerizing factor (ADF)/cofilins are essential regulators of actin filament turnover. Several ADF/cofilin isoforms are found in multicellular organisms, but their biological differences have remained unclear. Herein, we show that three ADF/cofilins exist in mouse and most likely in all other mammalian species. Northern blot and in situ hybridization analyses demonstrate that cofilin-1 is expressed in most cell types of embryos and adult mice. Cofilin-2 is expressed in muscle cells and ADF is restricted to epithelia and endothelia. Although the three mouse ADF/cofilins do not show actin isoform specificity, they all depolymerize platelet actin filaments more efficiently than muscle actin. Furthermore, these ADF/cofilins are biochemically different. The epithelial-specific ADF is the most efficient in turning over actin filaments and promotes a stronger pH-dependent actin filament disassembly than the two other isoforms. The muscle-specific cofilin-2 has a weaker actin filament depolymerization activity and displays a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1 and ADF. In steady-state assays, cofilin-2 also promotes filament assembly rather than disassembly. Taken together, these data suggest that the three biochemically distinct mammalian ADF/cofilin isoforms evolved to fulfill specific requirements for actin filament dynamics in different cell types. (+info)

The human D2 dopamine receptor synergizes with the A2A adenosine receptor to stimulate adenylyl cyclase in PC12 cells. (2/145)

The adenosine A(2A) receptor and the dopamine D(2) receptor are prototypically coupled to G(s) and G(i)/G(o), respectively. In striatal intermediate spiny neurons, these receptors are colocalized in dendritic spines and act as mutual antagonists. This antagonism has been proposed to occur at the level of the receptors or of receptor-G protein coupling. We tested this model in PC12 cells which endogenously express A(2A) receptors. The human D(2) receptor was introduced into PC12 cells by stable transfection. A(2A)-agonist-mediated inhibition of D(2) agonist binding was absent in PC12 cell membranes but present in HEK293 cells transfected as a control. However, in the resulting PC12 cell lines, the action of the D(2) agonist quinpirole depended on the expression level of the D(2) receptor: at low and high receptor levels, the A(2A)-agonist-induced elevation of cAMP was enhanced and inhibited, respectively. Forskolin-stimulated cAMP formation was invariably inhibited by quinpirole. The effects of quinpirole were abolished by pretreatment with pertussis toxin. A(2A)-receptor-mediated cAMP formation was inhibited by other G(i)/G(o)-coupled receptors that were either endogenously present (P(2y12)-like receptor for ADP) or stably expressed after transfection (A(1) adenosine, metabotropic glutamate receptor-7A). Similarly, voltage activated Ca(2+) channels were inhibited by the endogenous P(2Y) receptor and by the heterologously expressed A(1) receptor but not by the D(2) receptor. These data indicate functional segregation of signaling components. Our observations are thus compatible with the proposed model that D(2) and A(2A) receptors are closely associated, but they highlight the fact that this interaction can also support synergism. (+info)

Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. (3/145)

Actin-depolymerizing factor (ADF)/cofilins are small actin-binding proteins found in all eukaryotes. In vitro, ADF/cofilins promote actin dynamics by depolymerizing and severing actin filaments. However, whether ADF/cofilins contribute to actin dynamics in cells by disassembling "old" actin filaments or by promoting actin filament assembly through their severing activity is a matter of controversy. Analysis of mammalian ADF/cofilins is further complicated by the presence of multiple isoforms, which may contribute to actin dynamics by different mechanisms. We show that two isoforms, ADF and cofilin-1, are expressed in mouse NIH 3T3, B16F1, and Neuro 2A cells. Depleting cofilin-1 and/or ADF by siRNA leads to an accumulation of F-actin and to an increase in cell size. Cofilin-1 and ADF seem to play overlapping roles in cells, because the knockdown phenotype of either protein could be rescued by overexpression of the other one. Cofilin-1 and ADF knockdown cells also had defects in cell motility and cytokinesis, and these defects were most pronounced when both ADF and cofilin-1 were depleted. Fluorescence recovery after photobleaching analysis and studies with an actin monomer-sequestering drug, latrunculin-A, demonstrated that these phenotypes arose from diminished actin filament depolymerization rates. These data suggest that mammalian ADF and cofilin-1 promote cytoskeletal dynamics by depolymerizing actin filaments and that this activity is critical for several processes such as cytokinesis and cell motility. (+info)

Maternal diet programs embryonic kidney gene expression. (4/145)

Human epidemiological data associating birth weight with adult disease suggest that organogenesis is "programmed" by maternal diet. In rats, protein restriction in pregnancy produces offspring with fewer renal glomeruli and higher systemic blood pressures than controls. We tested the hypothesis that maternal diet alters gene expression in the metanephros, the precursor of the definitive mammalian kidney. We demonstrated that maternal low-protein diet initiated when pregnancy starts and maintained to embryonic day 13, when the metanephros consists of mesenchyme surrounding a once-branched ureteric bud, is sufficient to significantly reduce glomerular numbers in offspring by about 20%. As assessed by representational difference analyses and real-time quantitative polymerase chain reactions, low-protein diet modulated gene expression in embryonic day 13 metanephroi. In particular, levels of prox-1, the ortholog of Drosophila transcription factor prospero, and cofilin-1, a regulator of the actin cytoskeleton, were reduced. During normal metanephrogenesis, prox-1 protein was first detected in mesenchymal cells around the ureteric tree and thereafter in nascent nephron epithelia, whereas cofilin-1 immunolocalized to bud derivatives and condensing mesenchyme. Previously, we reported that low-protein diets increased mesenchymal apoptosis cells when metanephrogenesis began and thereafter reduced numbers of precursor cells. Collectively, these studies prove that the maternal diet programs the embryonic kidney, altering cell turnover and gene expression at a time when nephrons and glomeruli have yet to form. The human implication is that the maternal diet ingested between conception and 5- 6-wk gestation contributes to the variation in glomerular numbers that are known to occur between healthy and hypertensive populations. (+info)

Micropuncture gene delivery and intravital two-photon visualization of protein expression in rat kidney. (5/145)

Understanding molecular mechanisms of pathophysiology and disease processes requires the development of new methods for studying proteins in animal tissues and organs. Here, we describe a method for adenoviral-mediated gene transfer into tubule or endothelial cells of the rat kidney. The left kidney of an anesthetized rat was exposed and the lumens of superficial proximal tubules or vascular welling points were microinfused, usually for 20 min. The microinfusion solution contained adenovirus with a cDNA construct of either 1) Xenopus laevis actin depolymerizing factor/cofilin [XAC; wt-green fluorescent protein (GFP)], 2) actin-GFP, or 3) GFP. Sudan black-stained castor oil, injected into nearby tubules, allowed us to localize the microinfused structures for subsequent visualization. Two days later, the rat was anesthetized and the kidneys were fixed for tissue imaging or the left kidney was observed in vivo using two-photon microscopy. Expression of GFP and GFP-chimeric proteins was clearly seen in epithelial cells of the injected proximal tubules and the expressed proteins were localized similarly to their endogenously expressed counterparts. Only a minority of the cells in the virally exposed regions, however, expressed these proteins. Endothelial cells also expressed XAC-GFP after injection of the virus cDNA construct into vascular welling points. An advantage of the proximal tubule and vascular micropuncture approaches is that only minute amounts of virus are required to achieve protein expression in vivo. This micropuncture approach to gene transfer of the virus cDNA construct and intravital two-photon microscopy should be applicable to study of the behavior of any fluorescently tagged protein in the kidney and shows promise in studying renal physiology and pathophysiology. (+info)

Ligation of cell surface-associated glucose-regulated protein 78 by receptor-recognized forms of alpha 2-macroglobulin: activation of p21-activated protein kinase-2-dependent signaling in murine peritoneal macrophages. (6/145)

Previous studies of the plasma proteinase inhibitor alpha2-macroglobulin (alpha2M) demonstrated that alpha2M-proteinase complexes (alpha2M*) modulate immune responses and promotes macrophage locomotion and chemotaxis. Alpha2M* binds to cell surface-associated glucose-regulated protein 78 (GRP78), which activates downstream signaling events. The role of p21-activated protein kinase-1 and -2 (PAK-1 and -2) in promoting cellular motility is well documented. In the current study, we examined the ability of alpha2M* to activate PAK-1 and PAK-2. Upon macrophage stimulation with alpha2M*, PAK-2 is autophosphorylated, resulting in increased kinase activity; however, PAK-1 is negligibly affected. Alpha2M*-stimulated macrophages showed a marked elevation in the levels of Rac x GTP. Receptor tyrosine phosphorylation upon binding of alpha2M* to GRP78, recruits PAK-2 to the plasma membrane via the adaptor protein NCK. Consistent with this hypothesis, silencing of GRP78 gene expression greatly attenuated the levels of membrane-associated PAK-2 and NCK. PAK-2 activity was markedly decreased by inhibition of tyrosine kinases and PI3K before alpha2M* stimulation. We further demonstrate that phosphorylation of Lin-11, Isl-1, Mec-3 (LIM) kinase and cofilin is promoted by treating macrophages with alpha2M*. Thus, alpha2M* regulates activation of the PAK-2-dependent motility mechanism in these cells. (+info)

Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C. (7/145)

BACKGROUND: Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS: A method for DNA splicing and mutagenesis without restriction enzymes is described. The method is based on mild template-dependent polymerization arrest with two molecules of cytosine arabinose (Ara-C) incorporated into PCR primers. Two rounds of PCR are employed: the first PCR produces 5' overhangs that are utilized for DNA splicing. The second PCR is based on polymerization running through the Ara-C molecules to produce the desired final product. To illustrate application of the run through stop mutagenesis and DNA splicing technique, we have carried out splicing of two segments of the human cofilin 1 gene and introduced a mutational deletion into the product. CONCLUSION: We have demonstrated the utility of a new PCR-based method for carrying out DNA splicing and mutagenesis by incorporating Ara-C into the PCR primers. (+info)

Stimulation of actin polymerization by filament severing. (8/145)

The extent and dynamics of actin polymerization in solution are calculated as functions of the filament severing rate, using a simple model of in vitro polymerization. The model is solved by both analytic theory and stochastic-growth simulation. The results show that severing essentially always enhances actin polymerization by freeing up barbed ends, if barbed-end cappers are present. Severing has much weaker effects if only pointed-end cappers are present. In the early stages of polymerization, the polymerized-actin concentration grows exponentially as a function of time. The exponential growth rate is given in terms of the severing rate, and the latter is given in terms of the maximum slope in a polymerization time course. Severing and branching are found to act synergistically. (+info)

... n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular ... "Entrez Gene: CFL1 cofilin 1 (non-muscle)". Chai X, Förster E, Zhao S, Bock HH, Frotscher M (January 2009). "Reelin stabilizes ... Lee K, Jung J, Kim M, Guidotti G (2001). "Interaction of the alpha subunit of Na,K-ATPase with cofilin". Biochem. J. 353 (Pt 2 ... Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 ...

https://en.wikipedia.org/wiki/Cofilin_1

Cofilin 2 (muscle) also known as CFL2 is a protein which in humans is encoded by the CFL2 gene. Cofilin is a widely distributed ... Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN), all of which regulate ... "Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14". Ann. Hum ... 2006). "Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface". J. Mol. Biol. ...

https://en.wikipedia.org/wiki/Cofilin-2

... has been shown to interact with Cofilin 1. GRCh38: Ensembl release 89: ENSG00000120694 - Ensembl, May 2017 GRCm38: ... 869 (1-2): 49-55. doi:10.1016/S0006-8993(00)02346-5. PMID 10865058. S2CID 7179171. Strausberg RL, Feingold EA, Grouse LH, et al ... 1444 (1): 138-42. doi:10.1016/s0167-4781(98)00254-1. PMID 9931472. "Entrez Gene: HSPH1 heat shock 105kDa/110kDa protein 1". ... The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA ...

https://en.wikipedia.org/wiki/HSPH1

... signaling leads to phosphorylation of actin-interacting protein cofilin 1 at ser3; this may stabilize the actin ... "Reelin stabilizes the actin cytoskeleton of neuronal processes by inducing n-cofilin phosphorylation at serine3". The Journal ... 112 (1-2): 33-45. doi:10.1016/S0169-328X(03)00032-9. PMID 12670700. Hack I, Hellwig S, Junghans D, Brunne B, Bock HH, Zhao S, ... 1 (2): 72-73. doi:10.1046/j.1535-7597.2001.00017.x. PMC 320825. PMID 15309195. Zaki M, Shehab M, El-Aleem AA, Abdel-Salam G, ...

https://en.wikipedia.org/wiki/Reelin

In both yeast and mammals, CAPs appear to be involved in recycling G-actin monomers from ADF/cofilins for subsequent rounds of ... Moriyama K, Yahara I (April 2002). "Human CAP1 is a key factor in the recycling of cofilin and actin for rapid actin turnover ... A second unique function of CAP in regulating actin dynamics is its ability to specifically bind to pointed ends of cofilin- ... Shekhar S, Chung J, Kondev J, Gelles J, Goode BL (22 November 2019). "Synergy between Cyclase-associated protein and Cofilin ...

https://en.wikipedia.org/wiki/Cyclase-associated_protein_family

ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... 2005). "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin". EMBO J. 24 (3): ... 2007). "The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and ...

https://en.wikipedia.org/wiki/SSH2

Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (1998). "Cofilin phosphorylation by LIM-kinase 1 ... Birkenfeld J, Betz H, Roth D (January 2003). "Identification of cofilin and LIM-domain-containing protein kinase 1 as novel ... Nebl G, Meuer SC, Samstag Y (1996). "Dephosphorylation of serine 3 regulates nuclear translocation of cofilin". J. Biol. Chem. ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of ...

https://en.wikipedia.org/wiki/LIMK1

Vallenius, T; Luukko K; Mäkelä T P (Apr 2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha- ... Otey CA, Pavalko FM, Burridge K (1990). "An interaction between alpha-actinin and the beta 1 integrin subunit in vitro". J. ... Vallenius T, Luukko K, Mäkelä TP (2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 ... An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin". Eur. J. Biochem. 268 (23): 6165-75. doi: ...

https://en.wikipedia.org/wiki/Alpha-actinin-4

Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (Jun 1998). "Cofilin phosphorylation by LIM- ... Sumi T, Matsumoto K, Takai Y, Nakamura T (27 Dec 1999). "Cofilin phosphorylation and actin cytoskeletal dynamics regulated by ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (Aug 2001). "Cofilin phosphorylation and actin reorganization activities of ... Vardouli L, Moustakas A, Stournaras C (Mar 2005). "LIM-kinase 2 and cofilin phosphorylation mediate actin cytoskeleton ...

https://en.wikipedia.org/wiki/LIMK2

ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... a family of phosphatases that dephosphorylate ADF/cofilin". Cell. 108 (2): 233-46. doi:10.1016/S0092-8674(01)00638-9. PMID ... "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin". The EMBO Journal. 24 (3): ...

https://en.wikipedia.org/wiki/SSH3

2004). "A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia". J. Cell Biol ... ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... 2007). "Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge". Cell. 128 (5): 915-29. doi:10.1016/j ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ...

https://en.wikipedia.org/wiki/SSH1

"Prion-like protein aggregates exploit the RHO GTPase to cofilin-1 signaling pathway to enter cells". The EMBO Journal. 37 (6). ... One of the inhibitors discovered in Bertolotti's lab, Sephin1, has passed through favourable Phase 1 clinical trials in 2019 ... Münch, Christian; O'Brien, John; Bertolotti, Anne (2011-03-01). "Prion-like propagation of mutant superoxide dismutase-1 ... "Exposure of hydrophobic surfaces initiates aggregation of diverse ALS-causing superoxide dismutase-1 mutants". Journal of ...

https://en.wikipedia.org/wiki/Anne_Bertolotti

Spratley SJ, Bastea LI, Döppler H, Mizuno K, Storz P (September 2011). "Protein kinase D regulates cofilin activity through p21 ... 349 (1): 45-50. doi:10.1016/j.canlet.2014.03.024. PMID 24704155. Park JK, Kim S, Han YJ, Kim SH, Kang NS, Lee H, Park S (June ... 317 (1): 24-32. doi:10.1016/j.canlet.2011.11.007. PMID 22085492. Yeo D, Huynh N, Beutler JA, Christophi C, Shulkes A, Baldwin ... 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. Dan C, Nath N, Liberto M, Minden A (January 2002). "PAK5, a new ...

https://en.wikipedia.org/wiki/PAK4

Birkenfeld J, Betz H, Roth D (January 2003). "Identification of cofilin and LIM-domain-containing protein kinase 1 as novel ... 9 (1): 143-54. doi:10.1089/jam.1996.9.143. PMID 10160204. Filoche M, Tai CF, Grotberg JB (July 2015). "Three-dimensional model ... 369 (Pt 1): 45-54. doi:10.1042/BJ20021152. PMC 1223062. PMID 12323073. Waterman MJ, Stavridi ES, Waterman JL, Halazonetis TD ( ... Koyama S, Williams LT, Kikuchi A (July 1995). "Characterization of the interaction of Raf-1 with ras p21 or 14-3-3 protein in ...

https://en.wikipedia.org/wiki/YWHAZ

Enhanced docetaxel-mediated cytotoxicity in human prostate cancer cells through knockdown of cofilin-1 by carbon nanohorn ... 1 July 2016). "Design of Assembled Systems Based on Conjugated Polyphenylene Derivatives and Carbon Nanohorns". Chemistry - A ...

https://en.wikipedia.org/wiki/María_Antonia_Herrero

"Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization". Nature Cell Biology. 9 (3): 276 ... Production of IL-1β downstream of caspase-11 requires another canonical inflammasome, called the NLRP3 inflammasome, that ... The CARD domain of caspase-11 has been shown to associate with AIP-1 and cofilin to facilitate actin depolymerization. In ... This mechanism contrasts to that of the canonical inflammasome, in which a bacterial ligand activates caspase-1 through an ...

https://en.wikipedia.org/wiki/Caspase_11

Toshima J, Toshima JY, Amano T, Yang N, Narumiya S, Mizuno K (2001). "Cofilin phosphorylation by protein kinase testicular ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of ... Dual specificity testis-specific protein kinase 1 is an enzyme that in humans is encoded by the TESK1 gene. This gene product ... "Entrez Gene: TESK1 testis-specific kinase 1". Toshima JY, Toshima J, Watanabe T, Mizuno K (November 2001). "Binding of 14-3- ...

https://en.wikipedia.org/wiki/TESK1

... cofilin 1 MeSH D12.776.220.525.212.750 - cofilin 2 MeSH D12.776.220.525.212.875 - destrin MeSH D12.776.220.525.246.500 - actin- ... neuregulin-1 See List of MeSH codes (D12.776.476). MeSH D12.776.503.280.249.500 - mannose-binding lectin MeSH D12.776.503.280. ... hiv-1 reverse transcriptase MeSH D12.776.964.970.880.325 - gene products, env MeSH D12.776.964.970.880.325.330 - hiv envelope ... neurofibromin 1 MeSH D12.776.402.150.500.500 - p120 gtpase activating protein MeSH D12.776.402.300.700.500 - ras-GRF1 MeSH ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D12.776)

... cofilin 2 MeSH D05.750.078.730.212.875 - destrin MeSH D05.750.078.730.246 - actin-related protein 2-3 complex MeSH D05.750. ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(D05)

The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of ... Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever ... Destrin binds actin and is thought to connect it as gelsolin segment-1 does. ...

https://en.wikipedia.org/wiki/Destrin

... like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans". Biomolecular NMR Assignments. 9 (2): 261-265. doi: ... Shukla, Vaibhav Kumar; Kabra, Ashish; Yadav, Rahul; Ono, Shoichiro; Kumar, Dinesh; Arora, Ashish (1 October 2015). "NMR ... 1 February 2017). "Unraveling the stereochemical and dynamic aspects of the catalytic site of bacterial peptidyl-tRNA hydrolase ...

https://en.wikipedia.org/wiki/Ashish_Arora

In accordance with their proposed rule, proteins like ADF, cofilin and LIMK1 are slingshot substrates. Phosphatases of ... Regenerating Liver (PRLs): Three PRL genes were described in mammals (PRL-1, PRL-2 and PRL-3). They share a high sequence ...

https://en.wikipedia.org/wiki/Dual-specificity_phosphatase

The latter is the enzyme that uses ATP to phosphorylate and inactivate the actin-depolymerizing factor cofilin. When cofilin is ... In the presence of Pyr1, LIMK1 is inhibited, which means that the phosphorylation of cofilin decreases, which results in the ... Pyr 1 is a cell permeable competitive inhibitor of Lim Kinase (especially LIMK1). ...

https://en.wikipedia.org/wiki/Pyr1

ADF/cofilin are the only substrates yet identified for the LIM kinases. LIM kinases directly phosphorylate and inactivate ... are actin-binding kinases that phosphorylate members of the ADF/cofilin family of actin binding and filament severing proteins ... LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal ... members of the cofilin family, resulting in stabilization of filamentous (F)-actin. Lim kinases are activated by signaling ...

https://en.wikipedia.org/wiki/Lim_kinase

Meanwhile, phosphorylation of PI3K by SRC activates RhoA which leads to activation of cofilin-P and disruption of filamentous ... When the WAVE-1 gene was disrupted in mice, it resulted in cognitive defects such as losses in learning and memory implicating ... 51 (1): 43-56. doi:10.1016/j.neuron.2006.05.018. PMC 2587166. PMID 16815331. Ohkubo, T; Ozawa, M. (1999). "p120ctn Binds to the ... 28 (1): 245-259. doi:10.1016/S0896-6273(00)00100-8. PMID 11086998. S2CID 17810792. Tanaka, H; Shan, W; Phillips, GR; Arndt, K; ...

https://en.wikipedia.org/wiki/Cadherin–catenin_complex_in_learning_and_memory

... which then stimulates cofilin, which effectively reorganizes the actin cytoskeleton of the cell. In the case of neurons, ... The LINGO-1 structure has been shown to be highly stable both in its crystal form and in solution, thanks to its leucine-rich ... LINGO-1 is coded by the LINGO-1 gene, which is located on the human chromosome 15, more precisely on the locus 15q24-26, which ... LINGO-1 is expressed almost exclusively in the central nervous system (CNS). It can be found in the brain and in neurons and ...

https://en.wikipedia.org/wiki/LINGO1

2005). "Ribosomal protein S18 identified as a cofilin-binding protein by using phage display library". Mol. Cell. Biochem. 262 ... 239 (1): 144-9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID 8706699. Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed ... 12 (1): 1-11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033. Mishra-Gorur K, Singer HA, Castellot JJ (2002 ... 1-2): 187-93. doi:10.1023/B:MCBI.0000038234.35936.1c. PMID 15532723. S2CID 30534195. Andersen JS, Lam YW, Leung AK, et al. ( ...

https://en.wikipedia.org/wiki/40S_ribosomal_protein_S18

1999). "Aip1p interacts with cofilin to disassemble actin filaments". J. Cell Biol. 145 (6): 1251-64. doi:10.1083/jcb.145.6. ... 327 (1): 268-75. doi:10.1016/j.bbrc.2004.11.156. PMID 15629458. Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a ... 56 (1): 59-69. doi:10.1006/geno.1998.5672. PMID 10036186. "Entrez Gene: WDR1 WD repeat domain 1". Dawson SJ, White LA (1992). " ... 23 (1): 94-101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157. Fujibuchi T, Abe Y, Takeuchi T, et al. (2005). "AIP1/WDR1 ...

https://en.wikipedia.org/wiki/WDR1

Existing strands can be interrupted by filament cleaving proteins, such as cofilin and gelsolin. Cofilin binds along two actin- ... They possess a number of accessory proteins including ADF/cofilin, which has a molecular weight of 16kDa and is coded for by a ... The import of actin into the nucleus (probably in a complex with cofilin) is facilitated by the import protein importin 9. Low ... Some proteins, such as cofilin appear to increase the angle of turn, but again this could be interpreted as the establishment ...

https://en.wikipedia.org/wiki/Actin

"Memo is a cofilin-interacting protein that influences PLCgamma1 and cofilin activities, and is essential for maintaining ... 78 (1-2): 46-54. doi:10.1006/geno.2001.6640. PMID 11707072. Qiu C, Lienhard S, Hynes NE, Badache A, Leahy DJ (February 2008). " ... "Entrez Gene: Mediator of cell motility 1". Meira M, Masson R, Stagljar I, Lienhard S, Maurer F, Boulay A, Hynes NE (March 2009 ... Mediator of cell motility 1 is a protein that in humans is encoded by the MEMO1 gene. GRCh38: Ensembl release 89: ...

https://en.wikipedia.org/wiki/MEMO1

Na⁺-K⁺ pump also interacts with ankyrin, IP3R, PI3K, PLC-gamma and cofilin. The Na⁺-K⁺ pump has been shown to control and set ... Lee K, Jung J, Kim M, Guidotti G (January 2001). "Interaction of the alpha subunit of Na,K-ATPase with cofilin". The ... 17 (1): 317-26. doi:10.1091/mbc.E05-08-0735. PMC 1345669. PMID 16267270. Li Z, Cai T, Tian J, Xie JX, Zhao X, Liu L, et al. ( ... 252 (3 Pt 1): C328-34. doi:10.1152/ajpcell.1987.252.3.c328. PMID 3030131. Glitsch HG, Tappe A (January 1993). "The Na+/K+ pump ...

https://en.wikipedia.org/wiki/Sodium–potassium_pump

Cai L, Marshall TW, Uetrecht AC, Schafer DA, Bear JE (May 2007). "Coronin 1B coordinates Arp2/3 complex and cofilin activities ... Whereas coronin 1 was required for chemokine-mediated migration, it was dispensable for T cell antigen receptor functions in T ... N-terminus signature region is reduced to 5aa and appears in front of each WD-repeat core domain (e.g., CRN7, POD-1) Human ... 14 (1): 87-96. doi:10.1016/j.str.2005.09.013. PMID 16407068. Li D, Roberts R (December 2001). "WD-repeat proteins: structure ...

https://en.wikipedia.org/wiki/Coronin

When the nascent endosome forms, the recruitment of cofilin by ClC-5 is a prerequisite for the localized dissolution of the ... Hryciw DH, Wang Y, Devuyst O, Pollock CA, Poronnik P, Guggino WB (October 2003). "Cofilin interacts with ClC-5 and regulates ... Hryciw DH, Wang Y, Devuyst O, Pollock CA, Poronnik P, Guggino WB (October 2003). "Cofilin interacts with ClC-5 and regulates ... It was demonstrated at the C-terminus of ClC-5 binds the actin-depolymerizing protein cofilin. ...

https://en.wikipedia.org/wiki/CLCN5

ADF/cofilins, which include ADF, cofilin, destrin, actophorin, coactosin, depactin and glia maturation factors (GMFs) beta and ... ADF/cofilins are small actin-binding proteins composed of a single ADF-H domain. They bind both actin-monomers and filaments ... ADF/cofilins bind ADP-actin with higher affinity than ATP-actin and inhibit the spontaneous nucleotide exchange on actin ... cofilin". J. Biol. Chem. 277 (45): 43089-95. doi:10.1074/jbc.M208225200. PMID 12207032. Liu LX, Xu H, Weller PF, Shi A, Debnath ...

https://en.wikipedia.org/wiki/ADF-H_domain

Spine heads are enlarged due to the increased phosphorylation of cofilin, leading to a decrease in F-actin severing and ... 321 (1): 210-8. doi:10.1016/j.bbrc.2004.06.122. PMID 15358237. Rumbaugh G, Adams JP, Kim JH, Huganir RL (March 2006). "SynGAP ... 1005 (1-2): 44-50. doi:10.1016/j.brainres.2004.01.032. PMID 15044063. S2CID 33854259. Brandenberger R, Wei H, Zhang S, Lei S, ... 21 (12): 1-13. doi:10.1038/s41593-018-0268-0. ISSN 1546-1726. PMC 6309426. PMID 30455457. Vlaskamp DR, Shaw BJ, Burgess R, Mei ...

https://en.wikipedia.org/wiki/SYNGAP1

Madak-Erdogan, Zeynep; Ventrella, Rosa; Petry, Luke; Katzenellenbogen, Benita S (2014). "Novel Roles for ERK5 and Cofilin as ... 92 (1): 125-34. doi:10.1210/endo-92-1-125. PMID 4681918. "People , Carl R. Woese Institute for Genomic Biology". www.igb. ... 31 (1): 226-36. doi:10.1128/MCB.00821-10. PMC 3019850. PMID 20956553. Harrington, William R; Kim, Sung Hoon; Funk, Cory C; ... 105 (1): 33-40. doi:10.1210/endo-105-1-33. PMID 446414. Gong, Ping; Madak-Erdogan, Zeynep; Li, Jilong; Cheng, Jianlin; ...

https://en.wikipedia.org/wiki/Benita_Katzenellenbogen

LIM-kinase will in turn phosphorylate and inactivate cofilin, which will prevent the breakdown and recycling of actin filaments ... 28 (1-2): 65-76. doi:10.1007/s10555-008-9170-7. PMID 19160018. S2CID 33869424. Kimura, K.; Ito, M.; Amano, M.; Chihara, K.; ... 6 (1): 21-30. doi:10.1038/ncb1075. PMC 2605950. PMID 14647292. Maul, R. S. (February 3, 2003). "EPLIN regulates actin dynamics ... 6 (1): 275-302. doi:10.1146/annurev.bioeng.6.040803.140040. PMID 15255771. Chen, Christopher S.; Tan, John; Tien, Joe (August ...

https://en.wikipedia.org/wiki/Stress_fiber

While the repulsive cue, Slit, is suggested to stimulate the translation of Cofilin (an actin depolymerizing factor) in growth ... If parameter k is decreased one-by-one from k=n through k=1 then more and more edges appear in the graph, since the inclusion ... However, as axons approach the midline, the repellent action of Slit is suppressed by Robo-3/Rig-1 receptor. Once the axons ... 11 (1): 113-116. arXiv:1602.04776. doi:10.1007/s11571-016-9407-z. PMC 5264751. PMID 28174617. Kerepesi, Csaba; Szalkai, Balazs ...

https://en.wikipedia.org/wiki/Axon_guidance

Freeman, S. A.; Lei, V; Dang-Lawson, M; Mizuno, K; Roskelley, C. D.; Gold, M. R. (2011). "Cofilin-mediated F-actin severing is ... 28 (1): 75-87. doi:10.1016/j.immuni.2007.11.019. PMID 18191594. McLeod, S. J.; Li, A. H.; Lee, R. L.; Burgess, A. E.; Gold, M. ... marginal zone and B-1 B cell function, and autoantibody responses". The Journal of Immunology. 183 (9): 5673-84. doi:10.4049/ ... R. (2002). "The Rap GTPases regulate B cell migration toward the chemokine stromal cell-derived factor-1 (CXCL12): Potential ...

https://en.wikipedia.org/wiki/Michael_R._Gold

Important roles are played by actin binding proteins like actin-binding Rho-activating protein (ABRA), cofilin, and thymosin ... 2009 Jan;104(1):5-21. Epub 2008 Dec 20. Hypoxia appears to initiate dilation by causing release of an as yet unknown and yet-to ... Pain was observed in all nine patients with 0 or 1 filling, but in only five of 14 patients with grade 2 or 3 filling. Thus, ... egr-1, carp, ets, by the Rho pathway and by the mitogen activated kinases ERK-1 and -2. In spite of the enormous increase in ...

https://en.wikipedia.org/wiki/Collateralization

Actin remodeling is generally modulated by the actin severing factor cofilin. Nef is able to associate with the cellular kinase ... HIV-1 Nef lowers the threshold for activation of CD4+ lymphocytes, but is not sufficient to cause activation in the absence of ... January 2004). "HIV-1 Nef Binds the DOCK2-ELMO1 Complex to Activate Rac and Inhibit Lymphocyte Chemotaxis". PLOS Biol. 2 (1): ... Since the activation state of the infected cell plays an important role in the success rate of HIV-1 infection, it is important ...

https://en.wikipedia.org/wiki/Nef_(protein)

Moriyama K, Yahara I (2003). "Human CAP1 is a key factor in the recycling of cofilin and actin for rapid actin turnover". J. ... 36 (1): 40-5. doi:10.1038/ng1285. PMID 14702039. Dodatko T, Fedorov AA, Grynberg M, et al. (2004). "Crystal structure of the ... 138 (1-2): 171-4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298. Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). " ... Adenylyl cyclase-associated protein 1 is an enzyme that in humans is encoded by the CAP1 gene. The protein encoded by this gene ...

https://en.wikipedia.org/wiki/CAP1

August 2009). "Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation ... including cofilin, fascin, Arg kinase, and mDia2. Invadopodia are considered mature when matrix metalloproteases (MMPs), ... 40 (1): 129-32. doi:10.1042/BST20110685. PMC 3425387. PMID 22260678. (Cell anatomy, Membrane biology). ...

https://en.wikipedia.org/wiki/Invadopodia

For example, profilin and cofilin are downregulated in knobs. Moreover, the transcription pattern of sticky knobs shows ... Ahren, D. (1 March 2005). "Comparison of gene expression in trap cells and vegetative hyphae of the nematophagous fungus ... 53 (1): 67-95. doi:10.1146/annurev-phyto-080614-120336. PMID 25938277. Zhao, Yong; Su, Hao; Zhou, Jing; Feng, Huihua; Zhang, Ke ... 51 (1): 89-113. Ke-Qin Zhang; Kevin D. Hyde, eds. (2014). Nematode-trapping fungi (Aufl. 2014 ed.). Dordrecht: Springer ...

https://en.wikipedia.org/wiki/Dactylellina_haptotyla

ADF/cofilins) VASP Vav Verprolin VDAC Vibrio cholerae RTX toxin Villin Vinculin Vitamin D-binding protein WIP WASp Y-box ... ADF/cofilin) Dystonins Diaphanous Dystroglycan DNase I Dystrophin Doliculide Dolastatins EAST Endossin EF-1a (ABP50) Eps15 EF- ... Adducin ADF/Cofilin Adseverin (scinderin) Afadin AFAP-110 Affixin Aginactin AIP1 Aldolase Angiogenin Anillin Annexins ... CapZ/Capping Protein a-Catenin Cofilin CR16 Caldesmon CCT Comitin Calicin Centuarin Coronin DBP40 Drebrin Dematin (Band 4.9) ...

https://en.wikipedia.org/wiki/Actin-binding_protein

2): A-region with a stretch of acidic residues: 32-45 AB-region, an actin monomer-binding region: 59-102 C-region, a cofilin ... central or cofilin homology) and A (acidic) region. The actin monomer-binding region of ActA has functional properties like the ... 60 (1): 219-30. doi:10.1128/iai.60.1.219-230.1992. PMC 257526. PMID 1309513. Domann E, Wehland J, Rohde M, et al. (May 1992). " ... 155 (1): 89-100. doi:10.1083/jcb.200106061. PMC 2150787. PMID 11581288. Bear J.E., Svitkina T.M., Krause M., et al. (May 2002 ...

https://en.wikipedia.org/wiki/Actin_assembly-inducing_protein

... which in turn phosphorylates ADF/cofilin, thereby inactivating its actin-depolymerization activity. This results in the ... 1. ROCK is a key regulator of actin organization and thus a regulator of cell migration as follows: Different substrates can be ... 7 (1): 12-23. doi:10.1016/S0960-9822(06)00023-6. PMID 8999996. S2CID 16144917. Kosako H, Yoshida T, Matsumura F, Ishizaki T, ... "Entrez Gene: PTEN phosphatase and tensin homolog (mutated in multiple advanced cancers 1)". Gao SY, Li CY, Chen J, Pan L, Saito ...

https://en.wikipedia.org/wiki/Rho-associated_protein_kinase

Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (August 2001). "Cofilin phosphorylation and actin reorganization activities ... 61 (1): 44-54. doi:10.1006/geno.1999.5922. PMID 10512679. "Entrez Gene: TESK2 testis-specific kinase 2". Kimura K, Wakamatsu A ... 16 (1): 55-65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560. Toshima JY, Toshima J, Watanabe T, Mizuno K (November 2001 ... v t e (Articles with short description, Short description matches Wikidata, Genes on human chromosome 1, All stub articles, ...

https://en.wikipedia.org/wiki/TESK2

Coactosin-like 1 antagonizes cofilin to promote lamellipodial protrusion at the immune synapse. ... Dive into the research topics of Coactosin-like 1 antagonizes cofilin to promote lamellipodial protrusion at the immune ...

https://mayoclinic.pure.elsevier.com/en/publications/coactosin-like-1-antagonizes-cofilin-to-promote-lamellipodial-pro/fingerprints/

In this study, we demonstrate the pre-symptomatic dysregulation of cytoskeleton assembly and its associated cofilin-1 pathway ... and upregulation of p-cofilin(Ser3). This upregulated p-cofilin(Ser3) showed redistribution of expression predominantly in the ... Saima Zafar 1 , Neelam Younas 2 , Nadeem Sheikh 3 , Waqas Tahir 2 , Mohsin Shafiq 2 , Matthias Schmitz 2 , Isidre Ferrer 4 5 , ... At pathological level, cofilin-1 expression was significantly altered in cortex and cerebellum in both humans and mice at pre- ...

https://pubmed.ncbi.nlm.nih.gov/28573459/

HIV-1 manipulates cofilin-1 regulation as a pre- and post-entry requisite. Disruption of ADF signaling is associated with ... cofilin-1 is a key cytoskeleton component that serves to lessen cortical actin. ... The mRNA expression levels for the ADF: (A) Cofilin-1; ABP: (B) Gelsolin; and IFN-IP: (C) PKR are shown. All data were ... B.2) Cofilin-1 will begin the breakdown of actin filaments and subsequently lead to viral fusion as the final entry requirement ...

http://www.ncbi.nlm.nih.gov/pubmed/36992512

Cofilin 1/metabolism; DNA/metabolism; Endothelial Cells/metabolism*; Gene Knockdown Techniques; Humans; Inflammation/metabolism ... MeSH Terms: Adherens Junctions/metabolism*; Autophagy/drug effects; Autophagy/genetics*; Beclin-1/genetics; Beclin-1/metabolism ...

https://tools.niehs.nih.gov/portfolio/index.cfm?do=portfolio.publicationDetail&id=3667453

Detection of sputum cofilin-1 as indicator of malignancy.. Rangel MP; Antonangelo L; Acencio MMP; Faria CS; de Sá VK; Leão PS; ... The role of cofilin-l in vulvar squamous cell carcinoma: A marker of carcinogenesis, progression and targeted therapy.. Wu Q; ...

https://pubmedhh.nlm.nih.gov/biomarkers/search.php?id=26936386&mod=related&page=1&outid=&proj=

recently identified 24 novel DMF-modified proteins in neurons and astrocytes, including cofilin-1, tubulin and collapsing ... 1: Targeting enzymes of glycolysis as an anti-inflammatory strategy.. Inhibition of hexokinase by 2-DG and GAPDH by Heptelidic ... Table 1 Table summarizing consequences of targeting immune cells with DMF, Metformin, Methotrexate and Rapamycin, listing cell ... IL-1β and TNFα suppress activity and expression levels of IDH in primary chondrocytes.198 Specific mutations in IDH1 have been ...

https://www.nature.com/articles/s41422-020-0291-z?error=cookies_not_supported

ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... FAQs for SSH2 Lysate (H00085464-T01). (Showing 1 - 1 of 1 FAQ).. * Can this product, SSH2 overexpressed cell lysate (H00085464- ... a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM] ...

https://www.novusbio.com/products/ssh2-lysate_h00085464-t01

N-Cofilin Non-Muscle Cofilin Registry Number. 0. Public MeSH Note. 2006; COFILIN 1 was indexed under MICROFILAMENT PROTEINS ... Cofilin 1 Preferred Term Term UI T629414. Date02/02/2005. LexicalTag NON. ThesaurusID NLM (2006). ... Cofilin 1 Preferred Concept UI. M0480172. Registry Number. 0. Scope Note. Cofilin 1 is a member of the cofilin family of ... Non-Muscle Cofilin Term UI T629416. Date02/02/2005. LexicalTag NON. ThesaurusID NLM (2006). ...

https://meshb.nlm.nih.gov/record/ui?ui=D051338

... cofilin, phospho-cofilin, and SRF. Comparable loading of protein is confirmed by GAPDH staining. ... Dephosphorylation of cofilin in Gsn−/− mice. Remodeling of the actin cytoskeleton is essential for cellular processes such as ... Dephosphorylation of cofilin in Gsn−/− mice. Western blot of protein extracts from hippocampus and frontal cortex of control ... 9). Dephosphorylation of cofilin in Gsn−/− mice was not related to changes of either SRF or actin protein levels. mRNA levels ...

https://www.jneurosci.org/content/30/9/3419.long

Colchicine reduces platelet aggregation by modulating cytoskeleton rearrangement via inhibition of cofilin and LIM domain ... 1 Institute of Chemical and Industrial Bioengineering, Jilin Engineering Normal University, Changchun, PR China. ... 1 Institute of Chemical and Industrial Bioengineering, Jilin Engineering Normal University, Changchun, PR China. ... kinase 1. Cimmino G, Tarallo R, Conte S, Morello A, Pellegrino G, Loffredo FS, Calì G, De Luca N, Golino P, Trimarco B, Cirillo ...

https://pubmed.ncbi.nlm.nih.gov/37026906/

Role of Cofilin in T Cell Function & HIV Pathogenesis ... Role of Cofilin in T Cell Function & HIV Pathogenesis. Your ... Cofilin is a member of the actin-depolymerizing factor (ADF)/cofilin family of proteins that bind and depolymerize filamentous ... Cofilin is also an essential player in T cell activation, during which cofilin is activated by co-stimulatory signaling, ... Role of Cofilin in T Cell Function & HIV Pathogenesis. Download VideoCast. You can download this VideoCast and play it on your ...

https://videocast.nih.gov/watch=33224

... the evolutionarily conserved leukocyte-specific motor protein Myo9B and the downstream cytoskeletal effector molecules Cofilin ... Kliniken & Institute … SFB / DZG SFB TRR156 Research 1. Funding Period Research Area B: Immune… ... governs F-actin turnover) and Fascin-1 (bundles F-actin, specifically expressed in DC) will be elucidated by using genetically ... will be assessed using mice deficient for Fascin-1 or for Myo9B in DC. ...

https://www.klinikum.uni-heidelberg.de/sfbs/sfb-trr156/research/research-area-b-immune-cross-talk-in-skin/b01

... in a complex with cofilin, which requires a functional G-actin-binding site on cofilin. Taurine chloramine (TnCl), a ... Incubate oxidized cofilin with methionine sulfoxide reductases leads to reduction of methionine sulfoxide back to Met, ... We found that TnCl inhibits recombinant human cofilin-1 to bind and depolymerize F-actin. DTT does not reactivate TnCl-treated ... Methionine as a redox switch regulating biological activities of cofilin. Monday, September 22, 2014 - Poster Session II ...

https://researchfestival.nih.gov/festival14/poster-MOLBIO-21.html

Cofilin, Non-Muscle. N-Cofilin. Non Muscle Cofilin. Non-Muscle Cofilin. Tree number(s):. D05.750.078.730.212.500. D12.776. ... Cofilin 1 - Preferred Concept UI. M0480172. Scope note. Cofilin 1 is a member of the cofilin family of proteins that is ... Cofilin 1 is a member of the cofilin family of proteins that is expressed in non-muscle CELLS. It has ACTIN depolymerization ... cofilina 1. Scope note:. Miembro de la familia de proteínas de la cofilina que se expresa en CÉLULAS que no son musculares. ...

https://decs.bvsalud.org/en/ths/resource/?id=50555

https://meshb-prev.nlm.nih.gov/record/ui?ui=D051338

... cofilin, peroredoxins, SH3L1, and others, showed sporadic presence and high expression ... 2 to 40 mu g L-1 (r(2) , 0 99), and detection limits were in t. Posted on May 28, 2021. by admin ... 2 to 40 mu g L-1 (r(2) , 0.99), and detection limits were in the range from 0.062 to 0.21 mu g L-1. Real water samples were ... Other proteins, such as psoriasin, galectin 1, cofilin, peroredox. Posted on May 29, 2021. by admin ...

https://bcl-2protein.com/2021/05

... cofilin-2. Am J Hum Genet. 2007 Jan. 80(1):162-7. [QxMD MEDLINE Link]. ... X-linked dominant scapuloperoneal myopathy is due to a mutation in the gene encoding four-and-a-half-LIM protein 1. Am J Hum ... Sato I, Wu S, Ibarra MC, Hayashi YK, Fujita H, Tojo M. Congenital neuromuscular disease with uniform type 1 fiber and RYR1 ... Four and a half LIM protein 1 gene mutations cause four distinct human myopathies: a comprehensive review of the clinical, ...

http://emedicine.medscape.com/article/1175852-overview

Cofilin-1 Maintains Prosurvival Signaling in Chronic Lymphocytic Leukemia Cells. Karp, Marta; Karczmarczyk, Agnieszka; Bojarska ... In healthy cells, cofilin-1 (CFL1) has multiple functions. Its role was described in several malignancies. The aim of this ... and cofilin-1 (CFL1), exposed on the surface of apoptotic cells have been identified as autoantigens that are recognized by the ... In conclusion, PD-1 might be an independent, universal marker of CLL cells and a part of their activated phenotype, and ...

https://pesquisa.bvsalud.org/controlecancer/?lang=pt&q=au:ZajAc,+MaLgorzata

Synonyms: p-cofilin (hSer 3)-R, sc-12912-r Antigens. Gene:. cfl1 ... epitope corresponding to phosphorylated Ser 3 of Cofilin of ...

https://test.xenbase.org/xenbase/reagents/antibodyAction.do?method=displayAntibodySummary&antibodyId=14579668

Stable for 1 year. The products are are safely shipped at ambient temperature for both domestic and international shipments. ... Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% ... Cells were then either left untreated (Cat.# JL9401) or treated with pervanadate (1 mM) for 30 minutes at 37°C (cat.# JL9501). ... Western blot analysis of A431, HeLa, and Jurkat cells (20 µg/lane) untreated or treated with pervanadate (1 mM) for 30 min. ...

https://ecmbio.com/collections/lysate/products/jl9501

N0000179563 Poloxamer 188 N0000167738 Cyclooxygenase 2 N0000169104 Neurofibromin 2 N0000169168 Galectin 2 N0000169452 Cofilin 2 ... 2-Ethyl-1,3,4,6,7,11b-hexahydro-3-isobutyl-9,10-dimethoxy- N0000175328 beta 2-Glycoprotein I N0000169400 beta 2-Microglobulin ... Member 1 N0000180212 Nuclear Receptor Subfamily 1, Group F, Member 1 N0000180275 Nuclear Receptor Subfamily 1, Group F, Member ... Member 1 N0000180286 Nuclear Receptor Subfamily 2, Group C, Member 2 N0000180203 Nuclear Receptor Subfamily 4, Group A, Member ...

https://evs.nci.nih.gov/ftp1/FDA/ndfrt/Archive/StructuralClass%202013-10-07.txt

GPCR-mediated PLCβγ/PKCβ/PKD signaling pathway regulates the cofilin phosphatase slingshot 2 in neutrophil chemotaxis. Mol Biol ... 2015 Mar 1;26(5):874-86.. Xu X, Meckel T, Brzostowski JA, Yan J, Meier-Schellersheim M, Jin T. Coupling mechanism of a GPCR and ... 2007 Jul 2;178(1):141-53.. Xu X, Meier-Schellersheim M, Jiao X, Nelson LE, Jin T. Quantitative imaging of single live cells ...

https://www.niaid.nih.gov/node/16880

pPAK and cofilin pathologies along with the loss of postsynaptic drebrin a protein localized at spines - suggested to Cole and ... Normal functioning of cofilin is essential for the maintenance of actin dynamics. Intracellular inclusion bodies containing ... PAK inhibition resulting in drebrin and cofilin pathologies and behavioral deficits may be Ab-induced. For example, in their ... led to cofilin pathology, significant drebrin loss and memory deficits like those observed in AD and Down syndrome. ...

https://grants.nih.gov/grants/guide/pa-files/PA-09-061.html

Cofilin 1-Mediated Biphasic F-Actin Dynamics of Neuronal Cells Affect Herpes Simplex Virus 1 Infection and Replication. Journal ... Figure 1 Single-cell RNA-sequencing identified MAMDC2-AS1 as a lncRNA associated with HSV-1 IE genes expression. (A) Heatmap of ... 1. Jiang YC, Feng H, Lin YC, Guo XR. New strategies against drug resistance to herpes simplex virus. Int J Oral Sci. 2016;8:1-6 ... 2002;1:13-25 4. Wang Y, Jin F, Wang R, Li F, Wu Y, Kitazato K. et al. HSP90: a promising broad-spectrum antiviral drug target. ...

https://www.ijbs.com/v16p1586.htm

Identification of a cofilin-like actin-binding site on translationally controlled tumor protein (TCTP). FEBS Lett, 584(23):4756 ... A common cofilin activity cycle in invasive tumor cells and inflammatory cells. J Cell Sci, 122(3):305-311. ... Poly(ADP-ribose) glycohydrolase silencing down-regulates TCTP and Cofilin-1 associated with metastasis in benzo(a)pyrene ... 1. MicroRNAs in breast cancer: oncogene..... 2. Verticillin A inhibits colon cancer..... 3. Use of liposomal doxorubicin for ...

https://jzus.zju.edu.cn/article.php?doi=10.1631/jzus.B2100910

... cofilin 1;; cMDH1; cytosolic NAD-dependent malate dehydrogenase 1; CTSK; cathepsin K; Cu-Zn SOD; Cu/Zn superoxide dismutase; ... بیان اندوتلین-1 در لوسمی لنفوسیتی مزمن با مکانیزم های اپی ژنتیک و محرک های خارج سلولی کنترل می شود ... Keywords: گلیسرولیدید 3-فسفات دهیدروژناز; Thymic stromal lymphopoietin; Cysteine; Human mast cell; Caspase-1; Nuclear factor- ... Keywords: گلیسرولیدید 3-فسفات دهیدروژناز; Whey protein; In vitro digestion; Bioactive peptide; Glucagon-like peptide-1; ...

https://daneshyari.com/isi/articles/glyceraldehyde_3-phosphate_dehydrogenase

In the present study, we examined the cofilin expression in human ICH autopsy brains. Then, the spatiotemporal cofilin ... Cofilin plays a crucial role in stress conditions, but its signaling following ICH in a longitudinal study is yet to be ... Cofilin plays a crucial role in stress conditions, but its signaling following ICH in a longitudinal study is yet to be ... Cofilin protein expression increased in the ipsilateral striatum on days 1 and 3 and then decreased from days 7 to 28. An ...

https://www2.mdpi.com/journal/cells/special_issues/Therapeutic_Nervous

... promotes cofilin-induced actin dynamics at pollen tube growth. November 2019, Dhaka, Bangladesh. BDNF signaling in local ... L:D cycle in a total of 32 samples with 4 replicates per treatment (1 per day and limited our interpretation to the antioxidant ... Our data are within the annotated transcriptome and SNSs with 2 mM K2HPO4 were diluted 1:100 into fresh MOPS-GLC medium. To ... B) Scores (based on canonical dimension 1, more irradiation-like gene expression response to live bacteria where to buy valtrex ...

http://kirkwoodrealestate.net/valtrex-online-pharmacy/

AN - see SCR for organism-specific terms HN - 2006(2005) MH - Cofilin 2 UI - D051340 MN - D5.750.78.730.212.750 MN - D12.776. ... HN - 2006(1981) BX - Cofilins MH - Actin-Related Protein 2 UI - D051377 MN - D5.750.78.730.246.500 MN - D12.776.220.525.246.500 ... Cofilin 1 is a member of the cofilin family of proteins that is expressed in non-muscle CELLS. It has ACTIN depolymerization ... 210.500.242 MN - D12.776.220.525.212.750 MS - A member of the cofilin family of proteins that is expressed in MUSCLE CELLS. It ...

https://nlmpubs.nlm.nih.gov/projects/mesh/.newterms/.bkup/mshnew2006.10-03-2005.txt

6.07 38542.03 10 8393101 cofilin-1 Cfl1 166 Rattus norvegicus ... AMP-activated protein kinase subunit gamma-1 Prkag1 330 Rattus ... 12 56090642 type-1 angiotensin II receptor-associated protein Agtrap 160 Rattus norvegicus ... 12 54400736 LETM1 and EF-hand domain-containing protein 1, mitochondrial precursor Letm1 739 Rattus norvegicus ... 12 56090283 RING finger and CHY zinc finger domain-containing protein 1 Rchy1 261 Rattus norvegicus ...

https://esbl.nhlbi.nih.gov/helixweb/Database/IMCD_Proteome/images/IMCD_Proteome.txt

Cofilin 1 is a member of the cofilin family of proteins that is expressed in non-muscle CELLS. (bvsalud.org)
Two key proteins for cellular communication between astrocytes and neurons are αvβ3 integrin and the receptor Thy-1. (abberior.rocks)
Examination of the protein-protein conversation between LpnE and a PKR Inhibitor eukaryotic protein obscurin-like protein 1 suggested that LpnE can interact with eukaryotic proteins made up of immunoglobulin-like folds via the SLR regions. (achemmic.com)
Sel1 and its homologues are involved in cell-to-cell relationships that designate the fate of cells during development through binding of the membrane proteins Lin-12 and Glp-1 (29). (achemmic.com)
These data suggest that the actin-regulating GNA13-mediated RHOA-ROCK-LIMK-cofilin pathway is present in bovine spermatozoa, that RHOA interacts with proteins involved in capacitation and the acrosome reaction, and that RHOA signaling in sperm may be targeted by AKAPs. (elsevierpure.com)

Short-interfering RNA downregulation of active form of cofilin-1 resulted in the redistribution/downregulation of PrP C , increase of activated form of microglia, accumulation of dense form of F-actin, and upregulation of p-cofilin(Ser3). (nih.gov)
In particular, the functional roles of the evolutionarily conserved leukocyte-specific motor protein Myo9B and the downstream cytoskeletal effector molecules Cofilin (governs F-actin turnover) and Fascin-1 (bundles F-actin, specifically expressed in DC) will be elucidated by using genetically modified DC. (uni-heidelberg.de)
The total amount of phosphorylated and dephosphorylated cofilin is certainly in turn one of the key modulators of actin filament assembly and disassembly. (bioshockinfinitereleasedate.com)
Binding of these molecules in the same (cis) or on adjacent (trans) cellular membranes induces Thy-1 clustering, triggering actin cytoskeleton remodeling. (abberior.rocks)
In conclusion, these data support the hypothesis that the Thy-1-CBP-Csk-Src-RhoA-ROCK axis transmitted signals from astrocytic integrin-engaged Thy-1 (trans) to the neuronal actin cytoskeleton. (abberior.rocks)
In somatic cells, RHOA mediates actin dynamics through a GNA13-mediated signaling cascade involving RHO kinase (ROCK), LIM kinase (LIMK), and cofilin. (elsevierpure.com)

In this study, we demonstrate the pre-symptomatic dysregulation of cytoskeleton assembly and its associated cofilin-1 pathway in strain and brain region-specific manners in MM1 and VV2 subtype-specific Creutzfeldt-Jakob disease at clinical and pre-clinical stage. (nih.gov)
In conclusion, our findings demonstrated for the first time a key pre-clinical response of cofilin-1 and the associated pathway in prion disease. (nih.gov)
This is the first study to detail the signaling pathway triggered by αvβ3, the endogenous Thy-1 ligand, highlighting the role of membrane-bound integrins as trans acting ligands in astrocyte-neuron communication. (abberior.rocks)

LIM kinase isoform 1 (LIMK1), slingshot phosphatase isoform 1 (SSH1), RhoA-associated kinase (Rock2), and amyloid precursor protein (APP) in sporadic Creutzfeldt-Jakob disease MM1 mice and in human MM1 and VV2 frontal cortex and cerebellum samples. (nih.gov)
This occurred concomitantly with inactivation and exclusion of the non-receptor tyrosine kinase Src from the Thy-1/C-terminal Src kinase (Csk)-binding protein (CBP)/Csk complex. (abberior.rocks)

This upregulated p-cofilin(Ser3) showed redistribution of expression predominantly in the activated form of microglia in PCNs. (nih.gov)
A significant function from the LIMKs may be the control of cofilin, which is inactivated by phosphorylation at Ser3. (bioshockinfinitereleasedate.com)

The Src inactivation decreased the p190Rho GTPase activating protein phosphorylation, promoting RhoA activation, cofilin, and myosin light chain II phosphorylation and, consequently, neurite shortening. (abberior.rocks)

This dependence on glycolysis is seen in classically activated M1 macrophages, monocytes, educated NK cells, activated B cells and effector T cells including T-helper (Th) 1 and Th17 cells where glycolysis is required for proper differentiation and immune effector functions. (nature.com)

1 Pro-inflammatory signals will induce a metabolic switch in both myeloid and lymphoid cells, resulting in upregulation of aerobic glycolysis in a manner similar to the Warburg effect described in cancer cells. (nature.com)
Cells were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 10 mM Tris (pH 7.4) buffer. (ecmbio.com)
Western blot analysis of A431, HeLa, and Jurkat cells (20 µg/lane) untreated or treated with pervanadate (1 mM) for 30 min. (ecmbio.com)
Herpes simplex virus (HSV) type 1 (HSV-1) infection exhibited high heterogeneity at individual cells level, including the different gene expression patterns and varying amounts of progeny virus. (ijbs.com)
MAMDC2-AS1 silencing reduces the expression of HSV-1 immediate early (IE) genes and limit HSV-1 infection in human host cells. (ijbs.com)
Our study elucidates the contribution of lncRNA to HSV-1 infection susceptibility in human cells and the role of Hsp90α RNA binding activity in HSV-1 infection. (ijbs.com)
IE genes (mainly include α0 , α4 , α22 , α27 , and α47 ) are the first set of HSV-1 genes to be transcribed and expressed in productively infected cells. (ijbs.com)

The MAMDC2-AS1-Hsp90α interaction facilitates the nuclear transport of viral tegument protein VP16, the core factor initiating the expression of HSV-1 IE genes. (ijbs.com)
Molecular events that could explain how the Thy-1-αvβ3 integrin interaction signals have only been studied separately in different cell types, and the detailed transcellular communication and signal transduction pathways involved in neuronal cytoskeleton remodeling remain unresolved. (abberior.rocks)

15(1): 75, 2023 05 03. (bvsalud.org)

SSH1: protein phosphatase Slingshot homolog 1. (bioshockinfinitereleasedate.com)

Consistently, ectopic expression of MAMDC2-AS1 enhances HSV-1 IE genes transcription and facilitates the formation of HSV-1-induced plaques. (ijbs.com)

Inactivation occurs via phosphatases In the meantime, such as for example slingshot 1 and chronophin (Scott and Olson, 2007). (bioshockinfinitereleasedate.com)

HSV-1 exhibit a strictly regulated temporal cascade of gene expression that can be divided into three general stage: immediate-early (IE), early (E) and late (L) [ 5 , 12 , 13 ]. (ijbs.com)
Finally, silencing the adaptor CBP demonstrated that this protein was a key transducer in the Thy-1 signaling cascade. (abberior.rocks)

Mechanically, both RNA-pull down and RNA immunoprecipitation assays show that MAMDC2-AS1 interacts with the RNA binding protein heat shock protein 90α (Hsp90α), a molecular chaperone involving in the nuclear import of HSV-1. (ijbs.com)

At pathological level, cofilin-1 expression was significantly altered in cortex and cerebellum in both humans and mice at pre-clinical stage and at early symptomatic clinical stage of the disease. (nih.gov)

The course of distinct cutaneous disease models (leishmaniasis, psoriasis, hapten- and protein-specific contact dermatitis and tolerance induction) will be assessed using mice deficient for Fascin-1 or for Myo9B in DC. (uni-heidelberg.de)
The transcription factor YY1 mediates the induction of MAMDC2-AS1 upon HSV-1 infection. (ijbs.com)

Description: A sandwich quantitative ELISA assay kit for detection of Canine Transforming Growth Factor Beta 1 (TGFb1) in samples from serum, platelet-poor plasma, tissue homogenates, cell culture supernates or other biological fluids. (bestpractice-life.pl)

Herpes simplex virus (HSV) type 1 (HSV-1) infection poses a major challenge to global health [ 1 , 2 ]. (ijbs.com)

Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). (ecmbio.com)
Mol Cell Biochem, 399 (1-2). (sciencecentral.in)
of an mutant of 130b in both the A549 and THP-1 cell lines. (achemmic.com)

Xu X, Gera N, Li H, Yun M, Zhang L, Wang Y, Wang QJ, Jin T. GPCR-mediated PLCβγ/PKCβ/PKD signaling pathway regulates the cofilin phosphatase slingshot 2 in neutrophil chemotaxis . (nih.gov)

Cofilin is a member of the actin-depolymerizing factor (ADF)/cofilin family of proteins that bind and depolymerize filamentous actin (F-actin) to regulate actin dynamics. (nih.gov)
We found that TnCl inhibits recombinant human cofilin-1 to bind and depolymerize F-actin. (nih.gov)

Cofilin 1 is a member of the cofilin family of proteins that is expressed in non-muscle CELLS . (nih.gov)

In human T cells, cofilin regulates T cell motility for T cell migration and homing to lymphoid and non-lymphoid tissues. (nih.gov)
The findings identify Met-115 as a redox switch on cofilin which regulates its biological activities. (nih.gov)
HN - 2006(1998) MH - Activating Transcription Factor 1 UI - D051697 MN - D12.776.260.108.61.500 MN - D12.776.930.127.61.500 MS - An activating transcription factor that regulates expression of a variety of genes including C-JUN GENES and TRANSFORMING GROWTH FACTOR BETA2. (nih.gov)

cofilin activity is required for cortical actin polymerization to stabilize immunological synapse between T cells and antigen presenting cells. (nih.gov)
Cofilin activity is frequently targeted by intracellular pathogens for actin-dependent intracellular migration. (nih.gov)
Our previous studies have shown that in HIV latent infection of blood CD4 T cells, the virus utilizes the chemokine receptor, CXCR4 or CCR5, to triggers cofilin activity, which promotes cortical actin dynamics to facilitate viral entry and intracellular migration (Yoder et al. (nih.gov)
His early study demonstrated a critical role of CXCR4/G-protein signaling in mediating cofilin activation and actin dynamics necessary for HIV nuclear migration and the infection of resting CD4 T cells (Cell, 2008,134:782). (nih.gov)
Currently, his lab is continuously studying the role of chemokine- and HIV-1-mediated G-protein signaling and actin dynamics in T cells. (nih.gov)
It accelerates F-actin depolymerization by promoting dissociation of monomeric globular-actin (G-actin) in a complex with cofilin, which requires a functional G-actin-binding site on cofilin. (nih.gov)
This residue was identified as Met-115, located in the G-actin-binding pocket, implying that oxidation of Met-115 disrupts the G-actin-binding site of cofilin and causes inactivation. (nih.gov)
HN - 2006(1981) BX - Cofilins MH - Actin-Related Protein 2 UI - D051377 MN - D5.750.78.730.246.500 MN - D12.776.220.525.246.500 MS - A PROFILIN binding domain protein that is part of the Arp2-3 complex. (nih.gov)

Phosphorylation is well-known to regulate cofilin activity. (nih.gov)

Our preliminary study using 2D isolates were recovered from liq- new cases throughout the world cu- gel electrophoresis in drug sensitive/ uid nitrogen, and sub-cultured in taneous leishmaniasis (CL) remains resistant strains of L. tropica showed RPMI1640 medium (Gibco/BRL) a serious public health problem in that some proteins were differentially supplemented with 10% fetal bovine numerous countries [1,2]. (who.int)

Recently, he also studied the pathogenic effects of HIV-mediated G protein signaling in HIV patients, and discovered chronic hyperactivation of cofilin in the blood resting CD4 T cells of HIV patients (Science Advances, 2019, 5:eaat7911). (nih.gov)

Recently, we further demonstrated that HIV-mediated cofilin activity has pathogenic consequences in triggering chronic cofilin hyperactivation in the blood resting CD4 T cells of HIV patients. (nih.gov)
In this seminar, I summarize my over 15 years of research on HIV exploiting the cofilin pathway in T cells. (nih.gov)

Taurine chloramine (TnCl), a physiological oxidant generated during inflammation, oxidizes Cys and Met on cofilin, but the effect of oxidation on its biological activity has not been studied. (nih.gov)
use ACYLTRANSFERASES 1973-1979, use COENZYME A & PHOSPHOLIPIDS 1973-1978 MH - 1-Pyrroline-5-Carboxylate Dehydrogenase UI - D050842 MN - D8.811.682.662.693 MS - An enzyme that catalyzes the oxidation of 1-pyrroline-5-carboxylate to L-GLUTAMATE in the presence of NAD. (nih.gov)

With HIV-1 Latency Reversal Activity. (nih.gov)

1 Chemotaxis Signaling Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD 20852. (nih.gov)

Cofilin is also an essential player in T cell activation, during which cofilin is activated by co-stimulatory signaling, including signaling from CD28/B7 and integrins/ligand interactions (e.g. (nih.gov)

This recent study also suggests that cofilin in a key molecule that may need to be therapeutically targeted for immune reconstitution to restore T cell motility. (nih.gov)

Table 1. (medscape.com)

Anatomy9

Chemicals and Drugs36

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes8

Disciplines and Occupations1

Technology, Industry, Agriculture1

Information Science2

The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics. (1/145)

The human D2 dopamine receptor synergizes with the A2A adenosine receptor to stimulate adenylyl cyclase in PC12 cells. (2/145)

Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. (3/145)

Maternal diet programs embryonic kidney gene expression. (4/145)

Micropuncture gene delivery and intravital two-photon visualization of protein expression in rat kidney. (5/145)

Ligation of cell surface-associated glucose-regulated protein 78 by receptor-recognized forms of alpha 2-macroglobulin: activation of p21-activated protein kinase-2-dependent signaling in murine peritoneal macrophages. (6/145)

Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C. (7/145)

Stimulation of actin polymerization by filament severing. (8/145)

Cofilin 1

Actin Depolymerizing Factors

Cofilin 2

Lim Kinases

Destrin

Microfilament Proteins

Actins

Actin Cytoskeleton

Pseudopodia

Phosphorylation

Profilins

Gelsolin

p21-Activated Kinases

Cell Surface Extensions

Actin-Related Protein 2-3 Complex

Phalloidine

Cytoskeleton

Ethenoadenosine Triphosphate

rho-Associated Kinases

Actin-Related Protein 3

Cell Movement

Thiazolidines

Biopolymers

Actin-Related Protein 2

Phosphoprotein Phosphatases

Nerve Tissue Proteins

Polymerization

CapZ Actin Capping Protein

Protein Kinases

rho GTP-Binding Proteins

Dendritic Spines

Contractile Proteins

Protein Binding

RNA, Small Interfering

Cytoskeletal Proteins

Actin Capping Proteins

Bicyclo Compounds, Heterocyclic

Phosphoric Monoester Hydrolases

Stress Fibers

Phosphatidylinositol 4,5-Diphosphate

14-3-3 Proteins

Molecular Sequence Data

Amino Acid Sequence

Protein Footprinting

Glia Maturation Factor

Binding Sites

Growth Cones

Organelle Shape

Protein Transport

Cell Membrane Structures

Enzyme-Linked Immunosorbent Assay

Transforming Growth Factor beta

Biological Science Disciplines

Transforming Growth Factor beta1

Receptors, Transforming Growth Factor beta

Platelet-Rich Plasma

Reagent Kits, Diagnostic

The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics. (1/145)

The human D2 dopamine receptor synergizes with the A2A adenosine receptor to stimulate adenylyl cyclase in PC12 cells. (2/145)

Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. (3/145)

Maternal diet programs embryonic kidney gene expression. (4/145)

Micropuncture gene delivery and intravital two-photon visualization of protein expression in rat kidney. (5/145)

Ligation of cell surface-associated glucose-regulated protein 78 by receptor-recognized forms of alpha 2-macroglobulin: activation of p21-activated protein kinase-2-dependent signaling in murine peritoneal macrophages. (6/145)

Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C. (7/145)

Stimulation of actin polymerization by filament severing. (8/145)

Cofilin 1

Cofilin-2

HSPH1

Reelin

Cyclase-associated protein family

SSH2

LIMK1

Alpha-actinin-4

LIMK2

SSH3

SSH1

Anne Bertolotti

PAK4

YWHAZ

María Antonia Herrero