Cofilin 1 is a member of the cofilin family of proteins that is expressed in non-muscle CELLS. It has ACTIN depolymerization activity that is dependent on HYDROGEN-ION CONCENTRATION.
A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. They remodel the actin CYTOSKELETON by severing ACTIN FILAMENTS and increasing the rate of monomer dissociation.
A member of the cofilin family of proteins that is expressed in MUSCLE CELLS. It has ACTIN depolymerization activity that is dependent on HYDROGEN-ION CONCENTRATION.
Serine protein kinases involved in the regulation of ACTIN polymerization and MICROTUBULE disassembly. Their activity is regulated by phosphorylation of a threonine residue within the activation loop by intracellular signaling kinases such as P21-ACTIVATED KINASES and by RHO KINASE.
A member of the actin depolymerizing factors. Its depolymerizing activity is independent of HYDROGEN-ION CONCENTRATION.
Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.
A dynamic actin-rich extension of the surface of an animal cell used for locomotion or prehension of food.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.
A family of low molecular weight proteins that bind ACTIN and control actin polymerization. They are found in eukaryotes and are ubiquitously expressed.
A 90-kDa protein produced by macrophages that severs ACTIN filaments and forms a cap on the newly exposed filament end. Gelsolin is activated by CALCIUM ions and participates in the assembly and disassembly of actin, thereby increasing the motility of some CELLS.
A family of serine-threonine kinases that bind to and are activated by MONOMERIC GTP-BINDING PROTEINS such as RAC GTP-BINDING PROTEINS and CDC42 GTP-BINDING PROTEIN. They are intracellular signaling kinases that play a role the regulation of cytoskeletal organization.
Specialized structures of the cell that extend the cell membrane and project out from the cell surface.
A complex of seven proteins including ARP2 PROTEIN and ARP3 PROTEIN that plays an essential role in maintenance and assembly of the CYTOSKELETON. Arp2-3 complex binds WASP PROTEIN and existing ACTIN FILAMENTS, and it nucleates the formation of new branch point filaments.
Very toxic polypeptide isolated mainly from AMANITA phalloides (Agaricaceae) or death cup; causes fatal liver, kidney and CNS damage in mushroom poisoning; used in the study of liver damage.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
1,N-6-Ethenoadenosine triphosphate. A fluorescent analog of adenosine triphosphate.
A group of intracellular-signaling serine threonine kinases that bind to RHO GTP-BINDING PROTEINS. They were originally found to mediate the effects of rhoA GTP-BINDING PROTEIN on the formation of STRESS FIBERS and FOCAL ADHESIONS. Rho-associated kinases have specificity for a variety of substrates including MYOSIN-LIGHT-CHAIN PHOSPHATASE and LIM KINASES.
A component of the Arp2-3 complex that is related in sequence and structure to ACTIN and that binds ATP. It is expressed at higher levels than ARP2 PROTEIN and does not contain a PROFILIN binding domain.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Reduced (protonated) form of THIAZOLES. They can be oxidized to THIAZOLIDINEDIONES.
Polymers synthesized by living organisms. They play a role in the formation of macromolecular structures and are synthesized via the covalent linkage of biological molecules, especially AMINO ACIDS; NUCLEOTIDES; and CARBOHYDRATES.
A PROFILIN binding domain protein that is part of the Arp2-3 complex. It is related in sequence and structure to ACTIN and binds ATP.
A group of enzymes removing the SERINE- or THREONINE-bound phosphate groups from a wide range of phosphoproteins, including a number of enzymes which have been phosphorylated under the action of a kinase. (Enzyme Nomenclature, 1992)
Chemical reaction in which monomeric components are combined to form POLYMERS (e.g., POLYMETHYLMETHACRYLATE).
An actin capping protein that binds to the barbed-ends of ACTIN filaments. It is a heterodimer consisting of an alpha and a beta subunit. It regulates actin assembly by stabilizing actin oligomers for elongation. In SKELETAL MUSCLE, CapZ is localized to the Z-disk.
A family of enzymes that catalyze the conversion of ATP and a protein to ADP and a phosphoprotein.
A large family of MONOMERIC GTP-BINDING PROTEINS that are involved in regulation of actin organization, gene expression and cell cycle progression. This enzyme was formerly listed as EC 3.6.1.47.
Spiny processes on DENDRITES, each of which receives excitatory input from one nerve ending (NERVE ENDINGS). They are commonly found on PURKINJE CELLS and PYRAMIDAL CELLS.
Proteins which participate in contractile processes. They include MUSCLE PROTEINS as well as those found in other cells and tissues. In the latter, these proteins participate in localized contractile events in the cytoplasm, in motile activity, and in cell aggregation phenomena.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Major constituent of the cytoskeleton found in the cytoplasm of eukaryotic cells. They form a flexible framework for the cell, provide attachment points for organelles and formed bodies, and make communication between parts of the cell possible.
Actin capping proteins are cytoskeletal proteins that bind to the ends of ACTIN FILAMENTS to regulate actin polymerization.
A class of saturated compounds consisting of two rings only, having two or more atoms in common, containing at least one hetero atom, and that take the name of an open chain hydrocarbon containing the same total number of atoms. (From Riguady et al., Nomenclature of Organic Chemistry, 1979, p31)
A group of hydrolases which catalyze the hydrolysis of monophosphoric esters with the production of one mole of orthophosphate. EC 3.1.3.
Bundles of actin filaments (ACTIN CYTOSKELETON) and myosin-II that span across the cell attaching to the cell membrane at FOCAL ADHESIONS and to the network of INTERMEDIATE FILAMENTS that surrounds the nucleus.
A phosphoinositide present in all eukaryotic cells, particularly in the plasma membrane. It is the major substrate for receptor-stimulated phosphoinositidase C, with the consequent formation of inositol 1,4,5-triphosphate and diacylglycerol, and probably also for receptor-stimulated inositol phospholipid 3-kinase. (Kendrew, The Encyclopedia of Molecular Biology, 1994)
A large family of signal-transducing adaptor proteins present in wide variety of eukaryotes. They are PHOSPHOSERINE and PHOSPHOTHREONINE binding proteins involved in important cellular processes including SIGNAL TRANSDUCTION; CELL CYCLE control; APOPTOSIS; and cellular stress responses. 14-3-3 proteins function by interacting with other signal-transducing proteins and effecting changes in their enzymatic activity and subcellular localization. The name 14-3-3 derives from numerical designations used in the original fractionation patterns of the proteins.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A method for determining points of contact between interacting proteins or binding sites of proteins to nucleic acids. Protein footprinting utilizes a protein cutting reagent or protease. Protein cleavage is inhibited where the proteins, or nucleic acids and protein, contact each other. After completion of the cutting reaction, the remaining peptide fragments are analyzed by electrophoresis.
A factor identified in the brain that influences the growth and differentiation of NEURONS and NEUROGLIA. Glia maturation factor beta is the 17-kDa polypeptide product of the GMFB gene and is the principal component of GLIA MATURATION FACTOR.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Bulbous enlargement of the growing tip of nerve axons and dendrites. They are crucial to neuronal development because of their pathfinding ability and their role in synaptogenesis.
The quality of surface form or outline of ORGANELLES.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
Structures which are part of the CELL MEMBRANE or have cell membrane as a major part of their structure.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.

The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics. (1/145)

Actin-depolymerizing factor (ADF)/cofilins are essential regulators of actin filament turnover. Several ADF/cofilin isoforms are found in multicellular organisms, but their biological differences have remained unclear. Herein, we show that three ADF/cofilins exist in mouse and most likely in all other mammalian species. Northern blot and in situ hybridization analyses demonstrate that cofilin-1 is expressed in most cell types of embryos and adult mice. Cofilin-2 is expressed in muscle cells and ADF is restricted to epithelia and endothelia. Although the three mouse ADF/cofilins do not show actin isoform specificity, they all depolymerize platelet actin filaments more efficiently than muscle actin. Furthermore, these ADF/cofilins are biochemically different. The epithelial-specific ADF is the most efficient in turning over actin filaments and promotes a stronger pH-dependent actin filament disassembly than the two other isoforms. The muscle-specific cofilin-2 has a weaker actin filament depolymerization activity and displays a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1 and ADF. In steady-state assays, cofilin-2 also promotes filament assembly rather than disassembly. Taken together, these data suggest that the three biochemically distinct mammalian ADF/cofilin isoforms evolved to fulfill specific requirements for actin filament dynamics in different cell types.  (+info)

The human D2 dopamine receptor synergizes with the A2A adenosine receptor to stimulate adenylyl cyclase in PC12 cells. (2/145)

The adenosine A(2A) receptor and the dopamine D(2) receptor are prototypically coupled to G(s) and G(i)/G(o), respectively. In striatal intermediate spiny neurons, these receptors are colocalized in dendritic spines and act as mutual antagonists. This antagonism has been proposed to occur at the level of the receptors or of receptor-G protein coupling. We tested this model in PC12 cells which endogenously express A(2A) receptors. The human D(2) receptor was introduced into PC12 cells by stable transfection. A(2A)-agonist-mediated inhibition of D(2) agonist binding was absent in PC12 cell membranes but present in HEK293 cells transfected as a control. However, in the resulting PC12 cell lines, the action of the D(2) agonist quinpirole depended on the expression level of the D(2) receptor: at low and high receptor levels, the A(2A)-agonist-induced elevation of cAMP was enhanced and inhibited, respectively. Forskolin-stimulated cAMP formation was invariably inhibited by quinpirole. The effects of quinpirole were abolished by pretreatment with pertussis toxin. A(2A)-receptor-mediated cAMP formation was inhibited by other G(i)/G(o)-coupled receptors that were either endogenously present (P(2y12)-like receptor for ADP) or stably expressed after transfection (A(1) adenosine, metabotropic glutamate receptor-7A). Similarly, voltage activated Ca(2+) channels were inhibited by the endogenous P(2Y) receptor and by the heterologously expressed A(1) receptor but not by the D(2) receptor. These data indicate functional segregation of signaling components. Our observations are thus compatible with the proposed model that D(2) and A(2A) receptors are closely associated, but they highlight the fact that this interaction can also support synergism.  (+info)

Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. (3/145)

Actin-depolymerizing factor (ADF)/cofilins are small actin-binding proteins found in all eukaryotes. In vitro, ADF/cofilins promote actin dynamics by depolymerizing and severing actin filaments. However, whether ADF/cofilins contribute to actin dynamics in cells by disassembling "old" actin filaments or by promoting actin filament assembly through their severing activity is a matter of controversy. Analysis of mammalian ADF/cofilins is further complicated by the presence of multiple isoforms, which may contribute to actin dynamics by different mechanisms. We show that two isoforms, ADF and cofilin-1, are expressed in mouse NIH 3T3, B16F1, and Neuro 2A cells. Depleting cofilin-1 and/or ADF by siRNA leads to an accumulation of F-actin and to an increase in cell size. Cofilin-1 and ADF seem to play overlapping roles in cells, because the knockdown phenotype of either protein could be rescued by overexpression of the other one. Cofilin-1 and ADF knockdown cells also had defects in cell motility and cytokinesis, and these defects were most pronounced when both ADF and cofilin-1 were depleted. Fluorescence recovery after photobleaching analysis and studies with an actin monomer-sequestering drug, latrunculin-A, demonstrated that these phenotypes arose from diminished actin filament depolymerization rates. These data suggest that mammalian ADF and cofilin-1 promote cytoskeletal dynamics by depolymerizing actin filaments and that this activity is critical for several processes such as cytokinesis and cell motility.  (+info)

Maternal diet programs embryonic kidney gene expression. (4/145)

Human epidemiological data associating birth weight with adult disease suggest that organogenesis is "programmed" by maternal diet. In rats, protein restriction in pregnancy produces offspring with fewer renal glomeruli and higher systemic blood pressures than controls. We tested the hypothesis that maternal diet alters gene expression in the metanephros, the precursor of the definitive mammalian kidney. We demonstrated that maternal low-protein diet initiated when pregnancy starts and maintained to embryonic day 13, when the metanephros consists of mesenchyme surrounding a once-branched ureteric bud, is sufficient to significantly reduce glomerular numbers in offspring by about 20%. As assessed by representational difference analyses and real-time quantitative polymerase chain reactions, low-protein diet modulated gene expression in embryonic day 13 metanephroi. In particular, levels of prox-1, the ortholog of Drosophila transcription factor prospero, and cofilin-1, a regulator of the actin cytoskeleton, were reduced. During normal metanephrogenesis, prox-1 protein was first detected in mesenchymal cells around the ureteric tree and thereafter in nascent nephron epithelia, whereas cofilin-1 immunolocalized to bud derivatives and condensing mesenchyme. Previously, we reported that low-protein diets increased mesenchymal apoptosis cells when metanephrogenesis began and thereafter reduced numbers of precursor cells. Collectively, these studies prove that the maternal diet programs the embryonic kidney, altering cell turnover and gene expression at a time when nephrons and glomeruli have yet to form. The human implication is that the maternal diet ingested between conception and 5- 6-wk gestation contributes to the variation in glomerular numbers that are known to occur between healthy and hypertensive populations.  (+info)

Micropuncture gene delivery and intravital two-photon visualization of protein expression in rat kidney. (5/145)

Understanding molecular mechanisms of pathophysiology and disease processes requires the development of new methods for studying proteins in animal tissues and organs. Here, we describe a method for adenoviral-mediated gene transfer into tubule or endothelial cells of the rat kidney. The left kidney of an anesthetized rat was exposed and the lumens of superficial proximal tubules or vascular welling points were microinfused, usually for 20 min. The microinfusion solution contained adenovirus with a cDNA construct of either 1) Xenopus laevis actin depolymerizing factor/cofilin [XAC; wt-green fluorescent protein (GFP)], 2) actin-GFP, or 3) GFP. Sudan black-stained castor oil, injected into nearby tubules, allowed us to localize the microinfused structures for subsequent visualization. Two days later, the rat was anesthetized and the kidneys were fixed for tissue imaging or the left kidney was observed in vivo using two-photon microscopy. Expression of GFP and GFP-chimeric proteins was clearly seen in epithelial cells of the injected proximal tubules and the expressed proteins were localized similarly to their endogenously expressed counterparts. Only a minority of the cells in the virally exposed regions, however, expressed these proteins. Endothelial cells also expressed XAC-GFP after injection of the virus cDNA construct into vascular welling points. An advantage of the proximal tubule and vascular micropuncture approaches is that only minute amounts of virus are required to achieve protein expression in vivo. This micropuncture approach to gene transfer of the virus cDNA construct and intravital two-photon microscopy should be applicable to study of the behavior of any fluorescently tagged protein in the kidney and shows promise in studying renal physiology and pathophysiology.  (+info)

Ligation of cell surface-associated glucose-regulated protein 78 by receptor-recognized forms of alpha 2-macroglobulin: activation of p21-activated protein kinase-2-dependent signaling in murine peritoneal macrophages. (6/145)

Previous studies of the plasma proteinase inhibitor alpha2-macroglobulin (alpha2M) demonstrated that alpha2M-proteinase complexes (alpha2M*) modulate immune responses and promotes macrophage locomotion and chemotaxis. Alpha2M* binds to cell surface-associated glucose-regulated protein 78 (GRP78), which activates downstream signaling events. The role of p21-activated protein kinase-1 and -2 (PAK-1 and -2) in promoting cellular motility is well documented. In the current study, we examined the ability of alpha2M* to activate PAK-1 and PAK-2. Upon macrophage stimulation with alpha2M*, PAK-2 is autophosphorylated, resulting in increased kinase activity; however, PAK-1 is negligibly affected. Alpha2M*-stimulated macrophages showed a marked elevation in the levels of Rac x GTP. Receptor tyrosine phosphorylation upon binding of alpha2M* to GRP78, recruits PAK-2 to the plasma membrane via the adaptor protein NCK. Consistent with this hypothesis, silencing of GRP78 gene expression greatly attenuated the levels of membrane-associated PAK-2 and NCK. PAK-2 activity was markedly decreased by inhibition of tyrosine kinases and PI3K before alpha2M* stimulation. We further demonstrate that phosphorylation of Lin-11, Isl-1, Mec-3 (LIM) kinase and cofilin is promoted by treating macrophages with alpha2M*. Thus, alpha2M* regulates activation of the PAK-2-dependent motility mechanism in these cells.  (+info)

Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C. (7/145)

BACKGROUND: Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS: A method for DNA splicing and mutagenesis without restriction enzymes is described. The method is based on mild template-dependent polymerization arrest with two molecules of cytosine arabinose (Ara-C) incorporated into PCR primers. Two rounds of PCR are employed: the first PCR produces 5' overhangs that are utilized for DNA splicing. The second PCR is based on polymerization running through the Ara-C molecules to produce the desired final product. To illustrate application of the run through stop mutagenesis and DNA splicing technique, we have carried out splicing of two segments of the human cofilin 1 gene and introduced a mutational deletion into the product. CONCLUSION: We have demonstrated the utility of a new PCR-based method for carrying out DNA splicing and mutagenesis by incorporating Ara-C into the PCR primers.  (+info)

Stimulation of actin polymerization by filament severing. (8/145)

The extent and dynamics of actin polymerization in solution are calculated as functions of the filament severing rate, using a simple model of in vitro polymerization. The model is solved by both analytic theory and stochastic-growth simulation. The results show that severing essentially always enhances actin polymerization by freeing up barbed ends, if barbed-end cappers are present. Severing has much weaker effects if only pointed-end cappers are present. In the early stages of polymerization, the polymerized-actin concentration grows exponentially as a function of time. The exponential growth rate is given in terms of the severing rate, and the latter is given in terms of the maximum slope in a polymerization time course. Severing and branching are found to act synergistically.  (+info)

DNA constructs, reagents, and antibodies. Full-length human SSH1L cDNA was amplified by PCR using the RZPD clone IRAUp969F0865D6 as a template and cloned into pCR3.V62-Met-Flag and pEGFP-N3 vectors. The SSH1L point mutant was generated by site-directed PCR mutagenesis. Integrity of all constructs was verified by sequencing. Antibodies used were as follows: anti-PKD2 rabbit polyclonal antibody (Calbiochem), anti-PKD C-20 (Santa Cruz Biotechnology), anti-phospho-cofilin (Ser3) and anti-cofilin rabbit polyclonal antibodies (Cell Signaling), anti-Flag M2 mouse monoclonal antibody (Sigma-Aldrich, Germany), anti-tubulin mouse monoclonal antibody (Neomarkers), anti-SSH1L rabbit polyconal antibody (Abcam), and anti-glutathione S-transferase (GST) goat polyclonal antibody (GE Healthcare). Secondary antibodies used were horseradish peroxidase-coupled donkey anti-goat, goat anti-mouse, and anti-rabbit IgGs (Dianova). Alexa546- and Alexa633-coupled phalloidin were from Invitrogen.. Cell culture. Cells were ...
During early metastatic events in cancer, reorganization of the actin cytoskeleton must occur to allow for cell invasion and migration. A key enzyme initiating actin remodeling at the leading edge is Cofilin, a small actin binding protein of the ADF/cofilin family with actin severing activities. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation events that are mediated by LIM Kinase (LIMK) and the phosphatase Slingshot (SSH), respectively. Protein kinase D (PKD) is a serine/threonine kinase that has previously been shown to regulate actin driven directed cell migration through phosphorylation and thus inactivation of Slingshot. Here we show that PKD can also regulate cofilin activity via activation of LIMK. We further show that PKD-induced regulation of LIMK and the resulting diminished cofilin activity translates to altered cell motility. Our data suggest that PKD inactivates Cofilin through modulation of both of its regulatory pathways. Further elucidation of the ...
Cofilin 1 (non-muscle; n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. One group reports that reelin signaling leads to serine3-phosphorylation of cofilin-1, and this interaction may play a role in the reelin-related regulation of neuronal migration. Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000056201 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: CFL1 cofilin 1 (non-muscle). Chai X, Förster E, Zhao S, Bock HH, Frotscher M (January 2009). Reelin stabilizes the actin cytoskeleton of neuronal processes by inducing n-cofilin ...
Endothelin-1 (ET-1) is a neuroactive peptide produced by neurons, reactive astrocytes, and endothelial cells in the brain. Elevated levels of ET-1 have been detected in the post-mortem brains of individuals with Alzheimers disease (AD). We have previously demonstrated that overexpression of astrocytic ET-1 exacerbates memory deficits in aged mice or in APPK670/M671 mutant mice. However, the effects of ET-1 on neuronal dysfunction remain elusive. ET-1 has been reported to mediate superoxide formation in the vascular system via NADPH oxidase (NOX) and to regulate the actin cytoskeleton of cancer cell lines via the cofilin pathway. Interestingly, oxidative stress and cofilin activation were both reported to mediate one of the AD histopathologies, cofilin rod formation in neurons. This raises the possibility that ET-1 mediates neurodegeneration via oxidative stress- or cofilin activation-driven cofilin rod formation. Here, we demonstrate that exposure to 100 nM ET-1 or to a selective ET type B ...
The actin depolymerizing factor (ADF)/cofilin protein family is essential for actin dynamics, cell division, chemotaxis and tumor metastasis. Cofilin-1 (CFL-1) is a primary non-muscle isoform of the ADF/cofilin protein family accelerating the actin filamental turnover in vitro and in vivo. In response to environmental stimulation, CFL-1 enters the nucleus to regulate the actin dynamics. Although the purpose of this cytoplasm-nucleus transition remains unclear, it is speculated that the interaction between CFL-1 and DNA may influence various biological responses, including DNA damage repair. In this review, we will discuss the possible involvement of CFL-1 in DNA damage responses (DDR) induced by ionizing radiation (IR), and the implications for cancer radiotherapy.
Supplementary Materialsoncotarget-06-1834-s001. cofilin (Ser 3) and Drp1 (Ser 637) are translocated to the mitochondria. Cofilin S3E and Drp1 S637D mutants, which mimick the phosphorylated forms, suppressed mitochondrial translocation, fission, and apoptosis. Moreover, both dephosphorylation and mitochondrial translocation of cofilin and Drp1 are dependent on ROCK1 activation. findings confirmed that erucin-mediated inhibition of tumor growth in a breast malignancy cell xenograft mouse model is usually associated with the mitochondrial translocation of cofilin and Drp1, fission and apoptosis. Our study reveals a novel role of cofilin in regulation of mitochondrial fission and suggests erucin as a potential drug for treatment of breast malignancy. [21, 22] and in tumor xenograft models [23]. The results of recent studies suggest that a mitochondrion-dependent pathway may play an important part in erucin-mediated apoptosis [24]. However, the molecular mechanisms by which erucin regulates the ...
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A) PirB interacts with cofilin in vivo in PirB+/− Tg mice (P30, forebrain), assessed by immunoprecipitation for PirB. Other known PirB-proximal signaling and interactions such as tyrosine phosphorylation of PirB and SHP-2 recruitment to PirB are not altered in PirB+/− relative to PirB+/− Tg mice. Representative data are shown (n , 2 ...
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Cofilin 2 antibody (cofilin 2 (muscle)) for WB. Anti-Cofilin 2 pAb (GTX14133) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
Mouse Monoclonal Anti-Cofilin Antibody (1A1) [DyLight 755]. Validated: WB, ELISA, ICC/IF, IHC, IHC-P. Tested Reactivity: Human, Mouse. 100% Guaranteed.
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
MAPK continues to be proven to limit activation of MAPK 経路 LIMK, subsequent phosphorylation of cofilin, and migration of major human T cells in the three dimen
Having an existing gmfgen file and a generated diagram plug-in, but trying to generate the same diagram plug-in results in different java classes structure ...
Having an existing gmfgen file and a generated diagram plug-in, but trying to generate the same diagram plug-in results in different java classes structure ...
The IS is a polarized cell structure similar to the leading edge of a migrating cell or the yeast bud site. Establishing functional patterns of membrane protein organization and vesicular traffic at these sites requires coordinated reorganization of the actin and microtubule cytoskeletons. Rap1 is an evolutionarily conserved regulator of cell polarity that drives actin reorganization at the B cell IS (Lin et al., 2008). We have now shown that Rap1, and its downstream target, the actin-severing protein cofilin, are essential for BCR-induced MTOC polarization towards the IS. Whether Rap1 controls MTOC reorientation in other immune cells is not known. The BCR and TCR both activate Rap1 via phospholipase Cγ-dependent production of diacylglycerol (Katagiri et al., 2004; McLeod et al., 1998), which recruits the Rap1 exchange factor RasGRP2 (also known as CalDAG-GEFI) (Kawasaki et al., 1998) to the plasma membrane. The scaffolding protein ADAP (also known as FYB), which promotes the accumulation or ...
To make Angry Birds a real sport, Mbed made a USB slingshot to play Angry Birds. The slingshot emulates a USB mouse, so it really is a plug n play. It translates the physical use of the slingshot in to appropriate mouse controls. The way the controller works is reasonably simple: a stretch sensor translates the drawing of the slingshot to a mouse movement and an accelerometer determines the angle. Check out the video of it in action. ...
SLINGSHOT™: Athletic Performance and Endurance Maximizer SLINGSHOT™ YOUR PERFORMANCE TO THE NEXT LEVEL! SLINGSHOT™ athletic
Alzheimers disease (AD) is a neurodegenerative disorder characterized by two major pathological hallmarks, amyloid plaques and neurofibrillary tangles. The accumulation of amyloid-β protein (Aβ) is an early event associated with synaptic and mitochondrial damage in AD. Therefore, molecular pathways underlying the neurotoxicity and generation of Aβ represent promising therapeutic targets for AD. Recent studies have shown that actin severing protein, Cofilin plays an important role in synaptic remodeling, mitochondrial dysfunction, and AD pathogenesis. However, whether Cofilin is an essential component of AD pathogenesis and how Aβ induced neurotoxicity impinges its signals to Cofilin are unclear. In my dissertation studies, we found Aβ oligomers bind with intermediate activation conformers of β1-integrin to induce the loss of surface β1-integrin and activation of Cofilin via Slingshot homology-1 (SSH1) activation. Specifically, conditional loss of β1-integrin prevented Aβ induced Cofilin
Cofilin belongs to the actin-binding ADF protein family. It contains one ADF-H domain. Cofilin controls reversible actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin at a 1:1 ratio. It is the major component of both intranuclear and cytop...
Real‐time imaging. Cells were seeded in a Lab‐Tek Chambered #1.0 Borosilicate Coverglass System (Nalgene Nunc International, Rochester, NY, USA) and were mounted on a Zeiss Axiovert 200M microscope for live‐cell imaging (5% CO2; 37°C) for 2-14 h. Phase‐contrast images were captured every 2 min using a Photometics Coolsnap CCD camera (Scientific, Tuscon, AZ, USA). Images were processed using the Metamorph software (Universal imaging, Downington, PA, USA). Several protrusions was scored by off‐line analysis of the generated videos and plotted as means±s.e.m. For real‐time fluorescence imaging, cells were placed on a Leica SP5 inverted microscope equipped with a × 63 1.3NA glycerol objective. Cells were maintained in Leibovitz‐15‐buffered medium (Invitrogen, Breda, The Netherlands) at 37°C in a climate chamber.. Invasion assay. For in vitro invasion assays, 24‐well BioCoat matrigel invasion chambers (#354480, BD Biosciences, Alphen aan den Rijn, The Netherlands) were used ...
PAK4_HUMAN] Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of ...
If the day ever comes when you need to find your own food, like really find your own food, a slingshot might come in handy. Think about it: If things are that bad that you need to hunt your own game, theres also a pretty good chance you wont have unlimited rifle ammo. This Survival Slingshot is configured for archery, with 25 pounds of pull sending arrows hurtling through the air at your meal-to-be. And with a mounted tactical light, even owls better watch their backs and fronts.. ...
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Actin-severing proteins ADF/cofilin are required for the sorting of secretory cargo at the trans-Golgi network (TGN) in mammalian cells. How do these cytoplasmic proteins interact with the cargoes in the lumen of the TGN ...
Reporting on the project, Dr Goldsbury wrote, In this project supported by the SZCUF, we have demonstrated that reduced energy metabolism by inhibition of mitochondrial function in cultured neurons gives rise to the redistribution of tau and cofilin into rod-like structures that appear to mimic those in the AD brain. Further, we demonstrated that the density of cofilin rods in human AD brains is significantly higher than in normal aged human brain. However, we also showed that some aged normal brains contained low levels of cofilin rods, although these brains were devoid of abnormal tau. This suggests that cofilin rod generation may be an early event in the neurodegenerative cascade representing a possible early-stage mechanism in the progression to AD dementia and therefore an ideal future target for therapeutic intervention that might be useful in treatment of neurological diseases. Unexpectedly, however, despite an extensive study, we were not able to show any substantial microscopic overlap ...
One candidate early on was a protein called slingshot, which seemed to have the correct phosphatase activity. However, slingshot turned out not to be found in all organisms, nor ubiquitously distributed throughout the body, a requirement for the mystery phosphatase, as it would be needed wherever actin dynamics is taking place. Additionally, slingshot did not appear to be capable of regulating all cofilin-dependent cell processes, so clearly a third man still existed.. Now in an article recently published in Nature Cell Biology, Bokoch and his colleagues Antje Gohla and Jörg Birkenfeld argue that the third man phosphatase is actually chronophin. Taking the increasingly overlooked approach of function-based biochemical isolation, which required Gohla to spend significant time in the cold room, the scientists purified a phosphatase activity which dephosphorylated cofilin. This activity was then identified by mass spectrometry as chronophin, a member of a unique superfamily of proteins called ...
Beautiful and functional, the DDMWorks aluminum shift knob offers extremely smooth and clean styling when installed. The knob is designed with a 2 round top that feels natural in your hand. The knob is CNC machined from a solid piece of billet aluminum. This single piece design is specifically made for the Polaris Slingshot and installs in just minutes. ...
Houston Kiteboarding says:Love this board! Not only the lightest board slingshot has ever made but the lightest board Ive ever been on. Feels great on the water and very similar stiffness and pop to the older versions of the carbon Crazyfly Raptor...which is a good thing. Loved every minute of it. The flex Id sa
These two friends thought taking a ride on the Slingshot at the 2020 Houston Rodeo would be fun, too bad they probably dont remember much of it as it puts them both to sleep.
Best day pack ever. Buy it today. I bought my TNF Slingshot in 2007 and it is still in near perfect condition. I drive a Jeep Wrangler with no top, doors or windows so a bag is necessary to carry everything I have at any given...
The SR-7 Wrist-Rocket® is a durable entry level slingshot. Molded handle Self centering pouch Tubular bands with protective prong caps to extend band life Made …
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We were very curious about what this dealer project would cost to the driver, as they estimated the modifications on the car would add up to around $30,000 (well let that sink it for a moment). This would put the Slingshot Trans Am at $65,000 brand new. Someone thought it was well worth it; this Slingshot Trans Am has been collector owned since it was new, and only has 1,544 miles on it - its still in showroom condition.. The original owner had chosen to never really open this car up, but hey, its their car to do as they like. Its never actually seen a track, its spent most of its time in a climate-controlled collectors garage for the past 14-years. Some might consider it a waste since its likely one of the best-optioned 4th-gens to leave a dealership, but collectors are all about preservation and keeping the vehicles safe from the dangers of the roads and tracks.. We do, however, suspect that this car may has since been sold to a second owner, and possible subsequent owners. Weve seen ...
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Three dimensional protein models of ADF/CFL proteins highlighting sites with accelerated rates of evolution (ω3 rate category) in pink.
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This Forum contains Member Profiles. These members have achieved Hall of Fan Status (5000 posts) and as such have demonstrated their commitment to the CFL and 13thman.com. Hall of Fan Members will of course receive respect and admiration from your peers and have the opportunity to place your profile here ...
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I would have gone, but I couldnt find a slingshot big enough. Oh, and I didnt realize there was a Cow Con III. But if it wasnt for those two things, I might have gone ...
The GAP first draft also has added the elements of NCDs Global Monitoring Framework (including twenty-five indicators) and Voluntary Global Targets (nine targets) finalised in the WHO meeting of member states late last year (5-7 November 2012). However the stakeholders want the 25 indicators of Global Monitoring Framework (GMF) and 9 voluntary global targets should be integrated in GAP alongwith evidence based interventions. Let us examine the GMF and GAP in some detail: ...
Also in the mix is a DC-DC converter to drop the 60 volts down to 12 volts for the handful of accessories that are in the plans. Plug-In Electric Car …
Ive updated this post from 2010 to include the correct link and new information! Also, please do not leave your address or account number in the comments below!
For the SSH-1 protocol, see Secure Shell#Version 1.x Protein phosphatase Slingshot homolog 1 is an enzyme that in humans is encoded by the SSH1 gene. The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002).[supplied by OMIM] GRCh38: Ensembl release 89: ENSG00000084112 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000042121 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O (Apr 2000). Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7 (1): 65-73. doi:10.1093/dnares/7.1.65. PMID 10718198. Niwa ...
Cofilin phospho-regulation is important for actin filament turnover and is implicated in cancer. Phosphorylation of cofilin is mediated by LIM kinases (LIMKs) and dephosphorylation by Slingshot phosphatases (SSH). LIMKs and SSH promote cancer cell invasion and metastasis and represent novel anti-cancer targets. However, little is known regarding LIMK/cofilin and SSH in human colorectal cancer (CRC). In this study, we aimed to address their expression and significance in human CRC. We evaluated expression of non-phosphorylated (active) and phosphorylated cofilin, LIMK1, LIMK2, and SSH1 by immunohistochemistry in 143 human CRC samples in relation to clinicopathologic parameters, response of metastatic disease to chemotherapy, and epithelial-mesenchymal transition (EMT) markers β-catenin, E-cadherin, and ZEB ...
A model from Chile, Maura Bellagamba, released a video on Instagram of herself being released in a giant slingshot, which she says caused her neck injuries while filming a Nissan car commercial.. Bellagamba wrote on Instagram that she was fired out of the slingshot without her permission, causing her whiplash ...
Remodeling of the actin cytoskeleton is involved in all motile and membrane trafficking processes, including cell migration, phagocytosis, macropinocytosis, endocytosis, exocytosis, and last, not least autophagy. This explains why actin and actin polymerization regulators constitute prime targets of bacterial pathogens, which have evolved to manipulate signaling pathways to actin cytoskeleton remodeling at various key nodes, foremost upstream Rho GTPase signaling, but also downstream actin polymerization and depolymerization factors. In my talk, I will address the molecular mechanisms of host cell responses to well-characterized Salmonella enterica virulence factors, including members of the Sop (Salmonella outer protein) family of proteins translocated into the host, and from there move on to less well understood mediators of bacterial pathogenesis and effects. ...
Gentaurs CFL1 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CFL1. Standards or samples are added to the micro ELISA plate wells and combined with ...
To confirm the morphological effects of 5-HT6Rs transfected in neuronal cells, we finally examined whether the activation of neuronal 5-HT6Rs produces effects on RhoA activities. To perform RhoA activity assay as shown in Fig. 2, we carried immunoblot analysis using cultured cortical neurons for sufficient amount of sample proteins. Here, we showed that 5-HT6R activation by ST1936 significantly increased RhoA-GTP level, which was significantly blocked by the pretreatment with SB258585 in cultured neurons (Figs. 4A and 4B). As downstream targets of Rho proteins, RhoA proteins can also affect actin polymerization by regulating cofilin via Rho-ROCK-LIMK-cofilin pathway (Lin et al., 2003). Therefore, we further executed whether the activation of 5-HT6Rs modulates cofilin activities as a downstream target of RhoA. As shown in Figs. 4C and 4D, we observed that treatment with ST1936 significantly increased phosphorylation of cofilin, which was also significantly blocked by SB258585 in cultured neurons. ...
To confirm the morphological effects of 5-HT6Rs transfected in neuronal cells, we finally examined whether the activation of neuronal 5-HT6Rs produces effects on RhoA activities. To perform RhoA activity assay as shown in Fig. 2, we carried immunoblot analysis using cultured cortical neurons for sufficient amount of sample proteins. Here, we showed that 5-HT6R activation by ST1936 significantly increased RhoA-GTP level, which was significantly blocked by the pretreatment with SB258585 in cultured neurons (Figs. 4A and 4B). As downstream targets of Rho proteins, RhoA proteins can also affect actin polymerization by regulating cofilin via Rho-ROCK-LIMK-cofilin pathway (Lin et al., 2003). Therefore, we further executed whether the activation of 5-HT6Rs modulates cofilin activities as a downstream target of RhoA. As shown in Figs. 4C and 4D, we observed that treatment with ST1936 significantly increased phosphorylation of cofilin, which was also significantly blocked by SB258585 in cultured neurons. ...
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For 2020, we stripped our existing Dually platform down to the bones and rebuilt it from the toes up. The result is a reimagined and reengineered binding that provides better grip, leverage and board control, more reactive handling and happier feet. Now offered in two sizes for a better fit across more feet sizes and s
You will never see with a slingshot shoot as accurately as this one! A mobile sight design that can quickly lock the target. The spring pressure design makes it very convenient and quick to replace the rubber band. The curved handle and man-made mechanical design make it very comfortable to hold in the hand. The slings
Barbe Rousse, the artist, has a lot of interesting and unusual artworks connected with anatomy. The Bone Slingshot Tattoo is one of them, and it looks awesome.
EVO is designed and built to make your production life easier, which means you get purpose built features like Slingshot and ShareBrowser. With Slingshot, an Automations GUI and API, you can create and run automated file workflows that span EVO, other storage systems, and Amazon S3/Glacier cloud services. Slingshot helps boost productivity by enabling you to automate repetitive file management tasks, leaving you and your team with more valuable time in post. ShareBrowser is an Easy-to-Use, unified file/project/asset management interface for macOS and Windows. It is similar to Finder/Explorer, but designed by SNS specifically to help media teams work and collaborate more efficiently. The ShareBrowser Desktop Client not only improves visibility and access to your teams storage systems, it conveniently enables automatic project locking for FCP, Premiere Pro, and Pro Tools - as well as Avid project and bin sharing. enables your team to search across all EVO storage as well as local, offline, and ...
Abstract: A key aspect of cell and tissue morphogenesis is regulation of the actin cytoskeleton. The actin cytoskeleton is a fundamental cellular scaffold which...
Fads remind me of an iPad game called Slingshot Racing. What you do is, there are these tiny cars that run around a tiny oval racetrack, and you have to click on the screen (tech faux pas! tap on the screen) to tether the cars to tiny towers that slingshot them around the corners (ends? short sides?) of the oval. It sounds easy but in fact its devastatingly hard to tap and release at exactly the right times to start the tether and stop it without smashing your tiny car into a tiny wall. Thats kind of like catching and riding a fad. You have to discover that the potential for a fad exists, and not just any fad; one you can make money on, say by selling rocks or playing the saxophone in a particular way. You have to tether the fad to you at just the right moment, then hold onto it just up until the moment when its about to fade, at which point you release it before you start looking stupid. Few succeed at this game. Some tap too early and suffer ridicule, that is, until after theyre dead and ...
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Ladies, and gentleman, I would like to present the winner of the Grey Cup, this year... Buffalo! Although the idea of a CFL commissioner making such a statement in the future seems far fetched, ...
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J:194042 Mahaffey JP, Grego-Bessa J, Liem KF Jr, Anderson KV, Cofilin and Vangl2 cooperate in the initiation of planar cell polarity in the mouse embryo. Development. 2013 Mar;140(6):1262-71 ...
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2019- 2020學年(上學期)????? (2019.10.14-2019.10.18)課題組名稱(聯系人)時? 間地? 點主講人題?? 目理論物理研究所 (李世淵)星期三15:30知新樓C913??交叉科學研究所 (劉向東)星期二下午離子束報告廳????????原子分子物理 (許國富)星期三下午知新樓C1011??????????凝聚態理論研究室 (崔彬)星期四14:30知新樓C1113季藝聞基于SSH模型對A-D-A類分子建模????電介質物理研究室 (趙明磊)星期五14:30知新樓C1113??...
... has been shown to interact with Cofilin 1. GRCh38: Ensembl release 89: ENSG00000120694 - Ensembl, May 2017 GRCm38: ... 869 (1-2): 49-55. doi:10.1016/S0006-8993(00)02346-5. PMID 10865058. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ... 1444 (1): 138-42. doi:10.1016/s0167-4781(98)00254-1. PMID 9931472. "Entrez Gene: HSPH1 heat shock 105kDa/110kDa protein 1". ... The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA ...
... signaling leads to phosphorylation of actin-interacting protein cofilin 1 at ser3; this may stabilize the actin ... "Reelin stabilizes the actin cytoskeleton of neuronal processes by inducing n-cofilin phosphorylation at serine3". The Journal ... 112 (1-2): 33-45. doi:10.1016/S0169-328X(03)00032-9. PMID 12670700. Hack I, Hellwig S, Junghans D, Brunne B, Bock HH, Zhao S, ... 1 (2): 72-73. doi:10.1046/j.1535-7597.2001.00017.x. PMC 320825. PMID 15309195. Zaki M, Shehab M, El-Aleem AA, Abdel-Salam G, ...
In both yeast and mammals, CAPs appear to be involved in recycling G-actin monomers from ADF/cofilins for subsequent rounds of ... Moriyama K, Yahara I (April 2002). "Human CAP1 is a key factor in the recycling of cofilin and actin for rapid actin turnover ... A second unique function of CAP in regulating actin dynamics is its ability to specifically bind to pointed ends of cofilin- ... Shekhar S, Chung J, Kondev J, Gelles J, Goode BL (22 November 2019). "Synergy between Cyclase-associated protein and Cofilin ...
ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... 2005). "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin". EMBO J. 24 (3): ... 2007). "The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and ...
Vallenius, T; Luukko K; Mäkelä T P (Apr 2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha- ... Otey CA, Pavalko FM, Burridge K (1990). "An interaction between alpha-actinin and the beta 1 integrin subunit in vitro". J. ... Vallenius T, Luukko K, Mäkelä TP (2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 ... An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin". Eur. J. Biochem. 268 (23): 6165-75. doi: ...
Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (Jun 1998). "Cofilin phosphorylation by LIM- ... Sumi T, Matsumoto K, Takai Y, Nakamura T (27 Dec 1999). "Cofilin phosphorylation and actin cytoskeletal dynamics regulated by ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (Aug 2001). "Cofilin phosphorylation and actin reorganization activities of ... Vardouli L, Moustakas A, Stournaras C (Mar 2005). "LIM-kinase 2 and cofilin phosphorylation mediate actin cytoskeleton ...
ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... a family of phosphatases that dephosphorylate ADF/cofilin". Cell. 108 (2): 233-46. doi:10.1016/S0092-8674(01)00638-9. PMID ... "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin". The EMBO Journal. 24 (3): ...
2004). "A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia". J. Cell Biol ... ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... 2007). "Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge". Cell. 128 (5): 915-29. doi:10.1016/j ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ...
Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (1998). "Cofilin phosphorylation by LIM-kinase 1 ... Birkenfeld J, Betz H, Roth D (January 2003). "Identification of cofilin and LIM-domain-containing protein kinase 1 as novel ... Nebl G, Meuer SC, Samstag Y (1996). "Dephosphorylation of serine 3 regulates nuclear translocation of cofilin". J. Biol. Chem. ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of ...
"Prion-like protein aggregates exploit the RHO GTPase to cofilin-1 signaling pathway to enter cells". The EMBO Journal. 37 (6). ... One of the inhibitors discovered in Bertolotti's lab, Sephin1, has passed through favourable Phase 1 clinical trials in 2019 ... Münch, Christian; O'Brien, John; Bertolotti, Anne (2011-03-01). "Prion-like propagation of mutant superoxide dismutase-1 ... "Exposure of hydrophobic surfaces initiates aggregation of diverse ALS-causing superoxide dismutase-1 mutants". Journal of ...
Birkenfeld J, Betz H, Roth D (January 2003). "Identification of cofilin and LIM-domain-containing protein kinase 1 as novel ... 9 (1): 143-54. doi:10.1089/jam.1996.9.143. PMID 10160204. Filoche M, Tai CF, Grotberg JB (July 2015). "Three-dimensional model ... 369 (Pt 1): 45-54. doi:10.1042/BJ20021152. PMC 1223062. PMID 12323073. Waterman MJ, Stavridi ES, Waterman JL, Halazonetis TD ( ... Koyama S, Williams LT, Kikuchi A (July 1995). "Characterization of the interaction of Raf-1 with ras p21 or 14-3-3 protein in ...
Enhanced docetaxel-mediated cytotoxicity in human prostate cancer cells through knockdown of cofilin-1 by carbon nanohorn ... 1 July 2016). "Design of Assembled Systems Based on Conjugated Polyphenylene Derivatives and Carbon Nanohorns". Chemistry - A ...
"Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization". Nature Cell Biology. 9 (3): 276 ... Production of IL-1β downstream of caspase-11 requires another canonical inflammasome, called the NLRP3 inflammasome, that ... The CARD domain of caspase-11 has been shown to associate with AIP-1 and cofilin to facilitate actin depolymerization. In ... This mechanism contrasts to that of the canonical inflammasome, in which a bacterial ligand activates caspase-1 through an ...
Toshima J, Toshima JY, Amano T, Yang N, Narumiya S, Mizuno K (2001). "Cofilin phosphorylation by protein kinase testicular ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of ... Dual specificity testis-specific protein kinase 1 is an enzyme that in humans is encoded by the TESK1 gene. This gene product ... "Entrez Gene: TESK1 testis-specific kinase 1". Toshima JY, Toshima J, Watanabe T, Mizuno K (November 2001). "Binding of 14-3- ...
... cofilin 1 MeSH D12.776.220.525.212.750 - cofilin 2 MeSH D12.776.220.525.212.875 - destrin MeSH D12.776.220.525.246.500 - actin- ... neuregulin-1 See List of MeSH codes (D12.776.476). MeSH D12.776.503.280.249.500 - mannose-binding lectin MeSH D12.776.503.280. ... hiv-1 reverse transcriptase MeSH D12.776.964.970.880.325 - gene products, env MeSH D12.776.964.970.880.325.330 - hiv envelope ... neurofibromin 1 MeSH D12.776.402.150.500.500 - p120 gtpase activating protein MeSH D12.776.402.300.700.500 - ras-GRF1 MeSH ...
... cofilin 2 MeSH D05.750.078.730.212.875 - destrin MeSH D05.750.078.730.246 - actin-related protein 2-3 complex MeSH D05.750. ...
The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of ... Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever ... Destrin binds actin and is thought to connect it as gelsolin segment-1 does. ...
... like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans". Biomolecular NMR Assignments. 9 (2): 261-265. doi: ... Shukla, Vaibhav Kumar; Kabra, Ashish; Yadav, Rahul; Ono, Shoichiro; Kumar, Dinesh; Arora, Ashish (1 October 2015). "NMR ... 1 February 2017). "Unraveling the stereochemical and dynamic aspects of the catalytic site of bacterial peptidyl-tRNA hydrolase ...
Cofilin 2 (muscle) also known as CFL2 is a protein which in humans is encoded by the CFL2 gene. Cofilin is a widely distributed ... Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN), all of which regulate ... "Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14". Ann. Hum ... 2006). "Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface". J. Mol. Biol. ...
... n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular ... "Entrez Gene: CFL1 cofilin 1 (non-muscle)". Chai X, Förster E, Zhao S, Bock HH, Frotscher M (January 2009). "Reelin stabilizes ... Lee K, Jung J, Kim M, Guidotti G (2001). "Interaction of the alpha subunit of Na,K-ATPase with cofilin". Biochem. J. 353 (Pt 2 ... Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 " ...
In accordance with their proposed rule, proteins like ADF, cofilin and LIMK1 are slingshot substrates. Phosphatases of ... Regenerating Liver (PRLs): Three PRL genes were described in mammals (PRL-1, PRL-2 and PRL-3). They share a high sequence ...
The latter is the enzyme that uses ATP to phosphorylate and inactivate the actin-depolymerizing factor cofilin. When cofilin is ... In the presence of Pyr1, LIMK1 is inhibited, which means that the phosphorylation of cofilin decreases, which results in the ... Pyr 1 is a cell permeable competitive inhibitor of Lim Kinase (especially LIMK1). ...
ADF/cofilin are the only substrates yet identified for the LIM kinases. LIM kinases directly phosphorylate and inactivate ... are actin-binding kinases that phosphorylate members of the ADF/cofilin family of actin binding and filament severing proteins ... LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal ... members of the cofilin family, resulting in stabilization of filamentous (F)-actin. Lim kinases are activated by signaling ...
Meanwhile, phosphorylation of PI3K by SRC activates RhoA which leads to activation of cofilin-P and disruption of filamentous ... When the WAVE-1 gene was disrupted in mice, it resulted in cognitive defects such as losses in learning and memory implicating ... 28 (1): 245-259. doi:10.1016/S0896-6273(00)00100-8. PMID 11086998. Tanaka, H; Shan, W; Phillips, GR; Arndt, K; Bozdagi, O; ... 51 (1): 43-56. doi:10.1016/j.neuron.2006.05.018. PMC 2587166. PMID 16815331. Ohkubo, T; Ozawa, M. (1999). "p120ctn Binds to the ...
... which then stimulates cofilin, which effectively reorganizes the actin cytoskeleton of the cell. In the case of neurons, ... The LINGO-1 structure has been shown to be highly stable both in its crystal form and in solution, thanks to its leucine-rich ... LINGO-1 is coded by the LINGO-1 gene, which is located on the human chromosome 15, more precisely on the locus 15q24-26, which ... LINGO-1 is expressed almost exclusively in the central nervous system (CNS). It can be found in the brain and in neurons and ...
2005). "Ribosomal protein S18 identified as a cofilin-binding protein by using phage display library". Mol. Cell. Biochem. 262 ... 239 (1): 144-9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID 8706699. Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed ... 12 (1): 1-11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033. Mishra-Gorur K, Singer HA, Castellot JJ (2002 ... 1-2): 187-93. doi:10.1023/B:MCBI.0000038234.35936.1c. PMID 15532723. S2CID 30534195. Andersen JS, Lam YW, Leung AK, et al. ( ...
1999). "Aip1p interacts with cofilin to disassemble actin filaments". J. Cell Biol. 145 (6): 1251-64. doi:10.1083/jcb.145.6. ... 327 (1): 268-75. doi:10.1016/j.bbrc.2004.11.156. PMID 15629458. Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a ... 56 (1): 59-69. doi:10.1006/geno.1998.5672. PMID 10036186. "Entrez Gene: WDR1 WD repeat domain 1". Dawson SJ, White LA (1992). " ... 23 (1): 94-101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157. Fujibuchi T, Abe Y, Takeuchi T, et al. (2005). "AIP1/WDR1 ...
"Memo is a cofilin-interacting protein that influences PLCgamma1 and cofilin activities, and is essential for maintaining ... 78 (1-2): 46-54. doi:10.1006/geno.2001.6640. PMID 11707072. Qiu C, Lienhard S, Hynes NE, Badache A, Leahy DJ (February 2008). " ... "Entrez Gene: Mediator of cell motility 1". Meira M, Masson R, Stagljar I, Lienhard S, Maurer F, Boulay A, Hynes NE (March 2009 ... Mediator of cell motility 1 is a protein that in humans is encoded by the MEMO1 gene. GRCh38: Ensembl release 89: ...
It causes a reduction of cell multiplication.[citation needed] This triterpene can inhibit the phosphorylation of the cofilin ... Drubin, D. G.; Lappalainen, P. (1997). "Cofilin promotes rapid actin filament turnover in vivo". Nature. 388 (6637): 78-82. ... "Cucurbitacin E as a new inhibitor of cofilin phosphorylation in human leukemia U937 cells". Bioorganic & Medicinal Chemistry ... 364 (1): 181-186. doi:10.1016/j.bbrc.2007.09.075. PMID 17942079. Clancy, D.; Birdsall, J. (2012). "Flies, worms and the Free ...
核纤层蛋白 A/C(英语:Lamin A/C)是由人类基因LMNA 编码的蛋白质[1][2],属于核纤层蛋白家族。 ... 2000, 177 (1): 1-11. PMID 10960149. doi:10.1007/s002320001096.. *. Burke B, Mounkes LC, Stewart CL. The nuclear envelope in ... 2007, 53 (1): 46-52. PMID 17718387.. *. Genschel J, Schmidt HH. Mutations in the LMNA gene encoding lamin A/C. Hum. Mutat. ... Type 1/2
62 (1): 121-3. doi:10.1292/jvms.62.121. PMID 10676904.. *. Chakarova C, Wehnert MS, Uhl K, et al. (2001). "Genomic structure ... Shoeman RL, Hartig R, Hauses C, Traub P (2003). "Organization of focal adhesion plaques is disrupted by action of the HIV-1 ...
... was once thought to bind cofilin but is now believed to enhance the interactions between the V domains and actin monomers, as ... doi:10.1016/S0092-8674(00)80732-1. PMID 10219243.. *^ Thrasher AJ, Burns SO (March 2010). "WASP: a key immunological ... 120 (1): 2-9. doi:10.1046/j.1365-2249.2000.01193.x. PMC 1905602. PMID 10759756.. ...
67 (1): 37-46. doi:10.1086/302962. PMC 1287099. PMID 10841809.. *. De Jonghe P, Mersivanova I, Nelis E, Del Favero J, Martin JJ ... 45 (1): 30-2. doi:10.1159/000132421. PMID 3036423.. *. Frappier T, Regnouf F, Pradel LA (December 1987). "Binding of brain ... Pospelov VA, Pospelova TV, Julien JP (February 1994). "AP-1 and Krox-24 transcription factors activate the neurofilament light ... 10.1083/jcb.143.1.1. PMC 2132816. PMID 9763415.. *. Beaudet L, Charron G, Julien JP (May 1992). "Origin of the two mRNA species ...
Elastic filaments, 1 nm in diameter, are made of titin, a large springy protein. They run through the core of each thick ... Myofilaments are the filaments of myofibrils, constructed from proteins,[1] principally myosin or actin. Types of muscle are ...
Interaction of the alpha subunit of Na,K-ATPase with cofilin by K. Lee, J. Jung, M. Kim and G. Guidotti in The Biochemical ... 4.1/2/3/4/5/6 · 5.1/2/3/4/5/99 · 6.1-3(英语:Template:Ligases CO CS and CN)/4/5-6 ... 1]. 蛋白質交互作用對鈉鉀幫浦媒介的訊息傳遞是很重要的,如:鈉鉀幫浦與無接收子酪胺酸磷酸酶(Src,一種沒有受體得酪胺酸激酶)結合形成接收
26 (1): 103-5. doi:10.1038/79063. PMID 10973259.. *^ a b c d Seri M, Pecci A, Di Bari F, Cusano R, Savino M, Panza E, et al. ( ... 37 (1): 112-20. doi:10.1097/AUD.0000000000000198. PMID 26226608.. *^ a b Kunishima S, Matsushita T, Kojima T, Sako M, Kimura F ... 26 (1): 106-8. doi:10.1038/79069. PMID 10973260.. *^ Seri M, Cusano R, Gangarossa S, Caridi G, Bordo D, Lo Nigro C, et al. ( ... 142 (1): 221-30. PMC 1886840. PMID 8424456.. *. Lalwani AK, Linthicum FH, Wilcox ER, Moore JK, Walters FC, San Agustin TB, ...
Talin-1 is a protein that in humans is encoded by the TLN1 gene.[5][6] Talin-1 is ubiquitously expressed, and is localized to ... Human talin-1 is 270.0 kDa molecular weight and 2541 amino acids.[7] The N-terminal region of talin-1 is ~50 kDa in size and ... Talin-1 functions to mediate cell-cell adhesion via the linkage of integrins to the actin cytoskeleton and in the activation of ... In mammals talin-1 is ubiquitously expressed; talin-1 is found complexed to integrins and localized to intercalated discs of ...
11 (1): 45-53. doi:10.1016/S0955-0674(99)80006-4.. *^ Moughamian, Armen J.; Osborn, Gregory E.; Lazarus, Jacob E.; Maday, ... ISBN 0-471-19279-1.. *. Schroer TA (2004). "Dynactin". Annual Review of Cell and Developmental Biology. 20: 759-79. doi:10.1146 ... Cytoplasmic dynein helps to position the Golgi complex and other organelles in the cell.[1] It also helps transport cargo ... 1] Cytoplasmic dynein moves processively along the microtubule; that is, one or the other of its stalks is always attached to ...
"Interaction of the alpha subunit of Na,K-ATPase with cofilin". The Biochemical Journal. 353 (Pt 2): 377-85. doi:10.1042/0264- ... 978-0-470-57095-1. .. *^ Howarth C, Gleeson P, Attwell D (July 2012). "Updated energy budgets for neural computation in the ... Each has unique properties and tissue expression patterns.[1] This enzyme belongs to the family of P-type ATPases. ... 252 (3 Pt 1): C328-34. doi:10.1152/ajpcell.1987.252.3.c328. PMID 3030131.. ...
Ankyrin-B, also known as Ankyrin-2, is a protein which in humans is encoded by the ANK2 gene.[1][2] Ankyrin-B is ubiquitously ... 1 (2): 93-9. doi:10.1161/circgenetics.108.792192. PMID 20031550.. *^ Alders, M; Christiaans, I; Pagon, RA; Adam, MP; Ardinger, ... ankyrin-1 has shown to be essential in normal function of erythrocytes;[10] however, ankyrin-B and ankyrin-3 play essential ... Mohler, PJ; Davis, JQ; Davis, LH; Hoffman, JA; Michaely, P; Bennett, V (26 March 2004). "Inositol 1,4,5-trisphosphate receptor ...
... , HB1, Hb-1, KRTHB1, MLN137, ghHkb1, hHAKB2-1, keratin 81. External IDs. OMIM: 602153 MGI: 1928858 HomoloGene: 55645 ... 2003). "Upregulation of the truncated basic hair keratin 1(hHb1-DeltaN) in carcinoma cells by Epstein-Barr virus (EBV)". Int. J ...
... s (/ˈmaɪəsɪn, -oʊ-/[1][2]) are a superfamily of motor proteins best known for their roles in muscle contraction and in a ... 51 (1): 1-15. doi:10.1002/cm.10014. PMID 11810692.. *^ von der Ecken J, Heissler SM, Pathan-Chhatbar S, Manstein DJ, Raunser S ... 46 (1): 61-7. doi:10.1002/ajmg.1320460110. PMID 7684189.. *^ "Archived copy". Archived from the original on 2009-07-07. ... 13 (1): 13-26. doi:10.1038/nrm3248. PMID 22146746. S2CID 11853457.. *^ Zimmermann D, Santos A, Kovar DR, Rock RS (August 2015 ...
CK-1. CK-2. CK-3. CK-4. CK-5. CK-6. CK-9. CK-10. CK-11. CK-12. CK-13. CK-14. CK-15. CK-16. CK-17 ... They are an important component of intermediate filaments, which help cells resist mechanical stress.[1] Expression of these ...
Cofilin-actin complex finally dissociate after cofilin phosphorylation by nuclear LIM kinase. ... The most notable of these proteins are gelsolin and cofilin. These proteins first achieve a cut by binding to an actin monomer ... The import of actin into the nucleus (probably in a complex with cofilin) is facilitated by the import protein importin 9.[96] ... They possess a number of accessory proteins including ADF/cofilin, which has a molecular weight of 16kDa and is coded for by a ...
... cofilin. ADP bound cofilin severs ADP-rich regions nearest the (−)-ends. Upon release, the free actin monomer slowly ... Actin depolymerizing proteins such as ADF/cofilin.. The actin filament network in non-muscle cells is highly dynamic. The actin ... cell signalling is believed to activate cofilin, the actin-filament depolymerizing protein which binds to ADP-rich actin ... ISBN 1-4160-2388-7.. *^ Galland R, Leduc P, Guérin C, Peyrade D, Blanchoin L, Théry M (2013). "Fabrication of three-dimensional ...
... was first described in 1976,[16] first purified in 1977,[17] the gene was cloned in 1989,[6] and the first knockout mouse was created in 1996.[18] The function of desmin has been deduced through studies in knockout mice. Desmin is one of the earliest protein markers for muscle tissue in embryogenesis as it is detected in the somites.[12] Although it is present early in the development of muscle cells, it is only expressed at low levels, and increases as the cell nears terminal differentiation. A similar protein, vimentin, is present in higher amounts during embryogenesis while desmin is present in higher amounts after differentiation. This suggests that there may be some interaction between the two in determining muscle cell differentiation. However desmin knockout mice develop normally and only experience defects later in life.[13] Since desmin is expressed at a low level during differentiation another protein may be able to compensate for desmin's function early in development but not ...
582 (1): 1-13. doi:10.1016/J.GENE.2016.01.006. PMC 5325693. PMID 26774798.. CS1 maint: Uses authors parameter (link). .mw- ... 1). Sequence homology and protein epitope allosteric similarity data suggest that TnT gene was originated by duplication of a ... 276 (5 Pt 1): C1162-70. doi:10.1152/ajpcell.1999.276.5.C1162. PMID 10329966.. ... 143 (1): 47-56. doi:10.1111/j.1432-1033.1984.tb08337.x. PMID 6468390.. ...
Cai L, Marshall TW, Uetrecht AC, Schafer DA, Bear JE (May 2007). "Coronin 1B coordinates Arp2/3 complex and cofilin activities ... Whereas coronin 1 was required for chemokine-mediated migration, it was dispensable for T cell antigen receptor functions in T ... 107 (1): 227-39. PMID 8175911. Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of ... N-terminus signature region is reduced to 5aa and appears in front of each WD-repeat core domain (e.g., CRN7, POD-1) Human ...
When the nascent endosome forms, the recruitment of cofilin by ClC-5 is a prerequisite for the localized dissolution of the ... Hryciw DH, Wang Y, Devuyst O, Pollock CA, Poronnik P, Guggino WB (October 2003). "Cofilin interacts with ClC-5 and regulates ... Hryciw DH, Wang Y, Devuyst O, Pollock CA, Poronnik P, Guggino WB (October 2003). "Cofilin interacts with ClC-5 and regulates ... It was demonstrated at the C-terminus of ClC-5 binds the actin-depolymerizing protein cofilin. ...
ADF/cofilins, which include ADF, cofilin, destrin, actophorin, coactosin, depactin and glia maturation factors (GMFs) beta and ... ADF/cofilins are small actin-binding proteins composed of a single ADF-H domain. They bind both actin-monomers and filaments ... ADF/cofilins bind ADP-actin with higher affinity than ATP-actin and inhibit the spontaneous nucleotide exchange on actin ... cofilin". J. Biol. Chem. 277 (45): 43089-95. doi:10.1074/jbc.M208225200. PMID 12207032. Liu LX, Xu H, Weller PF, Shi A, Debnath ...
Spine heads are enlarged due to the increased phosphorylation of cofilin, leading to a decrease in F-actin severing and ... 321 (1): 210-8. doi:10.1016/j.bbrc.2004.06.122. PMID 15358237. Rumbaugh G, Adams JP, Kim JH, Huganir RL (March 2006). "SynGAP ... 1005 (1-2): 44-50. doi:10.1016/j.brainres.2004.01.032. PMID 15044063. S2CID 33854259. Brandenberger R, Wei H, Zhang S, Lei S, ... 21 (12): 1-13. doi:10.1038/s41593-018-0268-0. ISSN 1546-1726. PMC 6309426. PMID 30455457. Vlaskamp DR, Shaw BJ, Burgess R, Mei ...
Madak-Erdogan, Zeynep; Ventrella, Rosa; Petry, Luke; Katzenellenbogen, Benita S (2014). "Novel Roles for ERK5 and Cofilin as ... 92 (1): 125-34. doi:10.1210/endo-92-1-125. PMID 4681918. "People , Carl R. Woese Institute for Genomic Biology". www.igb. ... 31 (1): 226-36. doi:10.1128/MCB.00821-10. PMC 3019850. PMID 20956553. Harrington, William R; Kim, Sung Hoon; Funk, Cory C; ... 105 (1): 33-40. doi:10.1210/endo-105-1-33. PMID 446414. Gong, Ping; Madak-Erdogan, Zeynep; Li, Jilong; Cheng, Jianlin; ...
LIM-kinase will in turn phosphorylate and inactivate cofilin, which will prevent the breakdown and recycling of actin filaments ... 28 (1-2): 65-76. doi:10.1007/s10555-008-9170-7. PMID 19160018. S2CID 33869424. Kimura, K.; Ito, M.; Amano, M.; Chihara, K.; ... 6 (1): 21-30. doi:10.1038/ncb1075. PMC 2605950. PMID 14647292. Maul, R. S. (February 3, 2003). "EPLIN regulates actin dynamics ... 6 (1): 275-302. doi:10.1146/annurev.bioeng.6.040803.140040. PMID 15255771. Chen, Christopher S.; Tan, John; Tien, Joe (August ...

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  • Mitochondrial fission and mitophagy depend on cofilin-mediated actin depolymerization activity at the mitochondrial fission site. (cocites.com)
  • Shashank's paper on Srv2/CAP and Cofilin synergizing in ultrafast actin depolymerization has been published at Nature Communications. (goodelab.org)
  • This improved 51059-44-0 manufacture insulin discharge resulted from an increased pancreatic parasympathetic insight on islets and from an increased awareness of -cells to cholinergic and GLP-1 arousal. (molecularcircuit.com)
  • Auditory brainstem-evoked response testing, immunofluorescence imaging, scanning electron microscopy, and biochemical labeling techniques were used to study how the ADF/cofilin family of actin filament severing and depolymerizing proteins contributes to the development of the stereocilia bundle. (purdue.edu)
  • A novel role of differentiation-inducing factor-1 in Dictyostelium development, assessed by the restoration of a developmental defect in a mutant lacking mitogen-activated protein kinase ERK2. (nbrp.jp)
  • Cytoskeletal regulators, such as for example Rho GTPases 51059-44-0 manufacture or cofilin, mediate the axonal and neurite development inhibitory actions of Nogo-A (28,29). (molecularcircuit.com)
  • The activity of this channel regulates ADF/cofilins and the actin at stereocilia tips. (purdue.edu)
  • Genomic architecture and prediction of censored time-to-event phenotypes with a Bayesian genome-wide analysis," Nature Communications , vol. 12, no. 1. (ist.ac.at)
  • J. L. Fischer and D. Matthes, "The waiting time phenomenon in spatially discretized porous medium and thin film equations," SIAM Journal on Numerical Analysis , vol. 59, no. 1. (ist.ac.at)

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