Cofilin 1 is a member of the cofilin family of proteins that is expressed in non-muscle CELLS. It has ACTIN depolymerization activity that is dependent on HYDROGEN-ION CONCENTRATION.
A family of low MOLECULAR WEIGHT actin-binding proteins found throughout eukaryotes. They remodel the actin CYTOSKELETON by severing ACTIN FILAMENTS and increasing the rate of monomer dissociation.
A member of the actin depolymerizing factors. Its depolymerizing activity is independent of HYDROGEN-ION CONCENTRATION.
Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)
A member of the cofilin family of proteins that is expressed in MUSCLE CELLS. It has ACTIN depolymerization activity that is dependent on HYDROGEN-ION CONCENTRATION.
Filamentous proteins that are the main constituent of the thin filaments of muscle fibers. The filaments (known also as filamentous or F-actin) can be dissociated into their globular subunits; each subunit is composed of a single polypeptide 375 amino acids long. This is known as globular or G-actin. In conjunction with MYOSINS, actin is responsible for the contraction and relaxation of muscle.
Serine protein kinases involved in the regulation of ACTIN polymerization and MICROTUBULE disassembly. Their activity is regulated by phosphorylation of a threonine residue within the activation loop by intracellular signaling kinases such as P21-ACTIVATED KINASES and by RHO KINASE.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.
Immunoglobulins produced in response to VIRAL ANTIGENS.
Antibodies produced by a single clone of cells.
Any of numerous agile, hollow-horned RUMINANTS of the genus Capra, in the family Bovidae, closely related to the SHEEP.
The species Oryctolagus cuniculus, in the family Leporidae, order LAGOMORPHA. Rabbits are born in burrows, furless, and with eyes and ears closed. In contrast with HARES, rabbits have 22 chromosome pairs.
An amorphous region of electron dense material in the cytoplasm from which the MICROTUBULES polymerization is nucleated. The pericentriolar region of the CENTROSOME which surrounds the CENTRIOLES is an example.
Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.
Protein factors that promote the exchange of GTP for GDP bound to GTP-BINDING PROTEINS.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Experimentation on STEM CELLS and on the use of stem cells.
The interfaces between T-CELLS and ANTIGEN-PRESENTING CELLS. Supramolecular organization of proteins takes place at these synapses involving various types of immune cells. Immunological synapses can have several functions including LYMPHOCYTE ACTIVATION; enhancing, balancing, or terminating signaling; or directing cytokine secretion.
An organized procedure carried out by a select committee of professionals in evaluating the performance of other professionals in meeting the standards of their specialty. Review by peers is used by editors in the evaluation of articles and other papers submitted for publication. Peer review is used also in the evaluation of grant applications. It is applied also in evaluating the quality of health care provided to patients.
Lymphoid cells concerned with humoral immunity. They are short-lived cells resembling bursa-derived lymphocytes of birds in their production of immunoglobulin upon appropriate stimulation.
An actin capping protein that binds to the pointed-end of ACTIN. It functions in the presence of TROPOMYOSIN to inhibit microfilament elongation.
A 90-kDa protein produced by macrophages that severs ACTIN filaments and forms a cap on the newly exposed filament end. Gelsolin is activated by CALCIUM ions and participates in the assembly and disassembly of actin, thereby increasing the motility of some CELLS.
A protein found in the thin filaments of muscle fibers. It inhibits contraction of the muscle unless its position is modified by TROPONIN.
Monomeric subunits of primarily globular ACTIN and found in the cytoplasmic matrix of almost all cells. They are often associated with microtubules and may play a role in cytoskeletal function and/or mediate movement of the cell or the organelles within the cell.
Fibers composed of MICROFILAMENT PROTEINS, which are predominately ACTIN. They are the smallest of the cytoskeletal filaments.
A high molecular weight (220-250 kDa) water-soluble protein which can be extracted from erythrocyte ghosts in low ionic strength buffers. The protein contains no lipids or carbohydrates, is the predominant species of peripheral erythrocyte membrane proteins, and exists as a fibrous coating on the inner, cytoplasmic surface of the membrane.
Databases containing information about PROTEINS such as AMINO ACID SEQUENCE; PROTEIN CONFORMATION; and other properties.
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.
The portion of an interactive computer program that issues messages to and receives commands from a user.
Sequential operating programs and data which instruct the functioning of a digital computer.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A process that includes the determination of AMINO ACID SEQUENCE of a protein (or peptide, oligopeptide or peptide fragment) and the information analysis of the sequence.
Neoplasms composed of cells from the deepest layer of the epidermis. The concept does not refer to neoplasms located in the stratum basale.
A smooth, solid or cystic fibroepithelial (FIBROEPITHELIAL NEOPLASMS) tumor, usually found in the OVARIES but can also be found in the adnexal region and the KIDNEYS. It consists of a fibrous stroma with nests of epithelial cells that sometimes resemble the transitional cells lining the urinary bladder. Brenner tumors generally are benign and asymptomatic. Malignant Brenner tumors have been reported.
Tumors or cancer of the human BREAST.
A cell line derived from cultured tumor cells.
In humans, one of the paired regions in the anterior portion of the THORAX. The breasts consist of the MAMMARY GLANDS, the SKIN, the MUSCLES, the ADIPOSE TISSUE, and the CONNECTIVE TISSUES.
The transfer of a neoplasm from one organ or part of the body to another remote from the primary site.
The network of filaments, tubules, and interconnecting filamentous bridges which give shape, structure, and organization to the cytoplasm.
The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.

The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics. (1/145)

Actin-depolymerizing factor (ADF)/cofilins are essential regulators of actin filament turnover. Several ADF/cofilin isoforms are found in multicellular organisms, but their biological differences have remained unclear. Herein, we show that three ADF/cofilins exist in mouse and most likely in all other mammalian species. Northern blot and in situ hybridization analyses demonstrate that cofilin-1 is expressed in most cell types of embryos and adult mice. Cofilin-2 is expressed in muscle cells and ADF is restricted to epithelia and endothelia. Although the three mouse ADF/cofilins do not show actin isoform specificity, they all depolymerize platelet actin filaments more efficiently than muscle actin. Furthermore, these ADF/cofilins are biochemically different. The epithelial-specific ADF is the most efficient in turning over actin filaments and promotes a stronger pH-dependent actin filament disassembly than the two other isoforms. The muscle-specific cofilin-2 has a weaker actin filament depolymerization activity and displays a 5-10-fold higher affinity for ATP-actin monomers than cofilin-1 and ADF. In steady-state assays, cofilin-2 also promotes filament assembly rather than disassembly. Taken together, these data suggest that the three biochemically distinct mammalian ADF/cofilin isoforms evolved to fulfill specific requirements for actin filament dynamics in different cell types.  (+info)

The human D2 dopamine receptor synergizes with the A2A adenosine receptor to stimulate adenylyl cyclase in PC12 cells. (2/145)

The adenosine A(2A) receptor and the dopamine D(2) receptor are prototypically coupled to G(s) and G(i)/G(o), respectively. In striatal intermediate spiny neurons, these receptors are colocalized in dendritic spines and act as mutual antagonists. This antagonism has been proposed to occur at the level of the receptors or of receptor-G protein coupling. We tested this model in PC12 cells which endogenously express A(2A) receptors. The human D(2) receptor was introduced into PC12 cells by stable transfection. A(2A)-agonist-mediated inhibition of D(2) agonist binding was absent in PC12 cell membranes but present in HEK293 cells transfected as a control. However, in the resulting PC12 cell lines, the action of the D(2) agonist quinpirole depended on the expression level of the D(2) receptor: at low and high receptor levels, the A(2A)-agonist-induced elevation of cAMP was enhanced and inhibited, respectively. Forskolin-stimulated cAMP formation was invariably inhibited by quinpirole. The effects of quinpirole were abolished by pretreatment with pertussis toxin. A(2A)-receptor-mediated cAMP formation was inhibited by other G(i)/G(o)-coupled receptors that were either endogenously present (P(2y12)-like receptor for ADP) or stably expressed after transfection (A(1) adenosine, metabotropic glutamate receptor-7A). Similarly, voltage activated Ca(2+) channels were inhibited by the endogenous P(2Y) receptor and by the heterologously expressed A(1) receptor but not by the D(2) receptor. These data indicate functional segregation of signaling components. Our observations are thus compatible with the proposed model that D(2) and A(2A) receptors are closely associated, but they highlight the fact that this interaction can also support synergism.  (+info)

Actin-depolymerizing factor and cofilin-1 play overlapping roles in promoting rapid F-actin depolymerization in mammalian nonmuscle cells. (3/145)

Actin-depolymerizing factor (ADF)/cofilins are small actin-binding proteins found in all eukaryotes. In vitro, ADF/cofilins promote actin dynamics by depolymerizing and severing actin filaments. However, whether ADF/cofilins contribute to actin dynamics in cells by disassembling "old" actin filaments or by promoting actin filament assembly through their severing activity is a matter of controversy. Analysis of mammalian ADF/cofilins is further complicated by the presence of multiple isoforms, which may contribute to actin dynamics by different mechanisms. We show that two isoforms, ADF and cofilin-1, are expressed in mouse NIH 3T3, B16F1, and Neuro 2A cells. Depleting cofilin-1 and/or ADF by siRNA leads to an accumulation of F-actin and to an increase in cell size. Cofilin-1 and ADF seem to play overlapping roles in cells, because the knockdown phenotype of either protein could be rescued by overexpression of the other one. Cofilin-1 and ADF knockdown cells also had defects in cell motility and cytokinesis, and these defects were most pronounced when both ADF and cofilin-1 were depleted. Fluorescence recovery after photobleaching analysis and studies with an actin monomer-sequestering drug, latrunculin-A, demonstrated that these phenotypes arose from diminished actin filament depolymerization rates. These data suggest that mammalian ADF and cofilin-1 promote cytoskeletal dynamics by depolymerizing actin filaments and that this activity is critical for several processes such as cytokinesis and cell motility.  (+info)

Maternal diet programs embryonic kidney gene expression. (4/145)

Human epidemiological data associating birth weight with adult disease suggest that organogenesis is "programmed" by maternal diet. In rats, protein restriction in pregnancy produces offspring with fewer renal glomeruli and higher systemic blood pressures than controls. We tested the hypothesis that maternal diet alters gene expression in the metanephros, the precursor of the definitive mammalian kidney. We demonstrated that maternal low-protein diet initiated when pregnancy starts and maintained to embryonic day 13, when the metanephros consists of mesenchyme surrounding a once-branched ureteric bud, is sufficient to significantly reduce glomerular numbers in offspring by about 20%. As assessed by representational difference analyses and real-time quantitative polymerase chain reactions, low-protein diet modulated gene expression in embryonic day 13 metanephroi. In particular, levels of prox-1, the ortholog of Drosophila transcription factor prospero, and cofilin-1, a regulator of the actin cytoskeleton, were reduced. During normal metanephrogenesis, prox-1 protein was first detected in mesenchymal cells around the ureteric tree and thereafter in nascent nephron epithelia, whereas cofilin-1 immunolocalized to bud derivatives and condensing mesenchyme. Previously, we reported that low-protein diets increased mesenchymal apoptosis cells when metanephrogenesis began and thereafter reduced numbers of precursor cells. Collectively, these studies prove that the maternal diet programs the embryonic kidney, altering cell turnover and gene expression at a time when nephrons and glomeruli have yet to form. The human implication is that the maternal diet ingested between conception and 5- 6-wk gestation contributes to the variation in glomerular numbers that are known to occur between healthy and hypertensive populations.  (+info)

Micropuncture gene delivery and intravital two-photon visualization of protein expression in rat kidney. (5/145)

Understanding molecular mechanisms of pathophysiology and disease processes requires the development of new methods for studying proteins in animal tissues and organs. Here, we describe a method for adenoviral-mediated gene transfer into tubule or endothelial cells of the rat kidney. The left kidney of an anesthetized rat was exposed and the lumens of superficial proximal tubules or vascular welling points were microinfused, usually for 20 min. The microinfusion solution contained adenovirus with a cDNA construct of either 1) Xenopus laevis actin depolymerizing factor/cofilin [XAC; wt-green fluorescent protein (GFP)], 2) actin-GFP, or 3) GFP. Sudan black-stained castor oil, injected into nearby tubules, allowed us to localize the microinfused structures for subsequent visualization. Two days later, the rat was anesthetized and the kidneys were fixed for tissue imaging or the left kidney was observed in vivo using two-photon microscopy. Expression of GFP and GFP-chimeric proteins was clearly seen in epithelial cells of the injected proximal tubules and the expressed proteins were localized similarly to their endogenously expressed counterparts. Only a minority of the cells in the virally exposed regions, however, expressed these proteins. Endothelial cells also expressed XAC-GFP after injection of the virus cDNA construct into vascular welling points. An advantage of the proximal tubule and vascular micropuncture approaches is that only minute amounts of virus are required to achieve protein expression in vivo. This micropuncture approach to gene transfer of the virus cDNA construct and intravital two-photon microscopy should be applicable to study of the behavior of any fluorescently tagged protein in the kidney and shows promise in studying renal physiology and pathophysiology.  (+info)

Ligation of cell surface-associated glucose-regulated protein 78 by receptor-recognized forms of alpha 2-macroglobulin: activation of p21-activated protein kinase-2-dependent signaling in murine peritoneal macrophages. (6/145)

Previous studies of the plasma proteinase inhibitor alpha2-macroglobulin (alpha2M) demonstrated that alpha2M-proteinase complexes (alpha2M*) modulate immune responses and promotes macrophage locomotion and chemotaxis. Alpha2M* binds to cell surface-associated glucose-regulated protein 78 (GRP78), which activates downstream signaling events. The role of p21-activated protein kinase-1 and -2 (PAK-1 and -2) in promoting cellular motility is well documented. In the current study, we examined the ability of alpha2M* to activate PAK-1 and PAK-2. Upon macrophage stimulation with alpha2M*, PAK-2 is autophosphorylated, resulting in increased kinase activity; however, PAK-1 is negligibly affected. Alpha2M*-stimulated macrophages showed a marked elevation in the levels of Rac x GTP. Receptor tyrosine phosphorylation upon binding of alpha2M* to GRP78, recruits PAK-2 to the plasma membrane via the adaptor protein NCK. Consistent with this hypothesis, silencing of GRP78 gene expression greatly attenuated the levels of membrane-associated PAK-2 and NCK. PAK-2 activity was markedly decreased by inhibition of tyrosine kinases and PI3K before alpha2M* stimulation. We further demonstrate that phosphorylation of Lin-11, Isl-1, Mec-3 (LIM) kinase and cofilin is promoted by treating macrophages with alpha2M*. Thus, alpha2M* regulates activation of the PAK-2-dependent motility mechanism in these cells.  (+info)

Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5' overhangs with run through stop DNA synthesis utilizing Ara-C. (7/145)

BACKGROUND: Splicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches. RESULTS: A method for DNA splicing and mutagenesis without restriction enzymes is described. The method is based on mild template-dependent polymerization arrest with two molecules of cytosine arabinose (Ara-C) incorporated into PCR primers. Two rounds of PCR are employed: the first PCR produces 5' overhangs that are utilized for DNA splicing. The second PCR is based on polymerization running through the Ara-C molecules to produce the desired final product. To illustrate application of the run through stop mutagenesis and DNA splicing technique, we have carried out splicing of two segments of the human cofilin 1 gene and introduced a mutational deletion into the product. CONCLUSION: We have demonstrated the utility of a new PCR-based method for carrying out DNA splicing and mutagenesis by incorporating Ara-C into the PCR primers.  (+info)

Stimulation of actin polymerization by filament severing. (8/145)

The extent and dynamics of actin polymerization in solution are calculated as functions of the filament severing rate, using a simple model of in vitro polymerization. The model is solved by both analytic theory and stochastic-growth simulation. The results show that severing essentially always enhances actin polymerization by freeing up barbed ends, if barbed-end cappers are present. Severing has much weaker effects if only pointed-end cappers are present. In the early stages of polymerization, the polymerized-actin concentration grows exponentially as a function of time. The exponential growth rate is given in terms of the severing rate, and the latter is given in terms of the maximum slope in a polymerization time course. Severing and branching are found to act synergistically.  (+info)

DNA constructs, reagents, and antibodies. Full-length human SSH1L cDNA was amplified by PCR using the RZPD clone IRAUp969F0865D6 as a template and cloned into pCR3.V62-Met-Flag and pEGFP-N3 vectors. The SSH1L point mutant was generated by site-directed PCR mutagenesis. Integrity of all constructs was verified by sequencing. Antibodies used were as follows: anti-PKD2 rabbit polyclonal antibody (Calbiochem), anti-PKD C-20 (Santa Cruz Biotechnology), anti-phospho-cofilin (Ser3) and anti-cofilin rabbit polyclonal antibodies (Cell Signaling), anti-Flag M2 mouse monoclonal antibody (Sigma-Aldrich, Germany), anti-tubulin mouse monoclonal antibody (Neomarkers), anti-SSH1L rabbit polyconal antibody (Abcam), and anti-glutathione S-transferase (GST) goat polyclonal antibody (GE Healthcare). Secondary antibodies used were horseradish peroxidase-coupled donkey anti-goat, goat anti-mouse, and anti-rabbit IgGs (Dianova). Alexa546- and Alexa633-coupled phalloidin were from Invitrogen.. Cell culture. Cells were ...
During early metastatic events in cancer, reorganization of the actin cytoskeleton must occur to allow for cell invasion and migration. A key enzyme initiating actin remodeling at the leading edge is Cofilin, a small actin binding protein of the ADF/cofilin family with actin severing activities. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation events that are mediated by LIM Kinase (LIMK) and the phosphatase Slingshot (SSH), respectively. Protein kinase D (PKD) is a serine/threonine kinase that has previously been shown to regulate actin driven directed cell migration through phosphorylation and thus inactivation of Slingshot. Here we show that PKD can also regulate cofilin activity via activation of LIMK. We further show that PKD-induced regulation of LIMK and the resulting diminished cofilin activity translates to altered cell motility. Our data suggest that PKD inactivates Cofilin through modulation of both of its regulatory pathways. Further elucidation of the ...
Cofilin 1 (non-muscle; n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus. One group reports that reelin signaling leads to serine3-phosphorylation of cofilin-1, and this interaction may play a role in the reelin-related regulation of neuronal migration. Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000056201 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Entrez Gene: CFL1 cofilin 1 (non-muscle). Chai X, Förster E, Zhao S, Bock HH, Frotscher M (January 2009). Reelin stabilizes the actin cytoskeleton of neuronal processes by inducing n-cofilin ...
The actin depolymerizing factor (ADF)/cofilin protein family is essential for actin dynamics, cell division, chemotaxis and tumor metastasis. Cofilin-1 (CFL-1) is a primary non-muscle isoform of the ADF/cofilin protein family accelerating the actin filamental turnover in vitro and in vivo. In response to environmental stimulation, CFL-1 enters the nucleus to regulate the actin dynamics. Although the purpose of this cytoplasm-nucleus transition remains unclear, it is speculated that the interaction between CFL-1 and DNA may influence various biological responses, including DNA damage repair. In this review, we will discuss the possible involvement of CFL-1 in DNA damage responses (DDR) induced by ionizing radiation (IR), and the implications for cancer radiotherapy.
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A) PirB interacts with cofilin in vivo in PirB+/− Tg mice (P30, forebrain), assessed by immunoprecipitation for PirB. Other known PirB-proximal signaling and interactions such as tyrosine phosphorylation of PirB and SHP-2 recruitment to PirB are not altered in PirB+/− relative to PirB+/− Tg mice. Representative data are shown (n , 2 ...
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Cofilin 2 antibody (cofilin 2 (muscle)) for WB. Anti-Cofilin 2 pAb (GTX14133) is tested in Human, Mouse, Rat samples. 100% Ab-Assurance.
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MAPK continues to be proven to limit activation of MAPK 経路 LIMK, subsequent phosphorylation of cofilin, and migration of major human T cells in the three dimen
Having an existing gmfgen file and a generated diagram plug-in, but trying to generate the same diagram plug-in results in different java classes structure ...
Having an existing gmfgen file and a generated diagram plug-in, but trying to generate the same diagram plug-in results in different java classes structure ...
The IS is a polarized cell structure similar to the leading edge of a migrating cell or the yeast bud site. Establishing functional patterns of membrane protein organization and vesicular traffic at these sites requires coordinated reorganization of the actin and microtubule cytoskeletons. Rap1 is an evolutionarily conserved regulator of cell polarity that drives actin reorganization at the B cell IS (Lin et al., 2008). We have now shown that Rap1, and its downstream target, the actin-severing protein cofilin, are essential for BCR-induced MTOC polarization towards the IS. Whether Rap1 controls MTOC reorientation in other immune cells is not known. The BCR and TCR both activate Rap1 via phospholipase Cγ-dependent production of diacylglycerol (Katagiri et al., 2004; McLeod et al., 1998), which recruits the Rap1 exchange factor RasGRP2 (also known as CalDAG-GEFI) (Kawasaki et al., 1998) to the plasma membrane. The scaffolding protein ADAP (also known as FYB), which promotes the accumulation or ...
To make Angry Birds a real sport, Mbed made a USB slingshot to play Angry Birds. The slingshot emulates a USB mouse, so it really is a plug n play. It translates the physical use of the slingshot in to appropriate mouse controls. The way the controller works is reasonably simple: a stretch sensor translates the drawing of the slingshot to a mouse movement and an accelerometer determines the angle. Check out the video of it in action. ...
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Alzheimers disease (AD) is a neurodegenerative disorder characterized by two major pathological hallmarks, amyloid plaques and neurofibrillary tangles. The accumulation of amyloid-β protein (Aβ) is an early event associated with synaptic and mitochondrial damage in AD. Therefore, molecular pathways underlying the neurotoxicity and generation of Aβ represent promising therapeutic targets for AD. Recent studies have shown that actin severing protein, Cofilin plays an important role in synaptic remodeling, mitochondrial dysfunction, and AD pathogenesis. However, whether Cofilin is an essential component of AD pathogenesis and how Aβ induced neurotoxicity impinges its signals to Cofilin are unclear. In my dissertation studies, we found Aβ oligomers bind with intermediate activation conformers of β1-integrin to induce the loss of surface β1-integrin and activation of Cofilin via Slingshot homology-1 (SSH1) activation. Specifically, conditional loss of β1-integrin prevented Aβ induced Cofilin
Cofilin belongs to the actin-binding ADF protein family. It contains one ADF-H domain. Cofilin controls reversible actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin at a 1:1 ratio. It is the major component of both intranuclear and cytop...
Real‐time imaging. Cells were seeded in a Lab‐Tek Chambered #1.0 Borosilicate Coverglass System (Nalgene Nunc International, Rochester, NY, USA) and were mounted on a Zeiss Axiovert 200M microscope for live‐cell imaging (5% CO2; 37°C) for 2-14 h. Phase‐contrast images were captured every 2 min using a Photometics Coolsnap CCD camera (Scientific, Tuscon, AZ, USA). Images were processed using the Metamorph software (Universal imaging, Downington, PA, USA). Several protrusions was scored by off‐line analysis of the generated videos and plotted as means±s.e.m. For real‐time fluorescence imaging, cells were placed on a Leica SP5 inverted microscope equipped with a × 63 1.3NA glycerol objective. Cells were maintained in Leibovitz‐15‐buffered medium (Invitrogen, Breda, The Netherlands) at 37°C in a climate chamber.. Invasion assay. For in vitro invasion assays, 24‐well BioCoat matrigel invasion chambers (#354480, BD Biosciences, Alphen aan den Rijn, The Netherlands) were used ...
PAK4_HUMAN] Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of ...
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Actin-severing proteins ADF/cofilin are required for the sorting of secretory cargo at the trans-Golgi network (TGN) in mammalian cells. How do these cytoplasmic proteins interact with the cargoes in the lumen of the TGN ...
Reporting on the project, Dr Goldsbury wrote, In this project supported by the SZCUF, we have demonstrated that reduced energy metabolism by inhibition of mitochondrial function in cultured neurons gives rise to the redistribution of tau and cofilin into rod-like structures that appear to mimic those in the AD brain. Further, we demonstrated that the density of cofilin rods in human AD brains is significantly higher than in normal aged human brain. However, we also showed that some aged normal brains contained low levels of cofilin rods, although these brains were devoid of abnormal tau. This suggests that cofilin rod generation may be an early event in the neurodegenerative cascade representing a possible early-stage mechanism in the progression to AD dementia and therefore an ideal future target for therapeutic intervention that might be useful in treatment of neurological diseases. Unexpectedly, however, despite an extensive study, we were not able to show any substantial microscopic overlap ...
One candidate early on was a protein called slingshot, which seemed to have the correct phosphatase activity. However, slingshot turned out not to be found in all organisms, nor ubiquitously distributed throughout the body, a requirement for the mystery phosphatase, as it would be needed wherever actin dynamics is taking place. Additionally, slingshot did not appear to be capable of regulating all cofilin-dependent cell processes, so clearly a third man still existed.. Now in an article recently published in Nature Cell Biology, Bokoch and his colleagues Antje Gohla and Jörg Birkenfeld argue that the third man phosphatase is actually chronophin. Taking the increasingly overlooked approach of function-based biochemical isolation, which required Gohla to spend significant time in the cold room, the scientists purified a phosphatase activity which dephosphorylated cofilin. This activity was then identified by mass spectrometry as chronophin, a member of a unique superfamily of proteins called ...
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Three dimensional protein models of ADF/CFL proteins highlighting sites with accelerated rates of evolution (ω3 rate category) in pink.
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I would have gone, but I couldnt find a slingshot big enough. Oh, and I didnt realize there was a Cow Con III. But if it wasnt for those two things, I might have gone ...
The GAP first draft also has added the elements of NCDs Global Monitoring Framework (including twenty-five indicators) and Voluntary Global Targets (nine targets) finalised in the WHO meeting of member states late last year (5-7 November 2012). However the stakeholders want the 25 indicators of Global Monitoring Framework (GMF) and 9 voluntary global targets should be integrated in GAP alongwith evidence based interventions. Let us examine the GMF and GAP in some detail: ...
Ive updated this post from 2010 to include the correct link and new information! Also, please do not leave your address or account number in the comments below!
For the SSH-1 protocol, see Secure Shell#Version 1.x Protein phosphatase Slingshot homolog 1 is an enzyme that in humans is encoded by the SSH1 gene. The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002).[supplied by OMIM] GRCh38: Ensembl release 89: ENSG00000084112 - Ensembl, May 2017 GRCm38: Ensembl release 89: ENSMUSG00000042121 - Ensembl, May 2017 Human PubMed Reference:. Mouse PubMed Reference:. Nagase T, Kikuno R, Ishikawa KI, Hirosawa M, Ohara O (Apr 2000). Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 7 (1): 65-73. doi:10.1093/dnares/7.1.65. PMID 10718198. Niwa ...
Cofilin phospho-regulation is important for actin filament turnover and is implicated in cancer. Phosphorylation of cofilin is mediated by LIM kinases (LIMKs) and dephosphorylation by Slingshot phosphatases (SSH). LIMKs and SSH promote cancer cell invasion and metastasis and represent novel anti-cancer targets. However, little is known regarding LIMK/cofilin and SSH in human colorectal cancer (CRC). In this study, we aimed to address their expression and significance in human CRC. We evaluated expression of non-phosphorylated (active) and phosphorylated cofilin, LIMK1, LIMK2, and SSH1 by immunohistochemistry in 143 human CRC samples in relation to clinicopathologic parameters, response of metastatic disease to chemotherapy, and epithelial-mesenchymal transition (EMT) markers β-catenin, E-cadherin, and ZEB ...
A model from Chile, Maura Bellagamba, released a video on Instagram of herself being released in a giant slingshot, which she says caused her neck injuries while filming a Nissan car commercial.. Bellagamba wrote on Instagram that she was fired out of the slingshot without her permission, causing her whiplash ...
Remodeling of the actin cytoskeleton is involved in all motile and membrane trafficking processes, including cell migration, phagocytosis, macropinocytosis, endocytosis, exocytosis, and last, not least autophagy. This explains why actin and actin polymerization regulators constitute prime targets of bacterial pathogens, which have evolved to manipulate signaling pathways to actin cytoskeleton remodeling at various key nodes, foremost upstream Rho GTPase signaling, but also downstream actin polymerization and depolymerization factors. In my talk, I will address the molecular mechanisms of host cell responses to well-characterized Salmonella enterica virulence factors, including members of the Sop (Salmonella outer protein) family of proteins translocated into the host, and from there move on to less well understood mediators of bacterial pathogenesis and effects. ...
To confirm the morphological effects of 5-HT6Rs transfected in neuronal cells, we finally examined whether the activation of neuronal 5-HT6Rs produces effects on RhoA activities. To perform RhoA activity assay as shown in Fig. 2, we carried immunoblot analysis using cultured cortical neurons for sufficient amount of sample proteins. Here, we showed that 5-HT6R activation by ST1936 significantly increased RhoA-GTP level, which was significantly blocked by the pretreatment with SB258585 in cultured neurons (Figs. 4A and 4B). As downstream targets of Rho proteins, RhoA proteins can also affect actin polymerization by regulating cofilin via Rho-ROCK-LIMK-cofilin pathway (Lin et al., 2003). Therefore, we further executed whether the activation of 5-HT6Rs modulates cofilin activities as a downstream target of RhoA. As shown in Figs. 4C and 4D, we observed that treatment with ST1936 significantly increased phosphorylation of cofilin, which was also significantly blocked by SB258585 in cultured neurons. ...
To confirm the morphological effects of 5-HT6Rs transfected in neuronal cells, we finally examined whether the activation of neuronal 5-HT6Rs produces effects on RhoA activities. To perform RhoA activity assay as shown in Fig. 2, we carried immunoblot analysis using cultured cortical neurons for sufficient amount of sample proteins. Here, we showed that 5-HT6R activation by ST1936 significantly increased RhoA-GTP level, which was significantly blocked by the pretreatment with SB258585 in cultured neurons (Figs. 4A and 4B). As downstream targets of Rho proteins, RhoA proteins can also affect actin polymerization by regulating cofilin via Rho-ROCK-LIMK-cofilin pathway (Lin et al., 2003). Therefore, we further executed whether the activation of 5-HT6Rs modulates cofilin activities as a downstream target of RhoA. As shown in Figs. 4C and 4D, we observed that treatment with ST1936 significantly increased phosphorylation of cofilin, which was also significantly blocked by SB258585 in cultured neurons. ...
Abstract: A key aspect of cell and tissue morphogenesis is regulation of the actin cytoskeleton. The actin cytoskeleton is a fundamental cellular scaffold which...
Fads remind me of an iPad game called Slingshot Racing. What you do is, there are these tiny cars that run around a tiny oval racetrack, and you have to click on the screen (tech faux pas! tap on the screen) to tether the cars to tiny towers that slingshot them around the corners (ends? short sides?) of the oval. It sounds easy but in fact its devastatingly hard to tap and release at exactly the right times to start the tether and stop it without smashing your tiny car into a tiny wall. Thats kind of like catching and riding a fad. You have to discover that the potential for a fad exists, and not just any fad; one you can make money on, say by selling rocks or playing the saxophone in a particular way. You have to tether the fad to you at just the right moment, then hold onto it just up until the moment when its about to fade, at which point you release it before you start looking stupid. Few succeed at this game. Some tap too early and suffer ridicule, that is, until after theyre dead and ...
Ladies, and gentleman, I would like to present the winner of the Grey Cup, this year... Buffalo! Although the idea of a CFL commissioner making such a statement in the future seems far fetched, ...
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J:194042 Mahaffey JP, Grego-Bessa J, Liem KF Jr, Anderson KV, Cofilin and Vangl2 cooperate in the initiation of planar cell polarity in the mouse embryo. Development. 2013 Mar;140(6):1262-71 ...
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2019- 2020學年(上學期)????? (2019.10.14-2019.10.18)課題組名稱(聯系人)時? 間地? 點主講人題?? 目理論物理研究所 (李世淵)星期三15:30知新樓C913??交叉科學研究所 (劉向東)星期二下午離子束報告廳????????原子分子物理 (許國富)星期三下午知新樓C1011??????????凝聚態理論研究室 (崔彬)星期四14:30知新樓C1113季藝聞基于SSH模型對A-D-A類分子建模????電介質物理研究室 (趙明磊)星期五14:30知新樓C1113??...
... has been shown to interact with Cofilin 1. GRCh38: Ensembl release 89: ENSG00000120694 - Ensembl, May 2017 GRCm38: ... 869 (1-2): 49-55. doi:10.1016/S0006-8993(00)02346-5. PMID 10865058. Strausberg RL, Feingold EA, Grouse LH, et al. (2003). " ... 1444 (1): 138-42. doi:10.1016/s0167-4781(98)00254-1. PMID 9931472. "Entrez Gene: HSPH1 heat shock 105kDa/110kDa protein 1". ... The coding sequences of 80 new genes (KIAA0201-KIAA0280) deduced by analysis of cDNA clones from cell line KG-1 and brain". DNA ...
... signaling leads to phosphorylation of actin-interacting protein cofilin 1 at ser3; this may stabilize the actin ... "Reelin stabilizes the actin cytoskeleton of neuronal processes by inducing n-cofilin phosphorylation at serine3". The Journal ... 112 (1-2): 33-45. doi:10.1016/S0169-328X(03)00032-9. PMID 12670700. Hack I, Hellwig S, Junghans D, Brunne B, Bock HH, Zhao S, ... 1 (2): 72-73. doi:10.1046/j.1535-7597.2001.00017.x. PMC 320825. PMID 15309195. Zaki M, Shehab M, El-Aleem AA, Abdel-Salam G, ...
2004). "A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia". J. Cell Biol ... ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... 2007). "Coronin 1B coordinates Arp2/3 complex and cofilin activities at the leading edge". Cell. 128 (5): 915-29. doi:10.1016/j ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ...
In both yeast and mammals, CAPs appear to be involved in recycling G-actin monomers from ADF/cofilins for subsequent rounds of ... Moriyama K, Yahara I (April 2002). "Human CAP1 is a key factor in the recycling of cofilin and actin for rapid actin turnover ... A second unique function of CAP in regulating actin dynamics is its ability to specifically bind to pointed ends of cofilin- ... Shekhar S, Chung J, Kondev J, Gelles J, Goode BL (22 November 2019). "Synergy between Cyclase-associated protein and Cofilin ...
ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... 2005). "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin". EMBO J. 24 (3): ... 2007). "The slingshot family of phosphatases mediates Rac1 regulation of cofilin phosphorylation, laminin-332 organization, and ...
Vallenius, T; Luukko K; Mäkelä T P (Apr 2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha- ... Otey CA, Pavalko FM, Burridge K (1990). "An interaction between alpha-actinin and the beta 1 integrin subunit in vitro". J. ... Vallenius T, Luukko K, Mäkelä TP (2000). "CLP-36 PDZ-LIM protein associates with nonmuscle alpha-actinin-1 and alpha-actinin-4 ... An actin interface distinct from that of gelsolin domain 1 and from ADF/cofilin". Eur. J. Biochem. 268 (23): 6165-75. doi: ...
Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (Jun 1998). "Cofilin phosphorylation by LIM- ... Sumi T, Matsumoto K, Takai Y, Nakamura T (27 Dec 1999). "Cofilin phosphorylation and actin cytoskeletal dynamics regulated by ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (Aug 2001). "Cofilin phosphorylation and actin reorganization activities of ... Vardouli L, Moustakas A, Stournaras C (Mar 2005). "LIM-kinase 2 and cofilin phosphorylation mediate actin cytoskeleton ...
ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play ... The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin ... a family of phosphatases that dephosphorylate ADF/cofilin". Cell. 108 (2): 233-46. doi:10.1016/S0092-8674(01)00638-9. PMID ... "Interplay between components of a novel LIM kinase-slingshot phosphatase complex regulates cofilin". The EMBO Journal. 24 (3): ...
Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K (1998). "Cofilin phosphorylation by LIM-kinase 1 ... Birkenfeld J, Betz H, Roth D (January 2003). "Identification of cofilin and LIM-domain-containing protein kinase 1 as novel ... Nebl G, Meuer SC, Samstag Y (1996). "Dephosphorylation of serine 3 regulates nuclear translocation of cofilin". J. Biol. Chem. ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of ...
"Prion-like protein aggregates exploit the RHO GTPase to cofilin-1 signaling pathway to enter cells". The EMBO Journal. 37 (6). ... One of the inhibitors discovered in Bertolotti's lab, Sephin1, has passed through favourable Phase 1 clinical trials in 2019 ... Münch, Christian; O'Brien, John; Bertolotti, Anne (2011-03-01). "Prion-like propagation of mutant superoxide dismutase-1 ... "Exposure of hydrophobic surfaces initiates aggregation of diverse ALS-causing superoxide dismutase-1 mutants". Journal of ...
Spratley SJ, Bastea LI, Döppler H, Mizuno K, Storz P (September 2011). "Protein kinase D regulates cofilin activity through p21 ... 349 (1): 45-50. doi:10.1016/j.canlet.2014.03.024. PMID 24704155. Park JK, Kim S, Han YJ, Kim SH, Kang NS, Lee H, Park S (June ... 317 (1): 24-32. doi:10.1016/j.canlet.2011.11.007. PMID 22085492. Yeo D, Huynh N, Beutler JA, Christophi C, Shulkes A, Baldwin ... 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. Dan C, Nath N, Liberto M, Minden A (January 2002). "PAK5, a new ...
Birkenfeld J, Betz H, Roth D (January 2003). "Identification of cofilin and LIM-domain-containing protein kinase 1 as novel ... 9 (1): 143-54. doi:10.1089/jam.1996.9.143. PMID 10160204. Filoche M, Tai CF, Grotberg JB (July 2015). "Three-dimensional model ... 369 (Pt 1): 45-54. doi:10.1042/BJ20021152. PMC 1223062. PMID 12323073. Waterman MJ, Stavridi ES, Waterman JL, Halazonetis TD ( ... Koyama S, Williams LT, Kikuchi A (July 1995). "Characterization of the interaction of Raf-1 with ras p21 or 14-3-3 protein in ...
Enhanced docetaxel-mediated cytotoxicity in human prostate cancer cells through knockdown of cofilin-1 by carbon nanohorn ... 1 July 2016). "Design of Assembled Systems Based on Conjugated Polyphenylene Derivatives and Carbon Nanohorns". Chemistry - A ...
"Caspase-11 regulates cell migration by promoting Aip1-Cofilin-mediated actin depolymerization". Nature Cell Biology. 9 (3): 276 ... Production of IL-1β downstream of caspase-11 requires another canonical inflammasome, called the NLRP3 inflammasome, that ... The CARD domain of caspase-11 has been shown to associate with AIP-1 and cofilin to facilitate actin depolymerization. In ... This mechanism contrasts to that of the canonical inflammasome, in which a bacterial ligand activates caspase-1 through an ...
Toshima J, Toshima JY, Amano T, Yang N, Narumiya S, Mizuno K (2001). "Cofilin phosphorylation by protein kinase testicular ... Toshima J, Toshima JY, Takeuchi K, Mori R, Mizuno K (2001). "Cofilin phosphorylation and actin reorganization activities of ... Dual specificity testis-specific protein kinase 1 is an enzyme that in humans is encoded by the TESK1 gene. This gene product ... "Entrez Gene: TESK1 testis-specific kinase 1". Toshima JY, Toshima J, Watanabe T, Mizuno K (November 2001). "Binding of 14-3- ...
... cofilin 1 MeSH D12.776.220.525.212.750 - cofilin 2 MeSH D12.776.220.525.212.875 - destrin MeSH D12.776.220.525.246.500 - actin- ... neuregulin-1 See List of MeSH codes (D12.776.476). MeSH D12.776.503.280.249.500 - mannose-binding lectin MeSH D12.776.503.280. ... hiv-1 reverse transcriptase MeSH D12.776.964.970.880.325 - gene products, env MeSH D12.776.964.970.880.325.330 - hiv envelope ... neurofibromin 1 MeSH D12.776.402.150.500.500 - p120 gtpase activating protein MeSH D12.776.402.300.700.500 - ras-GRF1 MeSH ...
... cofilin 2 MeSH D05.750.078.730.212.875 - destrin MeSH D05.750.078.730.246 - actin-related protein 2-3 complex MeSH D05.750. ...
The product of this gene belongs to the actin-binding proteins ADF (Actin-Depolymerizing Factor)/cofilin family. This family of ... Furthermore, the binding of actin by destrin and cofilin is regulated negatively by phosphorylation. Destrin can also sever ... Destrin binds actin and is thought to connect it as gelsolin segment-1 does. ...
... like UNC-60A and cofilin like UNC-60B proteins of Caenorhabditis elegans". Biomolecular NMR Assignments. 9 (2): 261-265. doi: ... Shukla, Vaibhav Kumar; Kabra, Ashish; Yadav, Rahul; Ono, Shoichiro; Kumar, Dinesh; Arora, Ashish (1 October 2015). "NMR ... 1 February 2017). "Unraveling the stereochemical and dynamic aspects of the catalytic site of bacterial peptidyl-tRNA hydrolase ...
Cofilin 2 (muscle) also known as CFL2 is a protein which in humans is encoded by the CFL2 gene. Cofilin is a widely distributed ... Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN), all of which regulate ... "Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14". Ann. Hum ... 2006). "Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface". J. Mol. Biol. ...
... n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular ... "Entrez Gene: CFL1 cofilin 1 (non-muscle)". Chai X, Förster E, Zhao S, Bock HH, Frotscher M (January 2009). "Reelin stabilizes ... Lee K, Jung J, Kim M, Guidotti G (2001). "Interaction of the alpha subunit of Na,K-ATPase with cofilin". Biochem. J. 353 (Pt 2 ... Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 " ...
In accordance with their proposed rule, proteins like ADF, cofilin and LIMK1 are slingshot substrates. Phosphatases of ... Regenerating Liver (PRLs): Three PRL genes were described in mammals (PRL-1, PRL-2 and PRL-3). They share a high sequence ...
The latter is the enzyme that uses ATP to phosphorylate and inactivate the actin-depolymerizing factor cofilin. When cofilin is ... In the presence of Pyr1, LIMK1 is inhibited, which means that the phosphorylation of cofilin decreases, which results in the ... Pyr 1 is a cell permeable competitive inhibitor of Lim Kinase (especially LIMK1). ...
ADF/cofilin are the only substrates yet identified for the LIM kinases. LIM kinases directly phosphorylate and inactivate ... are actin-binding kinases that phosphorylate members of the ADF/cofilin family of actin binding and filament severing proteins ... LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal ... members of the cofilin family, resulting in stabilization of filamentous (F)-actin. Lim kinases are activated by signaling ...
Meanwhile, phosphorylation of PI3K by SRC activates RhoA which leads to activation of cofilin-P and disruption of filamentous ... When the WAVE-1 gene was disrupted in mice, it resulted in cognitive defects such as losses in learning and memory implicating ... 28 (1): 245-259. doi:10.1016/S0896-6273(00)00100-8. PMID 11086998. Tanaka, H; Shan, W; Phillips, GR; Arndt, K; Bozdagi, O; ... 51 (1): 43-56. doi:10.1016/j.neuron.2006.05.018. PMC 2587166. PMID 16815331. Ohkubo, T; Ozawa, M. (1999). "p120ctn Binds to the ...
... which then stimulates cofilin, which effectively reorganizes the actin cytoskeleton of the cell. In the case of neurons, ... The LINGO-1 structure has been shown to be highly stable both in its crystal form and in solution, thanks to its leucine-rich ... LINGO-1 is coded by the LINGO-1 gene, which is located on the human chromosome 15, more precisely on the locus 15q24-26, which ... LINGO-1 is expressed almost exclusively in the central nervous system (CNS). It can be found in the brain and in neurons and ...
2005). "Ribosomal protein S18 identified as a cofilin-binding protein by using phage display library". Mol. Cell. Biochem. 262 ... 239 (1): 144-9. doi:10.1111/j.1432-1033.1996.0144u.x. PMID 8706699. Andersen JS, Lyon CE, Fox AH, et al. (2002). "Directed ... 12 (1): 1-11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. S2CID 14132033. Mishra-Gorur K, Singer HA, Castellot JJ (2002 ... 1-2): 187-93. doi:10.1023/B:MCBI.0000038234.35936.1c. PMID 15532723. S2CID 30534195. Andersen JS, Lam YW, Leung AK, et al. ( ...
1999). "Aip1p interacts with cofilin to disassemble actin filaments". J. Cell Biol. 145 (6): 1251-64. doi:10.1083/jcb.145.6. ... 327 (1): 268-75. doi:10.1016/j.bbrc.2004.11.156. PMID 15629458. Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a ... 56 (1): 59-69. doi:10.1006/geno.1998.5672. PMID 10036186. "Entrez Gene: WDR1 WD repeat domain 1". Dawson SJ, White LA (1992). " ... 23 (1): 94-101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157. Fujibuchi T, Abe Y, Takeuchi T, et al. (2005). "AIP1/WDR1 ...
"Memo is a cofilin-interacting protein that influences PLCgamma1 and cofilin activities, and is essential for maintaining ... 78 (1-2): 46-54. doi:10.1006/geno.2001.6640. PMID 11707072. Qiu C, Lienhard S, Hynes NE, Badache A, Leahy DJ (February 2008). " ... "Entrez Gene: Mediator of cell motility 1". Meira M, Masson R, Stagljar I, Lienhard S, Maurer F, Boulay A, Hynes NE (March 2009 ... Mediator of cell motility 1 is a protein that in humans is encoded by the MEMO1 gene. GRCh38: Ensembl release 89: ...
核纤层蛋白 A/C(英语:Lamin A/C)是由人类基因LMNA 编码的蛋白质[1][2],属于核纤层蛋白家族。 ... 2000, 177 (1): 1-11. PMID 10960149. doi:10.1007/s002320001096.. *. Burke B, Mounkes LC, Stewart CL. The nuclear envelope in ... 2007, 53 (1): 46-52. PMID 17718387.. *. Genschel J, Schmidt HH. Mutations in the LMNA gene encoding lamin A/C. Hum. Mutat. ... Type 1/2
62 (1): 121-3. doi:10.1292/jvms.62.121. PMID 10676904.. *. Chakarova C, Wehnert MS, Uhl K, et al. (2001). "Genomic structure ... Shoeman RL, Hartig R, Hauses C, Traub P (2003). "Organization of focal adhesion plaques is disrupted by action of the HIV-1 ...
... was once thought to bind cofilin but is now believed to enhance the interactions between the V domains and actin monomers, as ... doi:10.1016/S0092-8674(00)80732-1. PMID 10219243.. *^ Thrasher AJ, Burns SO (March 2010). "WASP: a key immunological ... 120 (1): 2-9. doi:10.1046/j.1365-2249.2000.01193.x. PMC 1905602. PMID 10759756.. ...
67 (1): 37-46. doi:10.1086/302962. PMC 1287099. PMID 10841809.. *. De Jonghe P, Mersivanova I, Nelis E, Del Favero J, Martin JJ ... 45 (1): 30-2. doi:10.1159/000132421. PMID 3036423.. *. Frappier T, Regnouf F, Pradel LA (December 1987). "Binding of brain ... Pospelov VA, Pospelova TV, Julien JP (February 1994). "AP-1 and Krox-24 transcription factors activate the neurofilament light ... 10.1083/jcb.143.1.1. PMC 2132816. PMID 9763415.. *. Beaudet L, Charron G, Julien JP (May 1992). "Origin of the two mRNA species ...
Elastic filaments, 1 nm in diameter, are made of titin, a large springy protein. They run through the core of each thick ... Myofilaments are the filaments of myofibrils, constructed from proteins,[1] principally myosin or actin. Types of muscle are ...
Interaction of the alpha subunit of Na,K-ATPase with cofilin by K. Lee, J. Jung, M. Kim and G. Guidotti in The Biochemical ... 4.1/2/3/4/5/6 · 5.1/2/3/4/5/99 · 6.1-3(英语:Template:Ligases CO CS and CN)/4/5-6 ... 1]. 蛋白質交互作用對鈉鉀幫浦媒介的訊息傳遞是很重要的,如:鈉鉀幫浦與無接收子酪胺酸磷酸酶(Src,一種沒有受體得酪胺酸激酶)結合形成接收
26 (1): 103-5. doi:10.1038/79063. PMID 10973259.. *^ a b c d Seri M, Pecci A, Di Bari F, Cusano R, Savino M, Panza E, et al. ( ... 37 (1): 112-20. doi:10.1097/AUD.0000000000000198. PMID 26226608.. *^ a b Kunishima S, Matsushita T, Kojima T, Sako M, Kimura F ... 26 (1): 106-8. doi:10.1038/79069. PMID 10973260.. *^ Seri M, Cusano R, Gangarossa S, Caridi G, Bordo D, Lo Nigro C, et al. ( ... 142 (1): 221-30. PMC 1886840. PMID 8424456.. *. Lalwani AK, Linthicum FH, Wilcox ER, Moore JK, Walters FC, San Agustin TB, ...
Talin-1 is a protein that in humans is encoded by the TLN1 gene.[5][6] Talin-1 is ubiquitously expressed, and is localized to ... Human talin-1 is 270.0 kDa molecular weight and 2541 amino acids.[7] The N-terminal region of talin-1 is ~50 kDa in size and ... Talin-1 functions to mediate cell-cell adhesion via the linkage of integrins to the actin cytoskeleton and in the activation of ... In mammals talin-1 is ubiquitously expressed; talin-1 is found complexed to integrins and localized to intercalated discs of ...
11 (1): 45-53. doi:10.1016/S0955-0674(99)80006-4.. *^ Moughamian, Armen J.; Osborn, Gregory E.; Lazarus, Jacob E.; Maday, ... ISBN 0-471-19279-1.. *. Schroer TA (2004). "Dynactin". Annual Review of Cell and Developmental Biology. 20: 759-79. doi:10.1146 ... Cytoplasmic dynein helps to position the Golgi complex and other organelles in the cell.[1] It also helps transport cargo ... 1] Cytoplasmic dynein moves processively along the microtubule; that is, one or the other of its stalks is always attached to ...
Ankyrin-B, also known as Ankyrin-2, is a protein which in humans is encoded by the ANK2 gene.[1][2] Ankyrin-B is ubiquitously ... 1 (2): 93-9. doi:10.1161/circgenetics.108.792192. PMID 20031550.. *^ Alders, M; Christiaans, I; Pagon, RA; Adam, MP; Ardinger, ... ankyrin-1 has shown to be essential in normal function of erythrocytes;[10] however, ankyrin-B and ankyrin-3 play essential ... Mohler, PJ; Davis, JQ; Davis, LH; Hoffman, JA; Michaely, P; Bennett, V (26 March 2004). "Inositol 1,4,5-trisphosphate receptor ...
... , HB1, Hb-1, KRTHB1, MLN137, ghHkb1, hHAKB2-1, keratin 81. External IDs. OMIM: 602153 MGI: 1928858 HomoloGene: 55645 ... 2003). "Upregulation of the truncated basic hair keratin 1(hHb1-DeltaN) in carcinoma cells by Epstein-Barr virus (EBV)". Int. J ...
... s (/ˈmaɪəsɪn, -oʊ-/[1][2]) are a superfamily of motor proteins best known for their roles in muscle contraction and in a ... 51 (1): 1-15. doi:10.1002/cm.10014. PMID 11810692.. *^ von der Ecken J, Heissler SM, Pathan-Chhatbar S, Manstein DJ, Raunser S ... 46 (1): 61-7. doi:10.1002/ajmg.1320460110. PMID 7684189.. *^ "Archived copy". Archived from the original on 2009-07-07. ... 13 (1): 13-26. doi:10.1038/nrm3248. PMID 22146746. S2CID 11853457.. *^ Zimmermann D, Santos A, Kovar DR, Rock RS (August 2015 ...
CK-1. CK-2. CK-3. CK-4. CK-5. CK-6. CK-9. CK-10. CK-11. CK-12. CK-13. CK-14. CK-15. CK-16. CK-17 ... They are an important component of intermediate filaments, which help cells resist mechanical stress.[1] Expression of these ...
Cofilin-actin complex finally dissociate after cofilin phosphorylation by nuclear LIM kinase. ... The most notable of these proteins are gelsolin and cofilin. These proteins first achieve a cut by binding to an actin monomer ... The import of actin into the nucleus (probably in a complex with cofilin) is facilitated by the import protein importin 9.[96] ... They possess a number of accessory proteins including ADF/cofilin, which has a molecular weight of 16kDa and is coded for by a ...
... cofilin. ADP bound cofilin severs ADP-rich regions nearest the (−)-ends. Upon release, the free actin monomer slowly ... Actin depolymerizing proteins such as ADF/cofilin.. The actin filament network in non-muscle cells is highly dynamic. The actin ... cell signalling is believed to activate cofilin, the actin-filament depolymerizing protein which binds to ADP-rich actin ... ISBN 1-4160-2388-7.. *^ Galland R, Leduc P, Guérin C, Peyrade D, Blanchoin L, Théry M (2013). "Fabrication of three-dimensional ...
... was first described in 1976,[16] first purified in 1977,[17] the gene was cloned in 1989,[6] and the first knockout mouse was created in 1996.[18] The function of desmin has been deduced through studies in knockout mice. Desmin is one of the earliest protein markers for muscle tissue in embryogenesis as it is detected in the somites.[12] Although it is present early in the development of muscle cells, it is only expressed at low levels, and increases as the cell nears terminal differentiation. A similar protein, vimentin, is present in higher amounts during embryogenesis while desmin is present in higher amounts after differentiation. This suggests that there may be some interaction between the two in determining muscle cell differentiation. However desmin knockout mice develop normally and only experience defects later in life.[13] Since desmin is expressed at a low level during differentiation another protein may be able to compensate for desmin's function early in development but not ...
582 (1): 1-13. doi:10.1016/J.GENE.2016.01.006. PMC 5325693. PMID 26774798.. CS1 maint: Uses authors parameter (link). .mw- ... 1). Sequence homology and protein epitope allosteric similarity data suggest that TnT gene was originated by duplication of a ... 276 (5 Pt 1): C1162-70. doi:10.1152/ajpcell.1999.276.5.C1162. PMID 10329966.. ... 143 (1): 47-56. doi:10.1111/j.1432-1033.1984.tb08337.x. PMID 6468390.. ...
Cai L, Marshall TW, Uetrecht AC, Schafer DA, Bear JE (May 2007). "Coronin 1B coordinates Arp2/3 complex and cofilin activities ... Whereas coronin 1 was required for chemokine-mediated migration, it was dispensable for T cell antigen receptor functions in T ... 107 (1): 227-39. PMID 8175911. Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of ... N-terminus signature region is reduced to 5aa and appears in front of each WD-repeat core domain (e.g., CRN7, POD-1) Human ...
When the nascent endosome forms, the recruitment of cofilin by ClC-5 is a prerequisite for the localized dissolution of the ... Hryciw DH, Wang Y, Devuyst O, Pollock CA, Poronnik P, Guggino WB (October 2003). "Cofilin interacts with ClC-5 and regulates ... Hryciw DH, Wang Y, Devuyst O, Pollock CA, Poronnik P, Guggino WB (October 2003). "Cofilin interacts with ClC-5 and regulates ... It was demonstrated at the C-terminus of ClC-5 binds the actin-depolymerizing protein cofilin. ...
ADF/cofilins, which include ADF, cofilin, destrin, actophorin, coactosin, depactin and glia maturation factors (GMFs) beta and ... ADF/cofilins are small actin-binding proteins composed of a single ADF-H domain. They bind both actin-monomers and filaments ... ADF/cofilins bind ADP-actin with higher affinity than ATP-actin and inhibit the spontaneous nucleotide exchange on actin ... cofilin". J. Biol. Chem. 277 (45): 43089-95. doi:10.1074/jbc.M208225200. PMID 12207032. Liu LX, Xu H, Weller PF, Shi A, Debnath ...
Spine heads are enlarged due to the increased phosphorylation of cofilin, leading to a decrease in F-actin severing and ... 321 (1): 210-8. doi:10.1016/j.bbrc.2004.06.122. PMID 15358237. Rumbaugh G, Adams JP, Kim JH, Huganir RL (March 2006). "SynGAP ... 1005 (1-2): 44-50. doi:10.1016/j.brainres.2004.01.032. PMID 15044063. S2CID 33854259. Brandenberger R, Wei H, Zhang S, Lei S, ... 21 (12): 1-13. doi:10.1038/s41593-018-0268-0. ISSN 1546-1726. PMC 6309426. PMID 30455457. Vlaskamp DR, Shaw BJ, Burgess R, Mei ...
Madak-Erdogan, Zeynep; Ventrella, Rosa; Petry, Luke; Katzenellenbogen, Benita S (2014). "Novel Roles for ERK5 and Cofilin as ... 92 (1): 125-34. doi:10.1210/endo-92-1-125. PMID 4681918. "People , Carl R. Woese Institute for Genomic Biology". www.igb. ... 31 (1): 226-36. doi:10.1128/MCB.00821-10. PMC 3019850. PMID 20956553. Harrington, William R; Kim, Sung Hoon; Funk, Cory C; ... 105 (1): 33-40. doi:10.1210/endo-105-1-33. PMID 446414. Gong, Ping; Madak-Erdogan, Zeynep; Li, Jilong; Cheng, Jianlin; ...
LIM-kinase will in turn phosphorylate and inactivate cofilin, which will prevent the breakdown and recycling of actin filaments ... 28 (1-2): 65-76. doi:10.1007/s10555-008-9170-7. PMID 19160018. S2CID 33869424. Kimura, K.; Ito, M.; Amano, M.; Chihara, K.; ... 6 (1): 21-30. doi:10.1038/ncb1075. PMC 2605950. PMID 14647292. Maul, R. S. (February 3, 2003). "EPLIN regulates actin dynamics ... 6 (1): 275-302. doi:10.1146/annurev.bioeng.6.040803.140040. PMID 15255771. Chen, Christopher S.; Tan, John; Tien, Joe (August ...
... n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. Cofilin is a widely distributed intracellular ... "Entrez Gene: CFL1 cofilin 1 (non-muscle)". Chai X, Förster E, Zhao S, Bock HH, Frotscher M (January 2009). "Reelin stabilizes ... Lee K, Jung J, Kim M, Guidotti G (2001). "Interaction of the alpha subunit of Na,K-ATPase with cofilin". Biochem. J. 353 (Pt 2 ... Cofilin 1 has been shown to interact with HSPH1 and LIMK1. GRCh38: Ensembl release 89: ENSG00000172757 - Ensembl, May 2017 " ...
If you know of any papers that use this antibody, please contact us at antibodies [at] alzforum [dot] org for consideration in the References section.. ...
Homo sapiens cofilin pseudogene 1, mRNA (cDNA clone IMAGE:5168640). A. NR_028492.1. Homo sapiens cofilin 1 (non-muscle) ... Cofilin 1 (non-muscle) pseudogene 1 (CFL1P1). Human pseudo gene CFL1P1. Represented by 16 ESTs from 10 cDNA libraries. ...
"Determining the differences in actin binding by human ADF and cofilin.". Yeoh S., Pope B., Mannherz H.G., Weeds A.. J. Mol. ... "Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family.". Nakano K., Kanai ... "Cofilin phosphorylation and actin polymerization by NRK/NESK, a member of the germinal center kinase family.". Nakano K., Kanai ... "Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics.". Gohla A., Birkenfeld J. ...
The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and therefore potentially oncogenic missense mutations (click on Show Cancer Mutations). The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. ...
Proteintech Anti-Cofilin Polyclonal, Catalog # 10960-1-AP. Tested in Western Blot (WB), Immunofluorescence (IF), ... Cite Cofilin Polyclonal Antibody. The following product was used in this experiment: Cofilin Polyclonal Antibody from Thermo ... Gene Aliases: AA959946; CFL; CFL1; Cof; cofilin; HEL-S-15; n-cofilin ... Cofilin, non-muscle isoform; Cofilin-1; Cofilin1; epididymis secretory protein Li 15; p18 ...
D) Cofilin knockdown increased cell viability in As2O3-treated NB4-R1 cells. (E) Cofilin knockdown inhibited apoptosis in As2O3 ... there are two cofilin gene subtypes, cofilin-1 and cofilin-2, which encode different proteins. The former is expressed in a ... Cofilin-1 may play a key role in the mitochondrial apoptosis pathway and has the potential to be used as a drug target in APL ... Cofilin-1 may be involved in the resistance of NB4-R1 cells to retinoic acid, but hardly affect the pro-apoptotic potency of As ...
A pathway of neuregulin-induced activation of cofilin-phosphatase Slingshot and cofilin in lamellipodia. J Cell Biol 2004; 165 ... anti-phospho-cofilin (Ser3) and anti-cofilin rabbit polyclonal antibodies (Cell Signaling), anti-Flag M2 mouse monoclonal ... dephosphorylated cofilin is not necessarily active. We therefore analyzed whether depletion of SSH1L could restore cofilin ... thereby controlling the local dephosphorylation and activation of cofilin. In mammary breast cancer cells, initial cofilin ...
Both the cofilin-inhibitory peptides (Eibert et al., 2004) and cofilin S3D (Elam et al., 2015) bind F-actin and prevent ... Cofilin is activated by dephosphorylation of serine 3 (S3) (Mizuno, 2013). To assess the role of cofilin-mediated F-actin ... Rap1-GTP promotes cofilin dephosphorylation and stimulates cofilin-mediated actin severing. The resulting clearance of F-actin ... 3J,K). The ability of activated cofilin to bypass a defect in Rap1 activation argues that cofilin is the major downstream ...
"Cofilin-1 phosphorylation catalyzed by ERK1/2 alters cardiac actin dynamics in dilated cardiomyopathy caused by lamin A/C gene ... 4C). Expression of cofilin-2 in the heart was unchanged by overexpression of cofilin-1 or cofilin-1-T25A (Fig. 4C). Expression ... 4C). Expression of cofilin-2 in the heart was unchanged by overexpression of cofilin-1 or cofilin-1-T25A (Fig. 4C). Expression ... 4A). Expression of cofilin-2 in the heart was unchanged by over-expression of cofilin-1 (Fig. 4A). There was depolymerization ...
The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse ... The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse ... The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse ... The Rap1-cofilin-1 pathway coordinates actin reorganization and MTOC polarization at the B cell immune synapse ...
Liu L, Li J, Zhang L, Zhang F, Zhang R, Chen X, Brakebusch C, Wang Z, Liu X (2015) Cofilin phosphorylation is elevated after F‐ ... Mizuno K, Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E (1998) Cofilin phosphorylation by LIM‐kinase 1 ... induced activation of cofilin‐phosphatase Slingshot and cofilin in lamellipodia. J Cell Biol 165: 465-471. ... Upstream of cofilin1, signalling from the RHO GTPase and the ROCK1 and LIMK1 kinases controls cofilin1 activity to remodel ...
Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and ... It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004] ...
Dichotomous effects of the cofilin kinase LIMK1 on the early steps of HIV-1 infection of CD4 T cells. ... directly phosphorylates cofilin and regulates the actin cytoskeleton. Here, we investigated the role of LIMK1 in HIV-1 ... on resting CD4 T cells activates an actin-depolymerizing factor cofilin to promote the cortical actin dynamic critical for HIV ... HIV envelope-CXCR4 signaling activates cofilin to overcome cortical actin restriction in resting CD4 T cells. Cell. 134: 782-92 ...
In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2) ... Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such ... In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ... Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. This phosphorylation disrupts ...
Cofilin was used to normalize protein loading. D , E , Densitometric analysis of cofilin and LIMK1 phosphorylation in tissue ... Reelin-induced phosphorylation of n-cofilin involves Dab1, PI3K, and ApoER2. A , Reelin upregulates p-cofilin in E17.5 dab1 +/− ... Reelin increases n-cofilin phosphorylation in cultured hippocampal neurons. To visualize Reelin-induced n-cofilin ... The staining for serine3 phospho-cofilin in the marginal zone of reeler mutants suggests that cofilin may be phosphorylated by ...
Can bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is a major component of intranuclear and cytoplasmic actin rods ...
Epidermal Growth Factor Receptor-PI3K Signaling Controls Cofilin Activity To Facilitate Herpes Simplex Virus 1 Entry into ...
HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1. Proceedings ... HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1. / Quintela- ... title = "HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1", ... T1 - HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1 ...
immunogen = synthetic peptide: C-D I K D R S T L G E K, representing the C-terminus of the human protein according to NP_068733.1; NP_619579.1. ...
Cofilin 1 Pseudogene 8, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human ... CFL1P8 (Cofilin 1 Pseudogene 8) is a Pseudogene, and is affiliated with the lncRNA class. ... 1) Ensembl transcripts including schematic representations, and UCSC links where relevant :. * ENST00000436148 475 bps ...
... and given the described role of cofilin in actin filament polymerization and severing, we hypothesized that cofilin-1 activity ... and given the described role of cofilin in actin filament polymerization and severing, we hypothesized that cofilin-1 activity ... and given the described role of cofilin in actin filament polymerization and severing, we hypothesized that cofilin-1 activity ... and given the described role of cofilin in actin filament polymerization and severing, we hypothesized that cofilin-1 activity ...
Cofilin-1: A Modulator of Anxiety in Mice Martin Goodson, Marco B. Rust, Walter Witke, David Bannerman, Richard Mott, Chris P. ... Splicing Factors ASD-2 and SUP-12 Cooperatively Switch Alternative Pre-mRNA Processing Patterns of the ADF/Cofilin Gene in ... 1 segregation pattern called gene conversion. In this issue, Sun et al. use a visual, pollen tetrad-based assay to measure the ...
Mouse Monoclonal Anti-Cofilin Antibody (1A1) [DyLight 755]. Validated: WB, ELISA, ICC/IF, IHC, IHC-P. Tested Reactivity: Human ... Blogs on Cofilin. Check out the latest blog posts on Cofilin.. ACTB - an abundant cytoskeletal component with applications for ... Reviews for Cofilin Antibody (H00001072-M04IR) (0) There are no reviews for Cofilin Antibody (H00001072-M04IR). By submitting a ... Diseases for Cofilin Antibody (H00001072-M04IR). Discover more about diseases related to Cofilin Antibody (H00001072-M04IR). ...
... cofilin, profilin, fodrin, spectrin, tropomyosin, tropomodulin, myosin, actin buffer, actin polymerization buffer, actin ... Cofilin 1 protein: human recombinant sku :. CF01 Cofilin 1 protein: human recombinant ... Cofilin 1 protein: human recombinant (Cat. # CF01). van der Gucht, J., Paluch, E., Plastino, J. and Sykes, C. (2005). Stress ... Idrissi, F. Z., Wolf, B. L. and Geli, M. I. (2002). Cofilin, but not profilin, is required for myosin-I-induced actin ...
Cofilin 2 (muscle) also known as CFL2 is a protein which in humans is encoded by the CFL2 gene. Cofilin is a widely distributed ... Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN), all of which regulate ... "Mapping of human non-muscle type cofilin (CFL1) to chromosome 11q13 and muscle-type cofilin (CFL2) to chromosome 14". Ann. Hum ... 2006). "Cofilin cross-bridges adjacent actin protomers and replaces part of the longitudinal F-actin interface". J. Mol. Biol. ...
Barone, Eugenio and Mosser, Sebastien and Frearing, Patrick C (2014) Cofilin-1 activity is modulated by age, Alzheimers ... Cofilin-1 activity is modulated by age, Alzheimers disease pathology and gamma secretase ...
2000 Antifreeze Proteins by Bill Nye for National Science Foundation.oga 1 min 3 s; 508 KB. ... 2ph1 pdb gallery nucleotide binding-protein-2 (MinD homolog).svg 1 101 × 745; 302 KB. ... 2L6X Active Site Visualization E.Vitureira.png 1 054 × 770; 275 KB. ... У гэтай катэгорыі ёсць наступныя 200 файлаў з агульнага ліку 1 125. ...
Non-overlapping activities of ADF and cofilin-1 during the migration of metastatic breast tumor cells. . Biblioteca virtual ... Focal adhesion area and cell adhesion in cofilin KD cells were returned to control levels by expressing exogenous cofilin but ... Non-overlapping activities of ADF and cofilin-1 during the migration of metastatic breast tumor cells - Lubna H Tahtamouni - ... whereas in cofilin KD cells, expression of cofilin efficiently rescued control migration rates.. ConclusionAlthough ADF and ...
... amino acid homology with cofilin-2, a muscle type of cofilin. This high homology suggests that cofilin-2 would be a real autoAg ... The anti-cofilin-2 antibodies may easily cross-react with cofilin-1. It is reported that cofilins inhibit selleck screening ... In BD patients, antibodies to cofilin-2 may react with cofilin-1 through the amino acid homology. However, in contrast to PM/DM ... activation in mucocutaneous lesions, Doxorubicin mouse including the aphtha (31). In the WB using cofilin-MBP, the anti-cofilin ...
  • n-cofilin), also known as CFL1, is a human gene, part of the ADF/cofilin family. (wikipedia.org)
  • CFL1 (AAH11005, 1 a.a. - 166 a.a.) full-length recombinant protein with GST tag. (novusbio.com)
  • Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN), all of which regulate actin-filament dynamics. (wikipedia.org)
  • To investigate the necessity of cofilin-1 in the glomerulus, podocyte-specific Cfl1 null mice were generated. (elsevier.com)
  • The protein spots cofilin-1 (CFL1) and glycealdehyde-3-phosphate dehydrogenase (GAPD) from cMSCs had expression levels similar to that of ACs whereas the others (ie. (medsci.org)
  • Accordingly, PKD controls migration of breast cancer cells via the SSH1L-cofilin signaling pathway. (aacrjournals.org)
  • β -Actin is a downstream effector of the PI3K/AKT signaling pathway in myeloma cells," Molecular and Cellular Biochemistry , vol. 348, no. 1-2, pp. 129-139, 2011. (hindawi.com)
  • A synthetic peptide corresponding to amino acid residues at the N-terminus of human Cofilin 1. (ecmbio.com)
  • This sequence has 100% homology to similar regions in rat and mouse Cofilin 1, and has 3 amino acid differences from human Cofilin 2. (ecmbio.com)
  • Synthetic peptide within Human Cofilin aa 1-100. (abcam.com)
  • Synthetic peptide: C-QVNGLDDIKDRSTLGEK conjugated to KLH, corresponding to amino acids 136-152 (plus N terminal cysteine) of Human Cofilin 2. (genetex.com)
  • Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Cofilin. (abcam.com)
  • GenScript Rabbit Anti-Cofilin Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 100-150 of human cofilin protein (Swiss Prot: P23528). (genscript.com)
  • Here we report atomic-level characterization by magic angle spinning (MAS) NMR of the muscle isoform of human cofilin 2 (CFL2) bound to F-actin. (antikoerper-online.de)
  • Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Cofilin 2. (genetex.com)
  • The following product was used in this experiment: Cofilin Polyclonal Antibody from Thermo Fisher Scientific, catalog # 10960-1-AP. (thermofisher.com)
  • Antibodies used were as follows: anti-PKD2 rabbit polyclonal antibody (Calbiochem), anti-PKD C-20 (Santa Cruz Biotechnology), anti-phospho-cofilin (Ser3) and anti-cofilin rabbit polyclonal antibodies (Cell Signaling), anti-Flag M2 mouse monoclonal antibody (Sigma-Aldrich, Germany), anti-tubulin mouse monoclonal antibody (Neomarkers), anti-SSH1L rabbit polyconal antibody (Abcam), and anti-glutathione S -transferase (GST) goat polyclonal antibody (GE Healthcare). (aacrjournals.org)
  • There are no publications for Cofilin Antibody (H00001072-M04IR). (novusbio.com)
  • The peptide is specifically recognized by anti-Cofilin 1 (N-terminus) antibody (CP1131) in ELISA, and is recommended for use in blocking CP1131 reactivity in Western blot and immunocytochemistry. (ecmbio.com)
  • GenScript Rabbit Anti-Cofilin Polyclonal Antibody detects endogenous levels of human and mouse cofilin protein. (genscript.com)
  • Membranes were probed with either Prdx-1 antibody (anti-Prdx-1 Ab) or Prdx-4 Ab (anti-Prdx-4 Ab) (left) or immunogen peptide-preadsorbed Prdx-1 and Prdx-4 Abs (right). (asm.org)
  • The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cofilin (D3F9) XP ® Rabbit mAb #5175. (cellsignal.com)
  • Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Cofilin 2(GTX100213) antibody at 1:100 dilution. (genetex.com)
  • Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Cofilin 2(GTX100213) antibody at 1:200 dilution. (genetex.com)
  • Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody (GTX100213) diluted at 1:1000. (genetex.com)
  • There are currently no references for Cofilin 2 antibody (GTX100213) . (genetex.com)
  • We show that active phosphorylated ERK1/2 directly binds to and catalyzes the phosphorylation of the actin depolymerizing factor cofilin-1 on Thr25. (deepdyve.com)
  • Actin-depolymerization factor/cofilin activity is also regulated by other actin-binding proteins, activity-dependent subcellular distribution and protein translation. (frontiersin.org)
  • We showed that aberrantly increased activity of the Rho GTPase Rac1 inhibited the actin-depolymerizing factor cofilin, a major determinant of dendritic spine structure, and caused disease-associated spine abnormalities in the somatosensory cortex of FXS model mice. (sciencemag.org)
  • Actin-depolymerizing factor cofilin-1 is necessary in maintaining mature podocyte architecture," Journal of Biological Chemistry , vol. 285, no. 29, pp. 22676-22688, 2010. (hindawi.com)
  • The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. (wikipedia.org)
  • Phosphorylation of actin-depolymerizing factor/cofilin by LIM-kinase mediates amyloid beta-induced degeneration: a potential mechanism of neuronal dystrophy in Alzheimer's disease. (springer.com)
  • Xenopus actin depolymerizing factor/cofilin (XAC) is responsible for the turnover of actin filaments in Listeria monocytogenes tails. (harvard.edu)
  • PKD-mediated phosphorylation of serines 937 and 978 regulates SSH1L subcellular localization by binding of 14-3-3 proteins and thus impacts the control of local cofilin activation and actin remodeling during cell migration. (aacrjournals.org)
  • These cytoskeletal changes are regulated by actin-binding proteins such as cofilin, an essential regulator of actin dynamics ( Bamburg, 1999 ). (jneurosci.org)
  • Members of the ADF/cofilin (AC) family are actin-severing proteins that regulate actin remodeling during cellular events such as cell migration, cytokinesis, phagocytosis, endocytosis, axon development, and immune cell activation. (ecmbio.com)
  • Actin-depolymerization factor (ADF)/cofilin, a family of actin-binding proteins, are critical for the regulation of actin reorganization in response to various signals. (frontiersin.org)
  • BackgroundADF-cofilin proteins are key modulators of actin dynamics in metastasis and invasion of cancer cells. (duhnnae.com)
  • The decrease in Rac1/cdc42 activity was paralleled by decreases in the phosphorylation of the downstream effectors of these proteins, such as LIMK-1 and cofilin. (pubmedcentralcanada.ca)
  • The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al. (wikipedia.org)
  • Early neuritic cytoskeletal defects determined by the presence of phosphorylated tau (AT8-positive) and actin-cofilin rods along with decreased levels of kinesin-1 and dynein motor proteins could be responsible for this extensive vesicle accumulation within dystrophic neurites. (springer.com)
  • A member of the cofilin family of proteins that is expressed in MUSCLE CELLS. (harvard.edu)
  • In this study, we show that PKD directly phosphorylates and regulates slingshot (SSH) 1 like (SSH1L), a phosphatase that activates the actin depolymerizing protein cofilin by dephosphorylation. (aacrjournals.org)
  • Accordingly, dephosphorylation by the SSH as well as chronophin phosphatases results in reactivation of the actin binding activity of cofilin ( 6 ). (aacrjournals.org)
  • We now provide evidence that PKD1 and PKD2 directly phosphorylate SSH1L at these residues, thereby controlling SSH1L localization and thus cofilin dephosphorylation and activation at membrane protrusions. (aacrjournals.org)
  • Nephrin activation induced cofilin dephosphorylation via intermediaries that include phosphatidylinositol 3-kinase, SSH1, 14-3-3, and LIMK in a cell culture model. (elsevier.com)
  • In a similar fashion, cofilin-1 dephosphorylation was observed in a rat model of podocyte injury at a time when foot process spreading is initially observed. (elsevier.com)
  • Journal Article] Insulin receptor substrate-4 binds to Slingshot-1 phosphatase and promotes cofilin dephosphorylation. (nii.ac.jp)
  • Journal Article] Signaling mechanisms and functional roles of cofilin phosphorylation and dephosphorylation. (nii.ac.jp)
  • The protein kinase D (PKD) family of serine/threonine kinases consists of three isoforms: PKD1, PKD2, and PKD3 ( 1 ). (aacrjournals.org)
  • Cofilin phosphorylation by LIM kinases and testicular protein kinase at serine 3 turns off the actin-binding activity of cofilin and thus leads to inactivation. (aacrjournals.org)
  • Upstream of cofilin‐1, signalling from the RHO GTP ase and the ROCK 1 and LIMK 1 kinases controls cofilin‐1 activity to remodel actin and modulate aggregate entry. (embopress.org)
  • We provide evidence for ApoER2, Dab1, Src family kinases (SFKs), and PI3K to be involved in n-cofilin serine3 phosphorylation. (jneurosci.org)
  • Activation of cofilin kinases, LIMK1 or LIMK2, leads to phosphorylation of cofilin at serine 3. (ecmbio.com)
  • Regulation of ADF/cofilin involves various signaling pathways converging on LIM domain kinases and slingshot phosphatases, which phosphorylate/inactivate and dephosphorylate/activate ADF/cofilin, respectively. (frontiersin.org)
  • Rac1 activated the kinases PAK and LIMK1, which inactivated cofilin, thus preventing actin depolymerization dynamics. (sciencemag.org)
  • The anti-cofilin-2 antibodies may easily cross-react with cofilin-1. (hsp90inhibitors.com)
  • Thus, cofilin-2 is deeply involved in the function of myocytes and the probable generation of anti-cofilin-2 autoAbs may damage myocytes in patients with PM/DM. (hsp90inhibitors.com)
  • In the present study, on the clinical symptoms and laboratory parameters, no significant difference was found between anti-cofilin-1-positive and -negative patients in each of the disease categories tested. (hsp90inhibitors.com)
  • This is possibly due to the relatively small numbers of anti-cofilin-1 autoAb-positive patients (i.e. only 2-8 out of 30 or more patients were positive for the autoAbs). (hsp90inhibitors.com)
  • Phosphorylation at serine3 renders n-cofilin unable to depolymerize F-actin, thereby stabilizing the cytoskeleton. (jneurosci.org)
  • The LIM domain kinase 1 (LIMK1) directly phosphorylates cofilin and regulates the actin cytoskeleton. (biomedcentral.com)
  • Adhesion by α5β1 instead leads to the phosphorylation/inactivation of cofilin, and these cells fail to polarize their cytoskeleton but extend thin protrusions containing highly dynamic cell-matrix adhesions in multiple directions. (rupress.org)
  • Rearrangements of the cytoskeleton and cell contacts induce process formation during differentiation of conditionally immortalized mouse podocyte cell lines," Experimental Cell Research , vol. 236, no. 1, pp. 248-258, 1997. (hindawi.com)
  • Cofilin-1 is a major mediator of cytoskeleton dynamics as it regulates the remodeling of actin filaments (Carlier et al. (springer.com)
  • We have not come up with an action mechanism, but we confirmed that the inhibition of the phosphorylation pathway of cofilin 1 prevents exposure to beta amyloid peptides from causing the deactivation of the protein, and the consequent effect on the cytoskeleton of the dendritic spines," said Martínez. (innovations-report.com)
  • 2013. Mammalian CAP1 (adenylyl Cyclase-Associated Protein 1) regulates cofilin function, the actin cytoskeleton and cell adhesion. (cytoskeleton.com)
  • 2014. Gamma interferon-induced guanylate binding protein 1 is a novel actin cytoskeleton remodeling factor. (cytoskeleton.com)
  • Cofilin 1 has been shown to interact with HSPH1 and LIMK1. (wikipedia.org)
  • Signaling through RHO GTPase, ROCK1 and LIMK1 to cofilin‐1 remodels actin to facilitate aggregate entry. (embopress.org)
  • Here, we investigated the role of LIMK1 in HIV-1 infection of resting CD4 T cells and found that HIV-1 infection triggered a rapid, transient activation of LIMK1. (biomedcentral.com)
  • Cofilin, a LIMK1 substrate, is essential for the regulation of actin polymerization and depolymerization during cell migration. (rupress.org)
  • We resolve this paradox by showing that the effects of LIMK1 expression on migration, intravasation, and metastasis of cancer cells can be most simply explained by its regulation of the output of the cofilin pathway. (rupress.org)
  • LIMK1-mediated decreases or increases in the activity of the cofilin pathway are shown to cause proportional decreases or increases in motility, intravasation, and metastasis of tumor cells. (rupress.org)
  • Cofilin-1 phosphorylation catalyzed by ERK1/2 alters cardiac actin dynamics in dilated. (deepdyve.com)
  • These results demonstrate a novel role for cofilin-1 on actin dynamics in cardiac muscle and provide a rationale on how increased ERK1/2 signaling leads to LMNA cardiomyopathy. (deepdyve.com)
  • Among the regulators of actin, cofilins, which are actin-depolymerizing factors, play an essential role in the dynamics of filaments. (deepdyve.com)
  • In the spinal cord of symptomatic SOD 1 G93A transgenic mice, cofilin‐1 phosphorylation is increased and actin dynamics altered. (embopress.org)
  • Because podocyte intercellular junction receptor Nephrin plays a role in regulating actin dynamics, and given the described role of cofilin in actin filament polymerization and severing, we hypothesized that cofilin-1 activity is regulated by Nephrin and is necessary in normal podocyte actin dynamics. (elsevier.com)
  • Our results define a mechanism for the regulation of cofilin and hence of actin dynamics in vivo. (nih.gov)
  • ConclusionAlthough ADF and cofilin have many redundant functions, each of these isoforms has functional differences that affect F-actin structures, cell adhesion and lamellipodial dynamics, all of which are important determinants of cell migration. (duhnnae.com)
  • It was also reported that cofilin plays an essential role in the control of phagocyte function through regulation of actin filament dynamics (30). (hsp90inhibitors.com)
  • Over-expression of cofilin-1 may disrupt the balance of actin dynamics and lead to obstacle of cell motility. (clinicsinoncology.com)
  • The three mouse actin-depolymerizing factor/cofilins evolved to fulfill cell-type-specific requirements for actin dynamics," Molecular Biology of the Cell , vol. 13, no. 1, pp. 183-194, 2002. (hindawi.com)
  • The actin depolymerizing factor (ADF)/cofilin protein family is essential for actin dynamics, cell division, chemotaxis and tumor metastasis. (mdpi.com)
  • In response to environmental stimulation, CFL-1 enters the nucleus to regulate the actin dynamics. (mdpi.com)
  • Actin dynamics and cofilin-actin rods in Alzheimer disease. (springer.com)
  • Downregulated in renal cell carcinoma 1 (DRR1) protein was characterized as the link between stress, actin dynamics, neuronal function, and cognition. (mdpi.com)
  • Actin-depolymerizing factor (ADF)/cofilins, which sever and depolymerize aged actin filaments, are also central regulators of cytoskeletal dynamics in many cell types ( Bamburg, 1999 ). (rupress.org)
  • Cofilin is a major regulator of actin dynamics involved in the regulation of cell spreading and migration through its actin depolymerizing and severing activities. (pubmedcentralcanada.ca)
  • COTL1 regulates lamellipodia dynamics in part by protecting F-actin from cofilin-mediated disassembly. (genscript.com)
  • Cofilin 2 is the muscle isoform. (genetex.com)
  • Cofilin-1 (CFL-1) is a primary non-muscle isoform of the ADF/cofilin protein family accelerating the actin filamental turnover in vitro and in vivo . (mdpi.com)
  • We previously demonstrated that extracellular signal-regulated kinase (ERK) 1/2 is hyper-activated in the heart in LMNA cardiomyopathy (7). (deepdyve.com)
  • Steps requiring additional studies, such as involvement of CCR5 in cofilin activation, regulation of a switch between activation of cofilin kinase and phosphatase, the role of F-actin in HIV reverse transcription and nuclear translocation are marked by question marks. (nih.gov)
  • This Nephrin-induced cofilin activation required a direct interaction between Nephrin and the p85 subunit of phosphatidylinositol 3-kinase. (elsevier.com)
  • LIM-kinase 1, also known as KIZ, is a protein kinase with two amino-terminal LIM motifs that induces stabilization of F-actin structures in transfected cells. (nih.gov)
  • Phosphorylation experiments in vivo and in vitro provide evidence that cofilin is a physiological substrate of LIM-kinase 1. (nih.gov)
  • Phosphorylation by LIM-kinase 1 inactivates cofilin, leading to accumulation of actin filaments. (nih.gov)
  • Constitutively active Rac augmented cofilin phosphorylation and LIM-kinase 1 autophosphorylation whereas phorbol ester inhibited these processes. (nih.gov)
  • Email: [email protected] Abstract Hyper-activation of extracellular signal-regulated kinase (ERK) 1/2 contributes to heart dysfunction in cardiomyopathy caused by mutations in the lamin A/C gene (LMNA cardiomyopathy). (readkong.com)
  • We previ- ously demonstrated that extracellular signal-regulated kinase (ERK) 1/2 is hyper-activated in the heart in LMNA cardiomyopa- thy (7). (readkong.com)
  • One of the pathways of cofilin 1 phosphorylation is a kinase known as ROCK, a kind of enzyme that modifies other molecules by means of phosphorylation, sometimes activating them and other times deactivating them. (innovations-report.com)
  • Adhesion to fibronectin by αvβ3 supports extensive actin cytoskeletal reorganization through the actin-severing protein cofilin, resulting in a single broad lamellipod with static cell-matrix adhesions at the leading edge. (rupress.org)
  • Oxidant-induced apoptosis is mediated by oxidation of the actin-regulatory protein cofilin. (springer.com)
  • Enhancement of radiosensitivity in H1299 cancer cells by actin-associated protein cofilin. (springer.com)
  • Cofilin 2 (muscle) also known as CFL2 is a protein which in humans is encoded by the CFL2 gene. (wikipedia.org)
  • Auf www.antikoerper-online.de finden Sie aktuell 94 Cofilin 2 (Muscle) (CFL2) Antikörper von 23 unterschiedlichen Herstellern. (antikoerper-online.de)
  • Normal myofibrillar development followed by progressive sarcomeric disruption with actin accumulations in a mouse Cfl2 knockout demonstrates requirement of cofilin-2 for muscle maintenance. (harvard.edu)
  • Regulation of cofilin activity can occur through serine phosphorylation. (ecmbio.com)
  • Thus, Ser-3 phosphorylation is a major site for the regulation of cofilin activity. (ecmbio.com)
  • Mechanistically, HUNK overexpression sustained the constitutive phosphorylation and inactivation of cofilin-1 (CFL-1), thereby blocking the incorporation of new actin monomers into actin filaments. (elsevier.com)
  • Our results support the idea that the damage caused by the beta amyloid peptides on the level of the dendritic spines during the early stages of the disease may be prevented by modulating ROCK and cofilin 1, and that, therefore, research needs to be conducted into medications that specifically stop that phosphorylation of cof1 in neurons, so that future medical treatments to combat Alzheimer's disease can be produced," concluded Dr Martínez. (innovations-report.com)
  • C) Prdx-1 Ab specificity. (asm.org)
  • Moreover, cofilin‑1 knockdown by its specific short hairpin RNA significantly suppressed As2O3‑induced NB4‑R1 cell apoptosis and inhibited the release of mitochondrial cytochrome C. Whereas, overexpression of cofilin‑1 using a plasmid vector carrying cofilin‑1 increased the release of cytochrome C into the cytoplasm from the mitochondria in As2O3‑treated NB4‑R1 cells. (spandidos-publications.com)
  • Small interference RNA siRNA technology was used to knockdown KD the expression of ADF and cofilin-1 independently. (duhnnae.com)
  • Interestingly, knockdown of cofilin, a known substrate of PP1 containing a nuclear localization signal, also prevented nuclear accumulation of NF-B. Expression of a constitutively active nonphosphorylatable S3A-cofilin in D95N-PP1 cells restored nuclear translocation of NF-B and IL-10 expression. (mazenz.com)
  • siRNA-mediated knockdown of Prdx-1 and Prdx-4 in RSV-treated A549 cells. (asm.org)
  • Enhanced docetaxel-mediated cytotoxicity in human prostate cancer cells through knockdown of cofilin-1 by carbon nanohorn delivered siRNA. (semanticscholar.org)
  • Here, we show that Reelin signaling leads to serine3 phosphorylation of n-cofilin, an actin-depolymerizing protein that promotes the disassembly of F-actin. (jneurosci.org)
  • The subsequent release of inorganic phosphate (P i ) destabilizes the filament and promotes actin filament disassembly ( 1 , 2 ). (pnas.org)
  • Actin filament nucleation through the Arp2/3 complex subsequently promotes spine head expansion, and ADF/cofilin-induced actin filament disassembly is required to maintain proper spine length and morphology. (rupress.org)
  • We previously found that over-expression of cofilin-1, an actin binding protein belongs to the actin depolymerizing factor (ADF)/cofilin family leads to morphological change and inhibition of invasion of human non-small cells lung cancer (NSCLC). (clinicsinoncology.com)
  • Inhibition of the Rac1 effector PAK1 with a small-molecule inhibitor rescued cofilin signaling in FXS mice, indicating a causal relationship between PAK1 and cofilin signaling. (sciencemag.org)
  • Lastly, inhibition of cell spreading by v-Src was rescued partially by co-expression of cofilin, and to a greater extent by the Y68F mutant which is not subjected to v-Src induced degradation through phosphorylation, suggesting that v-Src mediated changes in cell spreading is, at least in part, through inhibiting the function of cofilin via phosphorylating it at Y68. (pubmedcentralcanada.ca)
  • HUNK suppresses metastasis of basal type breast cancers by disrupting the interaction between PP2A and cofilin-1," Proceedings of the National Academy of Sciences of the United States of America , vol. 107, no. 6, pp. 2622-2627, 2010. (hindawi.com)
  • Wang W., Eddy R., Condeelis J. The cofilin pathway in breast cancer invasion and metastasis. (springer.com)
  • This family includes cofilin-1 (n-cofilin, non-muscle), cofilin-2 (muscle cofilin) and actin-depolymerization factor (ADF, destrin) and is well conserved among eukaryotes ( Maciver and Hussey, 2002 ). (frontiersin.org)
  • 1. Lei JZ, Nie Q, Chen DB (2018) A single-cell epigenetic model for parental psychological stress-induced transgenerational reprogramming in offspring. (uci.edu)
  • In this study, we provide evidence that the cofilin phosphatase slingshot 1 like (SSH1L), an important regulator of the complex actin remodeling machinery, is a novel in vivo PKD substrate. (aacrjournals.org)
  • Together these findings reveal novel functions for PP1α and its substrate cofilin in T cells namely the regulation of the nuclear translocation of NF-B and promotion of IL-10 production. (mazenz.com)
  • Journal Article] Activation of cytosolic Slingshot-1 phosphatase by gelsolin-generated soluble actin filaments. (nii.ac.jp)
  • The data obtained demonstrated that mDia1-FH2 induced actin repolymerization only from the actin/cofilin-1 complex, but not when complexed to actin depolymerizing factor, gelsolin segment 1, vitamin D binding protein, or deoxyribonuclease I. (elsevier.com)
  • Introduction Mutations in the lamin A/C gene (LMNA) cause an autosomal dominant inherited form of dilated cardiomyopathy (hereafter referred to as LMNA cardiomyopathy), often with concurrent muscular dystrophy (1,2). (deepdyve.com)
  • The molecular events that accompany meiotic recombination can also cause a nonreciprocal exchange of information between parental chromosomes that results in a non-Mendelian 3:1 segregation pattern called gene conversion. (plos.org)
  • To monitor the effects of cofilin-1 and let-7 on lung cancer migration in vivo, we established a multiple reporter genes transduced lung cancer cell line that can be detected using the reporter gene imaging. (clinicsinoncology.com)
  • The primary cause of FXS is an unstable trinucleotide CGG repeat expansion of the fragile X mental retardation 1 ( FMR1 ) gene, leading to its hypermethylation and transcriptional silencing ( 4 , 5 ). (sciencemag.org)
  • Tatsuka & Mitsui in "Elongation factor-1 alpha gene determines susceptibility to transformation" (Nature, 1992, vol. 359, pp. 333-336). (freepatentsonline.com)
  • The 3′-end of the human β-actin gene enhances activity of the β-actin expression vector system: construction of improved vectors," Journal of Biochemical and Biophysical Methods, 1997, vol. 36, No. 1, pp. 63-72. (freepatentsonline.com)
  • For the SSH-1 protocol, see Secure Shell#Version 1.x Protein phosphatase Slingshot homolog 1 is an enzyme that in humans is encoded by the SSH1 gene. (wikipedia.org)
  • Cofilin nucleates actin polymerization by severing actin filaments to generate free barbed ends and also increases the rate of actin depolymerization, thus maintaining a pool of actin monomers ( 4 , 5 ). (aacrjournals.org)
  • In this review we will focus on the role of actin-depolymerization factor (ADF)/cofilin in the regulation of LTP, LTD, dendritic spines and their dysfunction in Alzheimer's disease (AD). (frontiersin.org)
  • Cofilin 2 phosphorylation and genetic overexpression plays a role in the pathogenesis of idiopathic dilated cardiomyopthay. (antikoerper-online.de)
  • The cofilin phosphatase SSH1L is an ubiquitously expressed phosphatase implicated in diverse cellular processes such as regulation of membrane protrusions and mitosis ( 7 ). (aacrjournals.org)
  • HUNK reconstitution in basal breast cancer cell lines prevented protein phosphatase 2-A (PP2A), a phosphatase putatively acting on CFL-1, from binding to CFL-1. (elsevier.com)
  • HIV-induced signaling from CXCR4 activates phosphatase which dephosphorylates and activates cofilin. (nih.gov)
  • Here, we analyzed the involvement of protein phosphatase 1α (PP1α) in cytokine synthesis following costimulation of primary human T cells. (mazenz.com)
  • The results demonstrated that in retinoic acid‑resistant NB4‑R1 cells, the protein expression of cofilin‑1 was markedly increased compared with that in the drug‑sensitive NB4 cells. (spandidos-publications.com)
  • In vivo expression of cofilin-1, phosphorylated on Thr25 by endogenous ERK1/2 signaling, leads to alterations in left ventricular function and cardiac actin. (deepdyve.com)
  • Return to control rates of cell migration in ADF KD cells was achieved by expression of exogenous ADF but not cofilin, whereas in cofilin KD cells, expression of cofilin efficiently rescued control migration rates. (duhnnae.com)
  • Over-Expression of Cofilin-1 Suppressed Mobility of Lung Cancer Cells is Associated with DownRegulation of SNAIL-1 and Induction of Let-7. (clinicsinoncology.com)
  • Over-expression of cofilin-1 in human H1299 lung cancer cells also suppressed SNAIL-1 transcription factors, but E-cadherin and N-cadherin were not significantly affected. (clinicsinoncology.com)
  • Importantly, over-expression of cofilin-1 induced let-7 could be suppressed by enforced expression of SNAIL-1, suggesting that EMT related transcription factors can be suppressed by over-expressed cofilin-1 to induce let-7 expression. (clinicsinoncology.com)
  • However, over-expression of cofilin-1 may not suppress EMT. (clinicsinoncology.com)
  • Compared to normal lung cancer cells, over-expression of cofilin-1 suppressed the lung cancer migration, but simultaneously transfection of LNA recovered their migration ability to lungs in small animals. (clinicsinoncology.com)
  • Taken together, over-expression of cofilin-1 can suppress the invasion and migration of lung cancer cells through up-regulation of let-7 in vitro and in vivo . (clinicsinoncology.com)
  • Regulated expression of cofilin and the consequent regulation of p27 kip1 are essential for G1 phase progression," Cell Cycle , vol. 8, no. 15, pp. 2365-2374, 2009. (hindawi.com)
  • Viral delivery of a constitutively active cofilin mutant (cofilinS3A) into the somatosensory cortex of Fmr1 -deficient mice rescued the immature dendritic spine phenotype and increased spine density. (sciencemag.org)
  • Primary Antibodies are guaranteed for 1 year from date of receipt. (novusbio.com)
  • In BD patients, antibodies to cofilin-2 may react with cofilin-1 through the amino acid homology. (hsp90inhibitors.com)
  • Through a focused si RNA screen targeting genes involved in membrane trafficking, we discovered that mutant SOD 1 aggregates, like viruses, exploit cofilin‐1 to remodel cortical actin and enter cells. (embopress.org)
  • Our findings are consistent with a model suggesting a multi-functional role of the cortical actin in mediating chemokine coreceptor density, HIV-1 DNA synthesis and intracellular migration. (biomedcentral.com)
  • Interactions between the key factors involved in regulation of cortical actin are shown in the context of HIV-1 infection. (nih.gov)
  • is that HIV-induced CXCR4-dependent signaling triggers rapid polymerization and subsequent depolymerization of the cortical actin filaments (Fig. 1). (nih.gov)
  • A) Effect of RSV infection on cytoplasmic-nuclear localization of Prdx-1 and Prdx-4 in A549 cells by Western blotting. (asm.org)
  • In mammals, there are three members of the AC family, muscle-specific cofilin (cofilin 2), non-muscle cofilin (cofilin 1), and ADF. (ecmbio.com)
  • Western blotting further revealed that As2O3 treatment decreased the cytoplasmic cofilin‑1 level but increased its expression in the mitochondrion. (spandidos-publications.com)
  • Furthermore, we identify flotillin-1 (FLOT1) and histone H1 as downstream factors for cytoplasmic and nuclear pathway of S100A11, which are required for LASP1-S100A11 axis-mediated epithelial-mesenchymal transition and colorectal cancer progression. (antibodies-online.com)
  • We now show in a large array of unique in vitro and in vivo disease models that phosphorylated ERK1/2 (pERK1/2) binds to and activates cofilin-1 in LMNA cardiomyopathy. (deepdyve.com)
  • Interaction between the viral gp120 and cellular HIV co-receptor, CXCR4, during viral entry initiates signaling that activates cofilin, the main regulator of actin polymerization. (nih.gov)
  • We now show in a large array of unique in vitro and in vivo disease models that phosphorylated ERK1/2 (pERK1/2) binds to and activates cofilin-1 in LMNA cardiomyopa- thy. (readkong.com)
  • Actin polymerization is an essential mechanism of chemotactic cell motility induced by chemokines [18], and has been documented for CXCR4-dependent T cell chemotaxis in response to SDF-1 or gp120 stimulation [19,20]. (nih.gov)
  • HIV-1 Nef interferes with host cell motility by deregulation of Cofilin. (semanticscholar.org)
  • Our data thus identify PKD as a central regulator of the cofilin signaling network via direct phosphorylation and regulation of SSH1L. (aacrjournals.org)
  • We now show that BCR-induced MTOC polarization requires the Rap1 GTPase (which has two isoforms, Rap1a and Rap1b), an evolutionarily conserved regulator of cell polarity, as well as cofilin-1, an actin-severing protein that is regulated by Rap1. (biologists.org)
  • One group reports that reelin signaling leads to serine3-phosphorylation of cofilin-1, and this interaction may play a role in the reelin-related regulation of neuronal migration. (wikipedia.org)
  • Deficiency of cofilin-2 may result in reduced depolymerization of actin filaments, causing their accumulation in nemaline bodies, minicores, and, possibly concentric laminated bodies. (wikipedia.org)
  • This leads to depolymerization of F-actin, releasing HIV-1 reverse transcription complex and promoting its translocation towards the nucleus. (nih.gov)
  • Here we identify a pathway for the regulation of cofilin, a ubiquitous actin-binding protein that is essential for effective depolymerization of actin filaments. (nih.gov)
  • Cofilin controls reversible actin polymerization and depolymerization in a pH-sensitive manner. (genscript.com)
  • These biochemical differences reflect variations in the cellular expression between isoforms, where ADF and cofilin-1 are mainly expressed in tissues with higher actin turnover. (frontiersin.org)
  • Klemke M, Wabnitz GH, Funke F, Funk B, Kirchgessner H, Samstag Y. Oxidation of cofilin mediates T cell hyporesponsiveness under oxidative stress conditions. (springer.com)
  • Bernstein BW, Bamburg JR. ADF/cofilin: a functional node in cell biology. (springer.com)
  • This phosphorylation disrupts cofilin binding to actin in vitro and in vivo. (ecmbio.com)
  • Li M, Yin J, Mao N, Pan L. Upregulation of phosphorylated cofilin 1 correlates with taxol resistance in human ovarian cancer in vitro and in vivo. (springer.com)
  • It is reported that cofilins inhibit selleck screening library interactions between actin and myosin and between actin and tropomyosin (32), and mutation of cofilin-2 genes resulted in nemaline myopathy (33). (hsp90inhibitors.com)
  • Subsequently, the effects of cofilin‑1 on As2O3‑induced apoptosis in NB4‑R1 cells were further investigated. (spandidos-publications.com)
  • This suggested that As2O3 can induce the transfer of cofilin‑1 from the cytoplasm to mitochondria and trigger the release of mitochondrial cytochrome C in NB4‑R1 cells. (spandidos-publications.com)
  • In line with this, we show that the loss of PKD decreases cofilin phosphorylation, induces a more spread cell morphology, and stimulates chemotactic migration of breast cancer cells in an SSHL1-dependent fashion. (aacrjournals.org)
  • Mutant SOD1 protein aggregates use cofilin‐1 to remodel actin and invade host cells. (embopress.org)
  • Tau and α‐synuclein aggregates also exploit RHO GTPase signaling to cofilin‐1 to enter cells. (embopress.org)
  • Here we focused on the roles of ADF and cofilin-1 individually in the development of polarized migration of rat mammary adenocarcinoma MTLn3 cells, which express nearly equal amounts of each protein. (duhnnae.com)
  • ResultsEither ADF KD or cofilin KD caused cell elongation, a reduction in cell area, a decreased ability to form invadopodia, and a decreased percentage of polarized cells after 180 s of epidermal growth factor stimulation. (duhnnae.com)
  • Moreover, ADF KD or cofilin KD increased the rate of cell migration and the time of lamellipodia protrusion but through different mechanisms: lamellipodia protrude more frequently in ADF KD cells and are more persistent in cofilin KD cells. (duhnnae.com)
  • ADF KD cells showed a significant increase in F-actin aggregates, whereas cofilin KD cells showed a significant increase in prominent F-actin bundles and increased cell adhesion. (duhnnae.com)
  • Focal adhesion area and cell adhesion in cofilin KD cells were returned to control levels by expressing exogenous cofilin but not ADF. (duhnnae.com)
  • Studies with a cold sensitive mutant of Chinese hamster ovary cells," Experimental Cell Research , vol. 107, no. 1, pp. 89-94, 1977. (hindawi.com)
  • C3H10T1/2 pluripotent stem cells were plated at low density, cultured without or with BMP2 or BMP4 until postconfluence, and then subjected to the adipocyte differentiation protocol (MDI: adipocyte differentiation cocktail, 1 μ m dexamethasone, 1 μg/ml insulin and 0.5 m m isobutylmethyl-xanthine). (mcponline.org)
  • Validation of peroxiredoxin (Prdx) 1 and Prdx-4 expression in A549 cells by Western immunoblotting. (asm.org)
  • Cells were transfected with 100 nM scrambled (Con), Prdx-1, Prdx-4, or Prdx-1 plus Prdx-4 siRNA. (asm.org)
  • A) After 72 h, cells were harvested and Prdx-1 and Prdx-4 were detected in whole-cell extract (WCE) by Western immunoblotting. (asm.org)
  • B) A549 cells were transfected with either scrambled, Prdx-1, Prdx-4, or Prdx-1 plus Prdx-4 siRNA for 72 h, followed by no infection or RSV infection for 15 h. (asm.org)
  • ROS production in A549 cells lacking Prdx-1, Prdx-4, or both. (asm.org)
  • However, the use of these cells appears to only provide good medium-term outcomes attributed to the ageing transplanted cells, which is expected of any adult somatic cells [ 1 ]. (medsci.org)
  • In cells, a protein called cofilin-1 appears to inhibit glucocorticoid function. (scienceblog.com)
  • Western blot analysis of extracts from COS-7 and HeLa cells using Cofilin (D3F9) XP ® Rabbit mAb (HRP Conjugate). (cellsignal.com)
  • 1 , 2 Since DNDs have been demonstrated to be highly biocompatible, non-cytotoxic, and easily penetrable to mammalian cells, they represent excellent tools for biotechnological and medical applications. (dovepress.com)
  • Cleavage of IPS-1 in cells infected with human rhinovirus. (semanticscholar.org)
  • 1. What are the changes that occur in the cells that occur during tumour progression? (brainscape.com)
  • Studies from invertebrate systems have defined the concept of an equivalence group, where small clusters of lineage related cells are determined by a combination of inductive and intrinsic signals to adopt individual fates [1] - [6] . (prolekare.cz)