Palmitates: Salts and esters of the 16-carbon saturated monocarboxylic acid--palmitic acid.Palmitic Acid: A common saturated fatty acid found in fats and waxes including olive oil, palm oil, and body lipids.Insulin-Secreting Cells: A type of pancreatic cell representing about 50-80% of the islet cells. Beta cells secrete INSULIN.Diabetes Mellitus, Type 1: A subtype of DIABETES MELLITUS that is characterized by INSULIN deficiency. It is manifested by the sudden onset of severe HYPERGLYCEMIA, rapid progression to DIABETIC KETOACIDOSIS, and DEATH unless treated with insulin. The disease may occur at any age, but is most common in childhood or adolescence.Palmitic Acids: A group of 16-carbon fatty acids that contain no double bonds.Islets of Langerhans: Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.Hemizygote: An individual having only one allele at a given locus because of the loss of the other allele through a mutation (e.g., CHROMOSOME DELETION).Autophagy: The segregation and degradation of damaged or unwanted cytoplasmic constituents by autophagic vacuoles (cytolysosomes) composed of LYSOSOMES containing cellular components in the process of digestion; it plays an important role in BIOLOGICAL METAMORPHOSIS of amphibians, in the removal of bone by osteoclasts, and in the degradation of normal cell components in nutritional deficiency states.HIV-1: The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.Macrophages: The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)HIV Envelope Protein gp120: External envelope protein of the human immunodeficiency virus which is encoded by the HIV env gene. It has a molecular weight of 120 kDa and contains numerous glycosylation sites. Gp120 binds to cells expressing CD4 cell-surface antigens, most notably T4-lymphocytes and monocytes/macrophages. Gp120 has been shown to interfere with the normal function of CD4 and is at least partly responsible for the cytopathic effect of HIV.HIV-2: An HIV species related to HIV-1 but carrying different antigenic components and with differing nucleic acid composition. It shares serologic reactivity and sequence homology with the simian Lentivirus SIMIAN IMMUNODEFICIENCY VIRUS and infects only T4-lymphocytes expressing the CD4 phenotypic marker.CD4-Positive T-Lymphocytes: A critical subpopulation of T-lymphocytes involved in the induction of most immunological functions. The HIV virus has selective tropism for the T4 cell which expresses the CD4 phenotypic marker, a receptor for HIV. In fact, the key element in the profound immunosuppression seen in HIV infection is the depletion of this subset of T-lymphocytes.HIV Envelope Protein gp41: Transmembrane envelope protein of the HUMAN IMMUNODEFICIENCY VIRUS which is encoded by the HIV env gene. It has a molecular weight of 41,000 and is glycosylated. The N-terminal part of gp41 is thought to be involved in CELL FUSION with the CD4 ANTIGENS of T4 LYMPHOCYTES, leading to syncytial formation. Gp41 is one of the most common HIV antigens detected by IMMUNOBLOTTING.National Institute of Allergy and Infectious Diseases (U.S.): Component of the NATIONAL INSTITUTES OF HEALTH. It conducts and supports basic and applied research to better understand, treat, and ultimately prevent infectious, immunologic, and allergic diseases. It was established in 1948.Oxymetholone: A synthetic hormone with anabolic and androgenic properties. It is used mainly in the treatment of anemias. According to the Fourth Annual Report on Carcinogens (NTP 85-002), this compound may reasonably be anticipated to be a carcinogen. (From Merck Index, 11th ed)Leukemia, Myeloid, Acute: Clonal expansion of myeloid blasts in bone marrow, blood, and other tissue. Myeloid leukemias develop from changes in cells that normally produce NEUTROPHILS; BASOPHILS; EOSINOPHILS; and MONOCYTES.National Institute of General Medical Sciences (U.S.): Component of the NATIONAL INSTITUTES OF HEALTH. It conducts and supports basic biomedical research that is not targeted to specific diseases and funds studies on genes, proteins, and cells, as well as on fundamental processes like communication within and between cells and metabolism. It was established in 1962.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Biomedical Research: Research that involves the application of the natural sciences, especially biology and physiology, to medicine.NIH 3T3 Cells: A continuous cell line of high contact-inhibition established from NIH Swiss mouse embryo cultures. The cells are useful for DNA transfection and transformation studies. (From ATCC [Internet]. Virginia: American Type Culture Collection; c2002 [cited 2002 Sept 26]. Available from http://www.atcc.org/)Dendritic Cells: Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).Myelopoiesis: Formation of MYELOID CELLS from the pluripotent HEMATOPOIETIC STEM CELLS in the BONE MARROW via MYELOID STEM CELLS. Myelopoiesis generally refers to the production of leukocytes in blood, such as MONOCYTES and GRANULOCYTES. This process also produces precursor cells for MACROPHAGE and DENDRITIC CELLS found in the lymphoid tissue.Cell Differentiation: Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.Point Mutation: A mutation caused by the substitution of one nucleotide for another. This results in the DNA molecule having a change in a single base pair.Hematopoiesis: The development and formation of various types of BLOOD CELLS. Hematopoiesis can take place in the BONE MARROW (medullary) or outside the bone marrow (HEMATOPOIESIS, EXTRAMEDULLARY).Bone Marrow Cells: Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.Hematopoietic Stem Cells: Progenitor cells from which all blood cells derive.Glycyrrhizic Acid: A widely used anti-inflammatory agent isolated from the licorice root. It is metabolized to GLYCYRRHETINIC ACID, which inhibits 11-BETA-HYDROXYSTEROID DEHYDROGENASES and other enzymes involved in the metabolism of CORTICOSTEROIDS. Therefore, glycyrrhizic acid, which is the main and sweet component of licorice, has been investigated for its ability to cause hypermineralocorticoidism with sodium retention and potassium loss, edema, increased blood pressure, as well as depression of the renin-angiotensin-aldosterone system.Glycyrrhetinic Acid: An oleanolic acid from GLYCYRRHIZA that has some antiallergic, antibacterial, and antiviral properties. It is used topically for allergic or infectious skin inflammation and orally for its aldosterone effects in electrolyte regulation.Sarcoma, YoshidaNeutrophils: Granular leukocytes having a nucleus with three to five lobes connected by slender threads of chromatin, and cytoplasm containing fine inconspicuous granules and stainable by neutral dyes.Glycyrrhiza: A genus of leguminous herbs or shrubs whose roots yield GLYCYRRHETINIC ACID and its derivative, CARBENOXOLONE.Glycyrrhiza uralensis: A plant species of the family FABACEAE.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Publications: Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)Research: Critical and exhaustive investigation or experimentation, having for its aim the discovery of new facts and their correct interpretation, the revision of accepted conclusions, theories, or laws in the light of newly discovered facts, or the practical application of such new or revised conclusions, theories, or laws. (Webster, 3d ed)Malaysia: A parliamentary democracy with a constitutional monarch in southeast Asia, consisting of 11 states (West Malaysia) on the Malay Peninsula and two states (East Malaysia) on the island of BORNEO. It is also called the Federation of Malaysia. Its capital is Kuala Lumpur. Before 1963 it was the Union of Malaya. It reorganized in 1948 as the Federation of Malaya, becoming independent from British Malaya in 1957 and becoming Malaysia in 1963 as a federation of Malaya, Sabah, Sarawak, and Singapore (which seceded in 1965). The form Malay- probably derives from the Tamil malay, mountain, with reference to its geography. (From Webster's New Geographical Dictionary, 1988, p715 & Room, Brewer's Dictionary of Names, 1992, p329)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Coculture Techniques: A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.Skin: The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.Neoplasms: New abnormal growth of tissue. Malignant neoplasms show a greater degree of anaplasia and have the properties of invasion and metastasis, compared to benign neoplasms.Fibroblasts: Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.Carcinoma, Squamous Cell: A carcinoma derived from stratified SQUAMOUS EPITHELIAL CELLS. It may also occur in sites where glandular or columnar epithelium is normally present. (From Stedman, 25th ed)JapanCollagen: A polypeptide substance comprising about one third of the total protein in mammalian organisms. It is the main constituent of SKIN; CONNECTIVE TISSUE; and the organic substance of bones (BONE AND BONES) and teeth (TOOTH).Kupffer Cells: Specialized phagocytic cells of the MONONUCLEAR PHAGOCYTE SYSTEM found on the luminal surface of the hepatic sinusoids. They filter bacteria and small foreign proteins out of the blood, and dispose of worn out red blood cells.Nitric Oxide: A free radical gas produced endogenously by a variety of mammalian cells, synthesized from ARGININE by NITRIC OXIDE SYNTHASE. Nitric oxide is one of the ENDOTHELIUM-DEPENDENT RELAXING FACTORS released by the vascular endothelium and mediates VASODILATION. It also inhibits platelet aggregation, induces disaggregation of aggregated platelets, and inhibits platelet adhesion to the vascular endothelium. Nitric oxide activates cytosolic GUANYLATE CYCLASE and thus elevates intracellular levels of CYCLIC GMP.Cells, Cultured: Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.Nitric Oxide Synthase: An NADPH-dependent enzyme that catalyzes the conversion of L-ARGININE and OXYGEN to produce CITRULLINE and NITRIC OXIDE.Liver Neoplasms, Experimental: Experimentally induced tumors of the LIVER.Liver: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (1/7795)

Interleukin-12 (IL-12) is a potent antitumor cytokine, which induces and enhances the activity of natural killer (NK) cells, lymphokine activated killer (LAK) cells and cytotoxic T lymphocytes (CTL). IL-12 also stimulates IFN-gamma production from both T cells and NK cells. In this study, we transfected methylcholanthrene-induced fibrosarcoma (MCA-D) with TNF gene and investigated the therapeutic effect of TNF gene-transduced cancer vaccine and whether the vaccination effect is enhanced by systemic administration of recombinant IL-12 (rIL-12), in a murine model. TNF gene-transduced cancer vaccine or systemic administration of rIL-12 showed slight or moderate inhibition of pre-established tumor. However, simultaneous application of the vaccine and rIL-12 resulted in complete eradication. The cytotoxicity of CTL against parental tumor cells was enhanced with the combination of the vaccine and rIL-12, and IFN-gamma production from spleen cells also increased synergistically. Our findings show that synergistic enhancement of CTL activity and IFN-gamma production could play an important role in the antitumor effect of combination therapy using TNF gene-transduced cancer vaccine and rIL-12.  (+info)

Regulation of neurotrophin-3 expression by epithelial-mesenchymal interactions: the role of Wnt factors. (2/7795)

Neurotrophins regulate survival, axonal growth, and target innervation of sensory and other neurons. Neurotrophin-3 (NT-3) is expressed specifically in cells adjacent to extending axons of dorsal root ganglia neurons, and its absence results in loss of most of these neurons before their axons reach their targets. However, axons are not required for NT-3 expression in limbs; instead, local signals from ectoderm induce NT-3 expression in adjacent mesenchyme. Wnt factors expressed in limb ectoderm induce NT-3 in the underlying mesenchyme. Thus, epithelial-mesenchymal interactions mediated by Wnt factors control NT-3 expression and may regulate axonal growth and guidance.  (+info)

Reciprocal control of T helper cell and dendritic cell differentiation. (3/7795)

It is not known whether subsets of dendritic cells provide different cytokine microenvironments that determine the differentiation of either type-1 T helper (TH1) or TH2 cells. Human monocyte (pDC1)-derived dendritic cells (DC1) were found to induce TH1 differentiation, whereas dendritic cells (DC2) derived from CD4+CD3-CD11c- plasmacytoid cells (pDC2) induced TH2 differentiation by use of a mechanism unaffected by interleukin-4 (IL-4) or IL-12. The TH2 cytokine IL-4 enhanced DC1 maturation and killed pDC2, an effect potentiated by IL-10 but blocked by CD40 ligand and interferon-gamma. Thus, a negative feedback loop from the mature T helper cells may selectively inhibit prolonged TH1 or TH2 responses by regulating survival of the appropriate dendritic cell subset.  (+info)

Endothelial cells modulate the proliferation of mural cell precursors via platelet-derived growth factor-BB and heterotypic cell contact. (4/7795)

Embryological data suggest that endothelial cells (ECs) direct the recruitment and differentiation of mural cell precursors. We have developed in vitro coculture systems to model some of these events and have shown that ECs direct the migration of undifferentiated mesenchymal cells (10T1/2 cells) and induce their differentiation toward a smooth muscle cell/pericyte lineage. The present study was undertaken to investigate cell proliferation in these cocultures. ECs and 10T1/2 cells were cocultured in an underagarose assay in the absence of contact. There was a 2-fold increase in bromodeoxyuridine labeling of 10T1/2 cells in response to ECs, which was completely inhibited by the inclusion of neutralizing antiserum against platelet-derived growth factor (PDGF)-B. Antisera against PDGF-A, basic fibroblast growth factor, or transforming growth factor (TGF)-beta had no effect on EC-stimulated 10T1/2 cell proliferation. EC proliferation was not influenced by coculture with 10T1/2 cells in the absence of contact. The cells were then cocultured so that contact was permitted. Double labeling and fluorescence-activated cell sorter analysis revealed that ECs and 10T1/2 cells were growth-inhibited by 43% and 47%, respectively. Conditioned media from contacting EC-10T1/2 cell cocultures inhibited the growth of both cell types by 61% and 48%, respectively. Although we have previously shown a role for TGF-beta in coculture-induced mural cell differentiation, growth inhibition resulting from contacting cocultures or conditioned media was not suppressed by the presence of neutralizing antiserum against TGF-beta. Furthermore, the decreased proliferation of 10T1/2 cells in the direct cocultures could not be attributed to downregulation of the PDGF-B in ECs or the PDGF receptor-beta in the 10T1/2 cells. Our data suggest that modulation of proliferation occurs during EC recruitment of mesenchymal cells and that heterotypic cell-cell contact and soluble factors play a role in growth control during vessel assembly.  (+info)

Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis. (5/7795)

OBJECTIVE: To investigate the features of synovial stromal cells established from patients with rheumatoid arthritis (RA), and to define these cells as nurse cells. METHODS: Synovial nurse-like stromal cell lines (RA-SNCs) were established from patients with RA. These cell lines were examined for morphology, pseudoemperipolesis activity, cell surface markers, and cytokine production. The interaction between these RA-SNCs and a synovial tissue B cell clone was also examined. RESULTS: RA-SNCs had nurse cell activity. They spontaneously produced interleukin-6 (IL-6), IL-8, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. Furthermore, they produced IL-1beta and tumor necrosis factor alpha and expressed higher levels of the other cytokines after coculture with the B cell clone. Proliferation and Ig production by the B cell clone were dependent on direct contact with RA-SNCs. CONCLUSION: These results indicate that the RA-SNCs were nurse cells. The findings suggest that RA-SNCs may play an important role in the pathogenesis of RA by producing large amounts of cytokines and maintaining infiltrating lymphocytes.  (+info)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (6/7795)

Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease.  (+info)

In vitro hematopoietic and endothelial cell development from cells expressing TEK receptor in murine aorta-gonad-mesonephros region. (7/7795)

Recent studies have shown that long-term repopulating hematopoietic stem cells (HSCs) first appear in the aorta-gonad-mesonephros (AGM) region. Our immunohistochemistry study showed that TEK+ cells existed in the AGM region. Approximately 5% of AGM cells were TEK+, and most of these were CD34(+) and c-Kit+. We then established a coculture system of AGM cells using a stromal cell line, OP9, which is deficient in macrophage colony-stimulating factor (M-CSF). With this system, we showed that AGM cells at 10.5 days postcoitum (dpc) differentiated and proliferated into both hematopoietic and endothelial cells. Proliferating hematopoietic cells contained a significant number of colony-forming cells in culture (CFU-C) and in spleen (CFU-S). Among primary AGM cells at 10.5 dpc, sorted TEK+ AGM cells generated hematopoietic cells and platelet endothelial cell adhesion molecule (PECAM)-1(+) endothelial cells on the OP9 stromal layer, while TEK- cells did not. When a ligand for TEK, angiopoietin-1, was added to the single-cell culture of AGM, endothelial cell growth was detected in the wells where hematopoietic colonies grew. Although the incidence was still low (1/135), we showed that single TEK+ cells generated hematopoietic cells and endothelial cells simultaneously, using a single-cell deposition system. This in vitro coculture system shows that the TEK+ fraction of primary AGM cells is a candidate for hemangioblasts, which can differentiate into both hematopoietic cells and endothelial cells.  (+info)

Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8(+) T cells resulting in a failure to lyse tumor cells. (8/7795)

Dendritic cells (DC) are critically involved in the initiation of primary immune processes, including tumor rejection. In our study, we investigated the effect of interleukin-10 (IL-10)-treated human DC on the properties of CD8(+) T cells that are known to be essential for the destruction of tumor cells. We show that IL-10-pretreatment of DC not only reduces their allostimulatory capacity, but also induces a state of alloantigen-specific anergy in both primed and naive (CD45RA+) CD8(+) T cells. To investigate the influence of IL-10-treated DC on melanoma-associated antigen-specific T cells, we generated a tyrosinase-specific CD8(+) T-cell line by several rounds of stimulation with the specific antigen. After coculture with IL-10-treated DC, restimulation of the T-cell line with untreated, antigen-pulsed DC demonstrated peptide-specific anergy in the tyrosinase-specific T cells. Addition of IL-2 to the anergic T cells reversed the state of both alloantigen- or peptide-specific anergy. In contrast to optimally stimulated CD8(+) T cells, anergic tyrosinase-specific CD8(+) T cells, after coculture with peptide-pulsed IL-10-treated DC, failed to lyse an HLA-A2-positive and tyrosinase-expressing melanoma cell line. Thus, our data demonstrate that IL-10-treated DC induce an antigen-specific anergy in cytotoxic CD8(+) T cells, a process that might be a mechanism of tumors to inhibit immune surveillance by converting DC into tolerogenic antigen-presenting cells.  (+info)

*Autologous endometrial coculture

... is a technique of assisted reproductive technology. It involves placing a patient's fertilized ... Coculture can be an effective treatment for patients who have failed previous IVF cycles or who have poor embryo quality. A ... A typical Coculture cycle consists of the following steps: 1. Once a patient has been deemed an appropriate candidate for the ... The risks of Coculture are minimal. The procedure has been performed in over 1000 patients with no reported detrimental effects ...

*Jacques Cohen

At the same clinic, he developed methods to treat male factor infertility using in vitro fertilization (IVF). He also co- ... He developed a precursor technique of Intracytoplasmic Sperm Injection (ICSI), which is now used for treatment of nearly all ... 1992). "Evaluation of Vero cell co-culture system for mouse embryos in various media". Human Reproduction. 7 (2): 276-80. PMID ... Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. Intracytoplasmic ...

*Shan Ratnam

He was a world authority on sex change operations, developing new techniques for transsexualism, embryo replacement, in vitro ... He contributed to the world's first microinjection baby via human ampullary coculture[clarification needed] in 1991. Ratnam ... and how he used cadavers to come up with his technique of sexual reassignment surgery. It also shows his assistant, Dr Lim, ...

*Embryonic stem cell

The techniques are now used by many pregnant women and prospective parents, especially those couples with a history of genetic ... is co-cultured with a serum containing necessary signals, or is grafted in a three-dimensional scaffold to result. Embryonic ... It is a common technique to use mouse cells and other animal cells to maintain the pluripotency of actively dividing stem cells ... Weiss, Rick (2007-12-07). "Scientists Cure Mice Of Sickle Cell Using Stem Cell Technique: New Approach Is From Skin, Not ...

*Amniotic stem cells

A variety of techniques has been developed for the isolation and culturing of amniotic stem cells. One of the more common ... Co-culturing, i.e. mixing cells and plating them together, of human amniotic stem cells with neonatal rat ventricular myocytes ... Mesenchymal stem cells (MSCs) are highly abundant in the amniotic fluid and several techniques have been described for their ... In general, these types of techniques are considered to be potentially significant but further investigations are required. ...

*Legionella anisa

Genome-based analytical techniques may prove especially useful for L. anisa, as a study of isolates from various locations in ... However, some research suggests L. anisa may require a co-culture method that accounts for the close relationship with amoebae ... In addition, such techniques greatly reduce the time required to obtain results. Infections may be asymptomatic, and are ... "Isolation of Legionella anisa Using an Amoebic Coculture Procedure". J Clin Microbiol. 39 (1): 365-6. doi:10.1128/JCM.39.1.365- ...

*3D cell culture

In general, there are two types of 3D culture methods: scaffold techniques and scaffold-free techniques. Scaffold techniques ... Spheroids co-cultured with tumor and healthy cells were used to simulate how cancerous cells interact with normal cells. ... Scaffold free techniques employ another approach independent from the use scaffold. Scaffold-free methods include e.g. the use ... These techniques have been applied to for in vitro disease models used to evaluate cellular responses to pharmaceutical ...

*Induced stem cells

The technique can be applied to a large number of cells to produce stem cells for medical purposes on an industrial scale. ... While initial studies revealed that co-culturing epithelial cells with Swiss 3T3 cells J2 was essential for CRC induction, with ... The technique enables researchers to search large libraries of antibodies and quickly select the ones with a desired biological ... Three techniques are widely recognized: Transplantation of nuclei taken from somatic cells into an oocyte (egg cell) lacking ...

*E. coli long-term evolution experiment

Clones of the S and L types could co-exist stably in co-culture with each other, indicating they occupied distinct niches in ... A later study by Leiby and Marx that used more advanced techniques showed that much of the decay Cooper and Lenski had ... and as technology and methodological techniques have advanced. The use of E. coli as the experimental organism has allowed many ... by Eric Quandt in the lab of Jeffrey Barrick at the University of Texas at Austin described the application of a new technique ...

*Exometabolomics

Recently, exometabolomics has been used to design co-culture systems. Because the analysis of extracellular metabolites allows ... Exometabolomic techniques have been used in the following fields: Metabolite utilization to annotate function of unknown genes ... in combination with other techniques, for early recognition of outbreak and strain characterization Studying aging with C. ... "Liquid chromatography-mass spectrometry for metabolic footprinting of co-cultures of lactic and propionic acid bacteria". ...

*Adoptive cell transfer

In 2016, researchers developed a technique that used cancer cells' RNA to produce T cells and an immune response. They encased ... They then produce pure cultures of lymphocytes that can be tested for reactivity against other tumors, in coculture assays. ... As of 2015 the technique had expanded to treat cervical cancer, lymphoma, leukemia, bile duct cancer and neuroblastoma and in ...

*In vitro maturation

This technique is also used in sheep, pigs and other animals. See In animals. Oocytes are classified depending on their ... Kannan S.; Mehta A.; Simha V.; Reddy O.; Kaur B.; Onteru S.; Singh D. (2014). "Photoinduction of granulosa cell and oocyte co-culture ... Wani, N.A; Wani, G.M; Khan, M.Z; Salahudin, S. "Effect of oocyte harvesting techniques on in vitro maturation and in vitro ... The best oocytes are chosen to be matured in the hope of then being implanted using in vitro fertilisation techniques. The ...

*Chlorella vulgaris

Different growth techniques have been developed. They exploit the autotrophic, heterotrophic or mixotrophic properties of C. ... Gonzalez, L. E., & Bashan, Y. (2000). Increased growth of the microalga chlorella vulgariswhen coimmobilized and cocultured in ... Other techniques exist as well, such as flocculation, filtration, flotation. C. vulgaris is seen as a promising source of ... differ depending on the technique used to create biomass and can be therefore targeted. Under more hostile conditions, the ...

*Myelinogenesis

Distinct Stages of Myelination Regulated by Y-Secretase and Astrocytes in a Rapidly Myelinating CNS Coculture System. 555-569 ... and mechanistic function of myelinogenesis has traditionally been studied using ultrastructure and biochemical techniques in ...

*Radiobiology evidence for protons and HZE nuclei

More recently, however, studies in 3D human coculture are proving to be an effective method with which to study cancer risks in ... Novel multicolor fluorescence painting techniques of human chromosomes have clearly demonstrated that high-LET α-particles and ...

*Acidithiobacillus thiooxidans

This technique has progressed steadily in the past 20 years by taking advantage of bacteria such as A. thiooxidans. Biomining ... A. thiooxidans is incapable of oxidizing iron or pyrite, but it has been shown to grow on sulfur from pyrite when cocultured ... A. thiooxidans is used in the mining technique known as bioleaching, where metals are extracted from their ores through the ... Bioleaching is a mining technique in which metals are extracted from their insoluble ores through the use of living organisms ...

*Organoid

The technique for growing organoids has rapidly improved since the early 2010s, and it was named by The Scientist as one of the ... Thymic organoids seem to successfully recapitulate the thymus's function, as co-culturing human hematopoietic or bone marrow ... These advancements in PSC fate direction, coupled with the advancements in 3D culturing techniques allowed for the creation of ...

*Cell culture

These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid ... Duell BL, Cripps AW, Schembri MA, Ulett GC (2011). "Epithelial cell coculture models for studying infectious diseases: benefits ... This technique is known as two-dimensional (2D) cell culture, and was first developed by Wilhelm Roux who, in 1885, removed a ... The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the ...

*Bartonellosis

Insect-based liquid media supports the growth and co-culturing of at least seven Bartonella species, reduces bacterial ... Lynch, T (2011). "Combining culture techniques for Bartonella: the best of both worlds". J Clin Microbiol. 49: 1363-8. doi: ...

*Intensive animal farming

The co-cultured species should be more than just biofilters; they should also be harvestable crops of commercial value. A ... There are differences in the way factory farming techniques are practiced around the world. There is a continuing debate over ... This is one potential distinction from the age-old practice of aquatic polyculture, which could simply be the co-culture of ... including the development of modern broiler rearing techniques, the adoption of the Cornish Cross broiler, the use of laying ...

*Mycoremediation

This study demonstrated that both the monoculture extracts of the native strain T. maxima and its co-culture with P. carneus ... recovery of gold from electronic waste using mycofiltration techniques.[1].[better source needed] Fungi are amongst the primary ... Chan-Cupul, Wilberth; Heredia-Abarca, Gabriela; Rodríguez-Vázquez, Refugio (2016). "Atrazine degradation by fungal co-culture ... a prospective environmental friendly technique of bioseparation and dewatering of domestic wastewater sludge". Environmental ...

*Proteases in angiogenesis

Co-cultures consisting of pericytes and endothelial cells induce the expression of TIMP-3 by pericytes, while endothelial cells ... Examination of cathepsin activity by using chemical probes and in vivo imaging techniques demonstrated an increase in cathepsin ...

*Index of oncology articles

... co-culture - co-trimoxazole - coactivated T cell - cobalt 60 - Cockayne syndrome - coenzyme Q10 - cohort study - COL-3 - cold ... relaxation technique - remission induction therapy - remote brachytherapy - renal cell cancer - renal collecting tubule - renal ...
cell-cell contact in transwell systems - posted in Tissue and Cell Culture: I would like to conduct transwell experiments with two cell types and wants to investigate the effect of cell-cell interaction between these 2 cell types. Do anyone have protocol of doing co-culture of these cells in the transwell system (ie. cell type 1 on the upper surface of the insert; while cell type 2 on the lower surface of the insert). Thank you very much.
Muscle regeneration involves the coordination of myogenesis and revascularization to restore proper muscle function. Myogenesis is driven by resident stem cells termed satellite cells (SC), whereas angiogenesis arises from endothelial cells and perivascular cells of preexisting vascular segments and the collateral vasculature. Communication between myogenic and angiogenic cells seems plausible, especially given the number of growth factors produced by SC. To characterize these interactions, we developed an in vitro coculture model composed of rat skeletal muscle SC and microvascular fragments (MVF). In this system, isolated epididymal MVF suspended in collagen gel are cultured over a rat SC monolayer culture. In the presence of SC, MVF exhibit greater indices of angiogenesis than MVF cultured alone. A positive dose-dependent effect of SC conditioned medium (CM) on MVF growth was observed, suggesting that SC secrete soluble-acting growth factor(s). Next, we specifically blocked VEGF action in SC ...
Results ICAM-1 expression was significantly up-regulated upon the introduction of TNF-α under all conditions in HUVEC (figure 1). However, baseline expression was increased when co-cultured with both NHDF (2.0 vs 1.3, p,0.001) and HUASMC (6.5 vs 1.3, p,0.001). This meant that ICAM-1 expression at 12 hours was also significantly higher in co-culture with NHDF (8.3 vs 5.2, p,0.001) and HUASMC (11.0 vs 5.2, p,0.001). Moreover, there was a moderate relationship between HUVEC ICAM-1 expression and the cell ratio when in co-culture with NHDF, where decreasing NHDF resulted in decreased ICAM-1 in HUVEC (R2=0.45).. TNF-α caused an increase in ICAM-1 expression in NHDF under monoculture conditions (Fig. 2); this up-regulation was significantly reduced in co-culture conditions with HUVEC (1.7 vs 5.1, p,0.001). A similar trend was observed when in co-culture with HUASMC (2.7 vs 5.1, p,0.001), except the baseline expression of ICAM-1 was also increased (2.8 vs 1.1, p,0.001).. Constitutive production of ...
TY - JOUR. T1 - Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction. AU - Che, Zhong Min. AU - Jung, Tae Heob. AU - Choi, Jung Hee. AU - Yoon, Do Jun. AU - Jeong, Hyun Jeong. AU - Lee, Eun Ju. AU - Kim, Jin. PY - 2006/7/21. Y1 - 2006/7/21. N2 - The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. ...
Formulations comprising combinations of APCs and tumor cells and APCs and virally infected cells are disclosed. These formulations generally comprise hybridoma of at least one antigen presenting cell
Cell culture inserts were seeded with 1x105 cells in 250 uL of medium with 0. 1% FBS. Un coated inserts had been utilised for migration assays whereas inserts
Trans-Lux Corp., a tmaker of digital LED and LCD display screens, cites Trumps election victory and his tough talk on manufacturing as the main reason it is bringing production back to the U.S.
Endothelial vascular smooth muscle cell coculture assay for high throughput screening assays to identify antiangiogenic and other therapeutic molecules George A TruskeyDepartment of Biomedical Engineering, Duke University, Durham, NC, USAAbstract: Drug development for many diseases would be aided greatly by accurate in vitro model systems that replicate key elements of in vivo physiology. The recent development of coculture systems of endothelial cells and vascular smooth muscle cells can be extended to high-throughput systems for the identification of compounds for angiogenesis, vascular repair, and hypertension. In this review, the various coculture systems are reviewed, and biologic interactions between endothelial cells and vascular smooth muscle cells are discussed. Key considerations in the design of high-throughput systems are presented, and selected examples are discussed.Keywords: endothelium, vascular smooth muscle cells, angiogenesis, microfluidics
In vitro coculture systems are at the forefront of molecular research due to their increased ability for cell-cell communication. In this study, small airway epithelial cells (SAEC) and human microvascular endothelial cells (HMVEC) were grown separately in monoculture or together in an alveolar-capillary coculture and exposed to either dispersion media control or multi-walled carbon nanotubes (MWC
The recent clinical successes of immune-based cancer therapies highlight the utility of harnessing the cytotoxicity of CD8 + T cells to target tumors. While current immunotherapies are effective at supporting and enhancing the function of tumor-specific T cell responses, these cells remain subject to multiple tolerizing mechanisms in the tumor microenvironment which can ultimately limit their effectiveness. While the function of dedicated regulatory immune cell populations like MDSCs and Tregs are well-studied contributors to tumor tolerance, our studies suggest that additional tumor-mediated mechanisms exist.. Our previous studies indicated that tumor cell lines secreted a soluble factor that was capable of inducing phenotypic and functional changes in normal human T cells that closely resembled those associated with dysfunctional T cells present in the periphery and tumor microenvironment of cancer patients [16, 17]. Because these studies were based on in vitro co-culture experiments, we first ...
Video articles in JoVE about salmonella typhimurium include Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization, Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella, Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens, NF-κB-Dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells, Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria, In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration, Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses, Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways, Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers, Coincubation Assay for Quantifying
BACKGROUND. Prostate cancer (PCa) and bone cell interactions are critical in the metastatic phase. Kallikrein 4 (KLK4/hK4) is expressed in both PCa and mineralized tissues. We determined if KLK4/hK4 expression was associated with, and influenced by, the bone environment of metastatic PCa. METHODS. Immunohistochemistry, in vitro co-culture, cell migration, and attachment assays. RESULTS. hK4 was localized to tumor cells and osteoblasts in bone metastases. KLK4/hK4 increased in LNCaP and PC3 cells co-cultured with SaOs2 cells; SaOs2 KLK4/hK4 was unchanged. Co-culture did not affect cell proliferation but altered alkaline phosphatase activity/mRNA levels in SaOs2 cells. KLK4-transfected PC3 cells had increased migration towards SaOs2 conditioned medium and greater attachment to the bone-matrix proteins, collagens I and IV. CONCLUSIONS. hK4 expression and interaction with both tumor cells and osteoblasts suggests a role for hK4 in PCa bone metastasis. Whether this observation is unique to bone ...
Background P. gingivalis, a non-motile, rod-shaped, anaerobic, Gram-negative bacterium is one of the principal sources of periodontal disease. It possesses a number of potential virulence factors thought to be important in the disease process including 5 hemagglutinins (Hag). One of these is HagB. It is a well characterized nonfimbrial adhesin expressed on the surface of P. gingivalis. HagB is very pro-inflammatory and induces robust chemokine and cytokine responses in vitro and in vivo. Since the chemokine and cytokine responses seen from single cells grown in tissue culture often are not representative of the chemokine and cytokine profiles seen in clinical samples or biopsy specimens, we devised a co-culture model of keratinocytes, dendritic cells, and T-cells to test the hypothesis that chemokine and cytokine responses from co-cultured cells would be more representative of responses seen in clinical samples from individuals with periodontal disease than single cell models. Methods and materials HagB
Our present study has provided, for the first time to our knowledge, a systematic analysis of the inflammation-relevant gene expression in ECs and SMCs in their coculture under static condition and in response to shear stress. We demonstrated that SMCs cause an upregulation of proinflammatory gene expression in ECs located in close proximity and that shear stress acts as a negative regulator for these proinflammatory gene expression in ECs cocultured with SMCs. Under static coculture condition, DNA microarray technology identified 23 inflammation-relevant genes that exhibit significant changes (18 increases and 5 decreases) in their expression in ECs cocultured with SMCs, as compared with the control monocultured ECs. All 18 genes upregulated in ECs by the coculture encode products that serve proinflammatory and thrombogenic functions. These functions include: (1) mediation of leukocyte-EC interaction and signal transduction (ICAM-1, VCAM-1, CD44, and NK-4); (2) promotion of chemotaxis and ...
1. Using sterile forceps, pick up the coverslips containing neurons and place into the bottom of the co-culture plate. Be sure to keep the cells covered with medium. Replace the insert with the microglia coverslip. Alternatively, you could change the medium in the microglial culture completely, and do a wash with fresh medium, before replacing the medium, to get rid of the LPS. Then add the neurons ...
Lastly, we examined heterotypic interactions between tumor cells and cancer associated fibroblasts (CAFs) to understand the mechanism of resistance to lapatinib. Using a 3D co-culture model, we identified significant sub-type-specific changes in gene expression, metabolic, and therapeutic sensitivity profiles of breast cancer cells induced by CAFs. We identified JAK2/STAT3 pathway and CAF-secreted hyaluronan as major factors contributing to CAF-mediated protection. We also found that close spatial proximity to CAFs impacts therapeutic responses by affecting proliferation rates of cancer cells ...
In model 1, target CD4+ T cells (UC blood CD4+ T cells and a T cell line expressing CXCR4 and CD4.403) are cocultured with effector HEK cells with or without Env expression. In model 2, HEK/CD4.403/CXCR4 and HEK/CD4.403 cells are cocultured with effector T cells with or without Env expression. In model 3, target CD4+ T cells are cocultured with HIVNL4-3-infected or uninfected T cells. Expression of gp120 at the surface of HEK.Env, 8.E5, and HIVNL4-3-infected effector cells as well as parental HEK and CEM cells - used as negative controls - were analyzed by flow cytometry after incubation of the cells with PBS (white histograms) or with PBS containing anti-gp120 human polyclonal Ab (black histograms). Bound Ab was detected with a secondary FITC-labeled goat anti-human Ig. Expression analysis of the extracellular domains of CD4 and CXCR4 at the surface of target cells was performed after incubation of the cells with PBS (white histograms) or with anti-CD4 (black histograms) or anti-CXCR4 (gray ...
In the present study, we set out to determine the degrees of residual plasma viremia in a large number of infected individuals receiving ART and to determine immunological or virological parameters that may correlate with residual plasma viremia. Residual plasma viremia was determined in quadruplicate using an automated system to minimize quantitative errors often associated with the detection of extremely low levels of viral RNA. We have demonstrated detectable levels of residual plasma viremia (1-49 copies/mL) in the majority of study participants receiving ART in whom plasma viremia had been suppressed for extended periods of time, as measured by a standard clinical assay (with a limit of detection of 50 copies/mL). Furthermore, we found a correlation between the level of residual plasma viremia and the frequency of CD4+ T cells carrying HIV proviral DNA. Of note, due to the limited amounts of blood obtained from the study subjects, we could not conduct the quantitative coculture assays that ...
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IncuCyte® NeuroPrime Cell Kit offers a complete solution for kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat neurons & astrocytes.
Conjugation, the transfer of DNA by direct cell-to-cell contact, depends on the presence of a conjugative plasmid(is small, double-stranded DNA molecules
Hierarchical tissues composed of spheroid and fiber structures such as tumors, embryos and glomeruli widely exist in organisms. Methods have been developed to build spheroid and fiber structures, independently, as tissue models in vitro. However, it is still a challenge to print them simultaneously and integrated f
The underlying technique our lab is built upon is a hematopoetic/mesenchymal stem cell coculture. In this system we are able to acurately recreate the bone marrow niche in-vitro, manipulate the microenvironment, and investigate the effects of such manipulations on the niche using fluorescence imaging, flow cytometry, in situ immunohistochemistry, and serial transplants.. ...
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Ultrastructure of control and GGF-treated cocultures. Schwann cell-DRG neuron cocultures that had myelinated for 3 wk were maintained for an additional 3 d with
Purpose: : Evidences from literature shows that GITR-GITRL interaction plays important role in delivering co-stimulatory signals controlling regulatory T cells. Human GITR ligand has recently been shown to be constitutively expressed in retinal tissues. By expressing eYFP tagged human GITRL in human retinal pigment epithelial (RPE) cells, we investigated the biological significance of expression of GITRL on human ocular tissue. Methods: : Full length GITRL was amplified from a human ovarian cDNA library using GITRL specific primers and subcloned in a pEYFPC1 vector for expressing GITRL as an eYFP tagged protein in RPE cells. Using an RPE/T cell co-culture system we studied the invitro proliferative responses of purified CD3+ T cells or CD3+CD25-T cells co-cultured with lethally irradiated RPE cells in the presence or absence of anti-CD3 and anti CD28 antibody. For cytokine measurement, culture supernatants were collected from parallel RPE/T cell co-culture experiments. Results: : Both ...
Epidemiological studies have linked obesity with basal-like breast cancer risk in both premenopausal and postmenopausal women, suggesting that obesity may affect tumor phenotype by skewing the microenvironment toward support of more aggressive tumor cell phenotypes (typically ER/PR−, HER-2−). In order to study the interactions between adipocytes and breast tumor cells, we developed an adipocyte-breast cancer cell co-culture system. The adipocytes SGBS (Simpson-Golabi-Behmel Syndrome) and human ER positive breast cancer MCF7 cells were co-cultured for 24 hours. Following co-culture, the cells from the inserts (MCF7 cells) and wells (SGBS cells) were collected separately and total RNA was then isolated. The mRNA levels of the NADPH oxidase subunit NOX4 and hypoxia-inducible factor 1α (HIF1α) were determined by RT-PCR and normalized to 18S RNA levels. When SGBS cells were co-cultured with MCF7 cells, SGBS NOX4 and HIF1α mRNA levels increased more than 10 fold and 3 fold, respectively, while ...
LY317615 (Enzastaurin) IC50 in tumors possess different metastasis potential. Although microfluidic technology provides better control over co-culture microenvironment, many systems insert hundreds or hundreds of cells in the gadget still, therefore they absence one cell quality as typical co-culture in petri meals.6C14 Although the solo cell co-culture on-chip allows for isolating solo cells in the step, there are even now two critical problems to be resolved: 1) Thanks to the small amount of secreted protein from solo cell, constant perfusion can easily wash apart the secretion and impair cell-cell interaction thus; and 2) As the system goals to research the heterogeneity of one cells, the chamber-chamber cross-talk, which can trigger unwanted connections, should end up being removed. In the prior functions reported on the one cell-cell connections15,16, the co-culture microenvironment of each cell group was not isolated completely. Hence, the cross-talk among different co-culture environments ...
LY317615 (Enzastaurin) IC50 in tumors possess different metastasis potential. Although microfluidic technology provides better control over co-culture microenvironment, many systems insert hundreds or hundreds of cells in the gadget still, therefore they absence one cell quality as typical co-culture in petri meals.6C14 Although the solo cell co-culture on-chip allows for isolating solo cells in the step, there are even now two critical problems to be resolved: 1) Thanks to the small amount of secreted protein from solo cell, constant perfusion can easily wash apart the secretion and impair cell-cell interaction thus; and 2) As the system goals to research the heterogeneity of one cells, the chamber-chamber cross-talk, which can trigger unwanted connections, should end up being removed. In the prior functions reported on the one cell-cell connections15,16, the co-culture microenvironment of each cell group was not isolated completely. Hence, the cross-talk among different co-culture environments ...
Video articles in JoVE about retinoblastoma protein include In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells, Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software, Analysis of Cell Cycle Position in Mammalian Cells, Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models, Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea, In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration, An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus, A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis, Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets, Assessing Replication and Beta Cell Function in Adenovirally-transduced
Methods Human MSCs were transduced with a lentiviral vector containing TRAIL IRES-GFP under the control of a tetracycline dependent promoter. Successful transduction was measured using flow cytometry and immunoblotting. The biological activity of MSCTRAIL was determined using co-culture experiments. 5×105 MPM cells were stained withDiI and plated with 5×105 MSCTRAIL cells. After 24 h doxycycline (10 μg/ml) was added to induce TRAIL production and left for 48 h. Both cells and supernatant were collected and stained with Annexin V and DAPI to detect apoptosis and death respectively onflow cytometry. ...
Applying biological molecules from cell membranes to the surfaces of artificial materials is opening peepholes on the very basics of cell-to-cell interaction.
Glioblastoma (GBM) is a tumor of the central nervous system. After surgical removal and standard therapy, recurrence of tumors is observed within 6-9 months because of the high migratory behavior and the infiltrative growth of cells. Here, we investigated whether carnosine (β-alanine-l-histidine), which has an inhibitory effect on glioblastoma proliferation, may on the opposite promote invasion as proposed by the so-called
Bacterial mating or conjugation is the transfer of DNA from one bacterium to another via direct cell-to-cell contact through a mating pore. My current research uses the genetically-tractable bacterium Bacillus subtilis as a model system to explore the function and subcellular localization of a putative component of the bacterial mating pore apparatus. I have been characterizing the protein ConE (formerly YddE) which is encoded on the B. subtilis conjugal element ICEBs1. ConE is related to proteins encoded on conjugal elements in numerous bacteria, including the Gram-positive pathogens S. aureus, C. difficile, and L. monocytogenes. ConE belongs to a large superfamily of ATP-dependent pumps involved in the extrusion of proteins and DNA through membrane pores. I have shown that ConE and its ATPase domain are essential for mating of ICEBs1. In addition, ConE-GFP localizes at the cell poles, in close association with the membrane (see Figure). Given ConEs localization, ATPase domain, and ...
Bacterial mating or conjugation is the transfer of DNA from one bacterium to another via direct cell-to-cell contact through a mating pore. My current research uses the genetically-tractable bacterium Bacillus subtilis as a model system to explore the function and subcellular localization of a putative component of the bacterial mating pore apparatus. I have been characterizing the protein ConE (formerly YddE) which is encoded on the B. subtilis conjugal element ICEBs1. ConE is related to proteins encoded on conjugal elements in numerous bacteria, including the Gram-positive pathogens S. aureus, C. difficile, and L. monocytogenes. ConE belongs to a large superfamily of ATP-dependent pumps involved in the extrusion of proteins and DNA through membrane pores. I have shown that ConE and its ATPase domain are essential for mating of ICEBs1. In addition, ConE-GFP localizes at the cell poles, in close association with the membrane (see Figure). Given ConEs localization, ATPase domain, and ...
In the present work, we report evidence that in addition to its role on the proliferation of pancreatic progenitor cells, the mesenchyme is crucial in controlling the timing of pancreatic β-cell differentiation. When we cultured rat embryonic pancreatic epithelium in the absence of its surrounding mesenchyme, we found that Ngn3 expression was turned on rapidly and then turned off a few days later, in keeping with the reported in vivo pattern (15,25). Our filter-separation experiments indicate that the mesenchyme-induced delay in Ngn3 induction requires direct contact between the epithelium and the mesenchyme. This result fits in with recent data indicating that direct cell-cell contact between epithelial and mesenchymal cells suppresses β-cell formation (26). We also found that Ngn3 expression occurred far earlier without mesenchyme, within a few hours compared with 3 days with mesenchyme either in vitro or in vivo. This acceleration in Ngn3 induction resembles the pancreatic phenotype of mice ...
The present invention relates to a multicellular, three-dimensional, living mammalian tissue. The tissue is produced by a co-culture process wherein two distinct types of mammalian cells are co-cultured in a rotating bioreactor which is completely filled with culture media and cell attachment substrates. As the size of the tissue assemblies formed on the attachment substrates changes, the rotation of the bioreactor is adjusted accordingly.
The differential expression analysis revealed that the expression of 34 genes were significantly up-regulated in CAFs compared with those in NFs. Furthermore, the pathway analysis showed that cytoskeletal signaling pathway was up-regulated and TGFb1 played crucial roles as one of upstream regulators in CAFs. Real-time imaging showed that the motility of CAFs was significantly greater on extracellular matrix. Intriguingly, the motility of CAFs conferred invasiveness on GC cells co-cultured with CAFs. ...
HYBRID REFRIGERATED CENTRIFUGE All in one unit from micro tube rotors to multi-place swing rotors. A reliable trump card for centrifuge... read more ...
When I wrote about the NRDCs new Stewardship Index for Specialty Crops, and asked whether industrial monoculture was the real path to sustainable farming, the response from many of our readers was unsurprisingly
The macrophage colony-stimulating factor-deficient bone marrow stromal cell line OP9, derived from osteopetrotic mice, is known to support hematopoietic stem cell (HSC) expansion as well as hematopoietic differentiation of embryonic stem cells. Coculture of HSC in the OP9 system requires cytokine support to achieve significant cell expansion. Recently, we reported extensive expansion without cell senescence of cord blood (CB)-derived HSC cocultured with OP9 stromal cells for more than 18 weeks with a single cytokine support using human thrombopoietin (TPO). In this study, we evaluated the efficiency of the OP9/TPO coculture system to sustain long-term hematopoiesis of adult, granulocyte colony-stimulating factor mobilized human peripheral blood (PB) CD34(+) cells. Maximum cell expansion was attained during the first 4 weeks of coculture. At the same time, the maximum progenitor cell expansion was demonstrated by the production of colony-forming cells and cobblestone area-forming cells. In contrast to
Obesity is associated with a state of chronic, low-grade inflammation. Recent studies have demonstrated that obese adipose tissue is characterized by increased infiltration of macrophages, suggesting that they are an important source of inflammation in the adipose tissue.13-15,24 It is, therefore, important to elucidate the signals that attract macrophages to the adipose tissue and to dissect the molecular mechanisms whereby adipocytes and macrophages communicate within the adipose tissue. Here we developed an in vitro coculture system composed of differentiated 3T3-L1 adipocytes and the macrophage cell line RAW264. The data herein suggest that our coculture system provides the unique in vitro experimental model system to investigate the functional interaction between adipocytes and macrophages within the adipose tissue.. This study demonstrates for the first time that the coculture of 3T3-L1 and RAW264 results in marked upregulation of proinflammatory cytokines, such as MCP-1, IL-6, and TNF-α, ...
The skeleton is the most common site of prostate cancer bone metastasis, and at present, there are no curable treatments for these patients. To further understand what stimulates tumor cell growth in the bone microenvironment and to find suitable therapies, reliable model systems are needed. For this purpose, we have developed an in vitro co-culture system that can be used to study interactions between tumor cells and murine calvarial bones. To validate the model, we measured the release of collagen fragments and monitored changes in expression levels of genes normally expressed during active bone remodeling.. One of the major reasons why prostate cancer cells colonize bone is the abundance of tumor-stimulating factors, such as insulin-like growth factors (IGFs), present in this milieu. We found that the IGF-1 receptor (IGF-1R) was one of the most highly activated receptor tyrosine kinases in tumor cell lines stimulated with bone conditioned media. Since IGF-1 is known to be a strong survival ...
Objective: Peroxisome proliferator-activated receptors (PPARs) are therapeutic targets for fibrates and thiazolidinediones, which are commonly used to ameliorate hyperlipidemia and hyperglycemia in type 2 diabetes mellitus (T2DM). In this study, we evaluated whether activation of PPARα and PPARγ stimulates neoangiogenesis.. Research design and Methods: We used selective synthetic PPARα and PPARγ agonists and investigated their angiogenic potentials in vitro and in vivo.. Results: Activation of PPARα and PPARγ leads to endothelial tube formation in an endothelial/interstitial cell co-culture assay. This effect is associated with increased production of the angiogenic cytokine Vascular Endothelial Growth Factor (VEGF). Neovascularization also occurs in vivo, when PPARα and PPARγ agonists are used in the murine corneal angiogenic model. No vascular growth is detectable when PPARα and PPARγ agonists are respectively used in PPARα knock-out mice and mice treated with a specific PPARγ ...
Breast cancer is a major public health issue for women worldwide and 70% of the disease preferentially metastasizes to bone resulting in its destruction. Advanced breast cancer releases osteoclastogenic factors to promote bone resorption. As bone metastasis with breast cancer is associated with high mortality, osteoclastogenesis is a potential therapeutic target. We have shown previously that benzyl isothiocyanate (BITC), which is present in edible cruciferous vegetables, inhibits breast cancer development in a transgenic mouse model. The present study was designed to determine effect of BITC on breast cancer-induced osteoclastogenesis. We employed well-established in vitro osteoclast co-culture system of Raw 264.7 murine macrophage cells with human breast cancer cells for this assessment. BITC treatment significantly inhibited the number as well as size of osteoclasts in a dose dependent manner in every co-culture experiment. In addition, BITC downregulated both mRNA and protein levels of ...
The ligament-bone interface is a complex structure that comprises ligament, fibrocartilage, and bone. We hypothesize that mesenchymal stem cells cocultured in between ligament and bone cells, on a hybrid silk scaffold with sections suitable for each cell type, would differentiate into fibrocartilage. The section of scaffold for osteoblast seeding was coated with hydroxyapatite. A trilineage coculture system (osteoblasts-BMSCs-fibroblasts) on a hybrid silk scaffold was established. RT-PCR results and immunohistochemistry results demonstrated that BMSCs cocultured between fibroblasts and osteoblasts had differentiated into the fibrocartilaginous lineage. The morphological change was also observed by SEM observation. A gradual transition from the uncalcified to the calcified region was formed in the cocultured BMSCs from the region that directly interacted with fibroblasts to the region that directly interacted with osteoblasts. The role of transforming growth factor β3 (TGF-β3) in this ...
Through multiple stimulation and co-culture experiments, these authors were able to discover aspects pertaining to MDSC proliferation and function. They found that IL-6 induced MDSC expansion via STAT3 signaling, and that this pathway is active during HIV infection. Furthermore, they elucidated the suppressive role of MDSC by showing that these subset of cells can suppress IFN-gamma production by producing reactive nitrogen and oxygen species to hinder the T cell response and also induce T cell IL-10 secretion. Furthermore, they found that MDSCs, in addition to suppressing immunity directly, also suppress immunity indirectly by stimulating Treg proliferation. Lastly, to show that these in vitro studies were relevant to human HIV infection, they sampled the blood of patients with untreated HIV infection and found an increased population of MDSCs (as well as higher plasma IL-6) compared to HIV-uninfected individuals. Thus, these authors not only have elucidated the role of MDSCs in immune ...
CD28 is one of the key molecules for co-stimulatory signalling in T cells. Here, novel ligands (affibodies) showing selective binding to human CD28 (hCD28) have been selected by phage display technology from a protein library constructed through combinatorial mutagenesis of a 58-residue three-helix bundle domain derived from staphylococcal protein A. Analysis of selected affibodies showed a marked sequence homology and biosensor analyses showed that all investigated affibodies bound to hCD28 with micromolar affinities (K-D). No cross-reactivity towards the related protein human CTLA-4 could be observed. This lack of cross-reactivity to hCTLA-4 suggests that the recognition site on hCD28 for the affibodies resides outside the conserved MYPPPYY motif. The apparent binding affinity for hCD28 could be improved through fusion to an Fc fragment fusion partner, resulting in a divalent presentation of the affibody ligand. For the majority of selected anti-CD28 affibodies, in co-culture experiments ...
Background: Increasing the complexity of in vitro systems to mimic three-dimensional tissues and the cellular interactions within them will increase the reliability of data that were previously collected with in vitro systems. In vivo vascularization is based on complex and clearly defined cell-matrix and cell-cell interactions, where the extracellular matrix (ECM) seems to play a very important role. The aim of this study was to monitor and visualize the subcellular and molecular interactions between endothelial cells (ECs), fibroblasts, and their surrounding microenvironment during vascular morphogenesis in a three-dimensional coculture model. Methods: Quantitative and qualitative analyses during the generation of a coculture tissue construct consisting of endothelial cells and fibroblasts were done using transmission electron microscopy and immunohistochemistry. Results: Dynamic interactions were found in cocultures between ECs, between fibroblasts (FBs), between ECs and FBs, and between the cells
Amyotrophic lateral sclerosis (ALS) is the most common motor neuron disease. ALS is believed to be a non-cell autonomous condition, as other cell types, including astrocytes, have been implicated in disease pathogenesis. Hence, to facilitate the development of therapeutics against ALS, it is crucial to bette
Many pathologies are characterized by poor blood vessel growth and reduced nutrient delivery to the surrounding tissue, introducing a need for tissue engineered blood vessels. Our lab has developed a 3D co-culture method to grow interconnected networks of pericyte-invested capillaries, which can anastamose with host vasculature following implantation to restore blood flow to ischemic tissues. However, if the engineered vessels contain endothelial cells (ECs) that are misaligned or contain wide junctional gaps, they may function improperly and behave more like the pathologic vessels that nourish tumors. The purpose of this study was to test the resistance to permeability of these networks in vitro, grown with different stromal cell types, as a metric of vessel functionality. A fluorescent dextran tracer was used to visualize transport across the endothelium and the pixel intensity was quantified using a customized MATLAB algorithm. In fibroblast-EC co-cultures, the dextran tracer easily penetrated
Fast-Track proposals will not be accepted. Direct-to-Phase II proposals will not be accepted. Number of anticipated awards: 1-3 Budget (total costs, per award): Phase I: $225,000 for 9 months; Phase II: $1,500,000 for 2 years PROPOSALS THAT EXCEED THE BUDGET OR PROJECT DURATION LISTED ABOVE MAY NOT BE FUNDED. Phase II information is provided only for informational purposes to assist Phase I offerors with their long-term strategic planning. Summary There has been an increased focus in the life sciences industry on the use of more complex 3D cellular and tissue models to help provide physiologically relevant platforms to be used in in-vitro drug testing. Nearly all high throughput in-vitro drug testing experiments utilize multi-well microtiter plates to act as a vessel where individual reactions between the biological model and the sample under test occur. The use of these more complex 3D cellular models has driven the use of more complex microtiter plates, namely cell culture insert-plates (CCIP) ...
The control of the activities of regulatory B (Breg) cells in immune disorders is an emerging therapeutic strategy for the recovery of immune homeostasis. Manipulating B cells using numerous drugs in vivo affect their regulatory functions, although a direct link has not yet been demonstrated. Glatiramer acetate (GA) is a synthetic polypeptide that is used in the treatment of inflammatory and autoimmune diseases. We experimented on an in vitro coculture system to determine its direct effects on the Breg cell properties of human B cells. We found that GA improves the B cell-dependent control of T cells immune responses. When B cells are stimulated by GA, the T cell proliferation and their Th1 IFN-γ production are further inhibited, whereas the B cell production of IL-10 is further enhanced. GA binds preferentially to the memory B cells and the activation of sorted B cell subsets shows that GA-dependent increased Breg cell activities are specifically supported by the B cells memory compartment. ...
Bone marrow stromal cells protect hematopoietic cells and provide drug resistance by delivering bunch of variable proteins. Thus, alterations of protein expression are typically associated with cell-cell signal transduction and regulation of cellular functions. Co-culture models of bone marrow stromal cells and hematopoietic cells are often used in studies of their crosstalk. Studies of altered protein expression initiated by stromal cell/hematopoietic cell interactions are an important new trend in microenvironmental research. There has been no report to date of global quantitative proteomics analysis of crosstalk between hematopoietic cells and stromal cells. In this study, we analyzed quantitative proteomes in a co-culture system of stromal HS5 cells and hematopoietic KG1a cells, and simultaneously tracked differentially expressed proteins in two types of cells before and after co-culture by stable isotope labeling by amino acids in cell culture (SILAC) method. We have shown that in co-cultured KG1a,
Purpose: : Preliminary studies have identified Klk13 as a putative autoantigen during the immunopathogenesis of experimental autoimmune lacrimal keratoconjunctivitis (ALKC). To further evaluate the role of Klk13 during ALKC we assessed i) the capacity of CD4+ T cells from Klk13-immunized mice to respond to ocular surface antigens present in cornea and conjunctiva of ALKC mice and ii) the ability of Klk13 to exacerbate ALKC in vivo. Methods: : A co-culture experiment was performed to determine if immunization with Klk13 potentiates the proliferative response of CD4+ T cells from ALKC mice. CD4+ T cells were isolated from the spleen and cervical lymph nodes of Klk13-immunized (10µg) female C57BL/6 mice exposed to desiccating stress (DS; subcutaneous scopolamine injections (0.5 mg/0.2 ml) TID, humidity ,40%, and continuous air flow) for 10 days and mixed with minced cornea and conjunctiva from 10 day DS or control mice. Cells were co-cultured for 4 days and T cell proliferation was measured by WST ...
TY - JOUR. T1 - Cd18/ICAM-1-dependent oxidafive NF-κB activation leading to nitric oxide production in rat Kupffer cells cocultured with syngeneic hepatoma cells. AU - Kurose, Iwao. AU - Saito, Hidetsugu. AU - Miura, Soichiro. AU - Ebinuma, Hirotoshi. AU - Higuchi, Hajime. AU - Watanabe, Naoyuki. AU - Zeki, Shigeyuki. AU - Nakamura, Tetsuya. AU - Takaishi, Masaaki. AU - Ishii, Hiromasa. PY - 1997/3/1. Y1 - 1997/3/1. N2 - Previous studies have indicated that nitric oxide (NO) released from Kupffer cells modulates biological viability of cocultured hepatoma cells. This study was designed to evaluate the mechanisms by which Kupffer cells synthesize and release NO in response to cocultured hepatoma cells. Kupffer cells isolated from male Wistar rats were cocultured with rat hepatoma cell line, AH70 cells. The sum of nitrite and nitrate levels increased in the culture medium of Kupffer cells with AH70 cells as compared with those of Kupffer cells or AH70 cells alone. Increased expressions of iNOS ...
With the adoption of 3D methods and complex co-culturing becoming a growing trend, cell culture media optimization has also become a challenging factor for researchers. Lonza helps researchers ease this transition with BulletKit™ Growth Media, which are robust in supporting co-culture studies. Depending on the specific needs of the co-culture, Lonzas scientific support team can offer proven solutions supported by a long history of published literature. For cancer research in particular, where both cell lines and primary cells are equally significant, BulletKit™ Media provides researchers with added flexibility and reduced variability across their experiments as the same media can be used to support the growth of both cell types. This also simplifies experimental design. Take for instance, MEGM™ Mammary Growth BulletKit™, which has been utilized by researchers to support growth of Lonzas primary mammary epithelial cells and well-established breast cell lines, MCF-10A and MCF-12. For ...
Link to Pubmed [PMID] - 28275106. Open Biol 2017 03;7(3). The disrupted-in-schizophrenia 1 () gene was identified as a genetic risk factor for chronic mental illnesses (CMI) such as schizophrenia, bipolar disorder and severe recurrent depression. Insoluble aggregated DISC1 variants were found in the cingular cortex of sporadic, i.e. non-genetic, CMI patients. This suggests protein pathology as a novel, additional pathogenic mechanism, further corroborated in a recent transgenic rat model presenting DISC1 aggregates. Since the potential role of aggregation of DISC1 in sporadic CMI is unknown, we investigated whether DISC1 undergoes aggregation in cell culture and could spread between neuronal cells in a prion-like manner, as shown for amyloid proteins in neurodegenerative diseases. Co-culture experiments between donor cells forming DISC1 aggregates and acceptor cells showed that 4.5% of acceptor cells contained donor-derived DISC1 aggregates, thus indicating an efficient transfer DISC1 aggregates ...
DESCRIPTION (provided by applicant): With few exceptions, stem cell injections into the heart produce short-term improvement in cardiac function but a notable lack of integration and differentiation. This temporal improvement in cardiac function may be due to paracrine factors, but the process is not well understood. Recently, mitochondria have been observed to be transferred through tunneling nanotubes (TNTs) into myocytes, which might explain the transient effects of stem cell injections on improved cellular function. An understanding of mitochondrial transfer between stem cells and myocytes might enable rescue of failing cardiomyocytes by directed transfer of functional mitochondrial replacements. Significantly, there are pediatric genetic mitochondrial cardiomyopathies that in which such transfer could be particularly useful as a therapy. TNT formation has been observed in many settings; however, difficulty in capturing such a small structure using currently available tissue-sectioning ...
After verifying that we could in fact degrade catechol into 2-hydroxymuconate semialdehyde using our xylE construct (BBa_J33204), we wondered if we could take this any further. What if we could convert this by-product page into hydrocarbons too? As catechol is the breakdown product of a number of different degradation pathways in bacteria, this could be particularly useful.. As 2-hydroxymuconate semialdehyde can be further metabolized to pyruvate and acetaldehyde (Harayama S et al., 1987), it seemed possible that these products could be routed into the fatty acid biosynthesis pathway and converted to alkanes using the PetroBrick or the OleT enzyme. Given that the Catechol 2,3-dioxygenase reaction is extracellular, it creates a possible scenario in which cells with the xylE construct could be co-cultured with Petrobrick-containing cells to cooperatively metabolise catechol into hydrocarbons. In order to test this, we followed this protocol, where we co-cultured cells expressing our xylE construct ...
0041] Different combinations of cells can also be co-cultured in order to model metabolic disease such as diabetes (e.g., Diabetes Type II (DTII) or Diabetes Type I (DTI)). In some cases, pancreatic beta cells differentiated from iPS from a healthy human subject are co-cultured with adipocytes derived from one or more of the following human subjects: (1) healthy non-obese human subjects; (2) obese with DTII human subjects; (3) obese without DTII human subjects; (4) non-obese with DTII human subjects; (5) non-obese human subjects at risk for DTII or obesity; (6) non-obese human subjects at risk for DTII; (7) DTII human subjects at risk for obesity; (8) obese with DTI human subjects; (9) obese without DTI human subjects; (10) non-obese with DTI human subjects; (11) non-obese human subjects at risk for DTI or obesity; (12) non-obese human subjects at risk for DTI; and/or (13) DTI human subjects at risk for obesity. Pancreatic beta cells differentiated from iPS from a healthy human subject can also ...
The mechanism for cancer cell death was elucidated via several experiments. ESR spectroscopy, which measures free radicals, showed that hKDCs produce reactive oxygen species (ROS). Decreased anti-tumor activity in the presence of FeTPPS, a free radical inhibitor, confirmed this result. When cancer cells and hKDCs were separated by a semi-permeable membrane, decreased anti-tumor behavior was observed suggesting that hKDCs kill cancer cells via direct cell-cell contact. Additionally, a staining experiment that allowed visualization of the dying cells as well as the death of certain apoptosis-resistant cancer cells suggests that the cells die by necrosis (catastrophic cell death) rather than apoptosis (programmed cell death). This, too, is promising because cancer cells are particularly adept at avoiding apoptosis, so a treatment that includes a necrotic mechanism might be effective against these cells. ...
Blood-brain barrier (BBB) function is regulated by dynamic interactions among cell types within the neurovascular unit, including astrocytes and endothelial cells. Co-culture models of the BBB typically involve astrocytes seeded on two-dimensional (2D) surfaces, which recent studies indicate cause astrocytes to express a phenotype similar to that of reactive astrocytes in situ. We hypothesized that the culture conditions of astrocytes would differentially affect their ability to modulate BBB function in vitro. Brain endothelial cells were grown alone or in co-culture with astrocytes.
We have initiated studies to identify membrane polypeptides of radial glial cells that contribute to the selective cell-cell recognition and migration events in developing brain. Of several polyclonal antisera evaluated, one (D4), developed against formaldehyde fixed type 1 cerebellar glial cells, immunolabels the free surface of cortical and cerebellar astroglial and radial glial cells in a patchy pattern. In dissociated glial-neuronal cell cocultures, microdomains of immunoreactivity are detected at the site where the somal region of cells with the morphology of migrating neurons is in contact with an elongated glial cell fiber. Microdomains are absent from oligodendrocytes, process-bearing astrocytes, and neurons. The surface microdomains do not colocalize with components that compose focal adhesion plaques--integrin subunits, vinculin, or actin--and their integrity appears to require an intact microtubule rather than actin cytoskeleton. Furthermore, microdomain structure is maintained in the ...
in BMC Cell Biology (2006), 7. Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel ... [more ▼]. Background: The absorptive and goblet cells are the main cellular types encountered in the intestine epithelium. The cell lineage Caco-2 is a model commonly used to reproduce the features of the bowel epithelium. However, there is a strong debate regarding the value of Caco-2 cell culture to mimick in vivo situation. Indeed, some authors report in Caco-2 a low paracellular permeability and an ease of access of highly diffusible small molecules to the microvilli, due to an almost complete lack of mucus. The HT29-5M21 intestinal cell lineage is a mucin-secreting cellular population. A co-culture system carried out in a serum-free medium and comprising both Caco-2 and HT29-5M21 cells was developed. The systematic use of a co-culture system requires the ...
Specter, S C.; Bendinelli, M; Ceglowski, W S.; and Friedman, H, "Macrophage-induced reversal of immunosuppression by leukemia viruses." (1978). Subject Strain Bibliography 1978. 66 ...
Dr. Lee has spearheaded in the development of in vitro tissue models and novel bioreactors in the field of cardiovascular tissue engineering in the past several years. She has developed spontaneously beating heart chambers exhibiting key characteristics of native heart for the first time, which is truly novel and powerful for answering questions that cannot easily be approached in vivo. She has also developed a uniaxial and a biaxial stretching device, which can be used to study the impact of mechanical stimulation on engineered cardiac tissues. More importantly, she has developed a novel flow bioreactor, which allows culture of microvasculature in vitro under the influence of flow, which is critical for any functional tissues. To the best of her knowledge, this was the first gel-based flow bioreactor, which provide a new basis for subsequent co-culture studies with various cell types to develop complex engineered tissue constructs with vascularization capacity, which is extremely critical for ...
Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson. Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. npj Microgravity, 2017; 3 (1) DOI: 10.1038/s41526-017-0011-2 ...
Jennifer Barrila, Jiseon Yang, Aurélie Crabbé, Shameema F. Sarker, Yulong Liu, C. Mark Ott, Mayra A. Nelman-Gonzalez, Simon J. Clemett, Seth D. Nydam, Rebecca J. Forsyth, Richard R. Davis, Brian E. Crucian, Heather Quiriarte, Kenneth L. Roland, Karen Brenneman, Clarence Sams, Christine Loscher, Cheryl A. Nickerson. Three-dimensional organotypic co-culture model of intestinal epithelial cells and macrophages to study Salmonella enterica colonization patterns. npj Microgravity, 2017; 3 (1) DOI: 10.1038/s41526-017-0011-2 ...
Purpose: Ovarian cancer (OCa) is the deadliest of all gynecologic cancers, mainly because the disease, in most cases, has progressed to advanced stages when it is diagnosed. Our published studies have established a role for 1alpha,25-dihydroxyvitamin D3 (1,25D3) and its receptors (VDR) in suppressing ovarian tumor growth. The purpose of the present studies is to investigate the effect of the hormone and its receptors on OCa invasion.. Approaches: Our microarray analyses have identified a group of cytokines of which the expression was suppressed by 1,25D3. Molecular analyses were used to define the mechanisms underlying the cytokine suppression. Trans-well assays were used to measure the in vitro effect of 1,25D3 on OCa invasion and migration. Ex vivo omental organ co-culturing with luciferase-marked human OCa cells was developed to investigate the effect of 1,25D3 and VDR on the ability of OCa cells to invade omentum. Syngeneic ovarian tumor models in VDR null mice were established to test the ...
BioAssay record AID 432521 submitted by ChEMBL: Activation of mouse DN32 cells in co-cultured with CD1d-transfected RAW cells assessed as IL-2 release after 16 hrs by ELISA.
The co-culture of primary CLL cells with BMSC, CD40L and CpG ODN promotes an immunophenotype comparable to that from proliferating CLL cells found in PB(A) PBMC
传统观念认为癌症的进展仅仅是由癌细胞基因和表型变化的多个过程所致。但最近20年的研究显示肿瘤微环境(tumor microenvironment,TME)对于肿瘤行为的影响是同等重要的。TME的组成包括局部的基质细胞,如定植的成纤维细胞(cancer-associated fibroblasts,CAF)和巨噬细胞,远处招募的细胞如内皮细胞、免疫细胞包括髓系和淋巴系细胞、骨髓来源的前体细胞和循环中的血小板。TME能够分泌影响并调控肿瘤表型的分子,如能揭示成瘤细胞与微环境之间的关系,必定能够为肿瘤的发生发展及治疗等一系列难题提供全新的视角。
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Light rays enter the eye through the cornea. The cornea refracts the light rays in such a way that they pass freely through the pupil, the opening in the centre of the iris.. After passing through the pupil, the light rays pass through the lens. Using what flexibility it has, the lens focuses the light rays on the retina. The image is upside down, because it has passed through the cornea which is curved.. This upside-down image is sent along the optic nerve to the brain, which interprets the image and flips it the right way up.. If our corneas are not perfectly round (astigmatism) or if they are more curved or more flat relative to the size of our eyeball (short or long sightedness), the image does not focus directly on the retina but either in front or behind it. This gives us blurred vision which can be corrected by wearing glasses or contact lenses, or by having laser eye surgery or lens surgery. ...
The generation of immune responses involves the coordinated communication between cells through direct cell-to-cell contact and the release of various soluble factors binding to their respective receptors. Of the many soluble factors and receptors known, there is growing evidence that the release of extracellular adenosine triphosphate (ATP) and its subsequent activation of cell-surface ligand-gated cation channels belonging to the family of P2X receptors (P2X1-7) play important roles in communication between immune cells [1]. Much of what is understood about the roles of extracellular ATP and the activation of P2X receptors during an immune response has been inferred from in vitro studies requiring the addition of exogenous ATP or from studies using rodent models of inflammation and immunity [2]. Nevertheless our understanding of the extracellular ATP-P2X receptor axes operating between immune cells, including CD4+ T cells, during immune responses is limited.
Polyclonal B cell response is a natural mode of immune response exhibited by the adaptive immune system of mammals. It ensures that a single antigen is recognized and attacked through its overlapping parts, called epitopes, by multiple clones of B cell. In the course of normal immune response, parts of pathogens (e.g. bacteria) are recognized by the immune system as foreign (non-self), and eliminated or effectively neutralized to reduce their potential damage. Such a recognizable substance is called an antigen. The immune system may respond in multiple ways to an antigen; a key feature of this response is the production of antibodies by B cells (or B lymphocytes) involving an arm of the immune system known as humoral immunity. The antibodies are soluble and do not require direct cell-to-cell contact between the pathogen and the B-cell to function. Antigens can be large and complex substances, and any single antibody can only bind to a small, specific area on the antigen. Consequently, an ...
Poster Presentations; section 3 - Gene Transfer and Regulation, section 4 - Marker Assisted Selection, Section 5 - Transgenics, Section 6 - Biotic Stresses; Proceedings of the 9th International Wheat Genetics Symposium, Saskatoon, Saskatchewan, Canada 2-7 August ...
Light micrograph (LM) showing conjugation tube and zygote in Spirogyra sp. green algae. Conjugation is a form of sexual reproduction in which micronuclei are exchanged by direct cell-to-cell contact or by a bridge-like connection between two cells. Fast Green stain. Magnification 400x at 35mm. - Stock Image C009/3792
Communication among cells via direct cell-cell contact by connexin gap junctions, or between cell and extracellular environment via pannexin channels or connexin hemichannels, is a key factor in cell...
Our laboratory is interested in the wound response and repair of epithelium, neurons and extracellular matrix. 1. In the healthy cornea there are no blood vessels and thus the tissue provides an excellent model to study the communication between nerves and epithelium following injury. Primary neurons are co-cultured with epithelial cells to ask how signaling pathways communicate. These include but are not limited to glutamatergic and purinergic receptor-mediated calcium transients. 2. In addition to these signaling events we have found that when epithelial cells are injured nucleotides are released immediately and cause a distinct phosphorylation of EGF receptor residues that ultimately affect the ability of a cell to migrate. 3. To study controlling factors on extracellular matrix or stromal formation we have developed scaffold-free long-term cultures and these have recently enabled us to study the role of hypoxia, which occurs in a diurnal pattern in this tissue as one sleeps. Our experiments ...
Read "Direct cell-cell communications and social behavior of cells in mammals, protists, and bacteria. Possible causes of multicellularity, Russian Journal of Developmental Biology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Collective cell migration is often considered in the context of just one population of cells on the move, but can often actually involve several cell types, raising obvious questions about how such mass movement is coordinated. By studying the interaction between neural crest (NC) cells and placodal cells in developing Xenopus laevis and zebrafish embryos, Eric Theveneau et al. have identified a chase-and-run behaviour that ensures successful neural crest migration and placode segregation, and gained insight into the underlying molecular mechanisms.. The playground antics of placode cells and NC cells could be seen both in vivo and in vitro. By labelling both cell types in embryos, the researchers saw that placode cells moved randomly until NC cells arrived on the scene, at which point they ran away, leaving gaps in the placode region. In co-culture studies, placodal cells were seen to encourage NC cells - by secreting the chemoattractant Sdf1 - to chase them, but once the NC cells got ...
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon. ...
Estern blot analysisAGS cells were co-cultured with H. pylori strain 60190 or its isogenic mutants at an MOI of 100:1 for 2, 4, or 8 hours. Protein lysates were
Abstract The microenvironment of established tumours is often immunosuppressed, and this allows tumours to grow and disseminate without being eliminated by the patients immune sys..
Co-cultivation of production cells at the limit of the in vitro age is an assay we offer to test for latent retroviruses for our clients. Essentially, your production cells are plated along with susceptible cell lines that exhibit stronger phenotypic CPE when the virus enters them. Thus, viruses that are expressed as non-infectious particles or exhibit low level of expression in production cells are detected ...
Microvision is een fabrikant die gespecialiseerd is in vitreoretinale chirurgie. Trocar sets, messen, backflush handstukken, 27 gauge canules, laserfibers. Microvision maakt het in eigen beheer. Bekijk de gehele catalogus of neem contact met ons op om uw wensen te bespreken.. ...
Introduction Different molecular processes lead to metastatic spread and the occurrence of tumour cell resistance to therapeutic interventions. Among them, the epithelial to mesenchymal transition (EMT) process plays a key role. During EMT, epithelial tumour cells lose the expression of specific proteins and adopt the phenotype of mesenchymal cells. These structural conversions are substantially dependent on the tumour microenvironment. EMT of tumour cells can induce drug resistance and metastasis. Thus, EMT inhibition may offer a new strategy for overcoming tumour progression.. Methods To evaluate EMT in a non-small cell lung cancer (NSCLC) model a 2D and a 3D- cell culture system was applied using both the human lung cancer cell line (A549) and the human lung fibroblast cell line (SV-80). For generating 3D cell spheroids, a novel system was established consisting of 96-well hanging drop microtiter plates (InSphero AG, Zürich, Switzerland). 2D co-culture assays were performed in transwell ...
Direct cell-to-cell contact regulates a variety of cellular functions, including cell activation and cytokine production.42 43 45 We attempted to determine whether the interaction between monocytes and endothelial cells induces MCP-1 production from these cell types, because this interaction constantly occurs in recruitment of monocytes and because production of MCP-1 would affect subsequent monocyte transendothelial migration. Our results indicate that migrated monocytes express MCP-1 protein during the process of transmigration. Cocultures of resting monocytes and unstimulated or IL-1-prestimulated endothelial cell monolayers induced an increase in the amounts of soluble MCP-1 secreted into the medium. MCP-1 secreted in supernatants exhibited chemotactic and transendothelial activities for monocytes, and its biological effect was confirmed by the disappearance of the activity in the presence of anti-MCP-1 Ab. Quantification of mRNA levels indicates that augmentation was mediated at the level ...
Human embryonic stem cells (hESCs) can proliferate extensively in culture and give rise to progeny of the three germ layers. Several reports suggested that mouse and hESCs may attenuate immune responses. In this study, we focused on the mechanism by which hESCs inhibit T cell responses. Using coculture experiments, we demonstrate that hESCs inhibit cytokine secretion and T cell proliferation in response to potent T cell activators. Furthermore, we show that hESCs downmodulate the TCR-associated CD3-ζ chain. These effects are maintained when hESCs are replaced by their conditioned media and can be restored by the addition of l-arginine to hESC-conditioned media or by treatment of hESCs with a specific arginase inhibitor. Moreover, we show arginase-I expression and activity in hESCs. We further demonstrate that mouse ESCs (mESCs) similarly inhibit T cell activation via arginase I, suggesting an evolutionary conserved mechanism of T cell suppression by ESCs. In addition, we demonstrate that ...
Vascular smooth muscle cells [VSMC], mononuclear cells [MNC] and their local interaction are known to play key roles in atherosclerotic plaque development and destabilization. To study the cellular interactions an in-vitro co-culture model was established. Peripheral blood MNC were isolated by density gradient centrifugation and marked with fluorescence-labeled acetylated low-density lipoprotein [Fl-ac-LDL]. Mitomycin-treated non-proliferating VSMC were seeded semi-confluently and co-cultured with the labeled MNC in a ratio of 1:3. Immuno-cytochemistry revealed that, within 4 weeks of co-culture, ,20% of the VSMC cells had acquired Fl-ac-LDL. Transdifferentiation processes of the MNC into VSMC had not taken place but could be excluded by xenogenic cell composure (rat VSMC and human MNC) and subsequent quantitative RT-PCR with human specific primers for VSMC markers. Separation of the cells in a trans-well co-culture system resulted in complete absence of double-labeled cells, too. Moreover, ...
The effect of different electron acceptors on substrate degradation was studied in pure and mixed cultures of various hydrogenotrophic homoacetogenic, methanogenic, sulfate-reducing, fumarate-reducing and nitrate-ammonifying bacteria. Two different species of these bacteria which during organic substrate degradation produce and consume hydrogen, were cocultured on a substrate which was utilized only by one of them. Hydrogen, which was excreted as intermediate by the first strain (and reoxidized in pure culture), could, depending on the hydrogen acceptor present, also be used by the second organism, resulting in interspecies hydrogen transfer. The efficiency of H2 transfer was similar when methanol, lactate or fructose were used as organic substrate, although the free energy changes of fermentative H2 formation of these substrates are considerably different. In coculture experiments nitrate or fumarate,sulfate, CO2/CH4,sulfur or CO2/acetate were the preferred electron acceptors, and an increasing ...
Exposure of animals to perfluorooctanoic acid (PFOA), a surfactant used in emulsion polymerization processes causes early pregnancy loss, delayed growth and development of fetuses. The mechanisms of action are largely unknown. We studied the effect of PFOA on implantation using an in vitro spheroid-endometrial cell co-culture model. PFOA (10-100. μM) significantly reduced Jeg-3 spheroid attachment on RL95-2 endometrial cells. PFOA also suppressed β-catenin expression in Jeg-3 cells. The Wnt agonist Wnt3a stimulated β-catenin expression in Jeg-3 cells and reversed the PFOA suppression of the spheroid attachment. The putative PFOA receptors (PPARα, β, γ) present in both cell lines were not affected by PFOA (0.01-100. μM). The PPARα antagonist MK886 restored the β-catenin and E-cadherin expression levels in Jeg-3 cells and reversed the suppression of the spheroid attachment caused by PFOA. Taken together, PFOA suppresses spheroid attachment through PPARα and Wnt signaling pathways via down
Mesenchymal stem cells (MSCs) transplanted at sites of nerve injury are thought to promote functional recovery by producing trophic factors that induce survival and regeneration of host neurons. To evaluate this phenomenon further, we quantified in human MSCs neurotrophin expression levels and their effects on neuronal cell survival and neuritogenesis. Screening a human MSC cDNA library revealed expressed transcripts encoding BDNF and beta-NGF but not NT-3 and NT-4. Immunostaining demonstrated that BDNF and beta-NGF proteins were restricted to specific MSC subpopulations, which was confirmed by ELISA analysis of 56 separate subclones. Using a co-culture assay, we also demonstrated that BDNF expression levels correlated with the ability of MSC populations or subclones to induce survival and neurite outgrowth in the SH-SY5Y neuroblastoma cell line. However, these MSC-induced effects were only partially inhibited by a neutralizing anti-BDNF antibody. MSCs were also shown to promote neurite ...
TY - JOUR. T1 - Regulation of immunoglobulin production in pokeweed mitogen-stimulated cultures of lymphocytes from young and old adults. AU - Ceuppens, J. L.. AU - Goodwin, James. PY - 1982. Y1 - 1982. N2 - Polyclonal (pokeweed mitogen-stimulated) humoral immune responses were studied in cultures of peripheral blood mononuclear cells (PBMC) or T + B cell combinations of young (20 to 40 yr) and aged (,65 yr) individuals. Immunoglobulin (Ig) production was measured in the culture supernatants with an ELISA method. Cultures of lymphocytes from old and young individuals produced similar amounts of IgG and IgM. In co-culture experiments of old or young B cells with old or young T cells, the old T cells consistently provided more help for old and young B cells than the young T cells did. In addition, isolated OKT4(+) cells of old people provided more help than OKT4(+) cells of young people, whereas the suppressor activity of isolated OKT8(+) cells from old people was diminished. Because young or old ...

WikiGenes - Coculture TechniquesWikiGenes - Coculture Techniques

Psychiatry related information on Coculture Techniques. *The von Ebners-like protein mRNA was induced after 4 h of co-culture ... Chemical compound and disease context of Coculture Techniques. *The coculture of cells expressing the HIV-1 envelope ... Gene context of Coculture Techniques. *Additionally, in cocultures in which spleen cells were derived from receptor-deficient ... Disease relevance of Coculture Techniques. *Antiestrogen-induced TGF-beta from MCF-7 cells inhibits the growth of an estrogen ...
more infohttp://www.wikigenes.org/e/mesh/e/23239.html

Calcium sensitivity for hypoxia in PGNs with PC-12 cells in co-culture.Calcium sensitivity for hypoxia in PGNs with PC-12 cells in co-culture.

Coculture Techniques. Ganglia, Sensory / cytology*. Intracellular Space / drug effects, metabolism. Neurons / cytology, drug ... Calcium sensitivity of petrosal ganglion neurons (PGNs) to chemical stimuli with and without PC-12 cells in co-culture instead ...
more infohttp://www.biomedsearch.com/nih/Calcium-Sensitivity-Hypoxia-in-PGNs/19536473.html

Spontaneous immortalisation of ensheathing cells from adult rat olfactory nerve.Spontaneous immortalisation of ensheathing cells from adult rat olfactory nerve.

Coculture Techniques. Female. Male. Nerve Regeneration. Neurites / physiology. Olfactory Nerve / cytology*, immunology, ... Rolf B1.T cells support the regrowth of neurites from adult retinal ganglion cells in vitro in a heterologous co-culture system ...
more infohttp://www.biomedsearch.com/nih/Spontaneous-immortalisation-ensheathing-cells-from/8833195.html

CCL3 | Cancer Genetics WebCCL3 | Cancer Genetics Web

Co-culture experiments that exposed healthy donor PBMCs to lung cancer cells in vitro were performed to evaluate the effect on ... Further mechanism dissection showed that co-culturing T cells with RCC cells could produce more IGF-1 and FGF-7, which may ... Mechanism dissection suggested that co-culturing T cells with RCC cells released more T cell attraction factors, including IFN- ... induced similar changes in cytokine gene and protein expression by healthy donor PBMCs in an in vitro co-culture system. We ...
more infohttp://www.cancerindex.org/geneweb/CCL3.htm

The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and...The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and...

The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and ... The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and ...
more infohttps://www.promocell.com/resources/the-liquid-overlay-technique-is-the-key-to-formation-of-co-culture-spheroids-consisting-of-primary-osteoblasts-fibroblasts-and-endothelial-cells/

T Cells Cooperate With Palmitic Acid in Induction of Beta Cell Apoptosis - PubMedT Cells Cooperate With Palmitic Acid in Induction of Beta Cell Apoptosis - PubMed

Coculture Techniques Actions. * Search in PubMed * Search in MeSH * Add to Search ...
more infohttps://pubmed.ncbi.nlm.nih.gov/19463182/

Platelet-derived growth factor-AB limits the extent of myocardial infarction in a rat model: feasibility of restoring impaired...Platelet-derived growth factor-AB limits the extent of myocardial infarction in a rat model: feasibility of restoring impaired...

Coculture Techniques. *Coronary Vessels (drug effects) *Disease Models, Animal. *Endothelium, Vascular (cytology, drug effects ...
more infohttp://www.curehunter.com/public/pubmed11827927.do

Role of alpha(v)beta(3) integrin in osteoclast migration and formation of the sealing zone. | CureHunterRole of alpha(v)beta(3) integrin in osteoclast migration and formation of the sealing zone. | CureHunter

Coculture Techniques. *Dose-Response Relationship, Drug. *Fluorescent Antibody Technique. *Mice. *Mice, Inbred BALB C ... using primary rat osteoclasts and murine pre-fusion osteoclast-like cells formed in a co-culture system. We show that: (1) ...
more infohttp://www.curehunter.com/public/pubmed10547359.do

Knockdown of myosin II in OL-DRG cocultures enhances my | Open-iKnockdown of myosin II in OL-DRG cocultures enhances my | Open-i

A) 3-wk-old myelinating OL-DRG cocultures stained for MBP, Olig2, and neurofilament. Coc ... Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. ( ... Coculture Techniques. *Cytoskeleton/metabolism. *Ganglia, Spinal/cytology. *Heterocyclic Compounds with 4 or More Rings/ ... fig5: Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. (A) 3-wk-old myelinating OL-DRG cocultures stained ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC2542477_jcb1821171f05&req=4

Effect of Spalax, rat and mouse fibroblasts on Spalax-d | Open-iEffect of Spalax, rat and mouse fibroblasts on Spalax-d | Open-i

Coculture Techniques. *Culture Media, Conditioned/pharmacology. *DNA Fragmentation/drug effects. *Fibroblasts/drug effects/ ...
more infohttps://openi.nlm.nih.gov/detailedresult.php?img=PMC3750378_1741-7007-11-91-11&req=4

Search | IOVS | ARVO JournalsSearch | IOVS | ARVO Journals

TAGS: cytokine, chemokines, coculture techniques, t-lymphocyte, retinal pigment cell Invest. Ophthalmol. Vis. Sci.. 2012; 53(13 ... TAGS: inflammation, chemokines, cell lines, chemotaxis, coculture techniques, monocytes, t-lymphocyte, proteins, intraocular ... Chemokine Expression in Retinal Pigment Epithelial ARPE-19 Cells in Response to Coculture with Activated T Cells PDF ... Retinal pigment epithelial cells co-cultured with activated T cells upregulate chemoattractants but do not increase monocyte ...
more infohttps://iovs.arvojournals.org/solr/searchresults.aspx?author=Helene+B.+Juel

Magiran | Iranian Journal of Medical Sciences، Volume:43 Issue:2, 2018Magiran | Iranian Journal of Medical Sciences، Volume:43 Issue:2, 2018

Keywords: Bone, Bones, Coculture Techniques, Osteoblasts, Osteoclasts, Bone Remodeling Abstract View Paper Brief Report ... Secondly, OBs and OCs were co-cultured at the ratios of 1 OC: 1 OB, 1 OC: 4 OBs, 2 OCs: 1 OB, and 1 OC: 2 OBs. Tartrate- ... Optimization of the Static Human Osteoblast/Osteoclast Co-culture System James Jam Jolly, Kok-Yong Chin, Mohd Fozi Nur Farhana ... Therefore, the present study was carried out to optimize an OB/OC co-culture system utilizing the human OB cell line hFOB 1.19 ...
more infohttps://www.magiran.com/volume/128544

Platelets induce monocyte differentiation in serum-free coculture  - University of Regensburg Publication ServerPlatelets induce monocyte differentiation in serum-free coculture - University of Regensburg Publication Server

Coculture Techniques. MESH. Culture Media, Serum-Free. MESH. Enzyme-Linked Immunosorbent Assay. MESH. ... To characterize some of the cell-cell connections that may be important for MO differentiation we cocultured human MO with ... To characterize some of the cell-cell connections that may be important for MO differentiation we cocultured human MO with ... Platelets induce monocyte differentiation in serum-free coculture. Journal of leukocyte biology 63 (4), pp. 469-76. ...
more infohttps://epub.uni-regensburg.de/14321/

Prostaglandin E2 Regulates Th17 Cell Differentiation and Function Through Cyclic AMP and EP2/EP4 Receptor Signaling - PubMedProstaglandin E2 Regulates Th17 Cell Differentiation and Function Through Cyclic AMP and EP2/EP4 Receptor Signaling - PubMed

Coculture Techniques Actions. * Search in PubMed * Search in MeSH * Add to Search ... PGE2 is required for IL-23-IL-1β-induced IL-17 production in a monocyte-naive T cell co-culture system. (A) Real-time PCR ... C) Human naive CD4+ T cells were co-cultured with monocytes (1:1), activated with anti-CD3 antibody, and cultured for 11 d in ...
more infohttps://pubmed.ncbi.nlm.nih.gov/19273625/

kat: A high-efficiency retroviral transduction system for primary human T lymphocytes<...kat: A high-efficiency retroviral transduction system for primary human T lymphocytes<...

TY - JOUR. T1 - kat. T2 - A high-efficiency retroviral transduction system for primary human T lymphocytes. AU - Finer, Mitchell H.. AU - Dull, Thomas J.. AU - Qin, Lu. AU - Parson, Deborah. AU - Roberts, Margo R.. PY - 1994/1/1. Y1 - 1994/1/1. N2 - We describe a novel retroviral packaging system in which high titer amphotropic retrovirus was produced without the need to generate stable producer clones. kat expression vectors, which produce high levels of retroviral vector transcripts and retroviral packaging functions, were transfected into 293 cells followed by virus harvest 48 hours posttransfection. Viral titers as high as 3.8 proviral copies/cell/mL of frozen supernatant in 3T3 cells were obtained, 10- to 50-fold greater than transient viral titers reported using 3T3-based retroviral packaging lines. Cocultivation of primary human CD8+ T lymphocytes after transient transfection of 293 cells with kat plasmids resulted in transduction efficiencies of 10% to 40%, 5- to 10-fold greater compared ...
more infohttps://jhu.pure.elsevier.com/en/publications/kat-a-high-efficiency-retroviral-transduction-system-for-primary--3

Suppression of the immunologic response to peanut during immunotherapy is often transient<...Suppression of the immunologic response to peanut during immunotherapy is often transient<...

Peanut- and dust mite-induced expression of TH2 cytokines was reduced in DC-T-cell cocultures during immunotherapy. This was ... Peanut- and dust mite-induced expression of TH2 cytokines was reduced in DC-T-cell cocultures during immunotherapy. This was ... Peanut- and dust mite-induced expression of TH2 cytokines was reduced in DC-T-cell cocultures during immunotherapy. This was ... Peanut- and dust mite-induced expression of TH2 cytokines was reduced in DC-T-cell cocultures during immunotherapy. This was ...
more infohttps://jhu.pure.elsevier.com/en/publications/suppression-of-the-immunologic-response-to-peanut-during-immunoth-4

Department of Radiology - Research Output
     - Penn StateDepartment of Radiology - Research Output - Penn State

Advanced MR Imaging Techniques for Evaluation of the Heart and Great Vessels. Boxerman, J. L., Mosher, T. J., McVeigh, E. R., ... Smooth muscle cell proliferation in response to co-culture with venous and arterial endothelial cells. Waybill, P. N., ...
more infohttps://pennstate.pure.elsevier.com/en/organisations/department-of-radiology-2/publications/?type=%2Fdk%2Fatira%2Fpure%2Fresearchoutput%2Fresearchoutputtypes%2Fcontributiontojournal%2Farticle&ordering=publicationYearThenTitle&descending=false&page=4

Changes in immunoregulatory lymphocyte populations in patients with histoplasmosis | SpringerLinkChanges in immunoregulatory lymphocyte populations in patients with histoplasmosis | SpringerLink

Functional activity determined by coculture techniques correlated closely with T-lymphocyte subset measurements. These distinct ...
more infohttps://link.springer.com/article/10.1007%2FBF00915042

Impact of the c-MybE308G mutation on mouse myelopoiesis and dendritic cell development | ScholarBank@NUSImpact of the c-MybE308G mutation on mouse myelopoiesis and dendritic cell development | [email protected]

Coculture Techniques. Dendritic Cells. Female. Flow Cytometry. Hematopoietic Stem Cells. Mice. Mice, Inbred C57BL. Myeloid ... coculture. cytology. dendritic cell. female. genetics. metabolism. point mutation. spleen. stroma cell. CD8 antigen. protein c ... since L-DCs arise in vitro by direct differentiation from HSPCs co-cultured over splenic stroma. The non-lethal c-Myb mutation ...
more infohttps://scholarbank.nus.edu.sg/handle/10635/161197

Inhibitory effect of glycyrrhizin on the neutrophil-dependent increase of R5 HIV replication in cultures of macrophages<...Inhibitory effect of glycyrrhizin on the neutrophil-dependent increase of R5 HIV replication in cultures of macrophages<...

In the presence of GL, these soluble factors were not detected in co-cultures of MDMs and PMNs exposed to R5 HIV. In addition, ... In the presence of GL, these soluble factors were not detected in co-cultures of MDMs and PMNs exposed to R5 HIV. In addition, ... In the presence of GL, these soluble factors were not detected in co-cultures of MDMs and PMNs exposed to R5 HIV. In addition, ... In the presence of GL, these soluble factors were not detected in co-cultures of MDMs and PMNs exposed to R5 HIV. In addition, ...
more infohttps://ucdavis.pure.elsevier.com/en/publications/inhibitory-effect-of-glycyrrhizin-on-the-neutrophil-dependent-inc

Inherited 7S immunoglobulin deficiency in chickens: Presence of suppressor T cells that suppress synthesis of 7S immunoglobulin...Inherited 7S immunoglobulin deficiency in chickens: Presence of suppressor T cells that suppress synthesis of 7S immunoglobulin...

In co-culture, UCD 140 PBL specifically inhibited 7S Ig but not IgM synthesis of PWM-stimulated PBL from normal birds. Normal ... In co-culture, UCD 140 PBL specifically inhibited 7S Ig but not IgM synthesis of PWM-stimulated PBL from normal birds. Normal ... In co-culture, UCD 140 PBL specifically inhibited 7S Ig but not IgM synthesis of PWM-stimulated PBL from normal birds. Normal ... In co-culture, UCD 140 PBL specifically inhibited 7S Ig but not IgM synthesis of PWM-stimulated PBL from normal birds. Normal ...
more infohttps://ucdavis.pure.elsevier.com/en/publications/inherited-7s-immunoglobulin-deficiency-in-chickens-presence-of-su

ZFIN Publication: Lin et al., 2010ZFIN Publication: Lin et al., 2010

Coculture Techniques. *Embryo, Nonmammalian/anatomy & histology. *Embryo, Nonmammalian/physiology. *Frizzled Receptors/genetics ... Using co-culture assays to generate directional Wnt5b cues, we demonstrate that Ryk-expressing cells migrate away from the ...
more infohttp://zfin.org/ZDB-PUB-100730-13
  • Immunologic outcomes included measurement of spontaneous and stimulated basophil activity by using automated fluorometry (histamine) and flow cytometry (activation markers and IL-4), measurement of allergen-induced cytokine expression in dendritic cell (DC)-T-cell cocultures by using multiplexing technology, and measurement of MHC II and costimulatory molecule expression on DCs by using flow cytometry. (elsevier.com)
  • citation needed] In 1982, Cohen joined Bourn Hall Clinic as an Embryologist, working with Patrick Steptoe and Robert G. Edwards on techniques geared towards improving human conception through in vitro fertilization (IVF). (wikipedia.org)
  • To characterize some of the cell-cell connections that may be important for MO differentiation we cocultured human MO with lymphocytes and/or with platelets. (uni-regensburg.de)
  • Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. (nih.gov)
  • To further investigate the contribution of OL myosin II on myelin formation, we infected OPC-DRG cocultures with shRNA lentivirus against the regulatory chain of MLC. (nih.gov)
  • Knockdown of MLC in OPC-DRG cocultures resulted in a significant increase in myelination similar to that observed in cultures treated with blebbistatin. (nih.gov)
  • In addition to being noninvasive and relatively pain free, Coculture can be performed during a short office visit. (wikipedia.org)
  • Incubation of cocultures with lentivirus resulted in the preferential infection of OPC rather than neurons, as indicated by GFP expression (Fig. S3, available at http://www.jcb.org/cgi/content/full/jcb.200802091/DC1). (nih.gov)
  • Peanut- and dust mite-induced expression of T H 2 cytokines was reduced in DC-T-cell cocultures during immunotherapy. (elsevier.com)