A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Parasitic plants that form a bushy growth on branches of host trees which are in the order Santalales. It includes the Christmas mistletoe family (VISCACEAE), the showy mistletoe family (LORANTHACEAE) and the catkin mistletoe family (Eremolepidaceae). The composition of toxins, lectins, tyramine, phenethylamines, and other compounds may be affected by the host.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
Transient complete or partial monocular blindness due to retinal ischemia. This may be caused by emboli from the CAROTID ARTERY (usually in association with CAROTID STENOSIS) and other locations that enter the central RETINAL ARTERY. (From Adams et al., Principles of Neurology, 6th ed, p245)
Cellular signaling in which a factor secreted by a cell affects other cells in the local environment. This term is often used to denote the action of INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS on surrounding cells.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Designations for persons whose names are not known or who wish to remain anonymous (anonyms) and for persons who wish to conceal or obscure their identity by assuming a fictitious name (pseudonyms).
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Benzoic acid or benzoic acid esters substituted with one or more bromine atoms.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Established cell cultures that have the potential to propagate indefinitely.
Technique for treating DEHYDRATION and WATER-ELECTROLYTE IMBALANCE by subcutaneous infusion of REHYDRATION SOLUTIONS.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Receptor protein-tyrosine kinases involved in the signaling of GLIAL CELL-LINE DERIVED NEUROTROPHIC FACTOR ligands. They contain an extracellular cadherin domain and form a receptor complexes with GDNF RECEPTORS. Mutations in ret protein are responsible for HIRSCHSPRUNG DISEASE and MULTIPLE ENDOCRINE NEOPLASIA TYPE 2.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Transmission of emotions, ideas, and attitudes between individuals in ways other than the spoken language.
The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.
A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A pair of highly specialized muscular canals extending from the UTERUS to its corresponding OVARY. They provide the means for OVUM collection, and the site for the final maturation of gametes and FERTILIZATION. The fallopian tube consists of an interstitium, an isthmus, an ampulla, an infundibulum, and fimbriae. Its wall consists of three histologic layers: serous, muscular, and an internal mucosal layer lined with both ciliated and secretory cells.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Adherence of cells to surfaces or to other cells.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Progenitor cells from which all blood cells derive.
A cell line derived from cultured tumor cells.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
An enzyme that hydrolyzes 1,6-alpha-glucosidic branch linkages in glycogen, amylopectin, and their beta-limit dextrins. It is distinguished from pullulanase (EC 3.2.1.41) by its inability to attack pullulan and by the feeble action of alpha-limit dextrins. It is distinguished from amylopectin 6-glucanohydrolase (EC 3.2.1.69) by its action on glycogen. With EC 3.2.1.69, it produces the activity called "debranching enzyme". EC 3.2.1.68.
Analogs or derivatives of scopolamine.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A family of anaerobic, coccoid to rod-shaped METHANOBACTERIALES. Cell membranes are composed mainly of polyisoprenoid hydrocarbons ether-linked to glycerol. Its organisms are found in anaerobic habitats throughout nature.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.
Penetrating and non-penetrating injuries to the spinal cord resulting from traumatic external forces (e.g., WOUNDS, GUNSHOT; WHIPLASH INJURIES; etc.).
A cylindrical column of tissue that lies within the vertebral canal. It is composed of WHITE MATTER and GRAY MATTER.
A surgical specialty concerned with the treatment of diseases and disorders of the brain, spinal cord, and peripheral and sympathetic nervous system.
Renewal or physiological repair of damaged nerve tissue.
A subspecialty of internal medicine concerned with morphology, physiology, and pathology of the blood and blood-forming tissues.
The long large endothelium-lined venous channel on the top outer surface of the brain. It receives blood from a vein in the nasal cavity, runs backwards, and gradually increases in size as blood drains from veins of the brain and the DURA MATER. Near the lower back of the CRANIUM, the superior sagittal sinus deviates to one side (usually the right) and continues on as one of the TRANSVERSE SINUSES.

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (1/7795)

Interleukin-12 (IL-12) is a potent antitumor cytokine, which induces and enhances the activity of natural killer (NK) cells, lymphokine activated killer (LAK) cells and cytotoxic T lymphocytes (CTL). IL-12 also stimulates IFN-gamma production from both T cells and NK cells. In this study, we transfected methylcholanthrene-induced fibrosarcoma (MCA-D) with TNF gene and investigated the therapeutic effect of TNF gene-transduced cancer vaccine and whether the vaccination effect is enhanced by systemic administration of recombinant IL-12 (rIL-12), in a murine model. TNF gene-transduced cancer vaccine or systemic administration of rIL-12 showed slight or moderate inhibition of pre-established tumor. However, simultaneous application of the vaccine and rIL-12 resulted in complete eradication. The cytotoxicity of CTL against parental tumor cells was enhanced with the combination of the vaccine and rIL-12, and IFN-gamma production from spleen cells also increased synergistically. Our findings show that synergistic enhancement of CTL activity and IFN-gamma production could play an important role in the antitumor effect of combination therapy using TNF gene-transduced cancer vaccine and rIL-12.  (+info)

Regulation of neurotrophin-3 expression by epithelial-mesenchymal interactions: the role of Wnt factors. (2/7795)

Neurotrophins regulate survival, axonal growth, and target innervation of sensory and other neurons. Neurotrophin-3 (NT-3) is expressed specifically in cells adjacent to extending axons of dorsal root ganglia neurons, and its absence results in loss of most of these neurons before their axons reach their targets. However, axons are not required for NT-3 expression in limbs; instead, local signals from ectoderm induce NT-3 expression in adjacent mesenchyme. Wnt factors expressed in limb ectoderm induce NT-3 in the underlying mesenchyme. Thus, epithelial-mesenchymal interactions mediated by Wnt factors control NT-3 expression and may regulate axonal growth and guidance.  (+info)

Reciprocal control of T helper cell and dendritic cell differentiation. (3/7795)

It is not known whether subsets of dendritic cells provide different cytokine microenvironments that determine the differentiation of either type-1 T helper (TH1) or TH2 cells. Human monocyte (pDC1)-derived dendritic cells (DC1) were found to induce TH1 differentiation, whereas dendritic cells (DC2) derived from CD4+CD3-CD11c- plasmacytoid cells (pDC2) induced TH2 differentiation by use of a mechanism unaffected by interleukin-4 (IL-4) or IL-12. The TH2 cytokine IL-4 enhanced DC1 maturation and killed pDC2, an effect potentiated by IL-10 but blocked by CD40 ligand and interferon-gamma. Thus, a negative feedback loop from the mature T helper cells may selectively inhibit prolonged TH1 or TH2 responses by regulating survival of the appropriate dendritic cell subset.  (+info)

Endothelial cells modulate the proliferation of mural cell precursors via platelet-derived growth factor-BB and heterotypic cell contact. (4/7795)

Embryological data suggest that endothelial cells (ECs) direct the recruitment and differentiation of mural cell precursors. We have developed in vitro coculture systems to model some of these events and have shown that ECs direct the migration of undifferentiated mesenchymal cells (10T1/2 cells) and induce their differentiation toward a smooth muscle cell/pericyte lineage. The present study was undertaken to investigate cell proliferation in these cocultures. ECs and 10T1/2 cells were cocultured in an underagarose assay in the absence of contact. There was a 2-fold increase in bromodeoxyuridine labeling of 10T1/2 cells in response to ECs, which was completely inhibited by the inclusion of neutralizing antiserum against platelet-derived growth factor (PDGF)-B. Antisera against PDGF-A, basic fibroblast growth factor, or transforming growth factor (TGF)-beta had no effect on EC-stimulated 10T1/2 cell proliferation. EC proliferation was not influenced by coculture with 10T1/2 cells in the absence of contact. The cells were then cocultured so that contact was permitted. Double labeling and fluorescence-activated cell sorter analysis revealed that ECs and 10T1/2 cells were growth-inhibited by 43% and 47%, respectively. Conditioned media from contacting EC-10T1/2 cell cocultures inhibited the growth of both cell types by 61% and 48%, respectively. Although we have previously shown a role for TGF-beta in coculture-induced mural cell differentiation, growth inhibition resulting from contacting cocultures or conditioned media was not suppressed by the presence of neutralizing antiserum against TGF-beta. Furthermore, the decreased proliferation of 10T1/2 cells in the direct cocultures could not be attributed to downregulation of the PDGF-B in ECs or the PDGF receptor-beta in the 10T1/2 cells. Our data suggest that modulation of proliferation occurs during EC recruitment of mesenchymal cells and that heterotypic cell-cell contact and soluble factors play a role in growth control during vessel assembly.  (+info)

Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis. (5/7795)

OBJECTIVE: To investigate the features of synovial stromal cells established from patients with rheumatoid arthritis (RA), and to define these cells as nurse cells. METHODS: Synovial nurse-like stromal cell lines (RA-SNCs) were established from patients with RA. These cell lines were examined for morphology, pseudoemperipolesis activity, cell surface markers, and cytokine production. The interaction between these RA-SNCs and a synovial tissue B cell clone was also examined. RESULTS: RA-SNCs had nurse cell activity. They spontaneously produced interleukin-6 (IL-6), IL-8, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. Furthermore, they produced IL-1beta and tumor necrosis factor alpha and expressed higher levels of the other cytokines after coculture with the B cell clone. Proliferation and Ig production by the B cell clone were dependent on direct contact with RA-SNCs. CONCLUSION: These results indicate that the RA-SNCs were nurse cells. The findings suggest that RA-SNCs may play an important role in the pathogenesis of RA by producing large amounts of cytokines and maintaining infiltrating lymphocytes.  (+info)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (6/7795)

Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease.  (+info)

In vitro hematopoietic and endothelial cell development from cells expressing TEK receptor in murine aorta-gonad-mesonephros region. (7/7795)

Recent studies have shown that long-term repopulating hematopoietic stem cells (HSCs) first appear in the aorta-gonad-mesonephros (AGM) region. Our immunohistochemistry study showed that TEK+ cells existed in the AGM region. Approximately 5% of AGM cells were TEK+, and most of these were CD34(+) and c-Kit+. We then established a coculture system of AGM cells using a stromal cell line, OP9, which is deficient in macrophage colony-stimulating factor (M-CSF). With this system, we showed that AGM cells at 10.5 days postcoitum (dpc) differentiated and proliferated into both hematopoietic and endothelial cells. Proliferating hematopoietic cells contained a significant number of colony-forming cells in culture (CFU-C) and in spleen (CFU-S). Among primary AGM cells at 10.5 dpc, sorted TEK+ AGM cells generated hematopoietic cells and platelet endothelial cell adhesion molecule (PECAM)-1(+) endothelial cells on the OP9 stromal layer, while TEK- cells did not. When a ligand for TEK, angiopoietin-1, was added to the single-cell culture of AGM, endothelial cell growth was detected in the wells where hematopoietic colonies grew. Although the incidence was still low (1/135), we showed that single TEK+ cells generated hematopoietic cells and endothelial cells simultaneously, using a single-cell deposition system. This in vitro coculture system shows that the TEK+ fraction of primary AGM cells is a candidate for hemangioblasts, which can differentiate into both hematopoietic cells and endothelial cells.  (+info)

Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8(+) T cells resulting in a failure to lyse tumor cells. (8/7795)

Dendritic cells (DC) are critically involved in the initiation of primary immune processes, including tumor rejection. In our study, we investigated the effect of interleukin-10 (IL-10)-treated human DC on the properties of CD8(+) T cells that are known to be essential for the destruction of tumor cells. We show that IL-10-pretreatment of DC not only reduces their allostimulatory capacity, but also induces a state of alloantigen-specific anergy in both primed and naive (CD45RA+) CD8(+) T cells. To investigate the influence of IL-10-treated DC on melanoma-associated antigen-specific T cells, we generated a tyrosinase-specific CD8(+) T-cell line by several rounds of stimulation with the specific antigen. After coculture with IL-10-treated DC, restimulation of the T-cell line with untreated, antigen-pulsed DC demonstrated peptide-specific anergy in the tyrosinase-specific T cells. Addition of IL-2 to the anergic T cells reversed the state of both alloantigen- or peptide-specific anergy. In contrast to optimally stimulated CD8(+) T cells, anergic tyrosinase-specific CD8(+) T cells, after coculture with peptide-pulsed IL-10-treated DC, failed to lyse an HLA-A2-positive and tyrosinase-expressing melanoma cell line. Thus, our data demonstrate that IL-10-treated DC induce an antigen-specific anergy in cytotoxic CD8(+) T cells, a process that might be a mechanism of tumors to inhibit immune surveillance by converting DC into tolerogenic antigen-presenting cells.  (+info)

cell-cell contact in transwell systems - posted in Tissue and Cell Culture: I would like to conduct transwell experiments with two cell types and wants to investigate the effect of cell-cell interaction between these 2 cell types. Do anyone have protocol of doing co-culture of these cells in the transwell system (ie. cell type 1 on the upper surface of the insert; while cell type 2 on the lower surface of the insert). Thank you very much.
Muscle regeneration involves the coordination of myogenesis and revascularization to restore proper muscle function. Myogenesis is driven by resident stem cells termed satellite cells (SC), whereas angiogenesis arises from endothelial cells and perivascular cells of preexisting vascular segments and the collateral vasculature. Communication between myogenic and angiogenic cells seems plausible, especially given the number of growth factors produced by SC. To characterize these interactions, we developed an in vitro coculture model composed of rat skeletal muscle SC and microvascular fragments (MVF). In this system, isolated epididymal MVF suspended in collagen gel are cultured over a rat SC monolayer culture. In the presence of SC, MVF exhibit greater indices of angiogenesis than MVF cultured alone. A positive dose-dependent effect of SC conditioned medium (CM) on MVF growth was observed, suggesting that SC secrete soluble-acting growth factor(s). Next, we specifically blocked VEGF action in SC ...
Results ICAM-1 expression was significantly up-regulated upon the introduction of TNF-α under all conditions in HUVEC (figure 1). However, baseline expression was increased when co-cultured with both NHDF (2.0 vs 1.3, p,0.001) and HUASMC (6.5 vs 1.3, p,0.001). This meant that ICAM-1 expression at 12 hours was also significantly higher in co-culture with NHDF (8.3 vs 5.2, p,0.001) and HUASMC (11.0 vs 5.2, p,0.001). Moreover, there was a moderate relationship between HUVEC ICAM-1 expression and the cell ratio when in co-culture with NHDF, where decreasing NHDF resulted in decreased ICAM-1 in HUVEC (R2=0.45).. TNF-α caused an increase in ICAM-1 expression in NHDF under monoculture conditions (Fig. 2); this up-regulation was significantly reduced in co-culture conditions with HUVEC (1.7 vs 5.1, p,0.001). A similar trend was observed when in co-culture with HUASMC (2.7 vs 5.1, p,0.001), except the baseline expression of ICAM-1 was also increased (2.8 vs 1.1, p,0.001).. Constitutive production of ...
TY - JOUR. T1 - Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction. AU - Che, Zhong Min. AU - Jung, Tae Heob. AU - Choi, Jung Hee. AU - Yoon, Do Jun. AU - Jeong, Hyun Jeong. AU - Lee, Eun Ju. AU - Kim, Jin. PY - 2006/7/21. Y1 - 2006/7/21. N2 - The roles of tumor stroma in carcinogenesis are still unclear. This study was aimed at designing an in vitro model for investigating the effects of stromal fibroblasts in the invasive growth of squamous cell carcinoma. Using two cancer cell lines, we performed three-dimensional co-culture with dermal equivalents to evaluate the effects of fibroblasts in cancer invasion. In vitro models for cellular interaction study were designed as follows: a collagen gel-based direct co-culture model (C-Dr) and a collagen gel-based indirect co-culture model (C-In). The invasive growth was found only in the dermal equivalents with fibroblasts. MMP-2 activity could be induced by direct contact between cancer cells and stromal fibroblasts. ...
Novel mechanisms, and molecular mediators, of the pro-tumorigenic effects of cancer-associated fibroblasts (CAFs) have been identified. These include CXCL12/SDF-1-mediated recruitment of bone marrow-derived endothelial precursor cell and pro-metastatic effects of CCL5. Co-culture experiments also su …
Formulations comprising combinations of APCs and tumor cells and APCs and virally infected cells are disclosed. These formulations generally comprise hybridoma of at least one antigen presenting cell
Various mechanisms have been proposed for the development of PNI in cancer cells. Recent studies have proposed the concept of neuron-cancer cell crosstalk; nerve cells have been shown to infiltrate the tumor microenvironment and actively stimulate cancer cell growth by releasing neurotransmitters. In turn, the secretion of neurotrophic growth factors by cancer cells drives the outgrowth of neurons in solid tumors (10). This reciprocal interaction between neurons and cancer cells provides new insights into identifying the novel molecular mechanisms involved, and finally leads to the development of anti-neurogenic therapies. In this regard, the in vitro cancer cell-neuron co-culture models utilized in the present study can provide a proper simulation of the cancer-neuron crosstalk, which evaluates the neurotropism of cancer cells by placing each cell type separately and observing the abilities of cancer cells to migrate and invade in a time-dependent manner. This co-culture model has been widely ...
Cell culture inserts were seeded with 1x105 cells in 250 uL of medium with 0. 1% FBS. Un coated inserts had been utilised for migration assays whereas inserts
Trans-Lux Corp., a tmaker of digital LED and LCD display screens, cites Trumps election victory and his tough talk on manufacturing as the main reason it is bringing production back to the U.S.
Endothelial vascular smooth muscle cell coculture assay for high throughput screening assays to identify antiangiogenic and other therapeutic molecules George A TruskeyDepartment of Biomedical Engineering, Duke University, Durham, NC, USAAbstract: Drug development for many diseases would be aided greatly by accurate in vitro model systems that replicate key elements of in vivo physiology. The recent development of coculture systems of endothelial cells and vascular smooth muscle cells can be extended to high-throughput systems for the identification of compounds for angiogenesis, vascular repair, and hypertension. In this review, the various coculture systems are reviewed, and biologic interactions between endothelial cells and vascular smooth muscle cells are discussed. Key considerations in the design of high-throughput systems are presented, and selected examples are discussed.Keywords: endothelium, vascular smooth muscle cells, angiogenesis, microfluidics
In vitro coculture systems are at the forefront of molecular research due to their increased ability for cell-cell communication. In this study, small airway epithelial cells (SAEC) and human microvascular endothelial cells (HMVEC) were grown separately in monoculture or together in an alveolar-capillary coculture and exposed to either dispersion media control or multi-walled carbon nanotubes (MWC
The recent clinical successes of immune-based cancer therapies highlight the utility of harnessing the cytotoxicity of CD8 + T cells to target tumors. While current immunotherapies are effective at supporting and enhancing the function of tumor-specific T cell responses, these cells remain subject to multiple tolerizing mechanisms in the tumor microenvironment which can ultimately limit their effectiveness. While the function of dedicated regulatory immune cell populations like MDSCs and Tregs are well-studied contributors to tumor tolerance, our studies suggest that additional tumor-mediated mechanisms exist.. Our previous studies indicated that tumor cell lines secreted a soluble factor that was capable of inducing phenotypic and functional changes in normal human T cells that closely resembled those associated with dysfunctional T cells present in the periphery and tumor microenvironment of cancer patients [16, 17]. Because these studies were based on in vitro co-culture experiments, we first ...
The purpose of this study was to characterize an in vitro co-culture model in which fibroblasts grown in a three-dimensional nylon mesh were recombined with human keratinocytes. The cultures were kept for 3 and 5 weeks and then processed for electron microscopy. Keratinocytes showed reconstruction o …
BioTek Notas de Aplicações, 18-Feb-16, High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model
BioTek Notas de Aplicación, 18-Feb-16, High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model
BioTek Notas de Aplicación, 18-Feb-16, High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model
Video articles in JoVE about salmonella typhimurium include Quantification of Cytosolic vs. Vacuolar Salmonella in Primary Macrophages by Differential Permeabilization, Digital PCR-based Competitive Index for High-throughput Analysis of Fitness in Salmonella, Infection of Zebrafish Embryos with Intracellular Bacterial Pathogens, NF-κB-Dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells, Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria, In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration, Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses, Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways, Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers, Coincubation Assay for Quantifying
BACKGROUND. Prostate cancer (PCa) and bone cell interactions are critical in the metastatic phase. Kallikrein 4 (KLK4/hK4) is expressed in both PCa and mineralized tissues. We determined if KLK4/hK4 expression was associated with, and influenced by, the bone environment of metastatic PCa. METHODS. Immunohistochemistry, in vitro co-culture, cell migration, and attachment assays. RESULTS. hK4 was localized to tumor cells and osteoblasts in bone metastases. KLK4/hK4 increased in LNCaP and PC3 cells co-cultured with SaOs2 cells; SaOs2 KLK4/hK4 was unchanged. Co-culture did not affect cell proliferation but altered alkaline phosphatase activity/mRNA levels in SaOs2 cells. KLK4-transfected PC3 cells had increased migration towards SaOs2 conditioned medium and greater attachment to the bone-matrix proteins, collagens I and IV. CONCLUSIONS. hK4 expression and interaction with both tumor cells and osteoblasts suggests a role for hK4 in PCa bone metastasis. Whether this observation is unique to bone ...
Background P. gingivalis, a non-motile, rod-shaped, anaerobic, Gram-negative bacterium is one of the principal sources of periodontal disease. It possesses a number of potential virulence factors thought to be important in the disease process including 5 hemagglutinins (Hag). One of these is HagB. It is a well characterized nonfimbrial adhesin expressed on the surface of P. gingivalis. HagB is very pro-inflammatory and induces robust chemokine and cytokine responses in vitro and in vivo. Since the chemokine and cytokine responses seen from single cells grown in tissue culture often are not representative of the chemokine and cytokine profiles seen in clinical samples or biopsy specimens, we devised a co-culture model of keratinocytes, dendritic cells, and T-cells to test the hypothesis that chemokine and cytokine responses from co-cultured cells would be more representative of responses seen in clinical samples from individuals with periodontal disease than single cell models. Methods and materials HagB
Abstract We proposed an effective strategy for evaluating the targeting specificity of an antibody-conjugated quantum dot (QD) nanoprobe in a coculture system mimicking an in vivo-..
Methods: T cells were activated with anti-CD3/28 antibodies and subsequently transduced with a bicistronic retrovirus encoding tandem Rim-binding domains (FKBP12v36),cloned in-frame with MyD88 and CD40 cytoplasmic signaling molecules, and first generation CAR targeting CD123 (SFG-iMC-CD123.ζ). The effects ofiMC costimulation on CD123-targeted CARs were assessed in coculture assays with CD123+, EGFPluciferase (EGFPluc)-modified leukemic cell lines (KG1, THP-1 and MOLM-13) with and without Rim using the IncuCyte live cell imaging system. IL-2 production was examined by ELISA from coculture supernatants. In vivo efficacy of iMC-CD123.ζ-modified T cells was assessed using an immune-deficient NSG tumor xenograft model. One million EGFPluc-expressing CD123+ THP-1 tumor cells were injected i.v. into the animals, followed by a single i.v. injection on day 7 with 2.5x106 non-transduced or iMC-CD123.ζ-modified T cells. Groups receiving CAR-T cells subsequently received i.p. injections of Rim (1 mg/kg) ...
Our present study has provided, for the first time to our knowledge, a systematic analysis of the inflammation-relevant gene expression in ECs and SMCs in their coculture under static condition and in response to shear stress. We demonstrated that SMCs cause an upregulation of proinflammatory gene expression in ECs located in close proximity and that shear stress acts as a negative regulator for these proinflammatory gene expression in ECs cocultured with SMCs. Under static coculture condition, DNA microarray technology identified 23 inflammation-relevant genes that exhibit significant changes (18 increases and 5 decreases) in their expression in ECs cocultured with SMCs, as compared with the control monocultured ECs. All 18 genes upregulated in ECs by the coculture encode products that serve proinflammatory and thrombogenic functions. These functions include: (1) mediation of leukocyte-EC interaction and signal transduction (ICAM-1, VCAM-1, CD44, and NK-4); (2) promotion of chemotaxis and ...
1. Using sterile forceps, pick up the coverslips containing neurons and place into the bottom of the co-culture plate. Be sure to keep the cells covered with medium. Replace the insert with the microglia coverslip. Alternatively, you could change the medium in the microglial culture completely, and do a wash with fresh medium, before replacing the medium, to get rid of the LPS. Then add the neurons ...
Lastly, we examined heterotypic interactions between tumor cells and cancer associated fibroblasts (CAFs) to understand the mechanism of resistance to lapatinib. Using a 3D co-culture model, we identified significant sub-type-specific changes in gene expression, metabolic, and therapeutic sensitivity profiles of breast cancer cells induced by CAFs. We identified JAK2/STAT3 pathway and CAF-secreted hyaluronan as major factors contributing to CAF-mediated protection. We also found that close spatial proximity to CAFs impacts therapeutic responses by affecting proliferation rates of cancer cells ...
In model 1, target CD4+ T cells (UC blood CD4+ T cells and a T cell line expressing CXCR4 and CD4.403) are cocultured with effector HEK cells with or without Env expression. In model 2, HEK/CD4.403/CXCR4 and HEK/CD4.403 cells are cocultured with effector T cells with or without Env expression. In model 3, target CD4+ T cells are cocultured with HIVNL4-3-infected or uninfected T cells. Expression of gp120 at the surface of HEK.Env, 8.E5, and HIVNL4-3-infected effector cells as well as parental HEK and CEM cells - used as negative controls - were analyzed by flow cytometry after incubation of the cells with PBS (white histograms) or with PBS containing anti-gp120 human polyclonal Ab (black histograms). Bound Ab was detected with a secondary FITC-labeled goat anti-human Ig. Expression analysis of the extracellular domains of CD4 and CXCR4 at the surface of target cells was performed after incubation of the cells with PBS (white histograms) or with anti-CD4 (black histograms) or anti-CXCR4 (gray ...
In the present study, we set out to determine the degrees of residual plasma viremia in a large number of infected individuals receiving ART and to determine immunological or virological parameters that may correlate with residual plasma viremia. Residual plasma viremia was determined in quadruplicate using an automated system to minimize quantitative errors often associated with the detection of extremely low levels of viral RNA. We have demonstrated detectable levels of residual plasma viremia (1-49 copies/mL) in the majority of study participants receiving ART in whom plasma viremia had been suppressed for extended periods of time, as measured by a standard clinical assay (with a limit of detection of 50 copies/mL). Furthermore, we found a correlation between the level of residual plasma viremia and the frequency of CD4+ T cells carrying HIV proviral DNA. Of note, due to the limited amounts of blood obtained from the study subjects, we could not conduct the quantitative coculture assays that ...
Molostvov G, Morris A, Rose P, Basu S, Muller G (February 2004). The effects of selective cytokine inhibitory drugs (CC-10004 and CC-1088) on VEGF and IL-6 expression and apoptosis in myeloma and endothelial cell co-cultures. British Journal of Haematology. 124 (3): 366-75. doi:10.1046/j.1365-2141.2003.04777.x. PMID 14717786 ...
OBJECTIVE: Rheumatoid arthritis (RA) is classically thought of as a Th1, T lymphocyte-driven disease of the adaptive immune system. However, cells of the innate immune system, including neutrophils, are prevalent within the diseased joint, and accumulate in large numbers. This study was undertaken to determine whether cells of the rheumatoid stromal microenvironment could establish an inflammatory environment in which endothelial cells are conditioned in a disease-specific manner to support neutrophil recruitment. METHODS: Human umbilical vein endothelial cells (ECs) and fibroblasts isolated from the synovium or skin of RA patients were established in coculture on opposite sides of porous transwell filters. After 24 hours of EC conditioning, the membranes were incorporated into a parallel-plate, flow-based adhesion assay and levels of neutrophil adhesion to ECs were measured. RESULTS: ECs cocultured with synovial, but not skin, fibroblasts could recruit neutrophils in a manner that was dependent on the
Cell Culture Insert Plate found in: Nu-Serum™ IV Growth Medium Supplements, Nu-Serum™ Growth Medium Supplements, Falcon® Cell Culture Inserts, ThinCert™..
IncuCyte® NeuroPrime Cell Kit offers a complete solution for kinetic, live-cell imaging of 96-well co-culture assays utilizing primary rat neurons & astrocytes.
Conjugation, the transfer of DNA by direct cell-to-cell contact, depends on the presence of a conjugative plasmid(is small, double-stranded DNA molecules
Hierarchical tissues composed of spheroid and fiber structures such as tumors, embryos and glomeruli widely exist in organisms. Methods have been developed to build spheroid and fiber structures, independently, as tissue models in vitro. However, it is still a challenge to print them simultaneously and integrated f
The underlying technique our lab is built upon is a hematopoetic/mesenchymal stem cell coculture. In this system we are able to acurately recreate the bone marrow niche in-vitro, manipulate the microenvironment, and investigate the effects of such manipulations on the niche using fluorescence imaging, flow cytometry, in situ immunohistochemistry, and serial transplants.. ...
JGP study demonstrates how recordings from neuron-HEK cell cocultures provide a clearer picture of the factors involved in synaptic transmission.
BD Falcon FluoroBlok Cell Culture Inserts for Compound Permeability, Migration, and Invasion Assays : Permit real-time analysis of cell culture compo
High expression of B7-H3 on stromal cells defines tumor and stromal compartments in epithelial ovarian cancer and is associated with limited immune activation:
Get quotes fast and Choose from 2 clinic(s) offering Autologous Endometrial Coculture treatment in Turkey Get quotes fast & choose the best with phone numbers, reviews, prices, maps and pictures. | Page 0
Ultrastructure of control and GGF-treated cocultures. Schwann cell-DRG neuron cocultures that had myelinated for 3 wk were maintained for an additional 3 d with
Hi, Im having trouble quantifying the amount of RD cells on a membrane from a migration assay using transwells. When trying to quantify using an image with many cells, clusters get counted as one and I was wondering if t…
Purpose: : Evidences from literature shows that GITR-GITRL interaction plays important role in delivering co-stimulatory signals controlling regulatory T cells. Human GITR ligand has recently been shown to be constitutively expressed in retinal tissues. By expressing eYFP tagged human GITRL in human retinal pigment epithelial (RPE) cells, we investigated the biological significance of expression of GITRL on human ocular tissue. Methods: : Full length GITRL was amplified from a human ovarian cDNA library using GITRL specific primers and subcloned in a pEYFPC1 vector for expressing GITRL as an eYFP tagged protein in RPE cells. Using an RPE/T cell co-culture system we studied the invitro proliferative responses of purified CD3+ T cells or CD3+CD25-T cells co-cultured with lethally irradiated RPE cells in the presence or absence of anti-CD3 and anti CD28 antibody. For cytokine measurement, culture supernatants were collected from parallel RPE/T cell co-culture experiments. Results: : Both ...
Chronic Lymphocytic Leukemia (CLL) cells disseminate into supportive tissue microenvironments. To investigate the mechanisms involved in leukemic cell tissue retention we developed a 3D bone marrow (BM) microenvironment that recreates CLL - BM-stromal cells interactions inside a scaffold within a bioreactor. Our system allows the parallel analysis of CLL cells retained inside the scaffold and those released in the presence/absence of pharmacological agents, mimicking tissue and circulating cell compartments, respectively. CLL cells can be retained within the scaffold only in the presence of microenvironmental elements, which through direct contact down-regulate the expression of HS1 cytoskeletal protein in CLL cells. Consist with this, the expression of HS1 was lower in CLL cells obtained from patients BM versus CLL cells circulating in the PB. Moreover, we demonstrate that CLL cells with inactive-HS1, impaired cytoskeletal activity and a more aggressive phenotype are more likely retained ...
Epidemiological studies have linked obesity with basal-like breast cancer risk in both premenopausal and postmenopausal women, suggesting that obesity may affect tumor phenotype by skewing the microenvironment toward support of more aggressive tumor cell phenotypes (typically ER/PR−, HER-2−). In order to study the interactions between adipocytes and breast tumor cells, we developed an adipocyte-breast cancer cell co-culture system. The adipocytes SGBS (Simpson-Golabi-Behmel Syndrome) and human ER positive breast cancer MCF7 cells were co-cultured for 24 hours. Following co-culture, the cells from the inserts (MCF7 cells) and wells (SGBS cells) were collected separately and total RNA was then isolated. The mRNA levels of the NADPH oxidase subunit NOX4 and hypoxia-inducible factor 1α (HIF1α) were determined by RT-PCR and normalized to 18S RNA levels. When SGBS cells were co-cultured with MCF7 cells, SGBS NOX4 and HIF1α mRNA levels increased more than 10 fold and 3 fold, respectively, while ...
LY317615 (Enzastaurin) IC50 in tumors possess different metastasis potential. Although microfluidic technology provides better control over co-culture microenvironment, many systems insert hundreds or hundreds of cells in the gadget still, therefore they absence one cell quality as typical co-culture in petri meals.6C14 Although the solo cell co-culture on-chip allows for isolating solo cells in the step, there are even now two critical problems to be resolved: 1) Thanks to the small amount of secreted protein from solo cell, constant perfusion can easily wash apart the secretion and impair cell-cell interaction thus; and 2) As the system goals to research the heterogeneity of one cells, the chamber-chamber cross-talk, which can trigger unwanted connections, should end up being removed. In the prior functions reported on the one cell-cell connections15,16, the co-culture microenvironment of each cell group was not isolated completely. Hence, the cross-talk among different co-culture environments ...
In this study, we addressed the intratumoral cellular heterogeneity observed in SCCHN and identified differential expression of PD-L1 between subsets of tumor cells as a major mechanism of heterogeneous immunogenicity. More precisely, analysis of primary human SCCHN tumors and PDXs showed a striking correlation between the constitutive expression of CD44 and PD-L1 at the transcriptional and translational level. This was in agreement with published reports that previously noted PD-L1 expression to be quite heterogeneous within SCCHN tumors (15,16). Furthermore, our data from coculture experiments between autologous CD8+ T cells and purified CD44+ tumor cells demonstrated that this preferential expression of PD-L1 is biologically relevant.. Although blockade of the PD-1:PD-L1 axis significantly increased IFNγ production by CD8+ T cells cocultured with the CD44+ tumor cells, there was only partial restoration of IFNγ secretion to levels produced by CD8+ T cells cocultured with CD44− tumor ...
BACKGROUND: Stromal fibroblasts associated with in situ and invasive breast carcinoma differ phenotypically from fibroblasts associated with normal breast epithelium, and these alterations in carcinoma-associated fibroblasts (CAF) may promote breast carcinogenesis and cancer progression. A better understanding of the changes that occur in fibroblasts during carcinogenesis and
Additional file 2: Figure S2. of A human macrophage - hepatocyte co-culture model for comparative studies of infection and replication of Francisella tularensis LVS strain and subspecies holarctica and mediasiatica
LY317615 (Enzastaurin) IC50 in tumors possess different metastasis potential. Although microfluidic technology provides better control over co-culture microenvironment, many systems insert hundreds or hundreds of cells in the gadget still, therefore they absence one cell quality as typical co-culture in petri meals.6C14 Although the solo cell co-culture on-chip allows for isolating solo cells in the step, there are even now two critical problems to be resolved: 1) Thanks to the small amount of secreted protein from solo cell, constant perfusion can easily wash apart the secretion and impair cell-cell interaction thus; and 2) As the system goals to research the heterogeneity of one cells, the chamber-chamber cross-talk, which can trigger unwanted connections, should end up being removed. In the prior functions reported on the one cell-cell connections15,16, the co-culture microenvironment of each cell group was not isolated completely. Hence, the cross-talk among different co-culture environments ...
Video articles in JoVE about retinoblastoma protein include In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells, Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software, Analysis of Cell Cycle Position in Mammalian Cells, Induction of Protein Deletion Through In Utero Electroporation to Define Deficits in Neuronal Migration in Transgenic Models, Whole Mount Dissection and Immunofluorescence of the Adult Mouse Cochlea, In vitro Coculture Assay to Assess Pathogen Induced Neutrophil Trans-epithelial Migration, An In Vitro Model for Studying Cellular Transformation by Kaposi Sarcoma Herpesvirus, A Protein Preparation Method for the High-throughput Identification of Proteins Interacting with a Nuclear Cofactor Using LC-MS/MS Analysis, Genome-wide Analysis using ChIP to Identify Isoform-specific Gene Targets, Assessing Replication and Beta Cell Function in Adenovirally-transduced
Methods Human MSCs were transduced with a lentiviral vector containing TRAIL IRES-GFP under the control of a tetracycline dependent promoter. Successful transduction was measured using flow cytometry and immunoblotting. The biological activity of MSCTRAIL was determined using co-culture experiments. 5×105 MPM cells were stained withDiI and plated with 5×105 MSCTRAIL cells. After 24 h doxycycline (10 μg/ml) was added to induce TRAIL production and left for 48 h. Both cells and supernatant were collected and stained with Annexin V and DAPI to detect apoptosis and death respectively onflow cytometry. ...
Applying biological molecules from cell membranes to the surfaces of artificial materials is opening peepholes on the very basics of cell-to-cell interaction.
Glioblastoma (GBM) is a tumor of the central nervous system. After surgical removal and standard therapy, recurrence of tumors is observed within 6-9 months because of the high migratory behavior and the infiltrative growth of cells. Here, we investigated whether carnosine (β-alanine-l-histidine), which has an inhibitory effect on glioblastoma proliferation, may on the opposite promote invasion as proposed by the so-called
Bacterial mating or conjugation is the transfer of DNA from one bacterium to another via direct cell-to-cell contact through a mating pore. My current research uses the genetically-tractable bacterium Bacillus subtilis as a model system to explore the function and subcellular localization of a putative component of the bacterial mating pore apparatus. I have been characterizing the protein ConE (formerly YddE) which is encoded on the B. subtilis conjugal element ICEBs1. ConE is related to proteins encoded on conjugal elements in numerous bacteria, including the Gram-positive pathogens S. aureus, C. difficile, and L. monocytogenes. ConE belongs to a large superfamily of ATP-dependent pumps involved in the extrusion of proteins and DNA through membrane pores. I have shown that ConE and its ATPase domain are essential for mating of ICEBs1. In addition, ConE-GFP localizes at the cell poles, in close association with the membrane (see Figure). Given ConEs localization, ATPase domain, and ...
Bacterial mating or conjugation is the transfer of DNA from one bacterium to another via direct cell-to-cell contact through a mating pore. My current research uses the genetically-tractable bacterium Bacillus subtilis as a model system to explore the function and subcellular localization of a putative component of the bacterial mating pore apparatus. I have been characterizing the protein ConE (formerly YddE) which is encoded on the B. subtilis conjugal element ICEBs1. ConE is related to proteins encoded on conjugal elements in numerous bacteria, including the Gram-positive pathogens S. aureus, C. difficile, and L. monocytogenes. ConE belongs to a large superfamily of ATP-dependent pumps involved in the extrusion of proteins and DNA through membrane pores. I have shown that ConE and its ATPase domain are essential for mating of ICEBs1. In addition, ConE-GFP localizes at the cell poles, in close association with the membrane (see Figure). Given ConEs localization, ATPase domain, and ...
In the present work, we report evidence that in addition to its role on the proliferation of pancreatic progenitor cells, the mesenchyme is crucial in controlling the timing of pancreatic β-cell differentiation. When we cultured rat embryonic pancreatic epithelium in the absence of its surrounding mesenchyme, we found that Ngn3 expression was turned on rapidly and then turned off a few days later, in keeping with the reported in vivo pattern (15,25). Our filter-separation experiments indicate that the mesenchyme-induced delay in Ngn3 induction requires direct contact between the epithelium and the mesenchyme. This result fits in with recent data indicating that direct cell-cell contact between epithelial and mesenchymal cells suppresses β-cell formation (26). We also found that Ngn3 expression occurred far earlier without mesenchyme, within a few hours compared with 3 days with mesenchyme either in vitro or in vivo. This acceleration in Ngn3 induction resembles the pancreatic phenotype of mice ...
The present invention relates to a multicellular, three-dimensional, living mammalian tissue. The tissue is produced by a co-culture process wherein two distinct types of mammalian cells are co-cultured in a rotating bioreactor which is completely filled with culture media and cell attachment substrates. As the size of the tissue assemblies formed on the attachment substrates changes, the rotation of the bioreactor is adjusted accordingly.
The differential expression analysis revealed that the expression of 34 genes were significantly up-regulated in CAFs compared with those in NFs. Furthermore, the pathway analysis showed that cytoskeletal signaling pathway was up-regulated and TGFb1 played crucial roles as one of upstream regulators in CAFs. Real-time imaging showed that the motility of CAFs was significantly greater on extracellular matrix. Intriguingly, the motility of CAFs conferred invasiveness on GC cells co-cultured with CAFs. ...
J Immunol. of virus-host connection, we focused on KSHV-miR-K12-11 (miR-K12-11), unique among the -herpesviruses miRs in having an identical seed sequence with co-culture system we determined the viral oncogenic miR-K12-11 spreads into the extra cellular environment and shuttles into T cells, where it can reduce target gene manifestation and repress the IKK-dependent innate response to […]. ...
HYBRID REFRIGERATED CENTRIFUGE All in one unit from micro tube rotors to multi-place swing rotors. A reliable trump card for centrifuge... read more ...
This study demonstrates that the Corning 384-well spheroid microplate can be used to generate and perform viability assays with co-culture spheroids in an easy-to-use, high throughput format.
When I wrote about the NRDCs new Stewardship Index for Specialty Crops, and asked whether industrial monoculture was the real path to sustainable farming, the response from many of our readers was unsurprisingly
At the same clinic, he developed methods to treat male factor infertility using in vitro fertilization (IVF). He also co- ... He developed a precursor technique of Intracytoplasmic Sperm Injection (ICSI), which is now used for treatment of nearly all ... 1992). "Evaluation of Vero cell co-culture system for mouse embryos in various media". Human Reproduction. 7 (2): 276-80. doi: ... Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. Intracytoplasmic ...
... staining techniques and co-culturing using enrichment techniques, will allow proper cultivation and sequencing of TM7x cells. ... Using the medium enrichment technique can reduce the TM7x containing cultures from a complex community to a dual species co culture ... A stable co-culture of the TM7x and XH001 can be obtained by using a medium, which has been developed to resemble the saliva, ... The co-culture can be passed through a 28-gauge needle, after which it can be filtered using a 0.22 µm filter. ...
The technique will leverage computer assisted design models, co-culture media which support muscle and fat cells as well as ... Such a technique has already been perfected for industrial applications in manufacturing objects made out of plastic, nylon, ... Techniques of genetic engineering, such as insertion, deletion, silencing, activation, or mutation of a gene, are not required ... The major advantage is that this technique bypasses fetal bovine serum, meaning that no animal has to be killed in order to ...
Based on hypotheses about molecular regulators of valvular calcification and fibrosis, the lab uses in vitro co-culture systems ... It tries to identify though microgenomics techniques, the cellular and molecular differences in diseased vs. healthy heart ... Ongoing projects include utilization of novel biomaterials and measurement techniques, development of bioreactors, and ...
... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ... organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH E05.200.249.750 - tissue engineering MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ... patch-clamp techniques MeSH E05.200.750.132 - autoradiography MeSH E05.200.750.210 - bone demineralization technique MeSH ...
Immunological techniques are then commonly used to determine the species and/or serogroups of bacteria present in the sample. ... Although the plating method is quite specific for most species of Legionella, one study has shown that a coculture method that ... New techniques for the rapid detection of Legionella in water samples have been developed, including the use of polymerase ... "Isolation of Legionella anisa using an amoebic coculture procedure". Journal of Clinical Microbiology. 39 (1): 365-6. doi: ...
Isolated cell culture, specifically co-culture of testis tissue, has been a useful technique for examining the influences of ... There are also techniques which are not practical or feasible in vivo which can now be explored. In vitro work is not without ... The development of this culture technique has taken place mainly with the use of animal models e.g. mice or rat testis tissue. ... Various categories of infertile men may benefit from advances in these techniques, especially those with a lack of viable ...
Bhatia also used co-cultures of more than one cell type to prevent dedifferentiation of the liver cells, building on the work ... She successfully applied techniques from photolithography to petri dishes, to create a substrate that would support growth of a ... She and her coworkers have also used techniques from 3D printing to create a lattice of sugar as a framework for a synthetic ...
... is a technique of assisted reproductive technology. It involves placing a patient's fertilized ... Coculture can be an effective treatment for patients who have failed previous IVF cycles or who have poor embryo quality. A ... A typical Coculture cycle consists of the following steps: 1. Once a patient has been deemed an appropriate candidate for the ... The risks of Coculture are minimal. The procedure has been performed in over 1000 patients with no reported detrimental effects ...
The techniques are now used by many pregnant women and prospective parents, especially couples who have a history of genetic ... is co-cultured with a serum containing necessary signals, or is grafted in a three-dimensional scaffold to result. Embryonic ... It is a common technique to use mouse cells and other animal cells to maintain the pluripotency of actively dividing stem cells ... Weiss, Rick (2007-12-07). "Scientists Cure Mice Of Sickle Cell Using Stem Cell Technique: New Approach Is From Skin, Not ...
... scaffold techniques and scaffold-free techniques. Scaffold techniques include the use of solid scaffolds, hydrogels and other ... Spheroids co-cultured with tumor and healthy cells were used to simulate how cancerous cells interact with normal cells. ... Scaffold free techniques employ another approach independent from the use scaffold. Scaffold-free methods include e.g. the use ... These techniques have been applied to for in vitro disease models used to evaluate cellular responses to pharmaceutical ...
A variety of techniques has been developed for the isolation and culturing of amniotic stem cells. One of the more common ... Co-culturing, i.e. mixing cells and plating them together, of human amniotic stem cells with neonatal rat ventricular myocytes ... Mesenchymal stem cells (MSCs) are highly abundant in the amniotic fluid and several techniques have been described for their ... In general, these types of techniques are considered to be potentially significant but further investigations are required. ...
Genome-based analytical techniques may prove especially useful for L. anisa, as a study of isolates from various locations in ... La Scola B, Mezi L, Weiller PJ, Raoult D (2001). "Isolation of Legionella anisa Using an Amoebic Coculture Procedure". J Clin ... In addition, such techniques greatly reduce the time required to obtain results. Infections may be asymptomatic, and are ... However, some research suggests L. anisa may require a co-culture method that accounts for the close relationship with amoebae ...
He was a world authority on sex change operations, developing new techniques for transsexualism, embryo replacement, in vitro ... part of a team of clinicians and scientists which led to the world's first microinjection baby via human ampullary coculture[ ... and how he used cadavers to come up with his technique of sexual reassignment surgery. It also shows his assistant, Dr Lim, ...
Recently, exometabolomics has been used to design co-culture systems. Because the analysis of extracellular metabolites allows ... Footprinting, in combination with other techniques, for early recognition of outbreak and strain characterization. Studying ... Exometabolomic techniques have been used in the following fields: Metabolite utilization to annotate function of unknown genes ... "Liquid chromatography-mass spectrometry for metabolic footprinting of co-cultures of lactic and propionic acid bacteria". ...
Other microscopy techniques are commonly used in tandem with NanoSIMS that allow for multiple types of information to be ... NanoSIMS can be used for pure cultures, co-cultures, and mixed community samples. The first use of NanoSIMS in biology was by ... This technique can be used to study nutrient exchange. The mouse gut microbiome was investigated to determine which microbes ... Unlike other imaging techniques, where 13C14N and 12C15N cannot be independently measured due to nearly identical masses,, ...
Pre-implantation techniques include PGD, but also sperm sorting. Other assisted reproduction techniques include: Mitochondrial ... Autologous endometrial coculture is a possible treatment for patients who have failed previous IVF attempts or who have poor ... The latter technique has proved to be less deleterious for the embryo, therefore it is advisable to perform the biopsy around ... This technique is used in cases when mothers carry genes for mitochondrial diseases. The therapy is approved for use in the ...
The technique can be applied to a large number of cells to produce stem cells for medical purposes on an industrial scale. ... While initial studies revealed that co-culturing epithelial cells with Swiss 3T3 cells J2 was essential for CRC induction, with ... The technique enables researchers to search large libraries of antibodies and quickly select the ones with a desired biological ... Three techniques are widely recognized: Transplantation of nuclei taken from somatic cells into an oocyte (egg cell) lacking ...
This technique is used in cases when mothers carry genes for mitochondrial diseases. The therapy is approved for use in the ... Autologous endometrial coculture is a possible treatment for patients who have failed previous IVF attempts or who have poor ... Techniques usually used in in vitro fertilization include: *Transvaginal ovum retrieval (OVR) is the process whereby a small ... Less commonly used techniques in in vitro fertilization are: *Assisted zona hatching (AZH) is performed shortly before the ...
Clones of the S and L types could co-exist stably in co-culture with each other, indicating they occupied distinct niches in ... A later study by Leiby and Marx that used more advanced techniques showed that much of the decay Cooper and Lenski had ... and as technology and methodological techniques have advanced. The use of E. coli as the experimental organism has allowed many ... by Eric Quandt in the lab of Jeffrey Barrick at the University of Texas at Austin described the application of a new technique ...
In 2016, researchers developed a technique that used cancer cells' RNA to produce T cells and an immune response. They encased ... They then produce pure cultures of lymphocytes that can be tested for reactivity against other tumors, in coculture assays. ... As of 2015 the technique had expanded to treat cervical cancer, lymphoma, leukemia, bile duct cancer and neuroblastoma and in ...
This technique is also used in sheep, pigs and other animals. See In animals. Oocytes are classified depending on their ... Kannan S, Mehta A, Simha V, Reddy OK, Kaur BP, Onteru SK, Singh D (2014). "Photoinduction of granulosa cell and oocyte co-culture ... The best oocytes are chosen to be matured in the hope of then being implanted using in vitro fertilisation techniques. The ... IVM is not an entirely perfected technique. Pregnancy rates are lower in IVM than in standard IVF. There is also research ...
Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture ... This technique has demonstrated a stark difference in the sensitivity of the peripheral terminals compared to the neuronal cell ... After gelation, the agarose microgels will serve as a 3D microenvironment for cell co-culture. Segregated co-culture is also ... co-culture, and segregated (or indirect) co-culture. The use of conditioned media, where the cultured medium of one cell type ( ...
Optical detection includes fluorescence-based techniques, chemiluminescence-based techniques, and surface plasmon resonance ( ... Micropatterned co-cultures have also contributed to bio-MEMS for tissue engineering to recapitulate in vivo conditions and 3D ... Conventional micromachining techniques such as wet etching, dry etching, deep reactive ion etching, sputtering, anodic bonding ... Techniques for micropatterning paper include photolithography, laser cutting, ink jet printing, plasma treatment, and wax ...
Two major alternatives to in vivo animal testing are in vitro cell culture techniques and in silico computer simulation. ... circulatory flowrate and zonation and multi cellular co-cultures," the biochips have set themselves apart from basic cell ... One model is the "lung-on-a-chip". This combines microfabrication techniques with modern tissue engineering and mimics the ... 33 (439). Russell, W.M.S. and Burch, R.L., (1959). The Principles of Humane Experimental Technique, Methuen, London. ISBN 0- ...
Most significantly, the monolayers can be characterized with MALDI mass spectrometry in a technique known as SAMDI-MS, which ... Several strategies using this approach were studied in the context of cell signaling, migration, and co-culture. Subsequent ... Most recently, Mrksich's group has focused on developing a technique for assembling large molecular structures with perfectly ...
Prichard, JF; Thibodeaux, JK; Pool, SH; Blakewood, EG; Menezo, Y; Godke, RA (April 1992). "In-vitro co-culture of early stage ... and granulosa lutein cells to avoid microbial contamination during human oocyte retrieval for assisted reproductive techniques ...
Techniques for cell isolation depend on the cell source. Centrifugation and apheresis are techniques used for extracting cells ... Collagen/hyaluronic acid scaffolds have been used for modeling the mammary gland In Vitro while co-coculturing epithelial and ... This technique does not require the use of a solid porogen like SCPL. First, a synthetic polymer is dissolved into a suitable ... Each of these techniques presents its own advantages, but none are free of drawbacks. Molecular self-assembly is one of the few ...
Thus, a combination of the flap and AVB techniques can maximize the growth rate and vascular contact of the bioreactor as ... These cells secrete growth factors that promote repair and can be co-cultured with stem cells to improve tissue formation. ... Service, Robert F. (29 July 2005). "Technique Uses Body as 'Bioreactor' to Grow New Bone". Science. 309 (5735): 683. doi: ... A Novel Technique for Periosteal Harvest". Journal of Investigative Surgery. 17 (4): 229-233. doi:10.1080/08941930490472073. ...
This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal ... 7] Based on the evaluation of the babies born, no abnormalities attributable to the ROSI technique have been identified. [8][9] ... This technique can be used to enable genetic fatherhood to some men who have no spermatozoa in the ejaculate (azoospermia) and ... The technique was developed by Gianpiero Palermo at the Vrije Universiteit Brussel, in the Center for Reproductive Medicine ...
have co-cultured aquatic species for centuries in marine, brackish and fresh water environments.[1][3] Fish, shellfish and ... Ideally, the co-cultured species each yield valuable commercial "crops".[2] IMTA can synergistically increase total output, ... IMTA is a specialized form of the age-old practice of aquatic polyculture, which was the co-culture of various species, often ... Pacific SEA-lab is researching and is licensed for the co-culture of sablefish, scallops, oysters, blue mussels, urchins and ...
"Cost-effectiveness modelling of recombinant FSH versus urinary FSH in assisted reproduction techniques in the UK". Human ... Autologous endometrial coculture. *Cytoplasmic transfer. *Embryo transfer. *Gestational carrier. *In vitro maturation ...
The technique is recommended for couples who will not accept a child of the undesired gender.[27][28] ... The IVF/PGD technique is favored over the Ericsson method because of the stricter control of the offspring gender in the ... Sperm sorting is an advanced technique that sorts sperm "in vitro" by flow cytometry. This shines a laser at the sperm to ... Sperm sorting utilizes the technique of flow cytometry to analyze and 'sort' spermatozoa. During the early to mid-1980s, Dr. ...
Algae can be converted into various types of fuels, depending on the technique and the part of the cells used. The lipid, or ... Mackay, S.; Gomes, E.; Holliger, C.; Bauer, R.; Schwitzguébel, J.-P. (2015). "Harvesting of Chlorella sorokiniana by co-culture ... Using these techniques, the company states that lipid production could be increased several-fold, potentially making algal ... Algenol's technology produces high yields and relies on patented photobioreactors and proprietary downstream techniques for low ...
This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal ... The technique was developed by Gianpiero Palermo at the Vrije Universiteit Brussel, in the Center for Reproductive Medicine ... A systematic meta-analysis of 24 estimates of DNA damage based on a variety of techniques concluded that sperm DNA damage ... The first child born from a gamete micromanipulation (technique in which special tools and inverted microscopes are used that ...
Pregnancy urine dipstick tests are based on the lateral flow technique. *The urine test may be a chromatographic immunoassay or ... Autologous endometrial coculture. *Cytoplasmic transfer. *Embryo transfer. *Gestational carrier. *In vitro maturation ...
Ten Broek, R. P. G.; Kok- Krant, N.; Bakkum, E. A.; Bleichrodt, R. P.; Van Goor, H. Different surgical techniques to reduce ... 自体子宫内膜共同培养(英语:Autologous endometrial coculture) ... Gurgan T, Salman C, Demirol A. Pregnancy and assisted reproduction techniques in men and women after cancer treatment. Placenta ... a successful technique but with an accompanying high risk of hypertensive disorders during pregnancy. Hum. Reprod. March 2006, ...
IVF and related techniques (ICSI, ZIFT, GIFT) are called assisted reproductive technology (ART) techniques. ... ICSI technique is used in case of poor semen quality, low sperm count or failed fertilization attempts during prior IVF cycles ... This technique involves an injection of a single healthy sperm directly injected into mature egg. The fertilized embryo is then ... ART techniques generally start with stimulating the ovaries to increase egg production. After stimulation, the physician ...
... the findings showed reduced levels of tumor necrosis factor-alpha and interleukin-10 in T cell-microglia co-culture. The ... Peripherally inserted central catheter (Seldinger technique). *Central venous catheter. Other equipment. *Infusion pump ...
The co-cultured species should not just be biofilters, but harvestable crops of commercial value. A working IMTA system should ... including the development of modern broiler rearing techniques, the adoption of the Cornish Cross broiler, the use of laying ... result in greater production for the overall system, based on mutual benefits to the co-cultured species and improved ecosystem ...
However, they hardly form blooms with sufficiently high concentration to be visible without enrichment techniques. Purple ... "Photoproduction of H2 from acetate by syntrophic cocultures of green sulfur bacteria and sulfur-reducing bacteria". Archives of ...
Co-cultures consisting of pericytes and endothelial cells induce the expression of TIMP-3 by pericytes, while endothelial cells ... Examination of cathepsin activity by using chemical probes and in vivo imaging techniques demonstrated an increase in cathepsin ...
These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid ... Duell BL, Cripps AW, Schembri MA, Ulett GC (2011). "Epithelial cell coculture models for studying infectious diseases: benefits ... This technique is known as two-dimensional (2D) cell culture, and was first developed by Wilhelm Roux who, in 1885, removed a ... The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the ...
This is one potential distinction from the age-old practice of aquatic polyculture, which could simply be the co-culture of ... "Aquaculture's growth continuing: improved management techniques can reduce environmental effects of the practice. (UPDATE)." ... Onshore recirculating aquaculture systems, facilities using polyculture techniques, and properly sited facilities (for example ... A working IMTA system can result in greater total production based on mutual benefits to the co-cultured species and improved ...
Therefore, a number of techniques are employed by microbiologists when culturing anaerobic organisms, for example, handling the ... "Isolation and culture of Methanobrevibacter smithii by co-culture with hydrogen-producing bacteria on agar plates". Clinical ... bacteria in a glovebox filled with nitrogen or the use of other specially sealed containers, or techniques such as injection of ...
More recently, however, studies in 3D human coculture are proving to be an effective method with which to study cancer risks in ... Novel multicolor fluorescence painting techniques of human chromosomes have clearly demonstrated that high-LET α-particles and ...
In order to properly seed the 2D cell culture, a microcontact printing technique was used to lay out a fibronectin "brick wall ... Hermanns MI, Fuchs S, Bock M, Wenzel K, Mayer E, Kehe K, Bittinger F, Kirkpatrick CJ (April 2009). "Primary human coculture ... For example, animal models are often subjected to mechanical or chemical techniques that simulate human injuries. There are ... conventional laboratory techniques usually focus on 2D structures, such as petri-dish that lacks capability to recapitulate ...
Emerging optic-based techniques, for example, the pH optrode (or optode), which can be integrated into a self-referencing ... "Membrane potential depolarization causes alterations in neuron arrangement and connectivity in cocultures". Brain and Behavior ... These techniques allow further characterization of the wound electric fields/currents at cornea and skin wounds, which show ... The gold standard techniques to quantitatively extract electric dimensions from living specimens, ranging from cell to organism ...
This study demonstrated that both the monoculture extracts of the native strain T. maxima and its co-culture with P. carneus ... recovery of gold from electronic waste using mycofiltration techniques. Fungi are amongst the primary saprotrophic organisms in ... Chan-Cupul W, Heredia-Abarca G, Rodríguez-Vázquez R (2016). "Atrazine degradation by fungal co-culture enzyme extracts under ... a prospective environmental friendly technique of bioseparation and dewatering of domestic wastewater sludge". Environmental ...
Psychiatry related information on Coculture Techniques. *The von Ebners-like protein mRNA was induced after 4 h of co-culture ... Chemical compound and disease context of Coculture Techniques. *The coculture of cells expressing the HIV-1 envelope ... Gene context of Coculture Techniques. *Additionally, in cocultures in which spleen cells were derived from receptor-deficient ... Disease relevance of Coculture Techniques. *Antiestrogen-induced TGF-beta from MCF-7 cells inhibits the growth of an estrogen ...
The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and ... The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and ...
Results showed that cocultured cells expressed much higher N-cadherin than monocultured cells after 24 and 48 h of culture. We ... Coculture Techniques * Endothelium, Vascular / cytology * Endothelium, Vascular / physiology* * Humans * Middle Aged * ... In addition, more beta-catenin was found to translocate into the cocultured cells nuclei and more T cell factor-1 (TCF-1) were ... Taking our findings together it can be concluded that cocultures of HBMSCs with HUVECs increased N-cadherin expression and ...
... J Neurochem. 2011 Nov;119(3 ... To this end, differentiated rat NPCs were co-cultured with rat BMECs and shown to induce BBB properties such as elevated trans- ... Coculture Techniques * Endothelium, Vascular / cytology* * Endothelium, Vascular / physiology* * Humans * Male * ... Taken together, these results demonstrate that NPCs are an attractive alternative to primary neural cells for use in BBB co-culture ...
Coculture Techniques. Ganglia, Sensory / cytology*. Intracellular Space / drug effects, metabolism. Neurons / cytology, drug ... Calcium sensitivity of petrosal ganglion neurons (PGNs) to chemical stimuli with and without PC-12 cells in co-culture instead ...
Conventional G-banding technique was used to analyze parental MG63, MGRWV, MGLN, and MGC4-2 cells. The cytogenetics of MG63 ... Three-dimensional coculture of PCa and bone stromal cells in RWV. LN, C4-2, MG-63, HFOB, and HS27A cells were seeded in T- ... Cocultured organoids were harvested after 2 or 3 wk with dispase (5 mg/mL; Roche) or trypsin (0.25% v/v; Invitrogen). Cells ... We cocultured MG63, a human osteosarcoma cell line, or HS27A, a normal immortalized human osteoblastic cell line, with LN and ...
Molecular techniques.DNA and RNA extractions from cheese samples (0.5 g each), PCR and reverse transcription (RT)-PCR ... Production of freeze-dried kefir coculture.Kefir coculture isolated from a commercial kefir drink was used in the present study ... Addition of a freeze-dried kefir starter coculture, the initial concentration of the freeze-dried kefir coculture, the salting ... Evaluation of Freeze-Dried Kefir Coculture as Starter in Feta-Type Cheese Production. Y. Kourkoutas, P. Kandylis, P. Panas, J. ...
A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering, Isolation and Culture of ... culture techniques include Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow, ... Cell Co-culture Patterning Using Aqueous Two-phase Systems. John P. Frampton1, Joshua B. White1, Abin T. Abraham1, Shuichi ... Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions. Ayşegül Doğan1, ...
We cocultured SVCs that include macrophages with adipose tissue from lean C57BL/6J mice in the presence of an adrenergic ... Tissues were handled using sterile techniques and minced into fine (,10 mg) pieces. For SVC isolation, minced samples were ... Coculture experiments. To isolate SVCs, perigonadal adipose tissue was isolated from high-fat diet-induced obese C57BL/6J male ... SVCs were cocultured with adipose tissue pieces for 24 hours in DMEM containing 10% FBS, 1% penicillin, and 2% fatty acid-free ...
A) 3-wk-old myelinating OL-DRG cocultures stained for MBP, Olig2, and neurofilament. Coc ... Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. ( ... Coculture Techniques. *Cytoskeleton/metabolism. *Ganglia, Spinal/cytology. *Heterocyclic Compounds with 4 or More Rings/ ... fig5: Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. (A) 3-wk-old myelinating OL-DRG cocultures stained ...
Adherent MN were cocultured in the presence of varying concentrations of 1α,25(OH)2D3 in 0.1% ethanol for 72 h. Staining for ... Infection and subsequent handling of cultures was according to techniques described previously (31). MN preparations were ... 1⇑B). To investigate the relationship between RLU and CFU, we cocultured the PBMC of six donors with MTB-lux at a MOI of 0.1:1 ... The coculture of BCG-lux with PBMC is a convenient assay system, but we wished to ensure our observations were generalizable to ...
1.Laboratoire Mer Molécules Santé (MMS), FR CNRS 3473 IUML, Faculté des Sciences et TechniquesUniversité du MaineLe MansFrance ... Allelopathic tests were realized by co-culturing these microalgae with H. ostrearia in batch and in semi-continuous mode, based ... 3.Laboratoire Mer Molécules Santé, FR CNRS 3473 IUML, Faculté des Sciences et TechniquesUniversité de NantesNantesFrance ... Does allelopathy affect co-culturing Haslea ostrearia with other microalgae relevant to aquaculture?. ...
Autologous Endometrial Coculture is a technique of assisted reproductive technology. It involves placing a patients fertilized ... Coculture can be an effective treatment for patients who have failed previous IVF cycles or who have poor embryo quality. A ... A typical Coculture cycle consists of the following steps: 1. Once a patient has been deemed an appropriate candidate for the ... The risks of Coculture are minimal. The procedure has been performed in over 1000 patients with no reported detrimental effects ...
... but did not protect co-cultures, when used alone or in combination. Application of GSSG prevented degeneration in co-cultures, ... Cell viability was measured biochemically and by quantitative neurite imaging, siRNA techniques were also used. We initially ... In these co-cultures, MPTP was metabolized to 1-methyl-4-phenyl-pyridinium (MPP(+) ) by the glial cells, and the toxic ... Moreover, available test systems rarely reflect functional roles of co-cultured glial cells. There is no human in vitro ...
Co-culture of MEFs and HUVECs by encapsulating them together in 0.07% RAD16-I. Molecular Biology Techniques. RT-PCR. Western ... Co-culture of MEFs and HUVECs. Co-culture of MEFs and HUVECs by loading a HUVECs suspension over a MEFs culture in 0.07% RAD16- ... Co-culture MEFs + HUVECs. HUVECs loaded over MEFs. Co-culture MEFs + HUVECs. HUVECs loaded over MEFs. Collagen X Expression. WB ... Analysis of the Gene Expression of Co-Cultures of MEFs and HUVECs in RAD16-I Ortega et al. 2004. Coll I. Coll I. Coll II. Coll ...
Coculture Techniques. Female. Male. Nerve Regeneration. Neurites / physiology. Olfactory Nerve / cytology*, immunology, ... Rolf B1.T cells support the regrowth of neurites from adult retinal ganglion cells in vitro in a heterologous co-culture system ...
Co-culture experiments that exposed healthy donor PBMCs to lung cancer cells in vitro were performed to evaluate the effect on ... Further mechanism dissection showed that co-culturing T cells with RCC cells could produce more IGF-1 and FGF-7, which may ... Mechanism dissection suggested that co-culturing T cells with RCC cells released more T cell attraction factors, including IFN- ... induced similar changes in cytokine gene and protein expression by healthy donor PBMCs in an in vitro co-culture system. We ...
Thus, amoebal coculture allows the recovery of new bacterial species from heavily contaminated samples and might be a valuable ... Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human ... To isolate new ARB, we performed amoebal coculture from 444 nasal samples. We recovered 7 (1.6%) ARB from 444 nasal swabs, ... using that technique, Rowbotham was able to grow L. pneumophila from human feces (43). Moreover, the amoebal coculture is a ...
An internal reference technique for accurately quantifying specific mRNAs by real-time PCR with application to the tceA ... S1 in the supplemental material); therefore, cocultures were maintained with 0.6 mM TCE and 4 mM butyrate. After this coculture ... mccartyi-containing cocultures (4.2 to 6.8 μmol day−1 [5, 7, 14-16]). The cell yield of strain 195 in this coculture [(1.1 ± ... in the cocultures growing on butyrate. In contrast, when the coculture was maintained on crotonate, the cell number ratio was ...
Pre-degenerated peripheral nerves co-cultured with bone marrow-derived cells: a new technique for harvesting high-purity ...
Retinal Coculture Techniques. A new review discusses retinal coculture models of the neural retina, retinal pigment epithelium ...
Spinal cord - motor cortex co-culture model - a new technique to study neuronal regeneration in vitro Meeting Abstract ... Conclusions: This new slice co-culture model provides an important in vitro tool for a variety of questions in the field of ... We report here about a new slice co-culture model for the study of neuronal regeneration. ...
Coculture Techniques. 2. 2014. 1346. 0.090. Why? Gene Knockdown Techniques. 1. 2012. 1689. 0.090. Why? ...
Two different cell populations can be labeled with different ViaFluor® SE dyes, mixed together, and tracked in co-culture over ... For two-color co-culture, stain each population separately with a different ViaFluor® dye color. ... Luminescence: Mechanisms and Applications for 4 Prominent Techniques Subscribe & Follow. Sign up for news & promotions:. ...
21st National level training programme on In-Vitro Fertilization and Embryo Co-Culture: TANUVAS, Chennai, India. Feb 24 to Mar ... Department lab rotation to understand advanced reproductive techniques in the filed of Reproductive Biology. (CASA, Hamster ... Preparation and maintenance of oviduct epithelial cells, cumulus cells etc., for embryo co-culture. ... "In-Vitro fertilization and Embryo Co-Culture" for 21 days starts from Feb 24 to Mar 15, 2016. ...
18th National level training programme on In-Vitro Fertilization and Embryo Co-Culture: TANUVAS, Chennai, India. Aug 01, 2014 ... Department lab rotation to understand advanced reproductive techniques in the filed of Reproductive Biology. (CASA, Hamster ... Preparation and maintenance of oviduct epithelial cells, cumulus cells etc., for embryo co-culture. ... "In-Vitro fertilization and Embryo Co-Culture" for 21 days starts from Aug 01,2014 to Aug 23, 2014. ...
24.2.4 Patterned Co-cultures Using LbL Technique 534. 24.3 Controlled Drug Delivery for Nerve Regeneration 536 ... In the last 15 years the LbL technique has seen considerable developments and has now reached a point where it is beginning to ... The book gives a thorough overview of applications of the LbL technique in the context of bioengineering and biomedical ... 14.2 Fabrication of Polysaccharide Capsules by the LbL Technique 296. 14.2.1 Natural Charged Polysaccharides Used in LbL ...
Advanced training in cell culture and co-culturing techniques. *Immunohistochemical and immunofluorescence staining techniques ... Opportunities to learn RNA, DNA and protein assessment techniques. * ... as well as endocrine and neurotoxicology techniques. ...
Cell-culture-techniques; Cell-cultures; Lung-cells; Animals; Humans; Laboratory-animals; Author Keywords: Coculture; Gene ... As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer ... expressed in both cell types in coculture than in monoculture. When reduced to only those genes involved in inflammation and ... induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene ...
Confocal laser scanning analysis of coculture experiments of A818-6 cells with Kif-5 fibroblasts transduced with the gene ... Fluorescent Antibody Technique. *Growth Substances/pharmacology. *Humans. *Morphogenesis/drug effects. *Protein Isoforms/ ... Mentions: Fibroblast cocultures with A818-6 cells were set up using foreskin-derived KIF-5 fibroblasts. When A818-6 cells were ... Mentions: Fibroblast cocultures with A818-6 cells were set up using foreskin-derived KIF-5 fibroblasts. When A818-6 cells were ...
  • One brain cell line was partially characterized by DNA sequencing, morphology, antigen expression using flow cytometry, confocal microscopy, and electrophysiology using the patch clamp technique. (mdc-berlin.de)
  • This study compared global multi-walled carbon nanotube (MWCNT)-induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene expression from mouse lungs exposed to MWCNT. (cdc.gov)
  • Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure. (cdc.gov)
  • This study identified differential gene regulation in human small airway epithelial cells (SAECs) grown either in monoculture or in coculture with human microvascular endothelial cells following exposure of the SAECs to multiwalled carbon nanotubes (MWCNTs). (cdc.gov)
  • This study is based on an in vitro coculture model consisting of outgrowth endothelial cells and primary osteoblasts and is currently being used in different studies of bone repair processes with special regard to angiogenesis and osteogenesis. (hindawi.com)
  • Coculture systems of OECs and pOBs positively influence the angiogenic potential of endothelial cells by inducing the formation of angiogenic structures in long-term cultures. (hindawi.com)
  • In the present study a coculture system of hepatocytes and endothelial cells was established to investigate the communication between parenchymal and nonparenchymal cells. (elsevier.com)
  • In vitro fertilization with coculture has been utilized in animal in vitro embryo culture systems for over 30 years and more recently in some clinical human in vitro fertilization laboratories as well. (advancedfertility.com)
  • Some studies have demonstrated improved pregnancy rates and delivery rates with utilization of coculture for human in vitro fertilization. (advancedfertility.com)
  • There is not universal agreement that coculture is necessary to provide optimal pregnancy rates from human in vitro fertilization. (advancedfertility.com)
  • One school says that coculture can be of a benefit for some patients undergoing in vitro fertilization. (advancedfertility.com)
  • Clinical in vitro fertilization programs that are utilizing coculture for their human IVF generally use one of three cell types. (advancedfertility.com)
  • citation needed] In 1982, Cohen joined Bourn Hall Clinic as an Embryologist, working with Patrick Steptoe and Robert G. Edwards on techniques geared towards improving human conception through in vitro fertilization (IVF). (wikipedia.org)
  • The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels. (asm.org)
  • These results, combined with our previous observations, support the concept of permanent genetic and behavioral changes of PCa epithelial cells after being either cocultured with prostate or bone stromal cells as three-dimensional prostate organoids or grown as tumor xenografts in mice. (aacrjournals.org)
  • Primary CLL cells were cocultured in vitro with CD40L-expressing stroma to mimic the prosurvival conditions present in lymphoid tissue. (aacrjournals.org)
  • Lentiviral infection of purified OPC cultures resulted in significant cell death (unpublished data), whereas infection of OPC in cocultures with DRG neurons was well tolerated. (nih.gov)
  • Incubation of cocultures with lentivirus resulted in the preferential infection of OPC rather than neurons, as indicated by GFP expression (Fig. S3, available at http://www.jcb.org/cgi/content/full/jcb.200802091/DC1). (nih.gov)
  • In contrast, the presence of L1-Fc, the extracellular portion of a contactin ligand expressed on axons, enhanced survival and additionally promoted myelination in cocultures of neurons and oligodendrocytes. (jneurosci.org)
  • Another issue is that depending on the source of cells used for the coculture there are concerns about transmission of infectious diseases such as viruses from the cell line to the developing embryos. (advancedfertility.com)
  • Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. (wikipedia.org)
  • Autologous Endometrial Coculture is a technique of assisted reproductive technology. (wikipedia.org)
  • Human bone stromal cells, after three-dimensional coculture with human prostate cancer (PCa) cells in vitro , underwent permanent cytogenetic and gene expression changes with reactive oxygen species serving as mediators. (aacrjournals.org)
  • In this communication, we showed marked cytogenetic, gene expression and behavioral changes in bone cells cocultured under three-dimensional conditions with PCa cells, with ROS serving as the critical mediators. (aacrjournals.org)
  • Using a cutoff of 10% false discovery rate and 1.5 fold change, we determined that there were more concordant genes (gene expression both up- or downregulated in vivo and in vitro) expressed in both cell types in coculture than in monoculture. (cdc.gov)
  • As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer enhanced in vitro models for nanoparticle risk assessment and the reduction of in vivo toxicological testing. (cdc.gov)
  • The direction and fold-change of this differential gene regulation suggests that toxicity testing in monoculture may exaggerate cellular responses to MWCNTs, and coculture of cells may provide a more in-depth assessment of toxicological responses. (cdc.gov)
  • Tissue engineering is considered to be the intracellular regulation by gene transduction and the extracellular regulation by culture methods, such as coculture. (osti.gov)
  • When reduced to only those genes involved in inflammation and fibrosis, known outcomes of in vivo MWCNT exposure, there were more disease-related concordant genes expressed in coculture than monoculture. (cdc.gov)
  • SAEC genes that either changed their regulation direction from upregulated in monoculture to downregulated in coculture (or vice versa) or had a more than a two-fold changed in the same regulation direction were identified. (cdc.gov)
  • Knockdown of MLC in OPC-DRG cocultures resulted in a significant increase in myelination similar to that observed in cultures treated with blebbistatin. (nih.gov)
  • The student will learn several state-of-the art techniques, including droplet microfluidics, enzymatic hydrogel crosslinking, (3D) stem cell culture, immunohistochemistry, and confocal fluorescence imaging and apply this skill set in a top-level institute to perform pioneering work on artificial cells. (utwente.nl)
  • GE Healthcare is sponsoring a new, free educational webinar , "Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles in Spheroids," which will discuss magnetic 3D bioprinting of spheroids and rings, offer tips for high-throughput confocal imaging of 3D cells within Greiner Bio-One 384 well black µClear® flat bottom cell-repellent surface microplates, techniques for bioprinting cell co-cultures, and rapid 3D model formation with easy post-culture analysis. (prweb.com)
  • While single-cell models of pulmonary exposure are often used to determine the potential toxicity of nanomaterials in vitro, previous studies have shown that coculture cell models better represent the cellular response and crosstalk that occurs in vivo. (cdc.gov)
  • Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. (springer.com)
  • We conclude that structured cocultures of cardiomyocytes and fibroblasts mimic in vivo ventricular tissue organization and provide a novel tool for in vitro research into cardiac electromechanical function. (surrey.ac.uk)
  • Microstructured cocultures of cardiac myocytes and fibroblasts: a two-dimensional in vitro model of cardiac tissue. (surrey.ac.uk)
  • We used microfluidic techniques to grow two-dimensional structured cardiac tissue models, containing both myocytes and fibroblasts, and characterized cell morphology, distribution, and coupling using immunohistochemical techniques. (surrey.ac.uk)
  • During coculture M. haemolytica growth was inhibited when B. trehalosi entered stationary phase (6 h) resulting in a final cell density for M. haemolytica that was 6 to 9 logs lower than expected with growth in the absence of B. trehalosi . (pubmedcentralcanada.ca)
  • Coculture supernatant failed to inhibit M. haemolytica growth on agar or in broth, indicating no obvious involvement of lytic phages, bacteriocins, or quorum-sensing systems. (pubmedcentralcanada.ca)
  • In addition, to gain a better understanding on the origin of different growth factors, both direct and indirect coculture strategies were employed. (hindawi.com)
  • This study is the first one to successfully use modern chemical-analytic techniques to visualize the spatial distribution of metabolites and in this way explain the growth of a bacterial colony consisting of cooperating bacteria. (phys.org)
  • Therefore, techniques for the accurate fabrication of multifunctional scaffolds are needed. (biomedcentral.com)
  • Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human pathogens. (cdc.gov)
  • Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB) and clean the samples of other more rapidly growing species that generally overwhelm the agar plates. (cdc.gov)
  • Explorative studies are needed to identify yet undiscovered reservoirs and transmission routes for Legionella bacteria, and improved laboratory techniques are needed to detect Legionella spp. (cdc.gov)
  • Limitations in standard culture (overgrowth by other bacteria/resilience of microorganisms to standard culture techniques) mean standard culture may not detect the diversity of bacteria present in non-cystic fibrosis bronchiectasis. (bmj.com)
  • Here we used several metabolomic approaches to examine the chemicals made by these bacteria when grown in pairwise coculture. (asm.org)
  • We observed that N-cadherin concentrated in the membrane of cocultured HBMSCs (co-HBMSCs) while distributed within the cytoplasm of monocultured HBMSCs, which indicated that the cell-cell adhesion was improved between cocultured cells. (nih.gov)
  • Coculture with agonists or antagonists of the membrane or nuclear vitamin D receptors indicated that these effects were primarily mediated by the nuclear vitamin D receptors. (jimmunol.org)
  • Suturing techniques, as well as the usage of human amniotic membrane "bandages" were evaluated. (diva-portal.org)
  • Coculture of human prostate or bone stromal cells with human PCa LNCaP (LN) cells under three-dimensional conditions induced permanent genetic, morphologic, and behavioral changes in LN cells ( 4 ). (aacrjournals.org)
  • Stereolithography and fused filament fabrication techniques enable the 3D modeling of cancer cell metastasis to bone tissue. (corning.com)
  • Thus, amoebal coculture allows the recovery of new bacterial species from heavily contaminated samples and might be a valuable approach for the recovery of as-yet unrecognized emerging pathogens from clinical specimens. (cdc.gov)
  • Clinical studies have supported the efficacy of a variety of osteopathic manipulative techniques. (jaoa.org)
  • Data from the present study suggest that fibroblast proliferation and expression/secretion of proinflammatory and anti-inflammatory interleukins may contribute to the clinical efficacy of indirect osteopathic manipulative techniques. (jaoa.org)
  • There are currently no whole body imaging techniques in clinical use that are capable of specifically identifying bacterial infections. (spie.org)
  • Current clinical culture techniques use an a priori decision on which organisms are likely to be present/important as causative infectious agents. (bmj.com)
  • Novel biomaterials, artificial organ systems, cellular therapy and useful materials were investigated for utilizing the regulation techniques of cell proliferation. (osti.gov)
  • Dehalococcoides mccartyi 195 (strain 195) and Syntrophomonas wolfei were grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. (asm.org)
  • The upgrade would rely on a technique known as "co-culturing," in which different types of cells are grown together. (scientificamerican.com)
  • Results showed that cocultured cells expressed much higher N-cadherin than monocultured cells after 24 and 48 h of culture. (nih.gov)
  • Building upon previous expertise, this project will employ a combination of microfabrication and surface functionalisation techniques to create an electroanalytical microdevice with spatially controlled vascular cell populations. (strath.ac.uk)
  • The project will provide the opportunity to develop expertise in a wide range of both engineering and cell biology techniques, including microfabrication and lab-on-a-chip techniques, electrochemical sensors, vascular cell isolation and culture, and live-cell imaging. (strath.ac.uk)
  • Recent microfabrication techniques, such as hydrogel modeling has facilitated the development of vascular network models in vitro. (aiche.org)
  • Additionally, understanding tooth formation can lead to advances in dentistry, leading to improved restoration techniques. (tufts.edu)
  • To characterize some of the cell-cell connections that may be important for MO differentiation we cocultured human MO with lymphocytes and/or with platelets. (uni-regensburg.de)
  • Non-autologous cell lines should be screened for infectious diseases prior to use in human embryo coculture. (advancedfertility.com)
  • To investigate human fibroblast proliferation and interleukin secretory profiles in response to modeled repetitive motion strain (RMS) and modeled indirect osteopathic manipulative techniques (IOMT). (jaoa.org)
  • Coculture involves a lot of tedious work in the laboratory which leads to additional expense. (advancedfertility.com)
  • However, these protocols all require culture at very low densities and cell-cell/matrix interactions for neuronal differentiation, and the mechanisms underlying neuronal lineage commitment induced by these techniques remain unclear. (biologists.org)
  • Assisted Hatching (AH) is another commonly applied technique aimed at increasing implantation rates among infertile couples. (wikipedia.org)
  • Previous evidence supportsthe potential for direct chondrogenic differentiation of stem cells by coculturing with a terminally differentiated chondrocyte cell population ( 11 , 12 ). (pnas.org)
  • Although this review is focused mainly on cancer and particularly melanoma, the discussed techniques are in general applicable to broad research field of biology and medicine, including stem cells, development, aging, immunology and intercellular communication. (mdpi.com)
  • The use of kefir coculture as a starter led to increased lactic acid concentrations and decreased pH values in the final product associated with significantly higher conversion rates compared to salted rennet cheese. (asm.org)
  • Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. (asm.org)
  • High-throughput enrichments were set up with the MicroDrop technique using an alpine calcareous soil sample and a mixture of polymeric carbon compounds supplemented with signal compounds. (nih.gov)
  • With recent improvements in IVF culture media and techniques, our IVF pregnancy rates (without coculture) have improved dramatically. (advancedfertility.com)
  • They believe that this can yield an equally high pregnancy rate without the use of coculture. (advancedfertility.com)
  • The present study focused on the investigation of the roles of neural (N)-cadherin in early differentiation of HBMSCs in direct-contact cocultures with HUVECs for 24 and 48 h. (nih.gov)
  • However, the mechanisms by which ES cells are committed to the neural lineage using this technique are poorly understood. (biologists.org)
  • The use of cell cultures to test the biocompatibility of drugs, biomaterials or treatment techniques used in various disciplines is gaining in importance. (egms.de)
  • While complete regeneration of corneal tissues occurred, only slight differences in effects of surgical technique could be found. (diva-portal.org)
  • The book gives a thorough overview of applications of the LbL technique in the context of bioengineering and biomedical engineering where the last years have witnessed tremendous progress. (wiley.com)
  • Ann alternative for the control of virus diseases is the development of resistant plants through genetic transformation techniques. (usp.br)
  • Nano3D Biosciences and MD Anderson have developed a magnetic 3D bioprinting technique to successfully print spheroids and contracting ring structures that model wound healing. (prweb.com)
  • 4. Various standard tissue dissociation techniques will be used, alone or in combination, to generate new insect cell lines. (usda.gov)
  • Cell viability was measured biochemically and by quantitative neurite imaging, siRNA techniques were also used. (sigmaaldrich.com)
  • He developed a precursor technique of Intracytoplasmic Sperm Injection (ICSI), which is now used for treatment of nearly all male factor infertility diagnoses. (wikipedia.org)
  • Included are basic RT-PCR techniques, cell culture systems, second-messenger signaling methods, and patch-clamp techniques. (indigo.ca)