A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.
Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.
Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).
Cellular signaling in which a factor secreted by a cell affects other cells in the local environment. This term is often used to denote the action of INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS on surrounding cells.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.
Methods for maintaining or growing CELLS in vitro.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Benzoic acid or benzoic acid esters substituted with one or more bromine atoms.
Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.
Established cell cultures that have the potential to propagate indefinitely.
Technique for treating DEHYDRATION and WATER-ELECTROLYTE IMBALANCE by subcutaneous infusion of REHYDRATION SOLUTIONS.
Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.
Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.
Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.
Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.
Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.
A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)
A pair of highly specialized muscular canals extending from the UTERUS to its corresponding OVARY. They provide the means for OVUM collection, and the site for the final maturation of gametes and FERTILIZATION. The fallopian tube consists of an interstitium, an isthmus, an ampulla, an infundibulum, and fimbriae. Its wall consists of three histologic layers: serous, muscular, and an internal mucosal layer lined with both ciliated and secretory cells.
The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.
Adherence of cells to surfaces or to other cells.
The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.
Progenitor cells from which all blood cells derive.
A cell line derived from cultured tumor cells.
Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.
Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.
A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.
One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.
A family of anaerobic, coccoid to rod-shaped METHANOBACTERIALES. Cell membranes are composed mainly of polyisoprenoid hydrocarbons ether-linked to glycerol. Its organisms are found in anaerobic habitats throughout nature.
The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.
Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (1/7795)

Interleukin-12 (IL-12) is a potent antitumor cytokine, which induces and enhances the activity of natural killer (NK) cells, lymphokine activated killer (LAK) cells and cytotoxic T lymphocytes (CTL). IL-12 also stimulates IFN-gamma production from both T cells and NK cells. In this study, we transfected methylcholanthrene-induced fibrosarcoma (MCA-D) with TNF gene and investigated the therapeutic effect of TNF gene-transduced cancer vaccine and whether the vaccination effect is enhanced by systemic administration of recombinant IL-12 (rIL-12), in a murine model. TNF gene-transduced cancer vaccine or systemic administration of rIL-12 showed slight or moderate inhibition of pre-established tumor. However, simultaneous application of the vaccine and rIL-12 resulted in complete eradication. The cytotoxicity of CTL against parental tumor cells was enhanced with the combination of the vaccine and rIL-12, and IFN-gamma production from spleen cells also increased synergistically. Our findings show that synergistic enhancement of CTL activity and IFN-gamma production could play an important role in the antitumor effect of combination therapy using TNF gene-transduced cancer vaccine and rIL-12.  (+info)

Regulation of neurotrophin-3 expression by epithelial-mesenchymal interactions: the role of Wnt factors. (2/7795)

Neurotrophins regulate survival, axonal growth, and target innervation of sensory and other neurons. Neurotrophin-3 (NT-3) is expressed specifically in cells adjacent to extending axons of dorsal root ganglia neurons, and its absence results in loss of most of these neurons before their axons reach their targets. However, axons are not required for NT-3 expression in limbs; instead, local signals from ectoderm induce NT-3 expression in adjacent mesenchyme. Wnt factors expressed in limb ectoderm induce NT-3 in the underlying mesenchyme. Thus, epithelial-mesenchymal interactions mediated by Wnt factors control NT-3 expression and may regulate axonal growth and guidance.  (+info)

Reciprocal control of T helper cell and dendritic cell differentiation. (3/7795)

It is not known whether subsets of dendritic cells provide different cytokine microenvironments that determine the differentiation of either type-1 T helper (TH1) or TH2 cells. Human monocyte (pDC1)-derived dendritic cells (DC1) were found to induce TH1 differentiation, whereas dendritic cells (DC2) derived from CD4+CD3-CD11c- plasmacytoid cells (pDC2) induced TH2 differentiation by use of a mechanism unaffected by interleukin-4 (IL-4) or IL-12. The TH2 cytokine IL-4 enhanced DC1 maturation and killed pDC2, an effect potentiated by IL-10 but blocked by CD40 ligand and interferon-gamma. Thus, a negative feedback loop from the mature T helper cells may selectively inhibit prolonged TH1 or TH2 responses by regulating survival of the appropriate dendritic cell subset.  (+info)

Endothelial cells modulate the proliferation of mural cell precursors via platelet-derived growth factor-BB and heterotypic cell contact. (4/7795)

Embryological data suggest that endothelial cells (ECs) direct the recruitment and differentiation of mural cell precursors. We have developed in vitro coculture systems to model some of these events and have shown that ECs direct the migration of undifferentiated mesenchymal cells (10T1/2 cells) and induce their differentiation toward a smooth muscle cell/pericyte lineage. The present study was undertaken to investigate cell proliferation in these cocultures. ECs and 10T1/2 cells were cocultured in an underagarose assay in the absence of contact. There was a 2-fold increase in bromodeoxyuridine labeling of 10T1/2 cells in response to ECs, which was completely inhibited by the inclusion of neutralizing antiserum against platelet-derived growth factor (PDGF)-B. Antisera against PDGF-A, basic fibroblast growth factor, or transforming growth factor (TGF)-beta had no effect on EC-stimulated 10T1/2 cell proliferation. EC proliferation was not influenced by coculture with 10T1/2 cells in the absence of contact. The cells were then cocultured so that contact was permitted. Double labeling and fluorescence-activated cell sorter analysis revealed that ECs and 10T1/2 cells were growth-inhibited by 43% and 47%, respectively. Conditioned media from contacting EC-10T1/2 cell cocultures inhibited the growth of both cell types by 61% and 48%, respectively. Although we have previously shown a role for TGF-beta in coculture-induced mural cell differentiation, growth inhibition resulting from contacting cocultures or conditioned media was not suppressed by the presence of neutralizing antiserum against TGF-beta. Furthermore, the decreased proliferation of 10T1/2 cells in the direct cocultures could not be attributed to downregulation of the PDGF-B in ECs or the PDGF receptor-beta in the 10T1/2 cells. Our data suggest that modulation of proliferation occurs during EC recruitment of mesenchymal cells and that heterotypic cell-cell contact and soluble factors play a role in growth control during vessel assembly.  (+info)

Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis. (5/7795)

OBJECTIVE: To investigate the features of synovial stromal cells established from patients with rheumatoid arthritis (RA), and to define these cells as nurse cells. METHODS: Synovial nurse-like stromal cell lines (RA-SNCs) were established from patients with RA. These cell lines were examined for morphology, pseudoemperipolesis activity, cell surface markers, and cytokine production. The interaction between these RA-SNCs and a synovial tissue B cell clone was also examined. RESULTS: RA-SNCs had nurse cell activity. They spontaneously produced interleukin-6 (IL-6), IL-8, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. Furthermore, they produced IL-1beta and tumor necrosis factor alpha and expressed higher levels of the other cytokines after coculture with the B cell clone. Proliferation and Ig production by the B cell clone were dependent on direct contact with RA-SNCs. CONCLUSION: These results indicate that the RA-SNCs were nurse cells. The findings suggest that RA-SNCs may play an important role in the pathogenesis of RA by producing large amounts of cytokines and maintaining infiltrating lymphocytes.  (+info)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (6/7795)

Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease.  (+info)

In vitro hematopoietic and endothelial cell development from cells expressing TEK receptor in murine aorta-gonad-mesonephros region. (7/7795)

Recent studies have shown that long-term repopulating hematopoietic stem cells (HSCs) first appear in the aorta-gonad-mesonephros (AGM) region. Our immunohistochemistry study showed that TEK+ cells existed in the AGM region. Approximately 5% of AGM cells were TEK+, and most of these were CD34(+) and c-Kit+. We then established a coculture system of AGM cells using a stromal cell line, OP9, which is deficient in macrophage colony-stimulating factor (M-CSF). With this system, we showed that AGM cells at 10.5 days postcoitum (dpc) differentiated and proliferated into both hematopoietic and endothelial cells. Proliferating hematopoietic cells contained a significant number of colony-forming cells in culture (CFU-C) and in spleen (CFU-S). Among primary AGM cells at 10.5 dpc, sorted TEK+ AGM cells generated hematopoietic cells and platelet endothelial cell adhesion molecule (PECAM)-1(+) endothelial cells on the OP9 stromal layer, while TEK- cells did not. When a ligand for TEK, angiopoietin-1, was added to the single-cell culture of AGM, endothelial cell growth was detected in the wells where hematopoietic colonies grew. Although the incidence was still low (1/135), we showed that single TEK+ cells generated hematopoietic cells and endothelial cells simultaneously, using a single-cell deposition system. This in vitro coculture system shows that the TEK+ fraction of primary AGM cells is a candidate for hemangioblasts, which can differentiate into both hematopoietic cells and endothelial cells.  (+info)

Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8(+) T cells resulting in a failure to lyse tumor cells. (8/7795)

Dendritic cells (DC) are critically involved in the initiation of primary immune processes, including tumor rejection. In our study, we investigated the effect of interleukin-10 (IL-10)-treated human DC on the properties of CD8(+) T cells that are known to be essential for the destruction of tumor cells. We show that IL-10-pretreatment of DC not only reduces their allostimulatory capacity, but also induces a state of alloantigen-specific anergy in both primed and naive (CD45RA+) CD8(+) T cells. To investigate the influence of IL-10-treated DC on melanoma-associated antigen-specific T cells, we generated a tyrosinase-specific CD8(+) T-cell line by several rounds of stimulation with the specific antigen. After coculture with IL-10-treated DC, restimulation of the T-cell line with untreated, antigen-pulsed DC demonstrated peptide-specific anergy in the tyrosinase-specific T cells. Addition of IL-2 to the anergic T cells reversed the state of both alloantigen- or peptide-specific anergy. In contrast to optimally stimulated CD8(+) T cells, anergic tyrosinase-specific CD8(+) T cells, after coculture with peptide-pulsed IL-10-treated DC, failed to lyse an HLA-A2-positive and tyrosinase-expressing melanoma cell line. Thus, our data demonstrate that IL-10-treated DC induce an antigen-specific anergy in cytotoxic CD8(+) T cells, a process that might be a mechanism of tumors to inhibit immune surveillance by converting DC into tolerogenic antigen-presenting cells.  (+info)

... is a technique of assisted reproductive technology. It involves placing a patient's fertilized ... Coculture can be an effective treatment for patients who have failed previous IVF cycles or who have poor embryo quality. A ... A typical Coculture cycle consists of the following steps: 1. Once a patient has been deemed an appropriate candidate for the ... The risks of Coculture are minimal. The procedure has been performed in over 1000 patients with no reported detrimental effects ...
At the same clinic, he developed methods to treat male factor infertility using in vitro fertilization (IVF). He also co- ... He developed a precursor technique of Intracytoplasmic Sperm Injection (ICSI), which is now used for treatment of nearly all ... 1992). "Evaluation of Vero cell co-culture system for mouse embryos in various media". Human Reproduction. 7 (2): 276-80. doi: ... Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. Intracytoplasmic ...
... staining techniques and co-culturing using enrichment techniques, will allow proper cultivation and sequencing of TM7x cells. ... Using the medium enrichment technique can reduce the TM7x containing cultures from a complex community to a dual species co culture ... A stable co-culture of the TM7x and XH001 can be obtained by using a medium, which has been developed to resemble the saliva, ... The co-culture can be passed through a 28-gauge needle, after which it can be filtered using a 0.22 µm filter. ...
The technique of co-culturing is used to study cell crosstalk between two or more types of cells on a plate or in a 3D matrix. ... These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid ... This technique is known as two-dimensional (2D) cell culture, and was first developed by Wilhelm Roux who, in 1885, removed a ... Microfluidics technique is developed systems that can perform a process in a flow which are usually in a scale of micron. ...
Based on hypotheses about molecular regulators of valvular calcification and fibrosis, the lab uses in vitro co-culture systems ... It tries to identify though microgenomics techniques, the cellular and molecular differences in diseased vs. healthy heart ... Ongoing projects include utilization of novel biomaterials and measurement techniques, development of bioreactors, and ...
... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ... organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH E05.200.249.750 - tissue engineering MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ... patch-clamp techniques MeSH E05.200.750.132 - autoradiography MeSH E05.200.750.210 - bone demineralization technique MeSH ...
Immunological techniques are then commonly used to determine the species and/or serogroups of bacteria present in the sample.[ ... one study has shown that a coculture method that accounts for the close relationship with amoebae may be more sensitive, since ... New techniques for the rapid detection of Legionella in water samples have been developed, including the use of polymerase ... "Isolation of Legionella anisa using an amoebic coculture procedure". Journal of Clinical Microbiology. 39 (1): 365-6. doi: ...
Isolated cell culture, specifically co-culture of testis tissue, has been a useful technique for examining the influences of ... There are also techniques which are not practical or feasible in vivo which can now be explored. In vitro work is not without ... The development of this culture technique has taken place mainly with the use of animal models e.g. mice or rat testis tissue. ... Various categories of infertile men may benefit from advances in these techniques, especially those with a lack of viable ...
Bhatia also used co-cultures of more than one cell type to prevent dedifferentiation of the liver cells, building on the work ... She successfully applied techniques from photolithography to petri dishes, to create a substrate that would support growth of a ... She and her coworkers have also used techniques from 3D printing to create a lattice of sugar as a framework for a synthetic ...
In septo-hippocampal co-cultures, stimulation of cholinergic fibers reduced particular potassium currents, thus providing the ... To study nervous tissue under well-controlled experimental conditions, he established the organotypic slice culture technique, ... Novel three-dimensional organoid brain cultures in part build on methodologies developed with slice culture techniques. ... In the hypothalamus, his research focused on the characterization of interactions between co-cultured hypothalamic and ...
cocultures primary rat hepatocytes and 3T3-J2 fibroblasts in an 8*8 element array of microfluidic wells. Each well is separated ... In order to properly seed the 2D cell culture, a microcontact printing technique was used to lay out a fibronectin "brick wall ... This coculture method is extensively studied and is proved to be beneficial for extension of hepatocytes survival time and ... In order to co-culture these oxygen intolerant anaerobes with the oxygen favorable intestinal cells, a polysulfone fabricated ...
The techniques are now used by many pregnant women and prospective parents, especially couples who have a history of genetic ... is co-cultured with a serum containing necessary signals, or is grafted in a three-dimensional scaffold to result. Embryonic ... It is a common technique to use mouse cells and other animal cells to maintain the pluripotency of actively dividing stem cells ... Weiss, Rick (2007-12-07). "Scientists Cure Mice Of Sickle Cell Using Stem Cell Technique: New Approach Is From Skin, Not ...
... scaffold techniques and scaffold-free techniques. Scaffold techniques include the use of solid scaffolds, hydrogels and other ... Spheroids co-cultured with tumor and healthy cells were used to simulate how cancerous cells interact with normal cells. ... Scaffold free techniques employ another approach independent from the use scaffold. Scaffold-free methods include e.g. the use ... These techniques have been applied to in vitro disease models used to evaluate cellular responses to pharmaceutical compounds. ...
A variety of techniques has been developed for the isolation and culturing of amniotic stem cells. One of the more common ... Co-culturing, i.e. mixing cells and plating them together, of human amniotic stem cells with neonatal rat ventricular myocytes ... Mesenchymal stem cells (MSCs) are highly abundant in the amniotic fluid and several techniques have been described for their ... In general, these types of techniques are considered to be potentially significant but further investigations are required. ...
Genome-based analytical techniques may prove especially useful for L. anisa, as a study of isolates from various locations in ... La Scola B, Mezi L, Weiller PJ, Raoult D (2001). "Isolation of Legionella anisa Using an Amoebic Coculture Procedure". J Clin ... In addition, such techniques greatly reduce the time required to obtain results. Infections may be asymptomatic, and are ... However, some research suggests L. anisa may require a co-culture method that accounts for the close relationship with amoebae ...
He was a world authority on sex change operations, developing new techniques for transsexualism, embryo replacement, in vitro ... part of a team of clinicians and scientists which led to the world's first microinjection baby via human ampullary coculture in ... and how he used cadavers to come up with his technique of sexual reassignment surgery. It also shows his assistant, Dr Lim, ...
Follicles from different genetic sources can be co-cultured so that tissue origins can be differentiated within the co-culture ... This technique can also be used to study possible applications of fertility treatments e.g. isolating oocytes from primordial ... At this point the co-culture is frozen or fixed before further processing. To facilitate differentiation between tissue origins ... Morgan, Stephanie; Campbell, Lisa; Allison, Vivian; Murray, Alison; Spears, Norah (2015-03-17). "Culture and Co-Culture of ...
Recently, exometabolomics has been used to design co-culture systems. Because the analysis of extracellular metabolites allows ... Footprinting, in combination with other techniques, for early recognition of outbreak and strain characterization. Studying ... Exometabolomic techniques have been used in the following fields: Metabolite utilization to annotate function of unknown genes ... "Liquid chromatography-mass spectrometry for metabolic footprinting of co-cultures of lactic and propionic acid bacteria". ...
Other microscopy techniques are commonly used in tandem with NanoSIMS that allow for multiple types of information to be ... NanoSIMS can be used for pure cultures, co-cultures, and mixed community samples. The first use of NanoSIMS in biology was by ... This technique can be used to study nutrient exchange. The mouse gut microbiome was investigated to determine which microbes ... Unlike other imaging techniques, where 13C14N and 12C15N cannot be independently measured due to nearly identical masses, ...
Pre-implantation techniques include PGD, but also sperm sorting.[citation needed] Other assisted reproduction techniques ... Autologous endometrial coculture is a possible treatment for patients who have failed previous IVF attempts or who have poor ... The latter technique has proved to be less deleterious for the embryo, therefore it is advisable to perform the biopsy around ... This technique is used in cases when mothers carry genes for mitochondrial diseases. The therapy is approved for use in the ...
Co-culturing of different cell types can be achieved at the onset of levitation, by mixing different cell types in before ... Molecular biology techniques). ... Co-culturing 3T3-L1 pre-adipocytes in 3D with murine ... Co-culturing in a realistic tissue architecture is critical for accurately modeling in vivo conditions, such as for increasing ... One of the challenges in generating in vivo like cultures or tissue in vitro is the difficulty in co-culturing different cell ...
The technique can be applied to a large number of cells to produce stem cells for medical purposes on an industrial scale. ... While initial studies revealed that co-culturing epithelial cells with Swiss 3T3 cells J2 was essential for CRC induction, with ... The technique enables researchers to search large libraries of antibodies and quickly select the ones with a desired biological ... Three techniques are widely recognized: Transplantation of nuclei taken from somatic cells into an oocyte (egg cell) lacking ...
Clones of the S and L types could co-exist stably in co-culture with each other, indicating they occupied distinct niches in ... A later study by Leiby and Marx that used more advanced techniques showed that much of the decay Cooper and Lenski had ... and as technology and methodological techniques have advanced. The use of E. coli as the experimental organism has allowed many ... by Eric Quandt in the lab of Jeffrey Barrick at the University of Texas at Austin described the application of a new technique ...
In 2016, researchers developed a technique that used cancer cells' RNA to produce T cells and an immune response. They encased ... They then produce pure cultures of lymphocytes that can be tested for reactivity against other tumors, in coculture assays. ... As of 2015 the technique had expanded to treat cervical cancer, lymphoma, leukemia, bile duct cancer and neuroblastoma and in ... Cell culture techniques, Experimental cancer treatments, Immunotherapy). ...
This technique is also used in sheep, pigs and other animals. See In animals. Oocytes are classified depending on their ... Kannan S, Mehta A, Simha V, Reddy OK, Kaur BP, Onteru SK, Singh D (2014). "Photoinduction of granulosa cell and oocyte co-culture ... The best oocytes are chosen to be matured in the hope of then being implanted using in vitro fertilisation techniques. The ... IVM is not an entirely perfected technique. Pregnancy rates are lower in IVM than in standard IVF. There is also research ...
Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture ... This technique has demonstrated a stark difference in the sensitivity of the peripheral terminals compared to the neuronal cell ... After gelation, the agarose microgels will serve as a 3D microenvironment for cell co-culture. Segregated co-culture is also ... co-culture, and segregated (or indirect) co-culture. The use of conditioned media, where the cultured medium of one cell type ( ...
Apart from co-culturing intestinal cells with other cell types, also the cell population of the intestinal epithelium is of ... Apart from designing and fabricating the microfluidic device itself, microfabrication techniques are also used to create 3D ... First steps were taken in co-culturing the intestinal epithelium and the microbiota in intestine-on-a-chip systems. Examples ... Similarly, researchers have tried to recreate an immunocompetent intestinal epithelium in intestine-on-a-chip systems, by co- ...
Two major alternatives to in vivo animal testing are in vitro cell culture techniques and in silico computer simulation. ... circulatory flowrate and zonation and multi cellular co-cultures", the biochips have set themselves apart from basic cell ... One model is the "lung-on-a-chip". This combines microfabrication techniques with modern tissue engineering and mimics the ... 33 (439). Russell, W.M.S. and Burch, R.L., (1959). The Principles of Humane Experimental Technique, Methuen, London. ISBN 0- ...
Most significantly, the monolayers can be characterized with MALDI mass spectrometry in a technique known as SAMDI-MS, which ... Several strategies using this approach were studied in the context of cell signaling, migration, and co-culture. Subsequent ... Most recently, Mrksich's group has focused on developing a technique for assembling large molecular structures with perfectly ...
Optical detection includes fluorescence-based techniques, chemiluminescence-based techniques, and surface plasmon resonance ( ... Micropatterned co-cultures have also contributed to bio-MEMS for tissue engineering to recapitulate in vivo conditions and 3D ... One common use for this technique is in detecting nucleotide mismatches in DNA because the variation in mass caused by the ... Conventional micromachining techniques such as wet etching, dry etching, deep reactive ion etching, sputtering, anodic bonding ...
When a tissue carrier is turned, cells can also be seeded on the other side so that co-culture experiments with two different ... Molecular biology techniques). ...
The co-cultured species should not just be biofilters, but harvestable crops of commercial value. A working IMTA system should ... including the development of modern broiler rearing techniques, the adoption of the Cornish Cross broiler, the use of laying ... result in greater production for the overall system, based on mutual benefits to the co-cultured species and improved ecosystem ...
However, they hardly form blooms with sufficiently high concentration to be visible without enrichment techniques. Purple ... "Photoproduction of H2 from acetate by syntrophic cocultures of green sulfur bacteria and sulfur-reducing bacteria". Archives of ...
Co-cultures consisting of pericytes and endothelial cells induce the expression of TIMP-3 by pericytes, while endothelial cells ... Examination of cathepsin activity by using chemical probes and in vivo imaging techniques demonstrated an increase in cathepsin ...
These techniques have allowed for the introduction of new crop traits as well as a far greater control over a food's genetic ... microbes to produce precursors of the chemotherapeutic agent paclitaxel by applying the metabolic engineering in a co-culture ... For example, this technique was used with corn to produce the largest and sweetest crops. In the early twentieth century ... Compared with conventional breeding techniques, genetic engineering is far more precise and, in most cases, less likely to ...
This is one potential distinction from the age-old practice of aquatic polyculture, which could simply be the co-culture of ... "Aquaculture's growth continuing: improved management techniques can reduce environmental effects of the practice. (UPDATE)." ... A working IMTA system can result in greater total production based on mutual benefits to the co-cultured species and improved ... Onshore recirculating aquaculture systems, facilities using polyculture techniques, and properly sited facilities (for example ...
... culturing of anaerobes requires special techniques. A number of techniques are employed by microbiologists when culturing ... "Isolation and culture of Methanobrevibacter smithii by co-culture with hydrogen-producing bacteria on agar plates". Clinical ... or techniques such as injection of the bacteria into a dicot plant, which is an environment with limited oxygen. The GasPak ...
Thus, a combination of the flap and AVB techniques can maximize the growth rate and vascular contact of the bioreactor as ... These cells secrete growth factors that promote repair and can be co-cultured with stem cells to improve tissue formation. ... Service, Robert F. (29 July 2005). "Technique Uses Body as 'Bioreactor' to Grow New Bone". Science. 309 (5735): 683. doi: ... A Novel Technique for Periosteal Harvest". Journal of Investigative Surgery. 17 (4): 229-233. doi:10.1080/08941930490472073. ...
More recently, however, studies in 3D human coculture are proving to be an effective method with which to study cancer risks in ... Novel multicolor fluorescence painting techniques of human chromosomes have clearly demonstrated that high-LET α-particles and ...
Emerging optic-based techniques, for example, the pH optrode (or optode), which can be integrated into a self-referencing ... "Membrane potential depolarization causes alterations in neuron arrangement and connectivity in cocultures". Brain and Behavior ... These techniques allow further characterization of the wound electric fields/currents at cornea and skin wounds, which show ... The gold standard techniques to quantitatively extract electric dimensions from living specimens, ranging from cell to organism ...
This study demonstrated that both the monoculture extracts of the native strain T. maxima and its co-culture with P. carneus ... recovery of gold from electronic waste using mycofiltration techniques. Fungi are amongst the primary saprotrophic organisms in ... Chan-Cupul W, Heredia-Abarca G, Rodríguez-Vázquez R (2016). "Atrazine degradation by fungal co-culture enzyme extracts under ... a prospective environmental friendly technique of bioseparation and dewatering of domestic wastewater sludge". Environmental ...
Techniques for cell isolation depend on the cell source. Centrifugation and apheresis are techniques used for extracting cells ... Collagen/hyaluronic acid scaffolds have been used for modeling the mammary gland In Vitro while co-coculturing epithelial and ... This technique does not require the use of a solid porogen like SCPL. First, a synthetic polymer is dissolved into a suitable ... Each of these techniques presents its own advantages, but none are free of drawbacks. Molecular self-assembly is one of the few ...
Distinct Stages of Myelination Regulated by Y-Secretase and Astrocytes in a Rapidly Myelinating CNS Coculture System. 555-569 ... and mechanistic function of myelinogenesis has traditionally been studied using ultrastructure and biochemical techniques in ...
Different growth techniques have been developed. Different modes of growth (autotrophic, heterotrophic, and mixotrophic) has ... "Increased growth of the microalga Chlorella vulgaris when coimmobilized and cocultured in alginate beads with the plant-growth- ... differ depending on the technique used to create biomass and can be therefore targeted. Under more hostile conditions, the ...
... ACS Chem Neurosci. 2020 Sep 2 ... After 30 days in coculture, myelin segments were visualized using immunocytochemistry for myelin basic protein surrounding ... Coculture Techniques * Humans * Motor Neurons * Myelin Sheath* * Schwann Cells* Grant support * R01 NS050452/NS/NINDS NIH HHS/ ...
Coculture Techniques Actions. * Search in PubMed * Search in MeSH * Add to Search ... A similar ratio was induced in normal stromal cells cocultured with epithelial cells and exposed to TCDD. Disruption of stromal ... Reduced expression of progesterone receptor-B in the endometrium of women with endometriosis and in cocultures of endometrial ... Reduced expression of progesterone receptor-B in the endometrium of women with endometriosis and in cocultures of endometrial ...
Coculture Techniques Actions. * Search in PubMed * Search in MeSH * Add to Search ... TeloHAEC-GFPs and hTERT-MSCs were co-cultured for 7 days. The co-cultured cells showed a fluorescent branching structure, which ... Key aspects for conception and construction of co-culture models of tumor-stroma interactions. Mason J, Öhlund D. Mason J, et ... The co-culture model developed by using hTERT-immortalized cell lines described in this report provides a consistent and robust ...
... using that technique, Rowbotham was able to grow L. pneumophila from human feces (43). Moreover, the amoebal coculture is a ... Amoebal Coculture. By amoebal coculture, we recovered seven (1.6%) strains of ARB from nasal swabs of 4 of 444 participants. ... Amoebal Coculture. The microplates were centrifuged at 2,879 X g (4,000 rpm) for 30 minutes and incubated at 32°C in a ... The amoebal cocultures were subcultured on fresh amoebae on days 4 and 7 (samples from controls and patients) or on day 6 ( ...
Advanced training in cell culture and co-culturing techniques. *Immunohistochemical and immunofluorescence staining techniques ... Opportunities to learn RNA, DNA and protein assessment techniques. * ... as well as endocrine and neurotoxicology techniques. ...
Cell-culture-techniques; Cell-cultures; Lung-cells; Animals; Humans; Laboratory-animals; Author Keywords: Coculture; Gene ... As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer ... expressed in both cell types in coculture than in monoculture. When reduced to only those genes involved in inflammation and ... induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene ...
MeSH Terms: Animals; Cell Transformation, Neoplastic; Coculture Techniques; Mesenchymal Stromal Cells/physiology*; Mice; Mice, ... are able to confer tumor-promoting property to the naïve cocultured BM-MSCs. Examination of cytokines and chemokines showed ...
Short-Term Courses In Human Embryonic Stem Cell Culture Techniques PAR-05-133. NIH ... Use of coculture techniques to support maintenance of the undifferentiated cell state. ... polymerase chain reaction techniques, fluorescent in situ hybridization techniques and karyotype analysis (including staining ... Title: Short-Term Courses In Human Embryonic Stem Cell Culture Techniques. Announcement Type This is a reissue of PA-02-054, ...
Using large-scale acid-extraction techniques, we demonstrate a step-by-step process to isolate collagen I from commercially ... In line with emerging 3D in vitro techniques, such as organoid cultures, as pre-clinical testing grounds, there is a need for ... The organotypic co-culture platform was originally developed as an artificial skin model for the assessment of SCC invasion in ... Organotypic co-cultures bridge the gap between standard two-dimensional culture and mouse models. Such assays increase the ...
6. A Co-Culture-Based Multiparametric Imaging Technique to Dissect Local H. Secilmis M; Altun HY; Pilic J; Erdogan YC; Cokluk Z ...
Dive into the research topics of Phosphoinositide 3-Kinase Alpha-Dependent Regulation of Branching Morphogenesis in Murine Embryonic Lung: Evidence for a Role in Determining Morphogenic Properties of FGF7. Together they form a unique fingerprint. ...
These IWCA co-culture TEER values are similar to those previously reported by others at 5 days from co-cultures of bEnd.3 cells ... Srinivasan, B., Kolli, A. R., Esch, M. B., Abaci, H. E., Shuler, M. L., and Hickman, J. J. (2015). TEER measurement techniques ... Whether co-culture of immortalized astrocytes with neurons and brain endothelial cells can induce GLT-1 expression in hTERT ... Companion inserts without IWCA were seeded with bEnd.3 alone to compare their barrier function to that of the co-cultures. ...
Coculture Techniques Preferred Concept UI. M0028289. Scope Note. A technique of culturing mixed cell types in vitro to allow ... Coculture can be of different types of cells, tissues, or organs from normal or disease states.. Terms. Coculture Techniques ... Investigative Techniques [E05] * In Vitro Techniques [E05.481] * Culture Techniques [E05.481.500] * Axenic Culture [E05.481. ... Coculture can be of different types of cells, tissues, or organs from normal or disease states.. Entry Term(s). Co-culture ...
Expression of TSG-6 mRNA in MCs cocultured with DFAT cells was significantly higher than in mesangial cells or DFAT cells alone ... A) Transwell coculture systems of DFAT cells and MCs. MC: MCs alone. DFAT: DFAT cells alone. MC + DFAT: MCs were seeded in the ... Glomeruli were isolated with a graded-sieve technique as described previously [19]. Mesangial cells (MCs) from SHR-SP were ... Coculture of DFAT and mesangial cells. Glomeruli were isolated from kidneys of four-week-old male stroke prone spontaneous ...
... and cutting-edge techniques of co-culture and 3D printed structures in parallel with large scale Omics studies including single ...
We co-culture human brain microvascular EC and human astrocytes on a -- on a tissue culture insert that as three micron pores. ... And well use the exquisite techniques to assess HIV, RNA, and DNA in these cells. Additionally, in Aim 2, we will have an in ... We put cell pre-virus, Luciferase virus, which often is less effective, you know, on the -- and put it on the top of the co-culture ... And lastly, we developed our growth assay that a co-culture of the microglial cell with CEM174 T cell, and we found that the ...
Coculture Techniques Medicine & Life Sciences 66% * Nanoparticles Engineering & Materials Science 65% * Cells Engineering & ... were preferentially internalized by breast cancer cells in co-cultures with osteoblasts. While osteoblasts partially ... were preferentially internalized by breast cancer cells in co-cultures with osteoblasts. While osteoblasts partially ... were preferentially internalized by breast cancer cells in co-cultures with osteoblasts. While osteoblasts partially ...
Coculture Techniques 9% * Brain 8% * Electric Stimulation 8% * Action Potentials 8% * Neuroglia 8% ...
The aim of this proposal is to develop a co-culture system of RPE and endothelial cells on a biodegradable, biocompatible poly( ... However, current techniques of gene expression control (gene deletion, overexpression, knockdown, etc.) are aimed to control ... The development of tissue culture techniques by Ross Granville Harrison in 1907 has been cited as one of the ten greatest ... My overarching goal is to use new molecular techniques to understand placental function and its relationship to fetal outcomes ...
The project will involve the techniques below, in which the student will receive training: - Development and use of co-culture ... This will involve molecular biology techniques including PCR and gene sequencing/annotation. Megaw, J., Kelly, S. A., Thompson ... using field techniques and skills such as wildlife tracking, taxonomy, geostatistics, molecular and genetic ecology, ... learning to use a piece of apparatus or technique) to generic (e.g. developing oral presentation or leadership skills). Every ...
Our group uses a range of techniques from cell biology, molecular biology, omics, co-culture and in vivo models, transgenic and ... To this end, we study the impact of modulating signaling pathways in cultured cells and rodents using cutting-edge techniques ... Quantitative phosphoproteomic analysis by mass spectrometry is a technique that allows efficient profiling of tens of thousands ... To visualize signal transduction events, we design and generate novel fluorescent probes using molecular biology techniques, ...
The project will use cutting edge technologies, including ex vivo 3D organoid co-culture models, to study interactions between ... Our collaborative team has expertise in a wide variety of interdisciplinary techniques to facilitate the proposed PhD research ... as well as a broad range of molecular cell biology techniques, to address the crosstalk between p53 and inflammation. The ...
Equipment, space and materials/reagents for standard and specialized 3D cell culture (mono and cocultures, organs-on-a-chip) - ... Techniques Available via Partnership and Collaboration at BNC. * Molding and laser-shaping for organs-on-chips. ...
Virus isolation by co-culturing techniques confirmed productive infection of both cell types. Only in animals exhibiting SIVE/ ...
Coculture Techniques * Concanavalin A * Dietary Fats * Docosahexaenoic Acids * Fatty Acids, Omega-3 ... Suppression of T cell proliferation in the co-cultures was dependent upon the dose of dietary n-3 PUFA fed to mice from which ... Purified T cells or accessory cells from each diet group were co-cultured with the alternative cell type from every other diet ... Dietary n-3 PUFA affect TcR-mediated activation of purified murine T cells and accessory cell function in co-cultures. Academic ...
Advanced training in cell culture and co-culturing techniques. *Immunohistochemical and immunofluorescence staining techniques ... Opportunities to learn RNA, DNA and protein assessment techniques. * ... as well as endocrine and neurotoxicology techniques. ...
Coculture Techniques Entry term(s). Co culture Co-culture Co-cultures Cocultivation Cocultivations Coculture Coculture ... Co culture. Co-culture. Co-cultures. Cocultivation. Cocultivations. Coculture. Coculture Technique. Cocultures. Tree number(s): ... Coculture Techniques - Preferred Concept UI. M0028289. Scope note. A technique of culturing mixed cell types in vitro to allow ... A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL ...
C16.131.666.507.450.499.249 Coculture Techniques E5.240.374 E5.481.500.374 Colloids D27.720.280.165 Colonoscopy E4.800.250.250. ... I1.880.853.100 Culture Techniques E5.240 E5.481.500 Cystoscopy E4.800.250.180 E4.502.250.180 Cytochrome P-450 CYP1A1 D8.244. ... A8.713.49.875 Plant Somatic Embryogenesis Techniques E5.240.550 E5.481.500.550 Plum Pox Virus B4.820.464.600.600 Post-Exposure ... A11.671.501.145 Batch Cell Culture Techniques E5.240.249.124 E5.481.500.249.124 Battered Child Syndrome I1.880.787.293.292.304 ...
Here, a fabrication technique (gravitational lumen patterning or GLP) is developed to create a lymphangion-chip. This organ-on- ... This system successfully characterizes the dynamics of cell size, density, growth, alignment, and intercellular gap due to co-culture ... Lymphangion-chip: a microphysiological system which supports co-culture and bidirectional signaling of lymphatic endothelial ... chip consists of co-culture of a monolayer of endothelial lumen surrounded by multiple and uniformly thick layers of muscle ...
  • A similar ratio was induced in normal stromal cells cocultured with epithelial cells and exposed to TCDD. (nih.gov)
  • This study compared global multi-walled carbon nanotube (MWCNT)-induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene expression from mouse lungs exposed to MWCNT. (cdc.gov)
  • Construction and optimization of a coculture system of mouse brain microvascular endothelial cells and myelin debris. (bvsalud.org)
  • Several methods for the preparation of myelin debris and parameters for constructing a coculture system of microvascular endothelial cells and myelin debris are available, but no systematic studies have yet been conducted, which hinders further exploration of the mechanisms of demyelinating disease repair. (bvsalud.org)
  • Microvascular endothelial cells were cultured on a matrix gel, and myelin debris of different sizes (fluorescently labeled using CFSE) was placed in coculture after forming a vascular-like structure. (bvsalud.org)
  • Subsequently, myelin debris of different concentrations was cocultured in the vascular-like structure, and phagocytosis of myelin debris by microvascular endothelial cells was detected using immunofluorescence staining and flow cytometry . (bvsalud.org)
  • We found that myelin debris could be successfuly obtained from the mouse brain with secondary grinding and other steps and cocultured with microvascular endothelial cells at a concentration of 2 mg/mL, which promoted the phagocytosis of microvascular endothelial cells . (bvsalud.org)
  • In conclusion, we provide a reference for the protocol of a coculture system of microvascular endothelial cells and myelin debris. (bvsalud.org)
  • The present study describes the presence of u-PA in supernatants of pure cultures of human glomerular epithelial cells (EC), cocultures of EC and human mesangial cells (MC) and whole glomeruli, but not within pure cultures of MC. (elsevier.com)
  • Expression of TSG-6 mRNA in MCs cocultured with DFAT cells was significantly higher than in mesangial cells or DFAT cells alone. (biomedcentral.com)
  • To confirm the synthesis of u-PA mRNA in glomerular EC, cocultures of EC and MC were tested by in situ hybridization with u-PA antisense and sense digoxigenin-labeled RNA probes. (elsevier.com)
  • After 30 days in coculture, myelin segments were visualized using immunocytochemistry for myelin basic protein surrounding neurofilament-stained motor neuron axons, which was confirmed via 3D confocal Raman microscopy, a viable alternative for transmission electron microscopy analysis. (nih.gov)
  • Coculture can be of different types of cells, tissues, or organs from normal or disease states. (nih.gov)
  • Coculturing HT29 human colon carcinoma cells with human platelets led to the induction of mesenchymal-like cancer cells characterized by downregulation of E-cadherin and upregulation of Twist1, enhanced cell mobility and a proaggregatory action on platelets. (oncotarget.com)
  • Investigations using these techniques revealed that coculture of Kupffer cells and hepatoma cells stimulate productions of NO and TNF-alpha, and these mediators released from Kupffer cells synergistically induce apoptosis of hepatoma cells. (nii.ac.jp)
  • Inhibitor studies using antisense and sense oligodeoxynucleotides against mRNAs of iNOS and TNF-alpha, antibodies against adhesion molecules suggested that interactions via CD18 and ICAM-1 leads to activation and mediator production of Kupffer cells cocultured with hepatoma cells. (nii.ac.jp)
  • Invest in the development of high-throughput systems of AD-relevant cells and organoids driven by robotics with digital read-outs (such as high content imaging) to leverage reinforcement learning techniques for more data-driven target discovery/screening. (nih.gov)
  • We tried to recover ARB and free-living amoebae by using amoebal coculture and amoebal enrichment, respectively, with any recovered amoebae subsequently examined for the presence of ARB. (cdc.gov)
  • Using a cutoff of 10% false discovery rate and 1.5 fold change, we determined that there were more concordant genes (gene expression both up- or downregulated in vivo and in vitro) expressed in both cell types in coculture than in monoculture. (cdc.gov)
  • When reduced to only those genes involved in inflammation and fibrosis, known outcomes of in vivo MWCNT exposure, there were more disease-related concordant genes expressed in coculture than monoculture. (cdc.gov)
  • As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer enhanced in vitro models for nanoparticle risk assessment and the reduction of in vivo toxicological testing. (cdc.gov)
  • Despite the revolutionary impact these phenomena have had on neuroscience, microscopy, and lithography, it has been generally very difficult to apply this technique in vivo to stimulate biomaterials, diagnostics, and drug delivery systems. (nih.gov)
  • This project allows me to combine my interests in visualization techniques and cellular biology to better understand the key molecular mechanisms responsible for brain metastasis development. (braintumour.ca)
  • This will involve molecular biology techniques including PCR and gene sequencing/annotation. (qub.ac.uk)
  • To isolate new ARB, we performed amoebal coculture from 444 nasal samples. (cdc.gov)
  • Using large-scale acid-extraction techniques, we demonstrate a step-by-step process to isolate collagen I from commercially available animal byproducts. (nature.com)
  • Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human pathogens. (cdc.gov)
  • Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB) and clean the samples of other more rapidly growing species that generally overwhelm the agar plates. (cdc.gov)
  • This will also involve assessment of the antimicrobial susceptibility of archaea and bacteria and other standard culturing techniques. (qub.ac.uk)
  • Laboratory of New Techniques of Restoration & Reconstruction, Institute of Traumatology & Orthopedics, Nanjing University of Chinese Medicine, Nanjing, China. (bvsalud.org)
  • A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS . (nih.gov)
  • Virus isolation by co-culturing techniques confirmed productive infection of both cell types. (nih.gov)
  • The National Institutes of Health (NIH) invites applications for grants to develop and conduct short-term continuing education programs on laboratory research techniques for human embryonic stem cell (hESC) lines, and to disseminate course materials and instructional experience to the scientific community. (nih.gov)
  • In this study, using a mouse lymphoma model, we showed that the lymphoma resident MSCs (L-MSCs) are able to confer tumor-promoting property to the naïve cocultured BM-MSCs. (nih.gov)
  • This barrier is gradually being overcome with genomic techniques in which animal models, such as the mouse, can be sapienized. (nih.gov)
  • This approach is complemented with the use of a wide range of cell culture lines (standard cell lines, patient-derived primary and iPSCs) and cutting-edge techniques of co-culture and 3D printed structures in parallel with large scale Omics studies including single cell RNA sequencing, proteomics and metabolomics. (nih.gov)
  • A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS . (nih.gov)
  • Thus, amoebal coculture allows the recovery of new bacterial species from heavily contaminated samples and might be a valuable approach for the recovery of as-yet unrecognized emerging pathogens from clinical specimens. (cdc.gov)
  • Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human pathogens. (cdc.gov)
  • The National Institutes of Health (NIH) invites applications for grants to develop and conduct short-term continuing education programs on laboratory research techniques for human embryonic stem cell (hESC) lines, and to disseminate course materials and instructional experience to the scientific community. (nih.gov)
  • This approach is complemented with the use of a wide range of cell culture lines (standard cell lines, patient-derived primary and iPSCs) and cutting-edge techniques of co-culture and 3D printed structures in parallel with large scale Omics studies including single cell RNA sequencing, proteomics and metabolomics. (nih.gov)