Coculture techniques. Medical search

A technique of culturing mixed cell types in vitro to allow their synergistic or antagonistic interactions, such as on CELL DIFFERENTIATION or APOPTOSIS. Coculture can be of different types of cells, tissues, or organs from normal or disease states.

Any of several ways in which living cells of an organism communicate with one another, whether by direct contact between cells or by means of chemical signals carried by neurotransmitter substances, hormones, and cyclic AMP.

Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.

Connective tissue cells of an organ found in the loose connective tissue. These are most often associated with the uterine mucosa and the ovary as well as the hematopoietic system and elsewhere.

Culture media containing biologically active components obtained from previously cultured cells or tissues that have released into the media substances affecting certain cell functions (e.g., growth, lysis).

Cellular signaling in which a factor secreted by a cell affects other cells in the local environment. This term is often used to denote the action of INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS on surrounding cells.

Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.

Connective tissue cells which secrete an extracellular matrix rich in collagen and other macromolecules.

Methods for maintaining or growing CELLS in vitro.

Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.

Benzoic acid or benzoic acid esters substituted with one or more bromine atoms.

Cells contained in the bone marrow including fat cells (see ADIPOCYTES); STROMAL CELLS; MEGAKARYOCYTES; and the immediate precursors of most blood cells.

Established cell cultures that have the potential to propagate indefinitely.

Technique for treating DEHYDRATION and WATER-ELECTROLYTE IMBALANCE by subcutaneous infusion of REHYDRATION SOLUTIONS.

Single pavement layer of cells which line the luminal surface of the entire vascular system and regulate the transport of macromolecules and blood components.

Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.

Generating tissue in vitro for clinical applications, such as replacing wounded tissues or impaired organs. The use of TISSUE SCAFFOLDING enables the generation of complex multi-layered tissues and tissue structures.

Large, phagocytic mononuclear leukocytes produced in the vertebrate BONE MARROW and released into the BLOOD; contain a large, oval or somewhat indented nucleus surrounded by voluminous cytoplasm and numerous organelles.

Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.

Lymphocytes responsible for cell-mediated immunity. Two types have been identified - cytotoxic (T-LYMPHOCYTES, CYTOTOXIC) and helper T-lymphocytes (T-LYMPHOCYTES, HELPER-INDUCER). They are formed when lymphocytes circulate through the THYMUS GLAND and differentiate to thymocytes. When exposed to an antigen, they divide rapidly and produce large numbers of new T cells sensitized to that antigen.

Bone-marrow-derived, non-hematopoietic cells that support HEMATOPOETIC STEM CELLS. They have also been isolated from other organs and tissues such as UMBILICAL CORD BLOOD, umbilical vein subendothelium, and WHARTON JELLY. These cells are considered to be a source of multipotent stem cells because they include subpopulations of mesenchymal stem cells.

Specialized cells of the hematopoietic system that have branch-like extensions. They are found throughout the lymphatic system, and in non-lymphoid tissues such as SKIN and the epithelia of the intestinal, respiratory, and reproductive tracts. They trap and process ANTIGENS, and present them to T-CELLS, thereby stimulating CELL-MEDIATED IMMUNITY. They are different from the non-hematopoietic FOLLICULAR DENDRITIC CELLS, which have a similar morphology and immune system function, but with respect to humoral immunity (ANTIBODY PRODUCTION).

All of the processes involved in increasing CELL NUMBER including CELL DIVISION.

Morphologic alteration of small B LYMPHOCYTES or T LYMPHOCYTES in culture into large blast-like cells able to synthesize DNA and RNA and to divide mitotically. It is induced by INTERLEUKINS; MITOGENS such as PHYTOHEMAGGLUTININS, and by specific ANTIGENS. It may also occur in vivo as in GRAFT REJECTION.

RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.

Non-antibody proteins secreted by inflammatory leukocytes and some non-leukocytic cells, that act as intercellular mediators. They differ from classical hormones in that they are produced by a number of tissue or cell types rather than by specialized glands. They generally act locally in a paracrine or autocrine rather than endocrine manner.

Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.

The fission of a CELL. It includes CYTOKINESIS, when the CYTOPLASM of a cell is divided, and CELL NUCLEUS DIVISION.

A genus of free-living soil amoebae that produces no flagellate stage. Its organisms are pathogens for several infections in humans and have been found in the eye, bone, brain, and respiratory tract.

A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.

The relatively long-lived phagocytic cell of mammalian tissues that are derived from blood MONOCYTES. Main types are PERITONEAL MACROPHAGES; ALVEOLAR MACROPHAGES; HISTIOCYTES; KUPFFER CELLS of the liver; and OSTEOCLASTS. They may further differentiate within chronic inflammatory lesions to EPITHELIOID CELLS or may fuse to form FOREIGN BODY GIANT CELLS or LANGHANS GIANT CELLS. (from The Dictionary of Cell Biology, Lackie and Dow, 3rd ed.)

A pair of highly specialized muscular canals extending from the UTERUS to its corresponding OVARY. They provide the means for OVUM collection, and the site for the final maturation of gametes and FERTILIZATION. The fallopian tube consists of an interstitium, an isthmus, an ampulla, an infundibulum, and fimbriae. Its wall consists of three histologic layers: serous, muscular, and an internal mucosal layer lined with both ciliated and secretory cells.

The span of viability of a cell characterized by the capacity to perform certain functions such as metabolism, growth, reproduction, some form of responsiveness, and adaptability.

Adherence of cells to surfaces or to other cells.

The intracellular transfer of information (biological activation/inhibition) through a signal pathway. In each signal transduction system, an activation/inhibition signal from a biologically active molecule (hormone, neurotransmitter) is mediated via the coupling of a receptor/enzyme to a second messenger system or to an ion channel. Signal transduction plays an important role in activating cellular functions, cell differentiation, and cell proliferation. Examples of signal transduction systems are the GAMMA-AMINOBUTYRIC ACID-postsynaptic receptor-calcium ion channel system, the receptor-mediated T-cell activation pathway, and the receptor-mediated activation of phospholipases. Those coupled to membrane depolarization or intracellular release of calcium include the receptor-mediated activation of cytotoxic functions in granulocytes and the synaptic potentiation of protein kinase activation. Some signal transduction pathways may be part of larger signal transduction pathways; for example, protein kinase activation is part of the platelet activation signal pathway.

Progenitor cells from which all blood cells derive.

A cell line derived from cultured tumor cells.

Relatively undifferentiated cells that retain the ability to divide and proliferate throughout postnatal life to provide progenitor cells that can differentiate into specialized cells.

Differentiation antigens residing on mammalian leukocytes. CD stands for cluster of differentiation, which refers to groups of monoclonal antibodies that show similar reactivity with certain subpopulations of antigens of a particular lineage or differentiation stage. The subpopulations of antigens are also known by the same CD designation.

A cytokine that stimulates the growth and differentiation of B-LYMPHOCYTES and is also a growth factor for HYBRIDOMAS and plasmacytomas. It is produced by many different cells including T-LYMPHOCYTES; MONOCYTES; and FIBROBLASTS.

One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.

A family of anaerobic, coccoid to rod-shaped METHANOBACTERIALES. Cell membranes are composed mainly of polyisoprenoid hydrocarbons ether-linked to glycerol. Its organisms are found in anaerobic habitats throughout nature.

The movement of cells from one location to another. Distinguish from CYTOKINESIS which is the process of dividing the CYTOPLASM of a cell.

Serum glycoprotein produced by activated MACROPHAGES and other mammalian MONONUCLEAR LEUKOCYTES. It has necrotizing activity against tumor cell lines and increases ability to reject tumor transplants. Also known as TNF-alpha, it is only 30% homologous to TNF-beta (LYMPHOTOXIN), but they share TNF RECEPTORS.

Penetrating and non-penetrating injuries to the spinal cord resulting from traumatic external forces (e.g., WOUNDS, GUNSHOT; WHIPLASH INJURIES; etc.).

A cylindrical column of tissue that lies within the vertebral canal. It is composed of WHITE MATTER and GRAY MATTER.

A surgical specialty concerned with the treatment of diseases and disorders of the brain, spinal cord, and peripheral and sympathetic nervous system.

Renewal or physiological repair of damaged nerve tissue.

A subspecialty of internal medicine concerned with morphology, physiology, and pathology of the blood and blood-forming tissues.

The long large endothelium-lined venous channel on the top outer surface of the brain. It receives blood from a vein in the nasal cavity, runs backwards, and gradually increases in size as blood drains from veins of the brain and the DURA MATER. Near the lower back of the CRANIUM, the superior sagittal sinus deviates to one side (usually the right) and continues on as one of the TRANSVERSE SINUSES.

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (1/7795)

Interleukin-12 (IL-12) is a potent antitumor cytokine, which induces and enhances the activity of natural killer (NK) cells, lymphokine activated killer (LAK) cells and cytotoxic T lymphocytes (CTL). IL-12 also stimulates IFN-gamma production from both T cells and NK cells. In this study, we transfected methylcholanthrene-induced fibrosarcoma (MCA-D) with TNF gene and investigated the therapeutic effect of TNF gene-transduced cancer vaccine and whether the vaccination effect is enhanced by systemic administration of recombinant IL-12 (rIL-12), in a murine model. TNF gene-transduced cancer vaccine or systemic administration of rIL-12 showed slight or moderate inhibition of pre-established tumor. However, simultaneous application of the vaccine and rIL-12 resulted in complete eradication. The cytotoxicity of CTL against parental tumor cells was enhanced with the combination of the vaccine and rIL-12, and IFN-gamma production from spleen cells also increased synergistically. Our findings show that synergistic enhancement of CTL activity and IFN-gamma production could play an important role in the antitumor effect of combination therapy using TNF gene-transduced cancer vaccine and rIL-12. (+info)

Regulation of neurotrophin-3 expression by epithelial-mesenchymal interactions: the role of Wnt factors. (2/7795)

Neurotrophins regulate survival, axonal growth, and target innervation of sensory and other neurons. Neurotrophin-3 (NT-3) is expressed specifically in cells adjacent to extending axons of dorsal root ganglia neurons, and its absence results in loss of most of these neurons before their axons reach their targets. However, axons are not required for NT-3 expression in limbs; instead, local signals from ectoderm induce NT-3 expression in adjacent mesenchyme. Wnt factors expressed in limb ectoderm induce NT-3 in the underlying mesenchyme. Thus, epithelial-mesenchymal interactions mediated by Wnt factors control NT-3 expression and may regulate axonal growth and guidance. (+info)

Reciprocal control of T helper cell and dendritic cell differentiation. (3/7795)

It is not known whether subsets of dendritic cells provide different cytokine microenvironments that determine the differentiation of either type-1 T helper (TH1) or TH2 cells. Human monocyte (pDC1)-derived dendritic cells (DC1) were found to induce TH1 differentiation, whereas dendritic cells (DC2) derived from CD4+CD3-CD11c- plasmacytoid cells (pDC2) induced TH2 differentiation by use of a mechanism unaffected by interleukin-4 (IL-4) or IL-12. The TH2 cytokine IL-4 enhanced DC1 maturation and killed pDC2, an effect potentiated by IL-10 but blocked by CD40 ligand and interferon-gamma. Thus, a negative feedback loop from the mature T helper cells may selectively inhibit prolonged TH1 or TH2 responses by regulating survival of the appropriate dendritic cell subset. (+info)

Endothelial cells modulate the proliferation of mural cell precursors via platelet-derived growth factor-BB and heterotypic cell contact. (4/7795)

Embryological data suggest that endothelial cells (ECs) direct the recruitment and differentiation of mural cell precursors. We have developed in vitro coculture systems to model some of these events and have shown that ECs direct the migration of undifferentiated mesenchymal cells (10T1/2 cells) and induce their differentiation toward a smooth muscle cell/pericyte lineage. The present study was undertaken to investigate cell proliferation in these cocultures. ECs and 10T1/2 cells were cocultured in an underagarose assay in the absence of contact. There was a 2-fold increase in bromodeoxyuridine labeling of 10T1/2 cells in response to ECs, which was completely inhibited by the inclusion of neutralizing antiserum against platelet-derived growth factor (PDGF)-B. Antisera against PDGF-A, basic fibroblast growth factor, or transforming growth factor (TGF)-beta had no effect on EC-stimulated 10T1/2 cell proliferation. EC proliferation was not influenced by coculture with 10T1/2 cells in the absence of contact. The cells were then cocultured so that contact was permitted. Double labeling and fluorescence-activated cell sorter analysis revealed that ECs and 10T1/2 cells were growth-inhibited by 43% and 47%, respectively. Conditioned media from contacting EC-10T1/2 cell cocultures inhibited the growth of both cell types by 61% and 48%, respectively. Although we have previously shown a role for TGF-beta in coculture-induced mural cell differentiation, growth inhibition resulting from contacting cocultures or conditioned media was not suppressed by the presence of neutralizing antiserum against TGF-beta. Furthermore, the decreased proliferation of 10T1/2 cells in the direct cocultures could not be attributed to downregulation of the PDGF-B in ECs or the PDGF receptor-beta in the 10T1/2 cells. Our data suggest that modulation of proliferation occurs during EC recruitment of mesenchymal cells and that heterotypic cell-cell contact and soluble factors play a role in growth control during vessel assembly. (+info)

Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis. (5/7795)

OBJECTIVE: To investigate the features of synovial stromal cells established from patients with rheumatoid arthritis (RA), and to define these cells as nurse cells. METHODS: Synovial nurse-like stromal cell lines (RA-SNCs) were established from patients with RA. These cell lines were examined for morphology, pseudoemperipolesis activity, cell surface markers, and cytokine production. The interaction between these RA-SNCs and a synovial tissue B cell clone was also examined. RESULTS: RA-SNCs had nurse cell activity. They spontaneously produced interleukin-6 (IL-6), IL-8, granulocyte colony-stimulating factor, and granulocyte-macrophage colony-stimulating factor. Furthermore, they produced IL-1beta and tumor necrosis factor alpha and expressed higher levels of the other cytokines after coculture with the B cell clone. Proliferation and Ig production by the B cell clone were dependent on direct contact with RA-SNCs. CONCLUSION: These results indicate that the RA-SNCs were nurse cells. The findings suggest that RA-SNCs may play an important role in the pathogenesis of RA by producing large amounts of cytokines and maintaining infiltrating lymphocytes. (+info)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (6/7795)

Understanding the pathogenesis of Alzheimer's disease is of widespread interest because it is an increasingly prevalent disorder that is progressive, fatal, and currently untreatable. The dementia of Alzheimer's disease is caused by neuronal cell death. We demonstrate for the first time that blood vessels isolated from the brains of Alzheimer's disease patients can directly kill neurons in vitro. Either direct co-culture of Alzheimer's disease microvessels with neurons or incubation of cultured neurons with conditioned medium from microvessels results in neuronal cell death. In contrast, vessels from elderly nondemented donors are significantly (P<0.001) less lethal and brain vessels from younger donors are not neurotoxic. Neuronal killing by either direct co-culture with Alzheimer's disease microvessels or conditioned medium is dose- and time-dependent. Neuronal death can occur by either apoptotic or necrotic mechanisms. The microvessel factor is neurospecific, killing primary cortical neurons, cerebellar granule neurons, and differentiated PC-12 cells, but not non-neuronal cell types or undifferentiated PC-12 cells. Appearance of the neurotoxic factor is decreased by blocking microvessel protein synthesis with cycloheximide. The neurotoxic factor is soluble and likely a protein, because its activity is heat labile and trypsin sensitive. These findings implicate a novel mechanism of vascular-mediated neuronal cell death in Alzheimer's disease. (+info)

In vitro hematopoietic and endothelial cell development from cells expressing TEK receptor in murine aorta-gonad-mesonephros region. (7/7795)

Recent studies have shown that long-term repopulating hematopoietic stem cells (HSCs) first appear in the aorta-gonad-mesonephros (AGM) region. Our immunohistochemistry study showed that TEK+ cells existed in the AGM region. Approximately 5% of AGM cells were TEK+, and most of these were CD34(+) and c-Kit+. We then established a coculture system of AGM cells using a stromal cell line, OP9, which is deficient in macrophage colony-stimulating factor (M-CSF). With this system, we showed that AGM cells at 10.5 days postcoitum (dpc) differentiated and proliferated into both hematopoietic and endothelial cells. Proliferating hematopoietic cells contained a significant number of colony-forming cells in culture (CFU-C) and in spleen (CFU-S). Among primary AGM cells at 10.5 dpc, sorted TEK+ AGM cells generated hematopoietic cells and platelet endothelial cell adhesion molecule (PECAM)-1(+) endothelial cells on the OP9 stromal layer, while TEK- cells did not. When a ligand for TEK, angiopoietin-1, was added to the single-cell culture of AGM, endothelial cell growth was detected in the wells where hematopoietic colonies grew. Although the incidence was still low (1/135), we showed that single TEK+ cells generated hematopoietic cells and endothelial cells simultaneously, using a single-cell deposition system. This in vitro coculture system shows that the TEK+ fraction of primary AGM cells is a candidate for hemangioblasts, which can differentiate into both hematopoietic cells and endothelial cells. (+info)

Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8(+) T cells resulting in a failure to lyse tumor cells. (8/7795)

Dendritic cells (DC) are critically involved in the initiation of primary immune processes, including tumor rejection. In our study, we investigated the effect of interleukin-10 (IL-10)-treated human DC on the properties of CD8(+) T cells that are known to be essential for the destruction of tumor cells. We show that IL-10-pretreatment of DC not only reduces their allostimulatory capacity, but also induces a state of alloantigen-specific anergy in both primed and naive (CD45RA+) CD8(+) T cells. To investigate the influence of IL-10-treated DC on melanoma-associated antigen-specific T cells, we generated a tyrosinase-specific CD8(+) T-cell line by several rounds of stimulation with the specific antigen. After coculture with IL-10-treated DC, restimulation of the T-cell line with untreated, antigen-pulsed DC demonstrated peptide-specific anergy in the tyrosinase-specific T cells. Addition of IL-2 to the anergic T cells reversed the state of both alloantigen- or peptide-specific anergy. In contrast to optimally stimulated CD8(+) T cells, anergic tyrosinase-specific CD8(+) T cells, after coculture with peptide-pulsed IL-10-treated DC, failed to lyse an HLA-A2-positive and tyrosinase-expressing melanoma cell line. Thus, our data demonstrate that IL-10-treated DC induce an antigen-specific anergy in cytotoxic CD8(+) T cells, a process that might be a mechanism of tumors to inhibit immune surveillance by converting DC into tolerogenic antigen-presenting cells. (+info)

At the same clinic, he developed methods to treat male factor infertility using in vitro fertilization (IVF). He also co- ... He developed a precursor technique of Intracytoplasmic Sperm Injection (ICSI), which is now used for treatment of nearly all ... 1992). "Evaluation of Vero cell co-culture system for mouse embryos in various media". Human Reproduction. 7 (2): 276-80. doi: ... Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. Intracytoplasmic ...

https://en.wikipedia.org/wiki/Jacques_Cohen

... staining techniques and co-culturing using enrichment techniques, will allow proper cultivation and sequencing of TM7x cells. ... Using the medium enrichment technique can reduce the TM7x containing cultures from a complex community to a dual species co culture ... A stable co-culture of the TM7x and XH001 can be obtained by using a medium, which has been developed to resemble the saliva, ... The co-culture can be passed through a 28-gauge needle, after which it can be filtered using a 0.22 µm filter. ...

https://en.wikipedia.org/wiki/TM7x

The technique will leverage computer assisted design models, co-culture media which support muscle and fat cells as well as ... Such a technique has already been perfected for industrial applications in manufacturing objects made out of plastic, nylon, ... Techniques of genetic engineering, such as insertion, deletion, silencing, activation, or mutation of a gene, are not required ... The major advantage is that this technique bypasses fetal bovine serum, meaning that no animal has to be killed in order to ...

https://en.wikipedia.org/wiki/Cultured_meat

Based on hypotheses about molecular regulators of valvular calcification and fibrosis, the lab uses in vitro co-culture systems ... It tries to identify though microgenomics techniques, the cellular and molecular differences in diseased vs. healthy heart ... Ongoing projects include utilization of novel biomaterials and measurement techniques, development of bioreactors, and ...

https://en.wikipedia.org/wiki/Craig_Alexander_Simmons

... cell culture techniques MeSH E05.200.249.374 - coculture techniques MeSH E05.200.249.437 - diffusion chambers, culture MeSH ... organ culture techniques MeSH E05.200.249.617 - tissue culture techniques MeSH E05.200.249.750 - tissue engineering MeSH ... fluorescent antibody technique MeSH E05.200.750.551.512.240.300 - fluorescent antibody technique, direct MeSH E05.200.750.551. ... patch-clamp techniques MeSH E05.200.750.132 - autoradiography MeSH E05.200.750.210 - bone demineralization technique MeSH ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(E05)

Immunological techniques are then commonly used to determine the species and/or serogroups of bacteria present in the sample. ... Although the plating method is quite specific for most species of Legionella, one study has shown that a coculture method that ... New techniques for the rapid detection of Legionella in water samples have been developed, including the use of polymerase ... "Isolation of Legionella anisa using an amoebic coculture procedure". Journal of Clinical Microbiology. 39 (1): 365-6. doi: ...

https://en.wikipedia.org/wiki/Legionella

Isolated cell culture, specifically co-culture of testis tissue, has been a useful technique for examining the influences of ... There are also techniques which are not practical or feasible in vivo which can now be explored. In vitro work is not without ... The development of this culture technique has taken place mainly with the use of animal models e.g. mice or rat testis tissue. ... Various categories of infertile men may benefit from advances in these techniques, especially those with a lack of viable ...

https://en.wikipedia.org/wiki/In_vitro_spermatogenesis

Bhatia also used co-cultures of more than one cell type to prevent dedifferentiation of the liver cells, building on the work ... She successfully applied techniques from photolithography to petri dishes, to create a substrate that would support growth of a ... She and her coworkers have also used techniques from 3D printing to create a lattice of sugar as a framework for a synthetic ...

https://en.wikipedia.org/wiki/Sangeeta_N._Bhatia

... is a technique of assisted reproductive technology. It involves placing a patient's fertilized ... Coculture can be an effective treatment for patients who have failed previous IVF cycles or who have poor embryo quality. A ... A typical Coculture cycle consists of the following steps: 1. Once a patient has been deemed an appropriate candidate for the ... The risks of Coculture are minimal. The procedure has been performed in over 1000 patients with no reported detrimental effects ...

https://en.wikipedia.org/wiki/Autologous_endometrial_coculture

The techniques are now used by many pregnant women and prospective parents, especially couples who have a history of genetic ... is co-cultured with a serum containing necessary signals, or is grafted in a three-dimensional scaffold to result. Embryonic ... It is a common technique to use mouse cells and other animal cells to maintain the pluripotency of actively dividing stem cells ... Weiss, Rick (2007-12-07). "Scientists Cure Mice Of Sickle Cell Using Stem Cell Technique: New Approach Is From Skin, Not ...

https://en.wikipedia.org/wiki/Embryonic_stem_cell

... scaffold techniques and scaffold-free techniques. Scaffold techniques include the use of solid scaffolds, hydrogels and other ... Spheroids co-cultured with tumor and healthy cells were used to simulate how cancerous cells interact with normal cells. ... Scaffold free techniques employ another approach independent from the use scaffold. Scaffold-free methods include e.g. the use ... These techniques have been applied to for in vitro disease models used to evaluate cellular responses to pharmaceutical ...

https://en.wikipedia.org/wiki/3D_cell_culture

A variety of techniques has been developed for the isolation and culturing of amniotic stem cells. One of the more common ... Co-culturing, i.e. mixing cells and plating them together, of human amniotic stem cells with neonatal rat ventricular myocytes ... Mesenchymal stem cells (MSCs) are highly abundant in the amniotic fluid and several techniques have been described for their ... In general, these types of techniques are considered to be potentially significant but further investigations are required. ...

https://en.wikipedia.org/wiki/Amniotic_stem_cells

Genome-based analytical techniques may prove especially useful for L. anisa, as a study of isolates from various locations in ... La Scola B, Mezi L, Weiller PJ, Raoult D (2001). "Isolation of Legionella anisa Using an Amoebic Coculture Procedure". J Clin ... In addition, such techniques greatly reduce the time required to obtain results. Infections may be asymptomatic, and are ... However, some research suggests L. anisa may require a co-culture method that accounts for the close relationship with amoebae ...

https://en.wikipedia.org/wiki/Legionella_anisa

He was a world authority on sex change operations, developing new techniques for transsexualism, embryo replacement, in vitro ... part of a team of clinicians and scientists which led to the world's first microinjection baby via human ampullary coculture[ ... and how he used cadavers to come up with his technique of sexual reassignment surgery. It also shows his assistant, Dr Lim, ...

https://en.wikipedia.org/wiki/Shan_Ratnam

Recently, exometabolomics has been used to design co-culture systems. Because the analysis of extracellular metabolites allows ... Footprinting, in combination with other techniques, for early recognition of outbreak and strain characterization. Studying ... Exometabolomic techniques have been used in the following fields: Metabolite utilization to annotate function of unknown genes ... "Liquid chromatography-mass spectrometry for metabolic footprinting of co-cultures of lactic and propionic acid bacteria". ...

https://en.wikipedia.org/wiki/Exometabolomics

Other microscopy techniques are commonly used in tandem with NanoSIMS that allow for multiple types of information to be ... NanoSIMS can be used for pure cultures, co-cultures, and mixed community samples. The first use of NanoSIMS in biology was by ... This technique can be used to study nutrient exchange. The mouse gut microbiome was investigated to determine which microbes ... Unlike other imaging techniques, where 13C14N and 12C15N cannot be independently measured due to nearly identical masses,, ...

https://en.wikipedia.org/wiki/Nanoscale_secondary_ion_mass_spectrometry

Pre-implantation techniques include PGD, but also sperm sorting. Other assisted reproduction techniques include: Mitochondrial ... Autologous endometrial coculture is a possible treatment for patients who have failed previous IVF attempts or who have poor ... The latter technique has proved to be less deleterious for the embryo, therefore it is advisable to perform the biopsy around ... This technique is used in cases when mothers carry genes for mitochondrial diseases. The therapy is approved for use in the ...

https://en.wikipedia.org/wiki/Assisted_reproductive_technology

This technique is used in cases when mothers carry genes for mitochondrial diseases. The therapy is approved for use in the ... Autologous endometrial coculture is a possible treatment for patients who have failed previous IVF attempts or who have poor ... Techniques usually used in in vitro fertilization include: *Transvaginal ovum retrieval (OVR) is the process whereby a small ... Less commonly used techniques in in vitro fertilization are: *Assisted zona hatching (AZH) is performed shortly before the ...

https://en.m.wikipedia.org/wiki/Assisted_reproductive_technology

The technique can be applied to a large number of cells to produce stem cells for medical purposes on an industrial scale. ... While initial studies revealed that co-culturing epithelial cells with Swiss 3T3 cells J2 was essential for CRC induction, with ... The technique enables researchers to search large libraries of antibodies and quickly select the ones with a desired biological ... Three techniques are widely recognized: Transplantation of nuclei taken from somatic cells into an oocyte (egg cell) lacking ...

https://en.wikipedia.org/wiki/Induced_stem_cells

Clones of the S and L types could co-exist stably in co-culture with each other, indicating they occupied distinct niches in ... A later study by Leiby and Marx that used more advanced techniques showed that much of the decay Cooper and Lenski had ... and as technology and methodological techniques have advanced. The use of E. coli as the experimental organism has allowed many ... by Eric Quandt in the lab of Jeffrey Barrick at the University of Texas at Austin described the application of a new technique ...

https://en.wikipedia.org/wiki/E._coli_long-term_evolution_experiment

In 2016, researchers developed a technique that used cancer cells' RNA to produce T cells and an immune response. They encased ... They then produce pure cultures of lymphocytes that can be tested for reactivity against other tumors, in coculture assays. ... As of 2015 the technique had expanded to treat cervical cancer, lymphoma, leukemia, bile duct cancer and neuroblastoma and in ...

https://en.wikipedia.org/wiki/Adoptive_cell_transfer

This technique is also used in sheep, pigs and other animals. See In animals. Oocytes are classified depending on their ... Kannan S, Mehta A, Simha V, Reddy OK, Kaur BP, Onteru SK, Singh D (2014). "Photoinduction of granulosa cell and oocyte co-culture ... The best oocytes are chosen to be matured in the hope of then being implanted using in vitro fertilisation techniques. The ... IVM is not an entirely perfected technique. Pregnancy rates are lower in IVM than in standard IVF. There is also research ...

https://en.wikipedia.org/wiki/In_vitro_maturation

Optical detection includes fluorescence-based techniques, chemiluminescence-based techniques, and surface plasmon resonance ( ... Micropatterned co-cultures have also contributed to bio-MEMS for tissue engineering to recapitulate in vivo conditions and 3D ... Conventional micromachining techniques such as wet etching, dry etching, deep reactive ion etching, sputtering, anodic bonding ... Techniques for micropatterning paper include photolithography, laser cutting, ink jet printing, plasma treatment, and wax ...

https://en.wikipedia.org/wiki/Bio-MEMS

Mixed co-culture is the simplest co-culture method, where two types of cells are in direct contact within a single culture ... This technique has demonstrated a stark difference in the sensitivity of the peripheral terminals compared to the neuronal cell ... After gelation, the agarose microgels will serve as a 3D microenvironment for cell co-culture. Segregated co-culture is also ... co-culture, and segregated (or indirect) co-culture. The use of conditioned media, where the cultured medium of one cell type ( ...

https://en.wikipedia.org/wiki/Microfluidic_cell_culture

Two major alternatives to in vivo animal testing are in vitro cell culture techniques and in silico computer simulation. ... circulatory flowrate and zonation and multi cellular co-cultures," the biochips have set themselves apart from basic cell ... One model is the "lung-on-a-chip". This combines microfabrication techniques with modern tissue engineering and mimics the ... 33 (439). Russell, W.M.S. and Burch, R.L., (1959). The Principles of Humane Experimental Technique, Methuen, London. ISBN 0- ...

https://en.wikipedia.org/wiki/Alternatives_to_animal_testing

Most significantly, the monolayers can be characterized with MALDI mass spectrometry in a technique known as SAMDI-MS, which ... Several strategies using this approach were studied in the context of cell signaling, migration, and co-culture. Subsequent ... Most recently, Mrksich's group has focused on developing a technique for assembling large molecular structures with perfectly ...

https://en.wikipedia.org/wiki/Milan_Mrksich

Prichard, JF; Thibodeaux, JK; Pool, SH; Blakewood, EG; Menezo, Y; Godke, RA (April 1992). "In-vitro co-culture of early stage ... and granulosa lutein cells to avoid microbial contamination during human oocyte retrieval for assisted reproductive techniques ...

https://en.wikipedia.org/wiki/Antibiotic-Antimycotic

Techniques for cell isolation depend on the cell source. Centrifugation and apheresis are techniques used for extracting cells ... Collagen/hyaluronic acid scaffolds have been used for modeling the mammary gland In Vitro while co-coculturing epithelial and ... This technique does not require the use of a solid porogen like SCPL. First, a synthetic polymer is dissolved into a suitable ... Each of these techniques presents its own advantages, but none are free of drawbacks. Molecular self-assembly is one of the few ...

https://en.wikipedia.org/wiki/Tissue_engineering

Thus, a combination of the flap and AVB techniques can maximize the growth rate and vascular contact of the bioreactor as ... These cells secrete growth factors that promote repair and can be co-cultured with stem cells to improve tissue formation. ... Service, Robert F. (29 July 2005). "Technique Uses Body as 'Bioreactor' to Grow New Bone". Science. 309 (5735): 683. doi: ... A Novel Technique for Periosteal Harvest". Journal of Investigative Surgery. 17 (4): 229-233. doi:10.1080/08941930490472073. ...

https://en.wikipedia.org/wiki/In_vivo_bioreactor

This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal ... 7] Based on the evaluation of the babies born, no abnormalities attributable to the ROSI technique have been identified. [8][9] ... This technique can be used to enable genetic fatherhood to some men who have no spermatozoa in the ejaculate (azoospermia) and ... The technique was developed by Gianpiero Palermo at the Vrije Universiteit Brussel, in the Center for Reproductive Medicine ...

https://en.wikipedia.org/wiki/Intracytoplasmic_sperm_injection?wprov=sfla1

have co-cultured aquatic species for centuries in marine, brackish and fresh water environments.[1][3] Fish, shellfish and ... Ideally, the co-cultured species each yield valuable commercial "crops".[2] IMTA can synergistically increase total output, ... IMTA is a specialized form of the age-old practice of aquatic polyculture, which was the co-culture of various species, often ... Pacific SEA-lab is researching and is licensed for the co-culture of sablefish, scallops, oysters, blue mussels, urchins and ...

https://en.wikipedia.org/wiki/Integrated_Multi-trophic_Aquaculture

Controlled-rate and slow freezing, also known as slow programmable freezing (SPF),[14] is a set of well established techniques ... The main techniques to prevent cryopreservation damages are a well-established combination of controlled rate and slow freezing ... Several independent studies have provided evidence that frozen embryos stored using slow-freezing techniques may in some ways ... stepwise equilibration and slow cooling as an efficient technique for large-scale cryopreservation of human embryonic stem ...

https://en.wikipedia.org/wiki/Cryopreservation

"Cost-effectiveness modelling of recombinant FSH versus urinary FSH in assisted reproduction techniques in the UK". Human ... Autologous endometrial coculture. *Cytoplasmic transfer. *Embryo transfer. *Gestational carrier. *In vitro maturation ...

https://fa.wikipedia.org/wiki/منوتروپین?match=en

The technique is recommended for couples who will not accept a child of the undesired gender.[27][28] ... The IVF/PGD technique is favored over the Ericsson method because of the stricter control of the offspring gender in the ... Sperm sorting is an advanced technique that sorts sperm "in vitro" by flow cytometry. This shines a laser at the sperm to ... Sperm sorting utilizes the technique of flow cytometry to analyze and 'sort' spermatozoa. During the early to mid-1980s, Dr. ...

https://en.wikipedia.org/wiki/Sex_selection

Algae can be converted into various types of fuels, depending on the technique and the part of the cells used. The lipid, or ... Mackay, S.; Gomes, E.; Holliger, C.; Bauer, R.; Schwitzguébel, J.-P. (2015). "Harvesting of Chlorella sorokiniana by co-culture ... Using these techniques, the company states that lipid production could be increased several-fold, potentially making algal ... Algenol's technology produces high yields and relies on patented photobioreactors and proprietary downstream techniques for low ...

https://en.wikipedia.org/wiki/Algal_fuel

This technique is used in order to prepare the gametes for the obtention of embryos that may be transferred to a maternal ... The technique was developed by Gianpiero Palermo at the Vrije Universiteit Brussel, in the Center for Reproductive Medicine ... A systematic meta-analysis of 24 estimates of DNA damage based on a variety of techniques concluded that sperm DNA damage ... The first child born from a gamete micromanipulation (technique in which special tools and inverted microscopes are used that ...

https://en.wikipedia.org/wiki/Intracytoplasmic_sperm_injection

Pregnancy urine dipstick tests are based on the lateral flow technique. *The urine test may be a chromatographic immunoassay or ... Autologous endometrial coculture. *Cytoplasmic transfer. *Embryo transfer. *Gestational carrier. *In vitro maturation ...

https://en.wikipedia.org/wiki/Beta-HCG

Ten Broek, R. P. G.; Kok- Krant, N.; Bakkum, E. A.; Bleichrodt, R. P.; Van Goor, H. Different surgical techniques to reduce ... 自体子宫内膜共同培养（英语：Autologous endometrial coculture） ... Gurgan T, Salman C, Demirol A. Pregnancy and assisted reproduction techniques in men and women after cancer treatment. Placenta ... a successful technique but with an accompanying high risk of hypertensive disorders during pregnancy. Hum. Reprod. March 2006, ...

https://zh.wikipedia.org/wiki/女性不孕症

IVF and related techniques (ICSI, ZIFT, GIFT) are called assisted reproductive technology (ART) techniques. ... ICSI technique is used in case of poor semen quality, low sperm count or failed fertilization attempts during prior IVF cycles ... This technique involves an injection of a single healthy sperm directly injected into mature egg. The fertilized embryo is then ... ART techniques generally start with stimulating the ovaries to increase egg production. After stimulation, the physician ...

https://en.wikipedia.org/wiki/Infertility

... the findings showed reduced levels of tumor necrosis factor-alpha and interleukin-10 in T cell-microglia co-culture. The ... Peripherally inserted central catheter (Seldinger technique). *Central venous catheter. Other equipment. *Infusion pump ...

https://en.wikipedia.org/wiki/Intravenous_immunoglobulin

The co-cultured species should not just be biofilters, but harvestable crops of commercial value. A working IMTA system should ... including the development of modern broiler rearing techniques, the adoption of the Cornish Cross broiler, the use of laying ... result in greater production for the overall system, based on mutual benefits to the co-cultured species and improved ecosystem ...

https://en.wikipedia.org/wiki/Intensive_animal_farming

However, they hardly form blooms with sufficiently high concentration to be visible without enrichment techniques. Purple ... "Photoproduction of H2 from acetate by syntrophic cocultures of green sulfur bacteria and sulfur-reducing bacteria". Archives of ...

https://en.wikipedia.org/wiki/Purple_bacteria

Co-cultures consisting of pericytes and endothelial cells induce the expression of TIMP-3 by pericytes, while endothelial cells ... Examination of cathepsin activity by using chemical probes and in vivo imaging techniques demonstrated an increase in cathepsin ...

https://en.wikipedia.org/wiki/Proteases_in_angiogenesis

These are generally performed using tissue culture methods that rely on aseptic technique. Aseptic technique aims to avoid ... Duell BL, Cripps AW, Schembri MA, Ulett GC (2011). "Epithelial cell coculture models for studying infectious diseases: benefits ... This technique is known as two-dimensional (2D) cell culture, and was first developed by Wilhelm Roux who, in 1885, removed a ... The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the ...

https://en.wikipedia.org/wiki/Cell_culture

This is one potential distinction from the age-old practice of aquatic polyculture, which could simply be the co-culture of ... "Aquaculture's growth continuing: improved management techniques can reduce environmental effects of the practice. (UPDATE)." ... Onshore recirculating aquaculture systems, facilities using polyculture techniques, and properly sited facilities (for example ... A working IMTA system can result in greater total production based on mutual benefits to the co-cultured species and improved ...

https://en.wikipedia.org/wiki/Aquaculture

Therefore, a number of techniques are employed by microbiologists when culturing anaerobic organisms, for example, handling the ... "Isolation and culture of Methanobrevibacter smithii by co-culture with hydrogen-producing bacteria on agar plates". Clinical ... bacteria in a glovebox filled with nitrogen or the use of other specially sealed containers, or techniques such as injection of ...

https://en.wikipedia.org/wiki/Anaerobic_organism

More recently, however, studies in 3D human coculture are proving to be an effective method with which to study cancer risks in ... Novel multicolor fluorescence painting techniques of human chromosomes have clearly demonstrated that high-LET α-particles and ...

https://en.wikipedia.org/wiki/Radiobiology_evidence_for_protons_and_HZE_nuclei

In order to properly seed the 2D cell culture, a microcontact printing technique was used to lay out a fibronectin "brick wall ... Hermanns MI, Fuchs S, Bock M, Wenzel K, Mayer E, Kehe K, Bittinger F, Kirkpatrick CJ (April 2009). "Primary human coculture ... For example, animal models are often subjected to mechanical or chemical techniques that simulate human injuries. There are ... conventional laboratory techniques usually focus on 2D structures, such as petri-dish that lacks capability to recapitulate ...

https://en.wikipedia.org/wiki/Organ-on-a-chip

Emerging optic-based techniques, for example, the pH optrode (or optode), which can be integrated into a self-referencing ... "Membrane potential depolarization causes alterations in neuron arrangement and connectivity in cocultures". Brain and Behavior ... These techniques allow further characterization of the wound electric fields/currents at cornea and skin wounds, which show ... The gold standard techniques to quantitatively extract electric dimensions from living specimens, ranging from cell to organism ...

https://en.wikipedia.org/wiki/Bioelectricity

This study demonstrated that both the monoculture extracts of the native strain T. maxima and its co-culture with P. carneus ... recovery of gold from electronic waste using mycofiltration techniques. Fungi are amongst the primary saprotrophic organisms in ... Chan-Cupul W, Heredia-Abarca G, Rodríguez-Vázquez R (2016). "Atrazine degradation by fungal co-culture enzyme extracts under ... a prospective environmental friendly technique of bioseparation and dewatering of domestic wastewater sludge". Environmental ...

https://en.wikipedia.org/wiki/Mycoremediation

Psychiatry related information on Coculture Techniques. *The von Ebners-like protein mRNA was induced after 4 h of co-culture ... Chemical compound and disease context of Coculture Techniques. *The coculture of cells expressing the HIV-1 envelope ... Gene context of Coculture Techniques. *Additionally, in cocultures in which spleen cells were derived from receptor-deficient ... Disease relevance of Coculture Techniques. *Antiestrogen-induced TGF-beta from MCF-7 cells inhibits the growth of an estrogen ...

http://www.wikigenes.org/e/mesh/e/23239.html

The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and ... The liquid overlay technique is the key to formation of co-culture spheroids consisting of primary osteoblasts, fibroblasts and ...

https://www.promocell.com/resources/the-liquid-overlay-technique-is-the-key-to-formation-of-co-culture-spheroids-consisting-of-primary-osteoblasts-fibroblasts-and-endothelial-cells/

Results showed that cocultured cells expressed much higher N-cadherin than monocultured cells after 24 and 48 h of culture. We ... Coculture Techniques * Endothelium, Vascular / cytology * Endothelium, Vascular / physiology* * Humans * Middle Aged * ... In addition, more beta-catenin was found to translocate into the cocultured cells nuclei and more T cell factor-1 (TCF-1) were ... Taking our findings together it can be concluded that cocultures of HBMSCs with HUVECs increased N-cadherin expression and ...

https://pubmed.ncbi.nlm.nih.gov/20664068/?dopt=Abstract

... J Neurochem. 2011 Nov;119(3 ... To this end, differentiated rat NPCs were co-cultured with rat BMECs and shown to induce BBB properties such as elevated trans- ... Coculture Techniques * Endothelium, Vascular / cytology* * Endothelium, Vascular / physiology* * Humans * Male * ... Taken together, these results demonstrate that NPCs are an attractive alternative to primary neural cells for use in BBB co-culture ...

https://pubmed.ncbi.nlm.nih.gov/21854389/?dopt=Abstract

Coculture Techniques. Ganglia, Sensory / cytology*. Intracellular Space / drug effects, metabolism. Neurons / cytology, drug ... Calcium sensitivity of petrosal ganglion neurons (PGNs) to chemical stimuli with and without PC-12 cells in co-culture instead ...

http://www.biomedsearch.com/nih/Calcium-Sensitivity-Hypoxia-in-PGNs/19536473.html

Conventional G-banding technique was used to analyze parental MG63, MGRWV, MGLN, and MGC4-2 cells. The cytogenetics of MG63 ... Three-dimensional coculture of PCa and bone stromal cells in RWV. LN, C4-2, MG-63, HFOB, and HS27A cells were seeded in T- ... Cocultured organoids were harvested after 2 or 3 wk with dispase (5 mg/mL; Roche) or trypsin (0.25% v/v; Invitrogen). Cells ... We cocultured MG63, a human osteosarcoma cell line, or HS27A, a normal immortalized human osteoblastic cell line, with LN and ...

http://cancerres.aacrjournals.org/content/68/23/9996

Molecular techniques.DNA and RNA extractions from cheese samples (0.5 g each), PCR and reverse transcription (RT)-PCR ... Production of freeze-dried kefir coculture.Kefir coculture isolated from a commercial kefir drink was used in the present study ... Addition of a freeze-dried kefir starter coculture, the initial concentration of the freeze-dried kefir coculture, the salting ... Evaluation of Freeze-Dried Kefir Coculture as Starter in Feta-Type Cheese Production. Y. Kourkoutas, P. Kandylis, P. Panas, J. ...

https://aem.asm.org/content/72/9/6124

Autologous Endometrial Coculture is a technique of assisted reproductive technology. It involves placing a patients fertilized ... Coculture can be an effective treatment for patients who have failed previous IVF cycles or who have poor embryo quality. A ... A typical Coculture cycle consists of the following steps: 1. Once a patient has been deemed an appropriate candidate for the ... The risks of Coculture are minimal. The procedure has been performed in over 1000 patients with no reported detrimental effects ...

https://en.wikipedia.org/wiki/Autologous_endometrial_coculture

A Novel Technique for Creating Multi-compartment, Customizable Scaffolds for Tissue Engineering, Isolation and Culture of ... culture techniques include Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow, ... Cell Co-culture Patterning Using Aqueous Two-phase Systems. John P. Frampton1, Joshua B. White1, Abin T. Abraham1, Shuichi ... Mesenchymal Stem Cell Isolation from Pulp Tissue and Co-Culture with Cancer Cells to Study Their Interactions. Ayşegül Doğan1, ...

https://www.jove.com/keyword/culture+techniques

We cocultured SVCs that include macrophages with adipose tissue from lean C57BL/6J mice in the presence of an adrenergic ... Tissues were handled using sterile techniques and minced into fine (,10 mg) pieces. For SVC isolation, minced samples were ... Coculture experiments. To isolate SVCs, perigonadal adipose tissue was isolated from high-fat diet-induced obese C57BL/6J male ... SVCs were cocultured with adipose tissue pieces for 24 hours in DMEM containing 10% FBS, 1% penicillin, and 2% fatty acid-free ...

https://www.jci.org/articles/view/42845

A) 3-wk-old myelinating OL-DRG cocultures stained for MBP, Olig2, and neurofilament. Coc ... Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. ( ... Coculture Techniques. *Cytoskeleton/metabolism. *Ganglia, Spinal/cytology. *Heterocyclic Compounds with 4 or More Rings/ ... fig5: Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. (A) 3-wk-old myelinating OL-DRG cocultures stained ...

https://openi.nlm.nih.gov/detailedresult.php?img=PMC2542477_jcb1821171f05&req=4

Adherent MN were cocultured in the presence of varying concentrations of 1α,25(OH)2D3 in 0.1% ethanol for 72 h. Staining for ... Infection and subsequent handling of cultures was according to techniques described previously (31). MN preparations were ... 1⇑B). To investigate the relationship between RLU and CFU, we cocultured the PBMC of six donors with MTB-lux at a MOI of 0.1:1 ... The coculture of BCG-lux with PBMC is a convenient assay system, but we wished to ensure our observations were generalizable to ...

https://www.jimmunol.org/content/178/11/7190?ijkey=e83330a6f0faf65032c68d61cfd8f364ed46450d&keytype2=tf_ipsecsha

Co-culture of MEFs and HUVECs by encapsulating them together in 0.07% RAD16-I. Molecular Biology Techniques. RT-PCR. Western ... Co-culture of MEFs and HUVECs. Co-culture of MEFs and HUVECs by loading a HUVECs suspension over a MEFs culture in 0.07% RAD16- ... Co-culture MEFs + HUVECs. HUVECs loaded over MEFs. Co-culture MEFs + HUVECs. HUVECs loaded over MEFs. Collagen X Expression. WB ... Analysis of the Gene Expression of Co-Cultures of MEFs and HUVECs in RAD16-I Ortega et al. 2004. Coll I. Coll I. Coll II. Coll ...

https://prezi.com/t3gho1nwcspv/effect-of-the-3d-microenvironment-on-the-gene-expression-of/

1.Laboratoire Mer Molécules Santé (MMS), FR CNRS 3473 IUML, Faculté des Sciences et TechniquesUniversité du MaineLe MansFrance ... Allelopathic tests were realized by co-culturing these microalgae with H. ostrearia in batch and in semi-continuous mode, based ... 3.Laboratoire Mer Molécules Santé, FR CNRS 3473 IUML, Faculté des Sciences et TechniquesUniversité de NantesNantesFrance ... Does allelopathy affect co-culturing Haslea ostrearia with other microalgae relevant to aquaculture?. ...

https://link.springer.com/article/10.1007%2Fs10811-015-0779-y

Coculture Techniques. Female. Male. Nerve Regeneration. Neurites / physiology. Olfactory Nerve / cytology*, immunology, ... Rolf B1.T cells support the regrowth of neurites from adult retinal ganglion cells in vitro in a heterologous co-culture system ...

http://www.biomedsearch.com/nih/Spontaneous-immortalisation-ensheathing-cells-from/8833195.html

Co-culture experiments that exposed healthy donor PBMCs to lung cancer cells in vitro were performed to evaluate the effect on ... Further mechanism dissection showed that co-culturing T cells with RCC cells could produce more IGF-1 and FGF-7, which may ... Mechanism dissection suggested that co-culturing T cells with RCC cells released more T cell attraction factors, including IFN- ... induced similar changes in cytokine gene and protein expression by healthy donor PBMCs in an in vitro co-culture system. We ...

http://www.cancerindex.org/geneweb/CCL3.htm

... but did not protect co-cultures, when used alone or in combination. Application of GSSG prevented degeneration in co-cultures, ... Cell viability was measured biochemically and by quantitative neurite imaging, siRNA techniques were also used. We initially ... In these co-cultures, MPTP was metabolized to 1-methyl-4-phenyl-pyridinium (MPP(+) ) by the glial cells, and the toxic ... Moreover, available test systems rarely reflect functional roles of co-cultured glial cells. There is no human in vitro ...

https://www.sigmaaldrich.com/catalog/papers/25989025

Pre-degenerated peripheral nerves co-cultured with bone marrow-derived cells: a new technique for harvesting high-purity ...

https://www.nature.com/articles/nn0909-1085?error=cookies_not_supported&code=a7dbe429-3577-4030-ba6c-1e628a9ffe9f

... using that technique, Rowbotham was able to grow L. pneumophila from human feces (43). Moreover, the amoebal coculture is a ... Amoebal Coculture. By amoebal coculture, we recovered seven (1.6%) strains of ARB from nasal swabs of 4 of 444 participants. ... Amoebal Coculture. The microplates were centrifuged at 2,879 X g (4,000 rpm) for 30 minutes and incubated at 32°C in a ... The amoebal cocultures were subcultured on fresh amoebae on days 4 and 7 (samples from controls and patients) or on day 6 ( ...

https://wwwnc.cdc.gov/eid/article/10/3/02-0792

Thus, amoebal coculture allows the recovery of new bacterial species from heavily contaminated samples and might be a valuable ... Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human ... To isolate new ARB, we performed amoebal coculture from 444 nasal samples. We recovered 7 (1.6%) ARB from 444 nasal swabs, ... using that technique, Rowbotham was able to grow L. pneumophila from human feces (43). Moreover, the amoebal coculture is a ...

https://wwwnc.cdc.gov/eid/article/10/3/02-0792_article

An internal reference technique for accurately quantifying specific mRNAs by real-time PCR with application to the tceA ... S1 in the supplemental material); therefore, cocultures were maintained with 0.6 mM TCE and 4 mM butyrate. After this coculture ... mccartyi-containing cocultures (4.2 to 6.8 μmol day−1 [5, 7, 14-16]). The cell yield of strain 195 in this coculture [(1.1 ± ... in the cocultures growing on butyrate. In contrast, when the coculture was maintained on crotonate, the cell number ratio was ...

https://aem.asm.org/content/81/6/2015

Retinal Coculture Techniques. A new review discusses retinal coculture models of the neural retina, retinal pigment epithelium ...

https://stemcellsportal.com/taxonomy/term/324

Spinal cord - motor cortex co-culture model - a new technique to study neuronal regeneration in vitro Meeting Abstract ... Conclusions: This new slice co-culture model provides an important in vitro tool for a variety of questions in the field of ... We report here about a new slice co-culture model for the study of neuronal regeneration. ...

http://www.egms.de/static/en/meetings/dgnc2010/10dgnc332.shtml

Coculture Techniques. 2. 2014. 1346. 0.090. Why? Gene Knockdown Techniques. 1. 2012. 1689. 0.090. Why? ...

https://connects.catalyst.harvard.edu/Profiles/display/person/104274/network/researchareas/details

24.2.4 Patterned Co-cultures Using LbL Technique 534. 24.3 Controlled Drug Delivery for Nerve Regeneration 536 ... In the last 15 years the LbL technique has seen considerable developments and has now reached a point where it is beginning to ... The book gives a thorough overview of applications of the LbL technique in the context of bioengineering and biomedical ... 14.2 Fabrication of Polysaccharide Capsules by the LbL Technique 296. 14.2.1 Natural Charged Polysaccharides Used in LbL ...

https://www.wiley.com/en-us/Layer+by+Layer+Films+for+Biomedical+Applications-p-9783527675869

Advanced training in cell culture and co-culturing techniques. *Immunohistochemical and immunofluorescence staining techniques ... Opportunities to learn RNA, DNA and protein assessment techniques. * ... as well as endocrine and neurotoxicology techniques. ...

https://www.niehs.nih.gov/careers/research/postdoc-training/index.cfm

Cell-culture-techniques; Cell-cultures; Lung-cells; Animals; Humans; Laboratory-animals; Author Keywords: Coculture; Gene ... As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer ... expressed in both cell types in coculture than in monoculture. When reduced to only those genes involved in inflammation and ... induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene ...

http://www2a.cdc.gov/nioshtic-2/BuildQyr.asp?s1=+%27ultrafine%2A%27+OR+%28%27nano%2A%27+AND+%27particle%2A%27%29+OR+%27nanotech%2A%27+OR+%27nanomaterial%2A%27+OR+%27nanoparticle%2A%27+OR+%27nanotube%2A%27+&f1=%2A&Startyear=&terms=3&Adv=1&ct=&B1=Search&Limit=25000&Sort=DP+DESC&whichdate=DP&D1=10&EndYear=&PageNo=72&RecNo=717&View=f&

Two different cell populations can be labeled with different ViaFluor® SE dyes, mixed together, and tracked in co-culture over ... For two-color co-culture, stain each population separately with a different ViaFluor® dye color. ... Luminescence: Mechanisms and Applications for 4 Prominent Techniques Subscribe & Follow. Sign up for news & promotions:. ...

https://biotium.com/tech-tips/tech-tip-using-viafluor-se-stains-for-cell-tracing-and-co-culture/

21st National level training programme on In-Vitro Fertilization and Embryo Co-Culture: TANUVAS, Chennai, India. Feb 24 to Mar ... Department lab rotation to understand advanced reproductive techniques in the filed of Reproductive Biology. (CASA, Hamster ... Preparation and maintenance of oviduct epithelial cells, cumulus cells etc., for embryo co-culture. ... "In-Vitro fertilization and Embryo Co-Culture" for 21 days starts from Feb 24 to Mar 15, 2016. ...

https://ivf.net/ivf/21st-national-level-training-programme-on-in-vitro-fertilization-and-embryo-co-culture-tanuvas-chenn-o8648.html

18th National level training programme on In-Vitro Fertilization and Embryo Co-Culture: TANUVAS, Chennai, India. Aug 01, 2014 ... Department lab rotation to understand advanced reproductive techniques in the filed of Reproductive Biology. (CASA, Hamster ... Preparation and maintenance of oviduct epithelial cells, cumulus cells etc., for embryo co-culture. ... "In-Vitro fertilization and Embryo Co-Culture" for 21 days starts from Aug 01,2014 to Aug 23, 2014. ...

https://ivf.net/ivf/18th-national-level-training-programme-on-in-vitro-fertilization-and-embryo-co-culture-tanuvas-chenn-o8143.html

This study compared global multi-walled carbon nanotube (MWCNT)-induced gene expression from human lung epithelial and microvascular endothelial cells in monoculture and coculture with gene expression from mouse lungs exposed to MWCNT. (cdc.gov)
This study is based on an in vitro coculture model consisting of outgrowth endothelial cells and primary osteoblasts and is currently being used in different studies of bone repair processes with special regard to angiogenesis and osteogenesis. (hindawi.com)
Coculture systems of OECs and pOBs positively influence the angiogenic potential of endothelial cells by inducing the formation of angiogenic structures in long-term cultures. (hindawi.com)
In the present study a coculture system of hepatocytes and endothelial cells was established to investigate the communication between parenchymal and nonparenchymal cells. (elsevier.com)
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure. (cdc.gov)
This study identified differential gene regulation in human small airway epithelial cells (SAECs) grown either in monoculture or in coculture with human microvascular endothelial cells following exposure of the SAECs to multiwalled carbon nanotubes (MWCNTs). (cdc.gov)

The transcriptome of strain 195 during exponential growth in the coculture indicated increased ATP-binding cassette transporter activities compared to the pure culture, while the membrane-bound energy metabolism related genes were expressed at stable levels. (asm.org)

In vitro fertilization with coculture has been utilized in animal in vitro embryo culture systems for over 30 years and more recently in some clinical human in vitro fertilization laboratories as well. (advancedfertility.com)
Some studies have demonstrated improved pregnancy rates and delivery rates with utilization of coculture for human in vitro fertilization. (advancedfertility.com)
There is not universal agreement that coculture is necessary to provide optimal pregnancy rates from human in vitro fertilization. (advancedfertility.com)
One school says that coculture can be of a benefit for some patients undergoing in vitro fertilization. (advancedfertility.com)
Clinical in vitro fertilization programs that are utilizing coculture for their human IVF generally use one of three cell types. (advancedfertility.com)
citation needed] In 1982, Cohen joined Bourn Hall Clinic as an Embryologist, working with Patrick Steptoe and Robert G. Edwards on techniques geared towards improving human conception through in vitro fertilization (IVF). (wikipedia.org)

These results, combined with our previous observations, support the concept of permanent genetic and behavioral changes of PCa epithelial cells after being either cocultured with prostate or bone stromal cells as three-dimensional prostate organoids or grown as tumor xenografts in mice. (aacrjournals.org)

The present study focused on the investigation of the roles of neural (N)-cadherin in early differentiation of HBMSCs in direct-contact cocultures with HUVECs for 24 and 48 h. (nih.gov)
A neutralizing monoclonal antibody against epimorphin (MC-1) efficiently blocked the development of hollow sphere structures from A818-6 cells.Coculture of A818-6 cells with fibroblasts stimulated the development of hollow sphere structures in general and increased differentiation in 5-6-d-old hollow spheres.A818-6 hollow sphere development in the presence of fibroblasts was also blocked by MC-1. (nih.gov)
Coculture of A818-6 cells with fibroblasts stimulated the development of hollow sphere structures in general and increased differentiation in 5-6-d-old hollow spheres. (nih.gov)
To characterize some of the cell-cell connections that may be important for MO differentiation we cocultured human MO with lymphocytes and/or with platelets. (uni-regensburg.de)
Previous evidence supportsthe potential for direct chondrogenic differentiation of stem cells by coculturing with a terminally differentiated chondrocyte cell population ( 11 , 12 ). (pnas.org)
However, these protocols all require culture at very low densities and cell-cell/matrix interactions for neuronal differentiation, and the mechanisms underlying neuronal lineage commitment induced by these techniques remain unclear. (biologists.org)

Confocal laser scanning analysis of coculture experiments of A818-6 cells with Kif-5 fibroblasts transduced with the gene coding for the EGFP. (nih.gov)
The student will learn several state-of-the art techniques, including droplet microfluidics, enzymatic hydrogel crosslinking, (3D) stem cell culture, immunohistochemistry, and confocal fluorescence imaging and apply this skill set in a top-level institute to perform pioneering work on artificial cells. (utwente.nl)
GE Healthcare is sponsoring a new, free educational webinar , "Magnetic 3D Bioprinting of Cells: Overcoming Imaging Obstacles in Spheroids," which will discuss magnetic 3D bioprinting of spheroids and rings, offer tips for high-throughput confocal imaging of 3D cells within Greiner Bio-One 384 well black µClear® flat bottom cell-repellent surface microplates, techniques for bioprinting cell co-cultures, and rapid 3D model formation with easy post-culture analysis. (prweb.com)
One brain cell line was partially characterized by DNA sequencing, morphology, antigen expression using flow cytometry, confocal microscopy, and electrophysiology using the patch clamp technique. (mdc-berlin.de)

Primary CLL cells were cocultured in vitro with CD40L-expressing stroma to mimic the prosurvival conditions present in lymphoid tissue. (aacrjournals.org)

11 However, in contrast to the mechanical injury of the endothelium applied in the transfilter coculture technique, 11 only an indirect injury is performed by leukocyte attack (LA) with human monocytes or human CD4 + lymphocytes in the 3DLA model. (ahajournals.org)

Using a cutoff of 10% false discovery rate and 1.5 fold change, we determined that there were more concordant genes (gene expression both up- or downregulated in vivo and in vitro) expressed in both cell types in coculture than in monoculture. (cdc.gov)
When reduced to only those genes involved in inflammation and fibrosis, known outcomes of in vivo MWCNT exposure, there were more disease-related concordant genes expressed in coculture than monoculture. (cdc.gov)
SAEC genes that either changed their regulation direction from upregulated in monoculture to downregulated in coculture (or vice versa) or had a more than a two-fold changed in the same regulation direction were identified. (cdc.gov)
The direction and fold-change of this differential gene regulation suggests that toxicity testing in monoculture may exaggerate cellular responses to MWCNTs, and coculture of cells may provide a more in-depth assessment of toxicological responses. (cdc.gov)
These results demonstrate that the osteoblast/LNCaP coculture system is a better in vitro screen than monoculture to identify proliferation-independent agents for the treatment of prostate cancer bone metastases, and TG is such an agent. (elsevier.com)

Lentiviral infection of purified OPC cultures resulted in significant cell death (unpublished data), whereas infection of OPC in cocultures with DRG neurons was well tolerated. (nih.gov)
Incubation of cocultures with lentivirus resulted in the preferential infection of OPC rather than neurons, as indicated by GFP expression (Fig. S3, available at http://www.jcb.org/cgi/content/full/jcb.200802091/DC1). (nih.gov)
In contrast, the presence of L1-Fc, the extracellular portion of a contactin ligand expressed on axons, enhanced survival and additionally promoted myelination in cocultures of neurons and oligodendrocytes. (jneurosci.org)

Another issue is that depending on the source of cells used for the coculture there are concerns about transmission of infectious diseases such as viruses from the cell line to the developing embryos. (advancedfertility.com)
Cohen is known for the application of micromanipulation techniques to operate on eggs, sperm and embryos. (wikipedia.org)

Human bone stromal cells, after three-dimensional coculture with human prostate cancer (PCa) cells in vitro , underwent permanent cytogenetic and gene expression changes with reactive oxygen species serving as mediators. (aacrjournals.org)
In this communication, we showed marked cytogenetic, gene expression and behavioral changes in bone cells cocultured under three-dimensional conditions with PCa cells, with ROS serving as the critical mediators. (aacrjournals.org)
As coculture gene expression better correlated with in vivo gene expression, we suggest that cellular cocultures may offer enhanced in vitro models for nanoparticle risk assessment and the reduction of in vivo toxicological testing. (cdc.gov)

Autologous Endometrial Coculture is a technique of assisted reproductive technology. (wikipedia.org)
This study examines the effect of TGFbeta on extravillous cytotrophoblastic cell (EVCT) migration in cocultures of first-trimester human chorionic villus explants and primary human endometrial fibroblasts. (cnrs.fr)

Fibroblast cocultures with A818-6 cells were set up using foreskin-derived KIF-5 fibroblasts. (nih.gov)
Microstructured cocultures of cardiac myocytes and fibroblasts: a two-dimensional in vitro model of cardiac tissue. (surrey.ac.uk)
We used microfluidic techniques to grow two-dimensional structured cardiac tissue models, containing both myocytes and fibroblasts, and characterized cell morphology, distribution, and coupling using immunohistochemical techniques. (surrey.ac.uk)
We conclude that structured cocultures of cardiomyocytes and fibroblasts mimic in vivo ventricular tissue organization and provide a novel tool for in vitro research into cardiac electromechanical function. (surrey.ac.uk)

During coculture M. haemolytica growth was inhibited when B. trehalosi entered stationary phase (6 h) resulting in a final cell density for M. haemolytica that was 6 to 9 logs lower than expected with growth in the absence of B. trehalosi . (pubmedcentralcanada.ca)
Coculture supernatant failed to inhibit M. haemolytica growth on agar or in broth, indicating no obvious involvement of lytic phages, bacteriocins, or quorum-sensing systems. (pubmedcentralcanada.ca)
In addition, to gain a better understanding on the origin of different growth factors, both direct and indirect coculture strategies were employed. (hindawi.com)
To better mimic the lower growth fraction observed clinically, LNCaP human prostate cancer cells were cocultured with membrane-separated hFOB human osteoblasts. (elsevier.com)
Using RNase protection assays, it was documented that coculture with osteoblasts causes enhanced p53, p27, and p21 expression leading to a decrease in the number of LNCaP cells entering the cell cycle (i.e., enhanced number of LNCaP cells in G 0 -G 1 and a decrease in S and G 2 -M and thus the growth fraction). (elsevier.com)
This study is the first one to successfully use modern chemical-analytic techniques to visualize the spatial distribution of metabolites and in this way explain the growth of a bacterial colony consisting of cooperating bacteria. (phys.org)

Knockdown of MLC in OPC-DRG cocultures resulted in a significant increase in myelination similar to that observed in cultures treated with blebbistatin. (nih.gov)

While single-cell models of pulmonary exposure are often used to determine the potential toxicity of nanomaterials in vitro, previous studies have shown that coculture cell models better represent the cellular response and crosstalk that occurs in vivo. (cdc.gov)
Four sections explore in vitro techniques, in vivo and ex vivo manipulations, imaging and histological analysis and other novel techniques in vascular biology. (springer.com)

Therefore, techniques for the accurate fabrication of multifunctional scaffolds are needed. (biomedcentral.com)

To investigate human fibroblast proliferation and interleukin secretory profiles in response to modeled repetitive motion strain (RMS) and modeled indirect osteopathic manipulative techniques (IOMT). (jaoa.org)
Data from the present study suggest that fibroblast proliferation and expression/secretion of proinflammatory and anti-inflammatory interleukins may contribute to the clinical efficacy of indirect osteopathic manipulative techniques. (jaoa.org)
Indirect and direct co-culture techniques were used. (arvojournals.org)

Coculture of human prostate or bone stromal cells with human PCa LNCaP (LN) cells under three-dimensional conditions induced permanent genetic, morphologic, and behavioral changes in LN cells ( 4 ). (aacrjournals.org)
Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB), which may be human pathogens. (cdc.gov)
Non-autologous cell lines should be screened for infectious diseases prior to use in human embryo coculture. (advancedfertility.com)
Suturing techniques, as well as the usage of human amniotic membrane "bandages" were evaluated. (diva-portal.org)
The purpose of this project is to utilize whole genome sequencing analysis and human iPS cell-based approaches to develop technique(s) for the discovery of retinitis pigmentosa-causing gene mutations that escape standard methods of detection. (wisc.edu)
Three-dimensional human coronary in vitro models of leukocyte attack (3DLA models) focus on leukocyte adhesion and chemotaxis and the triggered proliferative response of cocultured SMCs. (ahajournals.org)

We observed that N-cadherin concentrated in the membrane of cocultured HBMSCs (co-HBMSCs) while distributed within the cytoplasm of monocultured HBMSCs, which indicated that the cell-cell adhesion was improved between cocultured cells. (nih.gov)
Coculture with agonists or antagonists of the membrane or nuclear vitamin D receptors indicated that these effects were primarily mediated by the nuclear vitamin D receptors. (jimmunol.org)
In this study, we show by an in vitro coculture system that GFP-tagged H-Ras, but not GFP, transfers from MEL526 to T cells and localizes to the inner aspect of their plasma membrane. (jimmunol.org)

Thus, amoebal coculture allows the recovery of new bacterial species from heavily contaminated samples and might be a valuable approach for the recovery of as-yet unrecognized emerging pathogens from clinical specimens. (cdc.gov)
Clinical studies have supported the efficacy of a variety of osteopathic manipulative techniques. (jaoa.org)
Current clinical culture techniques use an a priori decision on which organisms are likely to be present/important as causative infectious agents. (bmj.com)
There are currently no whole body imaging techniques in clinical use that are capable of specifically identifying bacterial infections. (spie.org)

Tissue engineering is considered to be the intracellular regulation by gene transduction and the extracellular regulation by culture methods, such as coculture. (osti.gov)
Novel biomaterials, artificial organ systems, cellular therapy and useful materials were investigated for utilizing the regulation techniques of cell proliferation. (osti.gov)

Dehalococcoides mccartyi 195 (strain 195) and Syntrophomonas wolfei were grown in a sustainable syntrophic coculture using butyrate as an electron donor and carbon source and trichloroethene (TCE) as an electron acceptor. (asm.org)
The upgrade would rely on a technique known as "co-culturing," in which different types of cells are grown together. (scientificamerican.com)
Here we used several metabolomic approaches to examine the chemicals made by these bacteria when grown in pairwise coculture. (asm.org)

With recent improvements in IVF culture media and techniques, our IVF pregnancy rates (without coculture) have improved dramatically. (advancedfertility.com)
They believe that this can yield an equally high pregnancy rate without the use of coculture. (advancedfertility.com)

Analysis of the cell cycle-associated proteins cyclin B and p27/Kip after coculture revealed a downregulation of p27/Kip and a weak upregulation of cyclin B in A818-6 hollow spheres (Fig. 4 B, lane 3). (nih.gov)

Results showed that cocultured cells expressed much higher N-cadherin than monocultured cells after 24 and 48 h of culture. (nih.gov)

Building upon previous expertise, this project will employ a combination of microfabrication and surface functionalisation techniques to create an electroanalytical microdevice with spatially controlled vascular cell populations. (strath.ac.uk)
The project will provide the opportunity to develop expertise in a wide range of both engineering and cell biology techniques, including microfabrication and lab-on-a-chip techniques, electrochemical sensors, vascular cell isolation and culture, and live-cell imaging. (strath.ac.uk)
Recent microfabrication techniques, such as hydrogel modeling has facilitated the development of vascular network models in vitro. (aiche.org)

Amoebal coculture could therefore be used to selectively grow these amoebae-resisting bacteria (ARB) and clean the samples of other more rapidly growing species that generally overwhelm the agar plates. (cdc.gov)
Limitations in standard culture (overgrowth by other bacteria/resilience of microorganisms to standard culture techniques) mean standard culture may not detect the diversity of bacteria present in non-cystic fibrosis bronchiectasis. (bmj.com)

Additionally, understanding tooth formation can lead to advances in dentistry, leading to improved restoration techniques. (tufts.edu)

The use of kefir coculture as a starter led to increased lactic acid concentrations and decreased pH values in the final product associated with significantly higher conversion rates compared to salted rennet cheese. (asm.org)

Stereolithography and fused filament fabrication techniques enable the 3D modeling of cancer cell metastasis to bone tissue. (corning.com)

The use of cell cultures to test the biocompatibility of drugs, biomaterials or treatment techniques used in various disciplines is gaining in importance. (egms.de)

Coculture involves a lot of tedious work in the laboratory which leads to additional expense. (advancedfertility.com)

Assisted Hatching (AH) is another commonly applied technique aimed at increasing implantation rates among infertile couples. (wikipedia.org)

These models are a further development of the transfilter coculture model that was first described by Betz and colleagues. (ahajournals.org)

Carbon monoxide in the coculture was around 0.06 μmol per bottle, which was lower than that observed for strain 195 in isolation. (asm.org)
High-throughput enrichments were set up with the MicroDrop technique using an alpine calcareous soil sample and a mixture of polymeric carbon compounds supplemented with signal compounds. (nih.gov)

Knockdown of myosin II in OL-DRG cocultures enhances myelin formation. (nih.gov)
To further investigate the contribution of OL myosin II on myelin formation, we infected OPC-DRG cocultures with shRNA lentivirus against the regulatory chain of MLC. (nih.gov)
This protocol describes a method for simultaneous imaging of thalamocortical axon branching and synapse formation in organotypic cocultures of the thalamus and cerebral cortex. (jove.com)
Matsumoto, N., Yamamoto, N. Visualization of Thalamocortical Axon Branching and Synapse Formation in Organotypic Cocultures. (jove.com)

An outstanding collection of detailed, state-of-the-art techniques for studying many aspects of nervous system cell biology. (indigo.ca)
These powerful techniques will illuminate nervous system function, injury, degeneration, and the repair/regenerative process. (indigo.ca)

While complete regeneration of corneal tissues occurred, only slight differences in effects of surgical technique could be found. (diva-portal.org)

However, the mechanisms by which ES cells are committed to the neural lineage using this technique are poorly understood. (biologists.org)

Nano3D Biosciences and MD Anderson have developed a magnetic 3D bioprinting technique to successfully print spheroids and contracting ring structures that model wound healing. (prweb.com)
As an initial step toward understanding these chemical interactions at a systems level, we employed the emerging techniques of nanospray desorption electrospray ionization (NanoDESI) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) imaging mass spectrometry to gain a global chemical view of the model bacterium Streptomyces coelicolor interacting with five other actinomycetes. (asm.org)

The book gives a thorough overview of applications of the LbL technique in the context of bioengineering and biomedical engineering where the last years have witnessed tremendous progress. (wiley.com)

Ann alternative for the control of virus diseases is the development of resistant plants through genetic transformation techniques. (usp.br)

For their study the scientists combined different methods and techniques. (innovations-report.com)

Anatomy19

Organisms3

Diseases1

Chemicals and Drugs8

Analytical, Diagnostic and Therapeutic Techniques and Equipment9

Phenomena and Processes12

Disciplines and Occupations2

Technology, Industry, Agriculture1

Geographicals1

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (1/7795)

Regulation of neurotrophin-3 expression by epithelial-mesenchymal interactions: the role of Wnt factors. (2/7795)

Reciprocal control of T helper cell and dendritic cell differentiation. (3/7795)

Endothelial cells modulate the proliferation of mural cell precursors via platelet-derived growth factor-BB and heterotypic cell contact. (4/7795)

Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis. (5/7795)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (6/7795)

In vitro hematopoietic and endothelial cell development from cells expressing TEK receptor in murine aorta-gonad-mesonephros region. (7/7795)

Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8(+) T cells resulting in a failure to lyse tumor cells. (8/7795)

Coculture Techniques

Cell Communication

Cells, Cultured

Stromal Cells

Culture Media, Conditioned

Paracrine Communication

Cell Differentiation

Fibroblasts

Cell Culture Techniques

Epithelial Cells

Bromobenzoates

Bone Marrow Cells

Cell Line

Hypodermoclysis

Endothelium, Vascular

Endothelial Cells

Tissue Engineering

Monocytes

Flow Cytometry

T-Lymphocytes

Mesenchymal Stromal Cells

Dendritic Cells

Cell Proliferation

Lymphocyte Activation

RNA, Messenger

Cytokines

Mice, Inbred C57BL

Culture Media

Cell Division

Acanthamoeba

Reverse Transcriptase Polymerase Chain Reaction

Macrophages

Fallopian Tubes

Cell Survival

Cell Adhesion

Signal Transduction

Hematopoietic Stem Cells

Cell Line, Tumor

Stem Cells

Antigens, CD

Interleukin-6

Apoptosis

Methanobacteriaceae

Cell Movement

Tumor Necrosis Factor-alpha

Spinal Cord Injuries

Spinal Cord

Neurosurgery

Nerve Regeneration

Hematology

Superior Sagittal Sinus

Germany

Systemic administration of rIL-12 synergistically enhances the therapeutic effect of a TNF gene-transduced cancer vaccine. (1/7795)

Regulation of neurotrophin-3 expression by epithelial-mesenchymal interactions: the role of Wnt factors. (2/7795)

Reciprocal control of T helper cell and dendritic cell differentiation. (3/7795)

Endothelial cells modulate the proliferation of mural cell precursors via platelet-derived growth factor-BB and heterotypic cell contact. (4/7795)

Establishment and characterization of nurse cell-like stromal cell lines from synovial tissues of patients with rheumatoid arthritis. (5/7795)

Microvessels from Alzheimer's disease brains kill neurons in vitro. (6/7795)

In vitro hematopoietic and endothelial cell development from cells expressing TEK receptor in murine aorta-gonad-mesonephros region. (7/7795)

Interleukin-10-treated human dendritic cells induce a melanoma-antigen-specific anergy in CD8(+) T cells resulting in a failure to lyse tumor cells. (8/7795)

Homeostatic T cell proliferation in a T cell-dendritic cell coculture system<...

Single-Cell RNA Sequencing Findings Encourage the Application of Three-Dimensional in Vitro Cell Co-Culture to Breast Cancer...

cell-cell contact in transwell systems: post #1

Asian Science Citation Index - Articles written by S. M Sheehan

95 Investigating fibroblast involvement in vascular inflammation using co-culture models | Heart

Collagen-based co-culture for invasive study on cancer cells-fibroblasts interaction<...

Cancer-associated fibroblasts and tumor growth--bystanders turning into key players

Induction of tumor and viral immunity using antigen presenting cell co-culture products and fusion products - University of...

Plus it

Sigma1 Receptors

A simplified culture system to examine soluble factor interactions between mammalian cells

Together with the ubiquitous expression of HSF1 and HSP90 while

Trumps win pushed this manufacturer to return to the U.S. - Dec. 7, 2016

Endothelial vascular smooth muscle cell coculture assay for high throu | IJHTS

NIOSHTIC-2 Publications Search - 20043891 - Differential gene expression in SAEC and HMVEC grown in monoculture or coculture...

Tumor-derived exosomes induce CD8+ T cell suppressors | Journal for ImmunoTherapy of Cancer | Full Text

High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model | February 18,...

High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model | February 18,...

High Throughput, Multiplexed Detection of Inflammatory Cytokines in an Astrocyte and Monocyte Co-culture Model | February 18,...

salmonella typhimurium Protocols and Video...