Staphylococcal Protein A
Bacterial Typing Techniques
Reagent Kits, Diagnostic
Microbial Sensitivity Tests
Electrophoresis, Gel, Pulsed-Field
Polymorphism, Restriction Fragment Length
Epidemiological characterization of methicillin-resistant Staphylococcus aureus isolated in the North West of England by protein A (spa) and coagulase (coa) gene polymorphisms. (1/806)In a comparative study, isolates of methicillin-resistant Staphylococcus aureus (MRSA) with known pulsed-field gel electrophoresis (PFGE) and bacteriophage type were analysed by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) for additional discriminatory subtyping information. PFGE was previously performed using standardized, commercially available kits and pre-programmed software. Isolates were examined for coagulase (coa) and protein A (spa) gene polymorphisms following PCR amplification of the coa hypervariable and spa repeat regions. Coa gene RFLPs produced a total of 38 distinct combined patterns after digestion with HaeIII and AluI and identified the predominant epidemic (EMRSA) types 15 and 16. A unique HaeIII restriction site was identified by RFLP and sequence analysis in the coa gene for EMRSA 15 but not EMRSA 16. The spa gene PCR yielded a total of 14 different profiles ranging from 3-18 repeats with the 2 predominant EMRSA types falling into 2 distinct groups. PCR detection of coa and spa polymorphisms offer a rapid preliminary strain identification and discriminatory subtyping information for surveillance of MRSA. (+info)
Changing susceptibilities of coagulase-negative staphylococci to teicoplanin in a teaching hospital. (2/806)The susceptibility of two collections of coagulase-negative staphylococci (CNS) isolated from clinical specimens for teicoplanin and vancomycin were compared. They comprised 91 and 101 isolates, collected in 1985 and 1994 respectively, from different departments of a teaching hospital. MICs of vancomycin and teicoplanin were determined by a modified Etest method. Additionally, a disc diffusion test was performed for teicoplanin. All isolates were susceptible to vancomycin (MIC < or = 4 mg/L). Two of the 91 isolates collected in 1985 were intermediate to teicoplanin (MIC between 8 and 32 mg/L), whereas in 1994 the number of intermediate isolates was 20 out of 101 (P < 0.01). The correlation between MICs, as determined by the modified Etest assay, and disc diffusion zones was poor (r = -0.35). Results show that resistance to teicoplanin in CNS has increased in the study hospital over a period of 9 years. This increase is likely to be correlated with the introduction of teicoplanin. Furthermore, a disc diffusion method does not appear to be the first method of choice for detection of strains of CNS with diminished susceptibility to teicoplanin. (+info)
Rapid identification of Staphylococcus aureus by using fluorescent staphylocoagulase assays. (3/806)Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-beta-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB). The assays were compared to the tube coagulase test and latex agglutination (LA) (Sanofi Diagnostics Pasteur, Guildford, Surrey, United Kingdom) by using 406 clinical isolates of staphylococci, and they produced positive and negative predictive values of 99.2 and 99. 1% for LA, 98.9 and 92.7% for VPA, and 98.9 and 99.1% for BB. Fluorescent assays used colonies from solid media, thereby eliminating the need for broth cultures, and were performed in microtiter trays, thus making them suitable for large-scale screening. (+info)
The Staphylococcus aureus rsbW (orf159) gene encodes an anti-sigma factor of SigB. (4/806)SigB, a newly discovered alternative sigma factor of Staphylococcus aureus, has been shown to play an important role in stress responses and the regulation of virulence factors. The rsbW (orf159) gene is immediately upstream of sigB. Its gene product is homologous to Bacillus subtilis RsbW which under appropriate conditions binds to B. subtilis SigB and functions as an anti-sigma factor or negative posttranslational regulator. To define the function of S. aureus RsbW, both the S. aureus SigB and RsbW proteins were expressed in Escherichia coli and purified. Cross-linking experiments with these purified proteins revealed that RsbW was capable of specific binding to SigB. In an in vitro transcription runoff assay, RsbW prevented SigB-directed transcription from the sar P3 promoter, a known SigB-dependent promoter, and the inhibitory activity of RsbW was found to be concentration dependent. We also identified SigB promoter consensus sequences upstream of the genes encoding alkaline shock protein 23 and coagulase and have demonstrated SigB and RsbW dependence for the promoters in vitro. These results show that RsbW is a protein sequestering anti-sigma factor of S. aureus SigB and suggest that SigB activity in S. aureus is regulated posttranslationally. (+info)
Use of semi-quantitative and quantitative culture methods and typing for studying the epidemiology of central venous catheter-related infections in neonates on parenteral nutrition. (5/806)To study the epidemiology - especially the impact of contaminated stopcocks - on central venous catheter (CVC) infection and catheter-related sepsis (CRS), semi-quantitative (SQ) and quantitative (Q) culture methods and typing of coagulase-negative staphylococci (CNS) were employed in 49 neonates with clinical signs of sepsis while receiving parenteral nutrition in the paediatric intensive care unit. The patients were divided into two groups according to stopcock contamination: group A consisted of 18 patients (36%) with contaminated stopcocks and group B consisted of 31 patients (64%) with sterile stopcocks. Five specimens were obtained from each patient, in addition to that from the stopcock: a swab taken from the skin surrounding the catheter puncture site; the CVC tip; the intradermal segment (IDC); and samples of parenteral fluid and blood. A total of 294 specimens (392 sites) was cultured and micro-organisms were identified. All CNS isolated were typed by biotyping, antibiogram, plasmid analysis and pulsed-field gel electrophoresis (PFGE), and the discriminatory power of the typing methods was compared. The CVC tips were infected in 25 patients (51%); 15 (83%) in group A and 10 (32%) in group B. Sepsis was detected in 24 neonates (49%), 13 in group A and 11 in group B. This was catheter-related in 15 patients (63%), 12 in group A and 3 in group B. CNS were recovered from 13 (52%) of 25 infected CVCs, nine in group A and four in group B. Sixty-five CNS isolates were recovered from these patients and belonged to 14 biotypes, 22 antibiograms, 22 plasmid profiles and 26 PFGE types. Typing showed that in six of nine patients in group A, CNS of the same type were recovered from the catheter tip and the stopcock, in one patient the catheter tip and skin isolates were the same and in two others the catheter tip isolates were different from stopcock and skin isolates. In all four patients in group B, different CNS types were recovered from CVC tips and skin. Bacteraemia was caused by CNS in 14 patients (58%), six in group A and eight in group B. Typing confirmed that nine cases (six in group A and three in group B) were catheter-related but five were not. SQ and Q culture methods and typing, especially by PFGE, allowed the study to determine that bacteria from contaminated stopcocks were frequently the source of CVC infection and CRS. (+info)
Oxacillin susceptibility testing of staphylococci directly from Bactec Plus blood cultures by the BBL Crystal MRSA ID system. (6/806)The BBL Crystal MRSA ID test (Becton Dickinson) was applied directly to blood culture vials containing clusters of gram-positive cocci. The sensitivity and specificity of the test were 84 and 100% and 54 and 100% for vials containing Staphylococcus aureus and coagulase-negative staphylococci, respectively. This test is a reliable method for direct detection of methicillin resistance in positive blood culture vials when S. aureus is identified in parallel by rapid identification procedures. (+info)
Analysis of genomic diversity within the Xr-region of the protein A gene in clinical isolates of Staphylococcus aureus. (7/806)Protein A of Staphylococcus aureus contains a polymorphic Xr-region characterized by a tandem repeat of eight amino acid units. In this study, the diversity of genes encoding the repeat regions and their relatedness among S. aureus strains was analyzed. Ten different protein-A types characterized by repeat numbers 4-13 were identified in a total of 293 clinical isolates. The protein-A type with 10 repeat units (10 repeats) in the Xr-region was most frequently detected in methicillin-resistant S. aureus, whereas the majority of methicillin-susceptible strains were distributed almost evenly into protein-A types with 7-11 repeats. Strains that belonged to a single coagulase type were classified into multiple protein-A types, e.g. strains with the common coagulase types II and VII were differentiated into 7 and 8 protein-A types, respectively. Nucleotide sequence analysis of the Xr-region of 42 representative strains revealed the presence of 37 different genotypes (spa types), which were constituted by a combination of several of 24 different repeat unit genotypes. Based on the similarity in arrangement of repeat unit genotypes, 34 strains with different repeat numbers were classified into 5 genetic clusters (C1-C5). The clusters C1, C2 and C3 consisted exclusively of strains with identical coagulase types II, III, and IV, respectively. These findings suggested that the protein-A gene of S. aureus has evolved from a common ancestral clone in individual clusters independently. (+info)
Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas. (8/806)The objectives of this study were to investigate the coagulase gene polymorphism of Staphylococcus aureus isolates obtained from bovine mastitic milk and to determine the resistance of predominant and rare coagulase genotypes to bovine blood neutrophil bactericidal activities. A total of 453 isolates were collected from four countries: the Czech Republic, France, Korea and the United States. The isolates were subtyped into 40 types by restriction fragment length polymorphism (RFLP) of the coagulase gene. Twenty-three strains from predominant and rare genotypes were evaluated for their ability to resist neutrophil bactericidal activities. There were significant (P < 0.01) differences in the average percent neutrophil killing of the predominant (16.7%) and rare (39.7%) genotypes when bacteria were opsonized with antiserum. The results indicate that the profiles of coagulase genotype differ among geographic locations, and only a few genotypes prevail in each location. In addition, the predominant genotypes were more resistant to neutrophil bactericidal activities than rare genotypes. (+info)
Staphylococcal infections can be classified into two categories:
1. Methicillin-Resistant Staphylococcus Aureus (MRSA) - This type of infection is resistant to many antibiotics and can cause severe skin infections, pneumonia, bloodstream infections and surgical site infections.
2. Methicillin-Sensitive Staphylococcus Aureus (MSSA) - This type of infection is not resistant to antibiotics and can cause milder skin infections, respiratory tract infections, sinusitis and food poisoning.
Staphylococcal infections are caused by the Staphylococcus bacteria which can enter the body through various means such as:
1. Skin cuts or open wounds
2. Respiratory tract infections
3. Contaminated food and water
4. Healthcare-associated infections
5. Surgical site infections
Symptoms of Staphylococcal infections may vary depending on the type of infection and severity, but they can include:
1. Skin redness and swelling
2. Increased pain or tenderness
3. Warmth or redness in the affected area
4. Pus or discharge
5. Fever and chills
6. Swollen lymph nodes
7. Shortness of breath
Diagnosis of Staphylococcal infections is based on physical examination, medical history, laboratory tests such as blood cultures, and imaging studies such as X-rays or CT scans.
Treatment of Staphylococcal infections depends on the type of infection and severity, but may include:
1. Antibiotics to fight the infection
2. Drainage of abscesses or pus collection
3. Wound care and debridement
4. Supportive care such as intravenous fluids, oxygen therapy, and pain management
5. Surgical intervention in severe cases.
Preventive measures for Staphylococcal infections include:
1. Good hand hygiene practices
2. Proper cleaning and disinfection of surfaces and equipment
3. Avoiding close contact with people who have Staphylococcal infections
4. Covering wounds and open sores
5. Proper sterilization and disinfection of medical equipment.
It is important to note that MRSA (methicillin-resistant Staphylococcus aureus) is a type of Staphylococcal infection that is resistant to many antibiotics, and can be difficult to treat. Therefore, early diagnosis and aggressive treatment are crucial to prevent complications and improve outcomes.
Staphylococcal food poisoning is typically caused by consuming food that has been contaminated with the bacterium, often through improper handling or storage of food products. Symptoms usually begin within 1-7 days after consumption of contaminated food, and can last for several days to a week or more.
Treatment for staphylococcal food poisoning typically involves supportive care, such as hydration and rest, as well as antibiotics in severe cases. In severe cases, hospitalization may be necessary to manage complications such as kidney failure or sepsis. Prevention measures include proper handling and storage of food products, cooking food to the appropriate temperature, and avoiding cross-contamination of foods.
The most common symptoms of bovine mastitis include:
1. Swelling and redness of the udder
2. Increased temperature of the affected quarter
3. Pain or discomfort in the udder
4. Decreased milk production
5. Abnormal milk appearance (e.g., clots, pus, or blood)
If left untreated, mastitis can lead to more severe complications such as abscesses, septicemia, or even death of the animal. Therefore, early detection and proper treatment are crucial to prevent the spread of infection and reduce the severity of clinical signs.
Diagnosis of bovine mastitis is typically based on a combination of clinical examination, milk culture, and laboratory tests (e.g., blood counts, serum biochemistry). Treatment involves antibiotics, supportive care (e.g., fluids, pain management), and identification of the underlying cause to prevent future occurrences.
Prevention of bovine mastitis is key to maintaining a healthy and productive dairy herd. This includes proper sanitation and hygiene practices, regular milking techniques, and effective dry cow therapy. Vaccination against common mastitis-causing pathogens may also be considered in some cases.
Overall, bovine mastitis is a significant health issue in dairy cattle that can have significant economic and welfare implications for farmers and the industry as a whole. Proper diagnosis, treatment, and prevention strategies are essential to maintain a healthy and productive herd.
In medicine, cross-infection refers to the transmission of an infectious agent from one individual or source to another, often through direct contact or indirect exposure. This type of transmission can occur in various settings, such as hospitals, clinics, and long-term care facilities, where patients with compromised immune systems are more susceptible to infection.
Cross-infection can occur through a variety of means, including:
1. Person-to-person contact: Direct contact with an infected individual, such as touching, hugging, or shaking hands.
2. Contaminated surfaces and objects: Touching contaminated surfaces or objects that have been touched by an infected individual, such as doorknobs, furniture, or medical equipment.
3. Airborne transmission: Inhaling droplets or aerosolized particles that contain the infectious agent, such as during coughing or sneezing.
4. Contaminated food and water: Consuming food or drinks that have been handled by an infected individual or contaminated with the infectious agent.
5. Insect vectors: Mosquitoes, ticks, or other insects can transmit infections through their bites.
Cross-infection is a significant concern in healthcare settings, as it can lead to outbreaks of nosocomial infections (infections acquired in hospitals) and can spread rapidly among patients, healthcare workers, and visitors. To prevent cross-infection, healthcare providers use strict infection control measures, such as wearing personal protective equipment (PPE), thoroughly cleaning and disinfecting surfaces, and implementing isolation precautions for infected individuals.
In summary, cross-infection refers to the transmission of an infectious agent from one individual or source to another, often through direct contact or indirect exposure in healthcare settings. Preventing cross-infection is essential to maintaining a safe and healthy environment for patients, healthcare workers, and visitors.
Bacteremia can occur when bacteria enter the bloodstream through various means, such as:
* Infected wounds or surgical sites
* Injecting drug use
* Skin infections
* Respiratory tract infections
* Urinary tract infections
* Endocarditis (infection of the heart valves)
The symptoms of bacteremia can vary depending on the type of bacteria and the severity of the infection. Some common symptoms include:
* Muscle aches
* Shortness of breath
Bacteremia is diagnosed by blood cultures, which involve collecting blood samples and inserting them into a specialized container to grow the bacteria. Treatment typically involves antibiotics and supportive care, such as intravenous fluids and oxygen therapy. In severe cases, hospitalization may be necessary to monitor and treat the infection.
Prevention measures for bacteremia include:
* Practicing good hygiene, such as washing hands regularly
* Avoiding sharing personal items like toothbrushes or razors
* Properly cleaning and covering wounds
* Getting vaccinated against infections that can lead to bacteremia
* Following proper sterilization techniques during medical procedures
Overall, bacteremia is a serious condition that requires prompt medical attention to prevent complications and ensure effective treatment.
Symptoms of mastitis may include:
* Pain and tenderness in the breast
* Redness and swelling of the skin
* Fever and chills
* Difficulty breathing (in severe cases)
Treatment for mastitis usually involves antibiotics to clear up any bacterial infections, as well as measures to relieve pain and discomfort such as warm compresses, massage, and over-the-counter pain medication. In some cases, hospitalization may be necessary if the infection is severe or if the person has a fever that is difficult to control.
Preventative measures for mastitis include:
* Properly storing and handling breast milk
* Frequently checking and emptying the breasts to prevent clogged milk ducts
* Avoiding tight clothing or bras that can constrict the breasts
* Practicing good hygiene, such as washing hands before handling the breasts
It is important for individuals with mastitis to seek medical attention if they experience any of the following symptoms:
* High fever (over 101°F)
* Difficulty breathing
* Severe pain that does not improve with medication
* Redness or swelling that spreads beyond the breast area.
Staphylococcal pneumonia is an infection of the lungs that occurs when the bacteria enter the lungs and cause inflammation. This condition can be particularly dangerous for certain groups of people, such as children under the age of two, adults over the age of 65, and those with weakened immune systems or chronic medical conditions.
The symptoms of staphylococcal pneumonia can vary depending on the severity of the infection, but they may include:
* Fever and chills
* Cough, which may be dry or produce mucus
* Chest pain that worsens with deep breathing or coughing
* Shortness of breath
* Muscle aches and fatigue
* Confusion or disorientation in older adults
Staphylococcal pneumonia is typically diagnosed through a combination of physical examination, medical history, and diagnostic tests such as chest X-rays or blood tests. Treatment typically involves antibiotics, which can help clear the infection and reduce symptoms. In severe cases, hospitalization may be necessary to provide supportive care, such as oxygen therapy or mechanical ventilation.
Prevention of staphylococcal pneumonia is key, and this includes:
* Vaccination: The pneumococcal conjugate vaccine (PCV) is recommended for children under the age of two and adults over the age of 65, as well as those with certain medical conditions.
* Good hygiene practices: Washing hands regularly, covering the mouth and nose when coughing or sneezing, and avoiding close contact with people who are sick can help prevent the spread of the bacteria.
* Avoiding risky behaviors: Avoiding sharing personal items, such as toothbrushes or drinking glasses, and not engaging in risky behaviors like unprotected sex or sharing needles can also help prevent the spread of the bacteria.
It is important to seek medical attention if you or a loved one is experiencing symptoms of pneumonia, as early treatment can help prevent complications and improve outcomes.
Antibiotic sensitivity testing
Clumping factor A
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Coagulase Mannitol Agar 1lb | P212121 Store
Comparison of methods for the detection of biofilm production in coagulase-negative staphylococci | BMC Research Notes | Full...
Staphylococcus (coagulase-negative methicillin-susceptible) | The Antimicrobial Index Knowledgebase - TOKU-E
Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight...
Phenotypic and genotypic characterisation of linezolid-resistant coagulase-negative staphylococci possessing cfr-carrying...
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- Are coagulase-negative Staphylococcus (CoNS) species important? (cdc.gov)
- Coagulase Mannitol Agar is used for the differentiation of Staphylococcus aureus from other species based on coagulase production and mannitol utilization. (p212121.com)
- Staphylococci, both coagulase-positive and coagulase-negative Staphylococcus species (CoNS), have major medical significance. (p212121.com)
- In contrast, a coagulase-negative species that does not utilize mannitol, such as Staphylococcus epidermidis, does not change the color of the medium and it remains clear. (p212121.com)
- Sur 60 hémocultures positives pour les cocci à Gram positif en grappes, l'amplification isotherme induite par boucle (au moyen du dépistage des gènes FemA et MecA) a montré une sensibilité et une spécificité de 100 % pour l'identification de Staphylococcus aureus résistant et sensible à la méthicilline. (who.int)
- To analyse the concentration of serum level in the voucher (one hour before the next administration of the drug dose) of vancomycin in newborns with Staphylococcus aureus infection or oxacillin-resistant coagulase-negative. (bvsalud.org)
- We selected 30 patients who had staphylococcus aureus and coagulase-negative sepsis and used vancomycin as a treatment. (bvsalud.org)
- both coagulase-negative Staphylococcus and gram-negative rods grew in 1 culture. (cdc.gov)
- The most common pathogens were coagulase-negative Staphylococcus (CoNS), MSSA, and enterococci. (medscape.com)
- Staphylococcus epidermidis and other coagulase-negative staphylococci. (medlineplus.gov)
- 1. Staphylococcus lugdunensis: a rare but destructive cause of coagulase-negative staphylococcus infective endocarditis. (nih.gov)
- 6. Unusually virulent coagulase-negative Staphylococcus lugdunensis is frequently associated with infective endocarditis: a Waikato series of patients. (nih.gov)
- 11. Coagulase-negative Staphylococcus endocarditis: Staphylococcus lugdunensis. (nih.gov)
- 17. The pitfall of coagulase-negative staphylococci: a case of Staphylococcus lugdunensis endocarditis. (nih.gov)
- The Pathogen Molecular Genetics section investigates physiology and host interaction of Gram-positive pathogens with a focus on Staphylococcus aureus and coagulase-negative staphylococci. (nih.gov)
- K. pneumoniae, P. mirabilis, coagulase-neg Staphylococcus spp. (medscape.com)
- Staphylococcus aureus (3 patients) and staphylococcus coagulase negative (3 patients) were the most common infectious agents during direct connection period. (isciii.es)
- 1987. Emergence of vancomycin resistance in coagulase-negative staphylococci. (cdc.gov)
- 1 Coagulase-producing staphylococci (S. aureus) may be differentiated and presumptively identified with this medium based on production of coagulase and mannitol utilization. (p212121.com)
- Coagulase Mannitol Agar aids in the differentiation of staphylococci by indicating the presence of coagulase and the utilization of mannitol. (p212121.com)
- The ability of biofilm formation seems to play an essential role in the virulence of coagulase-negative staphylococci (CNS). (biomedcentral.com)
- Biofilm production was studied in 80 coagulase-negative staphylococci (CNS) strains isolated from clinical specimens of newborns with infection hospitalized at the Neonatal Unit of the University Hospital, Faculty of Medicine of Botucatu, and in 20 isolates obtained from the nares of healthy individuals without signs of infection. (biomedcentral.com)
- Coagulase-negative staphylococci (CNS) are the microorganisms most frequently involved in nosocomial infections among neonates. (biomedcentral.com)
- Coagulase-Negative Staphylococci Contained in Gut Microbiota as a Primary Source of Sepsis in Low- and Very Low Birth Weight Neonates. (bvsalud.org)
- There are only a few reports in the literature about translocation of coagulase -negative staphylococci (CoNS) as a primary cause of sepsis in neonates , although CoNS are among a short list of "translocating" bacteria when present in abundance. (bvsalud.org)
- Phenotypic and genotypic characterisation of linezolid-resistant coagulase-negative staphylococci possessing cfr-carrying plasmid. (illumina.com)
- This suggests that tigecycline may have also been useful for the coagulase-negative staphylococci identified in this patient and could have been considered as the sole treatment agent. (cdc.gov)
- The remaining six isolates were coagulase-negative staphylococci. (bl.uk)
- The assay can distinguish coagulase-negative Staphylococci from pathogenic Staphylococci , the company said. (genomeweb.com)
- 8. [Infective endocarditis caused by coagulase-negative staphylococci: a review of 19 cases]. (nih.gov)
- Contamination rates varied from 10% to 100%, with coagulase-negative staphylococci being the most commonly found microorganism, being present on 441 of the tourniquets analyzed. (medindia.net)
- Coagulase-negative staphylococci can cause a range of infections, including skin and soft tissue infections. (medindia.net)
- Gordon is interested in the molecular mechanisms underlying the pathogenic success of S. aureus and coagulase-negative staphylococci. (nih.gov)
- Higher prevalence of coagulase-negative staphylococci carriage among reclaimed water spray irrigators. (cdc.gov)
- Cardiovascular infections are primarily caused by coagulase positive and negative staphylococci and by other antimicrobial-drug resistant pathogens. (nih.gov)
- Two coagulase-negative staphylococci emerging as potential zoonotic pathogens: wolves in sheep's clothing? (nih.gov)
- Listeria and coagulase-positive Staphylococci have been found during inspections of fruit salads in Switzerland, according to the Swiss Association of Cantonal Chemists (ACCS). (foodsafetynews.com)
- DCDD along with direct tube coagulase assay detected only 80.6% of MRSA/MSSA. (who.int)
- A tube coagulase test using rabbit plasma with EDTA was then performed on Staphaurex-negative isolates from BAP with morphology consistent with S. aureus and Staphaurex-positive isolates with morphology inconsistent with S. aureus (non-hemolytic). (cdc.gov)
- Staphaurex-positive isolates and Staphaurex-negative tube coagulase-positive isolates were identified as S. aureus and saved for further testing. (cdc.gov)
- Staphaurex-positive tube coagulase-negative isolates were discarded. (cdc.gov)
- NOTE: The use of BBL Coagulase Plasma, Rabbit with EDTA, in place of citrated plasma, prevents false-positive coagulase reactions by citrate-utilizing microorganisms. (p212121.com)
- and Marwin introduced tellurite-glycine media designed to selectively isolate coagulase-positive staphylococcal species. (p212121.com)
- 4. Cool to 50°C and add 7-15% pretested, undiluted rabbit coagulase plasma with EDTA. (p212121.com)
- S. schleiferi (coagulase positive or negative) was viewed as an uncommon pathogen of dogs and humans. (vin.com)
- Coagulase production is dependant on the presence of mannitol, a protein factor in the brain heart infusion and blood serum (plasma). (p212121.com)
- An opaque area of coagulated plasma forms around the colonies of organisms that also produce coagulase. (p212121.com)
- En parallèle, une identification microbiologique normalisée et un test de sensibilité à l'oxacilline par PCR visant l'amplification du gène MecA ont été réalisés. (who.int)