Coagulase: Enzymes that cause coagulation in plasma by forming a complex with human PROTHROMBIN. Coagulases are produced by certain STAPHYLOCOCCUS and YERSINIA PESTIS. Staphylococci produce two types of coagulase: Staphylocoagulase, a free coagulase that produces true clotting of plasma, and Staphylococcal clumping factor, a bound coagulase in the cell wall that induces clumping of cells in the presence of fibrinogen.Staphylococcus: A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.Staphylococcus aureus: Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.Staphylococcal Infections: Infections with bacteria of the genus STAPHYLOCOCCUS.Staphylococcal Protein A: A protein present in the cell wall of most Staphylococcus aureus strains. The protein selectively binds to the Fc region of human normal and myeloma-derived IMMUNOGLOBULIN G. It elicits antibody activity and may cause hypersensitivity reactions due to histamine release; has also been used as cell surface antigen marker and in the clinical assessment of B lymphocyte function.Bacteriological Techniques: Techniques used in studying bacteria.Methicillin Resistance: Non-susceptibility of a microbe to the action of METHICILLIN, a semi-synthetic penicillin derivative.Mannitol: A diuretic and renal diagnostic aid related to sorbitol. It has little significant energy value as it is largely eliminated from the body before any metabolism can take place. It can be used to treat oliguria associated with kidney failure or other manifestations of inadequate renal function and has been used for determination of glomerular filtration rate. Mannitol is also commonly used as a research tool in cell biological studies, usually to control osmolarity.Staphylococcal Food Poisoning: Poisoning by staphylococcal toxins present in contaminated food.Mastitis, Bovine: INFLAMMATION of the UDDER in cows.Agar: A complex sulfated polymer of galactose units, extracted from Gelidium cartilagineum, Gracilaria confervoides, and related red algae. It is used as a gel in the preparation of solid culture media for microorganisms, as a bulk laxative, in making emulsions, and as a supporting medium for immunodiffusion and immunoelectrophoresis.Tolonium Chloride: A phenothiazine that has been used as a hemostatic, a biological stain, and a dye for wool and silk. Tolonium chloride has also been used as a diagnostic aid for oral and gastric neoplasms and in the identification of the parathyroid gland in thyroid surgery.Synsepalum: A plant genus of the family SAPOTACEAE. Members contain miraculin (also spelled mieraculin), a sweet protein.Phenolphthaleins: A family of 3,3-bis(p-hydroxyphenyl)phthalides. They are used as CATHARTICS, indicators, and COLORING AGENTS.Bacterial Typing Techniques: Procedures for identifying types and strains of bacteria. The most frequently employed typing systems are BACTERIOPHAGE TYPING and SEROTYPING as well as bacteriocin typing and biotyping.Staphylococcus Phages: Viruses whose host is Staphylococcus.Oxacillin: An antibiotic similar to FLUCLOXACILLIN used in resistant staphylococci infections.Micrococcaceae: A family of bacteria ranging from free living and saprophytic to parasitic and pathogenic forms.Deoxyribonucleases: Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-.Bacteriophage Typing: A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.Equipment Contamination: The presence of an infectious agent on instruments, prostheses, or other inanimate articles.Staphylococcus lugdunensis: A species of gram-positive bacteria in the family STAPHYLOCOCCACEAE. It is responsible for skin and soft-tissue infections among others, and is part of the normal human skin flora.Staphylococcus haemolyticus: A species of STAPHYLOCOCCUS found on the skin of humans (and non-human primates), often causing hospital-acquired infections (CROSS INFECTION).Cross Infection: Any infection which a patient contracts in a health-care institution.Staphylococcus epidermidis: A species of STAPHYLOCOCCUS that is a spherical, non-motile, gram-positive, chemoorganotrophic, facultative anaerobe. Mainly found on the skin and mucous membrane of warm-blooded animals, it can be primary pathogen or secondary invader.Bacteremia: The presence of viable bacteria circulating in the blood. Fever, chills, tachycardia, and tachypnea are common acute manifestations of bacteremia. The majority of cases are seen in already hospitalized patients, most of whom have underlying diseases or procedures which render their bloodstreams susceptible to invasion.Mastitis: INFLAMMATION of the BREAST, or MAMMARY GLAND.Preservation, Biological: The process of protecting various samples of biological material.Anti-Bacterial Agents: Substances that reduce the growth or reproduction of BACTERIA.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Micrococcal Nuclease: An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1.Agglutination Tests: Tests that are dependent on the clumping of cells, microorganisms, or particles when mixed with specific antiserum. (From Stedman, 26th ed)Microbial Sensitivity Tests: Any tests that demonstrate the relative efficacy of different chemotherapeutic agents against specific microorganisms (i.e., bacteria, fungi, viruses).Milk: The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.Blood: The body fluid that circulates in the vascular system (BLOOD VESSELS). Whole blood includes PLASMA and BLOOD CELLS.Pneumonia, Staphylococcal: Pneumonia caused by infections with bacteria of the genus STAPHYLOCOCCUS, usually with STAPHYLOCOCCUS AUREUS.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Lysostaphin: A 25-kDa peptidase produced by Staphylococcus simulans which cleaves a glycine-glcyine bond unique to an inter-peptide cross-bridge of the STAPHYLOCOCCUS AUREUS cell wall. EC 3.4.24.75.Hydrolases: Any member of the class of enzymes that catalyze the cleavage of the substrate and the addition of water to the resulting molecules, e.g., ESTERASES, glycosidases (GLYCOSIDE HYDROLASES), lipases, NUCLEOTIDASES, peptidases (PEPTIDE HYDROLASES), and phosphatases (PHOSPHORIC MONOESTER HYDROLASES). EC 3.Hemolysin Proteins: Proteins from BACTERIA and FUNGI that are soluble enough to be secreted to target ERYTHROCYTES and insert into the membrane to form beta-barrel pores. Biosynthesis may be regulated by HEMOLYSIN FACTORS.Electrophoresis, Gel, Pulsed-Field: Gel electrophoresis in which the direction of the electric field is changed periodically. This technique is similar to other electrophoretic methods normally used to separate double-stranded DNA molecules ranging in size up to tens of thousands of base-pairs. However, by alternating the electric field direction one is able to separate DNA molecules up to several million base-pairs in length.Food Microbiology: The presence of bacteria, viruses, and fungi in food and food products. This term is not restricted to pathogenic organisms: the presence of various non-pathogenic bacteria and fungi in cheeses and wines, for example, is included in this concept.Polymorphism, Restriction Fragment Length: Variation occurring within a species in the presence or length of DNA fragment generated by a specific endonuclease at a specific site in the genome. Such variations are generated by mutations that create or abolish recognition sites for these enzymes or change the length of the fragment.Encyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Fibrin: A protein derived from FIBRINOGEN in the presence of THROMBIN, which forms part of the blood clot.Yersinia pestis: The etiologic agent of PLAGUE in man, rats, ground squirrels, and other rodents.Phagocytosis: The engulfing and degradation of microorganisms; other cells that are dead, dying, or pathogenic; and foreign particles by phagocytic cells (PHAGOCYTES).beta 2-Glycoprotein I: A 44-kDa highly glycosylated plasma protein that binds phospholipids including CARDIOLIPIN; APOLIPOPROTEIN E RECEPTOR; membrane phospholipids, and other anionic phospholipid-containing moieties. It plays a role in coagulation and apoptotic processes. Formerly known as apolipoprotein H, it is an autoantigen in patients with ANTIPHOSPHOLIPID ANTIBODIES.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Antibody Specificity: The property of antibodies which enables them to react with some ANTIGENIC DETERMINANTS and not with others. Specificity is dependent on chemical composition, physical forces, and molecular structure at the binding site.Vancomycin: Antibacterial obtained from Streptomyces orientalis. It is a glycopeptide related to RISTOCETIN that inhibits bacterial cell wall assembly and is toxic to kidneys and the inner ear.Catheterization, Central Venous: Placement of an intravenous CATHETER in the subclavian, jugular, or other central vein.British Columbia: A province of Canada on the Pacific coast. Its capital is Victoria. The name given in 1858 derives from the Columbia River which was named by the American captain Robert Gray for his ship Columbia which in turn was named for Columbus. (From Webster's New Geographical Dictionary, 1988, p178 & Room, Brewer's Dictionary of Names, 1992, p81-2)Catheters, Indwelling: Catheters designed to be left within an organ or passage for an extended period of time.

Epidemiological characterization of methicillin-resistant Staphylococcus aureus isolated in the North West of England by protein A (spa) and coagulase (coa) gene polymorphisms. (1/806)

In a comparative study, isolates of methicillin-resistant Staphylococcus aureus (MRSA) with known pulsed-field gel electrophoresis (PFGE) and bacteriophage type were analysed by polymerase chain reaction (PCR) and restriction fragment length polymorphisms (RFLP) for additional discriminatory subtyping information. PFGE was previously performed using standardized, commercially available kits and pre-programmed software. Isolates were examined for coagulase (coa) and protein A (spa) gene polymorphisms following PCR amplification of the coa hypervariable and spa repeat regions. Coa gene RFLPs produced a total of 38 distinct combined patterns after digestion with HaeIII and AluI and identified the predominant epidemic (EMRSA) types 15 and 16. A unique HaeIII restriction site was identified by RFLP and sequence analysis in the coa gene for EMRSA 15 but not EMRSA 16. The spa gene PCR yielded a total of 14 different profiles ranging from 3-18 repeats with the 2 predominant EMRSA types falling into 2 distinct groups. PCR detection of coa and spa polymorphisms offer a rapid preliminary strain identification and discriminatory subtyping information for surveillance of MRSA.  (+info)

Changing susceptibilities of coagulase-negative staphylococci to teicoplanin in a teaching hospital. (2/806)

The susceptibility of two collections of coagulase-negative staphylococci (CNS) isolated from clinical specimens for teicoplanin and vancomycin were compared. They comprised 91 and 101 isolates, collected in 1985 and 1994 respectively, from different departments of a teaching hospital. MICs of vancomycin and teicoplanin were determined by a modified Etest method. Additionally, a disc diffusion test was performed for teicoplanin. All isolates were susceptible to vancomycin (MIC < or = 4 mg/L). Two of the 91 isolates collected in 1985 were intermediate to teicoplanin (MIC between 8 and 32 mg/L), whereas in 1994 the number of intermediate isolates was 20 out of 101 (P < 0.01). The correlation between MICs, as determined by the modified Etest assay, and disc diffusion zones was poor (r = -0.35). Results show that resistance to teicoplanin in CNS has increased in the study hospital over a period of 9 years. This increase is likely to be correlated with the introduction of teicoplanin. Furthermore, a disc diffusion method does not appear to be the first method of choice for detection of strains of CNS with diminished susceptibility to teicoplanin.  (+info)

Rapid identification of Staphylococcus aureus by using fluorescent staphylocoagulase assays. (3/806)

Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-beta-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB). The assays were compared to the tube coagulase test and latex agglutination (LA) (Sanofi Diagnostics Pasteur, Guildford, Surrey, United Kingdom) by using 406 clinical isolates of staphylococci, and they produced positive and negative predictive values of 99.2 and 99. 1% for LA, 98.9 and 92.7% for VPA, and 98.9 and 99.1% for BB. Fluorescent assays used colonies from solid media, thereby eliminating the need for broth cultures, and were performed in microtiter trays, thus making them suitable for large-scale screening.  (+info)

The Staphylococcus aureus rsbW (orf159) gene encodes an anti-sigma factor of SigB. (4/806)

SigB, a newly discovered alternative sigma factor of Staphylococcus aureus, has been shown to play an important role in stress responses and the regulation of virulence factors. The rsbW (orf159) gene is immediately upstream of sigB. Its gene product is homologous to Bacillus subtilis RsbW which under appropriate conditions binds to B. subtilis SigB and functions as an anti-sigma factor or negative posttranslational regulator. To define the function of S. aureus RsbW, both the S. aureus SigB and RsbW proteins were expressed in Escherichia coli and purified. Cross-linking experiments with these purified proteins revealed that RsbW was capable of specific binding to SigB. In an in vitro transcription runoff assay, RsbW prevented SigB-directed transcription from the sar P3 promoter, a known SigB-dependent promoter, and the inhibitory activity of RsbW was found to be concentration dependent. We also identified SigB promoter consensus sequences upstream of the genes encoding alkaline shock protein 23 and coagulase and have demonstrated SigB and RsbW dependence for the promoters in vitro. These results show that RsbW is a protein sequestering anti-sigma factor of S. aureus SigB and suggest that SigB activity in S. aureus is regulated posttranslationally.  (+info)

Use of semi-quantitative and quantitative culture methods and typing for studying the epidemiology of central venous catheter-related infections in neonates on parenteral nutrition. (5/806)

To study the epidemiology - especially the impact of contaminated stopcocks - on central venous catheter (CVC) infection and catheter-related sepsis (CRS), semi-quantitative (SQ) and quantitative (Q) culture methods and typing of coagulase-negative staphylococci (CNS) were employed in 49 neonates with clinical signs of sepsis while receiving parenteral nutrition in the paediatric intensive care unit. The patients were divided into two groups according to stopcock contamination: group A consisted of 18 patients (36%) with contaminated stopcocks and group B consisted of 31 patients (64%) with sterile stopcocks. Five specimens were obtained from each patient, in addition to that from the stopcock: a swab taken from the skin surrounding the catheter puncture site; the CVC tip; the intradermal segment (IDC); and samples of parenteral fluid and blood. A total of 294 specimens (392 sites) was cultured and micro-organisms were identified. All CNS isolated were typed by biotyping, antibiogram, plasmid analysis and pulsed-field gel electrophoresis (PFGE), and the discriminatory power of the typing methods was compared. The CVC tips were infected in 25 patients (51%); 15 (83%) in group A and 10 (32%) in group B. Sepsis was detected in 24 neonates (49%), 13 in group A and 11 in group B. This was catheter-related in 15 patients (63%), 12 in group A and 3 in group B. CNS were recovered from 13 (52%) of 25 infected CVCs, nine in group A and four in group B. Sixty-five CNS isolates were recovered from these patients and belonged to 14 biotypes, 22 antibiograms, 22 plasmid profiles and 26 PFGE types. Typing showed that in six of nine patients in group A, CNS of the same type were recovered from the catheter tip and the stopcock, in one patient the catheter tip and skin isolates were the same and in two others the catheter tip isolates were different from stopcock and skin isolates. In all four patients in group B, different CNS types were recovered from CVC tips and skin. Bacteraemia was caused by CNS in 14 patients (58%), six in group A and eight in group B. Typing confirmed that nine cases (six in group A and three in group B) were catheter-related but five were not. SQ and Q culture methods and typing, especially by PFGE, allowed the study to determine that bacteria from contaminated stopcocks were frequently the source of CVC infection and CRS.  (+info)

Oxacillin susceptibility testing of staphylococci directly from Bactec Plus blood cultures by the BBL Crystal MRSA ID system. (6/806)

The BBL Crystal MRSA ID test (Becton Dickinson) was applied directly to blood culture vials containing clusters of gram-positive cocci. The sensitivity and specificity of the test were 84 and 100% and 54 and 100% for vials containing Staphylococcus aureus and coagulase-negative staphylococci, respectively. This test is a reliable method for direct detection of methicillin resistance in positive blood culture vials when S. aureus is identified in parallel by rapid identification procedures.  (+info)

Analysis of genomic diversity within the Xr-region of the protein A gene in clinical isolates of Staphylococcus aureus. (7/806)

Protein A of Staphylococcus aureus contains a polymorphic Xr-region characterized by a tandem repeat of eight amino acid units. In this study, the diversity of genes encoding the repeat regions and their relatedness among S. aureus strains was analyzed. Ten different protein-A types characterized by repeat numbers 4-13 were identified in a total of 293 clinical isolates. The protein-A type with 10 repeat units (10 repeats) in the Xr-region was most frequently detected in methicillin-resistant S. aureus, whereas the majority of methicillin-susceptible strains were distributed almost evenly into protein-A types with 7-11 repeats. Strains that belonged to a single coagulase type were classified into multiple protein-A types, e.g. strains with the common coagulase types II and VII were differentiated into 7 and 8 protein-A types, respectively. Nucleotide sequence analysis of the Xr-region of 42 representative strains revealed the presence of 37 different genotypes (spa types), which were constituted by a combination of several of 24 different repeat unit genotypes. Based on the similarity in arrangement of repeat unit genotypes, 34 strains with different repeat numbers were classified into 5 genetic clusters (C1-C5). The clusters C1, C2 and C3 consisted exclusively of strains with identical coagulase types II, III, and IV, respectively. These findings suggested that the protein-A gene of S. aureus has evolved from a common ancestral clone in individual clusters independently.  (+info)

Coagulase gene polymorphism of Staphylococcus aureus isolates from dairy cattle in different geographical areas. (8/806)

The objectives of this study were to investigate the coagulase gene polymorphism of Staphylococcus aureus isolates obtained from bovine mastitic milk and to determine the resistance of predominant and rare coagulase genotypes to bovine blood neutrophil bactericidal activities. A total of 453 isolates were collected from four countries: the Czech Republic, France, Korea and the United States. The isolates were subtyped into 40 types by restriction fragment length polymorphism (RFLP) of the coagulase gene. Twenty-three strains from predominant and rare genotypes were evaluated for their ability to resist neutrophil bactericidal activities. There were significant (P < 0.01) differences in the average percent neutrophil killing of the predominant (16.7%) and rare (39.7%) genotypes when bacteria were opsonized with antiserum. The results indicate that the profiles of coagulase genotype differ among geographic locations, and only a few genotypes prevail in each location. In addition, the predominant genotypes were more resistant to neutrophil bactericidal activities than rare genotypes.  (+info)

PURPOSE: To evaluate the fluoroquinolone susceptibilities of ocular isolate coagulase-negative staphylococci (CoNS), identified at the Microbiology Laboratory - UNIFESP. DESIGN: Experimental laboratory investigation. METHODS: The minimum inhibitory concentrations (MICs) of 21 strains of methicillin-resistant coagulase-negative staphylococci (MRCoNS) and 22 methicillin-sensitive coagulase-negative staphylococci (MSCoNS) to ciprofloxacin, ofloxacin, gatifloxacin and moxifloxacin were determined, using the E-test method standardized by the Clinical and Laboratory Standards Institute (CLSI/NCCLS). RESULTS: The MIC90s (µg/ml) for the second generation of tested fluoroquinolones were higher than the fourth generation, especially for the methicillin-resistant coagulase-negative staphylococci group. CONCLUSION: Our results indicate that methicillin-sensitive coagulase-negative staphylococci are more susceptible to quinolones than are methicillin-resistant coagulase-negative staphylococci and that ...
References for Abcams Anti-Staphylococcus aureus coagulase Type I antibody (ab79636). Please let us know if you have used this product in your publication
The pathogenic role of staphylococcal coagulase and clumping factor was investigated in the rat model of endocarditis. The coagulase-producing and clumping factor-producing parent strain Staphylococcus aureus Newman and a series of mutants defective in either coagulase, clumping factor, or both were tested for their ability (i) to attach in vitro to either rat fibrinogen or platelet-fibrin clots and (ii) to produce endocarditis in rats with catheter-induced aortic vegetations. In vitro, the clumping factor-defective mutants were up to 100 times less able than the wild type strain to attach to fibrinogen and also significantly less adherent than the parents to platelet-fibrin clots. Coagulase-defective mutants, in contrast, were not altered in their in vitro adherence phenotype. The rate of in vivo infection was inoculum dependent. Clumping factor-defective mutants produced ca. 50% less endocarditis than the parent organisms when injected at inoculum sizes infecting, respectively, 40 and 80% ...
Antimicrobial resistance patterns and gene coding for methicillin resistance (mecA) were determined in 25 S. aureus and 75 Coagulase Negative Staphylococci (CNS) strains isolates from half-udder milk samples collected from goats with subclinical mastitis. Fourteen (56.0%) S. aureus and thirty-one (41.3%) CNS isolates were resistant to one or more antimicrobial agents. S. aureus showed the highest resistance rate against kanamycin (28.0%), oxytetracycline (16.0%), and ampicillin (12.0%). The CNS tested were more frequently resistant to ampicillin (36.0%) and kanamycin (6.7%). Multiple antimicrobial resistance was observed in eight isolates, and one Staphylococcus epidermidis was found to be resistant to six antibiotics. The mecA gene was not found in any of the tested isolates. Single resistance against β-lactamics or aminoglicosides is the most common trait observed while multiresistance is less frequent. ...
Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is generally coagulase-positive, meaning that coagulase negativity usually excludes S. aureus. However it is now known that not all S. aureus are coagulase-positive. It is also produced by Yersinia pestis. Coagulase reacts with prothrombin in the blood. The resulting complex is called staphylothrombin, which enables the enzyme protease to convert fibrinogen, a plasma protein produced by the liver, to fibrin. This results in clotting of the blood. Coagulase is tightly bound to the surface of the bacterium S. aureus and can coat its surface with fibrin upon contact with blood. The fibrin clot may protect the bacterium from phagocytosis and isolate it from other defenses of the host.The fibrin coat can therefore make the bacteria more virulent. Bound coagulase is ...
Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. In the laboratory, it is used to distinguish between different types of Staphylococcus isolates. Importantly, S. aureus is coagulase-positive, meaning that coagulase negativity excludes S. aureus.. It is also produced by Yersinia pestis.. Coagulase reacts with prothrombin in the blood. The resulting complex is called staphylothrombin, which enables the enzyme protease to convert fibrinogen to fibrin. This results in clotting of the blood. Coagulase is tightly bound to the surface of the bacterium S. aureus and can coat its surface with fibrin upon contact with blood. It has been proposed that fibrin-coated staphylococci resist phagocytosis, making the bacteria more virulent. Bound coagulase is part of the larger family of MSCRAMM. ...
Author: Surekha.Y.Asangi , Mariraj.J , Sathyanarayan.M.S , Nagabhushan , Rashmi :: Coagulase Negative Staphylococci (cons) Are The Indigenous Flora Of The Human Skin And Mucous Membrane. They Are Usually Contaminants, When Isolated From A Clinical Specimen. These Organisms Are Becoming Increasingly Recognized As Agents Of Clinically...
BACKGROUND: Antimicrobial resistance may compromise the efficacy of antibiotic prophylaxis before surgery. The aim of this study was to measure susceptibility and clonal distribution of coagulase-negative staphylococci (CoNS) colonizing the skin around the surgery access site before and after the procedure. METHODS: From March to September 2004, a series of 140 patients undergoing elective major abdominal surgery were screened for CoNS colonization at admission and 5 days after surgery. All isolates were tested for antibiotic susceptibility and genotyped by pulsed-field gel electrophoresis (PFGE). RESULTS: Colonization rates with CoNS at admission and after surgery were 85% and 55%, respectively. The methicillin-resistant CoNS rate increased from 20% at admission to 47% after surgery (P = 0.001). The PFGE pattern after surgery revealed more patients colonized with identical clones: 8/140 patients (8/119 strains) and 26/140 patients (26/77 strains), respectively (P , 0.001). CONCLUSIONS: Our ...
Coagulase-negative staphylococci (CoNS) are a major cause of nosocomial blood stream infection, especially in critically ill and haematology patients. CoNS are usually multidrug-resistant and glycopeptide antibiotics have been to date considered the drugs of choice for treatment. The aim of this study was to characterize CoNS with reduced susceptibility to glycopeptides causing blood stream infection (BSI) in critically ill and haematology patients at the University Hospital Tor Vergata, Rome, Italy, in 2007. Hospital microbiology records for transplant haematology and ICU were reviewed to identify CoNS with elevated MICs for glycopeptides, and isolates were matched to clinical records to determine whether the isolates caused a BSI. The isolates were tested for susceptibility to new drugs daptomicin and tigecycline and the genetic relationship was assessed using f-AFLP. Of a total of 17,418 blood cultures, 1,609 were positive for CoNS and of these, 87 (5.4%) displayed reduced susceptibility to
Legionella species are generally susceptible to fluroquinolones or macrolides "Small-colony variant" Staphylococcus aureus (SCV) These are a form of Staphylococcus aureus. They are not recognised on routine culture media as they grow slowly. They also have a delayed coagulase positive test which can be delayed up to 24 hours. The common (or the wild type) S aureus compared takes just 4 hours to exhibit coagulase positivity. For this reason, these are commonly identified as coagulase-negative staphylococci. The best way to recognise them is to incubate for at least 3 days and look for pinpoint colonies, and then to await the coagulase positive test. These microbes have a reduced metabolic capacity and are therefore less pathogenic. However, it can persist intracellularly and cause chronic infection, and are resistant to routine antibiotics (which are effective against the wild type). They are very common in patients who have received or are on antibiotics, especially aminoglycosides. SSIs caused ...
One of the most important phenotypical features used in the classification of staphylococci is their ability to produce coagulase, an enzyme that causes blood clot formation. Seven species are currently recognised as being coagulase-positive: S. aureus, S. delphini, S. hyicus, S. intermedius, S. lutrae, S. pseudintermedius, and S. schleiferi subsp. coagulans. These species belong to two separate groups - the S. aureus (S. aureus alone) group and the S. hyicus-intermedius group (the remaining five). An eighth species has also been described - Staphylococcus leei - from patients with gastritis.[17]. S. aureus is coagulase-positive, meaning it produces coagulase. However, while the majority of S. aureus strains are coagulase-positive, some may be atypical in that they do not produce coagulase. S. aureus is catalase-positive (meaning that it can produce the enzyme catalase) and able to convert hydrogen peroxide (H2O2) to water and oxygen, which makes the catalase test useful to distinguish ...
Hello Eric, Streptococcus genus contains both beta-hemolytic and alpha hemolytic species. S. pyogenes and S.agalactiae are beta-hemolytic but S.pneumoniae and viridans streptococci (common name for various Streptococci species that gives alpha hemolysis) are alpha hemolytic. In Staphylococci, S.aureus is the pathogenic one but S. epidermidis, S. saprophyticus are mostly commensal (normal flora) and occasionally be pathogenic. So common phrase Coagulase Negative Staphylococci (CONS) is used for them; (S. aureus is coagulase positive).. ...
From a total of 40 characters that were previously used to differentiate species of staphylococci, 13 key characters were selected to make a simplified scheme that could be easily used by the routine clinical laboratory for identifying human staphylococci. These key characters included coagulase activity, hemolysis, nitrate reduction, and aerobic acid production from fructose, xylose, arabinose, ribose, maltose, lactose, sucrose, trehalose, mannitol, and xylitol. In the simplified scheme, 924 strains of staphylococci were placed into 11 positions, each of which contained the major portion (greater than or equal to 80%) of strains of one of the recognized species. Several positions contained a rare or few uncommon strains of one or more additional species and these could be resolved on the basis of other key characters. ...
Results: The incidence of NIs was low (i.e. , 2%). Among 16936 admitted patients in this hospital, 174 patients (79 males and 95 females) with a mean age of 51.7 ± 24.6 years (range, 5 to 90 years) were diagnosed with an NI. Incidence density of NIs were 3.18% in infectious diseases ward, 2.17% in intensive care unit (ICU), 2% in orthopedic ward, 0.68% in obstetrics and gynecology (OBGYN) ward and 0.278% in general surgery. Regarding the etiology of infection, coagulase-negative staphylococci in 23.69%, Bacillus in 21.05%, Escherichia coli was found in 18.42%, and coagulase-positive staphylococci in 13.16% of the cases. The results indicated that coagulase-negative staphylococci was the most frequent pathogen. ...
Lyophilized rabbit plasma, for the identification of Staphylococcus aureus|/italic by the coagulase testDehydrated microbiology culture media cultivate and isolate microorganisms for researching purposes. Fast-dissolving powders come premixed for quick preparation using neutral liquids. By standardizing the media employed, the low-dust granule mixtures guarantee that the correct enrichment will be present for optimal cell viability and growth. The mixtures have extensive dry storage capabilities. The vast ingredient selection ensures that the most compatible dehydrated microbiology culture media will be available.
常見的臨床微生物簡介. 萬芳醫院 感染科 歐聰億. 格蘭氏染色 and 形狀. 格蘭氏陽性. 葡萄球菌- Staphylococcus Staphylococcus aureus Staphylococcus epidermidis Coagulase negative staphylococcus (CoNS). 鏈球菌 - streptococcus Streptococcus pneumoniae (pneumococcus) Viridans streptococcus (αhemolytyic)( 綠色) Slideshow 6870346 by lisandra-graham
Merlino, J., Kay, I., Coombs, G., Palladino, S. (2010). PCR Assays in Detecting Methicillin Resistance in Staphylococci: Coagulase Negative Staphylococci (CNS), S. Aureus, and MRSA with the PVL Gene. In Margaret Schuller, Theo Sloots, Gregory James, Catriona Halliday, Ian Carter (Eds.), PCR for Clinical Microbiology - An Australian and International Perspective, (pp. 407-414). Dordrecht: Springer Science+Business Media. [More Information] ...
Oliveira, Ad lia Dias Dourado et al. Laboratory detection methods for methicillin resistance in coagulase negative Staphylococcus isolated from ophthalmic infections. Arq. Bras. Oftalmol., Aug 2007, vol.70, no.4, p.667-675. ISSN 0004- ...
Staphylococcus is a gram positive, cocci shaped, genus of bacteria. Observed under a microscope will reveal they exist in microscopic grape-like clusters. One species of staphylococci, Staphylococcus aureus ...
Central venous catheter represents a major source of nosocomial bloodstream infection, which cause considerable excess morbidity.The diagnosis of catheter-related infections relies on the presence of clinical manifestation of infection and the evidence of colonization of the catheter tip by bacteria or fungi. The most frequent pathogens were Gram-positive organisms, mainly coagulase negative staphylococci, followed by Gram-negative and mycetes. During the years 2001-2003 we examined 2079 vascular catheters, of which 896 were positive for bacterial and fungal species. Of these, 675 (75,3%) involved Gram-positive bacterial, 145 (16,2%) Gram-negative and 76 (8,5%) mycetes ...
Manufacture and distribution of animal blood products, plasma, serum, microbiological diagnostic kits and quality control products, Buckinghamshire, UK
But Im a little different to him, Im called a "coagulase negative" staph, which means I react differently then him in a special test. ...
But Im a little different to him, Im called a "coagulase negative" staph, which means I react differently then him in a special test. ...
Skin microbiome primary cultivable aerobes in human are coagulase-negative staphylococci and lipophilic corynebacteria. in the research of human physiological flora which has been recently called microbiome (Turnbaugh et al. 2007). Natural flora plays an important role in a process of preventing colonization of the skin by pathogenic organisms known as colonization resistance of the skin. […]. Read More ». ...
Items covered in a dichotomous key include whether bacteria are Gram positive or negative, catalase positive or negative, have nitrate enzymes, and contain the enzyme coagulase. The key is a set of...
Find out if a staph infection is contagious, learn how a staph infection is transmitted, and discover when to seek medical care for a suspected staph infection.
When skin is punctured or broken for any reason, staph bacteria can enter the wound and cause an infection. But good hygiene can prevent many staph infections. Learn more.
When skin is punctured or broken for any reason, staph bacteria can enter the wound and cause an infection. But good hygiene can prevent many staph infections. Learn more.
The result of (we think) a staph infection. Doctor cut it open and drained a lot of blood and puss from it and then bandaged it up and put me on antib...
Coloured scanning electron micrograph (SEM) of Staphylococcus warneri, coccoid, Gram-positive prokaryote. Staphylococcus warneri is a common commensal bacterium found on the skin of humans and animals. It is a coagulase-negative Staphylococcus sp. that rarely causes disease but it may cause infection in immunocompromised patients. It occasionally has been associated with cases of septicaemia and bacteraemia. Staphylococcus warneri is sensitive to the antibiotic, vancomycin. Magnification: x6,000 when shortest axis printed at 25 millimetres. - Stock Image F017/4255
Define Staphylococcus capitis. Staphylococcus capitis synonyms, Staphylococcus capitis pronunciation, Staphylococcus capitis translation, English dictionary definition of Staphylococcus capitis. n. pl. staph·y·lo·coc·ci Any of various spherical gram-positive parasitic bacteria of the genus Staphylococcus that usually occur in grapelike clusters and...
Patients admitted into the Intensive Care Unit (ICU) have an intravenous (IV) catheter (small plastic tube) placed in their vein. Very occasionally (4 times out of 100) the insertion of an intravenous catheter may cause an infection in the blood. It has been shown that the removal of the catheter and the insertion of a new one at a new site helps to get rid of this infection. Sometimes, antibiotics are also given.. Vancomycin is the antibiotic given intravenously (into the vein) to treat these catheter-related infections. At Vancouver General Hospital, some physicians may not give any vancomycin at all whereas others may treat with intravenous (IV) vancomycin for one to fourteen days.. Since there are a lack of data to support the length of IV vancomycin therapy, the investigators would like to find out if two days of IV vancomycin are as good as seven days.. Therefore, the purpose of this study is to determine if two days of IV vancomycin are as good as seven days for the treatment of ...
The book covers updated topics on the genus Staphylococcus, including the latest discoveries. In each chapter, the author discusses the results obtained and published during her more than 20 years as a researcher in this area. The book is divided into eight chapters, with a presentation of the genus Staphylococcus, current classification, general characteristics of these bacteria and the clinical significance of coagulase-negative staphylococci (CoNS), which are often classified as mere blood culture contaminants, but are the etiological agents most commonly associated with neonatal infections and peritonitis in patients undergoing peritoneal dialysis. Coagulase-negative staphylococci can colonize the surface of catheters and produce an extracellular polysaccharide that leads to the formation of biofilms which potentiate their pathogenicity. The book also focuses on the identification of CoNS, virulence factors responsible for the symptoms and severity of infections caused by Staphylococcus spp. ...
Sharing More than Friendship - Nasal Colonization with Coagulase-Positive Staphylococci CPS and Co-Habitation Aspects of Dogs and Their Owners. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Staphylococcus warneri lipase 2: A gene encoding an extracellular lipase was identified in Staphylococcus warneri 863; amino acid sequence in first source
Results: The results showed 91 isolates (56.88%) were coagulase-positive, and 69 isolates (43.12%) were coagulase-negative Staphylococcus aureus (CNSA). Out of 91 (56.88%) coagulase-positive staphylococci, 32 isolates (35.16%) were resistant to cefoxitin, and 30 isolates (32.96%) were resistant to oxacillin, using disc diffusion method. PCR revealed the presence of the femA gene (510 bp band) in all coagulase-positive isolates (100%), and the mecA gene (513 bp band) was detected in 32 isolates (35.16%); out of 32 MRSA isolates, 13 isolates (40.62%) were positive for presence of the luk-pv gene (433 bp band). ...
We have shown that there are at least three distinct types of SCCmec in the chromosome of MRSA worldwide. SCCmec was defined as the DNA element on the MRSA chromosome demarcated by a pair of direct repeats and inverted repeats, havingccr genes required for its movement and carrying themecA gene (14, 17). As far as we could judge from the structure of the two elements newly identified in this study, they seem to constitute a family of SCCmec together with N315-type SCCmec.. The mecA gene is considered to have originated in some coagulase-negative staphylococcus species (36) and was then transferred into S. aureus to generate MRSA (1,13, 32). It is likely that SCCmec serves as the carrier of the mecA gene moving across staphylococcal species, since mecA genes in other staphylococcal species have never been found without the accompaniment of SCCmec-like structure (T. Ito and Y. Katayama, unpublished observation). Since both ccrA and ccrB genes are required for the integration event, we considered ...
Name: Staphylococcus warneri Kloos and Schleifer 1975 (Approved Lists 1980). Category: Species. Proposed as: sp. nov.. Etymology: N.L. gen. masc. n. warneri, of Warner, named for Arthur Warner, from whom this organism was originally isolated Type strains: ATCC 27836; CCUG 7325; CIP 81.65; DSM 20316; JCM 2415; LMG 13354; NCTC 11044; NRRL B-14736 See detailed strain information at ...
Staphylococci are the most abundant skin-colonizing bacteria and the most important causes of nosocomial infections and community-associated skin infections. and birds [1]. Two main groups are distinguished by their ability to coagulate blood: coagulase-positive staphylococci, with the most important species being or as a subject, owing to its eminent role in human contamination. The nose is the most important site PNU 282987 of colonization [3], but is situated in the pharynx also, perineum, axillae and on your skin (predominantly over the hands, upper body and abdomen) [4C6]. Consistent colonization with is normally observed in around 20% of the populace, while 30% bring transiently, and around 50% are non-carriers [7,8]. In consistent providers, who all possess within their noses, the regularity of colonization of various other body sites is normally increased weighed against the general people [9]. Consistent carriage prices are higher in kids than adults [4]. Oddly enough, theres been a ...
Seventy-three percent of coagulase negative Staphylococci (CoNS) Isolated from blood cultures were contaminants and 34% of patients with contaminating CoNS were treated with specific anti-staphylococcal antibiotics including vancomycin. This paper underlines the need for careful evaluation of CoNS from blood culture before instituting therapy ...
If you consider that MRSA causes meningitis, pneumonia and toxic shock syndrome and is scary, we have other nasty bacteria in our hospitals; E. Coli cases up by 48% in 2007; Clostridium difficile showing a 72% rise in 2007 with over 5,000 deaths attributed to it; Klebsiella pneumoniae 5,198 cases hitting and killing people who have underlying lung problems (causes abscesses in the lungs); Streptococcus pneumoniae 4,553 cases and 14,943 cases of Coagulase negative staphylococcus causing bloodstream infections. In the USA, C. difficile was responsible for 18,650 deaths last year and now exceeds AIDs deaths there. What are the real risks today in the UK concerning acquiring a serious hospital infection? High , is the probable answer.. The historical period of effective anti-biotics has perhaps now passed after 50 years and we need to re-institute simple Victorian inspired hygiene measures that lapsed because we had the anti-biotics to sort out problems caused by slack hygiene. Anyone who remembers ...
To introduce and demonstrate the principle and experimental set up for determining the microbes ability to detoxify hydrogen peroxide and /or to cause blood coagulation.
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Abstract. This study included collection of 100 specimens from patients in AL-Kindy Teaching Hospital and teaching laboratories of Medical City Hospitals in Baghdad during the period from August to December 2012 ,these specimens differed in their sources which included 19 nasal swab, 16 wound swab,27 burn swab, 7 pus, 15 sputum, 10 corneal swab and 6 urine . Only 38 (38%) isolates was identified as Staphylococcus. In this study, 29 isolates (76.3%) were coagulase-positive (COPS), while only 9 isolates(23.6%) were coagulase negative (CONS), from total 38 isolates of Staphylococci. The distribution of Methicillin resistance among Staphylococcus spp. was investigated by disc diffusion method. In this study, 21 isolates (55.26%) showed resistant to the Methicillin while 17 isolates (44.73%) were sensitive. The ability to produce slime layer by MRSA isolates was also investigated and the results showed that all isolates of MRSA was produced slime layer when tested by tube method, but the amounts of ...
Buy ISO 6888-1:1999/Amd 1:2003 Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of coagulase-positive staphylococci (Staphylococcus aureus and other species) Part 1: Technique using Baird-Parker agar medium - Amendment 1: Inclusion of precision data from SAI Global
Design: DNA was extracted using UltraClean microbial DNA isolation kit (Mo Bio). Shotgun sequencing libraries were prepared using Fragmentase (Epicentre BioTechnologies), standard Y-adaptor ligation, PCR amplification using Kapa HiFi (KAPA Biosystems), and were purified using Agencourt AMPure XP (Beckman Coulter). ...
This paper reports the results of a pilot study, which examines the respiratory control exerted by chest and abdominal-muscles… Expand ...
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Health care professionals can determine that its a staph infection by taking a culture (a swab with a giant Q-tip) from the infected site. Once staph is diagnosed, your provider prescribes antibiotics that kill that specific bacterial strain.. Hospitals are trying to stop staph infections, because most hospital patients are at risk, and because drug-resistant strains of staph (versions of the bacteria not vulnerable to the antibiotics used to treat staph infections) are becoming more common. People with resistant staph infections may require hospitalization to receive antibiotics through an IV or by injection.. As far as we know, however, bacteria do not develop resistance to the complex natural antibiotics in herbs. ...
How to Prevent Staph Infection. Staphylococcus aureus, or staph, is a very common type of bacteria. According to the Center for Disease Control and Prevention (the CDC), 30% of people have the staph bacteria in their noses, and 20% of...
I would imagine not. On the other hand, skin staphylococcus is often a cleanliness issue. If you get funky, then get a cut, the staph that lives on you 24-7 is more likely to become pathological than if youre clean. The majority of people carry staph around at some point in their life. Usually it doesnt cause an infection. So in that respect, people who get staph may be more likely to get it again if theyre just not keeping themselves real clean ...
3M™ Petrifilm™ Staph Express Count Plates provide Staphylococcus aureus results. S. aureus appears as distinctive red-violet colonies. Other colonies have to be checked using the Staph express disk.
Do you know what a staph infection is? Learn about the causes, symptoms, and treatment of staph infections (Staphylococcus aureus) before they turn dangerous.
HealthTap: Doctor answers on Symptoms, Diagnosis, Treatment, and More: Dr. Persaud on what is staph infection look like: Skin infections with staph can take many shapes. It could be flat redness or a pus filled boil or just a red bump.
Exactly what it says. Ive been sidelined for the past three weeks or so with staph. Did not respond to bactrin but did respond to an IV dose of, then prescrip of clindamycin. Currently about 2x2 inches on my left calf. If I were to wrap it up in three layers of bandaging then throw a pair of clean shinguards over it, would I be a risk to my training partners? Gi only, obviously. I did ask my doctor about this, but theyre not grapplers, so I dont think she understood the degree of contact
Watch out, infection. University of Iowa researchers have created a probe that can identify staph bacteria before symptoms appear. The probe is noninvasive and is expected to be cheaper and faster than current diagnostic techniques. Results published in the journal Nature Medicine.
TheBody.com fills you in on the topic, staph infection in the vaginal area, with a wealth of fact sheets, expert advice, community perspective, the latest news/research, and much more.
MetaFilter is a weblog that anyone can contribute a link or a comment to. A typical weblog is one person posting their thoughts on the unique things they find on the web. This website exists to break down the barriers between people, to extend a weblog beyond just one person, and to foster discussion among its members.. ...
S. lugdunensis is a recently described coagulase negative Staphylococcus species that has been determined by our group and others to be a virulent human pathogen, capable of causing diseases more akin to Staphylococcus aureus than a typical coagulase negative Staphylococcus species (e.g., native valve endocarditis). This suggests that this species has unique characteristics differentiating it from other coagulase negative Staphylococcus species. The types of infection caused by S. lugdunensis, supported by data generated in our laboratory demonstrating the ability of this organism to form biofilm, suggest that biofilm formation contributes to this species virulence.. We have identified a locus with homology to the S. aureus and Staphylococcus epidermidis ica loci in S. lugdunensis. Interestingly, S. lugdunensis forms biofilm, but its biofilm extracellular matrix is predominantly proteinaceous. Understanding the mechanisms of biofilm formation in S. lugdunensis should enable new, more effective ...
Deoxyribonucleic acid relatedness studies (S1 nuclease method) showed that 23 unidentified Staphylococcus strains form two homogeneous genomic species related 1 to 9% to 24 type strains representing known Staphylococcus species. These new species were named Staphylococcus lugdunensis and Staphylococcus schleiferi. Strains of S. lugdunensis were susceptible to novobiocin, produced a fibrinogen affinity factor, and failed to produce coagulase, heat-stable nuclease, and staphylokinase. S. lugdunensis strains differed from S. hominis (the phenotypically closest species) by production of ornithine decarboxylase and the fibrinogen affinity factor. The guanine-plus-cytosine content of the deoxyribonucleic acid was 32 mol%. The type strain is N860297 (= ATCC 43809). Strains of S. schleiferi were susceptible to novobiocin, produced a heat-stable nuclease and a fibrinogen affinity factor, and failed to produce coagulase and staphylokinase. S. schleiferi strains differed from S. aureus by production of an
Aim: This Swedish study determined which species of coagulase-negative staphylococci (CoNS) were found in neonatal blood cultures and whether they included Staphylococcus capitis clones with decreased susceptibility to vancomycin. Methods: CoNS isolates (n = 332) from neonatal blood cultures collected at orebro University Hospital during 1987-2014 were identified to species level with matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS). The antibiotic susceptibility pattern of S. capitis isolates was determined by the disc diffusion test and Etest, and the presence of heterogeneous glycopeptide-intermediate S. capitis (hGISC) was evaluated. Results: Staphylococcus epidermidis (67.4%), Staphylococcus haemolyticus (10.5%) and S. capitis (9.6%) were the most common CoNS species. Of the S. capitis isolates, 75% were methicillin-resistant and 44% were multidrug-resistant. No isolate showed decreased susceptibility to vancomycin, but at least 59% displayed the ...
Staphylococcus chromogenes is a Gram-positive, coagulase-negative member of the bacterial genus Staphylococcus consisting of clustered cocci. The species is associated with mastitis in dairy animals. Nickerson, SC (Feb 16, 2009). "Control of heifer mastitis: antimicrobial treatment-an overview". Veterinary microbiology. 134 (1-2): 128-35. doi:10.1016/j.vetmic.2008.09.019. PMID 18963599. Vanderhaeghen, W; Piepers, S; Leroy, F; Van Coillie, E; Haesebrouck, F; De Vliegher, S (June 18, 2014). "Invited review: Effect, persistence, and virulence of coagulase-negative Staphylococcus species associated with ruminant udder health". Journal of Dairy Science. 97 (9). doi:10.3168/jds.2013-7775. PMID 24952781. Type strain of Staphylococcus chromogenes at BacDive - the Bacterial Diversity ...
Results Most common blood culture isolates in decreasing order of frequency were Klebsiella (42.4%), Coagulase Negative Staphylococci (11.2%), Enterobacter (9.4%), Escherichia coli (9.1%), Pseudomonas (5.4%) and Acinetobacter (4.7%). Gram negative organisms were predominant in early and late onset neonatal sepsis as well as in inborn and outborn babies. Staphylococcus aureus and Enterococci were uncommon. Candida species were isolated in early onset sepsis and in babies weighing more than 1500 gm. Most gram negative organisms were resistant to ampicillin, gentamicin and cephalosporins. Sensitivity of amikacin, levofloxacin and piperacillin-tazobactam against Gram negative organisms ranged from 25% to 75%. Incidence of Methicillin Resistant Staphylococcus Aureus and Vancomycin resistant Enterococci was 33% and 20% respectively. Most Candida isolates were sensitive to antifungals. The most effective first line antibiotic combinations were amikacin with levofloxacin and amikacin with ...
L PARVARESH¹, S C-A CHEN, V W LEE³´⁴, V SINTCHENKO¹´²´⁴. ¹Centre for Infectious Diseases and Microbiology-Public Health, Westmead Hospital, Westmead, NSW; ²Centre for Infectious Diseases and Microbiology Laboratory Services, ICPMR, NSW Health Pathology, Westmead Hospital, NSW; ³Department of Renal Medicine, Westmead Hospital, Westmead, NSW; ⁴Sydney Medical School, The University of Sydney, NSW. Aim: This review of episodes of bacteraemia in haemodialysis patients, admitted to a tertiary hospital in Sydney and dialysed through any type of vascular access, was conducted in order to determine the frequency and patterns of antimicrobial resistance or causative organisms.. Background: Bacteraemia is the most common infection-related cause of mortality in patients with kidney disease on haemodialysis. Staphylococcus aureus, coagulase negative staphylococci (CONS), Pseudomonas aeruginosa, Escherichia coli, Klebsiella spp., and Enterobacter spp. are the most frequent isolates. Antibiotic ...
More specific detection methods in recent years have allowed further investigation of the coryneform bacterias.C macginleyi was first identified in 1995 by Riegel et al 5-7 during investigations on lipophilic corynebacteria. It has been uniquely isolated from ocular surfaces. The first 18 cases ofC macginleyi conjunctivitis have been detected in Switzerland.8 Within the recent past we found in 10 patients 13 cases of C macginleyiconjunctivitis in Germany, indicating that the presence of this micro-organism is not geographically limited.. Thiel et al report on increasing percentage of patients positive for corynebacteria.10 11 We found in our patients 18.7% Staphylococcus aureus, 12.1% Corynebacterium macginleyi, and 8.4%Haemophilus influenzae. Fahmiet al, however, found coagulase negative staphylococci in 82% and corynebacteria in 58% of their mainly elderly patients.12 In our study group we foundC macginleyi predominantly in middle aged patients without any preference regarding sex. This is in ...
Staphylococcus saprophyticus subsp. saprophyticus ATCC ® 15305™ Designation: NCTC 7292 TypeStrain=True Application: Quality control
An agr homologue of Staphylococcus saprophyticus was identified, cloned and sequenced. The gene locus shows homologies to other staphylococcal agr systems, especially to those of S. epidermidis and S. lugdunensis. A putative RNAIII was identified and found to be differentially expressed during the growth phases. In contrast to the RNAIII molecules of S. epidermidis and S. aureus it does not contain an open reading frame that codes for a protein with homologies to the d-toxin. Using PCR, the agr was found to be present in clinical isolates of S. saprophyticus. ...
Bloodstream infection is a common cause of morbidity in children aged ,5 years in developing countries. In studies reporting bacteremia in Africa, coagulase-negative Staphylococci (CoNS) are commonly isolated. However, it is currently unclear whether children who are highly susceptible to infection because of severe acute malnutrition (SAM) or HIV should be treated with antimicrobials specifically to cover CoNS. We aimed to determine the clinical significance of CoNS amongst children admitted to a rural hospital in Kenya in relation to nutritional and HIV status. Systematically collected clinical and microbiological surveillance data from children aged 6-59 months admitted to Kilifi County Hospital (2007-2013) were analysed. Multivariable regression was used to test associations between CoNS isolation from blood cultures and SAM (MUAC ,11.5cm or nutritional oedema (kwashiorkor)), and HIV serostatus; and among children with SAM or HIV, associations between CoNS isolation and mortality, duration ...
The culture grew out coagulase positive staph sensitive to everything; even Penicillin G. This is unusual in the U.S. where most Saph is resistant to penicillin. One wonders if this is a strain he brought over from Russia when he was adopted. I started him on Pen VK 250 mg qid. I will add a topical corticosteroid and mupirocin - the latter for nares and crural folds. Will give follow-up after a couple of weeks. If he continues to have staph infections like this, I will look into his Ig status. ...
MRSA is a yellow staphylococcus bacterium. The difference between other yellow staphylococci and MRSA is that MRSA is resistant to certain antibiotics. This means that if you get an infection, there are fewer antibiotics that can be used. Another word for resilience is resistance.. It is common to have bacteria like yellow staphylococci on the body without having any problems. Its called being a carrier. The staphylococci are usually present in the nose, throat or other mucous membranes. You can also wear them on the skin, for example in groin and armpits.. ...
Evaluation des enfants de r fugi s et d immigrants : r le du d pistage des parasites www.cps.ca/francais/enonces/ID/id94-07.htm. R sistance b talactamine. R sistance aux b talactamines www.biomath.jussieu.fr/bacterio/betalact/index.html. Lignes directrices pour l valuation de la sensibilit des Enterobacteriaceae r sistants aux antibiotiques cause des b-lactamases spectre tendu (BLSE) et le signalement des cas www.hc-sc.gc.ca/hpb/lcdc/bmb/ceqaagar/esb198_f.html. R sistance m thicilline. Le contr le du staphylocoque dor r sistant la m thicilline dans les tablissements p diatriques canadiens est toujours d actualit www.cps.ca/francais/enonces/ID/id99-03.htm. Lignes directrices pour l valuation de la sensibilit aux antimicrobiens de Staphylococcus aureus r sistant la m thicilline (SARM) et le signalement des cas et commentaires sur les staphylocoques coagulase n gative r sistants la m thicilline (SCN-RM). www.hc-sc.gc.ca/hpb/lcdc/bmb/ceqaagar/mrsa98_f.html. R sistance microbienne aux m ...
Broad goals are to define the mechanisms of blood clotting by Staphylococcus aureus staphylocoagulase (SC) and von Willebrand factor binding-protein (vWbp), and...
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
Staphylococcus, referred as Staph, is a bacterial organism that can affect anyone. Staph is a common germ that 25% to 30% of people carry on their skin, and staph can even be present in your nose. You can easily be infected with staph through an injury or break in the skin, just by casual contact. Infections can result in minor to severe symptoms, even leading to fatality. If someone in your household is infected with staph, it is very important that you kill the germs to prevent the disease from spreading.. ...
L.L. Bean must make stocking decisions on thousands of items sold through its catalogs. In many cases, orders must be placed with vendors twelve or more weeks before a catalog lands on a customers doorstep, and commitments cannot be changed thereafter. As a result, L.L. Bean suffers annual losses of over $20 million due to stockouts or liquidations of excess inventory. Provides a context in which buying decisions that balance costs of overstocking and understocking when demand is uncertain are made and implemented on a routine basis ...
Staph Infection has been identified as the culprit for multiple deaths around the United States thus far as reported by NewsDay. Super Staph Infection (MRSA) is spreading globally at pandemic rates and it is only a matter of time before Staph Infection makes an appearance in your institution if it hasnt already made its way there. The time is now to ensure your guests, patients, students, residents and the public an environment free of harmful microorganisms. PuraCleenRx™ products have been formulated specifically to protect your institution from the harmful bacteria, mold, mildew, viruses, fungi and blood pathogens trying to enter your environment.. To start ensuring that your facility has the best protection possible against Staph Infection click here NOW![…]. PuraCleenRx products include a broad spectrum of anti-microbial solutions. Our products take on more than any anti-bacterial product on the market eliminating bacteria, viruses, blood pathogens and fungi including new strands of ...
K.H. Schleifer <- W.E. Kloos, MCS 11. Human skin (614). Taxonomy/description (614). Murein: A11.3; teichoic acid: glycerol (614). (Medium 53, 37°C ...
Staph skin infections are considered to be the most common staph infections and the symptoms of staph infection do vary according to the infection type bacteria
Staph or staphylococcus bacteria often live on the skin or nose of healthy people, causing no harm. However, staph can cause infections that can be...
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October 17, 2007. ABOUT 18,700 people die in this country each year from drug-resistant staph infections, according to a federal study released Tuesday - more deaths than the United States sees from AIDS annually.. Full Report. ...
Staph`y*lora*phy, Staph`y*lorrha*phy (?), n. [Gr. the uvula when swollen + to sew: cf. F. staphylorraphie.] The operation of...
TY - JOUR. T1 - The superoxide dismutase gene sodM is unique to Staphylococcus aureus. T2 - Absence of sodM in coagulase-negative staphylococci. AU - Wright Valderas, Michelle. AU - Gatson, Joshua W.. AU - Wreyford, Natalie. AU - Hart, Mark E.. PY - 2002. Y1 - 2002. N2 - Superoxide dismutase (SOD) profiles of clinical isolates of Staphylococcus aureus and coagulase-negative staphylococci (CONS) were determined by using whole-cell lysates and activity gels. All S. aureus clinical isolates exhibited three closely migrating bands of activity as previously determined for laboratory strains of S. aureus: SodM, SodA, and a hybrid composed of SodM and SodA (M. W. Valderas and M. E. Hart, J. Bacteriol. 183:3399-3407, 2001). In contrast, the CoNS produced only one SOD activity, which migrated similarly to SodA of S. aureus. Southern analysis of eight CoNS species identified only a single sod gene in each case. A full-length sod gene was cloned from Staphylococcus epidermidis and determined to be more ...
This study was conducted to determine the frequency of Staphylococcus lugdunensis in different clinical samples. Out of 690 clinical samples, a total of 178 coagulase negative staphylococci (CoNS) isolates were recovered. CoNS were identified as 10 different species; 22 isolates belonged to Staphylococcus lugdunensis. Two specific genes for S. lugdunensis were used (tanA gene and fbl gene) to confirm identification. Both of these specific genes were detected in 15 (68.1%) of 22 isolates that were identified phenotypically. The results of oxacillin MIC showed that 7 of the 15 (46.6%) S. lugdunensis isolates were oxacillin resistant. The antibiotic susceptibility testing against 16 antibiotics showed that resistance rates were variable towards these antibiotics. Eight of fifteen S. lugdunensis isolates (53.3%) were β-lactamase producer. Results of molecular detection of mecA gene found that mecA gene was detected in 6 (40%) of 15 S. lugdunensis. All of these 6 isolates (S1, S2, S3, S4, S5, and ...
TY - JOUR. T1 - From clinical microbiology to infection pathogenesis. T2 - How daring to be different works for Staphylococcus lugdunensis. AU - Frank, Kristi L.. AU - Del Pozo, José Luis. AU - Patel, Robin. PY - 2008/1. Y1 - 2008/1. N2 - Staphylococcus lugdunensis has gained recognition as an atypically virulent pathogen with a unique microbiological and clinical profile. S. lugdunensis is coagulase negative due to the lack of production of secreted coagulase, but a membrane-bound form of the enzyme present in some isolates can result in misidentification of the organism as Staphylococcus aureus in the clinical microbiology laboratory. S. lugdunensis is a skin commensal and an infrequent pathogen compared to S. aureus and S. epidermidis, but clinically, infections caused by this organism resemble those caused by S. aureus rather than those caused by other coagulase-negative staphylococci. S. lugdunensis can cause acute and highly destructive cases of native valve endocarditis that often ...
The objective of the present study was to determine the antimicrobial resistance profile of planktonic and biofilm cells of Staphylococcus aureus and coagulase-negative staphylococci (CoNS). Two hundred Staphylococcus spp. strains were studied, including 50 S. aureus and 150 CoNS strains (50 S. epidermidis, 20 S. haemolyticus, 20 S. warneri, 20 S. hominis, 20 S. lugdunensis, and 20 S. saprophyticus). Biofilm formation was investigated by adherence to polystyrene plates. Positive strains were submitted to the broth microdilution method to determine the minimum inhibitory concentration (MIC) for planktonic and biofilm cells and the minimal bactericidal concentration for biofilm cells (MBCB). Forty-nine Staphylococcus spp. strains (14 S. aureus, 13 S. epidermidis, 13 S. saprophyticus, 3 S. haemolyticus, 1 S. hominis, 3 S. warneri, and 2 S. lugdunensis) were biofilm producers. These isolates were evaluated regarding their resistance profile. Determination of planktonic cell MIC identified three (21.4%) S.
Discussion. Oxacillin resistance represents a major problem in the treatment of staphylococcal infections, especially because of the heterogenous expression of this resistance (Tveten et al., 2004). In the present study, oxacillin resistance was detected by PCR in 85 (82.5%) of the CoNS isolates studied. Similar results have been reported by Perez and dAzevedo (2008), who reported a rate of 78.4% of oxacillin-resistant CoNS strains using amplification of the mecA gene as the gold standard.. In addition to detection of the mecA gene, the reference methods recommended by the CLSI for the detection of oxacillin resistance in CoNS include the determination of MICs by agar and broth dilution methods, disk diffusion method, screening on Mueller-Hinton agar supplemented with 4 µg/ml oxacillin and 4% NaCl and, more recently, the cefoxitin disk diffusion test (NCCLS 1999, CLSI 2009). The present results show a sensitivity of 95.3% of the oxacillin disk diffusion method for the detection of MRCoNS. A ...
Staphylococcus saprophyticus is an important cause of urinary tract infection (UTI), particularly among young women, and is second only to uropathogenic Escherichia coli as the most frequent cause of UTI. The molecular mechanisms of urinary tract colonization by S. saprophyticus remain poorly understood. We have identified a novel 6.84 kb plasmid-located adhesin-encoding gene in S. saprophyticus strain MS1146 which we have termed uro-adherence factor B (uafB). UafB is a glycosylated serine-rich repeat protein that is expressed on the surface of S. saprophyticus MS1146. UafB also functions as a major cell surface hydrophobicity factor. To characterize the role of UafB we generated an isogenic uafB mutant in S. saprophyticus MS1146 by interruption with a group II intron. The uafB mutant had a significantly reduced ability to bind to fibronectin and fibrinogen. Furthermore, we show that a recombinant protein containing the putative binding domain of UafB binds specifically to fibronectin and ...
Staphylococcus aureus and coagulase negative staphylococci (CNS) were isolated from ovine and caprine mastitis milk samples originating from more than 40 Swiss farms. CNS dominated as causal microorganisms of mastitis in small ruminants. By restriction fragment length polymorphism (RFLP) analysis of the groEL gene and sequencing of 16S rDNA, various CNS species were identified, albeit certain of them predominated. For susceptibility testing of mastitis pathogens to selected antibiotics, minimal inhibitory concentrations were determined. Of the 67 S. aureus and 208 CNS strains, 31.3 % and 8.2 % were resistant to penicillin, 29.9 % and 1.0 % to ampicillin, 1.5 % and 10.6 % to erythromycin, and 3.0 % and 7.7 % to tetracycline, respectively. Moreover, 10 CNS strains (4.8 %) were resistant to oxacillin and one CNS strain to sulfamethoxazole/trimethoprim. The results obtained describe for the first time the resistance situation of mastitis pathogens from sheep and goats in Switzerland. However, ...
We have reviewed 107 cases of staphylococcal bacteraemia in order to assess the current clinical spectrum of serious staphylococcal sepsis in Zimbabwe, where staphylococcal bacteraemia is common. Infection was hospital-acquired in 35 cases and community-acquired in 72 cases. The mortality rate was 28%. Most patients were young, with predisposing conditions such as prematurity, protein-caloric maln...
... : Ch.19 L.2 (More about staphylococcus, Folliculitis, Enzymes (Coagulase, Hyaluronidase, Staphylokinase, Lipases, β-Lactamase, Virulent strains of S. aureus make several enzymes that help them to survive:), Toxins (Cytolytic toxins, Leukocidin, Epidermal dell differentiation inhibitor , Exfoliative toxin or toxic shock syndrome toxin), Staphylococcus aureus, Staphylococcus epidermidis)
Abstract : Staphylococcus saprophyticus (S. saprophyticus) is a frequent cause of urinary tract infection in the young women.The current study was designed to analyze the effect of biofield energy treatment on S. saprophyticus for evaluationof its antibiogram profile, biochemical reactions pattern and biotyping characteristics. Two sets of ATCC sampleswere taken in this experiment and denoted as A and B. Sample A was revived and divided into two parts Group (Gr.I)(control) and Gr.II (revived); likewise, sample B was labeled as Gr.III (lyophilized). Gr. II and III were given with Mr.Trivedis biofield energy treatment. The control and treated groups of S. saprophyticus cells were tested with respect toantimicrobial susceptibility, biochemical reactions pattern and biotype number using MicroScan Walk-Away® system.The 50% out of twenty-eight tested antimicrobials showed significant alteration in susceptibility and 36.67% out of thirtyantimicrobials showed an alteration in minimum inhibitory ...
Results Out of 503 children with ABM, there were 199 cases in 2012 and 304 in 2013-2015. Frequency of meningitis decreased by 50%.Most children in both groups were under one year. Relatively less children were fully vaccinated in new group, 30.3% vs 45%.Increased complications seen in the newer group 20% vs 17%.CSF culture positivity decreased from (12%) to (6.6%). The common pathogens identified on CSF culture were Coagulase negative staphylococci (CoNS) 11 (5.5%), Streptococcus pneumoniae 5 (2.5%), H. influenzae 2 (1%) in 2012, whereas in the new group Klebsiella 7 (2.3%) was the most common followed by Streptococcus pneumoniae 4 (1.3%), and (CoNS) 3 (1%). Refusal to take feed (p=0.002), impaired sensorium (p=,0.001), severe malnutrition (p=0.001), prolonged duration of symptoms (p=,0.001)and incomplete vaccination status (0.005)were associated with mortality. Mortality rate decreased from 20 (10%) in 2012 to 17 (5.6%) in 2013-2015 but more children developed neurological squealier 2.7% versus ...
The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc), enterotoxin A (entA), and the gene segments encoding the immunoglobulin G binding region and the X region of protein A gene spa. All of the isolates yielded an amplicon with a size of approximately 1,042 bp of the clfA gene. The amplification of the polymorphic spa gene segment encoding the immunoglobulin G binding region was observed in 34 isolates and X-region binding was detected in 26 isolates. Amplification of the coa gene yielded three different products in 20, 10, and 7 isolates. The amplification of the ...
Staphylococcus saprophyticus ATCC ® 43867™ Designation: LRA 27.02.80 TypeStrain=False Application: Quality control strain
We describe here a strategy that can distinguish between Staphylococcus species truly present in a clinical sample from contaminating Staphylococcus species introduced during the testing process. Contaminating Staphylococcus species are present at low levels in PCR reagents and colonize lab personnel. To eliminate detection of contaminants, we describe an approach that utilizes addition of sufficient quantities of either non-target Staphylococcal cells (Staphylococcus succinus or Staphylococcus muscae) or synthetic oligonucleotide templates to helicase dependent isothermal amplification reactions to consume Staphylococcus-specific tuf and mecA gene primers such that contaminating Staphylococcus amplification is suppressed to below assay limits of detection. The suppressor template DNA is designed with perfect homology to the primers used in the assay but an internal sequence that is unrelated to the Staphylococcal species targeted for detection. Input amount of the suppressor is determined by a
Bacterial infections are common in hematological malignancy. Coagulase-negative staphylococci (CoNS) are among the most prevalent causes of bacteremia in patients with hematological malignancies.. In this thesis, different aspects of CoNS in hematological malignancy have been studied in four papers:. In paper 1, CoNS blood culture isolates from patients with hematological malignancies treated at the University Hospital of Örebro from 1980 to 2009 were revaluated for the presence of reduced sensitivity to glycopeptides. A high incidence of heterogeneous-intermediate glycopeptide resistance was observed and there was a trend towards increasing incidence of this phenotype over time.. In paper 2, the colonization pattern of CoNS among patients undergoing intensive chemotherapy for hematological malignancy was investigated. A successive homogenization and an accumulation of CoNS phenotypes mutually present in a majority of included patients were demonstrated.. In paper 3, a PCR method to determine ...
Bacterial infections are common in hematological malignancy. Coagulase-negative staphylococci (CoNS) are among the most prevalent causes of bacteremia in patients with hematological malignancies.. In this thesis, different aspects of CoNS in hematological malignancy have been studied in four papers:. In paper 1, CoNS blood culture isolates from patients with hematological malignancies treated at the University Hospital of Örebro from 1980 to 2009 were revaluated for the presence of reduced sensitivity to glycopeptides. A high incidence of heterogeneous-intermediate glycopeptide resistance was observed and there was a trend towards increasing incidence of this phenotype over time.. In paper 2, the colonization pattern of CoNS among patients undergoing intensive chemotherapy for hematological malignancy was investigated. A successive homogenization and an accumulation of CoNS phenotypes mutually present in a majority of included patients were demonstrated.. In paper 3, a PCR method to determine ...
Indicated for the treatment of otitis externa in dogs associated with susceptible strains of yeast (Malassezia pachydermatis) and bacteria (coagulase positive staphylococci, Pseudomonas aeruginosa and Enterococcus faecalis ...
impact of vacuum packing of smoked fish (trout) and modified atmosphere on maintainability and protection of quality parameters. The presence of other pathogens (Salmonella spp., coagulase positive Staphylococci, sulphite-reducing Clostridia) in cold smoked packed trout was also investigated. The research was conducted regarding the change of the total number of bacteria, and the change of the number of lactic acid bacteria, as well as the number of Listeria monocytogenes in samples of cold smoked trout vacuum packed, under modified atmosphere and stored at the temperatures of 3°C and 8°C, respectively. During 35 days of storage at the temperatures of 3°C and 8°C, respectively, the total number of bacteria was increasing until day 14 and day 28 subject to a group, only to be at the level of number of bacteria on day 0 or less. The average total number of bacteria in samples of cold vacuumed smoked trout, or at modified atmosphere was less during the research in samples that were stored at ...
S. epidermidis, a coagulase-negative staphylococcus species, is a commensal of the skin, but can cause severe infections in ... S. saprophyticus, another coagulase-negative species that is part of the normal vaginal flora, is predominantly implicated in ... The main coagulase-positive staphylococcus is Staphylococcus aureus, although not all strains of Staphylococcus aureus are ... Becker, Karsten; Heilmann, Christine; Peters, Georg (2014). "Coagulase-Negative Staphylococci". Clinical Microbiology Reviews. ...
S. caprae is coagulase-negative. It was originally isolated from goats (caprae means "of a goat"), but members of this species ... It is a coagulase-negative, DNase-positive member of the genus Staphylococcus. Usually it is associated with goats. Since 1991 ... "Identification of coagulase-negative staphylococci other than Staphylococcus epidermidis by automated ribotyping". Clin ...
The coagulase test determines whether an organism can produce the enzyme coagulase, which causes the fibrin to clot. ... A clot indicates the presence of coagulase, while no clot indicates the lack of coagulase. DNase agar is used to test whether a ... "Welcome to Microbugz - Coagulase Test". www.austincc.edu. Retrieved 2017-04-03. "Welcome to Microbugz - DNase Test". www. ... Inoculating a plasma test tube with the microbe indicates whether coagulase is produced. ...
nov., a coagulase-variable species from bovine subclinical and mild clinical mastitis". International Journal of Systematic and ... Staphylococcus agnetis is a Gram positive, coagulase-variable member of the bacterial genus Staphylococcus. Strains of this ... "Coagulase-positive staphylococci: my pet's two faces". Research in Microbiology. 164 (5): 371-374. doi:10.1016/j.resmic.2013.02 ...
nov., a coagulase-positive species from animals". International Journal of Systematic and Evolutionary Microbiology. 55 (4): ...
It is a catalase-positive, coagulase-negative, facultative anaerobe that can grow by aerobic respiration or by fermentation. ... ISBN 1-55581-171-X. Queck SY & Otto M (2008). "Staphylococcus epidermidis and other Coagulase-Negative Staphylococci". ... which is coagulase-negative, as well, but novobiocin-resistant. Similar to those of S. aureus, the cell walls of S. epidermidis ... catalase and slide coagulase testing. On the Baird-Parker agar with egg yolk supplement, colonies appear small and black. ...
Many of the isolated strains show coagulase activity. Clinical tests for detection of methicillin-resistant S. aureus may ...
nov., a Coagulase-Positive Species Isolated from Dolphins". International Journal of Systematic Bacteriology. 38 (4): 436-439. ... Staphylococcus delphini is a Gram-positive, coagulase-positive member of the bacterial genus Staphylococcus consisting of ...
The opposite of coagulase, kinases can dissolve clots. S. aureus can also produce staphylokinase, allowing them to dissolve the ... By binding to prothrombin, coagulase facilitates clotting in a cell by ultimately converting fibrinogen to fibrin. Bacteria ...
S. aureus is coagulase-positive, meaning it produces coagulase. However, while the majority of S. aureus strains are coagulase- ... Seven species are currently recognised as being coagulase-positive: S. aureus, S. delphini, S. hyicus, S. intermedius, S. ... A new coagulase negative species - Staphylococcus edaphicus - has been isolated from Antarctica.[10] This species is probably a ... All species of Staphylococcus aureus were once thought to be coagulase-positive, but this has since been disproven.[14][15][16] ...
S. aureus is coagulase-positive, meaning it produces coagulase. However, while the majority of S. aureus strains are coagulase- ... S. epidermidis, a coagulase-negative species, is a commensal of the skin, but can cause severe infections in immunosuppressed ... S. saprophyticus, another coagulase-negative species that is part of the normal vaginal flora, is predominantly implicated in ... All species were once thought to be coagulase-positive, but this has since been disproven. Growth can also occur in a 6.5% NaCl ...
nov.: Three New Coagulase-Negative, Novobiocin-Resistant Species from Animals". Systematic and Applied Microbiology. 5 (4): 501 ... Staphylococcus arlettae is a gram-positive, coagulase-negative member of the bacterial genus Staphylococcus consisting of ...
Strains of this species were originally isolated from otters and are coagulase-positive. Foster, G.; Ross, H. M.; Hutson, R. A ... nov., a New Coagulase-Positive Species Isolated from Otters". International Journal of Systematic Bacteriology. 47 (3): 724-726 ...
S. aureus produces various enzymes such as coagulase (bound and free coagulases) which clots plasma and coats the bacterial ... Previously, S. aureus was differentiated from other staphylococci by the coagulase test. However, not all S. aureus strains are ... Hanselman BA, Kruth SA, Rousseau J, Weese JS (2009). "Coagulase positive staphylococcal colonization of humans and their ... coagulase (fibrin clot formation, positive for S. aureus), DNAse (zone of clearance on DNase agar), lipase (a yellow color and ...
... is a coagulase-negative species (CoNS) of Staphylococcus. It is part of the normal flora of the skin of ... Kloos WE PMC 358308, Update on clinical significance of coagulase-negative staphylococci. Clin Microbiol Rev. 1994 Jan; 7(1): ... 117-140 Coagulase Negative Staphylococcus CNS Type strain of Staphylococcus capitis at BacDive - the Bacterial Diversity ...
Numerous coagulase-negative staphylococci appear commonly on the skin of human. Of these species, S. epidermidis and S. hominis ... Like many other coagulase-negative staphylococci, S. hominis may occasionally cause infection in patients whose immune systems ... More undocumented instances of SHN infections may not have been reported because not all coagulase-negative staphlococcal ... Staphylococcus hominis is a coagulase-negative member of the bacterial genus Staphylococcus, consisting of Gram-positive, ...
Like other coagulase-negative staphylococci, S. warneri rarely causes disease, but may occasionally cause infection in patients ... 1986) Identification of coagulase-negative Staphylococci isolated from urinary tract infections. Am. J. Clin. Pathol. 85(1):92- ... It is catalase-positive, oxidase-negative, and coagulase-negative, and is a common commensal organism found as part of the skin ...
... is a member of the coagulase-negative staphylococci (CoNS). It is part of the skin flora of humans ... 2001). "Rapid and Accurate Species-Level Identification of Coagulase-Negative Staphylococci by Using the sodA Gene as a Target ... 2002). "The ica Operon and Biofilm Production in Coagulase-Negative Staphylococci Associated with Carriage and Disease in a ... 2007). "Persistent strains of coagulase-negative staphylococci in a neonatal intensive care unit: virulence factors and ...
In the laboratory, it can give a positive slide-coagulase test but a negative tube-coagulase test. It is fairly easy to ... They also can have a characteristic sweet, hay-like odor.[citation needed] S. lugdunensis may produce a bound coagulase (that ... Staphylococcus lugdunensis is a coagulase-negative member of the genus Staphylococcus, consisting of Gram-positive bacteria ... Babu, E.; Oropello, J. (2011). "Staphylococcus lugdunensis: The coagulase-negative staphylococcus you don't want to ignore". ...
Pereira VC, Cunha Mde L (November 2013). "Coagulase-negative staphylococci strains resistant to oxacillin isolated from ...
It is coagulase-negative and is probably a commensal organism on the skin of humans. Colonies are 1-2 mm in diameter, sometimes ... nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens. Int. J. Syst. Evol. Microbiol. ... Like other coagulase-negative staphylococci, S. pettenkoferi only rarely causes disease, but may occasionally cause infection ...
Like most staphylococcal species, it is coagulase-negative and exists as a commensal on the skin of humans and animals and in ... Staphylococcus xylosus may be used as CNC (coagulase-negative cocci) in salami fermentation. It appears to be far more common ... Aquilanti, L., Garofalo, C., Osimani, A. and Clementi, F.: Ecology of lactic acid bacteria and coagulase negative cocci in ...
It is identitified as a species of coagulase-negative staphylococci (CoNS) using the coagulase test. Lastly, S. saprophyticus ... Prior to this, the presence of coagulase-negative staphylococci (CoNS) in urine specimens was dismissed as contamination.[ ... 94-9. Widerström, M; Wiström, J; Sjöstedt, A; Monsen, T (January 2012). "Coagulase-negative Staphylococci: Update on the ... Staphylococcus saprophyticus is a Gram-positive coccus belonging to the coagulase-negative Staphylococcus genus. S. ...
Tefibazumab Coagulase Hair PS, Ward MD, Semmes OJ, Foster TJ, Cunnion KM (July 2008). "Staphylococcus aureus clumping factor A ...
Resch, M; Nagel, V; Hertel, C (Sep 30, 2008). "Antibiotic resistance of coagulase-negative staphylococci associated with food ... Staphylococcus condimenti is a Gram-positive, coagulase-negative member of the bacterial genus Staphylococcus consisting of ...
Coagulase Fluoroquinolones Methicillin-resistance Resistência microbiana a drogas Infecções oculares bacterianas Staphylococcus ... Suscetibilidade dos Staphylococcus coagulase negativo meticilina-resistentes e suscetíveis isolados em infecções oculares. ... METHODS: The minimum inhibitory concentrations (MICs) of 21 strains of methicillin-resistant coagulase-negative staphylococci ( ... CONCLUSION: Our results indicate that methicillin-sensitive coagulase-negative staphylococci are more susceptible to quinolones ...
Staphylococcus haemolyticus is a member of the coagulase-negative staphylococci (CoNS).[2] It is part of the skin flora of ... 2007). "Persistent strains of coagulase-negative staphylococci in a neonatal intensive care unit: virulence factors and ... 2011). "Antibiotic resistance and molecular characterization of clinical isolates of methicillin-resistant coagulase-negative ... it tests negative for coagulase, DNase, ornithine decarboxylase, phosphatase, urease, and oxidase.[2] ...
von Eiff, C., Peters, G., and Heilmann, C. (2002). Pathogenesis of infections due to coagulase-negative staphylococci. Lancet ... 2005). Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic ...
In the laboratory, it can give a positive slide-coagulase test but a negative tube-coagulase test. It is fairly easy to ... They also can have a characteristic sweet, hay-like odor.[citation needed] S. lugdunensis may produce a bound coagulase (that ... Staphylococcus lugdunensis is a coagulase-negative member of the genus Staphylococcus, consisting of Gram-positive bacteria ... Babu, E.; Oropello, J. (2011). "Staphylococcus lugdunensis: The coagulase-negative staphylococcus you dont want to ignore". ...
S.aureus produces two forms of coagulase (i.e., bound coagulase and free coagulase). Bound coagulase, otherwise known as " ... ISBN 0-8151-2535-6. "coagulase test" at Dorlands Medical Dictionary Tube coagulase test - rabbit plasma video Coagulase at the ... and free coagulase can be detected using a tube coagulase test. A slide coagulase test is run with a negative control to rule ... Bound coagulase is part of the larger family of MSCRAMM. The coagulase test has traditionally been used to differentiate ...
Coagulase-negative Staphylococci (CNS) are known to cause distinct types of infections in humans like endocarditis and urinary ... Coagulase-negative Staphylococci (CNS) are known to cause distinct types of infections in humans like endocarditis and urinary ... Anderson-Berry A, Brinton B, Lyden E, Faix RG (2011) Risk factors associated with development of persistent coagulase negative ... Venkatesh MP, Placencia F, Weisman LE (2006) Coagulase negative staphylococcal infections in the neonate and child: an update. ...
K. L. Rogers, P. D. Fey, and M. E. Rupp, "Coagulase-negative Staphylococcal infections," Infectious Disease Clinics of North ... Neonatal Sepsis due to Coagulase-Negative Staphylococci. Elizabeth A. Marchant,1,2 Guilaine K. Boyce,1,2 Manish Sadarangani,3,4 ... J. Huebner and D. A. Goldmann, "Coagulase-negative staphylococci: role as pathogens," Annual Review of Medicine, vol. 50, pp. ... S. L. Hall, S. W. Riddell, W. G. Barnes, L. Meng, and R. T. Hall, "Evaluation of coagulase-negative staphylococcal isolates ...
Coagulase-negative staphylococcus in chronic prostatitis.. Nickel JC1, Costerton JW.. Author information. 1. Department of ... Coagulase-negative staphylococci were cultured in the biopsied prostatic tissue, and gram-positive staphylococci were ... Coagulase-negative staphylococci may be implicated in the pathogenesis of chronic bacterial prostatitis. ... Three male patients with a clinical history of prostatitis with coagulase-negative staphylococci localized to the expressed ...
Coagulase Test - Tube Test. ... List of coagulase-positive staphylococci Staphylococcus aureus subsp ... List of coagulase- ... Other articles related to coagulase:. Staphylococcal Infection - Coagulase-negative. ... epidermidis, a coagulase-negative ... Coagulase. Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin ... Staphylococcal Infection - Coagulase-positive. ... aureus#Role in disease The main coagulase-positive staphylococcus is ...
... Description. Also referred to as Staphylococcus aureus test. Foods that are frequently ...
A total of 414 coagulase-positive staphylococcal strains obtained at the mastitis laboratory, National Veterinary Institute, ... Identification of coagulase-positive staphylococci isolated from bovine milk Acta Vet Scand. 1999;40(4):315-21. ... These 3 tests are simple and quick to perform and enable accurate for easy differentiation of the 3 coagulase-positive ... A total of 414 coagulase-positive staphylococcal strains obtained at the mastitis laboratory, National Veterinary Institute, ...
Coagulase-negative Staphylococci (CoNS) are a major cause of postoperative infections. These infections are often associated ... Study of developed resistance due to antibiotic treatment of coagulase-negative Staphylococci.. Tegnell A1, Grabowska K, ...
This novel bi-functional protein consists of truncated coagulase (tCoa) and an NGR (GNGRAHA) motif that recognizes CD13 and ... Coagulase activity assays. Coagulase activity was assessed as previously described [14, 44]. Briefly, human prothrombin (1% ... Furthermore, it was reported that truncated coagulase (~325 aa) is sufficient for coagulase activity, whereas the N-terminal ... since coagulase binds to free thrombin, it acts as more of a scavenger of this protein [20]. Coagulase also has relatively poor ...
Staphylococcus simulans (coagulase negative), MicroKwik Culture®, Pathogen, Vial. Item # 155555A *bvseo_sdk, java_sdk, bvseo- ... The coagulase negative culture is Gram+, nonmotile cocci in irregular clusters; does not clot blood plasma. It is cultured on ...
Coagulase-negative staphylococci (CNS) have become the most common bovine mastitis isolate in many countries and could ... Coagulase-negative Staphylococci-Emerging Mastitis Pathogens Vet Microbiol. 2009 Feb 16;134(1-2):3-8. doi: 10.1016/j.vetmic. ... Coagulase-negative staphylococci (CNS) have become the most common bovine mastitis isolate in many countries and could ...
References for Abcams Anti-Staphylococcus aureus coagulase Type I antibody (ab79636). Please let us know if you have used this ...
Production of siderophore by coagulase-negative staphylococci and its relation to virulence.. Lindsay JA1, Riley TV, Mee BJ. ... To determine if siderophore production by coagulase-negative staphylococci (CNS) was related to virulence, 40 clinical isolates ...
Are coagulase-negative Staphylococcus (CoNS) species important? Although the clinical significance of CoNS isolated in culture ... Laboratory Detection of Coagulase-negative Staphylococcus species with Decreased Susceptibility to the Glycopeptides Vancomycin ... Emergence of vancomycin resistance in coagulase-negative staphylococci. New England Journal of Medicine 316:927-931. ...
Expression of Coagulase and Mechanism of Fibrinolysis. E. D. Beesley, R. R. Brubaker, W. A. Janssen, M. J. Surgalla ... Expression of Coagulase and Mechanism of Fibrinolysis Message Subject (Your Name) has forwarded a page to you from Journal of ... Coagulase and the fibrinolytic factor were not differentiated by these criteria. Spontaneous suppressor mutations causing ... Attempts to demonstrate a common activator of pesticin I, coagulase, or the fibrinolytic factor in extracts of pesticinogenic ...
Antibiotic Resistance in Staphylococcus aureus and Coagulase Negative Staphylococci Isolated from Goats with Subclinical ... aureus and 75 Coagulase Negative Staphylococci (CNS) strains isolates from half-udder milk samples collected from goats with ...
"Speciation of Coagulase-Negative Staphylococcus Causing Bacterial Keratitis," which appeared in a 2005 issue of the "Indian ...
J. F. Veras, L. S. Carmo, L. C. Tong et al., "A study of the enterotoxigenicity of coagulase-negative and coagulase-positive ... "Prevalence of enterotoxin-encoding genes and antimicrobial resistance in coagulase-negative and coagulase-positive ... F. Irlinger, "Safety assessment of dairy microorganisms: coagulase-negative staphylococci," International Journal of Food ... E. Coton, M.-H. Desmonts, S. Leroy et al., "Biodiversity of Coagulase-Negative Staphylococci in French cheeses, dry fermented ...
Raquel Soares Casaes Nunes, Eduardo Mere Del Aguila, and Vânia Margaret Flosi Paschoalin, "Safety Evaluation of the Coagulase- ... Safety Evaluation of the Coagulase-Negative Staphylococci Microbiota of Salami: Superantigenic Toxin Production and ...
"Coagulase-negative Staphylococcal infections". Infect Dis Clin N Am. vol. 23. 2009. pp. 73-98. ... There are more than 45 recognized species of coagulase-negative staphylococci (CoNS). CoNS are gram-positive cocci that divide ... in irregular "grape-like" clusters and are differentiated from S. aureus by their inability to produce coagulase and coagulate ...
Coagulase-negative staphylococci can colonize the surface of catheters and produce an extracellular polysaccharide that leads ... Notify me of updates to Staphylococcus aureus and Coagulase-Negative Staphylococci: Virulence, Antimicrobial Resistance and ... Chapter 2. Clinical Significance of Coagulase-Negative Staphylococci Chapter 3. Catheter-Related Infections Chapter 4. ... Staphylococcus aureus and Coagulase-Negative Staphylococci: Virulence, Antimicrobial Resistance and Molecular Epidemiology. $ ...
  • This study evaluated polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as a molecular typing technique for MRSA strains on the basis of protein A ( spa ) and coagulase ( coa ) gene polymorphisms to verify their ability in assessing the relatedness of isolates. (hindawi.com)
  • Molecular epidemiology of coagulase-negative staphylococcal bacteraemia in a newborn intensive care unit. (semanticscholar.org)
  • As amostras foram submetidas a contagem de Staphylococcus coagulase positiva, identificação molecular da espécie e de genes de enterotoxinas clássicas, bem como sua produção in vitro, alem de teste de suscetibilidade a antimicrobianos. (ufpr.br)
  • The prerequisites for food poisoning via coagulase-positive staphylococci are that the pathogens have sufficiently multiplied in the food product and that heat-stable enterotoxins have been secreted by the pathogens. (bund.de)
  • Coagulase" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (umassmed.edu)
  • The coagulase-producing and clumping factor-producing parent strain Staphylococcus aureus Newman and a series of mutants defective in either coagulase, clumping factor, or both were tested for their ability (i) to attach in vitro to either rat fibrinogen or platelet-fibrin clots and (ii) to produce endocarditis in rats with catheter-induced aortic vegetations. (asm.org)