A highly conserved heterodimeric glycoprotein that is differentially expressed during many severe physiological disturbance states such as CANCER; APOPTOSIS; and various NEUROLOGICAL DISORDERS. Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Serum proteins that negatively regulate the cascade process of COMPLEMENT ACTIVATION. Uncontrolled complement activation and resulting cell lysis is potentially dangerous for the host. The complement system is tightly regulated by inactivators that accelerate the decay of intermediates and certain cell surface receptors.
The network of channels formed at the termination of the straight SEMINIFEROUS TUBULES in the mediastinum testis. Rete testis channels drain into the efferent ductules that pass into the caput EPIDIDYMIS.

Recovery following relief of unilateral ureteral obstruction in the neonatal rat. (1/535)

BACKGROUND: Obstructive nephropathy is a primary cause of renal insufficiency in infants and children. This study was designed to distinguish the reversible and irreversible cellular consequences of temporary unilateral ureteral obstruction (UUO) on the developing kidney. METHODS: Rats were subjected to UUO or sham operation in the first 48 hours of life, and the obstruction was removed five days later (or was left in place). Kidneys were removed for study 14 or 28 days later. In additional groups, kidneys were removed at the end of five days of obstruction. Immunoreactive distribution of renin was determined in arterioles, and the distribution of epidermal growth factor, transforming growth factor-beta1, clusterin, vimentin, and alpha-smooth muscle actin was determined in tubules and/or interstitium. The number of glomeruli, glomerular maturation, tubular atrophy, and interstitial collagen deposition was determined by morphometry. Renal cellular proliferation and apoptosis were measured by proliferating cell nuclear antigen and the TdT uridine-nick-end-label technique, respectively. The glomerular filtration rate was measured by inulin clearance. RESULTS: Renal microvascular renin maintained a fetal distribution with persistent UUO; this was partially reversed by the relief of obstruction. Although glomerular maturation was also delayed and glomerular volume was reduced by UUO, the relief of obstruction prevented the reduction in glomerular volume. Although relief of obstruction did not reverse a 40% reduction in the number of nephrons, the glomerular filtration rate of the postobstructed kidney was normal. The relief of obstruction did not improve tubular cell proliferation and only partially reduced apoptosis induced by UUO. This was associated with a persistent reduction in the tubular epidermal growth factor. In addition, the relief of obstruction reduced but did not normalize tubular expression of transforming growth factor-beta1, clusterin, and vimentin, all of which are evidence of persistent tubular injury. The relief of obstruction significantly reduced interstitial fibrosis and expression of alpha-smooth muscle actin by interstitial fibroblasts, but not to normal levels. CONCLUSIONS: The relief of obstruction in the neonatal rat attenuates, but does not reverse, renal vascular, glomerular, tubular, and interstitial injury resulting from five days of UUO. Hyperfiltration by remaining nephrons and residual tubulointerstitial injury in the postobstructed kidney are likely to lead to deterioration of renal function later in life.  (+info)

Clusterin has chaperone-like activity similar to that of small heat shock proteins. (2/535)

Clusterin is a highly conserved protein which is expressed at increased levels by many cell types in response to a broad variety of stress conditions. A genuine physiological function for clusterin has not yet been established. The results presented here demonstrate for the first time that clusterin has chaperone-like activity. At physiological concentrations, clusterin potently protected glutathione S-transferase and catalase from heat-induced precipitation and alpha-lactalbumin and bovine serum albumin from precipitation induced by reduction with dithiothreitol. Enzyme-linked immunosorbent assay data showed that clusterin bound preferentially to heat-stressed glutathione S-transferase and to dithiothreitol-treated bovine serum albumin and alpha-lactalbumin. Size exclusion chromatography and SDS-polyacrylamide gel electrophoresis analyses showed that clusterin formed high molecular weight complexes (HMW) with all four proteins tested. Small heat shock proteins (sHSP) also act in this way to prevent protein precipitation and protect cells from heat and other stresses. The stoichiometric subunit molar ratios of clusterin:stressed protein during formation of HMW complexes (which for the four proteins tested ranged from 1.0:1.3 to 1.0:11) is less than the reported ratios for sHSP-mediated formation of HMW complexes (1.0:1.0 or greater), indicating that clusterin is a very efficient chaperone. Our results suggest that clusterin may play a sHSP-like role in cytoprotection.  (+info)

Clusterin gene expression mediates resistance to apoptotic cell death induced by heat shock and oxidative stress. (3/535)

Clusterin is a widely expressed, well conserved, secreted glycoprotein, which is highly induced in tissues regressing as a consequence of apoptotic cell death in vivo. It has recently been shown that clusterin expression is only confined to surviving cells following the induction of apoptosis in vitro, suggesting that it is involved in cell survival rather than death. In the hypothesis that clusterin may be implicated in cellular responses to stress, clusterin gene expression was analyzed in the A431 human epidermoid cancer cell line following heat shock and oxidative stress. Our results show that both a transient heat shock (20 min at 42 degrees C) and various oxidative stresses, including hydrogen peroxide, superoxide anion, hyperoxia and UVA exposure, induce a strong increase in clusterin mRNA levels as assessed by northern blot. Nuclear run-on analysis suggests that transcriptional activation is involved in inducing clusterin mRNA in response to heat shock. Using pulse-chase analysis of control and heat shocked cells, it is shown that clusterin mRNA is translated and secreted, thus resulting in increased extracellular levels of the protein following heat shock. To investigate the function of clusterin in response to these stresses, clusterin anti-sense transfectants that stably express virtually no clusterin at the mRNA and protein level were generated in A431 cells. These anti-sense transfectants are shown to be highly sensitive to apoptotic cell death induced by heat shock or oxidative stress compared with wild-type A431 cells or control transfectants. Taken together, our results show that clusterin gene expression is induced in response to heat shock and oxidative stress in human A431 cells, and confers cellular protection against heat shock and oxidative stress.  (+info)

Effect of targeted expression of clusterin in photoreceptor cells on retinal development and differentiation. (4/535)

Clusterin expression is increased in tissues undergoing apoptosis, including neurodegenerative retina, but the causal relationships remain to be clarified. To test the hypothesis that overexpression of clusterin could induce apoptosis in neurons, transgenic mice were generated in which rat clusterin transgene was expressed in photoreceptor cells under the transcriptional control of the human interphotoreceptor retinoid-binding protein (IRBP) promoter. Photoreceptor cell death in the resulting transgenic mice was examined by histology and TUNEL techniques. The expression of the clusterin transgene was confirmed by in situ hybridization in the photoreceptor cells, and results in a complex pattern of clusterin protein distribution in the retina. A reduction in apoptotic staining in the transgenic retinas was observed from birth to postnatal day 15. These results suggest that clusterin is not causally involved in apoptotic mechanisms of photoreceptor cell death, but may relate to cytoprotective functions.  (+info)

Nerve growth factor and epidermal growth factor stimulate clusterin gene expression in PC12 cells. (5/535)

Clusterin (apolipoprotein J) is an extracellular glycoprotein that might exert functions in development, cell death and lipid transport. Clusterin gene expression is elevated at sites of tissue remodelling, such as differentiation and apoptosis; however, the signals responsible for this regulation have not been identified. We use here the clusterin gene as a model system to examine expression in PC12 cells under the control of differentiation and proliferation signals produced by nerve growth factor (NGF) and by epidermal growth factor (EGF) respectively. NGF induced clusterin mRNA, which preceded neurite outgrowth typical of neuronal differentiation. EGF also activated the clusterin mRNA, demonstrating that both proliferation and differentiation signals regulate the gene. To localize NGF- and EGF-responsive elements we isolated the clusterin promoter and tested it in PC12 cell transfections. A 2.5 kb promoter fragment and two 1.5 and 0.3 kb deletion mutants were inducible by NGF and EGF. The contribution to this response of a conserved activator protein 1 (AP-1) motif located in the 0.3 kb fragment was analysed by mutagenesis. The mutant promoter was not inducible by NGF or EGF, which identifies the AP-1 motif as an element responding to both factors. Binding studies with PC12 nuclear extracts showed that AP-1 binds to this sequence in the clusterin promoter. These findings suggest that NGF and EGF, which give differential gene regulation in PC12 cells, resulting in neuronal differentiation and proliferation respectively, use the common Ras/extracellular signal-regulated kinase/AP-1 signalling pathway to activate clusterin expression.  (+info)

Clusterin (SGP-2) gene expression is cell cycle dependent in normal human dermal fibroblasts. (6/535)

In confluent human dermal fibroblasts brought to quiescence (G0) by serum starvation, the S phase peaked at 24 h after serum re-addiction and G2/M phase peaked at 36 h. This was confirmed by titration of h-gas1 mRNA (a marker of G0 phase) and histone H3 (a marker of S phase). Clusterin mRNA accumulation progressively increased in cells proceeding to confluence after seeding and to quiescence upon serum starvation, and peaked at around G0, in parallel with h-gas1 mRNA. At 6 h (roughly G1 phase) clusterin transcript formed a second peak, followed by a gradual decrease until 36 h. Correspondence of clusterin protein accumulation to its mRNA occurred solely with regard to the G0 peak but not to the second one. The possible meaning of the cell cycle related clusterin gene expression is discussed.  (+info)

Isolation of Ku70-binding proteins (KUBs). (7/535)

DNA-dependent protein kinase (DNA-PK) plays a critical role in resealing DNA double-stand breaks by non-homologous end joining. Aside from DNA-PK, XRCC4 and DNA ligase IV, other proteins which play a role(s) in this repair pathway remain unknown; DNA-PK contains a catalytic subunit (DNA-PKcs) and a DNA binding subunit (Ku70 and Ku80). We isolated Ku70-binding proteins (KUB1-KUB4) using yeast two-hybrid analyses. Sequence analyses revealed KUB1 to be apolipoprotein J (apoJ), also known as X-ray-inducible transcript 8 (XIP8), testosterone-repressed prostate message-2 (TRPM-2) and clusterin. KUB2 is Ku80. KUB3 and KUB4 are unknown, >10 kb trans-cripts. Interactions of apoJ/XIP8 or KUB3 with Ku70 were confirmed by co-immunoprecipitation analyses in MCF-7:WS8 breast cancer or IMR-90 normal lung fibroblast cells, respectively. The interaction of apoJ/XIP8 with Ku70 was confirmed by far-western analyses. Stable over-expression of full-length apoJ/XIP8 in MCF-7:WS8 caused decreased Ku70/Ku80 DNA end binding that was restored by apoJ/XIP8 monoclonal antibodies. The role of apoJ/XIP8 in ionizing radiation resistance/sensitivity is under investigation.  (+info)

Targeted disruption of the murine lecithin:cholesterol acyltransferase gene is associated with reductions in plasma paraoxonase and platelet-activating factor acetylhydrolase activities but not in apolipoprotein J concentration. (8/535)

Lecithin:cholesteryl acyltransferase (LCAT) deficiency resulting from targeted disruption of the Lcat gene in the mouse is associated with dramatic decreases in HDL concentration and the accumulation of nascent HDL in the plasma. We examined whether LCAT deficiency in mice is associated with a concomitant decrease in two antioxidative enzymes, paraoxonase (PON) and platelet-activating factor acetylhydrolase (PAF-AH). In control Lcat (+/+) mice both these enzymes are transported on HDL. Compared to Lcat (+/+) mice, HDL-cholesterol is reduced 94% and apoA-I, 90%, in Lcat (-/-) mice; this reduction in HDL is paralleled by a 71% decrease in PAF-AH activity and in a 58% decrease in PON activity. Apolipoprotein J (apoJ) levels, rather than being decreased, were significantly (P = 0.01) higher (36%) in Lcat (-/-) than in Lcat (+/+) mice, and the apo J/PON ratio was 3-fold greater in Lcat (-/-) than in Lcat (+/+) animals. Even though apolipoprotein A-I (apoA-I) concentration and PON activity were drastically reduced, there was no reduction in apoA-I and PON liver mRNA levels suggesting that post-transcriptional events are responsible for the reduction of plasma PON and apoA-I levels. Fast protein liquid chromatography (FPLC) revealed that in Lcat (+/+) mice both PON and PAF-AH activity is associated with large, apoA-I-containing HDL particles (9.7 nm by non-denaturing gradient gel electrophoresis) while in Lcat (-/-) mice both enzymes are associated with small 8.2 nm particles. We conclude that the concomitant reduction in HDL and apoA-I concentrations and PON and PAF-AH activities is best explained by rapid clearance of the small HDL particles found in LCAT deficiency.  (+info)

Clusterin is a protein that is widely expressed in many tissues and body fluids, including the tears, blood plasma, seminal fluid, milk, and cerebrospinal fluid. It is also known as apolipoprotein J or sulfated glycoprotein 2. Clusterin has diverse functions, including cell-cell communication, lipid transport, and protection against oxidative stress.

In the context of medicine and disease, clusterin has been studied for its potential role in several pathological processes, such as neurodegeneration, inflammation, cancer, and aging. In particular, clusterin has been implicated in the development and progression of various types of cancer, including prostate, breast, ovarian, and lung cancer. It is thought to contribute to tumor growth, invasion, and metastasis by promoting cell survival, angiogenesis, and resistance to chemotherapy.

Therefore, clusterin has been considered as a potential therapeutic target for cancer treatment, and several strategies have been developed to inhibit its expression or activity. However, more research is needed to fully understand the molecular mechanisms of clusterin in health and disease, and to translate these findings into effective clinical interventions.

Molecular chaperones are a group of proteins that assist in the proper folding and assembly of other protein molecules, helping them achieve their native conformation. They play a crucial role in preventing protein misfolding and aggregation, which can lead to the formation of toxic species associated with various neurodegenerative diseases. Molecular chaperones are also involved in protein transport across membranes, degradation of misfolded proteins, and protection of cells under stress conditions. Their function is generally non-catalytic and ATP-dependent, and they often interact with their client proteins in a transient manner.

Glycoproteins are complex proteins that contain oligosaccharide chains (glycans) covalently attached to their polypeptide backbone. These glycans are linked to the protein through asparagine residues (N-linked) or serine/threonine residues (O-linked). Glycoproteins play crucial roles in various biological processes, including cell recognition, cell-cell interactions, cell adhesion, and signal transduction. They are widely distributed in nature and can be found on the outer surface of cell membranes, in extracellular fluids, and as components of the extracellular matrix. The structure and composition of glycoproteins can vary significantly depending on their function and location within an organism.

Complement inactivator proteins are a group of regulatory proteins that help to control and limit the activation of the complement system, which is a part of the immune system. The complement system is a complex series of biochemical reactions that help to eliminate pathogens and damaged cells from the body. However, if not properly regulated, the complement system can also cause damage to healthy tissues and contribute to the development of various diseases.

Complement inactivator proteins work by inhibiting specific components of the complement system, preventing them from activating and causing an immune response. Some examples of complement inactivator proteins include:

1. C1 inhibitor (C1INH): This protein regulates the activation of the classical pathway of the complement system by inhibiting the C1 complex, which is a group of proteins that initiate this pathway.
2. Decay-accelerating factor (DAF or CD55): This protein regulates the activation of both the classical and alternative pathways of the complement system by accelerating the decay of the C3/C5 convertases, which are enzymes that activate the complement components C3 and C5.
3. Membrane cofactor protein (MCP or CD46): This protein regulates the activation of the alternative pathway of the complement system by serving as a cofactor for the cleavage and inactivation of C3b, a component of the C3 convertase.
4. Factor H: This protein also regulates the activation of the alternative pathway of the complement system by acting as a cofactor for the cleavage and inactivation of C3b, and by preventing the formation of the C3 convertase.

Deficiencies or dysfunction of complement inactivator proteins can lead to various diseases, including hereditary angioedema (C1INH deficiency), atypical hemolytic uremic syndrome (factor H deficiency or dysfunction), and age-related macular degeneration (complement component overactivation).

The rete testis is a network of tubules in the male reproductive system that serves as a passageway for sperm to travel from the seminiferous tubules, where sperm are produced, to the epididymis, where they mature. It is located in the mediastinum testis, which is the central part of the testicle.

The rete testis is made up of a series of interconnected tubules that are lined with simple cuboidal epithelial cells. These tubules merge to form larger ducts called efferent ductules, which then connect to the epididymis. The rete testis plays an important role in the transport and maturation of sperm, as well as in the regulation of fluid balance in the male reproductive system.

"Entrez Gene: clusterin". Koltai T (2014). "Clusterin: a key player in cancer chemoresistance and its inhibition". OncoTargets ... In humans, clusterin is encoded by the CLU gene on chromosome 8. CLU is a molecular chaperone responsible for aiding protein ... Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and ... Wei L, Xue T, Wang J, Chen B, Lei Y, Huang Y, Wang H, Xin X (Aug 2009). "Roles of clusterin in progression, chemoresistance and ...
Clusterin associated protein 1, also known as CLUAP1, is a human gene. Model organisms have been used in the study of CLUAP1 ... "Entrez Gene: clusterin associated protein 1". Retrieved 2011-08-30. "Radiography data for Cluap1". Wellcome Trust Sanger ...
... acts as an anti-cancer drug by binding to the mRNA initiation site of the clusterin gene, reducing clusterin protein ... The results found that the recommended dose of Custirsen was 640 mg, with maximum decrease of clusterin blood plasma occurring ... The anti-apoptotic activity of clusterin in aiding tumour growth is due to interactions with protein complexes. These include: ... Chi KN, Zoubeidi A, Gleave ME (December 2008). "Custirsen (OGX-011): a second-generation antisense inhibitor of clusterin for ...
Zhou Y, Li L, Hu L, Peng T (March 2011). "Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1) interacts with secretory clusterin". ...
Bajari TM, Strasser V, Nimpf J, Schneider WJ (2003). "A model for modulation of leptin activity by association with clusterin ...
Clusterin - Clusterin is a protein ubiquitously expressed in different cell lines. Secreted clusterin is involved in lipid ... The cells release clusterin as a response to cell stress. This is due to clusterin being able to protect the cell by reducing ... Kidney ischemia can be diagnosed by checking the levels of several biomarkers such as clusterin and cystatin C. While the ...
Clusterin is a heterodimeric protein that aids in the clearance of cellular debris and is involved with apoptosis. Clusterin ... Grogg, Karen L; Macon, William R; Kurtin, Paul J; Nascimento, Antonio G (2004). "A survey of clusterin and fascin expression in ... These cells are best visualized with immunostaining using the FDC markers CD21, CD35, R4/23, clusterin, and KiM4p. Marker ... Characteristically, FDCS have mircotubuloreticular structures (MTRS) and increased levels of intracellular clusterin. MTRS ...
"Identification of glycoprotein 330 as an endocytic receptor for apolipoprotein J/clusterin". The Journal of Biological ...
Vandepoele K, Staes K, Andries V, van Roy F (April 2010). "Chibby interacts with NBPF1 and clusterin, two candidate tumor ...
"Identification of human plasma proteins as major clients for the extracellular chaperone clusterin". The Journal of Biological ...
Antiapoptotic proteins, such as clusterin and survivin, are often expressed in cancer cells. Clusterin and survivin-targeting ... "Nucleotide-based therapies targeting clusterin chemosensitize human lung adenocarcinoma cells both in vitro and in vivo". ...
Apolipoprotein E4 and Clusterin to synaptic degeneration and has contributed to understanding of synapse degeneration in ... "Clusterin accumulates in synapses in Alzheimer's disease and is increased in apolipoprotein E4 carriers". Brain Communications ...
It appears together with clusterin and complement C3, markers of complement-mediated inflammatory reactions. Also Fibroblast ...
Lee KB, Jeon JH, Choi I, Kwon OY, Yu K, You KH (February 2008). "Clusterin, a novel modulator of TGF-beta signaling, is ...
Plasma clusterin and the risk of Alzheimer disease. JAMA. 2011 Apr 6;305(13):1322-6. Solouki AM, et al. A genome-wide ...
9 (3). Proteome Science , Full text , Development of reverse phase protein microarrays for the validation of clusterin, a mid- ...
Gleave's team was recognized in 2010 as the first in the world to develop an anti-clusterin agent. Richard D. Williams MD ...
2003). "Clusterin, an abundant serum factor, is a possible negative regulator of MT6-MMP/MMP-25 produced by neutrophils". J. ...
In these cases, the FD cells express FD-cell markers (e.g. CD21, CD23, CD35, clusterin, podoplanin, gamma-synuclein) and in >90 ...
These fluid phase complexes do not bind to cell membranes and are ultimately scavenged by clusterin and vitronectin, two ...
Clusterin, a soluble molecule with functions similar to CD59, forms a complex with Granzyme B and inhibits activation of ...
... is accompanied by significant changes in the levels of expression of polyamine metabolism regulatory genes and clusterin ( ...
Li, W.; Piecuch, P. (2010). "Multi-level Extension of the Cluster-In-Molecule Local Correlation Methodology: Merging Coupled- ...
Oak leaf cluster - In the Army, Air Force, and Space Force, the oak leaf cluster is worn to denote additional awards. 5/16 inch ...
... clusterin, and osteopontin (OPN), and in humans, insulin-like growth factor binding protein 7 (IGFBP7) as part of the ...
... the gene for clusterin CLU (programming language) Clu (Tron), fictional character from the Tron franchise Chartered Life ...
The following list gives an overview of the problems supported by HeuristicLab: Artificial Ant Classification Clusterin ...
... turnkey cluster-in-a-box solution that can be purchased, leased, or rented by smaller companies or individuals who want to ...
Among soluble inhibitors there are factor H, C1 inhibitor, C4b-binding protein, factor I, S protein or clusterin, the membrane- ...
For example, expression of CD56, MUC1 (also termed EMA for epithelial membrane antigen), and clusterin and strong uniform ...
"Entrez Gene: clusterin". Koltai T (2014). "Clusterin: a key player in cancer chemoresistance and its inhibition". OncoTargets ... In humans, clusterin is encoded by the CLU gene on chromosome 8. CLU is a molecular chaperone responsible for aiding protein ... Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and ... Wei L, Xue T, Wang J, Chen B, Lei Y, Huang Y, Wang H, Xin X (Aug 2009). "Roles of clusterin in progression, chemoresistance and ...
CLU (clusterin) is an evolutionarily-conserved, homeostatic, secreted glycoprotein, expressed prominently by mucosal epithelia ...
Human - Clusterin - for Western blotting - Antibodies. Product filter Clusterin molecule Acylation-Stimulating Protein ... Clusterin Connective Tissue Growth Factor CXCL12 Cystatin C Decorin Endostatin ENPP1 Epidermal Fatty Acid Binding Protein ERK1 ...
ApoJ/clusterin is a multifunctional protein highly expressed in the brain. The implication of ApoJ in β-amyloid (Aβ) ... Role of clusterin in the brain vascular clearance of amyloid-β. Proc Natl Acad Sci U S A. 2017;114(33):8681-2. ... ApoJ/clusterin is a multifunctional protein highly expressed in the brain. The implication of ApoJ in β-amyloid (Aβ) ... Clusterin promotes amyloid plaque formation and is critical for neuritic toxicity in a mouse model of Alzheimers disease. Proc ...
Clusterin. Clusterin, a plasma protein, plays an important role in the pathogenesis of AD. In one study, clusterin was ... Plasma clusterin and the risk of Alzheimer disease. JAMA. 2011 Apr 6. 305(13):1322-6. [QxMD MEDLINE Link]. ... Association of plasma clusterin concentration with severity, pathology, and progression in Alzheimer disease. Arch Gen ... 24] A study by Schrijvers et al notes that although plasma clusterin levels are significantly associated with baseline ...
Clusterin / Apolipoprotein J (APO-J) Antibody derived from Recombinant fragment (around aa150-300) of human CLU protein (exact ... Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly ... Be the first to review "Clusterin / Apolipoprotein J (APO-J) Antibody" Cancel reply. Your email address will not be published. ... Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg ...
The glycosylation of clusterin from human plasma was comprehensively analyzed and characterized using mass spectrometry (MS)- ... These results provide a novel and robust workflow suitable for rapid verification of specific clusterin glycoforms with utility ... which demonstrates the utility of clusterin isoforms as diagnostic and prognostic Alzheimers disease (AD) markers. ... More detailed structural characterization of clusterin glycopeptides was also performed, demonstrating the presence of ...
Clusterin/apolipoprotein J is independently associated with survival in patients with chronic heart failure. in: Journal of ... Clusterin/apolipoprotein J is independently associated with survival in patients with chronic heart failure. Journal of ... Clusterin/apolipoprotein J is independently associated with survival in patients with chronic heart failure. / Koller, Lorenz; ... BACKGROUND: Clusterin/apolipoprotein J (CLU) is a ubiquitous expressed glycoprotein with cytoprotective properties capable to ...
Clinical and epidemiological studies of older persons have implicated clusterin in Alzheimers disease (AD) pathogenesis. In ... Compared to mean clusterin, 1 and 2 standard deviation (SD) level increases in clusterin were associated with -2.1% (95% CI: - ... Clusterin was quantified using ELISA (mean 255± 31 ng/ml). Associations were assessed between clusterin and volumes of brain ... Negative non-linear associations between clusterin and MTLV were strongest for the left side: compared to mean clusterin, 1 and ...
... soluble clusterin (sCLU) or nuclear clusterin (nCLU) to normoxia, hypoxia and oxidative stress for 24 h. We then ... When overexpressed in murine neuronal cells (HN33), S100B and clusterin had neuroprotective effects when cells were exposed to ... Previous comparative transcriptomics of the visual cortex have revealed that S100B and clusterin (apolipoprotein J), two stress ... In order to investigate their potential roles, we transfected HN33 cells with soluble clusterin (sCLU), nucleus clusterin (nCLU ...
S3, e.g., clusterin, E3 ubiquitin-protein ligase, peroxiredoxin-1, glyceraldehyde-3-phosphate dehydrogenase, and 78-kDa glucose ... Clusterin enhances the formation of insoluble immune complexes. Biochem Biophys Res Commun. 1991;177(3):985-90. https://doi.org ...
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2014). Clusterin regulates β-amyloid toxicity via dickkopf-1-driven induction of the wnt-PCP-JNK pathway. Mol. Psychiatry 19, ... Several AD susceptibility genes (including APOE, TREM2, and Clusterin) are linked to aberrant Wnt signaling. For example, ApoE4 ... the AD-associated protein Clusterin (Harold et al., 2009; Lambert et al., 2009) is involved in Aß-driven Dkk1 expression, as ... whereas knock-down of Clusterin prevents induction of Dkk1 expression and protects against Aß neurotoxicity (Killick et al., ...
According to the ITGAM (Integrin alpha M chain) and CLU (Clusterin) were differentially expressed in serum exosomes among ... Tan J, Guo W, Yang S, Han D, Li H. The multiple roles and therapeutic potential of clusterin in non-small-cell lung cancer: a ... Finally, we considered further investigation of both the integrin alpha M chain (ITGAM) and Clusterin (CLU). Figure 4a and c ... Panico F, Rizzi F, Fabbri LM, Bettuzzi S, Luppi F. Clusterin (CLU) and lung cancer. Adv Cancer Res. 2009;105:63-76. ...
Researchers Discover Brain Protein Clusterin Disrupts Brain Synapses in Alzheimers By Being Patient , June 27th, 2019 ...
FDA granted Fast Track designation to OGX-011 for use in combination with docetaxel to treat progressive metastatic prostate cancer. The second-generation ...
It targets secreted clusterin for the inhibition of cancer-associated epithelial-to-mesenchymal transition (EMT). "It has shown ...
Find helpful learner reviews, feedback, and ratings for Kubernetes in AWS: Create Cluster in EKS in your own VPC from Coursera Project Network. Read stories and highlights from Coursera learners who completed Kubernetes in AWS: Create Cluster in EKS in your own VPC and wanted to share their experience.
"This is ten times more than any other known young clusterin the Milky Way. ...
Clusterin, an inhibitor of the complement cascade, was significantly more abundant in the marathoners blood than in the couch ... Clusterin by itself, even though administered outside the brain, was able to reduce brain inflammation in two different strains ... Wyss-Coray speculated that a drug that enhances or mimics clusterins binding to its receptors on brain endothelial cells might ... Further experiments showed that clusterin binds to receptors that abound on brain endothelial cells, the cells that line the ...
i,Materials and Methods,/i,. We compared expressions of AR, heat shock protein 27 (HSP27), clusterin (CLU), glucose-related ... Y. H. Youm, H. Yang, Y. D. Yoon, D. Y. Kim, C. Lee, and T. K. Yoo, "Doxazosin-induced clusterin expression and apoptosis in ... Clusterin acts as an antiapoptotic factor and plays an important role in resistance to chemotherapeutic drugs [35]. When ... Immunocytochemical analysis of HSP27, clusterin, GRP78, and c-FLIP at the es-LNCap, ls-LNCap, scr-ls-LNCap, and si-ls-LNCaP ...
The capacity the nuns demonstrated to coordinate projects and achieve results with the FUNDASEP program was key in the Kellogg Foundations decision to support the new initiative of the Sisters.. The municipality of Vallejuelo, located near the border with Haiti, is one of the poorest in the Dominican Republic. Nearly 90% of the population is made up of farming families. The lack of work opportunities has prompted the number of residents to fall each year. In 1992, the municipality was home to 40,000 people; today there are just 21,000. Half of them live in town, although they work in the surrounding countryside. Many who have left the region are now illegal immigrants in the United States.. The Education and Agriculture program aims to help slow down the exodus of the rural population by implementing education and sustainable agriculture initiatives capable of offering survival and development alternatives for the regions farmers.. The onion, abundant in Vallejuelo, is the municipalitys main ...
Bring festive vibes to your winter wonderland with this Grape cluster Christmas ornament. A teardrop cloche encases a cluster of green grape ornaments .Embelli
The CLUAP1 (clusterin-associated protein 1) protein plays a central role in photoreceptor ciliogenesis in the vertebrate retina ...
Regulation of Clusterin Gene Expression. Journal: Current Protein & Peptide Science. Volume: 19 Issue: 6 Year: 2018 Page: 612- ...
SBB NVMe cluster-in-a-box storage servers to deliver performing SAN/NAS solutions. May 7, 2021 , Press Release ...
Sophie Curtis from Techworld writes AWS HPC cloud services are available outside the US for the first time. In what may be the first in a series of deployments, Amazon Web Services has installed a cluster system at its EU West facility in Ireland.. Each Cluster Compute Eight Extra Large (cc2.8xl) instance includes a pair of Intel Xeon processors, 60.5GB of RAM, and 3.37TB of instance storage, according to AWS. Each processor has 8 cores and hyperthreading is enabled, so users can execute up to 32 threads in parallel.. ...
Below are the links to the news stories that Clyde has been in. Click on the link to read the news story.. ...
  • Clusterin (apolipoprotein J) is a 75-80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. (wikipedia.org)
  • BACKGROUND: Clusterin/apolipoprotein J (CLU) is a ubiquitous expressed glycoprotein with cytoprotective properties capable to prevent myocardial injury in experimental studies. (kl.ac.at)
  • Previous comparative transcriptomics of the visual cortex have revealed that S100B and clusterin (apolipoprotein J), two stress proteins that are involved in neurological disorders characterized by hypoxic conditions, have a remarkably high expression in hooded seals compared to ferrets. (biomedcentral.com)
  • Formalin-fixed, paraffin-embedded human adrenal gland stained with Clusterin / APOJ Mouse Monoclonal Antibody (CLU/4740). (neobiotechnologies.com)
  • Two independent genome-wide association studies found a statistical association between a SNP within the clusterin gene and the risk of having Alzheimer's disease. (wikipedia.org)
  • Further studies have suggested that people who already have Alzheimer's disease have more clusterin in their blood, and that clusterin levels in blood correlate with faster cognitive decline in individuals with Alzheimer's disease, but have not found that clusterin levels predicted the onset of Alzheimer's disease. (wikipedia.org)
  • Glycosylation of Human Plasma Clusterin Yields a Novel Candidate Biomarker of Alzheimer's Disease. (ox.ac.uk)
  • Eight β64N glycoforms are significantly reduced in patients with high atrophy compared with those with low atrophy, which demonstrates the utility of clusterin isoforms as diagnostic and prognostic Alzheimer's disease (AD) markers. (ox.ac.uk)
  • Clinical and epidemiological studies of older persons have implicated clusterin in Alzheimer's disease (AD) pathogenesis. (kaiserpermanente.org)
  • Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we have determined the differences in the glycoforms associated at each of the different glycosylation sites in plasma clusterin obtained from subjects of low hippocampal atrophy (n = 13) and high hippocampal atrophy (n = 14). (ox.ac.uk)
  • In the context of identifying early biomarkers of risk, we examined associations of plasma clusterin and characteristics of AD in middle-aged individuals from the community. (kaiserpermanente.org)
  • In middle-aged adults unselected for AD, plasma clusterin was associated with lower volume of the entorhinal cortex, an area that atrophies early in AD. (kaiserpermanente.org)
  • ApoJ/clusterin is a multifunctional protein highly expressed in the brain. (biomedcentral.com)
  • In a comparative transcriptomics analysis, the calcium-binding protein S100B and the molecular chaperone clusterin (CLU) emerged as highly expressed in the hooded seal visual cortex, when compared to the ferret ( Mustela putorius furo ). (biomedcentral.com)
  • We compared expressions of AR, heat shock protein 27 (HSP27), clusterin (CLU), glucose-related protein 78 (GRP78), and cellular FLICE-like inhibitory protein (c-FLIP) and their genes between es-LNCaP (less than 33 times subcultured, L-33), ls-LNCaP (over 81 times subcultured, H-81), and si-LNCaP (AR siRNA transfected ls-LNCaP) by Western blotting and RT-PCR. (hindawi.com)
  • These results provide a novel and robust workflow suitable for rapid verification of specific clusterin glycoforms with utility as AD biomarkers. (ox.ac.uk)
  • Specific glycosylated peptides of clusterin are found associated with hippocampal atrophy. (ox.ac.uk)
  • Associations were assessed between clusterin and volumes of brain regions known to atrophy in early AD, including entorhinal cortex (ECV), hippocampus (HV), and medial temporal lobe (MTLV) volumes (cm3). (kaiserpermanente.org)
  • In humans, clusterin is encoded by the CLU gene on chromosome 8. (wikipedia.org)
  • CLU (clusterin) is an evolutionarily-conserved, homeostatic, secreted glycoprotein, expressed prominently by mucosal epithelia at fluid-tissue interfaces and found in all bodily fluids. (sbir.gov)
  • The glycosylation of clusterin from human plasma was comprehensively analyzed and characterized using mass spectrometry (MS)-based glycoproteomics analysis. (ox.ac.uk)
  • In multivariable models, higher clusterin had a negative non-linear association with ECV (combined left and right hemispheres), and this association was influenced by the highest clusterin levels. (kaiserpermanente.org)
  • According to the ITGAM (Integrin alpha M chain) and CLU (Clusterin) were differentially expressed in serum exosomes among different groups as well as tumor tissues and adjacent tissues. (biomedcentral.com)
  • There were no significant associations between clusterin and brain structures affected in later AD. (kaiserpermanente.org)
  • Just as a scabbard shields from razor sharp edges, the extracellular chaperone clusterin may ensheathe and sequester lethal forms of Aβ, suggests a Nature Structural and Molecular Biology paper published online December 18. (alzforum.org)
  • The extracellular chaperone clusterin sequesters oligomeric forms of the amyloid-β(1-40) peptide. (nih.gov)
  • Single Nucleotide Polymorphism rs11136000 of CLU Gene (Clusterin, ApoJ) and the Risk of Late-Onset Alzheimer's Disease in a Central European Population. (cdc.gov)
  • Notably, it is not yet clear whether this would be neuroprotective or harmful, but the finding provides molecular support for the genetic linkage between clusterin (currently number three in AlzGene's Top Results ) and Alzheimer's disease. (alzforum.org)
  • Recent genetic and proteomic studies demonstrate that clusterin/apolipoprotein-J is associated with risk, pathology, and progression of Alzheimer's disease (AD). (kcl.ac.uk)
  • Water-maze testing showed an improved cognitive function of the Tg6799 mice treated with clusterin. (nih.gov)
  • They report that clusterin binds and stabilizes Aβ oligomers of all sizes. (alzforum.org)
  • Some studies suggest that, like ApoE, clusterin modulates clearance of Aβ or other misfolded proteins from the brain (see, e.g. (alzforum.org)
  • 8. Targeting the cytoprotective chaperone, clusterin, for treatment of advanced cancer. (nih.gov)
  • Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE . (bvsalud.org)
  • 12. mda-7/IL-24 differentially regulates soluble and nuclear clusterin in prostate cancer. (nih.gov)
  • In the combined autopsy sample of AD and control cases, representing a range of severity in AD pathology, we observed a significant association between clusterin concentration in the plasma and that in the superior temporal gyrus. (kcl.ac.uk)
  • Furthermore, peripheral concentration of clusterin also appears to reflect its concentration within brain regions vulnerable to AD pathology. (kcl.ac.uk)
  • Immunocytochemistry and quantitative analysis revealed that intraventricular (icv) administration of clusterin peptide in Tg6799 mouse reduced Aβ plaques as well the severity of cerebral amyloid angiopathy. (nih.gov)
  • 2. Clusterin confers resistance to TNF-alpha-induced apoptosis in breast cancer cells through NF-kappaB activation and Bcl-2 overexpression. (nih.gov)
  • 16. Non-secreted clusterin isoforms are translated in rare amounts from distinct human mRNA variants and do not affect Bax-mediated apoptosis or the NF-κB signaling pathway. (nih.gov)
  • 20. Inhibition of NF-kappaB-dependent Bcl-xL expression by clusterin promotes albumin-induced tubular cell apoptosis. (nih.gov)
  • Glicoproteína heterodimérica, muy estable, que se expresa en muy diversos trastornos fisiológicos graves, como el CÁNCER, la APOPTOSIS y algunas TRASTORNOS NEUROLÓGICOS. (bvsalud.org)
  • Plasma levels of clusterin were measured at baseline in 60 individuals with prevalent AD, a random subcohort of 926 participants, and in an additional 156 participants who were diagnosed with AD during follow-up until January 2007. (medscape.com)
  • Plasma clusterin levels were not related to the risk for incident AD during the total follow-up or within 3 years of baseline [examination]. (medscape.com)
  • The investigators concluded that plasma clusterin levels were signficantly associated with the baseline prevalence and severity of AD but not with the incidence of AD. (medscape.com)
  • Clusterin concentration was assayed by ELISA in plasma samples collected within a year of the baseline MRI. (kcl.ac.uk)
  • Within the MCI but not control group, higher baseline concentration of plasma clusterin was associated with slower rates of brain atrophy in these regions. (kcl.ac.uk)
  • It is important to note that although plasma clusterin levels are significantly associated with baseline prevalence and severity of AD , they are not related to the risk for AD. (medscape.com)
  • A team from Brigham and Women's Hospital in Boston and from Thermo Fischer Scientific have found that the synovial proteins clusterin (which removes debris) and lubricin (which reduces wear in cartilage) both correlate with joint space narrowing over the course of 30 months. (hcplive.com)
  • In this study, we investigated whether this clusterin peptide also affected (Aβ) deposition in AD transgenic mouse. (nih.gov)
  • These results suggest that icv infusion of clusterin peptide D-[113-122] offers a promising therapeutic approach to reduce Aβ deposition as well as CAA. (nih.gov)
  • Although all the experiments were performed in vitro, Narayan and colleagues note that Aβ and clusterin are present in human cerebrospinal fluid (CSF) at concentrations that would allow these complexes to form. (alzforum.org)
  • Identification of a novel bile marker clusterin and a public online prediction platform based on deep learning for cholangiocarcinoma. (bvsalud.org)
  • The associations of plasma clusterin concentration with rates of change in brain volume were significantly different between MCI and control groups in several volumes including whole brain, ventricular CSF, temporal gray matter as well as parahippocampal, superior temporal and cingulate gyri. (kcl.ac.uk)
  • The results of proteomics analysis and clinical specimen verification showed that bile clusterin (CLU) was significantly higher in CCA body fluids . (bvsalud.org)
  • Recently, clusterin peptide D-[113-122] was shown to mimic clusterin's function and exerted therapeutic effect in atherosclerosis. (nih.gov)
  • 13. Identification of clusterin domain involved in NF-kappaB pathway regulation. (nih.gov)
  • Clusterin added in equimolar amounts with monomeric Aβ prevented oligomer formation, the authors report. (alzforum.org)
  • The addition of human clusterin changed the behavior of the solution, however. (alzforum.org)
  • tential association between clusterin and kidney injury. (deu.edu.tr)
  • 1. Clusterin facilitates COMMD1 and I-kappaB degradation to enhance NF-kappaB activity in prostate cancer cells. (nih.gov)
  • 3. Clusterin knockdown using the antisense oligonucleotide OGX-011 re-sensitizes docetaxel-refractory prostate cancer PC-3 cells to chemotherapy. (nih.gov)
  • Researchers from The Netherlands have published a case cohort study nested within the Rotterdam study evaluating the potential of plasma clusterin as a biomarker of the presence, severity, and risk for Alzheimer disease (AD). (medscape.com)
  • One cannot do in vivo the type of beautiful in-vitro assessment of oligomers as was done in the Narayan paper, so we do not know what the status of oligomers is in the presence or absence of clusterin in vivo," Holtzman wrote to ARF. (alzforum.org)
  • Western-blot revealed an increased level of LRP-2 after clusterin peptide treatment. (nih.gov)
  • 19. Induction of clusterin by AKT--role in cytoprotection against docetaxel in prostate tumor cells. (nih.gov)