A highly conserved heterodimeric glycoprotein that is differentially expressed during many severe physiological disturbance states such as CANCER; APOPTOSIS; and various NEUROLOGICAL DISORDERS. Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE.
A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.
Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.
Serum proteins that negatively regulate the cascade process of COMPLEMENT ACTIVATION. Uncontrolled complement activation and resulting cell lysis is potentially dangerous for the host. The complement system is tightly regulated by inactivators that accelerate the decay of intermediates and certain cell surface receptors.
The network of channels formed at the termination of the straight SEMINIFEROUS TUBULES in the mediastinum testis. Rete testis channels drain into the efferent ductules that pass into the caput EPIDIDYMIS.

Recovery following relief of unilateral ureteral obstruction in the neonatal rat. (1/535)

BACKGROUND: Obstructive nephropathy is a primary cause of renal insufficiency in infants and children. This study was designed to distinguish the reversible and irreversible cellular consequences of temporary unilateral ureteral obstruction (UUO) on the developing kidney. METHODS: Rats were subjected to UUO or sham operation in the first 48 hours of life, and the obstruction was removed five days later (or was left in place). Kidneys were removed for study 14 or 28 days later. In additional groups, kidneys were removed at the end of five days of obstruction. Immunoreactive distribution of renin was determined in arterioles, and the distribution of epidermal growth factor, transforming growth factor-beta1, clusterin, vimentin, and alpha-smooth muscle actin was determined in tubules and/or interstitium. The number of glomeruli, glomerular maturation, tubular atrophy, and interstitial collagen deposition was determined by morphometry. Renal cellular proliferation and apoptosis were measured by proliferating cell nuclear antigen and the TdT uridine-nick-end-label technique, respectively. The glomerular filtration rate was measured by inulin clearance. RESULTS: Renal microvascular renin maintained a fetal distribution with persistent UUO; this was partially reversed by the relief of obstruction. Although glomerular maturation was also delayed and glomerular volume was reduced by UUO, the relief of obstruction prevented the reduction in glomerular volume. Although relief of obstruction did not reverse a 40% reduction in the number of nephrons, the glomerular filtration rate of the postobstructed kidney was normal. The relief of obstruction did not improve tubular cell proliferation and only partially reduced apoptosis induced by UUO. This was associated with a persistent reduction in the tubular epidermal growth factor. In addition, the relief of obstruction reduced but did not normalize tubular expression of transforming growth factor-beta1, clusterin, and vimentin, all of which are evidence of persistent tubular injury. The relief of obstruction significantly reduced interstitial fibrosis and expression of alpha-smooth muscle actin by interstitial fibroblasts, but not to normal levels. CONCLUSIONS: The relief of obstruction in the neonatal rat attenuates, but does not reverse, renal vascular, glomerular, tubular, and interstitial injury resulting from five days of UUO. Hyperfiltration by remaining nephrons and residual tubulointerstitial injury in the postobstructed kidney are likely to lead to deterioration of renal function later in life.  (+info)

Clusterin has chaperone-like activity similar to that of small heat shock proteins. (2/535)

Clusterin is a highly conserved protein which is expressed at increased levels by many cell types in response to a broad variety of stress conditions. A genuine physiological function for clusterin has not yet been established. The results presented here demonstrate for the first time that clusterin has chaperone-like activity. At physiological concentrations, clusterin potently protected glutathione S-transferase and catalase from heat-induced precipitation and alpha-lactalbumin and bovine serum albumin from precipitation induced by reduction with dithiothreitol. Enzyme-linked immunosorbent assay data showed that clusterin bound preferentially to heat-stressed glutathione S-transferase and to dithiothreitol-treated bovine serum albumin and alpha-lactalbumin. Size exclusion chromatography and SDS-polyacrylamide gel electrophoresis analyses showed that clusterin formed high molecular weight complexes (HMW) with all four proteins tested. Small heat shock proteins (sHSP) also act in this way to prevent protein precipitation and protect cells from heat and other stresses. The stoichiometric subunit molar ratios of clusterin:stressed protein during formation of HMW complexes (which for the four proteins tested ranged from 1.0:1.3 to 1.0:11) is less than the reported ratios for sHSP-mediated formation of HMW complexes (1.0:1.0 or greater), indicating that clusterin is a very efficient chaperone. Our results suggest that clusterin may play a sHSP-like role in cytoprotection.  (+info)

Clusterin gene expression mediates resistance to apoptotic cell death induced by heat shock and oxidative stress. (3/535)

Clusterin is a widely expressed, well conserved, secreted glycoprotein, which is highly induced in tissues regressing as a consequence of apoptotic cell death in vivo. It has recently been shown that clusterin expression is only confined to surviving cells following the induction of apoptosis in vitro, suggesting that it is involved in cell survival rather than death. In the hypothesis that clusterin may be implicated in cellular responses to stress, clusterin gene expression was analyzed in the A431 human epidermoid cancer cell line following heat shock and oxidative stress. Our results show that both a transient heat shock (20 min at 42 degrees C) and various oxidative stresses, including hydrogen peroxide, superoxide anion, hyperoxia and UVA exposure, induce a strong increase in clusterin mRNA levels as assessed by northern blot. Nuclear run-on analysis suggests that transcriptional activation is involved in inducing clusterin mRNA in response to heat shock. Using pulse-chase analysis of control and heat shocked cells, it is shown that clusterin mRNA is translated and secreted, thus resulting in increased extracellular levels of the protein following heat shock. To investigate the function of clusterin in response to these stresses, clusterin anti-sense transfectants that stably express virtually no clusterin at the mRNA and protein level were generated in A431 cells. These anti-sense transfectants are shown to be highly sensitive to apoptotic cell death induced by heat shock or oxidative stress compared with wild-type A431 cells or control transfectants. Taken together, our results show that clusterin gene expression is induced in response to heat shock and oxidative stress in human A431 cells, and confers cellular protection against heat shock and oxidative stress.  (+info)

Effect of targeted expression of clusterin in photoreceptor cells on retinal development and differentiation. (4/535)

Clusterin expression is increased in tissues undergoing apoptosis, including neurodegenerative retina, but the causal relationships remain to be clarified. To test the hypothesis that overexpression of clusterin could induce apoptosis in neurons, transgenic mice were generated in which rat clusterin transgene was expressed in photoreceptor cells under the transcriptional control of the human interphotoreceptor retinoid-binding protein (IRBP) promoter. Photoreceptor cell death in the resulting transgenic mice was examined by histology and TUNEL techniques. The expression of the clusterin transgene was confirmed by in situ hybridization in the photoreceptor cells, and results in a complex pattern of clusterin protein distribution in the retina. A reduction in apoptotic staining in the transgenic retinas was observed from birth to postnatal day 15. These results suggest that clusterin is not causally involved in apoptotic mechanisms of photoreceptor cell death, but may relate to cytoprotective functions.  (+info)

Nerve growth factor and epidermal growth factor stimulate clusterin gene expression in PC12 cells. (5/535)

Clusterin (apolipoprotein J) is an extracellular glycoprotein that might exert functions in development, cell death and lipid transport. Clusterin gene expression is elevated at sites of tissue remodelling, such as differentiation and apoptosis; however, the signals responsible for this regulation have not been identified. We use here the clusterin gene as a model system to examine expression in PC12 cells under the control of differentiation and proliferation signals produced by nerve growth factor (NGF) and by epidermal growth factor (EGF) respectively. NGF induced clusterin mRNA, which preceded neurite outgrowth typical of neuronal differentiation. EGF also activated the clusterin mRNA, demonstrating that both proliferation and differentiation signals regulate the gene. To localize NGF- and EGF-responsive elements we isolated the clusterin promoter and tested it in PC12 cell transfections. A 2.5 kb promoter fragment and two 1.5 and 0.3 kb deletion mutants were inducible by NGF and EGF. The contribution to this response of a conserved activator protein 1 (AP-1) motif located in the 0.3 kb fragment was analysed by mutagenesis. The mutant promoter was not inducible by NGF or EGF, which identifies the AP-1 motif as an element responding to both factors. Binding studies with PC12 nuclear extracts showed that AP-1 binds to this sequence in the clusterin promoter. These findings suggest that NGF and EGF, which give differential gene regulation in PC12 cells, resulting in neuronal differentiation and proliferation respectively, use the common Ras/extracellular signal-regulated kinase/AP-1 signalling pathway to activate clusterin expression.  (+info)

Clusterin (SGP-2) gene expression is cell cycle dependent in normal human dermal fibroblasts. (6/535)

In confluent human dermal fibroblasts brought to quiescence (G0) by serum starvation, the S phase peaked at 24 h after serum re-addiction and G2/M phase peaked at 36 h. This was confirmed by titration of h-gas1 mRNA (a marker of G0 phase) and histone H3 (a marker of S phase). Clusterin mRNA accumulation progressively increased in cells proceeding to confluence after seeding and to quiescence upon serum starvation, and peaked at around G0, in parallel with h-gas1 mRNA. At 6 h (roughly G1 phase) clusterin transcript formed a second peak, followed by a gradual decrease until 36 h. Correspondence of clusterin protein accumulation to its mRNA occurred solely with regard to the G0 peak but not to the second one. The possible meaning of the cell cycle related clusterin gene expression is discussed.  (+info)

Isolation of Ku70-binding proteins (KUBs). (7/535)

DNA-dependent protein kinase (DNA-PK) plays a critical role in resealing DNA double-stand breaks by non-homologous end joining. Aside from DNA-PK, XRCC4 and DNA ligase IV, other proteins which play a role(s) in this repair pathway remain unknown; DNA-PK contains a catalytic subunit (DNA-PKcs) and a DNA binding subunit (Ku70 and Ku80). We isolated Ku70-binding proteins (KUB1-KUB4) using yeast two-hybrid analyses. Sequence analyses revealed KUB1 to be apolipoprotein J (apoJ), also known as X-ray-inducible transcript 8 (XIP8), testosterone-repressed prostate message-2 (TRPM-2) and clusterin. KUB2 is Ku80. KUB3 and KUB4 are unknown, >10 kb trans-cripts. Interactions of apoJ/XIP8 or KUB3 with Ku70 were confirmed by co-immunoprecipitation analyses in MCF-7:WS8 breast cancer or IMR-90 normal lung fibroblast cells, respectively. The interaction of apoJ/XIP8 with Ku70 was confirmed by far-western analyses. Stable over-expression of full-length apoJ/XIP8 in MCF-7:WS8 caused decreased Ku70/Ku80 DNA end binding that was restored by apoJ/XIP8 monoclonal antibodies. The role of apoJ/XIP8 in ionizing radiation resistance/sensitivity is under investigation.  (+info)

Targeted disruption of the murine lecithin:cholesterol acyltransferase gene is associated with reductions in plasma paraoxonase and platelet-activating factor acetylhydrolase activities but not in apolipoprotein J concentration. (8/535)

Lecithin:cholesteryl acyltransferase (LCAT) deficiency resulting from targeted disruption of the Lcat gene in the mouse is associated with dramatic decreases in HDL concentration and the accumulation of nascent HDL in the plasma. We examined whether LCAT deficiency in mice is associated with a concomitant decrease in two antioxidative enzymes, paraoxonase (PON) and platelet-activating factor acetylhydrolase (PAF-AH). In control Lcat (+/+) mice both these enzymes are transported on HDL. Compared to Lcat (+/+) mice, HDL-cholesterol is reduced 94% and apoA-I, 90%, in Lcat (-/-) mice; this reduction in HDL is paralleled by a 71% decrease in PAF-AH activity and in a 58% decrease in PON activity. Apolipoprotein J (apoJ) levels, rather than being decreased, were significantly (P = 0.01) higher (36%) in Lcat (-/-) than in Lcat (+/+) mice, and the apo J/PON ratio was 3-fold greater in Lcat (-/-) than in Lcat (+/+) animals. Even though apolipoprotein A-I (apoA-I) concentration and PON activity were drastically reduced, there was no reduction in apoA-I and PON liver mRNA levels suggesting that post-transcriptional events are responsible for the reduction of plasma PON and apoA-I levels. Fast protein liquid chromatography (FPLC) revealed that in Lcat (+/+) mice both PON and PAF-AH activity is associated with large, apoA-I-containing HDL particles (9.7 nm by non-denaturing gradient gel electrophoresis) while in Lcat (-/-) mice both enzymes are associated with small 8.2 nm particles. We conclude that the concomitant reduction in HDL and apoA-I concentrations and PON and PAF-AH activities is best explained by rapid clearance of the small HDL particles found in LCAT deficiency.  (+info)

Both isoforms of the hepatitis delta antigen (HDAg) of hepatitis delta virus (HDV) are highly associated with virus proliferation and may act as co-activators of cellular gene expression. Human hepatocellular carcinoma (HCC) cell line Huh7, which stably expresses HDAgs, was differentially screened and the results showed that clusterin gene expression was enhanced. The mechanisms for HDAg-mediated clusterin gene upregulation were investigated. Expression of HDAgs was associated with enhanced histone H3 acetylation within the clusterin promoter in a chromatin immunoprecipitation assay. Transient transfection of HDAg-expressing plasmids into Huh7 cells also enhanced clusterin expression and histone acetylation. Furthermore, HDV replication was associated with histone hyperacetylation and clusterin induction. The effect of increased clusterin expression was determined by a chemosensitivity assay with adriamycin treatment. These data indicated that HDV-induced clusterin protein increases cell survival
Title:Secretory Clusterin: A Promising Target for Chemoresistance of Hepatocellular Carcinoma. VOLUME: 20 ISSUE: 12. Author(s):Jie Zhang, Mengna Wu, Yuqing Xu, Qianqian Song and Wenjie Zheng*. Affiliation:Department of Chemotherapy, Affiliated Hospital of Nantong University, 20 Xisi Road, 226001 Nantong, Jiangsu, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, 20 Xisi Road, 226001 Nantong, Jiangsu, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, 20 Xisi Road, 226001 Nantong, Jiangsu, Department of Radiology, Wake Forest School of Medicine, One Medical Center Boulevard, Winston-Salem, 27157 NC, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, 20 Xisi Road, 226001 Nantong, Jiangsu. Keywords:Hepatocellular carcinoma, secretory clusterin, drug resistance, molecular target, cancer, sCLU.. Abstract:Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths worldwide. Chemoresistance ...
TY - JOUR. T1 - Interaction of complement and clusterin in renal injury. AU - Correa-Rotter, Ricardo. AU - Hostetter, Thomas H.. AU - Nath, Karl A. AU - Manivel, J. Carlos. AU - Rosenberg, Mark E.. PY - 1992/11. Y1 - 1992/11. N2 - Clusterin is a heterodimeric glycoprotein that has been associated with such diverse biologic functions as reproduction, cell regression, cell aggregation, and regulation of the cytolytic activity of the membrane attack complex of complement. Clusterin is a component of glomerular immune deposits in the kidney, and increased Clusterin expression occurs in a number of renal injury states. To further explore the interaction between Clusterin and complement, the requirement for an intact complement system for renal Clusterin induction in an acute (folic acid nephropathy) and a chronic (subtotal renal ablation) model of renal injury was examined. After it was first demonstrated that folic acid increased renal clusterin mRNA in the rat, a species in which renal clusterin ...
Background: Clusterin is a cytoprotective chaperone protein that promotes cell survival and confers broad-spectrum treatment resistance. OGX-011 is a 2′-methoxyethyl modified phosphorothioate antisense oligonucleotide that is complementary to clusterin mRNA and has been reported to inhibit clusterin expression and enhance drug efficacy in xenograft models. The primary objective of this clinical study was to determine a biologically effective dose of OGX-011 that would inhibit clusterin expression in human cancer. Methods: Subjects (n = 25) with localized prostate cancer with high-risk features who were candidates for prostatectomy were treated with OGX-011 by 2-hour intravenous infusion on days 1, 3, and 5 and then weekly from days 8-29 combined with androgen blockade starting on day 1; prostatectomy was performed on days 30-36. Six different doses were tested, from 40 to 640 mg. OGX-011 plasma and prostate tissue concentrations were measured by an enzyme-linked immunosorbent assay method, and ...
Purpose: The clusterin gene encodes a multi-functional protein that has been identified in different tissues, including a number of different eye tissues, primarily in the mouse and to a much lesser extent in humans. Clusterin has been implicated in a number of cellular processes such as lipid transport, membrane integrity, apoptosis, and neurodegeneration, all of which could be important to the biology of the eye. In the current communication, we provide data that confirms the expression of clusterin in a number of different human eye tissues and establishes the expression profile of this gene in monkey derived eye tissues. The issue that we sought to examine is whether a broad profile of clusterin expression in the eye is consistent in primates (monkey and human). Methods: The majority of our study was done using monkey eye tissues. Where possible, we have used human tissues in order to confirm published findings. Northern and western analysis was performed using tissues derived from monkey eyes. In
Antiestrogens represent the first line of therapy in the treatment of estrogen receptor-positive (ER+) breast cancer patients. Unfortunately, up to 40% of patients develop resistance associated with progression and frequently die for metastatic breast cancer. The molecular events leading to pharmacological resistance are not completely understood. We attempted to verify in an experimental model the role of cytoplasmic clusterin (CLU), a cytoprotective protein found to be up-regulated in antiestrogen-resistant patients, following neoadjuvant treatment with toremifene. The role of cytoplasmic clusterin in modulating response to two antiestrogens (toremifene and tamoxifen) was studied in two ER+ anti-estrogen-sensitive cell lines (MCF-7, 734B) and one ER+ antiestrogen-resistant cell line (T47D) using siRNA strategy. Resistant cells were characterised by higher levels of cytoplasmic clusterin than sensitive cells, and antiestrogen treatments up-regulated clusterin levels in both sensitive and ...
Apolipoprotein J Antibody functions as a secreted chaperone that prevents aggregation of nonnative proteins. It prevents stress-induced aggregation of blood plasma proteins and inhibits the formations of amyloid fibrils. Apolipoprotein J does not require ATP or refold proteins by itself. It maintains partially unfolded proteins in a state for subsequent refolding by other chaperones. It is shown to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Binding to cell surface receptors it triggers internalization of chaperone-client complex and subsequent lysosomal or proteasomal degradation. It modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis while mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Anti-Apolipoprotein J Antibody is useful for researchers interested in the immune system, Ubiquitin pathways, and cardiovascular research.
Clusterin is a chaperone protein that is synthesized in response to cellular stress. It often appears in cells undergoing apoptosis - both as part of developmental programmes such as tissue involution and in numerous pathological conditions - but has also been reported to confer resistance to apoptosis in some cases. Whether it is a pro-survival or a pro-death molecule is therefore unclear. Laure Debure and co-workers have examined the effects of clusterin expression in COS-7 cells (see p. 3109). They find that clusterin can accumulate in juxtanuclear aggregates that exhibit features characteristic of aggresomes (inclusion bodies thought to prevent misfolded proteins spreading throughout the cell). They have a vimentin cage, for example, and can be disrupted by treatment with the chaperone Hsp70 or microtubule-depolymerising drugs. The authors also observe, however, that clusterin expression disrupts mitochondria and induces apoptosis through the mitochondrial pathway - this can be prevented by ...
Clusterin (CLU; also known as apolipoprotein J, ApoJ) is a protein of inconstant structure known to be involved in diverse processes inside and outside of brain cells. CLU can act as a protein chaperon or protein solubilizer, lipid transporter as well as redox sensor and be anti- or proapoptotic, depending on context. Primary structure of CLU is encoded by CLU gene which contains single nucleotide polymorphisms (SNPs) associated with the risk of late-onset Alzheimers disease (LOAD). Studying a sample of Czech population and using the case-control association approach we identified C allele of the SNP rs11136000 as conferring a reduced risk of LOAD, more so in females than in males. Additionally, data from two smaller subsets of the population sample suggested a possible association of rs11136000 with diabetes mellitus. In a parallel study, we found no association between rs11136000 and mild cognitive impairment (MCI). Our findings on rs11136000 and LOAD contradict those of some previous ...
Clusterin was originally described as a major glycoprotein synthesized in the male reproductive systems of the ram and rat.1,2 Since then it has been identified in a wide range of biological fluids and tissues in many species.1,3-9 Identification of rat clusterin messenger ribonucleic acid (mRNA) to TRPM-2 (testosterone repressed prostatic message 2),10-12 a transcript found to be prevalent in vivo in involuting tissues whether induced in experimental models or naturally during development of the embryo, raised the question of a possible involvement of clusterin in programmed cell death.13,14 Reports by several independent groups of researchers on the upregulation of the clusterin gene in brains of hamsters infected with the scrapie agent15 and of humans afflicted with Alzheimers disease (AD),16 epilepsy,17 or gliomas,17 as well as in the degenerating human retina18 gave support to the apoptosis hypothesis and generated strong interest in the role of clusterin in the central nervous system ...
Immature motoneurons are highly susceptible to degeneration following axon injury. The response of perineuronal glia to axon injury may significantly influence neuronal survival and axon regeneration. We have examined the central reactions to neonatal facial nerve transection with emphasis on the expression of complement component C3 (C3) and the multifunctional apolipoprotein J (ApoJ). Axotomy was performed on one-day-old rats. Animals were perfused from eight hours to two weeks after the lesion. The astroglial marker, glial fibrillary acidic protein (GFAP) was increased from one day and the microglial marker OX-42 from two days after injury. ApoJ immunoreactivity was increased in axotomized neuronal perikarya and astroglial cells from one day postaxotomy, but no C3 immunoreactive profiles were found at any postoperative survival time. Cell proliferation as judged by bromodeoxyuridine labeling and immunoreactivity for the cyclin Ki-67 antigen (antibody MIB5) occurred only at two days after ...
TY - JOUR. T1 - Clusterin and DNA repair. T2 - A new function in cancer for a key player in apoptosis and cell cycle control. AU - Shannan, B.. AU - Seifert, M.. AU - Boothman, D. A.. AU - Tilgen, W.. AU - Reichrath, J.. PY - 2006/9/1. Y1 - 2006/9/1. N2 - The glycoprotein clusterin (CLU), has two known isoforms generated in human cells. A nuclear form of CLU protein (nCLU) is pro-apoptotic, while a secretory form (sCLU) is pro-survival. Both forms are implicated in various cell functions, including DNA repair, cell cycle regulation, and apoptotic cell death. CLU expression has been associated with tumorigenesis and the progression of various malignancies. In response to DNA damage, cell survival can be enhanced by activation of DNA repair mechanisms, while simultaneously stimulating energy-expensive cell cycle checkpoints that delay the cell cycle progression to allow more time for DNA repair. This review summarizes our current understanding of the role of clusterin in DNA repair, apoptosis, and ...
Premature senescence of human diploid fibroblasts (HDFs) can be induced by exposures to a variety of oxidative stress and DNA damaging agents. In this study we developed a robust model of UVB-induced premature senescence of skin HDFs. After a series of 10 subcytotoxic (non-proapoptotic) exposures to UVB at 250 mJ/cm2, the so-called biomarkers of senescence were markedly expressed: growth arrest, senescence-associated β-galactosidase activity, senescence-associated gene overexpression, deletion in mitochondrial DNA. A set of 44 stress- and senescence-associated genes were found to be differentially expressed in this model, among which clusterin/apolipoprotein J (apo J) and transforming growth factor-β1 (TGF-β1). Transfection of apo J cDNA provided protection against premature senescence-inducing doses of UVB and other stressful agents. Neutralizing antibodies against TGF-β1 or its receptor II (TβRII) sharply attenuated the senescence-associated features, suggesting a role for TGF-β1 in ...
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TY - JOUR. T1 - Sulfated Glycoproteins, Glycolipids, and Glycosaminoglycans from Synaptic Plasma and Myelin Membranes. T2 - Isolation and Characterization of Sulfated Glycopeptides. AU - Simpson, David L.. AU - Thorne, Donald R.. AU - Loh, Horace H. PY - 1976/12/1. Y1 - 1976/12/1. N2 - In this report we provide biochemical evidence that a highly purified synaptic plasma membrane fraction derived from rat brain, after intraventricular injection of 35S-labeled sodium sulfate, is enriched in a number of large sulfated glycoproteins compared with a purified myelin fraction studied concurrently. A fraction of the detergent-solubilized sulfated glycoprotein bound specifically to concanavalin A-Sepharose. In addition, we have identified the 35S-labeled lipid-soluble material in these membrane fractions as cerebroside sulfate. The sulfated protein in the lipid-extracted membranes was shown to consist predominantly of a class of glycoproteins containing sulfate in ester linkage to oligosaccharide chains, ...
Anti-human Clusterin mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c immunized mice.
Clusterin also named Apolipoprotein J is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid.
Figure 3. Ocular clusterin localization by immunohistochemistry and in situ analysis. Shown are sections of cornea (panels A-C, serial sections), skin/eyelid (panels D, E, serial sections), ciliary body (unfilled arrow and * in panel F), and lens (panels G, H, serial sections). Immunodetection with G7 are shown in panels B, E, F, and H; panels A, D, and G are the negative controls in which the G7 antibody was omitted. In situ hybridization of an anti-sense clusterin mRNA probe on cornea is shown in panel C. Panel I: graph illustrating the distribution of silver grain over different regions of the cornea shown in panel C, for each general region 10 independent counts within a fixed area were taken. Solid arrows indicate the interface between epithelial and stromal cells (cs) in the cornea, arrow heads indicate the area of epithelial cells in the lens.. ...
Loss of cardiomyocytes occurs with aging and contributes to cardiovascular complications. In the present study, we highlighted the role of clusterin, a protein that has recently been associated with the protection of cardiomyocytes from apoptosis. Clusterin protects cardiac cells against damage from myocardial infarction, transplant, or myocarditis. Clusterin can act directly or indirectly on apoptosis by regulating several intracellular pathways. These pathways include (1) the oxidant and inflammatory program, (2) insulin growth factor 1 (IGF-1) pathway, (3) KU70 / BCL-2-associated X protein (BAX) pathway, (4) tumor necrosis factor alpha (TNF-α) pathway, (5) BCL-2 antagonist of cell death (BAD) pathway, and (6) mitogen-activated protein kinase (MAPK) pathway ...
SPECIFIC INFORMATION ABOUT THE FIRST ASYNCHRONOUS WEDNESDAY: DECEMBER 16th. The asynchronous portion of the day will take place in the afternoon or PM portion of the school day for all. The asynchronous focus for this day is digital citizenship. Students will be engaging in a variety of Digital Citizenship See specifics below for each grade. 1st-5th Grade: Students will engage in instruction as they typically would until 12:45pm. At 12:45pm, the asynchronous portion of the day will begin and continue through the end of the school day. Students in grades 1-2 will access the asynchronous work in SeeSaw and students in grades 3-5 will access the asynchronous work in Schoology. Teachers will communicate with students to be sure that they understand the expectations and communicate where they should access the asynchronous work for each of these days. While we prefer that the asynchronous work is completed during this designated time, timing can be adjusted based on what works best for your family as ...
Joel 3:1-21. The Lord Will Be A Refuge Joel 3:1-21 Lesson 3 Key Verse: 3:16b JoelBibleSchool NIU UBF 5/29/08 Delivered by Jennifer Jesmer …But the LORD will be a refuge for his people, a stronghold for the people of Israel. From the last passage I learned that repentance is the most important thing to […]. Read More... ...
This work has been made available to the staff and students of the University of Sydney for the purposes of research and study only. It constitutes material that is held by the University for the purposes of reporting for HERDC and the ERA. This work may not be downloaded, copied and distributed to any third party ...
BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
BioVendor - BioVendor Research and Diagnostic Products is a developer and manufacturer of immunoassays, recombinant proteins, antibodies and endotoxin-removal products.
As we can see the amount of discrete information held in working memory at any one time is quite limited. However, by writing things out as we process our information rather than simply listing the desired outcomes we can formulate a greater number of possibilities.. Lets look through our planners and pick a to-do. Let us suppose we wish to communicate good tidings to Macbeth and hail him Thane of Cawdor. The most obvious choice is to climb to the top of the highest tower and contact him via semaphore. However this would be time consuming not to mention hazardous. What are the options? By exploring the alternatives on paper we have provided ourselves with three choices each one conveys the main message- Macbeth you are now Thane of Cawdor plus a deeper more subjective one. For example:. * Carrier pigeons - Speedy yet impersonal.. * Messenger - slower but makes the receiver feel important.. * Three Fates wearing false beards appear out of thin air - dramatic; the sender is prone to ...
const int SerialWindow::kBaudrates[] = { 50, 75, 110, .... }; SerialWindow::SerialWindow() : .... { .... for(int i = sizeof(kBaudrates) / sizeof(char*); // ,= --i ,= 0;) { message = new BMessage(kMsgSettings); message-,AddInt32(baudrate, kBaudrateConstants[i]); char buffer[7]; sprintf(buffer, %d, kBaudrates[i]); // ,= BMenuItem* item = new BMenuItem(buffer, message); fBaudrateMenu-,AddItem(item ...
While I have always been extremely health conscious and am presently in excellent health, I did become temporarily out-of-commission (i.e. I was really sick) in 2005 with a number of at the time unexplainable symptoms. I was quite puzzled at the time because I had been eating mainly organically grown food, drinking spring water, doing Yoga every morning, and going to the gym several times a week. In other words, I was doing everything one is supposed to do to stay healthy. I was not supposed to get sick. It took me six months before discovering or even imagining the main source of the problem - which was in fact overexposure to electromagnetic - especially microwave - radiation. I was living within 200 meters of two cell phone towers at the time and within 500 meters of a 3rd one with numerous WiFi signals bleeding into my apartment from adjacent neighbors. I developed a host of symptoms, which are found in what has been misleadingly described as Chronic Fatigue Syndrome (CFS) -- but much more ...
Clusterin, ubiquitously distributed in mammalians, was cloned and identified as the most potently induced gene during rat prostate involution following androgen deprivation. Also found to be involved in many other patho-physiological processes, its biological significance is still controversial, particularly with regard to apoptosis. We previously showed that transient over-expression of clusterin blocked cell cycle progression of simian-virus-40-immortalized human prostate epithelial cell lines PNT1A and PNT2. We show in the present study that the accumulation of an intracellular 45 kDa clusterin isoform was an early event closely associated with death of PNT1A cells caused by cell detachment followed by apoptosis induction (anoikis). Cell morphological changes, decreased proliferation rate and cell cycle arrest at G0/G1-S-phase checkpoint were all strictly associated with the production and early translocation to the nucleus of a 45 kDa clusterin isoform. Later, nuclear clusterin was found ...
The CLU gene is located on p21-p12 of human chromosome 8, with CLU as its encoded product, which has various physiological functions, including participating in lipid metabolism (28), oxidative stress reaction (29), and cell cycle regulation (30). CLU is highly expressed in cerebrospinal fluid and amyloid plaques in brain tissues, and is involved in the pathogenesis of AD (4,5,31). Yerbury et al (32) demonstrated that the deposition of CLU in senile plaques and neurofibrillary tangles of AD. Howlett et al (33) further reported a correlation between CLU and senile plaque Aβ40 in the brain cortex of patients with AD. Martin-Rehrmann et al (34) demonstrated the presence of dysfunctional neurons with phosphorylated tau protein surrounding the senile plaques in 71% of CLU-positive patients with AD. Furthermore, they also showed that the tau and phosphorylated tau protein were significantly increased in the rat hippocampus, following the injection of a CLU-rich solution (34). It was suggested that ...
Objective: To investigate the differences between chondrocytes of the superficial and underlying zones of articular cartilage at the level of gene expression. Methods: Messenger RNA (mRNA) was isolated from chondrocytes harvested from the superficial and deep zones of immature bovine articular cartilage. This mRNA was reverse transcribed, radiolabeled, and then each complementary DNA (cDNA) sample was used to screen duplicate filters of a bovine chondrocyte cDNA library. By comparing autoradiographic signals on matching filter sets, clones exclusively expressed in the superficial zone of articular cartilage were isolated and characterized further. Results: Of the superficial-specific gene clones isolated, 25% were found to be a single gene product, clusterin. Northern hybridization was used to show that clusterin is expressed specifically in the superficial zone of articular cartilage and that its expression is up-regulated in mature cartilage. In situ hybridization was used to precisely ...
The blockade of angiotensin II (Ang II) is a significant therapeutic technique for diabetic nephropathy. cultured renal tubular epithelial cell collection, using immunoblot evaluation and real-time RT-PCR. Nuclear localization of NF-B was examined using immunofluorecence and co-immunoprecipitation. Renal fibrosis and manifestation of AT1R was higher in the kidneys of clusterin-/- mice than in those of wild-type mice. Furthermore, lack of clusterin accelerated Ang II-stimulated renal fibrosis and AT1R manifestation. Overexpression of clusterin in proximal tubular epithelial cells reduced the degrees of Ang II-stimulated fibrotic markers and AT1R. Furthermore, intrarenal delivery of clusterin attenuated Ang II-mediated manifestation of fibrotic markers and AT1R in rats. Fluorescence microscopy and co-immunoprecipitation together with traditional western blot exposed that clusterin inhibited Ang II-stimulated nuclear localization of p-NF-B with a immediate physical conversation and subsequently ...
This is a randomized, double-blind, placebo-controlled, multicenter, international trial enrolling patients with metastatic CRPC who had a response to first-line docetaxel therapy and have prostate cancer-related pain with progression of disease. The intended intervention is second-line treatment with docetaxel retreatment or cabazitaxel plus study agent, where custirsen is to be administered in the investigational arm and placebo is to be administered in the control arm.. Selection of the chemotherapy (docetaxel re-treatment or cabazitaxel) is to be determined by the treating physician, based on the patients first-line response.. The study will primarily assess pain and analgesic use for evaluation of durable pain palliation in response to study treatment. Pain and analgesic use will be obtained via a 3rd party contact center (direct contact with patient).. Study treatment starts with a Loading Dose Period during which three infusions of study agent (custirsen vs. placebo) will be ...
Insights into how a gene that increases the risk of Alzheimers disease disrupts brain cells have been revealed by scientists.. Brain tissue from people with Alzheimers showed that a protein called clusterin builds up in vital parts of neurons that connect cells and may damage these links.. Scientists say the findings shed light on the causes of the disease and will help to accelerate the search for a treatment. The study, led by Professor Tara Spires-Jones at the University of Edinburgh, focused on synapses - connections between brain cells that allow the flow of chemical and electrical signals. These signals are vital for forming memories and are key to brain health, experts say.. Researchers showed that synapses in people who had died with Alzheimers contained clumps of clusterin, which could contribute to dementia symptoms. These synapses also contained clumps of amyloid beta, the damaging protein that is found in the brains of people with Alzheimers.. People with a common risk gene, ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Expression of CLU (APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2) in oral mucosa tissue. Antibody staining with HPA000572 and CAB016253 in immunohistochemistry.
α2-Macroglobulin (α2M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. The reaction of α2M with proteases results in an activated conformation, where the proteases become covalently-linked within the interior of a cage-like structure formed by α2M. This study investigates, the effect of activation on the ability of α2M to inhibit amyloid formation by Aβ1-42 and I59T human lysozyme and shows that protease-activated α2M can act via two distinct mechanisms: (i) by trapping proteases that remain able to degrade polypeptide chains and (ii) by a chaperone action that prevents misfolded clients from continuing along the amyloid forming pathway.
Its so hard when you miss someone so much! Sending them this cute and colourful card is the perfect way to tell them theyre missed.. well, at least their face is. - Blank inside for your own personal message- Size: 150mm x 150mm- Recyclable 300gsm card stock- All cards presented in cellophane sleeve- Comes with bro
Interferon-alpha B2 (IFN-a8) (mPEG-ALD), 1 mg. Clusterin also names Apoliprotein J is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have
Mouse monoclonal antibody raised against partial recombinant CLU. Recombinant protein corresponding to a portion of the alpha subunit of human CLU. (MAB9601) - Products - Abnova
With age, forgetfulness and other signs of memory loss sometimes appear, prompting elderly individuals to seek a medical evaluation amid fears that they may be experiencing early symptoms of Alzheimers disease (AD), the ...
The rs4986790 G coding variant in the TLR4 gene appears to reduce AD risk through the modulation of IL-1β synthesis and secretion in the presymptomatic phase of the disease.
April 28,2009- OncoGenex Receives Confirmation from FDA on the Design of a Second Phase 3 Trial Evaluating OGX-011 for the Treatment of Advanced Prostate Cancer.
aha1542: convert to use the data buffer accessors Signed-off-by: Jens Axboe ,[EMAIL PROTECTED], --- drivers/scsi/aha1542.c , 32 +++++++++++++++----------------- 1 files changed, 15 insertions(+), 17 deletions(-) diff --git a/drivers/scsi/aha1542.c b/drivers/scsi/aha1542.c index cbbfbc9..961a188 100644 --- a/drivers/scsi/aha1542.c +++ b/drivers/scsi/aha1542.c @@ -61,15 +61,15 @@ static void BAD_DMA(void *address, unsigned int length) } static void BAD_SG_DMA(Scsi_Cmnd * SCpnt, - struct scatterlist *sgpnt, + struct scatterlist *sgp, int nseg, int badseg) { printk(KERN_CRIT sgpnt[%d:%d] page %p/0x%llx length %u\n, badseg, nseg, - page_address(sgpnt[badseg].page) + sgpnt[badseg].offset, - (unsigned long long)SCSI_SG_PA(&sgpnt[badseg]), - sgpnt[badseg].length); + page_address(sgp-,page) + sgp-,offset, + (unsigned long long)SCSI_SG_PA(sgp), + sgp-,length); /* * Not safe to continue. @@ -691,7 +691,7 @@ static int aha1542_queuecommand(Scsi_Cmnd * SCpnt, void (*done) (Scsi_Cmnd *)) ...
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Results Between ALS patients and healthy controls, there was significant difference in the expression of 30 proteins, including complement proteins and inflammatory markers. Of these, plasma Gelsolin concentration was 1.5 fold higher in healthy control in comparison with ALS patients (p=0.001). There were significant differences in 22 proteins between healthy controls and ALS patients with CI. Clusterin level was 1.2 fold upregulated in heathy people compared with patients with CI (p=0.03). Between ALS with and without CI, there was significant difference in expression of 25 proteins. CD5L concentration was significantly raised in patients without CI (p=0.013). The IPA shows that the complement pathway and coagulation pathway plays an important role in the ALS pathogenesis. ...
The goal of the present study was to determine whether treatment with cigarette smoke extract (CSE) induces cell loss, cellular senescence, and extracellular matrix (ECM) synthesis in primary human retinal pigment epithelial (RPE) cells. Primary cultured human RPE cells were exposed to 2, 4, 8, and 12% of CSE concentration for 24 hours. Cell loss was detected by cell viability assay. Lipid peroxidation was assessed by loss of cis-parinaric acid (PNA) fluorescence. Senescence-associated ß-galactosidase (SA-ß-Gal) activity was detected by histochemical staining. Expression of apolipoprotein J (Apo J), connective tissue growth factor (CTGF), fibronectin, and laminin were examined by real-time PCR, western blot, or ELISA experiments. The results showed that exposure of cells to 12% of CSE concentration induced cell death, while treatment of cells with 2, 4, and 8% CSE increased lipid peroxidation. Exposure to 8% of CSE markedly increased the number of SA-ß-Gal positive cells to up to 82%, and the mRNA
Up to 30% of all hospital admissions and health-care costs may be attributable to alcohol abuse. Ethanol, its oxidative metabolites, acetaldehyde and ROS (reactive oxygen species), non-oxidative metabolites of alcohol [e.g. FAEEs (fatty acid ethyl esters)] and the ethanol-water competition mechanism are all involved in the deregulation of glycoconjugate (glycoprotein, glycolipid and proteoglycan) metabolic processes including biosynthesis, modification, transport, secretion, elimination and catabolism. An increasing number of new alcohol biomarkers that are the result of alcohol-induced glycoconjugate metabolic errors have appeared in the literature. Glycoconjugate-related alcohol markers are involved in, or are a product of, altered glycoconjugate metabolism, e.g. CDT (carbohydrate-deficient transferrin), SA (sialic acid), plasma SIJ (SA index of apolipoprotein J), CETP (cholesteryl ester transfer protein), β-HEX (β-hexosaminidase), dolichol, EtG (ethyl glucuronide) etc. Laboratory tests ...
Sorry if we go quiet for the next week or so - well be out of Office enjoying the festive period! From all of us at DOUK, we hope you have a wonderful Christmas, Hanukkah, Kwanzaa, New Year and any other winter holidays out there! Well be returning to the office on January 2nd, to bring a whole New Years worth of events and exciting new attractions in 2018.. Unfortunately, there will be no updates on DOUK from December 22nd until the date we return. Blogs and news will resume on January 2nd. Have a wonderful festive period! And stay tuned for all the latest events and attractions in 2018.. Keep an eye on our Facebook! Well be posting some stuff on there to hold you all over.. Oh, and for New Years Eve at 11:59:59… HAPPY NEW YEAR! See you all in 2018!. , Back to the news ...
Expression of CLU (APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2) in vagina tissue. Antibody staining with HPA000572 and CAB016253 in immunohistochemistry.
I have an appointment with my favorite vet tomorrow to see what can be done for Tweezer. If he can still be made comfortable by anything I can do, then I will do everything I can for him. If there is nothing that can be done then it will be time to let him go with dignity. I can trust Dr. Tom to advise me correctly. Meanwhile I am a basket case. If Tweezer must crosst the bridge then a bright ray of sunshine will be leaving this world. -Original Message- From: [email protected] [mailto:[email protected]] On Behalf Of Natalie Sent: Thursday, February 03, 2011 3:50 PM To: [email protected] Subject: Re: [Felvtalk] HELP! Tweezer is sick I was giving her sub-q fluids for a while, which I assume helped her because she never had any seizures again. FIV is so much more manageable than FeLV! BTW - I sent the info to the dumb vet at the clinic so she would know for the next time someone brings in a cat that was FIV+, not to immediately make the diagnosis of ...
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Demattos RB, ODell MA, Parsadanian M, Taylor JW, Harmony JA, Bales KR, Paul SM, Aronow BJ, Holtzman DM. Clusterin promotes amyloid plaque formation and is critical for neuritic toxicity in a mouse model of Alzheimers disease ...
Demattos RB, Cirrito JR, Parsadanian M, May PC, ODell MA, Taylor JW, Harmony JA, Aronow BJ, Bales KR, Paul SM, Holtzman DM. ApoE and clusterin cooperatively suppress Abeta levels and deposition: evidence that ApoE regulates extracellular Abeta metabolism in vivo ...
A phase III ITT controlled trial of Cbz/P/C in pts with previously-treated metastatic, castration-resistant PC shown no significant survival gain
Progression Free Survival: time from date of randomization to first objective documented progression per Response Evaluation Criteria In Solid Tumors (RECIST) v1.1 or death due to any cause, whichever occurs first. Tumor lesions measured in at least one dimension with minimum size of 10 mm by CT scan, 10 mm caliper by clinical exam. Malignant lymph nodes must be ,15 mm in short axis when assessed by CT scan. All measurable lesions up to a maximum of 2 lesions per organ and 5 in total representative of all involved organs should be identified as target lesions and measured and recorded ...
A study carried out with a new human stem cell-derived model reveals that the most prevalent genetic risk factor of Alzheimers disease (AD), apolipoprotein E4 (APOE4), impairs the function of human brain immune cells, microglia.
Background: Bridging integrator 1 (BIN1 ) has been identified as one of the most associated loci for Alzheimers disease (AD), and recently was reported to modulate tau pathology to mediate AD in vitro . However, the effects of BIN1 on the AD related
Protein expressions of Prx II and CLU in plasma by Western blot analysis. A: Single samples of normal and liver fibrosis. Immunoblotting with Prx II or CLU poly
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"Entrez Gene: clusterin". Koltai T (2014). "Clusterin: a key player in cancer chemoresistance and its inhibition". OncoTargets ... In humans, clusterin is encoded by the CLU gene on chromosome 8. CLU is a molecular chaperone responsible for aiding protein ... Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and ... Wei L, Xue T, Wang J, Chen B, Lei Y, Huang Y, Wang H, Xin X (Aug 2009). "Roles of clusterin in progression, chemoresistance and ...
Clusterin associated protein 1, also known as CLUAP1, is a human gene. Model organisms have been used in the study of CLUAP1 ... "Entrez Gene: clusterin associated protein 1". Retrieved 2011-08-30. "Radiography data for Cluap1". Wellcome Trust Sanger ...
... acts as an anti-cancer drug by binding to the mRNA initiation site of the clusterin gene, reducing clusterin protein ... The results found that the recommended dose of Custirsen was 640 mg, with maximum decrease of clusterin blood plasma occurring ... The anti-apoptotic activity of clusterin in aiding tumour growth is due to interactions with protein complexes. These include: ... Chi KN, Zoubeidi A, Gleave ME (December 2008). "Custirsen (OGX-011): a second-generation antisense inhibitor of clusterin for ...
Zhou Y, Li L, Hu L, Peng T (March 2011). "Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1) interacts with secretory clusterin". ...
Bajari TM, Strasser V, Nimpf J, Schneider WJ (2003). "A model for modulation of leptin activity by association with clusterin ...
Clusterin - Clusterin is a protein ubiquitously expressed in different cell lines. Secreted clusterin is involved in lipid ... The cells release clusterin as a response to cell stress. This is due to clusterin being able to protect the cell by reducing ... Kidney ischemia can be diagnosed by checking the levels of several biomarkers such as clusterin and cystatin C. While the ...
Clusterin is a heterodimeric protein that aids in the clearance of cellular debris and is involved with apoptosis. Clusterin ... Grogg, Karen L; Macon, William R; Kurtin, Paul J; Nascimento, Antonio G (2004). "A survey of clusterin and fascin expression in ... These cells are best visualized with immunostaining using the FDC markers CD21, CD35, R4/23, clusterin, and KiM4p. Marker ... Characteristically, FDCS have mircotubuloreticular structures (MTRS) and increased levels of intracellular clusterin. MTRS ...
"Identification of glycoprotein 330 as an endocytic receptor for apolipoprotein J/clusterin". J. Biol. Chem. 270 (22): 13070-5. ...
Vandepoele K, Staes K, Andries V, van Roy F (April 2010). "Chibby interacts with NBPF1 and clusterin, two candidate tumor ...
"Identification of human plasma proteins as major clients for the extracellular chaperone clusterin". The Journal of Biological ...
Antiapoptotic proteins, such as clusterin and survivin, are often expressed in cancer cells. Clusterin and survivin-targeting ... "Nucleotide-based therapies targeting clusterin chemosensitize human lung adenocarcinoma cells both in vitro and in vivo". ...
It appears together with clusterin and complement C3, markers of complement-mediated inflammatory reactions. Also Fibroblast ...
Lee KB, Jeon JH, Choi I, Kwon OY, Yu K, You KH (February 2008). "Clusterin, a novel modulator of TGF-beta signaling, is ...
Plasma clusterin and the risk of Alzheimer disease. JAMA. 2011 Apr 6;305(13):1322-6. Solouki AM, et al. A genome-wide ...
9 (3). Proteome Science , Full text , Development of reverse phase protein microarrays for the validation of clusterin, a mid- ...
Gleave's team was recognized in 2010 as the first in the world to develop an anti-clusterin agent. Richard D. Williams MD ...
2003). "Clusterin, an abundant serum factor, is a possible negative regulator of MT6-MMP/MMP-25 produced by neutrophils". J. ...
In these cases, the FD cells express FD-cell markers (e.g. CD21, CD23, CD35, clusterin, podoplanin, gamma-synuclein) and in >90 ...
These fluid phase complexes do not bind to cell membranes and are ultimately scavenged by clusterin and vitronectin, two ...
Clusterin, a soluble molecule with functions similar to CD59, forms a complex with Granzyme B and inhibits activation of ...
... is accompanied by significant changes in the levels of expression of polyamine metabolism regulatory genes and clusterin ( ...
Li, W.; Piecuch, P. (2010). "Multi-level Extension of the Cluster-In-Molecule Local Correlation Methodology: Merging Coupled- ...
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... clusterin, and osteopontin (OPN), and in humans, insulin-like growth factor binding protein 7 (IGFBP7) as part of the ...
... the gene for clusterin CLU (programming language) Clu (Tron), fictional character from the Tron franchise Chartered Life ...
The following list gives an overview of the problems supported by HeuristicLab: Artificial Ant Classification Clusterin ...
... turnkey cluster-in-a-box solution that can be purchased, leased, or rented by smaller companies or individuals who want to ...
Among soluble inhibitors there are factor H, C1 inhibitor, C4b-binding protein, factor I, S protein or clusterin, the membrane- ...
For example, expression of CD56, MUC1 (also termed EMA for epithelial membrane antigen), and clusterin and strong uniform ...
Clusterin - Monoclonal Antibody - from Mouse - for Immunohistochemistry - Antibodies. Product filter Clusterin molecule ... Clusterin Connective Tissue Growth Factor CXCL12 Cystatin C Decorin Endostatin ENPP1 Epidermal Fatty Acid Binding Protein ERK1 ...
Upon interaction with Tau aggregates, Clusterin stabilizes highly potent, soluble seed species. Tau/Clusterin complexes enter ... The extracellular chaperone Clusterin enhances Tau aggregate seeding in a cellular model. View ORCID ProfilePatricia Yuste- ... Thus, upregulation of Clusterin, as observed in AD patients, may enhance Tau seeding and possibly accelerate the spreading of ... Here we show in a cellular model system and in neurons that Clusterin, an abundant extracellular chaperone, strongly enhances ...
... all of which interact with clusterin to form long-lived, stable complexes. Consequently, clusterin is able to influence both ... These results not only elucidate the protective role of clusterin but also provide a molecular basis for the genetic link ... We have examined the interactions between human clusterin and the Alzheimers disease-associated amyloid-β 1-40 peptide (Aβ 1- ... clusterin, has been identified as a newly-discovered risk factor in Alzheimers disease. ...
Human - Clusterin - Monoclonal Antibody - from Mouse - for Immunohistochemistry - Antibodies. Product filter Clusterin molecule ... Clusterin Connective Tissue Growth Factor CXCL12 Cystatin C Decorin Endostatin ENPP1 Epidermal Fatty Acid Binding Protein ERK1 ...
Clusterin. Clusterin, a plasma protein, plays an important role in the pathogenesis of AD. In one study, clusterin was ... Plasma clusterin and the risk of Alzheimer disease. JAMA. 2011 Apr 6. 305(13):1322-6. [QxMD MEDLINE Link]. ... Association of plasma clusterin concentration with severity, pathology, and progression in Alzheimer disease. Arch Gen ... 19] A study by Schrijvers et al notes that although plasma clusterin levels are significantly associated with baseline ...
ApoJ/clusterin is a multifunctional protein highly expressed in the brain. The implication of ApoJ in β-amyloid (Aβ) ... Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice. Alzforum ... Peripheral administration of human recombinant ApoJ/clusterin modulates brain beta-amyloid levels in APP23 mice. Access & ...
The role of clusterin in tumor growth and progression remains unclear. Overexpression of cytoplasmic clusterin has been studied ... Clusterin/*metabolism. Colorectal Neoplasms/*metabolism/pathology. Female. Humans. Immunoenzyme Techniques. In Situ Nick-End ... We assessed levels of clusterin expression in a group of stage II colorectal cancer patients to assess its utility as a ... High clusterin expression correlates with a poor outcome in stage II colorectal cancers.. ...
The ability of clusterin to influence amyloid fibril formation prompted us to investigate whether clusterin is capable of ... The ability of clusterin to influence amyloid fibril formation prompted us to investigate whether clusterin is capable of ... The ability of clusterin to influence amyloid fibril formation prompted us to investigate whether clusterin is capable of ... The ability of clusterin to influence amyloid fibril formation prompted us to investigate whether clusterin is capable of ...
This panel allows simultaneous quantification of 9 human proteins, including IGFBP7, B2M, Cystatin C, Clusterin, EGF, Albumin, ...
The clusterin assay is a solid phase ELISA designed to measure humin clusterin in cell culture supernates, serum, plasma, urine ... Mature human clusterin shares a 77% amino acid sequence identity with mouse and rat clusterin. ... Determination of urine clusterin has been shown to have utility in tracking kidney injury in the animals. Thus, it detects ... Clusterin (also know as apolipoprotein J, sulfated glycoprotein 2 (SGP-2), TRPM-2, and SP-40) is a secreted multifunctional ...
Relationship between Clusterin Gene Expression and In-vitro Sperm Characteristics in Caprine Semen. Journal of Animal Research ... Relationship between Clusterin Gene Expression and In-vitro Sperm Characteristics in Caprine Semen. ... The present experiment was undertaken to study the relationship between clusterin (CLU) gene expression and in vitro sperm ...
Solved: I am running a multisite cluster on Splunk 6.2.5. Is that why? (does the GUI not support managing a multisite cluster yet)?
Mature mouse Clusterin shares 77% and 93% amino acid sequence identity with human and rat Clusterin, respectively.? ... Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40, is a secreted multifunctional ... It also participates in the control of cell proliferation, apoptosis, and carcinogenesis (1, 2). Clusterin is expressed in ... Mouse Clusterin is synthesized as a precursor that contains two coiled coil domains, two nuclear localization signals (NLS), ...
Clusterin (CLU) is an extracellular chaperone that is likely to play an important role in protein folding quality control. This ... Clusterin (CLU) is an extracellular chaperone that is likely to play an important role in protein folding quality control. This ... Identification of human plasma proteins as major clients for the extracellular chaperone clusterin Journal Article Download ... Identification of human plasma proteins as major clients for the extracellular chaperone clusterin. Journal of Biological ...
Clusterin+Albumin+Osteopontin+Cystatin C+TIM 1+Lipocalin-2 / NGAL (2). * Clusterin+Osteopontin+Cystatin C+TIM 1+Lipocalin-2 / ...
Golden Frog added a new VyprVPN server cluster in Miami! Feedback from Golden Frog Ideas motivated the addition of this new server location, and Golden Frog encourages Giganews members using VyprVPN to share or vote up ideas. Your feedback is extremely useful to the Golden Frog team and they take all ideas seriously.. The new server cluster is available for all VyprVPN protocols, including PPTP, L2TP/IPsec, and OpenVPN.. How to Access the New Miami VyprVPN Server Cluster:. ...
Clusterin (CLU) is an extracellular chaperone involved in reducing amyloid beta (Aβ) toxicity and aggregation. Although ... Clusterin (CLU) is an extracellular chaperone involved in reducing amyloid beta (Aβ) toxicity and aggregation. Although ... Genetic variant rs11136000 upregulates clusterin expression and reduces Alzheimers disease risk. by admin ...
Clusterin. A highly conserved heterodimeric glycoprotein that is differentially expressed during many severe physiological ... Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE. ...
Wu C-Y, Yang H-Y, Chien H-P, Tseng M-H, Huang J-L (2018) Urinary clusterin-a novel urinary biomarker associated with pediatric ... Hidaka S, Kranzlin B, Gretz N, Witzgall R (2002) Urinary clusterin levels in the rat correlate with the severity of tubular ... Dieterle F, Perentes E, Cordier A et al (2010a) Urinary clusterin, cystatin C, beta2-microglobulin and total protein as markers ... Another early marker of renal damage is urinary clusterin (CLU), which is a heterodimeric glycoprotein that contributes to ...
You have now acquired the basic knowledge to properly create and configure a cluster using Oracle Public Cloud and MySQL Cloud Service instances, including how to bootstrap a remote cluster so that you can access it locally. This scenario is useful when you dont want to expose the servers where MySQL Server is running but instead provide the IP address of another server which just handles the application traffic.. Theres a lot of other functionalities that we still want to share with you, so stay tuned for Part II of this series!. If you have any questions about the basic setup described here, please let us know here in the comments. As always, thank you for using MySQL!. ...
ANALISIS PERSEBARAN KASUS COVID-19 DI JAWA BARAT MENGGUNAKAN METODE K-MEANS CLUSTERIN Authors. * Elsa Ramadanti Universitas ...
Gene: [08p21/CLU] clusterin (complement lysis inhibitor, SP-40,40, apolipoprotein J); sulfated glycoprotein 2 (testosterone- ...
Clusterin transduces Alzheimer-risk signals to amyloidogenesis. Liu X, Che R, Liang W, Zhang Y, Wu L, Han C, Lu H, Song W, Wu Y ...
i-k, Quantification of relative spectral counts for i, Clusterin (CLU); j, Apolipoprotein E (APOE) and k, Amyloid Precursor ...
Also searched for Cerenkov Luminescence Imaging and Clusterin. See Search Details. *List ...
clusterin. ISO. RGD. PMID:10502582. RGD:8699502. NCBI chr15:40,161,068...40,200,315 Ensembl chr15:40,174,617...40,200,315 ... clusterin. ISO. RGD. PMID:18378577 PMID:22956607. RGD:8696020, RGD:8661808. NCBI chr15:40,161,068...40,200,315 Ensembl chr15: ...
clusterin. CLU. 282. CNR1. cannabinoid receptor 1 (brain). CNR1. 132. COQ7. coenzyme Q7 homolog, ubiquinone (yeast). COQ7. ...
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  • The extracellular chaperone clusterin sequesters oligomeric forms of t" by Priyanka Narayan, Angel Orte et al. (edu.au)
  • The ability of clusterin to influence amyloid fibril formation prompted us to investigate whether clusterin is capable of inhibiting TTR amyloidosis. (elsevier.com)
  • The ability of clusterin to bind and neutralize non-oxidatively modified LDL reduces cytotoxicity in atherosclerotic plaques. (nexelis.com)
  • We also demonstrated that clusterin is an effective inhibitor of L55P TTR amyloidosis, the most aggressive form of TTR diseases. (elsevier.com)
  • Clusterin in Alzheimer's disease: An amyloidogenic inhibitor of amyloid formation? (grnet.gr)
  • In recent genome-wide association studies, the extracellular chaperone protein, clusterin, has been identified as a newly-discovered risk factor in Alzheimer's disease. (edu.au)
  • We have examined the interactions between human clusterin and the Alzheimer's disease-associated amyloid-β 1-40 peptide (Aβ 1-40 ), which is prone to aggregate into an ensemble of oligomeric intermediates implicated in both the proliferation of amyloid fibrils and in neuronal toxicity. (edu.au)
  • These results not only elucidate the protective role of clusterin but also provide a molecular basis for the genetic link between clusterin and Alzheimer's disease. (edu.au)
  • The chaperone function of clusterin helps to reduce the accumulation of the β-amyloid fibrils and damage due to amyloid plaques in Alzheimer's disease. (nexelis.com)
  • The role of clusterin in tumor growth and progression remains unclear. (lenus.ie)
  • Increased circulating levels of clusterin enhance tumor aggressiveness by inhibiting apoptosis and by promoting the epithelial to messenchymal transition. (nexelis.com)
  • Evaluation of genetic polymorphisms in clusterin and tumor necrosis factor-alpha genes in South Indian individuals with pseudoexfoliation syndrome. (cdc.gov)
  • Mature human clusterin shares a 77% amino acid sequence identity with mouse and rat clusterin. (nexelis.com)
  • Clusterin (also know as apolipoprotein J, sulfated glycoprotein 2 (SGP-2), TRPM-2, and SP-40) is a secreted multifunctional protein that was named for its ability to induce cellular clustering. (nexelis.com)
  • Clusterin (CLU) is an extracellular chaperone that is likely to play an important role in protein folding quality control. (edu.au)
  • Custirsen utilizes second-generation antisense technology, licensed from Isis Pharmaceuticals (NASDAQ: ISIS), to target and inhibit production of clusterin, a protein involved in resistance of cancer tumors to treatments. (higcapital.com)
  • Tau/Clusterin complexes enter recipient cells via endocytosis and compromise the endolysosomal compartment, allowing transfer to the cytosol where they propagate aggregation of endogenous Tau. (biorxiv.org)
  • Using highly sensitive single-molecule fluorescence methods, we have found that Aβ 1-40 forms a heterogeneous distribution of small oligomers (from dimers to 50-mers), all of which interact with clusterin to form long-lived, stable complexes. (edu.au)
  • The present experiment was undertaken to study the relationship between clusterin (CLU) gene expression and in vitro sperm characteristics in buck semen. (who.int)
  • Genetic analysis of the clusterin gene in pseudoexfoliation syndrome. (cdc.gov)
  • Here we show in a cellular model system and in neurons that Clusterin, an abundant extracellular chaperone, strongly enhances Tau aggregate seeding. (biorxiv.org)
  • Clusterin has recently been proposed to play a role as an extracellular molecular chaperone, affecting the fibril formation of amyloidogenic proteins. (elsevier.com)
  • Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE. (nih.gov)
  • 2010). Association of plasma clusterin concentration with severity, pathology, and progression in Alzheimer disease. (bvsalud.org)
  • Influence of clusterin genetic variants on IOP elevation in pseudoexfoliation syndrome and pseudoexfoliative glaucoma in Turkish population. (cdc.gov)
  • Here, we report that clusterin strongly interacts with wild-type TTR and TTR variants V30M and L55P under acidic conditions, and blocks the amyloid fibril formation of TTR variants. (elsevier.com)
  • In particular, the amyloid fibril formation of V30M TTR in the presence of clusterin is reduced to level similar to wild-type TTR. (elsevier.com)
  • The clusterin assay is a solid phase ELISA designed to measure humin clusterin in cell culture supernates, serum, plasma, urine and saliva. (nexelis.com)
  • Glicoproteína heterodimérica, muy estable, que se expresa en muy diversos trastornos fisiológicos graves, como el CÁNCER, la APOPTOSIS y algunas TRASTORNOS NEUROLÓGICOS. (bvsalud.org)
  • Association of clusterin (CLU) variants and exfoliation syndrome: An analysis in two Caucasian studies and a meta-analysis. (cdc.gov)
  • Thus, upregulation of Clusterin, as observed in AD patients, may enhance Tau seeding and possibly accelerate the spreading of Tau pathology. (biorxiv.org)
  • Determination of urine clusterin has been shown to have utility in tracking kidney injury in the animals. (nexelis.com)
  • Consequently, clusterin is able to influence both the aggregation and disaggregation of Aβ 1-40 by sequestration of the Aβ oligomers. (edu.au)
  • Human clusterin is synthesized as a precursor that contains two coiled domains, three nuclear localization signals (NLS), and one heparin binding domain. (nexelis.com)
  • An alternately spliced 50 kDa isoform o f human clusterin remains intracellularly and is neither glycosylated nor cleaved into α and β chains. (nexelis.com)
  • Prevention of Dominant IgG Adsorption on Nanocarriers in IgG-Enriched Blood Plasma by Clusterin Precoating. (mpg.de)
  • We assessed levels of clusterin expression in a group of stage II colorectal cancer patients to assess its utility as a prognostic marker. (lenus.ie)
  • Clusterin levels are associated with poor survival in stage II colorectal cancer. (lenus.ie)
  • High levels (µg/mL) of clusterin circulate predominantly as a component of high-density lipoprotein particles, and these are internalized and degraded through interactions with LPR-2/megalin. (nexelis.com)
  • however, no correlation between clusterin expression and survival in colorectal cancer has been identified to date. (lenus.ie)
  • High clusterin expression correlates with a poor outcome in stage II colorectal cancers. (lenus.ie)
  • The mechanism by which clusterin inhibits TTR amyloidosis appears to be through stabilization of TTR tetrameric structure. (elsevier.com)
  • This function contrasts with the cyoprotective effect of secreted clusterin. (nexelis.com)
  • In addition, clusterin (two out of six isoforms) and haptoglobin (four isoforms) were up-regulated in bGH mice as a function of age. (nih.gov)