Clusterin: A highly conserved heterodimeric glycoprotein that is differentially expressed during many severe physiological disturbance states such as CANCER; APOPTOSIS; and various NEUROLOGICAL DISORDERS. Clusterin is ubiquitously expressed and appears to function as a secreted MOLECULAR CHAPERONE.Molecular Chaperones: A family of cellular proteins that mediate the correct assembly or disassembly of polypeptides and their associated ligands. Although they take part in the assembly process, molecular chaperones are not components of the final structures.Glycoproteins: Conjugated protein-carbohydrate compounds including mucins, mucoid, and amyloid glycoproteins.Complement Inactivator Proteins: Serum proteins that negatively regulate the cascade process of COMPLEMENT ACTIVATION. Uncontrolled complement activation and resulting cell lysis is potentially dangerous for the host. The complement system is tightly regulated by inactivators that accelerate the decay of intermediates and certain cell surface receptors.Rete Testis: The network of channels formed at the termination of the straight SEMINIFEROUS TUBULES in the mediastinum testis. Rete testis channels drain into the efferent ductules that pass into the caput EPIDIDYMIS.Apolipoproteins E: A class of protein components which can be found in several lipoproteins including HIGH-DENSITY LIPOPROTEINS; VERY-LOW-DENSITY LIPOPROTEINS; and CHYLOMICRONS. Synthesized in most organs, Apo E is important in the global transport of lipids and cholesterol throughout the body. Apo E is also a ligand for LDL receptors (RECEPTORS, LDL) that mediates the binding, internalization, and catabolism of lipoprotein particles in cells. There are several allelic isoforms (such as E2, E3, and E4). Deficiency or defects in Apo E are causes of HYPERLIPOPROTEINEMIA TYPE III.Sialic Acids: A group of naturally occurring N-and O-acyl derivatives of the deoxyamino sugar neuraminic acid. They are ubiquitously distributed in many tissues.Carbohydrates: The largest class of organic compounds, including STARCH; GLYCOGEN; CELLULOSE; POLYSACCHARIDES; and simple MONOSACCHARIDES. Carbohydrates are composed of carbon, hydrogen, and oxygen in a ratio of Cn(H2O)n.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Mammaglobin B: A MAMMAGLOBIN A-related secretoglobin that is expressed in several HUMAN tissues including the UTERUS; BREAST; SALIVARY GLAND; and LACRIMAL GLAND.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Alzheimer Disease: A degenerative disease of the BRAIN characterized by the insidious onset of DEMENTIA. Impairment of MEMORY, judgment, attention span, and problem solving skills are followed by severe APRAXIAS and a global loss of cognitive abilities. The condition primarily occurs after age 60, and is marked pathologically by severe cortical atrophy and the triad of SENILE PLAQUES; NEUROFIBRILLARY TANGLES; and NEUROPIL THREADS. (From Adams et al., Principles of Neurology, 6th ed, pp1049-57)BooksPublishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.MEDLINE: The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).SwedenSoftware: Sequential operating programs and data which instruct the functioning of a digital computer.User-Computer Interface: The portion of an interactive computer program that issues messages to and receives commands from a user.Internet: A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.Word Processing: Text editing and storage functions using computer software.Computer Graphics: The process of pictorial communication, between human and computers, in which the computer input and output have the form of charts, drawings, or other appropriate pictorial representation.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Toremifene: A first generation selective estrogen receptor modulator (SERM). Like TAMOXIFEN, it is an estrogen agonist for bone tissue and cholesterol metabolism but is antagonistic on mammary and uterine tissue.Estrogen Receptor Modulators: Substances that possess antiestrogenic actions but can also produce estrogenic effects as well. They act as complete or partial agonist or as antagonist. They can be either steroidal or nonsteroidal in structure.Tamoxifen: One of the SELECTIVE ESTROGEN RECEPTOR MODULATORS with tissue-specific activities. Tamoxifen acts as an anti-estrogen (inhibiting agent) in the mammary tissue, but as an estrogen (stimulating agent) in cholesterol metabolism, bone density, and cell proliferation in the ENDOMETRIUM.Estrogen Antagonists: Compounds which inhibit or antagonize the action or biosynthesis of estrogenic compounds.Breast Neoplasms: Tumors or cancer of the human BREAST.Neoadjuvant Therapy: Preliminary cancer therapy (chemotherapy, radiation therapy, hormone/endocrine therapy, immunotherapy, hyperthermia, etc.) that precedes a necessary second modality of treatment.Stem Cell Research: Experimentation on STEM CELLS and on the use of stem cells.Apoptosis: One of the mechanisms by which CELL DEATH occurs (compare with NECROSIS and AUTOPHAGOCYTOSIS). Apoptosis is the mechanism responsible for the physiological deletion of cells and appears to be intrinsically programmed. It is characterized by distinctive morphologic changes in the nucleus and cytoplasm, chromatin cleavage at regularly spaced sites, and the endonucleolytic cleavage of genomic DNA; (DNA FRAGMENTATION); at internucleosomal sites. This mode of cell death serves as a balance to mitosis in regulating the size of animal tissues and in mediating pathologic processes associated with tumor growth.Inclusion Bodies: A generic term for any circumscribed mass of foreign (e.g., lead or viruses) or metabolically inactive materials (e.g., ceroid or MALLORY BODIES), within the cytoplasm or nucleus of a cell. Inclusion bodies are in cells infected with certain filtrable viruses, observed especially in nerve, epithelial, or endothelial cells. (Stedman, 25th ed)Vimentin: An intermediate filament protein found in most differentiating cells, in cells grown in tissue culture, and in certain fully differentiated cells. Its insolubility suggests that it serves a structural function in the cytoplasm. MW 52,000.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Eye: The organ of sight constituting a pair of globular organs made up of a three-layered roughly spherical structure specialized for receiving and responding to light.Sclera: The white, opaque, fibrous, outer tunic of the eyeball, covering it entirely excepting the segment covered anteriorly by the cornea. It is essentially avascular but contains apertures for vessels, lymphatics, and nerves. It receives the tendons of insertion of the extraocular muscles and at the corneoscleral junction contains the canal of Schlemm. (From Cline et al., Dictionary of Visual Science, 4th ed)PrimatesEye Movements: Voluntary or reflex-controlled movements of the eye.Gelsolin: A 90-kDa protein produced by macrophages that severs ACTIN filaments and forms a cap on the newly exposed filament end. Gelsolin is activated by CALCIUM ions and participates in the assembly and disassembly of actin, thereby increasing the motility of some CELLS.Amyotrophic Lateral Sclerosis: A degenerative disorder affecting upper MOTOR NEURONS in the brain and lower motor neurons in the brain stem and SPINAL CORD. Disease onset is usually after the age of 50 and the process is usually fatal within 3 to 6 years. Clinical manifestations include progressive weakness, atrophy, FASCICULATION, hyperreflexia, DYSARTHRIA, dysphagia, and eventual paralysis of respiratory function. Pathologic features include the replacement of motor neurons with fibrous ASTROCYTES and atrophy of anterior SPINAL NERVE ROOTS and corticospinal tracts. (From Adams et al., Principles of Neurology, 6th ed, pp1089-94)CD40 Ligand: A membrane glycoprotein and differentiation antigen expressed on the surface of T-cells that binds to CD40 ANTIGENS on B-LYMPHOCYTES and induces their proliferation. Mutation of the gene for CD40 ligand is a cause of HYPER-IGM IMMUNODEFICIENCY SYNDROME, TYPE 1.Superoxide Dismutase: An oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against dangerous levels of superoxide. EC 1.15.1.1.Motor Neurons: Neurons which activate MUSCLE CELLS.Biological Markers: Measurable and quantifiable biological parameters (e.g., specific enzyme concentration, specific hormone concentration, specific gene phenotype distribution in a population, presence of biological substances) which serve as indices for health- and physiology-related assessments, such as disease risk, psychiatric disorders, environmental exposure and its effects, disease diagnosis, metabolic processes, substance abuse, pregnancy, cell line development, epidemiologic studies, etc.tau Proteins: Microtubule-associated proteins that are mainly expressed in neurons. Tau proteins constitute several isoforms and play an important role in the assembly of tubulin monomers into microtubules and in maintaining the cytoskeleton and axonal transport. Aggregation of specific sets of tau proteins in filamentous inclusions is the common feature of intraneuronal and glial fibrillar lesions (NEUROFIBRILLARY TANGLES; NEUROPIL THREADS) in numerous neurodegenerative disorders (ALZHEIMER DISEASE; TAUOPATHIES).Age of Onset: The age, developmental stage, or period of life at which a disease or the initial symptoms or manifestations of a disease appear in an individual.Neurofibrillary Tangles: Abnormal structures located in various parts of the brain and composed of dense arrays of paired helical filaments (neurofilaments and microtubules). These double helical stacks of transverse subunits are twisted into left-handed ribbon-like filaments that likely incorporate the following proteins: (1) the intermediate filaments: medium- and high-molecular-weight neurofilaments; (2) the microtubule-associated proteins map-2 and tau; (3) actin; and (4) UBIQUITINS. As one of the hallmarks of ALZHEIMER DISEASE, the neurofibrillary tangles eventually occupy the whole of the cytoplasm in certain classes of cell in the neocortex, hippocampus, brain stem, and diencephalon. The number of these tangles, as seen in post mortem histology, correlates with the degree of dementia during life. Some studies suggest that tangle antigens leak into the systemic circulation both in the course of normal aging and in cases of Alzheimer disease.National Institute on Aging (U.S.): Component of the NATIONAL INSTITUTES OF HEALTH. Through basic and clinical biomedical research and training, it conducts and supports research into the nature of the aging process and diseases associated with the later stages of life. The Institute was established in 1974.Lipocalins: A diverse family of extracellular proteins that bind to small hydrophobic molecules. They were originally characterized as transport proteins, however they may have additional roles such as taking part in the formation of macromolecular complexes with other proteins and binding to CELL SURFACE RECEPTORS.Archaea: One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.Acute Kidney Injury: Abrupt reduction in kidney function. Acute kidney injury encompasses the entire spectrum of the syndrome including acute kidney failure; ACUTE KIDNEY TUBULAR NECROSIS; and other less severe conditions.Receptors, Virus: Specific molecular components of the cell capable of recognizing and interacting with a virus, and which, after binding it, are capable of generating some signal that initiates the chain of events leading to the biological response.Kidney: Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.

Recovery following relief of unilateral ureteral obstruction in the neonatal rat. (1/535)

BACKGROUND: Obstructive nephropathy is a primary cause of renal insufficiency in infants and children. This study was designed to distinguish the reversible and irreversible cellular consequences of temporary unilateral ureteral obstruction (UUO) on the developing kidney. METHODS: Rats were subjected to UUO or sham operation in the first 48 hours of life, and the obstruction was removed five days later (or was left in place). Kidneys were removed for study 14 or 28 days later. In additional groups, kidneys were removed at the end of five days of obstruction. Immunoreactive distribution of renin was determined in arterioles, and the distribution of epidermal growth factor, transforming growth factor-beta1, clusterin, vimentin, and alpha-smooth muscle actin was determined in tubules and/or interstitium. The number of glomeruli, glomerular maturation, tubular atrophy, and interstitial collagen deposition was determined by morphometry. Renal cellular proliferation and apoptosis were measured by proliferating cell nuclear antigen and the TdT uridine-nick-end-label technique, respectively. The glomerular filtration rate was measured by inulin clearance. RESULTS: Renal microvascular renin maintained a fetal distribution with persistent UUO; this was partially reversed by the relief of obstruction. Although glomerular maturation was also delayed and glomerular volume was reduced by UUO, the relief of obstruction prevented the reduction in glomerular volume. Although relief of obstruction did not reverse a 40% reduction in the number of nephrons, the glomerular filtration rate of the postobstructed kidney was normal. The relief of obstruction did not improve tubular cell proliferation and only partially reduced apoptosis induced by UUO. This was associated with a persistent reduction in the tubular epidermal growth factor. In addition, the relief of obstruction reduced but did not normalize tubular expression of transforming growth factor-beta1, clusterin, and vimentin, all of which are evidence of persistent tubular injury. The relief of obstruction significantly reduced interstitial fibrosis and expression of alpha-smooth muscle actin by interstitial fibroblasts, but not to normal levels. CONCLUSIONS: The relief of obstruction in the neonatal rat attenuates, but does not reverse, renal vascular, glomerular, tubular, and interstitial injury resulting from five days of UUO. Hyperfiltration by remaining nephrons and residual tubulointerstitial injury in the postobstructed kidney are likely to lead to deterioration of renal function later in life.  (+info)

Clusterin has chaperone-like activity similar to that of small heat shock proteins. (2/535)

Clusterin is a highly conserved protein which is expressed at increased levels by many cell types in response to a broad variety of stress conditions. A genuine physiological function for clusterin has not yet been established. The results presented here demonstrate for the first time that clusterin has chaperone-like activity. At physiological concentrations, clusterin potently protected glutathione S-transferase and catalase from heat-induced precipitation and alpha-lactalbumin and bovine serum albumin from precipitation induced by reduction with dithiothreitol. Enzyme-linked immunosorbent assay data showed that clusterin bound preferentially to heat-stressed glutathione S-transferase and to dithiothreitol-treated bovine serum albumin and alpha-lactalbumin. Size exclusion chromatography and SDS-polyacrylamide gel electrophoresis analyses showed that clusterin formed high molecular weight complexes (HMW) with all four proteins tested. Small heat shock proteins (sHSP) also act in this way to prevent protein precipitation and protect cells from heat and other stresses. The stoichiometric subunit molar ratios of clusterin:stressed protein during formation of HMW complexes (which for the four proteins tested ranged from 1.0:1.3 to 1.0:11) is less than the reported ratios for sHSP-mediated formation of HMW complexes (1.0:1.0 or greater), indicating that clusterin is a very efficient chaperone. Our results suggest that clusterin may play a sHSP-like role in cytoprotection.  (+info)

Clusterin gene expression mediates resistance to apoptotic cell death induced by heat shock and oxidative stress. (3/535)

Clusterin is a widely expressed, well conserved, secreted glycoprotein, which is highly induced in tissues regressing as a consequence of apoptotic cell death in vivo. It has recently been shown that clusterin expression is only confined to surviving cells following the induction of apoptosis in vitro, suggesting that it is involved in cell survival rather than death. In the hypothesis that clusterin may be implicated in cellular responses to stress, clusterin gene expression was analyzed in the A431 human epidermoid cancer cell line following heat shock and oxidative stress. Our results show that both a transient heat shock (20 min at 42 degrees C) and various oxidative stresses, including hydrogen peroxide, superoxide anion, hyperoxia and UVA exposure, induce a strong increase in clusterin mRNA levels as assessed by northern blot. Nuclear run-on analysis suggests that transcriptional activation is involved in inducing clusterin mRNA in response to heat shock. Using pulse-chase analysis of control and heat shocked cells, it is shown that clusterin mRNA is translated and secreted, thus resulting in increased extracellular levels of the protein following heat shock. To investigate the function of clusterin in response to these stresses, clusterin anti-sense transfectants that stably express virtually no clusterin at the mRNA and protein level were generated in A431 cells. These anti-sense transfectants are shown to be highly sensitive to apoptotic cell death induced by heat shock or oxidative stress compared with wild-type A431 cells or control transfectants. Taken together, our results show that clusterin gene expression is induced in response to heat shock and oxidative stress in human A431 cells, and confers cellular protection against heat shock and oxidative stress.  (+info)

Effect of targeted expression of clusterin in photoreceptor cells on retinal development and differentiation. (4/535)

Clusterin expression is increased in tissues undergoing apoptosis, including neurodegenerative retina, but the causal relationships remain to be clarified. To test the hypothesis that overexpression of clusterin could induce apoptosis in neurons, transgenic mice were generated in which rat clusterin transgene was expressed in photoreceptor cells under the transcriptional control of the human interphotoreceptor retinoid-binding protein (IRBP) promoter. Photoreceptor cell death in the resulting transgenic mice was examined by histology and TUNEL techniques. The expression of the clusterin transgene was confirmed by in situ hybridization in the photoreceptor cells, and results in a complex pattern of clusterin protein distribution in the retina. A reduction in apoptotic staining in the transgenic retinas was observed from birth to postnatal day 15. These results suggest that clusterin is not causally involved in apoptotic mechanisms of photoreceptor cell death, but may relate to cytoprotective functions.  (+info)

Nerve growth factor and epidermal growth factor stimulate clusterin gene expression in PC12 cells. (5/535)

Clusterin (apolipoprotein J) is an extracellular glycoprotein that might exert functions in development, cell death and lipid transport. Clusterin gene expression is elevated at sites of tissue remodelling, such as differentiation and apoptosis; however, the signals responsible for this regulation have not been identified. We use here the clusterin gene as a model system to examine expression in PC12 cells under the control of differentiation and proliferation signals produced by nerve growth factor (NGF) and by epidermal growth factor (EGF) respectively. NGF induced clusterin mRNA, which preceded neurite outgrowth typical of neuronal differentiation. EGF also activated the clusterin mRNA, demonstrating that both proliferation and differentiation signals regulate the gene. To localize NGF- and EGF-responsive elements we isolated the clusterin promoter and tested it in PC12 cell transfections. A 2.5 kb promoter fragment and two 1.5 and 0.3 kb deletion mutants were inducible by NGF and EGF. The contribution to this response of a conserved activator protein 1 (AP-1) motif located in the 0.3 kb fragment was analysed by mutagenesis. The mutant promoter was not inducible by NGF or EGF, which identifies the AP-1 motif as an element responding to both factors. Binding studies with PC12 nuclear extracts showed that AP-1 binds to this sequence in the clusterin promoter. These findings suggest that NGF and EGF, which give differential gene regulation in PC12 cells, resulting in neuronal differentiation and proliferation respectively, use the common Ras/extracellular signal-regulated kinase/AP-1 signalling pathway to activate clusterin expression.  (+info)

Clusterin (SGP-2) gene expression is cell cycle dependent in normal human dermal fibroblasts. (6/535)

In confluent human dermal fibroblasts brought to quiescence (G0) by serum starvation, the S phase peaked at 24 h after serum re-addiction and G2/M phase peaked at 36 h. This was confirmed by titration of h-gas1 mRNA (a marker of G0 phase) and histone H3 (a marker of S phase). Clusterin mRNA accumulation progressively increased in cells proceeding to confluence after seeding and to quiescence upon serum starvation, and peaked at around G0, in parallel with h-gas1 mRNA. At 6 h (roughly G1 phase) clusterin transcript formed a second peak, followed by a gradual decrease until 36 h. Correspondence of clusterin protein accumulation to its mRNA occurred solely with regard to the G0 peak but not to the second one. The possible meaning of the cell cycle related clusterin gene expression is discussed.  (+info)

Isolation of Ku70-binding proteins (KUBs). (7/535)

DNA-dependent protein kinase (DNA-PK) plays a critical role in resealing DNA double-stand breaks by non-homologous end joining. Aside from DNA-PK, XRCC4 and DNA ligase IV, other proteins which play a role(s) in this repair pathway remain unknown; DNA-PK contains a catalytic subunit (DNA-PKcs) and a DNA binding subunit (Ku70 and Ku80). We isolated Ku70-binding proteins (KUB1-KUB4) using yeast two-hybrid analyses. Sequence analyses revealed KUB1 to be apolipoprotein J (apoJ), also known as X-ray-inducible transcript 8 (XIP8), testosterone-repressed prostate message-2 (TRPM-2) and clusterin. KUB2 is Ku80. KUB3 and KUB4 are unknown, >10 kb trans-cripts. Interactions of apoJ/XIP8 or KUB3 with Ku70 were confirmed by co-immunoprecipitation analyses in MCF-7:WS8 breast cancer or IMR-90 normal lung fibroblast cells, respectively. The interaction of apoJ/XIP8 with Ku70 was confirmed by far-western analyses. Stable over-expression of full-length apoJ/XIP8 in MCF-7:WS8 caused decreased Ku70/Ku80 DNA end binding that was restored by apoJ/XIP8 monoclonal antibodies. The role of apoJ/XIP8 in ionizing radiation resistance/sensitivity is under investigation.  (+info)

Targeted disruption of the murine lecithin:cholesterol acyltransferase gene is associated with reductions in plasma paraoxonase and platelet-activating factor acetylhydrolase activities but not in apolipoprotein J concentration. (8/535)

Lecithin:cholesteryl acyltransferase (LCAT) deficiency resulting from targeted disruption of the Lcat gene in the mouse is associated with dramatic decreases in HDL concentration and the accumulation of nascent HDL in the plasma. We examined whether LCAT deficiency in mice is associated with a concomitant decrease in two antioxidative enzymes, paraoxonase (PON) and platelet-activating factor acetylhydrolase (PAF-AH). In control Lcat (+/+) mice both these enzymes are transported on HDL. Compared to Lcat (+/+) mice, HDL-cholesterol is reduced 94% and apoA-I, 90%, in Lcat (-/-) mice; this reduction in HDL is paralleled by a 71% decrease in PAF-AH activity and in a 58% decrease in PON activity. Apolipoprotein J (apoJ) levels, rather than being decreased, were significantly (P = 0.01) higher (36%) in Lcat (-/-) than in Lcat (+/+) mice, and the apo J/PON ratio was 3-fold greater in Lcat (-/-) than in Lcat (+/+) animals. Even though apolipoprotein A-I (apoA-I) concentration and PON activity were drastically reduced, there was no reduction in apoA-I and PON liver mRNA levels suggesting that post-transcriptional events are responsible for the reduction of plasma PON and apoA-I levels. Fast protein liquid chromatography (FPLC) revealed that in Lcat (+/+) mice both PON and PAF-AH activity is associated with large, apoA-I-containing HDL particles (9.7 nm by non-denaturing gradient gel electrophoresis) while in Lcat (-/-) mice both enzymes are associated with small 8.2 nm particles. We conclude that the concomitant reduction in HDL and apoA-I concentrations and PON and PAF-AH activities is best explained by rapid clearance of the small HDL particles found in LCAT deficiency.  (+info)

*Clusterin

"Entrez Gene: clusterin". Koltai T (2014). "Clusterin: a key player in cancer chemoresistance and its inhibition". OncoTargets ... In humans, clusterin is encoded by the CLU gene on chromosome 8. CLU is a molecular chaperone responsible for aiding protein ... Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and ... Wei L, Xue T, Wang J, Chen B, Lei Y, Huang Y, Wang H, Xin X (Aug 2009). "Roles of clusterin in progression, chemoresistance and ...

*CLUAP1

Clusterin associated protein 1, also known as CLUAP1, is a human gene. Model organisms have been used in the study of CLUAP1 ... clusterin associated protein 1". Retrieved 2011-08-30. "Radiography data for Cluap1". Wellcome Trust Sanger Institute. " ...

*GOLM1

Zhou Y, Li L, Hu L, Peng T (2010). "Golgi phosphoprotein 2 (GOLPH2/GP73/GOLM1) interacts with secretory clusterin". Mol Biol ...

*Low-density lipoprotein receptor-related protein 8

Bajari TM, Strasser V, Nimpf J, Schneider WJ (2003). "A model for modulation of leptin activity by association with clusterin ...

*Follicular dendritic cell sarcoma

Clusterin is a heterodimeric protein that aids in the clearance of cellular debris and is involved with apoptosis. Clusterin ... Grogg, Karen L; Macon, William R; Kurtin, Paul J; Nascimento, Antonio G (2004). "A survey of clusterin and fascin expression in ... These cells are best visualized with immunostaining using the FDC markers CD21, CD35, R4/23, clusterin, and KiM4p. Marker ... Characteristically FDCS have mircotubuloreticular structures (MTRS) and increased levels of intracellular clusterin. MTRS ...

*LRP2

"Identification of glycoprotein 330 as an endocytic receptor for apolipoprotein J/clusterin". J. Biol. Chem. 270 (22): 13070-5. ...

*NBPF1

Vandepoele K, Staes K, Andries V, van Roy F (April 2010). "Chibby interacts with NBPF1 and clusterin, two candidate tumor ...

*Human serum albumin

"Identification of Human Plasma Proteins as Major Clients for the Extracellular Chaperone Clusterin". J. Biol. Chem. 285 (6): ...

*Gene silencing

Antiapoptotic proteins, such as clusterin and survivin, are often expressed in cancer cells. Clusterin and survivin-targeting ... "Nucleotide-based therapies targeting clusterin chemosensitize human lung adenocarcinoma cells both in vitro and in vivo". ...

*Pathophysiology of multiple sclerosis

It appears together with clusterin and complement C3, markers of complement-mediated inflammatory reactions. Also Fibroblast ...

*Mothers against decapentaplegic homolog 3

Lee KB, Jeon JH, Choi I, Kwon OY, Yu K, You KH (February 2008). "Clusterin, a novel modulator of TGF-beta signaling, is ...

*Anaplastic large-cell lymphoma

Another useful marker which helps to differentiate this lesion from Hodgkin lymphoma is Clusterin. The neoplastic cells have a ...

*Albert Hofman

Plasma clusterin and the risk of Alzheimer disease. JAMA. 2011 Apr 6;305(13):1322-6. Solouki AM, et al. A genome-wide ...

*Reverse phase protein lysate microarray

9 (3). Proteome Science , Full text , Development of reverse phase protein microarrays for the validation of clusterin, a mid- ...

*Martin Gleave

Gleave's team was recognized in 2010 as the first in the world to develop an anti-clusterin agent. Richard D. Williams MD ...

*MMP25

2003). "Clusterin, an abundant serum factor, is a possible negative regulator of MT6-MMP/MMP-25 produced by neutrophils". J. ...

*Complement membrane attack complex

These fluid phase complexes do not bind to cell membranes and are ultimately scavenged by clusterin and vitronectin, two ...

*Metropolitan statistical area

CBSAs are delineated on the basis of a central urban area or urban cluster - in other words: a contiguous area of relatively ...

*SAT1 (gene)

... is accompanied by significant changes in the levels of expression of polyamine metabolism regulatory genes and clusterin ( ...

*Bronze Star Medal

Oak Leaf Cluster - In the Army and Air Force, the oak leaf cluster is worn to denote additional awards. 5/16 Inch Star - In the ...

*CLU

... a financial professional designation Clusterin Clu (Tron), fictional character from the Tron franchise Command Launch Unit for ...

*HeuristicLab

The following list gives an overview of the problems supported by HeuristicLab: Artificial Ant Classification Clusterin ...

*ACAMPs

Among soluble inhibitors there are factor H, C1 inhibitor, C4b-binding protein, factor I, S protein or clusterin, the membrane- ...

*Justin Yerbury

2007) The extracellular chaperone clusterin influences amyloid formation and toxicity by interacting with pre-fibrillar ...
Both isoforms of the hepatitis delta antigen (HDAg) of hepatitis delta virus (HDV) are highly associated with virus proliferation and may act as co-activators of cellular gene expression. Human hepatocellular carcinoma (HCC) cell line Huh7, which stably expresses HDAgs, was differentially screened and the results showed that clusterin gene expression was enhanced. The mechanisms for HDAg-mediated clusterin gene upregulation were investigated. Expression of HDAgs was associated with enhanced histone H3 acetylation within the clusterin promoter in a chromatin immunoprecipitation assay. Transient transfection of HDAg-expressing plasmids into Huh7 cells also enhanced clusterin expression and histone acetylation. Furthermore, HDV replication was associated with histone hyperacetylation and clusterin induction. The effect of increased clusterin expression was determined by a chemosensitivity assay with adriamycin treatment. These data indicated that HDV-induced clusterin protein increases cell survival
TY - JOUR. T1 - Interaction of complement and clusterin in renal injury. AU - Correa-Rotter, Ricardo. AU - Hostetter, Thomas H.. AU - Nath, Karl A. AU - Manivel, J. Carlos. AU - Rosenberg, Mark E.. PY - 1992/11. Y1 - 1992/11. N2 - Clusterin is a heterodimeric glycoprotein that has been associated with such diverse biologic functions as reproduction, cell regression, cell aggregation, and regulation of the cytolytic activity of the membrane attack complex of complement. Clusterin is a component of glomerular immune deposits in the kidney, and increased Clusterin expression occurs in a number of renal injury states. To further explore the interaction between Clusterin and complement, the requirement for an intact complement system for renal Clusterin induction in an acute (folic acid nephropathy) and a chronic (subtotal renal ablation) model of renal injury was examined. After it was first demonstrated that folic acid increased renal clusterin mRNA in the rat, a species in which renal clusterin ...
Background: Clusterin is a cytoprotective chaperone protein that promotes cell survival and confers broad-spectrum treatment resistance. OGX-011 is a 2′-methoxyethyl modified phosphorothioate antisense oligonucleotide that is complementary to clusterin mRNA and has been reported to inhibit clusterin expression and enhance drug efficacy in xenograft models. The primary objective of this clinical study was to determine a biologically effective dose of OGX-011 that would inhibit clusterin expression in human cancer. Methods: Subjects (n = 25) with localized prostate cancer with high-risk features who were candidates for prostatectomy were treated with OGX-011 by 2-hour intravenous infusion on days 1, 3, and 5 and then weekly from days 8-29 combined with androgen blockade starting on day 1; prostatectomy was performed on days 30-36. Six different doses were tested, from 40 to 640 mg. OGX-011 plasma and prostate tissue concentrations were measured by an enzyme-linked immunosorbent assay method, and ...
Purpose: The clusterin gene encodes a multi-functional protein that has been identified in different tissues, including a number of different eye tissues, primarily in the mouse and to a much lesser extent in humans. Clusterin has been implicated in a number of cellular processes such as lipid transport, membrane integrity, apoptosis, and neurodegeneration, all of which could be important to the biology of the eye. In the current communication, we provide data that confirms the expression of clusterin in a number of different human eye tissues and establishes the expression profile of this gene in monkey derived eye tissues. The issue that we sought to examine is whether a broad profile of clusterin expression in the eye is consistent in primates (monkey and human). Methods: The majority of our study was done using monkey eye tissues. Where possible, we have used human tissues in order to confirm published findings. Northern and western analysis was performed using tissues derived from monkey eyes. In
Antiestrogens represent the first line of therapy in the treatment of estrogen receptor-positive (ER+) breast cancer patients. Unfortunately, up to 40% of patients develop resistance associated with progression and frequently die for metastatic breast cancer. The molecular events leading to pharmacological resistance are not completely understood. We attempted to verify in an experimental model the role of cytoplasmic clusterin (CLU), a cytoprotective protein found to be up-regulated in antiestrogen-resistant patients, following neoadjuvant treatment with toremifene. The role of cytoplasmic clusterin in modulating response to two antiestrogens (toremifene and tamoxifen) was studied in two ER+ anti-estrogen-sensitive cell lines (MCF-7, 734B) and one ER+ antiestrogen-resistant cell line (T47D) using siRNA strategy. Resistant cells were characterised by higher levels of cytoplasmic clusterin than sensitive cells, and antiestrogen treatments up-regulated clusterin levels in both sensitive and ...
Apolipoprotein J Antibody functions as a secreted chaperone that prevents aggregation of nonnative proteins. It prevents stress-induced aggregation of blood plasma proteins and inhibits the formations of amyloid fibrils. Apolipoprotein J does not require ATP or refold proteins by itself. It maintains partially unfolded proteins in a state for subsequent refolding by other chaperones. It is shown to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Binding to cell surface receptors it triggers internalization of chaperone-client complex and subsequent lysosomal or proteasomal degradation. It modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis while mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Anti-Apolipoprotein J Antibody is useful for researchers interested in the immune system, Ubiquitin pathways, and cardiovascular research.
Clusterin is a chaperone protein that is synthesized in response to cellular stress. It often appears in cells undergoing apoptosis - both as part of developmental programmes such as tissue involution and in numerous pathological conditions - but has also been reported to confer resistance to apoptosis in some cases. Whether it is a pro-survival or a pro-death molecule is therefore unclear. Laure Debure and co-workers have examined the effects of clusterin expression in COS-7 cells (see p. 3109). They find that clusterin can accumulate in juxtanuclear aggregates that exhibit features characteristic of aggresomes (inclusion bodies thought to prevent misfolded proteins spreading throughout the cell). They have a vimentin cage, for example, and can be disrupted by treatment with the chaperone Hsp70 or microtubule-depolymerising drugs. The authors also observe, however, that clusterin expression disrupts mitochondria and induces apoptosis through the mitochondrial pathway - this can be prevented by ...
Clusterin was originally described as a major glycoprotein synthesized in the male reproductive systems of the ram and rat.1,2 Since then it has been identified in a wide range of biological fluids and tissues in many species.1,3-9 Identification of rat clusterin messenger ribonucleic acid (mRNA) to TRPM-2 (testosterone repressed prostatic message 2),10-12 a transcript found to be prevalent in vivo in involuting tissues whether induced in experimental models or naturally during development of the embryo, raised the question of a possible involvement of clusterin in programmed cell death.13,14 Reports by several independent groups of researchers on the upregulation of the clusterin gene in brains of hamsters infected with the scrapie agent15 and of humans afflicted with Alzheimers disease (AD),16 epilepsy,17 or gliomas,17 as well as in the degenerating human retina18 gave support to the apoptosis hypothesis and generated strong interest in the role of clusterin in the central nervous system ...
Immature motoneurons are highly susceptible to degeneration following axon injury. The response of perineuronal glia to axon injury may significantly influence neuronal survival and axon regeneration. We have examined the central reactions to neonatal facial nerve transection with emphasis on the expression of complement component C3 (C3) and the multifunctional apolipoprotein J (ApoJ). Axotomy was performed on one-day-old rats. Animals were perfused from eight hours to two weeks after the lesion. The astroglial marker, glial fibrillary acidic protein (GFAP) was increased from one day and the microglial marker OX-42 from two days after injury. ApoJ immunoreactivity was increased in axotomized neuronal perikarya and astroglial cells from one day postaxotomy, but no C3 immunoreactive profiles were found at any postoperative survival time. Cell proliferation as judged by bromodeoxyuridine labeling and immunoreactivity for the cyclin Ki-67 antigen (antibody MIB5) occurred only at two days after ...
Premature senescence of human diploid fibroblasts (HDFs) can be induced by exposures to a variety of oxidative stress and DNA damaging agents. In this study we developed a robust model of UVB-induced premature senescence of skin HDFs. After a series of 10 subcytotoxic (non-proapoptotic) exposures to UVB at 250 mJ/cm2, the so-called biomarkers of senescence were markedly expressed: growth arrest, senescence-associated β-galactosidase activity, senescence-associated gene overexpression, deletion in mitochondrial DNA. A set of 44 stress- and senescence-associated genes were found to be differentially expressed in this model, among which clusterin/apolipoprotein J (apo J) and transforming growth factor-β1 (TGF-β1). Transfection of apo J cDNA provided protection against premature senescence-inducing doses of UVB and other stressful agents. Neutralizing antibodies against TGF-β1 or its receptor II (TβRII) sharply attenuated the senescence-associated features, suggesting a role for TGF-β1 in ...
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Anti-human Clusterin mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c immunized mice.
Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified.
Figure 3. Ocular clusterin localization by immunohistochemistry and in situ analysis. Shown are sections of cornea (panels A-C, serial sections), skin/eyelid (panels D, E, serial sections), ciliary body (unfilled arrow and * in panel F), and lens (panels G, H, serial sections). Immunodetection with G7 are shown in panels B, E, F, and H; panels A, D, and G are the negative controls in which the G7 antibody was omitted. In situ hybridization of an anti-sense clusterin mRNA probe on cornea is shown in panel C. Panel I: graph illustrating the distribution of silver grain over different regions of the cornea shown in panel C, for each general region 10 independent counts within a fixed area were taken. Solid arrows indicate the interface between epithelial and stromal cells (cs) in the cornea, arrow heads indicate the area of epithelial cells in the lens.. ...
Loss of cardiomyocytes occurs with aging and contributes to cardiovascular complications. In the present study, we highlighted the role of clusterin, a protein that has recently been associated with the protection of cardiomyocytes from apoptosis. Clusterin protects cardiac cells against damage from myocardial infarction, transplant, or myocarditis. Clusterin can act directly or indirectly on apoptosis by regulating several intracellular pathways. These pathways include (1) the oxidant and inflammatory program, (2) insulin growth factor 1 (IGF-1) pathway, (3) KU70 / BCL-2-associated X protein (BAX) pathway, (4) tumor necrosis factor alpha (TNF-α) pathway, (5) BCL-2 antagonist of cell death (BAD) pathway, and (6) mitogen-activated protein kinase (MAPK) pathway ...
Joel 3:1-21. The Lord Will Be A Refuge Joel 3:1-21 Lesson 3 Key Verse: 3:16b JoelBibleSchool NIU UBF 5/29/08 Delivered by Jennifer Jesmer "…But the LORD will be a refuge for his people, a stronghold for the people of Israel." From the last passage I learned that repentance is the most important thing to […]. Read More... ...
This work has been made available to the staff and students of the University of Sydney for the purposes of research and study only. It constitutes material that is held by the University for the purposes of reporting for HERDC and the ERA. This work may not be downloaded, copied and distributed to any third party ...
As we can see the amount of discrete information held in working memory at any one time is quite limited. However, by writing things out as we process our information rather than simply listing the desired outcomes we can formulate a greater number of possibilities.. Lets look through our planners and pick a to-do. Let us suppose we wish to communicate good tidings to Macbeth and hail him Thane of Cawdor. The most obvious choice is to climb to the top of the highest tower and contact him via semaphore. However this would be time consuming not to mention hazardous. What are the options? By exploring the alternatives on paper we have provided ourselves with three choices each one conveys the main message- Macbeth you are now Thane of Cawdor plus a deeper more subjective one. For example:. * Carrier pigeons - Speedy yet impersonal.. * Messenger - slower but makes the receiver feel important.. * Three Fates wearing false beards appear out of thin air - dramatic; the sender is prone to ...
While I have always been extremely health conscious and am presently in excellent health, I did become temporarily out-of-commission (i.e. I was really sick) in 2005 with a number of at the time unexplainable symptoms. I was quite puzzled at the time because I had been eating mainly organically grown food, drinking spring water, doing Yoga every morning, and going to the gym several times a week. In other words, I was doing everything one is supposed to do to stay healthy. I was not supposed to get sick. It took me six months before discovering or even imagining the main source of the problem - which was in fact "overexposure to electromagnetic" - especially microwave - radiation. I was living within 200 meters of two cell phone towers at the time and within 500 meters of a 3rd one with numerous WiFi signals bleeding into my apartment from adjacent neighbors. I developed a host of symptoms, which are found in what has been misleadingly described as Chronic Fatigue Syndrome (CFS) -- but much more ...
Clusterin, ubiquitously distributed in mammalians, was cloned and identified as the most potently induced gene during rat prostate involution following androgen deprivation. Also found to be involved in many other patho-physiological processes, its biological significance is still controversial, particularly with regard to apoptosis. We previously showed that transient over-expression of clusterin blocked cell cycle progression of simian-virus-40-immortalized human prostate epithelial cell lines PNT1A and PNT2. We show in the present study that the accumulation of an intracellular 45 kDa clusterin isoform was an early event closely associated with death of PNT1A cells caused by cell detachment followed by apoptosis induction (anoikis). Cell morphological changes, decreased proliferation rate and cell cycle arrest at G0/G1-S-phase checkpoint were all strictly associated with the production and early translocation to the nucleus of a 45 kDa clusterin isoform. Later, nuclear clusterin was found ...
The CLU gene is located on p21-p12 of human chromosome 8, with CLU as its encoded product, which has various physiological functions, including participating in lipid metabolism (28), oxidative stress reaction (29), and cell cycle regulation (30). CLU is highly expressed in cerebrospinal fluid and amyloid plaques in brain tissues, and is involved in the pathogenesis of AD (4,5,31). Yerbury et al (32) demonstrated that the deposition of CLU in senile plaques and neurofibrillary tangles of AD. Howlett et al (33) further reported a correlation between CLU and senile plaque Aβ40 in the brain cortex of patients with AD. Martin-Rehrmann et al (34) demonstrated the presence of dysfunctional neurons with phosphorylated tau protein surrounding the senile plaques in 71% of CLU-positive patients with AD. Furthermore, they also showed that the tau and phosphorylated tau protein were significantly increased in the rat hippocampus, following the injection of a CLU-rich solution (34). It was suggested that ...
Objective: To investigate the differences between chondrocytes of the superficial and underlying zones of articular cartilage at the level of gene expression. Methods: Messenger RNA (mRNA) was isolated from chondrocytes harvested from the superficial and deep zones of immature bovine articular cartilage. This mRNA was reverse transcribed, radiolabeled, and then each complementary DNA (cDNA) sample was used to screen duplicate filters of a bovine chondrocyte cDNA library. By comparing autoradiographic signals on matching filter sets, clones exclusively expressed in the superficial zone of articular cartilage were isolated and characterized further. Results: Of the superficial-specific gene clones isolated, 25% were found to be a single gene product, clusterin. Northern hybridization was used to show that clusterin is expressed specifically in the superficial zone of articular cartilage and that its expression is up-regulated in mature cartilage. In situ hybridization was used to precisely ...
This is a randomized, double-blind, placebo-controlled, multicenter, international trial enrolling patients with metastatic CRPC who had a response to first-line docetaxel therapy and have prostate cancer-related pain with progression of disease. The intended intervention is second-line treatment with docetaxel retreatment or cabazitaxel plus study agent, where custirsen is to be administered in the investigational arm and placebo is to be administered in the control arm.. Selection of the chemotherapy (docetaxel re-treatment or cabazitaxel) is to be determined by the treating physician, based on the patients first-line response.. The study will primarily assess pain and analgesic use for evaluation of durable pain palliation in response to study treatment. Pain and analgesic use will be obtained via a 3rd party contact center (direct contact with patient).. Study treatment starts with a Loading Dose Period during which three infusions of study agent (custirsen vs. placebo) will be ...
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Expression of CLU (APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2) in oral mucosa tissue. Antibody staining with HPA000572 and CAB016253 in immunohistochemistry.
α2-Macroglobulin (α2M) is an extracellular chaperone that inhibits amorphous and fibrillar protein aggregation. The reaction of α2M with proteases results in an activated conformation, where the proteases become covalently-linked within the interior of a cage-like structure formed by α2M. This study investigates, the effect of activation on the ability of α2M to inhibit amyloid formation by Aβ1-42 and I59T human lysozyme and shows that protease-activated α2M can act via two distinct mechanisms: (i) by trapping proteases that remain able to degrade polypeptide chains and (ii) by a chaperone action that prevents misfolded clients from continuing along the amyloid forming pathway.
Interferon-alpha B2 (IFN-a8) (mPEG-ALD), 1 mg. Clusterin also names Apoliprotein J is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have
Mouse monoclonal antibody raised against partial recombinant CLU. Recombinant protein corresponding to a portion of the alpha subunit of human CLU. (MAB9601) - Products - Abnova
With age, forgetfulness and other signs of memory loss sometimes appear, prompting elderly individuals to seek a medical evaluation amid fears that they may be experiencing early symptoms of Alzheimers disease (AD), the ...
... Signed-off-by: Jens Axboe ,[EMAIL PROTECTED], --- drivers/scsi/aha1542.c , 32 +++++++++++++++----------------- 1 files changed, 15 insertions(+), 17 deletions(-) diff --git a/drivers/scsi/aha1542.c b/drivers/scsi/aha1542.c index cbbfbc9..961a188 100644 --- a/drivers/scsi/aha1542.c +++ b/drivers/scsi/aha1542.c @@ -61,15 +61,15 @@ static void BAD_DMA(void *address, unsigned int length) } static void BAD_SG_DMA(Scsi_Cmnd * SCpnt, - struct scatterlist *sgpnt, + struct scatterlist *sgp, int nseg, int badseg) { printk(KERN_CRIT sgpnt[%d:%d] page %p/0x%llx length %u\n, badseg, nseg, - page_address(sgpnt[badseg].page) + sgpnt[badseg].offset, - (unsigned long long)SCSI_SG_PA(&sgpnt[badseg]), - sgpnt[badseg].length); + page_address(sgp-,page) + sgp-,offset, + (unsigned long long)SCSI_SG_PA(sgp), + sgp-,length); /* * Not safe to continue. @@ -691,7 +691,7 @@ static int aha1542_queuecommand(Scsi_Cmnd * SCpnt, void (*done) (Scsi_Cmnd *)) ...
You are at: Home » Products » Health Projects » Environmental Rose Scented Envelope Sachet for clu In total 213268 number ofProductsinfo,Released today. 0 number of ...
Results Between ALS patients and healthy controls, there was significant difference in the expression of 30 proteins, including complement proteins and inflammatory markers. Of these, plasma Gelsolin concentration was 1.5 fold higher in healthy control in comparison with ALS patients (p=0.001). There were significant differences in 22 proteins between healthy controls and ALS patients with CI. Clusterin level was 1.2 fold upregulated in heathy people compared with patients with CI (p=0.03). Between ALS with and without CI, there was significant difference in expression of 25 proteins. CD5L concentration was significantly raised in patients without CI (p=0.013). The IPA shows that the complement pathway and coagulation pathway plays an important role in the ALS pathogenesis. ...
The goal of the present study was to determine whether treatment with cigarette smoke extract (CSE) induces cell loss, cellular senescence, and extracellular matrix (ECM) synthesis in primary human retinal pigment epithelial (RPE) cells. Primary cultured human RPE cells were exposed to 2, 4, 8, and 12% of CSE concentration for 24 hours. Cell loss was detected by cell viability assay. Lipid peroxidation was assessed by loss of cis-parinaric acid (PNA) fluorescence. Senescence-associated ß-galactosidase (SA-ß-Gal) activity was detected by histochemical staining. Expression of apolipoprotein J (Apo J), connective tissue growth factor (CTGF), fibronectin, and laminin were examined by real-time PCR, western blot, or ELISA experiments. The results showed that exposure of cells to 12% of CSE concentration induced cell death, while treatment of cells with 2, 4, and 8% CSE increased lipid peroxidation. Exposure to 8% of CSE markedly increased the number of SA-ß-Gal positive cells to up to 82%, and the mRNA
Expression of CLU (APOJ, CLI, CLU1, CLU2, KUB1, SGP-2, SP-40, TRPM-2) in vagina tissue. Antibody staining with HPA000572 and CAB016253 in immunohistochemistry.
I have an appointment with my favorite vet tomorrow to see what can be done for Tweezer. If he can still be made comfortable by anything I can do, then I will do everything I can for him. If there is nothing that can be done then it will be time to let him go with dignity. I can trust Dr. Tom to advise me correctly. Meanwhile I am a basket case. If Tweezer must crosst the bridge then a bright ray of sunshine will be leaving this world. -Original Message- From: [email protected] [mailto:[email protected]] On Behalf Of Natalie Sent: Thursday, February 03, 2011 3:50 PM To: [email protected] Subject: Re: [Felvtalk] HELP! Tweezer is sick I was giving her sub-q fluids for a while, which I assume helped her because she never had any seizures again. FIV is so much more manageable than FeLV! BTW - I sent the info to the dumb vet at the clinic so she would know for the next time someone brings in a cat that was FIV+, not to immediately make the diagnosis of ...
Demattos RB, ODell MA, Parsadanian M, Taylor JW, Harmony JA, Bales KR, Paul SM, Aronow BJ, Holtzman DM. Clusterin promotes amyloid plaque formation and is critical for neuritic toxicity in a mouse model of Alzheimers disease ...
Demattos RB, Cirrito JR, Parsadanian M, May PC, ODell MA, Taylor JW, Harmony JA, Aronow BJ, Bales KR, Paul SM, Holtzman DM. ApoE and clusterin cooperatively suppress Abeta levels and deposition: evidence that ApoE regulates extracellular Abeta metabolism in vivo ...
A phase III ITT controlled trial of Cbz/P/C in pts with previously-treated metastatic, castration-resistant PC shown no significant survival gain
Progression Free Survival: time from date of randomization to first objective documented progression per Response Evaluation Criteria In Solid Tumors (RECIST) v1.1 or death due to any cause, whichever occurs first. Tumor lesions measured in at least one dimension with minimum size of 10 mm by CT scan, 10 mm caliper by clinical exam. Malignant lymph nodes must be ,15 mm in short axis when assessed by CT scan. All measurable lesions up to a maximum of 2 lesions per organ and 5 in total representative of all involved organs should be identified as target lesions and measured and recorded ...
A study carried out with a new human stem cell-derived model reveals that the most prevalent genetic risk factor of Alzheimers disease (AD), apolipoprotein E4 (APOE4), impairs the function of human brain immune cells, microglia.
Background: Bridging integrator 1 (BIN1 ) has been identified as one of the most associated loci for Alzheimers disease (AD), and recently was reported to modulate tau pathology to mediate AD in vitro . However, the effects of BIN1 on the AD related
Protein expressions of Prx II and CLU in plasma by Western blot analysis. A: Single samples of normal and liver fibrosis. Immunoblotting with Prx II or CLU poly
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
Late-onset Alzheimers disease (AD) accounts for the majority of AD cases. A characteristic feature of AD is the accumulation of β-amyloid (Aβ) plaques in the brain. Several mutations are associated with risk for late-onset AD, particularly mutations in the genes encoding apolipoproteins APOE4 and clusterin (also known as APOJ), which alter the metabolism and impair the clearance of Aβ. Mutations in the gene encoding TREM2 (triggering receptor expressed on myeloid cells 2), a protein with a single transmembrane domain that is present in microglia in the brain, are also associated with increased risk of AD. Yeh et al. found that the three risk factors are mechanistically linked. A protein microarray screen using a library of secreted ligands immobilized on glass slides and a fusion antibody construct containing the extracellular domain of TREM2 or TREM1 identified various lipoproteins as potential selective ligands of TREM2. These included low-density lipoprotein, lipidated APOE and lapidated ...
Infants and girlish children may find assessment of the orifice and markedly the pharynx and uvula to be noticeably intrusive, so potter that portion of the assessment until the outstrip of the examination, after otoscopic evaluation. Laboratory and diagnostic testing may contain: В· CT scan or MRI to determine the size of the lesion and to identify metastasis В· Bone con to judge the enormousness of malignancy Nursing Government The minor wishes conventionally be honestly anxious with the feasibility of amputation and regular upon the limb salvage procedure. Heres around of his articles http://www.sc-coroners.org/interactive/indication7/directive6/ breast cancer wigs. Repeated TMS can yield potent effects that outlast the interval of stimula- tion, constraint with stimulation at give 1 Hz, and excitation with stimulation at 5 Hz and higher. Particular expression of clusterin (SGP-2) and outfit C1qB and C4 during responses to neurotoxins in vivo and in vitro. This is the almost wide ...
CLU Antibody (monoclonal) (M01), Mouse monoclonal antibody raised against a partial recombinant CLU. validated in WB, E (AT1564a), Abgent
dlls/secur32/schannel.c , 27 +++++++++++++++++++++++++-- 1 files changed, 25 insertions(+), 2 deletions(-) diff --git a/dlls/secur32/schannel.c b/dlls/secur32/schannel.c index 70458fa..0e8b9e2 100644 --- a/dlls/secur32/schannel.c +++ b/dlls/secur32/schannel.c @@ -1187,6 +1187,19 @@ static int schan_validate_decrypt_buffer_desc(PSecBufferDesc message) return data_idx; } +static void schan_decrypt_fill_buffer(PSecBufferDesc message, ULONG buffer_type, void *data, ULONG size) +{ + int idx; + SecBuffer *buffer; + + idx = schan_find_sec_buffer_idx(message, 0, SECBUFFER_EMPTY); + buffer = &message-,pBuffers[idx]; + + buffer-,BufferType = buffer_type; + buffer-,pvBuffer = data; + buffer-,cbBuffer = size; +} + static SECURITY_STATUS SEC_ENTRY schan_DecryptMessage(PCtxtHandle context_handle, PSecBufferDesc message, ULONG message_seq_no, PULONG quality) { @@ -1198,6 +1211,7 @@ static SECURITY_STATUS SEC_ENTRY schan_DecryptMessage(PCtxtHandle context_handle ssize_t received = 0; ssize_t ret; int idx; + ...
As databases continue to grow in size, efficient and effective clustering algorithms play a paramount role in data mining applications. Practical clusterin
Sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin. Also binds to collagen IV and perlecan. It probably has a role in cell-extracellular matrix interactions.
CLU Full-Length MS Protein Standard (NP_976084), Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine, was produced in human 293 cells (HEK293) with fully chemically defined cell culture medium to obtain incorporation efficiency at Creative-Proteomics. The protein encoded by this gene is a secreted chaperone that can under some stress conditions also be found in the cell cytosol. It has been suggested to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Alternate splicing results in both coding and non-coding variants.
This study is for patients with cancer of the prostate gland that has metastasized or spread outside the prostate to other parts of the body. Patients h
PICALM, the gene encoding phosphatidylinositol-binding clathrin assembly (picalm) protein, was recently shown to be associated with risk of Alzheimer disease (AD). Picalm is a key component of clathrin-mediated endocytosis. It recruits clathrin and adaptor protein 2 (AP-2) to the plasma membrane and, along with, AP-2 recognizes target proteins. The attached clathrin triskelions cause membrane deformation around the target proteins enclosing them within clathrin-coated vesicles to be processed in lysosomes or endosomes. We examined the distribution of picalm in control and AD brain tissue and measured levels of picalm messenger RNA (mRNA) by real-time polymerase chain reaction. Immunolabeling of brain tissue showed that picalm is predominately present in endothelial cells. This was further supported by the demonstration of picalm in human cerebral microvascular cells grown in culture. Picalm mRNA was elevated in relation to glyceraldehyde-3-phosphate dehydrogenase but not factor VIII-related ...
PICALM, BIN1, CLU, and APOE are top candidate genes for Alzheimers disease, and they influence episodic memory performance in old age. Physical activity, however, has been shown to protect against age-related decline and counteract genetic influences on cognition. The aims of this study were to assess whether (a) a genetic risk constellation of PICALM, BIN1, and CLU polymorphisms influences cognitive performance in old age; and (b) if physical activity moderates this effect. Data from the SNAC-K population-based study were used, including 2,480 individuals (age range = 60 to 100 years) free of dementia at baseline and at 3- to 6-year follow-ups. Tasks assessing episodic memory, perceptual speed, knowledge, and verbal fluency were administered. Physical activity was measured using self-reports. Individuals who had engaged in frequent health-or fitness-enhancing activities within the past year were compared with those who were inactive. Genetic risk scores were computed based on an integration of ...
Texas inmate Yokamon Hearn is scheduled to be executed today, and though he will be the states sixth prisoner executed this year, he will be the first to be administered a new single-drug injection. Texas announced last week it would start using a single dose of pentobarbital, instead of using the sedative in combination with two other drugs. Other states have made the change and a number of courts have upheld the practice, despite death penalty opponents claims that the single dose causes prisoners to take longer to die than the three-drug combination. The Commercial Appeal has more.. In related news, officials in Georgia announced yesterday that they too were switching to single-drug executions. In that state, death row inmate Warren Lee Hill is set to be executed on Monday. His attorney says the states decision to change the drug so close to the execution date is troubling. WTVC News Channel 9 has more. ...
The amount of exercise required for protection was greater than that currently recommended by the Center for Disease Control (CDC) and various scientific organizations. They recommend a minimum of 75 minutes of vigorous exercise per week, such as running, or 150 minutes of moderate intensity exercise, such as brisk walking. These recommendations correspond to running between 4.6 and 7.7 miles per week.. Runners who ran over 15.3 miles per week, or over 2-times the recommended level, had over 40% lower risk for Alzheimers Disease mortality than those that were inadequately active. Running between 7.7 and 15.3 miles per week was associated with 25% lower risk compared to inadequate exercise, but this was not statistically significant. Merely meeting the CDC recommendation by running 4.6 to 7.7 miles per week was not associated with lower risk. Subjects who expended an equivalent amount of energy by walking appeared to enjoy the same risk reductions as running. However, one must walk about 50% ...
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
By Tony Forte,September 24, 2012,English For a change of pace, this months case study was submitted by my colleague, John Najarian, FALU, FLMI, CLU ChFC, VP and Chief Underwriter of our Group and Specialty Division. John is responsible for leading the underwriting of our Individual... Read More ...
The Badr lab is focused on studying the genetic drivers of malignancy and treatment resistance as well as developing targeted and experimental therapeutics for brain tumors.
Aspartame consumption is implicated in the development of obesity and metabolic disease despite the intention of limiting caloric intake. The mechanisms responsible for this association remain unclear, but may involve circulating metabolites and the gut microbiota. Aims were to examine the impact of chronic low-dose aspartame consumption on anthropometric, metabolic and microbial parameters in a…
Astronauts and workers in nuclear plants who repeatedly exposed to low doses of ionizing radiation (IR, ,10 cGy) are likely to incur specific changes in signal transduction and gene expression in various tissues of their body. Remarkable advances in high throughput genomics and proteomics technologies enable researchers to broaden their focus from examining single gene/protein kinetics to better understanding global gene/protein expression profiling and biological pathway analyses, namely Systems Biology. An ultimate goal of systems biology is to develop dynamic mathematical models of interacting biological systems capable of simulating living systems in a computer. This Glue Grant is to complement Dr. Boothmans existing DOE grant (No. DE-FG02-06ER64186) entitled "The IGF1/IGF-1R-MAPK-Secretory Clusterin (sCLU) Pathway: Mediator of a Low Dose IR-Inducible Bystander Effect" to develop sensitive and quantitative proteomic technology that suitable for low dose radiobiology researches. An improved ...
-- Avid Posts Record Revenue of $23.4 Million During Second Quarter FY 2017 with Contracted Backlog of Future Business Currently at $73 Million -- -- Beta-2 Glycoprotein-1 (β2GP1) Identified as a Biomarker that Correlates with Statistically Significant Improvement in Overall Survival for Patients Receiving the Bavituximab Combination ...
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Hepatitis delta virus epigenetically enhances clusterin expression via histone acetylation in human hepatocellular carcinoma...Hepatitis delta virus epigenetically enhances clusterin expression via histone acetylation in human hepatocellular carcinoma...

These data indicated that HDV-induced clusterin protein increases cell survival potential. Thus, it is possible that epigenetic ... Expression of HDAgs was associated with enhanced histone H3 acetylation within the clusterin promoter in a chromatin ... Transient transfection of HDAg-expressing plasmids into Huh7 cells also enhanced clusterin expression and histone acetylation. ... Furthermore, HDV replication was associated with histone hyperacetylation and clusterin induction. The effect of increased ...
more infohttps://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.007211-0

Recombinant Mouse Clusterin protein (ab194035) | AbcamRecombinant Mouse Clusterin protein (ab194035) | Abcam

Buy our Recombinant Mouse Clusterin protein. Ab194035 is a full length protein produced in HEK 293 cells and has been validated ...
more infohttps://www.abcam.com/recombinant-mouse-clusterin-protein-ab194035.html

Clusterin Human | ProSpecClusterin Human | ProSpec

Clusterin also named Apoliprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N- ... A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone ... Human native Clusterin was filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.1M phosphate buffer, 0.15M NaCl pH 7.5. ... Clusterin also named Apolipoprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N- ...
more infohttps://www.prospecbio.com/Clusterin_Human

Clusterin Canine | ProSpecClusterin Canine | ProSpec

A genuine function of clusterin has not been defined. One tempting hypothesis says that clusterin is an extracellular chaperone ... Canine Clusterin was lyophilized from PBS, pH 7.5.. Solubility. Add deionized H2O to a working volume of 0.5mg/ml and let the ... Clusterin also named Apolipoprotein J (APO-J) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N- ... Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations ...
more infohttps://www.prospecbio.com/Clusterin_Canine

Barrie Knitwear deploys Fujitsus Cluster-in-a-Box - Fujitsu NetherlandsBarrie Knitwear deploys Fujitsu's Cluster-in-a-Box - Fujitsu Netherlands

Fujitsu Cluster-in-a-Box is a true plug and play solution that can be installed and configured in less than 30 minutes, ... "Fujitsus Cluster-in-a-Box is a resilient, reliable and flexible platform that will support our business and growth... It ... Working with IT partner, JayByJay, it decided to deploy Fujitsus Cluster-in-a-Box - a high availability, all-in-one appliance ...
more infohttps://www.fujitsu.com/nl/about/resources/case-studies/070414_CS_2014Jun_BarrieKnitwear.html

Distribution of clusterin in Alzheimer brain tissue.  - PubMed - NCBIDistribution of clusterin in Alzheimer brain tissue. - PubMed - NCBI

... but only antibodies to clusterin and vitronectin strongly stained amyloid deposits in senile plaques. The clusterin antibody ... Clusterin, vitronectin and protectin are all believed to inhibit membrane insertion by the MAC, and these data are consistent ... Distribution of clusterin in Alzheimer brain tissue.. McGeer PL1, Kawamata T, Walker DG. ... The immunohistochemical distribution of clusterin (SP40,40, SGP-2) was determined in Alzheimer disease (AD) and normal human ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/1378350?dopt=Abstract

FUJITSU Integrated System PRIMEFLEX for Cluster-in-a-box - Fujitsu SwedenFUJITSU Integrated System PRIMEFLEX for Cluster-in-a-box - Fujitsu Sweden

PRIMEFLEX Cluster-in-a-box offers a well-balanced combination of server, storage, and networking components in one compact ... With PRIMEFLEX Cluster-in-a-box, customers receive a hyper-converged solution including Microsoft Windows Server 2012 R2 pre- ... PRIMEFLEX Cluster-in-a-box is perfectly suited for organizations with experience in administration of Microsoft Windows Server ... Designed for mid-market organizations and branch offices, PRIMEFLEX Cluster-in-a-box offers continuous availability at low ...
more infohttp://www.fujitsu.com/se/products/computing/integrated-systems/cluster-in-a-box.html

Clusterin - WikipediaClusterin - Wikipedia

"Entrez Gene: clusterin". Koltai T (2014). "Clusterin: a key player in cancer chemoresistance and its inhibition". OncoTargets ... In humans, clusterin is encoded by the CLU gene on chromosome 8. CLU is a molecular chaperone responsible for aiding protein ... Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and ... Wei L, Xue T, Wang J, Chen B, Lei Y, Huang Y, Wang H, Xin X (Aug 2009). "Roles of clusterin in progression, chemoresistance and ...
more infohttps://en.wikipedia.org/wiki/Clusterin

Clusterin/APOJ Antibody (NBP1-90128): Novus BiologicalsClusterin/APOJ Antibody (NBP1-90128): Novus Biologicals

Rabbit Polyclonal Anti-Clusterin/APOJ Antibody. Validated: IHC, IHC-P. Tested Reactivity: Human. 100% Guaranteed. ... Blogs on Clusterin/APOJ. There are no specific blogs for Clusterin/APOJ, but you can read our latest blog posts. ... Reviews for Clusterin/APOJ Antibody (NBP1-90128) (0) There are no reviews for Clusterin/APOJ Antibody (NBP1-90128). By ... PTMs for Clusterin/APOJ Antibody (NBP1-90128). Learn more about PTMs related to Clusterin/APOJ Antibody (NBP1-90128). ...
more infohttps://www.novusbio.com/products/clusterin-apoj-antibody_nbp1-90128

CiNii 論文 - 
 		
 		
 			
 		 	
 		 		
 		 			Expression of Clusterin in Human Pancreatic Cancer
 		 		
 		 		
 		 	
 		
 	CiNii 論文 - Expression of Clusterin in Human Pancreatic Cancer

Expression of Clusterin in Human Pancreatic Cancer * * XIE Min-Jue * Department of Internal Medicine and Medical Oncology, ... Human clusterin gene expression is confined to surviving cells during in vitro programmed cell death FRENCH LE ... Clusterin overexpression in rat pancreas during the acute phase of pancreatitis and pancreatic development CALVO EL ... Expression of clusterin in pancreatic acinar cell injuries in vivo and in vitro XIE M-J ...
more infohttps://ci.nii.ac.jp/naid/10013987807

JCI -
Apolipoprotein J/clusterin limits the severity of murine autoimmune myocarditisJCI - Apolipoprotein J/clusterin limits the severity of murine autoimmune myocarditis

Apolipoprotein J/clusterin (apoJ/clusterin), an intriguing protein with unknown function, is induced in myocarditis and ...
more infohttps://www.jci.org/articles/view/9037/figure/8

Human Clusterin DuoSet ELISA DY5874: R&D SystemsHuman Clusterin DuoSet ELISA DY5874: R&D Systems

Build your own Human Clusterin ELISA with R&D Systems DuoSet Development Kit. Assay Range: 63-4,000 pg/mL. Versatile, ... Background: Clusterin. Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40,40, is a ... Reviews for Human Clusterin DuoSet ELISA. There are currently no reviews for this product. Be the first to review Human ... Citations for Human Clusterin DuoSet ELISA. R&D Systems personnel manually curate a database that contains references using R&D ...
more infohttps://www.rndsystems.com/products/human-clusterin-duoset-elisa_dy5874

Purified anti-Clusterin Antibody  anti-Clusterin - A15113APurified anti-Clusterin Antibody anti-Clusterin - A15113A

Clusterin, also known as Apolipoprotein J, is a heterodimeric glycoprotein that is a member of the heat-shock protein family. ... Recombinant human clusterin. Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Preparation The ... Clusterin is a protein of 449 amino acids with a molecular mass of 52 kD. Distribution Tissue distribution: Ubiquitously ... Clusterin, also known as Apolipoprotein J, is a heterodimeric glycoprotein that is a member of the heat-shock protein family. ...
more infohttps://www.biolegend.com/en-us/products/purified-anti-clusterin-antibody-14010

Research Brief: Clusterin Grabs Spotlight Among Elite Few LOAD Genes | ALZFORUMResearch Brief: Clusterin Grabs Spotlight Among Elite Few LOAD Genes | ALZFORUM

... seemed to affect clusterin mRNA levels or protein expression in blood. Whether clusterin turns out to be a useful biomarker ... Research Brief: Clusterin Grabs Spotlight Among Elite Few LOAD Genes. Quick Links. *Article ... Association of plasma clusterin concentration with severity, pathology, and progression in Alzheimer disease. Arch Gen ... First author Ilyas Kamboh and colleagues confirmed the LOAD associations of clusterin and PICALM in a case-control sample of ...
more infohttps://www.alzforum.org/news/research-news/research-brief-clusterin-grabs-spotlight-among-elite-few-load-genes

Clusterin Mouse HEK293 | BioVendorClusterin Mouse HEK293 | BioVendor

Clusterin. By Technical Data. Clusterin - Proteins. Clusterin - Recombinant protein - Proteins. Mouse - Clusterin - Proteins. ... Clusterin - for Western blotting - Proteins. Clusterin - for ELISA - Proteins. Clusterin - for Cell culture and/or animal ... Clusterin is a heavily N-glycosylated protein.. Across a broad range of species clusterin shows 70% to 80% of sequence homology ... References to Clusterin. *Choi-Miura NH, Oda T: Relationship between multifunctional protein Clusterin and Alzheimer disease. ...
more infohttps://www.biovendor.com/clusterin-mouse-hek293

Clusterin: A potential target for improving response to antiestrogensClusterin: A potential target for improving response to antiestrogens

We therefore concluded that: i) basal clusterin levels are higher in antiestrogen resistant cells, ii) clusterin is up- ... Clusterin: A potential target for improving response to antiestrogens. *Authors: *Sara Toffanin ... We attempted to verify in an experimental model the role of cytoplasmic clusterin (CLU), a cytoprotective protein found to be ... Toffanin, S., Daidone, M. G., Miodini, P., De Cecco, L., Gandellini, P., Cappelletti, V.Clusterin: A potential target for ...
more infohttps://www.spandidos-publications.com/ijo/33/4/791

Plasma clusterin & risk of neurological diseases | MDedge NeurologyPlasma clusterin & risk of neurological diseases | MDedge Neurology

According to the findings of a community-based study, in younger patients higher clusterin levels involved a ________ risk of ...
more infohttps://www.mdedge.com/neurology/quiz/3303/alzheimers-cognition/plasma-clusterin-risk-neurological-diseases?channel=180

Clusterin (CLU) Polyclonal Antibody size: 50 μgClusterin (CLU) Polyclonal Antibody size: 50 μg

Clusterin CLU Polyclonal Antibody is available 28 times from supplier bioma at Gentaur.com shop ... Clusterin CLU Polyclonal Antibody. Clusterin CLU Polyclonal Antibody is available 24 times from Bioma labs CAU26409 , Clusterin ... CAU26411 , Clusterin (CLU) Polyclonal Antibody size: 100 μg , 607.70 USD CAU26413 , Clusterin (CLU) Polyclonal Antibody size: ... CAU26410 , Clusterin (CLU) Polyclonal Antibody size: 200 μg , 879.16 USD CAU26413 , Clusterin (CLU) Polyclonal Antibody size: ...
more infohttps://gentaur.com/1461742845/clusterin-clu-polyclonal-antibody/bioma?p=2012489292

Clusterin - an apoptotic puzzle | Journal of Cell ScienceClusterin - an apoptotic puzzle | Journal of Cell Science

Clusterin is a chaperone protein that is synthesized in response to cellular stress. It often appears in cells undergoing ... Laure Debure and co-workers have examined the effects of clusterin expression in COS-7 cells (see p. 3109). They find that ... Debure and co-workers reconcile these seemingly conflicting effects on cell survival by suggesting that clusterin is an anti- ... The authors also observe, however, that clusterin expression disrupts mitochondria and induces apoptosis through the ...
more infohttp://jcs.biologists.org/content/116/15/e1504

Clusterin Rat, Rabbit Polyclonal Antibody | BioVendorClusterin Rat, Rabbit Polyclonal Antibody | BioVendor

Clusterin. By Technical Data. Clusterin - Antibodies. Clusterin - Polyclonal Antibody - Antibodies. Rat - Clusterin - ... Clusterin - from Rabbit - Antibodies. Clusterin - for Western blotting - Antibodies. Clusterin - for ELISA - Antibodies. ... Clusterin is a heavily N-glycosylated protein.. Across a broad range of species clusterin shows 70% to 80% of sequence homology ... References to Clusterin. *Choi-Miura NH, Oda T: Relationship between multifunctional protein Clusterin and Alzheimer disease. ...
more infohttps://www.biovendor.com/clusterin-rat-rabbit-polyclonal-antibody

Clusterin Impairs Hepatic Insulin Sensitivity and Adipocyte Clusterin Associates With Cardiometabolic Risk | Diabetes CareClusterin Impairs Hepatic Insulin Sensitivity and Adipocyte Clusterin Associates With Cardiometabolic Risk | Diabetes Care

The relationship of clusterin gene expression and plasma clusterin with IR, cardiovascular biomarkers, and risk of ... Circulating human clusterin exhibited similar associations. In human adipocytes, palmitate enhanced clusterin secretion, and in ... Our objective was to determine 1) whether subcutaneous AT adipocyte (SAd) clusterin and serum clusterin are associated with ... Clusterin Impairs Hepatic Insulin Sensitivity and Adipocyte Clusterin Associates With Cardiometabolic Risk ...
more infohttp://care.diabetesjournals.org/content/early/2019/01/11/dc18-0870

Chapter 4 Regulation of Clusterin Activity by CalciumChapter 4 Regulation of Clusterin Activity by Calcium

In this chapter, the attention is put on Ca2+ effect on Clusterin (CLU) activity. We showed that two CLU forms (secreted and ...
more infohttps://insights.ovid.com/adcr/200901041/00000269-200901041-00004

Apolipoprotein J/Clusterin Prevents a Progressive Glomerulopathy of Aging | Molecular and Cellular BiologyApolipoprotein J/Clusterin Prevents a Progressive Glomerulopathy of Aging | Molecular and Cellular Biology

Apolipoprotein J/Clusterin Prevents a Progressive Glomerulopathy of Aging. Mark E. Rosenberg, Richard Girton, David Finkel, ... Apolipoprotein J/Clusterin Prevents a Progressive Glomerulopathy of Aging. Mark E. Rosenberg, Richard Girton, David Finkel, ... Apolipoprotein J/Clusterin Prevents a Progressive Glomerulopathy of Aging. Mark E. Rosenberg, Richard Girton, David Finkel, ... In the apoJ/clusterin-deficient mice, immune complexes of immunoglobulin G (IgG), IgM, IgA, and in some cases C1q, C3, and C9 ...
more infohttps://mcb.asm.org/content/22/6/1893.abstract

A Role for Clusterin in Exfoliation Syndrome and Exfoliation Glaucoma?A Role for Clusterin in Exfoliation Syndrome and Exfoliation Glaucoma?

The multifunctional protein clusterin (CLU) is a secreted glycoprotein ubiquitously expressed throughout the body, including in ... Clusterin is commonly identified at fluid-tissue interfaces, and has been identified in most body fluids. It is a component of ... Although CLU has been considered as a therapeutic target in AD, cancer and dry eye, a role for clusterin in XFS/XFG needs to be ... Interestingly, clusterin associates with altered elastic fibers in human photoaged skin and prevents UV-induced elastin ...
more infohttps://insights.ovid.com/jglau/201807001/00061198-201807001-00012

Induction of clusterin in tubules of nephrotic rats. | American Society of NephrologyInduction of clusterin in tubules of nephrotic rats. | American Society of Nephrology

Induction of clusterin in tubules of nephrotic rats.. R Correa-Rotter, M E Ibarra-Rubio, G Schwochau, C Cruz, J R Silkensen, J ... Induction of clusterin in tubules of nephrotic rats.. R Correa-Rotter, M E Ibarra-Rubio, G Schwochau, C Cruz, J R Silkensen, J ... Induction of clusterin in tubules of nephrotic rats.. R Correa-Rotter, M E Ibarra-Rubio, G Schwochau, C Cruz, J R Silkensen, J ... but there was no correlation between these cells and clusterin staining. In contrast to the extent and pattern of clusterin ...
more infohttps://jasn.asnjournals.org/content/9/1/33?ijkey=96b4c4ad607a9801a9f71cd2a93fb250f5aa04c2&keytype2=tf_ipsecsha
  • Immunohistochemistry-Paraffin: APOJ / Clusterin Antibody [NBP1-- Staining of human tonsil shows strong positivity in squamous epithelial cells. (novusbio.com)
  • Apolipoprotein J/clusterin (apoJ/clusterin), an intriguing protein with unknown function, is induced in myocarditis and numerous other inflammatory injuries. (jci.org)
  • Newkirk MM, Apostolakos P, Neville C and Fortin PR: Systemic lupus erythematosus, a disease associated with low levels of Clusterin/ApoJ, and anti-inflammatory protein. (biovendor.com)
  • Apoliprotein J (apoJ)/clusterin has attracted considerable interest based on its inducibility in multiple injury processes and accumulation at sites of remodeling, regression, and degeneration. (asm.org)
  • Aging mice deficient in apoJ/clusterin developed a progressive glomerulopathy characterized by the deposition of immune complexes in the mesangium. (asm.org)
  • Up to 75% of glomeruli in apoJ/clusterin-deficient mice exhibited moderate to severe mesangial lesions by 21 months of age. (asm.org)
  • In the apoJ/clusterin-deficient mice, immune complexes of immunoglobulin G (IgG), IgM, IgA, and in some cases C1q, C3, and C9 were detectable as early as 4 weeks of age. (asm.org)
  • In young apoJ/clusterin-deficient animals, the development of immune complex lesions was accelerated by unilateral nephrectomy-induced hyperfiltration. (asm.org)
  • Injected immune complexes localized to the mesangium of apoJ/clusterin-deficient but not wild-type mice. (asm.org)
  • These results establish a protective role of apoJ/clusterin against chronic glomerular kidney disease and support the hypothesis that apoJ/clusterin modifies immune complex metabolism and disposal. (asm.org)
  • Clusterin/apolipoprotein J (apoJ) is an extracellular chaperone involved in the quality control system against protein aggregation. (sigmaaldrich.com)
  • Clusterin is up- or down regulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. (prospecbio.com)
  • Furthermore, the researchers found elevated concentrations of plasma clusterin in AD transgenic mice that overexpress mutated amyloid precursor protein (APP) and presenilin 1, and higher clusterin mRNA levels in blood cells of AD patients, compared with MCI and control subjects. (alzforum.org)
  • seemed to affect clusterin mRNA levels or protein expression in blood. (alzforum.org)
  • One tempting hypothesis says that clusterin is an extracellular chaperone protecting cells from stress induced by degraded and misfolded protein precipitates.Clus-terin is up- or downregulated on the mRNA or protein level in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. (biovendor.com)
  • Clusterin mRNA was markedly induced in the kidneys of nephrotic rats (8.5-fold versus control). (asnjournals.org)
  • An increase in clusterin mRNA was not seen 1, 2, or 4 d after PAN injection. (asnjournals.org)
  • Meanwhile, the molecular size of clusterin mRNA detected in the array of tissues are identical in size, suggesting that the nature of the diversity in clusterin forms is due to post-translational modifications. (edu.au)
  • Morrissey C, Lakins J, Moquin A, Hussain M, Tenniswood M: An antigen capture assay for the measurement of serum Clusterin concentrations. (biovendor.com)
  • Our objective was to determine 1 ) whether subcutaneous AT adipocyte (SAd) clusterin and serum clusterin are associated with insulin resistance (IR) and known markers of cardiometabolic risk and 2 ) how clusterin may contribute to increased risk. (diabetesjournals.org)
  • The aim of this study was to investigate serum levels of lipocalin 2, clusterin, sTNFR-1, interleukin-6, homocysteine, and uric acid in patients and controls groups. (hindawi.com)
  • Clusterin has been ascribed a plethora of functions such as phagocyte recruitment, aggregation induction, complement attack prevention, apoptosis inhibition, membrane remodeling, lipid transport, hormone transport and/or scavenging, matrix metalloproteinase inhibition. (prospecbio.com)
  • Increased circulating levels of Clusterin enhance tumor aggressiveness by inhibiting apoptosis and by promoting epithelial to mesenchymal transition. (rndsystems.com)
  • The authors also observe, however, that clusterin expression disrupts mitochondria and induces apoptosis through the mitochondrial pathway - this can be prevented by expression of the pro-survival molecule Bcl-2 or inhibition of caspases. (biologists.org)
  • Clusterin has been implicated in a number of cellular processes such as lipid transport, membrane integrity, apoptosis, and neurodegeneration, all of which could be important to the biology of the eye. (edu.au)
  • Lipocalin 2, clusterin, soluble tumor necrosis factor receptor-1 (sTNFR-1), interleukin-6, homocysteine, and uric acid are inflammatory and/or biochemical markers. (hindawi.com)
  • Here, clusterin (CLU) was identified as a novel target gene of DEC1 and suppresses DNA damage-induced cell death in tumor cells. (aacrjournals.org)
  • Clusterin, vitronectin and protectin are all believed to inhibit membrane insertion by the MAC, and these data are consistent with upregulation of all three proteins in response to MAC formation in AD, and with a neuronal origin of clusterin. (nih.gov)
  • They find that clusterin can accumulate in juxtanuclear aggregates that exhibit features characteristic of aggresomes (inclusion bodies thought to prevent misfolded proteins spreading throughout the cell). (biologists.org)
  • In human adipocytes, palmitate enhanced clusterin secretion, and in human hepatocytes clusterin attenuated insulin signaling and APOA1 expression and stimulated hepatic gluconeogenesis. (diabetesjournals.org)
  • Analysis of conditioned media from RPE cells cultured on permeable supports suggests that different forms of clusterin display alternative patterns of secretion. (edu.au)
  • Clusterin is a chaperone protein that is synthesized in response to cellular stress. (biologists.org)
  • Debure and co-workers reconcile these seemingly conflicting effects on cell survival by suggesting that clusterin is an anti-apoptotic, aggresome-forming chaperone but can be cytotoxic if it accumulates. (biologists.org)
  • It is now widely accepted in the field that the normally secreted chaperone clusterin is redirected to the cytosol during endoplasmic reticulum (ER) stress, although the physiological function(s) of this physical relocation remain unknown. (edu.au)
  • We have shown that clusterin directly interacts with TDP-43 in vitro and potently inhibits its aggregation, and observed that in ER stressed neuronal cells, clusterin co-localized with TDP-43 and specifically reduced the numbers of cytoplasmic inclusions. (edu.au)
  • Clusterin exerts tissue protective functions in atherosclerosis and Alzheimer's disease by binding and neutralizing non-oxidatively modified LDL reduces cytotoxicity and beta-amyloid fibrils, respectively. (rndsystems.com)
  • Western analysis revealed that two major groups of clusterin exist in the eye, a high molecular weight group (>100 kDa) and a second group consisting of at least five clusterin species that are all approximately 80 kDa. (edu.au)
  • The purpose of this study was to examine the expression of clusterin in a disease model characterized early in its course by predominant glomerular injury. (asnjournals.org)
  • In contrast to the extent and pattern of clusterin staining, vimentin was seen in only sporadic, dilated tubules, in addition to its expected glomerular localization. (asnjournals.org)
  • Further studies have suggested that people who already have Alzheimer's disease have more clusterin in their blood, and that clusterin levels in blood correlate with faster cognitive decline in individuals with Alzheimer's disease, but have not found that clusterin levels predicted the onset of Alzheimer's disease. (wikipedia.org)
  • Distribution of clusterin in Alzheimer brain tissue. (nih.gov)
  • The immunohistochemical distribution of clusterin (SP40,40, SGP-2) was determined in Alzheimer disease (AD) and normal human brain tissue and compared with the distributions of vitronectin, protectin and the complement membrane attack complex (MAC). (nih.gov)
  • Clusterin is commonly identified at fluid-tissue interfaces, and has been identified in most body fluids. (ovid.com)
  • Localization of proliferating cell nuclear antigen, vimentin, c-Fos, and clusterin in the postischemic kidney. (jci.org)
  • Likewise, none of the PCNA or vimentin-positive cells expressed clusterin at detectable levels. (jci.org)
  • In the first, Simon Lovestone, King's College London, and colleagues report in this month's Archives of General Psychiatry that plasma levels of clusterin track with AD severity, pathology, and clinical progression. (alzforum.org)
  • Human native Clusterin was filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.1M phosphate buffer, 0.15M NaCl pH 7.5. (prospecbio.com)
  • Circulating human clusterin exhibited similar associations. (diabetesjournals.org)
  • Interestingly, clusterin associates with altered elastic fibers in human photoaged skin and prevents UV-induced elastin aggregation in vitro. (ovid.com)
  • We also showed that in Drosophila photoreceptor cells, clusterin co-expression gave ER stress-dependent protection against proteotoxicity arising from both Huntingtin-Q128 and mutant (R406W) human tau. (edu.au)
  • In the current communication, we provide data that confirms the expression of clusterin in a number of different human eye tissues and establishes the expression profile of this gene in monkey derived eye tissues. (edu.au)
  • The issue that we sought to examine is whether a broad profile of clusterin expression in the eye is consistent in primates (monkey and human). (edu.au)
  • Clusterin is expressed in a broad range of eye tissues in both human and monkey, suggesting that this is a characteristic feature in primates. (edu.au)
  • Clusterin associated protein 1, also known as CLUAP1, is a human gene. (wikipedia.org)
  • Treatment with siRNA completely abolished cytoplasmic clusterin expression in all cell lines, but its down-regulation resulted in a significant decrease of cell growth only in the resistant line. (spandidos-publications.com)
  • In response to Western diet feeding, an increase in adipocyte clusterin expression was associated with a progressive increase in liver fat, steatohepatitis, and fibrosis in aged LDLR −/− mice. (diabetesjournals.org)
  • We further showed that the expression of TDP-43 in transgenic Drosophila neurons induced ER stress and that co-expression of clusterin resulted in a dramatic clearance of mislocalized TDP-43 from motor neuron axons, partially rescued locomotor activity and significantly extended lifespan. (edu.au)
  • In addition, new insights were made in defining clusterin expression in ciliary body, cornea, and the retinal pigment epithelium. (edu.au)
  • Although there are many factors regarding multiple resistance that are still unknown, studies show that it may be mediated by different mechanisms, one of which is closely related to the expression of the clusterin (CLU) protein ( 2, 3 ). (aacrjournals.org)
  • Immunohistochemistry studies demonstrated clusterin primarily in tubules in the cortex and medulla. (asnjournals.org)
  • Clusterin, a marker for cell injury, was expressed primarily in the S3 segment and in the distal tubule with distinct staining patterns in each segment. (jci.org)
  • The clusterin gene encodes a multi-functional protein that has been identified in different tissues, including a number of different eye tissues, primarily in the mouse and to a much lesser extent in humans. (edu.au)
  • In the expanded cohort, Lovestone's team found that those with higher plasma clusterin levels had greater atrophy in the entorhinal cortex, more amyloid in the medial temporal lobe, and poorer performance on the Mini-Mental State Examination. (alzforum.org)
  • Two independent genome-wide association studies found a statistical association between a SNP within the clusterin gene and the risk of having Alzheimer's disease. (wikipedia.org)
  • CONCLUSIONS Adipocyte-derived clusterin is a novel ECM-related protein linking cardiometabolic disease and obesity through its actions in the liver. (diabetesjournals.org)
  • The appearance of clusterin precedes the development of tubulointerstitial disease and may be a response to the proteinuria. (asnjournals.org)
  • Clusterin (CLU) is an enigmatic molecule associated with various physiological processes and disease states. (pubmedcentralcanada.ca)
  • Clusterin and PICALM were two of the three genes identified in a pair of independent GWASs published in the same issue of Nature Genetics last year (see ARF related news story ). (alzforum.org)
  • Clusterin was first identified in ram rete testis fluid where it showed signs of clustering with rat sertoli cells and erythrocytes, hence its name. (wikipedia.org)
  • Resistant cells were characterised by higher levels of cytoplasmic clusterin than sensitive cells, and antiestrogen treatments up-regulated clusterin levels in both sensitive and resistant cell lines. (spandidos-publications.com)
  • We therefore concluded that: i) basal clusterin levels are higher in antiestrogen resistant cells, ii) clusterin is up-regulated following antiestrogen treatment independently of the sensitivity of the cell line, iii) down-regulation of cytoplasmic clusterin restores sensitivity to toremifene in the antiestrogen-resistant cell line. (spandidos-publications.com)
  • Increased numbers of proliferating tubular cells were seen at 6 d, but there was no correlation between these cells and clusterin staining. (asnjournals.org)
  • Clusterin is involved in many diseases related to oxidative stress and aging, including neurodegenerative disorders, cancers, diabetes, and inflammatory diseases. (biolegend.com)