Clustered Regularly Interspaced Short Palindromic Repeats
Inverted Repeat Sequences
Copies of nucleic acid sequence that are arranged in opposing orientation. They may lie adjacent to each other (tandem) or be separated by some sequence that is not part of the repeat (hyphenated). They may be true palindromic repeats, i.e. read the same backwards as forward, or complementary which reads as the base complement in the opposite orientation. Complementary inverted repeats have the potential to form hairpin loop or stem-loop structures which results in cruciform structures (such as CRUCIFORM DNA) when the complementary inverted repeats occur in double stranded regions.
CRISPR-Associated Proteins
Protein components of the CRISPR-CAS SYSTEMS for anti-viral defense in ARCHAEA and BACTERIA. These are proteins that carry out a variety of functions during the creation and expansion of the CRISPR ARRAYS, the capture of new CRISPR SPACERS, biogenesis of SMALL INTERFERING RNA (CRISPR or crRNAs), and the targeting and silencing of invading viruses and plasmids. They include DNA HELICASES; RNA-BINDING PROTEINS; ENDONUCLEASES; and RNA and DNA POLYMERASES.
CRISPR-Cas Systems
Adaptive antiviral defense mechanisms, in archaea and bacteria, based on DNA repeat arrays called CLUSTERED REGULARLY INTERSPACED SHORT PALINDROMIC REPEATS (CRISPR elements) that function in conjunction with CRISPR-ASSOCIATED PROTEINS (Cas proteins). Several types have been distinguished, including Type I, Type II, and Type III, based on signature motifs of CRISPR-ASSOCIATED PROTEINS.
RNA, Archaeal
Streptococcus thermophilus
RNA Cleavage
RNA, Guide
DNA, Intergenic
Sulfolobus solfataricus
DNA Cleavage
A reaction that severs one of the covalent sugar-phosphate linkages between NUCLEOTIDES that compose the sugar phosphate backbone of DNA. It is catalyzed enzymatically, chemically or by radiation. Cleavage may be exonucleolytic - removing the end nucleotide, or endonucleolytic - splitting the strand in two.
Interspersed Repetitive Sequences
Copies of transposable elements interspersed throughout the genome, some of which are still active and often referred to as "jumping genes". There are two classes of interspersed repetitive elements. Class I elements (or RETROELEMENTS - such as retrotransposons, retroviruses, LONG INTERSPERSED NUCLEOTIDE ELEMENTS and SHORT INTERSPERSED NUCLEOTIDE ELEMENTS) transpose via reverse transcription of an RNA intermediate. Class II elements (or DNA TRANSPOSABLE ELEMENTS - such as transposons, Tn elements, insertion sequence elements and mobile gene cassettes of bacterial integrons) transpose directly from one site in the DNA to another.
Repetitive Sequences, Nucleic Acid
Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Base Sequence
RNA, Bacterial
Archaea
One of the three domains of life (the others being BACTERIA and Eukarya), formerly called Archaebacteria under the taxon Bacteria, but now considered separate and distinct. They are characterized by: (1) the presence of characteristic tRNAs and ribosomal RNAs; (2) the absence of peptidoglycan cell walls; (3) the presence of ether-linked lipids built from branched-chain subunits; and (4) their occurrence in unusual habitats. While archaea resemble bacteria in morphology and genomic organization, they resemble eukarya in their method of genomic replication. The domain contains at least four kingdoms: CRENARCHAEOTA; EURYARCHAEOTA; NANOARCHAEOTA; and KORARCHAEOTA.
Pyrococcus furiosus
Sequence Analysis, DNA
Evolution, Molecular
Genetic Engineering
Plasmids
Multigene Family
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Bacteria
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
RNA
A polynucleotide consisting essentially of chains with a repeating backbone of phosphate and ribose units to which nitrogenous bases are attached. RNA is unique among biological macromolecules in that it can encode genetic information, serve as an abundant structural component of cells, and also possesses catalytic activity. (Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Trinucleotide Repeats
Tandem Repeat Sequences
Gene Transfer, Horizontal
The naturally occurring transmission of genetic information between organisms, related or unrelated, circumventing parent-to-offspring transmission. Horizontal gene transfer may occur via a variety of naturally occurring processes such as GENETIC CONJUGATION; GENETIC TRANSDUCTION; and TRANSFECTION. It may result in a change of the recipient organism's genetic composition (TRANSFORMATION, GENETIC).
Trinucleotide Repeat Expansion
An increased number of contiguous trinucleotide repeats in the DNA sequence from one generation to the next. The presence of these regions is associated with diseases such as FRAGILE X SYNDROME and MYOTONIC DYSTROPHY. Some CHROMOSOME FRAGILE SITES are composed of sequences where trinucleotide repeat expansion occurs.
Minisatellite Repeats
Tandem arrays of moderately repetitive, short (10-60 bases) DNA sequences which are found dispersed throughout the GENOME, at the ends of chromosomes (TELOMERES), and clustered near telomeres. Their degree of repetition is two to several hundred at each locus. Loci number in the thousands but each locus shows a distinctive repeat unit.
Ankyrin Repeat
Protein motif that contains a 33-amino acid long sequence that often occurs in tandem arrays. This repeating sequence of 33-amino acids was discovered in ANKYRIN where it is involved in interaction with the anion exchanger (ANION EXCHANGE PROTEIN 1, ERYTHROCYTE). Ankyrin repeats cooperatively fold into domains that mediate molecular recognition via protein-protein interactions.
Repetitive Sequences, Amino Acid
In defense of phage: viral suppressors of CRISPR-mediated adaptive immunity in bacteria. (1/73)
Viruses that infect bacteria are the most abundant biological agents on the planet and bacteria have evolved diverse defense mechanisms to combat these genetic parasites. One of these bacterial defense systems relies on a repetitive locus, referred to as a CRISPR (clusters of regularly interspaced short palindromic repeats). Bacteria and archaea acquire resistance to invading viruses and plasmids by integrating short fragments of foreign nucleic acids at one end of the CRISPR locus. CRISPR loci are transcribed and the long primary CRISPR transcript is processed into a library of small RNAs that guide the immune system to invading nucleic acids, which are subsequently degraded by dedicated nucleases. However, the development of CRISPR-mediated immune systems has not eradicated phages, suggesting that viruses have evolved mechanisms to subvert CRISPR-mediated protection. Recently, Bondy-Denomy and colleagues discovered several phage-encoded anti-CRISPR proteins that offer new insight into the ongoing molecular arms race between viral parasites and the immune systems of their hosts. (+info)Comparative analysis ofCas6b processing and CRISPR RNA stability. (2/73)
The prokaryotic antiviral defense systems CRISP R (clustered regularly interspaced short palindromic repeats)/Cas (CRISP Rassociated) employs short crRNAs (CRISP R RNAs) to target invading viral nucleic acids. A short spacer sequence of these crRNAs can be derived from a viral genome and recognizes a reoccurring attack of a virus via base complementarity. We analyzed the effect of spacer sequences on the maturation of crRNAs of the subtype I-B Methanococcus maripaludis C5 CRISP R cluster. The responsible endonuclease, termed Cas6b, bound non-hydrolyzable repeat RNA as a dimer and mature crRNA as a monomer. Comparative analysis of Cas6b processing of individual spacer-repeat-spacer RNA substrates and crRNA stability revealed the potential influence of spacer sequence and length on these parameters. Correlation of these observations with the variable abundance of crRNAs visualized by deep-sequencing analyses is discussed. Finally, insertion of spacer and repeat sequences with archaeal poly-T termination signals is suggested to be prevented in archaeal CRISP R/Cas systems. (+info)Protospacer recognition motifs: mixed identities and functional diversity. (3/73)
(+info)Diversity of CRISPR systems in the euryarchaeal Pyrococcales. (4/73)
(+info)Holding a grudge: persisting anti-phage CRISPR immunity in multiple human gut microbiomes. (5/73)
(+info)CRISPR-Cas: evolution of an RNA-based adaptive immunity system in prokaryotes. (6/73)
(+info)Probabilistic models for CRISPR spacer content evolution. (7/73)
(+info)CRISPR-spacer integration reporter plasmids reveal distinct genuine acquisition specificities among CRISPR-Cas I-E variants of Escherichia coli. (8/73)
(+info)
Restriction enzyme
For more detail, read CRISPR (Clustered regularly interspaced short palindromic repeats). In 2017, a group from University of ... Type III enzymes recognise short 5-6 bp-long asymmetric DNA sequences and cleave 25-27 bp downstream to leave short, single- ... Restriction Enzyme Database Portal: Biology (CS1: long volume value, Articles with short description, Short description is ... The inverted repeat palindrome is also a sequence that reads the same forward and backward, but the forward and backward ...
Samira Kiani
Her work combines Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) with synthetic biology. She is a 2019 AAAS ... Kiani works on safer gene therapies, including Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR). The Cas9 ... CS1 errors: missing periodical, Articles with short description, Short description matches Wikidata, Articles with hCards, ...
Cancer pharmacogenomics
Another new method being applied is Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). CRISPR, combined with ... A UGT1A1*28 allele means the presence of 7 TA repeats in the promoter sequence of the gene, instead of the normal 6 repeats. ... Moore CB, Guthrie EH, Huang MT, Taxman DJ (2010). "Short hairpin RNA (shRNA): design, delivery, and assessment of gene ... Lambeth LS, Smith CA (2013). "Short hairpin RNA-mediated gene silencing". SiRNA Design. Methods in Molecular Biology. Vol. 942 ...
De-extinction
These regions are called clustered regularly interspaced short palindromic repeats, otherwise known as CRISPR. Since the viral ... Portals: Biology Technology (Articles with short description, Short description is different from Wikidata, Articles needing ... cluster pine, Weeping willow and Acacia mearnsii. The thylacine, commonly known as the Tasmanian Tiger, was native to the ...
Philippe Horvath
Horvath explored sections in the bacterial genome with clustered regularly interspaced short palindromic repeats, both for ... clustered regularly interspaced short palindromic repeats). Early work was stimulated by with the aim of improving the ... Articles with short description, Short description is different from Wikidata, Pages using infobox person with multiple ...
CRISPR
... (/ˈkrɪspər/) (an acronym for clustered regularly interspaced short palindromic repeats) is a family of DNA sequences ... Nam KH, Kurinov I, Ke A (September 2011). "Crystal structure of clustered regularly interspaced short palindromic repeats ( ... June 2011). "Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed ... Hatoum-Aslan A, Maniv I, Marraffini LA (December 2011). "Mature clustered, regularly interspaced, short palindromic repeats RNA ...
Cas2
"Double-stranded endonuclease activity in Bacillus halodurans clustered regularly interspaced short palindromic repeats (CRISPR ... family of sequence-specific endoribonucleases associated with the clustered regularly interspaced short palindromic repeats". ... v t e (Articles with short description, Short description matches Wikidata, Protein pages needing a picture, Enzymes, All stub ...
DNA repair
A technology named clustered regularly interspaced short palindromic repeat (shortened to CRISPR-Cas9) was discovered in 2012. ... Articles with short description, Short description is different from Wikidata, Wikipedia indefinitely move-protected pages, Use ... MMEJ starts with short-range end resection by MRE11 nuclease on either side of a double-strand break to reveal microhomology ... In short, the process involves specialized polymerases either bypassing or repairing lesions at locations of stalled DNA ...
Guide RNA
CRISPR (Clustered regularly interspaced short palindromic repeats)/Cas9 is a technique used for gene editing and gene therapy. ... clustered regularly interspaced short palindromic repeats) with its associated Cas enzymes, as their adaptive immune system. ... Articles with short description, Short description is different from Wikidata, Articles needing cleanup from May 2021, All ... The insertions and deletions are mediated by short guide RNA (gRNAs) which encode the editing information in the form of ...
Non-coding RNA
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) are repeats found in the DNA of many bacteria and archaea. ... Articles with short description, Short description is different from Wikidata, Wikipedia introduction cleanup from January 2018 ... The repeats are separated by spacers of similar length. It has been demonstrated that these spacers can be derived from phage ... These RNA elements form one of two possible structures in regions encoding very short peptide sequences that are rich in the ...
Cas12a
CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) is named for the features of the invariant DNA sequences ... Articles with short description, Short description is different from Wikidata, Protein articles without symbol, Protein pages ...
Joyce Van Eck
"Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/ ... Joyce Van Eck publications indexed by Google Scholar (Articles with short description, Short description matches Wikidata, ...
CRISPR-Display
Clustered regularly interspaced short palindromic repeats) system for genome editing. The CRISPR/Cas9 system uses a short guide ... Articles with short description, Short description matches Wikidata, Articles needing additional references from March 2017, ... Xist A-repeat (RepA), and the 4,799-nt transcriptional activator HOTTIP. While all the constructs showed significant direct ...
Genome-wide CRISPR-Cas9 knockout screens
Clustered Regularly Interspaced Short Palindromic Repeats) Screen Identifies NEK7 as an Essential Component of NLRP3 ... The clustered regularly interspaced short palindrome repeats (CRISPR)/Cas9 system is a gene-editing technology that can ... the bacterial type II clustered regularly interspaced short palindrome repeats (CRISPR)/Cas9 system has become a simple and ... CS1 maint: multiple names: authors list, Articles with short description, Short description matches Wikidata, All articles with ...
Shredding (disassembling genomic data)
Clustered regularly interspaces short palindromic repeats(CRISPR) - Cas9 allows genetic material to be either altered, removed ... Shredding refers to the process in bioinformatics of taking assembled gene sequences and disassembling them into short ... sequences of usually 500 to 750 base pairs (bp). This is generally done for the purpose of taking the short shredded sequences ...
Francisco Mojica
Clustered Regularly Interspaced Short Palindromic Repeats). These were later developed into the first widespread genome editing ... proposing the acronym of Clustered Regularly Interspaced Short Palindromic Repeats to alleviate the confusion stemming from the ... Mojica, F.J.M.; Ferrer, C.; Juez, G.; RodrÃguez-Valera, F. (1995). "Long stretches of short tandem repeats are present in the ... "Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements". Journal of Molecular ...
Genome editing
CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are genetic elements that bacteria use as a kind of ... RNA-guided endonucleases:clustered regularly interspaced short palindromic repeats associated Cas9 (CRISPR/Cas9) are a new tool ... and the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system. Nine genome editors were available as ... meganucleases and the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system. Meganucleases, discovered ...
Trans-activating crRNA
In bacteria and archaea; CRISPR-Cas (clustered, regularly interspaced short palindromic repeats/CRISPR-associated proteins) ...
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing
A more recent method for genome editing uses CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) sequences and ... Articles with short description, Short description matches Wikidata, Orphaned articles from March 2016, All orphaned articles, ... Shorter oligos have lower hybridization energies, resulting in decreased stability of the oligo to the chromosomal target. Of ... Short sequence insertions are also possible using the SCAR-less method. In order to circumvent oligonucleotide length ...
CRISPR interference
... clustered regularly interspaced short palindromic repeats) pathway, the technique provides a complementary approach to RNA ... Articles with short description, Short description matches Wikidata, Genome editing, Repetitive DNA sequences, Non-coding RNA) ...
Gene knockout
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is a method for genome editing that contains a guide ... Articles with short description, Short description matches Wikidata, Genetically modified organisms, Molecular biology ... The DNA binding region consists of amino acid repeats that each recognize a single base pair of the desired targeted DNA ... This is done by introducing short sequences called loxP sites around the gene. These sequences will be introduced into the germ ...
Modifications (genetics)
Standing for 'Clustered Regularly Interspaced Short Palindromic Repeats', CRISPR gene editing allows scientists to manually ... Because the shorter-stemmed dandelions had higher fitness than long-stemmed dandelions and were able to survive more often, the ... This adaptation was possible due to a mutation occurring in a shorter-stemmed individual being selected by environmental ...
Human germline engineering
CRISPR-Cas9, which is short for clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9, is ... By using clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9, a gene editing technique, they disabled a gene ... Articles with short description, Short description matches Wikidata, Wikipedia neutral point of view disputes from October 2020 ...
Artificial transcription factor
The clustered regularly interspaced short palindromic repeats - Cas (CRISPR-Cas) system has been extensively studied to target ... Each repeating segment folds into two alpha helices and amino acids at residue positions 12 and 13 in the repeating segment ... Cis2/His2 zinc fingers have been extensively studied, are composed of 30 amino acids, can bind to non-palindromic sequences, ... Abnormal gene expression is regularly associated with cancer and uncontrolled tumor growth, making ATFs a promising therapeutic ...
Gene knockdown
Clustered Regularly Interspaced Short Palindromic Repeats', or CRISPRs. CRISPR-associated (cas) genes encode cellular machinery ... The CRISPR repeats are conserved amongst many species and have been demonstrated to be usable in human cells, bacteria, C. ... The transcripts of the short exogenous sequences are used as a guide to silence these foreign DNA when they are present in the ... The reduction can occur either through genetic modification or by treatment with a reagent such as a short DNA or RNA ...
Cas9
... is a dual RNA-guided DNA endonuclease enzyme associated with the Clustered Regularly Interspaced Short Palindromic Repeats ... CRISPR loci are composed of short, palindromic repeats that occur at regular intervals composed of alternate CRISPR repeats and ... Articles with short description, Short description is different from Wikidata, Articles to be expanded from September 2021, ... Essentially, when multiple repeat codons are produced, it elicits a response, or recruits an abundance of dCas9 to combat the ...
Kiana Aran
The chip integrates Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR) with single molecule graphene field- ... Articles with short description, Short description matches Wikidata, Orphaned articles from November 2021, All orphaned ...
Yoshizumi Ishino
... known for his discovering the DNA sequence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR). Ishino was ... Articles with short description, Short description is different from Wikidata, Articles with hCards, Articles containing ... "History of CRISPR-Cas from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology". Journal of Bacteriology ...
Biological warfare
A technique called Clustered, Regularly Interspaced, Short Palindromic Repeat (CRISPR-Cas9) is now so cheap and widely ... Articles with short description, Short description is different from Wikidata, Use dmy dates from September 2020, Articles ... Diseases such as wheat blast and rice blast were weaponized in aerial spray tanks and cluster bombs for delivery to enemy ... ISBN 978-2-37361-239-4. Carus WS (2017). A Short History of Biological Warfare: From Pre-History to the 21st Century. US ...
Ben Lamm
... "clustered regularly interspaced short palindromic repeats" (CRISPR) for Colossal's work on conservation biology. At its launch ... Articles with short description, Short description matches Wikidata, Articles with hCards, No local image but image on Wikidata ...
Cas1
... clustered regularly interspaced short palindromic repeats) in bacteria to adapt to new viral infections, identify the site in ... v t e (Articles with short description, Short description is different from Wikidata, Protein pages needing a picture, Enzymes ...
Human Nature (2019 film)
"Clustered Regularly Interspaced Short Palindromic Repeats"), and premiered in Austin, Texas at the South by Southwest film ... Official WebSite Human Nature at IMDb Portals: chemistry film science (Articles with short description, Short description is ...
Human genetic enhancement
By using clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9, a gene editing technique, they disabled a gene ... CS1 maint: bot: original URL status unknown, CS1: long volume value, Articles with short description, Short description matches ...
Lacticaseibacillus rhamnosus
... the clustered regularly interspaced short palindromic repeat (CRISPR) loci, and more than 100 transporter functions and mobile ... Therefore, the LRB genome is shorter than GG's genome. LRB lacks the spaCBA gene cluster of GG and is not expected to produce ... It is a short Gram-positive homofermentative facultative anaerobic non-spore-forming rod that often appears in chains. Some ... Type strain of Lactobacillus rhamnosus at BacDive - the Bacterial Diversity Metadatabase (Articles with short description, ...
Heterologous expression
Although CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats), a technique that allows for genes to be edited ... and short-term (transient). Long-term is a potentially permanent integration into the gene and short-term is a temporary ... This technique can be used for both short-term and long-term transfectants. It is also effective with almost any tissue type ... However, after identification of the corresponding active gene clusters, these genes can be cloned into yeast and expressed as ...
Designer baby
The CRISPR/Cas9 system (CRISPR - Clustered Regularly Interspaced Short Palindromic Repeats, Cas9 - CRISPR-associated protein 9 ... Articles with short description, Short description is different from Wikidata, 1989 introductions, 2018 introductions, ... An improved version of this technology, electron-avalanche transfection, has since been developed, which involves shorter ( ... This event is referred as Repeat Variable Diresidue (RVD). The relationship between the amino acids enables researchers to ...
Crispa
Clustered regularly interspaced short palindromic repeats), segments of prokaryotic DNA This disambiguation page lists articles ... Disambiguation pages with short descriptions, Short description is different from Wikidata, All article disambiguation pages, ...
MicrobesOnline
A tool for automatic detection of clustered regularly interspaced palindromic repeats". BMC Bioinformatics. 8: 209. doi:10.1186 ... Detection of CRISPRs: CRISPR are DNA loci involved in the immunity against invasive sequences, where short direct repeats are ... Edgar, R. C. (2007). "PILER-CR: Fast and accurate identification of CRISPR repeats". BMC Bioinformatics. 8: 18. doi:10.1186/ ... the cluster of orthologous groups (COGs) id, the GO term, the KEGG enzyme commission (EC) number, etc. as key words. The " ...
DARPA
detection system consisting of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based assays paired with ... Articles with short description, Short description matches Wikidata, Use American English from July 2019, All Wikipedia ...
Cellectis
With the advent of the CRISPR (clustered regularly interspaced short palindromic repeat) genome editing technique, the ... Pages containing links to subscription-only content, Articles with short description, Short description is different from ...
Off-target genome editing
... clustered, regularly interspaced, short palindromic repeats (CRISPR)-Cas9, transcription activator-like effector nucleases ( ...
CRISPR RNA
CRISPR-Cas (clustered, regularly interspaced short palindromic repeats - CRISPR associated systems) is an adaptive immune ... and 7 Is Required for the Biogenesis and Stability of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)- ... The CRISPR locus contains a series of repeats interspaced with unique spacers. These unique spacers can be acquired from MGEs. ... Mature crRNA transcripts contain a partial conserved section of repeat and a sequence of spacer that is complementary to the ...
Insight into microevolution of Yersinia pestis by clustered regularly interspaced short palindromic repeats - PubMed
Insight into microevolution of Yersinia pestis by clustered regularly interspaced short palindromic repeats Yujun Cui 1 , ... Insight into microevolution of Yersinia pestis by clustered regularly interspaced short palindromic repeats Yujun Cui et al. ... Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR), an element participating in immunity against phages ... is composed of short repeated sequences separated by unique spacers and provides the basis of the spoligotyping technology. In ...
Browse Releases: Oximoron EP, Prolonged Feeling of Nothingness, Clustered Regularly Interspaced Short Palindromic Repeats,...
Clustered Regularly Interspaced Short Palindromic Repeats-Cas
... Popular Genome Editing Tool Gets Its Close-Up Posted on March ... Tags: Cas9, Clustered Regularly Interspaced Short Palindromic Repeats-Cas, CRISPR-Cas, DNA editing, genome, Lurie Prize, Max ... Clustered Regularly Interspaced Short Palindromic Repeats-Cas), that researchers recently discovered could be put to use as a ...
Amplification of the Fluorescence Signal with Clustered Regularly Interspaced Short Palindromic Repeats-Cas12a Based on Au...
Amplification of the Fluorescence Signal with Clustered Regularly Interspaced Short Palindromic Repeats-Cas12a Based on Au ... sensitivity of Pb2+ detection is greatly enhanced via the use of a clustered regularly interspaced short palindromic repeats- ... Amplification of the Fluorescence Signal with Clustered Regularly Interspaced Short Palind ... cluster:("2002") AND pais_afiliacao:("^iUnited States^eEstados"))(instance:"regional") AND ( year_cluster:("2002") AND pais_ ...
Diagnostics | Free Full-Text | Molecular and Serological Tests for COVID-19. A Comparative Review of SARS-CoV-2 Coronavirus...
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas13) Technology. Recently, a test has been developed based ... on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas12a), with visual readouts. The test can detect 10 ... Taking samples of the condensate is safe, totally non-invasive, easy to perform, it may be repeated many times, it may also be ... BioMaxima has invented a test whose main advantages include a short waiting time; the results can be obtained in a mere 2 h. ...
Nucleic Acid Amplification Tests (NAATs) | CDC
Genetic screens reveal a central role for heme metabolism in artemisinin susceptibility
CRISPR (clustered regularly interspaced short palindromic repeats)\Cas (CRISPR associated proteins) systems serve seeing that...
Figure 1 - Fatal Necrotizing Enterocolitis in Neonate Caused by Cronobacter sakazakii Sequence Type 64 Strain of CRISPR...
Frontiers | SARS-CoV-2: The Monster Causes COVID-19
Clustered regularly interspaced short palindromic repeats; ddPCR, Droplet Digital PCR; HCoV-OC43, Human coronavirus OC43; HCoV- ... 2020). A Familial Cluster of Pneumonia Associated With the 2019 Novel Coronavirus Indicating Person-to-Person Transmission: A ... Study of a Family Cluster. Lancet 395, 514-523. doi: 10.1016/S0140-6736(20)30154-9 ...
CRISPR recognition tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats - PubMed
Clustered regularly interspaced short palindromic repeats (CRISPRs): the hallmark of an ingenious antiviral defense mechanism ... Background: Clustered Regularly Interspaced Palindromic Repeats (CRISPRs) are a novel type of direct repeat found in a wide ... CRISPR recognition tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats Charles ... CRISPR recognition tool (CRT): a tool for automatic detection of clustered regularly interspaced palindromic repeats Charles ...
NIH VideoCast - New Approaches to Detect, Study and Treat SARS-CoV-2 Infection
Gene Editing - Digital Media Kit | National Institutes of Health (NIH)
Publication Detail
Selection of highly efficient sgRNAs for CRISPR/Cas9-based plant genome editing | Scientific Reports
J. Efficient gene editing in tomato in the first generation using the clustered regularly interspaced short palindromic repeats ... More recently, the CRISPR (clustered regularly interspaced short palindromic repeat)-associated (Cas) endonuclease Cas9 has ... A characteristic CRISPR array consists of repetitive sequences (repeats) interspaced by short stretches of nonrepetitive ... 1B). The repeat and anti-repeat region (stem loop RAR) triggers precursor CRISPR RNA (pre-crRNA) processing by the enzyme RNase ...
Medicine's Future, From a Leader in Genome Editing and Stem Cells
Molecular Techniques | CRISPR/Cas9
GMH: Genetically Modified Humans? - Alliance for Natural Health USA - Protecting Natural Health
Biomarkers Search
6. Clustered Regularly Interspaced Short Palindromic Repeats System of Genome Engineering in Embryos to Repair Genes.. Niazvand ... 1. Advances in Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)-Based Diagnostic Assays Assisted by Micro/ ... 5. [Application of clustered regularly interspaced short palindromic repeats- associated protein 9 gene editing technology for ... 2. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas Advancement in Molecular Diagnostics and Signal ...
Comparative genome analysis of Streptococcus infantarius subsp. infantariusCJ18, an African fermented camel milk isolate with...
Genome decay is not as advanced as in S. thermophilus (10-19%) possibly due to a shorter history in dairy fermentations. ... Al-Attar S, Westra ER, van der Oost J, Brouns SJJ: Clustered regularly interspaced short palindromic repeats (CRISPRs): the ... Grissa I, Vergnaud G, Pourcel C: CRISPRFinder: a web tool to identify clustered regularly interspaced short palindromic repeats ... This is further supported by the apparent reduced activity of Clustered Regularly Interspaced Short Palindromic Repeats ( ...
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Hohman, Robert 2020 - Office of NIH History and Stetten Museum
Several years ago, a new technology came out, CRISPR-Cas9 [CRISPER= Clustered Regularly Interspaced Short Palindromic Repeats; ... She attended INRO in 2015 and was granted a post bac position in the EM Unit at RML, where in a short time she became a ... Hugh Auchincloss [NIAID Deputy Director and Acting Scientific Director] I developed a short Memo of Understanding (MOU) to ...
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Clustered Regularly Interspaced Short Palindromic Repeats; Cas: CRISPR-associated (protein) ... The column on the far left has the dates that the awards were given and the other column has a short description of the ... The three scenes are superimposed on an oval water mark to convey that these stages repeat themselves when clinical results ...
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Publications | Tanay Group
... and clustered regularly interspaced short palindromic repeats (CRISPR)-pooled screens. We show that profiling the genomic ... Here we introduce spatial clustering - a new unsupervised clustering methodology for dissection of large, multi-track genomic ... Analyses offered include low-level preprocessing and normalization, differential expression analysis, clustering, bi-clustering ... Spatial Clustering of Multivariate Genomic and Epigenomic Information. Jaschek R. & Tanay A. (2009) RESEARCH IN COMPUTATIONAL ...
CRISPR10
- CRISPR" refers to Clustered Regularly Interspaced Short Palindromic Repeats that occur in the genome of certain bacteria, from which the system was discovered. (biospace.com)
- Cas9 (CRISPR (clustered regularly interspaced short palindromic repeats)-associated protein-9 nuclease) nickase creates double-stranded breaks through two nuclease domains, named RuvC and HNH. (sigmaaldrich.com)
- A well-known one is called CRISPR-Cas9, which is short for clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9. (medlineplus.gov)
- They create a small piece of RNA with a short "guide" sequence that attaches (binds) to a specific target sequence in a cell's DNA, much like the RNA segments bacteria produce from the CRISPR array. (medlineplus.gov)
- Specifically, it harnesses the power of the gene-editing tool Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 to promote positive outcomes. (investorplace.com)
- The new study by Deem and graduate student Jiankui He focused on a portion of the bacterial genome called the 'CRISPR,' which stands for 'clustered regularly interspaced short palindromic repeats. (infectioncontroltoday.com)
- One type -- short, repeating patterns that first attracted scientific interest -- is what led to the CRISPR name. (infectioncontroltoday.com)
- In the present paper, we review the processing of CRISPR (clustered regularly interspaced short palindromic repeats) RNA, C/D box sRNA (small non-coding RNA) and tRNA in Nanoarchaeum equitans . (portlandpress.com)
- Secuencias repetitivas de ácidos nucleicos que son los principales componentes de los SISTEMAS CRISPR-CAS de arqueas y bacterias, que funcionan como sistemas adaptativos en la defensa frente a los virus. (bvsalud.org)
- Regions of archaeal (and bacterial) genomes containing a series of short repeated sequences separated by short unique sequences (CRISPR SPACERS). (bvsalud.org)
CRISPRs1
- The researchers named these sequences "clustered regularly interspaced short palindromic repeats," or CRISPRs for short. (nih.gov)
Sequences1
- The repeating sequences appear to be a kind of bookend or flag that the organism uses to determine where a snippet from a phage begins and ends. (infectioncontroltoday.com)
Sequence1
- An increase number of repeats of a genomic, tandemly repeated DNA sequence from one generation to the next. (bvsalud.org)
Spacers2
- But scientists more recently learned that the second type -- originally thought of as DNA 'spacers' between the repeats -- is what the organism uses to recognize disease. (infectioncontroltoday.com)
- These spacers, which are homologous to previously invading viruses, are generated from invading viral DNA and are inserted in between the repeats. (bvsalud.org)
Cells1
- Here we demonstrate that SFXN4 plays a role in synthesis of iron sulfur clusters (Fe-S) in ovarian cancer cells and ovarian cancer tumor-initiating cells, and that knockdown of SFXN4 inhibits Fe-S biogenesis in ovarian cancer cells. (nature.com)
Enzymes1
- Second, because enzymes critical to multiple DNA repair pathways require Fe-S clusters for their function, DNA repair enzymes and DNA repair itself are inhibited by reduction of SFXN4. (nature.com)
Data1
- TETyper: a bioinformatic pipeline for classifying variation and genetic contexts of transposable elements from short-read whole-genome sequencing data. (cdc.gov)
Technology1
- The Society of Toxicologic Pathology Career Development and Outreach Committee summary of a career development workshop on digital pathology, artificial intelligence, clustered regularly interspaced short palindromic repeats technology, microRNAs, and next-generation sequencing. (nih.gov)
