AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesTechnology, Industry, AgricultureInformation ScienceHealth Care
Clostridium thermocellumClostridiumCellulaseCelluloseCellulosomesCellobioseCellulose 1,4-beta-CellobiosidaseClostridium difficileXylosidasesClostridium InfectionsXylan Endo-1,3-beta-XylosidaseXylansbeta-GlucosidaseClostridium botulinumDextrinsEndo-1,4-beta XylanasesBacterial ProteinsTetrosesGlycoside HydrolasesMolecular Sequence Databeta-MannosidasePolynucleotide 5'-Hydroxyl-KinaseGlucansMultienzyme ComplexesAmino Acid SequenceHydrolysisFermentationSubstrate SpecificityCellulasesEnterocolitis, PseudomembranousGlucan 1,3-beta-GlucosidaseGenes, BacterialEthanolAcetylesteraseClostridium acetobutylicumCloning, MolecularDNA, BacterialBase SequenceSequence Homology, Amino AcidOrganellesClostridium tetaniEnzyme StabilityEscherichia coliDisaccharidesClostridium cellulolyticumTemperatureCrystallography, X-RayPolysaccharidesChromosomal Proteins, Non-HistoneBinding SitesBotulinum ToxinsProtein Structure, TertiaryHot TemperatureCarbohydrate MetabolismClostridium sordelliiSequence Analysis, DNAElectrophoresis, Polyacrylamide GelBacterial ToxinsMembrane ProteinsCrystallization
Clostridium thermocellum
A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.
Clostridium
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
Cellulase
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
Cellulose
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Cellulosomes
Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.
Cellobiose
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.
Cellulose 1,4-beta-Cellobiosidase
An exocellulase with specificity for the hydrolysis of 1,4-beta-D-glucosidic linkages in CELLULOSE and cellotetraose. It catalyzes the hydrolysis of terminal non-reducing ends of beta-D-glucosides with release of CELLOBIOSE.
Clostridium difficile
A common inhabitant of the colon flora in human infants and sometimes in adults. It produces a toxin that causes pseudomembranous enterocolitis (ENTEROCOLITIS, PSEUDOMEMBRANOUS) in patients receiving antibiotic therapy.
Xylosidases
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
Clostridium Infections
Infections with bacteria of the genus CLOSTRIDIUM.
Xylan Endo-1,3-beta-Xylosidase
A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.
Xylans
Polysaccharides consisting of xylose units.
beta-Glucosidase
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
Clostridium botulinum
A species of anaerobic, gram-positive, rod-shaped bacteria in the family Clostridiaceae that produces proteins with characteristic neurotoxicity. It is the etiologic agent of BOTULISM in humans, wild fowl, HORSES; and CATTLE. Seven subtypes (sometimes called antigenic types, or strains) exist, each producing a different botulinum toxin (BOTULINUM TOXINS). The organism and its spores are widely distributed in nature.
Dextrins
Endo-1,4-beta Xylanases
Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.
Bacterial Proteins
Proteins found in any species of bacterium.
Tetroses
Glycoside Hydrolases
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
beta-Mannosidase
An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.
Polynucleotide 5'-Hydroxyl-Kinase
An enzyme that catalyzes the transfer of a phosphate group to the 5'-terminal hydroxyl groups of DNA and RNA. EC 2.7.1.78.
Glucans
Polysaccharides composed of repeating glucose units. They can consist of branched or unbranched chains in any linkages.
Multienzyme Complexes
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.
Amino Acid Sequence
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Hydrolysis
The process of cleaving a chemical compound by the addition of a molecule of water.
Fermentation
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
Substrate Specificity
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
Cellulases
A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.
Enterocolitis, Pseudomembranous
An acute inflammation of the INTESTINAL MUCOSA that is characterized by the presence of pseudomembranes or plaques in the SMALL INTESTINE (pseudomembranous enteritis) and the LARGE INTESTINE (pseudomembranous colitis). It is commonly associated with antibiotic therapy and CLOSTRIDIUM DIFFICILE colonization.
Glucan 1,3-beta-Glucosidase
An exocellulase with specificity for 1,3-beta-D-glucasidic linkages. It catalyzes hydrolysis of beta-D-glucose units from the non-reducing ends of 1,3-beta-D-glucans, releasing GLUCOSE.
Genes, Bacterial
The functional hereditary units of BACTERIA.
Ethanol
A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.
Acetylesterase
An enzyme that catalyzes the conversion of acetate esters and water to alcohols and acetate. EC 3.1.1.6.
Clostridium acetobutylicum
A species of gram-positive bacteria in the family Clostridiaceae, used for the industrial production of SOLVENTS.
Cloning, Molecular
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
DNA, Bacterial
Deoxyribonucleic acid that makes up the genetic material of bacteria.
Base Sequence
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Sequence Homology, Amino Acid
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Organelles
Specific particles of membrane-bound organized living substances present in eukaryotic cells, such as the MITOCHONDRIA; the GOLGI APPARATUS; ENDOPLASMIC RETICULUM; LYSOSOMES; PLASTIDS; and VACUOLES.
Clostridium tetani
The cause of TETANUS in humans and domestic animals. It is a common inhabitant of human and horse intestines as well as soil. Two components make up its potent exotoxin activity, a neurotoxin and a hemolytic toxin.
Enzyme Stability
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Disaccharides
Oligosaccharides containing two monosaccharide units linked by a glycosidic bond.
Clostridium cellulolyticum
A species of gram-positive bacteria in the family Clostridiaceae. It is a cellulolytic, mesophilic species isolated from decayed GRASS.
Temperature
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
Crystallography, X-Ray
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Polysaccharides
Chromosomal Proteins, Non-Histone
Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.
Binding Sites
The parts of a macromolecule that directly participate in its specific combination with another molecule.
Botulinum Toxins
Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.
Protein Structure, Tertiary
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
Hot Temperature
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
Carbohydrate Metabolism
Cellular processes in biosynthesis (anabolism) and degradation (catabolism) of CARBOHYDRATES.
Clostridium sordellii
A species of gram-positive bacteria in the family Clostridiaceae, found in INTESTINES and SOIL.
Sequence Analysis, DNA
A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.
Electrophoresis, Polyacrylamide Gel
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Bacterial Toxins
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Membrane Proteins
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
Crystallization
The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Structural insights into the mechanism of formation of cellulosomes probed by small angle X-ray scattering. (1/130)

Exploring the mechanism by which the multiprotein complexes of cellulolytic organisms, the cellulosomes, attain their exceptional synergy is a challenge for biologists. We have studied the solution structures of the Clostridium cellulolyticum cellulosomal enzyme Cel48F in the free and complexed states with cohesins from Clostridium thermocellum and Clostridium cellulolyticum by small angle x-ray scattering in order to investigate the conformational events likely to occur upon complexation. The solution structure of the free cellulase indicates that the dockerin module is folded, whereas the linker connecting the catalytic module to the dockerin is extended and flexible. Remarkably, the docking of the different cohesins onto Cel48F leads to a pleating of the linker. The global structure determined here allowed modeling of the atomic structure of the C. cellulolyticum dockerin-cohesin interface, highlighting the local differences between both organisms responsible for the species specificity.  (+info)

Design and production in Aspergillus niger of a chimeric protein associating a fungal feruloyl esterase and a clostridial dockerin domain. (2/130)

A chimeric enzyme associating feruloyl esterase A (FAEA) from Aspergillus niger and dockerin from Clostridium thermocellum was produced in A. niger. A completely truncated form was produced when the dockerin domain was located downstream of the FAEA (FAEA-Doc), whereas no chimeric protein was produced when the bacterial dockerin domain was located upstream of the FAEA (Doc-FAEA). Northern blot analysis showed similar transcript levels for the two constructs, indicating a posttranscriptional bottleneck for Doc-FAEA production. The sequence encoding the first 514 amino acids from A. niger glucoamylase and a dibasic proteolytic processing site (kex-2) were fused upstream of the Doc-FAEA sequence. By using this fusion strategy, the esterase activity found in the extracellular medium was 20-fold-higher than that of the wild-type reference strain, and the production yield was estimated to be about 100 mg of chimeric protein/liter. Intracellular and extracellular production was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, dockerin-cohesin interaction assays, and Western blotting. Labeled cohesins detected an intact extracellular Doc-FAEA of about 43 kDa and a cleaved-off dockerin domain of about 8 kDa. In addition, an intracellular 120-kDa protein was recognized by using labeled cohesins and antibodies raised against FAEA. This protein corresponded to the unprocessed Doc-FAEA form fused to glucoamylase. In conclusion, these results indicated that translational fusion to glucoamylase improved the secretion efficiency of a chimeric Doc-FAEA protein and allowed production of the first functional fungal enzyme joined to a bacterial dockerin.  (+info)

Interactions between immunoglobulin-like and catalytic modules in Clostridium thermocellum cellulosomal cellobiohydrolase CbhA. (3/130)

Cellobiohydrolase CbhA from Clostridium thermocellum cellulosome is a multi-modular protein composed starting from the N-terminus of a carbohydrate-binding module (CBM) of family 4, an immunoglobulin(Ig)-like module, a catalytic module of family 9 glycoside hydrolases (GH9), X1(1) and X1(2) modules, a CBM of family 3 and a dockerin module. Deletion of the Ig-like module from the Ig-GH9 construct results in complete inactivation of the GH9 module. The crystal structure of the Ig-GH9 module pair reveals the existence of an extensive module interface composed of over 40 amino acid residues of both modules and maintained through a large number of hydrophilic and hydrophobic interactions. To investigate the importance of these interactions between the two modules, we compared the secondary and tertiary structures and thermostabilities of the individual Ig-like and GH9 modules and the Ig-GH9 module pair using both circular dichroism (CD) spectroscopy and differential scanning calorimetry (DSC). Thr230, Asp262 and Asp264 of the Ig-like module are located in the module interface of the Ig-GH9 module pair and are suggested to be important in 'communication' between the modules. These residues were mutated to alanyl residues. The structure, stability and catalytic properties of the native Ig-GH9 and its D264A and T230A/D262A mutants were compared. The results indicate that despite being able to fold relatively independently, the Ig-like and GH9 modules interact and these interactions affect the final fold and stability of each module. Mutations of one or two amino acid residues lead to destabilization and change of the mechanism of thermal unfolding of the polypeptides. The enzymatic properties of native Ig-GH9, D264A and T230A/D262A mutants are similar. The results indicate that inactivation of the GH9 module occurs as a result of multiple structural disturbances finally affecting the topology of the catalytic center.  (+info)

Regulation of cellulase synthesis in batch and continuous cultures of Clostridium thermocellum. (4/130)

Regulation of cell-specific cellulase synthesis (expressed in milligrams of cellulase per gram [dry weight] of cells) by Clostridium thermocellum was investigated using an enzyme-linked immunosorbent assay protocol based on antibody raised against a peptide sequence from the scaffoldin protein of the cellulosome (Zhang and Lynd, Anal. Chem. 75:219-227, 2003). The cellulase synthesis in Avicel-grown batch cultures was ninefold greater than that in cellobiose-grown batch cultures. In substrate-limited continuous cultures, however, the cellulase synthesis with Avicel-grown cultures was 1.3- to 2.4-fold greater than that in cellobiose-grown cultures, depending on the dilution rate. The differences between the cellulase yields observed during carbon-limited growth on cellulose and the cellulase yields observed during carbon-limited growth on cellobiose at the same dilution rate suggest that hydrolysis products other than cellobiose affect cellulase synthesis during growth on cellulose and/or that the presence of insoluble cellulose triggers an increase in cellulase synthesis. Continuous cellobiose-grown cultures maintained either at high dilution rates or with a high feed substrate concentration exhibited decreased cellulase synthesis; there was a large (sevenfold) decrease between 0 and 0.2 g of cellobiose per liter, and there was a much more gradual further decrease for cellobiose concentrations >0.2 g/liter. Several factors suggest that cellulase synthesis in C. thermocellum is regulated by catabolite repression. These factors include: (i) substantially higher cellulase yields observed during batch growth on Avicel than during batch growth on cellobiose, (ii) a strong negative correlation between the cellobiose concentration and the cellulase yield in continuous cultures with varied dilution rates at a constant feed substrate concentration and also with varied feed substrate concentrations at a constant dilution rate, and (iii) the presence of sequences corresponding to key elements of catabolite repression systems in the C. thermocellum genome.  (+info)

Functions of family-22 carbohydrate-binding module in Clostridium thermocellum Xyn10C. (5/130)

Clostridium thermocellum xylanase Xyn10C (formerly XynC) is a modular enzyme, comprising a family-22 carbohydrate-binding module (CBM), a family-10 catalytic module of the glycoside hydrolases, and a dockerin module responsible for cellulosome assembly consecutively from the N-terminus. To study the functions of the CBM, truncated derivatives of Xyn10C were constructed: a recombinant catalytic module polypeptide (rCM), a family-22 CBM polypeptide (rCBM), and a polypeptide composed of the family-22 CBM and CM (rCBM-CM). The recombinant proteins were characterized by enzyme and binding assays. Although the catalytic activity of rCBM-CM toward insoluble xylan was four times higher than that of rCM toward the same substrate, removal of the CBM did not severely affect catalytic activity toward soluble xylan or beta-1,3-1,4-glucan. rCBM showed an affinity for amorphous celluloses and insoluble and soluble xylan in qualitative binding assays. The optimum temperature of rCBM-CM was 80 degrees C and that of rCM was 60 degrees C. These results indicate that the family-22 CBM of C. thermocellum Xyn10C not only was responsible for the binding of the enzyme to the substrates, but also contributes to the stability of the CM in the presence of the substrate at high temperatures.  (+info)

Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin. (6/130)

In recent work, we reported the self-assembly of a comprehensive set of defined "bifunctional" chimeric cellulosomes. Each complex contained the following: (i) a chimeric scaffoldin possessing a cellulose-binding module and two cohesins of divergent specificity and (ii) two cellulases, each bearing a dockerin complementary to one of the divergent cohesins. This approach allowed the controlled integration of desired enzymes into a multiprotein complex of predetermined stoichiometry and topology. The observed enhanced synergy on recalcitrant substrates by the bifunctional designer cellulosomes was ascribed to two major factors: substrate targeting and proximity of the two catalytic components. In the present work, the capacity of the previously described chimeric cellulosomes was amplified by developing a third divergent cohesin-dockerin device. The resultant trifunctional designer cellulosomes were assayed on homogeneous and complex substrates (microcrystalline cellulose and straw, respectively) and found to be considerably more active than the corresponding free enzyme or bifunctional systems. The results indicate that the synergy between two prominent cellulosomal enzymes (from the family-48 and -9 glycoside hydrolases) plays a crucial role during the degradation of cellulose by cellulosomes and that one dominant family-48 processive endoglucanase per complex is sufficient to achieve optimal levels of synergistic activity. Furthermore cooperation within a cellulosome chimera between cellulases and a hemicellulase from different microorganisms was achieved, leading to a trifunctional complex with enhanced activity on a complex substrate.  (+info)

Cellulase, clostridia, and ethanol. (7/130)

Biomass conversion to ethanol as a liquid fuel by the thermophilic and anaerobic clostridia offers a potential partial solution to the problem of the world's dependence on petroleum for energy. Coculture of a cellulolytic strain and a saccharolytic strain of Clostridium on agricultural resources, as well as on urban and industrial cellulosic wastes, is a promising approach to an alternate energy source from an economic viewpoint. This review discusses the need for such a process, the cellulases of clostridia, their presence in extracellular complexes or organelles (the cellulosomes), the binding of the cellulosomes to cellulose and to the cell surface, cellulase genetics, regulation of their synthesis, cocultures, ethanol tolerance, and metabolic pathway engineering for maximizing ethanol yield.  (+info)

Regulation of major cellulosomal endoglucanases of Clostridium thermocellum differs from that of a prominent cellulosomal xylanase. (8/130)

The expression of scaffoldin-anchoring genes and one of the major processive endoglucanases (CelS) from the cellulosome of Clostridium thermocellum has been shown to be dependent on the growth rate. For the present work, we studied the gene regulation of selected cellulosomal endoglucanases and a major xylanase in order to examine the previously observed substrate-linked alterations in cellulosome composition. For this purpose, the transcript levels of genes encoding endoglucanases CelB, CelG, and CelD and the family 10 xylanase XynC were determined in batch cultures, grown on either cellobiose or cellulose, and in carbon-limited continuous cultures at different dilution rates. Under all conditions tested, the transcript levels of celB and celG were at least 10-fold higher than that of celD. Like the major processive endoglucanase CelS, the transcript levels of these endoglucanase genes were also dependent on the growth rate. Thus, at a rate of 0.04 h(-1), the levels of celB, celG, and celD were threefold higher than those obtained in cultures grown at maximal rates (0.35 h(-1)) on cellobiose. In contrast, no clear correlation was observed between the transcript level of xynC and the growth rate-the levels remained relatively high, fluctuating between 30 and 50 transcripts per cell. The results suggest that the regulation of C. thermocellum endoglucanases is similar to that of the processive endoglucanase celS but differs from that of a major cellulosomal xylanase in that expression of the latter enzyme is independent of the growth rate.  (+info)

Clostridium thermocellum CelJ protein Summary Report | CureHunterClostridium thermocellum CelJ protein Summary Report | CureHunter
Clostridium thermocellum CelJ protein: isolated from Clostridium thermocellum; amino acid sequence in first source; GenBank D83704
http://www.curehunter.com/public/keywordSummaryC101903-Clostridium-thermocellum-CelJ-protein.do
Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production | BMC Systems Biology | Full TextGenome-scale metabolic analysis of Clostridium thermocellum for bioethanol production | BMC Systems Biology | Full Text
Current and future demands for renewable energy sources have spurred research in developing biofuels. One promising route for biofuel production is to use an organism-based bioprocess where cellulose could be converted to biofuel. One of the main challenges to this approach is that there are relatively few cellulolytic organisms capable of biofuel production, and none of these are especially well-characterized at present. Here we have implemented a computational modeling approach to study C. thermocellum, an anaerobic thermophile with high biofuel production potential. In this study, the development of a genome-scale metabolic model of C. thermocellum was used to provide a framework for analyzing the basic metabolic functions of C. thermocellum and improving its ethanol production capabilities. Overall, we report the construction of a genome-scale metabolic model of C. thermocellum, i SR432, and the accuracy of this model to predict cellular phenotypes (growth and fermentation product secretion) ...
https://bmcsystbiol.biomedcentral.com/articles/10.1186/1752-0509-4-31
Recombinant Clostridium Thermocellum ispE Protein (aa 1-283) - Creative BiolabsRecombinant Clostridium Thermocellum ispE Protein (aa 1-283) - Creative Biolabs
Creative Biolabs offers the best Recombinant Clostridium Thermocellum ispE Protein (aa 1-283), which is useful for vaccine development.
https://www.creative-biolabs.com/vaccine/recombinant-clostridium-thermocellum-ispe-protein-aa-1-283-21506.htm
Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum | PNASMutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum | PNAS
NAD or NADP differ with respect to presence or absence of a phosphate group esterified at the 2′ position of the adenosine ribose and are similar at the level of structure. Rosell et al. (21) have shown complete reversal of ADH cofactor specificity in crystallography studies. However, NAD is typically used in oxidative, ATP-generating degradation reactions, and NADP usually acts as a reductant in reductive biosynthetic reactions. An ethanol cycle with two ADH isozymes catalyzing opposite reactions (i.e., ethanol oxidation or ethanol synthesis) has been proposed for Thermoanaerobacter pseudoethanolicus (formerly Clostridium thermohydrosulfuricum) (22) and similarly for the naturally more ethanol-tolerant Zymomonas mobilis (23). We did not test the ethanol cycling hypothesis in this study, but net ethanol oxidation did not appear to be a major detoxification mechanism (SI Appendix, Fig. S6).. The reduction in specific activity with respect to NADH was far greater (∼25-fold less activity) than ...
https://www.pnas.org/content/108/33/13752
Genetic engineering of Clostridium thermocellum DSM1313 for enhanced ethanol production | BMC Biotechnology | Full TextGenetic engineering of Clostridium thermocellum DSM1313 for enhanced ethanol production | BMC Biotechnology | Full Text
Blending of ethanol with fossil fuel is recommended to address the problem of increasing demand for transportation fuel without environmental pollution. This implementation was planned because ethanol is derived from renewable biological sources, and has promising properties such as anti-knock potential [1, 2] and cleaner combustion [3, 4]. Several countries around the world are adopting this strategy, and are planning to increase the percentage of ethanol blending in a phased manner [5]. In this context, allocation of natural resources for ethanol production in place of food production, and completing interests in ethanol for industrial and potable purposes are the major concerns to be addressed. For example, sugarcane molasses serves as the sole feedstock for bioethanol production in India where the annual production is approximately 2.7 billion litres of which only 30 % is offered for fuel purposes [6, 7]. When considering the supply of feedstock, increasing the production of ethanol from ...
https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-016-0260-2
RCSB PDB - 1GKL: S954A mutant of the feruloyl esterase module from clostridium thermocellum complexed with ferulic...RCSB PDB - 1GKL: S954A mutant of the feruloyl esterase module from clostridium thermocellum complexed with ferulic...
1GKL: The Structure of the Feruloyl Esterase Module of Xylanase 10B from Clostridium Thermocellum Provides Insights Into Substrate Recognition
http://www.rcsb.org/pdb/explore/litView.do?structureId=1GKL
RCSB PDB - 1GKK: Feruloyl esterase domain of XynY from clostridium thermocellumRCSB PDB - 1GKK: Feruloyl esterase domain of XynY from clostridium thermocellum
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
https://www.rcsb.org/structure/1GKK
CEA/DSV/IBeB/SBVMe/LEMIRE - P2CS Database Clostridium thermocellum DSM 1313
Signal transduction proteins, Histidine Kinase, Response regulator, Phophotransfer protein, HPT, Phosphorelay, complete metagenomes browser, TCS, Prokaryotic Two-Component Systems database, P2CS, annotation browser, MIST, SENTRA
http://www.p2cs.org/page.php?base=Clot3DB§ion=genelist&type=misChr
Aphios Announces Grant of Cellulosic Biomass Patent - CleanTechies
technology for cellulosic biomass conversion, recently put out a press release announcing the grant of U.S. Patent No. 8,540,847 (847 Patent).. Entitled Methods and apparatus for processing cellulosic biomass, the 847 Patent is directed to methods and apparatus for making ethanol or other biofuels using what Aphios calls its Aosic process.. The apparatus (11) described and claimed in the patent comprises a first vessel (13) for receiving cellulosic biomass and conveying means (15) in fluid communication with the first vessel (13). The apparatus (11) also comprises supercritical, critical, or near critical fluid means (17), which includes a source of gas, such as gas tank (41), holding carbon dioxide pressurized to form supercritical, critical, or near critical fluid.. The fluid means (17) is in fluid communication with conveying means (15) via conduit (31). A pump (47) is connected to a heat exchanger (55), which controls the temperature of the supercritical, critical, or near critical ...
https://cleantechies.com/2014/02/19/aphios-announces-grant-of-cellulosic-biomass-patent/
Exploring complex cellular phenotypes and model-guided strain design w by R. Adam Thompson, Sanjeev Dahal et al.Exploring complex cellular phenotypes and model-guided strain design w by R. Adam Thompson, Sanjeev Dahal et al.
Background: Clostridium thermocellum is a gram-positive thermophile that can directly convert lignocellulosic material into biofuels. The metabolism of C. thermocellum contains many branches and redundancies which limit biofuel production, and typical genetic techniques are time-consuming. Further, the genome sequence of a genetically tractable strain C. thermocellum DSM 1313 has been recently sequenced and annotated. Therefore, developing a comprehensive, predictive, genome-scale metabolic model of DSM 1313 is desired for elucidating its complex phenotypes and facilitating model-guided metabolic engineering. Results: We constructed a genome-scale metabolic model iAT601 for DSM 1313 using the KEGG database as a scaffold and an extensive literature review and bioinformatic analysis for model refinement. Next, we used several sets of experimental data to train the model, e.g., estimation of the ATP requirement for growth-associated maintenance (13.5 mmol ATP/g DCW/h) and cellulosome synthesis (57 mmol ATP
http://trace.tennessee.edu/utk_chembiopubs/99/
ACVX00000000.1 Ruminiclostridium thermocellum DSM 2360 :: NCBIACVX00000000.1 Ruminiclostridium thermocellum DSM 2360 :: NCBI
The Ruminiclostridium thermocellum DSM 2360 whole genome shotgun (WGS) project has the project accession ACVX00000000. This version of the project (01) has the accession number ACVX01000000, and consists of sequences ACVX01000001-ACVX01000110.. URL -- http://www.jgi.doe.gov JGI Project ID: 4085028 Source DNA and Bacteria available from Christopher L. Hemme ([email protected]) Contacts: Christopher L. Hemme ([email protected]) David Bruce ([email protected]) Whole genome sequencing and draft assembly at JGI-PGF Annotation by JGI-ORNL The JGI and collaborators endorse the principles for the distribution and use of large scale sequencing data adopted by the larger genome sequencing community and urge users of this data to follow them. It is our intention to publish the work of this project in a timely fashion and we welcome collaborative interaction on the project and analysis. (http://www.genome.gov/page.cfm?pageID=10506376).. ...
https://www.ncbi.nlm.nih.gov/Traces/wgs/?val=ACVX01
KEGG ENZYME: 3.2.1.176
Some exocellulases, most of which belong to the glycoside hydrolase family 48 (GH48, formerly known as cellulase family L), act at the reducing ends of cellulose and similar substrates. The CelS enzyme from Clostridium thermocellum is the most abundant subunit of the cellulosome formed by the organism. It liberates cellobiose units from the reducing end by hydrolysis of the glycosidic bond, employing an inverting reaction mechanism [2]. Different from EC 3.2.1.91, which attacks cellulose from the non-reducing end ...
http://www.genome.jp/dbget-bin/www_bget?ec:3.2.1.176
May | 2018 | Peptide Solubility
thermocellum and C. josui scaffolding proteins in this study. The C. thermocellum strain F1 was used for the isolation of genomic DNA (Sakka et al., 1989). Escherichia coli strains XL1-Blue and BL21(DE3) RIL (Novagen) were used for the cloning and expression of parts of the C. thermocellum http://www.selleckchem.com/products/NVP-AUY922.html xyn10C and xyn11A genes (DDBJ accession nos. D84188 and AB010958, respectively). Recombinant E. coli strains were cultured in Luria-Bertani (LB) broth supplemented with ampicillin (50 μg mL−1) or kanamycin (50 μg mL−1) and chloramphenicol. (25 μg mL−1) at 37 °C. The DNA region encoding the native Xyn11A dockerin was amplified by PCR from the plasmid pKS101-1 (Hayashi et al., 1999) using the primers XynADF and XynADR (Table 1), digested with EcoRI and SalI and inserted into the EcoRI and SalI sites of pBluescipt II KS(+). After checking its nucleotide sequence, the inserted DNA fragment was cleaved from the recombinant plasmid using EcoRI and SalI, ...
https://peptidesolubility.com/2018/05
CAZy - GH141
Created after Ndeh et al., Nature (2017) 544:65-70 (PMID=28329766) who have shown the a-L-fucosidase activity of the B. thetaiotaomicron enzyme. The xylanase activity of the (Rumini)clostridium thermocellum enzyme was shown by Heinze et al. (PMID=28894250 ...
http://www.cazy.org/GH141.html
Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile...Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile...
A major challenge to converting lignocellulose into fuels and chemicals is the lack of microbial access to the complex polysaccharides that comprise the plant cell wall. C. bescii is unique in its ability to degrade and coutilize sugars from both hemicellulose (C5) and cellulose (C6). Thus, for this extremely thermophilic bacterium, lignin is the major barrier to complete plant biomass conversion.. The transgenic switchgrass lines MYB Trans and COMT3(+), their respective parental lines (cv. Alamo), and a naturally occurring, low-recalcitrance switchgrass (cv. CR) were all solubilized by C. bescii but to different extents. Significant variations in solubilization were seen between the different parental lines (WT); this validates the observations previously reported (50) using CBP with Clostridium thermocellum. While the C. bescii growth rate and carbohydrate solubilization were higher on the transgenic lines than on their parental line, both MYB lines were especially resistant to microbial ...
https://aem.asm.org/content/83/17/e00969-17?ijkey=079638bf3e892b230757688b7b3a897e0904ed1d&keytype2=tf_ipsecsha
Characterization ofClostridium thermocellumstrains with disrupted fermentation end-product pathways | SpringerLinkCharacterization ofClostridium thermocellumstrains with disrupted fermentation end-product pathways | SpringerLink
Clostridium thermocellum is a thermophilic, cellulolytic anaerobe that is a candidate microorganism for industrial biofuels production. Strains with mutations in genes associated with production of l-
https://link.springer.com/article/10.1007%2Fs10295-013-1275-5
Ethanol from Cellulosic Biomass
One of the barriers to the production of ethanol from cellulosic biomass is the toughness of the cellulosic structure, and its resistance to chemical and enzymatic hydrolysis and insolubility in
http://www.alternative-energy-news.info/ethanol-cellulose-biomass/
cbhA - Glucanase - Hungateiclostridium thermocellum - cbhA gene & proteincbhA - Glucanase - Hungateiclostridium thermocellum - cbhA gene & protein
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
https://www.uniprot.org/uniprot/Q59325
Difference between CIP & CIPADifference between CIP & CIPA
The difference between CIP & CIPA is that in CIP the person only cant feel pain but in case of CIPA the person cannot sweat and probably has malformations in his/ her body. ...
http://raregeneticdisorder.blogspot.com/2012/07/difference-between-cip-cipa.html
Elimination of hydrogenase active site assembly blocks H 2 production and increases ethanol yield in Clostridium thermocellum |...Elimination of hydrogenase active site assembly blocks H 2 production and increases ethanol yield in Clostridium thermocellum |...
The native ability of Clostridium thermocellum to rapidly consume cellulose and produce ethanol makes it a leading candidate for a consolidated bioprocessing (CBP) biofuel production strategy. C. thermocellum also synthesizes lactate, formate, acetate, H2, and amino acids that compete with ethanol production for carbon and electrons. Elimination of H2 production could redirect carbon flux towards ethanol production by making more electrons available for acetyl coenzyme A reduction to ethanol. H2 production in C. thermocellum is encoded by four hydrogenases. Rather than delete each individually, we targeted hydrogenase maturase gene hydG, involved in converting the three [FeFe] hydrogenase apoenzymes into holoenzymes. Further deletion of the [NiFe] hydrogenase (ech) resulted in a mutant that functionally lacks all four hydrogenases. H2 production in ∆hydG∆ech was undetectable, and the ethanol yield nearly doubled to 64% of the maximum theoretical yield. Genomic analysis of ∆hydG revealed a mutation
https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-015-0204-4
Deletion of the Cel48S cellulase from Clostridium thermocellum | PNASDeletion of the Cel48S cellulase from Clostridium thermocellum | PNAS
Deletion of Cel48S from C. thermocellum led to a decrease in the enzymatic hydrolysis rate, a decrease in microbial hydrolysis rate, and a decrease in biomass formation during growth on Avicel.. The similarity of enzyme saturation curves for the WT and parent strains suggests that the ΔpyrF mutation in the parent strain has no effect on cellulosome function, as expected. The S mutant strain, however, exhibited a reduction in both specific activity and saturation rate. A reduction in specific activity is indicative of impaired function and consistent with decreased synergy among components of the cellulosome in the absence of Cel48S (3).. The role of GH families in cellulose solubilization is a topic of much debate. Family 48 cellulases are a prominent component of many bacterial cellulase systems and, due to their ubiquity, are thought to play an important role in cellulose solubilization (21). On one hand, disruption of the single family 9 GH in C. phytofermentans eliminated its ability to ...
https://www.pnas.org/content/107/41/17727?ijkey=fc2d41b731e81805e4616de3833aff59989a40b5&keytype2=tf_ipsecsha
β-Mannanase 26C from Clostridium thermocellum, Recombinant(EC 3.2.1.78) - Creative Enzymesβ-Mannanase 26C from Clostridium thermocellum, Recombinant(EC 3.2.1.78) - Creative Enzymes
Mannan endo-1,4-beta-mannosidase is an enzyme with system name 4-beta-D-mannan mannanohydrolase. This enzyme catalyses the following chemical reaction: Random hydrolysis
https://www.creative-enzymes.com/product/-Mannanase-26C-From-Clostridium-Thermocellum-Recombinant_3662.html
PNKP gene: MedlinePlus GeneticsPNKP gene: MedlinePlus Genetics
The PNKP gene provides instructions for making the polynucleotide kinase-phosphatase (PNKP) enzyme. Learn about this gene and related health conditions.
https://medlineplus.gov/genetics/gene/pnkp/
Reassembly and co-crystallization of a family 9 processive endoglucanase from its component parts: Structural and functional...Reassembly and co-crystallization of a family 9 processive endoglucanase from its component parts: Structural and functional...
Non-cellulosomal processive endoglucanase 9I (Cel9I) from Clostridium thermocellum is a modular protein, consisting of a family-9 glycoside hydrolase (GH9) catalytic module and two family-3 carbohydrate-binding modules (CBM3c and CBM3b), separated by linker regions. GH9 does not show cellulase activity when expressed without CBM3c and CBM3b and the presence of the CBM3c was previously shown to be essential for endoglucanase activity. Physical reassociation of independently expressed GH9 and CBM3c modules (containing linker sequences) restored 60-70% of the intact Cel9I endocellulase activity. However, the mechanism responsible for recovery of activity remained unclear. In this work we independently expressed recombinant GH9 and CBM3c with and without their interconnecting linker in Escherichia coli. We crystallized and determined the molecular structure of the GH9/linker-CBM3c heterodimer at a resolution of 1.68 Å to understand the functional and structural importance of the mutual spatial orientation
https://peerj.com/preprints/1133/
Metabolic control ofClostridium thermocellumvia inhibition of hydrogenase activity and the glucose transport rate | SpringerLinkMetabolic control ofClostridium thermocellumvia inhibition of hydrogenase activity and the glucose transport rate | SpringerLink
Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon dioxide, and hydrogen gas (H2) are also produced. The effect of hydrogenase
https://link.springer.com/article/10.1007%2Fs00253-011-3812-3
New Discovery Could Improve Ethanol Production - CleanTechies
Researchers at the Department of Energys (DOE) BioEnergy Science Center (BESC) have made a discovery that could increase the production of ethanol and lower its costs.. They say they have pinpointed the gene that controls ethanol production capacity in a microorganism, which could be the missing link in developing more efficient and cheaper biomass crops.. Current methods to make ethanol from a type of biomass found in switchgrass and agricultural waste require the addition of expensive enzymes to break down the plants barriers that guard energy-rich sugars. The gene discovered controls ethanol production in a microorganism known as Clostridium thermocellum. With it scientists will be able to experiment with genetically altering biomass plants to produce more ethanol.. The Department of Energy relies on the scientific discoveries of its labs and research centers to improve the production of clean energy sources, said Energy Secretary Steven Chu. This discovery is an important step in ...
http://cleantechies.com/2011/08/15/new-discovery-could-improve-ethanol-production/
Complete Genome Sequence of Clostridium clariflavum DSM 19732 | Environmental Microbiome | Full TextComplete Genome Sequence of Clostridium clariflavum DSM 19732 | Environmental Microbiome | Full Text
Clostridium clariflavum is a Cluster III Clostridium within the family Clostridiaceae isolated from thermophilic anaerobic sludge (Shiratori et al, 2009). This species is of interest because of its similarity to the model cellulolytic organism Clostridium thermocellum and for the ability of environmental isolates to break down cellulose and hemicellulose. Here we describe features of the 4,897,678 bp long genome and its annotation, consisting of 4,131 protein-coding and 98 RNA genes, for the type strain DSM 19732.
https://environmentalmicrobiome.biomedcentral.com/articles/10.4056/sigs.2535732/tables/1
Single, key gene discovery could streamline production of... ( WASHINGTON DC -- A team of researche...)Single, key gene discovery could streamline production of... ( WASHINGTON DC -- A team of researche...)
...WASHINGTON DC -- A team of researchers at the Department of Energys ... The Department of Energy relies on the scientific discoveries of its ...The discovery of the gene controlling ethanol production in a microorg...Although scientists have studied Clostridium thermocellum for decades...,Single,,key,gene,discovery,could,streamline,production,of,biofuels,,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
http://www.bio-medicine.org/biology-news-1/Single--key-gene-discovery-could-streamline-production-of-biofuels--20819-1/
Dr Ellis ONeill | Department of Plant Sciences
21. Kelly, S., Ivens, A., Mott,G. A., ONeill, E. C., Emms, D., Macleod, O., Voorheis, P., Tyler, K., Clark, M., Matthews, J., Matthews, K., Carrington, M. C. An Alternative Strategy for Trypanosome Survival in the Mammalian Bloodstream Revealed through Genome and Transcriptome Analysis of the Ubiquitous Bovine Parasite Trypanosoma (Megatrypanum) theileri. Genome Biology and Evolution (2017) 9, 2093-2109 DOI: 10.1093/gbe/evx152. 20. ONeill, E. C., Pergolizzi, G., Stevenson, C. E. M., Lawson, D. M., Nepogodiev, S. A., and Field, R. A. Cellodextrin phosphorylase from Clostridium thermocellum: X-ray crystal structure and substrate specificity analysis. Carbohydrate Research (2017) DOI: 10.1016/j.carres.2017.07.005. 19. ONeill, E. C. and Kelly, S. Engineering biosynthesis of high-value compounds in photosynthetic organisms. Critical Reviews in Biotechnology (2017) 37, 779-802 DOI: 10.1080/07388551.2016.1237467. 18. ONeill, E. C. Biomolecular engineering of micro-organisms for natural products ...
http://www.plants.ox.ac.uk/node/1558
Browsing Titles index Beyond hairballs: depicting complexity of a kinase-phosphatase network in the budding yeastBrowsing Titles index Beyond hairballs: depicting complexity of a kinase-phosphatase network in the budding yeast
Les kinases et les phosphatases (KP) représentent la plus grande famille des enzymes dans la cellule. Elles régulent les unes les autres ainsi que 60 % du protéome, formant des réseaux complexes kinase-phosphatase (KP-Net) jouant un rôle essentiel dans ...
https://papyrus.bib.umontreal.ca/xmlui/handle/1866/2591/browse?type=titleindex&value=Beyond+hairballs%3A+depicting+complexity+of+a+kinase-phosphatase+network+in+the+budding+yeast
Researchers report a novel approach to cellulose hydrolysis | Biomassmagazine.com
A novel approach to cellulose hydrolysis using a consortium of motile bacteria moving on solid surfaces and carrying microbial luggage, another bacteria that can efficiently hydrolyze cellulose, was demonstrated by the group of researchers.
http://biomassmagazine.com/articles/12268/researchers-report-a-novel-approach-to-cellulose-hydrolysis
Necessity of enzymatic hydrolysis for production and functionalization of nanocellulosesNecessity of enzymatic hydrolysis for production and functionalization of nanocelluloses
Nanocellulose (NC) from cellulosic biomass has recently gained attention owing to their biodegradable nature, low density, high mechanical properties, economic value and renewability. They still suffer, however, some drawbacks. The challenges are the exploration of raw materials, scaling, recovery of chemicals utilized for the production or functionalization and most important is toxic behavior that hinders them from implementing in medical/pharmaceutical field. This review emphasizes the structural behavior of cellulosic biomass and biological barriers for enzyme interactions, which are pertinent to understand the enzymatic hydrolysis of cellulose for the production of NCs. Additionally, the enzymatic catalysis for the modification of solid and NC is discussed. The utility of various classes of enzymes for introducing desired functional groups on the surface of NC has been further examined. Thereafter, a green mechanistic approach is applied for understanding at molecular level. ...
http://ltu.diva-portal.org/smash/record.jsf?pid=diva2:1076811
KEGG SSDB Paralog Search Result: cthe:Chro 3546KEGG SSDB Paralog Search Result: cthe:Chro 3546
T02360 (aalt,achr,acta,actc,amyb,amyc,asw,cmos,cthd,cyl,dfn,ehl,fek,fva,hta,kak,kmx,kpnk,lei,lfb,lsh,lys,mcol,msub,mtab,noe,oor,paru,phs,pje,png,ptd,rpln,sclo,scou,seny,sera,sfz,slb,slw,snl,sphc,sphy,srub,taj : calculation not yet completed ...
http://www.kegg.jp/ssdb-bin/ssdb_paralog?org_gene=cthe:Chro_3546
Pre GI: BLASTP Hits
General Information: Country: Japan; Isolation: Methanogenic sludge; Temp: Thermophile; Temp: 55C. Clostridium clariflavum is a thermophilic cellulose degrader closely related to C. thermocellum, with equivalent cellulolytic activity. ...
http://pregi.bi.up.ac.za/pre_gi_blast.php?blast_source_accession=NC_016627&blast_cds_query=NC_016627:2799453:2830539&sort=cds
CAZy - GH138
Created after Ndeh et al., Nature (2017) 544(7648):65-70 (PMID=28329766); mechanism and structure after Labourel et al., JBC (2019) (PMID=30877196 ...
http://www.cazy.org/GH138.html
Cellulosic biomass could help meet Californias transportation fuel needsCellulosic biomass could help meet Californias transportation fuel needs
Author(s): Wyman, Charles E.; Yang, Bin | Abstract: Cellulosic biomass, which includes agricultural and forestry residues and woody and herbaceous plants, is the only low-cost resource that can support the sustainable production of liquid fuels on a large enough scale to significantly address our transportation energy needs. The biological conversion of cellulosic biomass to ethanol could offer high yields at low costs, but only if we can improve the technology for releasing simple sugars from recalcitrant biomass. We review key aspects of cellulosic ethanol production, including pretreatment and enzymatic hydrolysis technologies that present the greatest opportunities to lower processing costs. Although several companies seek to introduce cellulosic ethanol commercially, innovative measures are needed to help overcome the perceived risks of first applications.
https://escholarship.org/uc/item/4m52w8p8
NCHU Institutional Repository CRIS: 應用Bacillus subtilis WB800N生產人造纖維素體酵素與其固定化酵素之特性與動力學研究
1] P.R.V. Hamann, D.L. Serpa, A.S. Barreto da Cunha, B.R. de Camargo, K.O. Osiro, M. Valle de Sousa, C.R. Felix, R.N.G. Miller, E.F. Noronha, Evaluation of plant cell wall degrading enzyme production by Clostridium thermocellum B8 in the presence of raw agricultural wastes, Int. Biodeterior. Biodegrad., 105 (2015) 97-105. [2] J.M. Xiaorong Wu, Ron Madl, Donghai Wang, Biofuels from Lignocellulosic Biomass, Sustain. Biotechnol. , (2010) 19-41. [3] R.H. Doi, A. Kosugi, Cellulosomes: Plant-cell-wall-degrading enzyme complexes, Nat. Rev. Microbiol., 2 (2004) 541-551. [4] L.R. Lynd, W.H. van Zyl, J.E. McBride, M. Laser, Consolidated bioprocessing of cellulosic biomass: an update, Curr. Opin. Biotechnol., 16 (2005) 577-583. [5] J. Zaldivar, J. Nielsen, L. Olsson, Fuel ethanol production from lignocellulose: a challenge for metabolic engineering and process integration, Appl. Microbiol. Biotechnol., 56 (2001) 17-34. [6] B.S. Dien, M.A. Cotta, T.W. Jeffries, Bacteria engineered for fuel ethanol ...
http://ir.lib.nchu.edu.tw/handle/11455/99570
Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a...Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a...
The inherent recalcitrance of lignocellulosic biomass is one of the major economic hurdles for the production of fuels and chemicals from biomass. Additionally, lignin is recognized as having a negative impact on enzymatic hydrolysis of biomass, and as a result much interest has been placed on modifying the lignin pathway to improve bioconversion of lignocellulosic feedstocks. Down-regulation of the caffeic acid 3-O-methyltransferase (COMT) gene in the lignin pathway yielded switchgrass (Panicum virgatum) that was more susceptible to bioconversion after dilute acid pretreatment. Here we examined the response of these plant lines to milder pretreatment conditions with yeast-based simultaneous saccharification and fermentation and a consolidated bioprocessing approach using Clostridium thermocellum, Caldicellulosiruptor bescii and Caldicellulosiruptor obsidiansis. Unlike the S. cerevisiae SSF conversions, fermentations of pretreated transgenic switchgrass with C. thermocellum showed an apparent inhibition
https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/1754-6834-5-81
Superiority of the PCR-based Approach for Cloning the Acetate Kinase G by G. Ozcengiz, J-H Kim et al.Superiority of the PCR-based Approach for Cloning the Acetate Kinase G by G. Ozcengiz, J-H Kim et al.
Cloning of Clostridium thermocellum acetate kinase (ack) and/or phosphotransacetylase (pta) genes in Escherichia coli by functional complementation of ack and/or pta mutants was complicated by an alternative acetate assimilation pathway involving acetyl-CoA synthetase (ACS). In addition to the problems encountered with the complementation approach, cloning of these genes was not readily achieved using heterologous probing with corresponding genes from Escherichia coli and Methanosarcina thermophila due to the lack of sufficient homology. The use of a PCR-based approach, on the other hand, yielded a specific C. Thermocellum gene fragment which showed significant sequence identity to the ack gene for which primers were designed. The subcloned ack fragment was then successfully used as a probe for the isolation of the corresponding gene and restriction analysis of that region.
http://scholarsmine.mst.edu/biosci_facwork/10/
The Energy Blog: Worlds First Cellulosic Ethanol Plant
Adapted from Celunol launches commercial-scale cellulosic ethanol plant in Japan by C. Scott Miller, BIOconversion Blog, Jan. 21, 2007 BioEthanol Japan, on January 16, became the worlds first company to produce cellulosic ethanol from wood construction waste on a commercial basis. The plant in Osaka Prefecture has an annual capacity of 1.4 million liters (about 370,000 US gallons). In 2008, it plans to boost production to 4 million liters (1 million US gallons). BioEthanol Japan was established in 2004 by five companies, including construction firm Taisei Corp., trading house Marubeni Corp., Daiei Inter Nature System, and beermaker Sapporo Breweries Ltd. Marubeni is supplying the process technology, which it has licensed from US-based Celunol, to BioEthanol Japan Celunols technology is based on the metabolic engineering of microorganisms, a set of genetically engineered strains of Escherichia coli bacteria that can ferment both C6 (hexose) and C5 (pentose) sugars present in cellulosic biomass into
https://thefraserdomain.typepad.com/energy/2007/02/worlds_first_ce.html
Cellulosic Ethanol Start Up Reports Growth | EnergyCellulosic Ethanol Start Up Reports Growth | Energy
Massachusetts-based Verenium Corporation reports that total revenues for the quarter ended March 31, 2008 were $15.2 million compared to $11.3 million for first quarter of 2007. Verenium CEO Carlos Riva says he is encouraged by the overall results and achievements of the first quarter. As planned, our demonstration-scale cellulosic ethanol facility entered the start-up phase and is proceeding on schedule despite significant cost escalation and labor shortages being experienced across the energy sector, said Riva in a statement. We are at a transformational time for both the next-generation biofuels industry and Verenium, as the world is now actively seeking alternate sources of ethanol, particularly those derived from cellulosic biomass, added Riva. Verenium operates an R&D cellulosic ethanol pilot plants in Jennings, Louisiana and has entered the start-up phase at its 1.4 million gallon per year demonstration-scale facility. In addition, the companys process technology has been ...
https://energy.agwired.com/2008/05/05/cellulosic-ethanol-start-up-reports-growth/
Iowa project proves viability of cellulosic ethanol | Farm Industry NewsIowa project proves viability of cellulosic ethanol | Farm Industry News
Cellulosic ethanol is a 50-state solution. It can be produced in every single state in the nation. We have enough cellulosic biomass in this country that, with grain ethanol, we can produce enough clean, renewable ethanol to displace every gallon of foreign oil we currently import. The Project Liberty announcement by POET is the single-most important step forward for proving to all the naysayers that we are at the tip of having commercially viable cellulosic ethanol, said Tom Buis, CEO of Growth Energy ...
http://www.farmindustrynews.com/ethanol/iowa-project-proves-viability-cellulosic-ethanol?intlink=rceoc
Permalien vers The crystal structure of a family 5 endoglucanase mutant in complexed and uncomplexed forms reveals an induced...Permalien vers The crystal structure of a family 5 endoglucanase mutant in complexed and uncomplexed forms reveals an induced...
The structures of the Glu140--|Gln mutant of the Clostridium thermocellum endoglucanase CelC in unliganded form (CelC(E140Q)) and in complex with
https://research.pasteur.fr/fr/publication/the-crystal-structure-of-a-family-5-endoglucanase-mutant-in-complexed-and-uncomplexed-forms-reveals-an-induced-fit-activation-mechanism/
Structural Biochemistry/Cell Organelles/Plant Cell/Cellulose - Wikibooks, open books for an open worldStructural Biochemistry/Cell Organelles/Plant Cell/Cellulose - Wikibooks, open books for an open world
Cellulosomes known as the proficient nanomachine in nature are cell bound multi-enzyme complexes that break down cellulose and hemicelluloses. They are very important in the process of carbon turnover. The cellulosome complexes require highly ordered proteins (the interactions between cohesions and dockerins) to assembly cellulases and hemicellulases into a scaffold structure. The protein interactions between cohesion and dockerin play an important role in cellulosome assembly and the attachment of cellulosome to the surface of cells while remaining flexible to provide a stable catalytic synergy.. One function of cellulosomes is breaking down plants structural polysaccharides. It is hypothesize that the constraints of the cellulosomes system created by bacteria and fungi caused the deconstruction of plant cell wall to become more and more efficient. The splicing of the plant-cell wall involves the addition of enzymes on to a macromolecule complex will increase the effectiveness of the ...
https://en.wikibooks.org/wiki/Structural_Biochemistry/Cell_Organelles/Plant_Cell/Cellulose
GM Enters Strategic Relationship with Mascoma for Cellulosic Ethanol - Green Car Congress
Mascoma is developing a Consolidated Bioprocessing process that results in a simpler, lower-cost pathway for cellulosic ethanol. Click to enlarge. Source: DOE BESC General Motors Corp. and Mascoma Corp. have entered a strategic relationship to develop cellulosic ethanol based on Mascomas Consolidated Bioprocessing single-step biochemical conversion of non-grain biomass into...
https://www.greencarcongress.com/2008/05/gm-enters-strat.html?cid=113038288
GM Enters Strategic Relationship with Mascoma for Cellulosic Ethanol - Green Car Congress
Mascoma is developing a Consolidated Bioprocessing process that results in a simpler, lower-cost pathway for cellulosic ethanol. Click to enlarge. Source: DOE BESC General Motors Corp. and Mascoma Corp. have entered a strategic relationship to develop cellulosic ethanol based on Mascomas Consolidated Bioprocessing single-step biochemical conversion of non-grain biomass into...
https://www.greencarcongress.com/2008/05/gm-enters-strat.html
RNA repair - Humpath.com - Human pathology
References Mansfield SG, Chao H, Walsh CE. RNA repair using spliceosome-mediated RNA trans-splicing. Trends Mol Med. 2004 Jun;10(6):263-8. (...)
http://www.humpath.com/spip.php?article3823
0.022072</PDBx:fract transf...0.022072</PDBx:fract transf...
Structural and biochemical analysis of the phosphate donor specificity of the polynucleotide kinase component of the bacterial pnkphen1 RNA repair system ...
https://files.rcsb.org/view/4JT2-noatom.xml
Cellulosic Thickeners 75000mpas 45000cps 100000cps, Cellulosic Thickeners 75000mpas 45000cps 100000cps Suppliers and...Cellulosic Thickeners 75000mpas 45000cps 100000cps, Cellulosic Thickeners 75000mpas 45000cps 100000cps Suppliers and...
Cellulosic Thickeners 75000mpas 45000cps 100000cps, Wholesale Various High Quality Cellulosic Thickeners 75000mpas 45000cps 100000cps Products from Global Cellulosic Thickeners 75000mpas 45000cps 100000cps Suppliers and Cellulosic Thickeners 75000mpas 45000cps 100000cps Factory,Importer,Exporter at Alibaba.com.
http://www.alibaba.com/showroom/cellulosic-thickeners-75000mpas-45000cps-100000cps.html
Bistability of a coupled Aurora B kinase-phosphatase system in cell divisionBistability of a coupled Aurora B kinase-phosphatase system in cell division
Aurora B kinase, a key regulator of cell division, localizes to specific cellular locations, but the regulatory mechanisms responsible for phosphorylation of substrates located remotely from kinase enrichment sites are unclear. Here, we provide evidence that this activity at a distance depends on bo …
https://pubmed.ncbi.nlm.nih.gov/26765564/
MULTI-ENZYME FORMULA - 100 tabsMULTI-ENZYME FORMULA - 100 tabs
Buy MULTI-ENZYME FORMULA - 100 tabs Online.MULTI-ENZYMES FORMULA by Good ´n Natural is a powerful formula of digestive enzymes that are in charge of decomposing proteins, fats and carbohydrates in our body, these nutrients will help you
https://m.hsnstore.com/buy/good-n-natural/multi-enzyme-formula-100-tabs
love story - WriteWorklove story - WriteWork
Scholastic Writers Award 2013Group CThe final line of your story must be: And, to this day, I still dont know why she did it.by Lee Tse LynWho am I? Im Kestrel Night. Im in jail now for reasons I have yet to comprehend. Im sentenced for murder. ...
http://www.writework.com/essay/love-story-2
12f Blank Acetate Cels | Lightfoot Ltd
Make Your Selection Below for Quantity and Type of Hole if you wish to have the cels punched. Your choices are; Acme PUNCHED, Round PUNCHED or UnPUNCHED. Select Carefully Before You Place Your Order. ...
https://www.lightfootltd.com/content/12f-blank-acetate-cels
CellulosomeCellulosome
Clostridium cellulovorans Clostridium clariflavum Clostridium josui Clostridium papyrosolvens Clostridium thermocellum (treated ... The scaffoldin of some cellulosomes, an example being that of Clostridium thermocellum, contains a carbohydrate-binding module ... Dockerin Organelle Bayer, EA; Kenig, R; Lamed, R (1983). "Adherence of Clostridium thermocellum to cellulose". J. Bacteriol. ... has been derived from the study of Clostridium thermocellum. In the early 1980s, Raphael Lamed and Ed Bayer met at Tel Aviv ...
https://en.wikipedia.org/wiki/Cellulosome
Cellobiose phosphorylaseCellobiose phosphorylase
Alexander JK (June 1968). "Purification and specificity of cellobiose phosphorylase from Clostridium thermocellum". The Journal ...
https://en.wikipedia.org/wiki/Cellobiose_phosphorylase
Cellulose 1,4-beta-cellobiosidaseCellulose 1,4-beta-cellobiosidase
"Multidomain structure and cellulosomal localization of the Clostridium thermocellum cellobiohydrolase CbhA". Journal of ...
https://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase
Glycosidic bondGlycosidic bond
"Chemoenzymatic Synthesis of β-D Glucosides using Cellobiose Phosphorylase from Clostridium thermocellum". Advanced Synthesis & ...
https://en.wikipedia.org/wiki/Glycosidic_bond
Carbon capture and storageCarbon capture and storage
It would ultimately be ideal to exploit CO2 metabolizing bacterium Clostridium thermocellum. CO2 exothermically reacts with ... "CO2-fixing one-carbon metabolism in a cellulose-degrading bacterium Clostridium thermocellum". Proceedings of the National ...
https://en.wikipedia.org/wiki/Carbon_capture_and_storage
Glycoside hydrolase family 44Glycoside hydrolase family 44
... a glycoside hydrolase family 44 endoglucanase from Clostridium thermocellum". The Journal of Biological Chemistry. 282 (49): ...
https://en.wikipedia.org/wiki/Glycoside_hydrolase_family_44
Cellodextrin phosphorylaseCellodextrin phosphorylase
... orthophosphate glucosyltransferase from Clostridium thermocellum". J. Biol. Chem. 244 (2): 457-64. PMID 5773308. Portal: ...
https://en.wikipedia.org/wiki/Cellodextrin_phosphorylase
Edward A. BayerEdward A. Bayer
156, 828-36 (1983). Bayer, E. A., Kenig, R. & Lamed, R. Adherence of Clostridium thermocellum to cellulose. J. Bacteriol. 156, ... cellulase-containing complex in Clostridium thermocellum. J. Bacteriol. ... Biotechnology for biofuels production of a functional cell wall anchored minicellulosome by recombinant Clostridium ...
https://en.wikipedia.org/wiki/Edward_A._Bayer
Cohesin domainCohesin domain
The scaffolding component of the cellulolytic bacterium Clostridium thermocellum is a non-hydrolytic protein which organises ... "A cohesin domain from Clostridium thermocellum: the crystal structure provides new insights into cellulosome assembly". ...
https://en.wikipedia.org/wiki/Cohesin_domain
Carbohydrate-binding moduleCarbohydrate-binding module
One example is the CBM11 of Clostridium thermocellum Cel26A-Cel5E, this domain has been shown to bind both β-1,4-glucan and β-1 ... In anaerobic bacteria that degrade plant cell walls, exemplified by Clostridium thermocellum, the dockerin domains of the ... "The Family 11 Carbohydrate-binding Module of Clostridium thermocellum Lic26A-Cel5E Accommodates -1,4- and -1,3-1,4-Mixed Linked ... "Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS". FEMS Microbiol ...
https://en.wikipedia.org/wiki/Carbohydrate-binding_module
EF handEF hand
Clostridium thermocellum, 1daq​). Among all the structures reported to date, the majority of EF-hand motifs are paired either ...
https://en.wikipedia.org/wiki/EF_hand
SerpinSerpin
The precise role of most bacterial serpins remains obscure, although Clostridium thermocellum serpin localises to the ...
https://en.wikipedia.org/wiki/Serpin
Cellulosic ethanolCellulosic ethanol
One example is Clostridium thermocellum, which uses a complex cellulosome to break down cellulose and synthesize ethanol. ... However, C. thermocellum also produces other products during cellulose metabolism, including acetate and lactate, in addition ... Demain AL, Newcomb M, Wu JH (March 2005). "Cellulase, clostridia, and ethanol". Microbiology and Molecular Biology Reviews. 69 ... Instead of sugar fermentation with yeast, this process uses Clostridium ljungdahlii bacteria. This microorganism will ingest ...
https://en.wikipedia.org/wiki/Cellulosic_ethanol
Caldicellulosiruptor besciiCaldicellulosiruptor bescii
Bi-functional acetaldehyde/alcohol dehydrogenase genes from Clostridium thermocellum allow for the conversion of sugars to ...
https://en.wikipedia.org/wiki/Caldicellulosiruptor_bescii
Cellulose 1,4-beta-cellobiosidase (reducing end)Cellulose 1,4-beta-cellobiosidase (reducing end)
The CelS enzyme from Clostridium thermocellum is the most abundant subunit of the cellulosome formed by the organism. Barr BK, ...
https://en.wikipedia.org/wiki/Cellulose_1,4-beta-cellobiosidase_(reducing_end)
CaldibacillusCaldibacillus
Cellulose-Degrading Coculture with Clostridium thermocellum". Applied and Environmental Microbiology. 81 (16): 5567-5573. doi: ...
https://en.wikipedia.org/wiki/Caldibacillus
Acetivibrio thermocellusAcetivibrio thermocellus
JGI: Archived 2007-12-21 at the Wayback Machine Clostridium thermocellum ATCC 27405 Cellulase, Clostridia, and Ethanol Ibid. ... Characterization of Clostridium thermocellum JW20 U.S. Department of Energy: Archived 2007-04-27 at the Wayback Machine ... Cellulosic Ethanol Type strain of Clostridium thermocellum at BacDive - the Bacterial Diversity Metadatabase v t e (Webarchive ...
https://en.wikipedia.org/wiki/Acetivibrio_thermocellus
ClostridiumClostridium
... butyricum converts glycerol to 1,3-propanediol. Genes from Clostridium thermocellum have been inserted into ... Mixtures of Clostridium species, such as Clostridium beijerinckii, Clostridium butyricum, and species from other genera have ... Clostridium difficile, now placed in Clostridioides. Clostridium histolyticum, now placed in Hathewaya. Clostridium sordellii, ... Clostridium welchii and Clostridium tetani respond to sulfonamides. Clostridia are also susceptible to tetracyclines, ...
https://en.wikipedia.org/wiki/Clostridium
CellulaseCellulase
Zverlov VV, Schantz N, Schwarz WH (August 2005). "A major new component in the cellulosome of Clostridium thermocellum is a ... For example, Clostridium cellulolyticum produces 13 GH9 modular cellulases containing a different number and arrangement of ... "Characterization of all family-9 glycoside hydrolases synthesized by the cellulosome-producing bacterium Clostridium ...
https://en.wikipedia.org/wiki/Cellulase
DockerinDockerin
"Cohesin-dockerin interactions within and between Clostridium josui and Clostridium thermocellum: binding selectivity between ... "Structural characterization of type II dockerin module from the cellulosome of Clostridium thermocellum: calcium-induced ... "Structural characterization of type II dockerin module from the cellulosome of Clostridium thermocellum: calcium-induced ...
https://en.wikipedia.org/wiki/Dockerin
Fibrobacter succinogenesFibrobacter succinogenes
This unique metabolism differs form other model cellulose degraders like Clostridium thermocellum and Trichoderma reesei which ...
https://en.wikipedia.org/wiki/Fibrobacter_succinogenes
Glycoside hydrolase family 48Glycoside hydrolase family 48
The C-terminal domain belongs to this family shows similarity to a cellulase from Clostridium thermocellum (CelS), which acts ...
https://en.wikipedia.org/wiki/Glycoside_hydrolase_family_48
List of Clostridium speciesList of Clostridium species
Clostridium aldrichii, C. alkalicellulosi, C. clariflavum, C. straminisolvens and C. thermocellum, reassigned in 2019. Genus ... Clostridium aceticum Clostridium acetireducens Clostridium acetobutylicum Clostridium acidisoli Clostridium aciditolerans ... Clostridium aestuarii Clostridium akagii Clostridium algidicarnis Clostridium algifaecis Clostridium algoriphilum Clostridium ... Clostridium novyi Clostridium oceanicum Clostridium oryzae Clostridium paradoxum Clostridium paraputrificum Clostridium pascui ...
https://en.wikipedia.org/wiki/List_of_Clostridium_species
PathemaPathema
... kluyveri Clostridium novyi Clostridium perfringens Clostridium phytofermentans Clostridium tetani Clostridium thermocellum ... Pathema-Clostridium Clostridium acetobutylicum Clostridium botulinum Clostridium butyricum Clostridium difficile Clostridium ... Clostridium botulinum, Burkholderia mallei, Burkholderia pseudomallei, Clostridium perfringens, and Entamoeba histolytica) ...
https://en.wikipedia.org/wiki/Pathema
List of MeSH codes (B03)List of MeSH codes (B03)
Clostridium tetanomorphum MeSH B03.300.390.400.200.770 - Clostridium thermocellum MeSH B03.300.390.400.200.800 - Clostridium ... Clostridium tetanomorphum MeSH B03.510.415.400.200.770 - Clostridium thermocellum MeSH B03.510.415.400.200.800 - Clostridium ... Clostridium botulinum type G MeSH B03.300.390.400.200.180 - Clostridium butyricum MeSH B03.300.390.400.200.200 - Clostridium ... Clostridium botulinum type G MeSH B03.510.415.400.200.180 - Clostridium butyricum MeSH B03.510.415.400.200.200 - Clostridium ...
https://en.wikipedia.org/wiki/List_of_MeSH_codes_(B03)
List of long species namesList of long species names
nov.; Reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100- ... 2018, Hungateiclostridium thermocellum (Viljoen et al. 1926) Zhang et al. 2018, Hungateiclostridium cellulolyticum (Patel et al ... "Species: Clostridium thermosulfurigenes". lpsn.dsmz.de. Archived from the original on 2021-02-26. Retrieved 2021-03-22. Nogi, ... Originally described as Clostridium glycyrrhizinilyticum (31 letters) and later reclassified in genus Mediterraneibacter. Its ...
https://en.wikipedia.org/wiki/List_of_long_species_names
Difference between revisions of "Clostridium thermocellum" - microbewiki
"Clostridium Thermocellum. International Programme on Chemical Safety Poisons Information Monograph 858 Bacteria". World Health ... Like most species from the Clostridium genus, C. thermocellum is a bacteria that has a rod-like shape for its cell body. It is ... The other prominent Clostridium species that exhibits pathogenic effects is Clostridium tetani. C. tetani primarily infect ... Difference between revisions of "Clostridium thermocellum". From MicrobeWiki, the student-edited microbiology resource ...
https://microbewiki.kenyon.edu/index.php?title=Clostridium_thermocellum&diff=prev&oldid=11018
RCSB PDB - 2C7F: The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5...RCSB PDB - 2C7F: The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5...
... from Clostridium thermocellum in complex with 1,5-alpha-L-Arabinotriose. ... The anaerobic bacterium Clostridium thermocellum, a highly efficient plant cell wall degrader, possesses a single alpha-L- ... The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5-alpha-L- ... from Clostridium Thermocellum.. Taylor, E.J., Smith, N.L., Turkenburg, J.P., DSouza, S., Gilbert, H.J., Davies, G.J.. (2006) ...
https://www.rcsb.org/structure/2c7f
Domain assignment for gi|385779772|ref|YP 005688937.1| from Clostridium thermocellum DSM 1313Domain assignment for gi|385779772|ref|YP 005688937.1| from Clostridium thermocellum DSM 1313
... from Clostridium thermocellum DSM 1313. Plus protein sequence and external database links. ... Domain assignment for gi,385779772,ref,YP_005688937.1, from Clostridium thermocellum DSM 1313. Domain architecture ... Peptidoglycan-binding lysin domain [Clostridium thermocellum DSM 1313]. Sequence. ...
https://supfam.mrc-lmb.cam.ac.uk/SUPERFAMILY/cgi-bin/gene.cgi?genome=xRg
Product selectivity shifts in Clostridium thermocellum in the presence of compressed solvents<...
Product selectivity shifts in Clostridium thermocellum in the presence of compressed solvents. / Berberich, Jason A.; Knutson, ... Product selectivity shifts in Clostridium thermocellum in the presence of compressed solvents. In: Industrial and Engineering ... Dive into the research topics of Product selectivity shifts in Clostridium thermocellum in the presence of compressed solvents ... Product selectivity shifts in Clostridium thermocellum in the presence of compressed solvents. Industrial and Engineering ...
https://scholars.uky.edu/en/publications/product-selectivity-shifts-in-clostridium-thermocellum-in-the-pre
Genome-scale metabolic analysis of Clostridium thermocellum for bioethanol production | BMC Systems Biology | Full TextGenome-scale metabolic analysis of Clostridium thermocellum for bioethanol production | BMC Systems Biology | Full Text
Clostridium thermocellum (ATCC 27405) is a thermophilic anaerobe that is both cellulolytic and ethanologenic, meaning that it ... thermocellum and facilitate efforts to engineer C. thermocellum for biofuel production. The model consists of 577 reactions ... Here we present a genome-scale model of C. thermocellum metabolism, i SR432, for the purpose of establishing a computational ... thermocellum and establish a strong foundation for rational strain design. In addition, we are able to draw some important ...
https://bmcsystbiol.biomedcentral.com/articles/10.1186/1752-0509-4-31
Decoding biomass-sensing regulons of Clostridium thermocellum alternative sigma factors----中国科学院青岛生物能源与过Decoding biomass-sensing regulons of Clostridium thermocellum alternative sigma factors----中国科学院青岛生物能源与过
Clostridium thermocellum is a thermophile capable of converting cellulosic wastes into fermentation products such as ethanol. ... During the last several years, our research group has focused on demonstrating that individual C. thermocellum sI factors ... During the course of our efforts to gain knowledge about the biomass-sensing mechanisms in C. thermocellum, our research groups ... polysaccharide-sensing systems of cellulolytic clostridia; transcriptional regulation by alternative sigma factors in ...
http://www.qibebt.cas.cn/xwzx/nbjl/201711/t20171116_4895100.html
IJMS | Special Issue : Carbohydrate-Active Enzymes: Structure, Activity and Reaction ProductsIJMS | Special Issue : Carbohydrate-Active Enzymes: Structure, Activity and Reaction Products
While the optimal temperature of the Clostridium thermocellum cellulosome is 70 °C, C. thermocellum β-glucosidase A is almost ... Directed Evolution of Clostridium thermocellum β-Glucosidase A Towards Enhanced Thermostability by Shahar Yoav ... Upon the addition of the thermostable mutant to the C. thermocellum secretome for subsequent hydrolysis of microcrystalline ...
https://www.mdpi.com/journal/ijms/special_issues/carbohydrate-active_enzymes
Public defences of doctoral theses | KTHPublic defences of doctoral theses | KTH
2022-11-08T09:00:00.000+01:00 2022-11-08T09:00:00.000+01:00 Insights into the metabolism of Clostridium thermocellum for ... Insights into the metabolism of Clostridium thermocellum for cellulosic ethanol production (Public defences of doctoral theses) ...
https://www.kth.se/en/om/mot/kalender/disputationer?date=2022-09-26&length=97
PDB 1cecPDB 1cec
Clostridium thermocellum. Primary Citation. A common protein fold and similar active site in two distinct families of beta- ...
https://www.cathdb.info/pdb/1cec
SeqTU: A Web Server for Identification of Bacterial Transcription Units | Scientific ReportsSeqTU: A Web Server for Identification of Bacterial Transcription Units | Scientific Reports
While good performance was achieved by the method on Escherichia coli and Clostridium thermocellum, substantial work is needed ... The test results of this strategy for calling TUs in E. coli and Clostridium thermocellum suggest that this strategy works very ... While good performance was achieved by the method on Escherichia coli and Clostridium thermocellum, substantial work is needed ... In all the Clostridium thermocellum RNA-seq datasets, the trained predictors achieved prediction sensitivities over 0.9. The ...
https://www.nature.com/articles/srep43925?error=cookies_not_supported&code=0299dd62-b543-4783-83ad-6a6514b3190e
SCOPe 2.08: Domain d1zgha1: 1zgh A:165-226SCOPe 2.08: Domain d1zgha1: 1zgh A:165-226
Species Clostridium thermocellum [TaxId:1515] [117236] (2 PDB entries). Uniprot Q4HJX2; 47% sequence identity; truncated C- ... PDB Description: methionyl-trna formyltransferase from clostridium thermocellum. PDB Compounds: (A:) Methionyl-tRNA ... d1zgha1 b.46.1.1 (A:165-226) Methionyl-tRNAfmet formyltransferase, C-terminal domain {Clostridium thermocellum [TaxId: 1515]} ...
http://scop.berkeley.edu/sid=d1zgha1
In vivo und in vitro Interaktionen haloarchaealer Gasvesikelproteine - TUprints In vivo und in vitro Interaktionen haloarchaealer Gasvesikelproteine - TUprints
For this purpose, the cellulose binding domain (CBD) from Clostridium thermocellum was used, as it is stable even under high ... Hierfür wurde die Cellulose-Bindedomäne (CBD) aus Clostridium thermocellum verwendet, da diese auch unter Hochsalzbedingungen ...
https://tuprints.ulb.tu-darmstadt.de/11850/
Fedøy AE - Search Results - PubMedFedøy AE - Search Results - PubMed
The complex structures of isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila suggest a new ...
https://pubmed.ncbi.nlm.nih.gov/?term=Fed%C3%B8y+AE&cauthor_id=26913091
Spatially implicit and spatially explicit models of bacterial cellulose degradation | Fields Institute for Research in...Spatially implicit and spatially explicit models of bacterial cellulose degradation | Fields Institute for Research in...
Clostridium thermocellum is a bacteria that is able to directly convert cellulose into ethanol (and other by products). These ... spatially implicit reactor scale model for cellulose degradation by C.thermocellum biofilms. The ODE can be studied with ...
http://www.fields.utoronto.ca/talks/spatially-implicit-and-spatially-explicit-models-bacterial-cellulose-degradation
Biohydrogen | Bioenergy | NRELBiohydrogen | Bioenergy | NREL
Comparison of transcriptional profiles of Clostridium thermocellum grown on cellobiose and pretreated yellow poplar using RNA- ... To lower biomass feedstock costs, NREL developed the cellulose-degrader Clostridium thermocellum to directly convert cellulose/ ... In one approach to address this barrier, we transform into both hosts an O2-tolerant hydrogenase either from Clostridium ... thermocellum to direct more cellular flux toward hydrogen. Moreover, additional hydrogen can be produced from the fermentation ...
https://www.nrel.gov/bioenergy/biohydrogen.html
Frontiers | Single-Cell RNA Sequencing of Plant-Associated Bacterial CommunitiesFrontiers | Single-Cell RNA Sequencing of Plant-Associated Bacterial Communities
... strand-specific RNA-seq data using machine learning reveals the structures of transcription units in Clostridium thermocellum. ...
https://www.frontiersin.org/articles/10.3389/fmicb.2019.02452/full
Herbinix luporum sp. nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor |...Herbinix luporum sp. nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor |...
Koeck D. E., Zverlov V. V., Liebl W., Schwarz W. H. 2014b; Comparative genotyping of Clostridium thermocellum strains isolated ... Jeong H., Yi H., Sekiguchi Y., Muramatsu M., Kamagata Y., Chun J. 2004; Clostridium jejuense sp. nov., isolated from soil. Int ... Chemically defined minimal medium for growth of the anaerobic cellulolytic thermophile Clostridium thermocellum . Appl Environ ... Mechichi T., Labat M., Garcia J. L., Thomas P., Patel B. K. 1999; Characterization of a new xylanolytic bacterium, Clostridium ...
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001324
Production Optimization of Fungal Cellulase and its Impact on Ruminal Degradability and Fermentation of DietProduction Optimization of Fungal Cellulase and its Impact on Ruminal Degradability and Fermentation of Diet
Adsorption of Clostridium thermocellum cellulases onto pretreated mixed hardwood, avicel and lignin. Biotechnol. Bioeng., 42: ... Product selectivity shifts in Clostridium thermocellum in the presence of compressed solvents. Ind. Eng. Chem. Res., 39: 4500- ... Carboxymethylcellulase and Avicelase activities from a cellulolytic Clostridium strain A11. Curr. Microbiol., 30: 305-312.. ...
https://scialert.net/fulltext/?doi=ijds.2019.61.68&org=11#ref
BESC study seeks natures best biocatalysts for biofuel production | ORNLBESC study seeks nature's best biocatalysts for biofuel production | ORNL
The microbe Clostridium thermocellum (stained green), seen growing on a piece of poplar biomass, is among several ... Their analysis demonstrated that under carefully controlled conditions, a microbe called Clostridium thermocellum is twice as ...
https://www.ornl.gov/news/besc-study-seeks-natures-best-biocatalysts-biofuel-production
Uploads by Christopher M Gowen - OpenWetWareUploads by Christopher M Gowen - OpenWetWare
Genome-scale constraint-based metabolic model of Clostridium thermocellum 13:47, 18 March 2009. Purge valve.png (file). 8 KB. ...
https://openwetware.org/wiki/Special:ListFiles/Christopher_M_Gowen
Glycosciences.de - Glycosciences.DB entry 22419Glycosciences.de - Glycosciences.DB entry 22419
PDB ID 7x0h: Crystal structure of sugar binding protein CbpA complexed wtih glucose from Clostridium thermocellum ...
http://www.glycosciences.de/database/start.php?action=explore_linucsid&linucsid=22419&show=16385
SMART: FN3 domain annotationSMART: FN3 domain annotation
The crystal structure of a Fn3-like protein from Clostridium thermocellum. 3mtr. Crystal structure of the Ig5-FN1 tandem of ... cohesin and fibronectin type-III double module construct from the Clostridium perfringens glycoside hydrolase GH84C. ...
https://smart.embl.de/smart/do_annotation.pl?DOMAIN=FN3&START=30728&END=30814&E_VALUE=6.24e-12&TYPE=SMART&BLAST=VDPPGRPEVTDVTRSTVSLIWSAPVYDGGSKVVGYIIERKPVSEVGDGRWLKCNYTIVSDNFFTVTALSEGDTYEFRVLAKNAAGVI
컨텐츠 | Nature Portfolio
... the X-ray crystal structures of PCAT1 from Clostridium thermocellum in the absence and presence of ATP are reported. doi: ...
https://www.natureasia.com/ko-kr/nature/toc/523/7561
Nagiza Samatova - Google ScholarNagiza Samatova - Google Scholar
Impact of pretreated switchgrass and biomass carbohydrates on Clostridium thermocellum ATCC 27405 cellulosome composition: a ...
https://scholar.google.nl/citations?user=vukA8JAAAAAJ&hl=en&oe=ASCII
Metasecretome-selective phage display approach for mining the functional potential of a rumen microbial community | BMC...Metasecretome-selective phage display approach for mining the functional potential of a rumen microbial community | BMC...
... formerly Clostridium cellulolyticum), Ru. josui (formerly C. josui) and Ru. thermocellum (formerly C. thermocellum) [22]. In ... Bayer EA, Setter E, Lamed R: Organization and distribution of the cellulosome in Clostridium thermocellum. J Bacteriol. 1985, ... Lamed R, Setter E, Bayer EA: Characterization of a cellulose-binding, cellulase-containing complex in Clostridium thermocellum ... thermocellum and C. cellulovorans, and R. flavefaciens FD1 [58-60]. A striking difference in comparison with reports from ...
https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-15-356
IUCr) ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRFIUCr) ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF
... a seleno-methione-derivative of the feruloyl esterase module of xylanase 10B from Clostridium thermocellum (FAE) was ...
https://journals.iucr.org/s/issues/2018/04/00/ig5055/
Plus itPlus it
Here we deconvolute a highly efficient cellulose-degrading consortium (SEM1b) that is co-dominated by Clostridium ( ... Ruminiclostridium) thermocellum- and multiple heterogenic strains affiliated to C. proteolyticus. Metagenomic analysis of SEM1b ... thermocellum- or Thermotogae-affiliated populations that are historically co-located. HGT material included whole saccharolytic ...
https://www.biorxiv.org/content/10.1101/280602v1
Study seeks natures best biocatalysts for biofuel productionStudy seeks nature's best biocatalysts for biofuel production
The microbe Clostridium thermocellum (stained green), seen growing on a piece of poplar biomass, is among several ... Their analysis demonstrated that under carefully controlled conditions, a microbe called Clostridium thermocellum is twice as ...
https://phys.org/news/2016-01-nature-biocatalysts-biofuel-production.html
GlyConnectGlyConnect
Cellulosomal-scaffolding protein A / Clostridium thermocellum Q06851 # Export GlyConnect protein list related to protein with ... The nature of the carbohydrate-peptide linkage region in glycoproteins from the cellulosomes of Clostridium thermocellum and ... The nature of the carbohydrate-peptide linkage region in glycoproteins from the cellulosomes of Clostridium thermocellum and ... Primary structure of O-linked carbohydrate chains in the cellulosome of different Clostridium thermocellum strains. (1991 - ...
https://glyconnect.expasy.org/o-monosaccharide/proteins?n=0
Novel bacterium produces butanol directly from cellulose - Green Car Congress
Microbiology on butanol production from crystalline cellulose by co-cultured Clostridium thermocellum and Clostridium ...
https://www.greencarcongress.com/2011/08/tu103-20110828.html
ATCCOrganismAnaerobicThermophilic bacteriumCelluloseGenusAcetobutylicumThermophileBacteria grampositivaGram-positiveGenomeMetabolicBacillusPerfringensDifficileBiomassMetabolismStrainsCellularMechanismsTranscriptionPeptidoglycanEnzymesCellComplexPresence
ATCC1
  • Clostridium thermocellum (ATCC 27405) is a thermophilic anaerobe that is both cellulolytic and ethanologenic, meaning that it can directly use the plant sugar, cellulose, and biochemically convert it to ethanol. (biomedcentral.com)
Organism2
  • C. thermocellum is an anaerobic and thermophilic organism that produces spores. (kenyon.edu)
  • While good performance was achieved by the method on Escherichia coli and Clostridium thermocellum , substantial work is needed to make the program generally applicable to all bacteria, knowing that the program requires organism specific information. (nature.com)
Anaerobic2
  • The anaerobic bacterium Clostridium thermocellum, a highly efficient plant cell wall degrader, possesses a single alpha-L-arabinofuranosidase (EC 3.2.1.55), CtAraf51A, located in GH51 (glycoside hydrolase family 51). (rcsb.org)
  • The anaerobic thermophilic bacterium Clostridium thermocellum, which produces ethanol, acetate, and lactate, was used in the biphasic incubations. (uky.edu)
Thermophilic bacterium1
  • Clostridium thermocellum is a thermophilic bacterium recognized for its natural ability to effectively deconstruct cellulosic biomass. (nrel.gov)
Cellulose9
  • Because of its ability to break down cellulose, it is highly likely that C. thermocellum can be isolated from many plant organisms. (kenyon.edu)
  • Because C. thermocellum is known as a degrader of cellulose, its DNA contains specific nucleotide sequences that make up the genes that encode for the system of enzymes that are necessary for cellulose degradation. (kenyon.edu)
  • Hierfür wurde die Cellulose-Bindedomäne (CBD) aus Clostridium thermocellum verwendet, da diese auch unter Hochsalzbedingungen stabil ist. (tu-darmstadt.de)
  • Clostridium thermocellum is a bacteria that is able to directly convert cellulose into ethanol (and other by products). (utoronto.ca)
  • In this talk we first present a very simple, spatially implicit reactor scale model for cellulose degradation by C.thermocellum biofilms. (utoronto.ca)
  • To lower biomass feedstock costs, NREL developed the cellulose-degrader Clostridium thermocellum to directly convert cellulose/hemicellulose to hydrogen without relying on an expensive hydrolytic enzyme cocktail in a consolidated bioprocessing configuration. (nrel.gov)
  • Researchers from the Tokyo University of Agriculture earlier this year reported in the journal Applied and Environmental Microbiology on butanol production from crystalline cellulose by co-cultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4. (greencarcongress.com)
  • The sequence analysis of the amplicons revealed that CBMs of 7 of the isolates including the isolate 7-9-1 with the highest capacity of cellulose degradation in solid medium have 100% identity in both nucleotide and amino acid sequences to CipB of Clostridium thermocellum in the compared regions. (tubitak.gov.tr)
  • Cristaux de cellulase, enzyme purifiée de Clostridium thermocellum permettant la digestion de la cellulose. (pasteur.fr)
Genus3
  • Like most species from the Clostridium genus, C. thermocellum is a bacteria that has a rod-like shape for its cell body. (kenyon.edu)
  • were distinguished as separate species of the genus Clostridium . (springeropen.com)
  • Type species of the genus CLOSTRIDIUM , a gram-positive bacteria in the family Clostridiaceae. (bvsalud.org)
Acetobutylicum1
  • In one approach to address this barrier, we transform into both hosts an O2-tolerant hydrogenase either from Clostridium acetobutylicum (a FeFe-hydrogenase into the green algae) or Rubrivivax gelatinosus (a NiFe-hydrogenase into the cyanobacterium), designed to continuously produce hydrogen during the day time. (nrel.gov)
Thermophile1
  • Clostridium thermocellum is a thermophile capable of converting cellulosic wastes into fermentation products such as ethanol. (cas.cn)
Bacteria grampositiva1
  • Especie tipo del género CLOSTRIDIUM, bacteria grampositiva de la familia Clostridiaceae. (bvsalud.org)
Gram-positive1
  • Aside from the general internal framework of the cell, C. thermocellum is a gram-positive bacteria. (kenyon.edu)
Genome3
  • The number of nucleotides present in the genome of C. thermocellum has been discovered and reported to be at 3,843,301 base pairs which makes up 3307 genes [1] . (kenyon.edu)
  • Here we present a genome-scale model of C. thermocellum metabolism, i SR432, for the purpose of establishing a computational tool to study the metabolic network of C. thermocellum and facilitate efforts to engineer C. thermocellum for biofuel production. (biomedcentral.com)
  • In addition, we are able to draw some important conclusions regarding the underlying metabolic mechanisms for observed behaviors of C. thermocellum and highlight remaining gaps in the existing genome annotations. (biomedcentral.com)
Metabolic2
  • To improve hydrogen molar yield, our group has developed the genetic toolkit, an enabling technology to manipulate the metabolic pathways in C. thermocellum to direct more cellular flux toward hydrogen. (nrel.gov)
  • Together, this aids future metabolic engineering attempts aimed to improve C. thermocellum as a cell factory for sustainable and efficient production of ethanol from lignocellulosic material through consolidated bioprocessing with minimal pretreatment. (sciencegate.app)
Bacillus2
  • During the course of our efforts to gain knowledge about the biomass-sensing mechanisms in C. thermocellum , our research groups discovered a collection of eight alternative s factors that are related to the Bacillus subtilis s I factor. (cas.cn)
  • The original isolate of Clostridium bifermentans was named Bacillus bifermentans sporogenes (Clark and Hall 1937 ), and later re-named B. bifermentans (Bergey et al. (springeropen.com)
Perfringens1
  • Israr M, Lv G, Xu S, Li Y, Ding S, Zhao B, Ju J (2019) Biochemical characterization and mutational analysis of alanine racemase from Clostridium perfringens J Biosci Bioeng 128 149-155. (unipr.it)
Difficile1
  • 2014) Structural and biochemical analyses of alanine racemase from the multidrug-resistant Clostridium difficile strain 630 Acta Crystallogr D Biol Crystallogr. (unipr.it)
Biomass1
  • The microbe Clostridium thermocellum (stained green), seen growing on a piece of poplar biomass, is among several microorganisms recently evaluated in a BioEnergy Science Center comparative study. (ornl.gov)
Metabolism1
  • IMPORTANCE Increased understanding of the central metabolism of C. thermocellum is important from a fundamental as well as from a sustainability and industrial perspective. (sciencegate.app)
Strains1
  • Annotation of all C. proteolyticus genotypes (two strains and one MAG) revealed their genetic acquisition of various carbohydrate-active enzymes (CAZymes), presumably derived from horizontal gene transfer (HGT) events involving C. thermocellum- or Thermotogae-affiliated populations that are historically co-located. (biorxiv.org)
Cellular2
  • The general interior cellular structure of C. thermocellum is similar to most rod-shaped bacteria. (kenyon.edu)
  • By incorporating genomic sequence data, network topology, and experimental measurements of enzyme activities and metabolite fluxes, we have generated a model that is reasonably accurate at predicting the cellular phenotype of C. thermocellum and establish a strong foundation for rational strain design. (biomedcentral.com)
Mechanisms1
  • These results indicate that the four investigated PP i sources individually and combined play no significant PP i -supplying role and the true source(s) of PP i , or alternative phosphorylating mechanisms, that drive glycolysis in C. thermocellum remain(s) elusive. (sciencegate.app)
Transcription1
  • In regards to genomic organization, C. thermocellum like all other prokaryotic organisms lack a true nucleus for DNA storage and transcription. (kenyon.edu)
Peptidoglycan1
  • The type of peptidoglycan that forms the cell wall of C. thermocellum is known as murein which protects its single bilayer membrane from high turgor pressure and also provides the cell its shape and rigidity. (kenyon.edu)
Enzymes1
  • Their analysis demonstrated that under carefully controlled conditions, a microbe called Clostridium thermocellum is twice as effective as fungal enzymes used by industry today. (ornl.gov)
Cell1
  • I constructed a reporter system that displays the Clostridium thermocellum endoglucanase (Cel8A) on the cell surface through the noncovalent cell wall binding motif, LysM. (escholarship.org)
Complex2
  • 2C7F: The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5-alpha-L-Arabinotriose. (rcsb.org)
  • The complex structures of isocitrate dehydrogenase from Clostridium thermocellum and Desulfotalea psychrophila suggest a new active site locking mechanism. (nih.gov)
Presence1
  • here, the X-ray crystal structures of PCAT1 from Clostridium thermocellum in the absence and presence of ATP are reported. (natureasia.com)
Difference between revisions of Clostridium thermocellum - microbewiki
Difference between revisions of "Clostridium thermocellum" - microbewiki (microbewiki.kenyon.edu)
RCSB PDB - 2C7F: The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5...
RCSB PDB - 2C7F: The Structure of a family 51 arabinofuranosidase, Araf51, from Clostridium thermocellum in complex with 1,5... (rcsb.org)
IJMS | Special Issue : Carbohydrate-Active Enzymes: Structure, Activity and Reaction Products
IJMS | Special Issue : Carbohydrate-Active Enzymes: Structure, Activity and Reaction Products (mdpi.com)
SCOPe 2.08: Domain d1zgha1: 1zgh A:165-226
SCOPe 2.08: Domain d1zgha1: 1zgh A:165-226 (scop.berkeley.edu)
Frontiers | Single-Cell RNA Sequencing of Plant-Associated Bacterial Communities
Frontiers | Single-Cell RNA Sequencing of Plant-Associated Bacterial Communities (frontiersin.org)
Herbinix luporum sp. nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor |...
Herbinix luporum sp. nov., a thermophilic cellulose-degrading bacterium isolated from a thermophilic biogas reactor |... (microbiologyresearch.org)
Production Optimization of Fungal Cellulase and its Impact on Ruminal Degradability and Fermentation of Diet
Production Optimization of Fungal Cellulase and its Impact on Ruminal Degradability and Fermentation of Diet (scialert.net)
BESC study seeks nature's best biocatalysts for biofuel production | ORNL
BESC study seeks nature's best biocatalysts for biofuel production | ORNL (ornl.gov)
SMART: FN3 domain annotation
SMART: FN3 domain annotation (smart.embl.de)
Nagiza Samatova - Google Scholar
Nagiza Samatova - Google Scholar (scholar.google.nl)
SCOPe 2.08: Domain d4dh2a2: 4dh2 A:163-164
SCOPe 2.08: Domain d4dh2a2: 4dh2 A:163-164 (scop.berkeley.edu)
Study seeks nature's best biocatalysts for biofuel production
Study seeks nature's best biocatalysts for biofuel production (phys.org)
Biofilm formation by designed co-cultures of Caldicellulosiruptor species as a means to improve hydrogen productivity |...
Biofilm formation by designed co-cultures of Caldicellulosiruptor species as a means to improve hydrogen productivity |... (biotechnologyforbiofuels.biomedcentral.com)
Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea | BMC...
Genome sequencing and identification of cellulase genes in Bacillus paralicheniformis strains from the Red Sea | BMC... (bmcmicrobiol.biomedcentral.com)
Primary Structure of the Carbohydrate-Binding Modules in Various Cellu by MELEK ÖZKAN and GÜLAY ÖZCENGİZ
"Primary Structure of the Carbohydrate-Binding Modules in Various Cellu" by MELEK ÖZKAN and GÜLAY ÖZCENGİZ (journals.tubitak.gov.tr)
The ability of Clostridium bifermentans strains to lactic acid biosynthesis in various environmental conditions | SpringerPlus ...
The ability of Clostridium bifermentans strains to lactic acid biosynthesis in various environmental conditions | SpringerPlus ... (springerplus.springeropen.com)
Laminaribiose Oligosaccharide | Megazyme
Laminaribiose Oligosaccharide | Megazyme (megazyme.com)
Stephen S. Fong, Ph.D. Professor, Department of Chemical and Life Science Engineering - Expert with VCU College of Engineering ...
Stephen S. Fong, Ph.D. Professor, Department of Chemical and Life Science Engineering - Expert with VCU College of Engineering ... (expertfile.com)
Bacillus subtilis as a Candidate for Consolidated Bioprocessing: Microbial Cell Wall Engineering for the Display of...
Bacillus subtilis as a Candidate for Consolidated Bioprocessing: Microbial Cell Wall Engineering for the Display of... (escholarship.org)
In-Fusion Cloning citations
In-Fusion Cloning citations (takarabio.com)