A species of gram-positive, anaerobic bacteria in the family Clostridiaceae frequently used for the study of ENZYMES.
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
A class of enzymes that catalyze oxidation-reduction reactions of amino acids.
An amino acid produced in the urea cycle by the splitting off of urea from arginine.
An element with the atomic symbol Se, atomic number 34, and atomic weight 78.96. It is an essential micronutrient for mammals and other animals but is toxic in large amounts. Selenium protects intracellular structures against oxidative damage. It is an essential component of GLUTATHIONE PEROXIDASE.
A common inhabitant of the colon flora in human infants and sometimes in adults. It produces a toxin that causes pseudomembranous enterocolitis (ENTEROCOLITIS, PSEUDOMEMBRANOUS) in patients receiving antibiotic therapy.
Infections with bacteria of the genus CLOSTRIDIUM.
A species of anaerobic, gram-positive, rod-shaped bacteria in the family Clostridiaceae that produces proteins with characteristic neurotoxicity. It is the etiologic agent of BOTULISM in humans, wild fowl, HORSES; and CATTLE. Seven subtypes (sometimes called antigenic types, or strains) exist, each producing a different botulinum toxin (BOTULINUM TOXINS). The organism and its spores are widely distributed in nature.
A genus of gram-positive, anaerobic, coccoid bacteria that is part of the normal flora of humans. Its organisms are opportunistic pathogens causing bacteremias and soft tissue infections.
A group of enzymes that catalyze the reduction of 1-pyrroline carboxylate to proline in the presence of NAD(P)H. Includes both the 2-oxidoreductase (EC 1.5.1.1) and the 5-oxidoreductase (EC 1.5.1.2). The former also reduces 1-piperidine-2-carboxylate to pipecolate and the latter also reduces 1-pyrroline-3-hydroxy-5-carboxylate to hydroxyproline.
An acute inflammation of the INTESTINAL MUCOSA that is characterized by the presence of pseudomembranes or plaques in the SMALL INTESTINE (pseudomembranous enteritis) and the LARGE INTESTINE (pseudomembranous colitis). It is commonly associated with antibiotic therapy and CLOSTRIDIUM DIFFICILE colonization.
A species of gram-positive bacteria in the family Clostridiaceae, used for the industrial production of SOLVENTS.
One of the three domains of life (the others being Eukarya and ARCHAEA), also called Eubacteria. They are unicellular prokaryotic microorganisms which generally possess rigid cell walls, multiply by cell division, and exhibit three principal forms: round or coccal, rodlike or bacillary, and spiral or spirochetal. Bacteria can be classified by their response to OXYGEN: aerobic, anaerobic, or facultatively anaerobic; by the mode by which they obtain their energy: chemotrophy (via chemical reaction) or PHOTOTROPHY (via light reaction); for chemotrophs by their source of chemical energy: CHEMOLITHOTROPHY (from inorganic compounds) or chemoorganotrophy (from organic compounds); and by their source for CARBON; NITROGEN; etc.; HETEROTROPHY (from organic sources) or AUTOTROPHY (from CARBON DIOXIDE). They can also be classified by whether or not they stain (based on the structure of their CELL WALLS) with CRYSTAL VIOLET dye: gram-negative or gram-positive.
A family of nucleocytoplasmic, large, double-stranded DNA viruses with extremely complex genomes.
Viruses whose nucleic acid is DNA.
The relationships of groups of organisms as reflected by their genetic makeup.
Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.
DNA sequences encoding RIBOSOMAL RNA and the segments of DNA separating the individual ribosomal RNA genes, referred to as RIBOSOMAL SPACER DNA.
All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.
A publication issued at stated, more or less regular, intervals.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)
Group composed of associates of same species, approximately the same age, and usually of similar rank or social status.
A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.
Recording of visual and sometimes sound signals on magnetic tape.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.
An organized procedure carried out by a select committee of professionals in evaluating the performance of other professionals in meeting the standards of their specialty. Review by peers is used by editors in the evaluation of articles and other papers submitted for publication. Peer review is used also in the evaluation of grant applications. It is applied also in evaluating the quality of health care provided to patients.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Proteins obtained from ESCHERICHIA COLI.
Infections with bacteria of the species ESCHERICHIA COLI.
A verocytotoxin-producing serogroup belonging to the O subfamily of Escherichia coli which has been shown to cause severe food-borne disease. A strain from this serogroup, serotype H7, which produces SHIGA TOXINS, has been linked to human disease outbreaks resulting from contamination of foods by E. coli O157 from bovine origin.
Proteins found in any species of bacterium.
The functional hereditary units of BACTERIA.

Coexpression, purification and characterization of the E and S subunits of coenzyme B(12) and B(6) dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli. (1/6)

D-Ornithine aminomutase from Clostridium sticklandii comprises two strongly associating subunits, OraS and OraE, with molecular masses of 12,800 and 82,900 Da. Previous studies have shown that in Escherichia coli the recombinant OraS protein is synthesized in the soluble form and OraE as inclusion bodies. Refolding experiments also indicate that the interactions between OraS and OraE and the binding of either pyridoxal phosphate (PLP) or adenosylcobalamin (AdoCbl) play important roles in the refolding process. In this study, the DNA fragment containing both genes was cloned into the same expression vector and coexpression of the oraE and oraS genes was carried out in E. coli. The solubility of the coexpressed OraS and OraE increases with decreasing isopropyl thio-beta-D-galactoside induction temperature. Among substrate analogues tested, only 2,4-diamino-n-butyric acid displays competitive inhibition of the enzyme with a K(i) of 96 +/- 14 microm. Lys629 is responsible for the binding of PLP. The apparent K(d) for coenzyme B(6) binding to d-ornithine aminomutase is 224 +/- 41 nm as measured by equilibrium dialysis. The mutant protein, OraSE-K629M, is successfully expressed. It is catalytically inactive and unable to bind PLP. Because no coenzyme is involved in protein folding during in vivo translation of OraSE-K629M in E. coli, in vitro refolding of the enzyme employs a different folding mechanism. In both cases, the association of the S and E subunit is important for D-ornithine aminomutase to maintain an active conformation.  (+info)

A locking mechanism preventing radical damage in the absence of substrate, as revealed by the x-ray structure of lysine 5,6-aminomutase. (2/6)

Lysine 5,6-aminomutase is an adenosylcobalamin and pyridoxal-5'-phosphate-dependent enzyme that catalyzes a 1,2 rearrangement of the terminal amino group of dl-lysine and of l-beta-lysine. We have solved the x-ray structure of a substrate-free form of lysine-5,6-aminomutase from Clostridium sticklandii. In this structure, a Rossmann domain covalently binds pyridoxal-5'-phosphate by means of lysine 144 and positions it into the putative active site of a neighboring triosephosphate isomerase barrel domain, while simultaneously positioning the other cofactor, adenosylcobalamin, approximately 25 A from the active site. In this mode of pyridoxal-5'-phosphate binding, the cofactor acts as an anchor, tethering the separate polypeptide chain of the Rossmann domain to the triosephosphate isomerase barrel domain. Upon substrate binding and transaldimination of the lysine-144 linkage, the Rossmann domain would be free to rotate and bring adenosylcobalamin, pyridoxal-5'-phosphate, and substrate into proximity. Thus, the structure embodies a locking mechanism to keep the adenosylcobalamin out of the active site and prevent radical generation in the absence of substrate.  (+info)

The bacterial YbaK protein is a Cys-tRNAPro and Cys-tRNA Cys deacylase. (3/6)

Bacterial prolyl-tRNA synthetases and some smaller paralogs, YbaK and ProX, can hydrolyze misacylated Cys-tRNA Pro or Ala-tRNA Pro. To assess the significance of this quality control editing reaction in vivo, we tested Escherichia coli ybaK for its ability to suppress the E. coli thymidylate synthase thyA:146CCA missense mutant strain, which requires Cys-tRNA(Pro) for growth in the absence of thymine. Missense suppression was observed in a ybaK deletion background, suggesting that YbaK functions as a Cys-tRNA Pro deacylase in vivo. In vitro studies with the full set of 20 E. coli aminoacyl-tRNAs revealed that the Haemophilus influenzae and E. coli YbaK proteins are moderately general aminoacyl-tRNA deacylases that preferentially hydrolyze Cys-tRNA Pro and Cys-tRNA Cys and are also weak deacylases that cleave Gly-tRNA, Ala-tRNA, Ser-tRNA, Pro-tRNA, and Met-tRNA. The ProX protein acted as an aminoacyl-tRNA deacylase that cleaves preferentially Ala-tRNA and Gly-tRNA. The potential of H. influenzae YbaK to hydrolyze in vivo correctly charged Cys-tRNA Cys was tested in E. coli strain X2913 (ybaK+). Overexpression of H. influenzae ybaK decreased the in vivo ratio of Cys-tRNA Cys to tRNA Cys from 65 to 35% and reduced the growth rate of strain X2913 by 30% in LB medium. These data suggest that YbaK-mediated hydrolysis of aminoacyl-tRNA can influence cell growth.  (+info)

Mechanism of radical-based catalysis in the reaction catalyzed by adenosylcobalamin-dependent ornithine 4,5-aminomutase. (4/6)

 (+info)

Large-scale domain dynamics and adenosylcobalamin reorientation orchestrate radical catalysis in ornithine 4,5-aminomutase. (5/6)

 (+info)

Clostridium sticklandii, a specialist in amino acid degradation:revisiting its metabolism through its genome sequence. (6/6)

 (+info)

The article reviews recent investigations on the following aspects of protein metabolism in muscle 1 In muscle the average rates of protein catabolism vary under different physiological conditions, e.g. fasting. Several factors have been found to reduce protein catabolism, including insulin, glucose, and branched chain amino acids. In addition, repeated contractions and passive tension on the muscle retard net proteolysis. 2 Muscle rapidly oxidizes 3 required amino acids - leucine, isoleucine, and valine. This process increases severalfold upon fasting, in diabetes, and after hypophysectomy. 3 Muscle releases into the circulation large amounts of alanine. This process is linked to degradation of the branched chain amino acids. Since alanine is a major precursor for glucose synthesis in liver and since liver releases branched chain amino acids selectively during gluconeogenesis, there appears to be a cycle between muscle and liver of branched chain amino acids and alanine.
Amino acids play a central role in plant growth and development, as well as human and animal nutrition. In addition to serving as the building blocks for proteins, amino acids are involved in essential biological processes that include nitrogen assimilation, specialized metabolism, osmotic adjustment, alternative energy, and signaling (Rai 2002; Araújo et al. 2010; Angelovici et al. 2010, 2011; Wu and Messing 2014). Therefore, it is no surprise that the homeostasis for absolute levels and relative composition of the free amino acid (FAA) pool is complex and depends, at least in part, on various factors such as allosteric regulation, feedback loops of key metabolic enzymes in amino acid synthesis pathways, and the rate of amino acid degradation (Less and Galili 2008; Jander and Joshi 2010; Hildebrandt et al. 2015; Huang and Jander 2017; Amir et al. 2018). Studies have also demonstrated that core metabolism has a significant impact on FAA homeostasis. For example, alteration of the ...
Unit I. Protein structure and function. Amino acids ; Structure of proteins ; Globular proteins ; Fibrous proteins ; Enzymes -- Unit II. Bioenergetics and carbohydrate metabolism. Bioenergetics and oxidative phosphorylation ; Introduction to carbohydrates ; Introduction to metabolism and glycolysis ; Tricarboxylic acid cycle and pyruvate dehydrogenase complex ; Gluconeogenesis ; Glycogen metabolism ; Metabolism of monosaccharides and disaccharides ; Pentose phosphate pathway and nicotinamide adenine dinucleitide phosphate ; Glycosaminoglycans, proteoglycans, and glycoproteins -- Unit III. Lipid metabolism. Dietary lipid metabolism ; Fatty acid, ketone body, and triacylglycerol metabolism ; Phospholipid, glycosphingolipid, and eicosanoid metabolism -- Unit IV. Nitrogen metabolism. Amino acids : Disposal of nitrogen ; Amino acid degradation and synthesis ; Conversion of amino acids to specialized products ; Nucleotide metabolism -- Unit V. Integration of metabolism. Metabolic effects of insulin ...
Results High FPG mothers had a metabolic profile consistent with insulin resistance including higher triglycerides, 3-hydroxybutyrate, and amino acids including alanine, proline and branched chain amino acids (false discovery rate (FDR)-adjusted p,0.05). Lower 1,5-anhydroglucitol in high FPG mothers suggested recent hyperglycemic excursions (FDR-adjusted p,0.05). Pathway analyses indicated differences in amino acid degradation pathways for the two groups (FDR-adjusted p,0.05), consistent with population-based findings in non-pregnant populations. Exploratory analyses with newborn outcomes indicated positive associations for maternal triglycerides with neonatal sum of skinfolds and cord C-peptide and a negative association between maternal glycine and cord C-peptide (p,0.05). ...
Drostanolone (INN; also known as dromostanolone or Drolban), marketed as Masteron, is an anabolic steroid. Drostanolone is a part of thedihydrotestosterone (DHT) family. Its main medical uses include lowering cholesterol levels and as an antineoplastic agent in the treatment of some cancers. It is most commonly marketed as the ester drostanolone propionate (trade name Masteron).. Drostanolone binds to a receptor and activates a cascade of genetic changes, which increases the protein synthesis (anabolism) and decreases the amino acids degradation (catabolism). Other effects of Drostanolone are reduction or inhibition of prolactin or estrogen receptors, which are linked to its anti tumor properties. ...
TestAmerica Sacramento implemented the TOP assay as a solution to this complex problem. The TOP assay rapidly converts polyfluorinated PFAA precursors into PFAAs including PFOA, using a hydroxyl radical-based chemical oxidation method. The TOP assay replicates what micro-organisms in the environment would achieve after many years. The end result is to provide a range of PFAAs which are detectable by LCMSMS[1]. The TOP assay quantifies the sum of PFAS that could be converted to PFAAs in the environment. The TOP methodology has revealed that for AFFF-impacted sites, the existing analytical LCMSMS methods are only detecting an estimated 30% to 50% of the total PFAA mass present as PFAA precursors. ...
Business search by category in Waterproof 71375,Louisiana, United States. ZIPCode2Business allows you to find a business in over 800 business categories.
Kanjee at al. solved the Lcd1 x-ray strucutre, and the protein turned out to be in complex with ppGpp. Enzymatic assays showed that ppGpp inhibits Lcd1, thus providing a link between the acid and stringent responses: these two are in a sense are mutually exclusive. During the stringent response ppGpp alters RNA Polymerase in such a way that genes involved in amino acid biosynthesis are predominantly transcribed, and during the acid stress response Lcd1 degrades lysine. Inhibition of Ldl1 by ppGpp stops amino acid degradation, shifting the balance even more towards the amino acid production ...
Histidylhistidine is a dipeptide composed of two histidine residues. It is an incomplete breakdown product of protein digestion or protein catabolism. Dipeptides are organic compounds containing a sequence of exactly two alpha-amino acids joined by a peptide bond. Some dipeptides are known to have physiological or cell-signalling effects although most are simply short-lived intermediates on their way to specific amino acid degradation pathways following further proteolysis ...
Ribosomally synthesized and post-translationally modified peptides (RiPPs) are a novel class of natural products including several antibiotics and bacterial toxins. In countless RiPP biosynthetic pathways, cobalamin-dependent radical SAM (B12/rSAM) enzymes play a pivotal role. In the biosynthetic pathway of the antibiotic and anti-cancer agent thiostrepton A, TsrM, a B12/rSAM enzyme, catalyses the transfer of a methyl group to an electrophilic carbon atom of tryptophan. Here we show that methylcob(III)alamin is the probable physiological enzyme cofactor, and cob(II)alamin rather than cob(I)alamin is a key reaction intermediate. Furthermore, we establish that TsrM and a triple-alanine mutant alkylate cob(II)alamin efficiently leading to the synthesis of MeCbl. Exploiting TsrM substrate ambiguity, we demonstrate that TsrM does not catalyse substrate H-atom abstraction like most radical SAM enzymes. Based on these data, we propose an unprecedented radical-based C-methylation mechanism, which further
Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.
Allergy Research Group features B12 Adenosylcobalamin a highly absorbable form of vitamin B12. Vitamin B12 plays a role in brain and nervous system health. Also features folic...
Mihaela Testfamily: Neue Serie von Wellness & Beauty: Taufrisch belebt wird meins! #neubeirossmann #Rossmann #wellnessandbeauty #Lemongras #Beauty #Peeling #Spa
I am considering the addition of adenosylcobalamin to my supplements. Which brand do you use? I want one without folic acid/folate added. Thanks!
Interpreting these chemical reactions, it can be observed that sodium hypochlorite acts as an organic and fat solvent degrading fatty acids, transforming them into fatty acid salts (soap) and glycerol (alcohol), that reduces the surface tension of the remaining solution (scheme 1 - saponification reaction).. Sodium hypochlorite neutralizes amino acids forming water and salt (scheme 2 - neutralization reaction). With the exit of hydroxyl ions, there is a reduction of pH. Hypochlorous acid, a substance present in sodium hypochlorite solution, when in contact with organic tissue acts as a solvent, releases chlorine that, combined with the protein amino group, forms chloramines (scheme 3 - chloramination reaction). Hypochlorous acid (HOCl-) and hypochlorite ions (OCl-) lead to amino acid degradation and hydrolysis.. The chloramination reaction between chlorine and the amino group (NH) forms chloramines that interfere in cell metabolism. Chlorine (strong oxidant) presents antimicrobial action ...
A 1973 study suggests that once absorbed, MeCbl may be retained in the body better than CNCbl (1).. A 2011 clinical trial from Korea found that 1,500 µg/day of MeCbl was effective at raising vitamin B12 levels, reducing or eliminating neurological symptoms of B12 deficiency, and lowering homocysteine levels (5). This trial was done on people who had a gastrectomy and, therefore, had vitamin B12 malabsorption, indicating that for most people 1,500 µg/day would be more than enough. There was no comparison group receiving CNCbl.. I am unaware of any clinical trials testing the various forms of vitamin B12 against each other among the general population and most people seem to do well using CNCbl.. Some people with chronic fatigue report getting more relief from AdoCbl than either MeCbl or CNCbl (more info), while other people report feeling better only when taking both AdoCbl and MeCbl. Its possible this could be a real effect, but could also be due to a placebo effect or taking more B12 and ...
Reactome is pathway database which provides intuitive bioinformatics tools for the visualisation, interpretation and analysis of pathway knowledge.
Purpose: To evaluate the in vitro anti-oxidant and anti-inflammatory potential of the most potent and abundant metabolite, 3-methoxy quercetin (3-MQ), from extract fractions of mistletoe, Loranthus micranthus Linn (Loranthaceae) parasitic on Kola acuminata Schott & Endl, (Malvaceae), also known as Garcinia kola Heckel, (Clusiaceae).. Methods: Compounds isolated through a combination of chromatographic techniques were screened for in vitro antioxidant potential using the diphenyl picrazyl hydrazine (DPPH) radical-based model. Cell viability at 1-1000 μM 3-MQ in 24 h was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MTT) test. Lipopolysaccharide (LPS), phorbol 12-myristate 13-acetate (n = 5) five (5) replicates.. Results: Ten (10) known compounds including 3-MQ (1) were isolated and characterized. 3-MQ exhibited highly significant (p , 0.05) antioxidant activity with 50 % inhibitory concentration (IC50) of 15.0 μM; concentrations ≤ 100 μM did not exert ...
The Rossmann fold is a protein structural motif found in proteins that bind nucleotides, especially the cofactor NAD. The structure is composed of three or more parallel beta strands linked by two alpha helices in the topological order beta-alpha-beta-alpha-beta. Because each Rossmann fold can bind one nucleotide, binding domains for dinucleotides such as NAD consist of two paired Rossmann folds that each bind one nucleotide moiety of the cofactor molecule. Single Rossmann folds can bind mononucleotides such as the cofactor FMN. The motif is named for Michael Rossmann who first pointed out that this was a frequently occurring motif in nucleotide binding proteins.[1] ...
Adenosylcobalamin Decreased Symptom Checker: Possible causes include Methylmalonic Acidemia with Homocystinuria Type cblJ. Check the full list of possible causes and conditions now! Talk to our Chatbot to narrow down your search.
Vitamin B12 may be a useful vehicle for delivering diagnostic and therapeutic agents to various malignancies. Further evaluation of cobalamin analogs and their interaction with transport proteins and cellular receptors within malignant tissue and infection is warranted.
Stadtman, T. C.; McClung, L. S. (1957). "Clostridium Sticklandii Nov. Spec". Journal of Bacteriology. 73 (2): 218-219. doi: ... McClung, L. S.; Toabe, R. (1947). "The Egg Yolk Plate Reaction for the Presumptive Diagnosis of Clostridium sporogenes and ... CS1 maint: discouraged parameter (link) McClung, L. S. (1945). "Human Food Poisoning Due to Growth of Clostridium perfringens ( ...
Tsuda Y, Friedmann HC (1970). "Ornithine metabolism by Clostridium sticklandii. Oxidation of ornithine to 2-amino-4- ... Stadtman TC (1973). "Lysine metabolism by clostridia. XIIB 2,4-Diaminohexanoate dehydrogenase (2,4-diaminopentanoate ...
Chen HP, Lin CF, Lee YJ, Tsay SS, Wu SH (2000). "Purification and properties of ornithine racemase from Clostridium sticklandii ...
V. Some properties of the cobamide coenzyme-dependent beta-lysine mutase of Clostridium sticklandii". Arch. Biochem. Biophys. ...
Purification and properties of D-proline reductase and a proline racemase from Clostridium sticklandii". The Journal of ... of proline racemase was first put forward in the late sixties by Cardinale and Abeles using the Clostridium sticklandii enzyme ... Novel proline racemases of medical and veterinary importance were described respectively in Clostridium difficile (Q17ZY4) and ...
Purification and properties of a D-proline reductase and a proline racemase from Clostridium sticklandii". J. Biol. Chem. 228: ... "Identification of D-proline reductase from Clostridium sticklandii as a selenoenzyme and indications for a catalytically active ...
In the early 1950s, 5,6-LAM was discovered in the amino-acid-fermenting bacteria Clostridium sticklandii, in which lysine ... of an adenosine triphosphate regulated B 12-coenzyme-dependent D-alpha-lysine mutase complex from Clostridium sticklandii". ... and formate metabolism in an amino acid fermenting Clostridium". Journal of Bacteriology. 67 (6): 651-7. doi:10.1128/JB.67.6. ...
... of the proproteins GrdE of protein B of glycine reductase and PrdA of D-proline reductase from Clostridium sticklandii: ...
... thermolacticum Clostridium sticklandii Clostridium straminisolvens Clostridium subterminale Clostridium sufflavum Clostridium ... Clostridium aceticum Clostridium acetireducens Clostridium acetobutylicum Clostridium acidisoli Clostridium aciditolerans ... Clostridium acidurici Clostridium aerotolerans Clostridium aestuarii Clostridium akagii Clostridium aldenense Clostridium ... difficile Clostridium diolis Clostridium disporicum Clostridium drakei Clostridium durum Clostridium estertheticum Clostridium ...
Clostridium sticklandii, and Clostridium aminophilum sp. nov". International Journal of Systematic Bacteriology. 43 (1): 107- ... LPSN "Clostridium aminophilum" at the Encyclopedia of Life Type strain of Clostridium aminophilum at BacDive - the Bacterial ... Clostridium aminophilum is a species of gram-positive ammonia-producing ruminal bacteria, with type strain FT. Paster, B. J.; ... Houlihan AJ, Russell JB (2003). "The susceptibility of ionophore-resistant Clostridium aminophilum F to other antibiotics". J ...
... shares the highest number of genes that are homologous with Clostridium difficile (a pathogenic species ... sticklandii. One such selenoprotein, glycine reductase A was first identified in Cl. sticklandii. C. sticklandii uses a total ... Clostridium sticklandii is an anaerobic, motile, gram-positive bacterium. It was first isolated in 1954 from the black mud of ... Clostridium sticklandii has a genome that consists of one circular chromosome. On this chromosome are 2,715,461 base pairs. Of ...
Clostridium sticklandii MeSH B03.300.390.400.200.713 - Clostridium symbiosum MeSH B03.300.390.400.200.722 - Clostridium tertium ... Clostridium sticklandii MeSH B03.510.415.400.200.713 - Clostridium symbiosum MeSH B03.510.415.400.200.722 - Clostridium tertium ... Clostridium botulinum type G MeSH B03.300.390.400.200.180 - Clostridium butyricum MeSH B03.300.390.400.200.200 - Clostridium ... Clostridium botulinum type G MeSH B03.510.415.400.200.180 - Clostridium butyricum MeSH B03.510.415.400.200.200 - Clostridium ...
Clostridium sticklandii shares the highest number of genes that are homologous with Clostridium difficile (a pathogenic species ... sticklandii. One such selenoprotein, glycine reductase A was first identified in Cl. sticklandii. C. sticklandii uses a total ... Clostridium sticklandii is an anaerobic, motile, gram-positive bacterium. It was first isolated in 1954 from the black mud of ... Clostridium sticklandii has a genome that consists of one circular chromosome. On this chromosome are 2,715,461 base pairs. Of ...
Stadtman, T. C.; McClung, L. S. (1957). "Clostridium Sticklandii Nov. Spec". Journal of Bacteriology. 73 (2): 218-219. doi: ... McClung, L. S.; Toabe, R. (1947). "The Egg Yolk Plate Reaction for the Presumptive Diagnosis of Clostridium sporogenes and ... CS1 maint: discouraged parameter (link) McClung, L. S. (1945). "Human Food Poisoning Due to Growth of Clostridium perfringens ( ...
C. sticklandii possesses both the F-type and V-type ATPases. The discovery of an as yet unrecognized selenoprotein in the D- ... C. sticklandii is one of the best biochemically studied proteolytic clostridial species. Useful additional information has been ... With the goal of revisiting its carbon, nitrogen, and energy metabolism, and comparing studies with other clostridia, its ... Besides, experimental procedures reveal that C. sticklandii degrades amino acids in a preferential and sequential way. The ...
Among them, Clostridium sticklandii is one of the potential organisms to be exploited as a microbial cell factory for biofuel ... Genome-scale modeling and simulations have been guided for metabolic engineering of Clostridium species for the production of ... Clostridium sticklandii genome. C. sticklandii is a Gram-positive, anaerobic, motile and non-pathogenic bacterium isolated from ... 2 Genome-scale metabolic information of Clostridium sticklandii DSM 519 and related Clostridium species, collected from the ...
Proline biosynthesis from L-ornithine in Clostridium sticklandii: purification of δ1-pyrroline-5-carboxylate reductase, and ...
REMARK 999 ALIGNMENT BETWEEN THE ORAE PROTEIN SEQUENCES FROM CLOSTRIDIUM REMARK 999 STICKLANDII AND CLOSTRIDIUM DIFFICILE ( ... WE DO NOT FIND ANY EVIDENCE FOR THESE REMARK 999 RESIDUES FROM CLOSTRIDIUM STICKLANDII GENOMIC DNA SEQUENCING OR BY ... CLOSTRIDIUM STICKLANDII; SOURCE 3 ORGANISM_TAXID: 1511; SOURCE 4 GENE: ORAE; SOURCE 5 EXPRESSION_SYSTEM: ESCHERICHIA COLI; ... CLOSTRIDIUM STICKLANDII; SOURCE 12 ORGANISM_TAXID: 1511; SOURCE 13 GENE: ORAS; SOURCE 14 EXPRESSION_SYSTEM: ESCHERICHIA COLI; ...
Acetoanaerobium sticklandii DSM 519 Taxonomy ID : 499177. Lineage: cellular organisms; Bacteria; Terrabacteria group; ... Firmicutes; Clostridia; Clostridiales; Peptostreptococcaceae; Acetoanaerobium; Acetoanaerobium sticklandii. Glycoside Hydrolase ...
Fermentation of ornithine by Clostridium sticklandii.. PubMed: Taste stimuli: a behavioral categorization.. ...
Ornithine degradation in Clostridium sticklandii; pyridoxal phosphate and coenzyme A dependent thiolytic cleavage of 2-amino-4- ... The enzyme, characterized from the bacterium Clostridium sticklandii, is part of a degradation pathway of ornithine. It is ...
Clostridium sticklandii, a specialist in amino acid degradation: revisiting its metabolism through its genome sequence. BMC ... Other bacteria, such as Clostridium sticklandii, which also metabolize multiple amino acids as carbon and energy sources, show ... Kinetic parameters and cytotoxic activity of recombinant methionine γ-lyase from Clostridium tetani, Clostridium sporogenes, ... sticklandii (44), and may also help conserve energy in P. gingivalis (46). Five proteins from this respiratory chain were more ...
sticklandii and Cl. villosum. These specificities are based on neutralization studies. Other Clostridium species that can be ... Specialist Clostridium species that can be detected and/or whose nucleic acid can be isolated include but are not limited to Cl ... Species of Clostridium that can be detected and/or whose nucleic acid can be isolated include psychrophilic, mesophilic or ... Proteolytic species of Clostridium that can be detected and/or whose nucleic acid can be isolated include but are not limited ...
Clostridium sticklandii and Peptostreptococcus anaerobius were found to be the most sensitive species, while Streptococcous ...
V. Some properties of the cobamide coenzyme-dependent β-lysine mutase of Clostridium sticklandii. Arch. Biochem. Biophys. 125 ( ... 6-aminomutase from Clostridium sticklandii. J. Biol. Chem. 275 (2000) 106-114. [PMID: 10617592]. 6. Tang, K.H., Harms, A. and ... properties of an adenosine triphosphate regulated B12-coenzyme-dependent D-α-lysine mutase complex from Clostridium sticklandii ...
Recombinant Clostridium sticklandii 3-aminobutyryl-CoA ammonia lyase (kal). Product Gene Name ...
Clostridium sticklandii belongs to a cluster of non-pathogenic proteolytic clostridia which utilize amino acids as carbon and ... Clostridium sticklandii, a specialist in amino acid degradation:revisiting its metabolism through its genome sequence. ... It consists of at least three similar peptides active against Clostridium perfringens. In this article, a 15-kb region from R. ... With the goal of revisiting its carbon, nitrogen, and energy metabolism, and comparing studies with other clostridia, its ...
Clostridium sticklandii, a specialist in amino acid degradation: revisiting its metabolism through its genome sequence. BMC ... Australia-home of Clostridium difficile clade 5?, abstr P53. Abstr 4th Int Clostridium difficile Symp, Bled, Slovenia. ... Clostridium difficile Infection. Clostridium difficile is a spore-forming, Gram-positive, anaerobic bacillus found ubiquitously ... In 2013, further taxonomic revision of the Clostridia was called for, with a proposed name change from Clostridium to ...
Clostridium tetranomorpkum Ciostridium barkeri L-P-lysine aminomutase Clostridium sticklandii (I) (11) ca. 60000 [a] Km(Rbm), ... 60000 160000 Km(Rb"),MgZm(Mn2"). ATP, pyridoxal phosphate Ornithine aminomutase Clostridium sticklandii (I) ca. 60000 160000 ... by Clostridium ther51. The net reactions which occur are summarized rno~ceticum[~ in eqs. (e) to (g)[261. [email protected] 8 H + + 2 HzO 2 ... sticklandii catalyze the reversible conversion of a-lysine into P-lysine. Analogous isomerizations were observed with arginine ...
Clostridium botulinum type F (proteolytic), C. ghoni, C. mangenoti and C. putrificum were found to reduce proline to 5- ... C. botulinum type G and C. subterminale used glycine, lysine, serine, and arginine but in contrast to C. sticklandii they ... The method has been used to study the amino acids metabolized by cultures of proteolytic clostridia growing in a medium ... aminovaleric acid and to produce 2-aminobutyric acid, properties they shared with C. sporogenes and C. sticklandii. ...
Kenland) phenolic compounds and their screening for activity against Clostridium sticklandii, a hyper ammonia-producing ...
1999) Identification of d‐proline reductase from Clostridium sticklandii as a selenoenzyme and indications for a catalytically ...
... difficile strains but are absent from other clostridia. This may be explained by very high heterogeneity of the Clostridium ... sticklandii. Two antisense RNAs, CD630_n00090 and SQ771, are found in C. beijerinckii, and in C. botulinum, C. ljungdahlii, C. ... In Clostridium acetobutylicum, we have recently described a novel mechanism of control of a sulfur metabolic operon by an ... Clostridium difficile is an emergent pathogen, and the most common cause of nosocomial diarrhea. In an effort to understand the ...
... dependent Clostridium sticklandii D-ornithine aminomutase in Escherichia coli. Eur J Biochem. 2004;271:4293-7 pubmed ...
Clostridium sticklandii and C. valericum, gram-positive anaerobic bacteria belonging to the proteolytic clostridia, can produce ... Fermentation of delta-aminovaleric acid by Clostridium aminovalericum n. sp. J Bacteriol. 1960, 79: 549-552.PubMed Central ... Elsden SR, Hilton MG, Waller JM: The end products of the metabolism of aromatic amino acids by Clostridia. Arch Microbiol. 1976 ... Clostridium difficile and Lactobacillus strains are known to produce p-cresol by decarboxylation of p-hydroxyphenylacetate [32 ...
Identification of D-proline reductase from Clostridium sticklandii as a selenoenzyme and indications for a catalytically active ...
Stool testing for Clostridia is usually of limited usefulness since most Clostridia species are considered probiotics or ... sticklandii, C. lituseburense, C. subterminale, C.putifaciens, C. propionicum, C. malenomenatum, C.limosum, C. lentoputrescens ... The biochemical role of Clostridia in altering brain neurotransmitters is due to the fact that Clostridia metabolites ... William Shaw to be due to a combination of human metabolism and the metabolism by a group of Clostridia species, including but ...
A probe based on the sequence of the gene encoding selenoprotein A of glycine reductase of Clostridium sticklandii was used to ... The selenoprotein A component of the glycine reductase complex of Clostridium sticklandii was shown to differ in certain… (More ... The complete amino acid sequence of Clostridium sticklandii selenoprotein A, a selenocysteine-containing protein component of ... properties and biological activities of selenoprotein A components isolated from Clostridium sticklandii and Clostridium… ...
CLOSTRIDIUM STICKLANDII --. 3KP0_A E3PY95 ORAE. ESCHERICHIA COLI. CLOSTRIDIUM STICKLANDII --. 3KP0_E E3PY96 ORAS. ESCHERICHIA ... CLOSTRIDIUM STICKLANDII --. 3KP0_G E3PY96 ORAS. ESCHERICHIA COLI. CLOSTRIDIUM STICKLANDII --. 3KP0_F E3PY96 ORAS. ESCHERICHIA ... CLOSTRIDIUM STICKLANDII --. 3KP0_D E3PY95 ORAE. ESCHERICHIA COLI. CLOSTRIDIUM STICKLANDII --. 3KP0_H E3PY96 ORAS. ESCHERICHIA ...
Clostridium sticklandii (organism). Code System Preferred Concept Name. Clostridium sticklandii (organism). Concept Status. ...
CLOSTRIDIUM STICKLANDII --. 3KOX_B E3PY95 ORAE. ESCHERICHIA COLI. CLOSTRIDIUM STICKLANDII --. 3KOX_C E3PY95 ORAE. ESCHERICHIA ... CLOSTRIDIUM STICKLANDII --. 3KOX_H E3PY96 ORAS. ESCHERICHIA COLI. CLOSTRIDIUM STICKLANDII --. 3KOX_F E3PY96 ORAS. ESCHERICHIA ... CLOSTRIDIUM STICKLANDII --. 3KOX_A E3PY95 ORAE. ESCHERICHIA COLI. CLOSTRIDIUM STICKLANDII --. 3KOX_D E3PY95 ORAE. ESCHERICHIA ...
  • Clostridium botulinum type F (proteolytic), C. ghoni, C. mangenoti and C. putrificum were found to reduce proline to 5-aminovaleric acid and to produce 2-aminobutyric acid, properties they shared with C. sporogenes and C. sticklandii. (nih.gov)
  • The following species were eliminated: Gram(+) anaerobes: Clostridium sporogenes, Eubacterium saburreum, and Lactobacillus fermentum, and Gram(-) anaerobes: Bacteroides ovatus, Bacteroides uniformis, Fusobacterium mortiferum, and Prevotella oralis, as well as Gram(+) aerobic bacterium Staphylococcus aureus MSSA. (thefreedictionary.com)
  • Clostridium sticklandii has a genome that consists of one circular chromosome. (wikipedia.org)
  • With the goal of revisiting its carbon, nitrogen, and energy metabolism, and comparing studies with other clostridia, its genome has been sequenced and analyzed. (biomedcentral.com)
  • Analysis of the C. sticklandii genome and additional experimental procedures have improved our understanding of anaerobic amino acid degradation. (biomedcentral.com)
  • Genome-scale modeling and simulations have been guided for metabolic engineering of Clostridium species for the production of organic solvents and organic acids. (cyberleninka.org)
  • Clostridium sticklandii shares the highest number of genes that are homologous with Clostridium difficile (a pathogenic species of Clostridia') and with two members of the genus Alkaliphilus. (wikipedia.org)
  • Clostridium difficile infection (CDI) is the leading cause of antimicrobial and health care-associated diarrhea in humans, presenting a significant burden to global health care systems. (pubmedcentralcanada.ca)
  • C. difficile is taxonomically distinct from many other well-known clostridia, with a diverse population structure comprising hundreds of strain types spread across at least 6 phylogenetic clades. (pubmedcentralcanada.ca)
  • Clostridium difficile is a spore-forming, Gram-positive, anaerobic bacillus found ubiquitously in the environment and the gastrointestinal tracts of humans and animals. (pubmedcentralcanada.ca)
  • Clostridium difficile is an emergent pathogen, and the most common cause of nosocomial diarrhea. (prolekare.cz)
  • Certain amino acids, and cysteine in particular, promptly blocked toxin expression in Clostridium difficile strain VPI 10463 when added to late-exponential-phase peptone-yeast cultures, i.e. prior to normal induction of toxins A and B. Glucose reduced toxin yields by 80-fold, but only when supplemented at inoculation. (microbiologyresearch.org)
  • Repression of Clostridium difficile toxin gene expression by CodY. (microbiologyresearch.org)
  • Regulated transcription of Clostridium difficile toxin genes. (microbiologyresearch.org)
  • Effect of isoleucine on toxin production by Clostridium difficile in a defined medium. (microbiologyresearch.org)
  • C. sticklandii is one of the best biochemically studied proteolytic clostridial species. (biomedcentral.com)
  • Comparative genomics has provided the opportunity to study the lifestyle of pathogenic and non-pathogenic clostridial species as well as to elucidate the difference in metabolic features between clostridia and other anaerobes. (biomedcentral.com)
  • In this context, one of the best biochemically studied clostridial species is Clostridium sticklandii . (biomedcentral.com)
  • 1982), this species is an earlier heterotypic synonym of Clostridium perenne (Prévot 1940) McClung and McCoy 1957 (Approved Lists 1980) . (dsmz.de)
  • Clostridium sticklandii is an anaerobic, motile, gram-positive bacterium. (wikipedia.org)
  • Clostridium sticklandii belongs to a cluster of non-pathogenic proteolytic clostridia which utilize amino acids as carbon and energy sources. (biomedcentral.com)
  • The method has been used to study the amino acids metabolized by cultures of proteolytic clostridia growing in a medium consisting of an acid hydrolysate of casein as a source of amino acids and small amounts of yeast extract and trypticase as sources of growth factors. (nih.gov)
  • General Information: This Clostridium (HF strain, ATCC 12662) was initially found associated with Methanococcus vannieli in formate-enriched cultures. (up.ac.za)
  • C. botulinum type G and C. subterminale used glycine, lysine, serine, and arginine but in contrast to C. sticklandii they neither reduced proline to 5-aminovaleric acid nor produced 2-aminobutyric acid. (nih.gov)
  • Clostridium botulinum E3 str. (up.ac.za)
  • Clostridium botulinum B str. (up.ac.za)
  • sticklandii have two carbon dioxide fixation pathways, Wood-Ljundgahl and Glycine synthase/glycine reductase pathways. (wikipedia.org)
  • C. sticklandii harbours both the glycine synthase/glycine reductase and the Wood-Ljungdahl pathways. (biomedcentral.com)
  • Selenoprotein A component of the glycine reductase complex from Clostridium purinolyticum: nucleotide sequence of the gene shows that selenocysteine is encoded by UGA. (semanticscholar.org)
  • The gene encoding the selenoprotein A component of glycine reductase was isolated from Clostridium purinolyticum. (semanticscholar.org)
  • The enzyme, characterized from the bacterium Clostridium sticklandii, is part of a degradation pathway of ornithine. (kegg.jp)
  • The Stickland reaction is the basic bioenergetic pathway of some bacteria of the genus Clostridium . (biomedcentral.com)
  • sticklandii has genes that allow for the bacterium to be cultivated in aerobic conditions. (wikipedia.org)
  • Clostridium sticklandii is a Gram-positive bacterium with low (G +C) content that is capable of fermenting amino acids. (up.ac.za)
  • 16] The RA patients had significantly less bifidobacteria and bacteria of the Bacteroides-Porphyromonas- Prevotella group, Bacteroides fragilis subgroup, and Eubacterium rectale-Clostridium coccoides group Toivanen et al. (thefreedictionary.com)
  • A striking metabolic feature of Clostridia is the fermentation of amino acids via the Stickland reaction, described in 1934 by L. H. Stickland [ 7 ]. (biomedcentral.com)
  • sticklandii ferment amino acids by using the Stickland reaction, which couples the oxidation of one amino acid and the reduction of another. (wikipedia.org)
  • sticklandii has the ability to utilize both pathways, it has not been determined if they do utilize both pathways at the same time. (wikipedia.org)
  • later subjective synonyms of Clostridium barati . (dsmz.de)
  • Among them, Clostridium sticklandii is one of the potential organisms to be exploited as a microbial cell factory for biofuel production. (cyberleninka.org)
  • Biochemical studies have shown that C. sticklandii obtains energy for growth from oxidation-reduction reactions between specific amino acid pairs. (up.ac.za)