Clostridium chauvoei: A species of gram-positive bacteria in the family Clostridiaceae isolated from infected CATTLE; SHEEP; and other animals. It causes blackleg in cattle and sheep and is transmitted through soil-borne spores.Clostridium: A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.Clostridium Infections: Infections with bacteria of the genus CLOSTRIDIUM.Clostridium septicum: A species of gram-positive bacteria in the family Clostridiaceae. Infections have a strong association with malignancies and also with GAS GANGRENE.Clostridium difficile: A common inhabitant of the colon flora in human infants and sometimes in adults. It produces a toxin that causes pseudomembranous enterocolitis (ENTEROCOLITIS, PSEUDOMEMBRANOUS) in patients receiving antibiotic therapy.RNA, Ribosomal, 16S: Constituent of 30S subunit prokaryotic ribosomes containing 1600 nucleotides and 21 proteins. 16S rRNA is involved in initiation of polypeptide synthesis.Clostridium botulinum: A species of anaerobic, gram-positive, rod-shaped bacteria in the family Clostridiaceae that produces proteins with characteristic neurotoxicity. It is the etiologic agent of BOTULISM in humans, wild fowl, HORSES; and CATTLE. Seven subtypes (sometimes called antigenic types, or strains) exist, each producing a different botulinum toxin (BOTULINUM TOXINS). The organism and its spores are widely distributed in nature.AxisTeaching Materials: Instructional materials used in teaching.Pharmacology, Clinical: The branch of pharmacology that deals directly with the effectiveness and safety of drugs in humans.Competitive Behavior: The direct struggle between individuals for environmental necessities or for a common goal.Agriculture: The science, art or practice of cultivating soil, producing crops, and raising livestock.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Video Games: A form of interactive entertainment in which the player controls electronically generated images that appear on a video display screen. This includes video games played in the home on special machines or home computers, and those played in arcades.Food Inspection: Examination of foods to assure wholesome and clean products free from unsafe microbes or chemical contamination, natural or added deleterious substances, and decomposition during production, processing, packaging, etc.Anthrax: An acute infection caused by the spore-forming bacteria BACILLUS ANTHRACIS. It commonly affects hoofed animals such as sheep and goats. Infection in humans often involves the skin (cutaneous anthrax), the lungs (inhalation anthrax), or the gastrointestinal tract. Anthrax is not contagious and can be treated with antibiotics.Bacillus anthracis: A species of bacteria that causes ANTHRAX in humans and animals.Manuals as Topic: Books designed to give factual information or instructions.Meat: The edible portions of any animal used for food including domestic mammals (the major ones being cattle, swine, and sheep) along with poultry, fish, shellfish, and game.Tolonium Chloride: A phenothiazine that has been used as a hemostatic, a biological stain, and a dye for wool and silk. Tolonium chloride has also been used as a diagnostic aid for oral and gastric neoplasms and in the identification of the parathyroid gland in thyroid surgery.Burial: The act or ceremony of putting a corpse into the ground or a vault, or into the sea; or the inurnment of CREMAINS.Staphylococcus: A genus of gram-positive, facultatively anaerobic, coccoid bacteria. Its organisms occur singly, in pairs, and in tetrads and characteristically divide in more than one plane to form irregular clusters. Natural populations of Staphylococcus are found on the skin and mucous membranes of warm-blooded animals. Some species are opportunistic pathogens of humans and animals.Serratia liquefaciens: A species of gram-negative bacteria in the genus SERRATIA found in plants and the DIGESTIVE TRACT of rodents. It is the most prevalent Serratia species in the natural environment.Serratia: A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria that occurs in the natural environment (soil, water, and plant surfaces) or as an opportunistic human pathogen.Staphylococcus aureus: Potentially pathogenic bacteria found in nasal membranes, skin, hair follicles, and perineum of warm-blooded animals. They may cause a wide range of infections and intoxications.Sarcocystis: A genus of protozoa found in reptiles, birds, and mammals, including humans. This heteroxenous parasite produces muscle cysts in intermediate hosts such as domestic herbivores (cattle, sheep, pigs) and rodents. Final hosts are predators such as dogs, cats, and man.Staphylococcus hominis: A species of STAPHYLOCOCCUS similar to STAPHYLOCOCCUS HAEMOLYTICUS, but containing different esterases. The subspecies Staphylococcus hominis novobiosepticus is highly virulent and novobiocin resistant.Shewanella putrefaciens: A species of SHEWANELLA noted for its ability to reduce iron and manganese anaerobically.Protoplasts: The protoplasm and plasma membrane of plant, fungal, bacterial or archaeon cells without the CELL WALL.Listeria monocytogenes: A species of gram-positive, rod-shaped bacteria widely distributed in nature. It has been isolated from sewage, soil, silage, and from feces of healthy animals and man. Infection with this bacterium leads to encephalitis, meningitis, endocarditis, and abortion.Cell Wall: The outermost layer of a cell in most PLANTS; BACTERIA; FUNGI; and ALGAE. The cell wall is usually a rigid structure that lies external to the CELL MEMBRANE, and provides a protective barrier against physical or chemical agents.Muramidase: A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.Schizophyllum: A genus of fleshy shelf basidiomycetous fungi, family Schizophyllaceae, order POLYPORALES, growing on woody substrata. It is pathogenic in humans.Lipase: An enzyme of the hydrolase class that catalyzes the reaction of triacylglycerol and water to yield diacylglycerol and a fatty acid anion. It is produced by glands on the tongue and by the pancreas and initiates the digestion of dietary fats. (From Dorland, 27th ed) EC 3.1.1.3.Listeriosis: Infections with bacteria of the genus LISTERIA.Mastitis, Bovine: INFLAMMATION of the UDDER in cows.Mastitis: INFLAMMATION of the BREAST, or MAMMARY GLAND.Staphylococcal Infections: Infections with bacteria of the genus STAPHYLOCOCCUS.Coagulase: Enzymes that cause coagulation in plasma by forming a complex with human PROTHROMBIN. Coagulases are produced by certain STAPHYLOCOCCUS and YERSINIA PESTIS. Staphylococci produce two types of coagulase: Staphylocoagulase, a free coagulase that produces true clotting of plasma, and Staphylococcal clumping factor, a bound coagulase in the cell wall that induces clumping of cells in the presence of fibrinogen.Bacteriological Techniques: Techniques used in studying bacteria.Milk: The white liquid secreted by the mammary glands. It contains proteins, sugar, lipids, vitamins, and minerals.Vaccines, Conjugate: Semisynthetic vaccines consisting of polysaccharide antigens from microorganisms attached to protein carrier molecules. The carrier protein is recognized by macrophages and T-cells thus enhancing immunity. Conjugate vaccines induce antibody formation in people not responsive to polysaccharide alone, induce higher levels of antibody, and show a booster response on repeated injection.Polysaccharides, Bacterial: Polysaccharides found in bacteria and in capsules thereof.Neisseria meningitidis: A species of gram-negative, aerobic BACTERIA. It is a commensal and pathogen only of humans, and can be carried asymptomatically in the NASOPHARYNX. When found in cerebrospinal fluid it is the causative agent of cerebrospinal meningitis (MENINGITIS, MENINGOCOCCAL). It is also found in venereal discharges and blood. There are at least 13 serogroups based on antigenic differences in the capsular polysaccharides; the ones causing most meningitis infections being A, B, C, Y, and W-135. Each serogroup can be further classified by serotype, serosubtype, and immunotype.Haemophilus influenzae: A species of HAEMOPHILUS found on the mucous membranes of humans and a variety of animals. The species is further divided into biotypes I through VIII.Blood-Testis Barrier: A specialized barrier, in the TESTIS, between the interstitial BLOOD compartment and the adluminal compartment of the SEMINIFEROUS TUBULES. The barrier is formed by layers of cells from the VASCULAR ENDOTHELIUM of the capillary BLOOD VESSELS, to the SEMINIFEROUS EPITHELIUM of the seminiferous tubules. TIGHT JUNCTIONS form between adjacent SERTOLI CELLS, as well as between the ENDOTHELIAL CELLS.Streptococcus pneumoniae: A gram-positive organism found in the upper respiratory tract, inflammatory exudates, and various body fluids of normal and/or diseased humans and, rarely, domestic animals.Harm Reduction: The application of methods designed to reduce the risk of harm associated with certain behaviors without reduction in frequency of those behaviors. The risk-associated behaviors include ongoing and active addictive behaviors.UkraineAIDS Serodiagnosis: Immunologic tests for identification of HIV (HTLV-III/LAV) antibodies. They include assays for HIV SEROPOSITIVITY and HIV SERONEGATIVITY that have been developed for screening persons carrying the viral antibody from patients with overt symptoms of AIDS or AIDS-RELATED COMPLEX.HIV Infections: Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS).Needle-Exchange Programs: Organized services for exchange of sterile needles and syringes used for injections as a potential means of reducing the transmission of infectious diseases.Substance Abuse, Intravenous: Abuse, overuse, or misuse of a substance by its injection into a vein.Pakistan

Human fulminant gas gangrene caused by Clostridium chauvoei. (1/5)

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Detection and identification by PCR of Clostridium chauvoei in clinical isolates, bovine faeces and substrates from biogas plant. (2/5)

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Novel real-time PCR assay for simultaneous detection and differentiation of Clostridium chauvoei and Clostridium septicum in clostridial myonecrosis. (3/5)

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Genetic and functional characterization of the NanA sialidase from Clostridium chauvoei. (4/5)

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The utilization of a commercial soil nucleic acid extraction kit and PCR for the detection of Clostridium tetanus and Clostridium chauvoei on farms after flooding in Taiwan. (5/5)

Clostridial diseases are zoonoses and are classified as soil-borne diseases. Clostridium chauvoei and Clostridium tetani cause blackleg disease and tetanus, respectively. Since bacteria and spores are re-distributed by floods and then, subsequently, contaminate soils, pastures and water; the case numbers associated with clostridial diseases usually increase after floods. Because Taiwan is often affected by flood damage during the typhoon season, possible threats from these diseases are present. Thus, this study's aim is to apply a combination of a commercial nucleic acid extraction kit and PCR to assess the prevalence of Clostridia spp. in soil and to compare the positivity rates for farms before and after floods. The minimum amounts of Clostridium tetanus and Clostridium chauvoei that could be extracted from soils and detected by PCR were 10 and 50 colony forming units (cfu), respectively. In total, 76 samples were collected from the central and southern regions of Taiwan, which are the areas that are most frequently damaged by typhoons. Noteworthy, the positive rates for Clostridium tetanus and Clostridium chauvoei in Pingtung county after the severe floods caused by a typhoon increased significantly from 13.73 and 7.84% to 53.85 and 50.00%, respectively. This study for the first time provides the evidence from surveillance data that there are changes in the environmental distribution of Clostridium spp. after floods. This study indicates that screening for soil-related zoonotic pathogens is a potential strategy that may help to control these diseases.  (+info)

*Clostridium chauvoei

"Clostridium chauvoei". Catalogue of Life. ITIS. Species 2000. Clostridium chauvoei at the Encyclopedia of Life Clostridium ... Clostridium chauvoei is an anaerobic, motile, Gram-positive bacterium. In cattle and sheep, a severe infection causes blackleg ... A study conducted in Taiwan sought to test this correlation by using nucleic acid to extract Clostridium chauvoei from the soil ... For the first time, there is evidence to show that the environmental distribution of Clostridium chauvoei is increased after ...

*Raouf Salama Moussa

Moussa, R. S. (1958). "Complexity of Toxins from Clostridium Septicum and Clostridium Chauvoei". Journal of Bacteriology. 76 (5 ...

*Auguste Chauveau

The bacterial species Clostridium chauvoei is named after him. He was the author of Traité d'anatomie comparée des animaux ...

*Common eland

Clostridium chauvoei, another bacterium, can be harmful as well. Eland are also hosts to several kinds of ticks. In one study ...

*Clostridium novyi

April 2008). "Human Fulminant Gas Gangrene Caused by Clostridium chauvoei". Journal of Clinical Microbiology. 46 (4): 1545-7. ... Some authors include Clostridium haemolyticum as Clostridium novyi type D. C novyi is closely related to Clostridium botulinum ... Clostridium novyi (oedematiens) a Gram-positive, endospore- forming, obligate anaerobic bacteria of the class clostridia. It is ... "Clostridia in Anti-tumour Therapy". In Brüggemann, Holger; Gottschalk, Gerhard. Clostridia: Molecular Biology in the Post- ...

*Blackleg (disease)

... is an infectious bacterial disease most commonly caused by Clostridium chauvoei, a Gram-positive bacterial species. It is seen ... Human Fulminant Gas Gangrene Caused by Clostridium chauvoei. J Clin Microbiol.; 46(4): 1545-1547. Irsik, M.B. Blackleg in ... C. chauvoei can produce a large amount of gas as a metabolic byproduct when growing and reproducing, hence the alternate name ... Gas gangrene Clostridium vaccine Mackintosh C, Haigh JC, Griffin F. Bacterial diseases of farmed deer and bison. Rev Sci Tech. ...

*Strikebreaker

... as in the blackleg infectious bacterial disease of sheep and cattle caused by Clostridium chauvoei. He dates the first use of ...

*List of MeSH codes (B03)

Clostridium cellulovorans MeSH B03.300.390.400.200.215 --- Clostridium chauvoei MeSH B03.300.390.400.200.250 --- clostridium ... Clostridium cellulovorans MeSH B03.510.415.400.200.215 --- Clostridium chauvoei MeSH B03.510.415.400.200.250 --- Clostridium ... Clostridium symbiosum MeSH B03.300.390.400.200.722 --- Clostridium tertium MeSH B03.300.390.400.200.725 --- Clostridium tetani ... Clostridium symbiosum MeSH B03.510.415.400.200.722 --- Clostridium tertium MeSH B03.510.415.400.200.725 --- Clostridium tetani ...
In order to get basic results which allow the phylogenetic localisation and identification of Clostridium chauvoei and the closely related species Clostridium septicum, we cloned and sequenced the rrs genes encoding the 16SrRNA for these species and determined their phylogenetic position in Clostridium cluster I (C. carnis, C. perfringens, C. botulinum, C. tetani). Based on DNA sequence data, we developed an identification system for C. chauvoei, using specific PCR amplification of the rrs gene with specific oligonucleotids. ... The developed identification system was evaluated on clinical material during a recent outbreak of blackleg. Thereby C. chauvoei was identified as the etiological agent of the outbreak either directly from clinical samples of muscle, liver spleen and kidney or from primary cultures made with this material ...
Fast Shipping. Great Low Price. 7-way blackleg plus red water protection for cattle and sheep. For prevention of Clostridium chauvoei, septicum, novyi, sordellii, perfringens Types C & D, plus haemolyticum (bacillary hemoglobinuria/red water). Give cattle and sheep 2 ml SQ. Repeat in 3-4 weeks and once annually.Vision-8 with Spur Cattle Sheep Vaccine Merck Blackleg New Adjuvant | Blackleg 7 8 Way | Cattle Va
Blackleg definition, Also called black quarter, symptomatic anthrax. Veterinary Pathology. an infectious, often fatal disease of cattle and sheep, caused by the soil bacterium Clostridium chauvoei and characterized by painful, gaseous swellings in the muscles, usually of the upper parts of the legs. See more.
For use in healthy cattle and calves three months of age or older as an aid in preventing blackleg caused by Clostridium chauvoei, malignant edema caused by Cl. septicum, black disease caused by Cl. novyi, gas-gangrene caused by Cl. sordellii, enterotoxemia and enteritis caused by Cl. perfringens Types B, C and D, and disease caused by Histophilus somni (Haemophilus somnus). Although Cl. perfringens Type B is not a significant problem in North America, immunity is provided by the beta toxoid of Type C and the epsilon toxoid of Type D. Ultrabac 7/Somubac consists of killed, standardized cultures of Cl. chauvoei, Cl. septicum, Cl. novyi, Cl. sordellii, Cl. perfringens Types C and D, and H. somni, with an adjuvant. Available in a 10 dose or 50 dose.. ...
For use in healthy cattle and sheep as an aid in preventing disease caused by Clostridium chauvoei (Blackleg), septicum (Malignant edema), haemolyticum (Bacilliary Hemoglobinuria (Red Water)), novyl (Black disease), sordellii and perfringens Types C&D (Enterotoxemia).
Black Quarter, Blackleg, quarter evil, or quarter ill is an infectious bacterial disease most commonly caused by Clostridium chauvoei, a Gram-positive bacterial species. It is seen in livestock all over the world, usually affecting cattle, sheep, and goats. It has been seen occasionally in farmed bison and deer. The acute nature of the disease makes successful treatment […]. Read More ». ...
For the vaccination of healthy cattle and sheep against diseases caused by Clostridium chauvoei Cl.septicum Cl.novyi Type B Cl.haemolyticum (known elsewhere as Cl. novyi Type D) Cl.tetani and Cl. perfringens Types C and D.
One Shot Ultra 8 Cattle Vaccine aids in the prevention of blackleg, malignant edema, bacilary hemoglobinuria, black disease, enterotoxemia and enteritis, and bovine pneumonia.
Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).. ⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!. ⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). ⇒ Compatible with the following automated extraction systems:. ...
Rapid magnetic bead nucleic acid extraction kit for use with any matrix or pathogen (DNA or RNA).. ⇒ The fastest magnetic bead extraction kit on the market, with results in only 20 min!. ⇒ Use in combination with the ID Gene™ range of amplification kits to obtain results in only 70 minutes for DNA and 90 minutes for RNA (extraction and amplification). ⇒ Compatible with the following automated extraction systems:. ...
... This report analyzes the worldwide markets for Biogas Plants in US$ Million. The report provides separate comprehensive analytics for the US, Canada, - Market research report and industry analysis - 12399232
The blackleg bacterium survives poorly in soil. All evidence suggests that the blackleg bacterium does not survive very well outside of association with host plant tissue. Hence, the seed tuber is the most important source of inoculum in the blackleg disease cycle. Removal of blackleg affected plants including below-ground portions is the best option. Take steps to prevent contact of diseased tissue with other plants in the field. It is unfortunate that there is no effective chemical control to manage this disease. The first and the most effective line of defense is the use pathogen-free tubers for seed. Warming of seed tubers to about 55°F before planting also helps. Seed treatment with fungicides do not directly affect these bacterial pathogens, but fungicides can reduce invasion by other fungi and therefore reduce opportunistic infection by Erwinia spp ...
nucleic acid extraction and purification Manufacturers & nucleic acid extraction and purification Suppliers Directory - choose nucleic acid extraction and purification from 306 nucleic acid extraction and purification Super Suppliers of China.
Biogas plants produce nutrient rich digestates as side products, which are usually used as local fertilisers. Yet the large amount and regional gradients of biogas plants in Germany necessitate management, conditioning, and transportation of digestates, in order to follow good fertilising procedure and prohibit local over-fertilisation. With a membrane-based treatment chain, i.e. centrifugation, ultrafiltration, and reverse osmosis, digestates can be separated into a solid N,P-fertiliser, a liquid N,K-fertiliser, and dischargeable water. Up to now, the high energy demand of the process chain, in particular the ultrafiltration step, limits the economical market launch of the treatment chain. A reduction of the energy demand is challenging, as digestates exhibit a high fouling potential and ultrafiltration fluxes differ considerably for digestates from different biogas plants. In a systematic screening of 28 digestate samples from agricultural biogas plants and 6 samples from bio-waste biogas ...
The Milan-based Life Science Division of Voden Medical Instruments S.p.A. has been awarded DiaSorin Platinum Distributor status in recognition of their outstanding contribution to sales of the DiaSorin Arrow instrument and molecular extraction kits throughout Italy. Meeting the specific needs of HLA (human leukocyte antigen) testing laboratories, assessing tissue compatibility in organ transplant patients, the Arrow Blood DNA extraction kit has shown particular growth and completes the Voden pro
When shopping with Valley Vet Supply, you will find our prices among the lowest anywhere on Blackleg Original Adjuvant. Paying less means better profit margins for the farm & ranch. Order now or request a free Farm & Ranch supply catalog.
Blackleg is a fatal disease of young cattle. It produces an acute local infection, and the resulting blood poisoning leads to rapid death.. The name blackleg derives from the fact that the site of infection is often a leg muscle, and that the affected muscle is dark in colour. This Primefact discusses:. ...
[vote] The best Gel extraction kit - posted in Molecular Cloning: I tried Roche, promega, Bioneer, Qiagen. I would say, Roche kit give the best yield how about you guy ?
Gentaur molecular products has all kinds of products like :search , BioBasic \ Rapid Animal Total RNA Extraction Kit \ AT4181 for more molecular products just contact us
The Presto™ DNA RNA Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA and total protein simultaneously from cultured cells, animal tissue, whole blood and biological fluids such as serum and plasma.
, Manufacturer of DNA RNA extraction products such gel extraction kits, pcr cleanup kits, rna kits, dna extraction kits, virus dna rna extraction kits.
Labtimes - News, information and analysis for the European Life Sciences: Rankings, Methods, Lab Tricks, Products, EU Funding, Jobs, Careers, Congresses, ...
Mec niques Segal s S.L we have specialised in slurry, digestates for biogas plants, the recovery of by-products and the contents of containers of swine liquid feed.
Schlüter, A., Bekel, T., Diaz, N.N., Dondrup, M., Eichenlaub, R., Gartemann, K.-H., Krahn, I., Krause, L., Kroemeke, H., Kruse, O., Mussgnug, J.H., Neuweger, H., Niehaus, K., Pühler, A., Runte, K.J., Szczepanowski, R., Tauch, A., Tilker, A., Viehöver, P., Goesmann, A.: The metagenome of a biogas-producing microbial community of a production-scale biogas plant fermenter analysed by the 454-pyrosequencing technology. Journal of Biotechnology. 136, 77-90 (2008 ...
Fernandez C.M., Molto E., Gallardo N., del Arco A., Martinez C., Andres A., Ros M., Carrascosa J.M., Arribas C.. Two variants of the adipose hormone resistin are generated by alternative splicing in Wistar rats. Here we analyzed the expression of these resistin variants in 2 main visceral adipose depots, epididymal and retroperitoneal, as well as the resistin serum concentration during aging and food restriction. Total protein levels of resistin were also analyzed in extracts from both visceral adipose depots. Resistin variants show similar patterns of relative expression in visceral adipose tissues in 3-month-old rats, representing the short variant, s-resistin, which is 15% of the full-length transcript. However, only epididymal, but not retroperitoneal, fat pad shows a decrease in both messenger RNA and protein levels of resistin isoforms with aging. Food restriction decreases adiposity index in 8- and 24-month-old animals to values even lower than those of 3-month-old animals. Food ...
Anaerobic digestion trials can improve biogas plant performance. See our case study, making changes from feedstock mix to digestate processing equipment.
Process monitoring in biogas plants Bernhard Drosg Technical Brochure written by: Bernhard Drosg Copyright 2013 IEA Bioenergy. All rights reserved. First electronic edition produced in 2013 A catalogue
Researchers are frantically trying to buy some time in their fight against a devastating crop disease. Blackleg is a fungal disease that practically wiped out Australias canola industry in the 1970s. Department of Environment and Primary Industries researcher Joshua Koh says scientists hope understanding more about canolas cousins will help in their battle.
The Monarch DNA Gel Extraction Kit rapidly and reliably purifies up to 5 μg of concentrated, high-quality DNA from agarose gels. The kit utilizes a bind/wash/elute workflow with minimal incubation and spin times. The columns ensure zero buffer retention and no carryover of contaminants, enabling elution of sample in volumes as low as 6 μl. The buffers provided have been optimized, and do not require monitoring of pH. Unlike other kits, there is no need to add isopropanol to the melted agarose prior to loading on the column, saving you a step. Eluted DNA is ready for use in restriction digests, DNA sequencing, ligation and other enzymatic manipulations. Designed with sustainability in mind, Monarch kits use significantly less plastic and responsibly-sourced, recyclable packaging.
AccuPrepTM 5M Plasmid Extraction Kit은 magnetic nano bead와 MagListoTM 자성입자 분리장치 (별도 구매)를 이용하여 5분(mini), 10분(midi), 15분(maxi) 내에 배양된 박테리아 세포로부터 plasmid를 추출하는 획기적인 제품입니다. 자성 나노 입자를 이용해 빠른 시간 안에 세포 분쇄물과 plasmid를 분리 시키고, 마찬가지로 자성 나노 입자를 이용해 plasmid를 농축 및 정제하는 과정을 거치기 때문에 원심분리기를 사용하는 spin column 방법에 비해 빠르게 박테리아 세포로부터 plasmid를 분리 할 수 있습니다. 본 제품은 mini, midi, maxi prep을 위해 별도의 kit을 구매하지 않고 한가지의 kit을 이용해 모두 prep 할 수 있는 장점을 가지고 있으며, midi나 maxi prep을 위해 별도의 vacuum system이나 air pressure system을 구비 할 필요가 없습니다. 또한 gravity를 이용한 plasmid extraction 방법은 많은 시간이 걸린다는 단점이
Rna Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 938 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
AccuPrep® Plasmid Mini Extraction Kit for 96 well vacuum manifold 는 E.coli등의 세포 배양액으로부터 고순도의 plasmid DNA를 신속하게 분리, 정제할 수 있도록 개발된 제품입니다. 본 제품은 96 well plate와 vacuum manifold를 사용함으로써 매우 신속하게 고순도, 고수율의 Plasmid DNA를 추출할 수 있습니다. 또한, 본 제품은Qiagen, Axygen, Promega 등의 vacuum manifold에 호환이 가능합니다.
Lucigen strives to provide life scientists with the highest quality products and services for RNA/DNA amplification, cloning, next gen sequencing, and protein expression. Experience outstanding performance with time-saving convenience at an exceptional price.
|h3>Why choose QIAsymphony SP for nucleic acid extraction?|/h3> |br /> QIAsymphony SP provides fast, flexible high-quality nucleic acid purification for a wide range of downstream applications. |br /> |br /> Explore the links below to see how others have used QIAsymphony SP in their work.
When considering methods to extract nucleic acids (DNA and RNA) to be used in downstream applications, it is important to think about what the sample source will be, as well as if it will be able to be used in an eventual assay. Being able to extract and purify DNA can lead to important information…
Lucigen strives to provide life scientists with the highest quality products and services for RNA/DNA amplification, cloning, next gen sequencing, and protein expression. Experience outstanding performance with time-saving convenience at an exceptional price.
This technique allows for efficient, highly purified cytoplasmic and nuclear-associated compartment fractionation utilizing NP-40 detergent in mammalian cells. The nuclear membrane is not disturbed during the fractionation thus leaving all nuclear and perinuclear associated components in the nuclear fraction. This protocol has been modified from Sambrook and Russell (2001) in order to downscale the amount of cells needed. To determine the efficiency of fractionation, we recommend using qPCR to compare the subcellular compartments that have been purified with equivalent amount of control whole cell extracts.
* found in: gMax MINI Genomic DNA Kit, DNA Fragment Extraction Kit, Total RNA (Plant) Kit, The gMax MINI genomic DNA kit provides an efficient method for..
Choosing a DNA or RNA purification kit. Select your sample type, the type of nucleic acid you want to extract and the volume/throughput scale of purification. You will then be offered a range of DNA or RNA extraction kits that meet your specifications.
I am trying to extract RNA from eosinophils. Ive tried the Invitrogen Micro-to-Midi extraction kit, but didnt get any results. Does anyone have any advice or ideas on how to increase my yield ...
General questions about domestic biogas plant. 1) What types of waste can be treated ?. Any type of easily degradable waste such as cooked waste, flesh of meat, and fish, ripe fruits and vegetables and human excreta, urine etc.. 2) Is it possible to treat liquid waste in the same plant ?. Yes. The organic waste water generated in the kitchen such as waste water obtained while cleaning rice, fish and meat etc. can be treated. The waste containing chemical, soap and detergent should be avoided.. 3) What is the procedure for the waste collection ?. Two bin collection system can be used. It involves the use of two different bins to collect the degradable and non degradable wastes. Use a separate bin for carrying the waste to the plant.. 4) What is the procedure of waste feeding ?. Collect easily degradable waste mixed with the liquid waste from the kitchen and pour it in to the plant through inlet tank.. 5) How much quantity of waste can be treated ?. The quantity of waste to be treated depend upon ...
AccuPrep® GMO DNA Extraction Kit allows the extraction of DNA from agricultural products such as beans, corn, and rice or from processed foods such as bean sprouts, tofu and condensed milk, for the detection of GMOs. The kit includes all reagents required for extraction and in addition uses a column type extraction system to allow a more rapid, more convenient extraction of higher-quality DNA when compared to conventional methods. A high amount of high-purity DNA can be extracted; for example, 20 ~ 40 ug of DNA for 1 g of powdered bean, with an A260/A280 ratio of over ...
Diseases of Beef Cattle at Cattle Today Online! A listing of the common diseases of beef cattle including their symptoms, treatment and prevention.
Original issues were scanned as 400 dpi 8-bit greyscale tiffs on an Epson Expression 10000 XL scanner with Adobe Photoshop CS2. Color pages were scanned as 400 dpi 24-bit color tiffs on the same machine. Using Adobe Acrobat XI Pro, the tiffs were converted to pdfs and OCRed, then uploaded into CONTENTdm ...
Official site for Flax Council of Canada. The Flax Council of Canada is a national organization which promotes Canadian flax and flax products for nutritional and industrial uses in domestic and international markets.
◇실험의 목적◇ Agarose gel에서 원하는 DNA fragment를 분리 정제한다. ◇실험 방법◇ 1. DNA Loading 2. Gel cutting 3. Gel extraction 1) gel extraction buffer 500 ㎕, 60℃로 녹인다. 2) 4. DNA loading(확인용) ◇실험의 원리◇ -전기영동의 원리 다양한 방법의 전기영동법이 있지만 우리가 실험한 Agarose gel 전기영동은 DNA 조각을 분리하고 인식하는데 사용하는 가장 일반적인 방법이다. 이 기술은 간단하고 빠르며 다른 방식으로는 잘 ..
We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, well assume that you are happy to receive all cookies on the Greiner Bio-One website. However, if you would like to, you can change your cookie settings at any time ...
海词词典,最权威的学习词典,专业出版acetic acid extraction是什么意思,acetic acid extraction的用法,acetic acid extraction翻译和读音等详细讲解。海词词典:学习变容易,记忆很深刻。
This article describes the Tissue and Hair Extraction Kit (for use with DNA IQ™), which provides a simple and rapid approach to processing tissue and hair samples and leads seamlessly into the DNA IQ System protocol to purify DNA free of PCR inhibitors in a manual or automated format.
Privacy and Disclosure: New Jersey National Guard Family Programs respects your right to privacy; however, the staff members DO NOT have privileged communication. New Jersey State Family Programs Staff is required by law, with or without your consent, to contact proper authorities if they believe you intend to harm yourself or others or if family member abuse, child neglect or drug use is suspected.. ...
Downloadable! In this paper we investigate how changes in the support scheme may affect electricity generation from agricultural Combined Heat and Power (CHP) biogas plants in Germany. A recursive Multi-Agent System (MAS) for investment decision-making is coupled with GIS data. The spatial-temporal diffusion model accounts for the limited availability of substrate resources, alternative plant sizes and different heat use combinations. For illustration, we apply the model to the German federal states of North Rhine-Westphalia and Bavaria, for which we estimate an additional economic capacity potential of 409 MWel. Overall, we conclude that current feed-in payments per unit of electric power provided are probably not too far off the optimum level, if one considers the maximum diffusion of CHP units possible. However, the current feed-in system may overtly favor small generating units, thereby failing to incentivize coordination among farmers for joint resource use in larger and more efficient plants. In
Biogas is one of the most untapped sources of natural and sustainable energy available. It is used all over the world and by far the largest number of biogas plant (technically known as anaerobic digestion plants) are relatively small installations and are found in the developing nations. India for instance has more than 12 million digesters). The Africa Biogas Partnership Programme (ABPP) 70,000 biogas plants in Ethiopia, Kenya, Tanzania, Uganda, Senegal and Burkina Faso. That project alone will be expected to be providing about half a million people access to a sustainable source of energy by this year 2013. Biogas plants can be useful resources wherever you are. An example of this is the Mount Everest area. It is a destination for climbers and trekkers from all over the world. While visiting our worlds highest mountain, climbers, trekkers, and walkers take away great memories, lots of photos and new friends, but leave behind their untreated waste. At the Mount Everest Biogas Project they are ...
countries and other applications.. Through the biogas process, waste food and agricultural products are converted to natural gas through bacterial digestion. The BioSPR makes the fats, sugars, proteins and starches more accessible to the bacteria, which increases yield and can reduce viscosity. The Italian biogas market is one of the worlds largest with more than 700 plants.. "Three-Es offers their customers a beautifully engineered SPR solution that, as ...
Commercially available RNA extraction kits are rapid, capable of high-throughput analysis and cost-effective [1]. The isolation of DNA-free RNA is crucial to the success of highly sensitive assays like RT-PCR. RNA extraction procedures frequently result in RNA preparations that are highly contaminated with genomic DNA, which often leads to false-positive RT-PCR outcomes. The presence of DNA in an RNA sample can be detected easily by an appropriate PCR test of an indicator gene. Then, if necessary, treatment of the RNA preparation with DNase I will usually eliminate, or at least substantially reduce, the content of DNA [2].. The RNA concentration of a sample is commonly determined via measurement of absorbance at a wavelength of 260 nm (A260). The purity of the RNA sample can be determined using the A260/A280 ratio as a reference (a value of ~2.0 is considered "pure" RNA). However, the accuracy of this method is questionable, because protein contamination can cause an overestimation (,50%) of RNA ...
A feasibility assessment of a system integrating an ethanol manufacturing facility, a confined feedlot operation (CFO) and a biogas plant.
One Shot Ultra 7 - 50 ds Vial offers 7-way protection against blackleg and pasteurella. It also has a special adjuvant called Stimugen.
* found in: Rapid Fungal RNA Extraction Kit, EZ-10 Spin Column Plasmid DNA Mini-Preps Kits, RNase-Free DNase Set, Rapid Plant RNA Isolation Kit, EZ-10..
The invention relates to fungal disease resistance, in particular to resistance to blackleg disease caused by Leptosphaeria maculans. Provided are Brassica plants and seeds comprising a fragment of chromosome 8 of a wild B. rapa accession in their genome, wherein this fragment comprises a blackleg resistance locus. Further provided are molecular markers linked to the blackleg resistance locus and methods of using the markers. Brassica plants and seeds with stacked blackleg resistance loci are also provided.
Conference Comment: The contributor provides a good summary of C. piliforme, an atypical member of the genus Clostridium. Other members of the clostridia are large, Gram positive spore forming bacteria with straight or slightly curved morphology, in contrast the filamentous, Gram negative spore forming C. piliforme.(2) Further differentiating C. piliforme from other clostridia is the fact that it does not possess characteristics that allow its inclusion into one of the three general categories of the other pathogenic members of the genus. These categories of clostridia are neurotoxic (C. tetani, C. botulinum types A-G), histotoxic (C. chauvoei, C. septicum, C. novyi types A and B, C. perfringens type A, C. sordellii, C. hemolyticum), and enteropathogenic/enterotoxemia-producing (C. perfringens types A-E, C. difficile, C. colinum, C. spiroforme).(2 ...
Denator AB (Denator) is a biotechnology company. The company develops, markets and sells instruments and consumables to life science and clinical research markets. Its products include stabilizor system, stabilizor peptide extraction kit and stabilizor 2D-GE extraction kit. Denators stabilizor system is used for rapid conductive heating of preserve tissue sample. The companys stabilizol peptide extraction kit facilitates differentiation and identification of endogenous peptides from degradative peptide fragments. Its stabilizor 2D-GE extraction kit maximizes the recovery of proteins from heat-stabilized tissue to obtain a representative picture of protein states at the time of sampling. The company has research and applications facilities at the Uppsala Science Park, Sweden. Denator is headquartered in Gothenburg, Sweden.
The biogas production in Sweden year 2014 was 1,8 TWh where almost 1 TWh was upgraded to biomethane for use in vehicles. The production of biomethane is mainly located at the biogas plants that have a production over 200-400 Nm3/h. This is mainly due to the fact that the investment and operating costs becomes too high for the small producers with low biogas flows. The government of Sweden has a highly ambitious goal of having a vehicle fleet that runs on at least 80 % renewable fuels in the year 2030. To reach that goal even the small biogas producers needs to start upgrading their gas to be able to replace fossil fuels with renewable biomethane.. The aim of this master thesis has been to supply enough information to Norrmejerier about whether or not to upgrade the biogas by summarizing relevant research papers and calculate energy flows and costs for each upgrading technology.The limitation of the thesis has been that the production of biogas with different incoming substrates have been ...
For a class of 30 students or for 6 separate teacher demonstrations. Excellent for all levels of teaching. Demonstrates the DNA extraction process of freeze-dried E. coli cells. Cell walls are broken with a detergent, and the DNA is extracted onto a spooling rod. The exercise helps students visualiz...
Sending cow manure and coffee grinds to a new biogas plant is helping to protect the environment and maintain the purity of Switzerlands famous mineral water Henniez.
Browse a full line of products for small and large scale extraction and purification of nucleic acids. Select RNA-, DNA-, and PCR-specific kits and supplies.
Various methods of nucleic acid (NA) extraction were investigated with the aim of developing a quantitative method of NA extraction from five representative strains of biomining microorganisms. The process of removing cells from mineral surfaces, lysing microorganisms, precipitating NA and purifying RNA were analysed. The success of each method was examined spectrophotometrically, by agarose gel electrophoresis and PCR or quantitative real time PCR (qPCR). The most important step was shown to be cellular lysis, which principally impacted on thequantity of NA extracted from each strain. The quantity and quality of extracted NA was highlydependent on the method of NA precipitation. This study resulted in the development of a NA extraction method that reliably and reproducibly extracted NA from five strains of biomining microorganisms.. ...
Leucine-rich repeat receptor-like proteins (LRR-RLPs) are highly adaptable parts of the signalling apparatus for extracellular detection of plant pathogens. Resistance to blackleg disease of Brassica spp. caused by Leptosphaeria maculans is largely governed by host race-specific R-genes, including the LRR-RLP gene LepR3. The blackleg resistance gene Rlm2 was previously mapped to the same genetic interval as LepR3. In this study, the LepR3 locus of the Rlm2 Brassica napus line Glacier DH24287 was cloned, and B. napus transformants were analysed for recovery of the Rlm2 phenotype. Multiple B. napus, B. rapa and B. juncea lines were assessed for sequence variation at the locus. Rlm2 was found to be an allelic variant of the LepR3 LRR-RLP locus, conveying race-specific resistance to L. maculans isolates harbouring AvrLm2. Several defence-related LRR-RLPs have previously been shown to associate with the RLK SOBIR1 to facilitate defence signalling. Bimolecular fluorescence complementation (BiFC) and ...
Materials The gastric carcinoma BGC-823 cell line was purchased from Sunbio Company (Anyang, South Korea). RPMI 1640 and fetal bovine serum were purchased from Gibco Company (Langley, OK, USA). Transwell chamber and Martrigel were purchased from BD Bioscience Company (Franklin Lakes, New Jersey, USA). The lipidosome cell transfection kit was purchased from Invitrogen (Grand Island, NY, USA). The anti-P53 and anti-β-actin antibody were purchased from Xinlebio (Shanghai, China). CCK-8 detection kit was purchased from Beyotime Institute (Shanghai, China). Trizol reagents and cDNA synthesis kit were purchased from TAKARA (Japan). The restriction enzyme and T4 DNA ligase were purchased from NEB. The plasmid extraction kit was purchased from Omega Bio-tek, Inc (Norcross, GA, USA). The cell protein extraction kit was purchased from Thermo Scientific Company (Waltham, MA USA). SiRNA design and vector construction The TP53 siRNA sequence was designed using a publically available online software ...
Liebherr Machines Bulle SA develops and produces diesel and gas engines, injection systems, as well as hydraulic components and splitter boxes. The areas of application range from earthmoving and deep foundation machines, mining excavators, mobile and crawler cranes, maritime applications, material handling machines right up to biogas plants and combined heat and power units. Best quality and customized solutions for different requirements are the focal point.. ...
DNA and RNA extractions from soil without contamination by humics, polyphenols, or other PCR inhibitors can be quite challenging. Watch this video to find out how to isolate PCR-ready DNA from soil using a combination of the FastDNA™ Spin Kit for Soil and the FastPrep-24™ sample preparation instrument from MP Biomedicals. These enable complete lysis of all soil organisms including bacteria, fungi, and plant and animal cells, and isolation of pure DNA.
Miniprep plasmid DNA extraction kit is used when the starting E. coli culture volume is 1~5 ml of LB broth and the expected DNA yield is 20~30 μg. Other extraction and purification plasmid DNA Kits are available from varying manufacturers, named by size of bacterial culture, includes gigaprep, megaprep, and midiprep - Plasmid DNA Extraction (Miniprep) - AbVideo™ - Support - Abnova
The extraction and purification of genetic material from cells is often the first and most crucial step in life sciences research. Gram-positive bacteria, which contain thick cell walls, pose a challenge for efficient and effective nucleic acid extraction in this regard.. Dr. Vincent A. Fischetti and his research group have developed an effective enzyme-based nucleic acid extraction system. A group of enzymes isolated from viruses that infect specific bacteria (bacteriophages) was discovered as a potential antibacterial agent, and their mode of action is to compromise the integrity of the bacterial cell wall or membrane resulting in rapid lysis of the bacterial cell. Once the cell wall is removed, it is fairly straightforward to obtain the DNA or RNA for subsequent experiments, such as PCR, cloning, sequencing, and proteomics. The use of these enzymes as reagents for nucleic acid extraction shortens the time and increases the nucleic acid yield, which is greatly advantageous.. Several lysins ...
Based on our experience, the QIAquick PCR Purification Kit removes SYBR Green dye efficiently from PCR reactions.. We strongly assume that the QIAquick Gel Extraction Kit will be equally efficient in removing this dye, however, we recommend a 5 min incubation with wash Buffer PE on the QIAquick spin column at step 10 of the QIAquick Gel Extraction Kit Protocol. Efficiency of SYBR Green dye removal has to be validated by the enduser.. ...
Ludgate Environmental Fund, the AIM-listed company that invests in cleantech and environmental companies, has announced a €3m investment in agri.capital, a German developer, owner and operator of biogas plants ...
Despite financing hurdles, Tibbar Energy USVI is moving toward construction of its 7 MW biogas plant at the end of Q1 2015.The project will utilize Viaspace Inc.-licensed Giant King Grass, fats, oils, greases and food waste as feedstock. ...
Nucleic acid extraction and purification. DNA or RNA extraction is a crucial and necessary step in most molecular analyses for diagnostic, forensic or research purposes.. First, the starting sample needs to be lysed by mechanic, thermal and enzymic mechanisms; then, the nucleic acid is isolated and purified. The process requires extreme precision and performing it manually on a large scale can be a heavy task for the operator. Among the various nucleic acid extraction methods, the magnetic bead method is the quickest and easiest to automate. Magnetic beads are microspheres able to bind DNA or RNA by means of a special coating on surface.. Using this technology, OMNIA Prima workstation allows fully automated extraction of nucleic acids, processing up to 24 samples at a time. DNA or RNA can be isolated from biological fluids (whole blood, plasma, saliva), biopsy material even of few milligrams (fresh or frozen) and plant samples (leaves, shoots).. Prima is compatible with different commercial ...
Article Detection of parathion-methyl on gauze wipes. MATRIX: Cotton gauze pad (wipe) ANALYTE: Parathion-methyl RANGE OF DETECTION: 0.08 mg per wipe (100 cm2) MATERIALS: EnviroGard Parathion Plate Kit (item #75000). SDI Wipe Extraction Kit. Protec...
Definition of Clostridium septicum. Provided by Stedmans medical dictionary and Drugs.com. Includes medical terms and definitions.
San Francisco based Brice Frillici is a multimedia artist and performer who goes by the name of his unusual project "SEKDEK" (Spirit Extraction Kit, Demon Extraction Kit). It is an ongoing portrait series of himself, friends, and family members covered in colorful, psychedelic paint - and the result is fantastic and a little bit gory. Frillici describes his process as expressionistic painting by any means necessary; he spits, throws and spreads clay, acrylic paint, glitter, and flour onto his subjects, topping off their new personas with costume wigs, wild fabrics, and fake blood. One may not be surprised to find the elaborate and often graphic heavy metal band Gwar among his inspirations, as well as Bjork, the characters in Where the Wild Things Are, H. R. Giger illustrations, makeup design, and a variety of other visual references. It was French performance artist Olivier de Sagazan, whose work in the movie Samsara made the greatest impact on Frillici, where he violently applies clay, dots and ...
Ampliqon produces innovative solutions supplementing DNA and RNA extraction. We have recently launched a brand new product, named G2 DNA/RNA Enhancer. G2 DNA/RNA Enhancer increases the DNA yield from difficult samples such as clay dramatically. G2 DNA/RNA Enhancer must be used either in combination with commercial extraction kits or as part of the extraction method of interest.. ...
DaAn Gene is a high technology enterprise oriented in molecular diagnostic techniques. Since its establishing day in 1993, DaAn Gene has dedicated to the Molecular Diagnostics leader and consistently committed to R&D, production and service of Molecular Diagnostics. DaAn Gene has more than 20 years molecular diagnostic experience with multi-product lines like: 1. Real-time qPCR diagnostic kits and equipments 2. Automatic nucleic acid extraction 3. Pathology product 4. Immunoassay diagnostic kits. ...
We offer a variety of Affymetrix, Illumina, and Agilent microarray platforms and chemistries with fully automated sample preparation and processing. Comprehensive service offerings include nucleic acid extraction, analytical quality control, microarray preparation and analysis. We provide consultation for experimental design, platform and microarray selection, as well as Bioinformatics support.. ...
Chemicals, enzymes, and reagents. Restriction and modification enzymes and ligase were from New England Biolabs (Beverly, MA). TripleMaster Taq DNA polymerase was from Brinkmann Eppendorf (Hamburg, Germany). The plasmid pCMV-hRL from Promega (Madison, WI) containing a human codon optimized version of Renilla luciferase, was used as the template for the amplification of reporter gene fragments. The rapamycin-dimerizing proteins FRB and FKBP12 were obtained from the constructs pcDNA-Nrluc-FRB and pcDNA-FKBP12-Crluc from our previous study ( 13). Rapamycin was from Sigma (St. Louis, MO). LipofectAMINE transfection reagent was from Invitrogen (Carlsbad, CA). The plasmid extraction kit and DNA gel extraction kit were from Qiagen (Valencia, CA). Coelenterazine was from Nanolight (Pinetop, AZ). Bacterial culture media were from BD Diagnostic Systems (Sparks, MD). All cell culture media, fetal bovine serum (FBS), the antibiotics streptomycin, and penicillin, were from Invitrogen. Oligonucleotides were ...
This collaboration will combine enzymatic nucleic acid extraction technology with single-cell molecular tests for life science research and diagnostic applications.
The average home gardener is almost always better off buying fresh seed each season from a reliable source. However, saving seed can be a way of experimenting or preserving a particular variety. Problems in Saving Seed. There are several difficulties involved in saving seed from vegetables from your own garden. Among these are seed-borne diseases, immature seed, and unsatisfactory results from seed saved from hybrid and cross-pollinated plants. Certain diseases can be transmitted in seed. A disease that infects a crop by the end of the growing season may do little damage to that crop. However, if the seed is saved and planted the following year, the disease may severely injure or even kill the young plants. Examples of seed-borne diseases are black rot and blackleg of cabbage. Black rot causes a blackening of the veins, followed by stunting and decay of the head. It is caused by a bacterium that is often transmitted in the seed. Blackleg is a seed-borne fungus that kills the entire stems of the ...
0051]DHFR gene expression cassettes were prepared so as to place a DHFR gene under the control of a truncated promoter from which some GC-rich repeat sequences had been deleted, as follows. In order to amplify a DHFR gene, PCR was carried out using a pSV2-dhfr plasmid (ATCC No. 37146) with a pair of primers: dhfr 01 primer having a SmaI site (5-GCG CCC GGG ATG GTT CGA CCA TTG AAC TGC-3) and dhfr-02 timer having a BstBI site (5-CAC TTA GAA CCT GTT AGT CTT TCT TCT CGT AGA C-3). A PCR product of about 200 bp was electrophoresed on a 1% agarose gel and purified using a gel extraction kit (QIAGEN Cat No. 28706). The DNA fragment was cloned into a pDRIVE vector (Qiagen) and subjected to DNA sequencing. The DNA sequencing revealed that the amplified DHFR gene had no errors. Then, the pDRIVE-dhfr plasmid was digested with Smal and BstBI to excise the DHFR gene, and electrophoresed on a 1.5% agarose gel. A DHFR gene fragment of about 200 bp was purified using a gel extraction kit (QIAGEN Cat No. ...
By HELGE NEUMANN, RALF LOGES und FRIEDHELM TAUBE, Kiel. Due to the continuously increasing number of biogas plants in Germany, the area cultivated with silage maize Zea mays has been increasing significantly over the past few years. From the viewpoint of nature conservation it is generally supposed that this change in land use could result in losses of habitats of wild plant and animal species. In this paper we present the results of a study conducted in the federal state of Schleswig-Holstein in Northern Germany over the period 2005 to 2007. Paired comparisons of single arable fields were used to analyse whether silage maize fields were characterized by other bird communities than fields cultivated with common cash crops (winter cereals, winter oilseed rape). Birds were recorded during the breeding time as well as during the non-breeding time.. Maize fields and cash crop fields showed similar numbers of breeding bird species. Lapwings Vanellus vanellus occurred more often in maize fields, ...
0061] In addition to the rich solvent interface 19, the acid recovery apparatus 27 would comprise at least one acid extraction unit 14. The acid extraction unit 14 would comprise whatever equipment was necessary to effect the removal or recovery of at least one of the absorbed acid gas fractions from the rich solvent 9. In this particular case, two acid extraction units 14 are shown, to demonstrate the concept of the capture and/or removal of more than one acid gas fraction, by the conduct of more than one acid extraction step. Specifically, there is shown a first acid extraction unit 14 which would comprise whatever equipment or process was necessary to recover a first recovered acid gas fraction 15. The first recovered acid gas fraction 15 is in this case shown as well as a first recovered acid gas fraction discharge interface 20 which would basically be an interface to a storage tank or other apparatus into which the recovered acid gas fraction 15 would be conveyed. In this particular case ...
Today we inoculated - (continued growing) - the two colonies discovered on Friday. (They had not increased since then, so we are skeptical.) We pipetted 5mL of LB solution into 10mL tubes, added 5uL of ampicillin, and used pipette tips to scrape a bit out of each colony. The tips were dropped into the LB solution/ampicillin and the 10mL tubes were labeled and put into the shaker at 37-degrees Celsius overnight. The plates were kept at room temperature overnight ...
Unison Solutions, Inc. offers maintenance services for all of the equipment and systems we sell. Whether itís as straightforward as biogas testing and evaluation or as technical as a generator rebuild, Unison has the experience and expertise to meet your needs.

Clostridium chauvoei - WikipediaClostridium chauvoei - Wikipedia

"Clostridium chauvoei". Catalogue of Life. ITIS. Species 2000. Clostridium chauvoei at the Encyclopedia of Life Clostridium ... Clostridium chauvoei is an anaerobic, motile, Gram-positive bacterium. In cattle and sheep, a severe infection causes blackleg ... A study conducted in Taiwan sought to test this correlation by using nucleic acid to extract Clostridium chauvoei from the soil ... For the first time, there is evidence to show that the environmental distribution of Clostridium chauvoei is increased after ...
more infohttps://en.wikipedia.org/wiki/Clostridium_chauvoei

A biochemical study of the soluble antigen and haemolysin of clostridium chauvoeiA biochemical study of the soluble antigen and haemolysin of clostridium chauvoei

... Verpoorte, Jacob Arnold ... A biochemical study of the soluble antigen and haemolysin of clostridium chauvoei. Login ... chauvoei cultures had the character of proteins. This was revealed by the loss of activity when pepsin and trypsin were allowed ... chauvoei and Cl. septicum, a comparative chemical study of the toxins had not yet been carried out. A preliminary investigation ...
more infohttps://repository.up.ac.za/handle/2263/61727

Standards for Clostridium chauvoei vaccine the relationship between the response of guinea pigs and sheep following vaccination...Standards for Clostridium chauvoei vaccine the relationship between the response of guinea pigs and sheep following vaccination...

Twelve commercial 5-component clostridial vaccines with known variations in potency of the blackleg Clostridium chauvoei ... Standards for Clostridium chauvoei vaccine the relationship between the response of guinea pigs and sheep following vaccination ... Twelve commercial 5-component clostridial vaccines with known variations in potency of the blackleg (Clostridium chauvoei) ... and challenge with virulent Clostridium chauvoei. Crichton, R.; Harriss, D.A.; Mckay, D.J.. Australian Veterinary Journal 63(3 ...
more infohttps://gsajournals.org/articles/000/001/000001520-standards-for-clostridium-chauvoei-vaccine-the-relationship-between-the-response-of-guinea-pigs-and-sheep-following-vaccination-and-challeng.php

Ultrastructure of the Basal Organelles of Flagella of Clostridium chauvoei | Microbiology SocietyUltrastructure of the Basal Organelles of Flagella of Clostridium chauvoei | Microbiology Society

Ultrastructure of the Basal Organelles of Flagella of Clostridium chauvoei * R. C. HAMILTON ... Ultrastructure of the Basal Organelles of Flagella of Clostridium chauvoei, Page 1 of 1 ...
more infohttps://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-89-1-191

Genetic and functional characterization of the NanA sialidase from Clostridium chauvoei | Veterinary Research | Full TextGenetic and functional characterization of the NanA sialidase from Clostridium chauvoei | Veterinary Research | Full Text

The NanA protein is secreted by C. chauvoei as a dimer. The nanA gene was found to be conserved and sialidase activity was ... In this study we have analyzed the sialidase activity of the NanA protein of C. chauvoei and cloned the sialidase gene nanA. ... found in C. chauvoei strains isolated over a period of 50 years from various geographical locations. Antiserum directed against ... Clostridium chauvoei is the causative agent of blackleg, a wide spread serious infection of cattle and sheep with high ...
more infohttps://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-42-2

Search ResultsSearch Results

Clostridium chauvoei Clostridium chauvoei is a highly motile, Gram-positive, spore-forming bacillus. Toxins produced by the ... Clostridium chauvoei is the bacterial agent responsible for the acute febrile disease called blackleg, which infects skeletal ...
more infohttps://www.cabi.org/vetmedresource/search/?q=cattle&rows=10&sort=Relevance&facets=1&facet1f=Geographical+Location&facet1v=Chile&facet1v=Uruguay&facet1v=Peru&facet1v=Australia&facet1v=Iraq&facet1v=Ireland

Search ResultsSearch Results

Clostridium chauvoei Clostridium chauvoei is a highly motile, Gram-positive, spore-forming bacillus. Toxins produced by the ... Clostridium chauvoei is the bacterial agent responsible for the acute febrile disease called blackleg, which infects skeletal ...
more infohttps://www.cabi.org/vetmedresource/search/?q=cattle&rows=10&sort=Relevance&facets=1&facet1f=Geographical+Location&facet1v=Chile&facet1v=Uruguay&facet1v=Peru&facet1v=Australia&facet1v=Iraq&facet1v=Guatemala

export bact test 6 Flashcards by Agata Grzelak | Brainscapeexport bact test 6 Flashcards by Agata Grzelak | Brainscape

Clostridium chauvoei Animal: Yearling well-nourished steer in Missouri. Symptoms: sudden death. Lesions: Gas bubbles under skin ... How would Clostridium perfringens be classified in regards to utilization of oxygen in their environment? ... How would clostridium pefringens be classified in regards to utilization of oxygen in their environment? ... According to the required reading, what are the 3 most common clostridium involved in diarrhea diseases in baby pigs ...
more infohttps://www.brainscape.com/flashcards/export_bact-test-6-2475650/packs/4280740

Bactracillin G Benzathine for Animal Use - Drugs.comBactracillin G Benzathine for Animal Use - Drugs.com

3. Blackleg (Clostridium chauvoei).. Precautions. Exceeding the recommended doses and dosage levels may result in antibiotic ...
more infohttps://www.drugs.com/vet/bactracillin-g-benzathine.html

Free Agriculture Flashcards about Large Animal ReviewFree Agriculture Flashcards about Large Animal Review

Clostridium chauvoei. Clostridium novyi. Clostridium organisms. Clostridium perfringens. Clostridium perfringens. Clostridium ...
more infohttps://www.studystack.com/flashcard-1893373

Manual on meat inspection for developing countriesManual on meat inspection for developing countries

Clostridium chauvoei is also found in the digestive tract of healthy animals. In sheep the agent is transmitted through wounds ... It is caused by Clostridium chauvoei.. Transmission : The organisms of blackleg are found in the soil. During grazing, ... It is caused by Clostridium septicum and is manifested by wound infection. The infection is commonly soil-borne. Deep wounds ... Clostridia are soil-borne organisms which cause disease by releasing toxins. Specific antitoxin and antibiotics are rarely ...
more infohttp://www.fao.org/docrep/003/t0756e/T0756E03.htm

USDA APHIS | Supplemental Assay Methods - 200 SeriesUSDA APHIS | Supplemental Assay Methods - 200 Series

Potency Testing Products Containing Clostridium chauvoei Antigen. Dec 13, 2017. 201. Potency Testing Clostridium perfringens ... Potency Testing Clostridium sordellii Antigen. Dec 15, 2017. 213. Potency Testing of Clostridium botulinum Type C Bacterin- ... Potency Testing Clostridium perfringens Type C Beta Antitoxins. Dec 12, 2017. 203. Potency Testing Clostridium perfringens Type ... Potency Testing Clostridium novyi Type B Alpha Antigen. Dec 15, 2017. 209. Potency Testing Products Containing Clostridium ...
more infohttps://www.aphis.usda.gov/aphis/ourfocus/animalhealth/veterinary-biologics/biologics-regulations-and-guidance/sa_sams/ct_vb_sams_200_series

BenzaPen 48 - 500 mL  | QC SupplyBenzaPen 48 - 500 mL | QC Supply

Blackleg (Clostridium chauvoei). Benefits. *Fast acting - Noticeable improvement within 4 to 48 hours ...
more infohttps://www.qcsupply.com/benzapen-48-500ml.html

Chowdhury P[au] - PubMed - NCBIChowdhury P[au] - PubMed - NCBI

Corrigendum: Clostridium chauvoei, an Evolutionary Dead-End Pathogen.. Rychener L, In-Albon S, Djordjevic SP, Chowdhury PR, ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Chowdhury+P%5Bau%5D&dispmax=50

Common eland - WikipediaCommon eland - Wikipedia

Clostridium chauvoei, another bacterium, can be harmful as well. Eland are also hosts to several kinds of ticks. In one study ...
more infohttps://en.wikipedia.org/wiki/Common_Eland

Clostridium kluyveri | definition of Clostridium kluyveri by Medical dictionaryClostridium kluyveri | definition of Clostridium kluyveri by Medical dictionary

What is Clostridium kluyveri? Meaning of Clostridium kluyveri medical term. What does Clostridium kluyveri mean? ... Looking for online definition of Clostridium kluyveri in the Medical Dictionary? Clostridium kluyveri explanation free. ... Clostridium feseri. now called C. chauvoei (above).. Clostridium haemolyticum. formerly called C. novyi type D. See bacillary ... clostri´dia an individual of the genus Clostridium. clostrid´ial. clostridium. (klŏ-strĭd′ē-əm). n. pl. clostrid·ia (-ē-ə) Any ...
more infohttps://medical-dictionary.thefreedictionary.com/Clostridium+kluyveri

Clostridium haemolyticum | definition of Clostridium haemolyticum by Medical dictionaryClostridium haemolyticum | definition of Clostridium haemolyticum by Medical dictionary

What is Clostridium haemolyticum? Meaning of Clostridium haemolyticum medical term. What does Clostridium haemolyticum mean? ... Looking for online definition of Clostridium haemolyticum in the Medical Dictionary? Clostridium haemolyticum explanation free ... Clostridium feseri. now called C. chauvoei (above).. Clostridium haemolyticum. formerly called C. novyi type D. See bacillary ... Clostridium sporogenes. an apathogenic clostridium often found in lesions of gas gangrene. ...
more infohttps://medical-dictionary.thefreedictionary.com/Clostridium+haemolyticum

TCDB » SEARCHTCDB » SEARCH

CctA (Clostridium chauvoei toxin A; 317 aas) is the main cytotoxic and haemolytic substance secreted by C. chauvoei. ...
more infohttp://tcdb.org/search/result.php?tc=1.C.3.2

TCDB » SEARCHTCDB » SEARCH

CctA (Clostridium chauvoei toxin A; 317 aas) is the main cytotoxic and haemolytic substance secreted by C. chauvoei. ... NetF-producing type A Clostridium perfringens is an important cause of canine and foal necrotizing enteritis. NetF, related to ...
more infohttp://tcdb.org/search/result.php?tc=1.C.3.5

Frontiers | Current Concepts in the Management of Necrotizing Fasciitis | SurgeryFrontiers | Current Concepts in the Management of Necrotizing Fasciitis | Surgery

Human fulminant gas gangrene caused by Clostridium chauvoei. J Clin Microbiol (2008) 46:1545-7. doi:10.1128/JCM.01895-07 ... Type III includes monomicrobial infections involving the Clostridium species or Gram-negative bacteria. Clostridium species are ... Outbreak of necrotizing fasciitis due to Clostridium sordellii among black-tar heroin users. Clin Infect Dis (2004) 38:87-91. ... Type 3 NF should be managed with clindamycin and penicillin, which cover the Clostridium species. If Vibrio infection is ...
more infohttps://www.frontiersin.org/articles/10.3389/fsurg.2014.00036/full

Clostri Shield 7 (Canada) for Animal Use - Drugs.comClostri Shield 7 (Canada) for Animal Use - Drugs.com

Clostridium Chauvoei-Septicum-Novyi-Sordellii-Perfringens Types C & D Bacterin-Toxoid. For Use In Animals Only ... For use in healthy cattle and sheep as an aid in the prevention of diseases caused by C. chauvoei (blackleg), C. septicum ( ...
more infohttps://www.drugs.com/vet/clostri-shield-7-can.html

Goats (new with animal welfare information) | Infonet Biovision Home.Goats (new with animal welfare information) | Infonet Biovision Home.

Caused by the Clostridium chauvoei bacteria. Symptoms include fever, loss of appetite, stiffness and swelling of affected area ... The clostridia content of this is higher than green legume) are almost pure fibre. Young green plants have less fibre and more ... Caused by clostridium botulinim bacteria;. These bacteria form spores which occur in the soil and can lie dormant for many ... Whilst the clostridium bacteria is present in the intestine of all goats, given favourable conditions (i.e., high levels of ...
more infohttp://www.infonet-biovision.org/AnimalHealth/Goats-new-animal-welfare-information
  • Ingestion of C. chauvoei spores is probably the most common form of exposure and infected ruminants do not directly transmit the disease to other animals. (biomedcentral.com)
  • The nanA gene was found to be conserved and sialidase activity was found in C. chauvoei strains isolated over a period of 50 years from various geographical locations. (biomedcentral.com)
  • For the first time, there is evidence to show that the environmental distribution of Clostridium chauvoei is increased after flooding. (wikipedia.org)
  • 317 aas) is the main cytotoxic and haemolytic substance secreted by C. chauvoei . (tcdb.org)
  • In this study we have analyzed the sialidase activity of the NanA protein of C. chauvoei and cloned the sialidase gene nanA . (biomedcentral.com)