A species of gram-positive, cellulolytic bacteria in the family Clostridiaceae. It produces CELLULOSOMES which are involved in plant CELL WALL degradation.
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Polysaccharides consisting of xylose units.
An exocellulase with specificity for the hydrolysis of 1,4-beta-D-glucosidic linkages in CELLULOSE and cellotetraose. It catalyzes the hydrolysis of terminal non-reducing ends of beta-D-glucosides with release of CELLOBIOSE.
A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.
A common inhabitant of the colon flora in human infants and sometimes in adults. It produces a toxin that causes pseudomembranous enterocolitis (ENTEROCOLITIS, PSEUDOMEMBRANOUS) in patients receiving antibiotic therapy.
Systems of enzymes which function sequentially by catalyzing consecutive reactions linked by common metabolic intermediates. They may involve simply a transfer of water molecules or hydrogen atoms and may be associated with large supramolecular structures such as MITOCHONDRIA or RIBOSOMES.
Infections with bacteria of the genus CLOSTRIDIUM.
Proteins found in any species of bacterium.
A species of anaerobic, gram-positive, rod-shaped bacteria in the family Clostridiaceae that produces proteins with characteristic neurotoxicity. It is the etiologic agent of BOTULISM in humans, wild fowl, HORSES; and CATTLE. Seven subtypes (sometimes called antigenic types, or strains) exist, each producing a different botulinum toxin (BOTULINUM TOXINS). The organism and its spores are widely distributed in nature.
Transport proteins that carry specific substances in the blood or across cell membranes.
An enzyme that catalyzes the hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides. The enzyme plays a role in the lysosomal degradation of the N-glycosylprotein glycans. Defects in the lysosomal form of the enzyme in humans result in a buildup of mannoside intermediate metabolites and the disease BETA-MANNOSIDOSIS.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Enzymes which catalyze the endohydrolysis of 1,4-beta-D-xylosidic linkages in XYLANS.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
A group of enzymes that catalyze the hydrolysis of alpha- or beta-xylosidic linkages. EC 3.2.1.8 catalyzes the endo-hydrolysis of 1,4-beta-D-xylosidic linkages; EC 3.2.1.32 catalyzes the endo-hydrolysis of 1,3-beta-D-xylosidic linkages; EC 3.2.1.37 catalyzes the exo-hydrolysis of 1,4-beta-D-linkages from the non-reducing termini of xylans; and EC 3.2.1.72 catalyzes the exo-hydrolysis of 1,3-beta-D-linkages from the non-reducing termini of xylans. Other xylosidases have been identified that catalyze the hydrolysis of alpha-xylosidic bonds.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A xylosidase that catalyses the random hydrolysis of 1,3-beta-D-xylosidic linkages in 1,3-beta-D-xylans.
An acute inflammation of the INTESTINAL MUCOSA that is characterized by the presence of pseudomembranes or plaques in the SMALL INTESTINE (pseudomembranous enteritis) and the LARGE INTESTINE (pseudomembranous colitis). It is commonly associated with antibiotic therapy and CLOSTRIDIUM DIFFICILE colonization.
A species of gram-positive bacteria in the family Clostridiaceae, used for the industrial production of SOLVENTS.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A publication issued at stated, more or less regular, intervals.
An order of CHLOROPHYTA commonly found in freshwater habitats. Characteristics include the presence of a cellulose wall and two to four equal, smooth, apical flagella.
A four carbon linear hydrocarbon that has a hydroxy group at position 1.
Isomeric forms and derivatives of butanol (C4H9OH).

The interaction of carbohydrate-binding modules with insoluble non-crystalline cellulose is enthalpically driven. (1/15)

Natural cellulose exists as a composite of cellulose forms, which can be broadly characterized as crystalline or non-crystalline. The recognition of both of these forms of cellulose by the CBMs (carbohydrate-binding modules) of microbial glycoside hydrolases is important for the efficient natural and biotechnological conversion of cellulosic biomass. The category of CBM that binds insoluble non-crystalline cellulose does so with an affinity approx. 10-20-fold greater than their affinity for cello-oligosaccharides and/or soluble polysaccharides. This phenomenon has been assumed to originate from the effects of changes in configurational entropy upon binding. The loss of configurational entropy is thought to be less profound upon binding to conformationally restrained insoluble non-crystalline cellulose, resulting in larger free energies of binding. However, using isothermal titration calorimetry, it is shown that this is not the case for the high-affinity interactions of CcCBM17 (the family 17 CBM from EngF of Clostridium cellulovorans) and BspCBM28 (the family 28 CBM from Cel5A of Bacillus species 1139) with regenerated cellulose, an insoluble preparation of primarily non-crystalline cellulose. The enhanced free energy of binding of non-crystalline cellulose relative to cello-oligosaccharides is by virtue of improved enthalpy, not entropy.  (+info)

Isolation and expression of the xynB gene and its product, XynB, a consistent component of the Clostridium cellulovorans cellulosome. (2/15)

The nucleotide sequence of the Clostridium cellulovorans xynB gene, which encodes the XynB xylanase, consists of 1,821 bp and encodes a protein of 607 amino acids with a molecular weight of 65,976. XynB contains a typical N-terminal signal peptide of 29 amino acid residues, followed by a 147-amino-acid sequence that is homologous to the family 4-9 (subfamily 9 in family 4) carbohydrate-binding domain. Downstream of this domain is a family 10 catalytic domain of glycosyl hydrolase. The C terminus separated from the catalytic domain by a short linker sequence contains a dockerin domain responsible for cellulosome assembly. The XynB sequence from mass spectrometry and N-terminal amino acid sequence analyses agreed with that deduced from the nucleotide sequence. XynB was highly active toward xylan, but not active toward carboxymethyl cellulose. The enzyme was optimally active at 40 degrees C and pH 5.0. Northern hybridizations revealed that xynB is transcribed as a monocistronic 1.9-kb mRNA. RNA ligase-mediated rapid amplification of 5' cDNA ends by PCR (RLM-5'RACE PCR) analysis of C. cellulovorans RNA identified a single transcriptional start site of xynB located 47 bp upstream from the first nucleotide of the translation initiation codon. Alignment of the xynB promoter region provided evidence for highly conserved sequences that exhibited strong similarity to the sigmaA consensus promoter sequences of gram-positive bacteria. Expression of xynB mRNA increased from early to middle exponential phase and decreased during the early stationary phase when the cells were grown on cellobiose. No alternative promoter was observed by RLM-5'RACE PCR and reverse transcriptase PCR analyses during expression. The analysis of the products from xylan hydrolysis by thin-layer chromatography indicated its endoxylanase activity. The results suggest that XynB is a consistent and major cellulosomal enzyme during growth on cellulose or xylan.  (+info)

Effect of carbon source on the cellulosomal subpopulations of Clostridium cellulovorans. (3/15)

Clostridium cellulovorans produces a cellulase enzyme complex called the cellulosome. When cells were grown on different carbon substrates such as Avicel, pectin, xylan, or a mixture of all three, the subunit composition of the cellulosomal subpopulations and their enzymic activities varied significantly. Fractionation of the cellulosomes (7-11 fractions) indicated that the cellulosome population was heterogeneous, although the composition of the scaffolding protein CbpA, endoglucanase EngE and cellobiohydrolase ExgS was relatively constant. One of the cellulosomal fractions with the greatest endoglucanase activity also showed the highest or second highest cellulase activity under all growth conditions tested. The cellulosomal fractions produced from cells grown on a mixture of carbon substrates showed the greatest cellulase activity and contained CbpA, EngE/EngK, ExgS/EngH and EngL. High xylanase activity in cellulose, pectin and mixed carbon-grown cells was detected with a specific cellulosomal fraction which had relatively larger amounts of XynB, XynA and unknown proteins (35-45 kDa). These results in toto indicate that the assembly of cellulosomes occurs in a non-random fashion.  (+info)

Degradation of corn fiber by Clostridium cellulovorans cellulases and hemicellulases and contribution of scaffolding protein CbpA. (4/15)

Clostridium cellulovorans, an anaerobic bacterium, degrades native substrates efficiently by producing an extracellular enzyme complex called the cellulosome. All cellulosomal enzyme subunits contain dockerin domains that can bind to hydrophobic domains termed cohesins which are repeated nine times in CbpA, the nonenzymatic scaffolding protein of C. cellulovorans cellulosomes. In this study, the synergistic interactions of cellulases (endoglucanase E, EngE; endoglucanase L, EngL) and hemicellulases (arabinofuranosidase A, ArfA; xylanase A, XynA) were determined on the degradation of corn fiber, a natural substrate containing mainly xylan, arabinan, and cellulose. The degradation by XynA and ArfA of cellulose/arabinoxylan was greater than that of corn fiber and resulted in 2.6-fold and 1.4-fold increases in synergy, respectively. Synergistic effects were observed in increments in both simultaneous and sequential reactions with ArfA and XynA. These synergistic enzymes appear to represent potential rate-limiting enzymes for efficient hemicellulose degradation. When mini-cellulosomes were constructed from the cellulosomal enzymes (XynA and EngL) and mini-CbpA with cohesins 1 and 2 (mini-CbpA1&2) and mini-CbpA with cohesins 5 and 6 (mini-CbpA5&6), higher activity was observed than that for the corresponding enzymes alone. Based on the degradation of different types of celluloses and hemicelluloses, the interaction between cellulosomal enzymes (XynA and EngL) and mini-CbpA displayed a diversity that suggests that dockerin-cohesin interaction from C. cellulovorans may be more selective than random.  (+info)

Site-directed mutagenesis and expression of the soluble form of the family IIIa cellulose binding domain from the cellulosomal scaffolding protein of Clostridium cellulovorans. (5/15)

The planar and anchoring residues of the family IIIa cellulose binding domain (CBD) from the cellulosomal scaffolding protein of Clostridium cellulovorans were investigated by site-directed mutagenesis and cellulose binding studies. By fusion with maltose binding protein, the family IIIa recombinant wild-type and mutant CBDs from C. cellulovorans were expressed as soluble forms. Cellulose binding tests of the mutant CBDs indicated that the planar strip residues played a major role in cellulose binding and that the anchoring residues played only a minor role.  (+info)

Synthesis of Clostridium cellulovorans minicellulosomes by intercellular complementation. (6/15)

The ability of two strains of bacteria to cooperate in the synthesis of an enzyme complex (a minicellulosome) was examined. Three strains of Bacillus subtilis were constructed to express Clostridium cellulovorans genes engB, xynB, and minicbpA. MiniCbpA, EngB, and XynB were synthesized and secreted into the medium by B. subtilis. When the strains with the minicbpA and engB genes or with xynB were cocultured, minicellulosomes were synthesized, consisting in one case of miniCbpA and EngB and in the second case of miniCbpA and XynB. Both minicellulosomes showed their respective enzymatic activities. We call this phenomenon "intercellular complementation." Interesting implications concerning bacterial cooperation are suggested from these results.  (+info)

Synergistic interaction of Clostridium cellulovorans cellulosomal cellulases and HbpA. (7/15)

Clostridium cellulovorans, an anaerobic bacterium, produces a small nonenzymatic protein called HbpA, which has a surface layer homology domain and a type I cohesin domain similar to those found in the cellulosomal scaffolding protein CbpA. In this study, we demonstrated that HbpA could bind to cell wall fragments from C. cellulovorans and insoluble polysaccharides and form a complex with cellulosomal cellulases endoglucanase B (EngB) and endoglucanase L (EngL). Synergistic degradative action of the cellulosomal cellulase and HbpA complexes was demonstrated on acid-swollen cellulose, Avicel, and corn fiber. We propose that HbpA functions to bind dockerin-containing cellulosomal enzymes to the cell surface and complements the activity of cellulosomes.  (+info)

Effect of multiple copies of cohesins on cellulase and hemicellulase activities of Clostridium cellulovorans mini-cellulosomes. (8/15)

Cellulosomes in Clostridium cellulovorans are assembled by the interaction between the repeated cohesin domains of a scaffolding protein (CbpA) and the dockerin domain of enzyme components. In this study, we determined the synergistic effects on cellulosic and hemicellulosic substrates by three different recombinant mini-cellulosomes containing either endoglucanase EngB or endoxylanase XynA bound to mini-CbpA with one cohesin domain (mini-CbpA1), two cohesins (mini-CbpA12), or four cohesins (mini-CbpA1234). The assembly of EngB or XynA with mini-CbpA increased the activity against carboxymethyl cellulose, acid-swollen cellulose, Avicel, xylan, and corn fiber 1.1-1.8-fold compared with that for the corresponding enzyme alone. A most distinct improvement was shown with corn fiber, a natural substrate containing xylan, arabinan, and cellulose. However, there was little difference in activity between the three different mini-cellulosomes when the cellulosomal enzyme concentration was held constant regardless of the copy number of cohesins in the cellulosome. A synergistic effect was observed when the enzyme concentration was increased to be proportional to the number of cohesins in the mini-cellulosome. The highest degree of synergy was observed with mini-CbpA1234 (1.8-fold) and then mini-CbpA12 (1.3-fold), and the lowest synergy was observed with mini-CbpA1 (1.2-fold) when Avicel was used as the substrate. As the copy number of cohesin was increased, there was more synergy. These results indicate that the clustering effect (physical enzyme proximity) of the enzyme within the mini-cellulosome is one of the important factors for efficient degradation of plant cell walls.  (+info)

Clostridium cellulovorans (ATCC 35296) is anaerobic, spore forming and stain gram negative non-motile rods originally isolated from a batch methanogenic fermentation of hybrid poplar wood. C. cellulovorans is a mesophilic bacterium with optimum growth temperature of 37°C, though it can grow in a temperature range of 20 to 40°C. Optimum pH is 7.0, and the pH range of growth is 6.4 to 7.8. This organism produces extracellular enzyme complex known as cellulosome, which can degrade plant cell walls. As most abundantly available potential source of fermentable sugars in the world are the higher plants cell walls, utilization of such a vast resource for energy production would reduce the dependency on non-renewable fossil fuel. Hence, C. cellulovorans have potential industrial application for energy production. ...
Respirable crystalline silica (silicon dioxide; SiO2, quartz) particles are known to induce chronic inflammation and lung disease upon long-term inhalation, whereas non-crystalline (amorphous) SiO2 particles in the submicrometre range are regarded as less harmful. Several reports have demonstrated that crystalline, but also non-crystalline silica particles induce IL-1β release from macrophages via the NALP3-inflammasome complex (caspase-1, ASC and NALP3) in the presence of lipopolysaccharide (LPS) from bacteria. Our aim was to study the potential of different non-crystalline SiO2 particles from the nano- to submicro-sized range to activate IL-1β responses in LPS-primed RAW264.7 macrophages and primary rat lung macrophages. The role of the NALP3-inflammasome and up-stream mechanisms was further explored in RAW264.7 cells. In the present study, we have shown that 6 h exposure to non-crystalline SiO2 particles in nano- (SiNPs, 5-20 nm, 50 nm) and submicro-sizes induced strong IL-1β responses in LPS
ROSSETO, Renato; SANTOS, Ádamo C. M. A. dos and GALEMBECK, Fernando. Hydrous non-crystalline phosphates: structure, function and a new white pigment. J. Braz. Chem. Soc. [online]. 2006, vol.17, n.8, pp.1465-1472. ISSN 0103-5053. http://dx.doi.org/10.1590/S0103-50532006000800002.. Hydrated non-crystalline inorganic solids are often neglected due to the limited comprehension of their complex physico-chemical and structural properties. However, these non-crystalline materials exhibit a rich and varied chemistry, interesting for scientific and technological reasons. This work reviews general aspects of formation of hydrated non-crystalline solids, with special emphasis on aluminum (poly)phosphate materials. Precursors and concentration variations, temperature, ageing and reaction pH are trivial synthetic variables, but they promote the formation of a myriad of compounds adequate for many functions. Amorphous aluminum phosphates are widely employed in different industrial applications, providing ...
In the present study, metabolic engineering and adaptive laboratory evolution were integrated to improve the n-butanol production of C. cellulovorans DSM 743B from AECC, a low-cost and renewable agricultural waste. This represents an application of a previous study of genetic tools of C. cellulovorans DSM 743B in CBP-enabling microbial chassis development (6).. To enable C. cellulovorans DSM 743B to produce n-butanol, the CoA-dependent ABE pathway, including adhE1 and ctfAB-adc genes from C. acetobutylicum ATCC 824, were introduced. The metabolically engineered strains showed increased butanol output compared to results in the study of Yang et al., who overexpressed only adhE2 in C. cellulovorans and eventually achieved 1.42 g/liter n-butanol production from cellulose (28). It is interesting that ZQW03 (743B/pXY1-Pthl-adhE1) exhibited higher n-butanol output, butyraldehyde dehydrogenase activity, and butanol dehydrogenase activity than ZQW02 (743B/pXY1-Pthl-adhE2) (Fig. 2B). The higher butanol ...
iframe src=https://biblio.ugent.be/publication?q=parent+exact+%22JOURNAL+OF+NON-CRYSTALLINE+SOLIDS%22&embed=1&hide_pagination=1&hide_info=1&hide_options=1&hide_cluster=1 ...
Pyrene-box cages easily crystallize from aqueous solutions and readily encapsulate compounds of biological interest. These host-guest systems can be obtained under ambient conditions from 1,3,5,8-pyrenetetrasulfonate (PTS), guanidinium derivatives (G+) and biogenic guests bearing cationic groups. Out of the many examples of synthetic molecular capsules, Pyrene box cages are completely responding to the requirements of green chemistry: non-toxic, water soluble, cheap, commercially available compounds. The Pyrene-Box cages have been used for the in situ encapsulation of unstable or non-crystalline biogenic compounds, allowing the complete molecular structure determination of species that do not crystallize by themselves. The encapsulated guests have a reduced motional degrees of freedom which is obtained via their anchoring to the Pyrene box cages that allows at the same time to reduce a significant amount of disorder. In this highlight we discuss the recent developments of the encapsulation ...
An improvement in production of an optical recording medium of phase change type is provided. In the present method, the step of repeated recording and erasing which had to be carried out after initialization and before shipment in the conventional process is eliminated. The optical recording medium produced by the present process retain good properties after repeated overwriting. In the process of the present invention, the prior art initialization step is replaced by modification treatment and the subsequent crystallization treatment. The modification treatment is a step wherein non-crystalline recording layer formed by sputtering is melted and cooled to change the crystal-lographic state into a non-crystalline state which is different from the non-crystalline state immediately after the formation of the recording layer. The crystallization treatment is a step conducted after the modification treatment wherein the recording layer in non-crystalline state is heated for crystallization.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
Inactive protein inclusion bodies occur commonly in Escherichia coli (E. coli) cells expressing heterologous proteins. Previously several independent groups have found that active protein aggregates or pseudo inclusion bodies can be induced by a fusion partner such as a cellulose binding domain from Clostridium cellulovorans (CBDclos) when expressed in E. coli. More recently we further showed that a short amphipathic helical octadecapeptide 18A (EWLKAFYEKVLEKLKELF) and a short beta structure peptide ELK16 (LELELKLKLELELKLK) have a similar property. In this work, we explored a third type of peptides, surfactant-like peptides, for performing such a
TY - JOUR. T1 - Structure of Zr/Ba/Na fluoride glass using molecular dynamics. AU - Gruenhut, Steven. AU - Amini, M. AU - Macfarlane, Douglas R. AU - Meakin, Pavla. PY - 1997. Y1 - 1997. M3 - Article. SP - 398. EP - 403. JO - Journal of Non-Crystalline Solids. JF - Journal of Non-Crystalline Solids. SN - 0022-3093. ER - ...
TY - JOUR. T1 - Photoluminescence and crystallization in europium-doped fluorobromozircontate glass-ceramics. AU - Edgar, A. AU - Schweizer, S. AU - Assmann, S. AU - Spaeth, J M. AU - Newman, P J. AU - Macfarlane, Douglas R. PY - 2001. Y1 - 2001. U2 - 10.1016/S0022-3093(01)00408-2. DO - 10.1016/S0022-3093(01)00408-2. M3 - Article. VL - 284. SP - 237. EP - 242. JO - Journal of Non-Crystalline Solids. JF - Journal of Non-Crystalline Solids. SN - 0022-3093. IS - 1-3. ER - ...
2,3-dimethyl-8-(2,6-dimethylbenzylamino)-N-hydroxyethyl-irnidazo[l,2-a]pyridine-6-carboxamide mesylate salt form A and form B obtainable according to the present invention are substantially free from other crystalline and non-crystalline forms of 2,3- . dimethyl-8-(2,6-dimethylbenzylamino)-N-hydroxyethyl-imidazo[l,2-a]pyridine-6-carboxamide mesylate salt The term substantially free from other crystalline and non-crystalline forms of 2,3-dimethyl-8-(2,6-dimethylbenzylamino)-N-hydroxyethyl-imidazo[l,2-a]pyridine-6-carboxamide mesylate salt shall be understood to mean that the desired crystal form of 2,3-dimethyl-8-(2,6-dimethylbenzylamino)-N-hydroxyethyl-imidazo[l,2-a]pyridine-6-carboxamide mesylate salt contains less than 10 %, preferably less than 5 %, more preferably less than 3 %, and even more preferably less than 1 % of any other forms of 2,3-dimemyl-8-(2,6-dimemylbenzylarnino)-N-hydroxyemyl-. imidazo[l,2-a]pyridine-6-carboxamide mesylate salt ...
Around the globe, fiber and calcium intakes are below the levels recommended by experts contributing to potential long-term public health implications. New research, published this month in the British Journal of Nutrition, shows soluble corn fiber (SCF) may not simply boost fiber intake when added to foods, but can also increase the amount of beneficial bacteria present in the gut, while enhancing calcium absorption in adolescents.
Complete information for CPA6 gene (Protein Coding), Carboxypeptidase A6, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Esters of .alpha.-fluoroacrylic acid are accessible by hydrolysis of an .alpha.-hydroxymethyl-.alpha.-fluoromalonic acid ester, decarboxylation and dehydration of the hydrolysis product and subsequent esterification of the resulting .alpha.-fluoroacrylic acid with an alcohol. The esters are polymerizable and are suitable for use as a starting material for preparing fluoropolymers which in turn are usable as materials for manufacturing transparent articles. The polymers are high-molecular and non-crystalline and have softening temperatures of above 100.degree. C.
Shungite is a black, lustrous, non-crystalline mineraloid consisting of more than 98 % of carbon. It was first found and described in Karelia, Russia.
A sorbent for use in removing sulfur contaminants from hydrocarbon feedstocks is provided, wherein the sorbent contains zinc aluminate in an amount of at least 40 wt % (calculated as ZnAl2O4); free alumina in an amount of from about 5 wt % to about 25 wt % (calculated as Al2O3); and iron oxide in an amount of from about 10 wt % to about 30 wt % (calculated as Fe2O3); wherein each of the free alumina and iron oxide are present in non-crystalline form as determined by X-ray diffraction analysis, and a method for producing the sorbent and method for using the sorbent to reduce sulfur contaminants in hydrocarbon feedstocks.
Collaborative studies that combine non-crystalline x-ray diffraction with muscle mechanics provide important insights into the mechanism of the classic Frank-Starling relationship. Editor in Chief William C. Stanley and leading expert Richard L. Moss (University of Wisconsin) interview co-authors Pieter de Tombe (Loyola University Chicago) and Thomas Irving (Illinois Institute of Technology) about their innovative research collaboration and their quest to manipulate the contractile state of myofilaments, with implication for the failing heart.. Gerrie P Farman, David Gore, Edward J Allen, Kelly Q Schoenfelt, Thomas C. Irving, and Pieter P. de Tombe. Myosin head orientation: a structural determinant for the Frank-Starling relationship. Am J Physiol Heart Circ Physiol, published ahead of print April 1, 2011; doi:10.1152/ajpheart.01221.2010. ...
Aysawy, M. A. and Szász, András and Kertész, L. and Dankházi, Zoltán (1990) On the metastable states of amorphous fe84-xWxB16 (0,x,5) alloys. JOURNAL OF NON-CRYSTALLINE SOLIDS, 122. pp. 146-150. ISSN 0022-3093 Bajor, Gábor and Czinner, Antal and Dávid, Károly and Földvári, Márta and Gardó, Sándor and Késmárky, József and Leővey, András and Ludwig, Endre and Ribiczey, Pál and Vályi, Péter and Záborszky, Zoltán (1990) A cefotaxim (Claforan) multicentrikus vizsgálata. Gyógyszereink, 40 (9). pp. 170-175. ISSN 0434-9784 Balázs, Mihály and Monok, István and Varga, András (1990) Baranyai Decsi Jánosnak a török elleni háborúra buzdító beszéde 1598-ból. LYMBUS - MAGYARSÁGTUDOMÁNYI FORRÁSKÖZLEMÉNYEK, 2 (11). pp. 37-100. ISSN 0865-0632 Benkő, János (1990) A körutazási feladat megoldása dinamikus programozással. JÁRMŰVEK, MEZŐGAZDASÁGI GÉPEK, 37 (11). pp. 411-413. ISSN 0021-5511 Buchmann, K. and Székely, Csaba and Bjerregaard, J. (1990) Treatment of ...
Get all the key statistics for ROSIER (ENGB.BR), including valuation measures, fiscal year financial statistics, trading records, share statistics and more.
A preparing method of a semiconductor, particularly a preparing method of a polycrystal semiconductor film which has a good electrical property is disclosed. In order to obtain a non-crystalline silicon film containing a lot of combination of hydrogen and silicon, a forming process of a non-crystalline silicon film by a low temperature gas phase chemical reaction, a process of a heat annealing to produce a lot of dangling bonds of silicon, so as to draw out hydrogen from said non-crystalline silicon film, and a process of applying a laser irradiation to said non-crystal silicon film having a lot of dangling bond of silicon are conducted.
VitroClean Green crushed glass filter media from DRAMMwater is manufactured from recycled, processed glass and is a direct replacement for silica sand and other materials in media filters. VitroClean offers you the safest, most reliable and cost effective way to upgrade your new or existing sand filter system. Designed to work in existing sand filter, VitroClean Glass Media will filter finer than sand with 20 percent less media required.. Unlike sand, VitroClean media has a non-crystalline shape offering less density and more open space between grains to trap fine particulate. Because it is crushed glass, it does not degrade like sand, allowing for a greatly reduced need for replacement due to degradation of the media. Even though it is made from crushed glass, VitroClean media is safe to handle, with no jagged edges.. DRAMMwater is a newly formed division of the Dramm Corporation. Focusing on water treatment for horticulture, DRAMMwater uses an engineering based approach and the science behind ...
The skin acts as a physical barrier at the interface with the external environment. This barrier is designed to protect the organism against insults, including desiccant, mechanical, chemical, and microbial damage. It is primarily composed of three layers: the epidermis, dermis, and hypodermis. The epidermis, which contains the stratum corneum (SC), works as a barrier, preventing water loss from the body and protecting it from the environment. The dermis contains fibroblasts as the predominant cell type within a matrix of structural proteins (collagen or elastin), proteoglycans, nervous fibres and sebaceous glands. The hypodermis acts as a thermal and mechanical insulator. Skin contains non-crystalline material, such as collagen, SC lipids and fat, with characteristic scattering patterns that are altered under specific conditions. These changes can be useful for understanding the structure and state of the tissue.. ...
Cryo-electron microscopy is used to discover, or resolve, protein structure. This procedure is a neat tool for imaging proteins that are reluctant to crystallize. It is done by freezing a batch of proteins in liquid ethane and then imaging the non-crystalline ice. Specifically, Walls collects about 1500 micrograph images of the frozen, tumbling proteins in 48 hours. Cryo-EM uniquely enables looking at biological macromolecules in a near-native state, since they are frozen in the shapes that they take on in everyday life. With these images in tow, Walls can then use highly parallelized algorithms to solve for the proteins atomic resolution structures - ultimately a list of amino acid coordinates in three dimensions. The algorithms align, unblur, and average the 2D images of the proteins, and the more images there are of proteins at slightly different tilts and conformations, the more precise the 2D class averages will show the various orientations. The more precise these 2D classes are, the ...
Glass is created by cooling a material to its solid state without allowing it to crystallize, but instead while maintaining a liquid-like flow of atoms. In contrast, the atoms in metals have a neatly arranged crystalline structure. Metallic-glass alloys are non-crystalline solids that contain metallic elements such as zirconium, titanium, copper, or nickel. Such alloys are strong and light, compared to other materials. Unfortunately, they have been difficult to manufacture. ...
A stony-iron meteorite was analyzed using the HyPix-3000. Inside a stony-iron meteorite, there are transparent parts similar to glass and opaque parts similar to metal. The transparent parts were thought to be non-crystalline (amorphous). However, when measurement of the transparent part was actually performed, only one diffraction line was observed. In general, when measurement is done with a 0D or a 1D detector, the range in which diffraction X-rays can be detected from the sample is limited to a certain region. (This region is indicated in yellow in Fig. 1.) In this experiment, the diffraction lines, indicated with red arrows, are within the scanning range, and therefore can be detected. On the other hand, it is possible to acquire information on multiple lattice planes, as shown in Fig. 2, by using the HyPix-3000, which has a wide effective detection area, and by performing measurement while oscillation the sample. When the 2D diffraction image obtained in Fig. 2 was converted to a 2θ-I ...
For centuries, lens-based microscopy, such as light, phase-contrast, fluorescence, confocal and electron microscopy, has played an important role in the evolution of modern science and technology. In 1999, a novel form of microscopy, which is known as coherent diffractive imaging (CDI) or lensless imaging, was developed and transformed our traditional view of microscopy, in which the diffraction pattern of a non-crystalline object or a nanocrystal was first measured and then directly phased to obtain an image. The well-known phase problem was solved by combining the oversampling method with iterative algorithms. In the first part of the talk, I will present the principle of CDI and illustrate some applications using synchrotron radiation, X-ray free electron lasers and high harmonic generation.. In the second part of the talk, I will present a general tomographic method for determining the 3D local structure of materials at atomic resolution. By combining scanning transmission electron ...
A stony-iron meteorite was analyzed using the HyPix-3000. Inside a stony-iron meteorite, there are transparent parts similar to glass and opaque parts similar to metal. The transparent parts were thought to be non-crystalline (amorphous). However, when measurement of the transparent part was actually performed, only one diffraction line was observed. In general, when measurement is done with a 0D or a 1D detector, the range in which diffraction X-rays can be detected from the sample is limited to a certain region. (This region is indicated in yellow in Fig. 1.) In this experiment, the diffraction lines, indicated with red arrows, are within the scanning range, and therefore can be detected. On the other hand, it is possible to acquire information on multiple lattice planes, as shown in Fig. 2, by using the HyPix-3000, which has a wide effective detection area, and by performing measurement while oscillation the sample. When the 2D diffraction image obtained in Fig. 2 was converted to a 2θ-I ...
Irradiated polypropylene articles of non-crystalline mesomorphous polypropylene and a method for preparing the articles are provided. The articles are irradiated with a sterilizing dosage of ionizing radiation. The irradiated articles such as films and blown microfiber webs are substantially undegraded after storage periods of as long as six months.
And this is the second reason I like this paper, and actually the reason I read it in the first place... remember this Nature paper on crystallizing non-crystalline compounds that everyone was stoked on? And then how everyone was less than stoked on? Well, the Buchwald lab stuck with it, and even showed me this Nature Protocols paper by Fujita (that I missed) which details a better protocol for their crystalline sponge method. And, it worked. Cool! I suppose the full value of this method will be realized in time, but this a great (first, to the best of my knowledge) use of Fujitas method outside their own lab. Thus, Buchwald confidently revised the structure of compound 1 to the benzyl alcohol shown (which they refer to as a thioperoxide, this is probably IUPAC, but I dont like it... peroxide to me is O-O... thioperoxide sounds like S-S, but thats disulfide. Now that I think about it, I kind of like dioxide to talk about O-O. I digress. Sorry.). ...
A graduate-level treatment of the science and engineering of Ceramic Materials. Concepts to be studied include the structure of both crystalline and non-crystalline material, and defects (including point defects, dislocations and interfaces) in these materials. A broad range of special (for ceramics) methods for the preparation, processing and characterization of these materials will run throughout the course. An important component of the course is consideration of how the crystal structure determines or influences mechanical, electronic, magnetic, and thermal properties. Special topics may include functional ceramics, 2D ceramics, and connections between ceramics, economics and global affairs ...
Bose-Einstein condensate, IBM research. IBM scientist Thilo Stoferle making adjustments to the dozens of lenses which control the strength of the laser used to create a Bose-Einstein Condensate (BEC). Pioneering work at IBM Research demonstrated this complex quantum mechanical phenomenon using non-crystalline solid materials. The discovery, made using a luminescent polymer, has potential applications in developing novel optoelectronic devices including energy-efficient lasers and optical switches. Photographed in November 2013, at IBM Research-Zurich, Switzerland. - Stock Image C022/8408
In materials science, superplasticity is a state in which solid crystalline material is deformed well beyond its usual breaking point, usually over about 200% during tensile deformation. Such a state is usually achieved at high homologous temperature. Examples of superplastic materials are some fine-grained metals and ceramics. Other non-crystalline materials (amorphous) such as silica glass (molten glass) and polymers also deform similarly, but are not called superplastic, because they are not crystalline; rather, their deformation is often described as Newtonian fluid. Superplastically deformed material gets thinner in a very uniform manner, rather than forming a neck (a local narrowing) that leads to fracture. Also, the formation of microvoids, which is another cause of early fracture, is inhibited.[citation needed] In metals and ceramics, requirements for it being superplastic include a fine grain size (less than approximately 20 micrometres) and a fine dispersion of thermally stable ...
PLA fiber - Poly Lactic Acid Fiber is a new generation of green, environmental-friendly & reproducible fiber with great potential and is 100% biodegradation materi...
荒井 康恵 , 鈴木 彰 , 北本 豊 , 白坂 憲章 , 吉川 賢太郎 , 阪本 禮一郎 , 寺下 隆夫 日本きのこ学会誌 : mushroom science and biotechnology 12(4), 171-177, 2004-12-25 参考文献12件 ...
The authors conducted the first study to test the hypothesized association between biogenic silica fiber (BSF) exposure and malignant mesothelioma. BSF are respirable, non-crystalline silica fibers originating from the mechanical breakdown of plants (e.g., sugarcane and rice). We measured exposures to BSF among workers in the Hawaii sugarcane industry. Harvest tractor drivers have the greatest ave
A stony-iron meteorite was analyzed using the HyPix-3000. Inside a stony-iron meteorite, there are transparent parts similar to glass and opaque parts similar to metal. The transparent parts were thought to be non-crystalline (amorphous). However, when measurement of the transparent part was actually performed, only one diffraction line was observed. In general, when measurement is done with a 0D or a 1D detector, the range in which diffraction X-rays can be detected from the sample is limited to a certain region. (This region is indicated in yellow in Fig. 1.) In this experiment, the diffraction lines, indicated with red arrows, are within the scanning range, and therefore can be detected. On the other hand, it is possible to acquire information on multiple lattice planes, as shown in Fig. 2, by using the HyPix-3000, which has a wide effective detection area, and by performing measurement while oscillation the sample. When the 2D diffraction image obtained in Fig. 2 was converted to a 2θ-I ...
Just as the U.S. EPA announced it was seeking comment on a new rule to establish a pathway for corn fiber to advanced biofuels, two University of Illinois researchers published an analysis of the long waits companies face when petitioning the agency. ...
Lytic polysaccharide monooxygenases (LPMOs) opened a new horizon for biomass deconstruction. They use a redox mechanism not yet fully understood and the range of substrates initially envisaged to be the crystalline polysaccharides is steadily expanding to non-crystalline ones. The enzyme KpLPMO10A from the actinomycete Kitasatospora papulosa was cloned and overexpressed in Escherichia coli cells in the functional form with native N-terminal. The enzyme can release oxidized species from chitin (C1-type oxidation) and cellulose (C1/C4-type oxidation) similarly to other AA10 members from clade II (subclade A). Interestingly, KpLPMO10A also cleaves isolated xylan (not complexed with cellulose, C4-type oxidation), a rare activity among LPMOs not described yet for the AA10 family. The synergistic effect of KpLPMO10A with Celluclast® and an endo-β-1,4-xylanase also supports this finding. The crystallographic elucidation of KpLPMO10A at 1.6 Å resolution along with extensive structural analyses did not
Bioglass 45S5, commonly referred to by its commercial name Bioglass, is a glass specifically composed of 45 wt% SiO2, 24.5 wt% CaO, 24.5 wt% Na2O, and 6.0 wt% P2O5. Glasses are non-crystalline amorphous solids that are commonly composed of silica-based materials with other minor additives. Compared to soda-lime glass (commonly used, as in windows or bottles), Bioglass 45S5 contains less silica and higher amounts of calcium and phosphorus. The 45S5 name signifies glass with 45 weight % of SiO2 and 5:1 molar ratio of calcium to phosphorus. This high ratio of calcium to phosphorus promotes formation of apatite crystals; calcium and silica ions can act as crystallization nuclei. Lower Ca:P ratios do not bond to bone. Bioglass 45S5s specific composition is optimal in biomedical applications because of its similar composition to that of hydroxyapatite, the mineral component of bone. This similarity provides Bioglass ability to be integrated with living bone. This composition of bioactive glass is ...
Dentin is the main component of ivory. Dentin is a calcified tissue of the body, make up largely of mineralized connective tissue and collagen. It is one of the four major components of most teeth and tusks, the others being enamel, cementum, and pulp. Usually, it is covered by enamel on the crown and cementum on the root and surrounds the entire pulp. Teeth and tusks have the same origins. Teeth are specialized structures adapted for food chewing. Tusks are extremely large modified teeth projecting beyond the lips. Teeth and tusks have the same physical structures: pulp cavity, dentine, cementum and enamel. The innermost area is the pulp cavity. The pulp cavity is an empty space within the tooth that conforms to the shape of the pulp. The porous, yellow-hued dentin is made up of about 70 percent inorganic materials (mainly hydroxylapatite and some non-crystalline amorphous calcium phosphate), 20 percent organic materials (90 percent of which is collagen type type and the remaining ten percent ...
39: A method of treating cystic fibrosis, sinusitis, otitis media, ventilator associated pneumonia, chronic bronchitis, chronic obstructive pulmonary disorder, primary ciliary dyskinesia, asthma, bronchiectasis, post-operative atelectasis, Kartageners syndrome, dry mouth, mouth ulcer, gum disease, mycositis, gastro-esophageal reflux disease, peptic ulcer, heartburn, esophagitis, Sjogrens syndrome, inflammatory bowel disease, gastrointestinal problems caused by radiation or chemotherapy for cancer, or a disease associated with expression or activity of a P2Y receptor in a subject, comprising administering to a subject in need thereof a therapeutically effective amount of a compound of formula IIA: ##STR00098## or enantiomers, diastereomers, enantiomerically enriched mixtures, racemic mixtures, crystalline forms, non-crystalline forms, amorphous forms, or pharmaceutically acceptable salts thereof, wherein: A, D, and E are independently N, C(R5), or CH; F is N or C(R4); R1 is an H, oxo, lower ...
SSRL Structural Molecular Biology Group hosts a 3-day comprehensive workshop on the use of non-crystalline small-angle x-ray scattering and diffraction techniques in structural biology research ...
4 Institute for Physics and Competence Center Calorimetry of Interdisciplinary Faculty, University of Rostock, Rostock, Germany. Silk is a naturally occurring biopolymer which has been used in textiles for over 5000 years. Silk stands as an exemplar of the class of fibrous proteins. The properties of silk protein are related to its semicrystalline nature, imparted by the secondary structures, such as the non-crystalline helices and random coils, and the crystalline beta pleated sheets. Using techniques of condensed matter polymer physics, we investigate the structure and thermal behavior of silk fibroin [1-3]. We have prepared fibroin by extracting it from the native cocoons and use this as a starting material for our investigations into the structure and properties of silk. In this presentation, I will describe our studies to quantify the amounts of the different secondary structures using X-ray diffraction, infrared spectroscopy, and high precision, high accuracy heat capacity measurements ...
Non-crystalline solid state and glass. Formation of glass; quenching, condensation. Thermodynamic and kinetic conditions of glass formation. Structure, short-, medium-, and long-rang orders. Glass versus liquid. Glass transition, entropy, heat capacity; kinetics of glass transition. Topology of glass. Short-range order, chemical bond, nearest neighbours, coordination number. Medium-range order, radial distribution function, rings, angle distribution. Long-range order. Glass network and dimension. Chalcogenide glass. One- and two-dimensional solids. Degrees of freedom for chalcogenide and oxide glasses. Rule 8-n and ideal glass. Topological defects and changes in valence. Organic glass. Random Coil and Random Walk models. Scaling exponents, fractal dimension. Metal glass structure. Random Close Packing model, empirically obtained RCP structure. Amorphous silicon and vitreous silica - Continuous Random Network model. Mathematical and chemical bonds - covalent graph, experimental and model RDF. ...
Release your worries by enjoying cups of Bon Temps tea! This blend is wrapped in biodegradable, corn fiber bags to help reduce waste. Just steep for 4 to 6 minutes and you can delight in chamomile and lavender sips to drain your anxieties away and sleep better.20 tea bags per boxBiodegradable | Non-toxic | Organic | Plastic FreeIngredients: Organic and Hand-picked Chamomile and Lavender
Hello! Try adding chloramphenicol to the culture to a final conc. of 1microgram/ml and grow it at 30degC. with best regards, Sulakshana On 13 Dec 2001, shan wrote: , hi. , , I have a problem regarding the solubility of my protein. This protein has a , cellulose binding domain tag. the size of the proein is almost 35kDa. This , protein has huge expression but it is going in the inclusion body in , insoluble form. I tried to solubilize it by 6M urea it became solubalised , but when I dialysed against decrease urea gradient it again became , insoluble. Some one has the idea how to come out of this problem. any , suggestion will be appericiated , , shan , , , ,http://www.biowww.net/forum/read.php?f=1&i=4883&t=4883, , ...
Theresa2021-09-11T08:22:40-06:00September 10th, 2021,. MORE INCENTIVES FOR OWNERSHIP FOR THE 2022 SEASON! The HBPA of Albertas Directors are excited to announce the following program to our eligible participants starting in 2022. The HBPA may amend the program from time ...
In this work, we pro … A detailed overview of the industry with respect to the market size in terms of the volume and remuneration aspects, alongside the current Amorphous Silicon Thin-Film Solar Cell market scenario has been provided in the report. The efficiency is … In its simplest form, the cell structure has a single layers. That is an efficient and production-friendly cell with screen-printed electrodes and thin wires. Crystalline cells are formed out of blocks of solid silicon. Presentation The recent demonstration of high efficiency (exceeding 24%) HIT solar cells developed by Sanyo, Japan 1 1. amorphous silicon is the non-crystalline form of silicon and it can be deposited in thin films at low temperature on to variety of substrates for manufacturing amorphous silicon thin film solar panels. In the hydrogenated amorphous silicon [a-Si:H]-thin film solar cell, large amounts of traps reduce the carriers lifetime that limit the photovoltaic performance, especially the power conversion ...
Every year, over 700 million plastic floss containers end up in our landfills. Our dental floss comes in a simple paper box, is made from corn fibre and is lightly coated with candelilla wax. Because of the nature of eco-friendly fibre, our natural floss tears more readily than plastic nylon products, so it is ideal
Tamaru Y, Doi RH (March 2001). "Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome". ...
January 2011). "Structural and functional analysis of three β-glucosidases from bacterium Clostridium cellulovorans, fungus ...
Clostridium acetobutylicum Clostridium cellulolyticum Clostridium cellulovorans Clostridium clariflavum Clostridium josui ... The scaffoldin of some cellulosomes, an example being that of Clostridium thermocellum, contains a carbohydrate-binding module ... Dockerin Organelle Bayer, EA; Kenig, R; Lamed, R (1983). "Adherence of Clostridium thermocellum to cellulose". J. Bacteriol. ... Clostridium papyrosolvens Clostridium thermocellum (treated as model organism in cellulose utilization and also anaerobic ...
... cellulolyticum Clostridium cellulosi Clostridium cellulovorans Clostridium chartatabidum Clostridium chauvoei ... Clostridium aceticum Clostridium acetireducens Clostridium acetobutylicum Clostridium acidisoli Clostridium aciditolerans ... Clostridium acidurici Clostridium aerotolerans Clostridium aestuarii Clostridium akagii Clostridium aldenense Clostridium ... difficile Clostridium diolis Clostridium disporicum Clostridium drakei Clostridium durum Clostridium estertheticum Clostridium ...
Clostridium cellulolyticum MeSH B03.300.390.400.200.205 - Clostridium cellulovorans MeSH B03.300.390.400.200.215 - Clostridium ... Clostridium cellulolyticum MeSH B03.510.415.400.200.205 - Clostridium cellulovorans MeSH B03.510.415.400.200.215 - Clostridium ... Clostridium symbiosum MeSH B03.300.390.400.200.722 - Clostridium tertium MeSH B03.300.390.400.200.725 - Clostridium tetani MeSH ... Clostridium symbiosum MeSH B03.510.415.400.200.722 - Clostridium tertium MeSH B03.510.415.400.200.725 - Clostridium tetani MeSH ...
"Clostridium cellulovorans" at the Encyclopedia of Life LPSN Type strain of Clostridium cellulovorans at BacDive - the Bacterial ... Clostridium cellulovorans is an anaerobic, mesophilic, spore-forming cellulolytic bacterium. Its cells are gram-negative and ... Koukiekolo R, Cho HY, Kosugi A, Inui M, Yukawa H, Doi RH (July 2005). "Degradation of corn fiber by Clostridium cellulovorans ... Doi RH, Tamaru Y (2001). "The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity ...
"Clostridium cellulovorans" at the Encyclopedia of Life LPSN Type strain of Clostridium cellulovorans at BacDive - the Bacterial ... Clostridium cellulovorans is an anaerobic, mesophilic, spore-forming cellulolytic bacterium. Its cells are gram-negative and ... Koukiekolo R, Cho HY, Kosugi A, Inui M, Yukawa H, Doi RH (July 2005). "Degradation of corn fiber by Clostridium cellulovorans ... Doi RH, Tamaru Y (2001). "The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity ...
Number of predicted genes was the largest in C. cellulovorans as compared to other cellulosomal clostridia. C. cellulovorans ... Clostridium cellulovorans is non pathogenic to human beings. References. Bayer, E. A., Shimon, L. J. W., Shoham, Y. and Lamed, ... Clostridium cellulovorans. From MicrobeWiki, the student-edited microbiology resource. Revision as of 02:44, 20 April 2011 by ... Blair, B. G. and Anderson, K. L. "Regulation of cellulose-inducible structures of Clostridium cellulovorans. Can J Microbiol. ...
Other name: Clostridium cellulovorans strain 743B Description and Significance. Clostridium cellulovorans (ATCC 35296) is ... Number of predicted genes was the largest in C. cellulovorans as compared to other cellulosomal clostridia. C. cellulovorans ... Clostridium cellulovorans is non pathogenic to human beings. References. Bayer, E. A., Shimon, L. J. W., Shoham, Y. and Lamed, ... Clostridium cellulovorans. From MicrobeWiki, the student-edited microbiology resource. Revision as of 01:09, 20 April 2011 by ...
... the properties of the Clostridium cellulovorans cellulosome. The C cellulovorans cellulosome is an extracellular enzyme complex ... The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity.. Doi RH1, Tamaru Y. ...
... such as Clostridium cellulolyticum and Clostridium cellulovorans (12, 17, 18), have also been suggested to have the potential ... fermentation from alkali extracted deshelled corn cobs by co-culture of Clostridium beijerinckii and Clostridium cellulovorans ... Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. Proc Natl Acad Sci U S A 89:3483-3487. doi: ... Structure, dynamics, and specificity of endoglucanase D from Clostridium cellulovorans. J Mol Biol 425:4267-4285. doi:10.1016/j ...
Transcriptomic Responses of the Interactions between Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009 in a ... Transcriptomic Responses of the Interactions between Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009 in a ... Transcriptomic Responses of the Interactions between Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009 in a ... Comparison of the mesophilic cellulosome-producing Clostridium cellulovorans genome with other cellulosome-related clostridial ...
Identification Of Mannanase From Clostridium Cellulovorans And Application Of Cellulosome Complex For Hydrolysis Of ...
63446 in Clostridium cellulovorans 743B. Domain architectures illustrate each occurrence of 51445,63446. ... Domain combinations for 51445,63446 superfamilies in Clostridium cellulovorans 743B. The selected domain combination is the ... 9 sequences contain the 51445,63446 domain architecture in Clostridium cellulovorans 743B. ...
Transcription of Clostridium cellulovorans cellulosomal cellulase and hemicellulase genes. Sung Ok Han, Hideaki Yukawa, ... Transcription of Clostridium cellulovorans cellulosomal cellulase and hemicellulase genes. / Han, Sung Ok; Yukawa, Hideaki; ... Transcription of Clostridium cellulovorans cellulosomal cellulase and hemicellulase genes. Journal of Bacteriology. 2003 Apr; ... Han, S. O., Yukawa, H., Inui, M., & Doi, R. H. (2003). Transcription of Clostridium cellulovorans cellulosomal cellulase and ...
... from Clostridium cellulovorans 743B. Plus protein sequence and external database links. ... Domain assignment for gi,302875546,ref,YP_003844179.1, from Clostridium cellulovorans 743B. Domain architecture ... glucan endo-1,3-beta-D-glucosidase [Clostridium cellulovorans 743B]. Sequence. ...
Structures of exoglucanase from Clostridium cellulovorans: cello-tetraose binding and cleavage. L.-C. Tsai, I. Amiraslanov, H.- ...
Unlike other cellulosome-producing Clostridium species, C. cellulovorans can metabolize all major plant cell wall ... cellulovorans. This data will be valuable in understanding strategies employed by C. cellulovorans for degrading major plant ... cellulovorans to reveal strategies underlying plant cell wall polysaccharide degradation. We cultured C. cellulovorans with ... However, we identified pectate lyase as a unique enzyme in C. cellulovorans cultivated on xylan, which was not previously ...
Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of ... We performed a focused proteome analysis of cellulosomal proteins predicted by a genome analysis of Clostridium cellulovorans [ ... We sequenced the entire genome of Clostridium cellulovorans (Tamaru et al. [2010a]) and identified all genes, including those ... Doi RH, Tamaru Y: The Clostridium cellulovorans cellulosome: an enzyme complex with plant cell wall degrading activity. Chem ...
Unique contribution of the cell wall-binding endoglucanase G to the cellulolytic complex in Clostridium cellulovorans. Applied ... Unique contribution of the cell wall-binding endoglucanase G to the cellulolytic complex in Clostridium cellulovorans. In: ... Unique contribution of the cell wall-binding endoglucanase G to the cellulolytic complex in Clostridium cellulovorans. / Jeon, ... The cellulosomes produced by Clostridium cellulovorans are organized by the specific interactions between the cohesins in the ...
Structural and functional analysis of three β-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei ... Structural and functional analysis of three β-glucosidases from bacterium Clostridium cellulovorans, fungus Trichoderma reesei ...
Revision history of Proizvodnja n-butanola in etanola iz celuloze v bakteriji Clostridium cellulovorans s prekomernim ... cellulovorans_s_prekomernim_izra%C5%BEanjem_aldehid_in_alkohol_dehidrogenaz" ... Retrieved from "http://wiki.fkkt.uni-lj.si/index.php/Proizvodnja_n-butanola_in_etanola_iz_celuloze_v_bakteriji_Clostridium_ ...
h2,Proizvodnja alifatskih alkoholov v Clostridium cellulovorans,/h2,. ,h2,Proizvodnja alifatskih alkoholov v Clostridium ... Proizvodnja alifatskih alkoholov v Clostridium cellulovorans. Cilj avtorjev, na delu katerih temelji ta seminar, je bila ... Kot odskočno desko so si izbrali bakterijo Clostridium cellulovorans. Kot namiguje že njeno ime, ima celulolitično aktivnost, ... Proizvodnja n-butanola in etanola iz celuloze v bakteriji Clostridium cellulovorans s prekomernim izražanjem aldehid in alkohol ...
The Clostridium cellulovorans cellulosome.Crit. Rev. Microbiol.2019948793. OpenUrlCrossRefPubMed ... The cellulosome paradigm, developed largely from the study of Clostridium spp., is the most firmly established example of a ... Adherence of Clostridium thermocellum to cellulose.J. Bacteriol.1561983818827. ... andClostridium perfringens (16). The Pil-like proteins in B. subtilis and S. gordonii are involved in competence factor- ...
... from Clostridium cellulolyticum; PelA (AF105330) and ExgS (U34793) from Clostridium cellulovorans; and Clostridium josui CelD ( ... Primary sequence analysis of Clostridium cellulovorans cellulase binding protein A. Proc. Natl. Acad. Sci. USA 89:3483-3487. ... Rumsp-Xyn1), and a selection of enzymes from C. thermocellum and mesophilic clostridia (C. cellulolyticum, C. cellulovorans, ... Cohesin-dockerin interactions of cellulosomal subunits of Clostridium cellulovorans. J. Bacteriol. 183:5431-5435. ...
Structure, Dynamics, and Specificity of Endoglucanase D from Clostridium cellulovorans  Bianchetti, Christopher M.; Brumm, ...
Structure, Dynamics, and Specificity of Endoglucanase D from Clostridium cellulovorans  Bianchetti, Christopher M.; Brumm, ...
EXPRESSION AND SECRETION OF CLOSTRIDIUM CELLULOVORANS XYNA IN SACCHAROMYCES CEREVISIAE - Batt, S.B., Wong, D., Wagschal, K.C., ... Expression and secretion of clostridium cellulovorans xyna in saccharomyces cerevisiae. Division of Biological Chemistry, ...
Tamaru Y, Doi RH (March 2001). "Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome". ...
Synergistic effects on crystalline cellulose degradation between cellulosomal cellulases from Clostridium cellulovorans. J. ... from a newly isolated thermophilic aerobic bacterium Caldibacillus cellulovorans gen. nov., sp. nov. World J. Microbiol. ...
... alcoholic fermentation using recombinant Saccharomyces cerevisiae engineered for the production of Clostridium cellulovorans ...
Species: Clostridium cellulovorans [TaxId:1493]. Gene: BGLA. Database cross-references and differences (RAF-indexed): *Uniprot ... Species: Clostridium cellulovorans [TaxId:1493]. Gene: BGLA. Database cross-references and differences (RAF-indexed): *Uniprot ... Species: Clostridium cellulovorans [TaxId:1493]. Gene: BGLA. Database cross-references and differences (RAF-indexed): *Uniprot ... Species: Clostridium cellulovorans [TaxId:1493]. Gene: BGLA. Database cross-references and differences (RAF-indexed): *Uniprot ...
Clostridium cellulovorans. , OCM 3 T OCM 3 is type strain of:. Clostridium cellulovorans. ...
Cloning of Clostridium cellulovorans endo-1,4-beta-glucanase genes. Biochem. Biophys. Res. Commun. 169:667-672. ... Cellulose binding domain from Clostridium cellulovorans as a paper modification reagent. Nordic Pulp Paper Res. J. 18:421-428. ... Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A. J. Bacteriol. ... Two cellulose binding modules from Clostridium cellulovorans were fused together to form a cellulose cross-linking protein (CCP ...
Clostridium cellulovorans, Clostridium josui, Clostridium phytofermentans, and C. thermocellum (8-11). Cellulases from family ... 1998) Properties of exgS, a gene for a major subunit of the Clostridium cellulovorans cellulosome. Gene 211:39-47. ... Deletion of the Cel48S cellulase from Clostridium thermocellum. Daniel G. Olson, Shital A. Tripathi, Richard J. Giannone, ... Deletion of the Cel48S cellulase from Clostridium thermocellum. Daniel G. Olson, Shital A. Tripathi, Richard J. Giannone, ...
Surprisingly, genetic organization of this large cluster is very similar to that of Clostridium cellulolyticum, the model of ... A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. ... Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A.. *Oded Shoseyov, Masahiro Takagi, Michael A ... Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A.. *Michael A ...
  • Recent analysis of the endA gene from Ruminococcus flavefaciens revealed a distant relationship between regions of an 80-amino-acid sequence in EndA and the duplicated 23-amino-acid dockerin sequences found in Clostridium thermocellum cellulosomal enzymes ( 17 ). (asm.org)
  • In Clostridium thermocellum , which has provided the paradigm for cellulosome organization, the complex is integrated via the binding of dockerin-containing enzyme subunits to nine cohesin domains present in a noncatalytic scaffolding protein (CipA). (asm.org)
  • Clostridium thermocellum is a thermophilic anaerobic bacterium that rapidly solubilizes cellulose with the aid of a multienzyme cellulosome complex. (pnas.org)
  • Clostridium thermocellum has received consideration in the context of the latter strategy. (pnas.org)
  • Family 48 glycoside hydrolase (GH48) enzymes are highly expressed in truly cellulolytic bacteria ( 7 ) including Clostridium cellulolyticum , Clostridium cellulovorans , Clostridium josui , Clostridium phytofermentans , and C. thermocellum ( 8 - 11 ). (pnas.org)
  • LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313. (semanticscholar.org)
  • Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homology. (semanticscholar.org)
  • Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS. (semanticscholar.org)
  • The emergence of Clostridium thermocellum as a high utility candidate for consolidated bioprocessing applications. (biofueljournal.com)
  • Effect of key factors on hydrogen production from cellulose in a co-culture of Clostridium thermocellum and Clostridium thermopalmarium. (biofueljournal.com)
  • The interactions between the cohesin of the Clostridium thermocellum CipA scaffolding protein and the dockerin of cellulosomal catalytic components are categorized as type I, and the interactions between the dockerin of CipA and its counterpart are categorized as type II. (asmscience.org)
  • Indeed, interspecies specificity was demonstrated using cohesins and dockerins from C. thermocellum and Clostridium cellulolyticum , respectively. (asmscience.org)
  • An alternative mechanism for the bioconversion of cellulose was found in quite a few anaerobic microorganisms such as Clostridium thermocellum, which employs a large multiprotein complex (cellulosome) to hydrolyze cellulose [ 3 ]. (biomedcentral.com)
  • Adherence of Clostridium thermocellum to cellulose. (nchu.edu.tw)
  • Xylanases Y (XynY) and Z (xynZ) from Clostridium thermocellum. (expasy.org)
  • Endo-β-1,3-1,4 glucanase (licB) from Clostridium thermocellum. (expasy.org)
  • Cellulose integrating protein (cipA) from Clostridium thermocellum. (expasy.org)
  • Cellulose degradation by Clostridium thermocellum: from manure to molecular biology. (expasy.org)
  • Calcium-binding affinity and calcium-enhanced activity of Clostridium thermocellum endoglucanase D. (expasy.org)
  • 10] E.S. Edward A. Bayer, Raphael Lamed, Organization and Distribution of the Cellulosome in Clostridium thermocellum, J. Bacteriol. (nchu.edu.tw)
  • 11] E.S. Raphael Lamed, Rina Kenig, Edward A. Bayer, The cellulosome: a discrete cell surface organelle of Clostridium thermocellum which exhibits separate antigenic, cellulose-binding and various cellulolytic activities, Biotechnol. (nchu.edu.tw)
  • When grown in cellulose, C. cellulovorans forms ultrastructural protuberances, which may be aggregation of smaller cellulosome complexes, also known as polycellulosomes. (kenyon.edu)
  • This review summarizes some of the general properties of cellulosomes, and more specifically, the properties of the Clostridium cellulovorans cellulosome. (nih.gov)
  • The C cellulovorans cellulosome is an extracellular enzyme complex with a molecular weight of about 1 x 10(6), and is comprised of at least ten subunits. (nih.gov)
  • Clostridium cellulovorans is a mesophilic, cellulosome-producing bacterium containing 57 genomic cellulosomal enzyme-encoding genes. (biomedcentral.com)
  • Unlike other cellulosome-producing Clostridium species, C. cellulovorans can metabolize all major plant cell wall polysaccharides (cellulose, hemicelluloses, and pectins). (biomedcentral.com)
  • C. cellulovorans can degrade all types of major plant cell wall polysaccharides (cellulose, hemicelluloses, and pectins) using the cellulosome [ 10 ]. (biomedcentral.com)
  • Proteome analysis of the C. cellulovorans cellulosome after culture in various carbon sources demonstrated the production of carbon source-adapted cellulosome components. (springeropen.com)
  • The cellulosome, which is produced by many cellulolytic gram-positive anaerobic bacteria such as Clostridium , efficiently degrades plant cell wall polysaccharides. (springeropen.com)
  • In this study, a novel analysis using the long monolithic column was applied to a proteome analysis focused on the cellulosome of C. cellulovorans to characterize the molecular mechanism that underlies efficient degradation of various biomasses. (springeropen.com)
  • The cellulosome paradigm, developed largely from the study of Clostridium spp. (asm.org)
  • Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. (semanticscholar.org)
  • Cloning and DNA sequencing of the genes encoding Clostridium josui scaffolding protein CipA and cellulase CelD and identification of their gene products as major components of the cellulosome. (semanticscholar.org)
  • Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation. (dbcls.jp)
  • Clostridium cellulovorans DSM 743B offers potential as a chassis strain for biomass refining by consolidated bioprocessing (CBP). (asm.org)
  • IMPORTANCE Due to a lack of genetic tools, Clostridium cellulovorans DSM 743B has not been comprehensively explored as a putative strain platform for n -butanol production by consolidated bioprocessing (CBP). (asm.org)
  • In this study, next-generation sequencing was used to query the global transcriptomic responses of an artificial coculture of Clostridium cellulovorans 743B and Rhodopseudomonas palustris CGA009. (asm.org)
  • C. cellulovorans 743B (ATCC 35296) was grown anaerobically as described (Robert et al. (springeropen.com)
  • The aim of this work was to use Clostridium cellulovorans 743B to saccharify lignocellulose and produce butyric acid, instead of adding cellulase and butyric acid to the medium, so that the soluble sugars and butyric acid generated can be subsequently utilized by Clostridium beijerinckii NCIMB 8052 to produce butanol in one pot reaction. (storysteel.gq)
  • The issue of secretion in heterologous expression of Clostridium cellulolyticum cellulase-encoding genes in Clostridium acetobutylicum ATCC 824. (semanticscholar.org)
  • Endoglucanases A (celCCA), C (celCCC), D (celCCD), F (celCCF) and G (celCCG) from Clostridium cellulolyticum. (expasy.org)
  • 13] M. Desvaux, Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia, FEMS Microbiol Rev, 29 (2005) 741-764. (nchu.edu.tw)
  • Isolation and Characterization of an Anaerobic, Cellulolytic Bacterium, Clostridium cellulovorans sp. (wikipedia.org)
  • In this study, a high yield expression (70-250 mg/l) in Escherichia coli of the three functional β-glucosidase genes was obtained from the bacterium Clostridium cellulovorans (CcBglA), the fungus Trichoderma reesei (TrBgl2), and the termite Neotermes koshunensis (NkBgl) with the crystal structures of CcBglA, TrBgl2 and NkBgl, determined at 1.9Å, 1.63Å and 1.34Å resolution, respectively. (rcsb.org)
  • Clostridium cellulovorans is an anaerobic, mesophilic, spore-forming cellulolytic bacterium. (wikipedia.org)
  • C. cellulovorans is a mesophilic bacterium with optimum growth temperature of 37°C, though it can grow in a temperature range of 20 to 40°C. Optimum pH is 7.0, and the pH range of growth is 6.4 to 7.8. (kenyon.edu)
  • Clostridium termitidis CT1112 is an anaerobic, Gram-positive, mesophilic, spore-forming, cellulolytic bacterium, originally isolated from the gut of a wood feeding termite Nasusitermes lujae . (biomedcentral.com)
  • Characterization of the extracellular cellulase from a mesophilic clostridium (strain C7). (nchu.edu.tw)
  • Endoglucanase B (engB) from Clostridium cellulovorans. (expasy.org)
  • Data on the electrophoretic study of the protein structure of organs and tissues Determination of subunit composition of Clostridium cellulovorans cellulosomes that degrade plant cell walls. (pianolarge.gq)
  • Transcription of the cellulosomal cellulase/hemicellulase genes of Clostridium cellulovorans has been investigated by Northern blot, reverse transcriptase PCR (RT-PCR), primer extension, and S1 nuclease analysis. (elsevier.com)
  • Doi, Roy H. / Transcription of Clostridium cellulovorans cellulosomal cellulase and hemicellulase genes . (elsevier.com)
  • Although high-molecular-mass cellulase complexes have also been identified in a variety of other anaerobic bacteria, including Bacteroides cellulosolvens , Fibrobacter succinogenes , and Ruminococcus albus ( 7 , 18 ), it is still unclear to what extent the Clostridium spp. (asm.org)
  • Purification and characterization of a cellulase (CMCase) from a newly isolated thermophilic aerobic bacterium Caldibacillus cellulovorans gen. nov., sp. (springer.com)
  • Cellulase, clostridia, and ethanol. (semanticscholar.org)
  • Clostridium cellulovorans (ATCC 35296) is anaerobic, spore forming and stain gram negative non-motile rods originally isolated from a batch methanogenic fermentation of hybrid poplar wood. (kenyon.edu)
  • This study demonstrates the construction of a coenzyme A (CoA)-dependent acetone-butanol-ethanol (ABE) pathway in C. cellulovorans by introducing adhE1 and ctfA-ctfB-adc genes from Clostridium acetobutylicum ATCC 824, which enabled it to produce n -butanol using the abundant and low-cost agricultural waste of alkali-extracted, deshelled corn cobs (AECC) as the sole carbon source. (asm.org)
  • A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. (semanticscholar.org)
  • 57 cellulosomal genes were reported in C. cellulovorans . (kenyon.edu)
  • Number of predicted genes was the largest in C. cellulovorans as compared to other cellulosomal clostridia. (kenyon.edu)
  • C. cellulovorans contains large number of genes encoding non-cellulosomal enzymes which are more associated with polysaccharides (such as hemicelluloses and pectins) degradation than to cellulose. (kenyon.edu)
  • By analyzing differentially regulated gene expression, we showed that, consistent with the physiological observations of enhanced H 2 production and cellulose degradation, the nitrogen fixation genes in R. palustris and the cellulosomal genes in C. cellulovorans were upregulated in cocultures. (asm.org)
  • A number of genes related to biosynthesis of volatile fatty acids (VFAs) in C. cellulovorans were upregulated, and correspondingly, a gene that mediates organic compound catabolism in R. palustris was also upregulated. (asm.org)
  • In addition, genes responsible for sulfur and thiamine metabolism in C. cellulovorans were downregulated in cocultures, and this could be due to a response to pH changes. (asm.org)
  • For example, studies have demonstrated that cocultures containing dark-fermentative bacteria such as Clostridium species and photosynthetic bacteria such as Rhodopseudomonas species can enhance hydrogen (H 2 ) production and substrate consumption ( 16 - 22 ). (asm.org)
  • The organization of microbial plant cell wall-degrading enzymes into multienzyme complexes or cellulosomes was first demonstrated at the molecular level in cellulolytic Clostridium species ( 2 , 4 , 5 , 12 , 14 , 20 , 23 , 34 , 40 , 41 ). (asm.org)
  • On the basis of these inconclusive criteria, species of the genus Clostridium reflect a large heterogeneous group with pheno- and genotypical diversity. (alertamigratoria.org)
  • The engL gene cluster of Clostridium cellulovorans contains a gene for cellulosomal manA. (semanticscholar.org)
  • CBD of C. cellulovorans cellulose binding protein gene with const. (igem.org)
  • Cellulose binding Domain (CBDclos) of Clostridium cellulovorans cellulose binding protein gene (cbp A) in Standard 25 (Freiburg) under the constitutive Promotor J23100 with the RBS J61101. (igem.org)
  • The prototypical members of your group I lasso peptides incorporate siamycin I ,siamycin II and RP ,all of which possess two disulfide bonds and an NterminalTable Detected thiopeptide gene clusterPhylum Clostridium cellulovorans B Propionibacterium acnes KPA Firmicutes Actinobacteria Locus Tag of modifying enzyme Lan Cloc. (calpaininhibitor.com)
  • In this study, we performed a temporal proteome analysis of C. cellulovorans to reveal strategies underlying plant cell wall polysaccharide degradation. (biomedcentral.com)
  • However, we identified pectate lyase as a unique enzyme in C. cellulovorans cultivated on xylan, which was not previously associated with xylan degradation. (biomedcentral.com)
  • Genome sequencing of C. cellulovorans has been completed. (kenyon.edu)
  • We sequenced the entire genome of Clostridium cellulovorans (Tamaru et al. (springeropen.com)
  • However, we have only general subcellular proteinous information regarding the proteins that actively degrade biomass, although genome analysis has provided many interesting insights into the characteristics of C. cellulovorans . (springeropen.com)
  • Fermentation of single and mixed substrates by the parent and an acid-tolerant, mutant strain of Clostridium thermoaceticum . (c56technologies.com)
  • Molecular detection of the clostridia in an anaerobic biohydrogen fermentation system by hydrogenase mRNA-targeted reverse transcription-PCR. (nchu.edu.tw)
  • S.T. Yang, I.C. Tang, and M.R. Okos, Kinetics of Homoacetic Fermentation of Lactate by Clostridium formicoaceticum , Appl. (osu.edu)
  • S.T. Yang, I.C. Tang, and M.R. Okos, Kinetics and Mathematical Modeling of Homoacetic Fermentation of Lactate by Clostridium formicoaceticum , Biotechnol. (osu.edu)
  • I.C. Tang, M.R. Okos, and S.T. Yang, Effects of pH and Acetic Acid on Homoacetic Fermentation of Lactate by Clostridium formicoaceticum , Biotechnol. (osu.edu)
  • Cassava bagasse hydrolysate containing mainly glucose was obtained after treatments with dilute acid and enzymes (glucoamylases and cellulases) and then supplemented with corn steep liquor for use as substrate in repeated-batch fermentation with engineered Clostridium tyrobutyricum CtΔack-adhE2 in a fibrous-bed bioreactor. (bvsalud.org)
  • Using response surface method, 20 runs of experiments were carried out to investigate the effects of initial pH variation (5-8), temperature (25-40°C), and glucose concentration (4-12 g/l) on biohydrogen production in dark fermentation method by Clostridium acetobutylicum (PTCC 1492). (alertamigratoria.org)
  • Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A. (semanticscholar.org)
  • The cellulosomes produced by Clostridium cellulovorans are organized by the specific interactions between the cohesins in the scaffolding proteins and the dockerins of the catalytic components. (elsevier.com)
  • In addition to cellulosomal proteins, C. cellulovorans also secretes non-cellulosomal proteins to degrade plant cell wall polysaccharides. (biomedcentral.com)
  • This data will be valuable in understanding strategies employed by C. cellulovorans for degrading major plant cell wall polysaccharides. (biomedcentral.com)
  • T. Bao, J. Zhao, J. Li, X. Liu in S. T. Yang: ''n''-butanol and ethanol production from cellulose by Clostridium cellulovorans overexpressing heterologous aldehyde/alcohol dehydrogenases. (uni-lj.si)
  • This method represents a milestone toward n -butanol production by CBP, using a single recombinant clostridium strain. (asm.org)
  • Based on the previous study of genetic tools, strain engineering of C. cellulovorans for the development of a CBP-enabling microbial chassis was demonstrated in this study. (asm.org)
  • The n -butanol production from AECC was increased 138-fold, from less than 0.025 g/liter to 3.47 g/liter, which represents the highest titer of n -butanol produced using a single recombinant clostridium strain by CBP reported to date. (asm.org)
  • We cultured C. cellulovorans with five different carbon sources (glucose, cellulose, xylan, galactomannan, and pectin) and performed proteome analysis on cellular and secreted proteins. (biomedcentral.com)
  • In case of secreted proteins of C. cellulovorans cultured either on cellulose or xylan, galactomannan, and pectin, the clusters with the most increasing trend contained either 25 cellulosomal proteins and five non-cellulosomal proteins or 8-19 cellulosomal proteins and 9-16 non-cellulosomal proteins, respectively. (biomedcentral.com)
  • In this study, we clarified, for the first time, the temporal proteome dynamics of cellular and secreted proteins in C. cellulovorans . (biomedcentral.com)
  • I.C. Tang, S.T. Yang, and M.R. Okos, Acetic Acid Production from Whey Lactose by the Coculture of Streptococcus lactis and Clostridium formicoaceticum , Appl. (osu.edu)
  • S.T. Yang and I.C. Tang, Methanogenesis from Lactate by a Coculture of Clostridium formicoaceticum and Methanosarcina mazei , Appl. (osu.edu)
  • V sorodni bakteriji ''Clostridium acetobutylicum'', ki velja za modelni mikroorganizem za preučevanje fermentacije, pri kateri nastajajo aceton, etanol in butanol (''angl. (uni-lj.si)
  • Binding to LacNAc (β-D-galactosyl-1,4-β-D-N-acetylglucosamine) has been shown for an N-acetylglucosaminidase from Clostridium perfingens (PMID: 16990278). (cazy.org)
  • Then, a novel adaptive laboratory evolution (ALE) approach was adapted to strengthen the n -butanol tolerance of C. cellulovorans to fully utilize its n -butanol output potential. (asm.org)
  • The n -butanol production from AECC of the engineered C. cellulovorans was increased 138-fold, from less than 0.025 g/liter to 3.47 g/liter. (asm.org)
  • Metabolic engineering and evolutionary engineering were integrated to improve the n -butanol production of C. cellulovorans from the low-cost renewable agricultural waste of alkali-extracted, deshelled corn cobs (AECC). (asm.org)
  • J. F. Wang, X. R. Yang, C. C. Chen in S. T. Yang: Engineering clostridia for butanol production from biorenewable resources: from cells to preocess integration. (uni-lj.si)
  • Optimization of biohydrogen production by Clostridium butyricum EB6 from palm oil mill effluent using response surface methodology. (biofueljournal.com)
  • These results indicated that in C. cellulovorans, EngG assemble into both the cellulolytic complex and the cell wall complex to aid in the hydrolysis of cellulose substrates. (elsevier.com)
  • Han SO, Yukawa H, Inui M, Doi RH (2005) Molecular cloning and transcriptional and expression analysis of engO, encoding a new noncellulosomal family 9 enzyme, from Clostridium cellulovorans . (springer.com)
  • From sequence analysis, cel 2 belongs to glycosyl hydrolase family 5 and exhibit high similarity to engD and engO from Clostridium cellulovorans and celB from Ruminococcus albus. (pjbt.org)
  • Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. (semanticscholar.org)
  • The 3rd ORF had 10 regions of homology with cellulose-binding protein A from Clostridium cellulovorans. (nih.gov)
  • Using a cohesin biomarker, we identified a cellulosomal enzyme which belongs to the glycosyl hydrolase family 5 and has a domain of unknown function 291 (DUF291) with functions similar to those of the surface layer homology domain in C. cellulovorans. (elsevier.com)