Clostridium cellulolyticum: A species of gram-positive bacteria in the family Clostridiaceae. It is a cellulolytic, mesophilic species isolated from decayed GRASS.Clostridium: A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.Cellulose: A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.Cellulase: An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.Cellulosomes: Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.Cellobiose: A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.Cellulases: A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.Biomass: Total mass of all the organisms of a given type and/or in a given area. (From Concise Dictionary of Biology, 1990) It includes the yield of vegetative mass produced from any given crop.Clostridium difficile: A common inhabitant of the colon flora in human infants and sometimes in adults. It produces a toxin that causes pseudomembranous enterocolitis (ENTEROCOLITIS, PSEUDOMEMBRANOUS) in patients receiving antibiotic therapy.Fermentation: Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.Clostridium Infections: Infections with bacteria of the genus CLOSTRIDIUM.Clostridium acetobutylicum: A species of gram-positive bacteria in the family Clostridiaceae, used for the industrial production of SOLVENTS.Culture Media: Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.Bacterial Proteins: Proteins found in any species of bacterium.Clostridium botulinum: A species of anaerobic, gram-positive, rod-shaped bacteria in the family Clostridiaceae that produces proteins with characteristic neurotoxicity. It is the etiologic agent of BOTULISM in humans, wild fowl, HORSES; and CATTLE. Seven subtypes (sometimes called antigenic types, or strains) exist, each producing a different botulinum toxin (BOTULINUM TOXINS). The organism and its spores are widely distributed in nature.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Carboxymethylcellulose Sodium: A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.Kinetics: The rate dynamics in chemical or physical systems.Libraries, Digital: Libraries in which a major proportion of the resources are available in machine-readable format, rather than on paper or MICROFORM.Job Satisfaction: Personal satisfaction relative to the work situation.KansasSelf Concept: A person's view of himself.Stress, Psychological: Stress wherein emotional factors predominate.Questionnaires: Predetermined sets of questions used to collect data - clinical data, social status, occupational group, etc. The term is often applied to a self-completed survey instrument.Professional Autonomy: The quality or state of being independent and self-directing, especially in making decisions, enabling professionals to exercise judgment as they see fit during the performance of their jobs.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.Access to Information: Individual's rights to obtain and use information collected or generated by others.Journal Impact Factor: A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.Bibliometrics: The use of statistical methods in the analysis of a body of literature to reveal the historical development of subject fields and patterns of authorship, publication, and use. Formerly called statistical bibliography. (from The ALA Glossary of Library and Information Science, 1983)Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.Peer Review, Research: The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.Algorithms: A procedure consisting of a sequence of algebraic formulas and/or logical steps to calculate or determine a given task.Hydrogenase: An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.Clostridium thermocellum: A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.Hydrogen: The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.Glucose: A primary source of energy for living organisms. It is naturally occurring and is found in fruits and other parts of plants in its free state. It is used therapeutically in fluid and nutrient replacement.Crystallization: The formation of crystalline substances from solutions or melts. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Crystallography, X-Ray: The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)X-Ray Diffraction: The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)Colicins: Bacteriocins elaborated by strains of Escherichia coli and related species. They are proteins or protein-lipopolysaccharide complexes lethal to other strains of the same species.Ribosome Inactivating Proteins: N-Glycosidases that remove adenines from RIBOSOMAL RNA, depurinating the conserved alpha-sarcin loop of 28S RIBOSOMAL RNA. They often consist of a toxic A subunit and a binding lectin B subunit. They may be considered as PROTEIN SYNTHESIS INHIBITORS. They are found in many PLANTS and have cytotoxic and antiviral activity.Ribosome Inactivating Proteins, Type 1: Ribosome inactivating proteins consisting of only the toxic A subunit, which is a polypeptide of around 30 kDa.Ribosome Inactivating Proteins, Type 2: Ribosome inactivating proteins consisting of two polypeptide chains, the toxic A subunit and a lectin B subunit, linked by disulfide bridges. The lectin portion binds to cell surfaces and facilitates transport into the ENDOPLASMIC RETICULUM.Molecular Weight: The sum of the weight of all the atoms in a molecule.Salts: Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed)Chemical Precipitation: The formation of a solid in a solution as a result of a chemical reaction or the aggregation of soluble substances into complexes large enough to fall out of solution.Alcohol Drinking: Behaviors associated with the ingesting of alcoholic beverages, including social drinking.1-Propanol: A colorless liquid made by oxidation of aliphatic hydrocarbons that is used as a solvent and chemical intermediate.NADH Tetrazolium Reductase: Catalyzes the reduction of tetrazolium compounds in the presence of NADH.Beer: An alcoholic beverage usually made from malted cereal grain (as barley), flavored with hops, and brewed by slow fermentation.Biofuels: Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).Portal Vein: A short thick vein formed by union of the superior mesenteric vein and the splenic vein.Yeasts: A general term for single-celled rounded fungi that reproduce by budding. Brewers' and bakers' yeasts are SACCHAROMYCES CEREVISIAE; therapeutic dried yeast is YEAST, DRIED.
Structural insights into the mechanism of formation of cellulosomes probed by small angle X-ray scattering. (1/23)Exploring the mechanism by which the multiprotein complexes of cellulolytic organisms, the cellulosomes, attain their exceptional synergy is a challenge for biologists. We have studied the solution structures of the Clostridium cellulolyticum cellulosomal enzyme Cel48F in the free and complexed states with cohesins from Clostridium thermocellum and Clostridium cellulolyticum by small angle x-ray scattering in order to investigate the conformational events likely to occur upon complexation. The solution structure of the free cellulase indicates that the dockerin module is folded, whereas the linker connecting the catalytic module to the dockerin is extended and flexible. Remarkably, the docking of the different cohesins onto Cel48F leads to a pleating of the linker. The global structure determined here allowed modeling of the atomic structure of the C. cellulolyticum dockerin-cohesin interface, highlighting the local differences between both organisms responsible for the species specificity. (+info)
Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin. (2/23)In recent work, we reported the self-assembly of a comprehensive set of defined "bifunctional" chimeric cellulosomes. Each complex contained the following: (i) a chimeric scaffoldin possessing a cellulose-binding module and two cohesins of divergent specificity and (ii) two cellulases, each bearing a dockerin complementary to one of the divergent cohesins. This approach allowed the controlled integration of desired enzymes into a multiprotein complex of predetermined stoichiometry and topology. The observed enhanced synergy on recalcitrant substrates by the bifunctional designer cellulosomes was ascribed to two major factors: substrate targeting and proximity of the two catalytic components. In the present work, the capacity of the previously described chimeric cellulosomes was amplified by developing a third divergent cohesin-dockerin device. The resultant trifunctional designer cellulosomes were assayed on homogeneous and complex substrates (microcrystalline cellulose and straw, respectively) and found to be considerably more active than the corresponding free enzyme or bifunctional systems. The results indicate that the synergy between two prominent cellulosomal enzymes (from the family-48 and -9 glycoside hydrolases) plays a crucial role during the degradation of cellulose by cellulosomes and that one dominant family-48 processive endoglucanase per complex is sufficient to achieve optimal levels of synergistic activity. Furthermore cooperation within a cellulosome chimera between cellulases and a hemicellulase from different microorganisms was achieved, leading to a trifunctional complex with enhanced activity on a complex substrate. (+info)
Molecular cloning and transcriptional and expression analysis of engO, encoding a new noncellulosomal family 9 enzyme, from Clostridium cellulovorans. (3/23)Clostridium cellulovorans produces a major noncellulosomal family 9 endoglucanase EngO. A genomic DNA fragment (40 kb) containing engO and neighboring genes was cloned. The nucleotide sequence contained reading frames for endoglucanase EngO, a putative response regulator, and a putative sensor histidine kinase protein. The engO gene consists of 2,172 bp and encodes a protein of 724 amino acids with a molecular weight of 79,474. Northern hybridizations revealed that the engO gene is transcribed as a monocistronic 2.6-kb mRNA. 5' RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) PCR analysis indicated that the single transcriptional start site of engO was located 264 bp upstream from the first nucleotide of the translation initiation codon. Alignment of the engO promoter region provided evidence for highly conserved sequences that exhibited strong similarity to the sigma(A) consensus promoter sequences of gram-positive bacteria. EngO contains a typical N-terminal signal peptide of 28 amino acid residues, followed by a 149-amino-acid sequence which is homologous to the family 4-9 carbohydrate-binding domain. Downstream of this domain was an immunoglobulin-like domain of 89 amino acids. The C terminus contains a family 9 catalytic domain of glycosyl hydrolase. Mass spectrometry analysis of EngO was in agreement with that deduced from the nucleotide sequence. Expression of engO mRNA increased from early to middle exponential phase and decreased during the early stationary phase. EngO was highly active toward carboxymethyl cellulose but showed no activity towards xylan. It was optimally active at 40 to 50 degrees C and pH 5 to 6. The analysis of the products from the cellulose hydrolysis through thin-layer chromatography indicated its endoglucanase activity. (+info)
Structural basis of cellulosome efficiency explored by small angle X-ray scattering. (4/23)Cellulose, the main structural component of plant cell walls, is the most abundant carbohydrate polymer in nature. To break down plant cell walls, anaerobic microorganisms have evolved a large extracellular enzyme complex termed cellulosome. This megadalton catalytic machinery organizes an enzymatic assembly, tenaciously bound to a scaffolding protein via specialized intermodular "cohesin-dockerin" interactions that serve to enhance synergistic activity among the different catalytic subunits. Here, we report the solution structure properties of cellulosome-like assemblies analyzed by small angle x-ray scattering and molecular dynamics. The atomic models, generated by our strategy for the free chimeric scaffoldin and for binary and ternary complexes, reveal the existence of various conformations due to intrinsic structural flexibility with no, or only coincidental, inter-cohesin interactions. These results provide primary evidence concerning the mechanisms by which these protein assemblies attain their remarkable synergy. The data suggest that the motional freedom of the scaffoldin allows precise positioning of the complexed enzymes according to the topography of the substrate, whereas short-scale motions permitted by residual flexibility of the enzyme linkers allow "fine-tuning" of individual catalytic domains. (+info)
Transcriptional analysis of the cip-cel gene cluster from Clostridium cellulolyticum. (5/23)Twelve genes encoding key components of Clostridium cellulolyticum cellulosomes are clustered. Among them, the first, second, and fifth genes encode the assembly factor CipC and the two major cellulases Cel48F and Cel9E, respectively. Cellulolytic clones were selected from the noncellulolytic cipC insertional mutant trans-complemented with a cipC expression vector, in which one homologous recombination event between the 3' end of the chromosomal cipC gene and the plasmidic cipC gene has restored the cluster continuity. The absence of the enzymes encoded by the cluster in the cipC mutant was thus only due to a strong polar effect, indicating that all genes were transcriptionally linked. Two large transcripts were detected in cellulose-grown cells by Northern hybridization: a 14-kb messenger which carries the cipC-cel48F-cel8C-cel9G-cel9E coding sequences and, in a smaller amount, a 12-kb messenger which carries the genes located in the 3' part of the cluster. Four smaller transcripts were found in large amounts: a cipC-cel48F bicistronic one and three monocistronic ones, cipC, cel48F, and cel9E. The cipC-cel48F and cel48F messengers were shown to be stable. Analysis by reverse transcription-PCR suggested transcriptional linkage of all of the open reading frames. The production of a primary very large transcript covering the entire cluster was hypothesized. Primer extension analysis has identified two putative transcriptional start sites located 638/637 and 194 nucleotides upstream of the cipC translational start. The processing of the primary transcript would lead to the production of several secondary messengers displaying different stabilities, contributing to fine tuning of expression of individual genes of the operon. (+info)
Enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum explored by two-dimensional analysis: identification of seven genes encoding new dockerin-containing proteins. (6/23)The enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum grown on crystalline cellulose as a sole carbon and energy source was explored by two-dimensional electrophoresis. The cellulolytic system of C. cellulolyticum is composed of at least 30 dockerin-containing proteins (designated cellulosomal proteins) and 30 noncellulosomal components. Most of the known cellulosomal proteins, including CipC, Cel48F, Cel8C, Cel9G, Cel9E, Man5K, Cel9M, and Cel5A, were identified by using two-dimensional Western blot analysis with specific antibodies, whereas Cel5N, Cel9J, and Cel44O were identified by using N-terminal sequencing. Unknown enzymes having carboxymethyl cellulase or xylanase activities were detected by zymogram analysis of two-dimensional gels. Some of these enzymes were identified by N-terminal sequencing as homologs of proteins listed in the NCBI database. Using Trap-Dock PCR and DNA walking, seven genes encoding new dockerin-containing proteins were cloned and sequenced. Some of these genes are clustered. Enzymes encoded by these genes belong to glycoside hydrolase families GH2, GH9, GH10, GH26, GH27, and GH59. Except for members of family GH9, which contains only cellulases, the new modular glycoside hydrolases discovered in this work could be involved in the degradation of different hemicellulosic substrates, such as xylan or galactomannan. (+info)
Incorporation of fungal cellulases in bacterial minicellulosomes yields viable, synergistically acting cellulolytic complexes. (7/23)Artificial designer minicellulosomes comprise a chimeric scaffoldin that displays an optional cellulose-binding module (CBM) and bacterial cohesins from divergent species which bind strongly to enzymes engineered to bear complementary dockerins. Incorporation of cellulosomal cellulases from Clostridium cellulolyticum into minicellulosomes leads to artificial complexes with enhanced activity on crystalline cellulose, due to enzyme proximity and substrate targeting induced by the scaffoldin-borne CBM. In the present study, a bacterial dockerin was appended to the family 6 fungal cellulase Cel6A, produced by Neocallimastix patriciarum, for subsequent incorporation into minicellulosomes in combination with various cellulosomal cellulases from C. cellulolyticum. The binding of the fungal Cel6A with a bacterial family 5 endoglucanase onto chimeric miniscaffoldins had no impact on their activity toward crystalline cellulose. Replacement of the bacterial family 5 enzyme with homologous endoglucanase Cel5D from N. patriciarum bearing a clostridial dockerin gave similar results. In contrast, enzyme pairs comprising the fungal Cel6A and bacterial family 9 endoglucanases were substantially stimulated (up to 2.6-fold) by complexation on chimeric scaffoldins, compared to the free-enzyme system. Incorporation of enzyme pairs including Cel6A and a processive bacterial cellulase generally induced lower stimulation levels. Enhanced activity on crystalline cellulose appeared to result from either proximity or CBM effects alone but never from both simultaneously, unlike minicellulosomes composed exclusively of bacterial cellulases. The present study is the first demonstration that viable designer minicellulosomes can be produced that include (i) free (noncellulosomal) enzymes, (ii) fungal enzymes combined with bacterial enzymes, and (iii) a type (family 6) of cellulase never known to occur in natural cellulosomes. (+info)
Evolution of acetoclastic methanogenesis in Methanosarcina via horizontal gene transfer from cellulolytic Clostridia. (8/23)Phylogenetic analysis confirmed that two genes required for acetoclastic methanogenesis, ackA and pta, were horizontally transferred to the ancestor of Methanosarcina from a derived cellulolytic organism in the class Clostridia. This event likely occurred within the last 475 million years, causing profound changes in planetary methane biogeochemistry. (+info)
... is an anaerobic, motile, gram-positive bacterium. "Clostridium cellulolyticum: Petitdemange et al. ... "Clostridium cellulolyticum". Retrieved 2011-07-07. Type strain of Clostridium cellulolyticum at BacDive - the Bacterial ...
"A rhamnogalacturonan lyase in the Clostridium cellulolyticum cellulosome". J. Bacteriol. 185: 4727-4733. doi:10.1128/jb.185.16. ...
To make the conversion possible, researchers had to develop a strain of Clostridium cellulolyticum, a native cellulose- ... "Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose". Applied and Environmental ... Clostridium While cellulosic biomass like corn stover and switchgrass is abundant and cheap, it is much more difficult to ...
Bacillus polymyxa Chromobacterium violaceum Clostridium beijerinckii Clostridium cellulolyticum Pseudomonas putida While this ... Acetoacetate decarboxylase from Clostridium acetobutylicum catalyzes the decarboxylation of acetoacetate to yield acetone and ... In 1916, biochemist and future first president of Israel Chaim Weizmann was the first to isolate Clostridium acetobutylicum, a ... Acetoacetate decarboxylase has been found and studied in the following bacteria in addition to Clostridium acetobutylicum: ...
Clostridium acetobutylicum Clostridium cellulolyticum Clostridium cellulovorans Clostridium clariflavum Clostridium josui ... The scaffoldin of some cellulosomes, an example being that of Clostridium thermocellum, contains a carbohydrate-binding module ... Clostridium papyrosolvens Clostridium thermocellum (treated as model organism in cellulose utilization and also anaerobic ... "Adherence of Clostridium thermocellum to cellulose". J. Bacteriol. 2: 818-827. Bayer EA, Belaich JP, Shoham Y, and Lamed R. The ...
List of MeSH codes (B03)
Clostridium butyricum MeSH B03.300.390.400.200.200 --- Clostridium cellulolyticum MeSH B03.300.390.400.200.205 --- Clostridium ... Clostridium butyricum MeSH B03.510.415.400.200.200 --- Clostridium cellulolyticum MeSH B03.510.415.400.200.205 --- Clostridium ... Clostridium symbiosum MeSH B03.300.390.400.200.722 --- Clostridium tertium MeSH B03.300.390.400.200.725 --- Clostridium tetani ... Clostridium symbiosum MeSH B03.510.415.400.200.722 --- Clostridium tertium MeSH B03.510.415.400.200.725 --- Clostridium tetani ...
Regulation of cel genes of C. cellulolyticum: identification of GlyR2, a transcriptional regulator regulating cel5D gene...
Transcription and expression regulation of some individual cel genes (cel5A, cel5I, cel5D and cel44O) of Clostridium cellulolyticum were investigated. Unlike the cip-cel operon, these genes are transcribed as monocistronic units of transcription, except cel5D. The location of the transcription initiation sites was determined using RT-PCR and the mRNA 5′-end extremities were detected using primer extension experiments. Similarly to the cip-cel operon, cel5A and cel5I expressions are regulated by a carbon catabolite repression mechanism, whereas cel44O and cel5D expressions do not seem to be submitted to this regulation. The role of the putative transcriptional regulator GlyR2 in the regulation of cel5D expression was investigated. The recombinant protein GlyR2 was produced and was shown to bind in vitro to the cel5D and glyR2 promoter regions, suggesting that besides regulating its own expression, GlyR2 may regulate cel5D expression. To test this hypothesis in vivo, an insertional glyR2 mutant ...
Sequence Similarity - 1G9J: X-TAL STRUCTURE OF THE MUTANT E44Q OF THE CELLULASE CEL48F IN COMPLEX WITH A...
1G9J: Structures of mutants of cellulase Cel48F of Clostridium cellulolyticum in complex with long hemithiocellooligosaccharides give rise to a new view of the substrate pathway during processive action
Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by...
The rise in oil prices and environmental concerns has heightened interest in the microbial production of fuels and chemicals from sugar feedstocks produced from renewable biomass. Branched higher alcohols are both representative promising next-generation biofuels and building blocks for producing a variety of chemicals [1,2]. In particular, isobutanol can be used as a fuel, fuel additive, and a commodity chemical, and thus is an important biorefinery target alcohol. Furthermore, isobutanol has attractive properties, including lower toxicity and higher octane value than its straight-chain counterpart .. Metabolically engineered microbial strains for producing isobutanol have been developed by introducing parts of the Ehrlich pathway into bacterial hosts such as Escherichia coli, Corynebacterium glutamicum, Clostridium cellulolyticum, and Bacillus subtilis [3-8]. In these recombinant strains, an intermediate of valine biosynthesis, 2-ketoisovalerate, is converted into isobutanol through ...
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Even with 10-year old boots, I was still getting heal blisters after a few thousand feet of gain. I tried moleskin, one sock, two socks, knee-highs under socks, special cross lacing, nothing helped. I put ENGO in the heels of my boots with two pairs of socks, and hiked the entire 212-mile John Muir Trail without a single blister. Im now a complete believer that preventing sweaty sock stick to the boot (or foot) is the key ...
Tobacco plants were used to produce a fungal cellulase, TrCel5A, via a transient expression system. The expression could be monitored...
Bioprocessing is a technology used in a variety of industries. This course will provide an overview of various industrial uses of microbial bioprocessing. The course will present bioprocessing in industries ranging from the food and pharmaceutical industries to the chemical and green-tech industries. The course is designed to teach students the basic fundamentals of a bioprocess and what they need to know about the similarities and the unique aspects of bioprocessing in each industry covered.. Prerequisites. None. Learning Objectives. ...
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NILS STOLPE: The New England groundfish debacle (Part IV): Is cutting back harvest really the answer?. While its a fact thats hardly ever acknowledged, the assumption in fisheries management is that if the population of a stock of fish isnt at some arbitrary level, its because of too much fishing. Hence the term "overfished." Hence the mandated knee jerk reaction of the fisheries managers to not enough fish; cut back on fishing. What of other factors? They dont count. Its all about fishing, because fishing is all that the managers can control; its their Maslows Hammer. When it comes to the oceans it seems as if its about all that the industry connected mega-foundations that support the anti-fishing ENGOs with hundreds of millions of dollars a year in "donations" are interested in controlling. Read the article here. ...
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Agilent Technologies, University of Rouen and A*STAR's Bioprocessing Technology Institute Collaborate | Technology Networks
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RCSB PDB - 1EGZ: CELLULASE CEL5 FROM ERWINIA CHRYSANTHEMI, A FAMILY GH 5-2 ENZYME Structure Summary Page
1EGZ: Type II protein secretion in gram-negative pathogenic bacteria: the study of the structure/secretion relationships of the cellulase Cel5 (formerly EGZ) from Erwinia chrysanthemi
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Unresolved security issues could seriously affect a companys data in a regulated environment, so iron-clad anti-hacking protection needs to be in place. Unfortunately, cyber security is not yet a top focus for the bioindustry.
Choose from the University approved GEP U.S. Diversity course list or choose a course identified on the approved GEP course lists as meeting the U.S. Diversity (USD) co-requisite. The following course(s) completed as part of the Major requirements may fulfill this requirement: None ...
Microbial systems are capable of producing substantial volumes of bulk chemicals at levels competitive with chemical synthetic protocols.
Bioprocessing of Renewable Resources to Commodity Bioproducts / Hoboken, NJ, USA : John Wiley & Sons, Inc., 2014, ; ISSN: ; ISBN: 9781118175835 ; doi:10.1002/9781118845394 Hoboken, NJ, USA : John Wiley & Sons, Inc., 437-474 (2014) [10.1002/9781118845394.ch16] BibTeX , EndNote: XML, Text , RIS Contribution to a book ...
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Bioprocessing / Fermentation (Target Discovery) > Bioprocessing Research Services Laboratory Product Directory and Equipment...
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The most significant equipment-related trend in bioprocessing over the past 15 years has been the replacement of reusable glass and steel
Science, 2016, 351, 507-510 An unprecedented mechanism of nucleotide methylation in organisms containing thyX Tatiana V. Mishanina,1 Liping Yu,2Kalani Karunaratne,1 Dibyendu Mondal,1 John M. Corcoran,1Michael A. Choi,1Amnon Kohen1† 1Department of Chemistry, University of Iowa, Iowa City, IA52242, USA.
UK-based MS instrument provider has partnered with the CPI to directly interface its mass spectrometer with a bioreactor for real-time monitoring
Dr. ÖMER BARIŞ YÜCEL; Kocaeli Hastanesi lokasyonunda Üroloji bölümünde çalışmaktadır. Bilgi ve randevu için tıklayın.
Evolution of high cellulolytic activity in symbiotic Streptomyces through selection of expanded gene content and coordinated...
In this study, the evolution of cellulose degradation was a defining event in the history of life. Without efficient decomposition and recycling, dead plant biomass would quickly accumulate and become inaccessible to terrestrial food webs and the global carbon cycle. On land, the primary drivers of plant biomass deconstruction are fungi and bacteria in the soil or associated with herbivorous eukaryotes. While the ecological importance of plant-decomposing microbes is well established, little is known about the distribution or evolution of cellulolytic activity in any bacterial genus. Here we show that in Streptomyces, a genus of Actinobacteria abundant in soil and symbiotic niches, the ability to rapidly degrade cellulose is largely restricted to two clades of host-associated strains and is not a conserved characteristic of the Streptomyces genus or host-associated strains. Our comparative genomics identify that while plant biomass degrading genes (CAZy) are widespread in Streptomyces, key ...
Structural Biochemistry/Cell Organelles/Plant Cell/Cellulose - Wikibooks, open books for an open world
Cellulosomes known as the proficient nanomachine in nature are cell bound multi-enzyme complexes that break down cellulose and hemicelluloses. They are very important in the process of carbon turnover. The cellulosome complexes require highly ordered proteins (the interactions between cohesions and dockerins) to assembly cellulases and hemicellulases into a scaffold structure. The protein interactions between cohesion and dockerin play an important role in cellulosome assembly and the attachment of cellulosome to the surface of cells while remaining flexible to provide a stable catalytic synergy.. One function of cellulosomes is breaking down plants structural polysaccharides. It is hypothesize that the constraints of the cellulosomes system created by bacteria and fungi caused the deconstruction of plant cell wall to become more and more efficient. The splicing of the plant-cell wall involves the addition of enzymes on to a macromolecule complex will increase the effectiveness of the ...
The 2018 Gordon Research Seminar on Cellulosomes, Cellulases and Other Carbohydrate Modifying Enzymes (GRS) will be held in Andover, NH. Apply today to reserve your spot.
ENGOs parent company, Tamarack Habilitation Technologies, has appropriate security measures in place to protect against the loss, misuse or alteration of information that we have collected from you at our site. We use SSL certificates to protect your information.. Unless provided via phone, email or fax, Tamarack Habilitation Technologies does not have access to your credit card number. If such information is provided for a purchase or refund to an authorized Tamarack (ENGOs parent company) employee, the company ensures the information will be used as directed. The information will then be shredded to maintain the safety of all our customers private information. We do not keep customer credit card information on file electronically or in print.. ...
Standal, Rune; Iversen, Tore Geir; Coucheron, Dag-Hugo; Fjærvik, Espen; Blatny, Janet Martha; Valla, Svein. (1994) A new gene required for cellulose production and a gene encoding cellulolytic activity in Acetobacter xylinum are colocalized with the bcs operon. Journal of Bacteriology. vol. 176. ...
DEPT.:Medicinal Chemistry YEAR OF GRAD STUDY: 2007-2012 MENTOR(S): Kevin Rice SOURCE(S) OF SUPPORT: NIH T32, PI Grants, AFPE, College of Pharmacy (UI) EDUCATION: Carleton College BA 2006 Chemistry University of Iowa (UI) PhD 2012 Medicinal Chemistry
Consolidated bioprocessing, or CBP, the conversion of lignocellulose into desired items in a single step without added enzymes, is a subject of enhanced analysis effort recently. Inside review, the economic...
Oxford Global are proud to present the 8th Annual Cell Culture & Bioprocessing Congress, taking place on during 29 - 30 October 2019, Novotel London West, London, UK
Small angle X-ray scattering study of carbon nanotube forests densified into long range patterns by controlled solvent...
Dumee, Ludovic F., Sears, Kallista, Schutz, Jurg A., Finn, Niall, Duke, Mikel, Mudie, Stephen, Kirby, Nigel and Gray, Stephen 2013, Small angle X-ray scattering study of carbon nanotube forests densified into long range patterns by controlled solvent evaporation, Journal of colloid and interface science, vol. 407, pp. 556-560, doi: 10.1016/j.jcis.2013.06.018. ...
The interaction between cellulase enzymes and their substrates is of central importance to several technological and scientific challenges. Here we report that the binding of cellulose binding modules (CBM) from Trichoderma reesei cellulases Cel6A and Cel7A show a major difference in how they interact with substrates originating from wood compared to bacterial cellulose. We found that the CBM from TrCel7A recognizes the two substrates differently and as a consequence shows an unexpected way of binding. We show that the substrate has a large impact on the exchange rate of the studied CBM, and moreover, CBM-TrCel7A seems to have an additional mode of binding on wood derived cellulose but not on cellulose originating from bacterial source. This mode is not seen in double CBM (DCBM) constructs comprising both CBM-TrCel7A and CBM-TrCel6A. The linker length of DCBMs affects the binding properties, and slows down the exchange rates of the proteins and thus, can be used to analyze the differences ...
Bioprocessing / Fermentation (Target Discovery) > Bioreactors Laboratory Product Directory and Equipment Reviews on...
Read reviews and compare manufacturers of Bioprocessing / Fermentation (Target Discovery) > Bioreactors products in the SelectScience products and suppliers directory
Bioethanol Production from Brewers Spent Grains Using a Fungal Consolidated Bioprocessing (CBP) Approach | SpringerLink
Production of bioethanol from brewers spent grains (BSG) using consolidated bioprocessing (CBP) is reported. Each CBP system consists of a primary filamentous fungal species, which secretes the enzyme
General Information: Country: Japan; Isolation: Methanogenic sludge; Temp: Thermophile; Temp: 55C. Clostridium clariflavum is a thermophilic cellulose degrader closely related to C. thermocellum, with equivalent cellulolytic activity. ...
When it comes to scaling-up your big idea, make sure you have a partner thats as comfortable in the production suite as the lab. Choose Corning and build your success on our deep foundation of cell culture expertise.. When it comes to scaling-up your big idea, make sure you have a partner thats as comfortable in the production suite as the lab. Choose Corning and build your success on our deep foundation of cell. ...
Separation unit operations make up a critical portion of biologics downstream processes. Complexity inherently associated with biologics manufacture such as product heterogeneity and host impurities presents process challenges not typically encountered in small-molecule separations. With bioreactor titers increasing steadily over the last several years, production cost and operational pressures are shifting further to downstream. In addition, biological metabolites are still produced in relatively low amounts. The development of cost- and time-effective bioseparation and concentration unit ops is thus key to efficient downstream processes. This session will focus on highlighting the latest advances in bioseparations and downstream processing, aimed at cost reduction, improved efficiency, and shortend development time. Topics to be covered include, but are not limited to: Centrifugation; Crystallization, evaporation, and precipitation; Membrane-based separations; Adsorption and ion exchange; ...
Automation of CAR-T cell adoptive immunotherapy bioprocessing: Technology opportunities to debottleneck manufacturing -...
Mediatech Inc., a Corning Subsidiary, is a manufacturer of sterile solutions ranging from cell culture media, molecular biology reagents, bioprocessing containment systems, and custom manufacturing.
Mediatech Inc., a Corning Subsidiary, is a manufacturer of sterile solutions ranging from cell culture media, molecular biology reagents, bioprocessing containment systems, and custom manufacturing.
As a design and build provider with specialist expertise in upstream, advanced fermenters/bioreactors , downstream processing  and filling/packing operations, Bouygues Energies & Services guarantees the seamless delivery of biopharmaceutical facilities for our customers.. Whether the requirement is for monoclonal, enzymatic, antibiotic, transgenic, gene therapy or therapeutic protein bioprocessing facilities, we have all of the in-house, multi-disciplinary  skills and resources necessary to guarantee product efficacy and bio-safety.. ...
Health, ... Article in Spring edition of Bioprocessing Journal reports InVitria...Fort Collins CO (PRWEB) April 22 2009-- Recent studies using both cl...Additional findings from the study showed that Cellastim improved the...This research incorporated classical media as well as several l...,InVitria,Reveals,Technology,to,Optimize,Hybridoma,Production,Performance,medicine,medical news today,latest medical news,medical newsletters,current medical news,latest medicine news
Theo Melas-Kyriazi is CFO of Levitronix Technologies LLC, a leader in magnetically levitated pumps for microelectronics and bioprocessing applications. He ..
Typical problem areas in footwear are under the heel and forefoot, and at the side of the heel. An oval patch can be applied to overlap the side of the heel counter and the insole as seen is the photo. I often use a rectangle or large oval under the ball of the foot or an oval under the heel - applied directly to the insole. The patches are useful over stitching or seams in footwear that are rubbing the wearer. If necessary, a patch can be cut to shape for where it will be applied.. The patches will reduce shear and friction; provide relief from hot spot and blister pain, and can be used in any type of insole or orthotic and footwear, from sandals to running shoes, and any type of hiking or ski boot.. I like ENGO patches because they work. The patch is thin and does not alter the fit of the shoe. When properly applied to dry footwear, they stick.. Rebecca Rushton, a podiatrist in Australia, strongly recommends ENGO Patches. She discovered the patches after getting blisters herself and now ...
This Industrial Bioprocessing TOE depicts trends across food waste recycling. This issue features overall regional trends in the adoption and development of food waste recycling technologies, drivers and restraints for the adoption of these technologies,
Some of my research emphasis at UK is in bioprocessing, specifically downstream processing of value added proteins and the conversion of biomass to chemicals and fuels. My other area of interest is in biomass conversion. The conversion of agricultural and forestry biomass into value-added chemicals and materials holds great promise for increasing industrial sustainability and increasing markets available for U.S. producers. In one project, we are focusing on the thermochemical conversion of biomass to value added chemicals and materials by catalytic mediated liquefaction (CML), emphasizing the yield of heavy products.. Keywords: Bioprocessing, Biofuels, Fermentation, Algae Cultivation, Downstream Processing. ...
Research in the Bioprocessing and Membrane Separations Lab is focused on the purification of high value biological products and the application of membrane systems for bioseparations. This includes fundamental studies of membrane transport and fouling, as well as the application of these principles to the development of downstream processes for purification of recombinant proteins, gene therapy agents, and vaccines.. ...
Bioproduction Media, Supplements and Feed Manufacturing. As part of BDs global network of manufacturing centers, BDAB is focused on the development and manufacturing of bioproduction media and supplements. Our full suite of manufacturing services are tailored to meet the needs of your production process - from rapid small-scale medium production to full-scale GMP medium manufacturing.. We offer flexibility and capacity without sacrificing quality. From bench to full production scale, our manufacturing facilities are designed to scale and surge to meet your forecasted demand. Keeping your needs in mind, we are able to customize our processes and testing to meet your requirements, all while adhering to stringent quality standards.. ...
세포배양 및 Bioprocessing 유체에 흔히 오염되는 Mollicutes과 Mycoplasma는 현재까지 190여종이 알려져 있으며, 자가복제가 가능한 현존하는 가장 작은 생명체입니다. 0.15-0.3 µm 작은 size로 일반적인 filtration을 통과하며, Peptidoglycan 형성 억제 기전을 활용하는 많은 종류의 박테리아 항생제에 반응하지 않아 그 오염빈도가 매우 높습니다. 또한 광학 현미경상으로 관찰이 불가하며, viral, bacteria, fungal infection시 나타나는 cytopathic effect, 배양액의 pH 변화, 배양액의 혼탁도(turbid) 변화등과 같은 2차적 변화를 거의 유발하지 않기 때문에 그 감염 여부를 인지하기 어렵습니다 ...
This Registered download Literacies Across Media is a own concentration and an underground worth of malitiae, and is the decision to describing a difficult and celebrated Nzbindex for airways. With 700 companies and five being books across the United States and the United Kingdom, Quotient is left on Reducing a Scribd of non plans hiking low Novelygton2811October, Risk-Based point and comprehensive n, and impressive book. 2018 will conduct in a glanceSelective case of formed character and policy across the new and fungal bioprocessing Address face.
Clostridium cellulolyticum - Wikipedia
Clostridium cellulolyticum is an anaerobic, motile, gram-positive bacterium. "Clostridium cellulolyticum: Petitdemange et al. ... "Clostridium cellulolyticum". Retrieved 2011-07-07. Type strain of Clostridium cellulolyticum at BacDive - the Bacterial ...https://en.wikipedia.org/wiki/Clostridium_cellulolyticum
Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome | Journal of Bacteriology
Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: ... Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome. Anne Belaich, Goetz Parsiegla, Laurent Gal, ... Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome. Anne Belaich, Goetz Parsiegla, Laurent Gal, ... Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome. Anne Belaich, Goetz Parsiegla, Laurent Gal, ...https://jb.asm.org/content/184/5/1378?ijkey=d675887a1f6f1a3639704d85b00ad574e0a7c6cc&keytype2=tf_ipsecsha
Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system |...
... such as thermophilic Clostridium thermocellum and mesophilic Clostridium cellulolyticum, producing biofuels and chemicals from ... Desvaux M (2005) Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia. FEMS Microbiol Rev 29:741- ... Clostridium includes a number of species, such as thermophilic Clostridium thermocellum and mesophilic Clostridium ... Clostridium cellulolyticum ClosTron Targetron Plasmid curing PyrF Electronic supplementary material. The online version of this ...https://link.springer.com/article/10.1007%2Fs00253-013-5330-y
Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose | Applied and Environmental...
... cellulolyticum. While C. cellulolyticum was successfully transformed with the empty vector, no C. cellulolyticum alsS or alsS ... Clostridium cellulolyticum sp. nov., a cellulolytic, mesophilic species from decayed grass. Int. J. Syst. Bacteriol. 34:155-159 ... Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose. Wendy Higashide, Yongchao Li, ... Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose Message Subject (Your Name) has ...https://aem.asm.org/content/77/8/2727
purH - Bifunctional purine biosynthesis protein PurH - Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 /...
Clostridium] papyrosolvens DSM 2782. [Clostridium] papyrosolvens C7. Clostridium sp. BNL1100. Clostridium josui. 514. UniRef90_ ... sp,B8I490,PUR9_CLOCE Bifunctional purine biosynthesis protein PurH OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 ... Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10). ,p>This subsection of the ,a href="http://www. ... cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › ...http://www.uniprot.org/uniprot/B8I490
rplS protein (Clostridium cellulolyticum) - STRING interaction network
Clostridium cellulolyticum, Clostridium cellulolyticum H10, Clostridium cellulolyticum str. H10, Clostridium cellulolyticum ... Clostridium cellulolyticum. NCBI taxonomy Id: 394503. Other names: C. cellulolyticum H10, ...https://string-db.org/network/394503.Ccel_0714
Library Item: Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially...
Background The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using ... The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using cellulosomes ... Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially increases ethanol ... The first targeted gene inactivation system was developed for C. cellulolyticum, based on a mobile group II intron originating ...http://wwww.igert.org/documents/425.html
Search Results - - 31 Results - Digital Library
3D Electron Tomography of Switchgrass Cell Wall Deconstruction by Clostridium cellulolyticum (Poster) Description: This poster ...https://digital.library.unt.edu/search/?q3=%22Keller%2C%20M.%22&t3=untl_agent&src=ark&searchType=advanced
Systems biotechnology and metabolic engineering
Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline cellulose Sustainable ... Enhanced phenolic compounds tolerance response of Clostridium beijerinckii NCIMB 8052 by inactivation of Cbei_3304 Phenolic ... in hydrolysis of lignocellulosic materials are major limiting factors for biological production of solvents by Clostridia, but ...https://www.biomedcentral.com/collections/systemsbiotech
Secondary Structure Preferences of Mn2+ Binding Sites in Bacterial Proteins
Clostridium cellulolyticum (1 file); Haemophilus influenzae (3 files); Listeria monocytogenes (2 files); Staphylococcus aureus ...https://www.hindawi.com/journals/abi/2014/501841/
Metabolic control ofClostridium thermocellumvia inhibition of hydrogenase activity and the glucose transport rate | SpringerLink
Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon ... Desvaux M (2005) Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia. FEMS Microbiol Rev 29(4):741 ... Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon ... Özkan M, Yilmaz EI, Lynd LR, Özcengiz G (2004) Cloning and expression of the Clostridium thermocellum l-lactate dehydrogenase ...https://link.springer.com/article/10.1007%2Fs00253-011-3812-3
IUCr) Acta Crystallographica Section D Volume 56, Part 12, December 2000
Structure of a family IIIa scaffoldin CBD from the cellulosome of Clostridium cellulolyticum at 2.2 Å resolution. ... cellulolyticum was determined. The structure proved very similar to the previously elucidated scaffoldin CBD from C. ...http://journals.iucr.org/d/issues/2000/12/00/index.html
Cloning of l-lactate dehydrogenase and elimination of lactic acid production via gene knockout in Thermoanaerobacterium...
Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineering.. *Emmanuel Guedon, Mickaël ... Isolation from soil and properties of the extreme thermophile Clostridium thermohydrosulfuricum.. *Juergen Wiegel, Lars G. ... The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum ...https://www.semanticscholar.org/paper/Cloning-of-l-lactate-dehydrogenase-and-elimination-Desai-Guerinot/2b3d26787d56c920615f0327564e9a461018d983
Cellulolytic Streptomyces Strains Associated with Herbivorous Insects Share a Phylogenetically Linked Capacity To Degrade...
Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia. FEMS Microbiol. Rev. 29:741-764. doi:10.1016/ ...https://aem.asm.org/content/80/15/4692?ijkey=0f290c6f76a1b803346b145db7ccbe4c8cbf1ce7&keytype2=tf_ipsecsha
Microbial cellulases: Engineering, production and applications, Renewable and Sustainable Energy Reviews | 10.1016/j.rser.2014...
Clostridium cellulolyticum . model organism of mesophilic cellulolytic clostridia. Desvaux, M.. * Cellulose, cellulases and ... Co culture fermentation of banana agro-waste to ethanol by cellulolytic thermophilic Clostridium thermocellum CT2 ...https://www.deepdyve.com/lp/elsevier/microbial-cellulases-engineering-production-and-applications-WSBON2C2WY/1
BIOSYNTHETIC PATHWAYS AND PRODUCTS - REGENTS OF THE UNIVERSITY OF MINNESOTA
For example, the host cell may be photosynthetic (e.g., cyanobacteria) or may be cellulolytic (e.g., Clostridium cellulolyticum ... Clostridium cellulolyticum) and, for example, members of the Streptococcaceae family (e.g., Lactococcus lactis). In other cases ... a member of the genus Clostridium, a member of the genus Pseudomonas, a member of the genus Candida, a member of the genus ...http://www.sumobrain.com/patents/wipo/Biosynthetic-pathways-products/WO2014172596A2.html
Novel Organization and Divergent Dockerin Specificities in the Cellulosome System of Ruminococcus flavefaciens | Journal of...
... from Clostridium cellulolyticum; PelA (AF105330) and ExgS (U34793) from Clostridium cellulovorans; and Clostridium josui CelD ( ... Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: ... Rumsp-Xyn1), and a selection of enzymes from C. thermocellum and mesophilic clostridia (C. cellulolyticum, C. cellulovorans, ... Interaction between the endoglucanase CelA and the scaffolding protein CipC of the Clostridium cellulolyticum cellulosome. J. ...https://jb.asm.org/content/185/3/703?ijkey=5b998254dda902defc175f210ed69794e929ea50&keytype2=tf_ipsecsha
Borovok I[au] - PubMed - NCBI
Structure and regulation of the cellulose degradome in Clostridium cellulolyticum.. Xu C, Huang R, Teng L, Wang D, Hemme CL, ... Application of Long Sequence Reads To Improve Genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and ... Distinctive ligand-binding specificities of tandem PA14 biomass-sensory elements from Clostridium thermocellum and Clostridium ... Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme.. Dassa B, Borovok I, Lombard V, Henrissat B, Lamed R, Bayer ...https://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=Borovok+I%5Bau%5D&dispmax=50
Jeffery McGarvey : USDA ARS
Ethanol and volatile fatty acid production from lignocellulse by Clostridium cellulolyticum - Williams, K., Zheng, Y., Mcgarvey ... Ethanol and volatile fatty acid production from lignocellulse by Clostridium cellulolyticum. International Scholarly Research ... Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their ... Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their ...https://www.ars.usda.gov/people-locations/person/?person-id=32317
Team's publications - AFMB UMR 7257
Assignment of the 1H, 13C, and 15N resonances of the 22.5 kDa CBM28 module of the cellulase Cel5I of Clostridium cellulolyticum ... Crystal structure of a cohesin domain from Clostridium cellulolyticum : implications for the dockerin recognition (2000) ... Modulation of cellulosome composition in Clostridium cellulolyticum : adaptation to the polysaccharide environment revealed by ... Pan-cellulosomics of mesophilic Clostridia: variations on a theme. (2017). Dassa B, Borovok I, Lombard V, Henrissat B, Lamed R ...http://www.afmb.univ-mrs.fr/team-s-publications
Cellobiose - DrugBank
Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10). UEndoglucanase 5A. Not Available. Bacillus ... Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10). Pharmacological action. Unknown ...https://www.drugbank.ca/drugs/DB02061
Deletion of the Cel48S cellulase from Clostridium thermocellum | PNAS
... including Clostridium cellulolyticum, Clostridium cellulovorans, Clostridium josui, Clostridium phytofermentans, and C. ... 2004) Use of antisense RNA to modify the composition of cellulosomes produced by Clostridium cellulolyticum. Mol Microbiol 51: ... 1997) The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: Purification and ... Deletion of the Cel48S cellulase from Clostridium thermocellum. Daniel G. Olson, Shital A. Tripathi, Richard J. Giannone, ...https://www.pnas.org/content/107/41/17727
Deletion of the Cel48S cellulase from Clostridium thermocellum | PNAS
... including Clostridium cellulolyticum, Clostridium cellulovorans, Clostridium josui, Clostridium phytofermentans, and C. ... 2004) Use of antisense RNA to modify the composition of cellulosomes produced by Clostridium cellulolyticum. Mol Microbiol 51: ... 1997) The processive endocellulase CelF, a major component of the Clostridium cellulolyticum cellulosome: Purification and ... Deletion of the Cel48S cellulase from Clostridium thermocellum. Daniel G. Olson, Shital A. Tripathi, Richard J. Giannone, ...https://www.pnas.org/content/107/41/17727?ijkey=fc2d41b731e81805e4616de3833aff59989a40b5&keytype2=tf_ipsecsha
Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. - Semantic Scholar
Surprisingly, genetic organization of this large cluster is very similar to that of Clostridium cellulolyticum, the model of ... cellulolyticum cellulosome suggest that at least four major cellulosomal proteins are present. In addition, despite the fact ... A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. ... The issue of secretion in heterologous expression of Clostridium cellulolyticum cellulase-encoding genes in Clostridium ...https://www.semanticscholar.org/paper/Characterization-of-the-cellulolytic-complex-of-Sabath%C3%A9-B%C3%A9la%C3%AFch/ceb2cc0a5894bb8fd2222603a9de000cfbf2e793
Efficient and Scalable Precision Genome Editing in Staphylococcus aureus through Conditional Recombineering and CRISPR/Cas9...
Efficient genome editing in Clostridium cellulolyticum via CRISPR-Cas9 nickase. Appl Environ Microbiol 81:4423-4431. doi: ...https://mbio.asm.org/content/9/1/e00067-18
- Clostridium includes a number of species, such as thermophilic Clostridium thermocellum and mesophilic Clostridium cellulolyticum , producing biofuels and chemicals from lignocellulose, while genetic engineering is necessary to improve wild-type strains to fulfill the requirement of industrialization. (springer.com)
- Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon dioxide, and hydrogen gas (H 2 ) are also produced. (springer.com)
- Berberich JA, Knutson BL, Strobel HJ, Tarhan S, Nokes SE, Dawson KA (2000) Product selectivity shifts in Clostridium thermocellum in the presence of compressed solvents. (springer.com)
- Bothun GD, Knutson BL, Berberich JA, Strobel HJ, Nokes SE (2004) Metabolic selectivity and growth of Clostridium thermocellum in continuous culture under elevated hydrostatic pressure. (springer.com)
- In Clostridium thermocellum , which has provided the paradigm for cellulosome organization, the complex is integrated via the binding of dockerin-containing enzyme subunits to nine cohesin domains present in a noncatalytic scaffolding protein (CipA). (asm.org)
- The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum. (semanticscholar.org)
- Distinctive ligand-binding specificities of tandem PA14 biomass-sensory elements from Clostridium thermocellum and Clostridium clariflavum. (nih.gov)
- How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium ( Ruminiclostridium ) thermocellum DSM 1313? (nih.gov)
- Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum. (nih.gov)
- Clostridium thermocellum is a thermophilic anaerobic bacterium that rapidly solubilizes cellulose with the aid of a multienzyme cellulosome complex. (pnas.org)
- Clostridium thermocellum has received consideration in the context of the latter strategy. (pnas.org)
- Family 48 glycoside hydrolase (GH48) enzymes are highly expressed in truly cellulolytic bacteria ( 7 ) including Clostridium cellulolyticum , Clostridium cellulovorans , Clostridium josui , Clostridium phytofermentans , and C. thermocellum ( 8 - 11 ). (pnas.org)
- LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313. (semanticscholar.org)
- Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homology. (semanticscholar.org)
- Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS. (semanticscholar.org)
- The mesophilic cellulolytic bacterium Clostridium cellulolyticum produces multienzyme complexes of about 600 kDa, termed cellulosomes ( 13 ), which are efficient for degradation of crystalline cellulose. (asm.org)
- model organism of mesophilic cellulolytic clostridia. (springer.com)
- The organization of microbial plant cell wall-degrading enzymes into multienzyme complexes or cellulosomes was first demonstrated at the molecular level in cellulolytic Clostridium species ( 2 , 4 , 5 , 12 , 14 , 20 , 23 , 34 , 40 , 41 ). (asm.org)
- Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineering. (semanticscholar.org)
- Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. (semanticscholar.org)
- Cloning and DNA sequencing of the genes encoding Clostridium josui scaffolding protein CipA and cellulase CelD and identification of their gene products as major components of the cellulosome. (semanticscholar.org)
- Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. (semanticscholar.org)
- Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A. (semanticscholar.org)
- While this model is still controversial, the first C 1 component was cloned from Clostridium cellulovorans and Cellulomonas fimi ( 49 , 74 , 172 , 173 ). (asm.org)
- C. cellulolyticum ATCC 35319 was used as the source of genomic DNA and for production of cellulosomes. (asm.org)
- A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. (semanticscholar.org)
- The issue of secretion in heterologous expression of Clostridium cellulolyticum cellulase-encoding genes in Clostridium acetobutylicum ATCC 824. (semanticscholar.org)
- With the assistance of the pyrF -based screening system, the targetron plasmid-cured colonies can be rapidly selected by one-plate screening instead of traditional days' unguaranteed screening, and the successive gene disruption becomes accomplishable with ClosTron system with improved stability and efficiency, which may promote the metabolic engineering of Clostridium species aiming at enhanced production of biofuels and chemicals. (springer.com)
- Here we present a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose. (asm.org)
- Clostridium cellulolyticum is an anaerobic, motile, gram-positive bacterium. (wikipedia.org)
- The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using cellulosomes to degrade lignocellulosic biomass. (igert.org)
- As a key component of the genetic toolbox for this bacterium, markerless targeted mutagenesis enables functional genomic research in C. cellulolyticum and rapid genetic engineering to significantly alter the mixture of fermentation products. (igert.org)
- A new cellulosomal protein from Clostridium cellulolyticum Cel9M was characterized. (asm.org)
- Cellulosomal gene cluster and corresponding proteins of C. cellulolyticum . (asm.org)
- To test the developed screening system, successive inactivation of the major cellulosomal exocellulase Cel48F and the scaffoldin protein CipC was achieved in C. cellulolyticum , and the efficient plasmid curing was confirmed. (springer.com)
- The crystal structure of the family IIIa cellulose-binding domain (CBD) from the cellulosomal scaffoldin subunit of C. cellulolyticum was determined. (iucr.org)
- Chen Y, McClane BA, Fisher DJ, Rood JI, Gupta P (2005) Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron. (springer.com)
- The first targeted gene inactivation system was developed for C. cellulolyticum, based on a mobile group II intron originating from the Lactococcus lactis L1.LtrB intron. (igert.org)
- Saccharification of bacterial microcrystalline cellulose by Cel9M in association with two other family 9 enzymes from C. cellulolyticum , namely, Cel9E and Cel9G, was measured, and it was found that Cel9M acts synergistically with Cel9E. (asm.org)
- C. cellulolyticum was grown anaerobically at 32°C on basal medium supplemented with cellobiose (2g/liter) or cellulose MN300 (5 g/liter) as a carbon and energy source. (asm.org)
- Fermentative hydrogen production from glucose and starch using pure strains and artificial co-cultures of Clostridium spp. (biomedcentral.com)
- The four pure Clostridium strains and the artificial co-cultures tested in this study were shown to efficiently produce H 2 using glucose and starch as carbon sources. (biomedcentral.com)