Clostridium cellulolyticum
Clostridium
Cellulose
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Cellulase
Cellulosomes
Cellobiose
Cellulases
A family of glycosidases that hydrolyse crystalline CELLULOSE into soluble sugar molecules. Within this family there are a variety of enzyme subtypes with differing substrate specificities that must work together to bring about complete cellulose hydrolysis. They are found in structures called CELLULOSOMES.
Biomass
Clostridium difficile
Fermentation
Clostridium acetobutylicum
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Clostridium botulinum
A species of anaerobic, gram-positive, rod-shaped bacteria in the family Clostridiaceae that produces proteins with characteristic neurotoxicity. It is the etiologic agent of BOTULISM in humans, wild fowl, HORSES; and CATTLE. Seven subtypes (sometimes called antigenic types, or strains) exist, each producing a different botulinum toxin (BOTULINUM TOXINS). The organism and its spores are widely distributed in nature.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Carboxymethylcellulose Sodium
Libraries, Digital
Questionnaires
Professional Autonomy
Access to Information
Journal Impact Factor
Bibliometrics
Publishing
Peer Review, Research
The evaluation by experts of the quality and pertinence of research or research proposals of other experts in the same field. Peer review is used by editors in deciding which submissions warrant publication, by granting agencies to determine which proposals should be funded, and by academic institutions in tenure decisions.
Algorithms
Hydrogenase
Clostridium thermocellum
Hydrogen
The first chemical element in the periodic table. It has the atomic symbol H, atomic number 1, and atomic weight [1.00784; 1.00811]. It exists, under normal conditions, as a colorless, odorless, tasteless, diatomic gas. Hydrogen ions are PROTONS. Besides the common H1 isotope, hydrogen exists as the stable isotope DEUTERIUM and the unstable, radioactive isotope TRITIUM.
Glucose
Crystallization
Crystallography, X-Ray
X-Ray Diffraction
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Colicins
Ribosome Inactivating Proteins
N-Glycosidases that remove adenines from RIBOSOMAL RNA, depurinating the conserved alpha-sarcin loop of 28S RIBOSOMAL RNA. They often consist of a toxic A subunit and a binding lectin B subunit. They may be considered as PROTEIN SYNTHESIS INHIBITORS. They are found in many PLANTS and have cytotoxic and antiviral activity.
Ribosome Inactivating Proteins, Type 1
Ribosome Inactivating Proteins, Type 2
Encyclopedias as Topic
Glucans
Structural insights into the mechanism of formation of cellulosomes probed by small angle X-ray scattering. (1/23)
Exploring the mechanism by which the multiprotein complexes of cellulolytic organisms, the cellulosomes, attain their exceptional synergy is a challenge for biologists. We have studied the solution structures of the Clostridium cellulolyticum cellulosomal enzyme Cel48F in the free and complexed states with cohesins from Clostridium thermocellum and Clostridium cellulolyticum by small angle x-ray scattering in order to investigate the conformational events likely to occur upon complexation. The solution structure of the free cellulase indicates that the dockerin module is folded, whereas the linker connecting the catalytic module to the dockerin is extended and flexible. Remarkably, the docking of the different cohesins onto Cel48F leads to a pleating of the linker. The global structure determined here allowed modeling of the atomic structure of the C. cellulolyticum dockerin-cohesin interface, highlighting the local differences between both organisms responsible for the species specificity. (+info)Action of designer cellulosomes on homogeneous versus complex substrates: controlled incorporation of three distinct enzymes into a defined trifunctional scaffoldin. (2/23)
In recent work, we reported the self-assembly of a comprehensive set of defined "bifunctional" chimeric cellulosomes. Each complex contained the following: (i) a chimeric scaffoldin possessing a cellulose-binding module and two cohesins of divergent specificity and (ii) two cellulases, each bearing a dockerin complementary to one of the divergent cohesins. This approach allowed the controlled integration of desired enzymes into a multiprotein complex of predetermined stoichiometry and topology. The observed enhanced synergy on recalcitrant substrates by the bifunctional designer cellulosomes was ascribed to two major factors: substrate targeting and proximity of the two catalytic components. In the present work, the capacity of the previously described chimeric cellulosomes was amplified by developing a third divergent cohesin-dockerin device. The resultant trifunctional designer cellulosomes were assayed on homogeneous and complex substrates (microcrystalline cellulose and straw, respectively) and found to be considerably more active than the corresponding free enzyme or bifunctional systems. The results indicate that the synergy between two prominent cellulosomal enzymes (from the family-48 and -9 glycoside hydrolases) plays a crucial role during the degradation of cellulose by cellulosomes and that one dominant family-48 processive endoglucanase per complex is sufficient to achieve optimal levels of synergistic activity. Furthermore cooperation within a cellulosome chimera between cellulases and a hemicellulase from different microorganisms was achieved, leading to a trifunctional complex with enhanced activity on a complex substrate. (+info)Molecular cloning and transcriptional and expression analysis of engO, encoding a new noncellulosomal family 9 enzyme, from Clostridium cellulovorans. (3/23)
Clostridium cellulovorans produces a major noncellulosomal family 9 endoglucanase EngO. A genomic DNA fragment (40 kb) containing engO and neighboring genes was cloned. The nucleotide sequence contained reading frames for endoglucanase EngO, a putative response regulator, and a putative sensor histidine kinase protein. The engO gene consists of 2,172 bp and encodes a protein of 724 amino acids with a molecular weight of 79,474. Northern hybridizations revealed that the engO gene is transcribed as a monocistronic 2.6-kb mRNA. 5' RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE) PCR analysis indicated that the single transcriptional start site of engO was located 264 bp upstream from the first nucleotide of the translation initiation codon. Alignment of the engO promoter region provided evidence for highly conserved sequences that exhibited strong similarity to the sigma(A) consensus promoter sequences of gram-positive bacteria. EngO contains a typical N-terminal signal peptide of 28 amino acid residues, followed by a 149-amino-acid sequence which is homologous to the family 4-9 carbohydrate-binding domain. Downstream of this domain was an immunoglobulin-like domain of 89 amino acids. The C terminus contains a family 9 catalytic domain of glycosyl hydrolase. Mass spectrometry analysis of EngO was in agreement with that deduced from the nucleotide sequence. Expression of engO mRNA increased from early to middle exponential phase and decreased during the early stationary phase. EngO was highly active toward carboxymethyl cellulose but showed no activity towards xylan. It was optimally active at 40 to 50 degrees C and pH 5 to 6. The analysis of the products from the cellulose hydrolysis through thin-layer chromatography indicated its endoglucanase activity. (+info)Structural basis of cellulosome efficiency explored by small angle X-ray scattering. (4/23)
Cellulose, the main structural component of plant cell walls, is the most abundant carbohydrate polymer in nature. To break down plant cell walls, anaerobic microorganisms have evolved a large extracellular enzyme complex termed cellulosome. This megadalton catalytic machinery organizes an enzymatic assembly, tenaciously bound to a scaffolding protein via specialized intermodular "cohesin-dockerin" interactions that serve to enhance synergistic activity among the different catalytic subunits. Here, we report the solution structure properties of cellulosome-like assemblies analyzed by small angle x-ray scattering and molecular dynamics. The atomic models, generated by our strategy for the free chimeric scaffoldin and for binary and ternary complexes, reveal the existence of various conformations due to intrinsic structural flexibility with no, or only coincidental, inter-cohesin interactions. These results provide primary evidence concerning the mechanisms by which these protein assemblies attain their remarkable synergy. The data suggest that the motional freedom of the scaffoldin allows precise positioning of the complexed enzymes according to the topography of the substrate, whereas short-scale motions permitted by residual flexibility of the enzyme linkers allow "fine-tuning" of individual catalytic domains. (+info)Transcriptional analysis of the cip-cel gene cluster from Clostridium cellulolyticum. (5/23)
Twelve genes encoding key components of Clostridium cellulolyticum cellulosomes are clustered. Among them, the first, second, and fifth genes encode the assembly factor CipC and the two major cellulases Cel48F and Cel9E, respectively. Cellulolytic clones were selected from the noncellulolytic cipC insertional mutant trans-complemented with a cipC expression vector, in which one homologous recombination event between the 3' end of the chromosomal cipC gene and the plasmidic cipC gene has restored the cluster continuity. The absence of the enzymes encoded by the cluster in the cipC mutant was thus only due to a strong polar effect, indicating that all genes were transcriptionally linked. Two large transcripts were detected in cellulose-grown cells by Northern hybridization: a 14-kb messenger which carries the cipC-cel48F-cel8C-cel9G-cel9E coding sequences and, in a smaller amount, a 12-kb messenger which carries the genes located in the 3' part of the cluster. Four smaller transcripts were found in large amounts: a cipC-cel48F bicistronic one and three monocistronic ones, cipC, cel48F, and cel9E. The cipC-cel48F and cel48F messengers were shown to be stable. Analysis by reverse transcription-PCR suggested transcriptional linkage of all of the open reading frames. The production of a primary very large transcript covering the entire cluster was hypothesized. Primer extension analysis has identified two putative transcriptional start sites located 638/637 and 194 nucleotides upstream of the cipC translational start. The processing of the primary transcript would lead to the production of several secondary messengers displaying different stabilities, contributing to fine tuning of expression of individual genes of the operon. (+info)Enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum explored by two-dimensional analysis: identification of seven genes encoding new dockerin-containing proteins. (6/23)
The enzyme diversity of the cellulolytic system produced by Clostridium cellulolyticum grown on crystalline cellulose as a sole carbon and energy source was explored by two-dimensional electrophoresis. The cellulolytic system of C. cellulolyticum is composed of at least 30 dockerin-containing proteins (designated cellulosomal proteins) and 30 noncellulosomal components. Most of the known cellulosomal proteins, including CipC, Cel48F, Cel8C, Cel9G, Cel9E, Man5K, Cel9M, and Cel5A, were identified by using two-dimensional Western blot analysis with specific antibodies, whereas Cel5N, Cel9J, and Cel44O were identified by using N-terminal sequencing. Unknown enzymes having carboxymethyl cellulase or xylanase activities were detected by zymogram analysis of two-dimensional gels. Some of these enzymes were identified by N-terminal sequencing as homologs of proteins listed in the NCBI database. Using Trap-Dock PCR and DNA walking, seven genes encoding new dockerin-containing proteins were cloned and sequenced. Some of these genes are clustered. Enzymes encoded by these genes belong to glycoside hydrolase families GH2, GH9, GH10, GH26, GH27, and GH59. Except for members of family GH9, which contains only cellulases, the new modular glycoside hydrolases discovered in this work could be involved in the degradation of different hemicellulosic substrates, such as xylan or galactomannan. (+info)Incorporation of fungal cellulases in bacterial minicellulosomes yields viable, synergistically acting cellulolytic complexes. (7/23)
Artificial designer minicellulosomes comprise a chimeric scaffoldin that displays an optional cellulose-binding module (CBM) and bacterial cohesins from divergent species which bind strongly to enzymes engineered to bear complementary dockerins. Incorporation of cellulosomal cellulases from Clostridium cellulolyticum into minicellulosomes leads to artificial complexes with enhanced activity on crystalline cellulose, due to enzyme proximity and substrate targeting induced by the scaffoldin-borne CBM. In the present study, a bacterial dockerin was appended to the family 6 fungal cellulase Cel6A, produced by Neocallimastix patriciarum, for subsequent incorporation into minicellulosomes in combination with various cellulosomal cellulases from C. cellulolyticum. The binding of the fungal Cel6A with a bacterial family 5 endoglucanase onto chimeric miniscaffoldins had no impact on their activity toward crystalline cellulose. Replacement of the bacterial family 5 enzyme with homologous endoglucanase Cel5D from N. patriciarum bearing a clostridial dockerin gave similar results. In contrast, enzyme pairs comprising the fungal Cel6A and bacterial family 9 endoglucanases were substantially stimulated (up to 2.6-fold) by complexation on chimeric scaffoldins, compared to the free-enzyme system. Incorporation of enzyme pairs including Cel6A and a processive bacterial cellulase generally induced lower stimulation levels. Enhanced activity on crystalline cellulose appeared to result from either proximity or CBM effects alone but never from both simultaneously, unlike minicellulosomes composed exclusively of bacterial cellulases. The present study is the first demonstration that viable designer minicellulosomes can be produced that include (i) free (noncellulosomal) enzymes, (ii) fungal enzymes combined with bacterial enzymes, and (iii) a type (family 6) of cellulase never known to occur in natural cellulosomes. (+info)Evolution of acetoclastic methanogenesis in Methanosarcina via horizontal gene transfer from cellulolytic Clostridia. (8/23)
Phylogenetic analysis confirmed that two genes required for acetoclastic methanogenesis, ackA and pta, were horizontally transferred to the ancestor of Methanosarcina from a derived cellulolytic organism in the class Clostridia. This event likely occurred within the last 475 million years, causing profound changes in planetary methane biogeochemistry. (+info)
Combining free and aggregated cellulolytic systems in the cellulosome-producing bacterium Ruminiclostridium cellulolyticum |...
Regulation of cel genes of C. cellulolyticum: identification of GlyR2, a transcriptional regulator regulating cel5D gene...
Cellulase 9A from Clostridium cellulolyticum, Recombinant(EC 3.2.1.4) - Creative Enzymes
Sequence Similarity
- 1G9J: X-TAL STRUCTURE OF THE MUTANT E44Q OF THE CELLULASE CEL48F IN COMPLEX WITH A...
Glycoside Hydrolase Family 48 - CAZypedia
Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by...
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The enzyme diversity from the cellulolytic system produced by grown on - bcl-2 family members and the mitochondria in apoptosis
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Rhamnogalacturonan endolyase
Pagès S, Valette O, Abdou L, Bélaïch A, Bélaïch JP (August 2003). "A rhamnogalacturonan lyase in the Clostridium cellulolyticum ...
Butanol fuel
A strain of Clostridium cellulolyticum, a native cellulose-degrading microbe, affords isobutanol directly from cellulose. A ... Butanol can be produced by fermentation of biomass by the A.B.E. process using Clostridium acetobutylicum, Clostridium ... Anaerobic bacteria such as Clostridium acetobutylicum and Clostridium saccharobutylicum also contain these pathways. Succinate ... "Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose". Applied and Environmental ...
Cellulase
For example, Clostridium cellulolyticum produces 13 GH9 modular cellulases containing a different number and arrangement of ... of all family-9 glycoside hydrolases synthesized by the cellulosome-producing bacterium Clostridium cellulolyticum". The ... "A major new component in the cellulosome of Clostridium thermocellum is a processive endo-beta-1,4-glucanase producing ...
Acetoacetate decarboxylase
Bacillus polymyxa Chromobacterium violaceum Clostridium beijerinckii Clostridium cellulolyticum Pseudomonas putida While this ... Acetoacetate decarboxylase from Clostridium acetobutylicum catalyzes the decarboxylation of acetoacetate to yield acetone and ... In 1916, biochemist and future first president of Israel Chaim Weizmann was the first to isolate Clostridium acetobutylicum, a ... Acetoacetate decarboxylase has been found and studied in the following bacteria in addition to Clostridium acetobutylicum: ...
Cellulosome
Clostridium acetobutylicum Clostridium cellulolyticum Clostridium cellulovorans Clostridium clariflavum Clostridium josui ... The scaffoldin of some cellulosomes, an example being that of Clostridium thermocellum, contains a carbohydrate-binding module ... Dockerin Organelle Bayer, EA; Kenig, R; Lamed, R (1983). "Adherence of Clostridium thermocellum to cellulose". J. Bacteriol. ... Clostridium papyrosolvens Clostridium thermocellum (treated as model organism in cellulose utilization and also anaerobic ...
List of MeSH codes (B03)
Clostridium butyricum MeSH B03.300.390.400.200.200 - Clostridium cellulolyticum MeSH B03.300.390.400.200.205 - Clostridium ... Clostridium butyricum MeSH B03.510.415.400.200.200 - Clostridium cellulolyticum MeSH B03.510.415.400.200.205 - Clostridium ... Clostridium symbiosum MeSH B03.300.390.400.200.722 - Clostridium tertium MeSH B03.300.390.400.200.725 - Clostridium tetani MeSH ... Clostridium symbiosum MeSH B03.510.415.400.200.722 - Clostridium tertium MeSH B03.510.415.400.200.725 - Clostridium tetani MeSH ...
Clostridium
... cellulolyticum Clostridium cellulosi Clostridium cellulovorans Clostridium chartatabidum Clostridium chauvoei ... Clostridium aceticum Clostridium acetireducens Clostridium acetobutylicum Clostridium acidisoli Clostridium aciditolerans ... Clostridium acidurici Clostridium aerotolerans Clostridium aestuarii Clostridium akagii Clostridium aldenense Clostridium ... difficile Clostridium diolis Clostridium disporicum Clostridium drakei Clostridium durum Clostridium estertheticum Clostridium ...
Clostridium cellulolyticum
... is an anaerobic, motile, gram-positive bacterium. "Clostridium cellulolyticum: Petitdemange et al. ... "Clostridium cellulolyticum". Retrieved 2011-07-07. Type strain of Clostridium cellulolyticum at BacDive - the Bacterial ...
List of long species names
nov.; Reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100- ... 2018, Hungateiclostridium cellulolyticum (Patel et al. 1980) Zhang et al. 2018, Hungateiclostridium aldrichii (Yang et al. 1990 ... Originally described as Clostridium glycyrrhizinilyticum (31 letters), it jumped into this list upon being reclassified in ... It was originally named Clostridium thermosulfurigenes (29 letters), but later became one of the longest scientific names upon ...
Clostridium cellulolyticum - Wikipedia
RCSB PDB - 1G87: THE CRYSTAL STRUCTURE OF ENDOGLUCANASE 9G FROM CLOSTRIDIUM CELLULOLYTICUM
The high-resolution X-ray crystal structure of a multidomain endoglucanase from Clostridium cellulolyticum has been determined ... THE CRYSTAL STRUCTURE OF ENDOGLUCANASE 9G FROM CLOSTRIDIUM CELLULOLYTICUM. *DOI: 10.2210/pdb1G87/pdb ... X-Ray Crystal Structure of the Multidomain Endoglucanase Cel9G from Clostridium cellulolyticum Complexed with Natural and ... Ruminiclostridium cellulolyticum. Mutation(s): 0 Gene Names: celCCG. EC: 3.2.1.4. Find proteins for P37700 (Ruminiclostridium ...
Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system |...
... such as thermophilic Clostridium thermocellum and mesophilic Clostridium cellulolyticum, producing biofuels and chemicals from ... Desvaux M (2005) Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia. FEMS Microbiol Rev 29:741- ... Clostridium includes a number of species, such as thermophilic Clostridium thermocellum and mesophilic Clostridium ... Clostridium cellulolyticum ClosTron Targetron Plasmid curing PyrF Electronic supplementary material. The online version of this ...
Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome | Journal of Bacteriology
Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: ... Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome. Anne Belaich, Goetz Parsiegla, Laurent Gal, ... Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome. Anne Belaich, Goetz Parsiegla, Laurent Gal, ... Cel9M, a New Family 9 Cellulase of the Clostridium cellulolyticum Cellulosome. Anne Belaich, Goetz Parsiegla, Laurent Gal, ...
Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose | Applied and Environmental...
... cellulolyticum. While C. cellulolyticum was successfully transformed with the empty vector, no C. cellulolyticum alsS or alsS ... Clostridium cellulolyticum sp. nov., a cellulolytic, mesophilic species from decayed grass. Int. J. Syst. Bacteriol. 34:155-159 ... Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose. Wendy Higashide, Yongchao Li, ... Metabolic Engineering of Clostridium cellulolyticum for Production of Isobutanol from Cellulose Message Subject (Your Name) has ...
purH - Bifunctional purine biosynthesis protein PurH - Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 /...
Clostridium] papyrosolvens DSM 2782. [Clostridium] papyrosolvens C7. Clostridium sp. BNL1100. Clostridium josui. 514. UniRef90_ ... sp,B8I490,PUR9_CLOCE Bifunctional purine biosynthesis protein PurH OS=Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 ... Clostridium cellulolyticum (strain ATCC 35319 / DSM 5812 / JCM 6584 / H10). ,p>This subsection of the ,a href="http://www. ... cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Clostridia › Clostridiales › Ruminococcaceae › ...
The Issue of Secretion in Heterologous Expression of Clostridium cellulolyticum Cellulase-Encoding Genes in Clostridium...
The Issue of Secretion in Heterologous Expression of Clostridium cellulolyticum Cellulase-Encoding Genes in Clostridium ... The Issue of Secretion in Heterologous Expression of Clostridium cellulolyticum Cellulase-Encoding Genes in Clostridium ... The Issue of Secretion in Heterologous Expression of Clostridium cellulolyticum Cellulase-Encoding Genes in Clostridium ... The Issue of Secretion in Heterologous Expression of Clostridium cellulolyticum Cellulase-Encoding Genes in Clostridium ...
Investigation of stress tolerance of endoglucanases of the cellulosomes of Clostridium cellulolyticum to ethanol
|...
Investigation of stress tolerance of endoglucanases of the cellulosomes of Clostridium cellulolyticum to ethanol * U Anieto ... Keywords: Ethanol, Clostridium cellulolyticum carboxymethyl cellulose, endoglucanase activity, cellulosome Abstract. Current ... The hydrolytic function of crude cellulosome extracts from Clostridium cellulolyticum on carboxymethyl cellulose (CMC) with 0, ... A major obstacle in this pursuit is poor ethanol tolerance among cellulolytic Clostridium species. The objective of this work ...
Cellulase 9A from Clostridium cellulolyticum, Recombinant(EC 3.2.1.4) - Creative Enzymes
rplS protein (Clostridium cellulolyticum) - STRING interaction network
Recombinant Clostridium cellulolyticum 30S ribosomal protein S21(rpsU) - Cusabio
Domain combinations for 51445,51011,63446 superfamilies in Clostridium cellulolyticum H10
63446 in Clostridium cellulolyticum H10. Domain architectures illustrate each occurrence of 51445,51011,63446. ... Domain combinations for 51445,51011,63446 superfamilies in Clostridium cellulolyticum H10. The selected domain combination is ... 2 sequences contain the 51445,51011,63446 domain architecture in Clostridium cellulolyticum H10. ...
α-Galactosidase from Clostridium cellulolyticum, Recombinant(EC 3.2.1.22) - Creative Enzymes
Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase...
Clostridium thermocellum; Cste, Clostridium stercorarium; Ccel, Clostridium cellulolyticum; Tfus, Thermomonospora fusca; Csac, ... Clostridium phytofermentans has a repertoire of 108 putative glycoside hydrolases to break down cellulose and hemicellulose ... Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase ... Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase ...
Systems biotechnology and metabolic engineering
Metabolic engineering of Clostridium cellulolyticum for the production of n-butanol from crystalline cellulose Sustainable ... Enhanced phenolic compounds tolerance response of Clostridium beijerinckii NCIMB 8052 by inactivation of Cbei_3304 Phenolic ... in hydrolysis of lignocellulosic materials are major limiting factors for biological production of solvents by Clostridia, but ...
Search Results - - 31 Results - Digital Library
Secondary Structure Preferences of Mn2+ Binding Sites in Bacterial Proteins
Library Item: Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially...
Background The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using ... The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using cellulosomes ... Combined inactivation of the Clostridium cellulolyticum lactate and malate dehydrogenase genes substantially increases ethanol ... The first targeted gene inactivation system was developed for C. cellulolyticum, based on a mobile group II intron originating ...
Flux Analysis of the Metabolism of Clostridium cellulolyticum Grown in Cellulose-Fed Continuous Culture on a Chemically Defined...
... mesophilic bacterium Clostridium cellulolyticum was performed in cellulose-fed chemostat cultures with ammonium as the growth- ... While the use of cellobiose allows highlighting of metabolic limitation and regulation of C. cellulolyticum under ammonium- ... Flux Analysis of the Metabolism of Clostridium cellulolyticum Grown in Cellulose-Fed Continuous Culture on a Chemically Defined ... Flux Analysis of the Metabolism of Clostridium cellulolyticum Grown in Cellulose-Fed Continuous Culture on a Chemically Defined ...
Cthe 2147 - Glycoside hydrolase family 5 precursor - Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 /...
Clostridium sp. Bc-iso-3. 660. UPI0001E2E6A5. Hungateiclostridium cellulolyticum. 654. UPI0003B81F83. CLOTM ... Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium ... Clostridium thermocellum (strain ATCC 27405 / DSM 1237 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) (Ruminiclostridium ... cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Clostridia › Clostridiales › Hungateiclostridiaceae › ...
Metabolic control ofClostridium thermocellumvia inhibition of hydrogenase activity and the glucose transport rate | SpringerLink
Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon ... Desvaux M (2005) Clostridium cellulolyticum: model organism of mesophilic cellulolytic clostridia. FEMS Microbiol Rev 29(4):741 ... Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon ... Özkan M, Yilmaz EI, Lynd LR, Özcengiz G (2004) Cloning and expression of the Clostridium thermocellum l-lactate dehydrogenase ...
IUCr) Acta Crystallographica Section D Volume 56, Part 12, December 2000
Borovok I[au] - PubMed - NCBI
Structure and regulation of the cellulose degradome in Clostridium cellulolyticum.. Xu C, Huang R, Teng L, Wang D, Hemme CL, ... Application of Long Sequence Reads To Improve Genomes for Clostridium thermocellum AD2, Clostridium thermocellum LQRI, and ... Distinctive ligand-binding specificities of tandem PA14 biomass-sensory elements from Clostridium thermocellum and Clostridium ... Pan-Cellulosomics of Mesophilic Clostridia: Variations on a Theme.. Dassa B, Borovok I, Lombard V, Henrissat B, Lamed R, Bayer ...
Jeffery McGarvey : USDA ARS
Ethanol and volatile fatty acid production from lignocellulse by Clostridium cellulolyticum - Williams, K., Zheng, Y., Mcgarvey ... Ethanol and volatile fatty acid production from lignocellulse by Clostridium cellulolyticum. International Scholarly Research ... Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their ... Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their ...
Diverse specificity of cellulosome attachment to the bacterial cell surface | Scientific Reports
The Clostridium cellulolyticum dockerin displays a dual binding mode for its cohesin partner. J. Biol. Chem. 283, 18422-18430 ( ... In Clostridium thermocellum (Ct), cellulosome assembly occurs through the binding of type-I Cohs within the primary scaffoldin ... These are reminiscent of the highly symmetrical Coh-binding sites in type-I Docs from C. thermocellum and other clostridia. It ... Clostridium thermocellum complexes. The crystal structure of the CtCohScaC2-XDocCipB complex was solved by molecular ...
Novel Organization and Divergent Dockerin Specificities in the Cellulosome System of Ruminococcus flavefaciens | Journal of...
... from Clostridium cellulolyticum; PelA (AF105330) and ExgS (U34793) from Clostridium cellulovorans; and Clostridium josui CelD ( ... Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: ... Rumsp-Xyn1), and a selection of enzymes from C. thermocellum and mesophilic clostridia (C. cellulolyticum, C. cellulovorans, ... Interaction between the endoglucanase CelA and the scaffolding protein CipC of the Clostridium cellulolyticum cellulosome. J. ...
Rhamnogalacturonan endolyase - Wikipedia
Bug Creates Butanol Directly from Cellulose | MIT Technology Review
The key was adding Liaos sugar-to-isobutanol pathway to a microbe, Clostridium cellulolyticum, that likes chewing on biomass ... Super bug: Genetic engineers reprogrammed this microbe, Clostridium cellulolyticum, to turn cellulose into butanol.. Now James ... The next step is to move the genetic modifications to a faster-growing variant of Clostridium or some other microbe. Liao bets ...
Cellulase - Wikipedia
For example, Clostridium cellulolyticum produces 13 GH9 modular cellulases containing a different number and arrangement of ... of all family-9 glycoside hydrolases synthesized by the cellulosome-producing bacterium Clostridium cellulolyticum". The ... "A major new component in the cellulosome of Clostridium thermocellum is a processive endo-beta-1,4-glucanase producing ...
Microbial cellulases: Engineering, production and applications, Renewable and Sustainable Energy Reviews | 10.1016/j.rser.2014...
CellulosomeATCCCellulaseAcetobutylicumStrain of Clostridium cellulolyticumCellulolytic clostridiaBacterium Clostridium cellulolyticumSpeciesRecombinantBacillusOrganismMesophilic cellulolytic bacteriumThermophilicMetabolicEndoglucanaseStrainsSubstrateBacteriaCellulovoransAdding Liao'sPhytofermentansProteinPerfringensBiomassStercorariumCellulosomalGenusScaffoldinAgrobacteriumSporeMobile group II intBiofuel productionGenesInactivationDifficileGeneticCellobiosePlasmidBeijerinckiiEnzymesNickaseOperonEndoglucanasesDSM1313Ethanol productionCellulose degradation
Cellulosome8
- The hydrolytic function of crude cellulosome extracts from Clostridium cellulolyticum on carboxymethyl cellulose (CMC) with 0, 5, 10, 15, 20 and 25% ethanol was determined. (ajol.info)
- How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium ( Ruminiclostridium ) thermocellum DSM 1313? (nih.gov)
- In Clostridium thermocellum , which has provided the paradigm for cellulosome organization, the complex is integrated via the binding of dockerin-containing enzyme subunits to nine cohesin domains present in a noncatalytic scaffolding protein (CipA). (asm.org)
- Clostridium thermocellum is a thermophilic anaerobic bacterium that rapidly solubilizes cellulose with the aid of a multienzyme cellulosome complex. (pnas.org)
- Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. (semanticscholar.org)
- Cloning and DNA sequencing of the genes encoding Clostridium josui scaffolding protein CipA and cellulase CelD and identification of their gene products as major components of the cellulosome. (semanticscholar.org)
- Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS. (semanticscholar.org)
- Dockerin type I repeat (IPR002105) Gram-positive, thermophilic anaerobes such as Clostridium thermocellum or Clostridium cellulolyticum secretes a highly active and thermostable cellulase complex (cellulosome) resp. (ebi.ac.uk)
ATCC3
- C. cellulolyticum ATCC 35319 was used as the source of genomic DNA and for production of cellulosomes. (asm.org)
- A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. (semanticscholar.org)
- We report here that Clostridium thermocellum ATCC 27405 enters non-growth states in response to specific growth conditions. (biomedcentral.com)
Cellulase2
- Letter to the Editor: Assignment of the 1H, 13C, and 15N resonances of the 22,5 kDa CBM28 module of the cellulase Cel5I of Clostridium cellulolyticum" J. Biomol. (wisc.edu)
- Cellulase, clostridia, and ethanol. (semanticscholar.org)
Acetobutylicum2
- White and black boxes symbolize the cel48F (from C. cellulolyticum ) and cel48A (from C. acetobutylicum ) genes, respectively. (asm.org)
- Thermostable xylanase10B from Clostridium acetobutylicum ATCC824. (biofueljournal.com)
Strain of Clostridium cellulolyticum1
- Building on earlier work at UCLA in creating a synthetic pathway for isobutanol production , the BESC researchers managed to achieve such a single-step process by developing a strain of Clostridium cellulolyticum, a native cellulose-degrading microbe that could synthesize isobutanol directly from cellulose. (innovationtoronto.com)
Cellulolytic clostridia1
- model organism of mesophilic cellulolytic clostridia. (springer.com)
Bacterium Clostridium cellulolyticum3
- The mesophilic cellulolytic bacterium Clostridium cellulolyticum produces multienzyme complexes of about 600 kDa, termed cellulosomes ( 13 ), which are efficient for degradation of crystalline cellulose. (asm.org)
- The model bacterium Clostridium cellulolyticum efficiently degrades crystalline cellulose and hemicellulose, using cellulosomes to degrade lignocellulosic biomass. (igert.org)
- An investigation of cellulose degradation by the nonruminal, cellulolytic, mesophilic bacterium Clostridium cellulolyticum was performed in cellulose-fed chemostat cultures with ammonium as the growth-limiting nutrient. (inrae.fr)
Species8
- Clostridium includes a number of species, such as thermophilic Clostridium thermocellum and mesophilic Clostridium cellulolyticum , producing biofuels and chemicals from lignocellulose, while genetic engineering is necessary to improve wild-type strains to fulfill the requirement of industrialization. (springer.com)
- With the assistance of the pyrF -based screening system, the targetron plasmid-cured colonies can be rapidly selected by one-plate screening instead of traditional days' unguaranteed screening, and the successive gene disruption becomes accomplishable with ClosTron system with improved stability and efficiency, which may promote the metabolic engineering of Clostridium species aiming at enhanced production of biofuels and chemicals. (springer.com)
- A major obstacle in this pursuit is poor ethanol tolerance among cellulolytic Clostridium species. (ajol.info)
- The organization of microbial plant cell wall-degrading enzymes into multienzyme complexes or cellulosomes was first demonstrated at the molecular level in cellulolytic Clostridium species ( 2 , 4 , 5 , 12 , 14 , 20 , 23 , 34 , 40 , 41 ). (asm.org)
- Species-specificity of the cohesin-dockerin interaction between Clostridium thermocellum and Clostridium cellulolyticum: prediction of specificity determinants of the dockerin domain. (ebi.ac.uk)
- As demonstrated by the scattered phyletic distribution of neurotoxin-producing clostridia [ 10 ] and the patterns of sequence similarity between different neurotoxin gene clusters [ 11 ], CNT genes appear to have undergone significant lateral transfer between different species of Clostridium . (biomedcentral.com)
- While CNTs have undergone frequent lateral transfer between species of Clostridium , no CNT homologues have been identified outside of the Clostridium genus. (biomedcentral.com)
- Genome-scale modeling and simulations have been guided for metabolic engineering of Clostridium species for the production of organic solvents and organic acids. (cyberleninka.org)
Recombinant1
- The pdc and adh genes from Z. mobilis were cloned in pNW33N, and transformed to Clostridium thermocellum DSM 1313 by electroporation to generate recombinant CTH- pdc, CTH- adh and CTH- pdc-adh strains that carried heterologous pdc , adh , and both genes, respectively. (biomedcentral.com)
Bacillus5
- Bacillus, Clostridium. (docme.ru)
- Thuricin CD is a two-component bacteriocin produced by Bacillus thuringiensis that kills a wide range of clinically significant Clostridium difficile . (plos.org)
- The two thuricin CD peptides act synergistically, exhibiting a narrow spectrum of activity targeting mainly the spore forming genera - Bacillus and Clostridium . (plos.org)
- Schematic structure of a Bacillus / Clostridium spore. (asmscience.org)
- Bacterial spores are produced by Gram-positive bacteria including members of the Bacillus and Clostridium genera, and are widely understood to be dormant cell forms that remain viable for long periods of time until growth conditions become favorable. (biomedcentral.com)
Organism3
- Clostridium phytofermentans has a repertoire of 108 putative glycoside hydrolases to break down cellulose and hemicellulose into sugars, which this organism then ferments primarily to ethanol. (nih.gov)
- Plasmid transformation, random mutagenesis and heterologous expression systems have previously been developed for C. cellulolyticum, but targeted mutagenesis has not been reported for this organism, hindering genetic engineering. (igert.org)
- Like all clostridia, this organism forms terminal endospores, which confer a high degree of resistance to heat, desiccation and other environmental challenges. (biomedcentral.com)
Mesophilic cellulolytic bacterium1
- Clostridium cellulolyticum is a mesophilic cellulolytic bacterium. (up.ac.za)
Thermophilic2
- Clostridium thermocellum is an anaerobic thermophilic bacterium with cellulolytic property and the ability to produce ethanol. (biomedcentral.com)
- Clostridium thermocellum is an anaerobic thermophilic bacterium that exhibits high levels of cellulose solublization and produces ethanol as an end product of its metabolism. (biomedcentral.com)
Metabolic4
- Here we present a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose. (asm.org)
- While the use of cellobiose allows highlighting of metabolic limitation and regulation of C. cellulolyticum under ammonium-limited conditions, some of these events should then rather be interpreted as distortions of the metabolism. (inrae.fr)
- Bothun GD, Knutson BL, Berberich JA, Strobel HJ, Nokes SE (2004) Metabolic selectivity and growth of Clostridium thermocellum in continuous culture under elevated hydrostatic pressure. (springer.com)
- Improvement of cellulolytic properties of Clostridium cellulolyticum by metabolic engineering. (semanticscholar.org)
Endoglucanase3
- The high-resolution X-ray crystal structure of a multidomain endoglucanase from Clostridium cellulolyticum has been determined at a 1.6-A resolution. (rcsb.org)
- The crystal structure of endoglucanase CelA, a family 8 glycosyl hydrolase from Clostridium thermocellum. (expasy.org)
- Three-dimensional structures are available for a very large number of Family GH5 enzymes, the first solved being that of the Clostridium thermocellum endoglucanase CelC [ 11 ]. (cazypedia.org)
Strains4
- Fermentative hydrogen production from glucose and starch using pure strains and artificial co-cultures of Clostridium spp. (biomedcentral.com)
- Interestingly, all the Clostridium strains and co-cultures were shown to utilize lactate (present in a starch-containing medium), and C. beijerinckii was able to re-consume formate producing additional H 2 . (biomedcentral.com)
- The four pure Clostridium strains and the artificial co-cultures tested in this study were shown to efficiently produce H 2 using glucose and starch as carbon sources. (biomedcentral.com)
- Gram-negative bacteria such as Escherichia coli , Klebsiella oxytoca , Z. mobilis, Gram-positive bacteria such as Clostridium cellulolyticum , Lactobacillus casei and several yeast strains have been engineered for bioethanol production from cellulosic substrates. (niscair.res.in)
Substrate3
- More recently, transcriptome analysis of C. cellulolyticum cultured on different substrates [ 10 ] revealed substrate specificity of carbohydrate active enzymes (CAZymes) and the transcriptional regulation of core cellulases by Carbon Catabolite Repression (CCR). (biomedcentral.com)
- Fluorescent in situ hybridization and quantitative PCR showed that when particulate MN301 cellulose was used as sole substrate, most Clostridium cells were found adhered to cellulose particles in suspension, while most Geobacter cells were attached to the electrode. (iwaponline.com)
- Results showed clostridia varied in H2 production, substrate degradation, biomass accumulation, and fermentation product distribution. (fastkpdf.com)
Bacteria2
- Family 48 glycoside hydrolase (GH48) enzymes are highly expressed in truly cellulolytic bacteria ( 7 ) including Clostridium cellulolyticum , Clostridium cellulovorans , Clostridium josui , Clostridium phytofermentans , and C. thermocellum ( 8 - 11 ). (pnas.org)
- The cellulose-degrading community, targeted by analysis of the glycoside hydrolase families 5 ( cel5 ) and 48 ( cel48 ), showed a dominance of bacteria belonging the Firmicutes and Bacteriodetes, and a positive correlation was found between the cellulose degradation rate of wheat straw with the level of C. cellulolyticum . (biomedcentral.com)
Cellulovorans3
- While this model is still controversial, the first C 1 component was cloned from Clostridium cellulovorans and Cellulomonas fimi ( 49 , 74 , 172 , 173 ). (asm.org)
- Primary sequence analysis of Clostridium cellulovorans cellulose binding protein A. (semanticscholar.org)
- Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A. (semanticscholar.org)
Adding Liao's1
- The key was adding Liao's sugar-to-isobutanol pathway to a microbe, Clostridium cellulolyticum, that likes chewing on biomass but does not normally make butanol. (technologyreview.com)
Phytofermentans1
- Targeted gene inactivation in Clostridium phytofermentans shows that cellulose degradation requires the family 9 hydrolase Cphy3367. (nih.gov)
Protein4
- To test the developed screening system, successive inactivation of the major cellulosomal exocellulase Cel48F and the scaffoldin protein CipC was achieved in C. cellulolyticum , and the efficient plasmid curing was confirmed. (springer.com)
- A new cellulosomal protein from Clostridium cellulolyticum Cel9M was characterized. (asm.org)
- Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homology. (semanticscholar.org)
- Despite considerable progress in understanding CNT structure and function, the evolutionary origins of CNTs remain a mystery as they are unique to Clostridium and possess a sequence and structural architecture distinct from other protein families. (biomedcentral.com)
Perfringens2
- Chen Y, McClane BA, Fisher DJ, Rood JI, Gupta P (2005) Construction of an alpha toxin gene knockout mutant of Clostridium perfringens type A by use of a mobile group II intron. (springer.com)
- B1UZL4(Cas9 coding gene from Clostridium perfringens D str. (addgene.org)
Biomass2
- Clostridium thermocellum has the ability to catabolize cellulosic biomass into ethanol, but acetic acid, lactic acid, carbon dioxide, and hydrogen gas (H 2 ) are also produced. (springer.com)
- Distinctive ligand-binding specificities of tandem PA14 biomass-sensory elements from Clostridium thermocellum and Clostridium clariflavum. (nih.gov)
Stercorarium1
- EC 3.2.1.176) - Clostridium cellulolyticum CelF, Clostridium stercorarium CelY, and Clostridium thermocellum CelS - to create a diverse library of Cel48 enzymes with an average of 106 mutations from the closest native enzyme. (caltech.edu)
Cellulosomal2
Genus1
- Genus Clostridium Prazmowski 1880, 23 AL . (microbiologyresearch.org)
Scaffoldin1
- Revisiting the Regulation of the Primary Scaffoldin Gene in Clostridium thermocellum. (nih.gov)
Agrobacterium2
- The overall fold of the subunit proved to be similar to those of the D-tagatose 3-epimerase from Pseudomonas cichorii and the D-psicose 3-epimerases from Agrobacterium tumefaciens and Clostridium cellulolyticum. (rcsb.org)
- We wanted to produce a library of mutants from one of the five D-psicose 3-epimerases (Dpe): two from Agrobacterium tumefaciens (UniProt ID Q7CVR7 and A9CH28), one from Clostrium cellulolyticum (UniProt ID B8I944), one from Flavonifractor plautii (UniProt ID G9YVF8) and one from Pseudomonas cichorii (UniProt ID O50580). (igem.org)
Spore1
- Clostridium termitidis CT1112 is an anaerobic, Gram-positive, mesophilic, spore-forming, cellulolytic bacterium, originally isolated from the gut of a wood feeding termite Nasusitermes lujae . (biomedcentral.com)
Mobile group II int1
- The first targeted gene inactivation system was developed for C. cellulolyticum, based on a mobile group II intron originating from the Lactococcus lactis L1.LtrB intron. (igert.org)
Biofuel production1
- Among them, Clostridium sticklandii is one of the potential organisms to be exploited as a microbial cell factory for biofuel production. (cyberleninka.org)
Genes2
- Light and dark gray boxes symbolize the cipC1 and orfX genes from C. cellulolyticum , respectively. (asm.org)
- Transcription and expression regulation of some individual cel genes (cel5A, cel5I, cel5D and cel44O) of Clostridium cellulolyticum were investigated. (nottingham.ac.uk)
Inactivation1
- The efficient intron-based gene inactivation system produced the first non-random, targeted mutations in C. cellulolyticum. (igert.org)
Difficile2
- This alternative approach stems from the fact that in the clinical setting the use of broad spectrum antimicrobials has lead to the emergence of multi-drug resistant pathogens and the observation that the disruption of the colonic microbiota through administration of broad spectrum antibiotic treatment predisposes susceptible individuals to infection by the nosocomial pathogen Clostridium difficile [5] , [6] . (plos.org)
- Interestingly thuricin CD kills a wide range of clinical C. difficile ribotypes commonly associated with Clostridium difficile associated diarrhoea (CDAD) including the hypervirulent strain B1/NAP1/027 [7] . (plos.org)
Genetic3
- As a key component of the genetic toolbox for this bacterium, markerless targeted mutagenesis enables functional genomic research in C. cellulolyticum and rapid genetic engineering to significantly alter the mixture of fermentation products. (igert.org)
- Genetic engineers reprogrammed this microbe, Clostridium cellulolyticum, to turn cellulose into butanol. (technologyreview.com)
- The next step is to move the genetic modifications to a faster-growing variant of Clostridium or some other microbe. (technologyreview.com)
Cellobiose1
- C. cellulolyticum was grown anaerobically at 32°C on basal medium supplemented with cellobiose (2g/liter) or cellulose MN300 (5 g/liter) as a carbon and energy source. (asm.org)
Plasmid1
- To solve this problem, a pyrF -based screening system was developed and implemented in C. cellulolyticum strain H10 in this study for efficient targetron plasmid curing. (springer.com)
Beijerinckii1
- Four pure Clostridium cultures, including C. butyricum CWBI1009, C. pasteurianum DSM525, C. beijerinckii DSM1820 and C. felsineum DSM749, and three different co-cultures composed of (1) C. pasteurianum and C. felsineum , (2) C. butyricum and C. felsineum , (3) C. butyricum and C. pasteurianum , were grown in 20 L batch bioreactors. (biomedcentral.com)
Enzymes1
- Saccharification of bacterial microcrystalline cellulose by Cel9M in association with two other family 9 enzymes from C. cellulolyticum , namely, Cel9E and Cel9G, was measured, and it was found that Cel9M acts synergistically with Cel9E. (asm.org)
Nickase1
- Efficient Genome Editing in Clostridium cellulolyticum via CRISPR-Cas9 Nickase. (naver.com)
Operon1
- Abdou L, Boileau C, de Philip P, Pages S, Fierobe HP, Tardif C (2008) Transcriptional regulation of the Clostridium cellulolyticum cip-cel operon: a complex mechanism involving a catabolite-responsive element. (springer.com)
Endoglucanases2
- Clostridium cellulolyticum endoglucanases C (celcCC). (expasy.org)
- Clostridium thermocellum endoglucanases A (celA). (expasy.org)
DSM13131
- LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313. (semanticscholar.org)
Ethanol production1
- The bifunctional alcohol and aldehyde dehydrogenase gene, adhE, is necessary for ethanol production in Clostridium thermocellum and Thermoanaerobacterium saccharolyticum. (semanticscholar.org)
Cellulose degradation1
- Cellulose-degradation by C. cellulolyticum has been extensively studied. (up.ac.za)