Clostridium beijerinckii
Clostridium
Acetone
Neocallimastix
Gram-Positive Asporogenous Rods, Irregular
Solvents
Fermentation
Clostridium difficile
Clostridium botulinum
Molecular Sequence Data
Maintenance of DeltapH by a butanol-tolerant mutant of Clostridium beijerinckii. (1/18)
The isolation of Clostridium beijerinckii mutants that are more tolerant of butanol than the wild-type offered the opportunity to investigate whether the membrane activities which are required for maintaining the transmembrane DeltapH (the difference in pH between the cellular interior and exterior) are sensitive targets of butanol toxicity. The DeltapH was measured by the accumulation of [14C]benzoate using late-exponential-phase cells which were suspended in citrate/phosphate buffer at pH 5 (to maximize the DeltapH component of the protonmotive force) and supplemented with glucose and Mg2+. The DeltapH of the butanol-tolerant tolerant mutant, strain BR54, of C. beijerinckii NCIMB 8052 was found to be significantly more tolerant of added butanol than the wild-type. Thus, in potassium citrate/phosphate buffer the mutant cells maintained a DeltapH of 1.4 when butanol was added to a concentration of 1.5 % (w/v), while the wild-type DeltapH was reduced to 0.1. The DeltapH of both strains was completely dissipated with 1.75 % butanol, an effect attributed to a chaotropic effect on the membrane phospholipids. Similar results were obtained in sodium citrate/phosphate buffer. In the absence of added Mg2+, the DeltapH of the mutant decreased in both sodium and potassium citrate/phosphate buffer, but more rapidly in the former. Interestingly, the addition of butanol at low concentrations (0.8 %) prevented this DeltapH dissipation, but only in cells suspended in sodium citrate/phosphate buffer, and not in potassium citrate/phosphate buffer. In wild-type cells the decrease in DeltapH occurred more slowly than in the mutant, and sparing of the DeltapH by 0.8 % butanol was less pronounced. The authors interpret these data to mean that the DeltapH is dissipated in the absence of Mg2+ by a Na+- or K+-linked process, possibly by a Na+/H+ or a K+/H+ antiporter, and that the former is inhibited by butanol. Apparently, butanol can selectively affect a membrane-associated function at concentrations lower than required for the complete dissipation of transmembrane ion gradients. Additionally, since the butanol-tolerant mutant BR54 is deficient in the ability to detoxify methylglyoxal (MG) and contains higher levels of MG than the wild-type, the higher Na+/H+ antiporter activity of the mutant may be due to the greater degree of protein glycation by MG in the mutant cells. The mechanism of butanol tolerance may be an indirect result of the elevated glycation of cell proteins in the mutant strain. Analysis of membrane protein fractions revealed that mutant cells contained significantly lower levels of unmodified arginine residues than those of the wild-type cells, and that unmodified arginine residues of the wild-type were decreased by exposure of the growing cells to added MG. (+info)Evidence for the presence of an alternative glucose transport system in Clostridium beijerinckii NCIMB 8052 and the solvent-hyperproducing mutant BA101. (2/18)
The effects of substrate analogs and energy inhibitors on glucose uptake and phosphorylation by Clostridium beijerinckii provide evidence for the operation of two uptake systems: a previously characterized phosphoenolpyruvate-dependent phosphotransferase system (PTS) and a non-PTS system probably energized by the transmembrane proton gradient. In both wild-type C. beijerinckii NCIMB 8052 and the butanol-hyperproducing mutant BA101, PTS activity declined at the end of exponential growth, while glucokinase activity increased in the later stages of fermentation. The non-PTS uptake system, together with enhanced glucokinase activity, may provide an explanation for the ability of the mutant to utilize glucose more effectively during fermentation despite the fact that it is partially defective in PTS activity. (+info)Influence of the protein environment on the redox potentials of flavodoxins from Clostridium beijerinckii. (3/18)
The flavin mononucleotide (FMN) quinones in flavodoxin have two characteristic redox potentials, namely, Em(FMNH./FMNH-) for the one-electron reduction of the protonated FMN (E1) and Em(FMN/FMNH.) for the proton-coupled one-electron reduction (E2). These redox potentials in native and mutant flavodoxins obtained from Clostridium beijerinckii were calculated by considering the protonation states of all titratable sites as well as the energy contributed at the pKa value of FMN during protonation at the N5 nitrogen (pKa(N5)). E1 is sensitive to the subtle differences in the protein environments in the proximity of FMN. The protein dielectric volume that prevents the solvation of charged FMN quinones is responsible for the downshift of 130-160 mV of the E1 values with respect to that in an aqueous solution. The influence of the negatively charged 5'-phosphate group of FMN quinone on E1 could result in a maximum shift of 90 mV. A dramatic difference of 130 mV in the calculated E2 values of FMN quinone of the native and G57T mutant flavodoxins is due to the difference in the pKa(N5) values. This is due to the difference in the influence exerted by the carbonyl group of the protein backbone at residue 57. (+info)Engineered synthetic pathway for isopropanol production in Escherichia coli. (4/18)
A synthetic pathway was engineered in Escherichia coli to produce isopropanol by expressing various combinations of genes from Clostridium acetobutylicum ATCC 824, E. coli K-12 MG1655, Clostridium beijerinckii NRRL B593, and Thermoanaerobacter brockii HTD4. The strain with the combination of C. acetobutylicum thl (acetyl-coenzyme A [CoA] acetyltransferase), E. coli atoAD (acetoacetyl-CoA transferase), C. acetobutylicum adc (acetoacetate decarboxylase), and C. beijerinckii adh (secondary alcohol dehydrogenase) achieved the highest titer. This strain produced 81.6 mM isopropanol in shake flasks with a yield of 43.5% (mol/mol) in the production phase. To our knowledge, this work is the first to produce isopropanol in E. coli, and the titer exceeded that from the native producers. (+info)Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 and the hyper-butanol-producing mutant BA101 during the shift from acidogenesis to solventogenesis. (5/18)
(+info)Fermentation of rice bran and defatted rice bran for butanol 5 production using clostridium beijerinckii NCIMB 8052. (6/18)
We examined butanol fermentation by Clostridium beijerinckii NCIMB 8052 using various hydrolyzates obtained from rice bran, which is one of the most abundant agricultural by-products in Korea and Japan. In order to increase the amount of fermentable sugars in the hydrolyzates of rice bran, various hydrolysis procedures were applied. Eight different hydrolyzates were prepared using rice bran (RB) and defatted rice bran (DRB) with enzyme or acid treatment or both. Each hydrolyzate was evaluated in terms of total sugar concentration and butanol production after fermentation by C. beijerinckii NCIMB 8052. Acid treatment yielded more sugar than enzyme treatment, and combined treatment with enzyme and acid yielded even more sugars as compared with single treatment with enzyme or acid. As a result, the highest sugar concentration (33 g/l) was observed from the hydrolyzate from DRB (100 g/l) with combined treatment using enzyme and acid. Prior to fermentation of the hydrolyzates, we examined the effect of P2 solution containing yeast extract, buffer, minerals, and vitamins on production of butanol during the fermentation. Fermentation of the hydrolyzates with or without addition of P2 was performed using C. beijerinckii NCIMB 8052 in a 1-l anaerobic bioreactor. Although the RB hydrolyzates were able to support growth and butanol production, addition of P2 solution into the hydrolyzates significantly improved cell growth and butanol production. The highest butanol production (12.24 g/l) was observed from the hydrolyzate of DRB with acid and enzyme treatment after supplementation of P2 solution. (+info)Large number of phosphotransferase genes in the Clostridium beijerinckii NCIMB 8052 genome and the study on their evolution. (7/18)
(+info)D-2,3-butanediol production due to heterologous expression of an acetoin reductase in Clostridium acetobutylicum. (8/18)
(+info)'Clostridium beijerinckii' is a species of gram-positive, spore-forming, rod-shaped bacteria found in various environments such as soil, aquatic sediments, and the intestinal tracts of animals. It is named after the Dutch microbiologist Martinus Willem Beijerinck.
This bacterium is capable of fermenting a wide range of organic compounds and producing a variety of metabolic end-products, including butanol, acetone, and ethanol. 'Clostridium beijerinckii' has attracted interest in biotechnology due to its potential for the production of biofuels and industrial chemicals through fermentation processes.
However, it is also known to cause food spoilage and, under certain circumstances, can produce harmful metabolites that may pose a risk to human health. Therefore, proper handling and safety precautions are necessary when working with this bacterium in laboratory or industrial settings.
Butanols are a family of alcohols with four carbon atoms and a chemical formula of C4H9OH. They are commonly used as solvents, intermediates in chemical synthesis, and fuel additives. The most common butanol is n-butanol (normal butanol), which has a straight chain of four carbon atoms. Other forms include secondary butanols (such as isobutanol) and tertiary butanols (such as tert-butanol). These compounds have different physical and chemical properties due to the differences in their molecular structure, but they all share the common characteristic of being alcohols with four carbon atoms.
'Clostridium' is a genus of gram-positive, rod-shaped bacteria that are widely distributed in nature, including in soil, water, and the gastrointestinal tracts of animals and humans. Many species of Clostridium are anaerobic, meaning they can grow and reproduce in environments with little or no oxygen. Some species of Clostridium are capable of producing toxins that can cause serious and sometimes life-threatening illnesses in humans and animals.
Some notable species of Clostridium include:
* Clostridium tetani, which causes tetanus (also known as lockjaw)
* Clostridium botulinum, which produces botulinum toxin, the most potent neurotoxin known and the cause of botulism
* Clostridium difficile, which can cause severe diarrhea and colitis, particularly in people who have recently taken antibiotics
* Clostridium perfringens, which can cause food poisoning and gas gangrene.
It is important to note that not all species of Clostridium are harmful, and some are even beneficial, such as those used in the production of certain fermented foods like sauerkraut and natto. However, due to their ability to produce toxins and cause illness, it is important to handle and dispose of materials contaminated with Clostridium species carefully, especially in healthcare settings.
Acetone is a colorless, volatile, and flammable liquid organic compound with the chemical formula (CH3)2CO. It is the simplest and smallest ketone, and its molecules consist of a carbonyl group linked to two methyl groups. Acetone occurs naturally in the human body and is produced as a byproduct of normal metabolic processes, particularly during fat burning.
In clinical settings, acetone can be measured in breath or blood to assess metabolic status, such as in cases of diabetic ketoacidosis, where an excess production of acetone and other ketones occurs due to insulin deficiency and high levels of fatty acid breakdown. High concentrations of acetone can lead to a sweet, fruity odor on the breath, often described as "fruity acetone" or "acetone breath."
Neocallimastix is a genus of anaerobic fungi that are commonly found in the digestive tracts of herbivorous mammals and birds, where they play a crucial role in breaking down complex plant material into simpler compounds that can be absorbed and utilized by their hosts. These fungi are characterized by their ability to produce enzymes that can break down cellulose, hemicellulose, and lignin, the major structural components of plant cell walls. Under a microscope, Neocallimastix species appear as branching, septate hyphae with rounded or pointed ends, and they reproduce by forming spores within specialized structures called sporangia.
"Gram-Positive Asporogenous Rods, Irregular" is a medical term used to describe a specific type of bacteria. Here's the breakdown:
1. **Gram-Positive**: This refers to the bacterium's reaction to the Gram stain test, a common laboratory method used to classify bacteria based on their cell wall structure. Gram-positive bacteria retain the crystal violet stain used in this test, appearing purple under the microscope.
2. **Asporogenous**: This term indicates that the bacterium does not form endospores, which are highly resistant structures that some bacteria create in response to harsh environmental conditions. Endospores are capable of surviving extreme conditions and can germinate into vegetative cells when conditions improve. Asporogenous bacteria lack this ability.
3. **Rods**: This term describes the bacterium's shape. Rod-shaped bacteria, also known as bacilli, are longer than they are wide.
4. **Irregular**: This modifier is used when the rods are not uniform in size and shape, meaning they may vary in length or width, or both.
So, a "Gram-Positive Asporogenous Rod, Irregular" is a type of bacteria that is gram-positive (stains purple with the Gram stain), does not form endospores (asporogenous), has a rod shape (bacilli), and exhibits irregularities in its size and/or shape. Examples of such bacteria might include certain species within the genera Corynebacterium, Listeria, or Rhodococcus.
Solvents, in a medical context, are substances that are capable of dissolving or dispersing other materials, often used in the preparation of medications and solutions. They are commonly organic chemicals that can liquefy various substances, making it possible to administer them in different forms, such as oral solutions, topical creams, or injectable drugs.
However, it is essential to recognize that solvents may pose health risks if mishandled or misused, particularly when they contain volatile organic compounds (VOCs). Prolonged exposure to these VOCs can lead to adverse health effects, including respiratory issues, neurological damage, and even cancer. Therefore, it is crucial to handle solvents with care and follow safety guidelines to minimize potential health hazards.
Fermentation is a metabolic process in which an organism converts carbohydrates into alcohol or organic acids using enzymes. In the absence of oxygen, certain bacteria, yeasts, and fungi convert sugars into carbon dioxide, hydrogen, and various end products, such as alcohol, lactic acid, or acetic acid. This process is commonly used in food production, such as in making bread, wine, and beer, as well as in industrial applications for the production of biofuels and chemicals.
'Clostridium difficile' (also known as 'C. difficile' or 'C. diff') is a type of Gram-positive, spore-forming bacterium that can be found in the environment, including in soil, water, and human and animal feces. It is a common cause of healthcare-associated infections, particularly in individuals who have recently received antibiotics or have other underlying health conditions that weaken their immune system.
C. difficile produces toxins that can cause a range of symptoms, from mild diarrhea to severe colitis (inflammation of the colon) and potentially life-threatening complications such as sepsis and toxic megacolon. The most common toxins produced by C. difficile are called TcdA and TcdB, which damage the lining of the intestine and cause inflammation.
C. difficile infections (CDIs) can be difficult to treat, particularly in severe cases or in patients who have recurrent infections. Treatment typically involves discontinuing any unnecessary antibiotics, if possible, and administering specific antibiotics that are effective against C. difficile, such as metronidazole, vancomycin, or fidaxomicin. In some cases, fecal microbiota transplantation (FMT) may be recommended as a last resort for patients with recurrent or severe CDIs who have not responded to other treatments.
Preventing the spread of C. difficile is critical in healthcare settings, and includes measures such as hand hygiene, contact precautions, environmental cleaning, and antibiotic stewardship programs that promote the appropriate use of antibiotics.
Clostridium infections are caused by bacteria of the genus Clostridium, which are gram-positive, rod-shaped, spore-forming, and often anaerobic organisms. These bacteria can be found in various environments, including soil, water, and the human gastrointestinal tract. Some Clostridium species can cause severe and potentially life-threatening infections in humans. Here are some of the most common Clostridium infections with their medical definitions:
1. Clostridioides difficile infection (CDI): An infection caused by the bacterium Clostridioides difficile, previously known as Clostridium difficile. It typically occurs after antibiotic use disrupts the normal gut microbiota, allowing C. difficile to overgrow and produce toxins that cause diarrhea, colitis, and other gastrointestinal symptoms. Severe cases can lead to sepsis, toxic megacolon, or even death.
2. Clostridium tetani infection: Also known as tetanus, this infection is caused by the bacterium Clostridium tetani. The spores of this bacterium are commonly found in soil and animal feces. They can enter the body through wounds, cuts, or punctures, germinate, and produce a potent exotoxin called tetanospasmin. This toxin causes muscle stiffness and spasms, particularly in the neck and jaw (lockjaw), which can lead to difficulty swallowing, breathing, and potentially fatal complications.
3. Clostridium botulinum infection: This infection is caused by the bacterium Clostridium botulinum and results in botulism, a rare but severe paralytic illness. The bacteria produce neurotoxins (botulinum toxins) that affect the nervous system, causing symptoms such as double vision, drooping eyelids, slurred speech, difficulty swallowing, dry mouth, and muscle weakness. In severe cases, botulism can lead to respiratory failure and death.
4. Gas gangrene (Clostridium perfringens infection): A rapidly progressing soft tissue infection caused by Clostridium perfringens or other clostridial species. The bacteria produce potent exotoxins that cause tissue destruction, gas production, and widespread necrosis. Gas gangrene is characterized by severe pain, swelling, discoloration, and a foul-smelling discharge. If left untreated, it can lead to sepsis, multi-organ failure, and death.
5. Clostridioides difficile infection (C. difficile infection): Although not caused by a typical clostridial species, C. difficile is a gram-positive, spore-forming bacterium that can cause severe diarrhea and colitis, particularly in hospitalized patients or those who have recently taken antibiotics. The bacteria produce toxins A and B, which damage the intestinal lining and contribute to inflammation and diarrhea. C. difficile infection can range from mild to life-threatening, with complications such as sepsis, toxic megacolon, and bowel perforation.
'Clostridium botulinum' is a gram-positive, rod-shaped, anaerobic bacteria that produces one or more neurotoxins known as botulinum toxins. These toxins are among the most potent naturally occurring biological poisons and can cause a severe form of food poisoning called botulism in humans and animals. Botulism is characterized by symmetrical descending flaccid paralysis, which can lead to respiratory and cardiovascular failure, and ultimately death if not treated promptly.
The bacteria are widely distributed in nature, particularly in soil, sediments, and the intestinal tracts of some animals. They can form spores that are highly resistant to heat, chemicals, and other environmental stresses, allowing them to survive for long periods in adverse conditions. The spores can germinate and produce vegetative cells and toxins when they encounter favorable conditions, such as anaerobic environments with appropriate nutrients.
Human botulism can occur through three main routes of exposure: foodborne, wound, and infant botulism. Foodborne botulism results from consuming contaminated food containing preformed toxins, while wound botulism occurs when the bacteria infect a wound and produce toxins in situ. Infant botulism is caused by the ingestion of spores that colonize the intestines and produce toxins, mainly affecting infants under one year of age.
Prevention measures include proper food handling, storage, and preparation practices, such as cooking and canning foods at appropriate temperatures and for sufficient durations. Wound care and prompt medical attention are crucial in preventing wound botulism. Vaccines and antitoxins are available for prophylaxis and treatment of botulism in high-risk individuals or in cases of confirmed exposure.
Molecular sequence data refers to the specific arrangement of molecules, most commonly nucleotides in DNA or RNA, or amino acids in proteins, that make up a biological macromolecule. This data is generated through laboratory techniques such as sequencing, and provides information about the exact order of the constituent molecules. This data is crucial in various fields of biology, including genetics, evolution, and molecular biology, allowing for comparisons between different organisms, identification of genetic variations, and studies of gene function and regulation.
Clostridium beijerinckii
Fusarium oxysporum f.sp. spinacia
Acetone-butanol-ethanol fermentation
Martinus Beijerinck
Clostridium acetobutylicum
Clostridium saccharobutylicum
Clostridium saccharoperbutylacetonicum
Peptide plane flipping
Butanol fuel
Martha L. Ludwig
Clostridium
Acetoacetate decarboxylase
List of Clostridium species
Lactate racemase
List of MeSH codes (B03)
Clostridium novyi-NT
Butyric acid
Solventogenesis
Entner-Doudoroff pathway
Clostridium beijerinckii - Wikipedia
Scholarship 16/23042-9 - Clostridium beijerinckii, Bagaço de cana-de-açúcar - BV FAPESP
Clostridium beijerinckii
MicrobiotaClostridium ⇒ Clostridium beijerinckii | Microbiota | MetaBiom
Evidence of mixotrophic carbon-capture by n-butanol-producer Clostridium beijerinckii | Documents - Universidad de Salamanca
Clostridium beijerinckii and Clostridium difficile detoxify methylglyoxal by a novel mechanism involving glycerol dehydrogenase...
1fln.1 | SWISS-MODEL Template Library
Publication : USDA ARS
Society for Mathematical Biology Annual Meeting 2012 - Poster Sessions
Molecules | Free Full-Text | Characterization of a (2R,3R)-2,3-Butanediol Dehydrogenase from Rhodococcus erythropolis WZ010
Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale | Nature Biotechnology
화학공학소재연구정보센터(CHERIC) | 연구정보 | 문헌DB | 학술지 검색
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Find Research outputs - Heriot-Watt Research Portal
Pesquisa | Biblioteca Virtual em Saúde
The Strain Game: From Basic Bacteria to Next Generation Probiotics
Clostridium ⇒ Clostridium methylpentosum | Microbiota | MetaBiom
Clostridium ⇒ Clostridium sartagoforme | Microbiota | MetaBiom
2FLV | Genus
Cybase
MicrobiotaClostridium ⇒ Clostridium cellulovorans | Microbiota | MetaBiom
Pre GI: BLASTP Hits
B6db families : 2 6 1 89
DIFE-250 | QuantiChrom™ Iron Assay Kit Biotrend
Pre GI: CDS description
Mari Chinn
Role of hydrogen bonding interactions to N(3)H of the flavin mononucleotide cofactor in the modulation of the redox potentials...
Enhancing electricity production in microbial fuel cells using defined co-cultures : WestminsterResearch
Photo-biohydrogen production potential of Rhodobacter capsulatus- PK from wheat straw | Biotechnology for Biofuels and...
80524
- In contrast to gram-negative bacteria, little is known about the mechanisms by which gram-positive bacteria degrade the toxic metabolic intermediate methylglyoxal (MG). Clostridium beijerinckii BR54, a Tn1545 insertion mutant of the NCIMB 8052 strain, formed cultures that contained significantly more (free) MG than wild-type cultures. (aber.ac.uk)
- Molecular characterization of a family of choline-binding proteins of Clostridium beijerinckii NCIB 8052. (bvsalud.org)
- Application of raw industrial sweetpotato hydrolysates for butanol production by Clostridium beijerinckii NCIMB 8052. (ncsu.edu)
- Fermentation of rice bran and defatted rice bran for butanol production using Clostridium beijerinckii NCIMB 8052. (bakrie.ac.id)
Acetobutylicum14
- Clostridium acetobutylicum Butanol A.B.E. process Clostridium beijerinckii information Archived 2007-11-22 at the Wayback Machine from JGI lpsn.dsmz.de, list of prokaryotic names with standing nomenclature Archived 2020-03-23 at the Wayback Machine. (wikipedia.org)
- In this process, acetone, butanol and ethanol are produced in a mixed fermentation (typically in a ratio of 3:6:1) by the solventogenic Clostridium species Clostridium acetobutylicum , Clostridium beijerinckii , Clostridium saccharobutylicum or Clostridium saccharoperbutylacetonicum from sugar or starch feedstocks 7 . (nature.com)
- Clostridium acetobutylicum has played an important role in biotechnology throughout the 20th century. (thecoffeeparlor.com)
- Edited by Mark Hower, student of Rachel Larsen and Kit Pogliano, From MicrobeWiki, the student-edited microbiology resource, "Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. (thecoffeeparlor.com)
- Clostridium acetobutylicum, which is also known as the 'Weizmann organism,' was … This is mainly because solvents and organic acids could be used for production of fine chemicals such as butyl butyrate, butyl oleate, etc. (thecoffeeparlor.com)
- Biological hydrogen production by Clostridium acetobutylicum in an unsaturated flow reactor. (thecoffeeparlor.com)
- Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. (thecoffeeparlor.com)
- 13) Gimenez, J.A. The genome of Clostridium acetobutylicum is 3.94088 Mega-base pairs long with a 192-kb megaplasmid. (thecoffeeparlor.com)
- Nitrogen-fixation genes and nitrogenase activity in Clostridium acetobutylicum and Clostridium beijerinckii. (thecoffeeparlor.com)
- George, and S.M. Williams and Wilkins, Baltimore, MD. Currently, there is a resurgence of interest in Clostridium acetobutylicum, the biocatalyst of the historical Weizmann process, to produce n-butanol for use both as a bulk chemical and as a renewable alternative transportation fuel. (thecoffeeparlor.com)
- Clostridium acetobutylicum is an organism historically used for. (thecoffeeparlor.com)
- In this study, production of butanol directly from hemicellulose was achieved simply through overexpression of an indigenous xylanase in Clostridium acetobutylicum. (thecoffeeparlor.com)
- Clostridium acetobutylicum" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings).Descriptors are arranged in a hierarchical structure, … It can only survive up to several hours in aerobic conditions, in which it will form endospores that can last for years even in aerobic conditions. (thecoffeeparlor.com)
- pH control has been essential for butanol production with Clostridium acetobutylicum . (springeropen.com)
Saccharoperbutylacetonicum3
- The study was conducted to evaluate fermentation by Clostridium thermocellum and C. saccharoperbutylacetonicum in a continuous-flow, high-solids reactor. (aimspress.com)
- Thang V, Kanda K, KobayanshI G (2010) Production of Acetone-Butanol-Ethanol (ABE) in direct fermentation of cassava by Clostridium saccharoperbutylacetonicum N1-4. (aimspress.com)
- Al-Shorgani NK, Kalil MS, Yusoff W (2011) The effect of different carbon sources on butanol production using Clostridium saccharoperbutylacetonicum N1-4. (aimspress.com)
Flavodoxin2
- Control of oxidation-reduction potentials in flavodoxin from Clostridium beijerinckii: the role of conformation changes. (expasy.org)
- In the flavodoxin from Clostridium beijerinckii, the γ-carboxylate group of glutamate-59 serves as a dual hydrogen bond acceptor with the N(3)H of flavin mononucleotide (FMN) cofactor and the amide hydrogen of the adjacent polypeptide backbone in all three oxidation states. (uky.edu)
Strain5
- Here, we sequenced and assembled the complete genome of the type strain Clostridium beijerinckii DSM 791T, composed of a circular chromosome and a circular megaplasmid, and used it for a comparison with other genomes to evaluate diversity and capture the evolution of the whole species. (fairdomhub.org)
- Using a combinatorial pathway library approach, we first mined a historical industrial strain collection for superior enzymes that we used to engineer the autotrophic acetogen Clostridium autoethanogenum . (nature.com)
- Clostridium is the genus, butyricum is the species, and WB-STR-0006 is actually the strain. (pendulumlife.com)
- Lastly, we have Clostridium beijerinckii , yet another novel, next generation beneficial strain available only in Pendulum probiotics. (pendulumlife.com)
- Characterization of Clostridium ljungdahlii OTA1: a non-autotrophic hyper ethanol-producing strain. (ncsu.edu)
Bacteria2
- The joint research proposal focuses on a sustainable, bio-based production of the important commodity chemical 1,3-propanediol (PDO) by anaerobic bacteria of the genus Clostridium. (fapesp.br)
- This episode: I talk with Dr. Walter Sandoval-Espinola, a researcher from Paraguay, now a postdoc at Harvard, about his discovery that biofuel-producing bacteria Clostridium beijerinckii can also transform CO 2 and carbon monoxide into biofuels! (bacteriofiles.com)
Species4
- The project brings comprehensive study of diversity in Clostridium beijerinckii, solvent-producing species with potential use in industrial biotechnology. (fairdomhub.org)
- The fasta file contains amino acid sequences of genes forming the accessory genome of the Clostridium beijerinckii species. (fairdomhub.org)
- We found that strains WB53 and HUN142 were misidentified and did not belong to the Clostridium beijerinckii species. (fairdomhub.org)
- Dorn-In S, Schwaiger K, Springer C, Barta L, Ulrich S, Gareis M. Development of a multiplex qPCR for the species identification of Clostridium estertheticum , C. frigoriphilum , C. bowmanii and C. tagluense -like from blown pack spoilage (BPS) meats and from wild boars. (dsmz.de)
Butyricum2
- For example, you'll see on our Butyricum bottle that we list Clostridium butyricum WB-STR-0006. (pendulumlife.com)
- Colleen explains that the (Pendulum) formulation is stable but needs to be refrigerated, and lists the five strains: Akkermansia muciniphila, Anaerobutyricum hallii, Bifidobacterium infantis, Clostridium butyricum, and Clostridium beijerinckii. (gladdenlongevity.com)
Bacterium2
- Clostridium beijerinckii is a gram positive, rod shaped, motile bacterium of the genus Clostridium. (wikipedia.org)
- Clostridium beijerinckii is a relatively widely studied, yet non-model, bacterium. (fairdomhub.org)
Acetone-butanol-ethanol1
- The economical viability of the biobutanol production process by ABE (acetone-butanol-ethanol) fermentation using Clostridium strains faces challenges such as high product inhibition, low yields and productivity, and high substrate costs. (fapesp.br)
Strains1
- The fasta file contains amino acid sequences of unique genes found in various Clostridium beijerinckii strains. (fairdomhub.org)
Metabolic2
- Metabolic Response of Clostridium ljungdahlii to Oxygen Exposure. (ncsu.edu)
- Here, we present the genome-scale metabolic network of Clostridium ljungdahlii, the first such model for an acetogen. (biomedcentral.com)
Autoethanogenum1
- Sequence data for Clostridium autoethanogenum using three generations of sequencing technologies. (ncsu.edu)
Genome1
- It is believed that present day Mollicutes (Eubacteria) have evolved regressively (i.e., by genome reduction) from gram-positive clostridia-like ancestors with a low GC content in DNA. (up.ac.za)
19801
- 2001", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Reclassification of Clostridium diolis Biebl and Spröer 2003 as a later heterotypic synonym of Clostridium beijerinckii Donker 1926 (Approved Lists 1980) emend. (go.jp)
Difficile1
- Inactivation of gldA in both C. beijerinckii and Clostridium difficile gave rise to pinpoint colonies that could not be subcultured, indicating that glycerol dehydrogenase performs an essential function in both organisms. (aber.ac.uk)
Production1
- The high toxicity of butanol constitutes a major bottleneck for high-level production of butanol with Clostridium spp. (biomedcentral.com)
Study1
- In the second study the best coculture combination was a mixture of Geobacter sulphurreducens, Clostridium beijerinckii and Saccharomyces cerevisiae giving a maximum power density of 80 mWm-2. (westminster.ac.uk)
Engineering in C1
- Multiplex genome engineering in Clostridium beijerinckii NCIMB 8052 using CRISPR-Cas12a. (nih.gov)
Genus1
- Clostridium beijerinckii is a gram positive, rod shaped, motile bacterium of the genus Clostridium. (wikipedia.org)
Butanol production2
- She will be presenting Functional genomics approach to identify new determinants for butanol production in Clostridium beijerinckii NCIMB 8052. (osu.edu)
- The results on different extraction methods of apple peels and the determination of their antimicrobial properties, the process development for butanol production using co-cultures of Clostridium beijerinckii and aerobic bacterium, sulfamethoxazole removal by non-specific peroxygenase immobilization, kinetics and upscaling potential and the effectiveness of vitamin B3 and D3 against UVB-induced photoaging on primary human dermal fibroblasts are exemplified. (bionanonet.net)
Newly isolated1
- The co-production of isopropanol with butanol by the newly isolated Clostridium sp. (biomedcentral.com)
Br212
- Specifically, Clostridium beijerinckii Br21 can produce 1,3-propanediol from glycerol, but it does not have the gene dhaB, responsible for the first step of the reaction. (fapesp.br)
- Thus, C. beijerinckii Br21 will be transformed for insertion of the gene dhaB, which should increase the reductive pathway of glycerol metabolism. (fapesp.br)
Gram positive1
- It is believed that present day Mollicutes (Eubacteria) have evolved regressively (i.e., by genome reduction) from gram-positive clostridia-like ancestors with a low GC content in DNA. (up.ac.za)
Solventogenic1
- One of the main obstacles preventing solventogenic clostridia from achieving higher yields in biofuel production is the toxicity of produced solvents. (nih.gov)
Pathway1
- In addition, this procedure will help to understand the actual biochemical pathway that C. beijerinckii is making use to form 1,3-propanediol, a totally non-explored task in current literature. (fapesp.br)
Gene1
- 11. Gene transcription repression in Clostridium beijerinckii using CRISPR-dCas9. (nih.gov)
Fermentation process1
- During fermentation process, the high ratio of Clostridium and low ratio of Bacillus composition indicated that this symbiotic system was an effective and easily controlled cultivation model for ABE fermentation under microaerobic conditions. (biomedcentral.com)
Dehydrogenase1
- With enhancement of buffering capacity and alcohol dehydrogenase activities, butanol and isopropanol titer by Clostridium sp. (biomedcentral.com)
Reduction1
- A co-culture of Shewanella oneidensis and Clostridium beijerinckii gave a maximum power density (Pmax) of 87 mWm-2 (67% COD reduction) compared to 60 mWm-2 for C.beijerinckii alone and 48 mWm-2 for S.oneidensis alone. (westminster.ac.uk)
Characterization1
- Molecular characterization of a family of choline-binding proteins of Clostridium beijerinckii NCIB 8052. (bvsalud.org)
Type1
- A novel wild-type Clostridium sp. (biomedcentral.com)