Clostridium acetobutylicum
Clostridium
Acetone
Clostridium difficile
Phosphate Acetyltransferase
Coenzyme A-Transferases
Clostridium beijerinckii
Fermentation
Phosphotransferases (Carboxyl Group Acceptor)
Rubredoxins
Gene Expression Regulation, Bacterial
Clostridium botulinum
A species of anaerobic, gram-positive, rod-shaped bacteria in the family Clostridiaceae that produces proteins with characteristic neurotoxicity. It is the etiologic agent of BOTULISM in humans, wild fowl, HORSES; and CATTLE. Seven subtypes (sometimes called antigenic types, or strains) exist, each producing a different botulinum toxin (BOTULINUM TOXINS). The organism and its spores are widely distributed in nature.
Clostridium butyricum
Cellulase
Hemerythrin
Hydrogenase
Spores, Bacterial
Acetate Kinase
Cellulosomes
Acetoin
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Solvents
Biofuels
Amino Acid Sequence
Butyrates
Enterocolitis, Pseudomembranous
An acute inflammation of the INTESTINAL MUCOSA that is characterized by the presence of pseudomembranes or plaques in the SMALL INTESTINE (pseudomembranous enteritis) and the LARGE INTESTINE (pseudomembranous colitis). It is commonly associated with antibiotic therapy and CLOSTRIDIUM DIFFICILE colonization.
Cloning, Molecular
Gene Knockout Techniques
Butyric Acid
Cellulose
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Culture Media
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
Alcohol Dehydrogenase
Acetyl-CoA C-Acetyltransferase
Base Sequence
Hydrogen-Ion Concentration
Escherichia coli
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Clostridium thermocellum
Clostridium cellulolyticum
Carboxy-Lyases
Restriction Mapping
RNA, Antisense
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.
Clostridium tetani
Plasmids
Alcohol Oxidoreductases
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
Operon
RNA, Bacterial
Acetates
Metabolic Networks and Pathways
Transcription, Genetic
Botulinum Toxins
Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.
Sequence Homology, Amino Acid
Clostridium sordellii
Ethanol
A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.
Bacterial Toxins
Genetic Engineering
Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and their implications for solvent formation. (1/98)
It has been suggested (L. H. Harris, R. P. Desai, N. E. Welker, and E. T. Papoutsakis, Biotechnol. Bioeng. 67:1-11, 2000) that butyryl phosphate (BuP) is a regulator of solventogenesis in Clostridium acetobutylicum. Here, we determined BuP and acetyl phosphate (AcP) levels in fermentations of C. acetobutylicum wild type (WT), degenerate strain M5, a butyrate kinase (buk) mutant, and a phosphotransacetylase (pta) mutant. A sensitive method was developed to measure BuP and AcP in the same sample. Compared to the WT, the buk mutant had higher levels of BuP and AcP; the BuP levels were high during the early exponential phase, and there was a peak corresponding to solvent production. Consistent with this, solvent formation was initiated significantly earlier and was much stronger in the buk mutant than in all other strains. For all strains, initiation of butanol formation corresponded to a BuP peak concentration that was more than 60 to 70 pmol/g (dry weight), and higher and sustained levels corresponded to higher butanol formation fluxes. The BuP levels never exceeded 40 to 50 pmol/g (dry weight) in strain M5, which produces no solvents. The BuP profiles were bimodal, and there was a second peak midway through solventogenesis that corresponded to carboxylic acid reutilization. AcP showed a delayed single peak during late solventogenesis corresponding to acetate reutilization. As expected, in the pta mutant the AcP levels were very low, yet this strain exhibited strong butanol production. These data suggest that BuP is a regulatory molecule that may act as a phosphodonor of transcriptional factors. DNA array-based transcriptional analysis of the buk and M5 mutants demonstrated that high BuP levels corresponded to downregulation of flagellar genes and upregulation of solvent formation and stress genes. (+info)SpoIIE regulates sporulation but does not directly affect solventogenesis in Clostridium acetobutylicum ATCC 824. (2/98)
Using gene expression reporter vectors, we examined the activity of the spoIIE promoter in wild-type and spo0A-deleted strains of Clostridium acetobutylicum ATCC 824. In wild-type cells, the spoIIE promoter is active in a transient manner during late solventogenesis, but in strain SKO1, where the sporulation initiator spo0A is disrupted, no spoIIE promoter activity is detectable at any stage of growth. Strains 824(pMSpo) and 824(pASspo) were created to overexpress spoIIE and to decrease spoIIE expression via antisense RNA targeted against spoIIE, respectively. Some cultures of strains 824(pMSpo) degenerated during fermentations by losing the pSOL1 megaplasmid and hence did not produce the solvents ethanol, acetone, and butanol. The frequent degeneration event was shown to require an intact copy of spoIIE. Nondegenerate cultures of 824(pMSpo) exhibited normal growth and solvent production. Strain 824(pASspo) exhibited prolonged solventogenesis characterized by increased production of ethanol (225%), acetone (43%), and butanol (110%). Sporulation in strains harboring pASspo was significantly delayed, with sporulating cells exhibiting altered morphology. These results suggest that SpoIIE has no direct effect on the control of solventogenesis and that the changes in solvent production in spoIIE-downregulated cells are mediated by effects on the cell during sporulation. (+info)Heterologous production, assembly, and secretion of a minicellulosome by Clostridium acetobutylicum ATCC 824. (3/98)
The gene man5K encoding the mannanase Man5K from Clostridium cellulolyticum was cloned alone or as an operon with the gene cipC1 encoding a truncated scaffoldin (miniCipC1) of the same origin in the solventogenic Clostridium acetobutylicum. The expression of the heterologous gene(s) was under the control of a weakened thiolase promoter Pthl. The recombinant strains of the solventogenic bacterium were both found to secrete active Man5K in the range of milligrams per liter. In the case of the strain expressing only man5K, a large fraction of the recombinant enzyme was truncated and lost the N-terminal dockerin domain, but it remained active towards galactomannan. When man5K was coexpressed with cipC1 in C. acetobutylicum, the recombinant strain secreted almost exclusively full-length mannanase, which bound to the scaffoldin miniCipC1, thus showing that complexation to the scaffoldin stabilized the enzyme. The secreted heterologous complex was found to be functional: it binds to crystalline cellulose via the carbohydrate binding module of the miniscaffoldin, and the complexed mannanase is active towards galactomannan. Taken together, these data show that C. acetobutylicum is a suitable host for the production, assembly, and secretion of heterologous minicellulosomes. (+info)Expression of abrB310 and SinR, and effects of decreased abrB310 expression on the transition from acidogenesis to solventogenesis, in Clostridium acetobutylicum ATCC 824. (4/98)
The transcription factors sinR and abrB are involved in the control of sporulation initiation in Bacillus subtilis. We identified a single homologue to sinR and three highly similar homologues to abrB, designated abrB310, abrB1941, and abrB3647, in Clostridium acetobutylicum ATCC 824. Using reporter vectors, we showed that the promoters of abrB1941 and abrB3647 were not active under the growth conditions tested. The abrB310 promoter was strongly active throughout growth and exhibited a transient elevation of expression at the onset of solventogenesis. Primer extension assays showed that two transcripts of abrB310 and a single, extremely weak transcript for sinR are expressed. Potential -35 and -10 consensus motifs are readily identifiable surrounding the transcription start sites of abrB310 and sinR, with a single putative 0A box present within the promoter of abrB310. In strains of C. acetobutylicum transformed with plasmids to elevate sinR expression or decrease sinR expression, no significant differences in growth or in acid or solvent production were observed compared to the control strains. In C. acetobutylicum strain 824(pAS310), which expressed an antisense RNA construct targeted against abrB310, the acids acetate and butyrate accumulated to approximately twice the normal concentration. This accumulation corresponded to a delay and decrease in acetone and butanol production. It was also found that sporulation in strain 824(pAS310) was delayed but that the morphology of sporulating cells and spores was normal. Based upon these observations, we propose that abrB310 may act as a regulator at the transition between acidogenic and solventogenic growth. (+info)Homologous and heterologous overexpression in Clostridium acetobutylicum and characterization of purified clostridial and algal Fe-only hydrogenases with high specific activities. (5/98)
Clostridium acetobutylicum ATCC 824 was selected for the homologous overexpression of its Fe-only hydrogenase and for the heterologous expressions of the Chlamydomonas reinhardtii and Scenedesmus obliquus HydA1 Fe-only hydrogenases. The three Strep tag II-tagged Fe-only hydrogenases were isolated with high specific activities by two-step column chromatography. The purified algal hydrogenases evolve hydrogen with rates of around 700 micromol H(2) min(-1) mg(-1), while HydA from C. acetobutylicum (HydA(Ca)) shows the highest activity (5,522 micromol H(2) min(-1) mg(-1)) in the direction of hydrogen uptake. Further, kinetic parameters and substrate specificity were reported. An electron paramagnetic resonance (EPR) analysis of the thionin-oxidized HydA(Ca) protein indicates a characteristic rhombic EPR signal that is typical for the oxidized H cluster of Fe-only hydrogenases. (+info)Genomic analysis of the protein secretion systems in Clostridium acetobutylicum ATCC 824. (6/98)
Consistent information about protein secretion in Gram-positive bacteria is essentially restricted to the model organism Bacillus subtilis. Among genome-sequenced clostridia, Clostridium acetobutylicum has been the most extensively studied from a physiological point of view and is the organism for which the largest variety of molecular biology tools have been developed. Following in silico analyses, both secreted proteins and protein secretion systems were identified. The Tat (Twin arginine translocation; TC #2.A.64) pathway and ABC (ATP binding cassette) protein exporters (TC #3.A.1.) could not be identified, but the Sec (secretion) pathway (TC #3.A.5) appears to be used prevalently. Similarly, a flagella export apparatus (FEA; TC #3.A.6.), holins (TC #1.E.), and an ESAT-6/WXG100 (early secreted antigen target of 6 kDa/proteins with a WXG motif of approximately 100 residues) secretion system were identified. Here, we report for the first time the identification of a fimbrilin protein exporter (FPE; TC #3.A.14) and a Tad (tight adherence) export apparatus in C. acetobutylicum. This investigation highlights the potential use of this saprophytic bacterium in biotechnological and biomedical applications as well as a model organism for studying protein secretion in pathogenic Gram-positive bacteria. (+info)Diffusion, mixing, and associated dye effects in DNA-microarray hybridizations. (7/98)
Typical DNA microarrays utilize diffusion of dye-labeled cDNA probes followed by sequence-specific hybridization to immobilized targets. Here we experimentally estimated the distance typical probes travel during static 16-h hybridizations. Probes labeled with Cy3 and Cy5 were individually introduced to opposite sides of a microarray with minimal convective mixing. Oppositely labeled probes diffused across the initial front separating the two solutions, generating a zone with both dyes present. Diffusion-distance estimates for Cy3- and Cy5-labeled cDNAs were 3.8 mm and 2.6 mm, respectively, despite having almost identical molecular masses. In separate 16-h hybridization experiments with oppositely labeled probes premixed, arrays that were continuously mixed had 15-20% higher signal intensities than arrays hybridized statically. However, no change was observed in the Cy3/Cy5 signal intensity ratio between continuously mixed and static hybridizations. This suggests that the observed dye bias in diffusion-distance estimates results from differences in the detection limits of Cy3 and Cy5-labeled cDNA, a potential concern for array data on low-abundance transcripts. Our conservative diffusion-distance estimates indicate that replicate targets >7.6 mm apart will not compete for scarce probes. Also, raising the microarray gap height would delay the onset of diffusion-limited hybridization by increasing the amount of available probe. (+info)Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum. (8/98)
DNA microarray analysis of Clostridium acetobutylicum was used to examine the genomic-scale gene expression changes during the shift from exponential-phase growth and acidogenesis to stationary phase and solventogenesis. Self-organizing maps were used to identify novel expression patterns of functional gene classes, including aromatic and branched-chain amino acid synthesis, ribosomal proteins, cobalt and iron transporters, cobalamin biosynthesis, and lipid biosynthesis. The majority of pSOL1 megaplasmid genes (in addition to the solventogenic genes aad-ctfA-ctfB and adc) had increased expression at the onset of solventogenesis, suggesting that other megaplasmid genes may play a role in stationary-phase phenomena. Analysis of sporulation genes and comparison with published Bacillus subtilis results indicated conserved expression patterns of early sporulation genes, including spo0A, the sigF operon, and putative canonical genes of the sigma(H) and sigma(F) regulons. However, sigE expression could not be detected within 7.5 h of initial spo0A expression, consistent with the observed extended time between the appearance of clostridial forms and endospore formation. The results were compared with microarray comparisons of the wild-type strain and the nonsolventogenic, asporogenous M5 strain, which lacks the pSOL1 megaplasmid. While some results were similar, the expression of primary metabolism genes and heat shock proteins was higher in M5, suggesting a difference in metabolic regulation or a butyrate stress response in M5. The results of this microarray platform and analysis were further validated by comparing gene expression patterns to previously published Northern analyses, reporter assays, and two-dimensional protein electrophoresis data of metabolic genes (including all major solventogenesis genes), sporulation genes, heat shock proteins, and other solventogenesis-induced gene expression. (+info)
An improved kinetic model for the acetone-butanol-ethanol pathway of Clostridium acetobutylicum and model-based perturbation...
Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. -
Lancaster EPrints
Introducing a single secondary alcohol dehydrogenase into butanol-tolerant Clostridium acetobutylicum Rh8 switches ABE...
A two-plasmid inducible CRISPR/Cas9 genome editing tool for Clostridium acetobutylicum
Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag...
Clostridium acetobutylicum - Wikipedia
Clostridium acetobutylicum - Alchetron, the free social encyclopedia
Production of Acetone, Butanol, and Ethanol (ABE) by Clostridium acetobutylicum YM1 from Pretreated Palm Kernel Cake in Batch...
Characterization of iron reduction in Clostridium acetobutylicum mutants obtained by targeted and transposon mutagenesis
Clostridium acetobutylicum, Living, Tube | Carolina.com
TIGR01342
CEA/DSV/IBeB/SBVMe/LEMIRE - P2CS Database Clostridium acetobutylicum ATCC 824 plasmid pSOL1
clostridium acetobutylicum helps in the production of
INFLUENCE OF SULFATE AS AN ELECTRON ACCEPTOR ON THE ANAEROBIC HYDROLYSIS AND ACIDOGENESIS OF PARTICULATE ORGANICS
RCSB PDB - 3F10: Crystal structure of Clostridium Acetobutylicum 8-oxoguanine DNA glycosylase in complex with 8-oxoguanosine
Articles - LISBP Laboratoire dIngénierie des Systèmes Biologiques et des Procédés
Clostridium acetobutylicum McCoy et al. emend. Keis et al. | ATCC
lon - Lon protease - Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) - lon gene &...
RCSB PDB - 6AA8: Crystal structure of (S)-3-hydroxybutyryl-coenzymeA dehydrogenase from Clostridium acetobutylicum complexed...
CA C2547 - Possible membrane-associated methyl-accepting chemotaxis protein fused to HAMP domain - Clostridium acetobutylicum ...
Clostridium acetobutylicum | The Microbe Directory
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IGEM:UNAM/2009/Notebook/IGEM project/2011/04/27 - OpenWetWare
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Solvent Production Complex Unit
SOLVENT PRODUCTION AND DISTRIBUTION INDUSTRY - ALPHADYNAMIC PUMPS
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Effect of Butanol Challenge and Temperature on Lipid Composition and Membrane Fluidity of Butanol-Tolerant Clostridium...
Single-Amino Acid Modifications Reveal Additional Controls on the Proton Pathway of [FeFe]-Hydrogenase<...
Profile Facts: Alcohol fuel
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Army Research Laboratory initiative lends hand to economical waste-to-energy production | U.S. Army Research Laboratory
adsorption production biofilm acetobutylicum clostridium reactor biobutanol simultaneous integrated recovery figure
Spontaneous activation of [FeFe]-hydrogenases by an inorganic [2Fe] active site mimic
FFAS03 list of results C.acetobutylicum / all
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Liquid fuel
See also: Clostridium acetobutylicum. Butanol is an alcohol which can be used as a fuel in most gasoline internal combustion ... It is typically a product of the fermentation of biomass by the bacterium Clostridium acetobutylicum (also known as the ...
Butyrate kinase
Hartmanis MG (January 1987). "Butyrate kinase from Clostridium acetobutylicum". The Journal of Biological Chemistry. 262 (2): ... Wiesenborn DP, Rudolph FB, Papoutsakis ET (February 1989). "Phosphotransbutyrylase from Clostridium acetobutylicum ATCC 824 and ... "Sequence and arrangement of two genes of the butyrate-synthesis pathway of Clostridium acetobutylicum ATCC 824". Gene. 134 (1 ... "The central metabolic pathway from acetyl-CoA to butyryl-CoA in Clostridium acetobutylicum". FEMS Microbiology Reviews. 17 (3 ...
Glucan 1,4-a-glucosidase
Amylase French D, Knapp DW (December 1950). "The maltase of Clostridium acetobutylicum; its specificity range and mode of ...
Acetone-butanol-ethanol fermentation
It usually uses a strain of bacteria from the Class Clostridia (Family Clostridiaceae). Clostridium acetobutylicum is the most ... The ABE fermentation via Clostridium beijerinckii or Clostridium acetobutylicum for instance is characterized by product ... Although less effective, Clostridium beijerinckii and Clostridium saccharobutylicum bacterial strains have shown good results ... with Chaim Weizmann's isolation of Clostridium acetobutylicum, as described in U.S. patent 1315585. The Weizmann process was ...
Alcohol dehydrogenase
Walter KA, Bennett GN, Papoutsakis ET (November 1992). "Molecular characterization of two Clostridium acetobutylicum ATCC 824 ... Clostridium acetobutylicum NADPH- and NADH-dependent butanol dehydrogenases EC 1.1.1.- (genes adh1, bdhA and bdhB), enzymes ... Bacterial glycerol dehydrogenase EC 1.1.1.6 (gene gldA or dhaD). Clostridium kluyveri NAD-dependent 4-hydroxybutyrate ...
Chaim Weizmann
He used the bacterium Clostridium acetobutylicum (the Weizmann organism) to produce acetone. Acetone was used in the ...
Rubredoxin-NAD(+) reductase
"Isolation and properties of reduced nicotinamide adenine dinucleotiderubredoxin oxidoreductase of Clostridium acetobutylicum". ...
Richard W. Traxler
A coupled two-stage continuous fermentation for solvent production by Clostridium acetobutylicum. Enzyme and Microbial ...
List of sequenced bacterial genomes
2001). "Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum". J Bacteriol. ... 2003). "The genome sequence of Clostridium tetani, the causative agent of tetanus disease". Proc. Natl. Acad. Sci. U.S.A. 100 ( ... 2002). "Complete genome sequence of Clostridium perfringens, an anaerobic flesh-eater". Proc. Natl. Acad. Sci. U.S.A. 99 (2): ...
Butanol fuel
... process using Clostridium acetobutylicum, Clostridium beijerinckii. C. acetobutylicum was once used for the production of ... Anaerobic bacteria such as Clostridium acetobutylicum and Clostridium saccharobutylicum also contain these pathways. Succinate ... A strain of Clostridium can convert nearly any form of cellulose into butanol even in the presence of oxygen. A strain of ... The genes corresponding to these butanol production pathways from Clostridium were cloned to E. coli. Cyanobacteria are a ...
Butanol
Prior to the 1950s, Clostridium acetobutylicum was used in industrial fermentation to produce butanol. Research in the past few ...
Butanal dehydrogenase
"Purification and properties of the inducible coenzyme A-linked butyraldehyde dehydrogenase from Clostridium acetobutylicum". J ...
1-Butanol
Prior to the 1950s, Clostridium acetobutylicum was used in industrial fermentation to produce butanol. Research in the past few ... It is produced in small quantities in nearly all fermentations (see fusel oil). Clostridium produces much higher yields of ...
Immobilized enzyme
"Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production". ...
Cordite
He used the bacterium Clostridium acetobutylicum (the so-called Weizmann organism) to produce acetone. Weizmann transferred the ...
Glukan 1,4-a-glukozidaza - Википедија, слободна енциклопедија
French, D. & Knapp, D.W. (1950). „The maltase of Clostridium acetobutylicum". J. Biol. Chem. 187: 463-471. PMID 14803428.. ...
Metabolism
"Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol". Metabolic ...
Acetoacetate decarboxylase
... from Clostridium acetobutylicum catalyzes the decarboxylation of acetoacetate to yield acetone and ... In 1916, biochemist and future first president of Israel Chaim Weizmann was the first to isolate Clostridium acetobutylicum, a ... Acetoacetate decarboxylase has been found and studied in the following bacteria in addition to Clostridium acetobutylicum: ... In 2009, a crystal structure of acetoacetate decarboxylase from Clostridium acetobutylicum was solved, allowing Westheimer et ...
Two-component regulatory system
Treuner-Lange A, Kuhn A, Dürre P (Jul 1997). "The kdp system of Clostridium acetobutylicum: cloning, sequencing, and ... coli and Clostridium acetobutylicum. The N-terminal domain of this protein forms part of the cytoplasmic region of the protein ...
Riboswitch
... end processing A riboswitch in Clostridium acetobutylicum regulates an adjacent gene that is not part of the same mRNA ... "S-box and T-box riboswitches and antisense RNA control a sulfur metabolic operon of Clostridium acetobutylicum". Nucleic Acids ...
Acetic acid
... is produced and excreted by acetic acid bacteria, notably the genus Acetobacter and Clostridium acetobutylicum. ... However, Clostridium bacteria are less acid-tolerant than Acetobacter. Even the most acid-tolerant Clostridium strains can ... Species of anaerobic bacteria, including members of the genus Clostridium or Acetobacterium can convert sugars to acetic acid ... Sim, Jia Huey; Kamaruddin, Azlina Harun; Long, Wei Sing; Najafpour, Ghasem (2007). "Clostridium aceticum-A potential organism ...
Acetate kinase
"Cloning and sequence analysis of the genes encoding phosphotransbutyrylase and butyrate kinase from Clostridium acetobutylicum ...
Edward A. Bayer
... for biofuels production of a functional cell wall anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. ... 156, 828-36 (1983). Bayer, E. A., Kenig, R. & Lamed, R. Adherence of Clostridium thermocellum to cellulose. J. Bacteriol. 156, ... cellulase-containing complex in Clostridium thermocellum. J. Bacteriol. ...
Glycoside hydrolase family 25
Croux C, García JL (July 1991). "Sequence of the lyc gene encoding the autolytic lysozyme of Clostridium acetobutylicum ATCC824 ...
Methylglyoxal synthase
Huang K, Rudolph FB, Bennett GN (July 1999). "Characterization of methylglyoxal synthase from Clostridium acetobutylicum ATCC ...
Liquid fuel
It is typically a product of the fermentation of biomass by the bacterium Clostridium acetobutylicum (also known as the ...
Solar fuel
One example is the production of 1-butanol in Synechococcus elongatus using enzymes from Clostridium acetobutylicum, ...
Royal Naval Cordite Factory, Holton Heath
Chaim Weizmann had developed a process of bacterial fermentation, using Clostridium acetobutylicum, in 1912 but it did not ...
세포대사 - 위키백과, 우리 모두의 백과사전
"Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol". Metab Eng ...
Alcohol fuel
Clostridium acetobutylicum) currently used to perform these conversions produces an extremely unpleasant smell, and this must ...
Kategori:Rintisan bertopik bakteri bahasa Indonesia, ensiklopedia bebas
Clostridium acetobutylicum. *Clostridium botulinum. *Clostridium difficile. *Clostridium perfringens. *Corynebacterium ...
Biotechnology
... that of manufacturing corn starch using Clostridium acetobutylicum, to produce acetone, which the United Kingdom desperately ...
Kategori:Semua regnum bacteria bahasa Indonesia, ensiklopedia bebas
Clostridium acetobutylicum. *Clostridium butyricum. *Clostridium difficile. *Clostridium perfringens. *Corynebacterium ...
Bacterial phyla
... includes the genus Clostridium (e.g. the pathogens C. dificile, C. tetani, C. botulinum and the biotech C. acetobutylicum) ... Low-G+C species (later renamed Firmicutes[19]) (Clostridium, Peptococcus, Bacillus, Mycoplasma) ... the class Clostridia of mostly anaerobic sulphite-reducing saprophytic species, ...
Alcohol
The bacterium Clostridium acetobutylicum can feed on cellulose to produce butanol on an industrial scale.[28] ...
Buterna kiselina
Clostridium acetobutylicum, najistaknutiji producent acetona i propianola, koji se također koristi u industriji; ... Clostridium aurantibutyricum. Ove bakterije počinju sa fermentacijom butirata, kao što je opisano gore, ali, kada pH padne ...
Wikipedia:Wiki Sabanda II/daptar judul/C - Wikipédia Sunda, énsiklopédi bébas
Clostridium Acetobutylicum. *Clostridium Botulinum. *Clostridium Butyricum. *Clostridium Pasteurianum. *Clostridium Tetani. * ...
Clostridia
Clostridium botulinum (botulism). *Clostridium acetobutylicum. *Clostridium haemolyticum. *Clostridium novyi. *Clostridium ... The Clostridia are a highly polyphyletic class of Firmicutes, including Clostridium and other similar genera. They are ... Species of the class Clostridia are often but not always Gram-positive (see Halanaerobium hydrogenoformans) and have the ... Heliobacteria and Christensenella are also members of the class Clostridia. Some of the enzymes produced by this group are used ...
Clostridium acetobutylicum
EPA Clostridium acetobutylicum Final Risk Assessment. *Genetic Engineering of Clostridium acetobutylicum for Enhanced ... Clostridium acetobutylicum, ATCC 824, is a commercially valuable bacterium sometimes called the "Weizmann Organism", after ... Unlike yeast, which can digest only sugar into alcohol and carbon dioxide, C. acetobutylicum and other Clostridia can digest ... One of the crucial enzymes - a fatty acyl-CoA reductase - came from Clostridium acetobutylicum. ...
Baktérie - Wikipédia
Clostridium acetobutylicum), enzýmov, výroba liehu zo škrobu, mliekarenský priemysel, výroba vitamínov, antibiotik, alkaloidov ... Botulotoxín, produkt druhu Clostridium botulinum, je jedným z najsilnejších bakteriálnych toxínov vôbec. ...
Clostridium - Wikipedija, prosta enciklopedija
C. acetobutylicum. C. aerotolerans. C. botulinum. C. butyricum. C. colicanis. C. difficile. C. formicaceticum. C. ljungdahlii. ... Clostridium (klostridij) je rod grampozitivnih bakterij iz debla Firmicutes. Bakterije iz tega rodu so obvezni anaerobi in ... V rod Clostridium spadajo tako nenevarne vrste kot tudi pomembni patogeni. Štiri vrste povzročajo pri človeku bolezni: *C. ... Vzpostavljeno iz »https://sl.wikipedia.org/w/index.php?title=Clostridium&oldid=4843481« ...
Aesculus hippocastanum
The conkers were used as a source of starch for fermentation using the Clostridium acetobutylicum method devised by Chaim ...
Pathema
... -Clostridium Clostridium acetobutylicum Clostridium botulinum Clostridium butyricum Clostridium difficile Clostridium ... kluyveri Clostridium novyi Clostridium perfringens Clostridium phytofermentans Clostridium tetani Clostridium thermocellum ... Clostridium botulinum, Burkholderia mallei, Burkholderia pseudomallei, Clostridium perfringens, and Entamoeba histolytica) ...
PTS glucose-glucoside (Glc) family
For example, the solventogenic Clostridium acetobutylicum ATCC 824 has 13 altogether with 6 in the Glc family, 2 in the Fru ...
Clostridium acetobutylicum - Wikipedia
EPA Clostridium acetobutylicum Final Risk Assessment. *Genetic Engineering of Clostridium acetobutylicum for Enhanced ... Clostridium acetobutylicum, ATCC 824, is a commercially valuable bacterium sometimes called the "Weizmann Organism", after ... Unlike yeast, which can digest only sugar into alcohol and carbon dioxide, C. acetobutylicum and other Clostridia can digest ... One of the crucial enzymes - a fatty acyl-CoA reductase - came from Clostridium acetobutylicum. ...
Clostridium acetobutylicum, Living, Tube | Carolina.com
Clostridium acetobutylicum - microbewiki
Clostridium difficile, Clostridium botulinum, Clostridium tetani, and Clostridium perfringen. In particular, C. botulinum and C ... Clostridium acetobutylicum is a Gram-positive bacillus (1). C. acetobutylicum is most often soil dwelling, although it has been ... "Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium ... Adaptive responses to oxygen stress in obligatory anaerobes Clostridium acetobutylicum and Clostridium aminovalericum. Appl. ...
KEGG GENOME: Clostridium acetobutylicum DSM 1731
Integrated, systems metabolic picture of acetone-butanol-ethanol fermentation by Clostridium acetobutylicum | PNAS
2012) New insights into the butyric acid metabolism of Clostridium acetobutylicum. Appl Microbiol Biotechnol 96(5):1325-1339. ... 2004) Transcriptional analysis of butanol stress and tolerance in Clostridium acetobutylicum. J Bacteriol 186(7):2006-2018. ... 1994) Host-plasmid interactions in recombinant strains of Clostridium acetobutylicum ATCC 824. FEMS Microbiol Lett 123(3):335- ... 1984) The relationship between sporulation and solvent production in Clostridium acetobutylicum P262. Biotechnol Lett 6(8):529- ...
RCSB PDB - 2GLJ: crystal structure of aminopeptidase I from Clostridium acetobutylicum
Find proteins for Q97K30 (Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)) ... crystal structure of aminopeptidase I from Clostridium acetobutylicum. Min, T., Shapiro, L.. To be published. ... crystal structure of aminopeptidase I from Clostridium acetobutylicum. *DOI: 10.2210/pdb2GLJ/pdb ...
clostridium acetobutylicum butyric acid
Clostridium acetobutylicum, Clostridium saccharobutylicum and Clostridium beijerinckii are solventogenic Clostridia that grow ... New insights into the butyric acid metabolism of Clostridium acetobutylicum. Clostridium acetobutylicum is a Gram-positive ... By a butyric acid fermentation by Clostridium acetobutylicum resulted in clostridium acetobutylicum butyric acid number of ... Clostridia that grow by a butyric acid metabolism of Clostridium acetobutylicum has been considered an attractive platform-host ...
Clostridium acetobutylicum McCoy et al. emend. Keis et al. ATCC ®
Genomic DNA from Clostridium acetobutylicum TypeStrain=True Application: ... Clostridium acetobutylicum McCoy et al. emend. Keis et al. (ATCC® 824D-5™) Strain Designations: Genomic DNA from Clostridium ... Clostridium acetobutylicum McCoy et al. emend. Keis et al. ATCC® 824D-5™ dried At least 5 µg in 1X TE buffer. OD260/OD280: 1.6 ... Genomic DNA from Clostridium acetobutylicum [ATCC® 824™] Biosafety Level 1 Biosafety classification is based on U.S. Public ...
Clostridium acetobutylicum McCoy et al. emend. Keis et al. | ATCC
Clostridium acetobutylicum McCoy et al. emend. Keis et al. Depositors. NCTC Chain of custody. ATCC <-- NCTC <-- A.C. Thaysen ( ... The certificate of analysis for that lot of Clostridium acetobutylicum McCoy et al. emend. Keis et al. (4259) is not currently ... The certificate of origin for that lot of Clostridium acetobutylicum McCoy et al. emend. Keis et al. (4259) is not currently ... To download a certificate of origin for Clostridium acetobutylicum McCoy et al. emend. Keis et al. (4259), enter the lot number ...
KEGG PATHWAY: Streptomycin biosynthesis - Clostridium acetobutylicum ATCC 824
Improvement of butanol production in Clostridium acetobutylicum through enhancement of NAD(P)H availability | SpringerLink
Clostridium acetobutylicumis a natural producer of butanol, butyrate, acetone and ethanol. The pattern of metabolites reflects ... Clostridium acetobutylicum NAD(P)H Redox FdNR Ferredoxin TER Electronic supplementary material. The online version of this ... Clostridium acetobutylicum is a natural producer of butanol, butyrate, acetone and ethanol. The pattern of metabolites reflects ... Cooksley CM, Zhang Y, Wang H, Redl S, Winzer K, Minton NP (2012) Targeted mutagenesis of the Clostridium acetobutylicum acetone ...
Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum. | Journal of Bacteriology
Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum.. U Sauer, A Treuner, M Buchholz, J D ... Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum.. U Sauer, A Treuner, M Buchholz, J D ... Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum.. U Sauer, A Treuner, M Buchholz, J D ... Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum. Message Subject (Your Name) has forwarded ...
Molecular modulation of pleiotropic regulator CcpA for glucose and xylose coutilization by solvent-producing Clostridium...
... solvents production from lignocellulosic hydrolysates by Clostridium acetobutylicum, an important industrial microorganism. ... modulation of pleiotropic regulator CcpA for glucose and xylose coutilization by solvent-producing Clostridium acetobutylicum. ... acetobutylicum. When this residue was replaced by asparagine (V302N mutation), CCR could be alleviated and a greatly improved ... acetobutylicum CcpA with alleviated repression on xylose metabolism, yielding a valuable platform host toward ABE solvents ...
Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824. -...
The effect of CO on growth and product formation in batch cultures of Clostridium acetobutylicum | SpringerLink
... acetobutylicum inhibits the production of H2by the hydrogenase and enhances the production of solvents by making available ... The effect of CO on growth and product formation in batch cultures ofClostridium acetobutylicum. ... Carbon monoxide sparged in batch fermentations ofC. acetobutylicum inhibits the production of H2 by the hydrogenase and ...
A Synthetic Biology Approach to Enhancing Metabolic Engineering of Clostridium Acetobutylicum | SBE | Society for Biological...
n-Butanol is a natural product of the fermentation of carbohydrates by Clostridium acetobutylicum and can be used as biofuel ... A Synthetic Biology Approach to Enhancing Metabolic Engineering of Clostridium Acetobutylicum. *Conference: Metabolic ... Formerly, C. acetobutylicum was used in the industrial Acetone-Butanol-Ethanol (ABE) fermentation to produce solvents in a ... Heap, J.T., Pennington, O.J., Cartman, S.T. and Minton, N.P. (2009) A modular system for Clostridium shuttle plasmids. J. ...
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)
Clostridium acetobutylicum DSM 792. › Clostridium acetobutylicum str. ATCC 824. › Clostridium acetobutylicum strain ATCC 824. ... Clostridia. › Clostridiales. › Clostridiaceae. › Clostridium. › Clostridium acetobutylicum. Strains i. › ATCC 824 / DSM 792 / ... Taxonomy - Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) Basket 0 ... Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787). ...
Molecular and genomic analyses in Clostridium acetobutylicum
This dissertation presents molecular and genomic analyses in Clostridium acetobutylicum, beginning with an evaluation of Spo0A ... These proteins are novel and essentially limited to C. acetobutylicum; thus, suggesting roles specific to its unique physiology ... "Molecular and genomic analyses in Clostridium acetobutylicum." (2009) Diss., Rice University. https://hdl.handle.net/1911/61756 ...
RCSB PDB - 6AA8: Crystal structure of (S)-3-hydroxybutyryl-coenzymeA dehydrogenase from Clostridium acetobutylicum complexed...
Clostridium acetobutylicum ATCC 824. Mutation(s): 0 Gene Names: hbd, CA_C2708. EC: 1.1.1.157. ... Find proteins for P52041 (Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)) ... Crystal structure of (S)-3-hydroxybutyryl-coenzymeA dehydrogenase from Clostridium acetobutylicum complexed with NAD+. *DOI: ... 3-hydroxybutyryl-CoA dehydrogenase from Clostridium acetobutylicum.. Takenoya, M., Taguchi, S., Yajima, S.. (2018) Acta ...
Cloning and sequencing of genes involved in glycolysis from Clostridium acetobutylicum
Complementation studies were carried out with a Clostridium acetobutylicum ATCC 824 plasmid library and Escherichia coli ... "Cloning and sequencing of genes involved in glycolysis from Clostridium acetobutylicum." (1996) Masters Thesis, Rice ... Phosphofructokinase from C. acetobutylicum has high homology to the phosphofructokinases from Bacillus stearothermophilus, ... The pyruvate kinase/phosphofructokinase operon may act as a "regulatory operon" for glycolysis in C. acetobutylicum. ...
Characterization of the CipA Scaffolding Protein and In Vivo Production of a Minicellulosome in Clostridium acetobutylicum |...
Characterization of the CipA Scaffolding Protein and In Vivo Production of a Minicellulosome in Clostridium acetobutylicum. ... Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J. Bacteriol. 183:4823- ... The cipA gene encoding the Clostridium acetobutylicum scaffolding protein CipA was cloned and expressed in Escherichia coli. ... Clostridium acetobutylicum is a gram-positive, sporeforming anaerobic bacterium that converts various sugars and ...
lon - Lon protease - Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) - lon gene &...
sp,Q97FT9,LON_CLOAB Lon protease OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) ... Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787). ... Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) ... cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › ...
Send to a Friend: Production of Isopropanol Butanol Ethanol Mixture by Engineered <I>Clostridium acetobutylicum</I> - Biofuels...
Characterization of iron reduction in Clostridium acetobutylicum mutants obtained by targeted and transposon mutagenesis
Adaptive Responses to Oxygen Stress in Obligatory Anaerobes Clostridium acetobutylicum and Clostridium aminovalericum | Applied...
Clostridium acetobutylicum and Clostridium aminovalericum, both typical Clostridium species, grow normally under continuous ... Adaptive Responses to Oxygen Stress in Obligatory Anaerobes Clostridium acetobutylicum and Clostridium aminovalericum. Shinji ... Clostridium acetobutylicum and Clostridium aminovalericum, both obligatory anaerobes, grow normally after growth conditions are ... Bacterial strains and media. Clostridium aminovalericum NRIC0223 (= DSM1283) and Clostridium acetobutylicum DSM792 (= ATCC 824 ...
A Novel Dual-cre Motif Enables Two-Way Autoregulation of CcpA in Clostridium acetobutylicum | Applied and Environmental...
... cre motif that is employed by the CcpA in Clostridium acetobutylicum, a typical solventogenic Clostridium species, for ... we identified the CcpA protein in Clostridium acetobutylicum, a typical solventogenic Clostridium species that has been widely ... A Novel Dual-cre Motif Enables Two-Way Autoregulation of CcpA in Clostridium acetobutylicum. Lu Zhang, Yanqiang Liu, Yunpeng ... Thiolase from Clostridium acetobutylicum ATCC 824 and its role in the synthesis of acids and solvents. Appl Environ Microbiol ...
Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. -
Lancaster EPrints
Thiolase of Clostridium acetobutylicum is an important enzyme involved in both, acid and solvent fermentation. Two thiolase ... have been cloned and sequenced from Clostridium acetobutylicum DSM 792, showing high homology to each other and to thiolases of ... Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. Journal of Molecular Microbiology and ...
Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. - Semantic Scholar
As C. acetobutylicum is unable to grow on cellulosic substrates, the existence of a cellulosomal gene cluster in the genome ... Surprisingly, genetic organization of this large cluster is very similar to that of Clostridium cellulolyticum, the model of ... A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. ... A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. ...
DNA topology and gene expression in Clostridium acetobutylicum: Implications for the regulation of solventogenesis - Semantic...
... and DNA topology in Clostridium acetobutylicum was investigated by inhibiting DNA gyrase in vivo by high concentrations of ... Transcriptional regulation of solventogenesis in Clostridium acetobutylicum.. *Peter Dürre, Michael Böhringer, Stephan Nakotte ... Cloning and expression of Clostridium acetobuty - licum genes involved in solvent production. *M. Sebald, P. Diirre, +7 authors ... DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5.. * ...
ATCCStrainsEthanolBacteriaGenusBeijerinckii NCIMB 8052SpeciesStrainBacteriumAbstractUtilizationThermocellumEscherichiaOrganismButyricumCells of Clostridium acetobutylicumGenes in Clostridium acetobutylicumGene from Clostridium acetobutylicumCultures of Clostridium acetobutylicumGenome sequenceDehydrogenaseIsolated by colony hybMetabolicOperonBiofuelsPATHWAYSolventEnzymeP262AnaerobeCharacterizationHydrogenase protein productXyloseSolventogenesisProductionPasteurianumNCBIProteinsMetabolismBacillusRedox balanceMolecular
ATCC22
- Clostridium acetobutylicum , ATCC 824, is a commercially valuable bacterium sometimes called the " Weizmann Organism ", after Jewish-Russian-born Chaim Weizmann . (wikipedia.org)
- ATCC reference organism 824 C.Acetobutylicum. (wikipedia.org)
- Clostridium acetobutylicum ATCC 824 is considered the type strain. (kenyon.edu)
- The genome of Clostridium acetobutylicum ATCC 824 has been sequenced using the shotgun approach. (kenyon.edu)
- Other, less studied strains of C. acetobutylicum such as ATCC 4259 have shown similar degeneration. (kenyon.edu)
- The integrated model was able to successfully reproduce ABE fermentations of the WT C. acetobutylicum (ATCC 824), as well as its mutants, using data obtained from our own experiments and from literature. (pnas.org)
- Clostridium acetobutylicum ATCC 824, complete genome. (atcc.org)
- Clark SW, Bennett GN, Rudolph FB (1989) Isolation and characterization of mutants of Clostridium acetobutylicum ATCC 824 deficient in acetoacetyl-coenzyme A:acetate/butyrate: coenzyme A-transferase (EC 2.8.3.9) and in other solvent pathway enzymes. (springer.com)
- Green EM, Boynton ZL, Harris LM, Rudolph FB, Papoutsakis ET, Bennett GN (1996) Genetic manipulation of acid formation pathways by gene inactivation in Clostridium acetobutylicum ATCC 824. (springer.com)
- Complementation studies were carried out with a Clostridium acetobutylicum ATCC 824 plasmid library and Escherichia coli glycolytic mutants. (rice.edu)
- A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. (semanticscholar.org)
- The issue of secretion in heterologous expression of Clostridium cellulolyticum cellulase-encoding genes in Clostridium acetobutylicum ATCC 824. (semanticscholar.org)
- After sequencing, C. acetobutylicum ATCC 824, a series of genes very similar to the nitrogen fixing genes in C. pasteurianum were found, further confirming the bacterium's ability to utilize atmospheric nitrogen (8). (jakr-brno.cz)
- It could be something as simple as a run The genes for butanol and acetone formation in Clostridium acetobutylicum ATCC 824 reside on a large plasmid whose loss leads to degeneration of the strain. (jakr-brno.cz)
- The resulting contigs were reordered using SOAPdenovo 1.3 and C. acetobutylicum ATCC 824 as a reference genome (GenBank accession number NC_003030.1). (asm.org)
- The orthologous genes between C. acetobutylicum GXAS18-1 and C. acetobutylicum ATCC 824 were identified using SEED functional comparison ( 10 ). (asm.org)
- The C. acetobutylicum GXAS18-1 chromosome exhibits a high level of synteny to C. acetobutylicum ATCC 824, with the exception of 104 unique genes in the former chromosome and 64 genes in the latter, respectively. (asm.org)
- Simultaneous production of isopropanol, butanol, ethanol and 2,3-butanediol by Clostridium acetobutylicum ATCC 824 engineered strains. (archives-ouvertes.fr)
- In order to increase isopropanol production, the gene encoding the secondary-alcohol dehydrogenase enzyme from C. beijerinckii NRRL B593 (adh) which catalyzes the reduction of acetone to isopropanol, was cloned into the acetone, butanol and ethanol (ABE)-producing strain C. acetobutylicum ATCC 824. (archives-ouvertes.fr)
- Characterisation of a glucose phosphotransferase system in Clostridium acetobutylicum ATCC 824. (napier.ac.uk)
- Characterisation of class II and III fructose bisphosphatases in Clostridium acetobutylicum ATCC 824. (napier.ac.uk)
- These putative clusters included some putative metabolic operons comparable to those found in other reference strains such as C. beijerinckii NCIMB 8052 or C. acetobutylicum ATCC 824. (deepdyve.com)
Strains12
- Here the exogenous genes of ferredoxin-NAD(P) + oxidoreductase (FdNR) and trans -enoyl-coenzyme reductase (TER) are introduced to three different Clostridium acetobutylicum strains to investigate the distribution of redox equivalents and butanol productivity. (springer.com)
- While the TER functions as an NAD(P)H oxidase, butanol production was decreased in the C. acetobutylicum strains containing TER. (springer.com)
- This strategy will also be employed in the future to create high-yield strains of C. acetobutylicum and other industrially relevant strains for production of a wide range of commodity chemicals. (aiche.org)
- DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5. (semanticscholar.org)
- Various methods of culture maintenance and inoculum develop-ment were evaluated for their effectiveness in conserving and improving the property of 2 strains of Clostridium acetobutyicum, namely NCIB 2951 and NRRL B-594, to produce solvents by fermentation of whey permeate. (massey.ac.nz)
- Clostridium acetobutylicum is one group of Clostridium strains that is generally used to produce acetone and butanol through traditional acetone-butanol-ethanol fermentation (ABE) ( 1 ). (asm.org)
- However, the industrial application of Clostridium acetobutylicum strains for high-performance solvent yields and their extreme tolerance for butanol still require more investigation. (asm.org)
- In nature, isopropanol is excreted by some strains of Clostridium beijerinckii, simultaneously with butanol and ethanol during the isopropanol butanol ethanol (IBE) fermentation. (archives-ouvertes.fr)
- The goal of this study was to obtain mutant strains of Clostridium beijerinckii NRRL B-598 with improved butanol tolerance using random chemical mutagenesis, describe changes in their phenotypes compared to the wild-type strain and reveal changes in the genome that explain improved tolerance or other phenotypic changes. (frontiersin.org)
- The fermentation process by Clostridium strains has been known to be the most efficient one for n-butanol production. (eurekalert.org)
- From 1912 to 1914 strains of Clostridium acetobutylicum were isolated by Chaim Weizmann which had the ability of fermenting starchy substrate. (appropedia.org)
- The two-stage, dual-path process, which relies on two different Clostridia strains ( earlier post ), also yields hydrogen as a product. (greencarcongress.com)
Ethanol21
- ABE Acetone Butanol Clostridium beijerinckii Ethanol Nölling J, Breton G, Omelchenko MV, et al. (wikipedia.org)
- One canonical example of such processes is acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum , during which cells convert carbon sources to organic acids that are later reassimilated to produce solvents as a strategy for cellular survival. (pnas.org)
- genic species is Clostridium acetobutylicum, which typically pro-duces butanol, acetone, and ethanol at the mass ratio of 6:3:1 (17, 20, 21). (jakesonline.org)
- Abstract Biosynthesis of acetone and n-butanol is naturally restricted to the group of solventogenic clostridia with Clostridium acetobutylicum being the model organism for acetone-butanol-ethanol (ABE) fermentation. (jakesonline.org)
- Clostridium acetobutylicum is a natural producer of butanol, butyrate, acetone and ethanol. (springer.com)
- Biswas R, Zheng T, Olson DG, Lynd LR, Guss AM (2015) Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum . (springer.com)
- Cooksley CM, Zhang Y, Wang H, Redl S, Winzer K, Minton NP (2012) Targeted mutagenesis of the Clostridium acetobutylicum acetone-butanol-ethanol fermentation pathway. (springer.com)
- Efficient cofermentation of hexose and pentose sugars is essential for ABE (Acetone, Butanol and Ethanol) solvents production from lignocellulosic hydrolysates by Clostridium acetobutylicum, an important industrial microorganism. (nih.gov)
- Formerly, C. acetobutylicum was used in the industrial Acetone-Butanol-Ethanol (ABE) fermentation to produce solvents in a ratio of 3 parts acetone, 6 parts butanol to 1 part ethanol. (aiche.org)
- Cellulase, clostridia, and ethanol. (semanticscholar.org)
- C. acetobutylicum was the most reported in acetone-butanol-ethanol (ABE) fermentation for synthesis of biobutanol with higher yields [5, 6]. (alertamigratoria.org)
- Clostridium pasteurianum has a non-biphasic metabolism, alternatively producing 1,3-propanediol (PDO)-butanol-ethanol, referred to as PBE fermentation. (casablanca-kamyk.pl)
- The regA gene inhibited the expression of a C. acetobutylicum gene that allows the starch to degrade into acetone, butanol and ethanol. (readabstracts.com)
- This study investigates the production of acetone, butanol and ethanol (ABE) in sterilized sludge of palm oil mill effluent (POME) with the addition of glucose by Clostridium acetobutylicum NCIMB 619. (elsevier.com)
- Acetone, butanol, and ethanol (ABE) were produced from corn fiber arabinoxylan (CFAX) and CFAX sugars (glucose, xylose, galactose, and arabinose) using Clostridium acetobutylicum P260. (ingentaconnect.com)
- Clostridium acetobutylicum is an organism involved in the production of acetone and butanol by traditional acetone-butanol-ethanol fermentation (ABE). (asm.org)
- Clostridia, well-known commercial biobutanol production organisms, have the capability to convert a wide variety of renewable biomass and agricultural waste materials to butanol and ethanol. (asm.org)
- Artificial electron carriers have been widely used to shift the solvent ratio towards butanol in acetone-butanol-ethanol (ABE) fermentation of solventogenic clostridia according to decreased hydrogen production. (omicsdi.org)
- Palm kernel cake (PKC) is an abundant biomass generated from the palm oil processing industry that can be used as the carbon source for the growth and production of acetone-butanol-ethanol fermentation (ABE) by Clostridia. (ncsu.edu)
- abstract = "Present investigation explores the use of pineapple peel, a food industry waste, for acetone-butanol- ethanol (ABE) production using Clostridium acetobutylicum B 527. (aalto.fi)
- Improved operating strategy for continuous fermentation of carbon monoxide to fuel-ethanol by clostridia. (uni-ulm.de)
Bacteria9
- findarticles.com: Bacteria speeds drug to tumors - use of Clostridium acetobutylicum enzyme to activate cancer drug CB 1954. (wikipedia.org)
- In addition to being an important bacteria for industrial use, C. acetobutylicum is studied as model for endospore formation in bacteria. (kenyon.edu)
- Understanding the pathways of endospore formation is important because many endospore forming bacteria are human pathogens, in both the Bacillus and Clostridium genera. (kenyon.edu)
- Bacteria belonging to the genus Clostridium are classified as obligatory anaerobes ( 26 , 62 ) and are widely used in the field of solvent fermentation, biodegradation, and microbial energy production. (asm.org)
- Two thiolase genes (thlA and thlB) have been cloned and sequenced from Clostridium acetobutylicum DSM 792, showing high homology to each other and to thiolases of PHA-synthesizing bacteria. (lancs.ac.uk)
- The anaerobe Clostridium acetobutylicum belongs to the most important industrially used bacteria. (leibniz-hki.de)
- Daegu and Daejeon, Republic of Korea, September 22, 2015--Two Korean research teams at the Kyungpook National University (KNU) and the Korea Advanced Institute of Science and Technology (KAIST) have succeeded in uncovering the redox-switch of thiolase, a key enzyme for n-butanol production in Clostridium acetobutylicum , one of the best known butanol-producing bacteria. (eurekalert.org)
- When biologists seed a fresh batch of sugary broth with C. acetobutylicum bacteria, the first thing those microbes do is harvest the sugar's energy and multiply-their simple method of reproduction. (icr.org)
- The left side of this image depicts cell fusion between Clostridium ljungdahlii and C. acetobutylicum bacteria as seen through fluorescence microscopy. (genengnews.com)
Genus3
- Clostridium butyricum , a type species of the genus Clostridium , has the ability to resume growth after oxygen consumption without any lasting damage ( 33 ). (asm.org)
- On the basis of these inconclusive criteria, species of the genus Clostridium reflect a large heterogeneous group with pheno- and genotypical diversity. (alertamigratoria.org)
- Clostridium beijerinckii is a gram positive, rod shaped, motile bacterium of the genus Clostridium. (wikipedia.org)
Beijerinckii NCIMB 80521
- Evidence for the presence of an alternative glucose transport system in Clostridium beijerinckii NCIMB 8052 and the solvent hyper-producing mutant BA101. (napier.ac.uk)
Species5
- Biofuel Research Journal 6 … produce butyric acid and acetic acid via the butyric acid fermentation pathway.Some species of Clostridium, such as C. acetobutylicum also produce isopropanol or acetone. (jakesonline.org)
- In C. acetobutylicum , genes suggested to be involved in oxygen metabolism and genes for reactive oxygen species (ROS) scavenging were chosen from the genome database. (asm.org)
- Here, we report a novel dual- cre motif that is employed by the CcpA in Clostridium acetobutylicum , a typical solventogenic Clostridium species, for autoregulation. (asm.org)
- The most famous species of the group of solventogenic clostridia are Clostridium acetobutylicum and Clostridium beijerinckii , both of which share a common process bottleneck - low tolerance to butanol. (frontiersin.org)
- Fluorescence microscopy and flow cytometry of one species with stained RNA and the other tagged with a fluorescent protein demonstrated extensive RNA exchange and identified hybrid cells, some of which continued to divide, while some were in an advanced C. acetobutylicum sporulation form. (genengnews.com)
Strain9
- Pathema-Clostridium Resource Chaim Weizmann Type strain of Clostridium acetobutylicum at BacDive - the Bacterial Diversity Metadatabase M. Goho, Alexandra (2008-01-16). (wikipedia.org)
- When Clostridium acetobutylicum is cultured in continuous culture or undergoes many transfers, the strain slowly degenerates in that it loses its ability for solvent production. (kenyon.edu)
- Complete genome sequence of Clostridium acetobutylicum DSM 1731, a solvent-producing strain with multireplicon genome architecture. (genome.jp)
- A butanol production of 9.01 g/L (36.9% higher than the control), and the highest ratios of butanol/acetate (7.02) and C 4 /C 2 (3.17) derived metabolites were obtained in the C acetobutylicum buk - strain expressing FdNR. (springer.com)
- Subsequently, we attempted to create a high-yield strain of C. acetobutylicum by employing synthetic regulatory elements to develop and implement a system for metabolic flux redistribution. (aiche.org)
- This study provides new insights into the regulatory role of CcpA in C. acetobutylicum and, moreover, contributes a new engineering strategy for this industrial strain. (asm.org)
- While the type strain of C. acetobutylicum was isolated from soil, C. acetobutylicum is ubiquitous. (jakr-brno.cz)
- To reinforce a metabolic flux toward butanol production, the metabolic network of C. acetobutylicum strain was engineered with the redesigned enzyme. (eurekalert.org)
- Conclusions: We present the full genome sequence of Clostridium beijerinckii DSM 6423, providing a complete genetic background of this strain. (deepdyve.com)
Bacterium6
- A typical ABE fermentation using C. acetobutylicum results in a molar yield of 3:6:1 for acetone, butanol, and 2 BUTANOL PRODUCTION 5 Figure 2: Biochemical pathways in C. acetobutylicum Considering its capability to overproduce butanol through butyrate, it was thought that butyric acid can also be efficiently produced by this bacterium through metabolic engineering. (jakesonline.org)
- Clostridium acetobutylicum is a gram-positive, sporeforming anaerobic bacterium that converts various sugars and polysaccharides into acids and solvents ( 7 ). (asm.org)
- Clostridium acetobutylicum is an appropriate Gram-positive bacterium to study those mechanisms as genetic tools have been developed due to its industrial interest, allowing easy targeted and random mutagenesis. (epfl.ch)
- reported that Clostridium glycolicum , an acetogenic bacterium, can grow in the presence of oxygen (under up to 6% headspace oxygen in static culture) with oxygen-consuming activities. (asm.org)
- In this study the transcriptional behavior of the natural solvent producing bacterium Clostridium acetobutylicum was investigated following n-butanol stress using DNA microarray analysis. (omicsdi.org)
- Clostridium acetobutylicum is a Gram-positive, endospore-forming bacterium that is considered as a strict anaerobe. (omicsdi.org)
Abstract1
- abstract = "Summary The phosphorylated Spo0A transcription factor controls the initiation of endospore formation in Clostridium acetobutylicum, but genes encoding key phosphorelay components, Spo0F and Spo0B, are missing in the genome. (aber.ac.uk)
Utilization5
- The recent fossil fuel crisis has spurred more research into C. acetobutylicum and the utilization of the ABE process (2) . (kenyon.edu)
- They were then successfully exploited … and butanol by C. acetobutylicum in the utilization of agricultural and domestic wastes such as whey, wood shavings, bagasse, and rice straw has also been investigated (McNeil and Kristiansen, 1986). (jakesonline.org)
- We then present experimental evidence that the cel operon involved in cellobiose utilization is directly regulated by CelR and σ 54 (SigL) in Clostridium acetobutylicum. (elsevier.com)
- enhance the utilization ability of C. acetobutylicum on glucose and direct most of extra consumed glucose into acetone/butanol synthesis routes. (usda.gov)
- It is the first utilization of this approach for the development of butanol-tolerant mutants of solventogenic clostridia, for which generally there is a lack of knowledge about butanol efflux or efflux mechanisms and their regulation. (frontiersin.org)
Thermocellum4
- CBD3a displays 46% and 42% identity with the cellulose-binding domains from Clostridium thermocellum and Clostridium cellulolyticum , respectively. (asm.org)
- LacI Transcriptional Regulatory Networks in Clostridium thermocellum DSM1313. (semanticscholar.org)
- Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL-protein reveals an unusual degree of internal homology. (semanticscholar.org)
- Identification of the cellulose-binding domain of the cellulosome subunit S1 from Clostridium thermocellum YS. (semanticscholar.org)
Escherichia4
- Bannam TL, Rood JI (1993) Clostridium perfringens- Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. (springer.com)
- Furthermore, to investigate the catalytic residues of CacHBD, the enzymatic activities of the wild type and of three single-amino-acid mutants were analyzed, in which the Ser, His and Asn residues that are conserved in the HBDs from C. acetobutylicum, C. butyricum and Ralstonia eutropha, as well as in the L-3-hydroxyacyl-CoA dehydrogenases from Homo sapiens and Escherichia coli, were substituted by alanines. (rcsb.org)
- Phosphofructokinase from C. acetobutylicum has high homology to the phosphofructokinases from Bacillus stearothermophilus, Bacillus macquarensis, Escherichia coli and other microbes. (rice.edu)
- The cipA gene encoding the Clostridium acetobutylicum scaffolding protein CipA was cloned and expressed in Escherichia coli . (asm.org)
Organism1
- Thus, it is fitting that C. acetobutylicum is often called the "Weizmann organism. (kenyon.edu)
Butyricum3
- Comparison of toxins of Clostridium butyricum and Clostridium botulinum type E. Infection and Immunity 56:926-929. (jakr-brno.cz)
- Clostridium butyricum is to our knowledge the best natural 1,3-propanediol producer from glycerol and the only microorganism identified so far to use a coenzyme B12-independent glycerol dehydratase. (ucp.pt)
- The 1,3- propanediol pathway from C. butyricum was introduced on a plasmid in several mutants of C. acetobutylicum altered in product formation. (ucp.pt)
Cells of Clostridium acetobutylicum1
- A stable solvent productivity of 3.5 g/L h was achieved by using cells of Clostridium acetobutylicum immobilized onto a packed bed of bonechar, coupled with continuous product removal by pervaporation. (membran.at)
Genes in Clostridium acetobutylicum1
- Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum. (asm.org)
Gene from Clostridium acetobutylicum1
- Characterization and expression of the hydrogenase-encoding gene from Clostridium acetobutylicum P262. (readabstracts.com)
Cultures of Clostridium acetobutylicum1
- In this study, first insights on the molecular level were gained to explore the effect of methyl viologen addition to cultures of Clostridium acetobutylicum. (omicsdi.org)
Genome sequence3
- Here, we briefly describe the draft genome sequence of C. acetobutylicum GXAS18-1. (asm.org)
- Complete genome sequence of the autotrophic acetogen Clostridium formicaceticum DSM 92 using nanopore and llumina sequencing data. (uni-ulm.de)
- Genome sequence of the strict anaerobe Clostridium neopropionicum X4, DSM 3847T. (uni-ulm.de)
Dehydrogenase3
- The enzyme 3-hydroxybutyryl coenzyme A dehydrogenase, a key enzyme in solventogenesis in Clostridium acetobutylicum , was among the most up-regulated proteins during high toxin production. (jakesonline.org)
- The purification and isolation of glyceraldehyde-3-phosphate dehydrogenase from Clostridium acetobutylicum was accomplished by sequential ammonium sulfate precipitation, gel filtration and anion-exchange chromatography. (readabstracts.com)
- An NADP-dependent alcohol dehydrogenase from Clostridium acetobutylicum (CaADH) has been expressed and characterized. (nebraska.edu)
Isolated by colony hyb2
- DNA sequencing of the hydrogenase-encoding gene (hydA) isolated by colony hybridization from Clostridium acetobutylicum P262 reveals the presence of an open reading frame of 1722 bp that codes for a protein with 574 amino acids. (readabstracts.com)
- This gene was isolated by colony hybridization, using the Clostridium pasteurianum hydrogenase-1 gene as a probe. (microbiologyresearch.org)
Metabolic7
- Clostridium acetobutylicum has been considered an attractive platform-host for biorefinery due to its metabolic diversity. (jakesonline.org)
- Bennett GN, Rudolph FB (1995) The central metabolic pathway from acetyl-CoA to butyryl-CoA in Clostridium acetobutylicum . (springer.com)
- Oxygen has a crucial effect on the growth of clostridia, but the mechanisms of growth inhibition, as well as the existence of O 2 metabolic systems, remain unknown. (asm.org)
- In the paper published in Nature Communications, the researchers show that the redox-switch of thiolase plays a role in a regulation of metabolic flux in C. acetobutylicum by using in silico modeling and simulation tools. (internationalsugarjournal.com)
- To obtain a better ''vitamin B12-free'' biological process, we developed a metabolic engineering strategy with Clostridium acetobutylicum. (ucp.pt)
- Interestingly, all transformants with the adh gene converted acetoin (a minor fermentation product) into 2,3-butanediol, highlighting the wide metabolic versatility of solvent-producing Clostridia. (archives-ouvertes.fr)
- The combination of the discovery of 3D enzyme structure and systems metabolic engineering approaches resulted in increased n-butanol production in C. acetobutylicum , which allows the production of this important industrial chemical to be cost competitive. (eurekalert.org)
Operon4
- The pyruvate kinase/phosphofructokinase operon may act as a "regulatory operon" for glycolysis in C. acetobutylicum. (rice.edu)
- Molecular analysis of the mannitol operon of Clostridium acetobutylicum encoding a phosphotransferase system and a putative PTS-modulated regulator. (microbiologyresearch.org)
- Northern blot analysis reveals the translation of the C. acetobutylicum hydrogenase protein product to be from a monocistronic operon. (readabstracts.com)
- Northern blot analysis of RNA isolated from C. acetobutylicum indicates that the C. acetobutylicum hydrogenase protein product is translated from a monocistronic operon. (microbiologyresearch.org)
Biofuels1
- Clostridium acetobutylicum has received renewed interest worldwide as a promising producer of biofuels and bulk chemicals such as n-butanol, 1,3-propanediol, 1,3-butanediol, isopropanol, and butyrate. (casablanca-kamyk.pl)
PATHWAY3
- Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824. (nih.gov)
- Additionally, updates to the C. acetobutylicum genome annotation are proposed, including the identification of genes likely to encode proteins involved in the metabolism of arabinose and xylose via the pentose phosphate pathway. (microbiologyresearch.org)
- The gap gene, which was cloned and sequenced from C. acetobutylicum DSM 792, was observed to be clustering with other genes of enzymes from the glycolytic pathway. (readabstracts.com)
Solvent6
- Solvent production by Clostridium acetobutylicum collapses when cells are grown in pH-uncontrolled glucose medium, the so-called "acid crash" phenomenon. (jakesonline.org)
- Molecular modulation of pleiotropic regulator CcpA for glucose and xylose coutilization by solvent-producing Clostridium acetobutylicum. (nih.gov)
- This dissertation presents molecular and genomic analyses in Clostridium acetobutylicum, beginning with an evaluation of Spo0A, a transcriptional regulator required for activation of solvent production genes. (rice.edu)
- Thiolase of Clostridium acetobutylicum is an important enzyme involved in both, acid and solvent fermentation. (lancs.ac.uk)
- Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and changes in the cell's transcriptional program. (semanticscholar.org)
- 9) Gutierrez, Noemi A., Maddox, Ian S. Role of Chemotaxis in Solvent Production by Clostridium acetobutylicum Appl. (jakr-brno.cz)
Enzyme3
- The corresponding enzyme activities, such as NAD(P)H-dependent peroxide (H 2 O 2 and alkyl hydroperoxides) reductase, were highly induced, indicating that microoxic growth of C. acetobutylicum is associated with the expression of a number of genes for oxygen metabolism and ROS scavenging. (asm.org)
- SUMMARY: The hydrogenase enzyme of Clostridium acetobutylicum plays a pivotal role in controlling electron flow, and hence carbon flow, during the complex biphasic fermentation of carbohydrates to the neutral solvents acetone and butanol. (microbiologyresearch.org)
- We have reported the 3D structure of C. acetobutylicum thiolase-a key enzyme involved in n-butanol biosynthesis, for the first time. (eurekalert.org)
P2621
- We report here the cloning and molecular characterization of the hydrogenase-encoding gene (hydA) from C. acetobutylicum P262. (microbiologyresearch.org)
Anaerobe1
- The transport of glucose by the solventogenic anaerobe Clostridium acetobutylicum was investigated. (napier.ac.uk)
Characterization2
- Characterization of the cellulolytic complex (cellulosome) of Clostridium acetobutylicum. (semanticscholar.org)
- Characterization of the cellulose-binding domain of the Clostridium cellulovorans cellulose-binding protein A. (semanticscholar.org)
Hydrogenase protein product1
- This C. acetobutylicum hydrogenase protein product has 82% similarity and 67% identity with the C. pasteurianum hydrogenase-1 protein. (microbiologyresearch.org)
Xylose1
- In this system, C. acetobutylicum produced 9.60 g/L ABE from CFAX and xylose. (ingentaconnect.com)
Solventogenesis3
- The relationship between solventogenesis, heat shock, and DNA topology in Clostridium acetobutylicum was investigated by inhibiting DNA gyrase in vivo by high concentrations of novobiocin. (semanticscholar.org)
- Transcriptional regulation of solventogenesis in Clostridium acetobutylicum. (semanticscholar.org)
- RNA was isolated from the different morphological and physiological stages of a batch C. acetobutylicum fermentation, and further Northern blot analyses revealed no differences in the expression of the gene during acidogenesis as opposed to solventogenesis. (microbiologyresearch.org)
Production21
- EPA Clostridium acetobutylicum Final Risk Assessment Genetic Engineering of Clostridium acetobutylicum for Enhanced Production of Hydrogen Gas: Penn State University. (wikipedia.org)
- Butyric acid production from spent coffee grounds by engineered Clostridium tyrobutyricum overexpressing galactose catabolism genes. (jakesonline.org)
- The nucleotide sequence of three open reading frames in the acetone-production locus of Clostridium acetobutylicum ATCC824 has been established. (nih.gov)
- Carbon monoxide sparged in batch fermentations of C. acetobutylicum inhibits the production of H 2 by the hydrogenase and enhances the production of solvents by making available larger amounts of NAD(P)H 2 to the cells. (springer.com)
- By using recently developed tools for genetic manipulation of Clostridia, we up-regulated and down-regulated expression of enzymes involved in the carbohydrate fermentation to improve production of solvents. (aiche.org)
- The ABE method devi… Butanol Production from Corn Fiber Xylan Using Clostridium acetobutylicum. (jakr-brno.cz)
- Using response surface method, 20 runs of experiments were carried out to investigate the effects of initial pH variation (5-8), temperature (25-40°C), and glucose concentration (4-12 g/l) on biohydrogen production in dark fermentation method by Clostridium acetobutylicum (PTCC 1492). (alertamigratoria.org)
- May 22, 2020 · Liu D, Chen Y, Ding FY et al (2014) Biobutanol production in a Clostridium acetobutylicum biofilm reactor integrated with simultaneous product recovery by adsorption. (casablanca-kamyk.pl)
- He, X.J., Han-Jie Ying, H.J.: Biobutanol production in a Clostridium acetobutylicum biofilm reactor integrated with simultaneous product recovery by adsorption. (casablanca-kamyk.pl)
- Improved n-butanol production by a non-acetone producing Clostridium pasteurianum DSMZ 525 in mixed substrate fermentation. (casablanca-kamyk.pl)
- Biobutanol production in a Clostridium acetobutylicum biofilm reactor. (casablanca-kamyk.pl)
- Butanol Production from Corn Fiber Xylan Using Clostridium acetob. (ingentaconnect.com)
- This study conducted batch experiments to evaluate the potential of butanol production from microalgae biodiesel residues by Clostridium acetobutylicum. (ncku.edu.tw)
- Clostridium acetobutylicum is well-known for its butanol production. (omicsdi.org)
- In this study, ABE production from the fermentation of PKC using Clostridium acetobutylicum YM1 in a batch culture was conducted. (ncsu.edu)
- This study showed that the pretreatment of PKC improved the content of fermentable sugars and subsequently enhanced the production of ABE by C. acetobutylicum YM1. (ncsu.edu)
- Induced production, synthesis and immunomodulatory action of clostrisulfone, a diarylsulfone from Clostridium acetobutylicum. (leibniz-hki.de)
- Therefore, a novel ABE fermentation strategy focusing on bio-acetone production by co-culturing Clostridium acetobutylicum/Saccharomyces cerevisiae with exogenous acetate addition was proposed. (usda.gov)
- Anaerobic production of poly (3-hydroxybutyrate)(PHB) and its precursor 3-hydroxybutyrate (3-HB) from synthesis gas by autotrophic clostridia. (uni-ulm.de)
- Biological n-butanol production was first reported by Louis Pasteur in 1861, and the bioprocess was industrialized using Clostridium acetobutylicum . (eurekalert.org)
- The first, Clostridium tyrobutyricum , optimizes the production of hydrogen and butyric acid, while the other, Clostridium acetobutylicum , converts the butyric acid to butanol. (greencarcongress.com)
Pasteurianum1
- C. acetobutylicum hydrogenase product matches Clostridium pasteurianum hydrogenase-1 protein in 67% of its sequence and resembles it in 82% of the sequence. (readabstracts.com)
NCBI1
- Your access to the NCBI website at www.ncbi.nlm.nih.gov has been Clostridium acetobutylicum using lignocellulosic biomass as a substrate could further enlarge the application scope of butanol fermentation. (jakr-brno.cz)
Proteins3
- Analysis of the genome of Clostridium acetobutylicum has revealed three genes encoding Fur-like proteins. (omicsdi.org)
- Direct phosphorylation of Spo0A by histidine kinases and dephosphorylation by kinase-like proteins may be a common feature of the clostridia that may represent the ancestral state before the great oxygen event some 2.4 billion years ago, after which additional phosphorelay proteins were recruited in the evolutionary lineage that led to the bacilli. (aber.ac.uk)
- Findings from the new study-published recently in mBio through an article titled " Interspecies Microbial Fusion and Large-Scale Exchange of Cytoplasmic Proteins and RNA in a Syntrophic Clostridium Coculture "-has the potential to shed light on unexplained phenomena affecting human health, energy research, biotechnology, and more. (genengnews.com)
Metabolism4
- New insights into the butyric acid metabolism of Clostridium acetobutylicum. (jakesonline.org)
- Transcriptional analysis was performed on Clostridium acetobutylicum with the goal of identifying sugar-specific mechanisms for the transcriptional regulation of transport and metabolism genes. (microbiologyresearch.org)
- Whereas genome mining points to a high potential for secondary metabolism in C. acetobutylicum , the functions of most biosynthetic gene clusters are cryptic. (leibniz-hki.de)
- Did evolutionary processes really have the ability or forward-thinking insight to construct the metabolism in C. acetobutylicum ? (icr.org)
Bacillus2
- Clostridium acetobutylicum is a Gram-positive bacillus (1). (kenyon.edu)
- Clostridium acetobutylicum is a Gram-positive bacillus (1).C. The butyric acid titer, BA/AA ratio, and productivity obtained in this study were the highest values reported for C. acetobutylicum, and the BA/AA ratio and productivity were also comparable to those of native butyric acid producer Clostridium tyrobutyricum. (jakesonline.org)
Redox balance1
- this then leads to the eventual inability of clostridia to maintain their internal redox balance ( 51 , 55 ). (asm.org)
Molecular1
- In a previous work, we demonstrated that C. acetobutylicum produced an inactive cellulosome with an apparent molecular mass of 665 kDa ( 14 ). (asm.org)