A species of gram-positive bacteria in the family Clostridiaceae, used for the industrial production of SOLVENTS.
A genus of motile or nonmotile gram-positive bacteria of the family Clostridiaceae. Many species have been identified with some being pathogenic. They occur in water, soil, and in the intestinal tract of humans and lower animals.
Isomeric forms and derivatives of butanol (C4H9OH).
A colorless liquid used as a solvent and an antiseptic. It is one of the ketone bodies produced during ketoacidosis.
A common inhabitant of the colon flora in human infants and sometimes in adults. It produces a toxin that causes pseudomembranous enterocolitis (ENTEROCOLITIS, PSEUDOMEMBRANOUS) in patients receiving antibiotic therapy.
An enzyme that catalyzes the synthesis of acetylphosphate from acetyl-CoA and inorganic phosphate. Acetylphosphate serves as a high-energy phosphate compound. EC 2.3.1.8.
A four carbon linear hydrocarbon that has a hydroxy group at position 1.
Enzymes which transfer coenzyme A moieties from acyl- or acetyl-CoA to various carboxylic acceptors forming a thiol ester. Enzymes in this group are instrumental in ketone body metabolism and utilization of acetoacetate in mitochondria. EC 2.8.3.
A species of gram-positive bacteria in the family Clostridiaceae, capable of solventogenesis, and isolated from SOIL, infected WOUNDS, fermenting OLIVES, and spoiled CANDY.
Infections with bacteria of the genus CLOSTRIDIUM.
Anaerobic degradation of GLUCOSE or other organic nutrients to gain energy in the form of ATP. End products vary depending on organisms, substrates, and enzymatic pathways. Common fermentation products include ETHANOL and LACTIC ACID.
A class of enzymes that transfers phosphate groups and has a carboxyl group as an acceptor. EC 2.7.2.
A class of iron-sulfur proteins that contains one iron coordinated to the sulfur atom of four cysteine residues. (McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Any of the processes by which cytoplasmic or intercellular factors influence the differential control of gene action in bacteria.
A species of anaerobic, gram-positive, rod-shaped bacteria in the family Clostridiaceae that produces proteins with characteristic neurotoxicity. It is the etiologic agent of BOTULISM in humans, wild fowl, HORSES; and CATTLE. Seven subtypes (sometimes called antigenic types, or strains) exist, each producing a different botulinum toxin (BOTULINUM TOXINS). The organism and its spores are widely distributed in nature.
Type species of the genus CLOSTRIDIUM, a gram-positive bacteria in the family Clostridiaceae. It is used as a source of PROBIOTICS.
Proteins found in any species of bacterium.
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
A non-heme iron protein consisting of eight apparently identical subunits each containing 2 iron atoms. It binds one molecule of oxygen per pair of iron atoms and functions as a respiratory protein.
An enzyme found in bacteria. It catalyzes the reduction of FERREDOXIN and other substances in the presence of molecular hydrogen and is involved in the electron transport of bacterial photosynthesis.
Heat and stain resistant, metabolically inactive bodies formed within the vegetative cells of bacteria of the genera Bacillus and Clostridium.
The functional hereditary units of BACTERIA.
An enzyme that catalyzes reversibly the phosphorylation of acetate in the presence of a divalent cation and ATP with the formation of acetylphosphate and ADP. It is important in the glycolysis process. EC 2.7.2.1.
Extracellular structures found in a variety of microorganisms. They contain CELLULASES and play an important role in the digestion of CELLULOSE.
A product of fermentation. It is a component of the butanediol cycle in microorganisms. In mammals it is oxidized to carbon dioxide.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
Hydrocarbon-rich byproducts from the non-fossilized BIOMASS that are combusted to generate energy as opposed to fossilized hydrocarbon deposits (FOSSIL FUELS).
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Derivatives of BUTYRIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxypropane structure.
An acute inflammation of the INTESTINAL MUCOSA that is characterized by the presence of pseudomembranes or plaques in the SMALL INTESTINE (pseudomembranous enteritis) and the LARGE INTESTINE (pseudomembranous colitis). It is commonly associated with antibiotic therapy and CLOSTRIDIUM DIFFICILE colonization.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Techniques to alter a gene sequence that result in an inactivated gene, or one in which the expression can be inactivated at a chosen time during development to study the loss of function of a gene.
Oxidoreductases that are specific for ALDEHYDES.
A four carbon acid, CH3CH2CH2COOH, with an unpleasant odor that occurs in butter and animal fat as the glycerol ester.
Deoxyribonucleic acid that makes up the genetic material of bacteria.
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A zinc-containing enzyme which oxidizes primary and secondary alcohols or hemiacetals in the presence of NAD. In alcoholic fermentation, it catalyzes the final step of reducing an aldehyde to an alcohol in the presence of NADH and hydrogen.
An enzyme that catalyzes the formation of acetoacetyl-CoA from two molecules of ACETYL COA. Some enzymes called thiolase or thiolase-I have referred to this activity or to the activity of ACETYL-COA C-ACYLTRANSFERASE.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
A species of gram-positive, thermophilic, cellulolytic bacteria in the family Clostridaceae. It degrades and ferments CELLOBIOSE and CELLULOSE to ETHANOL in the CELLULOSOME.
A species of gram-positive bacteria in the family Clostridiaceae. It is a cellulolytic, mesophilic species isolated from decayed GRASS.
Enzymes that catalyze the addition of a carboxyl group to a compound (carboxylases) or the removal of a carboxyl group from a compound (decarboxylases). EC 4.1.1.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
RNA molecules which hybridize to complementary sequences in either RNA or DNA altering the function of the latter. Endogenous antisense RNAs function as regulators of gene expression by a variety of mechanisms. Synthetic antisense RNAs are used to effect the functioning of specific genes for investigative or therapeutic purposes.
The cause of TETANUS in humans and domestic animals. It is a common inhabitant of human and horse intestines as well as soil. Two components make up its potent exotoxin activity, a neurotoxin and a hemolytic toxin.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.
Ribonucleic acid in bacteria having regulatory and catalytic roles as well as involvement in protein synthesis.
Derivatives of ACETIC ACID. Included under this heading are a broad variety of acid forms, salts, esters, and amides that contain the carboxymethane structure.
Complex sets of enzymatic reactions connected to each other via their product and substrate metabolites.
The genetic complement of a BACTERIA as represented in its DNA.
The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.
Toxic proteins produced from the species CLOSTRIDIUM BOTULINUM. The toxins are synthesized as a single peptide chain which is processed into a mature protein consisting of a heavy chain and light chain joined via a disulfide bond. The botulinum toxin light chain is a zinc-dependent protease which is released from the heavy chain upon ENDOCYTOSIS into PRESYNAPTIC NERVE ENDINGS. Once inside the cell the botulinum toxin light chain cleaves specific SNARE proteins which are essential for secretion of ACETYLCHOLINE by SYNAPTIC VESICLES. This inhibition of acetylcholine release results in muscular PARALYSIS.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
A species of gram-positive bacteria in the family Clostridiaceae, found in INTESTINES and SOIL.
A clear, colorless liquid rapidly absorbed from the gastrointestinal tract and distributed throughout the body. It has bactericidal activity and is used often as a topical disinfectant. It is widely used as a solvent and preservative in pharmaceutical preparations as well as serving as the primary ingredient in ALCOHOLIC BEVERAGES.
Toxic substances formed in or elaborated by bacteria; they are usually proteins with high molecular weight and antigenicity; some are used as antibiotics and some to skin test for the presence of or susceptibility to certain diseases.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.

Intracellular butyryl phosphate and acetyl phosphate concentrations in Clostridium acetobutylicum and their implications for solvent formation. (1/98)

It has been suggested (L. H. Harris, R. P. Desai, N. E. Welker, and E. T. Papoutsakis, Biotechnol. Bioeng. 67:1-11, 2000) that butyryl phosphate (BuP) is a regulator of solventogenesis in Clostridium acetobutylicum. Here, we determined BuP and acetyl phosphate (AcP) levels in fermentations of C. acetobutylicum wild type (WT), degenerate strain M5, a butyrate kinase (buk) mutant, and a phosphotransacetylase (pta) mutant. A sensitive method was developed to measure BuP and AcP in the same sample. Compared to the WT, the buk mutant had higher levels of BuP and AcP; the BuP levels were high during the early exponential phase, and there was a peak corresponding to solvent production. Consistent with this, solvent formation was initiated significantly earlier and was much stronger in the buk mutant than in all other strains. For all strains, initiation of butanol formation corresponded to a BuP peak concentration that was more than 60 to 70 pmol/g (dry weight), and higher and sustained levels corresponded to higher butanol formation fluxes. The BuP levels never exceeded 40 to 50 pmol/g (dry weight) in strain M5, which produces no solvents. The BuP profiles were bimodal, and there was a second peak midway through solventogenesis that corresponded to carboxylic acid reutilization. AcP showed a delayed single peak during late solventogenesis corresponding to acetate reutilization. As expected, in the pta mutant the AcP levels were very low, yet this strain exhibited strong butanol production. These data suggest that BuP is a regulatory molecule that may act as a phosphodonor of transcriptional factors. DNA array-based transcriptional analysis of the buk and M5 mutants demonstrated that high BuP levels corresponded to downregulation of flagellar genes and upregulation of solvent formation and stress genes.  (+info)

SpoIIE regulates sporulation but does not directly affect solventogenesis in Clostridium acetobutylicum ATCC 824. (2/98)

Using gene expression reporter vectors, we examined the activity of the spoIIE promoter in wild-type and spo0A-deleted strains of Clostridium acetobutylicum ATCC 824. In wild-type cells, the spoIIE promoter is active in a transient manner during late solventogenesis, but in strain SKO1, where the sporulation initiator spo0A is disrupted, no spoIIE promoter activity is detectable at any stage of growth. Strains 824(pMSpo) and 824(pASspo) were created to overexpress spoIIE and to decrease spoIIE expression via antisense RNA targeted against spoIIE, respectively. Some cultures of strains 824(pMSpo) degenerated during fermentations by losing the pSOL1 megaplasmid and hence did not produce the solvents ethanol, acetone, and butanol. The frequent degeneration event was shown to require an intact copy of spoIIE. Nondegenerate cultures of 824(pMSpo) exhibited normal growth and solvent production. Strain 824(pASspo) exhibited prolonged solventogenesis characterized by increased production of ethanol (225%), acetone (43%), and butanol (110%). Sporulation in strains harboring pASspo was significantly delayed, with sporulating cells exhibiting altered morphology. These results suggest that SpoIIE has no direct effect on the control of solventogenesis and that the changes in solvent production in spoIIE-downregulated cells are mediated by effects on the cell during sporulation.  (+info)

Heterologous production, assembly, and secretion of a minicellulosome by Clostridium acetobutylicum ATCC 824. (3/98)

The gene man5K encoding the mannanase Man5K from Clostridium cellulolyticum was cloned alone or as an operon with the gene cipC1 encoding a truncated scaffoldin (miniCipC1) of the same origin in the solventogenic Clostridium acetobutylicum. The expression of the heterologous gene(s) was under the control of a weakened thiolase promoter Pthl. The recombinant strains of the solventogenic bacterium were both found to secrete active Man5K in the range of milligrams per liter. In the case of the strain expressing only man5K, a large fraction of the recombinant enzyme was truncated and lost the N-terminal dockerin domain, but it remained active towards galactomannan. When man5K was coexpressed with cipC1 in C. acetobutylicum, the recombinant strain secreted almost exclusively full-length mannanase, which bound to the scaffoldin miniCipC1, thus showing that complexation to the scaffoldin stabilized the enzyme. The secreted heterologous complex was found to be functional: it binds to crystalline cellulose via the carbohydrate binding module of the miniscaffoldin, and the complexed mannanase is active towards galactomannan. Taken together, these data show that C. acetobutylicum is a suitable host for the production, assembly, and secretion of heterologous minicellulosomes.  (+info)

Expression of abrB310 and SinR, and effects of decreased abrB310 expression on the transition from acidogenesis to solventogenesis, in Clostridium acetobutylicum ATCC 824. (4/98)

The transcription factors sinR and abrB are involved in the control of sporulation initiation in Bacillus subtilis. We identified a single homologue to sinR and three highly similar homologues to abrB, designated abrB310, abrB1941, and abrB3647, in Clostridium acetobutylicum ATCC 824. Using reporter vectors, we showed that the promoters of abrB1941 and abrB3647 were not active under the growth conditions tested. The abrB310 promoter was strongly active throughout growth and exhibited a transient elevation of expression at the onset of solventogenesis. Primer extension assays showed that two transcripts of abrB310 and a single, extremely weak transcript for sinR are expressed. Potential -35 and -10 consensus motifs are readily identifiable surrounding the transcription start sites of abrB310 and sinR, with a single putative 0A box present within the promoter of abrB310. In strains of C. acetobutylicum transformed with plasmids to elevate sinR expression or decrease sinR expression, no significant differences in growth or in acid or solvent production were observed compared to the control strains. In C. acetobutylicum strain 824(pAS310), which expressed an antisense RNA construct targeted against abrB310, the acids acetate and butyrate accumulated to approximately twice the normal concentration. This accumulation corresponded to a delay and decrease in acetone and butanol production. It was also found that sporulation in strain 824(pAS310) was delayed but that the morphology of sporulating cells and spores was normal. Based upon these observations, we propose that abrB310 may act as a regulator at the transition between acidogenic and solventogenic growth.  (+info)

Homologous and heterologous overexpression in Clostridium acetobutylicum and characterization of purified clostridial and algal Fe-only hydrogenases with high specific activities. (5/98)

Clostridium acetobutylicum ATCC 824 was selected for the homologous overexpression of its Fe-only hydrogenase and for the heterologous expressions of the Chlamydomonas reinhardtii and Scenedesmus obliquus HydA1 Fe-only hydrogenases. The three Strep tag II-tagged Fe-only hydrogenases were isolated with high specific activities by two-step column chromatography. The purified algal hydrogenases evolve hydrogen with rates of around 700 micromol H(2) min(-1) mg(-1), while HydA from C. acetobutylicum (HydA(Ca)) shows the highest activity (5,522 micromol H(2) min(-1) mg(-1)) in the direction of hydrogen uptake. Further, kinetic parameters and substrate specificity were reported. An electron paramagnetic resonance (EPR) analysis of the thionin-oxidized HydA(Ca) protein indicates a characteristic rhombic EPR signal that is typical for the oxidized H cluster of Fe-only hydrogenases.  (+info)

Genomic analysis of the protein secretion systems in Clostridium acetobutylicum ATCC 824. (6/98)

Consistent information about protein secretion in Gram-positive bacteria is essentially restricted to the model organism Bacillus subtilis. Among genome-sequenced clostridia, Clostridium acetobutylicum has been the most extensively studied from a physiological point of view and is the organism for which the largest variety of molecular biology tools have been developed. Following in silico analyses, both secreted proteins and protein secretion systems were identified. The Tat (Twin arginine translocation; TC #2.A.64) pathway and ABC (ATP binding cassette) protein exporters (TC #3.A.1.) could not be identified, but the Sec (secretion) pathway (TC #3.A.5) appears to be used prevalently. Similarly, a flagella export apparatus (FEA; TC #3.A.6.), holins (TC #1.E.), and an ESAT-6/WXG100 (early secreted antigen target of 6 kDa/proteins with a WXG motif of approximately 100 residues) secretion system were identified. Here, we report for the first time the identification of a fimbrilin protein exporter (FPE; TC #3.A.14) and a Tad (tight adherence) export apparatus in C. acetobutylicum. This investigation highlights the potential use of this saprophytic bacterium in biotechnological and biomedical applications as well as a model organism for studying protein secretion in pathogenic Gram-positive bacteria.  (+info)

Diffusion, mixing, and associated dye effects in DNA-microarray hybridizations. (7/98)

Typical DNA microarrays utilize diffusion of dye-labeled cDNA probes followed by sequence-specific hybridization to immobilized targets. Here we experimentally estimated the distance typical probes travel during static 16-h hybridizations. Probes labeled with Cy3 and Cy5 were individually introduced to opposite sides of a microarray with minimal convective mixing. Oppositely labeled probes diffused across the initial front separating the two solutions, generating a zone with both dyes present. Diffusion-distance estimates for Cy3- and Cy5-labeled cDNAs were 3.8 mm and 2.6 mm, respectively, despite having almost identical molecular masses. In separate 16-h hybridization experiments with oppositely labeled probes premixed, arrays that were continuously mixed had 15-20% higher signal intensities than arrays hybridized statically. However, no change was observed in the Cy3/Cy5 signal intensity ratio between continuously mixed and static hybridizations. This suggests that the observed dye bias in diffusion-distance estimates results from differences in the detection limits of Cy3 and Cy5-labeled cDNA, a potential concern for array data on low-abundance transcripts. Our conservative diffusion-distance estimates indicate that replicate targets >7.6 mm apart will not compete for scarce probes. Also, raising the microarray gap height would delay the onset of diffusion-limited hybridization by increasing the amount of available probe.  (+info)

Transcriptional program of early sporulation and stationary-phase events in Clostridium acetobutylicum. (8/98)

DNA microarray analysis of Clostridium acetobutylicum was used to examine the genomic-scale gene expression changes during the shift from exponential-phase growth and acidogenesis to stationary phase and solventogenesis. Self-organizing maps were used to identify novel expression patterns of functional gene classes, including aromatic and branched-chain amino acid synthesis, ribosomal proteins, cobalt and iron transporters, cobalamin biosynthesis, and lipid biosynthesis. The majority of pSOL1 megaplasmid genes (in addition to the solventogenic genes aad-ctfA-ctfB and adc) had increased expression at the onset of solventogenesis, suggesting that other megaplasmid genes may play a role in stationary-phase phenomena. Analysis of sporulation genes and comparison with published Bacillus subtilis results indicated conserved expression patterns of early sporulation genes, including spo0A, the sigF operon, and putative canonical genes of the sigma(H) and sigma(F) regulons. However, sigE expression could not be detected within 7.5 h of initial spo0A expression, consistent with the observed extended time between the appearance of clostridial forms and endospore formation. The results were compared with microarray comparisons of the wild-type strain and the nonsolventogenic, asporogenous M5 strain, which lacks the pSOL1 megaplasmid. While some results were similar, the expression of primary metabolism genes and heat shock proteins was higher in M5, suggesting a difference in metabolic regulation or a butyrate stress response in M5. The results of this microarray platform and analysis were further validated by comparing gene expression patterns to previously published Northern analyses, reporter assays, and two-dimensional protein electrophoresis data of metabolic genes (including all major solventogenesis genes), sporulation genes, heat shock proteins, and other solventogenesis-induced gene expression.  (+info)

Thiolase of Clostridium acetobutylicum is an important enzyme involved in both, acid and solvent fermentation. Two thiolase genes (thlA and thlB) have been cloned and sequenced from Clostridium acetobutylicum DSM 792, showing high homology to each other and to thiolases of PHA-synthesizing bacteria. The thlA gene is identical to the gene already cloned and sequenced from strain ATCC 824 (Stim- Herndon et al., 1995, Gene 154: 81-85). Using primer extension and S1 nuclease analysis a transcriptional start site was identified 102 bp upstream of the thlA start codon. This site was preceded by a region that exhibits high similarity to the s70 consensus promoter sequences of Gram-positive and -negative bacteria. Regulation of thlA and thlB was studied at the transcriptional level to elucidate the specific function of each gene. Non-radioactive primer extension analysis using fluorescein-labelled oligonucleotides and Northern blot analysis revealed high levels of thlA transcripts in acid- and ...
Previously we have developed a butanol tolerant mutant of Clostridium acetobutylicum Rh8, from the wild type strain DSM 1731. Strain Rh8 can tolerate up to 19 g/L butanol, with solvent titer improved accordingly, thus exhibiting industrial application potential. To test if strain Rh8 can be used for production of high level mixed alcohols, a single secondary alcohol dehydrogenase from Clostridium beijerinckii NRRL B593 was overexpressed in strain Rh8 under the control of thl promoter. The heterogenous gene sADH was functionally expressed in C. acetobutylicum Rh8. This simple, one-step engineering approach switched the traditional ABE (acetone-butanol-ethanol) fermentation to IBE (isopropanol-butanol-ethanol) fermentation. The total alcohol titer reached 23.88 g/l (7.6 g/l isopropanol, 15 g/l butanol, and 1.28 g/l ethanol) with a yield to glucose of 31.42%. The acid (butyrate and acetate) assimilation rate in isopropanol producing strain Rh8(psADH) was increased. The improved butanol tolerance and the
CRISPR/Cas-based genetic engineering has revolutionised molecular biology in both eukaryotes and prokaryotes. Several tools dedicated to the genomic transformation of the Clostridium genus of Gram-positive bacteria have been described in the literature; however, the integration of large DNA fragments still remains relatively limited. In this study, a CRISPR/Cas9 genome editing tool using a two-plasmid strategy was developed for the solventogenic strain Clostridium acetobutylicum ATCC 824. Codon-optimised cas9 from Streptococcus pyogenes was placed under the control of an anhydrotetracycline-inducible promoter on one plasmid, while the gRNA expression cassettes and editing templates were located on a second plasmid. Through the sequential introduction of these vectors into the cell, we achieved highly accurate genome modifications, including nucleotide substitution, gene deletion and cassette insertion up to 3.6 kb. To demonstrate its potential, this genome editing tool was used to generate a marker-free
One of the biggest limitations in the study and engineering of anaerobic Clostridium organisms is the lack of strong fluorescent reporters capable of strong and real-time fluorescence. Recently, we developed a strong fluorescent reporter system for Clostridium organisms based on the FAST protein. Here, we report the development of two new strong fluorescent reporter systems for Clostridium organisms based on the HaloTag and SNAP-tag proteins, which produce strong fluorescent signals when covalently bound to fluorogenic ligands. These new fluorescent reporters are orthogonal to the FAST ligands and to each other, allowing for simultaneous labeling and visualization. We used HaloTag and SNAP-tag to label the strictly anaerobic organisms Clostridium acetobutylicum and Clostridium ljungdahlii. We have also identified a new strong promoter for protein expression in C. acetobutylicum, based on the phosphotransacetylase gene (pta) from C. ljungdahlii. Furthermore, the HaloTag and the SNAP-tag, in ...
Clostridium acetobutylicum, ATCC 824, is a commercially valuable bacterium sometimes called the Weizmann Organism, after Jewish-Russian-born Chaim Weizmann. A senior lecturer at the University of Manchester, England, he used them in 1916 as a bio-chemical tool to produce at the same time, jointly, acetone, ethanol, and butanol from starch. The method has been described since as the ABE process, (Acetone Butanol Ethanol fermentation process), yielding 3 parts of acetone, 6 of butanol, and 1 of ethanol. Acetone was used in the important wartime task of casting cordite. The alcohols were used to produce vehicle fuels and synthetic rubber. Unlike yeast, which can digest only sugar into alcohol and carbon dioxide, C. acetobutylicum and other Clostridia can digest whey, sugar, starch, cellulose and perhaps certain types of lignin, yielding butanol, propionic acid, ether, and glycerin. ...
Clostridium acetobutylicum ATCC 824 is a commercially valuable bacterium sometimes called the Weizmann Organism after JewishRussianborn Chaim Weizmann
Production of Acetone, Butanol, and Ethanol (ABE) by Clostridium acetobutylicum YM1 from Pretreated Palm Kernel Cake in Batch Culture Fermentation
Iron reduction in Gram-positive bacteria is not well understood yet, even if it has been investigated in some extent for Gram-positive bacteria. The mechanisms involved in the delivery of electrons to a solid terminal electron acceptor like iron oxides have not been defined. Clostridium acetobutylicum is an appropriate Gram-positive bacterium to study those mechanisms as genetic tools have been developed due to its industrial interest, allowing easy targeted and random mutagenesis. In this Masters project, phenotype of two mutants, whose dihydroorotate dehydrogenase 1B (pyrD) or ferredoxin hydrogenase (hydA) gene have been knocked out through ClosTron mutagenesis, have been characterized and no phenotype diverging from the wild strain has been detected. However, evidences of flavin presence in the spent growth medium have been observed during experiment. An attempt to measure their redox state, either by direct measurement or through the addition of AQDS, has been done but results are ...
This model represents a small family of proteins homologous (and likely functionally equivalent to) aconitase 1. Members are found, so far in the anaerobe Clostridium acetobutylicum, in the microaerophilic, early-branching bacterium Aquifex aeolicus, and in the halophilic archaeon Halobacterium sp. NRC-1. No member is experimentally characterized ...
Signal transduction proteins, Histidine Kinase, Response regulator, Phophotransfer protein, HPT, Phosphorelay, complete metagenomes browser, TCS, Prokaryotic Two-Component Systems database, P2CS, annotation browser, MIST, SENTRA
The genetic study of the clostridia is in its infancy, but significant advances have been made in recent years. C. acetobutylicum was the most reported in acetone-butanol-ethanol (ABE) fermentation for synthesis of biobutanol with higher yields [5, 6]. Cells were stained in an iodine solution. Currently, petroleum-based products have largely replaced these fermentation processes. Clostridium strains with the potential of utilizing various biomass (e.g., corn cobs, cassava and rice bran) and the production of biofuels (e.g., butanol) were mainly classified within Clade 1 and Clade 5 such as C. cellulovorans 743B and C. saccharoperbutylacetonicum N1-4 [39,40,41].Genomes from the same clustered group usually appear to have similar metabolic functions, which … It was formerly used from the First World War onwards on an industrial … Although most plasmids encode unknown (cryptic) functions, some have been demonstrated to possess genes for virulence and antibiotic resistance. NNT: The enzymes ...
Influence of sulfate on the anaerobic hydrolysis and acidogenesis of particulate organics was studied in a completely mixed acidogenic reactor. The shattered food waste as a particulate organics was added into the reactor at a daily basis while dilution water containing various concentration of sulfate was continuously supplied. The efficiencies of hydrolysis and acidogenesis at control were 60.1% and 19.4%, respectively while they have increased up to 70.6% and 46.2% respectively at 350 mg/L of the sulfate level in the dilution water. The buffering capacity of the acidogenic system increased with the increase of the sulfate level. The efficiencies of hydrolysis and acidogenesis gradually decreased at over 350 mg/L of the sulfate level in the dilution water. It was caused by the increase of the acidogenesis product used as electron donor for sulfate reduction and inhibition effect of the hydrogen sulfide from sulfate reduction. It could be concluded that sulfate in acidogenesis of particulate ...
As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
1. Meynial-Salles I., Cervin M. A. and Soucaille P. 2005. New tool for metabolic pathway engineering in E. coli : one step method to modulate the expression of chromosomal genes : Appl. Environ. Microbiol., 71, 2140-2144. (impact Factor 2007 4.004 from J C Rt). 2. Girbal L., Von AbendrothG., WinklerM., BentonP. M. C., Meynial-SallesI., Croux C., PetersJ., Happe T. and Soucaille P. 2005. Homologous/heterologous over-expression in Clostridium acetobutylicum and characterization of purified clostridial and algal Fe-only hydrogenases with high specific activity : Appl. Environ. Microbiol., 71, 2777-2781. (impact Factor 2007 4,004 from J C R). 3. Meynial-Salles I., Gonzalez-Pajuelo M., , Mendes P., Andrade J. C., Vasconcelos I. and Soucaille P. 2005. Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1.3 propanediol from glycerol : Met. Eng., 7, 329-336. (impact Factor 2007 3.444 from J C R). 4. Gonzalez-Pajuelo M., Meynial-Salles I., Mendes P., Soucaille P. and ...
ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.
p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.,/p> ,p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.,/p> ,p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).,/p> ,p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x,sup>64,/sup> + x,sup>4,/sup> + x,sup>3,/sup> + x + 1. The algorithm is described in the ISO 3309 standard. ,/p> ,p class=publication>Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.,br /> ,strong>Cyclic redundancy and other checksums,/strong>,br /> ,a href=http://www.nrbook.com/b/bookcpdf.php>Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993),/a>),/p> Checksum:i ...
The production of biohydrogen from rice mill wastes, including rice bran and rice mill effluent by Clostridium acetobutylicum NCIM 2877 was investigated in a batch culture system and optimized the temperature and pH conditions. At 35 °C with initial pH of 5.2 a yield of 55.7±0.8 ml with 88.6 ± 0.3% substrate utilization and at pH 6 the production was 68.7 ± 0.9 ml with 85 ± 1.0 % substrate utilization. Addition of metal ions resulted in better yield and substrate utilization efficiency of the bacteria. FeSO4.7H2O at a concentration of 50 mg/l gave 79.7 ± 1.5 ml with 96% of substrate utilization. Cobalt effected the production greatly by giving 96.3 ± 0.9 ml with 95.3 ± 0.7% of substrate utilization whereas Nickel didnt showed much effective results by giving only a maximum of 74.7 ± 1.5 ml production at 25 mg/l concentration. Conclusively, it can be stated that rice mill wastes can be used as a substrate and use of metal ions will play a key role in the enhancement of biohydrogen ...
SWISS-MODEL Repository entry for Q97EW9 (RNM5_CLOAB), Ribonuclease M5. Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710/ VKM B-1787)
Task---- I ask Dr. Diego Gonzále Halphen if he could give us clhamydomonas reinhardtii. I ask Jovita Martínez, who take care of the strain collection of CINVESTAV-Mexico, if she could give us clostridium acetobutylicum. ...
Numerous significant hits in gapped BLAST to conserved hypothetical sequences; residues 13-374 are 34% similar to (AF036764) unknown [Clostridium acetobutylicum]; and residues 15-392 are 30% similar to YQEV_BACSU ...
Started in 1979 under license of UOP - USA. It designed to produce hexane (Food Grade) which is used for extraction food oil from plant seeds in addition, it produces many types of important petroleum solvent ...
Sales office :. ALPHADYNAMIC PUMPS (UK) Ltd. Rockleigh House, 37 Burton Road. Ashby De La Zouch - Leicestershire. LE65 2LF - United Kingdom (UK). Tel : +44 1213 680 324, +44 1213 680 472. Email: [email protected]. www.alphadynamicpumps.co.uk. Registered in England and Wales.. Registration No.09706219. Vat No. GB 220393343. ...
The effect of butanol challenge (0, 1.0, 1.5% [vol/vol]) and growth temperature (22, 37, 42°C) on the membrane composition and fluidity of Clostridium acetobutylicum ATCC 824 and a butanol-tolerant mutant, SA-2, was examined in chemically defined medium. Growth of strain ATCC 824 into the stationary phase coincided with a gradual increase in the percent saturated to percent unsaturated (SU) fatty acid ratio. When challenged with butanol at 22 and 37°C, ATCC 824 demonstrated an immediate (within 30 min) dose-response increase in the SU ratio. This strain showed little additional change over a 48-h fermentation. Compared with ATCC 824, growth of SA-2 into the late stationary phase at 22 or 37°C resulted in an overall greater increase in the SU ratio for both unchallenged and challenged cells. This effect was minimized when SA-2 was challenged at 42°C, probably due to the combination of the membrane fluidizing effect of butanol and the elevated temperature. Growth at 42°C resulted in an ...
TY - JOUR. T1 - Single-Amino Acid Modifications Reveal Additional Controls on the Proton Pathway of [FeFe]-Hydrogenase. AU - Cornish, Adam J.. AU - Ginovska, Bojana. AU - Thelen, Adam. AU - Da Silva, Julio C S. AU - Soares, Thereza A.. AU - Raugei, Simone. AU - Dupuis, Michel. AU - Shaw, Wendy J.. AU - Hegg, Eric L.. PY - 2016/6/7. Y1 - 2016/6/7. N2 - The proton pathway of [FeFe]-hydrogenase is essential for enzymatic H2 production and oxidation and is composed of four residues and a water molecule. A computational analysis of this pathway in the [FeFe]-hydrogenase from Clostridium pasteurianum revealed that the solvent-exposed residue of the pathway (Glu282) forms hydrogen bonds to two residues outside of the pathway (Arg286 and Ser320), implying that these residues could function in regulating proton transfer. In this study, we show that substituting Arg286 with leucine eliminates hydrogen bonding with Glu282 and results in an ∼3-fold enhancement of H2 production activity when methyl ...
The fermentation processes to produce propanol and butanol from cellulose are fairly tricky to execute, and the Weizmann organism (Clostridium acetobutylicum) currently used to perform these conversions produces an extremely unpleasant smell, and this must be taken into consideration when designing and locating a fermentation plant. This organism also dies when the butanol content of whatever it is fermenting rises to 7%. For comparison, yeast dies when the ethanol content of its feedstock hits 14%. Specialized strains can tolerate even greater ethanol concentrations - so-called turbo yeast can withstand up to 16% ethanol . However, if ordinary Saccharomyces yeast can be modified to improve its ethanol resistance, scientists may yet one day produce a strain of the Weizmann organism with a butanol resistance higher than the natural boundary of 7%. This would be useful because butanol has a higher energy density than ethanol, and because waste fibre left over from sugar crops used to make ethanol ...
Background Many Firmicutes bacteria, including solvent-producing clostridia such as Clostridium acetobutylicum, are able to utilize xylose, an abundant carbon source in nature. Nevertheless, homology...
ADELPHI, Md. (Feb. 5, 2016) -- Military bases located in remote areas may one day be able to efficiently turn food waste into energy thanks to a collaborative research effort between scientists at the U.S. Army Research Laboratory and the University of Texas at Tyler.. The collaboration, made possible by ARLs Open Campus initiative, specifically focuses on the bacterium known as Clostridium acetobutylicum, which has the ability to convert substances found in food waste to commodity chemicals including butanol, which is useful for fuel cells to produce energy.. Forward operating bases are remote and often hastily constructed without much infrastructure, making waste disposal a significant burden. It is also a significant logistical and financial challenge to transport fuel for vehicles to these FOBs, said Dr. Joshua Banta, assistant biology professor at UT-Tyler who has been working on this project with ARL researchers since 2014. This research project seeks to take that burden and financial ...
Hydrogenases catalyze the formation of hydrogen. The cofactor (H-cluster) of [FeFe]-hydrogenases consists of a [4Fe-4S] cluster bridged to a unique [2Fe] subcluster whose biosynthesis in vivo requires hydrogenase-specific maturases. Here we show that a chemical mimic of the [2Fe] subcluster can reconstitute apo-hydrogenase to full activity, independent of helper proteins. The assembled H-cluster is virtually indistinguishable from the native cofactor. This procedure will be a powerful tool for developing new artificial H-2-producing catalysts.. ...
Ying Zhang, Ines Thiele, Dana Weekes, Zhanwen Li, Lukasz Jaroszewski, Krzysztof Ginalski, Ashley Deacon, John Wooley, Scott Lesley, Ian Wilson, Bernhard Palsson, Andrei Osterman, Adam Godzik. Three-Dimensional Structural View of the Central Metabolic Network of Thermotoga maritima. Science. 2009 Sep 18;325(5947):1544-9.. Alexey M. Eroshkin, Andrew LeBlanc, Dana Weekes, Kai Post, Zhanwen Li, Akhil Rajput, Sal T. Butera, Dennis R. Burton, Adam Godzik. bNAber: database of broadly neutralizing HIV antibodies. Nucl. Acids Res. 2013; published on November 7, 2013.. Grynberg M, Godzik A. NERD: a DNA processing-related domain present in the anthrax virulence plasmid, pXO1. Trends Biochem Sci. 2004 Mar;29(3):106-10 ...
Lui BH, Cochran JR, Swartz, JR. Discovery of improved EGF agonists using a novel in vitro screening platform. J Mol Biol. 2011 Oct 21;413(2):4 06-15. Epub 2011 Aug 23. PMID 21888916 Yang WC, Welsh JP, Lee J, Cooke JP, Swartz JR. Solubility partner IF2 Domain I enables high yield synthesis of transducible transcription factors in Escherichia coli. Protein Expr Purif. 2011 Nov;80(1):145-51. doi: 10.1016/j.pep.2011.06.017. Epub 2011 Jul 2. PMID 21757009 Kuchenreuther JM, George SJ, Grady-Smith CS, Cramer SP, Swartz JR. Cell-free H-cluster synthesis and [FeFe] hydrogenase activation: all five CO and CN⁻ ligands derive from tyrosine. PLoS One (2011);6(5):e20346. Epub 2011 May 31. PMID 21673792 Bingham AS, Smith PR, Swartz JR. Evolution of an [FeFe] hydrogenase with decreased oxygen sensitivity. International Journal of Hydrogen Energy (2011) doi:10.1016/j.ijhydene.2011.02.048 Smith PR, Bingham AS, Swartz JR. Generation of hydrogen from NADPH using an [FeFe] hydrogenase. International Journal of ...
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Genetic information processingProtein fateProtein modification and repair[FeFe] hydrogenase H-cluster maturation GTPase HydF (TIGR03918; HMM-score: 17.7) ...
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arr2 , pspC , sapA , qacEdelta1 , pspD , pspF , sul1 , aacA4-CR , ampC , sul1 , ISCR1 , blaOXA-1 , ampR , pspA , sapC , catB3 , pspB , ...
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Not all vegetables are created equal. Some, like celery, onions, green beans, carrots, lettuces, and melons require a lot more water than other vegetables. Squash, tomatoes, peppers, eggplants, chard, arugula, and dry beans, especially Tepary beans, are better choices for drought gardening, when water is restricted. Most plants have critical periods, when they require more water than normal. This is usually during flowering and fruit production.. If you really want to grow some of the higher-water-need plants, put them together on a separate water valve. That way you can have part of your garden getting more water than the rest, rather than the entire garden getting more water than some plants need. Also, space the plants farther apart, so they arent competing with each other for every precious drop. You may end up with fewer plants, but you wont have to water as much.. The picture above of all the squash is a perfect example of how water-wise gardening can be productive. All of that ...
Increasing demand for the production of renewable fuels has recently generated a particular interest in microbial production of butanol. Anaerobic bacteria, such as Clostridium spp., can naturally convert carbohydrates into a variety of primary products, including alcohols like butanol. The genetics of microorganisms like Clostridium acetobutylicum have been well studied and their solvent-producing metabolic pathways characterized. In contrast, less is known about the genetics of Clostridium spp. capable of converting syngas or its individual components into solvents. In this study, the type of strain of a new solventogenic Clostridium species, C. carboxidivorans, was genetically characterized by genome sequencing. C. carboxidivorans strain P7T possessed a complete Wood-Ljungdahl pathway gene cluster, involving CO and CO2 fixation and conversion to acetyl-CoA. Moreover, with the exception of an acetone production pathway, all the genetic determinants of canonical ABE metabolic pathways for acetate,
Curated}} {{Biorealm Genus}} [[Image:clostridium.ipg.jpg,thumb,400px,right,Clostridium. Courtesy of [http://www-instruct.nmu.edu/cls/lriipi/micro/ Northern Michigan University.]]] ==Classification== ===Higher order taxa:=== Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; Clostridium ===Species:=== Clostridium tetani, Clostridium botulinum, Clostridium perfringens, Clostridium acetobutylicum, Clostridium difficile, Clostridium novyi {, , height=10 bgcolor=#FFDF95 , NCBI: [http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Undef&id=1485&lvl=3&keep=1&srchmode=1&unlock Taxonomy] [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Search&dopt=DocSum&term=txid1485%5BOrganism:exp%5D Genomes] ,} ==Description and Significance== Clostridia are, spore-forming, Gram-positive, anaerobes (although some species are microaerophilic). They are known to produce a variety of toxins, some of which are fatal. ==Genome Structure== Currently there are 3 ...
To increase the butanol titers and selectivity in ,em,Clostridium acetobutylicum,/em, we replaced the promoter of the alcohol/aldehyde dehydrogenase (,em,aad,/em,) gene with the phosphotranbutyrylase (,em,ptb,/em,) promoter and combined this with CoAT downregulation to minimize acetone production. This led to early production of high alcohol (butanol plus ethanol) titers and overall solvent ...
Lease Lab created an E. coli genetic system based on DsrA sRNA to test and retarget these sRNAs to different mRNAs, and succeeded in using it to generate semisynthetic re-targeted sRNAs that simultaneously regulate multiple mRNAs using modular stem-loop antisense regulatory motifs.. Synthetic biology applications of this work include the generation of gene knock-downs in industrially relevant but genetically less-tractable microbes such as Clostridium acetobutylicum, and the fine-tuning of gene expression in fermentation cultures ...
Biosynthesis of the Catalytic H-Cluster of [FeFe] Hydrogenase. Abstract. [FeFe] hydrogenase enzymes rapidly evolve H2 at a 6-Fe catalytic site termed the H-cluster, which consists of a traditional [4Fe-4S] cluster linked via a cysteine bridge to a dinuclear Fe subcluster [2Fe]H that possesses unusual biological ligands: two terminal CN- ligands, two terminal CO ligands, and azadithiolate and CO bridges, all of which are thought to be synthesized and installed by a set of Fe-S proteins denoted HydE, HydF, and HydG. With the James Swartz laboratory (Stanford University) we can generate [FeFe] hydrogenase in high yield using cell free synthesis methods, allowing for specific isotope labelling of its components as needed for definitive spectroscopic studies (1).. The radical S-adenosylmethionine (SAM) enzyme HydG lyses free L-tyrosine to produce CO and CN- for the assembly of the H-cluster. We use electron paramagnetic resonance (EPR) spectroscopy to detect and characterize HydG reaction ...
The light-driven splitting of water into its constituting elements gives access to a valuable fuel from an abundant substrate, using sunlight as the only energy source. Synthetic diiron complexes as functional models of the [FeFe] hydrogenase H(2)ase enzyme active site have moved into the centre of focus as potentially viable catalysts for the reductive side of this process, i.e. the reduction of protons to molecular hydrogen. The active site of the enzyme, as well as its mimics in an artificial system, are required to accumulate two electrons from single electron transfer events and to combine them with two protons to form hydrogen. Whereas in biology this reaction is not coupled to photosynthesis and thus proceeds in the dark, additional aspects need to be considered when designing a functional artificial system for the light-driven reduction of protons. Suitable photosensitizers have to be chosen that not only provide sufficient driving force for the reduction of the synthetic diiron ...
A series of pendant amine-containing [FeFe]-hydrogenase models, [X(CH2S-μ)2{Fe(CO)3}{Fe(CO)(P2 PhN2 Bn)}] (1H, X = CH2; 2Me, C(CH3)2; 3Et, C(CH2CH3)2; and P2 PhN2 Bn = 1,5-dibenzyl-3,7-diphenyl-1,5-diaza-3,7-diphosphacyclooctane) with different groups at the bridgehead carbon of the S-to-S linker were synthesized. The oxidations of these complexes as well as the reverse reduction reaction were studied by cyclic voltammetry and in situ IR spectroscopy. Regardless of the bridgehead steric bulk, all three complexes demonstrate intramolecular iron-mediated C(sp3)-H bond heterolytic cleavage with the assistance of the pendant amine base within the chelating diphosphine ligand in the two-electron oxidation process. X-ray crystallographic analysis shows that the doubly oxidized products, [1′H]+, [2′Me]+, and [3′Et]+, all have a rigid FeSC three-membered ring at the open apical site of the rotated iron center. The most noticeable difference in structures of the oxidized complexes is that the ...
Gabriel John Utterson and his cousin Richard Enfield reach the door of a large house on their weekly walk. Enfield tells Utterson that months ago he saw a sinister-looking man named Edward Hyde trample a young girl after accidentally bumping into her. Enfield forced Hyde to pay £100 to avoid a scandal. Hyde brought them to this door and provided a cheque signed by a reputable gentleman (later revealed to be Doctor Henry Jekyll, a friend and client of Utterson). Utterson is disturbed because Jekyll recently changed his will to make Hyde the sole beneficiary. Utterson fears that Hyde is blackmailing Jekyll. When Utterson tries to discuss Hyde with Jekyll, Jekyll turns pale and asks that Hyde be left alone. One night in October, a servant sees Hyde beat Sir Danvers Carew to death, another of Uttersons clients. The police contact Utterson, who leads officers to Hydes apartment. Hyde has vanished, but they find half of a broken cane (the other half having been left at the crime scene). Utterson ...
FeFe]-Hydrogenases (H2ases) are metalloenzymes that can catalyze the reversible reduction of protons to molecular hydrogen as part of the metabolism of certain cyanobacteria and green algae. Due to the low availability of the enzyme, synthetic complexes that mimic the natural active site in structure, function and activity are highly sought after. In this thesis, a number of [FeFe]-H2ases active site model complexes were synthesized to answer open questions of the active site and to develop unprecedented bio-inspired proton reduction catalysts.. The first part describes the synthesis and the protonation properties of a [Fe2(μ-adt)(CO)4(PMe3)2] (adt = azadithiolate) complex which contains two basic sites that are similar to those found in the enzyme active site. Unusual kinetic factors give rise to four discrete protonation states. The twofold protonated state is the first model complex that simultaneously carries a proton at the azadithiolate nitrogen and a bridging hydride at the Fe-Fe ...
The study presents a multiscale methodology consisting in a decision support tool. The selection depends on efficiency, profitability and sustainability of the process. In this model, the process structure consists in the pretreatment, the hydrolysis, the fermentation, the separation and the purification. The Acetone-Butanol-Ethanol fermentations methodology is demonstrated in this study. © 2016 Elsevier B.V. ...
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice ...
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He used the bacterium Clostridium acetobutylicum (the Weizmann organism) to produce acetone. Acetone was used in the ...
"Immobilization of Clostridium acetobutylicum DSM 792 as macroporous aggregates through cryogelation for butanol production". ...
... is produced and excreted by acetic acid bacteria, notably the genus Acetobacter and Clostridium acetobutylicum. ... However, Clostridium bacteria are less acid-tolerant than Acetobacter. Even the most acid-tolerant Clostridium strains can ... Species of anaerobic bacteria, including members of the genus Clostridium or Acetobacterium can convert sugars to acetic acid ... Sim, Jia Huey; Kamaruddin, Azlina Harun; Long, Wei Sing; Najafpour, Ghasem (2007). "Clostridium aceticum-A potential organism ...
... for biofuels production of a functional cell wall anchored minicellulosome by recombinant Clostridium acetobutylicum ATCC 824. ... 156, 828-36 (1983). Bayer, E. A., Kenig, R. & Lamed, R. Adherence of Clostridium thermocellum to cellulose. J. Bacteriol. 156, ... cellulase-containing complex in Clostridium thermocellum. J. Bacteriol. ...
See also: Clostridium acetobutylicum. Butanol is an alcohol which can be used as a fuel in most gasoline internal combustion ... It is typically a product of the fermentation of biomass by the bacterium Clostridium acetobutylicum (also known as the ...
It is typically a product of the fermentation of biomass by the bacterium Clostridium acetobutylicum (also known as the ...
One example is the production of 1-butanol in Synechococcus elongatus using enzymes from Clostridium acetobutylicum, ...
... -Clostridium Clostridium acetobutylicum Clostridium botulinum Clostridium butyricum Clostridium difficile Clostridium ... kluyveri Clostridium novyi Clostridium perfringens Clostridium phytofermentans Clostridium tetani Clostridium thermocellum ... Clostridium botulinum, Burkholderia mallei, Burkholderia pseudomallei, Clostridium perfringens, and Entamoeba histolytica) ...
For example, the solventogenic Clostridium acetobutylicum ATCC 824 has 13 altogether with 6 in the Glc family, 2 in the Fru ...
Clostridium acetobutylicum Clostridium aurantibutyricum Clostridium beijerinckii Clostridium butyricum Clostridium cadaveris ... Clostridium chauvoei Clostridium felsineum Clostridium pasteurianum Clostridium puniceum Clostridium roseum Clostridium ... In Clostridium acetobutylicum, a model solventogenic Clostridium species, a combination of low pH and high undisociated ... Some species such as Clostridium beijerinckii, Clostridium puniceum and Clostridium roseum are able to further reduce acetone ...
French, D. & Knapp, D.W. (1950). „The maltase of Clostridium acetobutylicum". J. Biol. Chem. 187: 463-471. PMID 14803428.. ...
"Metabolic engineering of Clostridium acetobutylicum for the industrial production of 1,3-propanediol from glycerol". Metab Eng ...
EPA Clostridium acetobutylicum Final Risk Assessment. *Genetic Engineering of Clostridium acetobutylicum for Enhanced ... Clostridium acetobutylicum, ATCC 824, is a commercially valuable bacterium sometimes called the "Weizmann Organism", after ... Unlike yeast, which can digest only sugar into alcohol and carbon dioxide, C. acetobutylicum and other Clostridia can digest ... One of the crucial enzymes - a fatty acyl-CoA reductase - came from Clostridium acetobutylicum. ...
Clostridium acetobutylicum), enzýmov, výroba liehu zo škrobu, mliekarenský priemysel, výroba vitamínov, antibiotik, alkaloidov ... Botulotoxín, produkt druhu Clostridium botulinum, je jedným z najsilnejších bakteriálnych toxínov vôbec. ...
Clostridium acetobutylicum. *Clostridium botulinum. *Clostridium difficile. *Clostridium perfringens. *Corynebacterium ...
Clostridium acetobutylicum. *Clostridium butyricum. *Clostridium difficile. *Clostridium perfringens. *Corynebacterium ...
Clostridium botulinum (botulism). *Clostridium acetobutylicum. *Clostridium haemolyticum. *Clostridium novyi. *Clostridium ... The Clostridia are a highly polyphyletic class of Firmicutes, including Clostridium and other similar genera. They are ... Species of the class Clostridia are often but not always Gram-positive (see Halanaerobium hydrogenoformans) and have the ... Heliobacteria and Christensenella are also members of the class Clostridia. Some of the enzymes produced by this group are used ...
C. acetobutylicum. C. aerotolerans. C. botulinum. C. butyricum. C. colicanis. C. difficile. C. formicaceticum. C. ljungdahlii. ... Clostridium (klostridij) je rod grampozitivnih bakterij iz debla Firmicutes. Bakterije iz tega rodu so obvezni anaerobi in ... V rod Clostridium spadajo tako nenevarne vrste kot tudi pomembni patogeni. Štiri vrste povzročajo pri človeku bolezni: *C. ... Vzpostavljeno iz »https://sl.wikipedia.org/w/index.php?title=Clostridium&oldid=4843481« ...
... that of manufacturing corn starch using Clostridium acetobutylicum, to produce acetone, which the United Kingdom desperately ...
The bacterium Clostridium acetobutylicum can feed on cellulose to produce butanol on an industrial scale.[30] ...
Clostridium Acetobutylicum. *Clostridium Botulinum. *Clostridium Butyricum. *Clostridium Pasteurianum. *Clostridium Tetani. * ...
... includes the genus Clostridium (e.g. the pathogens C. dificile, C. tetani, C. botulinum and the biotech C. acetobutylicum) ... Low-G+C species (later renamed Firmicutes[19]) (Clostridium, Peptococcus, Bacillus, Mycoplasma) ... the class Clostridia of mostly anaerobic sulphite-reducing saprophytic species, ...
EPA Clostridium acetobutylicum Final Risk Assessment. *Genetic Engineering of Clostridium acetobutylicum for Enhanced ... Clostridium acetobutylicum, ATCC 824, is a commercially valuable bacterium sometimes called the "Weizmann Organism", after ... Unlike yeast, which can digest only sugar into alcohol and carbon dioxide, C. acetobutylicum and other Clostridia can digest ... One of the crucial enzymes - a fatty acyl-CoA reductase - came from Clostridium acetobutylicum. ...
Clostridium acetobutylicum. Mutation(s): 1 Gene Names: CAC2707, CA_C2707. EC: 3.2.2 (PDB Primary Data), 4.2.99.18 (PDB Primary ... Find proteins for Q97FM4 (Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)) ... Crystal structure of Clostridium Acetobutylicum 8-oxoguanine DNA glycosylase in complex with 8-oxoguanosine. *DOI: 10.2210/ ... Structural characterization of Clostridium acetobutylicum 8-oxoguanine DNA glycosylase in its apo form and in complex with 8- ...
sp,Q97FT9,LON_CLOAB Lon protease OS=Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) ... Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787). ... Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) ... cellular organisms › Bacteria › Terrabacteria group › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium › ...
Clostridium acetobutylicum is an appropriate Gram-positive bacterium to study those mechanisms as genetic tools have been ... Characterization of iron reduction in Clostridium acetobutylicum mutants obtained by targeted and transposon mutagenesis. Amos ...
Thiolase of Clostridium acetobutylicum is an important enzyme involved in both, acid and solvent fermentation. Two thiolase ... have been cloned and sequenced from Clostridium acetobutylicum DSM 792, showing high homology to each other and to thiolases of ... Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. Journal of Molecular Microbiology and ...
We used HaloTag and SNAP-tag to label the strictly anaerobic organisms Clostridium acetobutylicum and Clostridium ljungdahlii. ... Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag ... Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag ... Development of Strong Anaerobic Fluorescent Reporters for Clostridium acetobutylicum and Clostridium ljungdahlii Using HaloTag ...
Clostridium acetobutylicum ATCC 824 is a commercially valuable bacterium sometimes called the Weizmann Organism after ... Similar Bacteria, Clostridium beijerinckii, Clostridia, Clostridium butyricum, Clostridium thermocellum. Clostridium ... Clostridium acetobutylicum, ATCC 824, is a commercially valuable bacterium sometimes called the "Weizmann Organism", after ... Unlike yeast, which can digest sugar only into alcohol and carbon dioxide, C. acetobutylicum and other Clostridia can digest ...
... on biohydrogen production in dark fermentation method by Clostridium acetobutylicum (PTCC 1492). Another route, i.e. [1][2] In ... capsule and exopolysaccharide was investigated in a synthetic medium in which the o Clostridium acetobutylicum starts to ... Clostridium strains with the potential of utilizing various biomass (e.g., corn cobs, cassava and rice bran) and the production ... C. acetobutylicum was the most reported in acetone-butanol-ethanol (ABE) fermentation for synthesis of biobutanol with higher ...
Clostridium acetobutylicum ATCC 824 plasmid pSOL1. Genome. Enlarge Image - Zoomable Map. Total size (bp). 192000. G + C content ...
... by Clostridium acetobutylicum YM1 from Pretreated Palm Kernel Cake in Batch Culture Fermentation ... by Clostridia. In this study, ABE production from the fermentation of PKC using Clostridium acetobutylicum YM1 in a batch ... Production of Acetone, Butanol, and Ethanol (ABE) by Clostridium acetobutylicum YM1 from Pretreated Palm Kernel Cake in Batch ... Palm kernel cake (PKC); Clostridium acetobutylicum YM1; Acetone-butanol-ethanol (ABE); Pretreatment ...
Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710/ VKM B-1787) ... Q97EW9 (RNM5_CLOAB) Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710/ VKM B-1787). Ribonuclease M5 ...
Clostridium acetobutylicum-, We can get it in the strain collection of the CINVESTAV from Mexico. ... I ask Jovita Martínez, who take care of the strain collection of CINVESTAV-Mexico, if she could give us clostridium ...
The production of biohydrogen from rice mill wastes, including rice bran and rice mill effluent by Clostridium acetobutylicum ... The production of biohydrogen from rice mill wastes, including rice bran and rice mill effluent by Clostridium acetobutylicum ...
... so far in the anaerobe Clostridium acetobutylicum, in the microaerophilic, early-branching bacterium Aquifex aeolicus, and in ...
Clostridium acetobutylicum]; and residues 15-392 are 30% similar to YQEV_BACSU.. A search of the STD-pro database reveals ...
Clostridium difficile, Clostridium botulinum, Clostridium tetani, and Clostridium perfringen. In particular, C. botulinum and C ... Clostridium acetobutylicum is a Gram-positive bacillus (1). C. acetobutylicum is most often soil dwelling, although it has been ... "Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium ... Adaptive responses to oxygen stress in obligatory anaerobes Clostridium acetobutylicum and Clostridium aminovalericum. Appl. ...
Complete genome sequence of Clostridium acetobutylicum DSM 1731, a solvent-producing strain with multireplicon genome ...
2012) New insights into the butyric acid metabolism of Clostridium acetobutylicum. Appl Microbiol Biotechnol 96(5):1325-1339. ... 2004) Transcriptional analysis of butanol stress and tolerance in Clostridium acetobutylicum. J Bacteriol 186(7):2006-2018. ... 1994) Host-plasmid interactions in recombinant strains of Clostridium acetobutylicum ATCC 824. FEMS Microbiol Lett 123(3):335- ... 1984) The relationship between sporulation and solvent production in Clostridium acetobutylicum P262. Biotechnol Lett 6(8):529- ...
Clostridium acetobutylicum, Clostridium saccharobutylicum and Clostridium beijerinckii are solventogenic Clostridia that grow ... New insights into the butyric acid metabolism of Clostridium acetobutylicum. Clostridium acetobutylicum is a Gram-positive ... By a butyric acid fermentation by Clostridium acetobutylicum resulted in clostridium acetobutylicum butyric acid number of ... Clostridia that grow by a butyric acid metabolism of Clostridium acetobutylicum has been considered an attractive platform-host ...
Genomic DNA from Clostridium acetobutylicum TypeStrain=True Application: ... Clostridium acetobutylicum McCoy et al. emend. Keis et al. (ATCC® 824D-5™) Strain Designations: Genomic DNA from Clostridium ... Clostridium acetobutylicum McCoy et al. emend. Keis et al. ATCC® 824D-5™ dried At least 5 µg in 1X TE buffer. OD260/OD280: 1.6 ... Genomic DNA from Clostridium acetobutylicum [ATCC® 824™] Biosafety Level 1 Biosafety classification is based on U.S. Public ...
Streptomycin biosynthesis - Clostridium acetobutylicum ATCC 824 [ Pathway menu , Organism menu , Pathway entry , Download KGML ...
Clostridium acetobutylicumis a natural producer of butanol, butyrate, acetone and ethanol. The pattern of metabolites reflects ... Clostridium acetobutylicum NAD(P)H Redox FdNR Ferredoxin TER Electronic supplementary material. The online version of this ... Clostridium acetobutylicum is a natural producer of butanol, butyrate, acetone and ethanol. The pattern of metabolites reflects ... Cooksley CM, Zhang Y, Wang H, Redl S, Winzer K, Minton NP (2012) Targeted mutagenesis of the Clostridium acetobutylicum acetone ...
Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum.. U Sauer, A Treuner, M Buchholz, J D ... Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum.. U Sauer, A Treuner, M Buchholz, J D ... Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum.. U Sauer, A Treuner, M Buchholz, J D ... Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum. Message Subject (Your Name) has forwarded ...
... solvents production from lignocellulosic hydrolysates by Clostridium acetobutylicum, an important industrial microorganism. ... modulation of pleiotropic regulator CcpA for glucose and xylose coutilization by solvent-producing Clostridium acetobutylicum. ... acetobutylicum. When this residue was replaced by asparagine (V302N mutation), CCR could be alleviated and a greatly improved ... acetobutylicum CcpA with alleviated repression on xylose metabolism, yielding a valuable platform host toward ABE solvents ...
The nucleotide sequence of three open reading frames in the acetone-production locus of Clostridium acetobutylicum ATCC824 has ... Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824.. ...
... acetobutylicum inhibits the production of H2by the hydrogenase and enhances the production of solvents by making available ... The effect of CO on growth and product formation in batch cultures ofClostridium acetobutylicum. ... Carbon monoxide sparged in batch fermentations ofC. acetobutylicum inhibits the production of H2 by the hydrogenase and ...
n-Butanol is a natural product of the fermentation of carbohydrates by Clostridium acetobutylicum and can be used as biofuel ... A Synthetic Biology Approach to Enhancing Metabolic Engineering of Clostridium Acetobutylicum. *Conference: Metabolic ... Formerly, C. acetobutylicum was used in the industrial Acetone-Butanol-Ethanol (ABE) fermentation to produce solvents in a ... Heap, J.T., Pennington, O.J., Cartman, S.T. and Minton, N.P. (2009) A modular system for Clostridium shuttle plasmids. J. ...
Clostridium acetobutylicum DSM 792. › Clostridium acetobutylicum str. ATCC 824. › Clostridium acetobutylicum strain ATCC 824. ... Clostridia. › Clostridiales. › Clostridiaceae. › Clostridium. › Clostridium acetobutylicum. Strains i. › ATCC 824 / DSM 792 / ... Taxonomy - Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787) Basket 0 ... Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787). ...
This dissertation presents molecular and genomic analyses in Clostridium acetobutylicum, beginning with an evaluation of Spo0A ... These proteins are novel and essentially limited to C. acetobutylicum; thus, suggesting roles specific to its unique physiology ... "Molecular and genomic analyses in Clostridium acetobutylicum." (2009) Diss., Rice University. https://hdl.handle.net/1911/61756 ...
Crystal structure of the complex of a Ferredoxin-Flavin Thioredoxin Reductase and a Thioredoxin from Clostridium acetobutylicum ... Find proteins for Q97EM8 (Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)) ... Find proteins for Q97EM7 (Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)) ... Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787). Mutation(s): 1 ...
Complementation studies were carried out with a Clostridium acetobutylicum ATCC 824 plasmid library and Escherichia coli ... "Cloning and sequencing of genes involved in glycolysis from Clostridium acetobutylicum." (1996) Masters Thesis, Rice ... Phosphofructokinase from C. acetobutylicum has high homology to the phosphofructokinases from Bacillus stearothermophilus, ... The pyruvate kinase/phosphofructokinase operon may act as a "regulatory operon" for glycolysis in C. acetobutylicum. ...
Characterization of the CipA Scaffolding Protein and In Vivo Production of a Minicellulosome in Clostridium acetobutylicum. ... Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum. J. Bacteriol. 183:4823- ... The cipA gene encoding the Clostridium acetobutylicum scaffolding protein CipA was cloned and expressed in Escherichia coli. ... Clostridium acetobutylicum is a gram-positive, sporeforming anaerobic bacterium that converts various sugars and ...
Production of Isopropanol Butanol Ethanol Mixture by Engineered Clostridium acetobutylicum. Message:. Hi! I thought youd be ... Article Title: Production of Isopropanol Butanol Ethanol Mixture by Engineered Clostridium acetobutylicum. Page URL : http:// ...
... on the membrane composition and fluidity of Clostridium acetobutylicum ATCC 824 and a butanol-tolerant mutant, SA-2, was ... of Butanol Challenge and Temperature on Lipid Composition and Membrane Fluidity of Butanol-Tolerant Clostridium acetobutylicum ... of Butanol Challenge and Temperature on Lipid Composition and Membrane Fluidity of Butanol-Tolerant Clostridium acetobutylicum ... of Butanol Challenge and Temperature on Lipid Composition and Membrane Fluidity of Butanol-Tolerant Clostridium acetobutylicum ...
As C. acetobutylicum is unable to grow on cellulosic substrates, the existence of a cellulosomal gene cluster in the genome ... Surprisingly, genetic organization of this large cluster is very similar to that of Clostridium cellulolyticum, the model of ... A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. ... A large cellulosomal gene cluster was identified in the recently sequenced genome of Clostridium acetobutylicum ATCC 824. ...
... and DNA topology in Clostridium acetobutylicum was investigated by inhibiting DNA gyrase in vivo by high concentrations of ... Transcriptional regulation of solventogenesis in Clostridium acetobutylicum.. *Peter Dürre, Michael Böhringer, Stephan Nakotte ... Cloning and expression of Clostridium acetobuty - licum genes involved in solvent production. *M. Sebald, P. Diirre, +7 authors ... DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5.. * ...
Keywords: Lyases -- Research ; Bacterial spores ; Bacillus subtilis ; Clostridium acetobutylicum ; Bioorganic chemistry ; ... However, in SPL from the bacterium Clostridium acetobutylicum (Ca), the cysteine (at position 74) and the tyrosine are located ... and Hydrogen/Deuterium Exchange Reveal Large Conformational Changes During the Catalysis of the Clostridium acetobutylicum ... and Hydrogen/Deuterium Exchange Reveal Large Conformational Changes During the Catalysis of the Clostridium acetobutylicum ...
Clostridium acetobutylicum is a model organism for both clostridial biology and solvent production. The organism is exposed to ... revealed by integrative proteomic and transcriptomic analysis of metabolite stress response in Clostridium acetobutylicum ...
The results show that C. acetobutylicum utilizes symporters and ATP-binding cassette (ABC) transporters for the uptake of ... Additionally, updates to the C. acetobutylicum genome annotation are proposed, including the identification of genes likely to ... Transcriptional analysis was performed on Clostridium acetobutylicum with the goal of identifying sugar-specific mechanisms for ... Thompson, J., Hess, S. & Pikis, A. ( 2004; ). Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+- ...
  • Clostridium acetobutylicum , ATCC 824, is a commercially valuable bacterium sometimes called the " Weizmann Organism ", after Jewish-Russian-born Chaim Weizmann . (wikipedia.org)
  • Unlike yeast , which can digest only sugar into alcohol and carbon dioxide , C. acetobutylicum and other Clostridia can digest whey , sugar , starch , cellulose and perhaps certain types of lignin , yielding butanol , propionic acid , ether , and glycerin . (wikipedia.org)
  • Two thiolase genes (thlA and thlB) have been cloned and sequenced from Clostridium acetobutylicum DSM 792, showing high homology to each other and to thiolases of PHA-synthesizing bacteria. (lancs.ac.uk)
  • the genes were derived from Clostridium acetobutylicum . (wikipedia.org)
  • Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. (lancs.ac.uk)
  • Clostridium acetobutylicum is an appropriate Gram-positive bacterium to study those mechanisms as genetic tools have been developed due to its industrial interest, allowing easy targeted and random mutagenesis. (epfl.ch)
  • Thiolase of Clostridium acetobutylicum is an important enzyme involved in both, acid and solvent fermentation. (lancs.ac.uk)
  • One of the biggest limitations in the study and engineering of anaerobic Clostridium organisms is the lack of strong fluorescent reporters capable of strong and real-time fluorescence. (asm.org)
  • Recently, we reported a new strong fluorescent reporter system based on the FAST protein as a first step in expanding the fluorescence-based reporters for Clostridium and other anaerobic microbial platforms. (asm.org)
  • I ask Jovita Martínez, who take care of the strain collection of CINVESTAV-Mexico, if she could give us clostridium acetobutylicum . (openwetware.org)
  • The availability of multiple strong fluorescent reporters is a major addition to the genetic toolkit of Clostridium and other anaerobes that will lead to better understanding of these unique organisms. (asm.org)
  • Green fluorescent protein (GFP), mCherry, and flavin-binding proteins (and their derivatives) have been used only in a few clostridia with limited success and yielded low fluorescence compared to aerobic microbial systems. (asm.org)
  • We have also identified a new strong promoter for protein expression in C. acetobutylicum , based on the phosphotransacetylase gene ( pta ) from C. ljungdahlii . (asm.org)
  • While Clostridium acetobutylicum Ogg (CacOgg) DNA glycosylase can specifically recognize and remove 8-oxoG, it displays little preference for the base opposite the lesion, which is unusual for a member of the Ogg1 family. (rcsb.org)
  • Here the exogenous genes of ferredoxin-NAD(P) + oxidoreductase (FdNR) and trans -enoyl-coenzyme reductase (TER) are introduced to three different Clostridium acetobutylicum strains to investigate the distribution of redox equivalents and butanol productivity. (springer.com)
  • While the TER functions as an NAD(P)H oxidase, butanol production was decreased in the C. acetobutylicum strains containing TER. (springer.com)
  • This strategy will also be employed in the future to create high-yield strains of C. acetobutylicum and other industrially relevant strains for production of a wide range of commodity chemicals. (aiche.org)
  • DNA array-based transcriptional analysis of asporogenous, nonsolventogenic Clostridium acetobutylicum strains SKO1 and M5. (semanticscholar.org)
  • However, the industrial application of Clostridium acetobutylicum strains for high-performance solvent yields and their extreme tolerance for butanol still require more investigation. (asm.org)
  • The goal of this study was to obtain mutant strains of Clostridium beijerinckii NRRL B-598 with improved butanol tolerance using random chemical mutagenesis, describe changes in their phenotypes compared to the wild-type strain and reveal changes in the genome that explain improved tolerance or other phenotypic changes. (frontiersin.org)
  • The two-stage, dual-path process, which relies on two different Clostridia strains ( earlier post ), also yields hydrogen as a product. (greencarcongress.com)
  • genic species is Clostridium acetobutylicum, which typically pro-duces butanol, acetone, and ethanol at the mass ratio of 6:3:1 (17, 20, 21). (jakesonline.org)
  • Clostridium acetobutylicum is a natural producer of butanol, butyrate, acetone and ethanol. (springer.com)
  • Biswas R, Zheng T, Olson DG, Lynd LR, Guss AM (2015) Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum . (springer.com)
  • Efficient cofermentation of hexose and pentose sugars is essential for ABE (Acetone, Butanol and Ethanol) solvents production from lignocellulosic hydrolysates by Clostridium acetobutylicum, an important industrial microorganism. (nih.gov)
  • Cellulase, clostridia, and ethanol. (semanticscholar.org)
  • The regA gene inhibited the expression of a C. acetobutylicum gene that allows the starch to degrade into acetone, butanol and ethanol. (readabstracts.com)
  • This study investigates the production of acetone, butanol and ethanol (ABE) in sterilized sludge of palm oil mill effluent (POME) with the addition of glucose by Clostridium acetobutylicum NCIMB 619. (elsevier.com)
  • Acetone, butanol, and ethanol (ABE) were produced from corn fiber arabinoxylan (CFAX) and CFAX sugars (glucose, xylose, galactose, and arabinose) using Clostridium acetobutylicum P260. (ingentaconnect.com)
  • Clostridia, well-known commercial biobutanol production organisms, have the capability to convert a wide variety of renewable biomass and agricultural waste materials to butanol and ethanol. (asm.org)
  • abstract = "Present investigation explores the use of pineapple peel, a food industry waste, for acetone-butanol- ethanol (ABE) production using Clostridium acetobutylicum B 527. (aalto.fi)
  • abstract = "Summary The phosphorylated Spo0A transcription factor controls the initiation of endospore formation in Clostridium acetobutylicum, but genes encoding key phosphorelay components, Spo0F and Spo0B, are missing in the genome. (aber.ac.uk)
  • The most famous species of the group of solventogenic clostridia are Clostridium acetobutylicum and Clostridium beijerinckii , both of which share a common process bottleneck - low tolerance to butanol. (frontiersin.org)
  • The recent fossil fuel crisis has spurred more research into C. acetobutylicum and the utilization of the ABE process (2) . (kenyon.edu)
  • They were then successfully exploited … and butanol by C. acetobutylicum in the utilization of agricultural and domestic wastes such as whey, wood shavings, bagasse, and rice straw has also been investigated (McNeil and Kristiansen, 1986). (jakesonline.org)
  • We then present experimental evidence that the cel operon involved in cellobiose utilization is directly regulated by CelR and σ 54 (SigL) in Clostridium acetobutylicum. (elsevier.com)
  • enhance the utilization ability of C. acetobutylicum on glucose and direct most of extra consumed glucose into acetone/butanol synthesis routes. (usda.gov)
  • It is the first utilization of this approach for the development of butanol-tolerant mutants of solventogenic clostridia, for which generally there is a lack of knowledge about butanol efflux or efflux mechanisms and their regulation. (frontiersin.org)
  • A stable solvent productivity of 3.5 g/L h was achieved by using cells of Clostridium acetobutylicum immobilized onto a packed bed of bonechar, coupled with continuous product removal by pervaporation. (membran.at)
  • Sporulation and primary sigma factor homologous genes in Clostridium acetobutylicum. (asm.org)
  • Characterization and expression of the hydrogenase-encoding gene from Clostridium acetobutylicum P262. (readabstracts.com)
  • In this study, first insights on the molecular level were gained to explore the effect of methyl viologen addition to cultures of Clostridium acetobutylicum. (omicsdi.org)
  • The 1,3- propanediol pathway from C. butyricum was introduced on a plasmid in several mutants of C. acetobutylicum altered in product formation. (ucp.pt)
  • Furthermore, to investigate the catalytic residues of CacHBD, the enzymatic activities of the wild type and of three single-amino-acid mutants were analyzed, in which the Ser, His and Asn residues that are conserved in the HBDs from C. acetobutylicum , C. butyricum and Ralstonia eutropha , as well as in the L-3-hydroxyacyl-CoA dehydrogenases from Homo sapiens and Escherichia coli , were substituted by alanines. (proteopedia.org)
  • Complete genome sequence of Clostridium acetobutylicum DSM 1731, a solvent-producing strain with multireplicon genome architecture. (genome.jp)
  • Here, we briefly describe the draft genome sequence of C. acetobutylicum GXAS18-1. (asm.org)
  • Complete genome sequence of the autotrophic acetogen Clostridium formicaceticum DSM 92 using nanopore and llumina sequencing data. (uni-ulm.de)
  • Genome sequence of the strict anaerobe Clostridium neopropionicum X4, DSM 3847T. (uni-ulm.de)
  • Conclusions: We present the full genome sequence of Clostridium beijerinckii DSM 6423, providing a complete genetic background of this strain. (deepdyve.com)
  • Bannam TL, Rood JI (1993) Clostridium perfringens- Escherichia coli shuttle vectors that carry single antibiotic resistance determinants. (springer.com)
  • Phosphofructokinase from C. acetobutylicum has high homology to the phosphofructokinases from Bacillus stearothermophilus, Bacillus macquarensis, Escherichia coli and other microbes. (rice.edu)
  • The cipA gene encoding the Clostridium acetobutylicum scaffolding protein CipA was cloned and expressed in Escherichia coli . (asm.org)
  • DNA sequencing of the hydrogenase-encoding gene (hydA) isolated by colony hybridization from Clostridium acetobutylicum P262 reveals the presence of an open reading frame of 1722 bp that codes for a protein with 574 amino acids. (readabstracts.com)
  • This gene was isolated by colony hybridization, using the Clostridium pasteurianum hydrogenase-1 gene as a probe. (microbiologyresearch.org)
  • Clostridium acetobutylicum has been considered an attractive platform-host for biorefinery due to its metabolic diversity. (jakesonline.org)
  • Bennett GN, Rudolph FB (1995) The central metabolic pathway from acetyl-CoA to butyryl-CoA in Clostridium acetobutylicum . (springer.com)
  • Subsequently, we attempted to create a high-yield strain of C. acetobutylicum by employing synthetic regulatory elements to develop and implement a system for metabolic flux redistribution. (aiche.org)
  • In the paper published in Nature Communications, the researchers show that the redox-switch of thiolase plays a role in a regulation of metabolic flux in C. acetobutylicum by using in silico modeling and simulation tools. (internationalsugarjournal.com)
  • To obtain a better ''vitamin B12-free'' biological process, we developed a metabolic engineering strategy with Clostridium acetobutylicum. (ucp.pt)
  • To reinforce a metabolic flux toward butanol production, the metabolic network of C. acetobutylicum strain was engineered with the redesigned enzyme. (eurekalert.org)
  • The combination of the discovery of 3D enzyme structure and systems metabolic engineering approaches resulted in increased n-butanol production in C. acetobutylicum , which allows the production of this important industrial chemical to be cost competitive. (eurekalert.org)
  • The pyruvate kinase/phosphofructokinase operon may act as a "regulatory operon" for glycolysis in C. acetobutylicum. (rice.edu)
  • Molecular analysis of the mannitol operon of Clostridium acetobutylicum encoding a phosphotransferase system and a putative PTS-modulated regulator. (microbiologyresearch.org)
  • Northern blot analysis reveals the translation of the C. acetobutylicum hydrogenase protein product to be from a monocistronic operon. (readabstracts.com)
  • Northern blot analysis of RNA isolated from C. acetobutylicum indicates that the C. acetobutylicum hydrogenase protein product is translated from a monocistronic operon. (microbiologyresearch.org)
  • Thus, it is fitting that C. acetobutylicum is often called the "Weizmann organism. (kenyon.edu)
  • The enzyme 3-hydroxybutyryl coenzyme A dehydrogenase, a key enzyme in solventogenesis in Clostridium acetobutylicum , was among the most up-regulated proteins during high toxin production. (jakesonline.org)
  • We have reported the 3D structure of C. acetobutylicum thiolase-a key enzyme involved in n-butanol biosynthesis, for the first time. (eurekalert.org)
  • We report here the cloning and molecular characterization of the hydrogenase-encoding gene (hydA) from C. acetobutylicum P262. (microbiologyresearch.org)
  • The transport of glucose by the solventogenic anaerobe Clostridium acetobutylicum was investigated. (napier.ac.uk)
  • Molecular modulation of pleiotropic regulator CcpA for glucose and xylose coutilization by solvent-producing Clostridium acetobutylicum. (nih.gov)
  • Sequence and arrangement of genes encoding enzymes of the acetone-production pathway of Clostridium acetobutylicum ATCC824. (nih.gov)
  • Additionally, updates to the C. acetobutylicum genome annotation are proposed, including the identification of genes likely to encode proteins involved in the metabolism of arabinose and xylose via the pentose phosphate pathway. (microbiologyresearch.org)
  • The gap gene, which was cloned and sequenced from C. acetobutylicum DSM 792, was observed to be clustering with other genes of enzymes from the glycolytic pathway. (readabstracts.com)
  • The purification and isolation of glyceraldehyde-3-phosphate dehydrogenase from Clostridium acetobutylicum was accomplished by sequential ammonium sulfate precipitation, gel filtration and anion-exchange chromatography. (readabstracts.com)
  • An NADP-dependent alcohol dehydrogenase from Clostridium acetobutylicum (CaADH) has been expressed and characterized. (nebraska.edu)
  • The relationship between solventogenesis, heat shock, and DNA topology in Clostridium acetobutylicum was investigated by inhibiting DNA gyrase in vivo by high concentrations of novobiocin. (semanticscholar.org)
  • Transcriptional regulation of solventogenesis in Clostridium acetobutylicum. (semanticscholar.org)
  • Clostridium butyricum is to our knowledge the best natural 1,3-propanediol producer from glycerol and the only microorganism identified so far to use a coenzyme B12-independent glycerol dehydratase. (ucp.pt)
  • A redox-switch of thiolase involves in butanol biosynthesis in Clostridium acetobutylicum . (eurekalert.org)
  • Clostridium acetobutylicum is a Gram-positive bacillus (1). (kenyon.edu)
  • Clostridium acetobutylicum is a Gram-positive bacillus (1).C. The butyric acid titer, BA/AA ratio, and productivity obtained in this study were the highest values reported for C. acetobutylicum, and the BA/AA ratio and productivity were also comparable to those of native butyric acid producer Clostridium tyrobutyricum. (jakesonline.org)
  • In a previous work, we demonstrated that C. acetobutylicum produced an inactive cellulosome with an apparent molecular mass of 665 kDa ( 14 ). (asm.org)
  • The solvents produced by C. acetobutylicum, predominately acetic acid, butyric acid… High cell densities of Clostridium acetobutylicum and Clostridium tyrobutyricum were obtained using the retarding agents KH 2 PO 4 (50 mM) and NaHCO 3 (200 mM). (jakesonline.org)
  • New insights into the butyric acid metabolism of Clostridium acetobutylicum. (jakesonline.org)
  • This study was designed to investigate the ability of Clostridium saccharoperbutylacetonicum N1-4 to produce butanol in a limited nutrient medium using mixtures of glucose and butyric acid as substrates. (jakesonline.org)
  • The results show that C. acetobutylicum utilizes symporters and ATP-binding cassette (ABC) transporters for the uptake of pentose sugars, while disaccharides and hexoses are primarily taken up by phosphotransferase system (PTS) transporters and a gluconate : H + (GntP) transporter. (microbiologyresearch.org)
  • Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. (lancs.ac.uk)
  • Further study will be done to produce butanol more economically on the basis of the 3D structure of C. acetobutylicum thiolase. (eurekalert.org)
  • Here, we report the development of two new strong fluorescent reporter systems for Clostridium organisms based on the HaloTag and SNAP-tag proteins, which produce strong fluorescent signals when covalently bound to fluorogenic ligands. (asm.org)
  • IMPORTANCE Up to this point, assays and methods involving fluorescent reporter proteins were unavailable or limited in Clostridium organisms and other strict anaerobes. (asm.org)
  • Green fluorescent protein (GFP), mCherry, and flavin-binding proteins (and their derivatives) have been used only in a few clostridia with limited success and yielded low fluorescence compared to aerobic microbial systems. (asm.org)
  • Analysis of the genome of Clostridium acetobutylicum has revealed three genes encoding Fur-like proteins. (omicsdi.org)
  • Direct phosphorylation of Spo0A by histidine kinases and dephosphorylation by kinase-like proteins may be a common feature of the clostridia that may represent the ancestral state before the great oxygen event some 2.4 billion years ago, after which additional phosphorelay proteins were recruited in the evolutionary lineage that led to the bacilli. (aber.ac.uk)
  • Unlike C. acetobutylicum , the expression levels of proteins involved in glycolytic and fermentative pathways in C. tyrobutyricum did not decrease even at the stationary phase. (asm.org)
  • Proteins related to energy conservation mechanisms, including Rnf complex, NfnAB, and pyruvate-phosphate dikinase that are absent in C. acetobutylicum , were identified. (asm.org)
  • Transcriptional analysis was performed on Clostridium acetobutylicum with the goal of identifying sugar-specific mechanisms for the transcriptional regulation of transport and metabolism genes. (microbiologyresearch.org)
  • Here, the transcriptional response a steady state, acidogenic (pH 6), phosphate-limited Clostridium acetobutylicum chemostat culture to different levels of n-butanol (0.25-1%) was investigated. (omicsdi.org)
  • Biochemical and Western analysis revealed that the C. acetobutylicum cellulosome comprised four major subunits, including the scaffolding protein CipA and the cellobiohydrolases Cel48A, Cel9X, and Cel9C or Cel9E. (asm.org)
  • The CipA scaffolding protein of C. acetobutylicum has never been purified, and its biochemical properties are unknown. (asm.org)
  • In addition, a gene encoding a mini-CipA scaffolding protein was constructed and expressed in C. acetobutylicum . (asm.org)
  • A) Schematic diagram of the CipA scaffolding protein from C. acetobutylicum . (asm.org)
  • Recently, we developed a strong fluorescent reporter system for Clostridium organisms based on the FAST protein. (asm.org)
  • We have also identified a new strong promoter for protein expression in C. acetobutylicum , based on the phosphotransacetylase gene ( pta ) from C. ljungdahlii . (asm.org)
  • Cloning and DNA sequencing of the genes encoding Clostridium josui scaffolding protein CipA and cellulase CelD and identification of their gene products as major components of the cellulosome. (semanticscholar.org)
  • This C. acetobutylicum hydrogenase protein product has 82% similarity and 67% identity with the C. pasteurianum hydrogenase-1 protein. (microbiologyresearch.org)
  • One of the crucial enzymes - a fatty acyl-CoA reductase - came from Clostridium acetobutylicum . (wikipedia.org)