Clinical Enzyme Tests: Analyses for a specific enzyme activity, or of the level of a specific enzyme that is used to assess health and disease risk, for early detection of disease or disease prediction, diagnosis, and change in disease status.Micrococcaceae: A family of bacteria ranging from free living and saprophytic to parasitic and pathogenic forms.Directories as Topic: Lists of persons or organizations, systematically arranged, usually in alphabetic or classed order, giving address, affiliations, etc., for individuals, and giving address, officers, functions, and similar data for organizations. (ALA Glossary of Library and Information Science, 1983)Plasticizers: Materials incorporated mechanically in plastics (usually PVC) to increase flexibility, workability or distensibility; due to the non-chemical inclusion, plasticizers leach out from the plastic and are found in body fluids and the general environment.Diethylhexyl Phthalate: An ester of phthalic acid. It appears as a light-colored, odorless liquid and is used as a plasticizer for many resins and elastomers.Equipment and Supplies: Expendable and nonexpendable equipment, supplies, apparatus, and instruments that are used in diagnostic, surgical, therapeutic, scientific, and experimental procedures.Device Approval: Process that is gone through in order for a device to receive approval by a government regulatory agency. This includes any required preclinical or clinical testing, review, submission, and evaluation of the applications and test results, and post-marketing surveillance. It is not restricted to FDA.Equipment Safety: Freedom of equipment from actual or potential hazards.Medical Device Legislation: Laws and regulations pertaining to devices used in medicine, proposed for enactment, or enacted by a legislative body.Reagent Kits, Diagnostic: Commercially prepared reagent sets, with accessory devices, containing all of the major components and literature necessary to perform one or more designated diagnostic tests or procedures. They may be for laboratory or personal use.Biological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Radioimmunoassay: Classic quantitative assay for detection of antigen-antibody reactions using a radioactively labeled substance (radioligand) either directly or indirectly to measure the binding of the unlabeled substance to a specific antibody or other receptor system. Non-immunogenic substances (e.g., haptens) can be measured if coupled to larger carrier proteins (e.g., bovine gamma-globulin or human serum albumin) capable of inducing antibody formation.Immunoassay: A technique using antibodies for identifying or quantifying a substance. Usually the substance being studied serves as antigen both in antibody production and in measurement of antibody by the test substance.Industry: Any enterprise centered on the processing, assembly, production, or marketing of a line of products, services, commodities, or merchandise, in a particular field often named after its principal product. Examples include the automobile, fishing, music, publishing, insurance, and textile industries.Science: The study of natural phenomena by observation, measurement, and experimentation.Drug Industry: That segment of commercial enterprise devoted to the design, development, and manufacture of chemical products for use in the diagnosis and treatment of disease, disability, or other dysfunction, or to improve function.Diagnosis, Computer-Assisted: Application of computer programs designed to assist the physician in solving a diagnostic problem.BooksItalyProfessional Review Organizations: Organizations representing designated geographic areas which have contracts under the PRO program to review the medical necessity, appropriateness, quality, and cost-effectiveness of care received by Medicare beneficiaries. Peer Review Improvement Act, PL 97-248, 1982.Sensitivity and Specificity: Binary classification measures to assess test results. Sensitivity or recall rate is the proportion of true positives. Specificity is the probability of correctly determining the absence of a condition. (From Last, Dictionary of Epidemiology, 2d ed)Expert Systems: Computer programs based on knowledge developed from consultation with experts on a problem, and the processing and/or formalizing of this knowledge using these programs in such a manner that the problems may be solved.Infectious Disease Transmission, Vertical: The transmission of infectious disease or pathogens from one generation to another. It includes transmission in utero or intrapartum by exposure to blood and secretions, and postpartum exposure via breastfeeding.Pregnancy Complications, Infectious: The co-occurrence of pregnancy and an INFECTION. The infection may precede or follow FERTILIZATION.HIV Infections: Includes the spectrum of human immunodeficiency virus infections that range from asymptomatic seropositivity, thru AIDS-related complex (ARC), to acquired immunodeficiency syndrome (AIDS).Birth Rate: The number of births in a given population per year or other unit of time.HIV-1: The type species of LENTIVIRUS and the etiologic agent of AIDS. It is characterized by its cytopathic effect and affinity for the T4-lymphocyte.Technical ReportPregnancy: The status during which female mammals carry their developing young (EMBRYOS or FETUSES) in utero before birth, beginning from FERTILIZATION to BIRTH.Tropical Medicine: The branch of medicine concerned with diseases, mainly of parasitic origin, common in tropical and subtropical regions.Glucosephosphate Dehydrogenase Deficiency: A disease-producing enzyme deficiency subject to many variants, some of which cause a deficiency of GLUCOSE-6-PHOSPHATE DEHYDROGENASE activity in erythrocytes, leading to hemolytic anemia.Primaquine: An aminoquinoline that is given by mouth to produce a radical cure and prevent relapse of vivax and ovale malarias following treatment with a blood schizontocide. It has also been used to prevent transmission of falciparum malaria by those returning to areas where there is a potential for re-introduction of malaria. Adverse effects include anemias and GI disturbances. (From Martindale, The Extra Pharmacopeia, 30th ed, p404)Glucosephosphate DehydrogenasePeriodicals as Topic: A publication issued at stated, more or less regular, intervals.Favism: Hemolytic anemia due to the ingestion of fava beans or after inhalation of pollen from the Vicia fava plant by persons with glucose-6-phosphate dehydrogenase deficient erythrocytes.Publishing: "The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.

Various forms of chemically induced liver injury and their detection by diagnostic procedures. (1/534)

A large number of chemical agents, administered for therapeutic or diagnostic purposes, can produce various types of hepatic injury by several mechanisms. Some agents are intrinsically hepatotoxic, and others produce hepatic injury only in the rare, uniquely susceptible individual. Idiosyncrasy of the host is the mechanism for most types of drug-induced hepatic injury. It may reflect allergy to the drug or a metabolic aberation of the host permitting the accumulation of hepatotoxic metabolites. The syndromes of hepatic disease produced by drugs have been classified hepatocellular, hepatocanalicular, mixed and canalicular. Measurement of serum enzyme activities has provided a powerful tool for studies of hepatotoxicity. Their measurement requires awareness of relative specificity, knowledge of the mechanisms involved, and knowledge of the relationship between known hepatotoxic states and elevated enzyme activities.  (+info)

Comparison of a parasite lactate dehydrogenase-based immunochromatographic antigen detection assay (OptiMAL) with microscopy for the detection of malaria parasites in human blood samples. (2/534)

Microscopic examination of blood smears remains the gold standard for malaria diagnosis, but is labor-intensive and requires skilled operators. Rapid dipstick technology provides a potential alternative. A study was conducted in The Gambia to compare the performance of OptiMAL, an immunochromatographic antigen detection assay for the diagnosis of malaria using parasite lactate dehydrogenase, against standard microscopy in patients with suspected malaria. For initial diagnosis of Plasmodium falciparum, irrespective of stage, this assay had a sensitivity of 91.3%, a specificity of 92%, a positive predictive value of 87.2%, and a negative predictive value of 94.7%. The sensitivity of the test decreased markedly at parasitemias < 0.01%. This assay can be used for the diagnosis of malaria in areas where microscopy is not available and for urgent malaria diagnosis at night and at weekends, when routine laboratories are closed and when relatively inexperienced microscopists may be on duty.  (+info)

Underestimation of acute pancreatitis: patients with only a small increase in amylase/lipase levels can also have or develop severe acute pancreatitis. (3/534)

BACKGROUND: In most treatment studies on acute pancreatitis, pancreatologists base their diagnosis on amylase/lipase levels more than three times above the upper limit of normal (>3n) and thus exclude patients with smaller enzyme level increases. The recommendations derived from the results of treatment studies do not take into account such patients. Non-pancreatologists frequently believe that only patients with high enzyme levels have a serious prognosis. AIMS: To question the assumption that high enzyme levels indicate severe, and conversely low enzyme levels indicate mild, acute pancreatitis. PATIENTS/METHODS: This retrospective study includes 284 consecutive patients with a first attack of acute pancreatitis. The cause was biliary in 114 (40%) patients, alcoholism in 83 (29%), other in 21 (7%), and unknown in 66 (23%). Patients were divided into two groups according to their serum enzyme levels (amylase: 3n, n = 196; lipase: 3n, n = 233). Renal impairment, indication for dialysis and artificial ventilation, development of pseudocysts, necessity for surgery, and mortality were taken as parameters of severity. RESULTS: The incidence of severity was the same for both the 3n groups. CONCLUSIONS: The severity of acute pancreatitis is independent of the elevation in serum amylase/lipase level (3n) on admission. Patients with only a slight increase can also have or develop severe acute pancreatitis. Patients with +info)

Myocardial infarction in a pre-menopausal woman with angiographically normal coronary arteries. (4/534)

A young pre-menopausal non-drug-addict woman without risk factors for coronary artery disease suffered from a non-Q-wave acute myocardial infarction. She presented with epigastric pain and vomiting. Diagnosis of acute myocardial infarction was not suspected at first because of her young age and lack of risk factors. She was treated for gastritis but worsening of epigastric pain and its radiation to chest warranted the diagnosis of acute myocardial infarction, which was confirmed by serial serum cardiac enzymes. Subsequent coronary angiogram revealed normal coronary arteries.  (+info)

In situ zymographic localisation of type II collagen degrading activity in osteoarthritic human articular cartilage. (5/534)

OBJECTIVES: Chondrocytic matrix metalloproteinases (MMPs) are believed to be important in osteoarthritic cartilage degradation. The cartilage lesion of osteoarthritis (OA) is focal and often progressive. During its development chondrocytes differentially up and down regulate production of mRNA for individual MMPs. This observation has potential implications for understanding the disease processes that lead to progressive cartilage loss in OA and designing appropriate targeted treatment. The complex regulation of MMP mediated effects means there is a pressing need to establish whether visualisation of MMP mRNA or protein equates to enzyme activity. The technique of in situ zymography (ISZ) offers a way of examining diseased human tissue for in vivo production of an excess of degrading enzyme over inhibitor. The primary objective of this study was to assess, and if positive follow, collagen II degrading activity in cartilage during development of the OA lesion. A secondary objective was to assess whether there was any correlation between sites of collagen II degrading activity and expression of the collagenase (MMP-13), recently implicated in type II collagen degredation in this lesion. METHODS: Biopsied human normal and osteoarthritic cartilage, showing various degrees of damage, was examined by in situ zymography, with and without enzyme inhibitors, to establish sites of type II collagenase activity. Paired samples were probed for MMP-13 mRNA using 35S-labelled oligonucleotide probes. Comparative analyses were performed. RESULTS: In situ zymography showed collagen II degrading activity over chondrocytes only in osteoarthritic cartilage. Distribution and amount varied with the extent of cartilage damage and position of chondrocytes, being greatest in deep cartilage and in cartilage lesions where fissuring was occurring. The enzyme causing the degradation behaved as a matrix metalloproteinase. MMP-13 mRNA expression codistributed with the type II collagenase activity. CONCLUSION: In OA, chondrocytes can degrade type II collagen. The type II collagen degrading activity varies in site and amount as the cartilage lesion progresses and throughout codistributes with MMP-13 mRNA expression.  (+info)

Ruling out acute myocardial infarction early with two serial creatine kinase-MBmass determinations. (6/534)

AIMS: We studied the diagnostic value for acute myocardial infarction of serial creatine kinase-MBmass measurements on admission and at 7 h after the onset of symptoms. METHODS AND RESULTS: Patients presenting to our chest pain unit with symptoms of <5-h duration were eligible. Patients were kept under observation at least until 12 h after onset of symptoms. Blood samples were drawn on admission and 7 and 10 h after onset of symptoms. Creatine kinase-MBmass>7.0 microg x 1(-1) (upper reference limit for acute myocardial infarction), or an increase >2.0 microg x 1(-1) (reference change value) between admission and at 7 h was considered abnormal. Of a total of 470 patients, 248 patients had acute myocardial infarction: 100 out of the 248 patients had a single creatine kinase-MBmass>7.0 microg x 1(-1) on admission (sensitivity 40%, 95% CI:34-46%), 234/248 patients at 7 h (sensitivity 94%, 95% CI:91-97%), and 240/248 at 10 h (sensitivity 97%, 95% CI:94-99%). At 7 h, 246/248 patients had either a single creatine kinase-MB >7.0 microg x 1(-1) or a significant increase between admission and 7 h (sensitivity 99%, 95% CI:98-100%). Of 222 patients without acute myocardial infarction, 214 had a normal serial creatine kinase-MBmass (specificity 96%, 95% CI:93-98%). CONCLUSION: In patients with symptoms of <5-h duration, acute myocardial infarction can be ruled out using serial creatine kinase-MBmass taken on admission and at 7 h.  (+info)

Examination of synovial fluid and serum hyaluronidase activity as a joint marker in rheumatoid arthritis and osteoarthritis patients (by zymography). (7/534)

OBJECTIVE: Hyaluronic acid (HA) is an important joint marker and the substrate for hyaluronidase (HAase). Synovial fluid (SF) and serum HAase were measured to investigate the potential use of HAase as a joint marker in rheumatoid arthritis (RA) and osteoarthritis (OA) patients. METHODS: The subjects were 39 patients with RA and 42 patients with OA. HAase activity was measured by zymography and its relation with various parameters examined statistically. RESULTS: In RA SF a positive correlation (r = 0.458, p = 0.0186) was found between SF HAase activity and the concentration of serum C reactive protein. A positive correlation (r = 0.45, p = 0.024) was also found between SF HAase activity and platelet count in the RA group. Serum HAase activity in the RA group was significantly higher than in the OA group (p < 0.0001) and normal controls (p < 0.0001). CONCLUSION: The results suggest that SF HAase activity could be used as a marker of synovial inflammation.  (+info)

Biochemical evidence of thiamin deficiency in young Ghanian children. (8/534)

Detailed biochemical studies for nutritional status were carried out on 146 Ghanaian children ages 6 months to 6 years over a 2-year period. Study children comprised three main groups: severe protein-calorie malnutrition; mild to moderate protein-calorie malnutrition and apparently healthy children. Erythrocyte transketolase activity and the percentage of erythrocyte transketolase pyrophosphate effect were also determined. In the first year of the study elevated percentage of transketolase pyrophosphate effect indicative of thiamin deficiency was found in all three of the above-mentioned groups, with the most widespread deficiency in the normal groups. In year 2, repeat studies of the severely malnourished group after 2 weeks of nutritional therapy with the administration of vitamin capsules, which included thiamin, resulted in the normalization of transketolase pyrophosphate effect. Apoenzyme activity was comparable in all groups studied. There were no obvious clinical signs of thiamin deficiency, although sensory testing was not performed. A relatively large number of children with high percentage of transketolase pyrosphosphate effect also had serum folic acid deficiency. This evidence of widespread biochemical thiamin deficiency is indicative of an at-risk population among young children for clinical thiamin deficiency. Further studies are needed to identify whether the problem is inadequate thiamin intake, destruction of thiamin by thiaminases or food preparation methods, or malabsorption of thiamin.  (+info)

  • Clinical Trials a. (
  • The triple-combination drug called Kaftrio (TriKafta in the US) demonstrated significant improvements in clinical trials of people with the most common form of cystic fibrosis. (
  • In addition to collecting routine health measurements, researchers will study detailed imaging, functional, biological and quality of life measurements that could not be collected in clinical trials. (
  • Clinical trials occur under very controlled conditions and exclude many sick people with cystic fibrosis," he said. (
  • In clinical trials, doses above 10 mg daily showed an increased blood pressure response but a large increase in the rate of peripheral edema and other vasodilatory adverse events (see ADVERSE REACTIONS ). (
  • This drug is about to start phase 3 clinical trials. (
  • In light of the lack of toxicity and lack of immunogenicity of the antibody observed in the present study and the role for IL-15 in the pathogenesis of autoimmune diseases, clinical trials should be performed using the humanized version of Mikβ1 in groups of patients with human T cell lymphotropic virus I-associated myelopathy/tropical spastic paraparesis, rheumatoid arthritis, multiple sclerosis and refractory celiac disease. (
  • In the present study we translate these observations concerning IL-15 blockade into clinical trials using the monoclonal antibody Mikβ1. (
  • It has been tested in Europe in adults with brain cancer, squamous cell carcinoma and melanoma and shown in those trials to be safe. (
  • As with all clinical trials of new anticancer therapies in patients, many factors can influence the risk for severe side effects and anticancer activity. (
  • A novel test that detects enzymes that are dysfunctional in patients with Alzheimer's disease-and that are found both in the brain and in skin cells-is about to undergo large clinical trials. (
  • In preliminary clinical trials, the skin test could accurately predict which patients had Alzheimer's disease. (
  • Alkon says that the skin test can distinguish between other types of neurological diseases, such as Parkinson's, Huntington's, and Lewy body disease-a finding that he aims to confirm in larger clinical trials. (
  • Ocular delivery of AAV2 in rare orphan blindness began showing early efficacy in several clinical trials in 2008 ( here , here , here ). (
  • In placebo-controlled clinical trials, discontinuation due to side effects occurred in 1.8% of patients in the amlodipine and valsartan tablet-treated patients and 2.1% in the placebo-treated group. (
  • The adverse experiences that occurred in clinical trials (≥2% of patients) at a higher incidence than placebo included peripheral edema, nasopharyngitis, upper respiratory tract infection, and dizziness. (
  • This study used the Sequential Parallel Comparison Design (SPCD), which is geared toward reducing the impact of high placebo responses in clinical trials. (
  • 4 MIF is the only species-specific antibody test available that can measure isotype-specific antibody titers to all Chlamydia species simultaneously. (
  • Immunoassays are tests based on the very specific binding that occurs between an immunoglobulin (called an antibody ) and the substance that it specifically recognizes (the foreign molecule, called an antigen ). (
  • Immunoassays can be used to test for the presence of a specific antibody or a specific antigen in blood or other fluids. (
  • When immunoassays are used to test for the presence of an antibody in a blood or fluid sample, the test contains the specific antigen as part of the detection system. (
  • If the antibody being tested for is present in the sample, it will react with or bind to the antigen in the test system and will be detected as positive. (
  • It is based on the principle that antibodies will bind to very specific antigens to form antigen-antibody complexes, and enzyme-linked antigens or antibodies can be used to detect and measure these complexes. (
  • A second antibody (against human antibodies) that is labeled with an enzyme is then added. (
  • If the enzyme-linked antibody binds to human antibodies, the enzyme will create a detectable change that indicates the presence and amount of the antibody in the patient sample. (
  • Measurement of 14-3-3η complements RF and CCP antibody tests and may improve diagnostic sensitivity. (
  • Serological tests are helpful in detecting celiac dis- by the indirect immuno-flourescent assay (IFA) tech- ease in individuals with nongastrointestinal symp- nique is technically challenging and is being replaced toms, and high-risk groups who may or may not by the tissue transglutaminase antibody test. (
  • The ser- fluorescence analysis of the endomysial antibody ological tests utilized in current clinical practice test. (
  • This test adds radiolabeled anti-hCG antibody to nonlabeled hCG of the blood sample. (
  • No test or test panel can currently perform all these tasks because increases in specificity usually lead to reciprocal decreases in sensitivity, and because some of the clinical features of SLE are not antibody mediated. (
  • Therefore, the information obtained from any test will reflect the types of antibody detected, the prevalence of the disease in the population being tested, and the question being asked of the test. (
  • Aetna considers measurement of autoantibodies against 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase (anti-HMGCR antibody testing) medically necessary to diagnose statin-associated autoimmune myopathy in statin-exposed persons with myalgias and elevated creatnine kinase (greater than 10 times the upper limit of normal) that persist for two months (or less if symptoms progress) after discontinuation of statins. (
  • For myositis antibody panel, see CPB 0866 - Rheumatic Diseases: Selected Tests . (
  • A mix of SEO tips, good marketing strategy, and tested usability features, here's the eight must-haves for your medical practice website. (
  • Widespread incorporation of these guidelines into clinical practice has resulted in a dramatic decrease in the rate of perinatal HIV transmission and has contributed to more than a 75% decrease in reported cases of pediatric acquired immunodeficiency syndrome (AIDS) since 1992. (
  • Rooted in both medical genetics and clinical medicine, the book fills the gap between state-of-the-art technology and evidence-based practice, providing an educational opportunity for users to advance patient care by transferring NGS to the needs of real-world patients. (
  • Urine analysis Communication in clinical practice will be a topic covered both theoretically as an important aspect of clinical skill, and as a component of the practical sessions. (
  • Toward a clinical practice guide in pharmacogenomics testing for functional polymorphisms of drug-metabolizing enzymes. (
  • The development of clinical practice recommendations or guidelines for the clinical use of biomarkers is an issue of great importance with regard to adverse drug reactions. (
  • In this perspective article, we compare the relative importance of 29 gene/drug pairs in the Spanish study with that of the same pairs in the American Society for Clinical Pharmacology and Therapeutics study, and we provide suggestions and areas of focus to develop a guide for clinical practice in pharmacogenomics testing. (
  • For non-ST-segment elevation acute coronary syndrome (NSTEACS) there is a gap between the use of class I guideline recommended therapies and clinical practice. (
  • Time-tested clinical assumptions about appropriate dietary therapies during pregnancy tend to work as well in practice as do evidence-based therapies. (
  • In these assays, the intensity of the test line is inversely proportional to the analyte concentration. (
  • The CLSI ® guideline was written to ensure the use of a basic level of water purity so that clinical chemistry assays could be run safely. (
  • The purpose of enzymology assays is to measure the presence and activity of various enzymes involved in critical biochemical processes. (
  • In general, the requirements that apply to water used in the genomics field also apply to water used by clinical laboratories performing these types of assays. (
  • This was attributed to rigorous use of immunoassays specially Radioimmunoassays (RIA), and Enzyme Linked Assays (EIA) for determination of the levels of the hormone from the biological samples. (
  • Real-time Polymerase Chain Reaction (PCR) is the preferred method of diagnostic testing for acute C. pneumoniae infection, assuming the availability of an appropriate specimen type. (
  • The test incorporates multiplex Polymerase Chain Reaction (PCR) and multiplex Allele Specific Primer Extension (ASPE) on the Luminex platforms. (
  • In the first comprehensive analysis of clinical trial enrollment among older adults with blood cancers, researchers from the U.S. Food and Drug Administration found significant gaps in participation among those aged 75 and older when considered against the incidence of these malignancies in this age group, according to research being presented today during the 59th American Society of Hematology Annual Meeting and Exposition in Atlanta. (
  • During the hematology rotation the residents go once aweek to the hematology clinic during which time they are involved in clinical diagnostic hematology under the guidance of a clinical hematologist, and they also learn to do bone marrow aspirates. (
  • The visionary investment by the US National Institute of Dental and Craniofacial Research, the discovery of salivary biomarkers, and the ongoing development of salivary diagnostic technologies have addressed its diagnostic value for clinical applications. (
  • Currently, there are multiple commercially available systems for the detection of C. pneumoniae infection, including several Food and Drug Administration (FDA)-cleared tests. (
  • 2 State and local departments of public health may also offer these diagnostic tests for the detection of C. pneumoniae . (
  • Tests are performed to monitor levels of endocrine hormones (such as Follicle stimulating hormone (FSH), Luteinizing Hormone (LH), estradiol, insulin and others) from biological samples These tests aid in detection of deviation in the hormone production i.e. either hypersecretion (excess secretion) or hyposecretion (less secretion) that results in certain endocrine disorders such as infertility, menopause, thyroid dysfunction and others. (
  • Alkon's test is just one in a number of new approaches to early detection, including measuring molecules linked to the disease in blood and cerebrospinal fluid, as well as new brain-imaging markers that can detect signs of Alzheimer's, such as buildup of the protein beta-amyloid-a hallmark of the disease. (
  • Full implementation of recommendations for universal, routine prenatal HIV testing and evaluation of missed prevention opportunities will be critical to further decrease the incidence of pediatric HIV infection in the United States. (
  • These tests analyze variations in the sequence, structure, or expression of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in order to diagnose disease or medical conditions, infection with an identifiable pathogen, or determine genetic carrier status. (
  • infection based on laboratory testing using a method listed in the confirmed criteria. (
  • Finally after being unable to go to work for a few weeks because of bladder infection symptoms(but no bladder infection)the elevated enzymes were found. (
  • To be able to arrest the spread of infection during epidemics, various environmental as well as clinical samples are tested, boosting the demand for E. coli testing globally. (
  • The new The prevalence of HTLV-1 infection in a remote Northern Territory community was high: 30 of 74 adults tested were HTLV-1-positive, and nine had clinical syndromes potentially attributable to HTLV-1 infection. (
  • The implications HTLV-1 infection may be more prevalent among Indigenous Australians and be associated with a greater burden of clinical disease than is currently appreciated. (
  • I just found out about the enzyme elevation after going to several doctors over a period of five years with complaints of extreme fatigue,nasuea,body aches and pains and inability to get out of bed and go to work for short periods of time. (
  • Typically, the degree of elevation of PHE is correlated with the severity of the enzyme block. (
  • All three enzymes have different times for initial elevation, peak and resolution. (
  • The disorder is characterized by bruising, fatigue, anemia, low blood platelet count and enlargement of the liver and spleen, and is caused by a hereditary deficiency of the enzyme glucocerebrosidase (also known as glucosylceramidase), which acts on glucocerebroside. (
  • From critical therapeutic and disease research and prenatal screening, to environmental testing and industrial monitoring, we are actively engaged in improving health and advancing quality and longevity of life all around the world. (
  • EMIT therapeutic drug monitoring tests provide accurate information about the concentration of such drugs such as immunosuppressant drugs and some antibiotics. (
  • Researchers at the Blanchette Rockefeller Neurosciences Institute (BRNI), in Morgantown, WV, who developed the diagnostic have also garnered approval from the Food and Drug Administration to test in humans an experimental drug that activates the enzymes-a mechanism that represents a new therapeutic approach to Alzheimer's. (
  • other tests for infectious diseases. (
  • Therefore, this paper aims to investigate and identify the available IoT applications and their clinical utility for common diseases in elderly people. (
  • E. coli testing has thus become crucial to curb the spread of diseases such as diarrhea and hemolytic uremic syndrome (HUS). (
  • Users will find valuable tactics they can use to build a systematic framework for understanding the role of NGS testing in both common and rare diseases and conditions, from prenatal care, like chromosomal abnormalities, up to advanced age problems like dementia. (
  • Diagnostic dilemmas in which different diseases may present with similar clinical signs and laboratory data are also included. (
  • Sandwich immunoassays are the foundation for POC tests for infectious diseases that detect microbial products in clinical samples, e.g., the group A streptococcal cell wall carbohydrate ( Table 1 ). (
  • This book will be a succinct reference for the very important interface between infectious diseases and medical entomology.Jerome Goddard summarizes the latest thinking about the biological, entomological, and clinical aspects of the major vector-borne diseases around the world. (
  • The $600,000 grant from FDA is part of a program encouraging clinical development of "orphan drugs" as new treatments for rare diseases or conditions. (
  • A number of these tests have been successful in separating Alzheimer's patients from normal subjects, but most of them have not as yet compared those with AD to those with other neurological diseases, such as ALS," says Gilman. (
  • The rise in the prevalence of water and foodborne diseases is one of the key drivers for the growth of the E. coli testing market. (
  • Diseases tested for include Phosphofructokinase deficiency (PFK), McArdle's disease, Tarui's disease, Phosphoglycerate kinase deficiency (PGK), Phosphoglycerate mutase deficiency (PGAM), Lactate Dehydrogenase deficiency (LDH), Glycogen, Phosphorylase A+ total deficiency (Ph), Phosphorylase B kinase deficiency (PhK), Carnitine Palmitoyltransferase 2 deficiency (CPT2) and Myoadenylate Deaminase deficiency (MAD). (
  • The home pregnancy test that uses the lateral flow format provided clear evidence of the value of the format for at-home use of antigen testing. (
  • In an antigen-capture sandwich format, the intensity of the signal at the test line is proportional to the concentration of the analyte. (
  • As a leading provider of immunoassays to the diagnostic industry, Fujirebio, as a global team, is joining the fight to combat the COVID-19 pandemic with the goal of providing the first fully automated antigen test. (
  • As the first company to develop a fully automated SARS-CoV-2 antigen test, we expect this innovation to have a positive impact on result turnaround times and laboratory throughput of COVID-19 testing. (
  • Scientists aim to commercialize a test that detects an inflammatory marker in skin. (
  • An ideal test would be specific (detects only those with disease), sensitive (detects all those with disease), have a high positive predictive value (PPV)-where most positives have disease, and a high negative predictive value (NPV)-where most negatives do not have disease. (
  • The test, using muscle tissue, detects specific enzymes related to metabolic function. (
  • Key players in the market are Clinical- Becton, Dickinson and Company, Thermo Fisher Scientific, Inc., F. Hoffmann-La Roche Ltd. The threat of new entrants is expected to be moderate because there are various favorable conditions as well as those that go against new entrants. (
  • The main purpose of this study is to evaluate the potential effects of a single dose of sirukumab on the pharmacokinetics (what the body does to a drug) of study agents that are specific for cytochrome P450 (CYP) enzymes (CYP3A4, CYP2C9, CYP2C19, and CYP1A2) in patients with active rheumatoid arthritis (RA). (
  • This form enables patients to ask specific questions about lab tests. (
  • UC San Francisco physician-scientists have developed a test that can predict how patients with juvenile myelomonocytic leukemia (JMML) will respond to treatment, and may also be able to identify patients who are likely to recover spontaneously with little to no treatment. (
  • Among younger patients newly diagnosed with chronic lymphocytic leukemia (CLL), treatment with a combination of chemotherapy and a molecularly targeted drug significantly improves response over what is typically seen with chemotherapy alone, according to an investigator-initiated multi-center phase II clinical trial. (
  • describe the tests you would order for patients exposed to arsenic. (
  • All six patients whose profound deficiency was confirmed had enzyme activities below 15% on neonatal screening. (
  • Patients with the lowest enzyme activities usually present with the severest symptoms. (
  • To understand the epidemiology of these iso- lates, we studied 203 PDRAB isolates, taken from January 1999 to April 2000: 199 from 73 hospitalized patients treated at different clinical settings in the hospital and 4 from environmental sites in an intensive- care unit. (
  • Organisms were categorized as susceptible, intermediate, or ered from three patients admitted to three general wards in resistant to the antimicrobial agents tested on the basis of January-February 1999. (
  • The Phase I trial at Cincinnati Children's Hospital Medical Center currently focuses on testing the safety of the agent HSV1716 in patients. (
  • Physicians diagnose the disease in patients with a combination of cognitive tests to assess mental function, a neurological exam, and brain scans to rule out other problems, such as stroke or brain tumors. (
  • A method to accurately diagnose patients in the early stage of the disease could also aid in development of these therapies by allowing physicians to test experimental drugs in only those who need it. (
  • Preliminary results from a study of 600 patients are promising: the test correctly diagnosed 36 out of 37 patients confirmed on autopsy to have the disease, as well as 5 patients confirmed on autopsy not to have it. (
  • Also, in that same month in 2011, MSK investigators published data on their clinical trial of ten patients with ALL in Blood . (
  • Routine testing is currently recommended only in patients who are most likely to be infected with HCV. (
  • Computer- tive (85-98%) and specific (97-100%), the test has ized tomography techniques may be useful in diag- several limitations including false-negative results in nosing the complications of celiac sprue such as 23% of patients with celiac disease who have selec- development of lymphoma, malignancy, hyposplen- tive IgA deficiency. (
  • In gation and may become an accepted diagnostic test a study of 101 patients with untreated celiac disease in the future. (
  • In this study, we analyzed 78 tagging single-nucleotide polymorphisms (SNPs) in ABCB1 , ABCC1 and ALDH1A1 that were selected to comprehensively capture germline genetic variations in these key pharmacokinetic genes for cyclophosphamide and doxorubicin, among 882 breast cancer patients enrolled in a cooperative group clinical trial SWOG S0221. (
  • Clinical tests of Dr. Katz's patients show a 98% reduction in sulfur-producing bacteria. (
  • Because of the risk of vision loss, Vigabatrin should be withdrawn from patients with refractory complex partial seizures who fail to show substantial clinical benefit within 3 months of initiation and within 2-4 weeks of initiation for patients with infantile spasms, or sooner if treatment failure becomes obvious. (
  • The University of California, San Diego Medical Center is the first hospital in California to enroll patients in a multi-center clinical trial, sponsored by Angioblast Systems Inc., to examine the safety and feasibility of administering adult stems cells to treat congestive heart failure. (
  • Sixty patients will be recruited for this clinical trial through UC San Diego Medical Center and hospitals nationwide. (
  • This Phase 2 clinical trial will evaluate whether or not adult stems cells can be targeted to the heart muscle to repair and help improve cardiac function in patients with severe heart disease," said Anthony DeMaria, M.D., director of the Sulpizio Family Cardiovascular Center at UC San Diego Medical Center. (
  • Ron Ferstadt, 71, of Los Angeles is one of the first patients to enroll in the clinical trial. (
  • The central hypothesis is that women participating in the treatment program will have decreased rates of hazardous drinking and improved clinical and behavioral health outcomes that are associated with hazardous drinking. (
  • This is a necessary first step toward building a clinical tool that will help assess risk of adverse outcomes before undergoing chemotherapy. (
  • Glucocerebrosidase (leukocyte) is measured by fluorometric enzyme assay using 4-methylumbelliferyl-beta-D-glucosida as substrate, with/without glucocerebrosidase-specific inhibitor. (
  • The sensitivity of the experimental rapid test was 0.98 and the specificity was 0.98 using specimens preserved in heparin, and 0.98 and 0.97, respectively, for specimens preserved in EDTA. (
  • There are two significant reasons for the importance of water in the clinical setting: the need to comply with norms or guidelines (e.g., those of the Clinical and Laboratory Standards Institute ® - CLSI ® ) 1 and the sensitivity of the chemistries themselves to water quality. (
  • CT impact on the sensitivity and specificity of this test enterography techniques are currently under investi- include laboratory variations and disease severity. (
  • CLEIA technology and automated testing provides increased sensitivity, reproducibility and throughput over single-use point-of-care tests. (
  • During a blood test, you may have slight pain or bruising at the spot where the needle was put in, but most symptoms go away quickly. (
  • In addition, activating the enzymes in animal models of the disease alleviates symptoms, preventing memory loss as well as the abnormal aggregations of protein that build up in the brain. (
  • Possible treatments for these disorders may include L-dopa (for dopa-responsive dystonia) with carbidopa, serotonin, and folinic acid, which may provide significant improvement in clinical symptoms if treated early. (
  • A total of 154 samples from individuals with febrile illness having dengue fever (DF)-like symptoms were tested. (
  • Researchers from the University of Colorado Skaggs School of Pharmacy and Pharmaceutical Sciences, selected to estimate the cost-effectiveness of the newly approved CAR-T therapies, have found the clinical benefit may justify the expensive price. (
  • We badly need diagnostic tests for people with mild cognitive impairment and other people at risk for Alzheimer's disease, because there may be in the future disease-modifying therapies that could slow onset or progression of the disease," says Sid Gilman , director of the Michigan Alzheimer's Disease Research Center, at the University of Michigan. (
  • Living medicines based on cell and gene therapy have moved into mainstream on the promise of compelling clinical data, and there are literally dozens of companies, big and small alike, advancing the next generation of this genre of therapies. (
  • To better understand the clinical implications of these results, we performed a retrospective review of all MGMT testing at our laboratory over a 5-year period, and correlated test results with outcome and specimen-quality data. (
  • Finally, our analyses of specimen-quality data suggest that a combination of technical factors (eg, small samples) and tumor biology may explain inconsistent MGMT results on replicate methylation-specific PCR testing. (
  • Via the standard angiographic techniques, coronary angiographies were done and the presence and severity of CAD were determined through the clinical vessel score and the Gensini score. (
  • The association between the G20210A polymorphism and the severity of CAD with respect to the vessel score also showed no significant linear trend of higher numbers of diseased vessels (P= 0.765 for the Mantel-Haenszel test of linear trend) in the AA+GA genotype as compared with the GG genotype. (
  • The presence or absence of the skin lesions is not helpful, however, in predicting clinical severity in Hunter syndrome. (
  • Although Hunter syndrome is associated with a broad spectrum of clinical severity, two main forms can be recognized - severe and mild/attenuated. (
  • Spurred by the same and other growth drivers, the global E. coli testing market will exhibit a CAGR of 8.0% between 2017 and 2025. (
  • One or both types of tests may be used to help monitor amylase levels in people who are being treated for pancreatic or other disorders. (
  • The global endocrine testing market is surging due to the rise in geriatric population that is highly susceptible to endocrine system disorders followed by continuous technological advances along with increasing healthcare awareness. (
  • Disorders of biopterin metabolism include the enzymes guanosine triphosphate cyclohydrolase (GTPCH), 6-pyruvoyltetrahydropterin synthase (PTPS), pterin-4a-carbinolamine dehydratase (PCD), dihydropterin reductase (DHPR), and sepiapterin reductase (SR). However, the autosomal dominant from GTPCH and SR deficiencies do not cause hyperphenylalaninemia. (
  • Concurrently the World Health Organization (WHO) has begun a dialogue with scientists, clinicians, and manufacturers of malaria diagnostic test devices regarding the realistic possibilities for developing accurate, sensitive, and cost-effective rapid diagnostic tests for malaria. (
  • The majority of malaria cases are found in countries where cost-effectiveness is an important factor and ease of diagnostic test performance and training of personnel are also major considerations. (
  • An experimental diagnostic test for Alzheimer's disease analyzes the activity of an enzyme in skin. (
  • he xTAG CYP2C19 Kit v3 is an in vitro diagnostic test used to simultaneously detect and identify a panel of nucleotide variants found within the highly polymorphic CYP450 2C19 gene, located on chromosome 10q24, from genomic DNA extracted from EDTA or citrate anticoagulated whole blood samples. (
  • Talk to your provider about the meaning of your specific test results. (
  • Cytochrome P450 (CYP) enzyme-specific study agents (5-probe cocktail) will consist of oral doses of midazolam, warfarin/vitamin K, omeprazole, and caffeine administered on 4 separate occasions throughout the study. (
  • For this test, your health care provider or laboratory will give you a container and specific instructions on how to collect your samples at home. (
  • The Clinical Implications of Inconsistently Methylated Results from Glioblastoma MGMT Testing by Replicate Methylation-Specific PCR. (
  • 2.1.4 A "dedicated instrumentation" defines a situation where the assay must be run on a specific instrument as part of the testing protocol. (
  • Mammalian cell specific and sensitive serological test for cultures have many of the demerits dengue infections, also has major demerits. (
  • This device is used as an aid in determining treatment choice and individualizing treatment dose for therapeutics that are metabolized primarily by the specific enzyme about which the system provides genotypic information. (