An RNA-binding protein that recognizes the AAUAAA RNA SEQUENCE at the 3' end of MRNA. It contains four subunits of 30, 73, 100 and 160 kDa molecular size and combines with CLEAVAGE STIMULATION FACTOR to form a stable complex with mRNA that directs the 3' cleavage and polyadenylation reaction.
Factors that are involved in directing the cleavage and POLYADENYLATION of the of MESSENGER RNA near the site of the RNA 3' POLYADENYLATION SIGNALS.
The addition of a tail of polyadenylic acid (POLY A) to the 3' end of mRNA (RNA, MESSENGER). Polyadenylation involves recognizing the processing site signal, (AAUAAA), and cleaving of the mRNA to create a 3' OH terminal end to which poly A polymerase (POLYNUCLEOTIDE ADENYLYLTRANSFERASE) adds 60-200 adenylate residues. The 3' end processing of some messenger RNAs, such as histone mRNA, is carried out by a different process that does not include the addition of poly A as described here.
A RNA-binding protein that stimulates the cleavage of the 3' end of MRNA near the POLYADENYLATION site. It is a heterotrimer of 55-, 64- and 77-kDa subunits and combines with CLEAVAGE STIMULATION FACTOR to form a stable complex with mRNA that directs the 3' cleavage and polyadenylation reaction.
An enzyme that catalyzes the synthesis of polyadenylic acid from ATP. May be due to the action of RNA polymerase (EC 2.7.7.6) or polynucleotide adenylyltransferase (EC 2.7.7.19). EC 2.7.7.19.
The steps that generate the 3' ends of mature RNA molecules. For most mRNAs (RNA, MESSENGER), 3' end processing referred to as POLYADENYLATION includes the addition of POLY A.
RNA transcripts of the DNA that are in some unfinished stage of post-transcriptional processing (RNA PROCESSING, POST-TRANSCRIPTIONAL) required for function. RNA precursors may undergo several steps of RNA SPLICING during which the phosphodiester bonds at exon-intron boundaries are cleaved and the introns are excised. Consequently a new bond is formed between the ends of the exons. Resulting mature RNAs can then be used; for example, mature mRNA (RNA, MESSENGER) is used as a template for protein production.
Post-transcriptional biological modification of messenger, transfer, or ribosomal RNAs or their precursors. It includes cleavage, methylation, thiolation, isopentenylation, pseudouridine formation, conformational changes, and association with ribosomal protein.
A group of adenine ribonucleotides in which the phosphate residues of each adenine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Sequences found near the 3' end of MESSENGER RNA that direct the cleavage and addition of multiple ADENINE NUCLEOTIDES to the 3' end of mRNA.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A carboxy-lyase that plays a key role in photosynthetic carbon assimilation in the CALVIN-BENSON CYCLE by catalyzing the formation of 3-phosphoglycerate from ribulose 1,5-biphosphate and CARBON DIOXIDE. It can also utilize OXYGEN as a substrate to catalyze the synthesis of 2-phosphoglycolate and 3-phosphoglycerate in a process referred to as photorespiration.
A species of protozoa that is the causal agent of falciparum malaria (MALARIA, FALCIPARUM). It is most prevalent in the tropics and subtropics.
Complexes of RNA-binding proteins with ribonucleic acids (RNA).
Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.
Databases devoted to knowledge about specific genes and gene products.
The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.
Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).
A loose confederation of computer communication networks around the world. The networks that make up the Internet are connected through several backbone networks. The Internet grew out of the US Government ARPAnet project and was designed to facilitate information exchange.

Characterization of a specificity factor for an AAA+ ATPase: assembly of SspB dimers with ssrA-tagged proteins and the ClpX hexamer. (1/56)

SspB, a specificity factor for the ATP-dependent ClpXP protease, stimulates proteolysis of protein substrates bearing the ssrA degradation tag. The SspB protein is shown here to form a stable homodimer with two independent binding sites for ssrA-tagged proteins or peptides. SspB by itself binds to ClpX and stimulates the ATPase activity of this enzyme. In the presence of ATPgammaS, a ternary complex of SspB, GFP-ssrA, and the ClpX ATPase was sufficiently stable to isolate by gel-filtration or ion-exchange chromatography. This complex consists of one SspB dimer, two molecules of GFP-ssrA, and one ClpX hexamer. SspB dimers do not commit bound substrates to ClpXP degradation but increase the affinity and cooperativity of binding of ssrA-tagged substrates to ClpX, facilitating enhanced degradation at low substrate concentrations.  (+info)

Cellular antiviral responses against influenza A virus are countered at the posttranscriptional level by the viral NS1A protein via its binding to a cellular protein required for the 3' end processing of cellular pre-mRNAS. (2/56)

The influenza A virus NS1 protein (NS1A protein) binds and inhibits the function of the 30-kDa subunit of CPSF, a cellular factor that is required for the 3'-end processing of cellular pre-mRNAs. Here we generate a recombinant influenza A/Udorn/72 virus that encodes an NS1A protein containing a mutated binding site for the 30-kDa subunit of CPSF. This mutant virus is substantially attenuated, indicating that this binding site in the NS1A protein is required for efficient virus replication. Using this mutant virus, we show that NS1A binding to CPSF mediates the viral posttranscriptional countermeasure against the initial cellular antiviral response--the interferon-alpha/beta (IFN-alpha/beta)-independent activation of the transcription of cellular antiviral genes, which requires the interferon regulatory factor-3 (IRF-3) transcription factor that is activated by virus infection. Whereas the posttranscriptional processing of these cellular antiviral pre-mRNAs is inhibited in cells infected by wild-type influenza A virus, functional antiviral mRNAs are produced in cells infected by the mutant virus. These results establish that the binding of 30-kDa CPSF to the NS1A protein is largely responsible for the posttranscriptional inhibition of the processing of these cellular antiviral pre-mRNAs. Mutation of this binding site in the NS1A protein also affects a second cellular antiviral response: in cells infected by the mutant virus, IFN-beta mRNA is produced earlier and in larger amounts.  (+info)

tbCPSF30 depletion by RNA interference disrupts polycistronic RNA processing in Trypanosoma brucei. (3/56)

Gene expression in eukaryotes requires the post-transcriptional cleavage of mRNA precursors into mature mRNAs. In Trypanosoma brucei, mRNA processing is of particular importance, since most transcripts are derived from polycistronic transcription units. This organization dictates that regulated gene expression is promoter-independent and governed at the posttranscriptional level. We have identified tbCPSF30, a protein containing five CCCH zinc finger motifs, which is a homologue of the cleavage and polyadenylation specificity factor (CPSF) 30-kDa subunit, a component of the machinery required for 3'-end formation in yeast and mammals. Using gene silencing of tbCPSF30 by RNA interference, we demonstrate that this gene is essential in bloodstream and procyclic forms of T. brucei. Interestingly, tbCPSF30-specific RNA interference results in the accumulation of an aberrant tbCPSF30 mRNA species concomitant with depletion of tbCPSF30 protein. tbCPSF30 protein depletion is accompanied by the accumulation of unprocessed tubulin RNAs, implicating tbCPSF30 in polycistronic RNA processing. By genome data base mining, we also identify several other putative components of the T. brucei cleavage and polyadenylation machinery, indicating their conservation throughout eukaryotic evolution. This study is the first to identify and characterize a core component of the T. brucei CPSF and show its involvement in polycistronic RNA processing.  (+info)

Evidence that polyadenylation factor CPSF-73 is the mRNA 3' processing endonuclease. (4/56)

Generation of the polyadenylated 3' end of an mRNA requires an endonucleolytic cleavage followed by synthesis of the poly(A) tail. Despite the seeming simplicity of the reaction, more than a dozen polypeptides are required, and nearly all appear to be necessary for the cleavage reaction. Because of this complexity, the identity of the endonuclease has remained a mystery. Here we present evidence that a component of the cleavage-polyadenylation specificity factor CPSF-73 is the long-sought endonuclease. We first show, using site-specific labeling and UV-cross-linking, that a protein with properties of CPSF-73 is one of only two polypeptides in HeLa nuclear extract to contact the cleavage site in an AAUAAA-dependent manner. The recent identification of CPSF-73 as a possible member of the metallo-beta-lactamase family of Zn(2+)-dependent hydrolytic enzymes suggests that this contact may identify CPSF-73 as the nuclease. Supporting the significance of the putative hydrolytic lactamase domain in CPSF-73, we show that mutation of key residues predicted to be required for activity in the yeast CPSF-73 homolog result in lethality. Furthermore, in contrast to long held belief, but consistent with properties of metallo-beta-lactamases, we show that 3' cleavage is metal-dependent, likely reflecting a requirement for tightly protein-bound Zn(2+). Taken together, the available data provide strong evidence that CPSF-73 is the 3' processing endonuclease.  (+info)

Human immunodeficiency virus type 1 Tat increases the expression of cleavage and polyadenylation specificity factor 73-kilodalton subunit modulating cellular and viral expression. (5/56)

The human immunodeficiency virus type 1 (HIV-1) Tat protein, which is essential for HIV gene expression and viral replication, is known to mediate pleiotropic effects on various cell functions. For instance, Tat protein is able to regulate the rate of transcription of host cellular genes and to interact with the signaling machinery, leading to cellular dysfunction. To study the effect that HIV-1 Tat exerts on the host cell, we identified several genes that were up- or down-regulated in tat-expressing cell lines by using the differential display method. HIV-1 Tat specifically increases the expression of the cleavage and polyadenylation specificity factor (CPSF) 73-kDa subunit (CPSF3) without affecting the expression of the 160- and 100-kDa subunits of the CPSF complex. This complex comprises four subunits and has a key function in the 3'-end processing of pre-mRNAs by a coordinated interaction with other factors. CPSF3 overexpression experiments and knockdown of the endogenous CPSF3 by mRNA interference have shown that this subunit of the complex is an important regulatory protein for both viral and cellular gene expression. In addition to the known CPSF3 function in RNA polyadenylation, we also present evidence that this protein exerts transcriptional activities by repressing the mdm2 gene promoter. Thus, HIV-1-Tat up-regulation of CPSF3 could represent a novel mechanism by which this virus increases mRNA processing, causing an increase in both cell and viral gene expression.  (+info)

Structure of primate and rodent orthologs of the prostate cancer susceptibility gene ELAC2. (6/56)

The human ELAC2 gene was the first candidate prostate cancer susceptibility gene identified by linkage analysis and positional cloning. DNA sequence indicates a protein of 826 amino acids encoded by 24 exons. In the present study, we characterized the coding sequence of chimpanzee and gorilla ELAC2 orthologs by direct sequencing of genomic fragments, and of cynomolgus monkey and rat orthologs by screening cDNA libraries. The orthologs characterized in the chimpanzee, gorilla and cynomolgus monkey also encode proteins of 826 amino acids, sharing 98.9%, 98.5% and 93.7% sequence identity with the human protein. Our analyses of the mouse ELAC2 gene identified two alternative mRNA transcripts. One is translated into a protein of 824 a.a. (mouse ELAC2), whereas the other one encodes a protein of 831 amino acids (mouse ELAC2A) resulting from an alternatively spliced form of 25 exons. The rat ELAC2 gene ortholog also expressed two similar alternatively spliced transcripts. These two forms are ubiquitously expressed in mouse and rat tissues. The highest levels of expression of the ELAC2 form are observed in the testis while the lowest levels are seen in the prostate and in the muscle. However, it is of interest to note that the relative abundance of the rat and mouse ELAC2 transcripts, measured by real-time quantitative PCR, is higher than the respective ELAC2A forms in all surveyed tissues except for the prostate and the muscle. The ELAC2A transcript levels are 4.1 to 5.0-fold higher than the ELAC2 levels in the prostate of rat and mouse, respectively. A fine analysis of the conserved domains on the primary structure of ELAC2 orthologs revealed the presence of a putative beta-CASP domain shared by the PSO2 (SNM1) DNA interstrand cross-link repair proteins, and the 73-kDa subunit of mRNA 3' end cleavage and polyadenylation specificity factor (CPSF73) as well as Artemis proteins, thus suggesting a potential interaction of ELAC2 gene product with nucleic acids and more specifically with RNA targets. Taken together, these data offer useful tools to further study the regulation and cellular function of ELAC2 gene in experimental models and provide further insight concerning conserved amino acid motifs that could have biological significance.  (+info)

A CPSF-73 homologue is required for cell cycle progression but not cell growth and interacts with a protein having features of CPSF-100. (7/56)

Formation of the mature 3' ends of the vast majority of cellular mRNAs occurs through cleavage and polyadenylation and requires a cleavage and polyadenylation specificity factor (CPSF) containing, among other proteins, CPSF-73 and CPSF-100. These two proteins belong to a superfamily of zinc-dependent beta-lactamase fold proteins with catalytic specificity for a wide range of substrates including nucleic acids. CPSF-73 contains a zinc-binding histidine motif involved in catalysis in other members of the beta-lactamase superfamily, whereas CPSF-100 has substitutions within the histidine motif and thus is unlikely to be catalytically active. Here we describe two previously unknown human proteins, designated RC-68 and RC-74, which are related to CPSF-73 and CPSF-100 and which form a complex in HeLa and mouse cells. RC-68 contains the intact histidine motif, and hence it might be a functional counterpart of CPSF-73, whereas RC-74 lacks this motif, thus resembling CPSF-100. In HeLa cells RC-68 is present in both the cytoplasm and the nucleus whereas RC-74 is exclusively nuclear. RC-74 does not interact with CPSF-73, and neither RC-68 nor RC-74 is found in a complex with CPSF-160, indicating that these two proteins form a separate entity independent of the CPSF complex and are likely involved in a pre-mRNA processing event other than cleavage and polyadenylation of the vast majority of cellular pre-mRNAs. RNA interference-mediated depletion of RC-68 arrests HeLa cells early in G(1) phase, but surprisingly the arrested cells continue growing and reach the size typical of G(2) cells. RC-68 is highly conserved from plants to humans and may function in conjunction with RC-74 in the 3' end processing of a distinct subset of cellular pre-mRNAs encoding proteins required for G(1) progression and entry into S phase.  (+info)

The polyadenylation factor CPSF-73 is involved in histone-pre-mRNA processing. (8/56)

During 3' end processing, histone pre-mRNAs are cleaved 5 nucleotides after a conserved stem loop by an endonuclease dependent on the U7 small nuclear ribonucleoprotein (snRNP). The upstream cleavage product corresponds to the mature histone mRNA, while the downstream product is degraded by a 5'-3' exonuclease, also dependent on the U7 snRNP. To identify the two nuclease activities, we carried out UV-crosslinking studies using both the complete RNA substrate and the downstream cleavage product, each containing a single radioactive phosphate and a phosphorothioate modification at the cleavage site. We detected a protein migrating at 85 kDa that crosslinked to each substrate in a U7-dependent manner. Immunoprecipitation experiments identified this protein as CPSF-73, a known component of the cleavage/polyadenylation machinery. These studies suggest that CPSF-73 is both the endonuclease and 5'-3' exonuclease in histone-pre-mRNA processing and reveal an evolutionary link between 3' end formation of histone mRNAs and polyadenylated mRNAs.  (+info)

AtCPSF30 is the only Arabidopsis protein with a degree of sequence similarity to other eukaryotic CPSF30 proteins that extends beyond the typical spacing of Cys and His residues in the CCCH zinc-finger motif, and a number of lines of evidence support the conclusion that AtCPSF30 is an authentic polyadenylation factor subunit. As is the case with its yeast counterpart (Yth1p; Barabino et al., 1997), AtCPSF30 interacts with another Arabidopsis polyadenylation subunit homolog, AtFip1(V) (Forbes et al., 2006); AtFip1(V) also interacts with poly(A) polymerase and thereby provides a conceptual link between AtCPSF30 and poly(A) polymerase. The results presented in this study show that AtCPSF30 is present in the nucleus (Fig. 2B), as would be expected of a polyadenylation factor subunit. The coimmunoprecipitation of AtCPSF30 by antibodies raised against AtCPSF100 (Fig. 2C) indicates that AtCPSF30 resides, at least in part, in a complex with another Arabidopsis polyadenylation factor subunit. AtCPSF30 is ...
mRNA cleavage and polyadenylation specificity factor complex, 5-3 exonuclease activity, endoribonuclease activity, RNA binding, mRNA 3-end processing by stem-loop binding and cleavage, mRNA cleavage, mRNA polyadenylation
Cleavage factor Im (CFIm) is one of six factors necessary for correct cleavage and polyadenylation of pre-mRNAs. CFIm is composed of three different subunits of 25, 59, and 68 kDa, and it functions as a heterotetramer, with a dimer of the 25 kDa subunit binding to two of the 59 or 68 kDa subunits. The protein encoded by this gene represents the 59 kDa subunit, which can interact with the splicing factor U2 snRNP Auxiliary Factor (U2AF) 65 to link the splicing and polyadenylation complexes. [provided by RefSeq, Oct 2016 ...
Cleavage and polyadenylation specificity factor (CPSF) is relevant to the cleavage of the 3′ signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule. Specifically, it is in the process…. Read More Read More. ...
This gene encodes a subunit of the Integrator complex. This protein complex binds the C-terminal domain of RNA polymerase II and likely plays a role in small nuclear RNA processing. The encoded protein has similarities to the subunits of the cleavage and polyadenylation specificity factor complex. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010 ...
We have isolated the human homolog of S. cerevisiae Fip1p and have found that hFip1 is a genuine subunit of CPSF. Our results reported here indicate that hFip1 contributes to poly(A) site recognition and to CPSF‐dependent stimulation of polyadenylation. hFip1 binds to U‐rich sequence elements on the pre‐mRNA and is able to stimulate the activity of PAP.. CPSF has originally been described as a tetrameric complex containing the subunits CPSF160, CPSF100, CPSF73 and CPSF30, based on their stoichiometric cofractionation with CPSF activity. These polypeptides are related to four subunits of the yeast polyadenylation factor CPF (Yhh1p, Ydh1p, Ysh1p and Yth1p). However, CPF contains additional subunits (Preker et al, 1997; Dichtl et al, 2002a; Gavin et al, 2002; Walsh et al, 2002), one of which is Fip1p. Based on sequence conservation, we have identified the human homolog of yeast Fip1p and have shown that hFip1 is an additional, so far unrecognized subunit of CPSF. Most likely, hFip1 has not ...
Provides general gene info and summarizes expression data for all features of the gene SNAPC1 (aka ENSG00000023608, SNAPC1): snRNA-activating protein complex subunit 1 (SNAPc subunit 1)(snRNA-activating protein complex 43 kDa subunit)(SNAPc 43 kDa subunit)(Small nuclear RNA-activating complex polypeptide 1)(Proximal sequence element-binding transcription factor subunit gamma)(PSE-binding factor subunit gamma)(PTF subunit gamma) [Source:UniProtKB/Swiss-Prot;Acc:Q16533]
Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5 cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.
Complete information for CPSF4 gene (Protein Coding), Cleavage And Polyadenylation Specific Factor 4, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Complete information for JUND gene (Protein Coding), JunD Proto-Oncogene, AP-1 Transcription Factor Subunit, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
OphthoTech Corporation is developing pegpleranib, a 32-mer pegylated, anti-platelet derived growth factor subunit B (PDGF-B) aptamer, for the treatment of wet
CPSF3 Antibody 11609-1-AP has been identified with ELISA, IP, WB. 11609-1-AP detected 77 kDa band in HEK-293 cells with 1:500-1:2000 dilution...
CPSF30小鼠单克隆抗体[2202C1](ab51343)可与人样本反应并经WB, Dot实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
CPSF2兔多克隆抗体(ab100807)可与小鼠, 人样本反应并经IHC实验严格验证。中国75%以上现货,所有产品均提供质保服务,可通过电话、电邮或微信获得本地专属技术支持。
Kit Component:- KN200936G1, NUDT21 gRNA vector 1 in pCas-Guide vector- KN200936G2, NUDT21 gRNA vector 2 in pCas-Guide vector- KN200936D, donor vector…
Cleavage stimulatory factor or cleavage stimulation factor (CstF or CStF) is a heterotrimeric protein, made up of the proteins CSTF1 (55kDa), CSTF2 (64kDa) and CSTF3 (77kDa), totalling about 200 kDa. It is involved in the cleavage of the 3 signaling region from a newly synthesized pre-messenger RNA (mRNA) molecule. CstF is recruited by cleavage and polyadenylation specificity factor (CPSF) and assembles into a protein complex on the 3 end to promote the synthesis of a functional polyadenine tail, which results in a mature mRNA molecule ready to be exported from the cell nucleus to the cytosol for translation. The amount of CstF in a cell is dependent on the phase of the cell cycle, increasing significantly during the transition from G0 phase to S phase in mouse fibroblast and human splenic B cells. CSTF1, CSTF2, CSTF3 Martincic, K.; Campbell, R.; Edwalds-Gilbert, G.; Souan, L.; Lotze, M. T.; Milcarek, C. (1998). Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor ...
1. WahleE (1991) A novel poly(A)-binding protein acts as a specificity factor in the second phase of messenger RNA polyadenylation. Cell 66: 759-768.. 2. KuhnU, NemethA, MeyerS, WahleE (2003) The RNA binding domains of the nuclear poly(A)-binding protein. J Biol Chem 278: 16916-16925.. 3. KerwitzY, KuhnU, LilieH, KnothA, ScheuermannT, et al. (2003) Stimulation of poly(A) polymerase through a direct interaction with the nuclear poly(A) binding protein allosterically regulated by RNA. Embo J 22: 3705-3714.. 4. KuhnU, GundelM, KnothA, KerwitzY, RudelS, et al. (2009) Poly(A) tail length is controlled by the nuclear poly(A)-binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor. J Biol Chem 284: 22803-22814.. 5. KuhnU, WahleE (2004) Structure and function of poly(A) binding proteins. Biochim Biophys Acta 1678: 67-84.. 6. ApponiLH, LeungSW, WilliamsKR, ValentiniSR, CorbettAH, et al. (2010) Loss of nuclear poly(A)-binding protein 1 ...
GABPA (GA binding protein transcription factor subunit alpha), Authors: Dessen P. Published in: Atlas Genet Cytogenet Oncol Haematol.
Influenza virus NS1 protein is a nonstructural, multifunctional protein that counteracts host innate immune responses, modulating virus pathogenesis. NS1 protein variability in subjects infected with H3N2 influenza A viruses (IAVs) during the 2010/2011 season was analyzed, and amino acid changes in residues 86, 189, and 194 were found. The consequences of these mutations for the NS1-mediated inhibition of IFN responses and the pathogenesis of the virus were evaluated, showing that NS1 mutations D189N and V194I impaired the ability of the NS1 protein to inhibit general gene expression, most probably because these mutations decreased the binding of NS1 to the cleavage and polyadenylation specificity factor 30 (CPSF30). A recombinant A/Puerto Rico/8/34 (PR8) H1N1 virus encoding the H3N2 NS1-D189N protein was slightly attenuated, whereas the virus encoding the H3N2 NS1-V194I protein was further attenuated in mice. The higher attenuation of this virus could not be explained by differences in the ...
The PDB archive contains information about experimentally-determined structures of proteins, nucleic acids, and complex assemblies. As a member of the wwPDB, the RCSB PDB curates and annotates PDB data according to agreed upon standards. The RCSB PDB also provides a variety of tools and resources. Users can perform simple and advanced searches based on annotations relating to sequence, structure and function. These molecules are visualized, downloaded, and analyzed by users who range from students to specialized scientists.
Reaktivität: Fledermaus, Huhn, Rind (Kuh) and more. 67 verschiedene CPSF4 Antikörper vergleichen. Alle direkt auf antikörper-online bestellbar!
Abnova Human CPSF3 Partial ORF (NP_057291, 585 a.a. - 684 a.a.) Recombinant Protein with GST-tag at N-terminal 25µg Life Sciences:Protein Biology:Proteins:Proteins A-Z:Proteins
Besides the splice site itself, only two sequences on the pre-mRNA play an important role in SL2-specific trans-splicing: the two presumptive signals for 3 end formation of the gene just upstream (Figure 2; Huang et al., 2001; Kuersten et al., 1997; Liu et al., 2001; Liu et al., 2003). The AAUAAA just 5 of the cleavage site, which is absolutely required for 3 end formation, binds the cleavage and polyadenylation specificity factor (CPSF), and a U-rich sequence just 3 of the cleavage site binds the cleavage stimulatory factor (CstF). CPSF and CstF bind cooperatively to these two sites and together position the site of cleavage. When the AAUAAA is mutated, 3 end cleavage fails to occur, and trans-splicing just downstream becomes less efficient and less specific for SL2. Nevertheless, AAUAAA is not required for SL2 trans-splicing since SL2 trans-splicing downstream still occurs in its absence. CPSF bound to AAUAAA may act by facilitating binding of CstF to the U-rich sequence or by catalyzing ...
Gentaur molecular products has all kinds of products like :search , GenWay \ Cleavage stimulation factor 50 kDa subunit - CSTF 50 kDa subunit; CF-1 50 kDa subunit; CstF-50 \ 10-288-22329F for more molecular products just contact us
Dr. Verma research involves understanding the pathogenesis of tumor viruses, specifically Epstein Barr Virus (EBV) and Kaposis sarcoma associated herpesvirus (KSHV). Both viruses undergo lytic replication in epithelial cells during primary infection and during reactivation from latent infection in B-lymphocytes. Epstein Barr virus SM is an RNA binding protein essential for viral replication that enhances EBV gene expression by enhancing RNA stability and RNA export. Dr. Verma has shown that SM interacts with cellular splicing factors and influences splicing of both EBV and cellular pre-mRNAs. Like EBV SM, KSHV ORF57 is also a post-transcriptional regulatory protein, essential for KSHV lytic replication and has high degree of gene specificity. His current research is focused on regulation of gene expression and antiviral drug screening in the following areas ...
A maquinaria de poliadenilación no núcleo de eucariotas actúa sobre os produtos orixinados pola ARN polimerase II, como son os ARNm precursores. Aquí, un complexo multiproteico (cuxos compoñentes poden verse na táboa da dereita) clivan a parte final do extremo 3 do ARN que se acaba de formar, e poliadenila o extremo que se orixina por esta clivaxe. A clivaxe está catalizada polo encima CPSF[7] e ten lugar 10-30 nucleótidos corrente abaixo do seu sitio de unión.[12] Este sitio é xeralmente a secuencia AAUAAA do ARN, pero hai variantes del aos que tamén se une máis debilmente o CPSF.[13] Outras dúas proteínas que engaden especificidade á unión a un ARN son CstF e CFI. O CstF únese a unha rexión rica en GU situada máis corrente abaixo do sitio do CPSF.[14] O CFI recoñece un terceiro sitio no ARN (un conxunto de secuencias UGUAA en mamíferos[15][16][17]) e pode recrutar o CPSF mesmo se se perde a secuencia AAUAAA.[18][19] O sinal de poliadenilación (a secuencia motivo ...
CPSF3 Polyclonal Antibody from Invitrogen for Western Blot applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (1 mg/ml) in PBS with 1% BSA, 20% glycerol and 0.01% thimerosal; pH 7.
Recombinant protein from the full-length sequence of homo sapiens cleavage and polyadenylation factor I subunit 1 (CLP1), transcript variant 1 (NM_006831), with a His tag., from EUPROTEIN
BACKGROUND: Expression of the cellular karyopherin TNPO3/transportin-SR2/Tnp3 is necessary for HIV-1 infection. Depletion of TNPO3 expression in mammalian
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the protein family comprised of both platelet-derived growth factors (PDGF) and vascular endothelial growth factors (VEGF). The encoded preproprotein is proteolytically processed to generate platelet-derived growth factor subunit A, which can homodimerize, or alternatively, heterodimerize with the related platelet-derived growth factor subunit B. These proteins bind and activate PDGF receptor tyrosine kinases, which play a role in a wide range of developmental processes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015 ...
Global antibody supplier and research reagent supplier to the life science community. Find antibodies and reagents all backed by our Guarantee+.
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
EE, PE and poly(A)‐site signals were suggested to be sufficient to direct cleavage and polyadenylation of yeast pre‐mRNAs (Guo and Sherman, 1996b). CF IA, CF IB and CF II trans‐acting factors were initially defined as sufficient for cleavage in vitro (Kessler et al., 1996). Subsequent work showed, however, that CF IB is dispensable for cleavage activity and instead controls cleavage‐site selection (Minvielle‐Sebastia et al., 1998). These observations also resulted in the surprising finding that CYC1‐512 RNA is cleaved by CF IA and CF II in vitro when CF IB is absent, suggesting that both EE and PE are dispensable for cleavage.. We extended this observation by analysing cleavage of a short CYC1 substrate by CPF and CF IA in vitro. Consistent with previous results CF IB was not required for cleavage activity but restricted cleavage to the poly(A) site. While deletion of EE sequences in sCYC1 did not influence poly(A)‐site cleavage, removal of the PE had a strong effect. In contrast, ...
Cstf3 - Cstf3 (GFP-tagged) - Mouse cleavage stimulation factor 3 pre-RNA subunit 3 (Cstf3) transcript variant 2, (10ug) available for purchase from OriGene - Your Gene Company.
Cstf2 - Lenti ORF clone of Cstf2 (mGFP-tagged) - Mouse cleavage stimulation factor, 3 pre-RNA subunit 2 (cDNA clone MGC:36412 IMAGE:5322335) available for purchase from OriGene - Your Gene Company.
Exogenous human granulocyte-colony stimulation factor (huG-CSF) restores autoantibody (autoAb) production in B6.Sle2c2 mice after induction of chronic graft vs
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. The Cul4A gene is amplified in human breast and liver cancers, and loss-of-function of Cul4 re …
BACKGROUND: Expression of the cellular karyopherin TNPO3/transportin-SR2/Tnp3 is necessary for HIV-1 infection. Depletion of TNPO3 expression in mammalian
The process of 3′ end formation/polyadenylation occurs co-transcriptionally on cellular and HIV mRNAs generated by RNA Pol II and influences the termination of transcription at a site several hundred bases downstream of the mature 3′ end of the mRNA [50]. The 3′ end of most human mRNAs is generated first by an endonucleolytic cleavage event (catalyzed by CPSF73, aka CPSF3) followed by the addition of 100-250 adenylate residues by poly(A) polymerase (PAP). A typical polyadenylation signal contains two types of elements. The core elements consist of an AAUAAA or similar hexanucleotide and a short (about 5 base long) U- or GU-rich tract located within approximately 25-30 bases upstream or downstream, respectively, of the site. The core elements serve as the assembly site of the complex of polyadenylation factors. Many polyadenylation signals also contain auxiliary elements that are located upstream or downstream of the core elements. These auxiliary elements bind to a variety of cellular ...
Abstract. Previously we found that haplotype 2 of the fibrinogen gamma gene (FGG-H2) is associated with an increased risk of deep venous thrombosis and with red
Human CSTF2 full-length ORF ( AAH17712, 1 a.a. - 577 a.a.) recombinant protein with GST-tag at N-terminal. (H00001478-P01) - Products - Abnova
chr5:142990567-143005539, + strand. Annotation of mouse strain PWK/PhJ genome assembly provided by GENCODE consortium. Distributed via Ensembl Release 92. Gene type: protein coding gene; Gene Name: Cpsf4 ...
Available drugs to treat toxoplasmosis have limitations in efficacy and can cause serious adverse effects. Moreover, toxoplasmosis is now recognized as a leading cause of foodborne illness in the United States (Scallan et al, 2011). Since a Toxoplasma vaccine for use in humans is not currently available, new classes of drugs, preferably directed against novel targets, are needed. Here we report that the benzoxaborole AN3661 inhibits Toxoplasma growth in vitro and, when orally administered to mice, is not only effective against otherwise lethal infections but also enables protective immunity against subsequent Toxoplasma infections. Genetic evidence reported herein supports the conclusion that AN3661 acts via the inhibition of a novel target of T. gondii, TgCPSF3, which is homologous to the endonuclease subunit (CPSF‐73) within the human CPSF complex that cleaves 3′‐mRNAs (Ryan et al, 2004; Mandel et al, 2006).. In another study, it is shown that AN3661 is also active against the human ...
Available drugs to treat toxoplasmosis have limitations in efficacy and can cause serious adverse effects. Moreover, toxoplasmosis is now recognized as a leading cause of foodborne illness in the United States (Scallan et al, 2011). Since a Toxoplasma vaccine for use in humans is not currently available, new classes of drugs, preferably directed against novel targets, are needed. Here we report that the benzoxaborole AN3661 inhibits Toxoplasma growth in vitro and, when orally administered to mice, is not only effective against otherwise lethal infections but also enables protective immunity against subsequent Toxoplasma infections. Genetic evidence reported herein supports the conclusion that AN3661 acts via the inhibition of a novel target of T. gondii, TgCPSF3, which is homologous to the endonuclease subunit (CPSF‐73) within the human CPSF complex that cleaves 3′‐mRNAs (Ryan et al, 2004; Mandel et al, 2006).. In another study, it is shown that AN3661 is also active against the human ...
Enterotoxigenic E. coli (ETEC) contribute significantly to the substantial burden of infectious diarrhea among children living in low and middle income countries. In the absence of a vaccine for ETEC, children succumb to acute dehydration as well as non-diarrheal sequelae related to these infections including malnutrition. The considerable diversity of ETEC genomes has complicated canonical vaccine development approaches defined by a subset of ETEC pathovar-specific antigens known as colonization factors (CFs). To identify additional conserved immunogens unique to this pathovar we employed an open-aperture approach to capture all potential conserved ETEC surface antigens in which we mined genomic sequences of 89 ETEC isolates, bioinformatically selected potential surface-exposed pathovar-specific antigens conserved in more than 40% of the genomes (n=118), and assembled the representative proteins onto microarrays, complemented with known or putative colonization factor subunit molecules ...
Constitutive STAT3 activation by tyrosine phosphorylation of mutated or amplified tyrosine kinases (pYSTAT3) is critical for cancer initiation, progression, invasion, and motility of carcinoma cells. We showed that AF1q is associated with STAT3 signaling in breast cancer cells. In xenograft models, enhanced AF1q expression activated STAT3 and promoted tumor growth and metastasis in immunodeficient NSG mice. The cytokine secretory phenotype of MDA-MB-231LN breast cancer cells with altered AF1q expression revealed changes in expression of platelet-derived growth factor subunit B (PDGF-B). AF1q-induced PDGF-B stimulated motility, migration, and invasion of MDA-MB-231LN cells, and AF1q up-regulated platelet-derived growth factor receptor (PDGFR) signaling. Further, AF1q-induced PDGFR signaling enhanced STAT3 activity through Src kinase activation, which could be blocked by the Src kinase inhibitor PP1. Moreover, AF1q up-regulated tyrosine kinase signaling through PDGFR signaling, which was blockable by
IPF is a deadly disease with a high prevalence (14.0-42.7 per 100,000 persons) and low survival rate (20%-40% 5-year survival rate) (1). The proliferation and activation of ECM-producing myofibroblasts is a central but not yet fully understood process that contributes to the progression of IPF. Thus, the identification of targets that promote abnormal myofibroblast proliferation and differentiation or enhance profibrotic gene expression could prove highly beneficial in designing therapies for pulmonary fibrosis. In our study, we explored the expression of a key APA complex in pulmonary fibrosis and showed, for the first time to our knowledge, that there is a significant decrease of CFIm components (CFIm25, CPSF59, and CPSF68) in myofibroblasts from the lungs of patients with IPF and mice with pulmonary fibrosis, suggesting a possible global 3′-UTR shortening during fibroblast-to-myofibroblast differentiation. In addition, downregulation of CFIm25 was sufficient to shorten the 3′-UTR of ...
CSTF Resuscitation Adults Levels 1, 2 & 3 - This course is a mandatory requirement for those who work in health and social care services, including but not l...
Rabbit polyclonal CPSF7 antibody. Validated in WB, ELISA, IHC and tested in Human. Cited in 1 publication(s). Immunogen corresponding to synthetic peptide.
Much like using dimmer switches to brighten or darken rooms, biochemists have identified a protein called CFIm25 that can be used to slow down or speed up the growth of brain tumors in mice.
Use Bio-Rads PrimePCR assays, controls, templates for your target gene. Every primer pair is optimized, experimentally validated, and performance guaranteed.
At the effector domain, it interacts and inhibits cleavage and polyadenylation specificity factor (CPSF30). CPSF30 is part of ... It prevents polyadenylation of cellular mRNAs to circumvent antiviral responses of the host, e.g., maturation and translation ... This kinase phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (elF-2 alpha), leading to a decrease ... double-stranded RNA inhibits the activation of the protein kinase that phosphorylates the elF-2 translation initiation factor. ...
Cleavage and polyadenylation specificity factor subunit 4 is a protein that in humans is encoded by the CPSF4 gene. Inhibition ... 2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO ... "Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit". Mol. Cell. Biol ... Cleavage and polyadenylation specificity factor subunit 4) at the PDBe-KB. v t e. ...
Cleavage and polyadenylation specificity factor subunit 2 is a protein that in humans is encoded by the CPSF2 gene. GRCh38: ... Jenny A, Hauri HP, Keller W (Dec 1994). "Characterization of cleavage and polyadenylation specificity factor and cloning of its ... Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'- ... 2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO ...
Murthy KG, Manley JL (November 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre- ... Cleavage stimulation factor 50 kDa subunit is a protein that in humans is encoded by the CSTF1 gene. This gene encodes one of ... "Entrez Gene: CSTF1 cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa". Kleiman FE, Manley JL (September 1999). " ... three subunits which combine to form cleavage stimulation factor (CSTF). CSTF is involved in the polyadenylation and 3' end ...
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'- ... cleavage stimulation factors that combine to form the cleavage stimulation factor complex (CSTF). This complex is involved in ... Cleavage stimulation factor 77 kDa subunit is a protein that in humans is encoded by the CSTF3 gene. The protein encoded by ... "Entrez Gene: CSTF3 cleavage stimulation factor, 3' pre-RNA, subunit 3, 77kDa". Takagaki, Y; Manley J L (March 2000). "Complex ...
... namely cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), cleavage factors Im and IIm ... Cleavage and polyadenylation specificity factor subunit 1 is a protein that in humans is encoded by the CPSF1 gene. In most ... 2002). "Cleavage and polyadenylation specificity factor (CPSF)-derived peptides can induce HLA-A2-restricted and tumor-specific ... Murthy KG, Manley JL (Dec 1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA ...
Cleavage and polyadenylation specificity factor subunit 7 is a protein that in humans is encoded by the CPSF7 gene. CPSF7, also ... CPSF7 is one of three Cleavage and polyadenylation specificity factors (CPSF), the other two being CFIm25 (or CPSF5/NUDT21) and ... "Entrez Gene: FLJ12529 pre-mRNA cleavage factor I, 59 kDa subunit". Hardy JG, Norbury CJ (August 2016). "Cleavage factor Im ( ... Rüegsegger U, Beyer K, Keller W (March 1996). "Purification and characterization of human cleavage factor Im involved in the 3 ...
Cleavage and polyadenylation specificity factor subunit 6 is a protein that in humans is encoded by the CPSF6 gene. The protein ... "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". The EMBO ... "Entrez Gene: CPSF6 cleavage and polyadenylation specific factor 6, 68kDa". Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, ... Awasthi S, Alwine JC (Nov 2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA. 9 (11): 1400-9. doi: ...
Likewise, it interacts with cytoplasmic polyadenylation factors, including Cleavage and polyadenylation specificity factor and ... which is a process known as polyadenylation. For RNA specificity, GLD-2 associates with an RNA-binding protein, typically a GLD ... The polyadenylation activity of GLD-2, as we previously mentioned, is stimulated by physical interaction with an RNA binding ... PAP heterodimer in which GLD-2 contains the active site and GLD-3 provides RNA-binding specificity. MS2 coat protein was joined ...
Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is an enzyme that in humans is encoded by the NUDT21 gene. It ... 2000). "Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J ... Awasthi S, Alwine JC (2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA. 9 (11): 1400-9. doi:10.1261 ... The protein encoded by this gene is one subunit of the cleavage factor Im complex required for 3' RNA cleavage and ...
... cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome ...
... binds to FIP1L1 (Factor interacting with PAPOLA and CPSF1), a subunit of the cleavage and polyadenylation specificity ... 2005). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein ... Pendurthi UR, Alok D, Rao LV (1997). "Binding of factor VIIa to tissue factor induces alterations in gene expression in human ... end-processing factor. FIP1L1 gene fusions between it and either the platelet-derived growth factor receptor, alpha (PGDFRA) or ...
Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'- ... 1998). "Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition ... The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and ... Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a ...
... is a subunit of the cleavage and polyadenylation specificity factor subunit 1 (CPSF1) complex that polyadenylates the 3 ... Factor interacting with PAPOLA and CPSF1 (i.e, FIP1L1; also termed Pre-mRNA 3'-end-processing factor FIP1) is a protein that in ... end-processing factor. FIP1L1 gene fusions between it and either the platelet-derived growth factor receptor, alpha (PGDFRA) or ... "Pre-mRNA 3'-end-processing factor FIP1 (Q6UN15) < InterPro < EMBL-EBI". www.ebi.ac.uk. Vega F, Medeiros LJ, Bueso-Ramos CE, ...
... the proteins cleavage and polyadenylation specificity factor (CPSF) and cleavage stimulation factor (CstF) transfer from the ... These two factors then recruit other proteins to the site to cleave the transcript, freeing the mRNA from the transcription ... After this cleavage, a so-called exonuclease binds to the residual RNA strand and removes the freshly transcribed nucleotides ... Termination codon Termination factor Terminator gene Transcription (genetics) Richardson, J. P. (1996). "Rho-dependent ...
Cleavage and polyadenylation specificity factor subunit 7 CPT1A: carnitine palmitoyltransferase 1A (liver) CREBZF encoding ... Yip1 interacting factor homolog A ZFP91-CNTF ZNF408: zinc finger protein 408 The following diseases and disorders are some of ... remodeling and spacing factor 1 SAA1: serum amyloid A1 SAA2: serum amyloid A2 SAC3D1: SAC3 domain-containing protein 1 SART1: ... tumor necrosis factor recepteor REXO2: RNA exonuclease 2 RNH1: ribonuclease inhibitor 1 RNU2-2: encoding protein RNA, U2 small ...
The transfer of these sequence specific binding proteins cleavage and polyadenylation specificity factor (CPSF), Cleavage ... Cleavage and polyadenylation specificity factor 73 cuts mRNA between stem-loop and HDE Histone variants, such as H2A.Z or H3.3 ... Factor I (CF I) and cleavage stimulation factor (CStF) occurs from RNA Polymerase II. The three factors bind to the sequence ... processing is done independent of most cleavage and polyadenylation factors. Core histone mRNAs have a special stem-loop ...
... to a large protein complex that also contains smaller assemblies known as the cleavage and polyadenylation specificity factor ( ... CPSF) and cleavage stimulatory factor (CtSF) and its binding is a necessary prerequisite to the cleavage of the 3' end of the ... After cleavage of the 3' signaling region that directs the assembly of the complex, polyadenylate polymerase (PAP) adds the ... PAP is phosphorylated by mitosis-promoting factor, a key regulator of the cell cycle. High phosphorylation levels decrease PAP ...
... complexes are recruited to the cleavage site by cleavage and polyadenylation specificity factor and cleavage stimulatory factor ... Cleavage factors are two closely associated protein complexes involved in the cleavage of the 3' untranslated region of a newly ... "Cleavage factor Im (CFIm) as a regulator of alternative polyadenylation". Biochemical Society Transactions. 44 (4): 1051-1057. ... In mammals the two cleavage factors are known as CFIm and CFIIm. The proteins that constitute these ...
CstF is recruited by cleavage and polyadenylation specificity factor (CPSF) and assembles into a protein complex on the 3' end ... Cleavage stimulatory factor or cleavage stimulation factor (CstF or CStF) is a heterotrimeric protein, made up of the proteins ... "Increase in the 64-kDa subunit of the polyadenylation/cleavage stimulatory factor during the G0 to S phase transition". ... Cleavage+stimulation+factor at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ...
... cleavage and polyadenylation specificity factor) and CSTF (cleavage stimulation factor), recognize the poly-A signal in the ... Poly-A-bound CPSF and CSTF recruit other proteins to carry out RNA cleavage and then polyadenylation. Poly-A polymerase adds ... General transcription factors are a group of proteins involved in transcription initiation and regulation. These factors ... There are different classes of elongation factors. Some factors can increase the overall rate of transcribing, some can help ...
... (CPSF) is involved in the cleavage of the 3' signaling region from a newly ... Cleavage+And+Polyadenylation+Specificity+Factor at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... Murthy, KG; Manley, JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3 ... "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes & ...
... cleavage and polyadenylation specificity factor MeSH D12.776.664.962.444.249 - cleavage stimulation factor MeSH D12.776.664.962 ... vascular endothelial growth factor a MeSH D12.776.467.100.800.300 - vascular endothelial growth factor b MeSH D12.776.467.100. ... fibroblast growth factor 4 MeSH D12.776.624.664.700.112 - fibroblast growth factor 6 MeSH D12.776.624.664.700.114 - fms-like ... peptide elongation factor g MeSH D12.776.835.700.350.700 - peptide elongation factor tu MeSH D12.776.835.700.350.800 - peptide ...
Wahle, E.; Keller, W. (1992). "The Biochemistry of 3-End Cleavage and Polyadenylation of Messenger RNA Precursors". Annual ... Lamfrom, Hildegard (June 1961). "Factors determining the specificity of hemoglobin synthesized in a cell-free system". Journal ... They function by binding to specific sites within the mRNA and inducing cleavage of the mRNA via a specific silencing- ... Studies of transcription also led to the identification of many protein factors that influence transcription, including ...
Genes, factors and transcriptional specificity". Eur. J. Biochem. 212 (1): 1-11. doi:10.1111/j.1432-1033.1993.tb17626.x. PMID ... Transcription termination in eukaryotes is less well understood than in bacteria, but involves cleavage of the new transcript ... followed by template-independent addition of adenines at its new 3' end, in a process called polyadenylation.[21] ... A sigma (σ) factor binds to the core, forming the holoenzyme. After transcription starts, the factor can unbind and let the ...
... cleavage stimulation factor MeSH D12.776.157.725.124.500 - cleavage and polyadenylation specificity factor MeSH D12.776.157.725 ... MeSH D12.776.157.057.030.124 - interferon regulatory factor-1 MeSH D12.776.157.057.030.249 - interferon regulatory factor-2 ... peptide elongation factor g MeSH D12.776.157.325.150.700 - peptide elongation factor tu MeSH D12.776.157.325.150.800 - peptide ... adp-ribosylation factors MeSH D12.776.157.325.515.100.100 - adp-ribosylation factor 1 MeSH D12.776.157.325.515.400 - rab gtp- ...
... end cleavage, and polyadenylation. Ser5 phosphorylation (Ser5PO4) near the 5′ ends of genes depends principally on the kinase ... General transcription factors (GTFs) or basal transcription factors are protein transcription factors that have been shown to ... Nevertheless, TBP is always involved and is forced to bind without sequence specificity. TAFs from TFIID can also be involved ... Chromatin structure-oriented factors are more complex than for initiation control. Often the chromatin-altering factor becomes ...
C3a is a neutrophil chemotactic factor which operates through a G protein coupled chemotactic factor receptor, the C3a receptor ... The latter two receptors were subsequently found to have very different specificities for the formyl oligopeptides and very ... Furthermore, bacteria-induced complement activation also causes the formation of complement component 3a (C3a) by cleavage from ... alternate Polyadenylation sites, and possibly products of other genes with homology to FPR1. Subsequent studies cloned two ...
3′ cleavage/polyadenylation and 5' capping increase overall RNA stability, and the presence of 5' cap allows ribosome binding ... trans-acting factors), which may modify or regulate the expression of distant genes. Trans-acting factors interact with cis- ... degradation or cleavage of mRNA. Certain mRNA binding mechanisms have high specificity, which can act as a form of the ... Trans-acting factors can be categorized by their interactions with the regulated genes, cis-acting elements of the genes, or ...
Hoshino S, Imai M, Kobayashi T, Uchida N, Katada T (June 1999). "The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) ... Huang Y, Carmichael GG (April 1996). "Role of polyadenylation in nucleocytoplasmic transport of mRNA". Molecular and Cellular ... and RNA-binding specificity". Experimental Cell Research. 211 (2): 400-7. doi:10.1006/excr.1994.1104. PMID 7908267. Campbell LH ... and viral protease-mediated cleavages". The Journal of Biological Chemistry. 276 (26): 23922-8. doi:10.1074/jbc.M100384200. ...
The specificity of these transcription factors' interactions with DNA come from the proteins making multiple contacts to the ... There are two types of cleavage: east-west cleavage and north-south cleavage. The north-south cleavage nicks both strands of ... It may act as a recognition factor to regulate the attachment and dispersal of specific cell types in the biofilm;[102] it may ... Each transcription factor binds to one particular set of DNA sequences and activates or inhibits the transcription of genes ...
RNase specificityEdit. The active site looks like a rift valley where all the active site residues create the wall and bottom ... Sporn MB, Roberts AB (6 December 2012). Peptide Growth Factors and Their Receptors II. Springer Science & Business Media. p. ... RNase H is a non-specific endonuclease and catalyzes the cleavage of RNA via a hydrolytic mechanism, aided by an enzyme-bound ... end polyadenylation, and folding within an RNA protein complex (ribonucleoprotein particle or RNP). ...
... correct cleavage of the von Willebrand factor precursor at a paired basic amino acid site". Proceedings of the National Academy ... "cDNA and gene structure for a human subtilisin-like protease with cleavage specificity for paired basic amino acid residues". ... The use of alternate polyadenylation sites has been found for this gene.[7] ... nerve growth factor processing. • nerve growth factor production. • cellular proliferation. • transforming growth factor beta ...
For instance, cleavage of laminin-5 by cathepsin S leads to generation of proangiogenic peptides. The expression of cathepsin S ... Hall A, Håkansson K, Mason RW, Grubb A, Abrahamson M (March 1995). "Structural basis for the biological specificity of cystatin ... Cathepsin S has been shown to be a significant prognostic factor for patients with type IV astrocytomas (glioblastoma ... Transcript variants utilizing alternative polyadenylation signals exist for this gene. Cathepsin S is a member of the peptidase ...
... has a specificity similar to that of chymotrypsin C, but it is most closely related to other immune serine ... Other functions of cathepsin G have been reported, including cleavage of receptors, conversion of angiotensin I to angiotensin ... Transcript variants utilizing alternative polyadenylation signals exist for this gene. Cathepsin G was also found to exert ... Ib-IX complex by proteolysis of the von Willebrand factor binding site on GPIb alpha and by a cytoskeletal-mediated ...
Yang Q, Coseno M, Gilmartin GM, Doublié S (March 2011). "Crystal structure of a human cleavage factor CFI(m)25/CFI(m)68/RNA ... Two other proteins add specificity to the binding to an RNA: CstF and CFI. CstF binds to a GU-rich region further downstream of ... Cleavage also involves the protein CFII, though it is unknown how. The cleavage site associated with a polyadenylation signal ... The polyadenylation signal - the sequence motif recognised by the RNA cleavage complex - varies between groups of eukaryotes. ...
"Positional specificity of different transcription factor classes within enhancers". Proceedings of the National Academy of ... CAAT box CCAAT box Operator (biology) Pribnow box TATA box SECIS element, mRNA Polyadenylation signals, mRNA A-box Z-box C-box ... In RNA, regulation may occur at the level of protein biosynthesis (translation), RNA cleavage, RNA splicing, or transcriptional ... An inactive enhancer may be bound by an inactive transcription factor. Phosphorylation of the transcription factor may activate ...
Complex: Cleavage and polyadenylation specificity factor complex Macromolecular complex annotations are imported from the ...
Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3 signaling region from a newly ... Cleavage+And+Polyadenylation+Specificity+Factor at the US National Library of Medicine Medical Subject Headings (MeSH) v t e. ... Murthy, KG; Manley, JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3 ... "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3-end formation". Genes & ...
Cleavage and polyadenylation specif.... Cleavage and polyadenylation specificity factor subunit 1 (Cleavage and polyadenylation ... Cleavage and polyadenylation-specificity factor subunit 1Imported. ,p>Information which has been imported from another database ... tr,E9PIM1,E9PIM1_HUMAN Cleavage and polyadenylation-specificity factor subunit 1 OS=Homo sapiens OX=9606 GN=CPSF1 PE=1 SV=3 ... Cleavage and polyadenylation specificity factor subunit 1 (Fragment). Bengalese finch. 163. UniRef50_A0A218U8Z9. ...
DIMERIC ARCHAEAL CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR WITH N-TERMINAL KH DOMAINS (KH-CPSF) FROM METHANOSARCINA MAZEI ... CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR 100 KD SUBUNIT. A, B. 640. Methanosarcina mazei (strain ATCC BAA-159 / DSM 3647 ... DIMERIC ARCHAEAL CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR WITH N-TERMINAL KH DOMAINS (KH-CPSF) FROM METHANOSARCINA MAZEI ... Crystal Structure of a Dimeric Archaeal Cleavage and Polyadenylation Specificity Factor.. Mir-Montazeri, B., Ammelburg, M., ...
... end processing by stem-loop binding and cleavage, mRNA cleavage, mRNA polyadenylation ... mRNA cleavage and polyadenylation specificity factor complex, 5-3 exonuclease activity, endoribonuclease activity, RNA ... mRNA cleavage and polyadenylation specificity factor complex Source: GeneDBInferred from sequence or structural similarityi*. " ... Cleavage and polyadenylation specificity factor subunit 3, putativeImported. ,p>Information which has been imported from ...
Cellular Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Mediates Nuclear Import of Human Bocavirus 1 NP1 Protein and ... Cellular cleavage and polyadenylation specificity factor 6 (CPSF6) mediates nuclear import of human bocavirus 1 NP1 protein and ... Cellular Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Mediates Nuclear Import of Human Bocavirus 1 NP1 Protein and ... Cellular Cleavage and Polyadenylation Specificity Factor 6 (CPSF6) Mediates Nuclear Import of Human Bocavirus 1 NP1 Protein and ...
Binds to cleavage and polyadenylation RNA substrates. Cleavage factor Im (CFIm) is one of six factors necessary for correct ... Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation.. ... Tandem affinity purification and mass spectrometry analysis identify 59kDa cleavage and polyadenylation specific factor 7 ( ... Tandem affinity purification and mass spectrometry analysis identify 59kDa cleavage and polyadenylation specific factor 7 ( ...
Binds to cleavage and polyadenylation RNA substrates. Cleavage factor Im (CFIm) is one of six factors necessary for correct ... HCA RNA Cell Line for Cleavage and polyadenylation specificity factor subunit 7. ... which can interact with the splicing factor U2 snRNP Auxiliary Factor (U2AF) 65 to link the splicing and polyadenylation ... Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3-processing. ...
A potent antimalarial benzoxaborole targets a Plasmodium falciparum cleavage and polyadenylation specificity factor homologue * ... cleavage and polyadenylation specificity factor homologue . Opens in a new window. ...
Guinea pig Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) ELISA Kit MyBioSource.com ... Bovine Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) ELISA Kit MyBioSource.com ... Canine Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) ELISA Kit MyBioSource.com ... Chicken Cleavage and polyadenylation specificity factor subunit 6 (CPSF6) ELISA Kit MyBioSource.com ...
Cleavage and polyadenylation specificity factor subunit 4. Drosophila melanogaster (Fruit fly). Loading... ...
alternative polyadenylation. CPSF. cleavage and polyadenylation specificity factor. CstF. cleavage stimulation factor. GO. gene ... containing the cleavage stimulation factor (CstF) and the cleavage and polyadenylation specificity factor (CPSF), can identify ... The cleavage and polyadenylation of the pre-mRNA 3′ end are determined by the interaction of cis-acting elements with various ... Analysis of alternative cleavage and polyadenylation by 3′ region extraction and deep sequencing. Nat. Methods 10: 133-139. ...
GO:0098789 pre-mRNA cleavage required for polyadenylation Molecular Function. None predicted. ...
... cleavage and polyadenylation specificity factor complex; APC: anaphase-promoting complex; CTLH: C-terminal to LisH motif ... Tubulin was used as a loading and IP specificity control.. (C-D) iSLK cells infected with WT KSHV or KSHV.24.Stop were ... GAPDH was used as a loading and IP specificity control. In this and all subsequent IPs, input represents 5% of lysate used for ... Where indicated, the TATT motif in the K8.1 promoter probe was mutated to CCCC. The specificity of the interaction was ...
A protein complex that was affinity-purified by SYM10 but not SYM11 was the cleavage and polyadenylation specificity factor ( ... cleavage and polyadenylation specificity factor; TLS, translocated liposarcoma protein; LC/MS/MS, liquid chromatography-tandem ... Ruegsegger, U., Blank, D., and Keller, W. (1998) Human pre-mRNA cleavage factor Im is related to spliceosomal SR proteins and ... The purification of the cleavage- and polyadenylation-specific complex by SYM10 demonstrates that a protein within the complex ...
mRNA cleavage and polyadenylation specificity factor complex. PIP5K1A. IDA. Human. PMID:18305108. Cellular Component. GO: ...
Pre-mRNA binding subunit of the mRNA cleavage and polyadenylation specificity factor complex; involved in mRNA polyadenylation ... Essential conserved subunit of CPF cleavage and polyadenylation factor; plays a role in 3 end formation of mRNA via the ... pre-mRNA cleavage required for polyadenylation, and protein ubiquitination; localizes to both nucleus and cytosol. View ... specific cleavage and polyadenylation of pre-mRNA; contains a ubiquitin-like (UBL) domain, a RNA-binding zinc knuckle motif and ...
Cleavage and polyadenylation specificity factor 7. *Cleavage and polyadenylation specificity factor subunit 7 ... Component of the cleavage factor Im complex (CFIm) that plays a key role in pre-mRNA 3 processing. Binds to cleavage and ... Cleavage and polyadenylation specificity factor 59 kDa subunit. * ... Pre mRNA cleavage factor Im 59 kDa subunit. *Pre-mRNA cleavage ...
2012) HIV-1 capsid-targeting domain of cleavage and polyadenylation specificity factor 6. J Virol 86:3851-3860. ... restriction factors. The search for effective HIV-1 treatments and vaccines has been hampered by the lack of an animal model ... siRNA-mediated knockdown of cellular factors implicated in HIV-1 nuclear import in HeLa cells revealed that infection by most ... To replicate in a new host, lentiviruses must adapt to exploit required host factors and evade species-specific antiviral ...
Factor Interacting With PAPOLA And CPSF1, including: function, proteins, disorders, pathways, orthologs, and expression. ... Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3-end ... Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3-end ... Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and ...
Cleavage And Polyadenylation Specific Factor 4, including: function, proteins, disorders, pathways, orthologs, and expression. ... Cleavage and polyadenylation specificity factor subunit 4 Protein Accession:. O95639. Secondary Accessions: *D6W5S8 ... mRNA cleavage and polyadenylation specificity factor complex. IDA. 21102410. GO:0043231. intracellular membrane-bounded ... Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3-end ...
HIV-1 capsid-targeting domain of cleavage and polyadenylation specificity factor 6. ... human immunodeficiency virus type 1 capsid and cellular factors nucleoporin 153 and LEDGF/p75 on the efficiency and specificity ... Structural basis for nuclear import of splicing factors by human Transportin 3. ...
This interaction is mediated by the N-terminal 271 amino acids of Symplekin; CCC factors CPSF73 and CPSF100 do not contact Dcr2 ... a component of the core cleavage complex (CCC) required for 3 end processing of all eukaryotic mRNAs. ... CPSF73 or Symplekin results in nuclear retention of both hairpin and retrotransposon precursors suggesting that polyadenylation ... We have identified a nucleus-specific interaction between the essential esiRNA cleavage enzyme Dicer2 (Dcr2) and Symplekin, ...
Cleavage and polyadenylation specificity factor 1 (Hube et al., 2017); cRNA, coding RNA; ncRNA, non-coding RNA. NM_ and NR_ are ...
Cleavage and polyadenylation specificity factor 160 kDa subunit antibody. *Cleavage and polyadenylation specificity factor ... Cleavage and polyadenylation specificity factor subunit 1) is a component of the cleavage and polyadenylation specificity ... Yu S et al. SWI/SNF interacts with cleavage and polyadenylation factors and facilitates pre-mRNA 3 end processing. Nucleic ... polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step ...
cleavage and polyadenylation specificity factor subunit 4 [Source:RefSeq peptide;Acc:NP_571084]. Human Orthologue:. CPSF4. ... cleavage and polyadenylation specific factor 4, 30kDa [Source:HGNC Symbol;Acc:2327]. Mouse Orthologue:. Cpsf4. Mouse ... cleavage and polyadenylation specific factor 4 Gene [Source:MGI Symbol;Acc:MGI:1861602]. ...
Cleavage and polyadenylation specific factor 2. NM_016856. Gene Info. Cpsf3. Cleavage and polyadenylation specificity factor 3 ... Cleavage and polyadenylation specific factor 6. NM_001013391. Gene Info. Cstf2t. Cleavage stimulation factor, 3 pre-RNA ... Cleavage and polyadenylation specific factor 1. NM_053193. NM_001164173. Gene Info. ... Paf1, RNA polymerase II associated factor, homolog (S. cerevisiae). NM_019458. Gene Info. ...
nucleus • nucleoplasm • nucleolus • mRNA cleavage and polyadenylation specificity factor complex • cleavage body Biological ... Cleavage. Cleavage may refer to:. *Cleavage (breasts), partial exposure of the separation between a womans breasts. *Cleavage ... Cleavage (geology) - Types of Cleavage. ... determines what type of cleavage forms ... Cleavage is categorized as either ... The protein encoded by this gene is one subunit of a cleavage factor required for 3 RNA cleavage and polyadenylation ...
Cleavage and polyadenylation specificity factor subunit 5. O43809. C. NUMA1. Nuclear mitotic apparatus protein 1. Q14980. C. ... Platelet-activating factor acetyl hydrolase IB subunit alpha. P43034. C&T. [i] C, control sample; T, treated sample; C&T, both ... The results confirmed that the pathophysiological factors of type 2 diabetes cause centrosome amplification. ... indicating that other factors are involved. Therefore, the aim of the present study was to investigate ROCK1 binding proteins ...
Cleavage and polyadenylation specificity factor. Required for 3′ processing, splicing, and transcriptional termination of mRNAs ... mRNA splicing: splicing factor 3B subunit 1. U2-snRNP associated splicing factor forms extensive associations with the branch ... Translation termination factor. Subunit of the heterodimeric translation release factor complex involved in the release of ... Zinc-regulated transcription factor. In C. elegans, LIN-13 is involved in the tumor suppressor Rb-mediated transcriptional ...
  • CPSF-160 is the largest subunit of CPSF and directly binds to the AAUAAA polyadenylation signal. (wikipedia.org)
  • The protein encoded by this gene represents the 59 kDa subunit, which can interact with the splicing factor U2 snRNP Auxiliary Factor (U2AF) 65 to link the splicing and polyadenylation complexes. (nih.gov)
  • This gene encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. (genecards.org)
  • The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. (genecards.org)
  • In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. (genecards.org)
  • In this process, we detected a gene encoding cleavage stimulation factor, 3′ pre-RNA, subunit 2, 64 kDa (CSTF2) as a candidate. (aacrjournals.org)
  • Because there is a significant correlation of cleavage stimulation factor, 3′ pre-RNA, subunit 2, 64 kDa (CSTF2) expression with poor prognosis for patients with lung cancers, CSTF2 positivity in resected specimens could be an index that provides useful information to physicians in applying adjuvant therapy and intensive follow-up to the cancer patients who are likely to relapse. (aacrjournals.org)
  • This systematic approach revealed that cleavage stimulation factor, 3′ pre-RNA, subunit 2, 64 kDa ( CSTF2 ) gene was frequently transactivated in the majority of primary lung cancers. (aacrjournals.org)
  • The encoded protein is a 73kDa subunit of the cleavage and polyadenylation specificity factor and functions as an endonuclease that recognizes the pre-mRNA 3'-cleavage site AAUAAA prior to polyadenylation. (genscript.com)
  • CPSF3 encodes the 73-kD subunit of the cleavage and polyadenylation specificity factor (CPSF) and is the pre-mRNA 3-prime-end-processing endonuclease (Mandel et al. (thermofisher.com)
  • This kinase phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (elF-2 alpha), leading to a decrease in the rate of initiation of translation. (wikipedia.org)
  • The Arabidopsis thaliana ortholog of the 30-kD subunit of the mammalian Cleavage and Polyadenylation Specificity Factor (CPSF30) has been implicated in the responses of plants to oxidative stress, suggesting a role for alternative polyadenylation. (plantcell.org)
  • Of these subunits, one of the more enigmatic is the 30-kD subunit of the Cleavage and Polyadenylation Stimulatory Factor (termed in this report as CPSF30). (plantcell.org)
  • Our results suggest that the cleavage and polyadenylation specificity factor subunit 2 (Cpsf2), the peptidyl-prolyl cis-trans isomerase (cyclophilin)-like 2 protein (Ppil2), and the synaptosome-associated protein of 25 kDa (SNAP-25) are the most promising targets for the NSm protein of the virus during an infection. (biomedcentral.com)
  • Another protein containing this domain is the human splicing factor U2AF 35kDa subunit, which plays a critical role in both constitutive and enhancer-dependent splicing by mediating essential protein-protein interactions and protein-RNA interactions required for 3' splice site selection. (embl.de)
  • The Arabidopsis ( Arabidopsis thaliana ) gene that encodes the probable ortholog of the 30-kD subunit of the mammalian cleavage and polyadenylation specificity factor (CPSF) is a complex one, encoding small (approximately 28 kD) and large (approximately 68 kD) polypeptides. (plantphysiol.org)
  • The polyadenylation of messenger RNAs is mediated by a multi-subunit complex that is conserved in eukaryotes. (nih.gov)
  • Among the most interesting of these proteins is the 30-kDa-subunit of the Cleavage and Polyadenylation Specificity Factor, or CPSF30. (nih.gov)
  • The N-terminal domain of another Arabidopsis polyadenylation factor subunit, AtFip1(V), dramatically inhibits the nuclease activity of AtCPSF30 but has a slight negative effect on the RNA-binding activity of the protein. (nih.gov)
  • This work explores the role of cleavage and polyadenylation specificity factor subunit 6 (CPSF6) in the ability of TNPO3-depleted cells to inhibit HIV-1 infection. (pasteur.fr)
  • Structural, biochemical, and proteomic analyses of a four-subunit core module of the cleavage and polyadenylation specificity factor complex reveal its molecular architecture and specific determinants of polyadenylation signal recognition in human mRNAs. (elifesciences.org)
  • yet, the polyadenylation activity of TRF4-2, a putative human TRAMP subunit, appears to be dispensable for PABPN1-dependent regulation. (prolekare.cz)
  • Cleavage and polyadenylation specificity factor (CPSF) is involved in the cleavage of the 3' signaling region from a newly synthesized pre-messenger RNA (pre-mRNA) molecule in the process of gene transcription. (wikipedia.org)
  • CPSF-73 is a zinc-dependent hydrolase which cleaves the mRNA precursor just downstream the polyadenylation signal sequence AAUAAA. (wikipedia.org)
  • Identified proteins that are coordinated by CPSF activity include: cleavage stimulatory factor and the two poorly understood cleavage factors. (wikipedia.org)
  • We classified the archaeal homologs of this superfamily based on sequence similarity and characterized a subfamily of the Cleavage and Polyadenylation Specificity Factor (CPSF) with an uncommon domain composition: in addition to an extended MβL domain, which accommodates the active site for RNA cleavage, this group has two N-terminal KH domains. (rcsb.org)
  • Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. (genecards.org)
  • Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. (genecards.org)
  • The FIP1L1 gene provides instructions for making part of a protein complex named cleavage and polyadenylation specificity factor (CPSF). (medlineplus.gov)
  • This is the binding site for the 160-kDa component of the cleavage and PA specificity factor (CPSF) ( 17 , 30 ). (asm.org)
  • These DSEs are the binding sites for the 64-kDa component of the cleavage stimulatory factor (CStF), a complex of three proteins which interacts with the CPSF. (asm.org)
  • The poly A polymerase Star-PAP controls 3'-end cleavage by promoting CPSF interaction and specificity toward the pre-mRNA. (genscript.com)
  • The tumor suppressor Cdc73 functionally associates with CPSF and CstF 3' mRNA processing factors. (genscript.com)
  • The data support a model where Star-PAP binds to the pre-mRNA, recruits the CPSF complex to the 3'-end of pre-mRNA and then defines cleavage by CPSF 73 and subsequent polyadenylation of its target mRNAs. (genscript.com)
  • CPSF-160 recognizes the polyadenylation signal AAUAAA ( 23 ). (asm.org)
  • For example, CPSF was detected associated with transcription factor TFIID and in the RNA polymerase II (Pol II) holoenzyme, which revealed a link between transcription initiation and elongation by Pol II and processing of the 3′ end of the mRNA ( 8 , 21 ). (asm.org)
  • The Cleavage and Polyadenylation Specificity Factor (CPSF) is a multi-protein complex, crucial for both 3' processing reactions. (uni-halle.de)
  • We want to express, purify and characterise all known, as well as putative subunits of mammalian CPSF and identify the proteins that provide the functions of CPSF in cleavage and polyadenylation of eukaryotic mRNA-precursors. (uni-halle.de)
  • The 3′-end processing machinery includes the highly conserved cleavage and polyadenylation factor (CPF in yeast, CPSF in metazoans) (table S1). (sciencemag.org)
  • The specificity and efficiency of this process are determined by the binding of two core multi-protein complexes (CPSF and CSTF, for "cleavage and polyadenylation specificity factor" and "cleavage stimulation factor") to sequences surrounding the poly(A) site as soon as the nascent pre-mRNA transcript emerges from the elongating RNA polymerase II (POL2). (springer.com)
  • NS1 protein is also involved in inhibiting 3′ end processing of host mRNA by binding to CPSF 30 (cleavage and polyadenylation specificity factor 30) and PABPN1 (poly(A) binding protein nuclear 1) [ 9 ]. (hindawi.com)
  • The 3'-end-processing factor CPSF is required for the splicing of single-intron pre-mRNAs in vivo. (wikipathways.org)
  • Bridging of CPSF and CstF factors across the poly(A) site to form a stable ternary complex on the pre‐mRNA is achieved through interactions of CstF‐77 with CPSF‐160 ( Murthy and Manley, 1995 ) and other CstF subunits ( Takagaki and Manley, 1994 ). (embopress.org)
  • Cleavage factor Im (CFIm) is one of six factors necessary for correct cleavage and polyadenylation of pre-mRNAs. (nih.gov)
  • We have identified a nucleus-specific interaction between the essential esiRNA cleavage enzyme Dicer2 (Dcr2) and Symplekin, a component of the core cleavage complex (CCC) required for 3' end processing of all eukaryotic mRNAs. (biomedcentral.com)
  • Polyadenylation (PA) is the process by which the 3′ ends of most mammalian mRNAs are formed. (asm.org)
  • CstF-64 binds the GU-rich region found just downstream of the cleavage site in many pre-mRNAs ( 20 , 32 , 34 ). (asm.org)
  • The "short-form" mRNAs (PAP III, V, and VI) are produced by competition between polyadenylation and splicing, and the proteins they would produce (which have to date not been detected) would be truncated in the middle of the catalytic region ( 40 ). (asm.org)
  • end cleavage and polyadenylation of host pre-mRNAs. (avivasysbio.com)
  • Most eukaryotic mRNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3-prime end. (thermofisher.com)
  • It prevents polyadenylation of cellular mRNAs to circumvent antiviral responses of the host, e.g., maturation and translation of interferon mRNAs. (wikipedia.org)
  • Cis-acting regulatory sequence elements are sequences contained in the 3′ and 5′ untranslated region, introns, or coding regions of precursor RNAs and mature mRNAs that are selectively recognized by a complementary set of one or more trans-acting factors to regulate posttranscriptional gene expression. (intechopen.com)
  • Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3′ ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). (sciencemag.org)
  • We report the recovery and analysis of the grechetto mutant, which harbors an inactivating mutation in cleavage and polyadenylation specificity factor 1 (cpsf1), a gene ubiquitously expressed and required for 3' UTR processing of a subset of pre-mRNAs. (zfin.org)
  • Alternative processing of pre-mRNAs is an important factor in sculpting the proteome of eukaryotes, including plants. (plantphysiol.org)
  • CPEB determines polyadenylation status, and consequently translational efficiency, of its target mRNAs in both transcriptionally active somatic cells as well as in transcriptionally silent mature Xenopus oocytes and early embryos. (royalsocietypublishing.org)
  • Influenza virus inhibits cleavage of the HSP70 pre-mRNAs at the polyadenylation site. (wikipathways.org)
  • This process, which consists in the recognition of defined poly(A) signals of the pre-mRNAs by a large cleavage/polyadenylation machinery, plays a critical role in gene expression. (pubmedcentralcanada.ca)
  • After cleavage, most pre-mRNAs, with the exception of histone replication-dependent transcripts, acquire a polyadenylated tail. (pubmedcentralcanada.ca)
  • Instead, U‐rich elements immediately upstream and downstream of the poly(A) site mediate cleavage‐site recognition within CYC1 and ADH1 pre‐mRNAs. (embopress.org)
  • Biochemical assays and a cocrystal structure of the benzoxabarole bound to the active site of parasitic cleavage and polyadenylation specificity factor 3 (CPSF3) suggested that the selective targeting of Cryptosporidium by the benzoxabarole resulted from inhibition of parasitic pre-mRNA processing. (sciencemag.org)
  • Cleavage stimulation factor (CstF) is required for efficient cleavage. (asm.org)
  • This reflects an increase in intact CstF, which results in enhanced usage of a weak upstream polyadenylation signal, which in turn enhances synthesis of the secreted-form IgM mRNA ( 33 ). (asm.org)
  • Quiescent fibroblasts contain reduced levels of the cleavage and polyadenylation factor CstF-64. (biomedcentral.com)
  • The AAUAAA sequence is located between 11 and 25 nucleotides upstream of the actual cleavage and PA site. (asm.org)
  • Cleavage factors I and/or II (CF I and CF II [ 30 ]) are likely directly involved in cleavage of the pre-mRNA, which occurs about 10 to 30 nucleotides downstream of AAUAAA. (asm.org)
  • Cytoplasmic polyadenylation element binding protein 3 (CPEB3) is a sequence-specific RNA-binding protein that confines the strength of glutamatergic synapses by translationally downregulating the expression of multiple plasticity-related proteins (PRPs), including the N -methyl-D-aspartate receptor (NMDAR) and the postsynaptic density protein 95 (PSD95). (frontiersin.org)
  • Recent research highlights the significance of the cytoplasmic polyadenylation element-binding protein (CPEB). (royalsocietypublishing.org)
  • Cytoplasmic polyadenylation element binding proteins in development, health, and disease. (umassmed.edu)
  • FIP1L1 (Factor Interacting With PAPOLA And CPSF1) is a Protein Coding gene. (genecards.org)
  • Consistent with Cpsf1 function, c-myb:EGFP+ cells in grechetto mutants also show defective polyadenylation of snrnp70, a gene required for HSC development. (zfin.org)
  • There are two coupled reactions in polyadenylation, the endonucleolytic cleavage of the pre-mRNA and the synthesis of the poly(A) tail onto the cleaved mRNA. (asm.org)
  • To replicate in a new host, lentiviruses must adapt to exploit required host factors and evade species-specific antiviral proteins. (pnas.org)
  • Proteins containing CCCH Znf domains include Znf proteins from eukaryotes involved in cell cycle or growth phase-related regulation, e.g. human TIS11B (butyrate response factor 1), a probable regulatory protein involved in regulating the response to growth factors, and the mouse TTP growth factor-inducible nuclear protein, which has the same function. (embl.de)
  • Tristetraprolin (TTP), the prototype of a class of CCCH zinc finger proteins, is a phosphoprotein that is rapidly and transiently induced by growth factors and serum in fibroblasts. (embl.de)
  • By protein sequence homology search using human and yeast polyadenylation factors, we identified 28 proteins that may be components of Arabidopsis polyadenylation machinery. (biomedcentral.com)
  • An extensive protein network was revealed for plant polyadenylation machinery, in which all predicted proteins were found to be connecting to the complex. (biomedcentral.com)
  • MOV10 also interacts with ISG, IRAV (FLJ11286), and depletion of either factor during dengue virus infection resulted in increased levels of viral mRNA, suggesting that these proteins may function together to de-stabilize viral RNA [ 7 ]. (biochemj.org)
  • CCC factors CPSF73 and CPSF100 do not contact Dcr2. (biomedcentral.com)
  • RNAi-depletion of either CPSF73 or Symplekin results in nuclear retention of both hairpin and retrotransposon precursors suggesting that polyadenylation indirectly affects cellular localization of Dcr2 substrates. (biomedcentral.com)
  • A complex containing the CPSF73 endonuclease and other polyadenylation factors associates with U7 snRNP and is recruited to histone pre-mRNA for 3'-end processing. (genscript.com)
  • A metallo β-lactamase (MBL) fold enzyme, cleavage and polyadenylation specificity factor 73 (CPSF73), is proposed to be the sole endonuclease responsible for 3' end processing of both mRNA classes. (babraham.ac.uk)
  • The vast majority of all eukaryotic mRNA-precursors are 3' processed by cleavage and polyadenylation. (uni-halle.de)
  • The eukaryotic mRNA 3′-end processing machinery interacts with the transcription machinery and adds the polyadenylation tail on the mRNA substrate. (sciencemag.org)
  • The polyadenylation of mRNA is one of the critical processing steps during expression of almost all eukaryotic genes. (biomedcentral.com)
  • Messenger RNA polyadenylation is one of the critical steps in eukaryotic gene expression. (westernu.edu)
  • Here, we review the eukaryotic 3′ end processing machineries as well as the comprehensive set of regulatory factors and discuss the different molecular mechanisms of 3′ end processing regulation by proposing several overlapping models of regulation. (pubmedcentralcanada.ca)
  • It is the first protein to bind to the signaling region near the cleavage site of the pre-mRNA, to which the poly(A) tail will be added by polynucleotide adenylyltransferase. (wikipedia.org)
  • This gene, the homolog of yeast Fip1 (factor interacting with PAP), binds to U-rich sequences of pre-mRNA and stimulates poly(A) polymerase activity. (genecards.org)
  • Since the discovery of poly(A) polymerase (PAP) almost 40 years ago ( 9 ), much progress has been made toward the understanding of the function of poly(A) tails, as well as the machinery that carries out polyadenylation (for reviews, see references 6 and 37 ). (asm.org)
  • Cleavage and polyadenylation specificity factor is required for both the cleavage and the poly(A) synthesis phases of the reaction. (asm.org)
  • Using a tandem affinity purification tag, we purified native CPF from yeast ( Fig. 1A ) that was active and specific in cleavage and polyadenylation assays: CPF cleaves a model CYC1 pre-mRNA in vitro and adds a poly(A) tail onto the 5′ (but not 3′) cleavage product ( Fig. 1B and fig. S1). (sciencemag.org)
  • The polyadenylation process requires two components, the cis -elements on the mRNA and a group of protein factors that recognize the cis -elements and produce the poly(A) tail. (biomedcentral.com)
  • This apparatus consists of a complex of factors that control the action of poly(A) polymerases, limiting polyadenylation to RNAs containing polyadenylation signals. (biomedcentral.com)
  • It has been demonstrated in recent years that alternative polyadenylation, a process that generates a few poly(A) sites on different transcripts of a gene, is a widely used post-transcriptional molecular mechanism in gene expression regulation, including cancer development, embryo development and stress responses. (westernu.edu)
  • We are using bioinformatics tools to search and analyze such polyadenylation signals in the plant genomes, and building models and algorithms to predict poly(A) sites. (westernu.edu)
  • In eukaryotes, 3′ end cleavage of transcripts generated by RNA polymerase II (pol II) is a universal step of gene expression that proceeds through the recognition of cis -acting elements of the pre-messenger RNA (mRNA) [defined as the poly(A) signal] by a complex machinery. (pubmedcentralcanada.ca)
  • Indeed, 3′ end polyadenylation factors (or pA factors, including factors involved in both cleavage and polyadenylation) and sequence elements of the poly(A) signal modulate transcription termination ( 2-5 ) and, in turn, transcription factors/activators affect processing at the poly(A) signal ( 6-9 ). (pubmedcentralcanada.ca)
  • The functional interdependence between splicing and 3′ end processing is mediated by the molecular link between splicing factors bound at the last intron 3′ splice site and pA factors associated to the poly(A) signal in the terminal exon [( 12-16 ) and references inside] and contributes to define the last exon of a pre-mRNA ( 17 ). (pubmedcentralcanada.ca)
  • The product of the PABPN1 gene was originally identified as a factor that stimulates the synthesis of RNA poly(A) tails in vitro [1] . (prolekare.cz)
  • Consistent with a role in polyadenylation, siRNA-mediated gene silencing experiments in primary mouse myoblasts showed global shortening of mRNA poly(A) tails after PABPN1 depletion [6] . (prolekare.cz)
  • Employing an in vitro cleavage system with cleavage and polyadenylation factor (CPF) and cleavage factor IA we show that the efficiency and positioning elements are dispensable for poly(A)‐site recognition within a short CYC1 substrate in vitro . (embopress.org)
  • These elements act in concert with the poly(A) site to produce multiple recognition sites for the processing machinery, since combinations of mutations within these elements were most effective in cleavage inhibition. (embopress.org)
  • Intriguingly, introduction of a U‐rich element downstream of the GAL7 poly(A) site strongly enhanced cleavage, underscoring the importance of downstream sequences in general. (embopress.org)
  • RNA‐ binding analyses demonstrate that cleavage depends on the recognition of the poly(A)‐site region by CPF. (embopress.org)
  • Specific polyadenylation occurs when CF IA, CF IB, poly(A)‐binding protein (Pab1p) and polyadenylation factor I (PF I) are present. (embopress.org)
  • S., Wahle, E. (2009) Poly(A) tail length is controlled by the nuclear poly(A) binding protein regulating the interaction between poly(A) polymerase and the cleavage and polyadenylation specificity factor. (uni-wuerzburg.de)
  • Presumably, the efficiency of polyadenylation of male-specific transcripts, which contain noncanonical polyadenylation signals, is determined by overall levels of the male-specific CstF64 isoform. (plantphysiol.org)
  • Factors that are involved in directing the cleavage and POLYADENYLATION of the of MESSENGER RNA near the site of the RNA 3' POLYADENYLATION SIGNALS. (umassmed.edu)
  • RNA sequencing of fibroblasts induced into quiescence by contact inhibition reveals downregulation of genes involved in mRNA processing, including splicing and cleavage and polyadenylation factors. (biomedcentral.com)
  • An interstitial chromosomal deletion on 4q12 creates an in-frame fusion of human genes FIP1L1 and PDGFRA (platelet-derived growth factor receptor, alpha). (genecards.org)
  • Surprisingly, depletion of the ubiquitous Pol-II-associated transcription elongation factor SPT6 promotes a redistribution of H3K36me3 histone marks from active protein coding to lncRNA genes, which correlates with increased lncRNA transcription. (babraham.ac.uk)
  • When comparing the gene expression profiles extracted from publicly available microarray datasets, some of the polyadenylation related genes showed tissue-specific expression, suggestive of potential different polyadenylation complex configurations. (biomedcentral.com)
  • These results offer a roadmap for further functional characterizations of the protein factors, and for building models when testing the genetic contributions of these genes in plant growth and development. (biomedcentral.com)
  • In particular, alternative polyadenylation of certain genes plays an important role in many biological processes like flower development, self-incompatibility, stress responses, etc. (westernu.edu)
  • We have used bioinformatics and molecular biology approaches to identify and clone plant (Arabidopsis) polyadenylation related genes . (westernu.edu)
  • The emergent theme is that over 70% genes use alternative polyadenylation to regulate their expression. (westernu.edu)
  • In fission yeast, a genome-wide analysis of gene expression changes in cells deleted for pab2 , which encodes the PABPN1 ortholog, revealed that the expression of most protein-coding genes was unaffected, indicating that Pab2 does not function as a general mRNA maturation factor in this organism [8] . (prolekare.cz)
  • At the effector domain, it interacts and inhibits cleavage and polyadenylation specificity factor (CPSF30). (wikipedia.org)
  • E7 oncoprotein binds to pRb tumor-suppressor family[ 5 , 6 ], and interacts with transcription factor EZH2, a member of the Polycomb Repressive Complex 2 (PRC2) that involves in S phase of cell cycle[ 7 ]. (jcancer.org)
  • In alliance with matrix protein 1(M1), it interacts with cellular export factor (CEF1) and modulates nuclear export of viral ribonucleoprotein (vRNP) complexes by connecting cellular export machinery with vRNPs 14 . (ukessays.com)
  • Taken together, these studies provide new insights into the mechanisms and consequences of CPSF30-mediated alternative polyadenylation. (plantcell.org)
  • The polymerase module of the complex acts as a hub to bring the RNA substrate and the accessory factors together to achieve efficient and controlled polyadenylation coordinated with transcription. (sciencemag.org)
  • It should be noted that the RNAs labeled by [32P]-pCp in lane 3 of Figure 2 do not correspond in size to those expected if cleavage was occurring at the three 'natural' polyadenylation sites in the substrate RNA (the latter would be expected to yield 5′ cleavage products of 125, 145 and 175 nt) (32,35). (nih.gov)
  • This processing is directed by distinct polyadenylation signal sequences present in the substrate RNAs. (biomedcentral.com)
  • The anticodon is thus a major determinant for tadA substrate specificity. (labome.org)
  • The polyadenylation machinery is composed of multiple factors. (asm.org)
  • The cloning of components of the polyadenylation machinery has facilitated an understanding of cellular regulation of pre-mRNA processing. (asm.org)
  • Here we report a comprehensive pairwise protein-protein interaction mapping and gene expression profiling of the mRNA polyadenylation protein machinery in Arabidopsis. (biomedcentral.com)
  • A large number of protein factors associates with this machinery to regulate the efficiency and specificity of this process and to mediate its interaction with other nuclear events. (pubmedcentralcanada.ca)
  • In yeast, 3'-end formation is mediated by a complex that also consists of several factors, each of which in turn consists of several polypeptide subunits. (biomedcentral.com)
  • Stable and stoichiometric association of CF II and PF I subunits in CPF suggest that this factor forms a functional unit in vivo . (embopress.org)
  • A second downstream signaling region, located on the portion of the pre-mRNA that is cleaved before polyadenylation, consists of a GU-rich region required for efficient processing. (wikipedia.org)
  • the finding of robust association to SLE of SNPs in IRF5 , a transcription factor downstream of the type I IFN and Toll-like receptors ( 7 - 10 ), provided the first direct and specific evidence that variation in the type I IFN pathway plays a causal role in SLE pathogenesis ( 5 , 6 ). (pnas.org)
  • These macroevolutionary changes in function between Tribolium and Drosophila are accompanied by regulatory network changes, where BMP signaling and possibly the transcription factor Hindsight are downstream mediators. (sdbonline.org)
  • Combined with in vitro reconstitution experiments, our data show that the polymerase module brings together factors required for specific and efficient polyadenylation, to help coordinate mRNA 3′-end processing. (sciencemag.org)
  • These interactions organize into three distinct hubs involving the Arabidopsis polyadenylation factors. (biomedcentral.com)
  • Although cleavage and polyadenylation can be studied as isolated processes in vitro , mRNA 3′ end formation in vivo is an integral component of the coupled network in which the different machines carrying out separate steps of the gene expression pathway are tethered to each other to form a gene expression factory. (pubmedcentralcanada.ca)
  • Protein factors can be separated biochemically that are sufficient to reconstitute cleavage and polyadenylation in vitro ( Chen and Moore, 1992 ). (embopress.org)
  • CPSF4 (Cleavage And Polyadenylation Specific Factor 4) is a Protein Coding gene. (genecards.org)
  • Muscle identity reflects the expression by each PC of a specific combination of identity Transcription Factors (iTFs). (sdbonline.org)
  • An important goal of influenza A virus (IAV) research is to identify specific host factors that can limit or block particular steps in the virus replication cycle. (biochemj.org)
  • A tissue-specific transcription factor is involved in the regulation of alternative polyadenylation in conjunction with a transcription termination factor. (elifesciences.org)
  • Site‐specific cleavage requires cleavage and polyadenylation factor IA (CF IA), cleavage and polyadenylation factor IB (CF IB) and cleavage factor II (CF II). (embopress.org)
  • In this network, 3′ end processing cross-talks with the transcription and splicing steps to optimize the efficiency and specificity of each enzymatic reaction ( Figure 1 ) (1). (pubmedcentralcanada.ca)
  • One unifying concept states that posttranscriptional regulation is mediated by two major molecular components: cis-acting regulatory sequence elements and trans-acting factors. (intechopen.com)
  • These results show that the central portion of AtCPSF30 is involved in a number of important functions, and they raise interesting possibilities for both the interplay between splicing and polyadenylation and the regulation of these processes by stimuli that act through calmodulin. (plantphysiol.org)
  • In recent years, it has been demonstrated that the polyadenylation process is also involved in the regulation of gene expression. (biomedcentral.com)
  • In plants, it has been shown that the polyadenylation process, and the alternative polyadenylation of mRNA, can lead to gene expression regulation. (westernu.edu)
  • This includes transcriptome level analysis of gene expression regulation through post-transcriptional means like mRNA polyadenylation and splicing. (westernu.edu)
  • Binds to cleavage and polyadenylation RNA substrates. (nih.gov)
  • Evidence that cleavage factor Im is a heterotetrameric protein complex controlling alternative polyadenylation. (nih.gov)
  • However, no attempt has been so far made to map the RNA-binding sites along the protein sequence and to identify possible differential RNA-sequence specificity. (embl-heidelberg.de)
  • In addition to changes in mRNA expression, the sequences of many transcripts are altered by alternative splicing and alternative polyadenylation (APA). (jimmunol.org)
  • In cell biology, the Cleavage furrow is the indentation that begins the process of cleavage, by which animal cells undergo cytokinesis. (primidi.com)
  • I am interested to elucidate plant mRNA polyadenylation mechanisms at genetic, biochemical, and molecular levels. (westernu.edu)
  • Beyond the links with mRNA synthesis, transport, and function, polyadenylation also affects gene expression by determining the coding and regulatory potential of an mRNA. (plantcell.org)
  • Recent illustrations include the role of the complement pathway in age-related macular degeneration ( 1 - 3 ), the IL-23 pathway in inflammatory bowel disease ( 4 ), and IFN regulatory factor 5 ( IRF5 ) in systemic lupus erythematosus (SLE) ( 5 , 6 ). (pnas.org)
  • Background: Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). (cellsignal.com)
  • A model for yeast pre‐mRNA cleavage‐site recognition outlines an unanticipated high conservation of yeast and mammalian 3′‐end processing mechanisms. (embopress.org)
  • complex assembly and cleavage site selection in yeast are believed to differ significantly from mammals. (embopress.org)
  • Longer transcripts that end at distal polyadenylation sites tend to be more stable than shorter transcripts generated from proximal polyadenylation site use in proliferating cells. (biomedcentral.com)
  • NS1 could be divided into two functional domains: (i) N-terminal RNA binding domain (residues 1-73) and (ii) C-terminal effector domain, interacting with several host factors (residues 74-230) [ 3 - 6 ]. (hindawi.com)
  • The study of a recurrent breast cancer MAGI3 truncation reveals the role of MAGI3 in regulating the Hippo effector YAP and highlights premature mRNA cleavage and polyadenylation as a mechanism underlying cancer development. (elifesciences.org)
  • The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. (antikoerper-online.de)
  • Regulated polyadenylation in mammals is affected by a number of different mechanisms. (plantphysiol.org)
  • Interestingly, while both subfamilies potently decrease the population of cellular mRNA, the viral host shutoff factors and their mechanisms of action are distinct. (asm.org)
  • Despite significant progress in understanding the mechanisms by which some restriction factors mediate anti-IAV activity, many questions remain to be answered. (biochemj.org)
  • Of those identified to date, the mechanisms underlying the anti-IAV activity of many restriction factors are still poorly defined. (biochemj.org)
  • Understanding mechanisms governing the expression and the anti-IAV activity of different restriction factors is essential to define their role in antiviral immunity and to allow for studies which can assess their potential as targets for the development of novel antiviral treatments. (biochemj.org)
  • Using the spliced transcript produced from a snoRNA host gene as a model lncRNA, we show that PABPN1 promotes lncRNA turnover via a polyadenylation-dependent mechanism. (prolekare.cz)
  • The proposal aims to identify the factors responsible for the reaction by an affinity purification approach with the goal of analyzing the mechanism of accelerated deadenylation. (uni-wuerzburg.de)
  • Background: The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). (cellsignal.com)
  • Finally and most intriguingly, we will discuss to what extent alternative cleavage and polyadenylation represent the driver or passenger in the pathogenesis of human disorders. (springer.com)
  • However, more dramatic instances of alternative polyadenylation in plants have been described. (plantphysiol.org)
  • We are performing large-scale analyses of alternative polyadenylation to discover the full landscape of polyadenylation in plants. (westernu.edu)
  • 2018. APAtrap: identification and quantification of alternative polyadenylation sites from RNA-seq data. (westernu.edu)
  • 2018. Alternative polyadenylation is involved in auxin-based plant growth and development. (westernu.edu)