Fluid accumulation in the outer layer of the MACULA LUTEA that results from intraocular or systemic insults. It may develop in a diffuse pattern where the macula appears thickened or it may acquire the characteristic petaloid appearance referred to as cystoid macular edema. Although macular edema may be associated with various underlying conditions, it is most commonly seen following intraocular surgery, venous occlusive disease, DIABETIC RETINOPATHY, and posterior segment inflammatory disease. (From Survey of Ophthalmology 2004; 49(5) 470-90)
Messages between computer users via COMPUTER COMMUNICATION NETWORKS. This feature duplicates most of the features of paper mail, such as forwarding, multiple copies, and attachments of images and other file types, but with a speed advantage. The term also refers to an individual message sent in this way.
A pathological process characterized by injury or destruction of tissues caused by a variety of cytologic and chemical reactions. It is usually manifested by typical signs of pain, heat, redness, swelling, and loss of function.
Disease of the RETINA as a complication of DIABETES MELLITUS. It is characterized by the progressive microvascular complications, such as ANEURYSM, interretinal EDEMA, and intraocular PATHOLOGIC NEOVASCULARIZATION.
An esterified form of TRIAMCINOLONE. It is an anti-inflammatory glucocorticoid used topically in the treatment of various skin disorders. Intralesional, intramuscular, and intra-articular injections are also administered under certain conditions.
The use of green light-producing LASERS to stop bleeding. The green light is selectively absorbed by HEMOGLOBIN, thus triggering BLOOD COAGULATION.
An imaging method using LASERS that is used for mapping subsurface structure. When a reflective site in the sample is at the same optical path length (coherence) as the reference mirror, the detector observes interference fringes.
Inflammation of the RETINA. It is rarely limited to the retina, but is commonly associated with diseases of the choroid (CHORIORETINITIS) and of the OPTIC DISK (neuroretinitis).
Abnormal intravascular leukocyte aggregation and clumping often seen in leukemia patients. The brain and lungs are the two most commonly affected organs. This acute syndrome requires aggressive cytoreductive modalities including chemotherapy and/or leukophoresis. It is differentiated from LEUKEMIC INFILTRATION which is a neoplastic process where leukemic cells invade organs.
The property of blood capillary ENDOTHELIUM that allows for the selective exchange of substances between the blood and surrounding tissues and through membranous barriers such as the BLOOD-AIR BARRIER; BLOOD-AQUEOUS BARRIER; BLOOD-BRAIN BARRIER; BLOOD-NERVE BARRIER; BLOOD-RETINAL BARRIER; and BLOOD-TESTIS BARRIER. Small lipid-soluble molecules such as carbon dioxide and oxygen move freely by diffusion. Water and water-soluble molecules cannot pass through the endothelial walls and are dependent on microscopic pores. These pores show narrow areas (TIGHT JUNCTIONS) which may limit large molecule movement.
The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.
The clear, watery fluid which fills the anterior and posterior chambers of the eye. It has a refractive index lower than the crystalline lens, which it surrounds, and is involved in the metabolism of the cornea and the crystalline lens. (Cline et al., Dictionary of Visual Science, 4th ed, p319)
The ten-layered nervous tissue membrane of the eye. It is continuous with the OPTIC NERVE and receives images of external objects and transmits visual impulses to the brain. Its outer surface is in contact with the CHOROID and the inner surface with the VITREOUS BODY. The outer-most layer is pigmented, whereas the inner nine layers are transparent.
An intermediate filament protein found only in glial cells or cells of glial origin. MW 51,000.
Cell-cell junctions that seal adjacent epithelial cells together, preventing the passage of most dissolved molecules from one side of the epithelial sheet to the other. (Alberts et al., Molecular Biology of the Cell, 2nd ed, p22)
Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue.
A claudin subtype that is found localized to TIGHT JUNCTIONS in VASCULAR ENDOTHELIAL CELLS. The protein was initially identified as one of several proteins which are deleted in VELOCARDIOFACIAL SYNDROME and may play an important role in maintaining the integrity of the BLOOD-BRAIN BARRIER.
A MARVEL domain protein that plays an important role in the formation and regulation of the TIGHT JUNCTION paracellular permeability barrier.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
A 195-kDa zonula occludens protein that is distinguished by the presence of a ZU5 domain at the C-terminal of the molecule.

Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cells. (1/124)

Tight junctions (TJs) in endothelial cells are thought to determine vascular permeability. Recently, claudin-1 to -15 were identified as major components of TJ strands. Among these, claudin-5 (also called transmembrane protein deleted in velo-cardio-facial syndrome [TMVCF]) was expressed ubiquitously, even in organs lacking epithelial tissues, suggesting the possible involvement of this claudin species in endothelial TJs. We then obtained a claudin-6-specific polyclonal antibody and a polyclonal antibody that recognized both claudin-5/TMVCF and claudin-6. In the brain and lung, immunofluorescence microscopy with these polyclonal antibodies showed that claudin-5/TMVCF was exclusively concentrated at cell-cell borders of endothelial cells of all segments of blood vessels, but not at those of epithelial cells. Immunoreplica electron microscopy revealed that claudin-5/TMVCF was a component of TJ strands. In contrast, in the kidney, the claudin-5/TMVCF signal was restricted to endothelial cells of arteries, but was undetectable in those of veins and capillaries. In addition, in all other tissues we examined, claudin-5/TMVCF was specifically detected in endothelial cells of some segments of blood vessels, but not in epithelial cells. Furthermore, when claudin-5/TMVCF cDNA was introduced into mouse L fibroblasts, TJ strands were reconstituted that resembled those in endothelial cells in vivo, i.e., the extracellular face-associated TJs. These findings indicated that claudin-5/TMVCF is an endothelial cell-specific component of TJ strands.  (+info)

Claudin promotes activation of pro-matrix metalloproteinase-2 mediated by membrane-type matrix metalloproteinases. (2/124)

Genes associated with regulation of membrane-type matrix metalloproteinase-1 (MT1-MMP)-mediated pro-MMP-2 processing were screened in 293T cells by a newly developed expression cloning method. One of the gene products, which promoted processing of pro-MMP-2 by MT1-MMP was claudin-5, a major component of endothelial tight junctions. Expression of claudin-5 not only replaced TIMP-2 in pro-MMP-2 activation by MT1-MMP but also promoted activation of pro-MMP-2 mediated by all MT-MMPs and MT1-MMP mutants lacking the transmembrane domain (DeltaMT1-MMP). A carboxyl-terminal deletion mutant of pro-MMP-2 (proDeltaMMP-2) was processed to an intermediate form by MT1-MMP in 293T cells and was further converted to an activated form by introduction of claudin-5. In contrast to the stimulatory effect of TIMP-2 on pro-MMP-2 activation by MT1-MMP, activation of pro-MMP-2 by DeltaMT1-MMP in the presence of claudin-5 and proDeltaMMP-2 processing by MT1-MMP were both inversely repressed by expression of exogenous TIMP-2. These results suggest that TIMP-2 is not involved in cluadin-5-induced pro-MMP-2 activation by MT-MMPs. Stimulation of MT-MMP-mediated pro-MMP-2 activation was also observed with other claudin family members, claudin-1, claudin-2, and claudin-3. Amino acid substitutions or deletions in ectodomain of claudin-1 abolished stimulatory effect. Direct interaction of claudin-1 with MT1-MMP and MMP-2 was demonstrated by immunoprecipitation analysis. MT1-MMP was co-localized with claudin-1 not only at cell-cell borders, but also at other parts of the cells. TIMP-2 enhanced cell surface localization of MMP-2 mediated by MT1-MMP, and claudin-1 also stimulated it. These results suggest that claudin recruits all MT-MMPs and pro-MMP-2 on the cell surface to achieve elevated focal concentrations and, consequently, enhances activation of pro-MMP-2.  (+info)

The renal segmental distribution of claudins changes with development. (3/124)

BACKGROUND: Permeability properties of mammalian nephron are tuned during postnatal maturation. The transepithelial electrical resistance (TER) and complexity of tight junctions (TJs) vary along the different tubular segments, suggesting that the molecules constituting this structure change. We studied the differential expression of occludin and several claudins in isolated renal tubules from newborn and adult rabbits. METHODS: Isolated renal tubules from newborn and adult rabbits were processed for occludin, claudin-1 and claudin-2 immunofluorescence, and Western blot detection of claudin-1 and -2. Claudin-5 was detected in whole kidney frozen sections. RT-PCR from isolated tubules was performed for claudins-1 to -8. RESULTS: Immunofluorescence revealed that occludin, claudin-1 and -2 were present at the cell boundaries at the neonatal stage of development. Claudin-1 was detected in the tighter segments of the nephron (distal and collecting duct), while claudin-2 was found in the leaky portions (proximal). Claudin 5 was found in the kidney vasculature. PCR amplification revealed the presence of claudins-1 to -4 in tubules of newborns. In adults, claudins-1, -2 and -4 were present in proximal, Henle's loop and collecting segments; claudin-3 was in proximal and collecting tubules, while claudins-5 and -6 were absent from all tubular portions. Claudin-7 was restricted to proximal tubules, while claudin-8 was present in proximal and Henle's segments. CONCLUSIONS: The pattern of occludin distribution is present from the neonatal age. Claudins-7 and -8 are up-regulated after birth. Each tubular segment expresses a peculiar set of claudins that might be responsible for the permeability properties of their TJs.  (+info)

Prolonged fluid shear stress induces a distinct set of endothelial cell genes, most specifically lung Kruppel-like factor (KLF2). (4/124)

The endothelium expresses a large repertoire of genes under apparent transcriptional control of biomechanical forces, many of which are neither cell-type nor flow specific. We set out to identify genes that are uniquely flow responsive in human vascular endothelial cells. Transcriptional profiling using commercial DNA microarrays identified 12 of 18 000 genes that were modulated at least 5-fold after 24 hours of steady laminar flow (25 dyne/cm(2)). After a 7-day exposure to unidirectional pulsatile flow (19 +/- 12 dyne/cm(2)), only 3 of 12 remained elevated at least 5-fold. A custom microarray of ~300 vascular cell-related gene fragments was constructed, and expression analysis revealed that many flow-induced genes are also induced by at least one of the following agents: tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), transforming growth factor-beta, vascular endothelial growth factor, or thrombin, indicating a more general role in adaptive or stress responses. Most flow-induced genes were also induced by TNF-alpha but not IL-1beta, suggesting the involvement of reactive oxygen species. A limited panel of genes that are unique for flow-exposed cultures was identified, including lung Kruppel-like factor (LKLF/KLF2) and cytochrome P450 1B1 (CYP1B1). In marked contrast, both these genes were substantially repressed by TNF-alpha. LKLF but not CYP1B1 mRNA was detected exclusively in the vascular endothelium of healthy human aorta by in situ hybridization and appeared to be flow regulated. To date LKLF is the first endothelial transcription factor that is uniquely induced by flow and might therefore be at the molecular basis of the physiological healthy, flow-exposed state of the endothelial cell.  (+info)

Distinct claudins and associated PDZ proteins form different autotypic tight junctions in myelinating Schwann cells. (5/124)

The apposed membranes of myelinating Schwann cells are joined by several types of junctional specializations known as autotypic or reflexive junctions. These include tight, gap, and adherens junctions, all of which are found in regions of noncompact myelin: the paranodal loops, incisures of Schmidt-Lanterman, and mesaxons. The molecular components of autotypic tight junctions have not been established. Here we report that two homologues of Discs Lost-multi PDZ domain protein (MUPP)1, and Pals-associated tight junction protein (PATJ), are differentially localized in myelinating Schwann cells and associated with different claudins. PATJ is mainly found at the paranodal loops, where it colocalized with claudin-1. MUPP1 and claudin-5 colocalized in the incisures, and the COOH-terminal region of claudin-5 interacts with MUPP1 in a PSD-95/Disc Large/zona occludens (ZO)-1 (PDZ)-dependent manner. In developing nerves, claudin-5 and MUPP1 appear together in incisures during the first postnatal week, suggesting that they coassemble during myelination. Finally, we show that the incisures also contain four other PDZ proteins that are found in epithelial tight junctions, including three membrane-associated guanylate-kinase proteins (membrane-associated guanylate-kinase inverted-2, ZO-1, and ZO-2) and the adaptor protein Par-3. The presence of these different tight junction proteins in regions of noncompact myelin may be required to maintain the intricate cytoarchitecture of myelinating Schwann cells.  (+info)

Holey barrier: claudins and the regulation of brain endothelial permeability. (6/124)

Endothelial tight junctions (TJs)* are an important functional part of the blood-brain barrier (BBB). In this issue, Nitta et al. (2003) demonstrate that claudin-5, a transmembrane protein of TJs, is a critical determinant of BBB permeability in mice. Unexpectedly, knockout of claudin-5 did not result in a general breakdown of TJs but in a selective increase in paracellular permeability of small molecules. This suggests that the BBB can be manipulated to allow selective diffusion of small molecules and makes claudin-5 a possible target for the development of drugs for this purpose.  (+info)

Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice. (7/124)

Tight junctions are well-developed between adjacent endothelial cells of blood vessels in the central nervous system, and play a central role in establishing the blood-brain barrier (BBB). Claudin-5 is a major cell adhesion molecule of tight junctions in brain endothelial cells. To examine its possible involvement in the BBB, claudin-5-deficient mice were generated. In the brains of these mice, the development and morphology of blood vessels were not altered, showing no bleeding or edema. However, tracer experiments and magnetic resonance imaging revealed that in these mice, the BBB against small molecules (<800 D), but not larger molecules, was selectively affected. This unexpected finding (i.e., the size-selective loosening of the BBB) not only provides new insight into the basic molecular physiology of BBB but also opens a new way to deliver potential drugs across the BBB into the central nervous system.  (+info)

Role of claudin interactions in airway tight junctional permeability. (8/124)

Airway epithelial tight junctions (TJs) serve to separate the external and internal environments of the lung. However, the members of the claudin family that mediate this function have not been fully delineated. We characterized the claudin expression in normal airways removed from human donors during lung transplantation and determined the contribution of each claudin to airway barrier function. Stable cell lines in NIH/3T3 and human airway (IB3.1) cells were constructed expressing the claudin components found in the human airway, claudin-1, -3, or -5. The effects of claudin expression on transepithelial resistance, permeability coefficients, and claudin-claudin interactions were assessed. Claudin-1 and -3 decreased solute permeability, whereas claudin-5 increased permeability. We also detected oligomerization of claudin-5 in cell lines and in freshly excised human airways. Coimmunoprecipitation studies revealed heterophilic interactions between claudin species in both cell lines and human airway epithelium. These suggest that airway TJs are regulated by claudinclaudin interactions that confer the selectivity of the junction.  (+info)

Hypoxic Stress Induced by Hydralazine Leads to a Loss of Blood-Brain Barrier Integrity and an Increase in Efflux Transporter Activity. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
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Impaired blood-brain barrier function represents a significant component of hypoxic-ischemic brain injury in the perinatal period. Banks, W. A., Stonestreet, B. S. AntiCIL-6 neutralizing antibody modulates blood-brain barrier function in the ovine fetus. mAb attenuate ischemia-reperfusionCrelated increases in BBB permeability in sheep fetuses (16). However, the role of IL-6 after injury in the immature brain has been studied much less extensively than those of IL-1and TNF-in the immature brain. We recently generated pharmacologic quantities of a highly selective, ovine-specific antiCIL-6 mAb and antiCIL-1mAb. The neutralizing abilities of these mAbs have previously been confirmed in ovine splenic mononuclear cell cultures (35). Moreover, we recently demonstrated that infusions of an antiCIL-1mAb result in the uptake of the antiCIL-1mAb into the brain and attenuate ischemia-reperfusionCrelated increases in BBB permeability in ovine fetal brain using the preclinical translational fetal sheep model ...
Blood-brain barrier (BBB) integrity is compromised in many CNS disorders. Complex astrocyte and vascular endothelial cell interactions that regulate BBB integrity may be disturbed in these disorders. We have previously shown that systemic administration of 3-chloropropanediol induces a transitory glial fibrillary acidic protein (GFAP)-astrocyte loss, reversible loss of tight junction complexes, and BBB integrity disruption. However, the intracellular signaling mechanisms that induce BBB integrity marker loss are unclear. We hypothesize that 3-chloropropanediol-induced modulation of tight junction protein expression is mediated through the phosphoinositide-3-kinase (PI3K)/AKT pathway. To test this hypothesis we have used a mouse brain endothelial cell line (bEnd.3) exposed to 3-chloropropanediol for up to 3 days. Results showed early reversible loss of sharp paracellular claudin-5 expression 90, 105, and 120 min following 3-chloropropanediol (500 μM) treatment. Sharp paracellular claudin-5 ...
Shop a large selection of Proteins A-Z products and learn more about Novus Biologicals Claudin-2 Recombinant Protein Antigen Quantity: 100µL:Life Quantity: 100µL.
The blood-brain barrier maintains central nervous system homeostasis and limits the entry of blood-borne substances that could alter neuronal function and survival. The barrier exists predominantly at the endothelium of cerebral vascular microvessels. The cerebral vascular endothelium becomes highly specialized during the formation of the neurovascular unit early in embryonic development. The blood-brain barrier is present and functional early in fetal life. The tightness of the barrier gradually increases throughout gestation and in the newborn period. Alterations in the basolateral environment of the cerebral microvasculature can modify the blood-brain barrier properties by modulating the expression of the endothelial tight junctions and other biochemical properties of the cerebral vascular endothelium. Maturation of the blood-brain barrier late in gestation correlates with increases in endogenous corticosteroids and with exposure to exogenous corticosteroids. Several adverse fetal and ...
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Claudin-4 (Clostridium perfringens enterotoxin receptor) is a tight junction protein encoded by the gene CLDN4. Expression of Claudin-4 has been associated with either poor prognosis or a more favorable diagnosis, depending on the type of cancer. Claudin-4 has been shown to distinguish adenocarcinoma from malignant mesothelioma with 99% specificity in malignant effusions (1). Claudin-4 overexpression was able to independently predict survival in a breast cancer multivariate analysis as it was associated with poor prognosis, high tumor grade and Her2 expression and was inversely correlated with estrogen receptor staining (2). In luminal breast cancer, the increase of Claudin-4 protein was correlated with the increase of tumor grade and with Ki-67, and thus demonstrated an overall shorter life survival (3). Basal-like tumors also demonstrated overexpression of Claudin-4 (4). Counter to the above breast cancer subtypes, the presence of Claudin-4 in triple negative breast cancer was a biomarker that
Luca Bors1, Ágnes Bajza1, Barbara Hutka1, László Dénes1, Krisztián Szigeti2, Nikolett Hegedűs2, Dávid Szöllősi2, Domokos Máthé2, Attila Csorba3 and Franciska Erdő1. Investigation of the impact of aging on blood-brain barrier function in rats - Does P-glycoprotein (P-gp) have any role in changing BBB permeability?. ...
Claudin-1 is an integral membrane protein component of tight junctions. The Snail family of transcription factors are repressors that play a central role in the epithelial-mesenchymal transition, a process that occurs during cancer progression. Snail and Slug members are direct repressors of E-cadherin and act by binding to the specific E-boxes of its proximal promoter. In the present study, we demonstrate that overexpression of Slug or Snail causes a decrease in transepithelial electrical resistance. Overexpression of Slug and Snail in MDCK (Madin-Darby canine kidney) cells down-regulated Claudin-1 at protein and mRNA levels. In addition, Snail and Slug are able to effectively repress human Claudin-1-driven reporter gene constructs containing the wild-type promoter sequence, but not those with mutations in two proximal E-box elements. We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin-1 promoter. Moreover, an inverse correlation in ...
Read Claudin-8 Expression in Renal Epithelial Cells Augments the Paracellular Barrier by Replacing Endogenous Claudin-2, The Journal of Membrane Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
The ability to invade host tissues and metastasize is the major cause of cancer-related death. During tumor invasion, metastasizing cells disrupt normal cell-matrix adhesion and acquire an invasive phenotype. Claudins are adhesion proteins localized at tight junctions (TJs). Claudin-7 is a unique TJ membrane protein in that it has a stronger basolateral membrane distribution than that of apical TJs in epithelial cells. To study the basolateral function of claudin-7, claudin-7 gene silencing experiments were carried out in a lung cancer cell line using the lentivirus shRNA approach. We found that claudin-7 knockdown (KD) cells showed disrupted cell-matrix interactions. Consequently, when claudin-7 KD cells were plated on the uncoated glass surface, they were unable to attach to the glass and died the day after plating. In contrast, control cells adhered well and grew normally. Using immunofluorescent microscopy and biochemistry methods, we found that claudin-7 co-localized and ...
Barclay Morrison III, associate professor of biomedical engineering at Columbia Engineering, has led the first study to determine underlying biological mechanisms that promote functional recovery of the blood-brain barrier (BBB) after blast injury.
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Associate Professor. One in four people worldwide - over 1.5 billion people - suffer from brain disorders, including depression, infection, trauma, stroke, seizures, dementia, and tumors. Despite this huge demand for treatments, delivery of drugs into the brain to treat these disorders is greatly impaired by the blood-brain barrier. The blood-brain barrier is the interface between blood and brain that controls what goes in and comes out of the brain. Anatomically, the blood-brain barrier is made of endothelial cells forming a complex vascular network that supplies the brain with oxygen and nutrients, and disposes of carbon dioxide and wastes. Recent studies show that the blood-brain barrier is affected by brain disorders and itself plays a role in causing brain disease. Therefore, understanding blood-brain barrier function is critical for devising new therapeutic strategies to enhance brain drug delivery, improve brain protection, and treat brain disorders. Currently, we study the role of the ...
For every experimental group, brains from at minimum 3 distinct litter had been analyzed and when compared to the in accordance NaCl handle group. qPCR approach improvement exposed that only samples must be when compared to every other which have gone through experimental treatment, mind isolation, storage, purification and evaluation preparing steps with each other. Therefore, for every DEX-treatment the according NaCl handle group was carried out at the exact same time. In addition, owing to the large complete variety of samples, but limited sample variety which could be purified at the same time, only samples from mice at the same age and identical variety of antenatal injections ended up compared to every other by using a two-tailed Student`s t-take a look at. Data are offered as the signifies ± SEM. The major tight junction molecule and mind endothelial mobile marker claudin-five was investigated originally. Triple maternal DEX remedy drastically decreased claudin-5 mRNA expression to .54 ...
Implications of the blood-brain barrier and its manipulation , Implications of the blood-brain barrier and its manipulation , کتابخانه دیجیتال دانشگاه علوم پزشکی اصفهان
The expression of claudin-11 in benign and malignant bladder tissue and the effect of forced expression of claudin-11 on tight junction function and invasiveness of bladder cancer cells were studied. Claudin-11 expression was tested in bladder cancer cell lines (T24/83, RT 112/84 and EJ138) using reverse transcription-polymerase chain reaction (RT-PCR) and in benign and malignant bladder tissue by quantitative RT-PCR and immunohistochemistry. T24/83 cells were transfected with the pcDNA.1/NT-GFP-TOPO vector containing full-length human claudin-11 sequence. Stable-transfected cells overexpressing claudin-11 (T24Cl-11Ex), wild-type cells (T24WT) and the empty plasmid control clone (T24GFP) were compared using transurothelial resistance (TUR), in vitro adhesion, invasion and growth assays. Claudin-11 was strongly expressed in the non-invasive RT112/84 cell line compared to the invasive T24/83 and EJ138 TCC cell lines. Benign bladder tissue demonstrated equal expression of claudin-11 mRNA as ...
Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. Chromatin immunoprecipitation (ChIP) and luciferase reporter assay showed that SCF/c-kit
DESCRIPTION (provided by applicant): Disruption of the cell-cell junction with concomitant changes in the expression of junctional proteins is a hallmark of cancer metastasis and invasion. Role of adherent junction proteins have been studied extensively in cancer, however the role of tight junction proteins is less understood. Claudins are the recently identified tetraspanins, which are integral to the structure and function of tight junctions (TJs). Recent studies have shown changes in expression/cellular localization for claudins during tumorigenesis, however a cause and effect relationship has not been established. Here, we report a highly increased expression for claudin-1 in human primary colon carcinoma and metastatic tissues and cell lines derived from similar sources with relatively frequent nuclear localization. Furthermore, using genetic manipulations of claudin-1 expression in colon cancer cell lines, we demonstrate a role for claudin-1 in the regulation of epithelial to mesenchymal ...
Recent investigations revealed that epithelial TJs contain charge- and size-selective pores, which control the paracellular flux of charged and uncharged solutes. The paracellular flux occurs through two distinct pathways: one high-capacity pathway with size-restrictive pores and one low-capacity pathway that is size independent, at least for substances with radii of up to 7 Å (Van Itallie et al., 2008; Watson et al., 2001). The physical basis of the low-capacity pathway is not yet completely understood, whereas the high-capacity pathway is well described. It consists of small pores with radii of ~4 Å, and is responsible for the flux of small charged and uncharged solutes. Expression of claudin-2 affects only the high-capacity pathway, by inducing an increase in pore number and a change in charge selectivity (Van Itallie et al., 2008). In a recent attempt to model the claudin-2-induced channel (Yu et al., 2009), the pore diameter, calculated from permeability to organic cations and from the ...
The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.
Mouse monoclonal ZO1 tight junction protein antibody [mAbcam 61357] validated for WB, IP, Flow Cyt and tested in Human. Referenced in 2 publications and 5…
In the present study, we demonstrate that HIV-infected leukocytes transmigrate in greater numbers across a tissue culture model of the human BBB in response to CCL2 than do uninfected cells, resulting in increased BBB permeability. This process was characterized by four major findings. First, BBB permeability was increased only when the combination of HIV-infected cells and a CCL2 chemotactic gradient was present. The addition of CCL2 alone or the adhesion of HIV-infected cells alone to the BBB model was not sufficient to induce BBB disruption. Second, the mechanism of BBB disruption and enhanced transmigration of HIV-infected cells was specifically CCL2 dependent, because other chemokines did not replicate the CCL2 effect. Third, the high HIV-infected leukocyte transmigration induced by CCL2 was associated with increased BBB permeability that correlated with a reduction in TJP (ZO-1, claudin-1, and occludin) and an increase in MMP-2 and MMP-9 in BBB cells. Fourth, HIV-infected leukocytes ...
http://www.jcb.org/cgi/doi/10.1083/jcb1693fta1 Endothelial tight junctions form the blood–brain barrier] What is the cellular correlate of the so called blood-brain barrier? Thomas Reese and Morris Karnovsky find that it is the junctions between endothelial cells in the brain vasculature. Their discovery comes thanks to three factors: high resolution electron microscopy; the development of sensitive tracer methods; and a fortuitous lunch date ...
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View mouse Cldn15 Chr5:136966616-136975858 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
We produce a variety of bags used for cell culture applications. These specialized container products are constructed using our NxFlex® F1000C1 films. The inner solution contact layer of this film is specially formulated to be non-leaching and chemically inert for the highest biocompatibility requirements of cell culture applications. A layer of EVOH insures excellent gas barrier properties.. ...
Blood-brain barrier (BBB) leakage plays a key role in cerebral ischemia-reperfusion injury. It is quite necessary to further explore the characteristic and mechanism of BBB leakage during stroke. We induced a focal cerebral ischemia model by transient middle cerebral artery occlusion in male rats for defining the time course of BBB permeability within 120 h following reperfusion and evaluate the specific role of tight junction (TJ) associated proteins claudin-5, occludin, and ZO-1 as well as protein kinase C delta (PKCδ) pathway in BBB leakage induced by reperfusion injury. We verified a bimodal increase in the permeability of the BBB following focal ischemia by Evans blue assay. Two peaks of BBB permeability appeared at 3 h and 72 h of reperfusion after 2 h focal ischemia, respectively. The leak at the endothelial cell was represented at the level of transmission electron microscopy. TTC staining results showed increased infarct size with time after cerebral ischemia reperfusion. The mRNA and ...
The tight junction protein claudin-1 (CLDN1) has been shown to be essential for hepatitis C virus (HCV) entry-the first step of viral infection. Due to the lack of neutralizing anti-CLDN1 antibodies, the role of CLDN1 in the viral entry process is poorly understood. In this study, we produced antibodies directed against the human CLDN1 extracellular loops by genetic immunization and used these antibodies to investigate the mechanistic role of CLDN1 for HCV entry in an infectious HCV cell culture system and human hepatocytes. Antibodies specific for cell surface-expressed CLDN1 specifically inhibit HCV infection in a dose-dependent manner. Antibodies specific for CLDN1, scavenger receptor B1, and CD81 show an additive neutralizing capacity compared with either agent used alone. Kinetic studies with anti-CLDN1 and anti-CD81 antibodies demonstrate that HCV interactions with both entry factors occur at a similar time in the internalization process. Anti-CLDN1 antibodies inhibit the binding of ...
HCV is a leading cause of hepatocellular carcinoma and cirrhosis all over the world. Claudins belong to family of tight junctions proteins that are responsible for establishing barriers for controlling the flow of molecules around cells. For therapeutic strategies, regulation of viral entry into the host cells holds a lot of promise. During HCV infection claudin-1 is highly expressed in liver and believed to be associated with HCV virus entry after HCV binding with or without co-receptor CD81. The claudin-1 assembly with tight junctions is regulated by post translational modifications. During claudins assembly and disassembly with tight junctions, phosphorylation is required at C-terminal tail. In cellular proteins, interplay between phosphorylation and O-β-GlcNAc modification is believed to be functional switch, but it is very difficult to monitor these functional and vibrant changes in vivo. Netphos 2.0 and Disphos 1.3 programs were used for potential phosphorylation; NetPhosK 1.0 and KinasePhos for
Objective Helicobacter pylori strains that express the oncoprotein CagA augment risk for gastric cancer. However, the precise mechanisms through which cag+ strains heighten cancer risk have not been fully delineated and model systems that recapitulate the gastric niche are critical for understanding pathogenesis. Gastroids are three-dimensional organ-like structures that provide unique opportunities to study host-H. pylori interactions in a preclinical model. We used gastroids to inform and direct in vitro studies to define mechanisms through which H. pylori modulates expression of the cancer-associated tight junction protein claudin-7.. ...
Claudins are major integral membrane proteins of tight junctions. Altered expression of several claudin proteins, in particular claudin-1, -3, -4 and -7, has been linked to the development of various cancers. Although their dysregulation in cancer suggests that claudins play a role in tumorigenesis, the exact underlying mechanism remains unclear. The involvement of claudins in tumor progression was suggested by their important role in the migration, invasion and metastasis of cancer cells in a tissue-dependent manner. Recent studies have shown that they play a role in epithelial to mesenchymal transition (EMT), the formation of cancer stem cells or tumor-initiating cells (CSCs/TICs), and chemoresistance, suggesting that claudins are promising targets for the treatment of chemoresistant and recurrent tumors. A recently identified claudin-low breast cancer subtype that is characterized by the enrichment of EMT and stem cell-like features is significantly associated with disease recurrence, underscoring
Our research should stimulate renewed clinical interest in developing glucocorticoid therapies to treat blast-induced traumatic brain injury (bTBI) and other disorders of the central nervous system, Morrison says. His findings also hold important implications for military personnel exposed to blast injury. We may be able to improve outcomes in brain-injured soldiers and civilians, he continues, and reduce the length of their mandatory rest periods before returning to duty, making the difference between requiring only days rather than weeks or longer to recover.. This improvement could be a significant result, as there are currently no approved pharmaceutical therapies for traumatic brain injury (TBI), and recently completed clinical trials have not demonstrated any benefit of other tested neuro-protective interventions. For patients with head injuries (non-blast related) and brain edema, doctors have been prescribing glucocorticoids, a class of steroid hormones, as standard treatment for ...
Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier...
Blood vessels in the central nervous system (CNS) are unique in forming the blood-brain barrier (BBB), which confers high electrical resistance and low permeability properties, thus protecting neural cells from potentially harmful blood components. Endothelial cells, which form the inner cellular lining of all blood vessels, play a critical role in this process by forming tight adhesive interactions between each other. To study the properties of primary brain endothelial cells (BECs), a number of different methods have been described. In this chapter, we present a relatively simple method that produces high numbers of primary mouse BECs that are highly pure (greater than 99 % CD31-positive). In addition, we also describe an immunocytochemical approach to demonstrate the endothelial purity of these cultures ...
In the small intestine, gluten triggers the release of zonulin, a protein that regulates the tight junctions between epithelial cells and therefore intestinal, but also blood-brain barrier function. Recent evidence suggests that overstimulation of zonulin in susceptible individuals could dysregulate intercellular communication promoting tumorigenesis at specific organ sites ...
They found that the severity of the disease was reduced by both warfarin (see figure below) and rivoraxaban. In contrast, despite effective anticoagulation, starting after the initiation of the disease had no effect on the course of the EAE (i.e. no effect when used as a therapeutic regimen rather than a preventative one). One explanation is that the effect of anticoagulation is small, which is only apparent in the preventative setting but is diluted in the therapeutic setting. Another explanation is that the mechanisms of the disease after onset are very much different to those at the start, which is also likely to be true. A key feature of the MS disease onset is the loss of blood brain barrier (BBB) integrity, which allows the influx of immune cells into the brain. Thrombin is known to interrupt BBB integrity, and the protective effect of anticoagulation may be explained by the low thrombin activity preserving the BBB integrity. ...
They found that the severity of the disease was reduced by both warfarin (see figure below) and rivoraxaban. In contrast, despite effective anticoagulation, starting after the initiation of the disease had no effect on the course of the EAE (i.e. no effect when used as a therapeutic regimen rather than a preventative one). One explanation is that the effect of anticoagulation is small, which is only apparent in the preventative setting but is diluted in the therapeutic setting. Another explanation is that the mechanisms of the disease after onset are very much different to those at the start, which is also likely to be true. A key feature of the MS disease onset is the loss of blood brain barrier (BBB) integrity, which allows the influx of immune cells into the brain. Thrombin is known to interrupt BBB integrity, and the protective effect of anticoagulation may be explained by the low thrombin activity preserving the BBB integrity. ...
Cytoplasmic expression of claudin-1 in metastatic melanoma cells correlates to increased migration, and increased secretion of MMP-2 in a PKC dependent manner, whereas claudin-1 nuclear expressi...
Sigma-Aldrich offers abstracts and full-text articles by [S K Tiwari-Woodruff, A G Buznikov, T Q Vu, P E Micevych, K Chen, H I Kornblum, J M Bronstein].
Many other factors can cause a leaky blood-brain barrier. Fortunately, more natural ways have been demonstrated to help fix a leaky blood-brain barrier.
Stephen Strasburg has been scratched from tonights start against the Phillies with what the Nationals are calling forearm tightness. Ross Ohlendorf is starting his his place.
Stephen Strasburg has been scratched from tonights start against the Phillies with what the Nationals are calling forearm tightness. Ross Ohlendorf is starting his his place.
Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans cells in the epidermis. Our objective was to identify immunoreactive CLDN1 in human epidermal Langerhans cells and to examine the pattern of epidermal Langerhans cells in genetic human CLDN1 deficiency [neonatal ichthyosis, sclerosing cholangitis (NISCH) syndrome]. Epidermal cells from healthy human skin labelled with CLDN1-specific antibodies were analysed by confocal laser immunofluorescence microscopy and flow cytometry. Skin biopsy sections of two patients with NISCH syndrome were stained with an antibody to CD1a expressed on epidermal Langerhans cells. Epidermal Langerhans cells and a subpopulation of keratinocytes from healthy skin were positive for CLDN1. The gross number and distribution of epidermal Langerhans cells of two patients with molecularly confirmed NISCH syndrome, however, was not grossly altered. Therefore, CLDN1 is unlikely to play a critical role in ...
Familial hypomagnesemia with hypercalciuria and nephrocalcinosis is an autosomal recessive tubular disorder characterized by excessive renal magnesium and calcium excretion and chronic kidney failure. This rare disease is caused by mutations in the CLDN16 and CLDN19 genes. These genes encode the tight junction proteins claudin-16 and claudin-19, respectively, which regulate the paracellular ion reabsortion in the kidney. Patients with mutations in the CLDN19 gene also present severe visual impairment. Our goals in this study were to examine the clinical characteristics of a large cohort of Spanish patients with this disorder and to identify the disease causing mutations. We included a total of 31 patients belonging to 27 unrelated families and studied renal and ocular manifestations. We then analyzed by direct DNA sequencing the coding regions of CLDN16 and CLDN19 genes in these patients. Bioinformatic tools were used to predict the consequences of mutations. Clinical evaluation showed ocular defects in
Diabetes mellitus (DM) is a high risk factor for stroke and leads to more severe vascular and white-matter injury than stroke in non-DM. We tested the neurorestorative effects of delayed human umbilical cord blood cell (HUCBC) treatment of stroke in type-2 diabetes (T2DM). db/db-T2DM and db/+-non-DM mice were subjected to distal middle cerebral artery occlusion (dMCAo) and were treated 3 days after dMCAo with: (a) non-DM + Phosphate buffered saline (PBS); (b) T2DM + PBS; (c) T2DM + naïve-HUCBC; (d) T2DM + miR-126(-/-) HUCBC. Functional evaluation, vascular and white-matter changes, neuroinflammation, and miR-126 effects were measured in vivo and in vitro. T2DM mice exhibited significantly decreased serum and brain tissue miR-126 expression compared with non-DM mice. T2DM + HUCBC mice exhibited increased miR-126 expression, increased tight junction protein expression, axon/myelin, vascular density, and M2-macrophage polarization. However, decreased blood-brain barrier leakage, brain hemorrhage, and miR
Epithelial barrier dysfunction is a significant factor in many allergic diseases, including eosinophilic esophagitis (EoE). Infiltrating leukocytes and tissue adaptations increase metabolic demands and decrease oxygen availability at barrier surfaces. Understanding of how these processes impact barrier is limited, particularly in allergy. Here, we identified a regulatory axis whereby the oxygen-sensing transcription factor HIF-1α orchestrated epithelial barrier integrity, selectively controlling tight junction CLDN1 (claudin-1). Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial barrier function, as documented in 3D organotypic epithelial cultures. L2-IL5OXA mice with EoE-relevant allergic inflammation displayed localized eosinophil oxygen metabolism, tissue hypoxia, and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of esophageal epithelial-targeted stabilized ...
The present invention relates to a nanocomposite blend composition having superior barrier properties, and more particularly to a nanocomposite blend composition comprising a polyolefin resin; one or more nanocomposites having barrier properties, selected from an ethylene-vinyl alcohol (EVOH)/intercalated clay nanocomposite, a polyamide/intercalated clay nanocomposite, an ionomer/intercalated clay nanocomposite, and a polyvinyl alcohol (PVA)/intercalated clay nanocomposite; and a compatibilizer. This nanocomposite blend composition has superior mechanical strength and superior barrier properties to oxygen, organic solvent, and moisture. Also, it has superior chemical barrier properties and is applicable to single/multi-layer blow molding and film processing.
Stabilization of the blood-brain barrier during and after stroke can lead to less adverse outcome. For elucidation of underlying mechanisms and development of novel therapeutic strategies validated in vitro disease models of the blood-brain barrier could be very helpful. To mimic in vitro stroke conditions we have established a blood-brain barrier in vitro model based on mouse cell line cerebEND and applied oxygen/glucose deprivation (OGD). The role of astrocytes in this disease model was investigated by using cell line C6. Transwell studies pointed out that addition of astrocytes during OGD increased the barrier damage significantly in comparison to the endothelial monoculture shown by changes of transendothelial electrical resistance as well as fluorescein permeability data. Analysis on mRNA and protein levels by qPCR, western blotting and immunofluorescence microscopy of tight junction molecules claudin-3,-5,-12, occludin and ZO-1 revealed that their regulation and localisation is associated with the
Muscular tightness is one of the disruptions to normal movement and if not managed well can lead to possible injury. Identifying your tightness and using specific strategies will help relieve tension.
The brain is kind of like an exclusive nightclub; if youre not on the list, the bouncer, AKA the blood-brain barrier, can and will turn you away...
Nevertheless, another sources document existence of mTEC unipotent progenitors that express claudin 3 and 4 (Cld3/4). These two ... "Medullary thymic epithelial cells expressing Aire represent a unique lineage derived from cells expressing claudin". Nature ... 3 (5): 383-91. doi:10.1038/nri1085. PMID 12766760. Kurobe H, Liu C, Ueno T, Saito F, Ohigashi I, Seach N, Arakaki R, Hayashi Y ... 41 (5): 753-61. doi:10.1016/j.immuni.2014.10.011. PMID 25464854. Ohigashi I, Zuklys S, Sakata M, Mayer CE, Hamazaki Y, Minato N ...
Studies suggest that the tight junction protein Claudin-1 (CLDN1) may also play a part in HCV entry. Claudin family ... "Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry". Nature. 446 (7137): 801-5. doi:10.1038/ ... LRP5 (low-density lipoprotein receptor-related protein 5) acts as a co-receptor for the Wnt-family of glycoproteins which ... Interleukins 1, 2, and 5 all rely on interleukin co-receptors to bind to the primary interleukin receptors. Syndecans 1 and 4 ...
Claudin de Sermisy, French composer (b. 1495) October 18 - Anne d'Alençon, French noblewoman (b. 1492) November 7 - Maldeo ... December 5 - King Francis II of France dies aged 16 of an ear infection at Orléans, widowing the 17-year-old Mary, Queen of ... November 5 - Battle of Corrichie in Scotland: The rebellion of George Gordon, Earl of Huntly is crushed by James Stewart, Earl ... Retrieved 5 February 2021. "All People That on Earth Do Dwell". The Cyber Hymnal. Archived from the original on July 3, 2011. ...
Claudin 5 is transiently expressed during the development of the retinal pigment epithelium. J Membr Biol 186: 81-88, 2002 ... 5] 기능[편집]. 망막색소상피세포는 신체 내에서 몇몇 기능을 수행한다. 광수용(light absorption), 물질 수송(epithelial transport), 시각회로 조절(visual cycle), 식세포작용( ... doi:10.1016/S0039-6257(00)00140-5.. *↑ Rizzolo, lj (2014). "Barrier properties of cultured retinal pigment epithelium". 》 ... Coexpression of FGF-5 and bFGF by the retinal pigment epithelium in vitro. Exp Eye Res 55: 727-734, 1992 ...
CLDND1: Claudin domain containing 1. *CPN2: Carboxypeptidase N subunit 2. *CPOX: coproporphyrinogen oxidase (coproporphyria, ... doi:10.1186/gb-2010-11-5-206. PMC 2898077. PMID 20441615.. *^ "Statistics & Downloads for chromosome 3". HUGO Gene Nomenclature ... So CCDS's gene number prediction represents a lower bound on the total number of human protein-coding genes.[5] ...
Sertoliho bunky tvoria pomocou tesných spojov (proteínmi ako je claudin alebo occludin) hemato-semenníkovú bariéru, ktorá ... 5, s. 1423-1428. Dostupné online [cit. 2018-08-22]. ISSN 0041-1345. DOI: 10.1016/j.transproceed.2012.01.143. ... Sertoliho bunky kontrolujú vstup aj výstup molekúl cez túto bariéru - väčšina musí prejsť priamo cez cytoplazmu SC[5]. ...
Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane ... vermutlich Claudin bei CPE,[14] sowie vermutlich GPI-Anker oder andere Glykosylierungen beim ε-Toxin. Durch die ... N. Ramarao, V. Sanchis: The pore-forming haemolysins of bacillus cereus: a review. In: Toxins. Band 5, Nummer 6, Juni 2013, S. ... 6460, January 1994, S. 292-5. doi:10.1038/367292a0. PMID 7510043. *↑ Cole AR, Gibert M, Popoff M, Moss DS, Titball RW, Basak AK ...
Jia, P.; B. Andreotti; P. Claudin (March 2017). "Giant ripples on comet 67P/Churyumov-Gerasimenko sculpted by sunset thermal ... 5: 247. A large portion of this article was sourced from the Gazetteer of Planetary Nomenclature, the official IAU database of ...
The chorale theme (Zahn 7568) by Claudin de Sermisy first appeared in print in the collection of secular songs Trente et quatre ... Retrieved 5 January 2020. Books Dürr, Alfred; Jones, Richard D. P. (2006). The Cantatas of J. S. Bach: With Their Librettos in ... Retrieved 5 January 2020. Steinitz, Margaret. Bach's Latin Church Music. London Bach Society. Archived from the original on 15 ...
In a healthy human intestine, small particles (< 4 Å in radius) can migrate through tight junction claudin pore pathways, and ... p. 7. ISBN 978-0-7923-8772-5. Thoma YM, Anderson JM, Turner JR (2012). Johnson LR, et al. (eds.). Tight Junctions and the ...
Claudin 1, which is a tight-junction protein, and CD81 link to create a complex, priming them for later HCV infection processes ... 5 (6): 453-63. doi:10.1038/nrmicro1645. PMID 17487147. S2CID 13176201.. *^ Rigat K, Wang Y, Hudyma TW, et al. (November 2010 ... 7 (5): 710-721. doi:10.1017/S1355838201010111. PMC 1370123. PMID 11350035.. *^ Baghbani-arani F, Roohvand F, Aghasadeghi MR, ... 5 (3): 129-51. doi:10.1089/mcg.2000.5.129. PMID 11252351.. *^ Jubin R (2001). "Hepatitis C IRES: translating translation into a ...
... where he includes the musician between Clément Janequin and Claudin de Sermisy as part of a choir singing a ribald song, in ... ISBN 0-674-61525-5 Gustave Reese, Music in the Renaissance. New York, W.W. Norton & Co., 1954/1959. ISBN 0-393-09530-4 ...
... as well as production of epithelial proteins such as desmoplakin and claudin, and activate the expression of vimentin and ... and 5) "pulling" the trailing edge toward movement through the newly formed defects in the matrix structure. Since the cells ...
383 (5): 725-37. doi:10.1515/BC.2002.076. PMID 12108537.. *^ a b Lampe, Paul D.; Lau, Alan F. (2004). "The effects of connexin ... 116 (5): 1243-53. doi:10.1172/JCI27186. PMC 1440704 . PMID 16628254.. *^ Zhang, Y.; Wang, H.; Kovacs, A.; Kanter, E. M.; Yamada ... 69 (5): 1472-80. doi:10.1095/biolreprod.103.016360. PMID 12826585.. *^ El-Sabban, M. E.; Sfeir, AJ; Daher, MH; Kalaany, NY; ... doi:10.1007/s12031-007-9027-5. PMC 2650399 . PMID 18236012.. *^ Revel, J. P.; Karnovsky, M. J.; Aitchison, EJ; Smith, EG; ...
Anatole Claudin (1898). The First Paris Press: an account of the books printed for G. Fichet and J. Heynlin in the Sorbonne, ... 5] In 1476 in Lyon appeared one of the first printed French-language books, La Légende Dorée (Golden Legend) by Jacobus da ...
Famous composers of these "Parisian" chansons included Claudin de Sermisy and Clément Janequin. Janequin's La guerre, written ... 5] Leonardo, a famous painter and inventor, arrived with three of his paintings, namely the Mona Lisa, Sainte Anne, and Saint ...
Claudin de Sermisy, Thomas Crecquillon, Domenico Ferrabosco, Jean de Latre, Jacquet de Berchem, Jakob Meiland, Alexander ... 300 R/Vv, 123". The codex, consisting of 183 folia, is bound in parchment with the initials "P W S P" and the number "1 5 9 1" ... Contains 5 pieces Martin Rost, Die Norddeutsche Orgelkunst Vol.2 - Danzig, published by MDG, MDG MDG 319 0178-2, 2003; Contains ... 5 pieces Wolfgang Baumgratz, Orgels in Hanzesteden, published by NCRV, NCRV 9088, 1992; Contains 4 pieces Bogusław Grabowski, ...
The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and therefore potentially oncogenic missense mutations (click on Show Cancer Mutations). The bar plot below shows the proportion of tumor samples that have any kind of altering mutation(s) in the given protein. ...
Invitrogen Anti-Claudin 5 Monoclonal (4C3C2), Catalog # 35-2500. Tested in Western Blot (WB), Immunofluorescence (IF), ... Claudin 5 Monoclonal Antibody (4C3C2). Advanced Verification This Antibody was verified by Relative expression to ensure that ... Claudin-5 was detected at ~23 kDa using Claudin-5 Mouse Monoclonal Antibody (Product # 35-2500) at 0.5-1 µg/mL in 2.5 % skim ... Cite Claudin 5 Monoclonal Antibody (4C3C2). The following product was used in this experiment: Claudin 5 Monoclonal Antibody ( ...
Invitrogen Anti-Claudin 5 Monoclonal (4C3C2), Catalog # 352588. Tested in Western Blot (WB), Immunofluorescence (IF), ... claudin-1 and claudin-2, with no similarity to occludin. In contrast to occludin, which induces only a small number of short ... Published figure using Claudin 5 monoclonal antibody (Product # 352588). Fig 5 Excess tip cell production in the Sox7, Sox17, ... Published figure using Claudin 5 monoclonal antibody (Product # 352588). Immunofluorescent staining of Claudin-5 in human Caco- ...
Over-expression of claudin-5 was sufficient to prevent replication-deficient virus-induced permeability. The barrier-protective ... Instead, replication-deficient virus induced degradation of the tight junction protein claudin-5; the adherens junction protein ... agent formoterol was able to markedly attenuate flu-induced leak in association with dose-dependent induction of claudin-5. ... Influenza Infects Lung Microvascular Endothelium Leading to Microvascular Leak: Role of Apoptosis and Claudin-5. Figure 10. ...
Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF Resistant Diabetic Macular Edema ... Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF Resistant Diabetic Macular Edema ... Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF Resistant Diabetic Macular Edema ... Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF Resistant Diabetic Macular Edema ...
4A). Claudin-5 is anchored to the actin cytoskeleton via ZO-1, and this structure is crucial for stable claudin-5 expression in ... n = 3. C: Effect of Rip-OS on claudin-5 transcription levels in the retina of Kimba mice. n = 4. D: Effects of ripasudil and ... For example, claudin-5 is an essential TJ component protein (14-16) and its assembly is dependent on AJ-associated vascular ... 7B, C, F, and G). However, ZO-1 and claudin-5 binding was attenuated by VEGF stimulation, and this effect was inhibited by ...
Interendothelial claudin-5 expression depends on cerebral endothelial cell-matrix adhesion by ß1-integrins. ... Osada, T., Gu, Y-H., Kanazawa, M., Tsubota, Y., Hawkins, B., Spatz, M., ... del Zoppo, G. (2011). Interendothelial claudin-5 ...
Compare and order Claudin 5 ELISA Kits. View citations, images, detection ranges, sensitivity, prices and more. Recommended ... claudin 5b (cldn5b) ELISA Kit * claudin 5 (transmembrane protein deleted in velocardiofacial syndrome) L homeolog (cldn5.L) ... Search Claudin 5 ELISA Kits for other reactivities: Monkey,. Pig (Porcine),. Cow (Bovine),. Guinea Pig,. Rabbit,. Goat,. ... Claudin 5 ELISA Kit. 47 products by 11 suppliers: Blue Gene , Abbexa , USBio , Wuhan Fine biotech , Biomatik , CUSABIO , Cloud- ...
5. Heat samples at 95-100C for 5 minutes.. 6. Load 5ul of a control pre-stained protein ladder to track sample migration in the ... The blocking solution is normally composed of 5% non-fat milk in TBS-T, and some antibodies require BSA in place of milk. This ... Remember to make PVDF soaked in methanol for 5 minutes prior to use.. 4. Electrophorese for 90 minutes at 100V or overnight at ... 5. Check membrane for transfer of control protein ladder. If using nitrocellulose, sample transfer and relative concentration ...
The graph plots aggregation by the particle number at a time point divided by the particle number at time 0 (Nt/No). Claudin-1 ... PKH26-labeled untransfected L-cells (red) were mixed 1:1 with unlabeled untransfected L-cells (top row), GFP-Claudin-1- ... Expression of Claudin-1 or Cacng2 promotes cell aggregation, as shown by microscopy and particle counts. ... Because stargazin is structurally similar to claudins, we hypothesized that it might also have retained claudin-like functions ...
Stable expression of claudin-5(wild type) in MDCK-II cells-but not that of mutants R145A, Y148A, Y158A or E159Q-increas … ... Here, we analyzed the impact of residues in the second extracellular loop (ECL2) of mouse claudin-5 on paracellular ... Stable expression of claudin-5(wild type) in MDCK-II cells-but not that of mutants R145A, Y148A, Y158A or E159Q-increased ... Expression of claudin-5(Y148A), (Y158A) or (E159Q) enhanced permeability of FITC-dextran(10 kDa), which was unchanged in cells ...
A) Occludin, ZO-1 and claudin-5 expression in sham ... occludin and claudin-5 rearrangement and lessened IgG and Evans ... A) Occludin, ZO-1 and claudin-5 expression in sham group, NS and metformin-treated mice at 3 days following tMCAO. Scale bar = ... A) Occludin, ZO-1 and claudin-5 expression in sham group, NS and metformin-treated mice at 3 days following tMCAO. Scale bar = ... Bar graphs show a quantification of occludin, ZO-1 and claudin-5 expression. (C) IgG leakage at 3 days following tMCAO in ...
Sharp paracellular claudin-5 profiles were later restored, but lost again by 2 and 3 days post 3-chloropropanediol treatment. ... Biphasic modulation of paracellular claudin-5 expression in mouse brain endothelial cells is mediated through the PI3K/AKT ... Biphasic modulation of paracellular claudin-5 expression in mouse brain endothelial cells is mediated through the PI3K/AKT ... Biphasic modulation of paracellular claudin-5 expression in mouse brain endothelial cells is mediated through the PI3K/AKT ...
1999) Endothelial claudin: claudin-5/TMVCF constitutes tight junction strands in endothelial cells. J Cell Biol 147:185-194. ... claudin. CNS. central nervous system. DMEM. Dulbeccos modified Eagles medium. ECL. extracellular loop domain. FBS. fetal ... 2015) Claudin-4 SPECT imaging allows detection of aplastic lesions in a mouse model of breast cancer. J Nucl Med 56:745-751. ... Claudin-5-Binders Enhance Permeation of Solutes across the Blood-Brain Barrier in a Mammalian Model. Yosuke Hashimoto, Keisuke ...
Changes in VE-cadherin and claudin-5 expression were studied by immunocytochemistry (ICC) and quantified by cell-based enzyme ... Tyrosine phosphorylation of VE-cadherin and claudin-5 is associated with TGF-β1-induced permeability of centrally derived ... Tyrosine phosphorylation of VE-cadherin and claudin-5 is associated with TGF-β1-induced permeability of centrally derived ... blood-retinal barrier; blood-brain barrier; transforming growth factor-β1; VE-cadherin; claudin-5; tyrosine phosphorylation. ...
Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in ... Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in ... Changes in expression and distribution of claudin 2, 5 and 8 lead to discontinuous tight junctions and barrier dysfunction in ...
A 2 kb sequence, from 6.5 to 8.5 kb upstream of claudin 5b, contains enhancer activities that can drive the expression in the ... Results: : The 5.6 kb fragment upstream of the start codon of claudin 5b can direct the EGFP expression in the hyaloid vessels ... DNA fragments of various lengths were amplified from the flanking regions of the claudin 5a or 5b, and inserted into the ... The purpose of this study is to identify DNA regulatory sequences of the zebrafish claudin 5 genes. ...
show that the epithelial-mesenchymal-transition transcription factor Snail induces claudin-11 expression and suppresses RhoA ... Shang, X., Lin, X., Alvarez, E., Manorek, G. & Howell, S. B. Tight junction proteins claudin-3 and claudin-4 control tumor ... Supplementary Figure 7: Expression of Snail or claudin-11 correlates with an advanced HNSCC and a worse prognosis.. ... 7: Expression of Snail or claudin-11 correlates with an advanced HNSCC and a worse prognosis.. ...
Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis. ... Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis. ... Here, we demonstrated that knockout of Cldn18, a claudin family member highly expressed in lung alveolar epithelium, leads to ... 2. Scale bars: 5 μm. (. H. ) IP with anti-p-LATS1/2 Ab shows decreased p-LATS1/2 and decreased association of p-LATS1/2 with p- ...
show that a disease-causing effect of claudin-19 mutations is due to a defective visual cycle from a decreased expression of ... This study explains why claudin-19 mutations alter the expression of RPE signature gene… ... In the kidney, claudin-19 must be complexed with claudin-16 to reach the cell surface27,45. However, human RPE delivers claudin ... ARPE-19 and the kidney cell lines, MDCK and LLC-PK1, do not express claudin-16 or claudin-19. Nonetheless, when claudin-19 is ...
suggest that claudin-6 induces MMP-2 activation through claudin-1 membrane expression Title: Claudin-6 enhances cell ... CLDN6 claudin 6 [Homo sapiens] CLDN6 claudin 6 [Homo sapiens]. Gene ID:9074 ... claudin 6provided by HGNC. Primary source. HGNC:HGNC:2048 See related. Ensembl:ENSG00000184697 MIM:615798 Gene type. protein ... Claudin 6 expression is useful to distinguish myxofibrosarcomas from other myxoid soft tissue tumors. Bekki H, et al. Pathol ...
After virotherapy with claudin-5, the cardiac contractile force deficits in Sgcd-/- mice are no longer significant. These ... After virotherapy with claudin-5, the cardiac contractile force deficits in Sgcd-/- mice are no longer significant. These ... Virotherapy with claudin-5 prevents the onset of cardiomyopathy in another muscular dystrophy model. ... Virotherapy with claudin-5 prevents the onset of cardiomyopathy in another muscular dystrophy model. ...
The claudin gene family: expression in normal and neoplastic tissues. BMC Cancer. 2006;6:186.PubMedCentralPubMedCrossRefGoogle ... Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals. Gene. 2011;481:83-92.PubMedCrossRef ... Claudin-18 is an early-stage marker of pancreatic carcinogenesis. J Histochem Cytochem. 2011;59:942-952.PubMedCentralPubMed ... miR-1303 Claudin-18 Stomach neoplasms Proliferation Invasion This is a preview of subscription content, log in to check access. ...
... and ADAM17-mediated claudin-5 degradation. For the in vivo study, C57BL/6 mice were administered a single dose of TiO2-NP ... Knockdown of barrier-forming claudins (e.g., claudin-1 or claudin-5) dramatically increases paracellular permeability. ... Figure S3. Changes in mRNA level of claudin-5, ZO-1 and occludin in TiO22-NP-treated bEnd.3 cells. Figure S4. TiO2-NP activated ... Claudin-5 has also been identified as a factor required for cell-cell contact and negatively regulates cell migration [23]. ...
Specific role of tight junction proteins claudin-5, occludin, and ZO-1 of the blood-brain barrier in a focal cerebral ischemic ... The interaction between Cav-1 and claudin-5 was further confirmed by coimmunoprecipitation. Consistent with these in vitro ... Moreover, occludin protein loss and claudin-5 redistribution were detected in ischemic cerebromicrovessels. These data indicate ... Matrix metalloproteinase-2-mediated occludin degradation and caveolin-1-mediated claudin-5 redistribution contribute to blood- ...
Bradykinin increases blood-tumor barrier permeability by down-regulating the expression levels of ZO-1, occludin, and claudin-5 ... Immunohistochemistry and immunofluorescence assays show that the attenuated expression of ZO-1, occludin, and claudin-5 and F- ... and claudin-5 and the rearrangement of F-actin and that cAMP/PKA signal transduction system might be involved in the modulating ... and claudin-5 and rearrangement of F-actin in brain microvascular endothelial cells are observed at the same time. Meanwhile, ...
In particular, claudin-3 and claudin-4 are frequently overexpressed in pancreatic cancer. 18F-Labeled claudin selective ... In this work we describe the synthesis of the first 18F-labeled probes potentially suitable for PET imaging of claudin-4 ... As such, cancer expression of claudin proteins is markedly dysregulated, making it an attractive target for molecular imaging ... to claudin selective peptides. As a proof-of-principle, one of them, 5-[18F]FDR-Clone 27, was isolated in ,98% radiochemical ...
claudin-2. Fwd. forward. GEO. Gene Expression Omnibus. GF. germ free. GPCR. G-protein coupled receptor. GPR109a. G-protein ... Claudin-2 as a mediator of leaky gut barrier during intestinal inflammation. Tissue Barriers 3: e977176. ... Claudin switching: physiological plasticity of the tight junction. Semin. Cell Dev. Biol. 42: 22-29. ... HIF-dependent regulation of claudin-1 is central to intestinal epithelial tight junction integrity. Mol. Biol. Cell 26: 2252- ...
While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the ... Often, several claudin family members are coexpressed and interact with each other, and this determines the overall ... IPR006187. Claudin. IPR003548. Claudin1. IPR017974. Claudin_CS. IPR004031. PMP22/EMP/MP20/Claudin. ... IPR006187. Claudin. IPR003548. Claudin1. IPR017974. Claudin_CS. IPR004031. PMP22/EMP/MP20/Claudin. ...
... the presence of claudin 1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to ... the presence of claudin 1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to ... and RAs stained positive with anti-claudin 1 antibodies. Since it has been shown that ameloblasts repeatedly alternate between ... and RAs stained positive with anti-claudin 1 antibodies. Since it has been shown that ameloblasts repeatedly alternate between ...
  • A closer examination of isolated tight junctions uncovered two related ~22 kDa, four-transmembrane domain proteins, claudin-1 and claudin-2, with no similarity to occludin. (thermofisher.com)
  • Excitement in the tight junction field continues to rise following the recent discovery of claudins -3, -4, -5, -6, -7, and -8 and experiments suggesting that tight junctions in different tissues are comprised of different sets of claudin family proteins. (thermofisher.com)
  • The alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate receptor trafficking regulator "stargazin" is related to the claudin family of proteins b. (nih.gov)
  • Stargazin is related to the claudin family of tight junction proteins. (nih.gov)
  • Besides its role in barrier function, claudin-19 regulates the expression of RPE signature genes 16 , 17 that include visual cycle proteins and retinal neurotrophic factors. (nature.com)
  • As such, cancer expression of claudin proteins is markedly dysregulated, making it an attractive target for molecular imaging like positron emission tomography (PET). (mdpi.com)
  • Cell lysates from Huh7.5.1 cells were incubated with purified GST-claudin-1 tail or GST, and proteins were pulled down with glutathione-Sepharose beads. (asm.org)
  • Candidate claudin-1 tail-interacting proteins were the GST-claudin-1-tail interactome. (asm.org)
  • To this end, MDCK C7 cells were stably transfected with either claudin-2 or claudin-10b, two paracellular cation-channel-forming TJ proteins that are not endogenously expressed in this cell line. (biologists.org)
  • Perturbation was overexpression of either claudin-2 or claudin-10b, as both form cation channels within the TJ of MDCK C7 cells, a tight renal epithelial cell line that lacks endogenous expression of these TJ proteins. (biologists.org)
  • Specific role of tight junction proteins claudin-5, occludin, and ZO-1 of the blood-brain barrier in a focal cerebral ischemic insult. (qxmd.com)
  • This study was performed to determine whether BK opens the BTB by affecting the tight junction (TJ)-associated proteins zonula occluden-1 (ZO-1), occludin, and caludin-5 and cytoskeleton protein filamentous actin (F-actin). (qxmd.com)
  • Cell entry of HCV2 and other pathogens(3-5) is mediated by tight junction (TJ) proteins, but successful therapeutic targeting of TJ proteins has not been reported yet. (ugent.be)
  • Claudin-low breast cancers are characterized by low expression levels of cell-cell adhesion molecules including E-cadherin and several of the tight junction claudin proteins, claudin 3, 4, and 7. (biomedcentral.com)
  • Clone REA751 recognizes the human claudin-3 antigen, an integral membrane protein which is a component of the claudin family of epithelial tight junction proteins. (miltenyibiotec.com)
  • These genes encode the tight junction proteins claudin-16 and claudin-19, respectively, which regulate the paracellular ion reabsortion in the kidney. (plos.org)
  • Recent evidence indicates that both exposure to HIV-1 and HIV-1 specific proteins, such as Tat protein, can contribute to alterations of expression and distribution of claudin-5 in brain endothelial cells and brain microvessels. (elsevier.com)
  • The vasodilation neuropeptides, the expressions of substance P (SP) and its preferred binding neurokinin-1 receptor (NK-1R) as well as claudin-5 of tight-junction proteins associated with BBB impairment were also assessed by Western blotting and reverse-transcriptase polymerase chain reaction. (elsevier.com)
  • By contrast, in MDCK I cells, PMA treatment resulted in redistribution of claudins 1, 3, 4 and 5 from the TJ and in reorganization of the proteins into more insoluble complexes. (deepdyve.com)
  • Tight junction proteins claudin-2 and -12 are critical for vitamin D-dependent Ca2+ absorption between enterocytes. (semanticscholar.org)
  • While tight junctions require the coordinated activity of several different proteins, the specificity of tight junction's permeability is regulated by transmembrane proteins known as claudins [ 5 ]. (biomedcentral.com)
  • Several classes of claudin interact with other proteins to form tight junction and regulate permeability of TJs. (biomedcentral.com)
  • It is also observed that the expression of claudin proteins found to be differentially regulated in several cancers. (biomedcentral.com)
  • However, there are members of the Claudin protein family whose functions are still shrouded in mystery - and if we're going to properly understand how to target blood vessels to treat cancer, we need to understand the proteins involved. (cancerresearchuk.org)
  • Yet in comparison to Medigeshi et al [5] we noticed that capsid proteins expression alone didn't bring about degradation of restricted junction essential membrane protein. (exposed-skin-care.net)
  • However just because a huge pool from the WNV capsid proteins is geared to the nuclei of contaminated cells [9] [10] transcription of claudin-1 and JAM-1 genes may be suffering from WNV replication. (exposed-skin-care.net)
  • Data in Number 2 show that WNV illness does not decrease the levels of claudin-1- or JAM-1-specific or additional mRNAs that encode limited junction proteins such as claudin-3 claudin-4 ZO-1 and occludin. (exposed-skin-care.net)
  • Recent investigations have focused on tight junction proteins, belonging to the claudin family (claudins-1 to -27 in humans) and the newly defined group of TAMP (three proteins: occludin, Marvel-D2, and tricellulin). (wiley.com)
  • This antibody reacts specifically with the ~ 22-24 kDa endogenous Claudin-5 protein. (thermofisher.com)
  • For example, claudin-5 is an essential TJ component protein ( 14 - 16 ) and its assembly is dependent on AJ-associated vascular endothelial (VE)-cadherin ( 17 ). (diabetesjournals.org)
  • In turn, claudin-5 becomes anchored to the actin cytoskeleton via zonula occludens protein-1 (ZO-1), resulting in its stable expression on cell membranes ( 14 ). (diabetesjournals.org)
  • 5. Check membrane for transfer of control protein ladder. (novusbio.com)
  • This mechanistic study provides a greater understanding of the intracellular signaling pathways mediating tight junction protein expression and supports a hypothesis that two independent pathways triggered by PI3K mediate early and late loss of paracellular claudin-5 expression. (aspetjournals.org)
  • Claudin-5 (CLDN-5), a tetra-transmembrane protein is a key component of the TJ seal that prevents the paracellular diffusion of drugs into the CNS. (aspetjournals.org)
  • We show that the EMT transcription factor Snail elicits collective migration in squamous cell carcinoma by inducing the expression of a tight junctional protein, claudin-11. (nature.com)
  • In our previous work, we have demonstrated that the blood-retinal barrier (BRB) is established in the hyaloid vasculature of zebrafish at 3 days postfertilization (dpf), and knock-down of claudin 5a gene results in leakage of plasma protein from the hyaloid vessels. (arvojournals.org)
  • Here, we demonstrated that knockout of Cldn18, a claudin family member highly expressed in lung alveolar epithelium, leads to lung enlargement, parenchymal expansion, increased abundance and proliferation of known distal lung progenitors, the alveolar epithelial type II (AT2) cells, activation of Yes-associated protein (YAP), increased organ size, and tumorigenesis in mice. (jci.org)
  • Immunologic and chemical targeting of the tight-junction protein Claudin-6 eliminates tumorigenic human pluripotent stem cells. (nih.gov)
  • Loss and gain of IL-10RA expression directly correlates with IEC barrier formation and butyrate represses permeability-promoting claudin-2 tight-junction protein expression through an IL-10RA-dependent mechanism. (jimmunol.org)
  • Claudin-1 is a tight junction transmembrane protein. (frontiersin.org)
  • Induction of claudin-4 by indomethacin was confirmed at both mRNA and protein levels. (aacrjournals.org)
  • This same OGD treatment also led to rapid degradation of tight junction protein occludin and dissociation of claudin-5 from the cytoskeleton, which contributed to OGD-induced endothelial barrier disruption. (qxmd.com)
  • Moreover, occludin protein loss and claudin-5 redistribution were detected in ischemic cerebromicrovessels. (qxmd.com)
  • In rat brain glioma model and BTB model in vitro, we find that the protein expression levels of ZO-1, occludin, and claudin-5 are attenuated by BK induction. (qxmd.com)
  • Claudin-11 (CLDN11) is a potential tumor suppressor gene located at chromosome 3 (3q26.2), and encodes a member of the claudin family, an integral membrane protein of the paracellular tight junction (TJ) [ 12 , 13 ]. (pubmedcentralcanada.ca)
  • Using a human liver-chimeric mouse model(6), we show that a monoclonal antibody specific for the TJ protein claudin-1 (ref. 7) eliminates chronic HCV infection without detectable toxicity. (ugent.be)
  • Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. (acris-antibodies.com)
  • At the blood-brain barrier (BBB), claudin (Cldn)-5 is thought to be the dominant tight junction (TJ) protein, with minor contributions from Cldn3 and -12, and occludin. (mdc-berlin.de)
  • 1999) Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. (miltenyibiotec.com)
  • Various genes show associations with schizophrenia and a very weak nominal association with the tight junction protein, claudin-5, has previously been identified. (shu.ac.uk)
  • Zusätzlich bieten wir Ihnen Claudin 18 Kits (17) und Claudin 18 Proteine (4) und viele weitere Produktgruppen zu diesem Protein an. (antikoerper-online.de)
  • whereas markedly elevated SP protein and NK-1R mRNA expressions concomitant with enhanced claudin-5 expression seemed to be associated with persistent BBB impairment in this experimental CT model. (elsevier.com)
  • Hypoxia (1% O 2 ) altered the location of claudin-5 in the plasma membrane and the level of claudin-5 protein in bEND.3 cells, and these changes were accompanied by a decrease in the transendothelial electrical resistance. (elsevier.com)
  • Claudin 5 is a tight junction protein required for blood brain barrier (BBB) and, probably, choroid plexus (CP) structure and function in vertebrates. (biologists.org)
  • Guanine nucleotide-binding protein Gαi2: a new partner of claudin-5 that regulates tight junction integrity in human brain endothelial cells. (crcm-marseille.fr)
  • The transmembrane protein claudin-5 is known as a key TJ protein at the BBB, although, the molecular mechanisms by which it regulates TJ tightness are poorly understood. (crcm-marseille.fr)
  • In MDCK II cells, barrier improvement by claudin-8 is accompanied by a reduction of endogenous claudin-2 protein at the tight junction. (deepdyve.com)
  • CLDN18 (Claudin 18) is a Protein Coding gene. (genecards.org)
  • So far the only other clue came from research linking higher levels of Claudin-14 protein to the shape endothelial cells grew in. (cancerresearchuk.org)
  • Claudin-4 (Clostridium perfringens enterotoxin receptor) is a tight junction protein encoded by the gene CLDN4. (biocare.net)
  • In luminal breast cancer, the increase of Claudin-4 protein was correlated with the increase of tumor grade and with Ki-67, and thus demonstrated an overall shorter life survival (3). (biocare.net)
  • Low expression of the Claudin-4 protein correlated with higher T staging, lymphatic metastasis and higher risk of recurrence in esophageal squamous cell carcinoma (6). (biocare.net)
  • Loss of the tight junction protein claudin 4 correlates with histological growth-pattern and differentiation in advanced gastric adenocarcinoma. (biocare.net)
  • This method involves using inexpensive peptides (protein fragments) that bind to a protein called claudin 5. (alz.org)
  • Claudin 5 helps form protein complexes called "tight junctions" that regulate transportation across the blood-brain barrier. (alz.org)
  • The goal of this study is to examine claudin-1 expression in a tissue microarray of colorectal cancer and metastases, simultaneously, in order to assess the prognostic value of this protein in colorectal cancer. (oatext.com)
  • This is the first study to demonstrate the expression of claudin-1 protein across a sample of colorectal cancer and its metastases, simultaneously. (oatext.com)
  • degradation of claudin protein in lysosomes [5]. (exposed-skin-care.net)
  • Data in Amount 1 present that in every cases the restricted junction membrane protein claudin-1 and JAM-1 are degraded in WNV contaminated cells. (exposed-skin-care.net)
  • Amount 1 WNV an infection results in lack of claudin-1 and JAM-1 protein in epithelial and endothelial cells. (exposed-skin-care.net)
  • Astragaloside A Dynamin and microtubules are necessary for WNV-induced degradation of claudin-1 and JAM-1 Having eliminated the chance that WNV an infection impacts the transcription and/or degradation of restricted junction protein-encoding mRNAs we following focused on identifying how virus an infection induces degradation of claudin-1 and JAM-1 protein. (exposed-skin-care.net)
  • VEGF overexpression in the mouse retina caused delayed inflammatory cytokine upregulation, monocyte/macrophage infiltration (CD11b + Ly6C + CCR2 + cells), macrophage/microglia activation (CD11b + CD80 + cells), and blood-retinal barrier disruption due to claudin-5 redistribution, which did not recover with VEGF blockade alone. (diabetesjournals.org)
  • The following product was used in this experiment: Claudin 5 Monoclonal Antibody (4C3C2) from Thermo Fisher Scientific, catalog # 35-2500, RRID AB_2533200. (thermofisher.com)
  • 2. Incubate primary antibody overnight or at room temperature for 2 hours prepared in 5% milk/TBST. (novusbio.com)
  • 3. Remove antibody solution and wash membrane three times for 5-15 minutes in TBST. (novusbio.com)
  • 4. Incubate membrane with an appropriate secondary antibody (peroxidase conjugated) for 1 hour at room temperature in 5% milk/TBST. (novusbio.com)
  • Unexpectedly, the anti-claudin-1 antibody localized claudin to the Golgi apparatus of a sub-population of SA and RA in addition to the distal junctions of RA. (frontiersin.org)
  • The lysates were immunoprecipitated with anti-claudin-1 antibody followed by Western blotting. (asm.org)
  • Immunohistochemistry (IHC) analyzes of Claudin-1 antibody (Cat. (acris-antibodies.com)
  • Western blot (WB) analysis of Claudin-1 antibody (Cat. (acris-antibodies.com)
  • In 25 liver biopsy samples taken from dogs with portal vein hypoperfusion, an increased number of arterioles was detected in the portal areas (PAs) by the use of humanised anti-claudin-5 antibody. (mtak.hu)
  • The modulating agents comprise at least one claudin cell adhesion recognition sequence or an antibody or fragment thereof that specifically binds the claudin cell adhesion recognition sequence. (justia.com)
  • The present invention relates generally to methods for regulating claudin-mediated processes, and more particularly to the use of modulating agents comprising a claudin cell adhesion recognition sequence and/or an antibody that specifically recognizes such a sequence for inhibiting functions such as cell adhesion and the formation of tissue permeability barriers. (justia.com)
  • Overexpression of snail correlates with increased claudin-11, and both are associated with a worse outcome. (nature.com)
  • Overexpression of claudin-4 in AGS cells did not affect cell growth or the induction of apoptosis by indomethacin. (aacrjournals.org)
  • On the other hand, addition of indomethacin or overexpression of claudin-4 inhibited cell migration. (aacrjournals.org)
  • Our findings demonstrate that claudin overexpression studies measure the combined effect of alterations in both endogenous and exogenous claudins, thus explaining the dependence of the phenotype on the host cell line. (deepdyve.com)
  • Claudin-6, 7, or 9 overexpression in the human gastric adenocarcinoma cell line AGS increases its invasiveness, migration, and proliferation rate. (semanticscholar.org)
  • Claudin-4 overexpression was able to independently predict survival in a breast cancer multivariate analysis as it was associated with poor prognosis, high tumor grade and Her2 expression and was inversely correlated with estrogen receptor staining (2). (biocare.net)
  • Basal-like tumors also demonstrated overexpression of Claudin-4 (4). (biocare.net)
  • Claudin-4 overexpression in prostate cancer may offer a Claudin-4 targeted therapy as a potential treatment (7). (biocare.net)
  • This gene encodes a member of the claudin family. (thermofisher.com)
  • The claudin 5b gene has positive and negative regulatory elements in the 5' flanking region. (arvojournals.org)
  • This gene encodes a component of tight junction strands, which is a member of the claudin family. (nih.gov)
  • However, the effects of microRNA-1303 (miR-1303) on gastric cancer (GC) cells and the upstream regulation of GC-associated claudin-18 gene (CLDN18) remain unclear. (springer.com)
  • The claudin gene family: expression in normal and neoplastic tissues. (springer.com)
  • In breast cancer, gene expression analyses have defined five tumor subtypes (luminal A, luminal B, HER2-enriched, basal-like and claudin-low), each of which has unique biologic and prognostic features. (nih.gov)
  • Furthermore, schizophrenia occurs in 30% of individuals with 22q11 deletion syndrome (22q11DS), a population who are haploinsufficient for the claudin-5 gene. (shu.ac.uk)
  • Here, we show that a variant in the claudin-5 gene is weakly associated with schizophrenia in 22q11DS, leading to 75% less claudin-5 being expressed in endothelial cells. (shu.ac.uk)
  • In 1999, Simon and colleagues identified mutations in the gene PCLN1, subsequently called CLDN16 , as the underlying cause of FHHNC [5] . (plos.org)
  • Here, we show that the gene claudin 5a is the zebrafish orthologue with high fidelity expression, in the BBB and CP barriers, that demonstrates the conservation of the BBB and CP between humans and zebrafish. (biologists.org)
  • Here, we show that this is not because of relocalization of claudin-2 into the cytosolic pool but primarily due to a decrease in gene expression. (deepdyve.com)
  • Recently, an additional subtype has been identified [ 7 ], the claudin-low subtype, which, based on gene its expression profile, is characterized by low expression of luminal markers and high expression of mesenchymal markers. (biomedcentral.com)
  • Gene expression profile studies have identified multiple subtypes of TNBC, including basal-like and claudin-low/mesenchymal-like. (springer.com)
  • Claudin-4 is the first to confer ionic selectivity to paracellular transport, leading to the prediction that the combination of different claudins defines the overall selectivity of different junctions. (thermofisher.com)
  • Because stargazin is structurally similar to claudins, we hypothesized that it might also have retained claudin-like functions inherited from a common ancestor. (nih.gov)
  • The highly conserved residues Y148, Y158 and E159 in ECL2 of claudin-5 contribute to homo- and/or heterophilic trans-interaction between classic claudins and thereby tighten the paracellular space against ions, small and large molecules. (nih.gov)
  • In HT29 cells, claudins 1, 3, 4 and 5 and possibly claudin 2 were redistributed to apical cell-cell contacts after PKC activation and the amounts of claudins 1, 3 and 5, but not of claudin 2, were increased in cell lysates. (deepdyve.com)
  • Claudins 1 and 4 were phosphorylated in both MDCK I and HT29 cells, but PKC-induced changes in claudin phosphorylation state were detected only in MDCK I cells. (deepdyve.com)
  • Unlike in MDCK II cells, induction of claudin-8 in MDCK I cells (which did not affect levels of endogenous claudins) did not alter paracellular ion permeability. (deepdyve.com)
  • A prime example of the aforementioned role of claudins in carcinogenesis and metastases is claudin-1. (oatext.com)
  • Claudin-1 along with claudin-3 and claudin-5 are shown to promote pro-MMP2 whereby claudins recruit MMPs on the cell surface to achieve elevated focal concentrations and eventual activations of pro-MMP2 [12]. (oatext.com)
  • Here, we analyzed the impact of residues in the second extracellular loop (ECL2) of mouse claudin-5 on paracellular permeability. (nih.gov)
  • Expression of claudin-5(Y148A), (Y158A) or (E159Q) enhanced permeability of FITC-dextran(10 kDa), which was unchanged in cells expressing claudin-5(wild type) or claudin-5(R145A). (nih.gov)
  • To evaluate endothelial cell permeability after metformin treatment, we conducted occludin/CD31, ZO-1/CD31 and claudin-5/CD31 double staining to observe tight junction distribution in situ at 3 days after tMCAO. (nih.gov)
  • This study aimed to test the hypothesis that transforming growth factor (TGF)-β1 increases the paracellular permeability of vascular endothelial monolayers through tyrosine phosphorylation of VE-cadherin and claudin-5. (open.ac.uk)
  • Our results suggest that tyrosine phosphorylation of VE-cadherin and claudin-5 is involved in the increased paracellular permeability of central nervous system-derived vascular endothelium induced by TGF-β1. (open.ac.uk)
  • While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. (uniprot.org)
  • Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. (uniprot.org)
  • Under all conditions, water flux in claudin-2-transfected cells was elevated compared with vector controls, indicating claudin-2-mediated paracellular water permeability. (biologists.org)
  • This study demonstrates that the increase of BK-mediated BTB permeability is associated with the down-regulation of ZO-1, occludin, and claudin-5 and the rearrangement of F-actin and that cAMP/PKA signal transduction system might be involved in the modulating process. (qxmd.com)
  • Members of the claudin family are known to determine tight junction permeability in a charge- and size-selective manner. (rupress.org)
  • Degradation of claudin-5 coincided with alteration of the blood-CSF barrier permeability and treatment with the MMP inhibitor GM6001 attenuated the degradation of claudin-5. (duhnnae.com)
  • A claudin subtype that is associated with the formation of cation-selective channels and increased epithelial permeability. (semanticscholar.org)
  • Moreover, small interfering RNA-mediated depletion of Gαi2 or claudin-5 in hCMEC/D3 cells similarly increases their paracellular permeability and delays TJ recovery after mannitol treatment. (crcm-marseille.fr)
  • We previously reported that claudin-8 reduces paracellular cation permeability when expressed in low-resistance Madin-Darby canine kidney (MDCK) II cells. (deepdyve.com)
  • Furthermore, when endogenous claudin-2 in MDCK II cells was downregulated by epidermal growth factor to create a cell model with low transepithelial resistance and low levels of claudin-2, the permeability effects of claudin-8 were also abolished. (deepdyve.com)
  • In contrast to occludin, which induces only a small number of short strands at cell-cell contact sites when introduced into fibroblasts lacking tight junctions, claudin-1 and -2 induce networks of strands characteristic of true tight junctions. (thermofisher.com)
  • Though inconclusive, these findings suggest that claudin-1 and -2 are major structural components of TJ strands and that occludin plays some other accessory role. (thermofisher.com)
  • Metformin promoted ZO-1, occludin and claudin-5 rearrangement and lessened IgG and Evans blue extravasation. (nih.gov)
  • A) Occludin, ZO-1 and claudin-5 expression in sham group, NS and metformin-treated mice at 3 days following tMCAO. (nih.gov)
  • B) Representative result of occludin, ZO-1 and claudin-5 expression at 1 and 3 days after tMCAO. (nih.gov)
  • Bar graphs show a quantification of occludin, ZO-1 and claudin-5 expression. (nih.gov)
  • Result indicated that occludin and ZO-1 were continuously located on the margin of ECs in sham group, claudin-5 was continuously located along ECs, and fewer gaps were formed in the metformin-treated group (Figure 4A). (nih.gov)
  • Among identified genes, we focused on those related to tight junction function ( claudin-4 , claudin-1 , and occludin ), particularly claudin-4 . (aacrjournals.org)
  • C) Cell lysates from Huh7.5.1 cells were pulled down by GST-CD81-N-tail, GST-CD81-C-tail, GST-occludin-tail, GST-SRB1-tail, GST-claudin-1 tail or GST with two extra washes. (asm.org)
  • Using selective MMP-2/9 inhibitor SB-3CT (2-[[(4-phenoxyphenyl)sulfonyl]methyl]-thiirane) or their neutralizing antibodies or Cav-1 siRNA, we found that MMP-2 was the major enzyme mediating OGD-induced occludin degradation, while Cav-1 was responsible for claudin-5 redistribution. (qxmd.com)
  • These data indicate that cerebral ischemia initiates two rapid parallel processes, MMP-2-mediated occludin degradation and Cav-1-mediated claudin-5 redistribution, to cause BBB disruption at early stroke stages relevant to acute thrombolysis. (qxmd.com)
  • The redistribution of ZO-1, occludin, and claudin-5 and rearrangement of F-actin in brain microvascular endothelial cells are observed at the same time. (qxmd.com)
  • RT-PCR and Western blot analysis showed an increase in the abundance of claudin-3 and an apparent change in the posttranslational modification of occludin, data reinforcing a paracellular barrier alteration. (scialert.net)
  • The objectives of this study were to investigate the localization of tight junctions and the modulation of zonula occludens- (ZO-) 1, occludin and claudin-1 expression by estrogen in castrated female rat vagina. (nih.gov)
  • The cellular localization and expression of ZO-1, occludin, and claudin-1 were determined in each group by immunohistochemistry and western blot. (nih.gov)
  • Expression of ZO-1, occludin, and claudin-1 was significantly decreased after ovariectomy and was restored to the level of the control after estrogen replacement. (nih.gov)
  • To identify putative claudin-5 partners that contribute to TJ integrity, claudin-5-enriched membrane microdomains were prepared by cell fractionation, using the human brain endothelial cell line hCMEC/D3 and claudin-5 immunoprecipitates were submitted to tandem mass spectrometry. (crcm-marseille.fr)
  • Interestingly, we show that a group of highly utilized breast cancer cell lines, and several genetically engineered mouse models, express the claudin-low phenotype. (nih.gov)
  • Using an inducible 'knockdown' mouse model, we further link claudin-5 suppression with psychosis through a distinct behavioural phenotype showing impairments in learning and memory, anxiety-like behaviour and sensorimotor gating. (shu.ac.uk)
  • Tracer experiments revealed that the barrier function of hypoxic retinal vasculature with depressed claudin-5 expression was selectively disrupted against small molecules, which is very similar to the phenotype of claudin-5-deficient mice. (elsevier.com)
  • To test whether replacement of cation-permeable claudin-2 mediates the effect of claudin-8 on the electrophysiological phenotype of the host cell line, we expressed claudin-8 in high-resistance MDCK I cells, which lack endogenous claudin-2. (deepdyve.com)
  • The purpose of this study is to identify DNA regulatory sequences of the zebrafish claudin 5 genes. (arvojournals.org)
  • Exogenous expression of wild type, but not mutant claudin-19, increases the expression of RPE signature genes. (nature.com)
  • In mammals, humans have 23 claudin genes, whereas rats and mice have 24 genes ( Lal-Nag and Morin, 2009 ). (frontiersin.org)
  • Claudin-low tumors are characterized by the low to absent expression of luminal differentiation markers, high enrichment for epithelial-to-mesenchymal transition markers, immune response genes and cancer stem cell-like features. (nih.gov)
  • Based on these results, we consider that the induction of claudin-4 and other tight junction-related genes by NSAIDs may be involved in the chemopreventive effect of NSAIDs through the suppression of anchorage-independent growth and cell migration. (aacrjournals.org)
  • Here, we use molecular dynamics simulations to build and refine an atomic model of claudin-15 channels and study its transport properties. (rupress.org)
  • The pivotal role of EMT in hepatocellular carcinoma (HCC) has been increasingly recognized and various molecular mechanisms of hepatocellular EMT have been identified ( 4, 5 ). (aacrjournals.org)
  • Claudin expression in high-grade invasive ductal carcinoma of the breast: correlation with the molecular subtype. (biocare.net)
  • Ripasudil also contributed to the stability of claudin-5 expression by both transcriptional enhancement and degradation suppression in inflammatory cytokine-stimulated endothelium. (diabetesjournals.org)
  • This might involve rapid activation of metalloproteinase, a disintegrin and metalloproteinase 17 (ADAM17), and ADAM17-mediated claudin-5 degradation. (biomedcentral.com)
  • These results suggested that degradation of claudin-5 was caused by MMP-9 in angiostrongyliasis meningoencephalitis. (duhnnae.com)
  • a) Immunohistochemistry of claudin-1 in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy plus 17β-estradiol treatment (Ovx + Est) groups. (nih.gov)
  • Claudin-4 immunohistochemistry is highly effective in distinguishing adenocarcinoma from malignant mesothelioma in effusion cytology. (biocare.net)
  • This study analysed the expression of claudin-1 by immunohistochemistry in 99 tissue samples (including 33 colorectal cancer cases with 33 concordant lymph node metastases and 33 concordant cancer-adjacent normal tissues, all from the same patients). (oatext.com)
  • In mice, claudin-19 was found in the kidney and Schwan cells of the peripheral nervous system, but was absent from the oligodendrocytes of the central nervous system 4 . (nature.com)
  • After virotherapy with claudin-5, the cardiac contractile force deficits in Sgcd −/− mice are no longer significant. (frontiersin.org)
  • Because TJs of basal cells are primarily composed of claudin-11, claudin-11-deficient (Cld11 -/- ) mice were generated with an expectation that the compartmentalization in stria vascularis in these mice would be affected. (biologists.org)
  • Mapping of Sensory Nerve Subsets within the Vagal Ganglia and the Brainstem Using Reporter Mice for Pirt, TRPV1, 5-HT3, and Tac1 Expression. (usf.edu)
  • Our findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2. (jimmunol.org)
  • Claudin-5 is expressed in endothelial cells forming part of the blood-brain barrier (BBB). (shu.ac.uk)
  • The presented data substantiate the hypothesis that claudin-18 is a central barrier-forming component of tight junctions and show that IL-13 (zeige IL13 Antikörper ) downregulates claudin-18 . (antikoerper-online.de)
  • Andras, IE & Toborek, MJ 2011, HIV-1-induced alterations of claudin-5 expression at the blood-brain barrier level . (elsevier.com)
  • The mechanisms underlying the hypoxia-induced disruption of the barrier function of neural vasculature were analyzed with reference to the expression of claudin-5, a component of tight junctions between neural endothelial cells. (elsevier.com)
  • Inhibition of the expression of claudin-5 by RNAi resulted in a reduction of transendothelial electrical resistance, indicating a critical role of claudin-5 in the barrier property. (elsevier.com)
  • These in vitro and in vivo data indicate that claudin-5 is a target molecule of hypoxia leading to the disruption of the barrier function of neural vasculature. (elsevier.com)
  • Claudin-5 could be used for the pathophysiologic evaluation of the blood-CSF barrier breakdown and tight junction disruption after infection with A. cantonensis. (duhnnae.com)
  • Here, we address how the interaction of heterologously expressed claudin-8 with endogenous claudin isoforms impacts epithelial barrier properties. (deepdyve.com)
  • We recently showed in a renal epithelial cell culture system that reduction of culture medium methionine by 80% resulted in altered tight junctional (TJ) claudin composition and also improved epithelial barrier function ( 51 ). (scialert.net)
  • AKT2 maintains brain endothelial claudin-5 expression and selective activation of IR/AKT2/FOXO1-signaling reverses barrier dysfunction. (usf.edu)
  • By binding to claudin 5, the peptides should be able to create a temporary gap in the blood-brain barrier through which drug compounds could enter the brain. (alz.org)
  • High GLI1 expression is a property of claudin-low cells and tumors and correlates with markers of EMT and breast cancer stem cells. (biomedcentral.com)
  • Expression of claudin 5a correlates with developmental tightening of the BBB and is restricted to a subset of the brain vasculature clearly delineating the BBB. (biologists.org)
  • The blocking solution is normally composed of 5% non-fat milk in TBS-T, and some antibodies require BSA in place of milk. (novusbio.com)
  • In the present study, to investigate whether CLDN-5 binders can be used for delivery of drugs to the CNS, we generated monoclonal antibodies (mAbs) specific to the extracellular domains of CLDN-5. (aspetjournals.org)
  • Distal junction area of ruffle-ended ameloblasts (RA) and the Golgi apparatus of a sub-population of smooth-ended ameloblasts (SA) and RAs stained positive with anti-claudin-1 antibodies. (frontiersin.org)
  • B) Huh7.5 cells treated with Sec24C siRNA or a control siRNA for 72 h were stained with antibodies against claudin-1 (green) and the tight junction marker ZO-1 (red). (asm.org)
  • A) Huh7.5 cells treated with different concentrations of XN for 12 h were fixed with methanol and stained with antibodies against claudin-1 (red) and the tight junction marker ZO-1 (green). (asm.org)
  • PI3K inhibitors LY294002 (2.5-25 μM) and PI-828 (0.1-10 μM) prevented the 3-chloropropanediol-induced AKT (Thr308) phosphorylation and both early and late loss of paracellular claudin-5. (aspetjournals.org)
  • Tyrosine phosphorylation of VE-cadherin and claudin-5 was studied by ICC, immunoprecipitation and Western blotting. (open.ac.uk)
  • ICC revealed that tyrosine phosphorylation of endothelial monolayers was greatly enhanced by TGF-β1 treatment, and immunoprecipitation of cell lysates showed increased tyrosine phosphorylation of VE-cadherin and claudin-5. (open.ac.uk)
  • Finally, we show that anti-psychotic medications dose-dependently increase claudin-5 expression in vitro and in vivo while aberrant, discontinuous expression of claudin-5 in the brains of schizophrenic patients post mortem was observed compared to age-matched controls. (shu.ac.uk)
  • miR-1303 could bind to the putative binding sites in CLDN18 mRNA 3′-UTR and visibly lower the expression of claudin-18. (springer.com)
  • Auf www.antikoerper-online.de finden Sie aktuell 60 Claudin 18 (CLDN18) Antikörper von 14 unterschiedlichen Herstellern. (antikoerper-online.de)
  • miR-1303 was significantly overexpressed whereas claudin-18 was downregulated in GC tissues and cell lines, which was significantly associated with tumor size, location invasion, histologic type and tumor-node-metastasis stage. (springer.com)
  • Here, we comprehensively characterize the recently identified claudin-low tumor subtype. (nih.gov)
  • The clinical, pathological and biological features of claudin-low tumors were compared to the other tumor subtypes using an updated human tumor database and multiple independent data sets. (nih.gov)
  • Knockdown of GLI1 expression in claudin-low cell lines resulted in reduced cell viability, motility, clonogenicity, self-renewal, and reduced tumor growth of orthotopic xenografts. (biomedcentral.com)
  • Paget hypothesized that the interaction between tumor cells (seed) and host environment (soil) determines metastatic outcome ( 5 ). (spandidos-publications.com)
  • Claudin 6 expression is useful to distinguish myxofibrosarcomas from other myxoid soft tissue tumors. (nih.gov)
  • These main features of claudin-low tumors were also evaluated in a panel of breast cancer cell lines and genetically engineered mouse models. (nih.gov)
  • Clinically, the majority of claudin-low tumors are poor prognosis estrogen receptor (ER)-negative, progesterone receptor (PR)-negative, and epidermal growth factor receptor 2 (HER2)-negative (triple negative) invasive ductal carcinomas with a high frequency of metaplastic and medullary differentiation. (nih.gov)
  • These results should help to improve our understanding of the biologic heterogeneity of breast cancer and provide tools for the further evaluation of the unique biology of claudin-low tumors and cell lines. (nih.gov)
  • Claudin-low tumors are mainly triple-negative invasive ductal carcinomas with a high frequency of metaplastic and medullary differentiation. (biomedcentral.com)
  • Finally, we confirm that a prognostically relevant differentiation hierarchy exists across all breast cancers in which the claudin-low subtype most closely resembles the mammary epithelial stem cell. (nih.gov)
  • Loss of Claudin-4 was also seen in 69% of advanced gastric cancers and correlated with poor differentiation (5). (biocare.net)
  • Claudin-1 expression is frequently altered in several cancers, including upregulation and down regulation [13]. (oatext.com)
  • If using nitrocellulose, sample transfer and relative concentration can be assessed by staining the membrane with Ponseau S solution (0.1% ponceau, 5% acetic acid). (novusbio.com)
  • These typically take the form of two solutions which are combined and then incubated immediately on the membrane for 1 - 5 mintues. (novusbio.com)
  • Functional studies suggest a dual role for stargazin, both as a modulatory gamma subunit for voltage-dependent calcium channels and as a regulator of post-synaptic membrane targeting for alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate (AMPA)-type glutamate receptors. (nih.gov)
  • The movement of claudin-5 from the cytoplasm to the plasma membrane of cultured confluent brain-derived endothelial (bEND.3) cells was closely correlated with the increase in the transendothelial electrical resistance. (elsevier.com)
  • In vivo the claudin-5 molecules were expressed under normoxia in the plasma membrane of retinal microvascular endothelial cells but were significantly reduced under hypoxic conditions. (elsevier.com)
  • Administration of ripasudil, a selective ROCK inhibitor, attenuated retinal inflammation and claudin-5 redistribution. (diabetesjournals.org)
  • Mutated claudin-19 affects multiple stages of RPE and retinal differentiation through its effects on multiple functions of the RPE. (nature.com)
  • F) Coimmunoprecipitation of endogenous claudin-1 with Sec24C in Huh7.5.1 cells. (asm.org)
  • The recently identified claudin-low subtype of breast cancer is enriched for cells with stem-like and mesenchymal-like characteristics. (biomedcentral.com)
  • Thus, Claudin-4 forms channels through the tight junctions that discriminate against Na+ ions and are indifferent to Cl- ions. (thermofisher.com)
  • Mechanistically, tyrosine-phosphorylated claudin-11 activates Src, which suppresses RhoA activity at intercellular junctions through p190RhoGAP, maintaining stable cell-cell contacts. (nature.com)
  • Claudin-11/OSP-based tight junctions of myelin sheaths in brain and Sertoli cells in testis. (jax.org)
  • Claudin-8 also affects the trafficking of claudin-2, which was displaced specifically from the junctions at which claudin-8 was inserted. (deepdyve.com)
  • The claudin-1 assembly with tight junctions is regulated by post translational modifications. (biomedcentral.com)
  • and Thr 191 may provide an on/off switch to regulate assembly of claudin-1 at tight junctions. (biomedcentral.com)
  • The type of claudin you find in the junctions depends on what type of cell you look at, and until recently it was thought that Claudin-5 was the main Claudin sticking our endothelial cells together. (cancerresearchuk.org)
  • Stable expression of claudin-5(wild type) in MDCK-II cells-but not that of mutants R145A, Y148A, Y158A or E159Q-increased transepithelial electrical resistance and decreased fluorescein permeation. (nih.gov)
  • Title: TLR2 activation induced by H. pylori LPS promotes the differential expression of claudin-4, -6, -7 and -9 via either STAT3 and ERK1/2 in AGS cells. (nih.gov)
  • Suppression of claudin-4 expression by small interfering RNA restored the migration activity of AGS cells in the presence of indomethacin. (aacrjournals.org)
  • Cell lysates from Huh7.5.1 cells were pulled down by GST-claudin-1 tail or GST followed by Western blotting. (asm.org)
  • D and E) Immunoblots showing the association of Sec24C with claudin-1 in transfected HEK293T cells by a coimmunoprecipitation assay. (asm.org)
  • HEK293T cells were transfected with Flag-claudin-1, Flag-SRB1, or PCMV-Tag2 empty vector for 48 h. (asm.org)
  • G) Colocalization of claudin-1 with Sec24C in Huh7.5.1 cells. (asm.org)
  • Huh7.5.1 cells were fixed by PFA and immunofluorescently labeled for claudin-1 (green) and Sec24C (red). (asm.org)
  • C) Cell surface claudin-1 was quantified by FACS assay in nonpermeabilized cells. (asm.org)
  • D) Intercellular claudin-1 was quantified by FACS assay in permeabilized cells. (asm.org)
  • Bile duct adenocarcinoma cells overexpress claudin-18 via the EGFR (zeige EGFR Antikörper )/ RAS / ERK (zeige EPHB2 Antikörper ) pathway, contributing to cell proliferation and invasion. (antikoerper-online.de)
  • This paper describes that the endothelial cells of normal hepatic arterioles, portal venules and portal lymphatics as well as the endothelium of sinusoids from dogs show strong membranous claudin-5 cross-reactivity. (mtak.hu)
  • We show that claudin 5a -expressing cells of the CP are ciliated ependymal cells that drive fluid flow in the brain ventricles. (biologists.org)
  • A major difference between HT29 and MDCK I cells, which have low and high basal transepithelial electrical resistance, respectively, was the absence of claudin 2 in the latter. (deepdyve.com)
  • The samples in cluster 1 also share many features with the newly identified claudin-low breast cancer intrinsic subtype, which also shows characteristics of stromal and stem cells. (biomedcentral.com)
  • Inhibition of Src and p38 MAP kinases suppresses the change of claudin expression induced on dedifferentiation of primary cultured parotid acinar cells. (semanticscholar.org)
  • For example at 24 h post-infection claudin-1 mRNA levels Astragaloside A were >1.8 collapse higher than in mock-treated cells and at 72 h post-infection they were 3.9 times higher (p? (exposed-skin-care.net)
  • A single dose of ketamine resulted in a significant increase in the number of 5-bromo-2-deoxyuridine-positive (BrdU + ) cells in the dentate gyrus (DG) of rats at 24 h, but not at 28 days, after treatment completion. (chemweb.com)
  • We also show that targeted adeno-associated virus-mediated suppression of claudin-5 in the mouse brain results in localized BBB disruption and behavioural changes. (shu.ac.uk)
  • Indeed, interactions between the transmigrating PMN and the epithelium result in signaling events which in turn may be amplified and prolonged during high-density PMN transmigration ( 5 , 6 ). (jimmunol.org)
  • Claudin-6: a novel tight junction molecule is developmentally regulated in mouse embryonic epithelium. (semanticscholar.org)
  • Claudin-1 was most intense in the superficial layer of the vaginal epithelium in the control group. (nih.gov)
  • Claudin-1 was most intense in the superficial layer of the vaginal epithelium. (nih.gov)
  • 18 F-Labeled claudin selective peptides would provide access to a novel kind of imaging tools for pancreatic cancer. (mdpi.com)
  • These probes were prepared using oxime ligation of 5-[ 18 F]fluoro-5-deoxyribose (5-[ 18 F]FDR) to claudin selective peptides. (mdpi.com)
  • For this proposed grant, Dr. Karande and colleagues will design and test several peptides that can bind to claudin 5. (alz.org)
  • Our results uncover crosstalk between NFκB and GLI1 signals and suggest that targeting these pathways may be effective against the claudin-low breast cancer subtype. (biomedcentral.com)
  • Claudin extracellular domains determine paracellular charge selectivity and resistance but not tight junction fibril architecture. (semanticscholar.org)
  • Reactivity has been confirmed with rat, human and mouse Claudin-5 using rat lung, mouse kidney, mouse small intestine, mouse lung homogenates, human colon tissue, and CACO-2 human cell line. (thermofisher.com)
  • Changes in VE-cadherin and claudin-5 expression were studied by immunocytochemistry (ICC) and quantified by cell-based enzyme linked immunosorbent assays (ELISA). (open.ac.uk)
  • This effect was enhanced by the tyrosine phosphatase inhibitor orthovanadate and attenuated by the tyrosine kinase inhibitor lavendustin A. ICC and cell-based ELISA revealed that TGF-β1 induced both dose- and time-dependent decreases in VE-cadherin and claudin-5 expression. (open.ac.uk)
  • In head and neck cancer patients, the Snail-claudin-11 axis prompts the formation of circulating tumour cell clusters, which correlate with tumour progression. (nature.com)
  • The ARPE19 cell line fails to express claudin-19. (nature.com)
  • DNA methylation of claudin-6 promotes breast cancer cell migration and invasion by recruiting MeCP2 and deacetylating H3Ac and H4Ac. (nih.gov)
  • The expression of miR-1303 and claudin-18 in GC tissues and gastric cancer cell lines were detected by qRT-PCR and western blotting, respectively. (springer.com)
  • Knocking down Sec24C reduced the cell surface level of claudin-1. (asm.org)
  • Blocking COPII transport by XN decreased the cell surface level of claudin-1. (asm.org)
  • Most cell types express more than two claudin species in various combinations to constitute TJ strands. (biologists.org)
  • Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. (acris-antibodies.com)
  • We observed non-canonical activation of GLI1 in claudin-low and EMT cell lines, and identified crosstalk with the NFκB pathway. (biomedcentral.com)
  • We further provide direct evidence for the binding of the NFκB subunit p65 to the GLI1 promoter in both EMT and claudin-low cell lines. (biomedcentral.com)
  • The density of small tight junction pores varies among cell types and is increased by expression of claudin-2. (semanticscholar.org)
  • Methods for using modulating agents to enhance or inhibit claudin-mediated cell adhesion in a variety of in vivo and in vitro contexts are provided. (justia.com)
  • Low expression of claudin-4: an indicator of recurrence in esophageal squamous cell carcinoma after Ivor Lewis esophagectomy? (biocare.net)
  • Counter to the above breast cancer subtypes, the presence of Claudin-4 in triple negative breast cancer was a biomarker that demonstrated a favorable prognosis (3). (biocare.net)
  • Claudin molecules bear four transmembrane domains with both N- and C-termini located in the cytoplasm. (biologists.org)
  • Our simulations indicate that claudin-15 forms well-defined channels for ions and molecules and otherwise "seals" the paracellular space through hydrophobic interactions. (rupress.org)
  • The simulations suggest that the selectivity filter is formed by a cage of four aspartic acid residues (D55), contributed by four claudin-15 molecules, which creates a negative electrostatic potential to favor cation flux over anion flux. (rupress.org)
  • Jun, Kim, Jung, Choi, Chin: Expression of claudin-7 and loss of claudin-18 correlate with poor prognosis in gastric cancer. (antikoerper-online.de)
  • Expression of Claudin-4 has been associated with either poor prognosis or a more favorable diagnosis, depending on the type of cancer. (biocare.net)
  • Increased claudin-4 expression is associated with poor prognosis and high tumour grade in breast cancer. (biocare.net)
  • During HCV infection claudin-1 is highly expressed in liver and believed to be associated with HCV virus entry after HCV binding with or without co-receptor CD81. (biomedcentral.com)
  • Claudin-3 and claudin-4 mRNA levels steadily improved during WNV illness and between 48 and 72 h were as much as 2.2 (p? (exposed-skin-care.net)