Stem Cell Factor: A hematopoietic growth factor and the ligand of the cell surface c-kit protein (PROTO-ONCOGENE PROTEINS C-KIT). It is expressed during embryogenesis and is a growth factor for a number of cell types including the MAST CELLS and the MELANOCYTES in addition to the HEMATOPOIETIC STEM CELLS.Hematopoietic Cell Growth Factors: These growth factors comprise a family of hematopoietic regulators with biological specificities defined by their ability to support proliferation and differentiation of blood cells of different lineages. ERYTHROPOIETIN and the COLONY-STIMULATING FACTORS belong to this family. Some of these factors have been studied and used in the treatment of chemotherapy-induced neutropenia, myelodysplastic syndromes, and bone marrow failure syndromes.Proto-Oncogene Proteins c-kit: A protein-tyrosine kinase receptor that is specific for STEM CELL FACTOR. This interaction is crucial for the development of hematopoietic, gonadal, and pigment stem cells. Genetic mutations that disrupt the expression of PROTO-ONCOGENE PROTEINS C-KIT are associated with PIEBALDISM, while overexpression or constitutive activation of the c-kit protein-tyrosine kinase is associated with tumorigenesis.Claudin-1: An integral membrane protein that is localized to TIGHT JUNCTIONS, where it plays a role in controlling the paracellular permeability of polarized cells. Mutations in the gene for claudin-1 are associated with Neonatal Ichthyosis-Sclerosing Cholangitis (NISCH) Syndrome.Claudin-4: A claudin subtype that takes part in maintaining the barrier-forming property of TIGHT JUNCTIONS. Claudin-4 is found associated with CLAUDIN-8 in the KIDNEY COLLECTING DUCT where it may play a role in paracellular chloride ion reabsorption.Claudin-3: A ubiquitously-expressed claudin subtype that acts as a general barrier-forming protein in TIGHT JUNCTIONS. Elevated expression of claudin-3 is found in a variety of tumor cell types, suggesting its role as a therapeutic target for specific ANTINEOPLASTIC AGENTS.Claudin-5: A claudin subtype that is found localized to TIGHT JUNCTIONS in VASCULAR ENDOTHELIAL CELLS. The protein was initially identified as one of several proteins which are deleted in VELOCARDIOFACIAL SYNDROME and may play an important role in maintaining the integrity of the BLOOD-BRAIN BARRIER.Bilirubin: A bile pigment that is a degradation product of HEME.Bile Pigments: Linear TETRAPYRROLES that give a characteristic color to BILE including: BILIRUBIN; BILIVERDIN; and bilicyanin.Hyperbilirubinemia: A condition characterized by an abnormal increase of BILIRUBIN in the blood, which may result in JAUNDICE. Bilirubin, a breakdown product of HEME, is normally excreted in the BILE or further catabolized before excretion in the urine.Diazonium CompoundsGenotype: The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.Treatment Outcome: Evaluation undertaken to assess the results or consequences of management and procedures used in combating disease in order to determine the efficacy, effectiveness, safety, and practicability of these interventions in individual cases or series.Rats, Gunn: Mutant strain of Rattus norvegicus which is used as a disease model of kernicterus.Occludin: A MARVEL domain protein that plays an important role in the formation and regulation of the TIGHT JUNCTION paracellular permeability barrier.Junctional Adhesion Molecules: A family of membrane glycoproteins localized to TIGHT JUNCTIONS that contain two extracellular Ig-like domains, a single transmembrane segment, and a cytoplasmic tail of variable length.Tight Junctions: Cell-cell junctions that seal adjacent epithelial cells together, preventing the passage of most dissolved molecules from one side of the epithelial sheet to the other. (Alberts et al., Molecular Biology of the Cell, 2nd ed, p22)Zonula Occludens-1 Protein: A 195-kDa zonula occludens protein that is distinguished by the presence of a ZU5 domain at the C-terminal of the molecule.Junctional Adhesion Molecule C: A junctional adhesion molecule subtype that is expressed at high levels in PLACENTA; BRAIN; KIDNEY; and PLATELETS. It serves a variety of functions such as mediating leukocyte-platelet interactions, regulating trans-epithelial migration of POLYMORPHONUCLEAR LEUKOCYTES, and acting as a counter receptor for ALPHAM INTEGRIN.Cell Adhesion Molecules: Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.Cell Polarity: Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.Cell Adhesion: Adherence of cells to surfaces or to other cells.Schwann Cells: Neuroglial cells of the peripheral nervous system which form the insulating myelin sheaths of peripheral axons.Cadherins: Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.Selectins: Transmembrane proteins consisting of a lectin-like domain, an epidermal growth factor-like domain, and a variable number of domains that are homologous to complement regulatory proteins. They are important cell adhesion molecules which help LEUKOCYTES attach to VASCULAR ENDOTHELIUM.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Axons: Nerve fibers that are capable of rapidly conducting impulses away from the neuron cell body.Armenia: An ancient country in western Asia, by the twentieth century divided among the former USSR, Turkey, and Iran. It was attacked at various times from before the 7th century B.C. to 69 B.C. by Assyrians, Medes, Persians, the Greeks under Alexander, and the Romans. It changed hands frequently in wars between Neo-Persian and Roman Empires from the 3d to 7th centuries and later under Arabs, Seljuks, Byzantines, and Mongols. In the 19th century Armenian nationalism arose but suffered during Russo-Turkish hostilities. It became part of the Soviet Republic in 1921, with part remaining under Turkey. (Webster's New Geographical Dictionary, 1988)Theaceae: A plant family of the order THEALES, subclass Dilleniidae, class Magnoliopsida, best known for CAMELLIA SINENSIS, which is the source of Oriental TEA.Quinolinium CompoundsFluorescent Dyes: Agents that emit light after excitation by light. The wave length of the emitted light is usually longer than that of the incident light. Fluorochromes are substances that cause fluorescence in other substances, i.e., dyes used to mark or label other compounds with fluorescent tags.HydrazinesOrganic Chemicals: A broad class of substances containing carbon and its derivatives. Many of these chemicals will frequently contain hydrogen with or without oxygen, nitrogen, sulfur, phosphorus, and other elements. They exist in either carbon chain or carbon ring form.Intensive Care Units, Pediatric: Hospital units providing continuous surveillance and care to acutely ill infants and children. Neonates are excluded since INTENSIVE CARE UNITS, NEONATAL is available.Critical Illness: A disease or state in which death is possible or imminent.Nutritional Support: The administration of nutrients for assimilation and utilization by a patient by means other than normal eating. It does not include FLUID THERAPY which normalizes body fluids to restore WATER-ELECTROLYTE BALANCE.Parenteral Nutrition: The administering of nutrients for assimilation and utilization by a patient who cannot maintain adequate nutrition by enteral feeding alone. Nutrients are administered by a route other than the alimentary canal (e.g., intravenously, subcutaneously).Parenteral Nutrition, Total: The delivery of nutrients for assimilation and utilization by a patient whose sole source of nutrients is via solutions administered intravenously, subcutaneously, or by some other non-alimentary route. The basic components of TPN solutions are protein hydrolysates or free amino acid mixtures, monosaccharides, and electrolytes. Components are selected for their ability to reverse catabolism, promote anabolism, and build structural proteins.Intestinal Mucosa: Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.Enteral Nutrition: Nutritional support given via the alimentary canal or any route connected to the gastrointestinal system (i.e., the enteral route). This includes oral feeding, sip feeding, and tube feeding using nasogastric, gastrostomy, and jejunostomy tubes.Neocortex: The largest portion of the CEREBRAL CORTEX in which the NEURONS are arranged in six layers in the mammalian brain: molecular, external granular, external pyramidal, internal granular, internal pyramidal and multiform layers.Vascular Endothelial Growth Factor A: The original member of the family of endothelial cell growth factors referred to as VASCULAR ENDOTHELIAL GROWTH FACTORS. Vascular endothelial growth factor-A was originally isolated from tumor cells and referred to as "tumor angiogenesis factor" and "vascular permeability factor". Although expressed at high levels in certain tumor-derived cells it is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability it may play a role in stimulating VASODILATION via NITRIC OXIDE-dependent pathways. Alternative splicing of the mRNA for vascular endothelial growth factor A results in several isoforms of the protein being produced.Hypoxia-Inducible Factor 1, alpha Subunit: Hypoxia-inducible factor 1, alpha subunit is a basic helix-loop-helix transcription factor that is regulated by OXYGEN availability and is targeted for degradation by VHL TUMOR SUPPRESSOR PROTEIN.Neovascularization, Pathologic: A pathologic process consisting of the proliferation of blood vessels in abnormal tissues or in abnormal positions.Cell Hypoxia: A condition of decreased oxygen content at the cellular level.Brain: The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.Hypoxia-Inducible Factor 1: A basic helix-loop-helix transcription factor that plays a role in APOPTOSIS. It is composed of two subunits: ARYL HYDROCARBON RECEPTOR NUCLEAR TRANSLOCATOR and HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT.Drug Delivery Systems: Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.Membranes, Artificial: Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.Chemistry, Pharmaceutical: Chemistry dealing with the composition and preparation of agents having PHARMACOLOGIC ACTIONS or diagnostic use.Delayed-Action Preparations: Dosage forms of a drug that act over a period of time by controlled-release processes or technology.Equipment Design: Methods of creating machines and devices.Porosity: Condition of having pores or open spaces. This often refers to bones, bone implants, or bone cements, but can refer to the porous state of any solid substance.Disease Reservoirs: Animate or inanimate sources which normally harbor disease-causing organisms and thus serve as potential sources of disease outbreaks. Reservoirs are distinguished from vectors (DISEASE VECTORS) and carriers, which are agents of disease transmission rather than continuing sources of potential disease outbreaks.

Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions. (1/114)

In multicellular organisms, various compositionally distinct fluid compartments are established by epithelial and endothelial cellular sheets. For these cells to function as barriers, tight junctions (TJs) are considered to create a primary barrier for the diffusion of solutes through the paracellular pathway [1] [2] [3]. In ultrathin sections viewed under electron microscopy, TJs appear as a series of apparent fusions, involving the outer leaflets of plasma membranes of adjacent cells, to form the so-called kissing points of TJs, where the intercellular space is completely obliterated [4]. Claudins are a family of 16 proteins whose members have been identified as major integral membrane proteins localized exclusively at TJs [5] [6] [7] [8]. It remains unclear, however, whether claudins have the cell-adhesion activity that would explain the unusual intercellular adhesion at TJs. Using mouse L-fibroblast transfectants expressing various amounts of claudin-1, -2 or -3, we found that these claudins possess Ca(2+)-independent cell-adhesion activity. Using ultrathin-section electron microscopy, we observed many kissing points of TJs between adjacent transfectants. Furthermore, the cell-adhesion activity of occludin, another integral membrane protein localized at TJs [9] [10] [11], was negligible when compared with that of claudins. Thus, claudins are responsible for TJ-specific obliteration of the intercellular space.  (+info)

Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: Evidence for direct involvement of claudins in tight junction barrier. (2/114)

Claudins, comprising a multigene family, constitute tight junction (TJ) strands. Clostridium perfringens enterotoxin (CPE), a single approximately 35-kD polypeptide, was reported to specifically bind to claudin-3/RVP1 and claudin-4/CPE-R at its COOH-terminal half. We examined the effects of the COOH-terminal half fragment of CPE (C-CPE) on TJs in L transfectants expressing claudin-1 to -4 (C1L to C4L, respectively), and in MDCK I cells expressing claudin-1 and -4. C-CPE bound to claudin-3 and -4 with high affinity, but not to claudin-1 or -2. In the presence of C-CPE, reconstituted TJ strands in C3L cells gradually disintegrated and disappeared from their cell surface. In MDCK I cells incubated with C-CPE, claudin-4 was selectively removed from TJs with its concomitant degradation. At 4 h after incubation with C-CPE, TJ strands were disintegrated, and the number of TJ strands and the complexity of their network were markedly decreased. In good agreement with the time course of these morphological changes, the TJ barrier (TER and paracellular flux) of MDCK I cells was downregulated by C-CPE in a dose-dependent manner. These findings provided evidence for the direct involvement of claudins in the barrier functions of TJs.  (+info)

Manner of interaction of heterogeneous claudin species within and between tight junction strands. (3/114)

In tight junctions (TJs), TJ strands are associated laterally with those of adjacent cells to form paired strands to eliminate the extracellular space. Claudin-1 and -2, integral membrane proteins of TJs, reconstitute paired TJ strands when transfected into L fibroblasts. Claudins comprise a multigene family and more than two distinct claudins are coexpressed in single cells, raising the questions of whether heterogeneous claudins form heteromeric TJ strands and whether claudins interact between each of the paired strands in a heterophilic manner. To answer these questions, we cotransfected two of claudin-1, -2, and -3 into L cells, and detected their coconcentration at cell-cell borders as elaborate networks. Immunoreplica EM confirmed that distinct claudins were coincorporated into individual TJ strands. Next, two L transfectants singly expressing claudin-1, -2, or -3 were cocultured and we found that claudin-3 strands laterally associated with claudin-1 and -2 strands to form paired strands, whereas claudin-1 strands did not interact with claudin-2 strands. We concluded that distinct species of claudins can interact within and between TJ strands, except in some combinations. This mode of assembly of claudins could increase the diversity of the structure and functions of TJ strands.  (+info)

Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane protein. (4/114)

Claudins (claudin-1 to -18) with four transmembrane domains and two extracellular loops constitute tight junction strands. The peptide toxin Clostridium perfringens enterotoxin (CPE) has been shown to bind to claudin-3 and -4, but not to claudin-1 or -2. We constructed claudin-1/claudin-3 chimeric molecules and found that the second extracellular loop of claudin-3 conferred CPE sensitivity on L fibroblasts. Furthermore, overlay analyses revealed that the second extracellular loop of claudin-3 specifically bound to CPE at the K(a) value of 1.0x10(8) M(-1). We concluded that the second extracellular loop is the site through which claudin-3 interacts with CPE on the cell surface.  (+info)

Claudin promotes activation of pro-matrix metalloproteinase-2 mediated by membrane-type matrix metalloproteinases. (5/114)

Genes associated with regulation of membrane-type matrix metalloproteinase-1 (MT1-MMP)-mediated pro-MMP-2 processing were screened in 293T cells by a newly developed expression cloning method. One of the gene products, which promoted processing of pro-MMP-2 by MT1-MMP was claudin-5, a major component of endothelial tight junctions. Expression of claudin-5 not only replaced TIMP-2 in pro-MMP-2 activation by MT1-MMP but also promoted activation of pro-MMP-2 mediated by all MT-MMPs and MT1-MMP mutants lacking the transmembrane domain (DeltaMT1-MMP). A carboxyl-terminal deletion mutant of pro-MMP-2 (proDeltaMMP-2) was processed to an intermediate form by MT1-MMP in 293T cells and was further converted to an activated form by introduction of claudin-5. In contrast to the stimulatory effect of TIMP-2 on pro-MMP-2 activation by MT1-MMP, activation of pro-MMP-2 by DeltaMT1-MMP in the presence of claudin-5 and proDeltaMMP-2 processing by MT1-MMP were both inversely repressed by expression of exogenous TIMP-2. These results suggest that TIMP-2 is not involved in cluadin-5-induced pro-MMP-2 activation by MT-MMPs. Stimulation of MT-MMP-mediated pro-MMP-2 activation was also observed with other claudin family members, claudin-1, claudin-2, and claudin-3. Amino acid substitutions or deletions in ectodomain of claudin-1 abolished stimulatory effect. Direct interaction of claudin-1 with MT1-MMP and MMP-2 was demonstrated by immunoprecipitation analysis. MT1-MMP was co-localized with claudin-1 not only at cell-cell borders, but also at other parts of the cells. TIMP-2 enhanced cell surface localization of MMP-2 mediated by MT1-MMP, and claudin-1 also stimulated it. These results suggest that claudin recruits all MT-MMPs and pro-MMP-2 on the cell surface to achieve elevated focal concentrations and, consequently, enhances activation of pro-MMP-2.  (+info)

Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium. (6/114)

The mRNA for Rvp.1 (rat ventral prostate) increases in abundance before gland involution after androgen deprivation. Rvp.1 is homologous to CPE-R, the high-affinity intestinal epithelial receptor for Clostridium perfringens enterotoxin (CPE), and is sufficient to mediate CPE binding and trigger subsequent toxin-mediated cytolysis. Rvp.1 (claudin-3) and CPE-R (claudin-4) are members of a larger family of transmembrane tissue-specific claudin proteins that are essential components of intercellular tight junction structures regulating paracellular ion flux. However, claudin-3 and claudin-4 are the only family members capable of mediating CPE binding and cytolysis. The present study was designed to study the expression of claudin-3 and claudin-4 in human prostate tissue as potential targets for CPE toxin-mediated therapy for prostate cancer. On human multiple-tissue Northern blot analysis, mRNAs for both claudin-3 and claudin-4 were expressed at high levels in prostate tissue. In normal prostate tissue, expression of claudin-3 was localized exclusively within acinar epithelial cells by in situ mRNA hybridization. Compared with expression within prostate epithelial cells in surrounding normal glandular tissue, expression of claudin-3 mRNA remained high in the epithelium of prostate adenocarcinoma (10 of 10) and prostatic intraepithelial neoplasia (five of five). Prostate adenocarcinoma cells metastatic to bone were obtained from a patient with disease progression during antiandrogen therapy. These metastatic cells were prostate-specific antigen-positive by immunohistochemical staining and also expressed functional CPE receptors as measured by sensitivity to CPE-induced cell lysis. The persistent high level of claudin-3 expression in prostate adenocarcinoma and functional cytotoxicity of CPE in metastatic androgen-independent prostate adenocarcinoma suggests a new potential therapeutic strategy for prostate cancer.  (+info)

The renal segmental distribution of claudins changes with development. (7/114)

BACKGROUND: Permeability properties of mammalian nephron are tuned during postnatal maturation. The transepithelial electrical resistance (TER) and complexity of tight junctions (TJs) vary along the different tubular segments, suggesting that the molecules constituting this structure change. We studied the differential expression of occludin and several claudins in isolated renal tubules from newborn and adult rabbits. METHODS: Isolated renal tubules from newborn and adult rabbits were processed for occludin, claudin-1 and claudin-2 immunofluorescence, and Western blot detection of claudin-1 and -2. Claudin-5 was detected in whole kidney frozen sections. RT-PCR from isolated tubules was performed for claudins-1 to -8. RESULTS: Immunofluorescence revealed that occludin, claudin-1 and -2 were present at the cell boundaries at the neonatal stage of development. Claudin-1 was detected in the tighter segments of the nephron (distal and collecting duct), while claudin-2 was found in the leaky portions (proximal). Claudin 5 was found in the kidney vasculature. PCR amplification revealed the presence of claudins-1 to -4 in tubules of newborns. In adults, claudins-1, -2 and -4 were present in proximal, Henle's loop and collecting segments; claudin-3 was in proximal and collecting tubules, while claudins-5 and -6 were absent from all tubular portions. Claudin-7 was restricted to proximal tubules, while claudin-8 was present in proximal and Henle's segments. CONCLUSIONS: The pattern of occludin distribution is present from the neonatal age. Claudins-7 and -8 are up-regulated after birth. Each tubular segment expresses a peculiar set of claudins that might be responsible for the permeability properties of their TJs.  (+info)

Claudin localization in cilia of the retinal pigment epithelium. (8/114)

Using immunocytochemistry and confocal microscopy we demonstrate that claudin-immunoreactivity is a novel marker for retinal pigment epithelial cilia. Claudin-immunoreactivity obtained by polyclonal anti-claudin 1 antibody, which could crossreact with claudin 3, was colocalized with acetylated tubulin-immunoreactivity in cultured human retinal pigment epithelial cells. Claudin-immunoreactivity associated with the retinal pigment epithelium (RPE) cilia was more intense than was claudin-immunoreactivity in the junctional complex. Approximately two-thirds of the RPE cells in the rat contain cilia that are immunoreactive with acetylated tubulin on postnatal day 1, and a significant portion of these cilia label with the anti-claudin 1 antibody. Cilia decrease in frequency over subsequent postnatal days, and are absent by postnatal day 30. As RPE cilia decrease in number during postnatal rat development, claudin-immunoreactivity is lost earlier than acetylated tubulin, suggesting that the loss of claudin may initiate RPE cilium degeneration. Claudin-immunoreactivity was not evident in cilia of photoreceptor cells, epithelia of nasal mucosa, small intestine, or colon, suggesting that claudin may be a unique molecule in RPE cilia. These data suggest that cilia of the RPE, unlike cilia on other cell types, contain claudin, and that this molecule may play an important and specific role in the function and/or maintenance of RPE cilia.  (+info)

*CLDN3

Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. It is a member of the claudin ... The protein encoded by this intron-less gene, a member of the claudin family, is an integral membrane protein and a component ... Miyamori H, Takino T, Kobayashi Y, Tokai H, Itoh Y, Seiki M, Sato H (2001). "Claudin promotes activation of pro-matrix ... Tsukita S, Furuse M (2002). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...

*Clostridium enterotoxin

This allows the human claudin-3,4,6,7,8 and 14 to bind but not 1,2,5, and 10. The way the protein work is it destroys the cell ... Van Itallie CM, Betts L, Smedley JG, McClane BA, Anderson JM (January 2008). "Structure of the claudin-binding domain of ... This mechanism is mediated by host claudin-3 and claudin-4 receptors, situated at the tight junctions. Clostridium enterotoxin ... "Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium". Cancer ...

*Janet Sawicki

Huang, YH; Bao, Y; Peng, W; Goldberg, M; Love, K; Bumcrot, DA; Cole, G; Langer, R; Anderson, DG; Sawicki, JA (2009). "Claudin-3 ... doi:10.1016/0012-1606(77)90106-3. PMID 409635. Yasbin, R; Sawicki, J; MacIntyre, RJ (1978). "A developmental study of acid ...

*Pore-forming toxin

"Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane ... 476 (3): 258-61. doi:10.1016/S0014-5793(00)01744-0. PMID 10913624. Barth H, Aktories K, Popoff MR, Stiles BG (September 2004 ... 68 (3): 373-402, table of contents. doi:10.1128/MMBR.68.3.373-402.2004. PMC 515256 . PMID 15353562. Tilley SJ, Orlova EV, ... ISBN 0-8153-3218-1. F. Gisou van der Goot, Pore-forming toxins, Springer, 2001, ISBN 3-540-41386-3 A deadly toxin with a ...

*Cingulin-like 1

1778 (3): 601-13. doi:10.1016/j.bbamem.2007.09.032. PMID 18339298. Pulimeno P, Paschoud S, Citi S (May 13, 2011). "A role for ... However, it leads to increased levels of the mRNA encoding the tight junction proteins ZO-3 and claudin-2, as well as increased ... 86 (3-5): 219-24. doi:10.1016/S0960-0760(03)00359-5. PMID 14623514. Demura M, Bulun SE (February 2008). "CpG dinucleotide ... levels of the ZO-3 protein, whereas when combined with the depletion of cingulin, it causes a decrease in the expression of ...

*Cingulin

... claudin-2, claudin-6, claudin-7 and occludin) and transcription factors (including GATA4). Changes in the expression of claudin ... Paschoud S, Bongiovanni M, Pache JC, Citi S (September 2007). "Claudin-1 and claudin-5 expression patterns differentiate lung ... Guillemot L, Citi S (August 2006). "Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase ... Together with paracingulin, cingulin also was reported to regulate claudin-2 expression through RhoA-dependent and independent ...

*MMP3

The WT mice were shown to have lower claudin-5 and occludin levels than the KO mice after TBI. Claudin and occludin are ... Furuse M, Fujita K, Hiiragi T, Fujimoto K, Tsukita S (Jun 1998). "Claudin-1 and -2: novel integral membrane proteins localizing ... Both the WT and KO tissues showed a drop in claudin-5, occludin, and laminin-α1 (a basal lamina protein), suggesting that MMP-3 ... "Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice". The Journal of Cell Biology. 161 (3): 653-60 ...

*Chromosome 3 (human)

Claudin domain containing 1 CPN2: Carboxypeptidase N subunit 2 CPOX: coproporphyrinogen oxidase (coproporphyria, ... Chromosome 3 is one of the 23 pairs of chromosomes in humans. People normally have two copies of this chromosome. Chromosome 3 ... "Search results - 3[CHR] AND "Homo sapiens"[Organism] AND ("has ccds"[Properties] AND alive[prop]) - Gene". NCBI. CCDS Release ... "Search results - 3[CHR] AND "Homo sapiens"[Organism] AND ("genetype protein coding"[Properties] AND alive[prop]) - Gene". NCBI ...

*Rubielos de la Cérida impact structure

Claudin, F., Ernstson, K., Rampino, M.R., and Anguita, F. 2001. Striae, polish, imprints, rotated fractures, and related ... Ernstson, K., Claudin, F., Schüssler, U., Anguita, F. and Ernstson, T. 2001. Impact melt rocks, shock metamorphism, and ... Missing or empty ,title= (help) Ernstson, K., Claudin, F., Schüssler, U. & Hradil, K. (2002): The mid-Tertiary Azuara and ... 1], Ernstson, K., Schüssler, U., Claudin, F., and Ernstson, T. (2003). An impact crater chain in northern Spain. Meteorite, 9, ...

*CLDN11

Claudin-11 is a protein that in humans is encoded by the CLDN11 gene. It belongs to the group of claudins. The protein encoded ... 2001). "Osp/Claudin-11 Forms a Complex with a Novel Member of the Tetraspanin Super Family and β1 Integrin and Regulates ... "Entrez Gene: CLDN11 claudin 11 (oligodendrocyte transmembrane protein)". Human CLDN11 genome location and CLDN11 gene details ... 2000). "CNS myelin and sertoli cell tight junction strands are absent in Osp/claudin-11 null mice". Cell. 99 (6): 649-59. doi: ...

*CLDN7

Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. It belongs to the group of claudins. Claudins, such as ... "Entrez Gene: CLDN7 claudin 7". Human CLDN7 genome location and CLDN7 gene details page in the UCSC Genome Browser. Kniesel U, ... 2005). "Claudin-1 is a strong prognostic indicator in stage II colonic cancer: a tissue microarray study". Mod. Pathol. 18 (4 ... 2003). "Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and ...

*CLDN19

Claudin-19 is a protein that in humans is encoded by the CLDN19 gene. It belongs to the group of claudins. Claudin-19 has been ... CLDN19 claudin 19". Naeem, M.; Hussain, S.; Akhtar, N. (2011). "Mutation in the Tight-Junction Gene Claudin 19 (CLDN19) and ... 2006). "Kidney claudin-19: localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease". ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...

*CLDN1

"Entrez Gene: CLDN1 claudin 1". Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG (Nov 2003). "Role of claudin ... Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. It belongs to the group of claudins. Tight junctions ... The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight ... Miyamori H, Takino T, Kobayashi Y, Tokai H, Itoh Y, Seiki M, Sato H (2001). "Claudin promotes activation of pro-matrix ...

*Magnesium transporter

The gene Claudin-16 was cloned by Simon et al. (1999), but only after a series of reports described the Mg2+ flux itself with ... In particular, Claudin-16 allows the selective reuptake of Mg2+ in the human kidney. Some patients with mutations in the CLDN19 ... Naeem M, Hussain S, Akhtar N (2011). "Mutation in the tight-junction gene claudin 19 (CLDN19) and familial hypomagnesemia, ... "Mutations in the tight-junction gene claudin 19 (CLDN19) are associated with renal magnesium wasting, renal failure, and severe ...

*CLDN5

Claudin-5 is a protein that in humans is encoded by the CLDN5 gene. It belongs to the group of claudins. This gene encodes a ... "Entrez Gene: CLDN5 claudin 5 (transmembrane protein deleted in velocardiofacial syndrome)". Coyne CB, Gambling TM, Boucher RC, ... Kojima S, Rahner C, Peng S, Rizzolo LJ (2002). "Claudin 5 is transiently expressed during the development of the retinal ... Tsukita S, Furuse M (2002). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...

*CLDN4

Claudin 4, also known as CLDN4, is a protein which in humans is encoded by the CLDN4 gene. It belongs to the group of claudins ... This gene encodes an integral membrane protein, which belongs to the claudin family. The protein is a component of tight ... 2003). "Claudin-4 expression decreases invasiveness and metastatic potential of pancreatic cancer". Cancer Res. 63 (19): 6265- ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...

*List of extraterrestrial dune fields

Jia, P.; B. Andreotti; P. Claudin (March 2017). "Giant ripples on comet 67P/Churyumov-Gerasimenko sculpted by sunset thermal ... ISBN 978-3-540-89724-8. "Titan's Seas Are Sand". Archived from the original on 29 September 2006. Retrieved 21 November 2006. ...

*CLDND1

Claudin domain-containing protein 1 is a protein that in humans is encoded by the CLDND1 gene. GRCh38: Ensembl release 89: ... CLDND1 claudin domain containing 1". Human CLDND1 genome location and CLDND1 gene details page in the UCSC Genome Browser. Liu ... 56 (3): 307-17. doi:10.1016/j.lungcan.2007.01.016. PMID 17316888. Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence ... doi:10.1016/S0378-1119(02)00507-3. PMID 12036590. Zhang QH, Ye M, Wu XY, et al. (2001). "Cloning and Functional Analysis of ...

*Manuel Azcárate

ISBN 978-84-7222-878-8. Azcárate, Manuel; Fernando Claudin (1979). Marc Abeles; Charles-Albert Ryng, eds. L'Europe de ... ISBN 978-84-336-0193-3. Azcárate, Manuel (1982). preface. Le Mouvement communiste à la croisée des chemins. By Schaff, Adam (in ... ISBN 2-7071-1102-3. Azcárate, Manuel (1980). Interrogantes ante la izquierda (in Spanish). Marc Abeles, Charles-Albert Ryng, ...

*CLDN9

2007). "Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus". J. Virol. 81 (22): 12465-71. doi: ... Claudin-9 is a protein that in humans is encoded by the CLDN9 gene. It belongs to the group of claudins. This gene is involved ... "Entrez Gene: CLDN9 claudin 9". Gene discovery reveals a critical protein's function in hearing Human CLDN9 genome location and ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...

*TSPAN8

... claudin-7, CD44 variant isoforms, and tetraspanins promotes colorectal cancer progression". Molecular Cancer Research. 5 (6): ... 38 (3): 577-88. doi:10.1053/jhep.2003.50376. PMID 12939584. Kuhn S, Koch M, Nübel T, Ladwein M, Antolovic D, Klingbeil P, ... doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Serru V, Le Naour F, Billard M, Azorsa DO, Lanza F, Boucheix C, Rubinstein E ( ...

*Dichotomyctere ocellatus

Duffy, N. M.; Bui, P.; Bagherie-lachidan, M.; Kelly, S. P. (2011). "Epithelial remodeling and claudin mRNA abundance in the ... doi:10.1007/s00360-010-0517-3. The Puffer Forum, http://www.thepufferforum.com/forum/ug.php/v/PufferPedia/Brackish/T_ ...

*CLDN12

Claudin-12 is a protein that in humans is encoded by the CLDN12 gene. It belongs to the group of claudins. GRCh38: Ensembl ... 2001). "claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor, encodes lung- and ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... 2004). "Distribution of the tight junction proteins ZO-1, occludin, and claudin-4, -8, and -12 in bladder epithelium". Am. J. ...

*1562 in France

Claudin de Sermisy, composer (born c.1490) 31 October - Augustin Marlorat. Protestant reformer (born 1506) 19 December - ... 1915). "Biron, Charles de Gontaut". Salmonsens Konversationsleksikon (in Danish). 3 (2 ed.). Copenhagen: J.H. Schultz ...

*TMEM47

This gene encodes a member of the PMP22/EMP/claudin protein family. The encoded protein is localized to the ER and the plasma ... 1 (3): 287-92. doi:10.1093/embo-reports/kvd058. PMC 1083732 . PMID 11256614. Christophe-Hobertus C, Kooy F, Gecz J, Abramowicz ... 11 (3): 422-35. doi:10.1101/gr.GR1547R. PMC 311072 . PMID 11230166. Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, ... 2: 3. doi:10.1186/1471-2164-2-3. PMC 35279 . PMID 11472633. "Entrez Gene: TMEM47 transmembrane protein 47". Hartley JL, Temple ...

*Intestinal epithelium

These complexes, formed primarily of members of the claudin and the occludin families, consist of about 35 different proteins, ... 124 (1): 3-22. doi:10.1016/j.jaci.2009.05.038. ISSN 0091-6749. PMC 4266989 . PMID 19560575. Bennett, M. V.; Barrio, L. C.; ... 6 (3): 305-320. doi:10.1016/0896-6273(91)90241-q. ISSN 0896-6273. PMID 1848077. Nekrasova, Oxana; Green, Kathleen J. (2013-11- ...
Claudins are major integral membrane proteins of tight junctions. Altered expression of several claudin proteins, in particular claudin-1, -3, -4 and -7, has been linked to the development of various cancers. Although their dysregulation in cancer suggests that claudins play a role in tumorigenesis, the exact underlying mechanism remains unclear. The involvement of claudins in tumor progression was suggested by their important role in the migration, invasion and metastasis of cancer cells in a tissue-dependent manner. Recent studies have shown that they play a role in epithelial to mesenchymal transition (EMT), the formation of cancer stem cells or tumor-initiating cells (CSCs/TICs), and chemoresistance, suggesting that claudins are promising targets for the treatment of chemoresistant and recurrent tumors. A recently identified claudin-low breast cancer subtype that is characterized by the enrichment of EMT and stem cell-like features is significantly associated with disease recurrence, underscoring
View mouse Cldn15 Chr5:136966616-136975858 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
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The entire human body and its many compartments are shielded from their external environments by the barrier function of epithelial cell sheets. The paracellular barrier function of tight junctions (TJs) is critical for maintaining homeostasis in any multicellular organism, especially in the skin and internal organs and at the blood-brain barrier. One of the major components of TJs is a family of adhesive membrane proteins known as claudins. Several members of the claudin family are receptors for the bacterial toxin Clostridium perfringens enterotoxin. This toxin often causes food-borne illness both in humans and animals. Saitoh et al. crystallized a complex between the toxin and a claudin that reveals just how the toxin damages epithelial barriers (see the Perspective by Artursson and Knight).. Science, this issue p. 775; see also p. 716 ...
In this study, phosphate-rich supernatant at the end of anaerobic phase was extracted by a certain side-stream ratio for chemical precipitation to investigate the optimal conditions for phosphorus recovery. The effect of side-stream reaction on the performance of the mainstream Enhanced Biological Phosphorus Removal (EBPR) system was also explored. The experiment was carried out in a sequencing batch reactor (SBR) operated in an alternating anaerobic/aerobic mode with dissolved oxygen controlled at 1.0 mg · L−1. The results showed that the optimum magnesium source,temperature, stirring speed and reaction equilibrium time for side-stream phosphorus recovery were: MgCl2 · 6H2O, 25 °C, 150 rpm and 20 min, respectively. It was also observed that the average phosphorus removal efficiency of the mainstream system maintained as high as 90.7% during the side-stream extraction period despite insufficient time for phosphate uptake under limited dissolved oxygen condition and phosphate deprivation of ...
The ability to invade host tissues and metastasize is the major cause of cancer-related death. During tumor invasion, metastasizing cells disrupt normal cell-matrix adhesion and acquire an invasive phenotype. Claudins are adhesion proteins localized at tight junctions (TJs). Claudin-7 is a unique TJ membrane protein in that it has a stronger basolateral membrane distribution than that of apical TJs in epithelial cells. To study the basolateral function of claudin-7, claudin-7 gene silencing experiments were carried out in a lung cancer cell line using the lentivirus shRNA approach. We found that claudin-7 knockdown (KD) cells showed disrupted cell-matrix interactions. Consequently, when claudin-7 KD cells were plated on the uncoated glass surface, they were unable to attach to the glass and died the day after plating. In contrast, control cells adhered well and grew normally. Using immunofluorescent microscopy and biochemistry methods, we found that claudin-7 co-localized and ...
A side-stream respiration monitoring sensor includes a body having a first end, a second end, and a detecting section disposed between the respective ends. The detecting section includes a first port and a second port positioned on generally opposite sides of a restricting member. The restricting member extends into a flow path formed through the body such that a pressure differential is generated between the first port and the second port. A sampling port is positioned downstream relative to a patient from the first and the second port and configured to acquire a respiration sample. The sensor is constructed to monitor respiration performance of premature infants
GGRNA , 2020-04-07 23:23:15 , RefSeq release 60 (20130726)] RefSeq ID Version Symbol GeneID Definition NM_001101389 NM_001101389.1 CLDN25 644672 Homo sapiens claudin 25 (CLDN25), mRNA. NM_001111319 NM_001111319.1 CLDN22 53842 Homo sapiens claudin 22 (CLDN22), mRNA. NM_020982 NM_020982.3 CLDN9 9080 Homo sapiens claudin 9 (CLDN9), mRNA. NM_001001346 NM_001001346.3 CLDN20 49861 Homo sapiens claudin 20 (CLDN20), mRNA. NM_001306 NM_001306.3 CLDN3 1365 Homo sapiens claudin 3 (CLDN3), mRNA. NM_012131 NM_012131.2 CLDN17 26285 Homo sapiens claudin 17 (CLDN17), mRNA. NM_016369 NM_016369.3 CLDN18 51208 Homo sapiens claudin 18 (CLDN18), transcript variant 1, mRNA. NM_001185056 NM_001185056.1 CLDN11 5010 Homo sapiens claudin 11 (CLDN11), transcript variant 2, mRNA. NM_005602 NM_005602.5 CLDN11 5010 Homo sapiens claudin 11 (CLDN11), transcript variant 1, mRNA. NM_182848 NM_182848.3 CLDN10 9071 Homo sapiens claudin 10 (CLDN10), transcript variant a, mRNA. NM_006984 NM_006984.4 CLDN10 9071 Homo sapiens claudin ...
Claudin-4 (Clostridium perfringens enterotoxin receptor) is a tight junction protein encoded by the gene CLDN4. Expression of Claudin-4 has been associated with either poor prognosis or a more favorable diagnosis, depending on the type of cancer. Claudin-4 has been shown to distinguish adenocarcinoma from malignant mesothelioma with 99% specificity in malignant effusions (1). Claudin-4 overexpression was able to independently predict survival in a breast cancer multivariate analysis as it was associated with poor prognosis, high tumor grade and Her2 expression and was inversely correlated with estrogen receptor staining (2). In luminal breast cancer, the increase of Claudin-4 protein was correlated with the increase of tumor grade and with Ki-67, and thus demonstrated an overall shorter life survival (3). Basal-like tumors also demonstrated overexpression of Claudin-4 (4). Counter to the above breast cancer subtypes, the presence of Claudin-4 in triple negative breast cancer was a biomarker that
As mentioned in our introduction, the availability of CPE-directed therapeutics could be helpful for ameliorating several CPE-associated medical conditions. A previous study had suggested that the drug mepacrine might be a candidate CPE therapeutic because the presence of this drug interferes with CPE-induced electrophysiologic activity in artificial lipid bilayers (24). However, that study did not distinguish whether mepacrine inactivates the CPE protein or instead interferes with some step in CPE action, i.e., whether this drug affects CPE binding, CPE pore formation, or CPE pore activity. Furthermore, it was specifically important to determine whether mepacrine is not only protective against CPE electrophysiologic activity in artificial membranes but also inhibits CPE-induced cytotoxicity in mammalian cells, where receptors are present and complex phenomena like membrane vesicle release occur (30).. Therefore, a first major contribution of the current study entailed demonstrating that ...
Open peer review is a system where authors know who the reviewers are, and the reviewers know who the authors are. If the manuscript is accepted, the named reviewer reports are published alongside the article. Pre-publication versions of the article and author comments to reviewers are available by contacting [email protected] All previous versions of the manuscript and all author responses to the reviewers are also available.. You can find further information about the peer review system here.. ...
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The expression of claudin-11 in benign and malignant bladder tissue and the effect of forced expression of claudin-11 on tight junction function and invasiveness of bladder cancer cells were studied. Claudin-11 expression was tested in bladder cancer cell lines (T24/83, RT 112/84 and EJ138) using reverse transcription-polymerase chain reaction (RT-PCR) and in benign and malignant bladder tissue by quantitative RT-PCR and immunohistochemistry. T24/83 cells were transfected with the pcDNA.1/NT-GFP-TOPO vector containing full-length human claudin-11 sequence. Stable-transfected cells overexpressing claudin-11 (T24Cl-11Ex), wild-type cells (T24WT) and the empty plasmid control clone (T24GFP) were compared using transurothelial resistance (TUR), in vitro adhesion, invasion and growth assays. Claudin-11 was strongly expressed in the non-invasive RT112/84 cell line compared to the invasive T24/83 and EJ138 TCC cell lines. Benign bladder tissue demonstrated equal expression of claudin-11 mRNA as ...
The protein encoded by this intronless gene belongs to the claudin family. Claudins are integral membrane proteins that are components of the epithelial cell tight junctions, which regulate movement of solutes and ions through the paracellular space. This protein is a high-affinity receptor for Clostridium perfringens enterotoxin (CPE) and may play a role in internal organ development and function during pre- and postnatal life. This gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. [provided by RefSeq, Sep 2013 ...
The tight junction protein claudin-1 (CLDN1) has been shown to be essential for hepatitis C virus (HCV) entry-the first step of viral infection. Due to the lack of neutralizing anti-CLDN1 antibodies, the role of CLDN1 in the viral entry process is poorly understood. In this study, we produced antibodies directed against the human CLDN1 extracellular loops by genetic immunization and used these antibodies to investigate the mechanistic role of CLDN1 for HCV entry in an infectious HCV cell culture system and human hepatocytes. Antibodies specific for cell surface-expressed CLDN1 specifically inhibit HCV infection in a dose-dependent manner. Antibodies specific for CLDN1, scavenger receptor B1, and CD81 show an additive neutralizing capacity compared with either agent used alone. Kinetic studies with anti-CLDN1 and anti-CD81 antibodies demonstrate that HCV interactions with both entry factors occur at a similar time in the internalization process. Anti-CLDN1 antibodies inhibit the binding of ...
Claudin-1 is an integral membrane protein component of tight junctions. The Snail family of transcription factors are repressors that play a central role in the epithelial-mesenchymal transition, a process that occurs during cancer progression. Snail and Slug members are direct repressors of E-cadherin and act by binding to the specific E-boxes of its proximal promoter. In the present study, we demonstrate that overexpression of Slug or Snail causes a decrease in transepithelial electrical resistance. Overexpression of Slug and Snail in MDCK (Madin-Darby canine kidney) cells down-regulated Claudin-1 at protein and mRNA levels. In addition, Snail and Slug are able to effectively repress human Claudin-1-driven reporter gene constructs containing the wild-type promoter sequence, but not those with mutations in two proximal E-box elements. We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin-1 promoter. Moreover, an inverse correlation in ...
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudins are small (20-27 kilodalton (kDa)) transmembrane proteins which are found in many organisms, ranging from nematodes to human beings, and are very similar in their structure, although this conservation is not observed on the genetic level. Claudins span the cellular membrane 4 times, with the N-terminal end and the C-terminal end both located in the cytoplasm, and two extracellular loops which show the highest degree of conservation. The first extracellular loop consists on average of 53 amino acids and the second one, being slightly smaller, of 24 amino acids. The N-terminal end is usually very short (4-10 amino acids), the C-terminal end varies in length ...
This study examines the expression of Mab CC188 binding epitope in ovarian cancer stem cells and their differentiated progeny in vitro and explores the feasibility of imaging ovarian xenograft tumors with Mab CC188 labeled with NIR dye using an IRDye 800CW Protein Labeling Kit from LI-COR Biosciences.
Novogen Ltd. surged as much as 263% to $7.60 in regular trading on Tuesday after the company announced that its lead experimental drug, CS-6, has proven to be highly active against ovarian cancer stem cells in laboratory studies.
The C\terminal fragment of enterotoxin (C\CPE) modulates the tight junction protein claudin and disturbs the tight junctional barrier. epithelial cells (HPDEs) had been treated with C\CPE 194 and C\CPE meters19. In well\differentiated cells of the pancreatic malignancy cell collection HPAC, C\CPE 194 and C\CPE meters19 interrupted both the hurdle and fencing features without adjustments in manifestation of claudin\1 and \4, collectively with an boost of MAPK phosphorylation. C\CPE 194, but not really C\CPE meters19, improved the cytotoxicity of the anticancer brokers gemcitabine and H\1. In differentiated pancreatic malignancy cell collection PANC\1 badly, C\CPE 194, but not really C\CPE meters19, reduced claudin\4 phrase and improved MAPK activity and the cytotoxicity of the anticancer agencies. In regular HPDEs, C\CPE 194 and C\CPE meters19 reduced claudin\4 phrase and improved the MAPK activity, whereas they do not really influence the cytotoxicity of the anticancer agencies. Our results ...
For every experimental group, brains from at minimum 3 distinct litter had been analyzed and when compared to the in accordance NaCl handle group. qPCR approach improvement exposed that only samples must be when compared to every other which have gone through experimental treatment, mind isolation, storage, purification and evaluation preparing steps with each other. Therefore, for every DEX-treatment the according NaCl handle group was carried out at the exact same time. In addition, owing to the large complete variety of samples, but limited sample variety which could be purified at the same time, only samples from mice at the same age and identical variety of antenatal injections ended up compared to every other by using a two-tailed Student`s t-take a look at. Data are offered as the signifies ± SEM. The major tight junction molecule and mind endothelial mobile marker claudin-five was investigated originally. Triple maternal DEX remedy drastically decreased claudin-5 mRNA expression to .54 ...
A combined imaging and phototherapy technique could help guide surgeons removing chemotherapy-resistant tumors and kill any cancer cells the surgeons
Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. Chromatin immunoprecipitation (ChIP) and luciferase reporter assay showed that SCF/c-kit
DESCRIPTION (provided by applicant): Disruption of the cell-cell junction with concomitant changes in the expression of junctional proteins is a hallmark of cancer metastasis and invasion. Role of adherent junction proteins have been studied extensively in cancer, however the role of tight junction proteins is less understood. Claudins are the recently identified tetraspanins, which are integral to the structure and function of tight junctions (TJs). Recent studies have shown changes in expression/cellular localization for claudins during tumorigenesis, however a cause and effect relationship has not been established. Here, we report a highly increased expression for claudin-1 in human primary colon carcinoma and metastatic tissues and cell lines derived from similar sources with relatively frequent nuclear localization. Furthermore, using genetic manipulations of claudin-1 expression in colon cancer cell lines, we demonstrate a role for claudin-1 in the regulation of epithelial to mesenchymal ...
Objective Helicobacter pylori strains that express the oncoprotein CagA augment risk for gastric cancer. However, the precise mechanisms through which cag+ strains heighten cancer risk have not been fully delineated and model systems that recapitulate the gastric niche are critical for understanding pathogenesis. Gastroids are three-dimensional organ-like structures that provide unique opportunities to study host-H. pylori interactions in a preclinical model. We used gastroids to inform and direct in vitro studies to define mechanisms through which H. pylori modulates expression of the cancer-associated tight junction protein claudin-7.. ...
Epithelial barrier dysfunction is a significant factor in many allergic diseases, including eosinophilic esophagitis (EoE). Infiltrating leukocytes and tissue adaptations increase metabolic demands and decrease oxygen availability at barrier surfaces. Understanding of how these processes impact barrier is limited, particularly in allergy. Here, we identified a regulatory axis whereby the oxygen-sensing transcription factor HIF-1α orchestrated epithelial barrier integrity, selectively controlling tight junction CLDN1 (claudin-1). Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial barrier function, as documented in 3D organotypic epithelial cultures. L2-IL5OXA mice with EoE-relevant allergic inflammation displayed localized eosinophil oxygen metabolism, tissue hypoxia, and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of esophageal epithelial-targeted stabilized ...
The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.
immune Uncategorized PA-824, Rabbit Polyclonal to C-RAF (phospho-Thr269). The blood-epididymis barrier (BEB) is formed by epithelial tight junctions mediating selective permeability from the PA-824 epididymal epithelium. the paracellular permeability had been examined by two strategies TER and FITC-Dextran-based tracer diffusion assays. Both assays soon add up to related outcomes indicating a time-dependent disruption from the BEB differentially for the three TGF? isoforms (TGF?3>TGF?1>TGF?2) inside a TGF?-recetor-1 kinase- and Smad-dependent way. The small junction proteins claudin-1 was discovered to be decreased by the procedure with TGF?s whereas occludin had not PA-824 been affected. Epididymal epithelial cells are mainly attentive to TGF?s PA-824 through the basolateral side recommending that TGF? may impact for the epididymal epithelium through the stroma cell tradition versions the knockdown of 1 of the claudins (1 -3 -4 or -7) led to dramatically reduced transepithelial electrical level ...
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Sigma-Aldrich offers abstracts and full-text articles by [S K Tiwari-Woodruff, A G Buznikov, T Q Vu, P E Micevych, K Chen, H I Kornblum, J M Bronstein].
Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans cells in the epidermis. Our objective was to identify immunoreactive CLDN1 in human epidermal Langerhans cells and to examine the pattern of epidermal Langerhans cells in genetic human CLDN1 deficiency [neonatal ichthyosis, sclerosing cholangitis (NISCH) syndrome]. Epidermal cells from healthy human skin labelled with CLDN1-specific antibodies were analysed by confocal laser immunofluorescence microscopy and flow cytometry. Skin biopsy sections of two patients with NISCH syndrome were stained with an antibody to CD1a expressed on epidermal Langerhans cells. Epidermal Langerhans cells and a subpopulation of keratinocytes from healthy skin were positive for CLDN1. The gross number and distribution of epidermal Langerhans cells of two patients with molecularly confirmed NISCH syndrome, however, was not grossly altered. Therefore, CLDN1 is unlikely to play a critical role in ...
Am J Reprod Immunol. 2011 Apr;65(4):438-47. doi: 10.1111/j.1600-0897.2010.00914.x. Epub 2010 Sep 6. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
From NCBI Gene:. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in ...
This gene encodes an integral membrane protein, which belongs to the claudin family. The protein is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. This gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems ...
Nestlé has unveiled a new chocolate recipe that replaces refined sugar with cocoa pulp as it begins to explore the possibilities of side-stream utilisation to reduce food waste.
Cytoplasmic expression of claudin-1 in metastatic melanoma cells correlates to increased migration, and increased secretion of MMP-2 in a PKC dependent manner, whereas claudin-1 nuclear expressi...
Complete information for CLDN18 gene (Protein Coding), Claudin 18, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
Tight junctions between epithelial and endothelial cells form selective barriers and paracellular channels and regulate paracellular transport of solutes, immune cells, and drugs. More specifically, tight junctions consist of proteins that laterally interconnect neighboring cells of epithelia and endothelia. Certain proteins seal the tight junction, so that a nearly impermeable barrier develops, whereas others form channels that allow for permeation between the cells. Recent investigations have focused on tight junction proteins, belonging to the claudin family (claudins-1 to -27 in humans) and the newly defined group of TAMP (three proteins: occludin, Marvel-D2, and tricellulin). Barriers and Channels Formed by Tight Junction Proteins I showcases work in this area clustered around three major themes: the molecular properties of tight junctions, for example, the role of the claudin family of proteins and the formation of ion and charge-selective channels; the regulation of tight junction
Crohns disease; infective colitis is often a cause of one episode of colitis which is mislabelled as ulcerative colitis e.g. transverse colon the portion Causes and symptoms. Biocare Probiotics 30 Billion Which Uk Are Best benefits of Digestive Enzymes for Cats. John McDougall in his book The McDougall Plan summarizes the multiple benefits of fiber (some info Ive taken from other sources too): Fiber has no calories since Probiotic dosages are listed Introduction Giardia Clostridium perfringens enterotoxin and Cryptosporidium are important causes of diarrhea in dogs and cats. Find and study online flashcards from Pathophysiology 3400. Colorectal cancer commonly known as colon cancer or bowel cancer is a melanoma from uncontrolled cell growth in the Probiotics can also play Biocare Probiotics 30 Billion Which Uk Are Best a role in maintaining oral health (bad eath). I know there have been some posts dealing with probiotics and many have questions about wh Stage IIB: Cancer has C colon ...
TABLE-US-00004 TABLE 4 Cell and biological adhesion Fold Symbol Gene name Gene assignment change CLDN7 claudin 7 Involved in the formation of 2.74 tight junctions between epithelial cells PCDHB5 Protocadherin Member of the 3.32 beta-5 protocadherin beta gene cluster CLDN3 Claudin 3 Member of the claudin 3.93 family, is an integral membrane protein and a component of tight junction strands. CNTN6 contactin 6 Contactins mediate cell 3.09 surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. PKHD1 polycystic kidney Localized predominantly at 3.38 and hepatic the disease 1 apical domain of polarized epithelial cells, suggesting it may be involved in the tubulogenesis and/or maintenance of duct-lumen architecture. PCDHB2 protocadherin The extracellular domains 2.94 beta 2 interact in a homophilic manner to specify differential cell-cell connections. CDH1 E-cadherin cell adhesion molecule 3.27 (epithelial) CX3CL1 hemokine ...
Claudin 7 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human,mouse samples. Cat.No. 10118-1-AP.
Blood-brain barrier (BBB) leakage plays a key role in cerebral ischemia-reperfusion injury. It is quite necessary to further explore the characteristic and mechanism of BBB leakage during stroke. We induced a focal cerebral ischemia model by transient middle cerebral artery occlusion in male rats for defining the time course of BBB permeability within 120 h following reperfusion and evaluate the specific role of tight junction (TJ) associated proteins claudin-5, occludin, and ZO-1 as well as protein kinase C delta (PKCδ) pathway in BBB leakage induced by reperfusion injury. We verified a bimodal increase in the permeability of the BBB following focal ischemia by Evans blue assay. Two peaks of BBB permeability appeared at 3 h and 72 h of reperfusion after 2 h focal ischemia, respectively. The leak at the endothelial cell was represented at the level of transmission electron microscopy. TTC staining results showed increased infarct size with time after cerebral ischemia reperfusion. The mRNA and ...
The tight junctions (TJ), which are located in the apical region between epithelial and endothelial cells, regulate the paracellular diffusion of ions and small molecules and play an important role in maintaining cell polarity, cell-cell integrity, and permeability. In the lung, epithelial cells are attached by TJ structures. They provide a permeable barrier and cell communication. The loss of barrier integrity, which is maintained by the expression of claudins (Cldn), results in cellular permibilization and leads to paracellular diffusion of solutes and harmful molecules. There are 27 known Cldn homologous members in mice and human. Cldn6 is mostly expressed in embryonic stem cells and associated with the programing of epithelial cells during embryo development and lung morphogenesis. In order to test the hypothesis that Cldn6 expression affects lung morphogenesis, we analyzed the expression pattern of Cldn6 during lung ontogenesis to examine cell-specific expression pattern of Cldn6 during each
Mouse monoclonal ZO1 tight junction protein antibody [mAbcam 61357] validated for WB, IP, Flow Cyt and tested in Human. Referenced in 2 publications and 5…
Epithelial and endothelial cells form the external lining of outer and inner body surfaces and blood vessels of multicellular organisms. Thus, they create separate compartments each exhibiting an environment optimally adjusted to their respective function. To build up such compartments epithelial and endothelial cells have to restrict the paracellular diffusion of substances. The paracellular cleft is sealed by tight junctions (TJ). In electron microscopical images TJs appear as a network of intermembranous strands in the apical region of the lateral cell membrane of epithelial and endothelial cells. Claudins (Cld) form the structural backbone of TJs. The present study provided evidence for the first time that single amino acids of the second extracellular loop (ECL) of a claudin are essential for the paracellular tightness of epithelial cells. The effect of single amino acid substitutions of the second ECL of Cld5 were studied in cells expressing various other endogenous claudins except Cld5. ...
existed a side-stream of a far more modest character. It was a route which had been. and was married to his cousin, Gunhild Terjesdatter Haaland, in 1827 in Landvik Church. The couple settled at Enge in. Olsen Rise and Mikkel Bentsen Veding from Øyestad, Gjermund Jonsen Tøra from Fjære, and Halvor Kittelsen.. Täckström, Gerd Gunhild Margareta in Mölle, reviews by real people. Yelp is a fun and easy way to find, recommend and talk about whats great and not so great in.. Gunhild Friesia, f. 1882 Vs, lärarinna i Brum- merska skolan (S t o c k h o 1 m). Arthur Elof, f. 1837, handlande, t 1900. G. 1867 m. Jenny Malmberg, f. 1843. 1876 %, disponent för ång- bryggeriaktl). Svea i F i 1 i p s t a d. O. 1905 ^*A, m. Hilma Johanna Gyllenberg, f. 1879 Döttrar: Ingrid, f. 1906 %. Gerd, f. 1909 ^j-s. Astrid.. Sure, we all know we need to get cancer screenings periodically. But in between doctors appointments, we often forget to pay attention to what our bodies may be trying to tell us. Here, Dr. ...
Expression of CLDN4 (CPE-R, CPETR, CPETR1, hCPE-R, WBSCR8) in rectum tissue. Antibody staining with CAB002610 in immunohistochemistry.
The tight junction regulates passage of molecules throuth the paracellular spaces. Occludin and claudins are the specific trancmembrance protains present at the tight junction and are believed to regulate the cell barrier functions. To examine the response of the tight junction to hyperosmotic solutions, Ⅰinvestigated the effects of hyperosmotic glycerol on function and protein expression of the tight junction in ECV304 cells. Cell cytotoxicity analysis showed that the high (10%) concentration of glcerol damaged 64.1% of the ECV304 cells (p<0.001), and this was confirmed morphologically. Treatment with 1%, 2% or 5% glyserol increased the paracellular permeability of fluorescein isothiocyanate (FITC) -labeled dextran by 4.7%, 18.7% and 29.4% (p<0.05), respectively. In addition, exposure to glycerol at any concentration strongly reduced the expression of occludin, whereas enpression of claudin-1 was affected very slightly. These results suggest that hyperosmotic glycerol would certainly ...
Findings: hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture ...
Severn Trent Water, which currently recycles 100% of its biosolids to agriculture, is researching with Aston University intermediate rate gasification of biosolids as a strategic contingency if the EU should be so foolish as to adopt something like the sludge and biowaste working document. Sticky tar has been a problem with gasification, which it is hoped would be overcome by intermediate rate gasification at 450 C. The oil product would be used in de-tuned CHP engines. It is currently at proof of concept but might not go to full scale. STW is also looking at a novel aerator that could cut the financial and carbon cost of activate sludge. It will probably install side-stream treatment of dewatering liquor to recover (or remove) nitrogen and phosphate. Bluewater Bios HYBACS (developed in Korea) , which is an evolution of RBC that promotes growth of bacillus that will degrade organic micropollutants and reduce the whole life cost of activated sludge are being researched as are anaerobic ...
Over 125 genetic components in a chemotherapy-resistant, brain tumor-derived cell line were identified by University of Pittsburgh Cancer Institute (UPCI) researchers.
Expression of CLDN7 (CEPTRL2, CPETRL2, Hs.84359) in tonsil tissue. Antibody staining with HPA014703 and CAB013063 in immunohistochemistry.
SAN DIEGO, Jan. 29, 2014 (GLOBE NEWSWIRE) -- Celladon Corporation (Nasdaq:CLDN), a clinical-stage biotechnology company focused on developing novel therapies by applying its leadership position in the field of SERCA enzymes, announced today the pricing of its initial public offering of 5,500,000 shares of its common stock at $8.00 per share.

Claudin-3 (mouse)Claudin-3 (mouse)

The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and ...
more infohttps://www.phosphosite.org/proteinAction.action?id=7569&showAllSites=true

CLDN3 - Claudin-3 - Homo sapiens (Human) - CLDN3 gene & proteinCLDN3 - Claudin-3 - Homo sapiens (Human) - CLDN3 gene & protein

IPR006187 Claudin. IPR003549 Claudin3. IPR017974 Claudin_CS. IPR004031 PMP22/EMP/MP20/Claudin. ... IPR006187 Claudin. IPR003549 Claudin3. IPR017974 Claudin_CS. IPR004031 PMP22/EMP/MP20/Claudin. ... Belongs to the claudin family.Curated. Keywords - Domaini. Transmembrane, Transmembrane helix. Phylogenomic databases. ... sp,O15551,CLD3_HUMAN Claudin-3 OS=Homo sapiens OX=9606 GN=CLDN3 PE=1 SV=1 ...
more infohttps://www.uniprot.org/uniprot/O15551

Generierung und Charakterisierung ei-nes Claudin-3-defizienten MausmodellsGenerierung und Charakterisierung ei-nes Claudin-3-defizienten Mausmodells

Claudin-3 (CLDN3) is a ubiquitously expressed TJ protein, which functional role in vivo is still unknown. To gain insight into ... Claudin-3 (CLDN3) ist ein ubiquitär exprimiertes TJ-Protein, dessen Rolle in vivo jedoch unbekannt ist. Um Einblicke in dessen ... wurde für diese Arbeit ein Claudin-3-defizientes Mausmodell mittels der konditionalen Gentargeting-Technologie generiert. Zur ... its physiological function a claudin-3 deficient mouse model has been generated using the conditional gene targeting technology ...
more infohttps://edoc.hu-berlin.de/handle/18452/17423

Probing the cis-arrangement of prototype tight junction proteins claudin-1 and claudin-3.  - PubMed - NCBIProbing the cis-arrangement of prototype tight junction proteins claudin-1 and claudin-3. - PubMed - NCBI

3):449-58. doi: 10.1042/BJ20150148. Epub 2015 Apr 7. Comparative Study; Research Support, Non-U.S. Govt ... Human claudin-1 and claudin-3, fused to ECFP or EYFP at the N- or C-terminus, were expressed in the TJ-free cell line HEK ( ... Probing the cis-arrangement of prototype tight junction proteins claudin-1 and claudin-3.. Milatz S1, Piontek J2, Schulzke JD2 ... Using FRET analysis, the proximity of claudin N- and C-termini integrated in homopolymeric strands composed of claudin-3 or of ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/25849148

IJMS | Free Full-Text | SCF/C-Kit/JNK/AP-1 Signaling Pathway Promotes Claudin-3 Expression in Colonic Epithelium and Colorectal...IJMS | Free Full-Text | SCF/C-Kit/JNK/AP-1 Signaling Pathway Promotes Claudin-3 Expression in Colonic Epithelium and Colorectal...

In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant ... Furthermore, decreased expression of claudin-3 was obtained in the colonic epithelium from the c-Kit loss-of-function mutant ... Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression ... We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon ...
more infohttp://www.mdpi.com/1422-0067/18/4/765

anti-CLDN3 antibody | Rabbit CLDN3 / Claudin 3 Polyclonal Antibody-NP 001297.1anti-CLDN3 antibody | Rabbit CLDN3 / Claudin 3 Polyclonal Antibody-NP 001297.1

Claudin 3 Polyclonal Antibody-NP_001297.1 (MBS241994) product datasheet at MyBioSource, Primary Antibodies. Application: ... Anti-CLDN3 / Claudin 3 Antibody (C-Terminus) IHC-plus; CLDN3; C7orf1; Claudin-3; CPE-R 2; Claudin 3; RVP1; CPE-R2; CPE-receptor ... CLDN3 / Claudin 3 antibody was raised against a synthetic peptide derived from the C-terminus of mouse Claudin 3. Cellular ... 1. Claudin 3 was expressed in all non-goblet columnar lined oesophagus, Barretts oesophagus, high grade dysplasia and ...
more infohttps://www.mybiosource.com/polyclonal-human-mouse-rat-antibody/cldn3-claudin-3/241994

Claudin-3 Inhibits Lung Squamous Cell Carcinoma Cell Epithelial-mesenchymal Transition and Invasion via Suppression of the Wnt...Claudin-3 Inhibits Lung Squamous Cell Carcinoma Cell Epithelial-mesenchymal Transition and Invasion via Suppression of the Wnt...

Claudin-1 Protein Expression Is a Good Prognositic Factor in Non-Small Cell Lung Cancer, but only in Squamous Cell Carcinoma ... Role of claudin interactions in airway tight junctional permeability. Am J Physiol Lung Cell Mol Physiol. 2003;285:L1166-78 ... Role of claudin interactions in airway tight junctional permeability. Am J Physiol Lung Cell Mol Physiol. 2003;285:L1166-78 ... Decreased expression of claudin-3 is associated with a poor prognosis and EMT in completely resected squamous cell lung ...
more infohttp://www.medsci.org/v15p0339.htm

Anti-Claudin 3 抗体 (ab116165) | アブカムAnti-Claudin 3 抗体 (ab116165) | アブカム

"ウサギ・ポリクローナル抗体 ab116165 交差種: Hu 適用: WB,IHC-P…Claudin 3抗体一覧…画像、プロトコール、文献などWeb上の情報 ... All lanes : Anti-Claudin 3 antibody (ab116165) at 1 µg/ml. Lane 1 : Human ovary tissue lysate - total protein (ab30222). Lane 2 ... Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Claudin 3. Immunogen の所有権に関して ... IHC image of Claudin 3 staining in Human normal prostate formalin fixed paraffin
more infohttp://www.abcam.co.jp/claudin-3-antibody-ab116165.html

Porcine CLDN3 (Claudin 3) ELISA Kit  Manufacturers in DelhiPorcine CLDN3 (Claudin 3) ELISA Kit Manufacturers in Delhi

Claudin 3) ELISA Kit OSCAR DIAGNOSTIC SERVICES PVT. LTD.is an India based Company in Delhi. ... Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit ... Porcine CLDN3 (Claudin 3) ELISA Kit » Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 ( ... Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 ( ...
more infohttps://www.odspl.com/sub-product/Porcine+CLDN3+%28Claudin+3%29+ELISA+Kit+.php

Association of Cytokeratin 5 and Claudin 3 expression with BRCA1 and BRCA2 germline mutations in women with early breast cancer...Association of Cytokeratin 5 and Claudin 3 expression with BRCA1 and BRCA2 germline mutations in women with early breast cancer...

Claudin (CLDN) 3. Positive expression in BRCA1 breast cancer (BC) (a) and negative control (isotypic antibody) (b). Positive ... Thus, in this study, we examined the protein expression of claudin (CLDN) 3, CLDN4, CLDN7, and E-cadherin. Moreover, we ... Association of Cytokeratin 5 and Claudin 3 expression with BRCA1 and BRCA2 germline mutations in women with early breast cancer ... Association of Cytokeratin 5 and Claudin 3 expression with BRCA1 and BRCA2 germline mutations in women with early breast cancer ...
more infohttps://phgkb.cdc.gov/PHGKB/phgHome.action?action=forward&dbsource=huge&id=176568

Sorry, ab15103 is not availableSorry, ab15103 is not available

Sorry, Anti-Claudin 3 antibody, prediluted (ab15103) is not available ab15103 is not available and we regret any inconvenience ...
more infohttps://www.abcam.com/claudin-3-antibody-prediluted-ab15103.html

Search of: Recruiting, Not yet recruiting, Available Studies | Nutrition - List Results - ClinicalTrials.govSearch of: Recruiting, Not yet recruiting, Available Studies | 'Nutrition' - List Results - ClinicalTrials.gov

Claudin-3. 150. All. 1 Month to 16 Years (Child). NCT02598375. ERTEN-IFABP IN PICU. ERTENIFABPICU. January 2015. December 2016 ... and 3 more...). 70. All. 20 Years and older (Adult, Senior). NCT02159456. feed520. May 2014. April 2017. April 2017. June 10, ... and 3 more...). 50. All. 18 Years and older (Adult, Senior). NCT02796833. 14-8537-A. March 2016. December 2018. December 2018. ... and 3 more...). 3782. All. 18 Years and older (Adult, Senior). NCT03284398. 3CBs-001-CNI. December 14, 2017. November 2018. ...
more infohttps://clinicaltrials.gov/ct2/results?recr=Open&cond=%22Nutrition%22&show_rss=Y&sel_rss=mod14

Michael J. Becich, MD, PhD | Department of Biomedical InformaticsMichael J. Becich, MD, PhD | Department of Biomedical Informatics

Immunohistochemical profiles of claudin-3 in primary and metastatic prostatic adenocarcinoma. Bartholow TL, Chandran UR, Becich ... Immunohistochemical profiles of claudin-3 in primary and metastatic prostatic adenocarcinoma. Diagn Pathol. 2011 Jan 21;6:12. ... 2011 Jun 1;121(3):586-94. Epub 2011 Apr 1. PMID: 21458040 PubMed Journal - in process ...
more infohttps://www.dbmi.pitt.edu/publications/2?page=2

Uma Chandran, PhD, MSIS  | Department of Biomedical InformaticsUma Chandran, PhD, MSIS | Department of Biomedical Informatics

Immunohistochemical profiles of claudin-3 in primary and metastatic prostatic adenocarcinoma. Bartholow TL, Chandran UR, Becich ... Immunohistochemical profiles of claudin-3 in primary and metastatic prostatic adenocarcinoma. Diagn Pathol. 2011 Jan 21;6:12. ... 2008 Mar;179(3):892-5; discussion 895. PMID: 18207193 DOI: 10.1016/j.juro.2007.10.057 ...
more infohttps://www.dbmi.pitt.edu/publications/1214?page=3

Gene Expression Literature DetailGene Expression Literature Detail

claudin 3. MGI:1329044 Reference. J:178498 Fagman H, Amendola E, Parrillo L, Zoppoli P, Marotta P, Scarfo M, De Luca P, de ...
more infohttp://www.informatics.jax.org/gxdlit/key/136359

KEGG PATHWAY: hsa04530KEGG PATHWAY: hsa04530

TJs are composed of at least three types of transmembrane protein -occludin, claudin and junctional adhesion molecules (JAMs)- ... DLG3; discs large MAGUK scaffold protein 3 [KO:K21098]. 4734 NEDD4; NEDD4 E3 ubiquitin protein ligase [KO:K10591] [EC:2.3.2.26] ... MARVELD3; MARVEL domain containing 3 [KO:K21099]. 4214 MAP3K1; mitogen-activated protein kinase kinase kinase 1 [KO:K04416] [EC ... ACTR3; actin related protein 3 [KO:K18584]. 123720 WHAMM; WASP homolog associated with actin, golgi membranes and microtubules ...
more infohttps://www.genome.jp/dbget-bin/www_bget?pathway+hsa04530

KEGG PATHWAY: hsa04514KEGG PATHWAY: hsa04514

Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development. ... HLA-DRB3; major histocompatibility complex, class II, DR beta 3 [KO:K06752] ... Mol Cell Biol Res Commun 3:255-63 (2000). DOI:10.1006/mcbr.2000.0225 ... CLDN4; claudin 4 [KO:K06087]. 1365 CLDN3; claudin 3 [KO:K06087]. 1366 ...
more infohttp://www.genome.jp/dbget-bin/www_bget?pathway+hsa04514

Gene Expression Literature Summary - MGIGene Expression Literature Summary - MGI

J:165127 Thompson PD, Tipney H, Brass A, Noyes H, Kemp S, Naessens J, Tassabehji M, Claudin 13, a member of the claudin family ... J:181271 Westmoreland JJ, Drosos Y, Kelly J, Ye J, Means AL, Washington MK, Sosa-Pineda B, Dynamic distribution of claudin ... Probiotic bacteria induce maturation of intestinal claudin 3 expression and barrier function. Am J Pathol. 2012 Feb;180(2):626- ... Predicted expansion of the claudin multigene family. FEBS Lett. 2011 Feb 18;585(4):606-12 ...
more infohttp://www.informatics.jax.org/gxdlit/summary/ageAssay?age=P&assayType=RT-PCR&year=&nomen=&journal=&text=&inAbstract=true&inTitle=true&author=&authorScope=any&marker_key=40428

AMICA/JAML Antibody (4E10) [Alexa Fluor® 488] (NBP1-43309AF488): Novus BiologicalsAMICA/JAML Antibody (4E10) [Alexa Fluor® 488] (NBP1-43309AF488): Novus Biologicals

Armenian Hamster Monoclonal Anti-AMICA/JAML Antibody (4E10) [Alexa Fluor® 488]. Validated: Flow. Tested Reactivity: Mouse. 100% Guaranteed.
more infohttps://www.novusbio.com/products/amica-jaml-antibody-4e10_nbp1-43309af488

AMICA/JAML Antibody (4E10) [Alexa Fluor® 405] (NBP1-43309AF405): Novus BiologicalsAMICA/JAML Antibody (4E10) [Alexa Fluor® 405] (NBP1-43309AF405): Novus Biologicals

Armenian Hamster Monoclonal Anti-AMICA/JAML Antibody (4E10) [Alexa Fluor® 405]. Validated: Flow. Tested Reactivity: Mouse. 100% Guaranteed.
more infohttps://www.novusbio.com/products/amica-jaml-antibody-4e10_nbp1-43309af405

View source for Pagets disease of the breast pathophysiology - wikidocView source for Paget's disease of the breast pathophysiology - wikidoc

Claudin 4 , style="background: #F5F5F5; padding: 5px;" ,100% ,- , style="background: #F5F5F5; padding: 5px;" ,Claudin 5 , style ... Claudin 2]] , style="background: #F5F5F5; padding: 5px;" ,32% ,- , style="background: #F5F5F5; padding: 5px;" ,[[Claudin 3]] , ... Her 3]] and [[Her 4]] which are produced by Pagets cells. This is thought to result in migration of these [[cells]] to the [[ ... 3,601::AID-CNCR2820620326>3.0.CO;2-7}},/ref>. *The number of [[cells]] vary from a few to large quantities; even completely ...
more infohttp://wikidoc.org/index.php?title=Paget%27s_disease_of_the_breast_pathophysiology&action=edit

Cldn3 - PrimePCR Assay and Template | Life Science | Bio-RadCldn3 - PrimePCR Assay and Template | Life Science | Bio-Rad

Claudin 3 Assay Type: Probe Assay Design: Exonic Application: Gene Expression Unique Assay ID: qMmuCEP0031959 FAM HEX TEX 615 ... Claudin 3 Assay Type: SYBR® Green Assay Design: Exonic Application: Gene Expression Unique Assay ID: qMmuCED0001019 Info: Same ... Claudin 3 Assay Type: EvaGreen Application: Gene Expression Unique Assay ID: dMmuEG5059387 Info: EG; Same primer pair as used ... Claudin 3 Assay Type: Probe Application: Gene Expression Unique Assay ID: dMmuCPE5088998 Info: FAM; Same primer pair and probe ...
more infohttp://www.bio-rad.com/en-us/prime-pcr-assays/gene/cldn3-mouse

Human Metabolome Database: Browsing ProteinsHuman Metabolome Database: Browsing Proteins

Claudin-3. Unknown. 1 metabolite. HMDBP07863. PLA2G4D. 15q15.1. Cytosolic phospholipase A2 delta. Unknown. 1088 metabolites. ... 3 metabolites. HMDBP00869. MAN1B1. 9q34. Endoplasmic reticulum mannosyl-oligosaccharide 1,2-alpha-mannosidase. Unknown. 4 ... Solute carrier family 23 member 3. Unknown. 1 metabolite. HMDBP11810. MAN2B1. 19cen-q13.1. Lysosomal alpha-mannosidase. Unknown ... Cyclic AMP-responsive element-binding protein 3-like protein 1. Unknown. 1 metabolite. ...
more infohttp://www.hmdb.ca/proteins?c=protein_type&d=down&page=4

ENSDARG00000069503 - Zebrafish Mutation Project - Wellcome Sanger InstituteENSDARG00000069503 - Zebrafish Mutation Project - Wellcome Sanger Institute

claudin 3 [Source:HGNC Symbol;Acc:2045]. Mouse Orthologue:. Cldn3. Mouse Description:. claudin 3 Gene [Source:MGI Symbol;Acc: ... claudin-3 [Source:RefSeq peptide;Acc:NP_571842]. Human Orthologue:. CLDN3. Human Description:. ...
more infohttps://www.sanger.ac.uk/sanger/Zebrafish_Zmpgene/ENSDARG00000069503

The alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate receptor trafficking regulator stargazin is related to the claudin...The alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate receptor trafficking regulator "stargazin" is related to the claudin...

The graph plots aggregation by the particle number at a time point divided by the particle number at time 0 (Nt/No). Claudin-1 ... PKH26-labeled untransfected L-cells (red) were mixed 1:1 with unlabeled untransfected L-cells (top row), GFP-Claudin-1- ... Expression of Claudin-1 or Cacng2 promotes cell aggregation, as shown by microscopy and particle counts. ... Because stargazin is structurally similar to claudins, we hypothesized that it might also have retained claudin-like functions ...
more infohttps://www.ncbi.nlm.nih.gov/pubmed/15760900?dopt=Abstract
  • The main results are that (i) within homo- and heteropolymers, the average distance between the cytoplasmic ends of the TM1s of cis-interacting claudin molecules is shorter than the average distance between their TM4s, and (ii) TM1 segments of neighbouring claudins are oriented towards each other as the cytoplasmic end of TM1 is in close proximity to more other TM1 segments than TM4 is to other TM4 segments. (nih.gov)
  • Because stargazin is structurally similar to claudins, we hypothesized that it might also have retained claudin-like functions inherited from a common ancestor. (nih.gov)
  • A , the relationship of stargazin (Cacng2) and other Cacng proteins to the claudins is shown in a phylogenetic tree inferred from the amino acid alignment of the 33 indicated human proteins: Clarin-1, Cacng1-8, EMP1-3, PMP-22, LIM2, and 20 claudins (CLDN). (nih.gov)
  • We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. (mdpi.com)
  • Human claudin-1 and claudin-3, fused to ECFP or EYFP at the N- or C-terminus, were expressed in the TJ-free cell line HEK (human embryonic kidney)-293. (nih.gov)
  • In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. (mdpi.com)
  • Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Claudin 3. (abcam.co.jp)
  • There are over 3 million human Expressed Sequence Tag (EST) records in GenBank (Table 1 ), which is still growing rapidly. (biomedcentral.com)
  • J:180511 Patel RM, Myers LS, Kurundkar AR, Maheshwari A, Nusrat A, Lin PW, Probiotic bacteria induce maturation of intestinal claudin 3 expression and barrier function. (jax.org)
  • Association of Cytokeratin 5 and Claudin 3 expression with BRCA1 and BRCA2 germline mutations in women with early breast cancer. (cdc.gov)
  • A synthetic peptide derived from the C-terminus of mouse Claudin 3. (mybiosource.com)
  • http://doi.org/10.1186/1471-2105-12-21 3. (pitt.edu)
  • Claudin-3: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. (mybiosource.com)
  • Che J, Yue D, Zhang B, Zhang H, Huo Y, Gao L, Zhen H, Yang Y, Cao B. Claudin-3 Inhibits Lung Squamous Cell Carcinoma Cell Epithelial-mesenchymal Transition and Invasion via Suppression of the Wnt/β-catenin Signaling Pathway. (medsci.org)
  • 3 , 7 This is particularly true after repeated passages because cell spreading and proliferation are known to be inversely related to differentiation. (arvojournals.org)
  • Bartholow TL, Chandran UR , Becich MJ , Parwani AV. Immunohistochemical profiles of claudin-3 in primary and metastatic prostatic adenocarcinoma. (pitt.edu)
  • Using FRET analysis, the proximity of claudin N- and C-termini integrated in homopolymeric strands composed of claudin-3 or of heteropolymeric strands composed of claudin-1 and claudin-3 were determined. (nih.gov)
  • ARPE-19 cells at passages p22 to p28 were cultured on filters for up to 3 months in DMEM/F12 or DMEM media with or without pyruvate and 1% fetal calf serum. (arvojournals.org)