A large family of transmembrane proteins found in TIGHT JUNCTIONS. They take part in the formation of paracellular barriers and pores that regulate paracellular permeability.
A ubiquitously-expressed claudin subtype that acts as a general barrier-forming protein in TIGHT JUNCTIONS. Elevated expression of claudin-3 is found in a variety of tumor cell types, suggesting its role as a therapeutic target for specific ANTINEOPLASTIC AGENTS.
An integral membrane protein that is localized to TIGHT JUNCTIONS, where it plays a role in controlling the paracellular permeability of polarized cells. Mutations in the gene for claudin-1 are associated with Neonatal Ichthyosis-Sclerosing Cholangitis (NISCH) Syndrome.
A claudin subtype that takes part in maintaining the barrier-forming property of TIGHT JUNCTIONS. Claudin-4 is found associated with CLAUDIN-8 in the KIDNEY COLLECTING DUCT where it may play a role in paracellular chloride ion reabsorption.
Cell-cell junctions that seal adjacent epithelial cells together, preventing the passage of most dissolved molecules from one side of the epithelial sheet to the other. (Alberts et al., Molecular Biology of the Cell, 2nd ed, p22)
A claudin subtype that is found localized to TIGHT JUNCTIONS in VASCULAR ENDOTHELIAL CELLS. The protein was initially identified as one of several proteins which are deleted in VELOCARDIOFACIAL SYNDROME and may play an important role in maintaining the integrity of the BLOOD-BRAIN BARRIER.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A MARVEL domain protein that plays an important role in the formation and regulation of the TIGHT JUNCTION paracellular permeability barrier.
A 195-kDa zonula occludens protein that is distinguished by the presence of a ZU5 domain at the C-terminal of the molecule.
A zonula occludens protein subtype found in epithelial cell junctions. Several isoforms of zonula occludens-2 protein exist due to use of alternative promoter regions and alternative mRNA splicings.
A claudin subtype that is associated with the formation of cation-selective channels and increased epithelial permeability. It is localized to the TIGHT JUNCTIONS of the PROXIMAL KIDNEY TUBULE and INTESTINAL EPITHELIUM.
A specialized barrier, in the TESTIS, between the interstitial BLOOD compartment and the adluminal compartment of the SEMINIFEROUS TUBULES. The barrier is formed by layers of cells from the VASCULAR ENDOTHELIUM of the capillary BLOOD VESSELS, to the SEMINIFEROUS EPITHELIUM of the seminiferous tubules. TIGHT JUNCTIONS form between adjacent SERTOLI CELLS, as well as between the ENDOTHELIAL CELLS.
Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.
The resistance to the flow of either alternating or direct electrical current.
The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.
A genus of pufferfish commonly used for research.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Excretion of abnormally high level of CALCIUM in the URINE, greater than 4 mg/kg/day.
An inherited renal disorder characterized by defective NaCl reabsorption in the convoluted DISTAL KIDNEY TUBULE leading to HYPOKALEMIA. In contrast with BARTTER SYNDROME, Gitelman syndrome includes hypomagnesemia and normocalcemic hypocalciuria, and is caused by mutations in the thiazide-sensitive SODIUM-POTASSIUM-CHLORIDE SYMPORTERS.
A condition characterized by calcification of the renal tissue itself. It is usually seen in distal RENAL TUBULAR ACIDOSIS with calcium deposition in the DISTAL KIDNEY TUBULES and the surrounding interstitium. Nephrocalcinosis causes RENAL INSUFFICIENCY.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.
The U-shaped portion of the renal tubule in the KIDNEY MEDULLA, consisting of a descending limb and an ascending limb. It is situated between the PROXIMAL KIDNEY TUBULE and the DISTAL KIDNEY TUBULE.
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
Cells of epithelial origin possessing specialized sensory functions. They include cells that are found in the TASTE BUDS; OLFACTORY MUCOSA; COCHLEA; and NEUROEPITHELIAL BODIES.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Anchoring points where the CYTOSKELETON of neighboring cells are connected to each other. They are composed of specialized areas of the plasma membrane where bundles of the ACTIN CYTOSKELETON attach to the membrane through the transmembrane linkers, CADHERINS, which in turn attach through their extracellular domains to cadherins in the neighboring cell membranes. In sheets of cells, they form into adhesion belts (zonula adherens) that go all the way around a cell.
Supporting cells projecting inward from the basement membrane of SEMINIFEROUS TUBULES. They surround and nourish the developing male germ cells and secrete ANDROGEN-BINDING PROTEIN and hormones such as ANTI-MULLERIAN HORMONE. The tight junctions of Sertoli cells with the SPERMATOGONIA and SPERMATOCYTES provide a BLOOD-TESTIS BARRIER.
A usually benign glandular tumor composed of oxyphil cells, large cells with small irregular nuclei and dense acidophilic granules due to the presence of abundant MITOCHONDRIA. Oxyphil cells, also known as oncocytes, are found in oncocytomas of the kidney, salivary glands, and endocrine glands. In the thyroid gland, oxyphil cells are known as Hurthle cells and Askanazy cells.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)
Established cell cultures that have the potential to propagate indefinitely.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Degree of saltiness, which is largely the OSMOLAR CONCENTRATION of SODIUM CHLORIDE plus any other SALTS present. It is an ecological factor of considerable importance, influencing the types of organisms that live in an ENVIRONMENT.
The simultaneous analysis of multiple samples of TISSUES or CELLS from BIOPSY or in vitro culture that have been arranged in an array format on slides or microchips.
The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A general term for the complete or partial loss of the ability to hear from one or both ears.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
The part of the inner ear (LABYRINTH) that is concerned with hearing. It forms the anterior part of the labyrinth, as a snail-like structure that is situated almost horizontally anterior to the VESTIBULAR LABYRINTH.
Phenotypic changes of EPITHELIAL CELLS to MESENCHYME type, which increase cell mobility critical in many developmental processes such as NEURAL TUBE development. NEOPLASM METASTASIS and DISEASE PROGRESSION may also induce this transition.
The portion of renal tubule that begins from the enlarged segment of the ascending limb of the LOOP OF HENLE. It reenters the KIDNEY CORTEX and forms the convoluted segments of the distal tubule.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.
Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.
A malignant neoplasm arising from tenosynovial tissue of the joints and in synovial cells of tendons and bursae. The legs are the most common site, but the tumor can occur in the abdominal wall and other trunk muscles. There are two recognized types: the monophasic (characterized by sheaths of monotonous spindle cells) and the biphasic (characterized by slit-like spaces or clefts within the tumor, lined by cuboidal or tall columnar epithelial cells). These sarcomas occur most commonly in the second and fourth decades of life. (From Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1363)
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The mucous membrane lining the RESPIRATORY TRACT, including the NASAL CAVITY; the LARYNX; the TRACHEA; and the BRONCHI tree. The respiratory mucosa consists of various types of epithelial cells ranging from ciliated columnar to simple squamous, mucous GOBLET CELLS, and glands containing both mucous and serous cells.
Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
An EPITHELIUM with MUCUS-secreting cells, such as GOBLET CELLS. It forms the lining of many body cavities, such as the DIGESTIVE TRACT, the RESPIRATORY TRACT, and the reproductive tract. Mucosa, rich in blood and lymph vessels, comprises an inner epithelium, a middle layer (lamina propria) of loose CONNECTIVE TISSUE, and an outer layer (muscularis mucosae) of SMOOTH MUSCLE CELLS that separates the mucosa from submucosa.
Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The sequential location of genes on a chromosome.
Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place.
Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
A carcinoma discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass. (From Cancer Chemother Rep 2 1972 Nov;(3)1:325) It is also called 3LL and LLC and is used as a transplantable malignancy.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
A cell line derived from cultured tumor cells.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.
Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Refers to animals in the period of time just after birth.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
The relationships of groups of organisms as reflected by their genetic makeup.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
MAMMARY GLANDS in the non-human MAMMALS.
A nonparametric method of compiling LIFE TABLES or survival tables. It combines calculated probabilities of survival and estimates to allow for observations occurring beyond a measurement threshold, which are assumed to occur randomly. Time intervals are defined as ending each time an event occurs and are therefore unequal. (From Last, A Dictionary of Epidemiology, 1995)
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Tumors or cancer of the human BREAST.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
Adherence of cells to surfaces or to other cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Laboratory tests used to evaluate how well the kidneys are working through examination of blood and urine.
Proteins prepared by recombinant DNA technology.

Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions. (1/114)

In multicellular organisms, various compositionally distinct fluid compartments are established by epithelial and endothelial cellular sheets. For these cells to function as barriers, tight junctions (TJs) are considered to create a primary barrier for the diffusion of solutes through the paracellular pathway [1] [2] [3]. In ultrathin sections viewed under electron microscopy, TJs appear as a series of apparent fusions, involving the outer leaflets of plasma membranes of adjacent cells, to form the so-called kissing points of TJs, where the intercellular space is completely obliterated [4]. Claudins are a family of 16 proteins whose members have been identified as major integral membrane proteins localized exclusively at TJs [5] [6] [7] [8]. It remains unclear, however, whether claudins have the cell-adhesion activity that would explain the unusual intercellular adhesion at TJs. Using mouse L-fibroblast transfectants expressing various amounts of claudin-1, -2 or -3, we found that these claudins possess Ca(2+)-independent cell-adhesion activity. Using ultrathin-section electron microscopy, we observed many kissing points of TJs between adjacent transfectants. Furthermore, the cell-adhesion activity of occludin, another integral membrane protein localized at TJs [9] [10] [11], was negligible when compared with that of claudins. Thus, claudins are responsible for TJ-specific obliteration of the intercellular space.  (+info)

Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: Evidence for direct involvement of claudins in tight junction barrier. (2/114)

Claudins, comprising a multigene family, constitute tight junction (TJ) strands. Clostridium perfringens enterotoxin (CPE), a single approximately 35-kD polypeptide, was reported to specifically bind to claudin-3/RVP1 and claudin-4/CPE-R at its COOH-terminal half. We examined the effects of the COOH-terminal half fragment of CPE (C-CPE) on TJs in L transfectants expressing claudin-1 to -4 (C1L to C4L, respectively), and in MDCK I cells expressing claudin-1 and -4. C-CPE bound to claudin-3 and -4 with high affinity, but not to claudin-1 or -2. In the presence of C-CPE, reconstituted TJ strands in C3L cells gradually disintegrated and disappeared from their cell surface. In MDCK I cells incubated with C-CPE, claudin-4 was selectively removed from TJs with its concomitant degradation. At 4 h after incubation with C-CPE, TJ strands were disintegrated, and the number of TJ strands and the complexity of their network were markedly decreased. In good agreement with the time course of these morphological changes, the TJ barrier (TER and paracellular flux) of MDCK I cells was downregulated by C-CPE in a dose-dependent manner. These findings provided evidence for the direct involvement of claudins in the barrier functions of TJs.  (+info)

Manner of interaction of heterogeneous claudin species within and between tight junction strands. (3/114)

In tight junctions (TJs), TJ strands are associated laterally with those of adjacent cells to form paired strands to eliminate the extracellular space. Claudin-1 and -2, integral membrane proteins of TJs, reconstitute paired TJ strands when transfected into L fibroblasts. Claudins comprise a multigene family and more than two distinct claudins are coexpressed in single cells, raising the questions of whether heterogeneous claudins form heteromeric TJ strands and whether claudins interact between each of the paired strands in a heterophilic manner. To answer these questions, we cotransfected two of claudin-1, -2, and -3 into L cells, and detected their coconcentration at cell-cell borders as elaborate networks. Immunoreplica EM confirmed that distinct claudins were coincorporated into individual TJ strands. Next, two L transfectants singly expressing claudin-1, -2, or -3 were cocultured and we found that claudin-3 strands laterally associated with claudin-1 and -2 strands to form paired strands, whereas claudin-1 strands did not interact with claudin-2 strands. We concluded that distinct species of claudins can interact within and between TJ strands, except in some combinations. This mode of assembly of claudins could increase the diversity of the structure and functions of TJ strands.  (+info)

Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane protein. (4/114)

Claudins (claudin-1 to -18) with four transmembrane domains and two extracellular loops constitute tight junction strands. The peptide toxin Clostridium perfringens enterotoxin (CPE) has been shown to bind to claudin-3 and -4, but not to claudin-1 or -2. We constructed claudin-1/claudin-3 chimeric molecules and found that the second extracellular loop of claudin-3 conferred CPE sensitivity on L fibroblasts. Furthermore, overlay analyses revealed that the second extracellular loop of claudin-3 specifically bound to CPE at the K(a) value of 1.0x10(8) M(-1). We concluded that the second extracellular loop is the site through which claudin-3 interacts with CPE on the cell surface.  (+info)

Claudin promotes activation of pro-matrix metalloproteinase-2 mediated by membrane-type matrix metalloproteinases. (5/114)

Genes associated with regulation of membrane-type matrix metalloproteinase-1 (MT1-MMP)-mediated pro-MMP-2 processing were screened in 293T cells by a newly developed expression cloning method. One of the gene products, which promoted processing of pro-MMP-2 by MT1-MMP was claudin-5, a major component of endothelial tight junctions. Expression of claudin-5 not only replaced TIMP-2 in pro-MMP-2 activation by MT1-MMP but also promoted activation of pro-MMP-2 mediated by all MT-MMPs and MT1-MMP mutants lacking the transmembrane domain (DeltaMT1-MMP). A carboxyl-terminal deletion mutant of pro-MMP-2 (proDeltaMMP-2) was processed to an intermediate form by MT1-MMP in 293T cells and was further converted to an activated form by introduction of claudin-5. In contrast to the stimulatory effect of TIMP-2 on pro-MMP-2 activation by MT1-MMP, activation of pro-MMP-2 by DeltaMT1-MMP in the presence of claudin-5 and proDeltaMMP-2 processing by MT1-MMP were both inversely repressed by expression of exogenous TIMP-2. These results suggest that TIMP-2 is not involved in cluadin-5-induced pro-MMP-2 activation by MT-MMPs. Stimulation of MT-MMP-mediated pro-MMP-2 activation was also observed with other claudin family members, claudin-1, claudin-2, and claudin-3. Amino acid substitutions or deletions in ectodomain of claudin-1 abolished stimulatory effect. Direct interaction of claudin-1 with MT1-MMP and MMP-2 was demonstrated by immunoprecipitation analysis. MT1-MMP was co-localized with claudin-1 not only at cell-cell borders, but also at other parts of the cells. TIMP-2 enhanced cell surface localization of MMP-2 mediated by MT1-MMP, and claudin-1 also stimulated it. These results suggest that claudin recruits all MT-MMPs and pro-MMP-2 on the cell surface to achieve elevated focal concentrations and, consequently, enhances activation of pro-MMP-2.  (+info)

Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium. (6/114)

The mRNA for Rvp.1 (rat ventral prostate) increases in abundance before gland involution after androgen deprivation. Rvp.1 is homologous to CPE-R, the high-affinity intestinal epithelial receptor for Clostridium perfringens enterotoxin (CPE), and is sufficient to mediate CPE binding and trigger subsequent toxin-mediated cytolysis. Rvp.1 (claudin-3) and CPE-R (claudin-4) are members of a larger family of transmembrane tissue-specific claudin proteins that are essential components of intercellular tight junction structures regulating paracellular ion flux. However, claudin-3 and claudin-4 are the only family members capable of mediating CPE binding and cytolysis. The present study was designed to study the expression of claudin-3 and claudin-4 in human prostate tissue as potential targets for CPE toxin-mediated therapy for prostate cancer. On human multiple-tissue Northern blot analysis, mRNAs for both claudin-3 and claudin-4 were expressed at high levels in prostate tissue. In normal prostate tissue, expression of claudin-3 was localized exclusively within acinar epithelial cells by in situ mRNA hybridization. Compared with expression within prostate epithelial cells in surrounding normal glandular tissue, expression of claudin-3 mRNA remained high in the epithelium of prostate adenocarcinoma (10 of 10) and prostatic intraepithelial neoplasia (five of five). Prostate adenocarcinoma cells metastatic to bone were obtained from a patient with disease progression during antiandrogen therapy. These metastatic cells were prostate-specific antigen-positive by immunohistochemical staining and also expressed functional CPE receptors as measured by sensitivity to CPE-induced cell lysis. The persistent high level of claudin-3 expression in prostate adenocarcinoma and functional cytotoxicity of CPE in metastatic androgen-independent prostate adenocarcinoma suggests a new potential therapeutic strategy for prostate cancer.  (+info)

The renal segmental distribution of claudins changes with development. (7/114)

BACKGROUND: Permeability properties of mammalian nephron are tuned during postnatal maturation. The transepithelial electrical resistance (TER) and complexity of tight junctions (TJs) vary along the different tubular segments, suggesting that the molecules constituting this structure change. We studied the differential expression of occludin and several claudins in isolated renal tubules from newborn and adult rabbits. METHODS: Isolated renal tubules from newborn and adult rabbits were processed for occludin, claudin-1 and claudin-2 immunofluorescence, and Western blot detection of claudin-1 and -2. Claudin-5 was detected in whole kidney frozen sections. RT-PCR from isolated tubules was performed for claudins-1 to -8. RESULTS: Immunofluorescence revealed that occludin, claudin-1 and -2 were present at the cell boundaries at the neonatal stage of development. Claudin-1 was detected in the tighter segments of the nephron (distal and collecting duct), while claudin-2 was found in the leaky portions (proximal). Claudin 5 was found in the kidney vasculature. PCR amplification revealed the presence of claudins-1 to -4 in tubules of newborns. In adults, claudins-1, -2 and -4 were present in proximal, Henle's loop and collecting segments; claudin-3 was in proximal and collecting tubules, while claudins-5 and -6 were absent from all tubular portions. Claudin-7 was restricted to proximal tubules, while claudin-8 was present in proximal and Henle's segments. CONCLUSIONS: The pattern of occludin distribution is present from the neonatal age. Claudins-7 and -8 are up-regulated after birth. Each tubular segment expresses a peculiar set of claudins that might be responsible for the permeability properties of their TJs.  (+info)

Claudin localization in cilia of the retinal pigment epithelium. (8/114)

Using immunocytochemistry and confocal microscopy we demonstrate that claudin-immunoreactivity is a novel marker for retinal pigment epithelial cilia. Claudin-immunoreactivity obtained by polyclonal anti-claudin 1 antibody, which could crossreact with claudin 3, was colocalized with acetylated tubulin-immunoreactivity in cultured human retinal pigment epithelial cells. Claudin-immunoreactivity associated with the retinal pigment epithelium (RPE) cilia was more intense than was claudin-immunoreactivity in the junctional complex. Approximately two-thirds of the RPE cells in the rat contain cilia that are immunoreactive with acetylated tubulin on postnatal day 1, and a significant portion of these cilia label with the anti-claudin 1 antibody. Cilia decrease in frequency over subsequent postnatal days, and are absent by postnatal day 30. As RPE cilia decrease in number during postnatal rat development, claudin-immunoreactivity is lost earlier than acetylated tubulin, suggesting that the loss of claudin may initiate RPE cilium degeneration. Claudin-immunoreactivity was not evident in cilia of photoreceptor cells, epithelia of nasal mucosa, small intestine, or colon, suggesting that claudin may be a unique molecule in RPE cilia. These data suggest that cilia of the RPE, unlike cilia on other cell types, contain claudin, and that this molecule may play an important and specific role in the function and/or maintenance of RPE cilia.  (+info)

Claudins are major integral membrane proteins of tight junctions. Altered expression of several claudin proteins, in particular claudin-1, -3, -4 and -7, has been linked to the development of various cancers. Although their dysregulation in cancer suggests that claudins play a role in tumorigenesis, the exact underlying mechanism remains unclear. The involvement of claudins in tumor progression was suggested by their important role in the migration, invasion and metastasis of cancer cells in a tissue-dependent manner. Recent studies have shown that they play a role in epithelial to mesenchymal transition (EMT), the formation of cancer stem cells or tumor-initiating cells (CSCs/TICs), and chemoresistance, suggesting that claudins are promising targets for the treatment of chemoresistant and recurrent tumors. A recently identified claudin-low breast cancer subtype that is characterized by the enrichment of EMT and stem cell-like features is significantly associated with disease recurrence, underscoring
View mouse Cldn15 Chr5:136966616-136975858 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
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Development of innovative, effective therapies against recurrent/chemotherapy-resistant ovarian cancer remains a high priority. Using high-throughput technologies to analyze genetic fingerprints of ovarian cancer, we have discovered extremely high expression of the genes encoding the proteins claudin-3 and claudin-4. Because claudin-3 and -4 are the epithelial receptors for Clostridium perfringens enterotoxin (CPE), and are sufficient to mediate CPE binding, in this study we evaluated the in vitro and in vivo bioactivity of the carboxy-terminal fragment of CPE (i.e., CPE290-319 binding peptide) as a carrier for tumor imaging agents and intracellular delivery of therapeutic drugs. Claudin-3 and -4 expression was examined with rt-PCR and flow cytometry in multiple primary ovarian carcinoma cell lines. Cell binding assays were used to assess the accuracy and specificity of the CPE peptide in vitro against primary chemotherapy-resistant ovarian carcinoma cell lines. Confocal microscopy and biodistribution
The entire human body and its many compartments are shielded from their external environments by the barrier function of epithelial cell sheets. The paracellular barrier function of tight junctions (TJs) is critical for maintaining homeostasis in any multicellular organism, especially in the skin and internal organs and at the blood-brain barrier. One of the major components of TJs is a family of adhesive membrane proteins known as claudins. Several members of the claudin family are receptors for the bacterial toxin Clostridium perfringens enterotoxin. This toxin often causes food-borne illness both in humans and animals. Saitoh et al. crystallized a complex between the toxin and a claudin that reveals just how the toxin damages epithelial barriers (see the Perspective by Artursson and Knight).. Science, this issue p. 775; see also p. 716 ...
In this study, phosphate-rich supernatant at the end of anaerobic phase was extracted by a certain side-stream ratio for chemical precipitation to investigate the optimal conditions for phosphorus recovery. The effect of side-stream reaction on the performance of the mainstream Enhanced Biological Phosphorus Removal (EBPR) system was also explored. The experiment was carried out in a sequencing batch reactor (SBR) operated in an alternating anaerobic/aerobic mode with dissolved oxygen controlled at 1.0 mg · L−1. The results showed that the optimum magnesium source,temperature, stirring speed and reaction equilibrium time for side-stream phosphorus recovery were: MgCl2 · 6H2O, 25 °C, 150 rpm and 20 min, respectively. It was also observed that the average phosphorus removal efficiency of the mainstream system maintained as high as 90.7% during the side-stream extraction period despite insufficient time for phosphate uptake under limited dissolved oxygen condition and phosphate deprivation of ...
The ability to invade host tissues and metastasize is the major cause of cancer-related death. During tumor invasion, metastasizing cells disrupt normal cell-matrix adhesion and acquire an invasive phenotype. Claudins are adhesion proteins localized at tight junctions (TJs). Claudin-7 is a unique TJ membrane protein in that it has a stronger basolateral membrane distribution than that of apical TJs in epithelial cells. To study the basolateral function of claudin-7, claudin-7 gene silencing experiments were carried out in a lung cancer cell line using the lentivirus shRNA approach. We found that claudin-7 knockdown (KD) cells showed disrupted cell-matrix interactions. Consequently, when claudin-7 KD cells were plated on the uncoated glass surface, they were unable to attach to the glass and died the day after plating. In contrast, control cells adhered well and grew normally. Using immunofluorescent microscopy and biochemistry methods, we found that claudin-7 co-localized and ...
A side-stream respiration monitoring sensor includes a body having a first end, a second end, and a detecting section disposed between the respective ends. The detecting section includes a first port and a second port positioned on generally opposite sides of a restricting member. The restricting member extends into a flow path formed through the body such that a pressure differential is generated between the first port and the second port. A sampling port is positioned downstream relative to a patient from the first and the second port and configured to acquire a respiration sample. The sensor is constructed to monitor respiration performance of premature infants
GGRNA , 2020-04-07 23:23:15 , RefSeq release 60 (20130726)] RefSeq ID Version Symbol GeneID Definition NM_001101389 NM_001101389.1 CLDN25 644672 Homo sapiens claudin 25 (CLDN25), mRNA. NM_001111319 NM_001111319.1 CLDN22 53842 Homo sapiens claudin 22 (CLDN22), mRNA. NM_020982 NM_020982.3 CLDN9 9080 Homo sapiens claudin 9 (CLDN9), mRNA. NM_001001346 NM_001001346.3 CLDN20 49861 Homo sapiens claudin 20 (CLDN20), mRNA. NM_001306 NM_001306.3 CLDN3 1365 Homo sapiens claudin 3 (CLDN3), mRNA. NM_012131 NM_012131.2 CLDN17 26285 Homo sapiens claudin 17 (CLDN17), mRNA. NM_016369 NM_016369.3 CLDN18 51208 Homo sapiens claudin 18 (CLDN18), transcript variant 1, mRNA. NM_001185056 NM_001185056.1 CLDN11 5010 Homo sapiens claudin 11 (CLDN11), transcript variant 2, mRNA. NM_005602 NM_005602.5 CLDN11 5010 Homo sapiens claudin 11 (CLDN11), transcript variant 1, mRNA. NM_182848 NM_182848.3 CLDN10 9071 Homo sapiens claudin 10 (CLDN10), transcript variant a, mRNA. NM_006984 NM_006984.4 CLDN10 9071 Homo sapiens claudin ...
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We investigated some immunogenic properties of Clostridium perfringens enterotoxin (CPE) in vitro using murine J774A macrophages (MPhi) and in vivo using Swiss Webster (SW) mice. CPE was a potent mitogen in vitro, where cell proliferation increased with CPE concentration. CPE was nonmitogenic when M …
Claudin-4 (Clostridium perfringens enterotoxin receptor) is a tight junction protein encoded by the gene CLDN4. Expression of Claudin-4 has been associated with either poor prognosis or a more favorable diagnosis, depending on the type of cancer. Claudin-4 has been shown to distinguish adenocarcinoma from malignant mesothelioma with 99% specificity in malignant effusions (1). Claudin-4 overexpression was able to independently predict survival in a breast cancer multivariate analysis as it was associated with poor prognosis, high tumor grade and Her2 expression and was inversely correlated with estrogen receptor staining (2). In luminal breast cancer, the increase of Claudin-4 protein was correlated with the increase of tumor grade and with Ki-67, and thus demonstrated an overall shorter life survival (3). Basal-like tumors also demonstrated overexpression of Claudin-4 (4). Counter to the above breast cancer subtypes, the presence of Claudin-4 in triple negative breast cancer was a biomarker that
As mentioned in our introduction, the availability of CPE-directed therapeutics could be helpful for ameliorating several CPE-associated medical conditions. A previous study had suggested that the drug mepacrine might be a candidate CPE therapeutic because the presence of this drug interferes with CPE-induced electrophysiologic activity in artificial lipid bilayers (24). However, that study did not distinguish whether mepacrine inactivates the CPE protein or instead interferes with some step in CPE action, i.e., whether this drug affects CPE binding, CPE pore formation, or CPE pore activity. Furthermore, it was specifically important to determine whether mepacrine is not only protective against CPE electrophysiologic activity in artificial membranes but also inhibits CPE-induced cytotoxicity in mammalian cells, where receptors are present and complex phenomena like membrane vesicle release occur (30).. Therefore, a first major contribution of the current study entailed demonstrating that ...
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Read Claudin-8 Expression in Renal Epithelial Cells Augments the Paracellular Barrier by Replacing Endogenous Claudin-2, The Journal of Membrane Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
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The expression of claudin-11 in benign and malignant bladder tissue and the effect of forced expression of claudin-11 on tight junction function and invasiveness of bladder cancer cells were studied. Claudin-11 expression was tested in bladder cancer cell lines (T24/83, RT 112/84 and EJ138) using reverse transcription-polymerase chain reaction (RT-PCR) and in benign and malignant bladder tissue by quantitative RT-PCR and immunohistochemistry. T24/83 cells were transfected with the pcDNA.1/NT-GFP-TOPO vector containing full-length human claudin-11 sequence. Stable-transfected cells overexpressing claudin-11 (T24Cl-11Ex), wild-type cells (T24WT) and the empty plasmid control clone (T24GFP) were compared using transurothelial resistance (TUR), in vitro adhesion, invasion and growth assays. Claudin-11 was strongly expressed in the non-invasive RT112/84 cell line compared to the invasive T24/83 and EJ138 TCC cell lines. Benign bladder tissue demonstrated equal expression of claudin-11 mRNA as ...
The protein encoded by this intronless gene belongs to the claudin family. Claudins are integral membrane proteins that are components of the epithelial cell tight junctions, which regulate movement of solutes and ions through the paracellular space. This protein is a high-affinity receptor for Clostridium perfringens enterotoxin (CPE) and may play a role in internal organ development and function during pre- and postnatal life. This gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. [provided by RefSeq, Sep 2013 ...
The tight junction protein claudin-1 (CLDN1) has been shown to be essential for hepatitis C virus (HCV) entry-the first step of viral infection. Due to the lack of neutralizing anti-CLDN1 antibodies, the role of CLDN1 in the viral entry process is poorly understood. In this study, we produced antibodies directed against the human CLDN1 extracellular loops by genetic immunization and used these antibodies to investigate the mechanistic role of CLDN1 for HCV entry in an infectious HCV cell culture system and human hepatocytes. Antibodies specific for cell surface-expressed CLDN1 specifically inhibit HCV infection in a dose-dependent manner. Antibodies specific for CLDN1, scavenger receptor B1, and CD81 show an additive neutralizing capacity compared with either agent used alone. Kinetic studies with anti-CLDN1 and anti-CD81 antibodies demonstrate that HCV interactions with both entry factors occur at a similar time in the internalization process. Anti-CLDN1 antibodies inhibit the binding of ...
Claudin-1 is an integral membrane protein component of tight junctions. The Snail family of transcription factors are repressors that play a central role in the epithelial-mesenchymal transition, a process that occurs during cancer progression. Snail and Slug members are direct repressors of E-cadherin and act by binding to the specific E-boxes of its proximal promoter. In the present study, we demonstrate that overexpression of Slug or Snail causes a decrease in transepithelial electrical resistance. Overexpression of Slug and Snail in MDCK (Madin-Darby canine kidney) cells down-regulated Claudin-1 at protein and mRNA levels. In addition, Snail and Slug are able to effectively repress human Claudin-1-driven reporter gene constructs containing the wild-type promoter sequence, but not those with mutations in two proximal E-box elements. We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin-1 promoter. Moreover, an inverse correlation in ...
The aim of this project is to find new treatments for the most common type of ovarian cancer, high-grade serous ovarian cancer. To achieve this aim…
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudins are small (20-27 kilodalton (kDa)) transmembrane proteins which are found in many organisms, ranging from nematodes to human beings, and are very similar in their structure, although this conservation is not observed on the genetic level. Claudins span the cellular membrane 4 times, with the N-terminal end and the C-terminal end both located in the cytoplasm, and two extracellular loops which show the highest degree of conservation. The first extracellular loop consists on average of 53 amino acids and the second one, being slightly smaller, of 24 amino acids. The N-terminal end is usually very short (4-10 amino acids), the C-terminal end varies in length ...
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This study examines the expression of Mab CC188 binding epitope in ovarian cancer stem cells and their differentiated progeny in vitro and explores the feasibility of imaging ovarian xenograft tumors with Mab CC188 labeled with NIR dye using an IRDye 800CW Protein Labeling Kit from LI-COR Biosciences.
Novogen Ltd. surged as much as 263% to $7.60 in regular trading on Tuesday after the company announced that its lead experimental drug, CS-6, has proven to be highly active against ovarian cancer stem cells in laboratory studies.
The C\terminal fragment of enterotoxin (C\CPE) modulates the tight junction protein claudin and disturbs the tight junctional barrier. epithelial cells (HPDEs) had been treated with C\CPE 194 and C\CPE meters19. In well\differentiated cells of the pancreatic malignancy cell collection HPAC, C\CPE 194 and C\CPE meters19 interrupted both the hurdle and fencing features without adjustments in manifestation of claudin\1 and \4, collectively with an boost of MAPK phosphorylation. C\CPE 194, but not really C\CPE meters19, improved the cytotoxicity of the anticancer brokers gemcitabine and H\1. In differentiated pancreatic malignancy cell collection PANC\1 badly, C\CPE 194, but not really C\CPE meters19, reduced claudin\4 phrase and improved MAPK activity and the cytotoxicity of the anticancer agencies. In regular HPDEs, C\CPE 194 and C\CPE meters19 reduced claudin\4 phrase and improved the MAPK activity, whereas they do not really influence the cytotoxicity of the anticancer agencies. Our results ...
For every experimental group, brains from at minimum 3 distinct litter had been analyzed and when compared to the in accordance NaCl handle group. qPCR approach improvement exposed that only samples must be when compared to every other which have gone through experimental treatment, mind isolation, storage, purification and evaluation preparing steps with each other. Therefore, for every DEX-treatment the according NaCl handle group was carried out at the exact same time. In addition, owing to the large complete variety of samples, but limited sample variety which could be purified at the same time, only samples from mice at the same age and identical variety of antenatal injections ended up compared to every other by using a two-tailed Student`s t-take a look at. Data are offered as the signifies ± SEM. The major tight junction molecule and mind endothelial mobile marker claudin-five was investigated originally. Triple maternal DEX remedy drastically decreased claudin-5 mRNA expression to .54 ...
A combined imaging and phototherapy technique could help guide surgeons removing chemotherapy-resistant tumors and kill any cancer cells the surgeons
Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. Chromatin immunoprecipitation (ChIP) and luciferase reporter assay showed that SCF/c-kit
DESCRIPTION (provided by applicant): Disruption of the cell-cell junction with concomitant changes in the expression of junctional proteins is a hallmark of cancer metastasis and invasion. Role of adherent junction proteins have been studied extensively in cancer, however the role of tight junction proteins is less understood. Claudins are the recently identified tetraspanins, which are integral to the structure and function of tight junctions (TJs). Recent studies have shown changes in expression/cellular localization for claudins during tumorigenesis, however a cause and effect relationship has not been established. Here, we report a highly increased expression for claudin-1 in human primary colon carcinoma and metastatic tissues and cell lines derived from similar sources with relatively frequent nuclear localization. Furthermore, using genetic manipulations of claudin-1 expression in colon cancer cell lines, we demonstrate a role for claudin-1 in the regulation of epithelial to mesenchymal ...
Recent investigations revealed that epithelial TJs contain charge- and size-selective pores, which control the paracellular flux of charged and uncharged solutes. The paracellular flux occurs through two distinct pathways: one high-capacity pathway with size-restrictive pores and one low-capacity pathway that is size independent, at least for substances with radii of up to 7 Å (Van Itallie et al., 2008; Watson et al., 2001). The physical basis of the low-capacity pathway is not yet completely understood, whereas the high-capacity pathway is well described. It consists of small pores with radii of ~4 Å, and is responsible for the flux of small charged and uncharged solutes. Expression of claudin-2 affects only the high-capacity pathway, by inducing an increase in pore number and a change in charge selectivity (Van Itallie et al., 2008). In a recent attempt to model the claudin-2-induced channel (Yu et al., 2009), the pore diameter, calculated from permeability to organic cations and from the ...
Objective Helicobacter pylori strains that express the oncoprotein CagA augment risk for gastric cancer. However, the precise mechanisms through which cag+ strains heighten cancer risk have not been fully delineated and model systems that recapitulate the gastric niche are critical for understanding pathogenesis. Gastroids are three-dimensional organ-like structures that provide unique opportunities to study host-H. pylori interactions in a preclinical model. We used gastroids to inform and direct in vitro studies to define mechanisms through which H. pylori modulates expression of the cancer-associated tight junction protein claudin-7.. ...
Epithelial barrier dysfunction is a significant factor in many allergic diseases, including eosinophilic esophagitis (EoE). Infiltrating leukocytes and tissue adaptations increase metabolic demands and decrease oxygen availability at barrier surfaces. Understanding of how these processes impact barrier is limited, particularly in allergy. Here, we identified a regulatory axis whereby the oxygen-sensing transcription factor HIF-1α orchestrated epithelial barrier integrity, selectively controlling tight junction CLDN1 (claudin-1). Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial barrier function, as documented in 3D organotypic epithelial cultures. L2-IL5OXA mice with EoE-relevant allergic inflammation displayed localized eosinophil oxygen metabolism, tissue hypoxia, and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of esophageal epithelial-targeted stabilized ...
The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.
immune Uncategorized PA-824, Rabbit Polyclonal to C-RAF (phospho-Thr269). The blood-epididymis barrier (BEB) is formed by epithelial tight junctions mediating selective permeability from the PA-824 epididymal epithelium. the paracellular permeability had been examined by two strategies TER and FITC-Dextran-based tracer diffusion assays. Both assays soon add up to related outcomes indicating a time-dependent disruption from the BEB differentially for the three TGF? isoforms (TGF?3>TGF?1>TGF?2) inside a TGF?-recetor-1 kinase- and Smad-dependent way. The small junction proteins claudin-1 was discovered to be decreased by the procedure with TGF?s whereas occludin had not PA-824 been affected. Epididymal epithelial cells are mainly attentive to TGF?s PA-824 through the basolateral side recommending that TGF? may impact for the epididymal epithelium through the stroma cell tradition versions the knockdown of 1 of the claudins (1 -3 -4 or -7) led to dramatically reduced transepithelial electrical level ...
Vidal, Christian M. et al Abstract C271: FAK inhibitor defactinib (VS-6063) enhances the efficacy of paclitaxel and preferentially targets ovarian cancer stem cells.. Molecular Cancer Therapeutics 12.11 Supplement (2013): C271. Web. 07 Aug. 2020. ...
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Sigma-Aldrich offers abstracts and full-text articles by [S K Tiwari-Woodruff, A G Buznikov, T Q Vu, P E Micevych, K Chen, H I Kornblum, J M Bronstein].
Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans cells in the epidermis. Our objective was to identify immunoreactive CLDN1 in human epidermal Langerhans cells and to examine the pattern of epidermal Langerhans cells in genetic human CLDN1 deficiency [neonatal ichthyosis, sclerosing cholangitis (NISCH) syndrome]. Epidermal cells from healthy human skin labelled with CLDN1-specific antibodies were analysed by confocal laser immunofluorescence microscopy and flow cytometry. Skin biopsy sections of two patients with NISCH syndrome were stained with an antibody to CD1a expressed on epidermal Langerhans cells. Epidermal Langerhans cells and a subpopulation of keratinocytes from healthy skin were positive for CLDN1. The gross number and distribution of epidermal Langerhans cells of two patients with molecularly confirmed NISCH syndrome, however, was not grossly altered. Therefore, CLDN1 is unlikely to play a critical role in ...
HCV is a leading cause of hepatocellular carcinoma and cirrhosis all over the world. Claudins belong to family of tight junctions proteins that are responsible for establishing barriers for controlling the flow of molecules around cells. For therapeutic strategies, regulation of viral entry into the host cells holds a lot of promise. During HCV infection claudin-1 is highly expressed in liver and believed to be associated with HCV virus entry after HCV binding with or without co-receptor CD81. The claudin-1 assembly with tight junctions is regulated by post translational modifications. During claudins assembly and disassembly with tight junctions, phosphorylation is required at C-terminal tail. In cellular proteins, interplay between phosphorylation and O-β-GlcNAc modification is believed to be functional switch, but it is very difficult to monitor these functional and vibrant changes in vivo. Netphos 2.0 and Disphos 1.3 programs were used for potential phosphorylation; NetPhosK 1.0 and KinasePhos for
Am J Reprod Immunol. 2011 Apr;65(4):438-47. doi: 10.1111/j.1600-0897.2010.00914.x. Epub 2010 Sep 6. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Govt
From NCBI Gene:. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. It is found primarily in the kidneys, specifically in the thick ascending limb of Henle, where it acts as either an intercellular pore or ion concentration sensor to regulate the paracellular resorption of magnesium ions. Defects in this gene are a cause of primary hypomagnesemia, which is characterized by massive renal magnesium wasting with hypomagnesemia and hypercalciuria, resulting in ...
This gene encodes an integral membrane protein, which belongs to the claudin family. The protein is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. This gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems ...
Nestlé has unveiled a new chocolate recipe that replaces refined sugar with cocoa pulp as it begins to explore the possibilities of side-stream utilisation to reduce food waste.
Cytoplasmic expression of claudin-1 in metastatic melanoma cells correlates to increased migration, and increased secretion of MMP-2 in a PKC dependent manner, whereas claudin-1 nuclear expressi...
Complete information for CLDN18 gene (Protein Coding), Claudin 18, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human Gene Compendium
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Familial hypomagnesemia with hypercalciuria and nephrocalcinosis is an autosomal recessive tubular disorder characterized by excessive renal magnesium and calcium excretion and chronic kidney failure. This rare disease is caused by mutations in the CLDN16 and CLDN19 genes. These genes encode the tight junction proteins claudin-16 and claudin-19, respectively, which regulate the paracellular ion reabsortion in the kidney. Patients with mutations in the CLDN19 gene also present severe visual impairment. Our goals in this study were to examine the clinical characteristics of a large cohort of Spanish patients with this disorder and to identify the disease causing mutations. We included a total of 31 patients belonging to 27 unrelated families and studied renal and ocular manifestations. We then analyzed by direct DNA sequencing the coding regions of CLDN16 and CLDN19 genes in these patients. Bioinformatic tools were used to predict the consequences of mutations. Clinical evaluation showed ocular defects in
Tight junctions between epithelial and endothelial cells form selective barriers and paracellular channels and regulate paracellular transport of solutes, immune cells, and drugs. More specifically, tight junctions consist of proteins that laterally interconnect neighboring cells of epithelia and endothelia. Certain proteins seal the tight junction, so that a nearly impermeable barrier develops, whereas others form channels that allow for permeation between the cells. Recent investigations have focused on tight junction proteins, belonging to the claudin family (claudins-1 to -27 in humans) and the newly defined group of TAMP (three proteins: occludin, Marvel-D2, and tricellulin). Barriers and Channels Formed by Tight Junction Proteins I showcases work in this area clustered around three major themes: the molecular properties of tight junctions, for example, the role of the claudin family of proteins and the formation of ion and charge-selective channels; the regulation of tight junction
Crohns disease; infective colitis is often a cause of one episode of colitis which is mislabelled as ulcerative colitis e.g. transverse colon the portion Causes and symptoms. Biocare Probiotics 30 Billion Which Uk Are Best benefits of Digestive Enzymes for Cats. John McDougall in his book The McDougall Plan summarizes the multiple benefits of fiber (some info Ive taken from other sources too): Fiber has no calories since Probiotic dosages are listed Introduction Giardia Clostridium perfringens enterotoxin and Cryptosporidium are important causes of diarrhea in dogs and cats. Find and study online flashcards from Pathophysiology 3400. Colorectal cancer commonly known as colon cancer or bowel cancer is a melanoma from uncontrolled cell growth in the Probiotics can also play Biocare Probiotics 30 Billion Which Uk Are Best a role in maintaining oral health (bad eath). I know there have been some posts dealing with probiotics and many have questions about wh Stage IIB: Cancer has C colon ...
TABLE-US-00004 TABLE 4 Cell and biological adhesion Fold Symbol Gene name Gene assignment change CLDN7 claudin 7 Involved in the formation of 2.74 tight junctions between epithelial cells PCDHB5 Protocadherin Member of the 3.32 beta-5 protocadherin beta gene cluster CLDN3 Claudin 3 Member of the claudin 3.93 family, is an integral membrane protein and a component of tight junction strands. CNTN6 contactin 6 Contactins mediate cell 3.09 surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. PKHD1 polycystic kidney Localized predominantly at 3.38 and hepatic the disease 1 apical domain of polarized epithelial cells, suggesting it may be involved in the tubulogenesis and/or maintenance of duct-lumen architecture. PCDHB2 protocadherin The extracellular domains 2.94 beta 2 interact in a homophilic manner to specify differential cell-cell connections. CDH1 E-cadherin cell adhesion molecule 3.27 (epithelial) CX3CL1 hemokine ...
Claudin 7 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Enzyme-linked Immunosorbent Assay (ELISA) applications. This antibody reacts with human,mouse samples. Cat.No. 10118-1-AP.
Claudin, Philippe; Andreotti, Bruno (2006). "A scaling law for aeolian dunes on Mars, Venus, Earth, and for subaqueous ripples ... 58 (2-3): 323-336. doi:10.7202/013146ar. ISSN 1492-143X. Silvestro, S.; Chojnacki, M.; Vaz, D. A.; Cardinale, M.; Yizhaq, H.; ... 19 (3-4): 173-210. doi:10.1111/j.1365-3091.1972.tb00020.x. ISSN 0037-0746. Cooper, William S. (1958), "COASTAL SAND DUNES OF ... 2177-2185, doi:10.1007/978-1-4614-3134-3_380, ISBN 978-1-4614-3134-3, retrieved 2020-09-16 Vriend, N. M.; Jarvis, P. A. (2018 ...
Bordiec, M.; Carpy, S.; Bourgeois, O.; Herny, C.; Massé, M.; Perret, L.; Claudin, P.; Pochat, S.; Douté, S. (1 December 2020 ... 2020, p. 3. Bintanja 1999, p. 346. Bintanja 1999, p. 347. Bintanja 1999, p. 344. Bintanja 1999, p. 355. Harvey 2003, p. 102. ... This sublimation occurs at rates of 3-350 centimetres per year (1.2-137.8 in/year) snow water equivalent and is balanced by ice ...
Ernstson, K.; Claudin, F.; Schüssler, U.; Hradil, K. (2002). "The mid-Tertiary Azuara and Rubielos de la Cérida paired impact ... 3, pp. 285-307 Zerelia East & West Dietrich, V. J; Lagios, E; Reusser, E; Sakkas, V; Gartzos, E; Kyriakopoulos, K (2013). "The ... ISBN 978-3-319-13092-7. Chabou, M. C. (2016). "An updated inventory of meteorite impact structures in the Arab world". 1st ... ISBN 978-3-319-13093-4. Maximiliano C.L. Rocca et al. (2017). (2017). "Geophysical evidence for a large impact structure on the ...
ISBN 978-84-7222-878-8. Azcárate, Manuel; Fernando Claudin (1979). Marc Abeles; Charles-Albert Ryng (eds.). L'Europe de ... ISBN 978-84-336-0193-3. Azcárate, Manuel (1982). preface. Le Mouvement communiste à la croisée des chemins. By Schaff, Adam (in ... ISBN 2-7071-1102-3. Azcárate, Manuel (1980). Interrogantes ante la izquierda (in Spanish). Marc Abeles, Charles-Albert Ryng, ...
Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. It is a member of the claudin ... "Entrez Gene: CLDN3 claudin 3". Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG (Nov 2003). "Role of claudin ... The protein encoded by this intron-less gene, a member of the claudin family, is an integral membrane protein and a component ... Overview of all the structural information available in the PDB for UniProt: Q9Z0G9 (Mouse Claudin-3) at the PDBe-KB. Kniesel U ...
Claudin-19 is a protein that in humans is encoded by the CLDN19 gene. It belongs to the group of claudins. Claudin-19 has been ... "Entrez Gene: CLDN19 claudin 19". Naeem, M.; Hussain, S.; Akhtar, N. (2011). "Mutation in the Tight-Junction Gene Claudin 19 ( ... 2006). "Kidney claudin-19: localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease". ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-11 is a protein that in humans is encoded by the CLDN11 gene. It belongs to the group of claudins and was the first ... "Entrez Gene: CLDN11 claudin 11 (oligodendrocyte transmembrane protein)". Gow A, Southwood CM, Li JS, Pariali M, Riordan GP, ... The protein encoded by this gene belongs to the claudin family of tight junction associated proteins and is a major component ... Tiwari-Woodruff SK, Buznikov AG, Vu TQ, Micevych PE, Chen K, Kornblum HI, Bronstein JM (April 2001). "OSP/claudin-11 forms a ...
Claudin domain-containing protein 1 is a protein that in humans is encoded by the CLDND1 gene. GRCh38: Ensembl release 89: ... "Entrez Gene: CLDND1 claudin domain containing 1". Human CLDND1 genome location and CLDND1 gene details page in the UCSC Genome ... 56 (3): 307-17. doi:10.1016/j.lungcan.2007.01.016. PMID 17316888. Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence ... doi:10.1016/S0378-1119(02)00507-3. PMID 12036590. Zhang QH, Ye M, Wu XY, et al. (2001). "Cloning and Functional Analysis of ...
Claudin, F., Ernstson, K., Rampino, M.R., and Anguita, F. 2001. Striae, polish, imprints, rotated fractures, and related ... Ernstson, K., Claudin, F., Schüssler, U., Anguita, F. and Ernstson, T. 2001. Impact melt rocks, shock metamorphism, and ... Missing or empty ,title= (help) Ernstson, K., Claudin, F., Schüssler, U. & Hradil, K. (2002): The mid-Tertiary Azuara and ... 1], Ernstson, K., Schüssler, U., Claudin, F., and Ernstson, T. (2003). An impact crater chain in northern Spain. Meteorite, 9, ...
Missing or empty ,title= (help) A. Claudin, The first Paris press; an account of the books printed for G. Fichet and J. Heynlin ... ISBN 3-88309-035-2. (in German) Information und Kommunikation in Geschichte und Gegenwart This article incorporates text from a ...
Beeman N, Webb PG, Baumgartner HK (February 2012). "Occludin is required for apoptosis when claudin-claudin interactions are ... 73 (3): 222-31. PMID 9243183. Haskins J, Gu L, Wittchen ES, Hibbard J, Stevenson BR (April 1998). "ZO-3, a novel member of the ... 3 (2): e273. doi:10.1038/cddis.2012.14. PMC 3288343. PMID 22361748. Osanai M, Murata M, Nishikiori N, Chiba H, Kojima T, Sawada ... 30 (3): 1234-46. doi:10.1096/fj.15-277673. PMC 4750406. PMID 26601824. Furuse M, Hirase T, Itoh M, Nagafuchi A, Yonemura S, ...
Claudin 4, also known as CLDN4, is a protein which in humans is encoded by the CLDN4 gene. It belongs to the group of claudins ... "Entrez Gene: CLDN4 claudin 4". Human CLDN4 genome location and CLDN4 gene details page in the UCSC Genome Browser. Kniesel U, ... This gene encodes an integral membrane protein, which belongs to the claudin family. The protein is a component of tight ... 2003). "Claudin-4 expression decreases invasiveness and metastatic potential of pancreatic cancer". Cancer Res. 63 (19): 6265- ...
Claudin de Sermisy, composer (born c.1490) 31 October - Augustin Marlorat. Protestant reformer (born 1506) 19 December - ... 1915). "Biron, Charles de Gontaut". Salmonsens Konversationsleksikon (in Danish). 3 (2 ed.). Copenhagen: J. H. Schultz ...
Claudin-5 is a protein that in humans is encoded by the CLDN5 gene. It belongs to the group of claudins. This gene encodes a ... "Entrez Gene: CLDN5 claudin 5 (transmembrane protein deleted in velocardiofacial syndrome)". Coyne CB, Gambling TM, Boucher RC, ... Kojima S, Rahner C, Peng S, Rizzolo LJ (2002). "Claudin 5 is transiently expressed during the development of the retinal ... Tsukita S, Furuse M (2002). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. It belongs to the group of claudins. Claudins, such as ... "Entrez Gene: CLDN7 claudin 7". CS1 maint: discouraged parameter (link) Human CLDN7 genome location and CLDN7 gene details page ... 2005). "Claudin-1 is a strong prognostic indicator in stage II colonic cancer: a tissue microarray study". Mod. Pathol. 18 (4 ... 2003). "Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and ...
The WT mice were shown to have lower claudin-5 and occludin levels than the KO mice after TBI. Claudin and occludin are ... Furuse M, Fujita K, Hiiragi T, Fujimoto K, Tsukita S (Jun 1998). "Claudin-1 and -2: novel integral membrane proteins localizing ... Both the WT and KO tissues showed a drop in claudin-5, occludin, and laminin-α1 (a basal lamina protein), suggesting that MMP-3 ... "Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice". The Journal of Cell Biology. 161 (3): 653-60 ...
"Entrez Gene: CLDN1 claudin 1". Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG (Nov 2003). "Role of claudin ... Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. It belongs to the group of claudins. Tight junctions ... The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight ... Miyamori H, Takino T, Kobayashi Y, Tokai H, Itoh Y, Seiki M, Sato H (2001). "Claudin promotes activation of pro-matrix ...
... claudin-2, claudin-6, claudin-7 and occludin) and transcription factors (including GATA4). Changes in the expression of claudin ... Paschoud S, Bongiovanni M, Pache JC, Citi S (September 2007). "Claudin-1 and claudin-5 expression patterns differentiate lung ... Guillemot L, Citi S (August 2006). "Cingulin regulates claudin-2 expression and cell proliferation through the small GTPase ... Together with paracingulin, cingulin also was reported to regulate claudin-2 expression through RhoA-dependent and independent ...
Nevertheless, another sources document existence of mTEC unipotent progenitors that express claudin 3 and 4 (Cld3/4). These two ... "Medullary thymic epithelial cells expressing Aire represent a unique lineage derived from cells expressing claudin". Nature ... 3 (5): 383-91. doi:10.1038/nri1085. PMID 12766760. Kurobe H, Liu C, Ueno T, Saito F, Ohigashi I, Seach N, Arakaki R, Hayashi Y ... 41 (3): 414-426. doi:10.1016/j.immuni.2014.08.007. PMC 4175925. PMID 25220213. Kroger CJ, Spidale NA, Wang B, Tisch R (January ...
"Medullary thymic epithelial cells expressing Aire represent a unique lineage derived from cells expressing claudin". Nature ... 3 (5): 383-391. doi:10.1038/nri1085. ISSN 1474-1733. PMID 12766760. Ueno, Tomoo; Saito, Fumi; Gray, Daniel H. D.; Kuse, Sachiyo ... 15 (3): 1101-1111. doi:10.1091/mbc.e03-09-0704. ISSN 1059-1524. PMC 363084. PMID 14699058. Feng, Yuchen; He, Ding; Yao, Zhiyuan ... 8 (3): 304-311. doi:10.1038/ni1438. ISSN 1529-2908. PMID 17277780. Sekai, Miho; Hamazaki, Yoko; Minato, Nagahiro (November 2014 ...
... claudin-7, CD44 variant isoforms, and tetraspanins promotes colorectal cancer progression". Molecular Cancer Research. 5 (6): ... 38 (3): 577-88. doi:10.1053/jhep.2003.50376. PMID 12939584. S2CID 46013740. Kuhn S, Koch M, Nübel T, Ladwein M, Antolovic D, ... doi:10.1016/S0378-1119(97)00411-3. PMID 9373149. Serru V, Le Naour F, Billard M, Azorsa DO, Lanza F, Boucheix C, Rubinstein E ( ...
This allows the human claudin-3,4,6,7,8 and 14 to bind but not 1,2,5, and 10. The way the protein work is it destroys the cell ... Van Itallie CM, Betts L, Smedley JG, McClane BA, Anderson JM (January 2008). "Structure of the claudin-binding domain of ... This mechanism is mediated by host claudin-3 and claudin-4 receptors, situated at the tight junctions. Clostridium enterotoxin ... "Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium". Cancer ...
2007). "Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus". J. Virol. 81 (22): 12465-71. doi: ... Claudin-9 is a protein that in humans is encoded by the CLDN9 gene. It belongs to the group of claudins. This gene is involved ... "Entrez Gene: CLDN9 claudin 9". Gene discovery reveals a critical protein's function in hearing Human CLDN9 genome location and ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Nelson, Kevin S.; Furuse, Mikio; Beitel, Greg J. (2010-04-20). "The Drosophila Claudin Kune-kune Is Required for Septate ... 4 (3): 225-36. doi:10.1038/nrm1055. PMID 12612641. Faivre-Sarrailh C, Banerjee S, Li J, Hortsch M, Laval M, Bhat MA (October ... 66 (3): 451-64. doi:10.1016/0092-8674(81)90009-x. PMID 1651169. Ward, Robert E.; Lamb, Rebecca S.; Fehon, Richard G. (1998-03- ... 185 (3): 831-839. doi:10.1534/genetics.110.114959. ISSN 0016-6731. PMC 2907205. PMID 20407131. Wu VM. Sinuous and varicose ...
H. Elbelrhiti; P. Claudin & B. Andreotti (2005). "Field evidence for surface-wave-induced instability of sand dunes". Nature. ... March 3, 2008). "Sand Dunes Thawing on Mars". Astronomy Picture of the Day. NASA. Nemiroff, R.; Bonnell, J., eds. (April 20, ...
Patients with ALS have been found to have decreased expression of certain proteins, like ZO-1 or claudin-5, that weaken the ... "Involvement of Claudin-11 in Disruption of Blood-Brain, -Spinal Cord, and -Arachnoid Barriers in Multiple Sclerosis." Molecular ... 159(3):576-582. Chan GN, Evans RA, Banks DB, Mesev EV, Miller DS, Cannon RE. Selective induction of P-glycoprotein at the CNS ... 41(3):60-63. doi: 10.18773/austprescr.2018.022. Epub 2018 Jun 1. PMID 29921999; PMCID: PMC6003018. Li HL, Huang Y, Zhou YL, ...
Xu H, Liu Y, He G, Rossiter SJ, Zhang S (November 2013). "Adaptive evolution of tight junction protein claudin-14 in ... 53 (3): 891-906. doi:10.1016/j.ympev.2009.08.018. PMID 19699809. Liu Y, Rossiter SJ, Han X, Cotton JA, Zhang S (October 2010 ... 21 (3): 149-56. doi:10.1016/j.tree.2006.01.001. PMID 16701491. Grinnell AD (1995). "Hearing in Bats: An Overview.". In Popper ... 15 (3): 135-198, especially pp. 165-178. doi:10.2307/1536066. JSTOR 1536066. Maxim H (7 September 1912). "The sixth sense of ...
Paris : A. Claudin, 1865 Google books. ...
Fernando Claudin 11."The First Breach: The Excommunication of Yugoslavia" - Josip Tito 12. "Secret Report to the 20th Party ... 1."Social Relations in the Soviet Union" - Leon Trotsky 2."The 'Professional Dangers' of Power - Christian Rakovsky 3. "What is ...
"Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane ... 60 (3): 265-73. doi:10.1016/0378-1097(90)90315-h. PMID 2083839. Jones GW, Nielsen-Leroux C, Yang Y, Yuan Z, Dumas VF, Monnerat ... 69 (3): 197-204. doi:10.1006/jipa.1997.4660. PMID 9170345. Opota O, Gauthier NC, Doye A, Berry C, Gounon P, Lemichez E, Pauron ... 68 (3): 1137-45. doi:10.1128/AEM.68.3.1137-1145.2002. PMC 123759. PMID 11872461. PDB 3o44De S, Olson R (May 2011). "Crystal ...
Famous composers of these "Parisian" chansons included Claudin de Sermisy and Clément Janequin. Janequin's La guerre, written ... 3] As a French citizen and historian, Michelet also claimed the Renaissance as a French movement.[4] His work is at the origin ...
Claudin 5 is transiently expressed during the development of the retinal pigment epithelium. J Membr Biol 186: 81-88, 2002 ... 2] IPM에 의하여 망막색소상피 전구체가 망막색소상피세포로 성숙된다.[3] 해부학적 위치[편집]. 망막색소상피세포는 육각형 모양의 세포들이 단일층을 구성하고 있다. [4] 이 세포들은 색소 과립 (pigmented ... 기형종(Teratoma, 테라토마) : 인체 내의 3배엽 중에서 한 가지 배엽 이상의 세포가 발견되는 암을 의미함.(출처 : [[2]]) 혹은, 3배엽(외배엽, 내배엽, 중배엽) 유래의 세포나 조직이 관찰되는 종양을 의미한다.[ ... 국내에서는 차의과대학교의 송원경 교수팀이 역시 배아줄기세포 유래의 망막색소상피세포를 이용하여 노인성 황반변성 세포 치료를 시도하여, 4명 중 3명에게서 유의미한 치료 효과가 있음을 입증하였다.[47] ...
CLDND1: Claudin domain containing 1. *CPN2: Carboxypeptidase N subunit 2. *CPOX: coproporphyrinogen oxidase (coproporphyria, ... 3. q 29. 11175. 11700. 192,600,001. 198,295,559 gneg. References[edit]. *^ "Human Genome Assembly GRCh38 - Genome Reference ... G-bands of human chromosome 3 in resolution 850 bphs[4] Chr. Arm[17] Band[18] ISCN. start[19] ISCN. stop[19] Basepair. start ... "Chromosome 3". Genetics Home Reference. Retrieved 2017-05-06.. *. "Chromosome 3". Human Genome Project Information Archive 1990 ...
Sertoliho bunky tvoria pomocou tesných spojov (proteínmi ako je claudin alebo occludin) hemato-semenníkovú bariéru, ktorá ... 3, s. 419-427. PMID: 16131605. Dostupné online [cit. 2018-08-22]. ISSN 0804-4643. DOI: 10.1530/eje.1.01982. (po anglicky). ... 1, s. 3-11. Dostupné online [cit. 2018-08-22]. ISSN 0920-1742. DOI: 10.1007/s10695-012-9606-4. (po anglicky). ... 3, s. 313 - 328. Dostupné online [cit. 2018-04-20]. ISSN 1553-0795. DOI: 10.1002/aja.1001480303. (po anglicky). ...
Claudin 1, which is a tight-junction protein, and CD81 link to create a complex, priming them for later HCV infection processes ... 17 (3): 541-7. doi:10.3851/IMP2018. PMID 22300961.. *^ Bitetto D, Fattovich G, Fabris C, Ceriani E, Falleti E, Fornasiere E, ... 3 (3): 278-87. PMID 11497352.. *^ Berry KE, Waghray S, Mortimer SA, Bai Y, Doudna JA (October 2011). "Crystal structure of the ... 5 (3): 129-51. doi:10.1089/mcg.2000.5.129. PMID 11252351.. *^ Jubin R (2001). "Hepatitis C IRES: translating translation into a ...
Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane ... vermutlich Claudin bei CPE,[14] sowie vermutlich GPI-Anker oder andere Glykosylierungen beim ε-Toxin. Durch die ... Eukaryotische MACPF-ähnliche Proteine kommen beim Perforin und dem C9 des Komplementsystems als Teil der Immunabwehr vor.[3] ... F. Gisou van der Goot, Pore-forming toxins, Springer, 2001, ISBN 3-540-41386-3. ...
88 (3): 351-9. PMID 3448099.. *^ a b Gruijters, WTM (1989). "A non-connexon protein (MIP) is involved in eye lens gap-junction ... 74 (3): 327-36. doi:10.1006/exer.2002.1155. PMID 12014914.. *^ Little, JB; Azzam, EI; De Toledo, SM; Nagasawa, H (2002). " ... 93 (3): 509-13. PMID 2691517.. *^ Robertson, JD (October 1963). "The occurrence of a subunit pattern in the unit membranes of ... 11 (3): 127-30. doi:10.1002/mc.2940110302. PMID 7945800.. *^ Colombo, Bruno M.; Benedetti, Sara; Ottolenghi, Sergio; Mora, ...
Anatole Claudin (1898). The First Paris Press: an account of the books printed for G. Fichet and J. Heynlin in the Sorbonne, ... Volumes 3-4 (2011): Books published in France before 1601 in Latin and Languages other than French ... The same five also topped the list in 2016 and 2017.[2][3] ...
These complexes, formed primarily of members of the claudin and the occludin families, consist of about 35 different proteins, ... 124 (1): 3-22. doi:10.1016/j.jaci.2009.05.038. ISSN 0091-6749. PMC 4266989. PMID 19560575. Bennett, M. V.; Barrio, L. C.; ... 6 (3): 305-320. doi:10.1016/0896-6273(91)90241-q. ISSN 0896-6273. PMID 1848077. Nekrasova, Oxana; Green, Kathleen J. (1 ...
Claudin-12 is a protein that in humans is encoded by the CLDN12 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN12 claudin 12". Human CLDN12 genome location and CLDN12 gene details page in the UCSC Genome Browser. Kniesel ... 2001). "claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor, encodes lung- and ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
... is concentrated at tight junctions through its possible interaction with claudin-1 and junctional adhesion molecule". The ... 114 (Pt 3): 539-47. PMID 11171323. Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H ... 1 (3): 287-92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614. Ebnet K, Suzuki A, Horikoshi Y, Hirose T, Meyer Zu ... 154 (3): 491-7. doi:10.1083/jcb.200103047. PMC 2196413. PMID 11489913. Hamazaki Y, Itoh M, Sasaki H, Furuse M, Tsukita S (Jan ...
... has overexpressed HER2/neu Normal breast-like Claudin-low: a more recently described class; often triple-negative, but distinct ... 8-9 Grade 3 tumor (poorly differentiated). Worst prognosis. Lower-grade tumors, with a more favorable prognosis, can be treated ... 6 (3): R149-56. doi:10.1186/bcr767. PMC 400666. PMID 15084238. Evans, A. (2004). "Ductal carcinoma in situ (DCIS): are we ... Well differentiated is given a grade of 1, moderate is grade 2, while poor or undifferentiated is given a higher grade of 3 or ...
Claudin E is a molecule found in tight junctions that appears to be expressed in the EVL and required for normal zebrafish ... "The tight junction component claudin E is required for zebra fish epiboly". Developmental Dynamics. 239 (2): 715-722. doi: ... 206 (3): 381-426. doi:10.1002/jez.1402060310. PMID 568653. L. Solnica-Krezel; W. Driever (1994-09-01). "Microtubule arrays of ... 5 (3): e1000413. doi:10.1371/journal.pgen.1000413. PMC 2652113. PMID 19282986. Solnica-Krezel L (March 2005). "Conserved ...
Markov AG, Falchuk EL, Kruglova NM, Radloff J, Amasheh S (January 2016). "Claudin expression in follicle-associated epithelium ... 142 (3): 592-601.e3. doi:10.1053/j.gastro.2011.11.039. PMID 22155637. Bonnardel J, Da Silva C, Henri S, Tamoutounour S, Chasson ... 8 (3): 180-5. doi:10.1097/00054725-200205000-00004. PMID 11979138. S2CID 22514793. Jung C, Hugot JP, Barreau F (September 2010 ...
The name claudin comes from Latin word claudere ("to close"), suggesting the barrier role of these proteins. A recent review ... All human claudins (with the exception of Claudin 12) have domains that let them bind to PDZ domains of scaffold proteins. ... "Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin". J. ... "A systems proteomics view of the endogenous human claudin protein family". J Proteome Res. 15 (2): 339-359. doi:10.1021/acs. ...
Claudin de Sermisy, Thomas Crecquillon, Domenico Ferrabosco, Jean de Latre, Jacquet de Berchem, Jakob Meiland, Alexander ... ISBN 3-7751-0440-2. "RISM entry for Ms. 300 R/Vv,123". Répertoire International des Sources Musicales. Retrieved 2 June 2016. ... 3. Musica Iagellonica. ISBN 978-83-7099-161-6. Rauschning, Hermann (1931). Geschichte der Musik und Musikpflege in Danzig. ... ISBN 3-7957-0531-2. Beckmann, Klaus (2001). Repertorium Orgelmusik 1150-2000 (in German). 1. Schott Musik International. ISBN 3 ...
Claudin, P; Andreotti, B (2006). "A scaling law for aeolian dunes on Mars, Venus, Earth, and for subaqueous ripples". Earth and ... Retrieved December 3, 2012. Yen, A.S.; Kim, S.S.; Hecht, M.H.; Frant, M.S.; Murray, B. (2000). "Evidence that the reactivity of ... Even the 2001 global dust storms on Mars moved only the equivalent of a very thin dust layer - about 3 µm thick if deposited ... The Martian atmospheric dust particles are generally 3 µm in diameter. It is important to note that while the atmosphere of ...
"Systems Proteomics View of the Endogenous Human Claudin Protein Family". Journal of Proteome Research. 15 (2): 339-359. doi: ... 3 (6): 1234-42. doi:10.1021/pr049882h. PMID 15595733. Fenyo, D.; Phinney, B. S.; Beavis, R. C. (2007). "Determining the Overall ... 15 (3): 983-990. doi:10.1021/acs.jproteome.5b01018. ISSN 1535-3893. PMID 26842767. McAfee, Alison; Harpur, Brock A.; Michaud, ...
Claudin de Sermisy, French composer (b. 1495) October 18 - Anne d'Alençon, French noblewoman (b. 1492) November 7 - Maldeo ... ISBN 978-3-8228-1699-8. Vignola. Canon of the Five Orders of Architecture, translated with an introduction by Branko Mitrovic. ... February 3 - Georg Giese, German merchant (b. 1497) May 4 - Lelio Sozzini, Italian Protestant theologian (b. 1525) July 1 - ...
"Claudin-1 overexpression effect on lung adenocarcinoma cell line". NCBI GEO Profiles. Retrieved 4 May 2015. Vandepoele K, Van ... 10 (3): e1004241. doi:10.1371/journal.pgen.1004241. PMC 3961203. PMID 24651471. Vandepoele K, Andries V, Van Roy N, Staes K, ... 91 (3): 444-54. doi:10.1016/j.ajhg.2012.07.016. PMC 3511999. PMID 22901949. "NCBI BLAST". NCBI. Retrieved 4 May 2015. Hedges SB ... 3 (5): e2207. Bibcode:2008PLoSO...3.2207V. doi:10.1371/journal.pone.0002207. PMC 2386287. PMID 18493581. Burkhard P, Stetefeld ...
Claudin de Sermisy set the psalm. In the 17th century, Henry Dumont set this psalm for La Chapelle Royale au Louvre (1666). In ... Retrieved 3 April 2018. Scherman, Rabbi Nosson (1985). The Complete Artscroll Machzor - Rosh Hashanah (1st ed.). Mesorah ... Volume 3.i. A&C Black. p. 497. ISBN 9780567604521. Scherman 2003, p. 402. Brauner, Reuven (2013). "Shimush Pesukim: ...
... is concentrated at tight junctions through its possible interaction with claudin-1 and junctional adhesion molecule". J. Biol. ... 27 (3): 390-404. doi:10.2164/jandrol.05166. PMID 16452527. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M ( ... J. 361 (Pt 3): 525-36. doi:10.1042/0264-6021:3610525. PMC 1222335. PMID 11802782. Ullmer C, Schmuck K, Figge A, Lübbert H (1998 ... J. 361 (Pt 3): 525-36. doi:10.1042/0264-6021:3610525. PMC 1222335. PMID 11802782. Poliak S, Matlis S, Ullmer C, Scherer SS, ...
Plus CAPOX Compared With Placebo Plus CAPOX as First-line Treatment of Subjects With Claudin (CLDN) 18.2-Positive, HER2- ... June 3, 2016), "Der große Schlag im Kampf gegen den Krebs", WirtschaftsWoche (in German) (23), p. 14 "BioNTech-Aktie", finanzen ... 95 Prozent Wirksamkeit nach Phase-3-Auswertung (in German), retrieved November 24, 2020 Gelles, David (November 10, 2020). "The ...
Van Itallie CM, Mitic LL, Anderson JM (July 2012). "SUMOylation of claudin-2". Annals of the New York Academy of Sciences. 1258 ... 2 (3): e23931. doi:10.4161/jkst.23931. PMC 3772101. PMID 24069549. Morales JK, Falanga YT, Depcrynski A, Fernando J, Ryan JJ ( ... 25 (3): 112-4. doi:10.1016/s0968-0004(99)01537-6. PMID 10694879. Glass CK, Rosenfeld MG (January 2000). "The coregulator ... 7 (3): e32777. Bibcode:2012PLoSO...732777L. doi:10.1371/journal.pone.0032777. PMC 3296744. PMID 22412924. "FDA Press Release ...
"Tradition and Imitation in Pierre Certon's Déploration for Claudin de Sermisy". Revue de Musicologie. 85 (1): 29-62. doi: ... 85 (3): 567-591. doi:10.1017/S0038713410001302. JSTOR 27866936. S2CID 162617845. Magnan, Robert (1993). "Eustache Deschamps and ...
... expression is significantly increased after the overexpression of claudin-1 in lung adenocarcinoma cell lines. Claudin ... C11orf49 expression is significantly increased after the overexpression of Claudin-1 in lung adenocarcinoma cells. C11orf49 ... Table 3. Predicted phosphorylation sites for the C11orf49 human protein. The C11orf49 protein is predicted to be sumoylated at ... This results in a distinct N-terminus and a missing internal region near the 3' terminus compared to variant 2. Variants 6 and ...
IPR006187 Claudin. IPR003549 Claudin3. IPR017974 Claudin_CS. IPR004031 PMP22/EMP/MP20/Claudin. ... IPR006187 Claudin. IPR003549 Claudin3. IPR017974 Claudin_CS. IPR004031 PMP22/EMP/MP20/Claudin. ... Belongs to the claudin family.Curated. Keywords - Domaini. Transmembrane, Transmembrane helix. Phylogenomic databases. ... sp,O15551,CLD3_HUMAN Claudin-3 OS=Homo sapiens OX=9606 GN=CLDN3 PE=1 SV=1 ...
The lollipop plot above illustrates recurrent (observed in 3 or more out of 4440 TCGA tumor samples from 15 cancer types) and ...
CLDN3 (claudin 3). NCBI. Ortholog. Mus musculus (house mouse):. Cldn3 (claudin 3). Transitive Ortholog Pipeline. Transitive ... Cldn3 (claudin 3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Sus scrofa (pig):. CLDN3 (claudin 3). Transitive ... Cldn3 (claudin 3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Canis lupus familiaris (dog):. CLDN3 (claudin 3) ... CLDN3 (claudin 3). Transitive Ortholog Pipeline. Transitive Ortholog Pipeline. Heterocephalus glaber (naked mole-rat):. Cldn3 ( ...
Claudin-3 (CLDN3) is a ubiquitously expressed TJ protein, which functional role in vivo is still unknown. To gain insight into ... Claudin-3 (CLDN3) ist ein ubiquitär exprimiertes TJ-Protein, dessen Rolle in vivo jedoch unbekannt ist. Um Einblicke in dessen ... wurde für diese Arbeit ein Claudin-3-defizientes Mausmodell mittels der konditionalen Gentargeting-Technologie generiert. Zur ... its physiological function a claudin-3 deficient mouse model has been generated using the conditional gene targeting technology ...
3):449-58. doi: 10.1042/BJ20150148. Epub 2015 Apr 7. Comparative Study; Research Support, Non-U.S. Govt ... Human claudin-1 and claudin-3, fused to ECFP or EYFP at the N- or C-terminus, were expressed in the TJ-free cell line HEK ( ... Probing the cis-arrangement of prototype tight junction proteins claudin-1 and claudin-3.. Milatz S1, Piontek J2, Schulzke JD2 ... Using FRET analysis, the proximity of claudin N- and C-termini integrated in homopolymeric strands composed of claudin-3 or of ...
The graph plots aggregation by the particle number at a time point divided by the particle number at time 0 (Nt/No). Claudin-1 ... PKH26-labeled untransfected L-cells (red) were mixed 1:1 with unlabeled untransfected L-cells (top row), GFP-Claudin-1- ... Expression of Claudin-1 or Cacng2 promotes cell aggregation, as shown by microscopy and particle counts. ... Because stargazin is structurally similar to claudins, we hypothesized that it might also have retained claudin-like functions ...
In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant ... Furthermore, decreased expression of claudin-3 was obtained in the colonic epithelium from the c-Kit loss-of-function mutant ... Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression ... We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon ...
Although claudin-3 is present in many epithelia including skin, lung, kidney, and intestine and in endothelia, its function is ... The paracellular barrier of epithelia and endothelia is established by several tight junction proteins including claudin-3. ... In the high-claudin-2 system, claudin-3 reduced claudin-2-induced cation selectivity, while in the low-claudin-2 system no ... Claudin-3 acts as a sealing component of the tight junction for ions of either charge and uncharged solutes Biochim Biophys ...
Claudin 3 human Fusion Protein GST from Proteintech. Produced in E.coli-derived, PET28a, with high quality purity. Cat.No. ... D3-3, No.666 Gaoxin Avenue. Wuhan East Lake Hi-tech Development Zone. Wuhan, Hubei, P.R.C. 027-87531629. 027-87531627 ... Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for ...
... an integral membrane protein which is a component of the claudin family of epithelial tight junction proteins. Claudin-3 is ... It is a low-affinity receptor for Clostridium perfringens enterotoxinin (CPE). Binding of CPE to claudin-3 induces epithelial ... an integral membrane protein which is a component of the claudin family of epithelial tight junction proteins. Claudin-3 is ... an integral membrane protein which is a component of the claudin family of epithelial tight junction proteins. Claudin-3 is ...
Claudin-1 Protein Expression Is a Good Prognositic Factor in Non-Small Cell Lung Cancer, but only in Squamous Cell Carcinoma ... Role of claudin interactions in airway tight junctional permeability. Am J Physiol Lung Cell Mol Physiol. 2003;285:L1166-78 ... Role of claudin interactions in airway tight junctional permeability. Am J Physiol Lung Cell Mol Physiol. 2003;285:L1166-78 ... Decreased expression of claudin-3 is associated with a poor prognosis and EMT in completely resected squamous cell lung ...
Claudin 3 Polyclonal Antibody-NP_001297.1 (MBS241994) product datasheet at MyBioSource, Primary Antibodies. Application: ... Anti-CLDN3 / Claudin 3 Antibody (C-Terminus) IHC-plus; CLDN3; C7orf1; Claudin-3; CPE-R 2; Claudin 3; RVP1; CPE-R2; CPE-receptor ... CLDN3 / Claudin 3 antibody was raised against a synthetic peptide derived from the C-terminus of mouse Claudin 3. Cellular ... 1. Claudin 3 was expressed in all non-goblet columnar lined oesophagus, Barretts oesophagus, high grade dysplasia and ...
show that the epithelial-mesenchymal-transition transcription factor Snail induces claudin-11 expression and suppresses RhoA ... Supplementary Figure 5 Tyrosine-phosphorylated claudin-11 recruits and activates Src.. (a) Amino acid sequence of claudin-11 ( ... Supplementary Figure 7: Expression of Snail or claudin-11 correlates with an advanced HNSCC and a worse prognosis.. ... 7: Expression of Snail or claudin-11 correlates with an advanced HNSCC and a worse prognosis.. ...
... and claudin-low (CL) subtypes. We tested the effects of dietary supplementation with ethyl esters of the marine-derived anti- ... Omega-3-Acid Ethyl Esters Block the Protumorigenic Effects of Obesity in Mouse Models of Postmenopausal Basal-like and Claudin- ... Omega-3-Acid Ethyl Esters Block the Protumorigenic Effects of Obesity in Mouse Models of Postmenopausal Basal-like and Claudin- ... Omega-3-Acid Ethyl Esters Block the Protumorigenic Effects of Obesity in Mouse Models of Postmenopausal Basal-like and Claudin- ...
show that a disease-causing effect of claudin-19 mutations is due to a defective visual cycle from a decreased expression of ... This study explains why claudin-19 mutations alter the expression of RPE signature gene… ... In the kidney, claudin-19 must be complexed with claudin-16 to reach the cell surface27,45. However, human RPE delivers claudin ... ARPE-19 and the kidney cell lines, MDCK and LLC-PK1, do not express claudin-16 or claudin-19. Nonetheless, when claudin-19 is ...
The claudin gene family: expression in normal and neoplastic tissues. BMC Cancer. 2006;6:186.PubMedCentralPubMedCrossRefGoogle ... Claudin-18 gene structure, regulation, and expression is evolutionary conserved in mammals. Gene. 2011;481:83-92.PubMedCrossRef ... Claudin-18 is an early-stage marker of pancreatic carcinogenesis. J Histochem Cytochem. 2011;59:942-952.PubMedCentralPubMed ... UTR and visibly lower the expression of claudin-18. The introduction of claudin-18 without 3′-UTR restored the miR-1303 ...
Claudin-19. A, C. 185. Mus musculus. Mutation(s): 3 Gene Names: Cldn19. ... The claudin-19/C-CPE complex shows no density of a short extracellular helix that is critical for claudins to assemble into TJ ... Here we present the structure of mammalian claudin-19 in complex with C-CPE at 3.7 Å resolution. The structure shows that C-CPE ... CRYSTAL STRUCTURE of MOUSE CLAUDIN-19 IN COMPLEX with C-TERMINAL FRAGMENT OF CLOSTRIDIUM PERFRINGENS ENTEROTOXIN. *DOI: 10.2210 ...
Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis. ... Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis. ... Here, we demonstrated that knockout of Cldn18, a claudin family member highly expressed in lung alveolar epithelium, leads to ... Citation Information: J Clin Invest. 2018;128(3):970-984. https://doi.org/10.1172/JCI90429. View: Text , PDF ...
Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis. ... Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis. ... Here, we demonstrated that knockout of Cldn18, a claudin family member highly expressed in lung alveolar epithelium, leads to ... 3 independent experiments. Input is lung tissue lysate; p-LATS1/2 cannot be detected in input. (. I. ) IP with anti-p-YAP Ab ...
Claudin-20Add BLAST. 219. Proteomic databases. PaxDb, a database of protein abundance averages across all three domains of life ... "Tight junction proteins at the blood-brain barrier: far more than claudin-5.". Berndt P., Winkler L., Cording J., Breitkreuz- ... Belongs to the claudin family.Curated. Keywords - Domaini. Transmembrane, Transmembrane helix. Phylogenomic databases. ... sp,P56880,CLD20_HUMAN Claudin-20 OS=Homo sapiens OX=9606 GN=CLDN20 PE=1 SV=1 ...
J:64941 Morita K, et al., Claudin-11/OSP-based tight junctions of myelin sheaths in brain and Sertoli cells in testis. J Cell ...
"ウサギ・ポリクローナル抗体 ab116165 交差種: Hu 適用: WB,IHC-P…Claudin 3抗体一覧…画像、プロトコール、文献などWeb上の情報 ... All lanes : Anti-Claudin 3 antibody (ab116165) at 1 µg/ml. Lane 1 : Human ovary tissue lysate - total protein (ab30222). Lane 2 ... Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Claudin 3. Immunogen の所有権に関して ... IHC image of Claudin 3 staining in Human normal prostate formalin fixed paraffin
... the presence of claudin 1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to ... the presence of claudin 1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to ... and RAs stained positive with anti-claudin 1 antibodies. Since it has been shown that ameloblasts repeatedly alternate between ... and RAs stained positive with anti-claudin 1 antibodies. Since it has been shown that ameloblasts repeatedly alternate between ...
Claudin 3) ELISA Kit OSCAR DIAGNOSTIC SERVICES PVT. LTD.is an India based Company in Delhi. ... Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit ... Porcine CLDN3 (Claudin 3) ELISA Kit » Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 ( ... Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 (Claudin 3) ELISA Kit Porcine CLDN3 ( ...
Over-expression of claudin-5 was sufficient to prevent replication-deficient virus-induced permeability. The barrier-protective ... Instead, replication-deficient virus induced degradation of the tight junction protein claudin-5; the adherens junction protein ... agent formoterol was able to markedly attenuate flu-induced leak in association with dose-dependent induction of claudin-5. ... Influenza Infects Lung Microvascular Endothelium Leading to Microvascular Leak: Role of Apoptosis and Claudin-5. Figure 10. ...
Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF Resistant Diabetic Macular Edema ... Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF Resistant Diabetic Macular Edema ... Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF Resistant Diabetic Macular Edema ... Claudin-5 Redistribution Induced by Inflammation Leads to Anti-VEGF Resistant Diabetic Macular Edema ...
Claudin-1 Target. Polyclonal Antibody - Claudin-1 Antibody - Immunoprecipitation, Western Blotting, UniProt ID O95832, Entrez ... Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin ... Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin ... Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). The claudin ...
Claudin-1 / CLDN1, 50 µg. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets ... Product Description for Claudin-1 / CLDN1. Rabbit anti Bovine, Canine, Human, Mouse, Porcine, Rat Claudin-1 / CLDN1. ... Claudin-1 / CLDN1. Not available. Human > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column ... Positive controls for Claudin-1 / CLDN1 (3 products). Catalog No.. Species. Pres.. Purity. Source. ...
View mouse Cldn19 Chr4:119255441-119262438 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
Claudin, Fernando, The Communist Movement : From Comintern to Cominform, Monthly Review Press, New York, vol. I, 1975; vol. II ... 1976.. An article from journal Études internationales (Volume 8, Number 3, 1977, pp. 429-534), on Érudit. ... Claudin, Fernando, The Communist Movement : From Comintern to Cominform, Monthly Review Press, New York, vol. I, 1975; vol. II ... "Claudin, Fernando, The Communist Movement : From Comintern to Cominform, Monthly Review Press, New York, vol. I, 1975; vol. II ...
  • Probing the cis-arrangement of prototype tight junction proteins claudin-1 and claudin-3. (nih.gov)
  • The alpha-amino-3-hydroxyl-5-methyl-4-isoxazolepropionate receptor trafficking regulator "stargazin" is related to the claudin family of proteins b. (nih.gov)
  • Stargazin is related to the claudin family of tight junction proteins. (nih.gov)
  • A , the relationship of stargazin (Cacng2) and other Cacng proteins to the claudins is shown in a phylogenetic tree inferred from the amino acid alignment of the 33 indicated human proteins: Clarin-1, Cacng1-8, EMP1-3, PMP-22, LIM2, and 20 claudins (CLDN). (nih.gov)
  • The paracellular barrier of epithelia and endothelia is established by several tight junction proteins including claudin-3. (nih.gov)
  • Clone REA751 recognizes the human claudin-3 antigen, an integral membrane protein which is a component of the claudin family of epithelial tight junction proteins. (miltenyibiotec.com)
  • Besides its role in barrier function, claudin-19 regulates the expression of RPE signature genes 16 , 17 that include visual cycle proteins and retinal neurotrophic factors. (nature.com)
  • Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). (cellsignal.com)
  • The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. (cellsignal.com)
  • Clostridium perfringens enterotoxin (CPE) induces cytolysis very rapidly through binding to its receptors, the tight junction proteins CLDN 3 and 4. (nih.gov)
  • CLDN 3 and 4 proteins were detected in all primary breast carcinomas tested (n = 21) and, compared to normal mammary epithelium, were overexpressed in approximately 62% and 26%, respectively. (nih.gov)
  • The essential membrane proteins discovered to localize to TJs will be the junctional adhesion substances (JAM), occludin as well as the known people from the claudin family members1. (buenavidaestudio.com)
  • At their C-terminus claudins have a PDZ-binding motif, which mediates their conversation with the intracellular scaffolding proteins ZO-1, ZO-2 and ZO-3 linking the claudins to the actin cytoskeleton7. (buenavidaestudio.com)
  • These proteins, such as claudin-5, occludin, and zona occludens 1 (ZO-1) prevent transcellular diffusion of molecules and cells ( 1 ). (frontiersin.org)
  • First, it induces expression of matrix metallopeptidase-9 (MMP-9) and vascular endothelial growth factor (VEGF) in endothelial cells, glial cells and monocytes/macrophages that act to degrade tight junction proteins ( 3 - 5 ). (frontiersin.org)
  • To this end, MDCK C7 cells were stably transfected with either claudin-2 or claudin-10b, two paracellular cation-channel-forming TJ proteins that are not endogenously expressed in this cell line. (biologists.org)
  • Perturbation was overexpression of either claudin-2 or claudin-10b, as both form cation channels within the TJ of MDCK C7 cells, a tight renal epithelial cell line that lacks endogenous expression of these TJ proteins. (biologists.org)
  • Occludin and the sealing tight junction proteins claudin 5 and claudin 8 were downregulated and redistributed off the tight junction, whereas the pore-forming tight junctions protein claudin 2 was strongly upregulated, which constitute the molecular basis of tight junction changes. (bmj.com)
  • Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. (thermofisher.com)
  • TJs are composed of at least three types of transmembrane protein -occludin, claudin and junctional adhesion molecules (JAMs)- and a cytoplasmic 'plaque' consisting of many different proteins that form large complexes. (genome.jp)
  • Claudin 7 belongs to a family of 24 tight junction (TJ) membrane proteins which are central for the regulation of cell permeability and the maintenance of cell polarity. (perkinelmer.com)
  • Cytokine treatment was associated with internalization of AJC transmembrane proteins, junction adhesion molecule 1, occludin, and claudin-1/4 with minimal effects on the cytoplasmic plaque protein zonula occludens 1. (jimmunol.org)
  • Proteins constituting the TJ complex include the transmembrane proteins occludin, claudin (CLD) family members and junctional adhesion molecule (JAM), and linker proteins such as zonula occludens 1 (ZO-1) that affiliate with the underlying actin cytoskeleton ( 8 , 9 , 10 , 11 ). (jimmunol.org)
  • Claudin-low breast cancers are characterized by low expression levels of cell-cell adhesion molecules including E-cadherin and several of the tight junction claudin proteins, claudin 3, 4, and 7. (biomedcentral.com)
  • Recent investigations have focused on tight junction proteins, belonging to the claudin family (claudins-1 to -27 in humans) and the newly defined group of TAMP (three proteins: occludin, Marvel-D2, and tricellulin). (wiley.com)
  • and tight junction proteins, such as Claudin-3, -4 and -7, were selected for further analysis. (hopkinsmedicine.org)
  • CPE binds to the overexpressed tight junction proteins Claudin 3 and 4 on the tumor cells, resulting in tumor cell kill. (hopkinsmedicine.org)
  • 6 , 7 Claudin proteins have four predicted transmembrane helical domains with a short intracellular N-terminus, two extracellular loops, and a long C-terminal tail. (asnjournals.org)
  • His team was the first to propose and demonstrate that the transmembrane claudin proteins create charge-selective pores in the tight junction and that different members of the claudin family confer different ion selectivity. (nih.gov)
  • Proteomic analysis of proteins surrounding occludin and claudin-4 reveals their proximity to signaling and trafficking networks. (nih.gov)
  • Claudin-3 (CLDN3) ist ein ubiquitär exprimiertes TJ-Protein, dessen Rolle in vivo jedoch unbekannt ist. (hu-berlin.de)
  • Claudin-3 (CLDN3) is a ubiquitously expressed TJ protein, which functional role in vivo is still unknown. (hu-berlin.de)
  • Altered expression of claudin-3 (CLDN3), a key cytoskeletal structural protein of the tight junctions in the epithelium, is associated with the development and metastasis of various human cancers. (medsci.org)
  • CLDN3 / Claudin 3 antibody was raised against a synthetic peptide derived from the C-terminus of mouse Claudin 3. (mybiosource.com)
  • Anti-CLDN3 / Claudin 3 antibody IHC of human prostate. (mybiosource.com)
  • The main results are that (i) within homo- and heteropolymers, the average distance between the cytoplasmic ends of the TM1s of cis-interacting claudin molecules is shorter than the average distance between their TM4s, and (ii) TM1 segments of neighbouring claudins are oriented towards each other as the cytoplasmic end of TM1 is in close proximity to more other TM1 segments than TM4 is to other TM4 segments. (nih.gov)
  • Because stargazin is structurally similar to claudins, we hypothesized that it might also have retained claudin-like functions inherited from a common ancestor. (nih.gov)
  • The claudin-19/C-CPE complex shows no density of a short extracellular helix that is critical for claudins to assemble into TJ strands. (rcsb.org)
  • Although some claudins, e.g. claudin-1 and claudin-3 type paracellular barriers, various other claudins, e.g. claudin-2 or claudin-16, form paracellular pores allowing for controlled diffusion of ions and water via the TJs5. (buenavidaestudio.com)
  • Additional claudins reported to be present in BBB TJs are claudin-3 and claudin-12 with their precise functions in BBB TJs to be decided12C15. (buenavidaestudio.com)
  • Upregulation of pore-forming claudin 2 and downregulation and redistribution of sealing claudins 5 and 8 lead to altered tight junction structure and pronounced barrier dysfunction already in mild to moderately active Crohn's disease. (bmj.com)
  • Targeting genes that were found by our previous SAGE analysis searches to be overexpressed in breast cancer (the Claudins 3 and 4), we are testing therapies using Clostridium perfringens enterotoxin (CPE). (hopkinsmedicine.org)
  • Unexpectedly, the anti-claudin-1 antibody localized claudin to the Golgi apparatus of a sub-population of SA and RA in addition to the distal junctions of RA. (frontiersin.org)
  • The following product was used in this experiment: Claudin 4 Monoclonal Antibody (3E2C1) from Thermo Fisher Scientific, catalog # 32-9400, RRID AB_2533096. (thermofisher.com)
  • This antibody reacts specifically with the ~ 22 kDa Claudin-4 protein and does not cross-react with the protein at 55 kDa. (thermofisher.com)
  • Recent antibody-based studies indicated that Claudin 7 is not only localized at the apical TJs but also have a strong basolateral membrane distribution in the epithelia of various tissues. (perkinelmer.com)
  • Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in human cell lines SK-MEL-30 and HEK293 using Anti-ARPC1B antibody. (novusbio.com)
  • Immunohistochemistry-Paraffin: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Staining of human lymph node shows strong cytoplasmic positivity in lymphoid cells outside reaction centra. (novusbio.com)
  • Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II. (novusbio.com)
  • Western Blot: actin-related protein 2/3 complex subunit 1B Antibody [NBP1-90114] - Analysis in human cell line RT-4. (novusbio.com)
  • Immunohistochemistry (IHC) analyzes of Claudin-1 antibody (Cat. (acris-antibodies.com)
  • Western blot (WB) analysis of Claudin-1 antibody (Cat. (acris-antibodies.com)
  • The modulating agents comprise at least one claudin cell adhesion recognition sequence or an antibody or fragment thereof that specifically binds the claudin cell adhesion recognition sequence. (justia.com)
  • The present invention relates generally to methods for regulating claudin-mediated processes, and more particularly to the use of modulating agents comprising a claudin cell adhesion recognition sequence and/or an antibody that specifically recognizes such a sequence for inhibiting functions such as cell adhesion and the formation of tissue permeability barriers. (justia.com)
  • Effects of claudin-2, occludin or ZO-1 siRNA transfection on transepithelial electrical resistance (TER) in HK-2 cell monolayers. (figshare.com)
  • TER was significantly decreased by claudin-2 siRNA transfection, but significantly increased by siRNA transfection against occludin or ZO-1. (figshare.com)
  • To gain insight into its physiological function a claudin-3 deficient mouse model has been generated using the conditional gene targeting technology. (hu-berlin.de)
  • The protein encoded by this intronless gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. (mybiosource.com)
  • However, the effects of microRNA-1303 (miR-1303) on gastric cancer (GC) cells and the upstream regulation of GC-associated claudin-18 gene (CLDN18) remain unclear. (springer.com)
  • The claudin gene family: expression in normal and neoplastic tissues. (springer.com)
  • The following are some of the gene count estimates of human chromosome 3. (wikipedia.org)
  • One of these, claudin-4, is a member of the claudin gene family that has been identified in several independent gene expression profiles to be up-regulated in a variety of tumors ( 8 - 13 ) and, importantly, in premalignant lesions of the pancreas ( 14 ) and esophagus ( 15 ). (aacrjournals.org)
  • The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems. (thermofisher.com)
  • This gene encodes a member of the claudin family. (genecards.org)
  • CLDN18 (Claudin 18) is a Protein Coding gene. (genecards.org)
  • CLDN15 (Claudin 15) is a Protein Coding gene. (genecards.org)
  • In addition, Snail and Slug are able to effectively repress human Claudin - 1 -driven reporter gene constructs containing the wild-type promoter sequence, but not those with mutations in two proximal E-box elements. (biochemj.org)
  • Taken together, these results support the hypothesis that Claudin - 1 is a direct downstream target gene of Snail family factors in epithelial cells. (biochemj.org)
  • The nucleotide sequence data reported for Canis familiaris partial mRNA for putative claudin-1 protein ( cldn1 gene) has been submitted to DDBJ, EMBL, GenBank® and GSDB Nucleotide Sequence Databases under the accession number AJ628857 . (biochemj.org)
  • Although many aspects of drug MoA can be uncovered through analysis of drug-perturbed gene expression in CCLs [ 3 , 5 , 17 - 19 ], analysis of basal gene expression is valuable because it sheds light on the relationship between the cell's resting physiological state and its drug sensitivity. (biomedcentral.com)
  • found that an activating mutation in the catalytic α subunit of PI3K enhanced the expression of a gene encoding pentraxin-3, a protein that functions in the innate immune response. (sciencemag.org)
  • Using transcriptomic analysis of activated PIK3CA-expressing BLBC cells, we identified the gene encoding the humoral pattern recognition molecule pentraxin-3 (PTX3) as a critical target of oncogenic PI3K signaling. (sciencemag.org)
  • We conclude that 2,3- pentanedione is a respiratory hazard that can also alter gene expression in the brain. (cdc.gov)
  • miR-1303 could bind to the putative binding sites in CLDN18 mRNA 3′-UTR and visibly lower the expression of claudin-18. (springer.com)
  • Here, we demonstrated that knockout of Cldn18, a claudin family member highly expressed in lung alveolar epithelium, leads to lung enlargement, parenchymal expansion, increased abundance and proliferation of known distal lung progenitors, the alveolar epithelial type II (AT2) cells, activation of Yes-associated protein (YAP), increased organ size, and tumorigenesis in mice. (jci.org)
  • CLDN18 overexpression prevents increases in colony number ( A ) and size ( B ). n = 3 independent experiments. (jci.org)
  • H ) IP with anti-p-LATS1/2 Ab shows decreased p-LATS1/2 and decreased association of p-LATS1/2 with p-YAP in Cldn18 -/- (lane 4) compared with WT (lane 3) AT2 cell membranes. (jci.org)
  • Auf www.antikoerper-online.de finden Sie aktuell 60 Claudin 18 (CLDN18) Antikörper von 14 unterschiedlichen Herstellern. (antikoerper-online.de)
  • Under all conditions, water flux in claudin-2-transfected cells was elevated compared with vector controls, indicating claudin-2-mediated paracellular water permeability. (biologists.org)
  • The overexpression of Claudin-4 has been shown to decrease paracellular electrical conductance due to a selective decrease in Na+ permeability, with no significant decrease in Cl - permeability. (thermofisher.com)
  • While some claudin family members function as impermeable barriers, others mediate the permeability to ions and small molecules. (genecards.org)
  • Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. (genecards.org)
  • Abnormal mucosal permeability has been observed in patients with inflammatory bowel disease (IBD), 3 where patients present with relapsing diarrhea attributed to inflammation and altered epithelial paracellular permeability ( 1 , 2 , 3 , 4 , 5 ). (jimmunol.org)
  • Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. (mdpi.com)
  • We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. (mdpi.com)
  • In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. (mdpi.com)
  • Furthermore, decreased expression of claudin-3 was obtained in the colonic epithelium from the c-Kit loss-of-function mutant mice. (mdpi.com)
  • In conclusion, SCF/c-kit-JNK/AP-1 signaling pathway significantly promoted claudin-3 expression in colonic epithelium and CRC, which could contribute to epithelial barrier function maintenance and to CRC development. (mdpi.com)
  • Two clone systems were analyzed, exhibiting high or low claudin-2 expression, respectively. (nih.gov)
  • We show that the EMT transcription factor Snail elicits collective migration in squamous cell carcinoma by inducing the expression of a tight junctional protein, claudin-11. (nature.com)
  • Exogenous expression of wild type, but not mutant claudin-19, increases the expression of RPE signature genes. (nature.com)
  • The expression of miR-1303 and claudin-18 in GC tissues and gastric cancer cell lines were detected by qRT-PCR and western blotting, respectively. (springer.com)
  • Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). (cellsignal.com)
  • We hypothesize that 3-chloropropanediol-induced modulation of tight junction protein expression is mediated through the phosphoinositide-3-kinase (PI3K)/AKT pathway. (aspetjournals.org)
  • Results showed early reversible loss of sharp paracellular claudin-5 expression 90, 105, and 120 min following 3-chloropropanediol (500 μM) treatment. (aspetjournals.org)
  • Western blot and immunofluorescence studies showed increased p85-PI3K expression and transitory increased AKT (Thr308) phosphorylation at 15 and 30 min after 3-chloropropanediol administration. (aspetjournals.org)
  • However, AKT inhibitors only prevented the early changes in claudin-5 expression. (aspetjournals.org)
  • This mechanistic study provides a greater understanding of the intracellular signaling pathways mediating tight junction protein expression and supports a hypothesis that two independent pathways triggered by PI3K mediate early and late loss of paracellular claudin-5 expression. (aspetjournals.org)
  • In this study, we investigated CLDN 3 and 4 expression in breast cancer and tested the potential of CPE-mediated therapy. (nih.gov)
  • Thus, expression of CLDN 3 and 4 sensitizes primary breast carcinomas to CPE-mediated cytolysis and emphasizes the potential of CPE in breast cancer therapy. (nih.gov)
  • In mammals, the claudin family members comprises 27 known people that display tissues particular expression patterns and various functions. (buenavidaestudio.com)
  • Jun, Kim, Jung, Choi, Chin: Expression of claudin-7 and loss of claudin-18 correlate with poor prognosis in gastric cancer. (antikoerper-online.de)
  • Significantly, in invasive human breast tumours, high levels of Snail and Slug correlated with low levels of Claudin - 1 expression. (biochemj.org)
  • Abnormal expression and mislocalization of Claudin 7 are frequently observed in epithelial-derived lung, breast, head, and neck cancers. (perkinelmer.com)
  • High GLI1 expression is a property of claudin-low cells and tumors and correlates with markers of EMT and breast cancer stem cells. (biomedcentral.com)
  • Knockdown of GLI1 expression in claudin-low cell lines resulted in reduced cell viability, motility, clonogenicity, self-renewal, and reduced tumor growth of orthotopic xenografts. (biomedcentral.com)
  • Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. (acris-antibodies.com)
  • 2019. ADAM15 mediates upregulation of Claudin-1 expression in breast cancer cells . (cardiff.ac.uk)
  • Olfactory neuroepithelial injury included loss of olfactory neurons that showed reduced expression of the 2,3- pentanedione -metabolizing enzyme, dicarbonyl/L-xylulose reductase, relative to sustentacular cells. (cdc.gov)
  • An additional group of rats inhaling 270 ppm 2,3- pentanedione for 6 hours 41 minutes showed increased expression of IL-6 and nitric oxide synthase-2 and decreased expression of vascular endothelial growth factor A in the OB, striatum, hippocampus, and cerebellum using real-time PCR. (cdc.gov)
  • Claudin-1 expression increased in the OB and striatum. (cdc.gov)
  • The homology of claudin-4 to Clostridium perfringens enterotoxin (CPE) receptor suggests that this protein may be a potential target for therapeutic intervention and, indeed, preliminary studies in cell lines and xenografts have shown some promise in this regard ( 16 , 17 ). (aacrjournals.org)
  • Manche β-PFT wie das Clostridium ε-Toxin und das Clostridium perfringens Enterotoxin (CPE) binden an Rezeptoren , vermutlich Claudin bei CPE, [14] sowie vermutlich GPI-Anker oder andere Glykosylierungen beim ε-Toxin. (wikipedia.org)
  • Overexpression of snail correlates with increased claudin-11, and both are associated with a worse outcome. (nature.com)
  • Overexpression of Slug and Snail in MDCK (Madin-Darby canine kidney) cells down-regulated Claudin-1 at protein and mRNA levels. (biochemj.org)
  • Although claudin-3 is present in many epithelia including skin, lung, kidney, and intestine and in endothelia, its function is unresolved as yet. (nih.gov)
  • Such changes of the airway epithelia eventually progress to low- or high-grade dysplastic lesions and malignant transformation ( in situ carcinoma and invasive lung cancer) [ 3 - 5 ] . (medsci.org)
  • Claudin-2 is typical of leaky, water-transporting epithelia, such as the kidney proximal tubule, whereas claudin-10b is present in numerous epithelia, including water-impermeable segments of the loop of Henle. (biologists.org)
  • We conclude that claudin-2, but not claudin-10b, forms a paracellular water channel and thus mediates paracellular water transport in leaky epithelia. (biologists.org)
  • Our results uncover crosstalk between NFκB and GLI1 signals and suggest that targeting these pathways may be effective against the claudin-low breast cancer subtype. (biomedcentral.com)
  • miR-1303 was significantly overexpressed whereas claudin-18 was downregulated in GC tissues and cell lines, which was significantly associated with tumor size, location invasion, histologic type and tumor-node-metastasis stage. (springer.com)
  • 51.5% of gastric cancer tissues had reduced claudin-18 . (antikoerper-online.de)
  • Claudin-4 is also overexpressed in pancreatic cancer tissues and cell lines, and in other epithelial cancers. (thermofisher.com)
  • Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. (mdpi.com)
  • We demonstrate that claudin-3 alters the tight junction meshwork and seals the paracellular pathway against the passage of small ions of either charge and uncharged solutes. (nih.gov)
  • Claudin-3 is involved in tight junction-specific obliteration of the intercellular space. (miltenyibiotec.com)
  • 1999) Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. (miltenyibiotec.com)
  • Claudin-3: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. (mybiosource.com)
  • Claudin is the major component of tight junction and polymerizes to form tight junction strands with various morphologies that may correlate with their functions. (elsevier.com)
  • The presence or absence of the helix bending changes the positions of residues related to claudin-claudin interactions and affects the morphology and adhesiveness of the tight junction strands. (elsevier.com)
  • Claudin-11/OSP-based tight junctions of myelin sheaths in brain and Sertoli cells in testis. (jax.org)
  • Claudin-1 is a tight junction transmembrane protein. (frontiersin.org)
  • Since it has been shown that ameloblasts repeatedly alternate between an SA and an RA morphology during enamel maturation, the presence of claudin-1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to the junctional area. (frontiersin.org)
  • Localization of claudin-1 in the Golgi apparatus may reflect rapid tight junction turnover during enamel maturation. (frontiersin.org)
  • We have previously shown that systemic administration of 3-chloropropanediol induces a transitory glial fibrillary acidic protein (GFAP)-astrocyte loss, reversible loss of tight junction complexes, and BBB integrity disruption. (aspetjournals.org)
  • The presented data substantiate the hypothesis that claudin-18 is a central barrier-forming component of tight junctions and show that IL-13 (zeige IL13 Antikörper ) downregulates claudin-18 . (antikoerper-online.de)
  • Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. (thermofisher.com)
  • Claudin-1 is an integral membrane protein component of tight junctions. (biochemj.org)
  • Mechanistically, tyrosine-phosphorylated claudin-11 activates Src, which suppresses RhoA activity at intercellular junctions through p190RhoGAP, maintaining stable cell-cell contacts. (nature.com)
  • Claudin-5 forms a paracellular barrier as its constitutive lack prospects to perinatal death in mice due to the uncontrolled diffusion of small molecules across BBB TJs8 and induced suppression of claudin-5 in adult mice prospects to seizures and death11. (buenavidaestudio.com)
  • Here we present the crystal structure of mammalian claudin-3 at 3.6 Å resolution. (elsevier.com)
  • There are 27 mammalian claudin genes, although the homology and nomenclature of the more distantly related genes are not completely settled. (asnjournals.org)
  • In ovarian carcinoma cells, Claudin-3 and Claudin-4 exhibit upregulation at both mRNA and protein levels. (thermofisher.com)
  • The findings link innate immune signaling with breast cancer development and suggest that targeting pentraxin-3 may suppress tumor growth in a subset of patients. (sciencemag.org)
  • Distal junction area of ruffle-ended ameloblasts (RA) and the Golgi apparatus of a sub-population of smooth-ended ameloblasts (SA) and RAs stained positive with anti-claudin-1 antibodies. (frontiersin.org)
  • Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). (cellsignal.com)
  • Treatment of breast cancer cell lines in culture with CPE resulted in rapid and dose-dependent cytolysis exclusively in cells that expressed CLDN 3 and 4. (nih.gov)
  • Moreover, an inverse correlation in the levels of Claudin - 1 and Slug transcripts were observed in breast cancer cell lines. (biochemj.org)
  • E-box elements in the Claudin - 1 promoter were found to play a critical negative regulatory role in breast cancer cell lines that expressed low levels of Claudin - 1 transcript. (biochemj.org)
  • 2016. The clinical implications of RSK1-3 in human breast cancer . (cardiff.ac.uk)
  • The recently identified claudin-low subtype of breast cancer is enriched for cells with stem-like and mesenchymal-like characteristics. (biomedcentral.com)
  • Furthermore, an increase in the abundance of pentraxin-3 promoted stem cell-like traits in mammary epithelial and breast cancer cells. (sciencemag.org)
  • Binding of CPE to claudin-3 induces epithelial cell lysis. (miltenyibiotec.com)
  • Obesity induces chronic inflammation and is an established risk and progression factor for triple-negative breast cancers, including basal-like (BL) and claudin-low (CL) subtypes. (aacrjournals.org)
  • On 3 - 5 October, 2018, we organized the XXV. (guarant.cz)
  • Claudin 2 upregulation was less pronounced in active Crohn's disease compared with active ulcerative colitis and was inducible by tumour necrosis factor α. (bmj.com)
  • Human claudin-1 and claudin-3, fused to ECFP or EYFP at the N- or C-terminus, were expressed in the TJ-free cell line HEK (human embryonic kidney)-293. (nih.gov)
  • We therefore characterized claudin-3 by stable transfection of MDCK II kidney tubule cells with human claudin-3 cDNA. (nih.gov)
  • Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Claudin 3. (abcam.co.jp)
  • Human chromosome 3 pair after G-banding . (wikipedia.org)
  • Chromosome 3 pair in human male karyogram . (wikipedia.org)
  • The following is a partial list of genes on human chromosome 3. (wikipedia.org)
  • the BBB and demonstrates lack of claudin-3 will not impair human brain hurdle function during health insurance and PLX4032 inhibitor neuroinflammation in C57BL/6J mice. (buenavidaestudio.com)
  • Additional proof for a job of claudin-3 in human brain hurdle integrity is derived from its recognition like a downstream effector. (buenavidaestudio.com)
  • Synthetic peptide corresponding to a 22 amino acid sequence derived from the C-terminal region of human Claudin-4. (thermofisher.com)
  • We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin - 1 promoter. (biochemj.org)
  • The AlphaLISA ® immunoassay kit for human claudin 7 enables the detection and quantitation of claudin 7 in cell culture media and human cell lysates using a homogeneous AlphaLISA assay (no wash steps). (perkinelmer.com)
  • There are over 3 million human Expressed Sequence Tag (EST) records in GenBank (Table 1 ), which is still growing rapidly. (biomedcentral.com)
  • Together, Dr. Anderson's claudin research has provided significant insight toward our current knowledge regarding the molecular basis of selective paracellular transport and inherited human defects of the junction barrier. (nih.gov)
  • The data provide novel insight into the molecular TJ architecture consistent with a model with an antiparallel double-row cis-arrangement of classic claudin protomers within strands. (nih.gov)
  • 3 Cologne Excellence Cluster on Cellular Stress Responses in Aging-Associated Diseases (CECAD) and Center for Molecular Medicine Cologne (CMMC), University of Cologne, Cologne, Germany. (jci.org)
  • In primary cancers, 123 of 125 (98.4%) were positive for claudin-4, 116 of 126 (94%) for MKK4, and 111 of 120 (92%) for stratifin. (aacrjournals.org)
  • Measuring the Claudin 7 levels is important for the detection of different types of cancers and is also an important target for therapeutic intervention. (perkinelmer.com)
  • The phosphoinositide 3-kinase (PI3K) pathway is activated in various cancers. (sciencemag.org)
  • Basal-like breast cancers (BLBCs) exhibit hyperactivation of the phosphoinositide 3-kinase (PI3K) signaling pathway because of the frequent mutational activation of the PIK3CA catalytic subunit and the genetic loss of its negative regulators PTEN (phosphatase and tensin homolog) and INPP4B (inositol polyphosphate-4-phosphatase type II). (sciencemag.org)
  • To test this hypothesis we have used a mouse brain endothelial cell line (bEnd.3) exposed to 3-chloropropanediol for up to 3 days. (aspetjournals.org)
  • In mice, claudin-19 was found in the kidney and Schwan cells of the peripheral nervous system, but was absent from the oligodendrocytes of the central nervous system 4 . (nature.com)
  • In mammals, humans have 23 claudin genes, whereas rats and mice have 24 genes ( Lal-Nag and Morin, 2009 ). (frontiersin.org)
  • Some mice infected with live influenza were given formoterol (0.2 mg/kg) or vehicle by intraperitoneal injection immediately afterwards and daily for 3 days. (plos.org)
  • Because TJs of basal cells are primarily composed of claudin-11, claudin-11-deficient (Cld11 -/- ) mice were generated with an expectation that the compartmentalization in stria vascularis in these mice would be affected. (biologists.org)
  • 2013. Tissue inhibitor of metalloproteinases-3 peptides inhibit angiogenesis and choroidal neovascularization in mice . (cardiff.ac.uk)
  • By contrast, claudin-10b transfection did not alter water flux. (biologists.org)
  • In the high-claudin-2 system, claudin-3 reduced claudin-2-induced cation selectivity, while in the low-claudin-2 system no charge preference was observed, the latter thus reflecting the "intrinsic" action of claudin-3. (nih.gov)
  • Thus, in a kidney model epithelium, claudin-3 acts as a general barrier-forming protein. (nih.gov)
  • Chromatin immunoprecipitation (ChIP) and luciferase reporter assay showed that SCF/c-kit signaling significantly promoted activator protein-1 (AP-1) binding with CLDN-3 promoter and enhanced its transcription activity. (mdpi.com)
  • We further provide direct evidence for the binding of the NFκB subunit p65 to the GLI1 promoter in both EMT and claudin-low cell lines. (biomedcentral.com)
  • Using FRET analysis, the proximity of claudin N- and C-termini integrated in homopolymeric strands composed of claudin-3 or of heteropolymeric strands composed of claudin-1 and claudin-3 were determined. (nih.gov)
  • Claudin-5 is an endothelial cell-specific component of TJ strands and it is highly expressed in BBB TJs of rodents, zebrafish, nonhuman primates and humans8C10. (buenavidaestudio.com)
  • Finally, claudin-11 is responsible for the induction of the unique parallel TJ strands observed in choroid plexus epithelial cells16. (buenavidaestudio.com)
  • Most cell types express more than two claudin species in various combinations to constitute TJ strands. (biologists.org)
  • Chromosome 3 spans almost 200 million base pairs (the building material of DNA ) and represents about 6.5 percent of the total DNA in cells . (wikipedia.org)
  • Bile duct adenocarcinoma cells overexpress claudin-18 via the EGFR (zeige EGFR Antikörper )/ RAS / ERK (zeige EPHB2 Antikörper ) pathway, contributing to cell proliferation and invasion. (antikoerper-online.de)
  • ARPE-19 cells at passages p22 to p28 were cultured on filters for up to 3 months in DMEM/F12 or DMEM media with or without pyruvate and 1% fetal calf serum. (arvojournals.org)
  • Claudin-low tumors are mainly triple-negative invasive ductal carcinomas with a high frequency of metaplastic and medullary differentiation. (biomedcentral.com)