A large family of transmembrane proteins found in TIGHT JUNCTIONS. They take part in the formation of paracellular barriers and pores that regulate paracellular permeability.
A ubiquitously-expressed claudin subtype that acts as a general barrier-forming protein in TIGHT JUNCTIONS. Elevated expression of claudin-3 is found in a variety of tumor cell types, suggesting its role as a therapeutic target for specific ANTINEOPLASTIC AGENTS.
An integral membrane protein that is localized to TIGHT JUNCTIONS, where it plays a role in controlling the paracellular permeability of polarized cells. Mutations in the gene for claudin-1 are associated with Neonatal Ichthyosis-Sclerosing Cholangitis (NISCH) Syndrome.
A claudin subtype that takes part in maintaining the barrier-forming property of TIGHT JUNCTIONS. Claudin-4 is found associated with CLAUDIN-8 in the KIDNEY COLLECTING DUCT where it may play a role in paracellular chloride ion reabsorption.
Cell-cell junctions that seal adjacent epithelial cells together, preventing the passage of most dissolved molecules from one side of the epithelial sheet to the other. (Alberts et al., Molecular Biology of the Cell, 2nd ed, p22)
A claudin subtype that is found localized to TIGHT JUNCTIONS in VASCULAR ENDOTHELIAL CELLS. The protein was initially identified as one of several proteins which are deleted in VELOCARDIOFACIAL SYNDROME and may play an important role in maintaining the integrity of the BLOOD-BRAIN BARRIER.
Proteins which are found in membranes including cellular and intracellular membranes. They consist of two types, peripheral and integral proteins. They include most membrane-associated enzymes, antigenic proteins, transport proteins, and drug, hormone, and lectin receptors.
A MARVEL domain protein that plays an important role in the formation and regulation of the TIGHT JUNCTION paracellular permeability barrier.
A 195-kDa zonula occludens protein that is distinguished by the presence of a ZU5 domain at the C-terminal of the molecule.
A zonula occludens protein subtype found in epithelial cell junctions. Several isoforms of zonula occludens-2 protein exist due to use of alternative promoter regions and alternative mRNA splicings.
A claudin subtype that is associated with the formation of cation-selective channels and increased epithelial permeability. It is localized to the TIGHT JUNCTIONS of the PROXIMAL KIDNEY TUBULE and INTESTINAL EPITHELIUM.
A specialized barrier, in the TESTIS, between the interstitial BLOOD compartment and the adluminal compartment of the SEMINIFEROUS TUBULES. The barrier is formed by layers of cells from the VASCULAR ENDOTHELIUM of the capillary BLOOD VESSELS, to the SEMINIFEROUS EPITHELIUM of the seminiferous tubules. TIGHT JUNCTIONS form between adjacent SERTOLI CELLS, as well as between the ENDOTHELIAL CELLS.
Property of membranes and other structures to permit passage of light, heat, gases, liquids, metabolites, and mineral ions.
The resistance to the flow of either alternating or direct electrical current.
The technique of placing cells or tissue in a supporting medium so that thin sections can be cut using a microtome. The medium can be paraffin wax (PARAFFIN EMBEDDING) or plastics (PLASTIC EMBEDDING) such as epoxy resins.
A genus of pufferfish commonly used for research.
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
Excretion of abnormally high level of CALCIUM in the URINE, greater than 4 mg/kg/day.
An inherited renal disorder characterized by defective NaCl reabsorption in the convoluted DISTAL KIDNEY TUBULE leading to HYPOKALEMIA. In contrast with BARTTER SYNDROME, Gitelman syndrome includes hypomagnesemia and normocalcemic hypocalciuria, and is caused by mutations in the thiazide-sensitive SODIUM-POTASSIUM-CHLORIDE SYMPORTERS.
A condition characterized by calcification of the renal tissue itself. It is usually seen in distal RENAL TUBULAR ACIDOSIS with calcium deposition in the DISTAL KIDNEY TUBULES and the surrounding interstitium. Nephrocalcinosis causes RENAL INSUFFICIENCY.
A quality of cell membranes which permits the passage of solvents and solutes into and out of cells.
Human colonic ADENOCARCINOMA cells that are able to express differentiation features characteristic of mature intestinal cells, such as ENTEROCYTES. These cells are valuable in vitro tools for studies related to intestinal cell function and differentiation.
The U-shaped portion of the renal tubule in the KIDNEY MEDULLA, consisting of a descending limb and an ascending limb. It is situated between the PROXIMAL KIDNEY TUBULE and the DISTAL KIDNEY TUBULE.
Preparation for electron microscopy of minute replicas of exposed surfaces of the cell which have been ruptured in the frozen state. The specimen is frozen, then cleaved under high vacuum at the same temperature. The exposed surface is shadowed with carbon and platinum and coated with carbon to obtain a carbon replica.
Cells of epithelial origin possessing specialized sensory functions. They include cells that are found in the TASTE BUDS; OLFACTORY MUCOSA; COCHLEA; and NEUROEPITHELIAL BODIES.
One or more layers of EPITHELIAL CELLS, supported by the basal lamina, which covers the inner or outer surfaces of the body.
Paired respiratory organs of fishes and some amphibians that are analogous to lungs. They are richly supplied with blood vessels by which oxygen and carbon dioxide are exchanged directly with the environment.
The domestic dog, Canis familiaris, comprising about 400 breeds, of the carnivore family CANIDAE. They are worldwide in distribution and live in association with people. (Walker's Mammals of the World, 5th ed, p1065)
Anchoring points where the CYTOSKELETON of neighboring cells are connected to each other. They are composed of specialized areas of the plasma membrane where bundles of the ACTIN CYTOSKELETON attach to the membrane through the transmembrane linkers, CADHERINS, which in turn attach through their extracellular domains to cadherins in the neighboring cell membranes. In sheets of cells, they form into adhesion belts (zonula adherens) that go all the way around a cell.
Supporting cells projecting inward from the basement membrane of SEMINIFEROUS TUBULES. They surround and nourish the developing male germ cells and secrete ANDROGEN-BINDING PROTEIN and hormones such as ANTI-MULLERIAN HORMONE. The tight junctions of Sertoli cells with the SPERMATOGONIA and SPERMATOCYTES provide a BLOOD-TESTIS BARRIER.
A usually benign glandular tumor composed of oxyphil cells, large cells with small irregular nuclei and dense acidophilic granules due to the presence of abundant MITOCHONDRIA. Oxyphil cells, also known as oncocytes, are found in oncocytomas of the kidney, salivary glands, and endocrine glands. In the thyroid gland, oxyphil cells are known as Hurthle cells and Askanazy cells.
Histochemical localization of immunoreactive substances using labeled antibodies as reagents.
Direct contact of a cell with a neighboring cell. Most such junctions are too small to be resolved by light microscopy, but they can be visualized by conventional or freeze-fracture electron microscopy, both of which show that the interacting CELL MEMBRANE and often the underlying CYTOPLASM and the intervening EXTRACELLULAR SPACE are highly specialized in these regions. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p792)
Established cell cultures that have the potential to propagate indefinitely.
Different forms of a protein that may be produced from different GENES, or from the same gene by ALTERNATIVE SPLICING.
The artificial induction of GENE SILENCING by the use of RNA INTERFERENCE to reduce the expression of a specific gene. It includes the use of DOUBLE-STRANDED RNA, such as SMALL INTERFERING RNA and RNA containing HAIRPIN LOOP SEQUENCE, and ANTI-SENSE OLIGONUCLEOTIDES.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action during the developmental stages of an organism.
Body organ that filters blood for the secretion of URINE and that regulates ion concentrations.
A form of fluorescent antibody technique commonly used to detect serum antibodies and immune complexes in tissues and microorganisms in specimens from patients with infectious diseases. The technique involves formation of an antigen-antibody complex which is labeled with fluorescein-conjugated anti-immunoglobulin antibody. (From Bennington, Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)
Degree of saltiness, which is largely the OSMOLAR CONCENTRATION of SODIUM CHLORIDE plus any other SALTS present. It is an ecological factor of considerable importance, influencing the types of organisms that live in an ENVIRONMENT.
The simultaneous analysis of multiple samples of TISSUES or CELLS from BIOPSY or in vitro culture that have been arranged in an array format on slides or microchips.
The most common etiologic agent of GAS GANGRENE. It is differentiable into several distinct types based on the distribution of twelve different toxins.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A general term for the complete or partial loss of the ability to hear from one or both ears.
RNA sequences that serve as templates for protein synthesis. Bacterial mRNAs are generally primary transcripts in that they do not require post-transcriptional processing. Eukaryotic mRNA is synthesized in the nucleus and must be exported to the cytoplasm for translation. Most eukaryotic mRNAs have a sequence of polyadenylic acid at the 3' end, referred to as the poly(A) tail. The function of this tail is not known for certain, but it may play a role in the export of mature mRNA from the nucleus as well as in helping stabilize some mRNA molecules by retarding their degradation in the cytoplasm.
Lining of the INTESTINES, consisting of an inner EPITHELIUM, a middle LAMINA PROPRIA, and an outer MUSCULARIS MUCOSAE. In the SMALL INTESTINE, the mucosa is characterized by a series of folds and abundance of absorptive cells (ENTEROCYTES) with MICROVILLI.
The part of the inner ear (LABYRINTH) that is concerned with hearing. It forms the anterior part of the labyrinth, as a snail-like structure that is situated almost horizontally anterior to the VESTIBULAR LABYRINTH.
Phenotypic changes of EPITHELIAL CELLS to MESENCHYME type, which increase cell mobility critical in many developmental processes such as NEURAL TUBE development. NEOPLASM METASTASIS and DISEASE PROGRESSION may also induce this transition.
The portion of renal tubule that begins from the enlarged segment of the ascending limb of the LOOP OF HENLE. It reenters the KIDNEY CORTEX and forms the convoluted segments of the distal tubule.
Substances that are toxic to the intestinal tract causing vomiting, diarrhea, etc.; most common enterotoxins are produced by bacteria.
Male germ cells derived from SPERMATOGONIA. The euploid primary spermatocytes undergo MEIOSIS and give rise to the haploid secondary spermatocytes which in turn give rise to SPERMATIDS.
Membrane-limited structures derived from the plasma membrane or various intracellular membranes which function in storage, transport or metabolism.
A malignant neoplasm arising from tenosynovial tissue of the joints and in synovial cells of tendons and bursae. The legs are the most common site, but the tumor can occur in the abdominal wall and other trunk muscles. There are two recognized types: the monophasic (characterized by sheaths of monotonous spindle cells) and the biphasic (characterized by slit-like spaces or clefts within the tumor, lined by cuboidal or tall columnar epithelial cells). These sarcomas occur most commonly in the second and fourth decades of life. (From Dorland, 27th ed; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, p1363)
A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control (induction or repression) of gene action at the level of transcription or translation.
Cells propagated in vitro in special media conducive to their growth. Cultured cells are used to study developmental, morphologic, metabolic, physiologic, and genetic processes, among others.
The mucous membrane lining the RESPIRATORY TRACT, including the NASAL CAVITY; the LARYNX; the TRACHEA; and the BRONCHI tree. The respiratory mucosa consists of various types of epithelial cells ranging from ciliated columnar to simple squamous, mucous GOBLET CELLS, and glands containing both mucous and serous cells.
Carcinoma that arises from the PANCREATIC DUCTS. It accounts for the majority of cancers derived from the PANCREAS.
Molecular products metabolized and secreted by neoplastic tissue and characterized biochemically in cells or body fluids. They are indicators of tumor stage and grade as well as useful for monitoring responses to treatment and predicting recurrence. Many chemical groups are represented including hormones, antigens, amino and nucleic acids, enzymes, polyamines, and specific cell membrane proteins and lipids.
The determination of the pattern of genes expressed at the level of GENETIC TRANSCRIPTION, under specific circumstances or in a specific cell.
An EPITHELIUM with MUCUS-secreting cells, such as GOBLET CELLS. It forms the lining of many body cavities, such as the DIGESTIVE TRACT, the RESPIRATORY TRACT, and the reproductive tract. Mucosa, rich in blood and lymph vessels, comprises an inner epithelium, a middle layer (lamina propria) of loose CONNECTIVE TISSUE, and an outer layer (muscularis mucosae) of SMOOTH MUSCLE CELLS that separates the mucosa from submucosa.
Stratified squamous epithelium that covers the outer surface of the CORNEA. It is smooth and contains many free nerve endings.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The physical or physiological processes by which substances, tissue, cells, etc. take up or take in other substances or energy.
Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.
The sequential location of genes on a chromosome.
Specialized non-fenestrated tightly-joined ENDOTHELIAL CELLS with TIGHT JUNCTIONS that form a transport barrier for certain substances between the cerebral capillaries and the BRAIN tissue.
The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.
Small polyhedral outpouchings along the walls of the alveolar sacs, alveolar ducts and terminal bronchioles through the walls of which gas exchange between alveolar air and pulmonary capillary blood takes place.
Proteins obtained from the ZEBRAFISH. Many of the proteins in this species have been the subject of studies involving basic embryological development (EMBRYOLOGY).
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
Calcium-dependent cell adhesion proteins. They are important in the formation of ADHERENS JUNCTIONS between cells. Cadherins are classified by their distinct immunological and tissue specificities, either by letters (E- for epithelial, N- for neural, and P- for placental cadherins) or by numbers (cadherin-12 or N-cadherin 2 for brain-cadherin). Cadherins promote cell adhesion via a homophilic mechanism as in the construction of tissues and of the whole animal body.
A carcinoma discovered by Dr. Margaret R. Lewis of the Wistar Institute in 1951. This tumor originated spontaneously as a carcinoma of the lung of a C57BL mouse. The tumor does not appear to be grossly hemorrhagic and the majority of the tumor tissue is a semifirm homogeneous mass. (From Cancer Chemother Rep 2 1972 Nov;(3)1:325) It is also called 3LL and LLC and is used as a transplantable malignancy.
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
An exotic species of the family CYPRINIDAE, originally from Asia, that has been introduced in North America. They are used in embryological studies and to study the effects of certain chemicals on development.
The process of moving proteins from one cellular compartment (including extracellular) to another by various sorting and transport mechanisms such as gated transport, protein translocation, and vesicular transport.
A light microscopic technique in which only a small spot is illuminated and observed at a time. An image is constructed through point-by-point scanning of the field in this manner. Light sources may be conventional or laser, and fluorescence or transmitted observations are possible.
A cell line derived from cultured tumor cells.
Interruption or suppression of the expression of a gene at transcriptional or translational levels.
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
The process of germ cell development in the male from the primordial germ cells, through SPERMATOGONIA; SPERMATOCYTES; SPERMATIDS; to the mature haploid SPERMATOZOA.
Immunologic method used for detecting or quantifying immunoreactive substances. The substance is identified by first immobilizing it by blotting onto a membrane and then tagging it with labeled antibodies.
The pattern of GENE EXPRESSION at the level of genetic transcription in a specific organism or under specific circumstances in specific cells.
Orientation of intracellular structures especially with respect to the apical and basolateral domains of the plasma membrane. Polarized cells must direct proteins from the Golgi apparatus to the appropriate domain since tight junctions prevent proteins from diffusing between the two domains.
The uptake of naked or purified DNA by CELLS, usually meaning the process as it occurs in eukaryotic cells. It is analogous to bacterial transformation (TRANSFORMATION, BACTERIAL) and both are routinely employed in GENE TRANSFER TECHNIQUES.
A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in neoplastic tissue.
Refers to animals in the period of time just after birth.
Progressive restriction of the developmental potential and increasing specialization of function that leads to the formation of specialized cells, tissues, and organs.
Methods used for detecting the amplified DNA products from the polymerase chain reaction as they accumulate instead of at the end of the reaction.
The relationships of groups of organisms as reflected by their genetic makeup.
A negative regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Highly specialized EPITHELIAL CELLS that line the HEART; BLOOD VESSELS; and lymph vessels, forming the ENDOTHELIUM. They are polygonal in shape and joined together by TIGHT JUNCTIONS. The tight junctions allow for variable permeability to specific macromolecules that are transported across the endothelial layer.
The phenotypic manifestation of a gene or genes by the processes of GENETIC TRANSCRIPTION and GENETIC TRANSLATION.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
Hybridization of a nucleic acid sample to a very large set of OLIGONUCLEOTIDE PROBES, which have been attached individually in columns and rows to a solid support, to determine a BASE SEQUENCE, or to detect variations in a gene sequence, GENE EXPRESSION, or for GENE MAPPING.
MAMMARY GLANDS in the non-human MAMMALS.
A nonparametric method of compiling LIFE TABLES or survival tables. It combines calculated probabilities of survival and estimates to allow for observations occurring beyond a measurement threshold, which are assumed to occur randomly. Time intervals are defined as ending each time an event occurs and are therefore unequal. (From Last, A Dictionary of Epidemiology, 1995)
Surface ligands, usually glycoproteins, that mediate cell-to-cell adhesion. Their functions include the assembly and interconnection of various vertebrate systems, as well as maintenance of tissue integration, wound healing, morphogenic movements, cellular migrations, and metastasis.
A positive regulatory effect on physiological processes at the molecular, cellular, or systemic level. At the molecular level, the major regulatory sites include membrane receptors, genes (GENE EXPRESSION REGULATION), mRNAs (RNA, MESSENGER), and proteins.
Recombinant proteins produced by the GENETIC TRANSLATION of fused genes formed by the combination of NUCLEIC ACID REGULATORY SEQUENCES of one or more genes with the protein coding sequences of one or more genes.
Tumors or cancer of the human BREAST.
A set of statistical methods used to group variables or observations into strongly inter-related subgroups. In epidemiology, it may be used to analyze a closely grouped series of events or cases of disease or other health-related phenomenon with well-defined distribution patterns in relation to time or place or both.
Adherence of cells to surfaces or to other cells.
The lipid- and protein-containing, selectively permeable membrane that surrounds the cytoplasm in prokaryotic and eukaryotic cells.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
A basic element found in nearly all organized tissues. It is a member of the alkaline earth family of metals with the atomic symbol Ca, atomic number 20, and atomic weight 40. Calcium is the most abundant mineral in the body and combines with phosphorus to form calcium phosphate in the bones and teeth. It is essential for the normal functioning of nerves and muscles and plays a role in blood coagulation (as factor IV) and in many enzymatic processes.
Tumors or cancer of the PANCREAS. Depending on the types of ISLET CELLS present in the tumors, various hormones can be secreted: GLUCAGON from PANCREATIC ALPHA CELLS; INSULIN from PANCREATIC BETA CELLS; and SOMATOSTATIN from the SOMATOSTATIN-SECRETING CELLS. Most are malignant except the insulin-producing tumors (INSULINOMA).
All of the processes involved in increasing CELL NUMBER including CELL DIVISION.
Laboratory tests used to evaluate how well the kidneys are working through examination of blood and urine.
Proteins prepared by recombinant DNA technology.

Transmembrane proteins in the tight junction barrier. (1/304)

Three types of transmembrane proteins have been identified within the tight junction, but it remains to be determined how they provide the molecular basis for regulating the paracellular permeability for water, solutes, and immune cells. Several of these proteins localize specifically within the continuous cell-to-cell contacts of the tight junction. One of these, occludin, is a cell adhesion molecule that has been demonstrated to influence ion and solute permeability. The claudins are a family of four-membrane spanning proteins; unexpectedly, other members of this family have already been characterized without recognizing their relationship to tight junctions. Junction adhesion molecule, the most recently identified tight junction component, is a member of the Ig superfamily and influences the paracellular transmigration of immune cells. A plaque of cytoplasmic proteins under the junction may be responsible for scaffolding the transmembrane proteins, creating a link to the perijunctional actin cytoskeleton and transducing regulatory signals that control the paracellular barrier.  (+info)

Der p 1 facilitates transepithelial allergen delivery by disruption of tight junctions. (2/304)

House dust mite (HDM) allergens are important factors in the increasing prevalence of asthma. The lung epithelium forms a barrier that allergens must cross before they can cause sensitization. However, the mechanisms involved are unknown. Here we show that the cysteine proteinase allergen Der p 1 from fecal pellets of the HDM Dermatophagoides pteronyssinus causes disruption of intercellular tight junctions (TJs), which are the principal components of the epithelial paracellular permeability barrier. In confluent airway epithelial cells, Der p 1 led to cleavage of the TJ adhesion protein occludin. Cleavage was attenuated by antipain, but not by inhibitors of serine, aspartic, or matrix metalloproteinases. Putative Der p 1 cleavage sites were found in peptides from an extracellular domain of occludin and in the TJ adhesion protein claudin-1. TJ breakdown nonspecifically increased epithelial permeability, allowing Der p 1 to cross the epithelial barrier. Thus, transepithelial movement of Der p 1 to dendritic antigen-presenting cells via the paracellular pathway may be promoted by the allergen's own proteolytic activity. These results suggest that opening of TJs by environmental proteinases may be the initial step in the development of asthma to a variety of allergens.  (+info)

Connexin-occludin chimeras containing the ZO-binding domain of occludin localize at MDCK tight junctions and NRK cell contacts. (3/304)

Occludin is a transmembrane protein of the tight junction that functions in creating both an intercellular permeability barrier and an intramembrane diffusion barrier. Creation of the barrier requires the precise localization of occludin, and a distinct family of transmembrane proteins called claudins, into continuous linear fibrils visible by freeze-fracture microscopy. Conflicting evidence exists regarding the relative importance of the transmembrane and extracellular versus the cytoplasmic domains in localizing occludin in fibrils. To specifically address whether occludin's COOH-terminal cytoplasmic domain is sufficient to target it into tight junction fibrils, we created chimeras with the transmembrane portions of connexin 32. Despite the gap junction targeting information present in their transmembrane and extracellular domains, these connexin-occludin chimeras localized within fibrils when expressed in MDCK cells, as assessed by immunofluorescence and immunogold freeze-fracture imaging. Localization of chimeras at tight junctions depends on the COOH-terminal ZO-binding domain and not on the membrane proximal domain of occludin. Furthermore, neither endogenous occludin nor claudin is required for targeting to ZO-1-containing cell-cell contacts, since in normal rat kidney fibroblasts targeting of chimeras again required only the ZO-binding domain. These results suggest an important role for cytoplasmic proteins, presumably ZO-1, ZO-2, and ZO-3, in localizing occludin in tight junction fibrils. Such a scaffolding and cytoskeletal coupling function for ZO MAGUKs is analogous to that of other members of the MAGUK family.  (+info)

Ca(2+)-independent cell-adhesion activity of claudins, a family of integral membrane proteins localized at tight junctions. (4/304)

In multicellular organisms, various compositionally distinct fluid compartments are established by epithelial and endothelial cellular sheets. For these cells to function as barriers, tight junctions (TJs) are considered to create a primary barrier for the diffusion of solutes through the paracellular pathway [1] [2] [3]. In ultrathin sections viewed under electron microscopy, TJs appear as a series of apparent fusions, involving the outer leaflets of plasma membranes of adjacent cells, to form the so-called kissing points of TJs, where the intercellular space is completely obliterated [4]. Claudins are a family of 16 proteins whose members have been identified as major integral membrane proteins localized exclusively at TJs [5] [6] [7] [8]. It remains unclear, however, whether claudins have the cell-adhesion activity that would explain the unusual intercellular adhesion at TJs. Using mouse L-fibroblast transfectants expressing various amounts of claudin-1, -2 or -3, we found that these claudins possess Ca(2+)-independent cell-adhesion activity. Using ultrathin-section electron microscopy, we observed many kissing points of TJs between adjacent transfectants. Furthermore, the cell-adhesion activity of occludin, another integral membrane protein localized at TJs [9] [10] [11], was negligible when compared with that of claudins. Thus, claudins are responsible for TJ-specific obliteration of the intercellular space.  (+info)

Clostridium perfringens enterotoxin fragment removes specific claudins from tight junction strands: Evidence for direct involvement of claudins in tight junction barrier. (5/304)

Claudins, comprising a multigene family, constitute tight junction (TJ) strands. Clostridium perfringens enterotoxin (CPE), a single approximately 35-kD polypeptide, was reported to specifically bind to claudin-3/RVP1 and claudin-4/CPE-R at its COOH-terminal half. We examined the effects of the COOH-terminal half fragment of CPE (C-CPE) on TJs in L transfectants expressing claudin-1 to -4 (C1L to C4L, respectively), and in MDCK I cells expressing claudin-1 and -4. C-CPE bound to claudin-3 and -4 with high affinity, but not to claudin-1 or -2. In the presence of C-CPE, reconstituted TJ strands in C3L cells gradually disintegrated and disappeared from their cell surface. In MDCK I cells incubated with C-CPE, claudin-4 was selectively removed from TJs with its concomitant degradation. At 4 h after incubation with C-CPE, TJ strands were disintegrated, and the number of TJ strands and the complexity of their network were markedly decreased. In good agreement with the time course of these morphological changes, the TJ barrier (TER and paracellular flux) of MDCK I cells was downregulated by C-CPE in a dose-dependent manner. These findings provided evidence for the direct involvement of claudins in the barrier functions of TJs.  (+info)

Manner of interaction of heterogeneous claudin species within and between tight junction strands. (6/304)

In tight junctions (TJs), TJ strands are associated laterally with those of adjacent cells to form paired strands to eliminate the extracellular space. Claudin-1 and -2, integral membrane proteins of TJs, reconstitute paired TJ strands when transfected into L fibroblasts. Claudins comprise a multigene family and more than two distinct claudins are coexpressed in single cells, raising the questions of whether heterogeneous claudins form heteromeric TJ strands and whether claudins interact between each of the paired strands in a heterophilic manner. To answer these questions, we cotransfected two of claudin-1, -2, and -3 into L cells, and detected their coconcentration at cell-cell borders as elaborate networks. Immunoreplica EM confirmed that distinct claudins were coincorporated into individual TJ strands. Next, two L transfectants singly expressing claudin-1, -2, or -3 were cocultured and we found that claudin-3 strands laterally associated with claudin-1 and -2 strands to form paired strands, whereas claudin-1 strands did not interact with claudin-2 strands. We concluded that distinct species of claudins can interact within and between TJ strands, except in some combinations. This mode of assembly of claudins could increase the diversity of the structure and functions of TJ strands.  (+info)

Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. (7/304)

ZO-1, ZO-2, and ZO-3, which contain three PDZ domains (PDZ1 to -3), are concentrated at tight junctions (TJs) in epithelial cells. TJ strands are mainly composed of two distinct types of four-transmembrane proteins, occludin, and claudins, between which occludin was reported to directly bind to ZO-1/ZO-2/ZO-3. However, in occludin-deficient intestinal epithelial cells, ZO-1/ZO-2/ZO-3 were still recruited to TJs. We then examined the possible interactions between ZO-1/ZO-2/ZO-3 and claudins. ZO-1, ZO-2, and ZO-3 bound to the COOH-terminal YV sequence of claudin-1 to -8 through their PDZ1 domains in vitro. Then, claudin-1 or -2 was transfected into L fibroblasts, which express ZO-1 but not ZO-2 or ZO-3. Claudin-1 and -2 were concentrated at cell-cell borders in an elaborate network pattern, to which endogenous ZO-1 was recruited. When ZO-2 or ZO-3 were further transfected, both were recruited to the claudin-based networks together with endogenous ZO-1. Detailed analyses showed that ZO-2 and ZO-3 are recruited to the claudin-based networks through PDZ2 (ZO-2 or ZO-3)/PDZ2 (endogenous ZO-1) and PDZ1 (ZO-2 or ZO-3)/COOH-terminal YV (claudins) interactions. In good agreement, PDZ1 and PDZ2 domains of ZO-1/ZO-2/ZO-3 were also recruited to claudin-based TJs, when introduced into cultured epithelial cells. The possible molecular architecture of TJ plaque structures is discussed.  (+info)

Oncogenic Raf-1 disrupts epithelial tight junctions via downregulation of occludin. (8/304)

Occludin is an integral membrane protein of the epithelial cell tight junction (TJ). Its potential role in coordinating structural and functional events of TJ formation has been suggested recently. Using a rat salivary gland epithelial cell line (Pa-4) as a model system, we have demonstrated that occludin not only is a critical component of functional TJs but also controls the phenotypic changes associated with epithelium oncogenesis. Transfection of an oncogenic Raf-1 into Pa-4 cells resulted in a complete loss of TJ function and the acquisition of a stratified phenotype that lacked cell-cell contact growth control. The expression of occludin and claudin-1 was downregulated, and the distribution patterns of ZO-1 and E-cadherin were altered. Introduction of the human occludin gene into Raf-1-activated Pa-4 cells resulted in reacquisition of a monolayer phenotype and the formation of functionally intact TJs. In addition, the presence of exogenous occludin protein led to a recovery in claudin-1 protein level, relocation of the zonula occludens 1 protein (ZO-1) to the TJ, and redistribution of E-cadherin to the lateral membrane. Furthermore, the expression of occludin inhibited anchorage-independent growth of Raf-1-activated Pa-4 cells in soft agarose. Thus, occludin may act as a pivotal signaling molecule in oncogenic Raf- 1-induced disruption of TJs, and regulates phenotypic changes associated with epithelial cell transformation.  (+info)

The tight junction protein claudin-1 (CLDN1) has been shown to be essential for hepatitis C virus (HCV) entry-the first step of viral infection. Due to the lack of neutralizing anti-CLDN1 antibodies, the role of CLDN1 in the viral entry process is poorly understood. In this study, we produced antibodies directed against the human CLDN1 extracellular loops by genetic immunization and used these antibodies to investigate the mechanistic role of CLDN1 for HCV entry in an infectious HCV cell culture system and human hepatocytes. Antibodies specific for cell surface-expressed CLDN1 specifically inhibit HCV infection in a dose-dependent manner. Antibodies specific for CLDN1, scavenger receptor B1, and CD81 show an additive neutralizing capacity compared with either agent used alone. Kinetic studies with anti-CLDN1 and anti-CD81 antibodies demonstrate that HCV interactions with both entry factors occur at a similar time in the internalization process. Anti-CLDN1 antibodies inhibit the binding of ...
HCV is a leading cause of hepatocellular carcinoma and cirrhosis all over the world. Claudins belong to family of tight junctions proteins that are responsible for establishing barriers for controlling the flow of molecules around cells. For therapeutic strategies, regulation of viral entry into the host cells holds a lot of promise. During HCV infection claudin-1 is highly expressed in liver and believed to be associated with HCV virus entry after HCV binding with or without co-receptor CD81. The claudin-1 assembly with tight junctions is regulated by post translational modifications. During claudins assembly and disassembly with tight junctions, phosphorylation is required at C-terminal tail. In cellular proteins, interplay between phosphorylation and O-β-GlcNAc modification is believed to be functional switch, but it is very difficult to monitor these functional and vibrant changes in vivo. Netphos 2.0 and Disphos 1.3 programs were used for potential phosphorylation; NetPhosK 1.0 and KinasePhos for
The ability to invade host tissues and metastasize is the major cause of cancer-related death. During tumor invasion, metastasizing cells disrupt normal cell-matrix adhesion and acquire an invasive phenotype. Claudins are adhesion proteins localized at tight junctions (TJs). Claudin-7 is a unique TJ membrane protein in that it has a stronger basolateral membrane distribution than that of apical TJs in epithelial cells. To study the basolateral function of claudin-7, claudin-7 gene silencing experiments were carried out in a lung cancer cell line using the lentivirus shRNA approach. We found that claudin-7 knockdown (KD) cells showed disrupted cell-matrix interactions. Consequently, when claudin-7 KD cells were plated on the uncoated glass surface, they were unable to attach to the glass and died the day after plating. In contrast, control cells adhered well and grew normally. Using immunofluorescent microscopy and biochemistry methods, we found that claudin-7 co-localized and ...
The results of this study show that the amount of mobile receptor and the speed at which it diffuses varies according to its location within the cell. CD81 and claudin-1 are expressed equally in the filopodia and plasma membrane, whereas SR-BI is expressed at lower levels in the filopodia compared to the plasma membrane. We show that addition of both sE2 and sE1E2 has varying affects on both the speed and mobility of CD81 and claudin-1 and that the majority of significant effects observed for claudin-1 are observed at areas of potential cell contact. Finally, we demonstrate that addition of ITX5061 affects the diffusion coefficient of CD81 and CLDN-1 and the amount of mobile SR-BI. Furthermore, the effects on SR-BI are limited to areas of cell contact or exploratory regions. In summary, we present data which we hope will further current knowledge of the activity of these receptors in relation to their role in HCV infection ...
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Blood-brain barrier (BBB) leakage plays a key role in cerebral ischemia-reperfusion injury. It is quite necessary to further explore the characteristic and mechanism of BBB leakage during stroke. We induced a focal cerebral ischemia model by transient middle cerebral artery occlusion in male rats for defining the time course of BBB permeability within 120 h following reperfusion and evaluate the specific role of tight junction (TJ) associated proteins claudin-5, occludin, and ZO-1 as well as protein kinase C delta (PKCδ) pathway in BBB leakage induced by reperfusion injury. We verified a bimodal increase in the permeability of the BBB following focal ischemia by Evans blue assay. Two peaks of BBB permeability appeared at 3 h and 72 h of reperfusion after 2 h focal ischemia, respectively. The leak at the endothelial cell was represented at the level of transmission electron microscopy. TTC staining results showed increased infarct size with time after cerebral ischemia reperfusion. The mRNA and ...
The expression of claudin-11 in benign and malignant bladder tissue and the effect of forced expression of claudin-11 on tight junction function and invasiveness of bladder cancer cells were studied. Claudin-11 expression was tested in bladder cancer cell lines (T24/83, RT 112/84 and EJ138) using reverse transcription-polymerase chain reaction (RT-PCR) and in benign and malignant bladder tissue by quantitative RT-PCR and immunohistochemistry. T24/83 cells were transfected with the pcDNA.1/NT-GFP-TOPO vector containing full-length human claudin-11 sequence. Stable-transfected cells overexpressing claudin-11 (T24Cl-11Ex), wild-type cells (T24WT) and the empty plasmid control clone (T24GFP) were compared using transurothelial resistance (TUR), in vitro adhesion, invasion and growth assays. Claudin-11 was strongly expressed in the non-invasive RT112/84 cell line compared to the invasive T24/83 and EJ138 TCC cell lines. Benign bladder tissue demonstrated equal expression of claudin-11 mRNA as ...
DESCRIPTION (provided by applicant): Disruption of the cell-cell junction with concomitant changes in the expression of junctional proteins is a hallmark of cancer metastasis and invasion. Role of adherent junction proteins have been studied extensively in cancer, however the role of tight junction proteins is less understood. Claudins are the recently identified tetraspanins, which are integral to the structure and function of tight junctions (TJs). Recent studies have shown changes in expression/cellular localization for claudins during tumorigenesis, however a cause and effect relationship has not been established. Here, we report a highly increased expression for claudin-1 in human primary colon carcinoma and metastatic tissues and cell lines derived from similar sources with relatively frequent nuclear localization. Furthermore, using genetic manipulations of claudin-1 expression in colon cancer cell lines, we demonstrate a role for claudin-1 in the regulation of epithelial to mesenchymal ...
Claudin-1 is an integral membrane protein component of tight junctions. The Snail family of transcription factors are repressors that play a central role in the epithelial-mesenchymal transition, a process that occurs during cancer progression. Snail and Slug members are direct repressors of E-cadherin and act by binding to the specific E-boxes of its proximal promoter. In the present study, we demonstrate that overexpression of Slug or Snail causes a decrease in transepithelial electrical resistance. Overexpression of Slug and Snail in MDCK (Madin-Darby canine kidney) cells down-regulated Claudin-1 at protein and mRNA levels. In addition, Snail and Slug are able to effectively repress human Claudin-1-driven reporter gene constructs containing the wild-type promoter sequence, but not those with mutations in two proximal E-box elements. We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin-1 promoter. Moreover, an inverse correlation in ...
TY - JOUR. T1 - Interendothelial claudin-5 expression depends on cerebral endothelial cell-matrix adhesion by Β 1-integrins. AU - Osada, Takashi. AU - Gu, Yu Huan. AU - Kanazawa, Masato. AU - Tsubota, Yoshiaki. AU - Hawkins, Brian T.. AU - Spatz, Maria. AU - Milner, Richard. AU - Del Zoppo, Gregory J.. PY - 2011/10. Y1 - 2011/10. N2 - The hypothesis tested by these studies states that in addition to interendothelial cell tight junction proteins, matrix adhesion by Β 1-integrin receptors expressed by endothelial cells have an important role in maintaining the cerebral microvessel permeability barrier. Primary brain endothelial cells from C57 BL/6 mice were incubated with Β 1-integrin function-blocking antibody (Ha2/5) or isotype control and the impacts on claudin-5 expression and microvessel permeability were quantified. Both flow cytometry and immunofluorescence studies demonstrated that the interendothelial claudin-5 expression by confluent endothelial cells was significantly decreased in a ...
Read Claudin-8 Expression in Renal Epithelial Cells Augments the Paracellular Barrier by Replacing Endogenous Claudin-2, The Journal of Membrane Biology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
The aim of this study was to characterize the hCMEC/D3 cell line, an in vitro model of the human Blood Brain Barrier (BBB) for the expression of brain endothelial specific claudins-3 and -12. hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture. These results support conservation of claudin-3 and -12 expression in the hCMEC/D3 cell line and make claudin-3 a potential marker for BBB characteristics in vitro.
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Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans cells in the epidermis. Our objective was to identify immunoreactive CLDN1 in human epidermal Langerhans cells and to examine the pattern of epidermal Langerhans cells in genetic human CLDN1 deficiency [neonatal ichthyosis, sclerosing cholangitis (NISCH) syndrome]. Epidermal cells from healthy human skin labelled with CLDN1-specific antibodies were analysed by confocal laser immunofluorescence microscopy and flow cytometry. Skin biopsy sections of two patients with NISCH syndrome were stained with an antibody to CD1a expressed on epidermal Langerhans cells. Epidermal Langerhans cells and a subpopulation of keratinocytes from healthy skin were positive for CLDN1. The gross number and distribution of epidermal Langerhans cells of two patients with molecularly confirmed NISCH syndrome, however, was not grossly altered. Therefore, CLDN1 is unlikely to play a critical role in ...
Claudin-4 (Clostridium perfringens enterotoxin receptor) is a tight junction protein encoded by the gene CLDN4. Expression of Claudin-4 has been associated with either poor prognosis or a more favorable diagnosis, depending on the type of cancer. Claudin-4 has been shown to distinguish adenocarcinoma from malignant mesothelioma with 99% specificity in malignant effusions (1). Claudin-4 overexpression was able to independently predict survival in a breast cancer multivariate analysis as it was associated with poor prognosis, high tumor grade and Her2 expression and was inversely correlated with estrogen receptor staining (2). In luminal breast cancer, the increase of Claudin-4 protein was correlated with the increase of tumor grade and with Ki-67, and thus demonstrated an overall shorter life survival (3). Basal-like tumors also demonstrated overexpression of Claudin-4 (4). Counter to the above breast cancer subtypes, the presence of Claudin-4 in triple negative breast cancer was a biomarker that
Objective Helicobacter pylori strains that express the oncoprotein CagA augment risk for gastric cancer. However, the precise mechanisms through which cag+ strains heighten cancer risk have not been fully delineated and model systems that recapitulate the gastric niche are critical for understanding pathogenesis. Gastroids are three-dimensional organ-like structures that provide unique opportunities to study host-H. pylori interactions in a preclinical model. We used gastroids to inform and direct in vitro studies to define mechanisms through which H. pylori modulates expression of the cancer-associated tight junction protein claudin-7.. ...
In this work, we assessed the effects of sinomenine (SN) on intestinal octreotide (OCT) absorption both in Caco-2 cell monolayers and in rats. We also investigated the molecular mechanisms of tight junction (TJ) disruption and recovery by SN-mediated changes in the claudin-1 and protein kinase C (PKC) signaling pathway. The data showed that exposure to SN resulted in a significant decrease in the expression of claudin-1, which represented TJ weakening and paracellular permeability enhancement. Then, the recovery of TJ after SN removal required an increase in claudin-1, which demonstrated the transient and reversible opening for TJ. Meanwhile, the SN-mediated translocation of PKC-α from the cytosol to the membrane was found to prove PKC activation. Finally, SN significantly improved the absolute OCT bioavailability in rats and the transport rate in Caco-2 cell monolayers. We conclude that SN has the ability to enhance intestinal OCT absorption and that these mechanisms are related at least in part to
For every experimental group, brains from at minimum 3 distinct litter had been analyzed and when compared to the in accordance NaCl handle group. qPCR approach improvement exposed that only samples must be when compared to every other which have gone through experimental treatment, mind isolation, storage, purification and evaluation preparing steps with each other. Therefore, for every DEX-treatment the according NaCl handle group was carried out at the exact same time. In addition, owing to the large complete variety of samples, but limited sample variety which could be purified at the same time, only samples from mice at the same age and identical variety of antenatal injections ended up compared to every other by using a two-tailed Student`s t-take a look at. Data are offered as the signifies ± SEM. The major tight junction molecule and mind endothelial mobile marker claudin-five was investigated originally. Triple maternal DEX remedy drastically decreased claudin-5 mRNA expression to .54 ...
Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. Chromatin immunoprecipitation (ChIP) and luciferase reporter assay showed that SCF/c-kit
immune Uncategorized PA-824, Rabbit Polyclonal to C-RAF (phospho-Thr269). The blood-epididymis barrier (BEB) is formed by epithelial tight junctions mediating selective permeability from the PA-824 epididymal epithelium. the paracellular permeability had been examined by two strategies TER and FITC-Dextran-based tracer diffusion assays. Both assays soon add up to related outcomes indicating a time-dependent disruption from the BEB differentially for the three TGF? isoforms (TGF?3>TGF?1>TGF?2) inside a TGF?-recetor-1 kinase- and Smad-dependent way. The small junction proteins claudin-1 was discovered to be decreased by the procedure with TGF?s whereas occludin had not PA-824 been affected. Epididymal epithelial cells are mainly attentive to TGF?s PA-824 through the basolateral side recommending that TGF? may impact for the epididymal epithelium through the stroma cell tradition versions the knockdown of 1 of the claudins (1 -3 -4 or -7) led to dramatically reduced transepithelial electrical level ...
Epithelial barrier dysfunction is a significant factor in many allergic diseases, including eosinophilic esophagitis (EoE). Infiltrating leukocytes and tissue adaptations increase metabolic demands and decrease oxygen availability at barrier surfaces. Understanding of how these processes impact barrier is limited, particularly in allergy. Here, we identified a regulatory axis whereby the oxygen-sensing transcription factor HIF-1α orchestrated epithelial barrier integrity, selectively controlling tight junction CLDN1 (claudin-1). Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial barrier function, as documented in 3D organotypic epithelial cultures. L2-IL5OXA mice with EoE-relevant allergic inflammation displayed localized eosinophil oxygen metabolism, tissue hypoxia, and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of esophageal epithelial-targeted stabilized ...
The primary reservoir for hepatitis C virus (HCV) replication is believed to be hepatocytes, which are highly polarized with tight junctions (TJ) separating their basolateral and apical domains. HepG2 cells develop polarity over time, resulting in the formation and remodeling of bile canalicular (BC) structures. HepG2 cells expressing CD81 provide a model system to study the effects of hepatic polarity on HCV infection. We found an inverse association between HepG2-CD81 polarization and HCV pseudoparticle entry. As HepG2 cells polarize, discrete pools of claudin-1 (CLDN1) at the TJ and basal/lateral membranes develop, consistent with the pattern of receptor staining observed in liver tissue. The TJ and nonjunctional pools of CLDN1 show an altered association with CD81 and localization in response to the PKA antagonist Rp-8-Br-cyclic AMPs (cAMPs). Rp-8-Br-cAMPs reduced CLDN1 expression at the basal membrane and inhibited HCV infection, supporting a model where the nonjunctional pools of CLDN1 ...
Findings: hCMEC/D3 cells express claudins-3 and -12. Claudin-3 is distinctly localized to the TJ whereas claudin -12 is observed in the perinuclear region and completely absent from TJs. We show that the expression of both proteins is lost in cell passage numbers where the BBB properties are no longer fully conserved. Expression and localization of claudin-3 is not modulated by simvastatin shown to improve barrier function in vitro and also recommended for routine hCMEC/D3 culture ...
Cytoplasmic expression of claudin-1 in metastatic melanoma cells correlates to increased migration, and increased secretion of MMP-2 in a PKC dependent manner, whereas claudin-1 nuclear expressi...
The C\terminal fragment of enterotoxin (C\CPE) modulates the tight junction protein claudin and disturbs the tight junctional barrier. epithelial cells (HPDEs) had been treated with C\CPE 194 and C\CPE meters19. In well\differentiated cells of the pancreatic malignancy cell collection HPAC, C\CPE 194 and C\CPE meters19 interrupted both the hurdle and fencing features without adjustments in manifestation of claudin\1 and \4, collectively with an boost of MAPK phosphorylation. C\CPE 194, but not really C\CPE meters19, improved the cytotoxicity of the anticancer brokers gemcitabine and H\1. In differentiated pancreatic malignancy cell collection PANC\1 badly, C\CPE 194, but not really C\CPE meters19, reduced claudin\4 phrase and improved MAPK activity and the cytotoxicity of the anticancer agencies. In regular HPDEs, C\CPE 194 and C\CPE meters19 reduced claudin\4 phrase and improved the MAPK activity, whereas they do not really influence the cytotoxicity of the anticancer agencies. Our results ...
View mouse Cldn15 Chr5:136966616-136975858 with: phenotypes, sequences, polymorphisms, proteins, references, function, expression
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Familial hypomagnesemia with hypercalciuria and nephrocalcinosis is an autosomal recessive tubular disorder characterized by excessive renal magnesium and calcium excretion and chronic kidney failure. This rare disease is caused by mutations in the CLDN16 and CLDN19 genes. These genes encode the tight junction proteins claudin-16 and claudin-19, respectively, which regulate the paracellular ion reabsortion in the kidney. Patients with mutations in the CLDN19 gene also present severe visual impairment. Our goals in this study were to examine the clinical characteristics of a large cohort of Spanish patients with this disorder and to identify the disease causing mutations. We included a total of 31 patients belonging to 27 unrelated families and studied renal and ocular manifestations. We then analyzed by direct DNA sequencing the coding regions of CLDN16 and CLDN19 genes in these patients. Bioinformatic tools were used to predict the consequences of mutations. Clinical evaluation showed ocular defects in
Background: Claudins are integral membrane proteins that are involved in forming cellular tight junctions. One member of the claudin family, claudin-3, has been shown to be overexpressed in breast, ovarian, and pancreatic cancer. Here we use immunohistochemistry to evaluate its expression in benign prostatic hyperplasia (BPH), prostatic intraepithelial neoplasia (PIN), normal tissue adjacent to prostatic adenocarcinoma (NAC), primary prostatic adenocarcinoma (PCa), and metastatic prostatic adenocarcinoma (Mets).Methods: Tissue microarrays were immunohistochemically stained for claudin-3, with the staining intensities subsequently quantified and statistically analyzed using a one-way ANOVA with subsequent Tukey tests for multiple comparisons or a nonparametric equivalent. Fifty-three cases of NAC, 17 cases of BPH, 35 cases of PIN, 107 cases of PCa, and 55 cases of Mets were analyzed in the microarrays.Results: PCa and Mets had the highest absolute staining for claudin-3. Both had significantly ...
Mammals are able to rapidly produce red blood cells in response to stress. The molecular pathways used in this process are important in understanding responses to anaemia in multiple biological settings. Here we characterise the novel gene Claudin 13 (Cldn13), a member of the Claudin family of tight junction proteins using RNA expression, microarray and phylogenetic analysis. We present evidence that Cldn13 appears to be co-ordinately regulated as part of a stress induced erythropoiesis pathway and is a mouse-specific gene mainly expressed in tissues associated with haematopoietic function. CLDN13 phylogenetically groups with its genomic neighbour CLDN4, a conserved tight junction protein with a putative role in epithelial to mesenchymal transition, suggesting a recent duplication event. Mechanisms of mammalian stress erythropoiesis are of importance in anaemic responses and expression microarray analyses demonstrate that Cldn13 is the most abundant Claudin in spleen from mice infected with ...
Recent investigations revealed that epithelial TJs contain charge- and size-selective pores, which control the paracellular flux of charged and uncharged solutes. The paracellular flux occurs through two distinct pathways: one high-capacity pathway with size-restrictive pores and one low-capacity pathway that is size independent, at least for substances with radii of up to 7 Å (Van Itallie et al., 2008; Watson et al., 2001). The physical basis of the low-capacity pathway is not yet completely understood, whereas the high-capacity pathway is well described. It consists of small pores with radii of ~4 Å, and is responsible for the flux of small charged and uncharged solutes. Expression of claudin-2 affects only the high-capacity pathway, by inducing an increase in pore number and a change in charge selectivity (Van Itallie et al., 2008). In a recent attempt to model the claudin-2-induced channel (Yu et al., 2009), the pore diameter, calculated from permeability to organic cations and from the ...
Congenital tufting enteropathy (CTE) is a severe autosomal recessive human diarrheal disorder with characteristic intestinal epithelial dysplasia. CTE can be caused by mutations in genes encoding EpCAM, a putative adhesion molecule, and HAI-2, a cell surface protease inhibitor. A similar phenotype occurs in mice whose intestinal epithelial cells (IECs) fail to express the tight junction-associated protein claudin-7. EpCAM stabilizes claudin-7 in IECs, and HAI-2 regulates the cell surface serine protease matriptase, a known modifier of intestinal epithelial physiology. Therefore, we hypothesized that HAI-2, matriptase, EpCAM, and claudin-7 were functionally linked. Herein we have demonstrated that active matriptase cleaves EpCAM after Arg80 and that loss of HAI-2 in IECs led to unrestrained matriptase activity and efficient cleavage of EpCAM. Cleavage of EpCAM decreased its ability to associate with claudin-7 and targeted it for internalization and lysosomal degradation in conjunction with ...
Aim of this volume is to clarify the relationship between molecular structure and function of tight junction proteins, as well as their regulation and their role in diseases. Current research may form a basis for future diagnostic and therapeutic approaches to diseases which seem to have not much in common but are characterized by defects of organ barriers, like Crohns disease, renal hypertension, inner ear deafness, and cancerous diseases. Topics include the functions of distinct tight junction proteins as barrier or channel formers for solutes and water, characteristics of the tight junction in inflammatory bowel diseases, posttranslational modifications of tight junction proteins, the relation between renal tight junction proteins and blood pressure control, and the molecular structure of claudin-claudin interactions NOTE: Annals volumes are available for sale as individual books or as a journal. For information on institutional journal subscriptions, please visit www.blackwellpublishing.com/nyas.
Occludin plays a critical role in maintaining the barrier properties of a tight junction. Thus, mutation or absence of occludin increases epithelial leakiness which is an important barrier in preventing metastasis of cancer. Loss of occludin or abnormal expression of occludin has been shown to cause increased invasion, reduced adhesion and significantly reduced tight junction function in breast cancer tissues. Furthermore, patients with metastatic disease displayed significantly lower levels of occludin suggesting that the loss of occludin and thereby loss of tight junction integrity is important in metastatic development of breast cancer.[24]. Occludin also plays an important role in the apoptosis. The C-terminus of occludin is important in receiving and transmitting cell survival signals. In standard cells, loss or disruption of occludin and other tight junction proteins leads to initiation of apoptosis through extrinsic pathways.[25] Studies involving high levels of expression of occludin in ...
GGRNA , 2020-04-07 23:23:15 , RefSeq release 60 (20130726)] RefSeq ID Version Symbol GeneID Definition NM_001101389 NM_001101389.1 CLDN25 644672 Homo sapiens claudin 25 (CLDN25), mRNA. NM_001111319 NM_001111319.1 CLDN22 53842 Homo sapiens claudin 22 (CLDN22), mRNA. NM_020982 NM_020982.3 CLDN9 9080 Homo sapiens claudin 9 (CLDN9), mRNA. NM_001001346 NM_001001346.3 CLDN20 49861 Homo sapiens claudin 20 (CLDN20), mRNA. NM_001306 NM_001306.3 CLDN3 1365 Homo sapiens claudin 3 (CLDN3), mRNA. NM_012131 NM_012131.2 CLDN17 26285 Homo sapiens claudin 17 (CLDN17), mRNA. NM_016369 NM_016369.3 CLDN18 51208 Homo sapiens claudin 18 (CLDN18), transcript variant 1, mRNA. NM_001185056 NM_001185056.1 CLDN11 5010 Homo sapiens claudin 11 (CLDN11), transcript variant 2, mRNA. NM_005602 NM_005602.5 CLDN11 5010 Homo sapiens claudin 11 (CLDN11), transcript variant 1, mRNA. NM_182848 NM_182848.3 CLDN10 9071 Homo sapiens claudin 10 (CLDN10), transcript variant a, mRNA. NM_006984 NM_006984.4 CLDN10 9071 Homo sapiens claudin ...
The tight junction regulates passage of molecules throuth the paracellular spaces. Occludin and claudins are the specific trancmembrance protains present at the tight junction and are believed to regulate the cell barrier functions. To examine the response of the tight junction to hyperosmotic solutions, Ⅰinvestigated the effects of hyperosmotic glycerol on function and protein expression of the tight junction in ECV304 cells. Cell cytotoxicity analysis showed that the high (10%) concentration of glcerol damaged 64.1% of the ECV304 cells (p<0.001), and this was confirmed morphologically. Treatment with 1%, 2% or 5% glyserol increased the paracellular permeability of fluorescein isothiocyanate (FITC) -labeled dextran by 4.7%, 18.7% and 29.4% (p<0.05), respectively. In addition, exposure to glycerol at any concentration strongly reduced the expression of occludin, whereas enpression of claudin-1 was affected very slightly. These results suggest that hyperosmotic glycerol would certainly ...
Mouse monoclonal ZO1 tight junction protein antibody [mAbcam 61357] validated for WB, IP, Flow Cyt and tested in Human. Referenced in 2 publications and 5…
Occludin is an integral membrane protein, encoded by the OCLN gene, that is located at tight junctions. Tight junctions act as a physical barrier to prevent solutes and water from passing freely through the paracellular space. Occludin is also known as BLCPMG. It is known to interact with several cytoplasmic proteins via its C terminus, while its extracellular loops are thought to be involved in the regulation of paracellular permeability and cell adhesion. When occludin is expressed in cells that lack tight junctions, it is able to induce cell adhesion. Mutations in the OCLN gene are associated with an autosomal recessive neurologic disorder known as band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).. ...
Occludin is an integral membrane protein, encoded by the OCLN gene, that is located at tight junctions. Tight junctions act as a physical barrier to prevent solutes and water from passing freely through the paracellular space. Occludin is also known as BLCPMG. It is known to interact with several cytoplasmic proteins via its C terminus, while its extracellular loops are thought to be involved in the regulation of paracellular permeability and cell adhesion. When occludin is expressed in cells that lack tight junctions, it is able to induce cell adhesion. Mutations in the OCLN gene are associated with an autosomal recessive neurologic disorder known as band-like calcification with simplified gyration and polymicrogyria (BLC-PMG).. ...
The precise regulation of intestinal epithelial TJs is crucial to maintaining barrier function between the luminal milieu and the internal environment. Recent studies have revealed an important role for Rho GTPases in regulating TJ structure/function (22, 29). In particular, TJ strand organization has been shown to be altered by constitutively active RhoA and Rac1 mutants (22) and inactivation of GTPases by C. difficile toxins is known to cause redistribution of occludin and ZO-1 from membrane microdomains or membrane rafts (32). As a result, we have further explored the mechanisms whereby paracellular permeability is influenced by this family of mediators and investigated whether the inactivation of a single GTPase (RhoA, Rac1, or Cdc42) has an effect on TJ distribution in such membrane rafts and whether TJ proteins involved in strand formation (such as claudin-1 and -2) are altered in this setting.. Using MDCK cell lines that express constitutively active or dominant-negative RhoA, Rac1, or ...
https://doi.org/10.18632/oncotarget.14974 Lianmin Zhang, Yuan Wang, Bin Zhang, Hua Zhang, Meng Zhou, Mei Wei, Qiuping Dong, Yue Xu, Zhaosong Wang, Liuwei Gao, Yanjun Qu, Bowen Shi, Jinfang Zhu,...
OBJECTIVE To evaluate the possible role of tight junction protein Occludin in nasal polyps. METHODS The expression of Claudin-1, Occludin and ZO-1 in nasal polyps (n = 20) and healthy uncinate mucosa (n = 15) were examined using immunohistochemical staining, real-time quantitative polymerase chain reaction (PCR) and Western blot analysis. The regulatory effects of proinflammatory cytokines (IFN-γ, IL-13, IL-17, TGF-β, TGF-α) on the expression of Occludin in cultured human nasal epithelial cells were investigated. RESULTS The immunohistochemical results showed that Claudin-1, Occludin and ZO-1 were detected both in the nasal polyp group and the control group. The expression sites were the cell membrane and cytoplasm of nasal mucosa epithelial cells. The mean optical density of Claudin-1, Occludin and ZO-1 were 0.187 ± 0.076,0.172 ± 0.109 and 0.098 ± 0.035 respectively in the nasal polyp group and were significantly lower than those in the control group (0.312 ± 0.101, 0.220 ± 0.069 and 0.233
Tight junctions between epithelial and endothelial cells form selective barriers and paracellular channels and regulate paracellular transport of solutes, immune cells, and drugs. More specifically, tight junctions consist of proteins that laterally interconnect neighboring cells of epithelia and endothelia. Certain proteins seal the tight junction, so that a nearly impermeable barrier develops, whereas others form channels that allow for permeation between the cells. Recent investigations have focused on tight junction proteins, belonging to the claudin family (claudins-1 to -27 in humans) and the newly defined group of TAMP (three proteins: occludin, Marvel-D2, and tricellulin). Barriers and Channels Formed by Tight Junction Proteins I showcases work in this area clustered around three major themes: the molecular properties of tight junctions, for example, the role of the claudin family of proteins and the formation of ion and charge-selective channels; the regulation of tight junction
In this study, we demonstrated (I) distinct expression patterns of five genes encoding for proteins involved in the formation of tight junctions in esophageal mucosa. In particular Claudin-1 in ERD and to lesser extent Claudin-2 was expressed at higher levels in patients with GERD. In contrast, ZO-1, ZO-2, and Occludin were not affected by the presence of GERD. (II) In general, altered gene expression of Claudin-1/-2 did not correlate with the degree of histomorphological changes in the esophageal mucosa of patients with GERD.. Tight junctions are composed of transmembrane proteins such as Occludin, 24 Claudins, several junctional adhesion molecules (JAMs) with different isoforms, E-Cadherin as well as cytosolic binding partners [43, 44]. The selection of the five genes studied was based on functional aspects. Occludin is critical for the formation of tight junctions in most tissues [45]. Claudin-1 is one of the numerous Claudins that seals intercellular space leading to higher barrier function ...
ZO-1, ZO-2, and ZO-3, which contain three PDZ domains (PDZ1 to -3), are concentrated at tight junctions (TJs) in epithelial cells. TJ strands are mainly composed of two distinct types of four-transmembrane proteins, occludin, and claudins, between which occludin was reported to directly bind to ZO-1/ZO-2/ZO-3. However, in occludin-deficient intestinal epithelial cells, ZO-1/ZO-2/ZO-3 were still recruited to TJs. We then examined the possible interactions between ZO-1/ZO-2/ZO-3 and claudins. ZO-1, ZO-2, and ZO-3 bound to the COOH-terminal YV sequence of claudin-1 to -8 through their PDZ1 domains in vitro. Then, claudin-1 or -2 was transfected into L fibroblasts, which express ZO-1 but not ZO-2 or ZO-3. Claudin-1 and -2 were concentrated at cell-cell borders in an elaborate network pattern, to which endogenous ZO-1 was recruited. When ZO-2 or ZO-3 were further transfected, both were recruited to the claudin-based networks together with endogenous ZO-1. Detailed analyses showed that ZO-2 and ZO-3 ...
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudins are small (20-27 kilodalton (kDa)) transmembrane proteins which are found in many organisms, ranging from nematodes to human beings, and are very similar in their structure, although this conservation is not observed on the genetic level. Claudins span the cellular membrane 4 times, with the N-terminal end and the C-terminal end both located in the cytoplasm, and two extracellular loops which show the highest degree of conservation. The first extracellular loop consists on average of 53 amino acids and the second one, being slightly smaller, of 24 amino acids. The N-terminal end is usually very short (4-10 amino acids), the C-terminal end varies in length ...
What happens to the paracellular barrier when the expression of tricellulin is suppressed in epithelial cells? Two independent Eph4 cell clones with suppressed tricellulin expression (KD-1 and KD-2) were established by stably expressing two distinct short interfering RNAs (Brummelkamp et al., 2002). In both clones, tricellulin protein expression was suppressed by ,95% as determined by Western blot analysis (Fig. 5 A), and immunofluorescence microscopy did not detect any tricellulin signals at tricellular contacts (Fig. 5 B). Under confluent conditions, KD-1 and KD-2 cells showed a typical cobblestone-like appearance, and there was no significant difference discerned between parental wild-type Eph4 and KD-1/2 cells with regard to the size/shape of individual cells and the distribution of cadherins (Fig. 5 B). However, when KD-1 and KD-2 cells were stained with anti-occludin mAb (Fig. 5 C) or anti-claudin-3 pAb (not depicted), tTJs as well as bTJs showed remarkable structural changes in that both ...
The tight junctions (TJ), which are located in the apical region between epithelial and endothelial cells, regulate the paracellular diffusion of ions and small molecules and play an important role in maintaining cell polarity, cell-cell integrity, and permeability. In the lung, epithelial cells are attached by TJ structures. They provide a permeable barrier and cell communication. The loss of barrier integrity, which is maintained by the expression of claudins (Cldn), results in cellular permibilization and leads to paracellular diffusion of solutes and harmful molecules. There are 27 known Cldn homologous members in mice and human. Cldn6 is mostly expressed in embryonic stem cells and associated with the programing of epithelial cells during embryo development and lung morphogenesis. In order to test the hypothesis that Cldn6 expression affects lung morphogenesis, we analyzed the expression pattern of Cldn6 during lung ontogenesis to examine cell-specific expression pattern of Cldn6 during each
Epithelial layers are integral for many physiological processes and are maintained by intercellular adhesive structures. During disease, these structures can disassemble, leading to breakdown of epithelia. TJs (tight junctions) are one type of intercellular adhesion. Loss of TJs has been linked to the pathogenesis of many diseases. The present review focuses on the role of vesicle trafficking in regulation of TJs, in particular trafficking of the TJ protein occludin. We examine how endocytosis and endosomal recycling modulate occludin localization under steady-state conditions and during stimulated TJ disassembly. ...
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Claudin, P; Andreotti, B (2006). "A scaling law for aeolian dunes on Mars, Venus, Earth, and for subaqueous ripples". Earth and ... Chang, Kenneth (October 1, 2013). "Hitting Pay Dirt on Mars". New York Times. Retrieved October 2, 2013. Meslin, P.-Y.; Forni, ... 252 (1-2): 30-44. arXiv:cond-mat/0603656. Bibcode:2006E&PSL.252...30C. doi:10.1016/j.epsl.2006.09.004. S2CID 13910286. Sullivan ... 186 (1): 60-96. Bibcode:2007Icar..186...60C. doi:10.1016/j.icarus.2006.08.019. ...
Bordiec, M.; Carpy, S.; Bourgeois, O.; Herny, C.; Massé, M.; Perret, L.; Claudin, P.; Pochat, S.; Douté, S. (1 December 2020 ... Only about 1% of Antarctic ice area can be considered to be blue-ice area, but they have attracted scientific interest due to ... Blue-ice areas make up only about 1% of the Antarctic surface ice; however, they are locally common and scattered across the ... doi:10.1111/j.1945-5100.2001.tb01918.x. Harvey, Ralph (1 January 2003). "The Origin and Significance of Antarctic Meteorites". ...
Claudin, Philippe; Andreotti, Bruno (2006). "A scaling law for aeolian dunes on Mars, Venus, Earth, and for subaqueous ripples ... 252 (1-2): 30-44. arXiv:cond-mat/0603656. doi:10.1016/j.epsl.2006.09.004. ISSN 0012-821X. Williams, S. H.; Zimbelman, J. R.; ... 1 m in amplitude. Understanding TAR formation and evolution could offer insight into the winds that created them. In turn, ... 31 (1): e18-e18. doi:10.1130/0091-7613-31.1.e18. ISSN 1943-2682. Wolfe, Stephen A.; Huntley, David J.; Ollerhead, Jeff (2006-07 ...
Claudin, A. (1894). Les Origines de l'imprimerie à Saint-Lô en Normandie [The origins of printing at Saint-Lô in Normandy] (in ... French). Paris: A. Claudin. Le Clerc, R. (1930). Histoire du Bon Sauveur de Saint-Lô [History of the Bon Sauveur of Saint-Lô] ( ... It is also chef-lieu of an arrondissement and two cantons (Saint-Lô-1 and Saint-Lô-2). The placename derives from that of a ... Messier 1 April 1771 made at the Observatory of Saint-Lô, in Rouen] (in French). Paris: Imprimerie Royale. Mourier, Guillaume ( ...
February 2018). "claudin 5". Biology Open. 7 (2): bio030494. doi:10.1242/bio.030494. PMC 5861362. PMID 29437557. Abbott NJ, ... 8 (1): 7. doi:10.1186/2045-8118-8-7. PMC 3042981. PMID 21349155. Chen Y, Imai H, Ito A, Saito N (2013). "Novel modified method ... 34 (1): 147-52. doi:10.1111/j.1471-4159.1980.tb04633.x. PMID 7452231. Raza MW, Shad A, Pedler SJ, Karamat KA (March 2005). " ... 80 (1): 32-9. doi:10.1124/mol.111.071027. PMID 21454448. S2CID 7146614. Krol S, Macrez R, Docagne F, Defer G, Laurent S, Rahman ...
Van Itallie CM, Mitic LL, Anderson JM (July 2012). "SUMOylation of claudin-2". Annals of the New York Academy of Sciences. 1258 ... 374 (Pt 1): 1-20. doi:10.1042/BJ20030407. PMC 1223585. PMID 12773095. Liu B, Gross M, ten Hoeve J, Shuai K (March 2001). "A ... 554 (1-2): 111-8. doi:10.1016/s0014-5793(03)01116-5. PMID 14596924. S2CID 23261716. Wong KA, Kim R, Christofk H, Gao J, Lawson ... 229 (1-2): 109-16. doi:10.1016/s0378-1119(99)00033-5. PMID 10095110. Betz A, Lampen N, Martinek S, Young MW, Darnell JE (August ...
Claudin-12 is a protein that in humans is encoded by the CLDN12 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN12 claudin 12". Human CLDN12 genome location and CLDN12 gene details page in the UCSC Genome Browser. Kniesel ... 2001). "claudin-18, a novel downstream target gene for the T/EBP/NKX2.1 homeodomain transcription factor, encodes lung- and ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-20 is a protein that in humans is encoded by the CLDN20 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN20 claudin 20". CS1 maint: discouraged parameter (link) Human CLDN20 genome location and CLDN20 gene details ... Hewitt KJ, Agarwal R, Morin PJ (Aug 2006). "The claudin gene family: expression in normal and neoplastic tissues". BMC Cancer. ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin 4, also known as CLDN4, is a protein which in humans is encoded by the CLDN4 gene. It belongs to the group of claudins ... "Entrez Gene: CLDN4 claudin 4". Human CLDN4 genome location and CLDN4 gene details page in the UCSC Genome Browser. Kniesel U, ... This gene encodes an integral membrane protein, which belongs to the claudin family. The protein is a component of tight ... 2003). "Claudin-4 expression decreases invasiveness and metastatic potential of pancreatic cancer". Cancer Res. 63 (19): 6265- ...
Claudin-14 is a protein that in humans is encoded by the CLDN14 gene. It belongs to a related family of proteins called ... "Entrez Gene: CLDN14 claudin 14". Baker M, Reynolds LE, Robinson SD, Lees DM, Parsons M, Elia G, et al. (2013). "Stromal ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Sticky cells, blood vessels and cancer - the paradox of Claudin-14 - Marianne Baker, Cancer Research UK Science Update blog, 14 ...
Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. It belongs to the group of claudins. Claudins, such as ... "Entrez Gene: CLDN7 claudin 7". CS1 maint: discouraged parameter (link) Human CLDN7 genome location and CLDN7 gene details page ... 2005). "Claudin-1 is a strong prognostic indicator in stage II colonic cancer: a tissue microarray study". Mod. Pathol. 18 (4 ... 2003). "Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and ...
2007). "Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus". J. Virol. 81 (22): 12465-71. doi: ... Claudin-9 is a protein that in humans is encoded by the CLDN9 gene. It belongs to the group of claudins. This gene is involved ... "Entrez Gene: CLDN9 claudin 9". Gene discovery reveals a critical protein's function in hearing Human CLDN9 genome location and ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. It belongs to the group of claudins. Tight junctions ... "Entrez Gene: CLDN1 claudin 1". Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG (Nov 2003). "Role of claudin ... The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight ... Miyamori H, Takino T, Kobayashi Y, Tokai H, Itoh Y, Seiki M, Sato H (2001). "Claudin promotes activation of pro-matrix ...
Claudin-8 is a protein that in humans is encoded by the CLDN8 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN8 claudin 8". CS1 maint: discouraged parameter (link) Human CLDN8 genome location and CLDN8 gene details page ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Morita K, Furuse M, Fujimoto K, Tsukita S (Mar 1999). "Claudin multigene family encoding four-transmembrane domain protein ...
Claudin-22 is a protein that in humans is encoded by the CLDN22 gene. It belongs to the group of claudins. GRCh38: Ensembl ... "Entrez Gene: CLDN22 claudin 22". CS1 maint: discouraged parameter (link) Human CLDN22 genome location and CLDN22 gene details ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Heiskala M, Peterson PA, Yang Y (2001). "The roles of claudin superfamily proteins in paracellular transport". Traffic. 2 (2): ...
Claudin-16 is a protein that in humans is encoded by the CLDN16 gene. It belongs to the group of claudins. Tight junctions ... The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight ... 2004). "A Novel Claudin 16 Mutation Associated with Childhood Hypercalciuria Abolishes Binding to ZO-1 and Results in Lysosomal ... "Entrez Gene: CLDN16 claudin 16". "Salmonella infection data for Cldn16". Wellcome Trust Sanger Institute. "Citrobacter ...
Jia, P.; B. Andreotti; P. Claudin (March 2017). "Giant ripples on comet 67P/Churyumov-Gerasimenko sculpted by sunset thermal ... Hayes, A.G. (1 June 2018). "Dunes across the Solar System". Science. 360 (6392): 960-961. Bibcode:2018Sci...360..960H. doi: ... 1 June 2018). "Dunes on Pluto". Science. 360 (6392): 992-997. Bibcode:2018Sci...360..992T. doi:10.1126/science.aao2975. PMID ... spaced about 0.4 to 1 km apart, that are thought to be composed of 200-300 μm diameter particles of methane ice believed to be ...
Claudin domain-containing protein 1 is a protein that in humans is encoded by the CLDND1 gene. GRCh38: Ensembl release 89: ... "Entrez Gene: CLDND1 claudin domain containing 1". Human CLDND1 genome location and CLDND1 gene details page in the UCSC Genome ... 289 (1-2): 119-29. doi:10.1016/S0378-1119(02)00507-3. PMID 12036590. Zhang QH, Ye M, Wu XY, et al. (2001). "Cloning and ...
"Claudin-1 overexpression effect on lung adenocarcinoma cell line". NCBI GEO Profiles. Retrieved 4 May 2015. Vandepoele K, Van ... Neuroblastoma breakpoint family, member 1, or NBPF1, is a protein that is encoded by the gene NBPF1 in humans. This protein is ... The NBPF1 protein is also found to be disrupted by a chromosomal translocation between chromosomes 1 and 17 with in some cases ... Additionally, the inactivation of Far upstream element-binding protein 1 causes a decrease in NBPF1, while the inactivation of ...
Claudin-5 is a protein that in humans is encoded by the CLDN5 gene. It belongs to the group of claudins. This gene encodes a ... "Entrez Gene: CLDN5 claudin 5 (transmembrane protein deleted in velocardiofacial syndrome)". Coyne CB, Gambling TM, Boucher RC, ... Kojima S, Rahner C, Peng S, Rizzolo LJ (2002). "Claudin 5 is transiently expressed during the development of the retinal ... Tsukita S, Furuse M (2002). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-11 is a protein that in humans is encoded by the CLDN11 gene. It belongs to the group of claudins and was the first ... "Entrez Gene: CLDN11 claudin 11 (oligodendrocyte transmembrane protein)". Gow A, Southwood CM, Li JS, Pariali M, Riordan GP, ... The protein encoded by this gene belongs to the claudin family of tight junction associated proteins and is a major component ... Tiwari-Woodruff SK, Buznikov AG, Vu TQ, Micevych PE, Chen K, Kornblum HI, Bronstein JM (April 2001). "OSP/claudin-11 forms a ...
Claudin 3, also known as CLDN3, is a protein which in humans is encoded by the CLDN3 gene. It is a member of the claudin ... "Entrez Gene: CLDN3 claudin 3". Coyne CB, Gambling TM, Boucher RC, Carson JL, Johnson LG (Nov 2003). "Role of claudin ... "Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium". Cancer Res ... The protein encoded by this intron-less gene, a member of the claudin family, is an integral membrane protein and a component ...
"Entrez Gene: CLDN18 claudin 18". CS1 maint: discouraged parameter (link) Niimi T, Nagashima K, Ward JM, et al. (2001). "claudin ... Claudin 18.2) is abundant in gastric tumors. Experimental antibody IMAB362 targets Claudin 18.2 to help treat gastric cancers. ... Claudin-18 is a protein that in humans is encoded by the CLDN18 gene. It belongs to the group of claudins. CLDN18 belongs to ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-19 is a protein that in humans is encoded by the CLDN19 gene. It belongs to the group of claudins. Claudin-19 has been ... "Entrez Gene: CLDN19 claudin 19". Naeem, M.; Hussain, S.; Akhtar, N. (2011). "Mutation in the Tight-Junction Gene Claudin 19 ( ... 2006). "Kidney claudin-19: localization in distal tubules and collecting ducts and dysregulation in polycystic renal disease". ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ...
Claudin-15 is a protein that in humans is encoded by the CLDN15 gene. It belongs to the group of claudins. Among its related ... "Entrez Gene: CLDN15 claudin 15". Database, GeneCards Human Gene. "CLDN15 Gene - GeneCards , CLD15 Protein , CLD15 Antibody". ... Tsukita S, Furuse M (2003). "Claudin-based barrier in simple and stratified cellular sheets". Curr. Opin. Cell Biol. 14 (5): ... Heiskala M, Peterson PA, Yang Y (2001). "The roles of claudin superfamily proteins in paracellular transport". Traffic. 2 (2): ...
Claudin-2 is a protein that in humans is encoded by the CLDN2 gene. It belongs to the group of claudins. Members of the claudin ... Claudin-2 is expressed in cation-leaky epithelia such as that of the kidney proximal tubule. Mice that are deficient in claudin ... "Entrez Gene: CLDN2 claudin 2". CS1 maint: discouraged parameter (link) Muto, S.; Hata, M.; Taniguchi, J.; Tsuruoka, S.; ... 1998). "Claudin-1 and -2: Novel Integral Membrane Proteins Localizing at Tight Junctions with No Sequence Similarity to ...
CLDND1: Claudin domain containing 1. *CPN2: Carboxypeptidase N subunit 2. *CPOX: coproporphyrinogen oxidase (coproporphyria, ... 978-1-136-84407-2. .. *^ a b c Genome Decoration Page, NCBI. Ideogram data for Homo sapience (850 bphs, Assembly GRCh38.p3). ... SFMBT1: Scm-like with four mbt domains 1. *SLC25A20: solute carrier family 25 (carnitine/acylcarnitine translocase), member 20 ... TRAK1: trafficking kinesin-binding protein 1. *TRANK1: encoding protein Tetratricopeptide repeat and ankyrin repeat containing ...
This mechanism is mediated by host claudin-3 and claudin-4 receptors, situated at the tight junctions. Clostridium enterotoxin ... "Expression of Clostridium perfringens enterotoxin receptors claudin-3 and claudin-4 in prostate cancer epithelium". Cancer ... This allows the human claudin-3,4,6,7,8 and 14 to bind but not 1,2,5, and 10. The way the protein work is it destroys the cell ... Van Itallie CM, Betts L, Smedley JG, McClane BA, Anderson JM (January 2008). "Structure of the claudin-binding domain of ...
"Tradition and Imitation in Pierre Certon's Déploration for Claudin de Sermisy". Revue de Musicologie. 85 (1): 29-62. doi: ... ISBN 978-1-5017-0486-4. Reese, Gustave (1940). Music in the Middle Ages: With an Introduction on the Music of Ancient Times. ... ISBN 978-1-137-27770-1. Wilkins, Nigel (1979). Music in the age of Chaucer. Cambridge, England: D.S. Brewer. ISBN 978-0-85991- ... ISBN 978-1-84383-016-0. Leach, Elizabeth Eva (2014). Guillaume de Machaut: Secretary, Poet, Musician. Ithaca, New York: Cornell ...
Claudin, F., Ernstson, K., Rampino, M.R., and Anguita, F. 2001. Striae, polish, imprints, rotated fractures, and related ... Ernstson, K., Claudin, F., Schüssler, U., Anguita, F. and Ernstson, T. 2001. Impact melt rocks, shock metamorphism, and ... Missing or empty ,title= (help) Ernstson, K., Claudin, F., Schüssler, U. & Hradil, K. (2002): The mid-Tertiary Azuara and ... and Claudin, F. 1994. Shock cratering of conglomeratic quartzite pebbles and the search and identification of an Azuara (Spain ...
Famous composers of these "Parisian" chansons included Claudin de Sermisy and Clément Janequin. Janequin's La guerre, written ... 1] by French historian Jules Michelet (1798-1874) in his 1855 work Histoire de France (History of France).[2] Jules Michelet ...
Claudin 5 is transiently expressed during the development of the retinal pigment epithelium. J Membr Biol 186: 81-88, 2002 ... 123(1):9-18, 2016 *↑ Berninger TA, Polkinghorne PJ, Capon MR, et al. Color vision defect. A early sign of Sorsby retinal ... insulin-like growth factor-1 (IGF-1)[23], ciliary neurotrophic factor (CNTF)[24], pigment epithelium-derived factor (PEDF)[25] ... Brian M. Kevany and Krzysztof Palczewski, Phagocytosis of Retinal Rod and Cone Photoreceptors, Physiology (Bethesda), 25(1): 8- ...
Sertoliho bunky tvoria pomocou tesných spojov (proteínmi ako je claudin alebo occludin) hemato-semenníkovú bariéru, ktorá ... 1, s. 3-11. Dostupné online [cit. 2018-08-22]. ISSN 0920-1742. DOI: 10.1007/s10695-012-9606-4. (po anglicky). ... 1, s. 27-34. Dostupné online [cit. 2018-08-22]. ISSN 0006-3363. DOI: 10.1095/biolreprod.107.063669. (po anglicky). ... 1. Dostupné online [cit. 2018-08-22]. ISSN 0006-3363. DOI: 10.1095/biolreprod.113.110197. (po anglicky). ...
Claudin 1, which is a tight-junction protein, and CD81 link to create a complex, priming them for later HCV infection processes ... Claudin-1, and Occludin.[29][30] The envelope of HCV is similar to very low-density lipoproteins (VLDL) and low-density ... 1 (4): 1276-1286. doi:10.2741/e458. PMID 22201953.. *^ a b c Halegoua-De Marzio, Dina; Fenkel, Jonathan (January 27, 2014). " ... 2010: 1-12. doi:10.1155/2010/548280. PMC 2896694. PMID 20625493.. *^ Rauch, A.; Gaudieri, S.; Thio, C.; Bochud, P. Y. (2009). " ...
Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane ... vermutlich Claudin bei CPE,[14] sowie vermutlich GPI-Anker oder andere Glykosylierungen beim ε-Toxin. Durch die ... Nummer 1, Januar 2013, S. 73-84, ISSN 1746-0921. doi:10.2217/fmb.12.131. PMID 23252494. PMC 3570152 (freier Volltext). ... 1] Im Gegensatz zu porenbildenden Toxinen sind Bakteriocine oder die nichtribosomalen Peptide wie z. B. Polymyxine oder Iturine ...
Phantom of the Opera (1943) - Erique Claudin/The Phantom of the Opera ... Ova stranica je zadnji put izmijenjena 15:58, 1 juli 2016.. *Tekst je dostupan pod Creative Commons Attribution/Share-Alike ...
142 (1): 359-69. doi:10.1210/en.142.1.359. PMID 11145599.. *^ Kelley, Robert O.; Vogel, Kathryn G.; Crissman, Harry A.; Lujan, ... 6 (1): 1-12. doi:10.1007/BF01175410. PMID 839246. S2CID 30090247.. *^ Shibata, Y; Yamamoto, T (March 1977). "Gap junctions in ... 34 (1): 39-54. doi:10.1007/BF01870292. PMID 561191. S2CID 2831462.. *^ Corsaro CM, Migeon BR (October 1977). "Comparison of ... 9 (1): 117-28. PMID 9550065.. *^ Cusato, K; Bosco, A; Rozental, R; Guimarães, CA; Reese, BE; Linden, R; Spray, DC (July 2003). ...
Anatole Claudin (1898). The First Paris Press: an account of the books printed for G. Fichet and J. Heynlin in the Sorbonne, ... 1971- [1]. *. Henri-Jean Martin; Roger Chartier, eds. (1989-1991). Histoire de l'édition française (in French) (2nd ed.). Paris ... 1-2. Brill. ISBN 9789004156876. .. + Volumes 3-4 (2011): Books published in France before 1601 in Latin and Languages other ...
Cates, M. E.; Wittmer, J. P.; Bouchaud, J.-P.; Claudin, P. (31 August 1998). "Jamming, Force Chains, and Fragile Matter". ... He is the 19th Lucasian Professor of Mathematics at the University of Cambridge and has held this position since 1 July 2015. ...
These complexes, formed primarily of members of the claudin and the occludin families, consist of about 35 different proteins, ... 124 (1): 3-22. doi:10.1016/j.jaci.2009.05.038. ISSN 0091-6749. PMC 4266989. PMID 19560575. Bennett, M. V.; Barrio, L. C.; ... 92 (1-2): 256-262. doi:10.1016/j.physbeh.2007.05.017. ISSN 0031-9384. PMID 17582445. Cario, E (2010). "Heads up! How the ... Geibel, John P. (1 January 2005). "Secretion and absorption by colonic crypts". Annual Review of Physiology. 67: 471-490. doi: ...
... is concentrated at tight junctions through its possible interaction with claudin-1 and junctional adhesion molecule". The ... 1 (3): 287-92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614. Ebnet K, Suzuki A, Horikoshi Y, Hirose T, Meyer Zu ... 277 (1): 455-61. doi:10.1074/jbc.M109005200. PMID 11689568. KDR+protein,+human at the US National Library of Medicine Medical ... 310 ( Pt 1) (1): 155-62. doi:10.1042/bj3100155. PMC 1135867. PMID 7646439. "Entrez Gene: F11R F11 receptor". Ebnet K, Schulz CU ...
... has overexpressed HER2/neu Normal breast-like Claudin-low: a more recently described class; often triple-negative, but distinct ... 1 point: tubular formation in more than 75% of the tumor (it may in addition be termed "majority of tumor") 2 points: tubular ... 1 point: nuclei with minimal or mild variation in size and shape 2 points: nuclei with moderate variation in size and shape 3 ... 13 (1): 2-7. doi:10.2325/jbcs.13.2. PMID 16518056. Normanno N, De Luca A, Carotenuto P, Lamura L, Oliva I, D'Alessio A (2009 ...
Claudin E is a molecule found in tight junctions that appears to be expressed in the EVL and required for normal zebrafish ... "The tight junction component claudin E is required for zebra fish epiboly". Developmental Dynamics. 239 (2): 715-722. doi: ... 124 (1): 269-80. doi:10.1016/0012-1606(87)90478-7. PMID 3666309. C.B. Kimmel; R.M. Warga; T.F. Schilling (1990-04-01). "Origin ... 83 (1-2): 77-94. doi:10.1016/S0925-4773(99)00036-2. PMID 10381569. Conway G, Margoliath A, Wong-Madden S, Roberts RJ, Gilbert W ...
Markov AG, Falchuk EL, Kruglova NM, Radloff J, Amasheh S (January 2016). "Claudin expression in follicle-associated epithelium ... 216 (1): 13-4. doi:10.1111/apha.12595. PMID 26335934. Pascall, C R; Stearn, E J; Mosley, J G (1980-07-05), "Short Reports", ... 216 (1): 112-9. doi:10.1111/apha.12559. hdl:11701/6438. PMID 26228735. S2CID 13389571. Lelouard H, Fallet M, de Bovis B, ...
The name claudin comes from Latin word claudere ("to close"), suggesting the barrier role of these proteins. A recent review ... All human claudins (with the exception of Claudin 12) have domains that let them bind to PDZ domains of scaffold proteins. ... "Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin". J. ... "A systems proteomics view of the endogenous human claudin protein family". J Proteome Res. 15 (2): 339-359. doi:10.1021/acs. ...
Claudin de Sermisy, Thomas Crecquillon, Domenico Ferrabosco, Jean de Latre, Jacquet de Berchem, Jakob Meiland, Alexander ... 300 R/Vv, 123". The codex, consisting of 183 folia, is bound in parchment with the initials "P W S P" and the number "1 5 9 1" ... ISBN 83-900905-1-1. Erdman, Jerzy (1997). Tabulatura Gdańska - The Gdańsk Tablature - 1591 (CD Recording). Polskie Nagrania. ... Jahrhunderts, Hänssler Verlag 1988 (Edition features pieces 1 - 40 and presents them partially with a pedal part on three ...
"Systems Proteomics View of the Endogenous Human Claudin Protein Family". Journal of Proteome Research. 15 (2): 339-359. doi: ... 13 (1): 15-20. doi:10.1021/pr401144x. PMC 3928647. PMID 24364385. Liu, S; Im, H; Bairoch, A; Cristofanilli, M; Chen, R; Deutsch ... 12 (1): 45-57. doi:10.1021/pr300985j. PMC 4142220. PMID 23259914. "The Global Proteome Machine and Database". Retrieved 2014-09 ...
"Decreased lactate dehydrogenase B expression enhances claudin 1-mediated hepatoma cell invasiveness via mitochondrial defects ... 505 (1): 33-41. doi:10.1016/j.abb.2010.10.010. PMID 20951115. Zha X, Wang F, Wang Y, He S, Jing Y, Wu X, Zhang H (2011). " ... 71 (1): 13-8. doi:10.1158/0008-5472.CAN-10-1668. PMID 21199794. Kim JH, Kim EL, Lee YK, Park CB, Kim BW, Wang HJ, Yoon CH, Lee ... 117B (1): 11-7. doi:10.1002/ajmg.b.10015. PMID 12555229. S2CID 25994539. Mazzotta S, Guerranti R, Gozzetti A, Bucalossi A, ...
MicroRNA Gong Y, Renigunta V, Himmerkus N, Zhang J, Renigunta A, Bleich M, Hou J (April 2012). "Claudin-14 regulates renal Ca ... 7 (1): e29837. Bibcode:2012PLoSO...729837M. doi:10.1371/journal.pone.0029837. PMC 3254631. PMID 22253797. Chang SJ, Weng SL, ...
Claudin de Sermisy, French composer (b. 1495) October 18 - Anne d'Alençon, French noblewoman (b. 1492) November 7 - Maldeo ... March 1 - Over 80 (?) Huguenots are massacred by the ultra-Catholic Francis, Duke of Guise in Wassy-sur-Blaise, marking the ... May 1 - Jean Ribault, French navigator, lands in Florida, and later establishes a Huguenot colony at Charlesfort on Parris ... July 1 - Wilhelm IV of Eberstein, German President of the Reichskammergericht (b. 1497) July 4 - Johann Hommel, German ...
1382/1383-1395) José Serebrier (born 1938) Claudin de Sermisy (c. 1490 - 1562) Kazimierz Serocki (1922-1981) Alexander Serov ( ... Retrieved 1 September 2020. CS1 maint: discouraged parameter (link) (subscription or UK public library membership required) ... Retrieved 1 September 2020. CS1 maint: discouraged parameter (link) (subscription or UK public library membership required) ... Retrieved 1 September 2020. CS1 maint: discouraged parameter (link) (subscription or UK public library membership required) ...
Nevertheless, another sources document existence of mTEC unipotent progenitors that express claudin 3 and 4 (Cld3/4). These two ... "Medullary thymic epithelial cells expressing Aire represent a unique lineage derived from cells expressing claudin". Nature ... 140 (1): 123-35. doi:10.1016/j.cell.2009.12.030. PMID 20085707. Guha M, Saare M, Maslovskaja J, Kisand K, Liiv I, Haljasorg U, ... 5 (1): 166-79. doi:10.1016/j.celrep.2013.08.038. PMC 3820422. PMID 24095736. Gordon J, Wilson VA, Blair NF, Sheridan J, Farley ...
ISBN 978-84-7222-878-8. Azcárate, Manuel; Fernando Claudin (1979). Marc Abeles; Charles-Albert Ryng (eds.). L'Europe de ... ISBN 978-1-78076-108-4. Retrieved 2015-11-29. Eaton, Samuel D. (1981). The Forces of Freedom in Spain 1974-1979: A Personal ... ISBN 978-1-4128-1999-2. Retrieved 2015-11-29. Bolloten, Burnett (1991). The Spanish Civil War: Revolution and Counterrevolution ... ISBN 1-134-55408-7. Retrieved 2015-11-29. Sánchez Cervelló, Josep; Agudo, Sebastián (2015-04-23). Las Brigadas Internacionales ...
Claudin de Sermisy set the psalm. In the 17th century, Henry Dumont set this psalm for La Chapelle Royale au Louvre (1666). In ... Verse 1 is the final verse of Nishmat. Verses 2, 10, and 13 are recited during Selichot. Verses 10, 13, and 14 are part of the ... Verse 17 is quoted in Mary's song of praise, the Magnificat, in Luke 1:50. In the Western church, this psalm was traditionally ... Verses 1-5: The Psalmist's personal experience of God's compassion; Verses 6-19: The attributes of God as seen in his ...
While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the ... Often, several claudin family members are coexpressed and interact with each other, and this determines the overall ... IPR006187. Claudin. IPR003548. Claudin1. IPR017974. Claudin_CS. IPR004031. PMP22/EMP/MP20/Claudin. ... IPR006187. Claudin. IPR003548. Claudin1. IPR017974. Claudin_CS. IPR004031. PMP22/EMP/MP20/Claudin. ...
Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed ... Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed ... Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). Claudin-1 is expressed ... Claudin-1 Target. Polyclonal Antibody - Claudin-1 Antibody - Immunoprecipitation, Western Blotting, UniProt ID O95832, Entrez ...
Using FRET analysis, the proximity of claudin N- and C-termini integrated in homopolymeric strands composed of claudin-3 or of ... The results indicate at least two different cis-interaction interfaces within claudin-3 homopolymers as well as within claudin- ... FRET; cell junctions; claudin; claudin strand model; live-cell imaging; tight junction architecture ... Human claudin-1 and claudin-3, fused to ECFP or EYFP at the N- or C-terminus, were expressed in the TJ-free cell line HEK ( ...
... and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of ... claudin-1). Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial ... α/claudin-1 axis regulates barrier dysfunction in eosinophilic esophagitis. ... α/claudin-1 axis regulates barrier dysfunction in eosinophilic esophagitis. ...
Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice. J Cell ... Heatmaps generated from normalized data (n = 3). (E) Western blotting confirmed claudin-1 protein loss in HIF1A-KD and HIF1A-DN ... We first noted that normal human esophageal biopsies possess the primary claudin genes CLDN1, CLDN4, and CLDN7 (Figure 3C). We ... B) Mice were assessed for claudin-1 protein by Western blot, quantified by densitometry, and CLDN1 mRNA by real-time RT-PCR. (C ...
... the presence of claudin 1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to ... the presence of claudin 1 in the Golgi cisterns may indicate the presence of tight junction precursors before transportation to ... and RAs stained positive with anti-claudin 1 antibodies. Since it has been shown that ameloblasts repeatedly alternate between ... and RAs stained positive with anti-claudin 1 antibodies. Since it has been shown that ameloblasts repeatedly alternate between ...
2018 Dec 15;373(1-2):44-56. doi: 10.1016/j.yexcr.2018.08.012. Epub 2018 Aug 9. Research Support, Non-U.S. Govt ... TUSC3 accelerates cancer growth and induces epithelial-mesenchymal transition by upregulating claudin-1 in non-small-cell lung ... On the contrary, overexpression of TUSC3 significantly enhanced EMT progress by upregulating claudin-1 expression. Overall, our ... In addition, TUSC3 knockdown suppressed epithelial-mesenchymal transition by downregulating the expression of claudin-1, which ...
Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression ... In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant ... Furthermore, decreased expression of claudin-3 was obtained in the colonic epithelium from the c-Kit loss-of-function mutant ... We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon ...
... a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Tight junctions are… ... Recombinant protein of human claudin 1 (CLDN1). Human. > 80 % Preparation: Recombint protein was captured through anti-DDK ... Background of Claudin-1 / CLDN1 antibody. Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and ... Western blot (WB) analysis of Claudin-1 antibody (Cat.-No.: AP06060PU-N) at 1/500 dilution Lane1:Hela whole cell lysate Lane2: ...
Claudin-1 / CLDN1, 50 µg. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets ... Claudin-1 / CLDN1. Not available. Human > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column ... Positive controls for Claudin-1 / CLDN1 (3 products). Catalog No.. Species. Pres.. Purity. Source. ... Proteins for Claudin-1 / CLDN1 (3 products). Catalog No.. Species. Pres.. Purity. Source. ...
GST-claudin-6 tail, GST-claudin-9 tail, GST-claudin-1 tail(YV/AA), GST-claudin-6 tail(YV/AA), or GST-claudin-9 tail(YV/AA). ... Claudin-1 was transported from the ER in an in vitro budding assay. Huh7.5 cells were treated with digitonin to prepare the ... C) Cells surface claudin-1 was quantified by FACS assay in nonpermeabilized cells. Huh7.5 cells treated with 1.562 μg/ml XN or ... Claudin-1 utilizes the YV motif-Sec24C interaction to exit from the ER to the Golgi compartment and eventually reach its final ...
In this study, claudin 1 promotes migration in luminal-like MCF7 human breast cancer cells and increases their sensitivity to ... Claudin 1 promotes migration and increases sensitivity to tamoxifen and anticancer drugs in luminal-like human breast cancer ... Downregulation of claudin 1, a critical tight junction protein, has been correlated with increased invasiveness in breast ... Collectively, our results suggest that claudin 1 has the potential to be used as a predictive marker for treatment efficacy for ...
A Claudin-1-Targeted HTA. Mayo Yamashita, Manami Iida, Minoru Tada, Yoshitaka Shirasago, Masayoshi Fukasawa, Shotaro Nagase, ... A Claudin-1-Targeted HTA. Mayo Yamashita, Manami Iida, Minoru Tada, Yoshitaka Shirasago, Masayoshi Fukasawa, Shotaro Nagase, ... Discovery of Anti-Claudin-1 Antibodies as Candidate Therapeutics against Hepatitis C Virus. Mayo Yamashita, Manami Iida, Minoru ... Claudin-1 (CLDN1), a known host factor for hepatitis C virus (HCV) entry and cell-to-cell transmission, is a target molecule ...
Claudin 1 (8G1) Monoclonal Antibody. Catalog Number. Pack Size. List Price*. Quantity. ... Aqueous buffered solution containing 1xTBS (pH7.4), 1%BSA, 40%Glycerol and 0.05% Sodium Azide. Store at -20°C for 12 months.. ...
... occludin and claudin-5 rearrangement and lessened IgG and Evans blue extravasation. (A) Occludin, ZO-1 and claudin-5 expression ... claudin-5 was continuously located along ECs, and fewer gaps were formed in the metformin-treated group (Figure 4A). Gap ... claudin-5 was continuously located along ECs, and fewer gaps were formed in the metformin-treated group (Figure 4A). Gap ... occludin and claudin-5 rearrangement and lessened IgG and Evans blue extravasation. (A) Occludin, ZO-1 and claudin-5 expression ...
Claudin 1 antibody LS-C331975 is an unconjugated rabbit polyclonal antibody to Claudin 1 (CLDN1) from human, mouse and rat. ... Claudin 1 antibody LS-C331975 is an unconjugated rabbit polyclonal antibody to Claudin 1 (CLDN1) from human, mouse and rat. ... Claudin 1 antibody LS-C331975 is an unconjugated rabbit polyclonal antibody to Claudin 1 (CLDN1) from human, mouse and rat. ... Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human CLDN1 (NP_066924.1). ...
... claudin 4, and claudin 7 in colorectal cancer and its relation with CLDN DNA methylation patterns. Hahn-Strömberg, Victoria ... Altered claudin expression has been described in colon, prostatic, ovarian, and breast carcinoma. However, the role of ... Tight junction, methylation, colon cancer, claudin Nationell ämneskategori Cancer och onkologi Identifikatorer. URN: urn:nbn:se ... We aimed our study to investigate whether claudin protein expression and methylation of CLDN can influence the tumorigenesis of ...
Claudin 7 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunohistochemistry (IHC), ... Claudin 7 Antibody 1 Publications. Rabbit Polyclonal, Catalog number: 10118-1-AP ... Claudin 7 antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9). ... Claudin 7 antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9). ...
Claudin 18 antibody Mouse Monoclonal from Proteintech validated in Immunoprecipitation (IP), Immunohistochemistry (IHC), Enzyme ... Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight ... Claudin 18 Antibody 1 Publications. Mouse Monoclonal, Catalog number: 66167-1-Ig ,CloneNo.: 5G7F2 ... IP Result of anti-Claudin 18 (IP:66167-1-Ig, 3ug; Detection:66167-1-Ig 1:300) with mouse stomach tissue lysate 4000ug. ...
We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin-1 promoter. ... The transcription factors Slug and Snail act as repressors of Claudin-1 expression in epithelial cells1. Ofelia M. Martínez- ... Moreover, an inverse correlation in the levels of Claudin-1 and Slug transcripts were observed in breast cancer cell lines. E- ... Claudin-1 is an integral membrane protein component of tight junctions. The Snail family of transcription factors are ...
Rabbit Anti-Claudin 1 Polyclonal Antibody, Purified Rabbit Polyclonal Antibody (Pab) validated in WB, IF, IHC-P (AP52052), ... home , Products , Primary Antibodies , Rabbit Anti-Claudin 1 Polyclonal Antibody Rabbit Anti-Claudin 1 Polyclonal Antibody. ... While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the ... Often, several claudin family members are coexpressed and interact with each other, and this determines the overall ...
Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans ... Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans ... Download PDF Human epidermal Langerhans cells express the tight junction protein claudin-1 and are present in human genetic ... Human epidermal Langerhans cells express the tight junction protein claudin-1 and are present in human genetic claudin-1 ...
Effects of claudin-2, occludin or ZO-1 siRNA transfection on transepithelial electrical resistance (TER) in HK-2 cell ... claudin -2 expressiontubule epithelial cell line HK -2.claudin -2ZORNAHK -2 cellscontrol HK -2 monolayersTJ protein expression ... claudin -2 expressiontubule epithelial cell line HK -2.claudin -2ZORNAHK -2 cellscontrol HK -2 monolayersTJ protein expression ... Effects of claudin-2, occludin or ZO-1 siRNA transfection on transepithelial electrical resistance (TER) in HK-2 cell ...
CLAUDIN-1, EXPRESSION, CD81, RECEPTOR, IN-VIVO, CELL ENTRY, HUMAN LIVER, ENTRY FACTORS, HUMAN HEPATOCYTES, NEUTRALIZING ... Using a human liver-chimeric mouse model(6), we show that a monoclonal antibody specific for the TJ protein claudin-1 (ref. 7) ... Using a human liver-chimeric mouse model(6), we show that a monoclonal antibody specific for the TJ protein claudin-1 (ref. 7) ... Clearance of persistent hepatitis C virus infection in humanized mice using a claudin-1-targeting monoclonal antibody. Laurent ...
a) Immunohistochemistry of claudin-1 in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy ... b) Immunoblotting of claudin-1 in the rat vagina. The lower panels denote the means ± standard error of 6 experiments for each ... b) Immunoblotting of claudin-1 in the rat vagina. The lower panels denote the means ± standard error of 6 experiments for each ... Claudin-1 was most intense in the superficial layer of the vaginal epithelium in the control group. Expression of ZO-1, ...
We further provide direct evidence for the binding of the NFκB subunit p65 to the GLI1 promoter in both EMT and claudin-low ... We observed non-canonical activation of GLI1 in claudin-low and EMT cell lines, and identified crosstalk with the NFκB pathway ... Knockdown of GLI1 expression in claudin-low cell lines resulted in reduced cell viability, motility, clonogenicity, self- ... High GLI1 expression is a property of claudin-low cells and tumors and correlates with markers of EMT and breast cancer stem ...
The gene encoding claudin-1 (CLDN1) has been mapped to human chromosome 3 (HSA3; 3q28→q29) using a radiation hybrid panel. ... The gene encoding claudin-1 (CLDN1) has been mapped to human chromosome 3 (HSA3; 3q28→q29) using a radiation hybrid panel. ... Schempp, W und Schmid, M (2005) Comparative mapping of human claudin-1 (CLDN1) in great apes. Cytogenetic and Genome Research ...
... even though claudin 4 did not significantly correlate with patient age. Claudin 1 knockdown in BT-20 cells resulted in ... Although the expression of one of these proteins, claudin 1, is down regulated in most invasive human breast cancers (HBC), we ... In these tumors, the claudin 1 protein, usually localized in the cell membrane, is often mislocalized to the cytoplasm. To ... The association of high claudin 1 protein levels observed in tumors derived from older women with BLBC, suggests that claudin 1 ...
During HCV infection claudin-1 is highly expressed in liver and believed to be associated with HCV virus entry after HCV ... The claudin-1 assembly with tight junctions is regulated by post translational modifications. During claudins assembly and ... and Thr 191 may provide an on/off switch to regulate assembly of claudin-1 at tight junctions. In addition these ... Claudin-4 is phosphorylated on Ser-194, Thr-189, claudin-3 at Thr-192, claudin-16 at Ser-217, claudin-5 at Thr-205 and Thr-207 ...
IntroductionThe recently identified claudin-low subtype of breast cancer is enriched for cells with stem-like and mesenchymal- ... We further provide direct evidence for the binding of the NF¿B subunit p65 to the GLI1 promoter in both EMT and claudin-low ... IntroductionThe recently identified claudin-low subtype of breast cancer is enriched for cells with stem-like and mesenchymal- ... We observed non-canonical activation of GLI1 in claudin-low and EMT cell lines, and identified crosstalk with the NF¿B pathway. ...
  • Tight junctions are composed of claudin and occludin proteins, which join the junctions to the cytoskeleton (1,2). (cellsignal.com)
  • The claudin family is composed of 23 integral membrane proteins, and their expression, which varies among tissue types, may determine both the strength and properties of the epithelial barrier. (cellsignal.com)
  • Probing the cis-arrangement of prototype tight junction proteins claudin-1 and claudin-3. (nih.gov)
  • Cell lysates from Huh7.5.1 cells were incubated with purified GST-claudin-1 tail or GST, and proteins were pulled down with glutathione-Sepharose beads. (asm.org)
  • Candidate claudin-1 tail-interacting proteins were the GST-claudin-1-tail interactome. (asm.org)
  • Claudin-low breast cancers are characterized by low expression levels of cell-cell adhesion molecules including E-cadherin and several of the tight junction claudin proteins, claudin 3, 4, and 7. (biomedcentral.com)
  • Although the expression of one of these proteins, claudin 1, is down regulated in most invasive human breast cancers (HBC), we have recently shown that high levels of claudin 1, characterized tumors belonging to the very aggressive basal-like breast cancer (BLBC) subtype. (biomedcentral.com)
  • As well, the exact combination of claudin proteins within a given tissue determines the selectivity, strength and tightness of the TJ [ 11 ]. (biomedcentral.com)
  • Several classes of claudin interact with other proteins to form tight junction and regulate permeability of TJs. (biomedcentral.com)
  • It is also observed that the expression of claudin proteins found to be differentially regulated in several cancers. (biomedcentral.com)
  • The transmembrane domain proteins of the claudin superfamily are the major structural components of cellular tight junctions. (chalmers.se)
  • In MC3T3-E1 cells but not MLO-Y4 cells, mRNAs of claudin-1, -2, and -6, Cx43, and type I collagen, and proteins of claudin-1 and Cx43 were increased after treatment with IGF-I. Such treatment significantly decreased paracellular permeability in MC3T3-E1 cells. (elsevier.com)
  • Specific role of tight junction proteins claudin-5, occludin, and ZO-1 of the blood-brain barrier in a focal cerebral ischemic insult. (qxmd.com)
  • This study was performed to determine whether BK opens the BTB by affecting the tight junction (TJ)-associated proteins zonula occluden-1 (ZO-1), occludin, and caludin-5 and cytoskeleton protein filamentous actin (F-actin). (qxmd.com)
  • Most of the claudins (claudin-12 being the exception) have a C-terminal PDZ-binding motif that can interact with other PDZ domain proteins, such as scaffolding protein, ZO-1, -2 and -3 [ PMID: 24665401 ]. (ebi.ac.uk)
  • The hypothesis tested by these studies states that in addition to interendothelial cell tight junction proteins, matrix adhesion by Β 1-integrin receptors expressed by endothelial cells have an important role in maintaining the cerebral microvessel permeability barrier. (elsevier.com)
  • Much to our surprise, however, we have now also discovered several major TJ proteins (claudins 1 and 4, occludin, cingulin, symplekin, protein ZO-1) and TJ-related structures in specific positions of formations of epithelium-derived tissues that lack any lumen and do not border on luminal or body surfaces. (nih.gov)
  • Hepatitis C virus (HCV) entry is dependent on host cell molecules tetraspanin CD81, scavenger receptor BI and tight junction proteins claudin-1 and occludin. (ox.ac.uk)
  • Recent investigations have focused on tight junction proteins, belonging to the claudin family (claudins-1 to -27 in humans) and the newly defined group of TAMP (three proteins: occludin, Marvel-D2, and tricellulin). (wiley.com)
  • Recent evidence indicates that both exposure to HIV-1 and HIV-1 specific proteins, such as Tat protein, can contribute to alterations of expression and distribution of claudin-5 in brain endothelial cells and brain microvessels. (elsevier.com)
  • The expressions of ZO-1 and claudin-5 proteins were determined by immunofluorescence staining and Western Blot . (bvsalud.org)
  • The results of immunofluorescence double staining showed that ZO-1 and claudin-5 proteins could not only coexist in the same cell , but also could be expressed separately in different cells . (bvsalud.org)
  • Linear regression analysis showed the positive correlation between the expressions of ZO-1 and claudin-5 proteins in brain tissue of rats with acute CO poisoning (R2= 0.917, P = 0.022). (bvsalud.org)
  • Conclusion NBP could markedly improve the ultrastructure and functional integrity of BBB through up-regulating the expressions of ZO-1 and claudin-5 proteins , and then reduce brain damage caused by CO poisoning . (bvsalud.org)
  • Recent work points toward the claudin family of tight junction proteins as leading candidates for the molecular components that regulate paracellular permeability properties in epithelial tissues. (uab.edu)
  • Our results show that claudin-6 protein is significantly down-regulated in breast invasive ductal carcinomas and is an important correlate with lymphatic metastasis, but claudin-6 down-regulation was not correlated with upregulation of the methylation associated proteins (MeCP2, DNMT1) or histone modification associated proteins (HDAC1, H3Ac, H4Ac). (biomedcentral.com)
  • These proteins, such as claudin-5, occludin, and zona occludens 1 (ZO-1) prevent transcellular diffusion of molecules and cells ( 1 ). (frontiersin.org)
  • Other tight junction proteins, zonula occludens (ZO)-1, ZO-2, claudin-2, and claudin-3, were also decreased in polyamine-deficient cells. (physiology.org)
  • Major transmembrane and cytosolic tight junction proteins in the mammalian epithelium include occludin, claudins, zonula occludens (ZO)-1, and ZO-2 ( 8 , 12 , 41 , 54 , 66 ). (physiology.org)
  • To this end, MDCK C7 cells were stably transfected with either claudin-2 or claudin-10b, two paracellular cation-channel-forming TJ proteins that are not endogenously expressed in this cell line. (biologists.org)
  • Perturbation was overexpression of either claudin-2 or claudin-10b, as both form cation channels within the TJ of MDCK C7 cells, a tight renal epithelial cell line that lacks endogenous expression of these TJ proteins. (biologists.org)
  • Molecular architecture of tight junction has been studied extensively, which has confirmed that claudin family of proteins is integral component of tight junction. (hindawi.com)
  • however, the role of claudin family of proteins play in a series of pathophysiological events, including human carcinoma development, is only now beginning to be understood. (hindawi.com)
  • Several transmembrane adhesive proteins have been identified which include vascular endothelial (VE) and neural (N) cadherin at adherens junctions (AJs), 6,7 occludin, 8 and members of the claudin family, 9 as well as the junctional adhesion molecule (JAM) family at tight junctions (TJs). (ahajournals.org)
  • 10,11 PECAM-1 12,13 or S-endo-1/Muc 18/CD146 14 are proteins that are not components of the major junctional systems but localize along the intercellular cleft. (ahajournals.org)
  • Gh, prolactin and cortisol on mRNA expression of three tight junction proteins were examined: claudin-10e (Cldn-10e), Cldn-30 and zonula occludens-1 (Zo-1). (genscript.com)
  • Claudin 7 belongs to a family of 24 tight junction (TJ) membrane proteins which are central for the regulation of cell permeability and the maintenance of cell polarity. (perkinelmer.com)
  • A closer examination of isolated tight junctions uncovered two related ~22 kDa, four-transmembrane domain proteins, claudin-1 and claudin-2, with no similarity to occludin. (thermofisher.com)
  • Excitement in the tight junction field continues to rise following the recent discovery of claudins -3, -4, -5, -6, -7, and -8 and experiments suggesting that tight junctions in different tissues are comprised of different sets of claudin family proteins. (thermofisher.com)
  • Here, we identified a regulatory axis whereby the oxygen-sensing transcription factor HIF-1α orchestrated epithelial barrier integrity, selectively controlling tight junction CLDN1 (claudin-1). (jci.org)
  • Claudin1 (CLDN1), a member of the claudin family, is an integral membrane protein and a component of tight junction strands. (acris-antibodies.com)
  • Different tissues exhibit different Claudin composition and CLDN1 expression is often cell type and tissue dependent. (acris-antibodies.com)
  • Western Blot analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M01), clone 1C5-D9.Lane 1: CLDN1 transfected lysate(23 KDa).Lane 2: Non-transfected lysate. (acris-antibodies.com)
  • WB Suggested Anti-CLDN1 Antibody Titration: 0.2-1 ug/ml. (acris-antibodies.com)
  • Western Blot analysis of CLDN1 expression in transfected 293T cell line ( H00009076-T02 ) by CLDN1 MaxPab polyclonal antibody.Lane 1: CLDN1 transfected lysate(22.70 KDa).Lane 2: Non-transfected lysate. (acris-antibodies.com)
  • Claudin 1 antibody LS-C331975 is an unconjugated rabbit polyclonal antibody to Claudin 1 (CLDN1) from human, mouse and rat. (lsbio.com)
  • Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human CLDN1 (NP_066924.1). (lsbio.com)
  • In conclusion, our results show that CLDN1 is significantly hypomethylated in tumor samples and that the membrane staining intensity for claudin 1, 4, and 7 is significantly lower in colorectal cancer tissues than in adjacent nonneoplastic tissue. (diva-portal.org)
  • The nucleotide sequence data reported for Canis familiaris partial mRNA for putative claudin-1 protein ( cldn1 gene) has been submitted to DDBJ, EMBL, GenBank® and GSDB Nucleotide Sequence Databases under the accession number AJ628857 . (biochemj.org)
  • Claudin-1 (CLDN1) is a structural tight junction (TJ) protein and is expressed in differentiating keratinocytes and Langerhans cells in the epidermis. (uzh.ch)
  • The tight junction protein claudin-1 (CLDN1) has been shown to be required for entry of HCV into the cell. (ox.ac.uk)
  • Three host cell molecules have been reported to be important entry factors or receptors for HCV internalization: scavenger receptor BI, the tetraspanin CD81, and the tight junction protein claudin-1 (CLDN1). (ox.ac.uk)
  • The tight junction protein claudin-1 (CLDN1) has been shown to be essential for hepatitis C virus (HCV) entry-the first step of viral infection. (bham.ac.uk)
  • In this study, we show that CLDN1 has a causal role in the epithelial-mesenchymal transition (EMT) in human liver cells, and that the c-Abl-Ras-Raf-1-ERK1/2 signaling axis is critical for the induction of malignant progression by CLDN1. (ajou.ac.kr)
  • Collectively, these results indicate that CLDN1 is necessary for the induction of EMT in human liver cells, and that activation of the c-Abl-Ras-Raf-1-ERK1/2 signaling pathway is required for CLDN1-induced acquisition of the malignant phenotype. (ajou.ac.kr)
  • The main results are that (i) within homo- and heteropolymers, the average distance between the cytoplasmic ends of the TM1s of cis-interacting claudin molecules is shorter than the average distance between their TM4s, and (ii) TM1 segments of neighbouring claudins are oriented towards each other as the cytoplasmic end of TM1 is in close proximity to more other TM1 segments than TM4 is to other TM4 segments. (nih.gov)
  • In normal mucosa, cytoplasm showed no staining for claudins in any patient, whereas in paired colorectal cancer tissues, significant cytoplasmic staining appeared both for claudin 1 (p = 0.04) and claudin 4 (p = 0.01). (diva-portal.org)
  • Claudins are the backbone of tight junction strands, and are essential components to the function of tight junctions [1]. (oatext.com)
  • It is through those "other" functions [1,2] that TJs and especially claudins play a role in carcinogenesis. (oatext.com)
  • A prime example of the aforementioned role of claudins in carcinogenesis and metastases is claudin-1. (oatext.com)
  • Claudin-1 along with claudin-3 and claudin-5 are shown to promote pro-MMP2 whereby claudins recruit MMPs on the cell surface to achieve elevated focal concentrations and eventual activations of pro-MMP2 [12]. (oatext.com)
  • The claudin-19/C-CPE complex shows no density of a short extracellular helix that is critical for claudins to assemble into TJ strands. (rcsb.org)
  • However, there is little knowledge about the relationship of ASK1 and claudins, especially claudin-6. (biomedcentral.com)
  • In humans, 24 claudins (claudin 1-24) have been identified. (ebi.ac.uk)
  • Several claudin mouse knockout models have been generated and the diversity of phenotypes observed clearly demonstrates their important roles in the maintenance of tissue integrity in various organs and suggest that claudins also participate in cellular contexts other than tight junctions. (hindawi.com)
  • Claudin-4 is the first to confer ionic selectivity to paracellular transport, leading to the prediction that the combination of different claudins defines the overall selectivity of different junctions. (thermofisher.com)
  • C) Cell lysates from Huh7.5.1 cells were pulled down by GST-CD81-N-tail, GST-CD81-C-tail, GST-occludin-tail, GST-SRB1-tail, GST-claudin-1 tail or GST with two extra washes. (asm.org)
  • Metformin promoted ZO-1, occludin and claudin-5 rearrangement and lessened IgG and Evans blue extravasation. (nih.gov)
  • A) Occludin, ZO-1 and claudin-5 expression in sham group, NS and metformin-treated mice at 3 days following tMCAO. (nih.gov)
  • B) Representative result of occludin, ZO-1 and claudin-5 expression at 1 and 3 days after tMCAO. (nih.gov)
  • Bar graphs show a quantification of occludin, ZO-1 and claudin-5 expression. (nih.gov)
  • To evaluate endothelial cell permeability after metformin treatment, we conducted occludin/CD31, ZO-1/CD31 and claudin-5/CD31 double staining to observe tight junction distribution in situ at 3 days after tMCAO. (nih.gov)
  • Result indicated that occludin and ZO-1 were continuously located on the margin of ECs in sham group, claudin-5 was continuously located along ECs, and fewer gaps were formed in the metformin-treated group (Figure 4A). (nih.gov)
  • Effects of claudin-2, occludin or ZO-1 siRNA transfection on transepithelial electrical resistance (TER) in HK-2 cell monolayers. (figshare.com)
  • TER was significantly decreased by claudin-2 siRNA transfection, but significantly increased by siRNA transfection against occludin or ZO-1. (figshare.com)
  • The objectives of this study were to investigate the localization of tight junctions and the modulation of zonula occludens- (ZO-) 1, occludin and claudin-1 expression by estrogen in castrated female rat vagina. (nih.gov)
  • The cellular localization and expression of ZO-1, occludin, and claudin-1 were determined in each group by immunohistochemistry and western blot. (nih.gov)
  • Expression of ZO-1, occludin, and claudin-1 was significantly decreased after ovariectomy and was restored to the level of the control after estrogen replacement. (nih.gov)
  • In order to investigate the regulation of expression of tight-junction molecules and of function during bone differentiation, osteoblast-like MC3T3-E1 cells and osteocyte-like MLO-Y4 cells were treated with IGF-I. In both MC3T3-E1 cells and MLO-Y4 cells, the tight-junction molecules occludin, claudin-1, -2, and -6, and the gap-junction molecule connexin 43 (Cx43) were detected by reverse transcription with polymerase chain reaction. (elsevier.com)
  • This same OGD treatment also led to rapid degradation of tight junction protein occludin and dissociation of claudin-5 from the cytoskeleton, which contributed to OGD-induced endothelial barrier disruption. (qxmd.com)
  • Using selective MMP-2/9 inhibitor SB-3CT (2-[[(4-phenoxyphenyl)sulfonyl]methyl]-thiirane) or their neutralizing antibodies or Cav-1 siRNA, we found that MMP-2 was the major enzyme mediating OGD-induced occludin degradation, while Cav-1 was responsible for claudin-5 redistribution. (qxmd.com)
  • Moreover, occludin protein loss and claudin-5 redistribution were detected in ischemic cerebromicrovessels. (qxmd.com)
  • These data indicate that cerebral ischemia initiates two rapid parallel processes, MMP-2-mediated occludin degradation and Cav-1-mediated claudin-5 redistribution, to cause BBB disruption at early stroke stages relevant to acute thrombolysis. (qxmd.com)
  • In rat brain glioma model and BTB model in vitro, we find that the protein expression levels of ZO-1, occludin, and claudin-5 are attenuated by BK induction. (qxmd.com)
  • The redistribution of ZO-1, occludin, and claudin-5 and rearrangement of F-actin in brain microvascular endothelial cells are observed at the same time. (qxmd.com)
  • This study demonstrates that the increase of BK-mediated BTB permeability is associated with the down-regulation of ZO-1, occludin, and claudin-5 and the rearrangement of F-actin and that cAMP/PKA signal transduction system might be involved in the modulating process. (qxmd.com)
  • FAK is an integrated component of the blood-testis barrier (BTB) involved in regulating Sertoli cell adhesion via its effects on the occludin-zonula occludens-1 complex. (pnas.org)
  • Recent studies have shown that FAK is a regulatory protein that modulates TJ integrity at the BTB via its effects on the phosphorylation status of the adhesion protein complexes, such as the occludin-zonula occludens (ZO)-1 complex ( 6 ). (pnas.org)
  • In contrast to occludin, which induces only a small number of short strands at cell-cell contact sites when introduced into fibroblasts lacking tight junctions, claudin-1 and -2 induce networks of strands characteristic of true tight junctions. (thermofisher.com)
  • Though inconclusive, these findings suggest that claudin-1 and -2 are major structural components of TJ strands and that occludin plays some other accessory role. (thermofisher.com)
  • Unexpectedly, the anti-claudin-1 antibody localized claudin to the Golgi apparatus of a sub-population of SA and RA in addition to the distal junctions of RA. (frontiersin.org)
  • Immunohistochemistry (IHC) analyzes of Claudin-1 antibody (Cat. (acris-antibodies.com)
  • Western blot (WB) analysis of Claudin-1 antibody (Cat. (acris-antibodies.com)
  • Final antibody concentration is 1 mg/ml. (acris-antibodies.com)
  • The lysates were immunoprecipitated with anti-claudin-1 antibody followed by Western blotting. (asm.org)
  • Claudin 1 (8G1) Monoclonal Antibody by Bioss, Cat. (lucerna-chem.ch)
  • Using a human liver-chimeric mouse model(6), we show that a monoclonal antibody specific for the TJ protein claudin-1 (ref. 7) eliminates chronic HCV infection without detectable toxicity. (ugent.be)
  • Mice with PU.1 deficiency in T cells were protected from colitis, whereas treatment with antibody to IL-9 suppressed colitis. (nature.com)
  • Primary brain endothelial cells from C57 BL/6 mice were incubated with Β 1-integrin function-blocking antibody (Ha2/5) or isotype control and the impacts on claudin-5 expression and microvessel permeability were quantified. (elsevier.com)
  • Claudin-9 Polyclonal antibody specifically detects Claudin-9 in Human samples. (fishersci.com)
  • We offer Claudin-14 Antibody Pairs for use in common research applications: Immunoprecipitation, Western Blot. (novusbio.com)
  • Each Claudin-14 Antibody Pair is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. (novusbio.com)
  • Our Claudin-14 Antibody Pairs can be used in a variety of model species: Human. (novusbio.com)
  • Choose from our Claudin-14 Antibody Pairs. (novusbio.com)
  • The following product was used in this experiment: Claudin 5 Monoclonal Antibody (4C3C2) from Thermo Fisher Scientific, catalog # 35-2500, RRID AB_2533200. (thermofisher.com)
  • Recent antibody-based studies indicated that Claudin 7 is not only localized at the apical TJs but also have a strong basolateral membrane distribution in the epithelia of various tissues. (perkinelmer.com)
  • This antibody reacts specifically with the ~ 22-24 kDa endogenous Claudin-5 protein. (thermofisher.com)
  • Distal junction area of ruffle-ended ameloblasts (RA) and the Golgi apparatus of a sub-population of smooth-ended ameloblasts (SA) and RAs stained positive with anti-claudin-1 antibodies. (frontiersin.org)
  • B) Huh7.5 cells treated with Sec24C siRNA or a control siRNA for 72 h were stained with antibodies against claudin-1 (green) and the tight junction marker ZO-1 (red). (asm.org)
  • A) Huh7.5 cells treated with different concentrations of XN for 12 h were fixed with methanol and stained with antibodies against claudin-1 (red) and the tight junction marker ZO-1 (green). (asm.org)
  • Both yeast membrane-bound and detergent-extracted, purified claudin-1 were antigenic and recognized by specific antibodies. (chalmers.se)
  • Monoclonal anti-claudin 1 antibodies prevent hepatitis C virus infection of primary human hepatocytes. (ox.ac.uk)
  • Inhibition of hepatitis C virus infection by anti-claudin-1 antibodies is mediated by neutralization of E2-CD81-claudin-1 associations. (bham.ac.uk)
  • While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. (uniprot.org)
  • Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. (uniprot.org)
  • These findings suggest that the induction of tight-junction protein claudin-1 and paracellular permeability during the differentiation of osteoblast-like MC3T3-E1 cells after treatment with IGF-I is regulated via a MAP-kinase pathway, but not with respect to gap junctions. (elsevier.com)
  • Exposure of bEND3 monolayer to OGD for 2 h significantly increased its permeability to FITC-labeled dextran and promoted the secretion of metalloproteinase-2 and -9 (MMP-2/9) and cytosolic translocation of caveolin-1 (Cav-1). (qxmd.com)
  • Caveolin-1 regulates nitric oxide-mediated matrix metalloproteinases activity and blood-brain barrier permeability in focal cerebral ischemia and reperfusion injury. (qxmd.com)
  • The protoplasmic or exoplasmic face association of tight junction particles cannot predict paracellular permeability or heterotypic claudin compatibility. (ebi.ac.uk)
  • This study demonstrates that blockade of Β 1-integrin function changes interendothelial claudin-5 expression and increases microvessel permeability. (elsevier.com)
  • Hence, endothelial cell-matrix interactions via Β 1-integrin directly affect interendothelial cell tight junction claudin-5 expression and brain microvascular permeability. (elsevier.com)
  • BBB permeability can be altered by several factors including inflammatory molecules such as interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), C-C motif chemokine receptor-2 ligand (CCL-2), and interleukin-17A (IL-17A) ( 1 ). (frontiersin.org)
  • IL-1β contributes to BBB permeability in three major ways. (frontiersin.org)
  • Second, IL-1β induces expression of hypoxia-inducible factor-1α (HIF-1α) and VEGF-A, contributing to BBB permeability and increased angiogenesis ( 4 ). (frontiersin.org)
  • Third, secreted IL-1β also alters the location of CXCL12 expression in CNS endothelial cells from the basolateral BBB membrane to the luminal surface, contributing to BBB permeability to leukocytes ( 6 ). (frontiersin.org)
  • Under all conditions, water flux in claudin-2-transfected cells was elevated compared with vector controls, indicating claudin-2-mediated paracellular water permeability. (biologists.org)
  • These results suggest that protease-mediated epithelial PAR-1 and -2 activation, by migrating PMNs, induces signaling events that increase epithelial permeability thereby facilitates PMN transepithelial migration. (jimmunol.org)
  • In addition, Snail and Slug are able to effectively repress human Claudin - 1 -driven reporter gene constructs containing the wild-type promoter sequence, but not those with mutations in two proximal E-box elements. (biochemj.org)
  • Taken together, these results support the hypothesis that Claudin - 1 is a direct downstream target gene of Snail family factors in epithelial cells. (biochemj.org)
  • However, the effects of microRNA-1303 (miR-1303) on gastric cancer (GC) cells and the upstream regulation of GC-associated claudin-18 gene (CLDN18) remain unclear. (springer.com)
  • The claudin gene family: expression in normal and neoplastic tissues. (springer.com)
  • In our previous study, we found that claudin-6 is preferentially expressed in mammary epithelial cells and functions as a potential breast cancer suppressor gene [ 20 ], which is supported by the follow-up study of Osanai [ 21 ]. (biomedcentral.com)
  • To study the basolateral function of claudin-7, claudin-7 gene silencing experiments were carried out in a lung cancer cell line using the lentivirus shRNA approach. (aacrjournals.org)
  • We also generated claudin-7-deficient (Cldn7 −/− ) mice using targeted-gene deletion method. (aacrjournals.org)
  • Claudin-6 is a candidate tumor suppressor gene in breast cancer, and has been shown to be regulated by DNA methylation and histone modification in breast cancer lines. (biomedcentral.com)
  • This gene encodes a member of the claudin family. (thermofisher.com)
  • The gene expression characteristics of claudin-low-like MPA/DMBA-induced tumors accurately reflected those of human claudin-low tumors, including epithelial-mesenchymal transition phenotype, high level of immune activation, and low degree of differentiation. (uio.no)
  • CLDN16 (Claudin 16) is a Protein Coding gene. (genecards.org)
  • Immunohistochemical staining was used to study protein expression in both tumor and the adjacent nonneoplastic mucosa of claudin 1, 4, and 7. (diva-portal.org)
  • Knockdown of GLI1 expression in claudin-low cell lines resulted in reduced cell viability, motility, clonogenicity, self-renewal, and reduced tumor growth of orthotopic xenografts. (biomedcentral.com)
  • Interestingly, no significant association was found between claudin 1 and nodal involvement, tumor grade or tumor size. (biomedcentral.com)
  • miR-1303 was significantly overexpressed whereas claudin-18 was downregulated in GC tissues and cell lines, which was significantly associated with tumor size, location invasion, histologic type and tumor-node-metastasis stage. (springer.com)
  • Endoglin tumor cell expression was associated with E-cadherin and β-catenin expression (p=0.001 and p=0.068), but not with claudin-1 expression (p=0.299). (ejmjih.com)
  • A particular characteristic of EMT is the downregulation of E-cadherin expression, which causes disruption of cell-cell junctions and dissemination of cells from the primary tumor ( 1 ). (aacrjournals.org)
  • In mice, the medroxyprogesterone acetate (MPA) and 7,12-dimethylbenzanthracene (DMBA)-induced mammary tumor model yields a heterogeneous set of tumors, a subset of which display claudin-low features. (uio.no)
  • article{6717062, abstract = {Hepatitis C virus (HCV) infection is a leading cause of liver cirrhosis and cancer(1). (ugent.be)
  • a) Immunohistochemistry of claudin-1 in vaginal tissue from animals of the control (Con), ovariectomy (Ovx), and ovariectomy plus 17β-estradiol treatment (Ovx + Est) groups. (nih.gov)
  • This study analysed the expression of claudin-1 by immunohistochemistry in 99 tissue samples (including 33 colorectal cancer cases with 33 concordant lymph node metastases and 33 concordant cancer-adjacent normal tissues, all from the same patients). (oatext.com)
  • We have investigated the expression of claudin-6, MeCP2, HDAC1, H3Ac and H4Ac in 100 breast invasive ductal carcinoma tissues and 22 mammary gland fibroadenoma tissues using immunohistochemistry. (biomedcentral.com)
  • On the contrary, overexpression of TUSC3 significantly enhanced EMT progress by upregulating claudin-1 expression. (nih.gov)
  • Overexpression of Slug and Snail in MDCK (Madin-Darby canine kidney) cells down-regulated Claudin-1 at protein and mRNA levels. (biochemj.org)
  • Taken together, our results indicate that overexpression of claudin-2 promotes self-renewal within CRC stem-like cells, suggesting a potential role for this protein as a therapeutic target in CRC. (aacrjournals.org)
  • Overexpression studies suggest that tight junction leakiness in these two strains of MDCK cells is conferred by expression of the tight junction protein claudin-2. (uab.edu)
  • Recent studies have shown high claudin-3 levels in several solid tumors, but the regulation mechanism of claudin-3 expression remains poorly understood. (mdpi.com)
  • High GLI1 expression is a property of claudin-low cells and tumors and correlates with markers of EMT and breast cancer stem cells. (biomedcentral.com)
  • Claudin-low tumors are mainly triple-negative invasive ductal carcinomas with a high frequency of metaplastic and medullary differentiation. (biomedcentral.com)
  • In these tumors, the claudin 1 protein, usually localized in the cell membrane, is often mislocalized to the cytoplasm. (biomedcentral.com)
  • The association of high claudin 1 protein levels observed in tumors derived from older women with BLBC, suggests that claudin 1 has the potential to serve as a marker which can identify a specific subgroup of patients within the BLBC subtype and thus, further contribute to the characterization of these ill-defined breast cancers. (biomedcentral.com)
  • Furthermore, using genetic manipulations of claudin-1 expression in colon cancer cell lines, we demonstrate a role for claudin-1 in the regulation of epithelial to mesenchymal transition (EMT), growth of xenografted tumors and metastasis in athymic mice. (elsevier.com)
  • Here we report that elevated claudin-2 expression in stage II/III colorectal tumors is associated with poor recurrence-free survival following 5-FU-based chemotherapy, an outcome in which CSC play an instrumental role. (aacrjournals.org)
  • Results: Forty-six (66.7%), 67 (97.1%), and 67 (97.1%) of the tumors, expressed immunostaining for claudin-1, E-cadherin and β-catenin, respectively. (ejmjih.com)
  • Neither the genomic characteristics of MPA/DMBA-induced claudin-low tumors nor those of human claudin-low breast tumors have been thoroughly explored. (uio.no)
  • A publicly available dataset was queried to explore the genomic characteristics of human claudin-low breast cancer and to validate findings in the murine tumors. (uio.no)
  • Half of MPA/DMBA-induced tumors showed a claudin-low-like subtype. (uio.no)
  • however, none accurately delineated claudin-low-like tumors. (uio.no)
  • There was an elevated expression of the immunosuppressive genes PTGS2 (encoding COX-2) and CD274 (encoding PD-L1) in human and murine claudin-low tumors. (uio.no)
  • Downregulation of claudin 1, a critical tight junction protein, has been correlated with increased invasiveness in breast cancer. (sigmaaldrich.com)
  • B ) HIF-1α expression was assessed by Western blot of nuclear protein lysate from esophageal epithelial cells exposed to a time course (0, 4, 24, 48, 72 hours) of experimental hypoxia compared with duration-matched normoxic cells and quantified by densitometry. (jci.org)
  • Prolonged experimental hypoxia or HIF1A knockdown suppressed HIF-1α-dependent claudin-1 expression and epithelial barrier function, as documented in 3D organotypic epithelial cultures. (jci.org)
  • In conclusion, SCF/c-kit-JNK/AP-1 signaling pathway significantly promoted claudin-3 expression in colonic epithelium and CRC, which could contribute to epithelial barrier function maintenance and to CRC development. (mdpi.com)
  • Claudin-5 (CLDN-5), a tetra-transmembrane protein is a key component of the TJ seal that prevents the paracellular diffusion of drugs into the CNS. (aspetjournals.org)
  • These data show for the first time that the ERK 1/2 signaling pathway negatively controls claudin-2 expression in mammalian renal epithelial cells and provide evidence for regulation of tight junction paracellular transport by alterations in claudin composition within tight junction complexes. (uab.edu)
  • We conclude that claudin-2, but not claudin-10b, forms a paracellular water channel and thus mediates paracellular water transport in leaky epithelia. (biologists.org)
  • In the present study, colorectal cancer (CRC) tissues, HT-29 and DLD-1 CRC cell lines, CRC murine model (C57BL/6 mice) and c-kit loss-of-function mutant mice were used. (mdpi.com)
  • We demonstrated that elevated claudin-3 levels were positively correlated with highly expressed c-kit in CRC tissues based upon analysis of protein expression. (mdpi.com)
  • The expression of miR-1303 and claudin-18 in GC tissues and gastric cancer cell lines were detected by qRT-PCR and western blotting, respectively. (springer.com)
  • Here, we report a highly increased expression for claudin-1 in human primary colon carcinoma and metastatic tissues and cell lines derived from similar sources with relatively frequent nuclear localization. (elsevier.com)
  • Claudin-1 is expressed primarily in keratinocytes (4) and normal mammary epithelial cells, but is absent or reduced in breast carcinomas and breast cancer cell lines (5,6). (cellsignal.com)
  • TUSC3 accelerates cancer growth and induces epithelial-mesenchymal transition by upregulating claudin-1 in non-small-cell lung cancer cells. (nih.gov)
  • In vitro, claudin-3 expression was clearly increased in CRC cells by overexpressed c-kit or stimulated by exogenous recombinant human stem cell factor (rhSCF), while significantly decreased by the treatment with c-kit or c-Jun N-terminal kinase (JNK) inhibitors. (mdpi.com)
  • Cell lysates from Huh7.5.1 cells were pulled down by GST-claudin-1 tail or GST followed by Western blotting. (asm.org)
  • D and E) Immunoblots showing the association of Sec24C with claudin-1 in transfected HEK293T cells by a coimmunoprecipitation assay. (asm.org)
  • HEK293T cells were transfected with Flag-claudin-1, Flag-SRB1, or PCMV-Tag2 empty vector for 48 h. (asm.org)
  • F) Coimmunoprecipitation of endogenous claudin-1 with Sec24C in Huh7.5.1 cells. (asm.org)
  • G) Colocalization of claudin-1 with Sec24C in Huh7.5.1 cells. (asm.org)
  • Huh7.5.1 cells were fixed by PFA and immunofluorescently labeled for claudin-1 (green) and Sec24C (red). (asm.org)
  • C) Cell surface claudin-1 was quantified by FACS assay in nonpermeabilized cells. (asm.org)
  • D) Intercellular claudin-1 was quantified by FACS assay in permeabilized cells. (asm.org)
  • Claudin 1 promotes migration and increases sensitivity to tamoxifen and anticancer drugs in luminal-like human breast cancer cells MCF7. (sigmaaldrich.com)
  • Furthermore, metformin activated AMPK signaling pathway and alleviated oxygen-glucose deprivation-induced ICAM-1 expression in bEND.3 cells (P (nih.gov)
  • For claudin 4, the percentage of cells staining positively was also significantly reduced (p = 0.04). (diva-portal.org)
  • The recently identified claudin-low subtype of breast cancer is enriched for cells with stem-like and mesenchymal-like characteristics. (biomedcentral.com)
  • Claudin 1 knockdown in BT-20 cells resulted in decreased cell migration. (biomedcentral.com)
  • We observed non-canonical activation of GLI1 in claudin-low and EMT cell lines, and identified crosstalk with the NF¿B pathway.ConclusionsThis work highlights the importance of GLI1 in the maintenance of characteristics of metastatic breast cancer stem cells. (diagenode.com)
  • Here we found more T cells expressing the transcription factor PU.1 and interleukin 9 (IL-9) in patients with ulcerative colitis. (nature.com)
  • The expression of claudin-1 in MC3T3-E1 cells after IGF-I treatment was mainly upregulated via a mitogen-activated protein (MAP)-kinase pathway and, in part, modulated by a PI3-kinase pathway, whereas Cx43 expression and the mediated gap-junctional intercellular communication protein did not contribute to the upregulation. (elsevier.com)
  • Furthermore, in MC3T3-E1 cells during wound healing, upregulation of claudin-1 was observed together with an increase of IGF-I and type I collagen. (elsevier.com)
  • Previous studies have demonstrated that claudin-6 functions as a cancer suppressor in human MCF-7 breast cancer cells. (biomedcentral.com)
  • In patient-derived organoids, primary cells, and cell lines, claudin-2 promoted CRC self-renewal in vitro and in multiple mouse xenograft models. (aacrjournals.org)
  • Next-generation sequencing in ALDHHigh cells revealed that claudin-2 regulated expression of nine microRNAs known to control stem cell signaling. (aacrjournals.org)
  • Both flow cytometry and immunofluorescence studies demonstrated that the interendothelial claudin-5 expression by confluent endothelial cells was significantly decreased in a time-dependent manner by Ha2/5 exposure relative to isotype. (elsevier.com)
  • Claudin-7 is a unique TJ membrane protein in that it has a stronger basolateral membrane distribution than that of apical TJs in epithelial cells. (aacrjournals.org)
  • We found that claudin-7 knockdown (KD) cells showed disrupted cell-matrix interactions. (aacrjournals.org)
  • Consequently, when claudin-7 KD cells were plated on the uncoated glass surface, they were unable to attach to the glass and died the day after plating. (aacrjournals.org)
  • This cell-matrix defect can be rescued by transfecting claudin-7 or integrin β1 back to the claudin-7 KD cells. (aacrjournals.org)
  • In the seminiferous epithelium, the blood-testis barrier (BTB) created by coexisting tight junction (TJ) and basal ectoplasmic specialization [basal ES, a testis-specific adherens junction (AJ)] between Sertoli cells near the basement membrane is one of the tightest blood-tissue barriers in the mammalian body ( 1 ). (pnas.org)
  • Results The ultrastructure of BBB was normal in normal control group , some ZO-1 and a large number of claudin-5 positive cells were observed. (bvsalud.org)
  • Extracellular signal-regulated kinase (ERK) 1/2 activation by hepatocyte growth factor treatment of MDCK strain II cells inhibited claudin-2 expression and transiently increased TER. (uab.edu)
  • Transfection of constitutively active mitogen-activated protein kinase/extracellular signal-regulated kinase kinase into MDCK strain II cells also inhibited claudin-2 expression and increased TER. (uab.edu)
  • MDCK strain I cells have higher levels of active ERK 1/2 than do MDCK strain II cells. (uab.edu)
  • U0126 treatment of MDCK strain I cells decreased active ERK 1/2 levels, induced expression of claudin-2 protein, and decreased TER by ∼20-fold. (uab.edu)
  • AJOU Open Repository: Claudin-1 induces epithelial-mesenchymal transition through activation of the c-Abl-ERK signaling pathway in human liver cells. (ajou.ac.kr)
  • Because TJs of basal cells are primarily composed of claudin-11, claudin-11-deficient (Cld11 -/- ) mice were generated with an expectation that the compartmentalization in stria vascularis in these mice would be affected. (biologists.org)
  • 1-5 In general, signal transduction by junctional components is sustained and directed to establish homeostasis of the cells. (ahajournals.org)
  • For example, VE-cadherin, claudin-5, or PECAM-1 have only been found in endothelial but not in epithelial cells. (ahajournals.org)
  • The present invention relates to novel peptides derived from Claudin-6 (CLDN6), complexes comprising such peptides bound to recombinant MHC molecules, and cells presenting said peptide in complex with MHC molecules. (wipo.int)
  • In addition, TUSC3 knockdown suppressed epithelial-mesenchymal transition by downregulating the expression of claudin-1, which plays an indispensable role in EMT progress. (nih.gov)
  • We also interrogated the outcome of claudin 1 knockdown in a human BLBC cell line, BT-20. (biomedcentral.com)
  • Knockdown of claudin-7 by lentivirus claudin-7 shRNA downregulated integrin β1 at both mRNA and protein levels. (aacrjournals.org)
  • Lastly, epithelial PAR-1 and -2 knockdown decreased the rate of PMN transepithelial migration. (jimmunol.org)
  • These data demonstrate that medullary NaCl loading induces ET-1 and P2-independent natriuresis in intact females. (bireme.br)
  • Alteration in claudin protein expression pattern is associated with several types of cancer (2,3). (cellsignal.com)
  • L2-IL5OXA mice with EoE-relevant allergic inflammation displayed localized eosinophil oxygen metabolism, tissue hypoxia, and impaired claudin-1 barrier via repression of HIF-1α/claudin-1 signaling, which was restored by transgenic expression of esophageal epithelial-targeted stabilized HIF-1α. (jci.org)
  • Furthermore, decreased expression of claudin-3 was obtained in the colonic epithelium from the c-Kit loss-of-function mutant mice. (mdpi.com)
  • In addition, metformin activated AMPK phosphorylation, inhibited NF-κB activation, down-regulated cytokine (IL-1β, IL-6, TNF-α) and ICAM-1 expression following tMCAO (P (nih.gov)
  • We aimed our study to investigate whether claudin protein expression and methylation of CLDN can influence the tumorigenesis of colorectal cancer. (diva-portal.org)
  • Significantly, in invasive human breast tumours, high levels of Snail and Slug correlated with low levels of Claudin - 1 expression. (biochemj.org)
  • Expression of ZO-1 was diffuse in all groups, with the highest intensity in the superficial epithelium in the control group. (nih.gov)
  • Using a high throughput inhibitor screen, we identified high expression of glioma-associated oncogene homolog 1 ( GLI1) , the effector molecule of the hedgehog (Hh) pathway, as a critical determinant of cell lines that have undergone an epithelial to mesenchymal transition (EMT). (biomedcentral.com)
  • Immunohistochemical analysis of this patient cohort revealed a significant association between high claudin 1 expression and BLBCs in women 55 years of age and older. (biomedcentral.com)
  • More importantly, our studies strongly suggest that claudin 1 directly participates in promoting breast cancer progression, possibly through the alteration of expression of EMT genes. (biomedcentral.com)
  • Several authors have reported conflicting results of claudin-1 expression in colorectal carcinoma. (oatext.com)
  • The goal of this study is to examine claudin-1 expression in a tissue microarray of colorectal cancer and metastases, simultaneously, in order to assess the prognostic value of this protein in colorectal cancer. (oatext.com)
  • This is the first study to demonstrate the expression of claudin-1 protein across a sample of colorectal cancer and its metastases, simultaneously. (oatext.com)
  • We report loss of claudin-1 expression in the lymph node metastases in the majority of cases. (oatext.com)
  • Claudin-1 expression is frequently altered in several cancers, including upregulation and down regulation [13]. (oatext.com)
  • miR-1303 could bind to the putative binding sites in CLDN18 mRNA 3′-UTR and visibly lower the expression of claudin-18. (springer.com)
  • Immunohistochemical analysis was performed to evaluate the ASK1 protein expression and the correlation between ASK1, claudin-6 and clinicopathological features in 85 samples of breast invasive ductal carcinomas (IDC). (biomedcentral.com)
  • Western blotting and RT-PCR was carried out to examine the expression of ASK1 and claudin-6 in MCF-7 cell clones transfected with claudin-6. (biomedcentral.com)
  • Del Zoppo, Gregory J. / Interendothelial claudin-5 expression depends on cerebral endothelial cell-matrix adhesion by Β 1-integrins . (elsevier.com)
  • Deletion of claudin-7 reduced integrin α2 expression and altered its localization. (aacrjournals.org)
  • Disruption of tight junctions, including changes in claudin-5 expression, is common in HIV-1-infected patients. (elsevier.com)
  • Andras, IE & Toborek, MJ 2011, HIV-1-induced alterations of claudin-5 expression at the blood-brain barrier level . (elsevier.com)
  • U0126 treatment also induced claudin-2 expression and decreased TER in a high resistance mouse cortical collecting duct cell line (94D). (uab.edu)
  • A significant association was seen between claudin-1 and Ecadherin expression (p=0.002), as well with β-catenin (p=0.009). (ejmjih.com)
  • However, the expression of claudin-6 in breast invasive ductal carcinomas and correlation with clinical behavior or expression of other markers is unclear. (biomedcentral.com)
  • Claudin-6 expression was inversely correlated with lymph node metastasis ( P = 0.021). (biomedcentral.com)
  • Transepithelial resistance and claudin expression in trout RTgill-W1 cell line: Effects of osmoregulatory hormones. (genscript.com)
  • Abnormal expression and mislocalization of Claudin 7 are frequently observed in epithelial-derived lung, breast, head, and neck cancers. (perkinelmer.com)
  • PAR-1 and -2 were localized intracellularly and in close proximity to lateral surfaces beneath tight junctions, and expression was increased in colonic mucosa from individuals with Crohn's disease. (jimmunol.org)
  • Chromatin immunoprecipitation (ChIP) and luciferase reporter assay showed that SCF/c-kit signaling significantly promoted activator protein-1 (AP-1) binding with CLDN-3 promoter and enhanced its transcription activity. (mdpi.com)
  • Regression analysis however, showed a significant positive association between claudin 1 and claudin 4, even though claudin 4 did not significantly correlate with patient age. (biomedcentral.com)
  • The intestinal bacterial richness (Chao 1 and OTUs) was significantly lower in OAP than in NAP rats. (nature.com)
  • HIV-1 crosses the blood-brain barrier (BBB) early in the course of systemic infection and resides in brain macrophages and microglia. (elsevier.com)
  • Objective To explore the effects of N-butylphthalide on the expressions of ZO-1 and claudin-5 in blood-brain barrier (BBB) in rats with acute carbon monoxide (CO) poisoning . (bvsalud.org)
  • Among its related pathways are Sertoli-Sertoli Cell Junction Dynamics and Blood-Brain Barrier and Immune Cell Transmigration: VCAM-1/CD106 Signaling Pathways . (genecards.org)
  • Materials and Methods: Surgical specimens from 69 patients with colorectal cancer were immunostained for claudin-1, E-cadherin, β-catenin, endoglin and CD31. (ejmjih.com)
  • Using immunofluorescent microscopy and biochemistry methods, we found that claudin-7 co-localized and co-immunoprecipitated with integrin α2 in Cldn7 +/+ mouse intestines where claudin-7 was highly expressed. (aacrjournals.org)
  • However, recent studies suggest that claudin 1 contributes to the progression of some molecular subtypes of breast cancer. (sigmaaldrich.com)
  • Collectively, our results suggest that claudin 1 has the potential to be used as a predictive marker for treatment efficacy for specific breast cancer patient subgroups. (sigmaaldrich.com)
  • Human claudin-1 and claudin-3, fused to ECFP or EYFP at the N- or C-terminus, were expressed in the TJ-free cell line HEK (human embryonic kidney)-293. (nih.gov)
  • We also demonstrate by band-shift assay that Snail and Slug bind to the E-box motifs present in the human Claudin - 1 promoter. (biochemj.org)
  • The tight junction (TJ) protein claudin-2 is overexpressed in human CRC where it enhances cell proliferation, colony formation, and chemoresistance in vitro. (aacrjournals.org)
  • The AlphaLISA ® immunoassay kit for human claudin 7 enables the detection and quantitation of claudin 7 in cell culture media and human cell lysates using a homogeneous AlphaLISA assay (no wash steps). (perkinelmer.com)
  • Human claudin-low breast cancers carried a distinct set of genomic characteristics, in particular a relatively low burden of mutations and copy number aberrations. (uio.no)
  • Reactivity has been confirmed with rat, human and mouse Claudin-5 using rat lung, mouse kidney, mouse small intestine, mouse lung homogenates, human colon tissue, and CACO-2 human cell line. (thermofisher.com)
  • Role of claudin species-specific dynamics in reconstitution and remodeling of the zonula occludens. (ebi.ac.uk)
  • Manner of interaction of heterogeneous claudin species within and between tight junction strands. (ebi.ac.uk)
  • Most cell types express more than two claudin species in various combinations to constitute TJ strands. (biologists.org)
  • Claudin molecules bear four transmembrane domains with both N- and C-termini located in the cytoplasm. (biologists.org)
  • While several of these biological processes are features of the CSC phenotype, a role for claudin-2 in the regulation of these has not been identified. (aacrjournals.org)
  • Among these, miR-222-3p was instrumental for the regulation of self-renewal by claudin-2, and enhancement of this self-renewal required activation of YAP, most likely upstream from miR-222-3p. (aacrjournals.org)
  • The mechanisms of claudin regulation and their exact roles in normal physiology and disease are being elucidated, but much work remains to be done. (hindawi.com)
  • Down-regulation of survivin and hypoxia-inducible factor-1 α by β-elemene enhances the radiosensitivity of lung adenocarcinoma xenograft. (greenmedinfo.com)
  • During HCV infection claudin-1 is highly expressed in liver and believed to be associated with HCV virus entry after HCV binding with or without co-receptor CD81. (biomedcentral.com)
  • One family member, claudin-1, also associates with tetraspanin CD81 as part of a receptor complex that is essential for hepatitis C virus (HCV) infection of the liver. (chalmers.se)
  • To understand the molecular basis of claudin-1/CD81 association we previously produced and purified milligram quantities of functional, full-length CD81, which binds a soluble form of HCV E2 glycoprotein (sE2). (chalmers.se)
  • Dynamic light scattering demonstrated that claudin-1 oligomers associate with CD81 in vitro in a defined molar ratio of 1:2 and that complex formation was enhanced by the presence of cholesteryl hemisuccinate. (chalmers.se)
  • We conclude that recombinant, correctly-folded, full-length claudin-1 can be produced in yeast membranes, that it can be extracted in different oligomeric forms that do not bind sE2 and that a dynamic preparation can form a specific complex with CD81 in vitro in the absence of any other cellular components. (chalmers.se)
  • These findings pave the way for the structural characterization of claudin-1 alone and in complex with CD81. (chalmers.se)
  • CD81 and claudin 1 coreceptor association: role in hepatitis C virus entry. (ox.ac.uk)
  • In silico directed mutagenesis identifies the CD81/claudin-1 hepatitis C virus receptor interface. (ox.ac.uk)
  • We explored the molecular interface between CD81 and claudin-1 using a combination of bioinformatic sequence-based modelling, site-directed mutagenesis and Fluorescent Resonance Energy Transfer (FRET) imaging methodologies. (ox.ac.uk)
  • Structural modelling predicts the first extracellular loop of claudin-1 to have a flexible beta conformation and identifies a motif between amino acids 62-66 that interacts with CD81 residues T149, E152 and T153. (ox.ac.uk)
  • FRET studies confirm a role for these CD81 residues in claudin-1 association and HCV infection. (ox.ac.uk)
  • We also observed an inverse relationship between upregulation of claudin 1 and TGFβ. (sigmaaldrich.com)
  • In vertebrates, canonical Hedgehog (Hh) pathway signal transduction occurs when one of the three ligands, Sonic, Indian, or Desert hedgehog, binds to the receptor Patched-1 ( PTCH1 ) or its homolog, Patched-2. (biomedcentral.com)
  • Receptor tyrosine kinase/Ras and transforming growth factor (TGF)-β signaling as well as Wnt/β-catenin-, Notch-, Hedgehog-, and NF-κB-dependent pathways can induce and maintain EMT during cancer progression ( 1 , 3 ). (aacrjournals.org)
  • In the present study, we demonstrated that chronic FS stress (CFSS) could activate corticotropin-releasing factor (CRF)/CRF receptor type 1 (CRFR1) signaling in the BLA, and blockade of CRF/CRFR1 signaling by intra-BLA injection of NBI27914 (NBI), a selective CRFR1 antagonist, could prevent the CFSS-induced depressive-like behaviors in rats, indicating that activation of CRF/CRFR1 signaling in the BLA is required for CFSS-induced depression. (bireme.br)
  • The effect of medullary NaCl loading on Na excretion was determined in intact and ovariectomized (OVX) female Sprague-Dawley rats with and without ET-1 or P2 receptor antagonism. (bireme.br)
  • Experiment in isolated renal proximal tubules of OZR revealed that both the agonists C21 and Ang-(1-7) stimulated NO which was blocked by either of the receptor antagonists. (bireme.br)
  • Claudin-3 is a major protein of tight junctions (TJs) in the intestinal epithelium and is critical for maintaining cell-cell adhesion, barrier function, and epithelium polarity. (mdpi.com)
  • Claudin-1 is an integral membrane protein component of tight junctions. (biochemj.org)
  • The claudin-1 assembly with tight junctions is regulated by post translational modifications. (biomedcentral.com)
  • and Thr 191 may provide an on/off switch to regulate assembly of claudin-1 at tight junctions. (biomedcentral.com)
  • In addition to that, tight junctions also play critical roles in maintaining cell polarity and signal transductions [1,2]. (oatext.com)
  • The integrity of the brain endothelium is regulated by intercellular tight junctions, which also play a critical role in HIV-1-entry into the brain. (elsevier.com)
  • Thus, Claudin-4 forms channels through the tight junctions that discriminate against Na+ ions and are indifferent to Cl- ions. (thermofisher.com)
  • Objectives: Intercellular adhesion mediated by claudin and cadherin/catenin complex is a prerequisite of epithelial integrity and differentiation and has been suggested to be frequently disturbed in cancers. (ejmjih.com)
  • Measuring the Claudin 7 levels is important for the detection of different types of cancers and is also an important target for therapeutic intervention. (perkinelmer.com)
  • Claudin-6 was identified through searching expressed sequence tag (EST) databases for sequences similar to claudin-1 and -2 [ PMID: 9892664 ]. (ebi.ac.uk)
  • The structure shows that C-CPE forms extensive hydrophobic and hydrophilic interactions with the two extracellular segments of claudin-19. (rcsb.org)
  • Bartholow TL, Chandran UR , Becich MJ , Parwani AV. Immunohistochemical profiles of claudin-3 in primary and metastatic prostatic adenocarcinoma. (pitt.edu)
  • 4. The skin care composition of claim 1, wherein said pomegranate seed oil is present in an amount from about 0.001 to about 20% by weight of total composition ingredients. (freepatentsonline.com)
  • Claudin-6 shares ~25-70% overall similarity with other claudin family members at the amino acid level, displaying highest similarity to claudin-9. (ebi.ac.uk)
  • Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. (ebi.ac.uk)
  • Belongs to the claudin family. (abcam.com)
  • 1 Growing evidence indicates that endothelial cell-to-cell adhesion is accompanied by intracellular signaling. (ahajournals.org)
  • TGF-β) receptors, FAK plays a central role in transducing signals to the actin cytoskeleton to elicit F-actin remodeling (e.g., formation of lamellipodia and stress fibers) during cell adhesion and migration ( 1 , 7 ). (pnas.org)
  • Western blotting and RT-PCR consistently revealed that the level of ASK1 protein and mRNA was upregulated in MCF-7 cell clones transfected with claudin-6. (biomedcentral.com)
  • Suppression of claudin-7 also upregulated matrix metalloproteinase-3 (MMP-3) at both mRNA and protein levels and downregulated several collagens, such as type V α1, type XV α1, and type XVI α1, at the mRNA level. (aacrjournals.org)
  • The data provide novel insight into the molecular TJ architecture consistent with a model with an antiparallel double-row cis-arrangement of classic claudin protomers within strands. (nih.gov)
  • Claudin-low breast cancer is a molecular subtype associated with poor prognosis and without targeted treatment options. (uio.no)
  • Our results uncover crosstalk between NFκB and GLI1 signals and suggest that targeting these pathways may be effective against the claudin-low breast cancer subtype. (biomedcentral.com)
  • Therefore, the purpose of the current study is to discover the relationship between ASK1 and claudin-6 in breast cancer and to explore the pathways involves the activation of ASK1. (biomedcentral.com)
  • In this study, the localization of claudin-1 in maturation ameloblasts was examined using immunofluorescence microscopy. (frontiersin.org)
  • Your search returned 4 Claudin 7 ELISA ELISA Kit across 1 supplier. (biocompare.com)
  • Knocking down Sec24C reduced the cell surface level of claudin-1. (asm.org)
  • Blocking COPII transport by XN decreased the cell surface level of claudin-1. (asm.org)
  • Moreover, an inverse correlation in the levels of Claudin - 1 and Slug transcripts were observed in breast cancer cell lines. (biochemj.org)
  • E-box elements in the Claudin - 1 promoter were found to play a critical negative regulatory role in breast cancer cell lines that expressed low levels of Claudin - 1 transcript. (biochemj.org)
  • We observed non-canonical activation of GLI1 in claudin-low and EMT cell lines, and identified crosstalk with the NFκB pathway. (biomedcentral.com)
  • We further provide direct evidence for the binding of the NFκB subunit p65 to the GLI1 promoter in both EMT and claudin-low cell lines. (biomedcentral.com)
  • in particular, SERPINE 1 (PAI1) and SSP1 (osteopontin), known to inhibit EMT and cancer cell migration. (biomedcentral.com)
  • Claudin-2 enhanced self-renewal of ALDHHigh CSC and increased their proportion in CRC cell populations, limiting their differentiation and promoting the phenotypic transition of non-CSC towards the ALDHHigh phenotype. (aacrjournals.org)
  • Thus, our current study highlights a novel non-TJ function of claudin-7 in maintaining epithelial cell-matrix interactions by interacting with integrins. (aacrjournals.org)
  • This is attributable to the tightly packed actin filament bundles sandwiched between cisternae of endoplasmic reticulum and the apposing Sertoli cell plasma membrane at the basal ES, the hallmark ultrastructure of the BTB ( 1 ). (pnas.org)