Clathrin Light Chains: The light chain subunits of clathrin.Clathrin: The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.Clathrin Heavy Chains: The heavy chain subunits of clathrin.Clathrin-Coated Vesicles: Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.Coated Pits, Cell-Membrane: Specialized regions of the cell membrane composed of pits coated with a bristle covering made of the protein CLATHRIN. These pits are the entry route for macromolecules bound by cell surface receptors. The pits are then internalized into the cytoplasm to form the COATED VESICLES.Endocytosis: Cellular uptake of extracellular materials within membrane-limited vacuoles or microvesicles. ENDOSOMES play a central role in endocytosis.Brain Chemistry: Changes in the amounts of various chemicals (neurotransmitters, receptors, enzymes, and other metabolites) specific to the area of the central nervous system contained within the head. These are monitored over time, during sensory stimulation, or under different disease states.Cattle: Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.Immunoglobulin Light Chains: Polypeptide chains, consisting of 211 to 217 amino acid residues and having a molecular weight of approximately 22 kDa. There are two major types of light chains, kappa and lambda. Two Ig light chains and two Ig heavy chains (IMMUNOGLOBULIN HEAVY CHAINS) make one immunoglobulin molecule.Myosin Light Chains: The smaller subunits of MYOSINS that bind near the head groups of MYOSIN HEAVY CHAINS. The myosin light chains have a molecular weight of about 20 KDa and there are usually one essential and one regulatory pair of light chains associated with each heavy chain. Many myosin light chains that bind calcium are considered "calmodulin-like" proteins.Light: That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.Amino Acid Sequence: The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.Protein Binding: The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.Microscopy, Fluorescence: Microscopy of specimens stained with fluorescent dye (usually fluorescein isothiocyanate) or of naturally fluorescent materials, which emit light when exposed to ultraviolet or blue light. Immunofluorescence microscopy utilizes antibodies that are labeled with fluorescent dye.Monomeric Clathrin Assembly Proteins: A subclass of clathrin assembly proteins that occur as monomers.src-Family Kinases: A PROTEIN-TYROSINE KINASE family that was originally identified by homology to the Rous sarcoma virus ONCOGENE PROTEIN PP60(V-SRC). They interact with a variety of cell-surface receptors and participate in intracellular signal transduction pathways. Oncogenic forms of src-family kinases can occur through altered regulation or expression of the endogenous protein and by virally encoded src (v-src) genes.Phosphorylation: The introduction of a phosphoryl group into a compound through the formation of an ester bond between the compound and a phosphorus moiety.Receptors, Antigen, T-Cell: Molecules on the surface of T-lymphocytes that recognize and combine with antigens. The receptors are non-covalently associated with a complex of several polypeptides collectively called CD3 antigens (ANTIGENS, CD3). Recognition of foreign antigen and the major histocompatibility complex is accomplished by a single heterodimeric antigen-receptor structure, composed of either alpha-beta (RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA) or gamma-delta (RECEPTORS, ANTIGEN, T-CELL, GAMMA-DELTA) chains.Jurkat Cells: A CELL LINE derived from human T-CELL LEUKEMIA and used to determine the mechanism of differential susceptibility to anti-cancer drugs and radiation.Tyrosine: A non-essential amino acid. In animals it is synthesized from PHENYLALANINE. It is also the precursor of EPINEPHRINE; THYROID HORMONES; and melanin.Polypyrimidine Tract-Binding Protein: A RNA-binding protein that binds to polypyriminidine rich regions in the INTRONS of messenger RNAs. Polypyrimidine tract-binding protein may be involved in regulating the ALTERNATIVE SPLICING of mRNAs since its presence on an intronic RNA region that is upstream of an EXON inhibits the splicing of the exon into the final mRNA product.RNA-Binding Proteins: Proteins that bind to RNA molecules. Included here are RIBONUCLEOPROTEINS and other proteins whose function is to bind specifically to RNA.Ribonucleoproteins: Complexes of RNA-binding proteins with ribonucleic acids (RNA).Heterogeneous-Nuclear Ribonucleoproteins: A family of ribonucleoproteins that were originally found as proteins bound to nascent RNA transcripts in the form of ribonucleoprotein particles. Although considered ribonucleoproteins they are primarily classified by their protein component. They are involved in a variety of processes such as packaging of RNA and RNA TRANSPORT within the nucleus. A subset of heterogeneous-nuclear ribonucleoproteins are involved in additional functions such as nucleocytoplasmic transport (ACTIVE TRANSPORT, CELL NUCLEUS) of RNA and mRNA stability in the CYTOPLASM.Ribosomes: Multicomponent ribonucleoprotein structures found in the CYTOPLASM of all cells, and in MITOCHONDRIA, and PLASTIDS. They function in PROTEIN BIOSYNTHESIS via GENETIC TRANSLATION.Protein Biosynthesis: The biosynthesis of PEPTIDES and PROTEINS on RIBOSOMES, directed by MESSENGER RNA, via TRANSFER RNA that is charged with standard proteinogenic AMINO ACIDS.5' Untranslated Regions: The sequence at the 5' end of the messenger RNA that does not code for product. This sequence contains the ribosome binding site and other transcription and translation regulating sequences.Interleukin Receptor Common gamma Subunit: An interleukin receptor subunit that was originally discovered as a component of the INTERLEUKIN 2 RECEPTOR. It was subsequently found to be a component of several other receptors including the INTERLEUKIN 4 RECEPTOR, the INTERLEUKIN 7 RECEPTOR, the INTERLEUKIN-9 RECEPTOR, the INTERLEUKIN-15 RECEPTOR, and the INTERLEUKIN-21 RECEPTOR. Mutations in the gene for the interleukin receptor common gamma chain have been associated with X-LINKED COMBINED IMMUNODEFICIENCY DISEASES.Search Engine: Software used to locate data or information stored in machine-readable form locally or at a distance such as an INTERNET site.Mice, Inbred NOD: A strain of non-obese diabetic mice developed in Japan that has been widely studied as a model for T-cell-dependent autoimmune insulin-dependent diabetes mellitus in which insulitis is a major histopathologic feature, and in which genetic susceptibility is strongly MHC-linked.Databases, Genetic: Databases devoted to knowledge about specific genes and gene products.Mice, SCID: Mice homozygous for the mutant autosomal recessive gene "scid" which is located on the centromeric end of chromosome 16. These mice lack mature, functional lymphocytes and are thus highly susceptible to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency (SCID) syndrome in human infants. SCID mice are useful as animal models since they are receptive to implantation of a human immune system producing SCID-human (SCID-hu) hematochimeric mice.Genome, Human: The complete genetic complement contained in the DNA of a set of CHROMOSOMES in a HUMAN. The length of the human genome is about 3 billion base pairs.Antibodies: Immunoglobulin molecules having a specific amino acid sequence by virtue of which they interact only with the ANTIGEN (or a very similar shape) that induced their synthesis in cells of the lymphoid series (especially PLASMA CELLS).Apomorphine: A derivative of morphine that is a dopamine D2 agonist. It is a powerful emetic and has been used for that effect in acute poisoning. It has also been used in the diagnosis and treatment of parkinsonism, but its adverse effects limit its use.Emetics: Agents that cause vomiting. They may act directly on the gastrointestinal tract, bringing about emesis through local irritant effects, or indirectly, through their effects on the chemoreceptor trigger zone in the postremal area near the medulla.Receptors, Dopamine D2: A subfamily of G-PROTEIN-COUPLED RECEPTORS that bind the neurotransmitter DOPAMINE and modulate its effects. D2-class receptor genes contain INTRONS, and the receptors inhibit ADENYLYL CYCLASES.Morphine: The principal alkaloid in opium and the prototype opiate analgesic and narcotic. Morphine has widespread effects in the central nervous system and on smooth muscle.Databases, Pharmaceutical: Databases devoted to knowledge about PHARMACEUTICAL PRODUCTS.Dopamine: One of the catecholamine NEUROTRANSMITTERS in the brain. It is derived from TYROSINE and is the precursor to NOREPINEPHRINE and EPINEPHRINE. Dopamine is a major transmitter in the extrapyramidal system of the brain, and important in regulating movement. A family of receptors (RECEPTORS, DOPAMINE) mediate its action.Pharmacological Processes: The metabolism of drugs and their mechanisms of action.Atlases as Topic: Collections of illustrative plates, charts, etc., usually with explanatory captions.Hip Joint: The joint that is formed by the articulation of the head of FEMUR and the ACETABULUM of the PELVIS.Hip: The projecting part on each side of the body, formed by the side of the pelvis and the top portion of the femur.Proteomics: The systematic study of the complete complement of proteins (PROTEOME) of organisms.Arthroplasty, Replacement, Hip: Replacement of the hip joint.Osteoarthritis, Hip: Noninflammatory degenerative disease of the hip joint which usually appears in late middle or old age. It is characterized by growth or maturational disturbances in the femoral neck and head, as well as acetabular dysplasia. A dominant symptom is pain on weight-bearing or motion.Hip Fractures: Fractures of the FEMUR HEAD; the FEMUR NECK; (FEMORAL NECK FRACTURES); the trochanters; or the inter- or subtrochanteric region. Excludes fractures of the acetabulum and fractures of the femoral shaft below the subtrochanteric region (FEMORAL FRACTURES).Patents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Brain Injuries: Acute and chronic (see also BRAIN INJURIES, CHRONIC) injuries to the brain, including the cerebral hemispheres, CEREBELLUM, and BRAIN STEM. Clinical manifestations depend on the nature of injury. Diffuse trauma to the brain is frequently associated with DIFFUSE AXONAL INJURY or COMA, POST-TRAUMATIC. Localized injuries may be associated with NEUROBEHAVIORAL MANIFESTATIONS; HEMIPARESIS, or other focal neurologic deficits.Dietary Supplements: Products in capsule, tablet or liquid form that provide dietary ingredients, and that are intended to be taken by mouth to increase the intake of nutrients. Dietary supplements can include macronutrients, such as proteins, carbohydrates, and fats; and/or MICRONUTRIENTS, such as VITAMINS; MINERALS; and PHYTOCHEMICALS.Coumaric Acids: Hydroxycinnamic acid and its derivatives. Act as activators of the indoleacetic acid oxidizing system, thereby producing a decrease in the endogenous level of bound indoleacetic acid in plants.Work Capacity Evaluation: Assessment of physiological capacities in relation to job requirements. It is usually done by measuring certain physiological (e.g., circulatory and respiratory) variables during a gradually increasing workload until specific limitations occur with respect to those variables.Physical Exertion: Expenditure of energy during PHYSICAL ACTIVITY. Intensity of exertion may be measured by rate of OXYGEN CONSUMPTION; HEAT produced, or HEART RATE. Perceived exertion, a psychological measure of exertion, is included.Physical Fitness: The ability to carry out daily tasks and perform physical activities in a highly functional state, often as a result of physical conditioning.
Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo. (1/30)Clathrin heavy and light chains form triskelia, which assemble into polyhedral coats of membrane vesicles that mediate transport for endocytosis and organelle biogenesis. Light chain subunits regulate clathrin assembly in vitro by suppressing spontaneous self-assembly of the heavy chains. The residues that play this regulatory role are at the N terminus of a conserved 22-amino acid sequence that is shared by all vertebrate light chains. Here we show that these regulatory residues and others in the conserved sequence mediate light chain interaction with Hip1 and Hip1R. These related proteins were previously found to be enriched in clathrin-coated vesicles and to promote clathrin assembly in vitro. We demonstrate Hip1R binding preference for light chains associated with clathrin heavy chain and show that Hip1R stimulation of clathrin assembly in vitro is blocked by mutations in the conserved sequence of light chains that abolish interaction with Hip1 and Hip1R. In vivo overexpression of a fragment of clathrin light chain comprising the Hip1R-binding region affected cellular actin distribution. Together these results suggest that the roles of Hip1 and Hip1R in affecting clathrin assembly and actin distribution are mediated by their interaction with the conserved sequence of clathrin light chains. (+info)
Huntingtin interacting protein 1 (HIP1) regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain. (2/30)Huntingtin interacting protein 1 (HIP1) is a component of clathrin coats. We previously demonstrated that HIP1 promotes clathrin assembly through its central helical domain, which binds directly to clathrin light chains (CLCs). To better understand the relationship between CLC binding and clathrin assembly we sought to dissect this interaction. Using C-terminal deletion constructs of the HIP1 helical domain, we identified a region between residues 450 and 456 that is required for CLC binding. Within this region, point mutations showed the importance of residues Leu-451, Leu-452, and Arg-453. Mutants that fail to bind CLC are unable to promote clathrin assembly in vitro but still mediate HIP1 homodimerization and heterodimerization with the family member HIP12/HIP1R. Moreover, HIP1 binding to CLC is necessary for HIP1 targeting to clathrin-coated pits and clathrin-coated vesicles. Interestingly, HIP1 binds to a highly conserved region of CLC previously demonstrated to regulate clathrin assembly. These results suggest a role for HIP1/CLC interactions in the regulation of clathrin assembly. (+info)
Clathrin heavy and light chain isoforms originated by independent mechanisms of gene duplication during chordate evolution. (3/30)In humans, there are two isoforms each of clathrin heavy chain (CHC17 and CHC22) and light chain (LCa and LCb) subunits, all encoded by separate genes. CHC17 forms the ubiquitous clathrin-coated vesicles that mediate membrane traffic. CHC22 is implicated in specialized membrane organization in skeletal muscle. CHC17 is bound and regulated by LCa and LCb, whereas CHC22 does not functionally interact with either light chain. The imbalanced interactions between clathrin subunit isoforms suggest a distinct evolutionary history for each isoform pair. Phylogenetic and sequence analysis placed both heavy and light chain gene duplications during chordate evolution, 510-600 million years ago. Genes encoding CHC22 orthologues were found in several vertebrate species, with only a pseudogene present in mice. Multiple paralogons surrounding the CHC genes (CLTC and CLTD) were identified, evidence that genomic or large-scale gene duplication produced the two CHC isoforms. In contrast, clathrin light chain genes (CLTA and CLTB) apparently arose by localized duplication, within 1-11 million years of CHC gene duplication. Analysis of sequence divergence patterns suggested that structural features of the CHCs were maintained after gene duplication, but new interactions with regulatory proteins evolved for the CHC22 isoform. Thus, independent mechanisms of gene duplication expanded clathrin functions, concomitant with development of neuromuscular sophistication in chordates. (+info)
Non-stoichiometric relationship between clathrin heavy and light chains revealed by quantitative comparative proteomics of clathrin-coated vesicles from brain and liver. (4/30)We used tandem mass spectrometry with peptide counts to identify and to determine the relative levels of expression of abundant protein components of highly enriched clathrin-coated vesicles (CCVs) from rat liver. The stoichiometry of stable protein complexes including clathrin heavy chain and clathrin light chain dimers and adaptor protein (AP) heterotetramers was assessed. We detected a deficit of clathrin light chain compared with clathrin heavy chain in non-brain tissues, suggesting a level of regulation of clathrin cage formation specific to brain. The high ratio of AP-1 to AP-2 in liver CCVs is reversed compared with brain where there is more AP-2 than AP-1. Despite this, general endocytic cargo proteins were readily detected in liver but not in brain CCVs, consistent with the previous demonstration that a major function for brain CCVs is recycling synaptic vesicles. Finally we identified 21 CCV-associated proteins in liver not yet characterized in mammals. Our results further validate the peptide accounting approach, reveal new information on the properties of CCVs, and allow for the use of quantitative proteomics to compare abundant components of organelles under different experimental and pathological conditions. (+info)
Membrane-associated STAT3 and PY-STAT3 in the cytoplasm. (5/30)Signal transduction from the plasma membrane to the nucleus by STAT proteins is widely represented as exclusively a soluble cytosolic process. Using cell-fractionation methods, we observed that approximately 5% of cytoplasmic STAT3 was constitutively associated with the purified early endosome (EE) fraction in human Hep3B liver cells. By 15-30 min after interleukin-6 (IL-6) treatment, up to two-thirds of cytoplasmic Tyr-phosphorylated STAT3 can be associated with the purified early endosome fraction (Rab-5-, EEA1-, transferrin receptor-, and clathrin-positive fraction). Electron microscopy, immunofluorescence, and detergent dissection approaches confirmed the association of STAT3 and PY-STAT3 with early endosomes. STAT3 was constitutively associated with clathrin heavy chain in membrane and in the 1- to 2-MDa cytosolic complexes. The membrane association was dynamic in that, within 15 min of treatment with the vicinal-thiol cross-linker phenylarsine oxide, there was a dramatic increase in bulk STAT3 association with sedimentable membranes. The functional contribution of PY-STAT3 association with the endocytic pathway was evaluated in transient transfection assays using IL-6-inducible STAT3-reporter-luciferase constructs and selective regulators of this pathway. STAT3-transcriptional activation was inhibited by expression constructs for dominant negative dynamin K44A, epsin 2a, amphiphysin A1, and clathrin light chain but enhanced by that for the active dynamin species MxA. Taken together, these studies emphasize the contribution of the endocytic pathway to productive IL-6/STAT3 signaling. (+info)
Clathrin light chain b is capable of affecting potently a major protein phosphatase from microtubules (MT-PP1). (6/30)Clathrin light chain (CL) b purified from bovine brain postmicrotubule supernatant and identified by mass spectrometry potently inhibited a catalytic activity of a major protein phosphatase (PP) that was copurified with microtubules and recognized by antiPP1 antibodies. CLb similarly affected the catalytic subunit and holoenzyme of the PP, little inhibiting the activity of PP2A. Although the CLb from clathrin-coated vesicles was several hundredfold weaker than our purified CLb, the CLb in the postmicrotubule supernatant, independent of whether it was sedimentable or soluble, was as active as the purified CLb. Thus CLb may be a potent regulator of the PP. (+info)
Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. (7/30)In the central nervous system, clathrin-mediated endocytosis is crucial for efficient synaptic transmission. Clathrin-coated vesicle assembly and disassembly is regulated by some 30 adaptor and accessory proteins, most of which interact with clathrin heavy chain. Using the calcyon cytosolic domain as bait, we isolated clathrin light chain in a yeast two-hybrid screen. The interaction domain was mapped to the heavy chain binding domain and C-terminal regions of light chain. Further, the addition of the calcyon C terminus stimulated clathrin self-assembly in a dose-dependent fashion. Calcyon, which is a single transmembrane protein predominantly expressed in brain, localized to vesicular compartments within pre- and postsynaptic structures. There was a high degree of overlap in the distribution of LC and calcyon in neuronal dendrites, spines, and cell bodies. Co-immunoprecipitation studies further suggested an association of calcyon with the clathrin-mediated endocytic machinery. Compared with controls, HEK293 cells overexpressing calcyon exhibited significantly enhanced transferrin uptake but equivalent levels of recycling. Conversely, transferrin uptake was largely abolished in neocortical neurons obtained from mice homozygous for a calcyon null allele, whereas recycling proceeded at wild type levels. Collectively, these data indicate a role for calcyon in clathrin-mediated endocytosis in brain. (+info)
Novel function of clathrin light chain in promoting endocytic vesicle formation. (8/30)Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts with members of the Hip1/R family of endocytic proteins, including the budding yeast homologue, Sla2p. Here, using in vivo analysis in yeast, we provide novel insight into the role of this interaction. We find that overexpression of LC partially restores endocytosis to cells lacking clathrin HC. This suppression is dependent on the Sla2p binding region of LC. Using live cell imaging techniques to visualize endocytic vesicle formation, we find that the N-terminal Sla2p binding region of LC promotes the progression of arrested Sla2p patches that form in the absence of HC. We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation. (+info)
Identification of a novel interaction with clathrin light chain". J. Biol. Chem. 277 (22): 19897-904. doi:10.1074/jbc. ... 2004). "Proteomic identification of brain proteins that interact with dynein light chain LC8". Proteomics. 4 (2): 339-46. doi: ... 2002). "HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. ...
Xiao J, Dai R, Negyessy L, Bergson C (2006). "Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated ...
Clathrin, light chain B is a protein in humans that is encoded by the CLTB gene. Clathrin is a large, soluble protein composed ... This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative ... of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits ... Clathrin, light chain B". Retrieved 2013-06-05. ...
Huntingtin-interacting protein 1
HIP1's non-pathological activity includes clathrin assembly via interaction with clathrin light chains. HIP1 is the human ... regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain". The Journal of ... Actin binding by Hip-1 is altered depending on whether clathrin is also bound to Hip-1. HIP1 has also been found to be ...
"Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB)". Genomics. 24 (3): 440-4 ...
... binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis". Cell. 62 (5): 875-87. doi: ... Another function of Hsc70 is as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane ... It works with auxilin to remove clathrin coated vesicles. In neurons, synaptojanin is also an important protein involved in ...
"Analysis of clathrin light chain-heavy chain interactions using truncated mutants of rat liver light chain LCB3". J. Biol. Chem ... The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. CLTC has been shown to ... Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the ... "Entrez Gene: CLTC clathrin, heavy chain (Hc)". Tebar, F; Bohlander S K; Sorkin A (Aug 1999). "Clathrin assembly lymphoid ...
There are two forms of clathrin light chains, designated a and b. The main clathrin heavy chain, located on chromosome 17 in ... The three heavy chains provide the structural backbone of the clathrin lattice, and the three light chains are thought to ... The clathrin triskelion is composed of three clathrin heavy chains interacting at their C-termini, each ~190 kDa heavy chain ... It forms a triskelion shape composed of three clathrin heavy chains and three light chains. When the triskelia interact they ...
Diffuse large B-cell lymphoma
HLA-B encodes a heavy chain of HLA class I, that heterodimerizes with a light chain. The HLA class I has a central role in the ... It is a coactivator for steroid hormones, and the synthesized protein is involved in clathrin-mediated endocytosis. But the ...
List of MeSH codes (D12.776.543)
... clathrin heavy chains MeSH D12.776.543.990.200.750 -- clathrin light chains MeSH D12.776.543.990.300.150 -- ADP-ribosylation ... cd98 heavy chain MeSH D12.776.543.585.200.374.750.500.500 -- antigens, cd98 light chains MeSH D12.776.543.585.200.500 -- amino ... cd98 heavy chain MeSH D12.776.543.585.200.500.500.500.500 -- antigens, cd98 light chains MeSH D12.776.543.585.200.500.500.500. ... integrin beta chains MeSH D12.776.543.750.705.408.200.249 -- antigens, cd18 MeSH D12.776.543.750.705.408.200.500 -- antigens, ...
Mann SS, Hammarback JA (1994). "Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B ... "Interactions between adaptor protein-1 of the clathrin coat and microtubules via type 1a microtubule-associated proteins". J. ... polypeptide that presumably undergoes proteolytic processing to generate the final MAP1A heavy chain and LC2 light chain. ... light chains determine distinct functional properties". J. Neurosci. 22 (6): 2106-14. PMID 11896150. Ikeda A, Zheng QY, Zuberi ...
... kD protein called clathrin light chain (CLC), forming three-legged trimers called triskelions. Vesicles selectively concentrate ... The main scaffold component of clathrin coat is the 190-kD protein called clathrin heavy chain (CHC), which is associated with ... Clathrin-coated vesicles (CCVs) are found in virtually all cells and form domains of the plasma membrane termed clathrin-coated ... Coat complexes that have been well characterized so far include coat protein-I (COP-I), COP-II, and clathrin. Clathrin coats ...
G protein-coupled receptor
... clathrin adaptor molecules); thus, the arrestin here acts as a scaffold assembling the components needed for clathrin-mediated ... This mechanism is used, for example, with rhodopsin in retina cells to compensate for exposure to bright light. In many cases, ... Cyclic AMP-dependent protein kinases (protein kinase A) are activated by the signal chain coming from the G protein (that was ... which in turn recruits another protein called clathrin. If enough receptors in the local area recruit clathrin in this manner, ...
G protein-coupled receptor
... clathrin adaptor molecules); thus, the arrestin here acts as a scaffold assembling the components needed for clathrin-mediated ... Cyclic AMP-dependent protein kinases (protein kinase A) are activated by the signal chain coming from the G protein (that was ... This mechanism is used, for example, with rhodopsin in retina cells to compensate for exposure to bright light. In many cases, ... which in turn recruits another protein called clathrin. If enough receptors in the local area recruit clathrin in this manner, ...
Clathrin is involved in cellular endocytosis and is sequestrated by specific signaling molecules. Clathrin can attach to ... On the other hand, lipids with smaller acyl chain area to polar head group area ratio tend to curve positively, in other words ... Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between Golgi apparatus ... Clathrin binds to its receptors that are present in the membrane. C illustrates a slightly different mechanism. In this case, ...
... and translocation of the light chain into the cytosol. The light chain has zinc-dependent endopeptidase or more specifically ... clathrin-coated, single-membrane vesicles containing Atg16L, the v-SNARE VAMP7, and its partner t-SNAREs: Syntaxin-7, Syntaxin- ... Finally, after the light chain of BoNT is brought into the cytosol of the targeted neuron, it is released from the heavy chain ... The light chain is released from the heavy chain by the reduction of the disulfide bond holding the two together. The reduction ...
Lo KW, Kogoy JM, Pfister KK (2007). "The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint protein, ... Cayrol, C.; Cougoule, C.; Wright, M. (2002). "The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase ...
Forgac M (January 1999). "The vacuolar H+ -ATPase of clathrin-coated vesicles is reversibly inhibited by S-nitrosoglutathione ... Reverse transcription polymerase chain reaction studies have shown expression of the a4 subunit in the intercalated cell of the ... A relatively new technique called ancestral gene resurrection has shed new light on the evolutionary history of the V-ATPase. ...
... which activates myosin II directly by phosphorylation of the myosin light chain and also inhibits myosin phosphatase by ... large amounts of membrane material are removed via clathrin-mediated endocytosis  Eventually, the edges of the cell plate ... a striking process that is clearly visible through a light microscope. The cytokinetic furrow ingresses until a so-called ...
Fausser JL, Ungewickell E, Ruch JV, Lesot H (1994). "Interaction of vinculin with the clathrin heavy chain". J. Biochem. 114 (4 ... The recent discovery of the 3D structure sheds light on how this protein tailors its shape to perform a variety of functions. ...
MLK2 is myosin light chain kinase 2 The b2 adaptin of the AP2 complex Calcium-dependent activator protein for secretion (CADPS ... clathrin and tubulin". Biochem. J. 363 (Pt 3): 599-608. doi:10.1042/0264-6021:3630599. PMC 1222513 . PMID 11964161. O'Callaghan ...
Tubulin Acetylation Protein Isoform Post-translational modification Microtubules Clathrin Gene Ontology (GO) codes Molecular ... Isoform 4 Isoform 4 is different to the canonical sequence as the sequence of amino acids 323-333 from the canonical chain ( ... it might play an important paper in the intracilial transport of signal proteins during light-sensing signaling in ... or the clathrin coated-pit in the membrane. This is closely related to one of its main functions which is the catalysis of ...
කොලෙස්ටරෝල් - විකිපීඩියා, නිදහස් විශ්වකෝෂය
... while the bulky steroid and the hydrocarbon chain is embedded in the membrane, alongside the nonpolar fatty acid chains of the ... Low-density lipoproteins are taken into the cell by LDL receptor-mediated endocytosis in clathrin-coated pits, and then ... Photo taken under polarized light.. කොලෙස්ටරෝල්(Cholesterol) යනු ලිපිඩමය ඇලෙනසුලු ඇල්කොහොල් විශේෂයකි.මෙය සතුන් ගේ පටකය හා රුධිර ... Cholesterol is essential for the structure and function of invaginated caveolae and clathrin-coated pits, including caveola- ...
The N-terminus (one end) of a polypeptide chain (i.e., a protein) contains a few amino acids that work as an address tag, which ... The ER was observed with light microscope by Garnier in 1897, who coined the term "ergastoplasm". With electron ... either secreted constitutively with no tag or secreted in a regulatory manner involving clathrin and paired basic amino acids ... to the side-chain nitrogen of Asn. ...
Zhang C, Zhou S, Groppelli E, Pellegrino P, Williams I, Borrow P, Chain BM, Jolly C (2015). "Hybrid Spreading Mechanisms and T ... More recently, however, productive infection by pH-independent, clathrin-mediated endocytosis of HIV-1 has also been reported ... But urban migration rose, increasing sexual contacts and leading to red-light districts.. ... a polymerase chain reaction (PCR), western blot or, less commonly, an immunofluorescence assay (IFA)). Only specimens that are ...
Short linear motif
A Dynein Light Chain binding motif in Rabies virus is vital for host infection). The extent of human SLiM mimicry is surprising ... November 2004). "Evolving nature of the AP2 α-appendage hub during clathrin-coated vesicle endocytosis". BMC Genomics. 23 (22 ...
Laboratory of Molecular Biology
Barbara Pearse discovered the major components of clathrin-coated vesicles, structures formed during endocytosis, and a low ... J. A. Steitz, (1969) "Polypetide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage ... the parent MRC ensured that the quinquennial assessment had a light touch: only a brief explanation of past achievements and an ...
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Backues, S.K. and Bednarek, S.Y. (2010). Arabidopsis Dynamin-Related Protein 1A polymers bind, but do not tubulate, liposomes. Biochem. Biophys. Res. Commun. 393, 734-739 [PDF]. Konopka, C. A. and Bednarek, S. Y. (2008). Comparison of the Dynamics and Functional Redundancy of the Arabidopsis Dynamin-Related Isoforms DRP1A and DRP1C during Plant Development. Plant Physiol. 147, 1590-1602. [PDF]. Konopka, C. A., Backues, S. K. and Bednarek, S. Y. (2008). Dynamics of Arabidopsis Dynamin-Related Protein 1C and a Clathrin Light Chain at the Plasma Membrane. Plant Cell 20, 1363-1380. [PDF]. Park, S., Rancour, D.M., and Bednarek, S.Y. (2008). In Planta analysis of the cell cycle-dependent localization of AtCDC48A and its critical roles in cell division, expansion and differentiation. Plant Phys. 148, 246-258 [PDF]. Park, S., Rancour, D.M., and Bednarek, S.Y. (2007). Protein domain-domain interactions and requirements for the negative regulation of Arabidopsis CDC48/P97 by the Plant Ubiquitin Regulatory ...
Endocytosis is an essential phenomenon in eukaryotic cells. In animal cells, dynamin and clathrin play central roles in vesicle formation in the process of endocytosis, but the roles of similar proteins in plants are less well understood. Here, we observed the localization pattern and behavior of GFP-labeled ,i,Arabidopsis,/i, dynamin-related proteins (DRP1A and DRP2B), and clathrin light chain (AtCLC) around the plasma membrane in tobacco suspension cells by using variable incidence angle fluorescence microscopy (VIAFM). GFP fusions of DRP1A, DRP2B and AtCLC were observed as dot-like puncta 200-500 nm in diameter. The puncta moved to and away from the cell surface or also assembled and disassembled. The localization pattern and behavior of the puncta were similar to those of animal dynamin and clathrin signals reported previously. These results raise the possibility that DRP1A, DRP2B and AtCLC are involved in membrane trafficking around the plasma membrane, including endocytosis.. ...
Immunogen = synthetic peptide: E E D P A A A F L A Q Q E S E I A G I E N D, corresp. to amino acids 23-44 of Cow Clathrin light chain. ...
Probable palmitoyltransferase ZDHHC13 (EC 2.3.1.-) (Zinc finger DHHC domain-containing protein 13) (DHHC-13) (Huntingtin-interacting protein 14-related protein) (HIP14-related protein) (Huntingtin- interacting protein HIP3RP) (Putative NF-kappa-B-activati [Source:Uniprot/SWISSPROT;Acc:Q8IUH4 ...
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Clathrin is spindle-associated but not essential for mitosis. - Nuffield Department of Surgical Sciences
BACKGROUND: Clathrin is a multimeric protein involved in vesicle coat assembly. Recently clathrin distribution was reported to change during the cell cycle and was found to associate with the mitotic spindle. Here we test whether the recruitment of clathrin to the spindle is indicative of a critical functional contribution to mitosis. METHODOLOGY/PRINCIPAL FINDINGS: Previously a chicken pre-B lymphoma cell line (DKO-R) was developed in which the endogenous clathrin heavy chain alleles were replaced with the human clathrin heavy chain under the control of a tetracycline-regulatable promoter. Receptor-mediated and fluid-phase endocytosis were significantly inhibited in this line following clathrin knockout, and we used this to explore the significance of clathrin heavy chain expression for cell cycle progression. We confirmed using confocal microscopy that clathrin colocalised with tubulin at mitotic spindles. Using a propidium iodide flow cytometric assay we found no statistical difference in the cell
Chloride channels (ClCs) have gained worldwide interest because of their molecular diversity, widespread distribution in mammalian tissues and organs, and their link to various human diseases. Nine different ClCs have been molecularly identified and functionally characterized in mammals. ClC-2 is one of nine mammalian members of the ClC family. It possesses unique biophysical characteristics, pharmacological properties, and molecular features that distinguish it from other ClC family members. ClC-2 has wide organ/tissue distribution and is ubiquitously expressed. Published studies consistently point to a high degree of conservation of ClC-2 function and regulation across various species from nematodes to humans over vast evolutionary time spans. ClC-2 has been intensively and extensively studied over the past two decades, leading to the accumulation of a plethora of information to advance our understanding of its pathophysiological functions; however, many controversies still exist. It is necessary to
Expression of ABCB1 (ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1) in rectum tissue. Antibody staining with HPA002199 and CAB001716 in immunohistochemistry.
Summary of ABCB1 (ABC20, CD243, CLCS, GP170, MDR1, P-gp, PGY1) expression in human tissue. Membranous expression in a few tissues, most abundant in adrenal gland. Distinct expression in endothelial cells of CNS.
In this report we have addressed the biological consequence of endofin-mediated TOM1 recruitment onto endosomes. Our results collectively suggest that TOM1 serves as an adaptor for endofin to recruit clathrin heavy chain onto the endosomes. This conclusion is supported by several lines of evidence as described in our study.. First, via large-scale pull-down experiments using immobilized GST-TOM1(300-492), we have recovered clathrin heavy chain as the major and specific partner for TOM1. This conclusion was corroborated by analytical pull-down experiments showing that clathrin heavy chain was very efficiently retained by immobilized GST-TOM1(300-492), so much so that it was depleted from the cytosol. The specific interaction between TOM1 and clathrin heavy chain was further defined by our identification of three sites in the carboxyl-terminal region of TOM1, which seem to act together for efficient interaction with clathrin. Moreover, the specific blockage of interaction between TOM1 and clathrin ...
Intracellular vesicle trafficking is an important mechanism for delivering proteins to their proper location where they can function within cells. Various genes involved in vesicle trafficking have been associated with Parkinson s disease (PD) including the recently identified huntingtin-interacting protein-1 related (HIP1R) gene. We have identified a rare mutation on HIP1R in a PD patient. Our study aims to (i) identify additional mutations on HIP1R in PD patients; (ii) study genetic interactions between HIP1R, LRRK2 and SNCA; and (iii) to characterize the effects of HIP1R mutations on function.. Hypothesis ...
The structure of Clathrin is known as a triskeleion structure with there being three bent legs extending from a central point known as the central trimerization domain. Clathrin is made up of six chains of protein that are braided together in such a way to form to form its distinct shape. Three of these chains are known as heavy chains and form the backbone of Clathrin, they consist of two sub domains a N-terminal zone and a proximal leg domain. A seven bladed β-propeller structure is what the N-terminal domain consists off. While the proximal leg consists of a super Helix (Conner and Schmid, 2002). The other three chains are known as light chains and regulate formation and disassembly of the Clathrin and can be found connected to the proximal portion of the heavy chains. Multiple Clathrin molecules have the ability to form a variety of complex shapes when they interact, they can form 5 or 6 sided rings with the 5 sided kind having a greature curvature and when enough get together they can form ...
Plasmid mEos4a-Clathrin-15 from Dr. Michael Davidsons lab contains the insert Clathrin. This plasmid is available through Addgene.
Plasmid mEos4b-Clathrin-15 from Dr. Michael Davidsons lab contains the insert Clathrin. This plasmid is available through Addgene.
IJMS | Free Full-Text | Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid,...
Clathrin, a three-legged triskelion composed of three clathrin heavy chains (CHCs) and three light chains (CLCs), plays a critical role in clathrin-mediated endocytosis (CME) in eukaryotic cells. In this study, the genes ZmCHC1 and ZmCHC2 encoding clathrin heavy chain in maize were cloned and characterized for the first time in monocots. ZmCHC1 encodes a 1693-amino acid-protein including 29 exons and 28 introns, and ZmCHC2 encodes a 1746-amino acid-protein including 28 exons and 27 introns. The high similarities of gene structure, protein sequences and 3D models among ZmCHC1, and Arabidopsis AtCHC1 and AtCHC2 suggest their similar functions in CME. ZmCHC1 gene is predominantly expressed in maize roots instead of ubiquitous expression of ZmCHC2. Consistent with a typical predicted salicylic acid (SA)-responsive element and four predicted ABA-responsive elements (ABREs) in the promoter sequence of ZmCHC1, the expression of ZmCHC1 instead of ZmCHC2 in maize roots is significantly up-regulated by SA or ABA,
SSO mediated functional uptake in MHT cells is AP2M1 dependent and clathrin independent. (A) MHT cells were treated with 25 nM control, AP2M1, clathrin or RNA
The heterotetrameric adaptor protein complex AP2 is one of the best-studied components of the endocytic machinery. The AP2 complex consists of four different subunits, α, β2, σ2, and μ2, which assemble into a core domain with two appendages (Fig. 2; Collins et al., 2002; Jackson et al., 2010). AP2 has multiple binding partners, including phosphatidylinositol 4,5-bisphosphate (PIP2), clathrin, several endocytic accessory proteins, and two signaling motifs present on some cargo receptors (see Traub, 2009 for a detailed review). The AP2 complex has classically been considered to be the master initiator of clathrin-mediated endocytosis through its role in recruiting clathrin molecules to the membrane. However, several lines of evidence question this idea.. If the AP2 complex has an essential role in initiation then its presence would be required for the formation of endocytic sites. However, in yeast the endocytosis of mating pheromone α-factor is unaffected in strains lacking functional AP2 ...
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The nuclear export signal within clathrin assembly lymphoid myeloid leukemia protein (CALM) is critical for in vitro immortalization of cells by CALM-AF10. (a)
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mmmmm cow brains the concept centers on a particular protein called clathrin which has a unique knack for assembling itself into versatile structures that foster the formation of complex molecules clathrin is present in every cell in the human body but cows possess a vast wealth of it in their bovine brains that make them an ideal source for the stuff and given the right biochemical directions researchers think they can coax clathrin into creating better batteries and solar cells http www popsci com science article - protein-cow-brains-could-build-better-batteries-solar-cells
Thermodynamics of protein-mediated membrane deformation â application to clathrin dependent and clathrin independent endocytosis (2009 ...
Development of a Standardised Method for the Quantified Determination of Contact Lens Disinfectant Efficacy Against...
Purpose: : Although standards exist for determining the efficacy of contact lens care solutions (CLCS) against bacteria and fungi (ISO 14729) none exist for Acanthamoeba. As such, conflicting findings on the effectiveness of CLCS against the organism have been reported. Here we describe a quantifiable, reproducible and reliable method for assessing the disinfection of Acanthamoeba by CLCS. Methods: : A. castellanii Results: : After a 6 hour contact time, the log reduction in trophozoite viability for the solutions were: MPS-1 (2.5 ±0.2 n=8), MPS-2 (1.8 ±0.2 n=8), MPS-3 (0.1 ±0.1 n=3), MPS-4 (2.7 ±0.2 n=8), PER-1 (3.8 ±0.2 n=8). For the cysts the values were: MPS-1 (2.9 ±0.3 n=3), MPS-2 (2.5 ±0.2 n=3), MPS-3 (0.7 ±0.1 n=3), MPS-4 (0.2 ±0.1 n=6) and PER-1 (2.7 ±0.3 n=3). Conclusions: : The method is relatively simple to perform and enables the reproducible and quantifiable determination of CLCS efficacy against Acanthamoeba trophozoites and cysts. This will facilitate the introduction of ...
Picalm - Phosphatidylinositol-binding clathrin assembly protein - Mus musculus (Mouse) - Picalm gene & protein
Assembly protein recruiting clathrin and adapter protein complex 2 (AP2) to cell membranes at sites of coated-pit formation and clathrin-vesicle assembly. May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. Involved in AP2-dependent clathrin-mediated endocytosis at the neuromuscular junction. Plays a crucial role in fetal and adult hematopoiesis, and normal prenatal and postnatal growth and viability.
Clathrin-mediated endocytosis: what works for small, also works for big | Research - Institut Pasteur
Link to Pubmed [PMID] - 20486136. Bioessays 2010 Jun;32(6):496-504. Clathrin and the endocytosis machinery has recently been described as being required in mammalian cells for the internalization of large particles including pathogenic bacteria, fungi, and large viruses. These apparently unexpected observations, within the framework of the classical mechanisms for the formation of clathrin-coated vesicles, are now considered as examples of a new non-classical function of clathrin, which can promote the internalization of membrane domains associated to planar clathrin lattices. The role of actin downstream of clathrin seems to be critical for this still poorly characterized process. The historical frontier between endocytosis and phagocytosis is vanishing in the light of this new role for clathrin.. http://www.ncbi.nlm.nih.gov/pubmed/20486136 ...
Contribute To This Initiative. There are over one lakh children with multiple disabilities in Karnataka in the age group of 3-13 years, according to our estimates. These children suffer from severe incidences of disability like cerebral palsy, locomotor disability, and speech & hearing impairment. They are usually unable to manage their activities of daily living (ADLs) independently and have no exposure to formal learning.. APDs Community Learning Centre program has been operational since 2007. We enroll children from surrounding urban slums and low income families. At the CLCs, APDs trained staff works with these children for 2-3 years in a school-readiness curriculum. This equips them to cope with ADLs and inculcates disability and age appropriate learning skills. It is run in partnership with the community and parents/caregivers. APD has successfully transitioned 15% of children from CLCs to mainstream schools including our own Shradhanjali Integrated School. Such education empowers these ...
IJMS | Free Full-Text | New Insights into Functional Roles of the Polypyrimidine Tract-Binding Protein | HTML
Clathrin light chain B. EN. Cassette. Upstream. . FGF-R1. α. Cassette. Up- and downstream. . ... represented by the absence of the aromatic side chains typically found in RNPs (Figure 2B). ...http://www.mdpi.com/1422-0067/14/11/22906/htm
Clathrin, light chain (SPM174) | ALZFORUM
to amino acids 23-44 of Cow Clathrin light chain. Specificity. Clathrin Light Chain (CLC) ...https://www.alzforum.org/antibodies/clathrin-light-chain-spm174-0
Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans. - PubMed - NCBI
Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans.. Chen CY1, Reese ML, ... Clathrin light chain subunits (LCa and LCb) contribute to regulation of coated vesicle formation to sort proteins during ... 2. Parallel interaction at the C-termini of clathrin heavy and light chains. Plate growth and β-galactosidase (β-gal) filter ... 5. Double mutation of tryptophan to arginine at clathrin light chain LCb residues 105 and 138 abrogates the binding to HC, and ...https://www.ncbi.nlm.nih.gov/pubmed/12426379
WikiGenes - CLTA - clathrin, light chain A
Clathrin heavy and light chain isoforms originated by independent mechanisms of gene duplication during chordate evolution. ... Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB). Ponnambalam, S., Jackson ... encoding the LCa light chain maps to the long arm of chromosome 12 at 12q23-q24 and that encoding the LCb light chain (CLTB) ... In contrast, clathrin light chain genes (CLTA and CLTB) apparently arose by localized duplication, within 1-11 million years of ...https://www.wikigenes.org/e/gene/e/1211.html
Gentaur Molecular :ACR \ Clathrin light chain B \ GTX82294
Clathrin light chain B \ GTX82294 for more molecular products just contact us ... We have also other products like : Clathrin light chain B Related products : Clathrin light chain B ... WP59: Electron Transport Chain. WP772: Electron Transport Chain. WP884: Electron Transport Chain. Related Genes :. [ARP4 ... WP2434: very-long-chain-fatty-acid-biosynthesis. WP295: Electron Transport Chain. WP542: Electron Transport Chain. ...http://www.antibody-antibodies.com/product1268935-search-__Clathrin_light_chain_B__.html
Gentaur Molecular :ACR \ Clathrin light chain A \ NBP1-05035
Clathrin light chain A \ NBP1-05035 for more molecular products just contact us ... We have also other products like : Clathrin light chain A Related products : Clathrin light chain A ... WP59: Electron Transport Chain. WP772: Electron Transport Chain. WP884: Electron Transport Chain. Related Genes :. Bibliography ... WP2434: very-long-chain-fatty-acid-biosynthesis. WP295: Electron Transport Chain. WP542: Electron Transport Chain. ...http://www.antibody-antibodies.com/product1229354-search-__Clathrin_light_chain_A__.html
CLTB Gene - GeneCards | CLCB Protein | CLCB Antibody
Clathrin Light Chain B, including: function, proteins, disorders, pathways, orthologs, and expression. GeneCards - The Human ... Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. ... bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in ... Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the ...https://www.genecards.org/cgi-bin/carddisp.pl?gene=CLTB&keywords=GH05J176492&prefilter=genomic_location
Clathrin - Wikipedia
There are two forms of clathrin light chains, designated a and b. The main clathrin heavy chain, located on chromosome 17 in ... The three heavy chains provide the structural backbone of the clathrin lattice, and the three light chains are thought to ... The clathrin triskelion is composed of three clathrin heavy chains interacting at their C-termini, each ~190 kDa heavy chain ... It forms a triskelion shape composed of three clathrin heavy chains and three light chains. When the triskelia interact they ...https://en.wikipedia.org/wiki/Clathrin
The light chains of kinesin-1 are autoinhibited | PNAS
2014) Clathrin adaptors. AP2 controls clathrin polymerization with a membrane-activated switch. Science 345(6195):459-463. ... The carboxyl-terminal domain of the heavy chains is largely unstructured. The light chains associate with the heavy-chain ... We show that, like the kinesin heavy chain, the light chains of kinesin-1 exist in a dynamic conformational state that is ... Full-length, wild-type mouse kinesin light chain-2 (KLC2) and rat kinesin-1 heavy chain (KHC, Kif5C) were cloned into the ...https://www.pnas.org/content/113/9/2418?ijkey=3220d8b74f04eb89c6d342bd5d32f42021a21837&keytype2=tf_ipsecsha
Neuronal vesicle trafficking-associated protein 1 (P42857) | InterPro | EMBL-EBI
GO:0048268 clathrin coat assembly Molecular Function. GO:0032051 clathrin light chain binding ...http://www.ebi.ac.uk/interpro/protein/P42857
Frontiers | Clathrin in Chara australis: Molecular Analysis and Involvement in Charasome Degradation and Constitutive...
Analysis of Chara australis clathrin proteins revealed two heavy chains and several light chains with sequence peculiarities, ... They are known to form in response to light via secretion of trans-Golgi network (TGN) vesicles and local inhibition of ... The data obtained indicate that clathrin plays a central role not only in constitutive endocytosis but also in the degradation ... Charasome formation is reversible to allow cells to adapt to different light conditions.Here we show that darkness-induced ...https://www.frontiersin.org/articles/10.3389/fpls.2017.00020/full
Search: All Pfam signatures | InterPro | EMBL-EBI
Clathrin light chain (IPR000996) Pfam signature: PF01086 Galactose-1-phosphate uridyl transferase, N-terminal (IPR005849) Pfam ... Clathrin, heavy chain/VPS, 7-fold repeat (IPR000547) Pfam signature: PF00637 Ran binding domain (IPR000156) Pfam signature: ... Dynein light chain, type 1/2 (IPR001372) Pfam signature: PF01221 Ergosterol biosynthesis ERG4/ERG24 (IPR001171) Pfam signature ... Clathrin, heavy chain, propeller repeat (IPR022365) Pfam signature: PF01394 Pheromone/general odorant binding protein ( ...http://www.ebi.ac.uk/interpro/member-database/Pfam
Improving the photostability of bright monomeric orange and red fluorescent proteins. - PubMed - NCBI
TagRFP-T-clathrin light chain-15 (human); (l) TagRFP-T-β-actin-7 (human); (m) TagRFP-T-peroxisomes-2 (peroximal targeting ... mOrange2-Clathrin Light Chain-15 (human). (q-u) Laser scanning confocal images of HeLa cells expressing mOrange2-H2B-6 (N- ...https://www.ncbi.nlm.nih.gov/pubmed/18454154?dopt=Abstract
Association and Colocalization of Eps15 with Adaptor Protein-2 and Clathrin | JCB
1 and the heavy and light chains of clathrin that form the clathrin lattice (for review see Schmid, 1992). It has been shown ... heavy chain. LC. light chain. NH. hemagglutinin. TAPS. N-Tris[hydroxymethyl]methyl-3-amino-propanesulfonic acid buffer. ... Eps15 also associates with the EGF receptor and with the clathrin light chain (LC). The interaction of Eps15 with α-adaptin and ... Moreover, the clathrin triskelions might also be components of the Eps15-AP-2 complex, since Eps15 is present in a clathrin LC ...http://jcb.rupress.org/content/136/4/811?ijkey=a9a31085cc3fbfdbf97d63126f1a8a570f65e2a9&keytype2=tf_ipsecsha
NSG1 Gene - GeneCards | NSG1 Protein | NSG1 Antibody
GO annotations related to this gene include clathrin light chain binding. An important paralog of this gene is NSG2. ...http://www.genecards.org/cgi-bin/carddisp.pl?gene=NSG1&keywords=GH04G004496&prefilter=genomic_location
end4 - Endocytosis protein end4 - Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) - end4 gene & protein
clathrin light chain binding Source: GO_CentralInferred from biological aspect of ancestori*. "Phylogenetic-based propagation ... clathrin light chain binding Source: GO_CentralInferred from biological aspect of ancestori*. "Phylogenetic-based propagation ... clathrin adaptor activity Source: PomBase. *clathrin binding Source: GO_CentralInferred from biological aspect of ancestori*. " ... clathrin adaptor activity Source: PomBase. *clathrin binding Source: GO_CentralInferred from biological aspect of ancestori*. " ...https://www.uniprot.org/uniprot/Q9P6L5
Small Interfering RNA Profiling Reveals Key Role of Clathrin-Mediated Endocytosis and Early Endosome Formation for Infection by...
... clathrin light chain A (sc-28276), clathrin light chain B (sc-28277), dynamin I (sc-12724), dynamin II (sc-6400), PAK-1 (sc-882 ... and two clathrin light chains targeted, only the Rab5A, dynamin 3, WASF2, and clathrin light chain B siRNA pools reduced ... Together with clathrin heavy chains, the light chains form distinct structures, termed triskelions, upon which the clathrin ... Clathrin light chains A and B are the main protein coating the cytoplasmic faces of clathrin-coated pits, and they control pit ...https://jvi.asm.org/content/81/14/7786?ijkey=ffc156c5af5fa0c45030ea65c4674d7f48834ec8&keytype2=tf_ipsecsha
Dr Corinne Smith
Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly. Traffic ... Investigating the pivotal role of clathrin light chains in vesicle transport. Royal Society. 01 Sep 2012. 30 Aug 2013. ... During endocytosis a clathrin-coated vesicle is formed which contains only specifically selected cargo. The clathrin coat is ... Defining clathrin network interactions using advanced cryo-electron microscopy. Royal Society. 01 Oct 2016. 30 Sep 2017. ...https://warwick.ac.uk/fac/sci/lifesci/people/csmith
Apomorphine - DrugBank
Clathrin light chain binding. Specific Function. Interacts with clathrin light chain A and stimulates clathrin self-assembly ... and clathrin-mediated endocytosis.. Gene Name. CALY. Uniprot ID. Q9NYX4. Uniprot Name. Neuron-specific vesicular protein ...https://www.drugbank.ca/drugs/DB00714
CiNii 論文 - Imaging of plant dynamin-related proteins and clathrin around the plasma membrane...
Functional evidence for the identification of an Arabidopsis clathrin light chain polypeptide SCHEELE U ... and clathrin light chain (AtCLC) around the plasma membrane in tobacco suspension cells by using variable incidence angle ... In animal cells, dynamin and clathrin play central roles in vesicle formation in the process of endocytosis, but the roles of ... The localization pattern and behavior of the puncta were similar to those of animal dynamin and clathrin signals reported ...http://ci.nii.ac.jp/naid/10021912079
Expression of HIP1R in cancer - Summary - The Human Protein Atlas
GO:0032051 [clathrin light chain binding]. GO:0032092 [positive regulation of protein binding]. GO:0032587 [ruffle membrane]. ... GO:0030136 [clathrin-coated vesicle]. GO:0030276 [clathrin binding]. GO:0030665 [clathrin-coated vesicle membrane]. GO:0030837 ... GO:0005905 [clathrin-coated pit]. GO:0006897 [endocytosis]. GO:0006915 [apoptotic process]. GO:0030136 [clathrin-coated vesicle ... GO:0030276 [clathrin binding]. GO:0035091 [phosphatidylinositol binding]. GO:0072583 [clathrin-dependent endocytosis]. ...http://www.proteinatlas.org/ENSG00000130787-HIP1R/pathology
HIP1R - Wikipedia
Identification of a novel interaction with clathrin light chain". J. Biol. Chem. 277 (22): 19897-904. doi:10.1074/jbc. ... 2004). "Proteomic identification of brain proteins that interact with dynein light chain LC8". Proteomics. 4 (2): 339-46. doi: ... 2002). "HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. ...https://en.wikipedia.org/wiki/HIP1R
CIL:10101, Homo sapiens, epithelial cell. CIL. Dataset
Cultured retinal pigment epithelial cell were stably transfected with mCherry-clathrin light chain and EGFP-epsin. Live-cell ... This single dual color image part of an image series that contains the time lapse for the mCherry-clathrin light chain and the ... Cultured retinal pigment epithelial cell were stably transfected with mCherry-clathrin light chain and EGFP-epsin. Live-cell ...http://cellimagelibrary.org/images/10101
Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration. . Nat Commun ... We also identified a clathrin light chain, a subunit of clathrin that participates in several membrane traffic pathways [. 44. ... Dynein light chain. EgrG_000071300.1. 0. 0,02840825. -. -. Myosin essential light chain, striated adductor muscle. gi,576700259 ... Myosin regulatory light chain A, smooth adductor muscle. gi,576695936. 0,14420750. 0,11952750. 0,0027. 1,2. ...https://stacks.cdc.gov/view/cdc/34694/cdc_34694_DS13.txt
Organism | Homo sapiens
clathrin light chain Cultured retinal pigment epithelial cell were stably transfected with mCherry-clathrin light chain and ... clathrin coat Cultured retinal pigment epithelial cells immunofluorescently labeled for clathrin (green) and nucleus (blue). ...http://cellimagelibrary.org/browse/organism/Homo%20sapiens?per_page=10&page=15
- Clathrin is a large, soluble protein composed of heavy and light chains. (genecards.org)
- CLTB (Clathrin Light Chain B) is a Protein Coding gene. (genecards.org)
- In a cell, clathrin triskelion in the cytoplasm binds to an adaptor protein that has bound membrane, linking one of its three feet to the membrane at a time. (wikipedia.org)
- Immunofluorescence studies show that Eps15 colocalizes with adaptor protein-2 (AP-2) and partially with clathrin. (rupress.org)
- Colocalization studies and proteomic analyses suggest that GPR107 associates with clathrin and the retromer protein VPS35 and that GPR107 might be responsible for the return of receptors to the plasma membrane from endocytic compartments. (biologists.org)
- E. Purification and properties of a new clathrin assembly protein. (springer.com)
- Bennett, E.M., Chen, C.Y., Engqvist-Goldstein, A.E., Drubin, D.G. and Brodsky, F.M. Clathrin hub expression dissociates the actin-binding protein Hip1R from coated pits and disrupts their alignment with the actin cytoskeleton. (springer.com)
- Chen, C.Y. and Brodsky, F.M. Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo. (springer.com)
- This causes the protein clathrin to assemble into a lattice at the cytosolic side of the plasma membrane and deform the membrane into a pit. (elifesciences.org)
- The deep invagination of clathrin-coated pits that leads to fission is assisted by actin, a protein that assembles into filaments that are suggested to generate the forces needed for this process. (elifesciences.org)
- However, epsin also performs many other roles, including recruiting a membrane protein (a so-called SNARE) that directs the fate of the vesicle to the clathrin-coated pit. (elifesciences.org)
- another clathrin coat associated protein. (elifesciences.org)
- A sequential mechanism for clathrin cage disassembly by 70-kDa heat-shock cognate protein (Hsc70) and auxilin. (findaphd.com)
- Wilbur, Chen, Manalo, Hwang, Fletterick, Brodsky: Actin binding by Hip1 (huntingtin-interacting protein 1) and Hip1R (Hip1-related protein) is regulated by clathrin light chain. (antikoerper-online.de)
- Complete reconstitution of clathrin basket formation with recombinant protein fragments: adaptor control of clathrin self-assembly. (semanticscholar.org)
- Clathrin-coated pits contain an integral membrane protein that binds the AP-2 subunit with high affinity. (semanticscholar.org)
- Alexa Fluor dyes were employed to visualize distribution of clathrin and the peroxisomal membrane protein 70 ( PMP 70 ) in the culture of Madin-Darby canine kidney epithelial cells illustrated above. (fsu.edu)
- To test this hypothesis in vivo, we used evanescent-wave photobleaching recovery to study clathrin exchange from single pits using LC (LCa and LCb) and HC enhanced green fluorescent protein fusion constructs. (genes2cognition.org)
- Mechanistically related but less understood is the role of Hsp70s in the disassembly of protein complexes such as clathrin coats, viral capsids and the nucleoprotein complex, which initiates the replication of bacteriophage λ DNA. (springer.com)
- Uncoating protein (hsc70) binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis. (biovendor.com)
- Depleting both auxilins cause an approximately 50% reduction in the number of clathrin-coated pits at the plasma membrane but enhances the association of clathrin and adaptors with intracellular membranes. (biomedsearch.com)
- Dynamic events at the plasma membrane of live cells are commonly studied with light microscopy and single-particle tracking (SPT) after labeling of specific membrane components with individual fluorophores, colloidal gold, or fluorescent beads ( 1 - 3 ). (pnas.org)
- Under low-intensity BL, CRL3 NPH3 mediates the mono/multiubiquitination of phot1, likely marking it for clathrin-dependent internalization from the plasma membrane. (plantcell.org)
- Based on circular dichroism data, mapping and mutagenesis, LCa and LCb were represented as alpha-helices, aligned along the HC and, using molecular dynamics, a structural model of their interaction was generated with novel implications for LC control of clathrin assembly. (nih.gov)
- Differential control of clathrin subunit dynamics measured with EW-FRAP microscopy. (genes2cognition.org)
- Cellular control of clathrin function is thought to be aimed at the LC subunit, mainly on the basis of structural information. (genes2cognition.org)
- We conclude that HCs bound both to LCa and to LCb contribute side by side to pit formation in vivo, but the affinity of LCa-free HC in pits is reduced, and the Ca(2+)- and ATP-mediated control of clathrin function is lost. (genes2cognition.org)
- The smallest clathrin cage commonly imaged, called a mini-coat, has 12 pentagons and only two hexagons. (wikipedia.org)
- Epsin is an evolutionarily conserved endocytic clathrin adaptor whose most critical function(s) in clathrin coat dynamics remain(s) elusive. (elifesciences.org)
- WPB biogenesis at the trans‐Golgi network depends on recruitment of an AP‐1/clathrin coat to support the tubulation of VWF multimers. (bl.uk)
- Using live-cell microscopy we are exploring how signalling cargo modulates clathrin coated pit dynamics via posttranslational modifications of the clathrin coat and how this impacts on downstream signalling. (sheffield.ac.uk)
- Clathrin, a key structural element of coated pits, is composed of 190-kDa heavy chains (CHC) and 25-kDa light chains (CLC), which together form three-legged trimmers (called triskelions). (biologists.org)
- Our results indicate that a key evolutionary conserved function of epsin, in addition to other roles that include, as we show here, a low affinity interaction with SNAREs, is to help generate the force that leads to invagination and then fission of clathrin-coated pits. (elifesciences.org)
- Overall, these results suggest that a main role of epsin is to help actin interact with the clathrin-coated pits and generate the force required for a pit to develop into a vesicle. (elifesciences.org)
- We show that OCRL visits late stage endocytic clathrin coated pits and binds the Rab5 effector APPL1 on peripheral early endosomes. (pubmedcentralcanada.ca)
- After binding to the cell surface, particles typically underwent free diffusion for 5-10 s, and then they were confined in an actin filament-dependent manner without involvement of clathrin-coated pits or caveolae. (pnas.org)
- Clathrin coated pits are microdomains responsible for the uptake of a wide variety of cargo. (sheffield.ac.uk)
- In addition, the structures isolated from auxilin-depleted cells are on average smaller than CCVs from control cells and are largely devoid of membrane, indicating that they are not CCVs but membraneless clathrin cages. (biomedsearch.com)
- First, during interphase, clathrin plays a key role in membrane trafficking ( Kirchhausen, 2000 ). (biologists.org)
- Crystallographic studies reveal a role of the ASH-RhoGAP-like domains in positioning the phosphatase domain at the membrane interface and a clathrin box protruding from the RhoGAP-like domain. (pubmedcentralcanada.ca)
- CCP assembly is accompanied or followed by the progressive invagination of the membrane, eventually leading to the dynamin-dependent scission of a clathrin-coated vesicle (CCV) that delivers its cargo to the endosomal system ( 1 , 2 ). (sciencemag.org)
- This entails closure of the fusion pore and the subsequent recycling of the internalised membrane by clathrin‐mediated vesiculation. (bl.uk)
- Auxilin depletion causes self-assembly of clathrin into membraneless cages in vivo. (biomedsearch.com)
- Mutants designed to abolish trimerisation were unable to rescue the mitotic defects caused by depletion of endogenous clathrin. (biologists.org)
- Preliminary work presented here suggests loss of clathrin heavy chain or light chain B results in the formation of shorter organelles, whilst light chain A depletion promotes the biogenesis of longer granules. (bl.uk)
- LC binding to clathrin heavy chain (HC) was characterized by genetic and structural approaches. (nih.gov)
- This was originally determined from the structure of the proximal leg domain that identified and is composed of a smaller structural module referred to as clathrin heavy chain repeat motifs. (wikipedia.org)
- These results indicate that the key structural features of clathrin that are necessary for its function in mitosis are a trimeric molecule with a spindle interaction domain at each end, supporting the bridge hypothesis for clathrin function in mitosis. (biologists.org)
- We are exploiting this improvement in capability to carry out high resolution structural analysis of clathrin cage complexes. (findaphd.com)
- Structural modeling predicts that within this domain, the region of heavy chain-light chain interaction is a bundle of three or four alpha helices. (dnatube.com)
- We demonstrate that TCR internalization after receptor engagement and TCR signaling involves inducible phosphorylation of clathrin heavy chain (CHC) in both CD4 + and CD8 + human T cells. (rupress.org)
- Fernandez, McVicker, Tuma, Tuma: Ethanol selectively impairs clathrin-mediated internalization in polarized hepatic cells. (antibodies-online.com)
- Here, we describe how a previously unnoticed intramolecular interaction between the light chain tetratricopeptide repeat domain (KLC2 TPR ) and a highly conserved peptide motif within an unstructured region of the molecule occludes a key cargo binding site on the light-chain TPR domain. (pnas.org)
- The clathrin-coated pit then pinches off inside the cell in a process called fission to form a bubble-like structure called a vesicle, which transports the molecule to its destination. (elifesciences.org)
- A single CHC molecule consists of an N-terminal seven-bladed β-propeller, a linker region, eight clathrin heavy chain repeat (CHCR0-7) segments, a proximal hairpin, a tripod region that is thought to be responsible for trimerisation, and a variable C-terminal segment (residues 1631-1675). (biologists.org)
- Kinetic analysis using light scattering, fluorescence and single molecule methods. (findaphd.com)
- The clathrin molecule has a triskelion shape. (ptglab.com)
- Despite its importance for a host of cellular processes and contribution to neurological, viral, and bacterial disease, the molecular mechanisms underlying the regulation of the heterotetrameric motor kinesin-1 by its light chains and the binding of its cargo are not well understood. (pnas.org)
- Thus, like the motor-bearing kinesin heavy chains, KLCs exist in a dynamic conformational state that is regulated by self-interaction and cargo binding. (pnas.org)
- CCV fractions isolated from auxilin-depleted cells have an approximately 1.5-fold increase in clathrin content and more than fivefold increase in the amount of AP-2 adaptor complex and other endocytic machinery, with no concomitant increase in cargo. (biomedsearch.com)
- The pit grows deeper over time as more clathrin molecules assemble, eventually resulting in a deeply invaginated clathrin-coated pit that encloses the cargo to be taken up by the cell. (elifesciences.org)
- Our lab is interested in the molecular mechanisms of cargo sorting along the endocytic pathway with particular emphasis on the regulation of the clathrin coated vesicle cycle by rab5 and reversible phosphorylation. (sheffield.ac.uk)
- Clathrin light and heavy chain interface: alpha-helix binding superhelix loops via critical tryptophans. (nih.gov)
- Parallel interaction at the C-termini of clathrin heavy and light chains. (nih.gov)
- Clathrin heavy and light chain isoforms originated by independent mechanisms of gene duplication during chordate evolution. (wikigenes.org)
- Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. (genecards.org)
- The clathrin triskelion is composed of three clathrin heavy chains interacting at their C-termini , each ~190 kDa heavy chain has a ~25 kDa light chain tightly bound to it. (wikipedia.org)
- The main clathrin heavy chain, located on chromosome 17 in humans, is found in all cells. (wikipedia.org)
- A second clathrin heavy chain gene, on chromosome 22 , is expressed in muscle. (wikipedia.org)
- Clathrin heavy chain is often described as a leg, with subdomains, representing the foot (the N-terminal domain), followed by the ankle, distal leg, knee, proximal leg, and trimerization domains. (wikipedia.org)
- The light chains bind primarily to the proximal leg portion of the heavy chain with some interaction near the trimerization domain. (wikipedia.org)
- The triskelia comprise three clathrin heavy chains (CHCs) and three clathrin light chains (CLCs). (frontiersin.org)
- Clathrin is a triskelion consisting of three heavy chains each with an associated light chain. (biologists.org)
- Here, we have tested this hypothesis by replacing endogenous clathrin heavy chain in human cells with a panel of clathrin constructs. (biologists.org)
- In the present study, we aimed to test these two hypotheses by replacing endogenous clathrin heavy chain (CHC) in human cells with a variety of CHC constructs. (biologists.org)
- Novel functions of clathrin light chains: clathrin heavy chain trimerization is defective in light chain-deficient yeast. (semanticscholar.org)
- Mac?rek, Zíková, Dráberová, Dráber: Monoclonal antibody BF-06 against the heavy chain of clathrin. (antibodies-online.com)
- The clathrin heavy chain CHC-1 negatively regulates pulling forces acting on centrosomes during interphase and on spindle poles during mitosis in one-cell C. elegans embryos. (antibodies-online.com)
- Each leg consists of one heavy chain and one light chain. (embl.de)
- CHCR repeats in the clathrin heavy chain, Saccharomyces cerevisiae Vamp2 and human Vamp6 have been implicated in homooligomerization, suggesting that this may be the primary function of this repeat. (embl.de)
- Composed of three heavy chains and three light chains, clathrin exists in the shape of a triskelion, a structure with three bent legs radiating from a central point. (fsu.edu)
- 2. The method of claim 1, wherein said genes code for HLA-A201 heavy chains. (google.com)
- The present invention relates generally to the biosynthesis of temperature-sensitive major histocompatibility complex ("MHC") molecules, and more particularly relates to temperature-sensitive mutants of heavy chains of MHC class I molecules. (google.com)
- Structure-function analysis of clathrin heavy and light chains. (uhealthsystem.com)
- We are currently investigating how the molecular chaperone, Hsc70, and its cofactor auxilin, cause clathrin cage disassembly in vitro. (warwick.ac.uk)
- Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly. (findaphd.com)
- Loss of SNX-1, RME-8, or the clathrin chaperone Hsc70/HSP-1 leads to over-accumulation of endosomal clathrin, reduced clathrin dynamics, and missorting of MIG-14 to the lysosome. (antibodies-online.com)