Clathrin
The main structural coat protein of COATED VESICLES which play a key role in the intracellular transport between membranous organelles. Each molecule of clathrin consists of three light chains (CLATHRIN LIGHT CHAINS) and three heavy chains (CLATHRIN HEAVY CHAINS) that form a structure called a triskelion. Clathrin also interacts with cytoskeletal proteins.
Clathrin-Coated Vesicles
Vesicles formed when cell-membrane coated pits (COATED PITS, CELL-MEMBRANE) invaginate and pinch off. The outer surface of these vesicles is covered with a lattice-like network of the protein CLATHRIN. Shortly after formation, however, the clathrin coat is removed and the vesicles are referred to as ENDOSOMES.
Coated Pits, Cell-Membrane
Endocytosis
Brain Chemistry
Cattle
Immunoglobulin Light Chains
Myosin Light Chains
The smaller subunits of MYOSINS that bind near the head groups of MYOSIN HEAVY CHAINS. The myosin light chains have a molecular weight of about 20 KDa and there are usually one essential and one regulatory pair of light chains associated with each heavy chain. Many myosin light chains that bind calcium are considered "calmodulin-like" proteins.
Molecular Sequence Data
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Amino Acid Sequence
Protein Binding
Microscopy, Fluorescence
Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo. (1/30)
Clathrin heavy and light chains form triskelia, which assemble into polyhedral coats of membrane vesicles that mediate transport for endocytosis and organelle biogenesis. Light chain subunits regulate clathrin assembly in vitro by suppressing spontaneous self-assembly of the heavy chains. The residues that play this regulatory role are at the N terminus of a conserved 22-amino acid sequence that is shared by all vertebrate light chains. Here we show that these regulatory residues and others in the conserved sequence mediate light chain interaction with Hip1 and Hip1R. These related proteins were previously found to be enriched in clathrin-coated vesicles and to promote clathrin assembly in vitro. We demonstrate Hip1R binding preference for light chains associated with clathrin heavy chain and show that Hip1R stimulation of clathrin assembly in vitro is blocked by mutations in the conserved sequence of light chains that abolish interaction with Hip1 and Hip1R. In vivo overexpression of a fragment of clathrin light chain comprising the Hip1R-binding region affected cellular actin distribution. Together these results suggest that the roles of Hip1 and Hip1R in affecting clathrin assembly and actin distribution are mediated by their interaction with the conserved sequence of clathrin light chains. (+info)Huntingtin interacting protein 1 (HIP1) regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain. (2/30)
Huntingtin interacting protein 1 (HIP1) is a component of clathrin coats. We previously demonstrated that HIP1 promotes clathrin assembly through its central helical domain, which binds directly to clathrin light chains (CLCs). To better understand the relationship between CLC binding and clathrin assembly we sought to dissect this interaction. Using C-terminal deletion constructs of the HIP1 helical domain, we identified a region between residues 450 and 456 that is required for CLC binding. Within this region, point mutations showed the importance of residues Leu-451, Leu-452, and Arg-453. Mutants that fail to bind CLC are unable to promote clathrin assembly in vitro but still mediate HIP1 homodimerization and heterodimerization with the family member HIP12/HIP1R. Moreover, HIP1 binding to CLC is necessary for HIP1 targeting to clathrin-coated pits and clathrin-coated vesicles. Interestingly, HIP1 binds to a highly conserved region of CLC previously demonstrated to regulate clathrin assembly. These results suggest a role for HIP1/CLC interactions in the regulation of clathrin assembly. (+info)Clathrin heavy and light chain isoforms originated by independent mechanisms of gene duplication during chordate evolution. (3/30)
In humans, there are two isoforms each of clathrin heavy chain (CHC17 and CHC22) and light chain (LCa and LCb) subunits, all encoded by separate genes. CHC17 forms the ubiquitous clathrin-coated vesicles that mediate membrane traffic. CHC22 is implicated in specialized membrane organization in skeletal muscle. CHC17 is bound and regulated by LCa and LCb, whereas CHC22 does not functionally interact with either light chain. The imbalanced interactions between clathrin subunit isoforms suggest a distinct evolutionary history for each isoform pair. Phylogenetic and sequence analysis placed both heavy and light chain gene duplications during chordate evolution, 510-600 million years ago. Genes encoding CHC22 orthologues were found in several vertebrate species, with only a pseudogene present in mice. Multiple paralogons surrounding the CHC genes (CLTC and CLTD) were identified, evidence that genomic or large-scale gene duplication produced the two CHC isoforms. In contrast, clathrin light chain genes (CLTA and CLTB) apparently arose by localized duplication, within 1-11 million years of CHC gene duplication. Analysis of sequence divergence patterns suggested that structural features of the CHCs were maintained after gene duplication, but new interactions with regulatory proteins evolved for the CHC22 isoform. Thus, independent mechanisms of gene duplication expanded clathrin functions, concomitant with development of neuromuscular sophistication in chordates. (+info)Non-stoichiometric relationship between clathrin heavy and light chains revealed by quantitative comparative proteomics of clathrin-coated vesicles from brain and liver. (4/30)
We used tandem mass spectrometry with peptide counts to identify and to determine the relative levels of expression of abundant protein components of highly enriched clathrin-coated vesicles (CCVs) from rat liver. The stoichiometry of stable protein complexes including clathrin heavy chain and clathrin light chain dimers and adaptor protein (AP) heterotetramers was assessed. We detected a deficit of clathrin light chain compared with clathrin heavy chain in non-brain tissues, suggesting a level of regulation of clathrin cage formation specific to brain. The high ratio of AP-1 to AP-2 in liver CCVs is reversed compared with brain where there is more AP-2 than AP-1. Despite this, general endocytic cargo proteins were readily detected in liver but not in brain CCVs, consistent with the previous demonstration that a major function for brain CCVs is recycling synaptic vesicles. Finally we identified 21 CCV-associated proteins in liver not yet characterized in mammals. Our results further validate the peptide accounting approach, reveal new information on the properties of CCVs, and allow for the use of quantitative proteomics to compare abundant components of organelles under different experimental and pathological conditions. (+info)Membrane-associated STAT3 and PY-STAT3 in the cytoplasm. (5/30)
Signal transduction from the plasma membrane to the nucleus by STAT proteins is widely represented as exclusively a soluble cytosolic process. Using cell-fractionation methods, we observed that approximately 5% of cytoplasmic STAT3 was constitutively associated with the purified early endosome (EE) fraction in human Hep3B liver cells. By 15-30 min after interleukin-6 (IL-6) treatment, up to two-thirds of cytoplasmic Tyr-phosphorylated STAT3 can be associated with the purified early endosome fraction (Rab-5-, EEA1-, transferrin receptor-, and clathrin-positive fraction). Electron microscopy, immunofluorescence, and detergent dissection approaches confirmed the association of STAT3 and PY-STAT3 with early endosomes. STAT3 was constitutively associated with clathrin heavy chain in membrane and in the 1- to 2-MDa cytosolic complexes. The membrane association was dynamic in that, within 15 min of treatment with the vicinal-thiol cross-linker phenylarsine oxide, there was a dramatic increase in bulk STAT3 association with sedimentable membranes. The functional contribution of PY-STAT3 association with the endocytic pathway was evaluated in transient transfection assays using IL-6-inducible STAT3-reporter-luciferase constructs and selective regulators of this pathway. STAT3-transcriptional activation was inhibited by expression constructs for dominant negative dynamin K44A, epsin 2a, amphiphysin A1, and clathrin light chain but enhanced by that for the active dynamin species MxA. Taken together, these studies emphasize the contribution of the endocytic pathway to productive IL-6/STAT3 signaling. (+info)Clathrin light chain b is capable of affecting potently a major protein phosphatase from microtubules (MT-PP1). (6/30)
Clathrin light chain (CL) b purified from bovine brain postmicrotubule supernatant and identified by mass spectrometry potently inhibited a catalytic activity of a major protein phosphatase (PP) that was copurified with microtubules and recognized by antiPP1 antibodies. CLb similarly affected the catalytic subunit and holoenzyme of the PP, little inhibiting the activity of PP2A. Although the CLb from clathrin-coated vesicles was several hundredfold weaker than our purified CLb, the CLb in the postmicrotubule supernatant, independent of whether it was sedimentable or soluble, was as active as the purified CLb. Thus CLb may be a potent regulator of the PP. (+info)Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis. (7/30)
In the central nervous system, clathrin-mediated endocytosis is crucial for efficient synaptic transmission. Clathrin-coated vesicle assembly and disassembly is regulated by some 30 adaptor and accessory proteins, most of which interact with clathrin heavy chain. Using the calcyon cytosolic domain as bait, we isolated clathrin light chain in a yeast two-hybrid screen. The interaction domain was mapped to the heavy chain binding domain and C-terminal regions of light chain. Further, the addition of the calcyon C terminus stimulated clathrin self-assembly in a dose-dependent fashion. Calcyon, which is a single transmembrane protein predominantly expressed in brain, localized to vesicular compartments within pre- and postsynaptic structures. There was a high degree of overlap in the distribution of LC and calcyon in neuronal dendrites, spines, and cell bodies. Co-immunoprecipitation studies further suggested an association of calcyon with the clathrin-mediated endocytic machinery. Compared with controls, HEK293 cells overexpressing calcyon exhibited significantly enhanced transferrin uptake but equivalent levels of recycling. Conversely, transferrin uptake was largely abolished in neocortical neurons obtained from mice homozygous for a calcyon null allele, whereas recycling proceeded at wild type levels. Collectively, these data indicate a role for calcyon in clathrin-mediated endocytosis in brain. (+info)Novel function of clathrin light chain in promoting endocytic vesicle formation. (8/30)
Clathrin-mediated endocytosis is a major pathway for uptake of lipid and protein cargo at the plasma membrane. The lattices of clathrin-coated pits and vesicles are comprised of triskelions, each consisting of three oligomerized heavy chains (HC) bound by a light chain (LC). In addition to binding HC, LC interacts with members of the Hip1/R family of endocytic proteins, including the budding yeast homologue, Sla2p. Here, using in vivo analysis in yeast, we provide novel insight into the role of this interaction. We find that overexpression of LC partially restores endocytosis to cells lacking clathrin HC. This suppression is dependent on the Sla2p binding region of LC. Using live cell imaging techniques to visualize endocytic vesicle formation, we find that the N-terminal Sla2p binding region of LC promotes the progression of arrested Sla2p patches that form in the absence of HC. We propose that LC binding to Sla2p positively regulates Sla2p for efficient endocytic vesicle formation. (+info)
Gentaur Molecular :ACR \ Clathrin light chain B \ GTX101938
Publications | Bednarek Lab | Biochemistry | UW-Madison
CiNii 論文 - Imaging of plant dynamin-related proteins and clathrin around the plasma membrane...
Clathrin, light chain (SPM174) | ALZFORUM
JCI -
Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival
Microcosm Targets
CLCs experiencing spike in COVID-19 demand - Law Institute of Victoria
CLC女团组合成员信息中文网- 韩讯网 - CLC女团组合成员信息中文网
Gentaur Molecular :Exbio \ Clathrin heavy chain \ 11-526-C025
Clathrin is spindle-associated but not essential for mitosis. - Nuffield Department of Surgical Sciences
IJMS | Free Full-Text | Chloride Channelopathies of ClC-2
Tissue expression of ABCB1 - Staining in rectum - The Human Protein Atlas
Tissue expression of ABCB1 - Summary - The Human Protein Atlas
Endofin recruits clathrin to early endosomes via TOM1 | Journal of Cell Science
Functional Characterization of HIP1R in Parkinson s Disease | Parkinsons Disease
The non-canonical roles of clathrin and actin in pathogen internalization, egress and spread | Nature Reviews Microbiology
3217565 - CellBiology
Church - All Books: CLC Philippines
Addgene: mEos4a-Clathrin-15
Addgene: mEos4b-Clathrin-15
IJMS | Free Full-Text | Characterization of a Gene Encoding Clathrin Heavy Chain in Maize Up-Regulated by Salicylic Acid,...
clcs 10q.htm
Breast Feeding Resources | Philadelphia
Clathrin, heavy chain | ALZFORUM
SSO mediated functional uptake in MHT cells is AP2M1 de | Open-i
From uncertain beginnings: Initiation mechanisms of clathrin-mediated endocytosis | JCB
2016 Employment Law
Castle Triskelion: November 2015
The nuclear export signal within clathrin assembly lymp | Open-i
A tale of two CLCs: biophysical insights toward understanding ClC-5 and ClC-7 function in endosomes and lysosomes. - Department...
Western Carolinian :: North Carolina Newspaper Digitization Project
2022 Criminal Law MCLE
LetsGoPens.com • View topic - LGP Science Thread
BSN: Profile for Neeraj J Agrawal
Development of a Standardised Method for the Quantified Determination of Contact Lens Disinfectant Efficacy Against...
SLA Resume Example La Familia Pawn - Kissimmee, Florida
Picalm - Phosphatidylinositol-binding clathrin assembly protein - Mus musculus (Mouse) - Picalm gene & protein
Clathrin-mediated endocytosis: what works for small, also works for big | Research - Institut Pasteur
Community Learning Centers (CLC) | APD India
HIP1R
Identification of a novel interaction with clathrin light chain". J. Biol. Chem. 277 (22): 19897-904. doi:10.1074/jbc. ... 2004). "Proteomic identification of brain proteins that interact with dynein light chain LC8". Proteomics. 4 (2): 339-46. doi: ... 2002). "HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. ...
Frances Brodsky
"Localization of clathrin light-chain sequences mediating heavy-chain binding and coated vesicle diversity". Nature. 326 (6109 ... "Clathrin light chains' role in selective endocytosis influences antibody isotype switching". Proceedings of the National ... "A role for the CHC22 clathrin heavy-chain isoform in human glucose metabolism". Science. 324 (5931): 1192-1196. Bibcode:2009Sci ... She is known for her work on clathrin and its role in the function of the immune system. She is a professor of cell biology and ...
CLTB
Clathrin, light chain B is a protein in humans that is encoded by the CLTB gene. Clathrin is a large, soluble protein composed ... "Entrez Gene: Clathrin, light chain B". Retrieved 2013-06-05. v t e (Articles with short description, Short description matches ... This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative ... of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits ...
CALY
Xiao J, Dai R, Negyessy L, Bergson C (June 2006). "Calcyon, a novel partner of clathrin light chain, stimulates clathrin- ...
Huntingtin-interacting protein 1
HIP1's non-pathological activity includes clathrin assembly via interaction with clathrin light chains. HIP1 is the human ... regulates clathrin assembly through direct binding to the regulatory region of the clathrin light chain". The Journal of ... Actin binding by Hip-1 is altered depending on whether clathrin is also bound to Hip-1. HIP1 has also been found to be ...
Clathrin
There are two forms of clathrin light chains, designated a and b. The main clathrin heavy chain, located on chromosome 17 in ... The three heavy chains provide the structural backbone of the clathrin lattice, and the three light chains are thought to ... It forms a triskelion shape composed of three clathrin heavy chains and three light chains. When the triskelia interact they ... A second clathrin heavy chain gene, on chromosome 22, is expressed in muscle. Clathrin heavy chain is often described as a leg ...
2-hydroxy-dATP diphosphatase
"Chromosomal location and some structural features of human clathrin light-chain genes (CLTA and CLTB)". Genomics. 24 (3): 440-4 ... The enzyme is used in DNA repair, where it hydrolysis the oxidized purines and prevents their addition onto the DNA chain. ... During DNA repair, the enzyme hydrolyses oxidized purines and prevents their addition onto the DNA chain. As such it has ...
HSPA8
... binds a conformationally labile domain of clathrin light chain LCa to stimulate ATP hydrolysis". Cell. 62 (5): 875-87. doi: ... Another function of Hsc70 is as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane ... It works with auxilin to remove clathrin from coated vesicles. In neurons, synaptojanin is also an important protein involved ...
CLTC
"Analysis of clathrin light chain-heavy chain interactions using truncated mutants of rat liver light chain LCB3". J. Biol. Chem ... The basic subunit of the clathrin coat is composed of three heavy chains and three light chains. CLTC has been shown to ... Clathrin heavy chain 1 is a protein that in humans is encoded by the CLTC gene. Clathrin is a major protein component of the ... "Entrez Gene: CLTC clathrin, heavy chain (Hc)". Tebar, F; Bohlander S K; Sorkin A (Aug 1999). "Clathrin assembly lymphoid ...
Unconventional myosin-VI
October 2019). "Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension". ... "Myosin VI isoform localized to clathrin-coated vesicles with a role in clathrin-mediated endocytosis". The EMBO Journal. 20 (14 ... Myosin-VI's Met-Ile-Sec bonding interactions are limited to the myosin-VI long isoform but interact with clathrin in ... MYO6 has been shown to interact with GIPC1, DAB2., ubiquitin, and clathrin. Myosin VI, being a motor protein, focuses its ...
List of MeSH codes (D12.776.543)
... clathrin heavy chains MeSH D12.776.543.990.200.750 - clathrin light chains MeSH D12.776.543.990.300.150 - ADP-ribosylation ... cd98 heavy chain MeSH D12.776.543.585.200.374.750.500.500 - antigens, cd98 light chains MeSH D12.776.543.585.200.500 - amino ... cd98 heavy chain MeSH D12.776.543.585.200.500.500.500.500 - antigens, cd98 light chains MeSH D12.776.543.585.200.500.500.500. ... integrin alpha chains MeSH D12.776.543.750.705.408.100.100 - antigens, cd11a MeSH D12.776.543.750.705.408.100.150 - antigens, ...
AP2 adaptor complex
Dhingra A, Alexander D, Reyes-Reveles J, Sharp R, Boesze-Battaglia K (2018). "Microtubule-Associated Protein 1 Light Chain 3 ( ... These AP2 aggregates interact with individual clathrin proteins by their β-active sites, orienting them into the clathrin " ... which would serve as an important receptor group for microtubule-associated protein 1 light chain 3 (LC3). LC3 has an important ... of the clathrin trimer. AP2 facilitates the assembly of clathrin lattices when endocytosis needs to occur, by aggregating ...
MAP1A
Mann SS, Hammarback JA (1994). "Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B ... "Interactions between adaptor protein-1 of the clathrin coat and microtubules via type 1a microtubule-associated proteins". J. ... polypeptide that presumably undergoes proteolytic processing to generate the final MAP1A heavy chain and LC2 light chain. ... light chains determine distinct functional properties". J. Neurosci. 22 (6): 2106-14. doi:10.1523/JNEUROSCI.22-06-02106.2002. ...
Endocytosis
... kD protein called clathrin light chain (CLC), forming three-legged trimers called triskelions. Vesicles selectively concentrate ... The main scaffold component of clathrin coat is the 190-kD protein called clathrin heavy chain (CHC), which is associated with ... Clathrin-coated vesicles (CCVs) are found in virtually all cells and form domains of the plasma membrane termed clathrin-coated ... Clathrin-mediated endocytosis is the only pathway dependent on both clathrin and dynamin. The endocytic pathway of mammalian ...
Protein targeting
I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of ... Bulk flow COPI COPII Clathrin LocDB PSORTdb Signal peptide Nelson DL (January 2017). Lehninger principles of biochemistry. Cox ... The amino acid chain of transmembrane proteins, which often are transmembrane receptors, passes through a membrane one or ... Once in the lumen the polypeptide chain can be folded properly. This process only occurs in unfolded proteins located in the ...
Photoactivated peptide
"Light-dependent inhibition of clathrin-mediated endocytosis in yeast": 2021.04.01.432428. doi:10.1101/2021.04.01.432428. {{cite ... in the side chains or in the backbone of peptide templates to get the photo-controlled peptides, which can reversibly change ... "Light-Regulated Stapled Peptides to Inhibit Protein-Protein Interactions Involved in Clathrin-Mediated Endocytosis". Angewandte ... light. Photoswitchable peptides to inhibit protein-protein interactions in a light-controlled manner have been developed and ...
Endoplasmic reticulum
The N-terminus (one end) of a polypeptide chain (i.e., a protein) contains a few amino acids that work as an address tag, which ... The ER was observed with light microscope by Garnier in 1897, who coined the term ergastoplasm. With electron microscopy, the ... either secreted constitutively with no tag or secreted in a regulatory manner involving clathrin and paired basic amino acids ... to the side-chain nitrogen of Asn. In most cells the smooth endoplasmic reticulum (abbreviated SER) is scarce. Instead there ...
G protein-coupled receptor
... clathrin adaptor molecules); thus, the arrestin here acts as a scaffold assembling the components needed for clathrin-mediated ... Cyclic AMP-dependent protein kinases (protein kinase A) are activated by the signal chain coming from the G protein (that was ... This mechanism is used, for example, with rhodopsin in retina cells to compensate for exposure to bright light. In many cases, ... which in turn recruits another protein called clathrin. If enough receptors in the local area recruit clathrin in this manner, ...
Molecular motor
The structure of Dynein consists of A Stem Containing A region that binds to dynactin Intermediate/light chains that will ... Dynamin is responsible for the separation of clathrin buds from the plasma membrane. GTP is used. Rotary motors: FoF1-ATP ...
BUB3
Lo KW, Kogoy JM, Pfister KK (April 2007). "The DYNLT3 light chain directly links cytoplasmic dynein to a spindle checkpoint ... Cayrol C, Cougoule C, Wright M (November 2002). "The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint ...
Adenoviridae
Binding to αv integrin results in endocytosis of the virus particle via clathrin-coated pits. Attachment to αV integrin ... Such MR was demonstrated for adenovirus 12 after virions were irradiated with UV light and allowed to undergo multiple ... In the laboratory, adenovirus can be identified with antigen detection, polymerase chain reaction (PCR), virus isolation and ... "Multiplicity reactivation of human adenovirus type 12 and simian virus 40 irradiated by ultraviolet light". Virology. 45 (2): ...
SNARE (protein)
... and translocation of the light chain into the cytosol. The light chain has zinc-dependent endopeptidase or more specifically ... clathrin-coated, single-membrane vesicles containing Atg16L, the v-SNARE VAMP7, and its partner t-SNAREs: Syntaxin-7, Syntaxin- ... Finally, after the light chain of BoNT is brought into the cytosol of the targeted neuron, it is released from the heavy chain ... The light chain is released from the heavy chain by the reduction of the disulfide bond holding the two together. The reduction ...
Membrane curvature
Clathrin is involved in cellular endocytosis and is sequestrated by specific signaling molecules. Clathrin can attach to ... On the other hand, lipids with smaller acyl chain area to polar head group area ratio tend to curve positively, in other words ... Polishchuk RS, Polishchuk EV, Marra P, Alberti S, Buccione R, Luini A, Mironov AA (January 2000). "Correlative light-electron ... Clathrin binds to its receptors that are present in the membrane. C illustrates a slightly different mechanism. In this case, ...
Vinculin
Fausser JL, Ungewickell E, Ruch JV, Lesot H (October 1993). "Interaction of vinculin with the clathrin heavy chain". Journal of ... The recent discovery of the 3D structure sheds light on how this protein tailors its shape to perform a variety of functions. ...
Cytokinesis
... which activates myosin II directly by phosphorylation of the myosin light chain and also inhibits myosin phosphatase by ... large amounts of membrane material are removed via clathrin-mediated endocytosis [7] Eventually, the edges of the cell plate ... a striking process that is clearly visible through a light microscope. The cytokinetic furrow ingresses until a midbody ...
MRC Laboratory of Molecular Biology
From outside the LMB, the parent MRC ensured that the quinquennial assessment had a light touch: only a brief explanation of ... Barbara Pearse discovered the major components of clathrin-coated vesicles, structures formed during endocytosis, and a low ... J. A. Steitz, (1969) "Polypetide Chain Initiation: Nucleotide Sequences of the Three Ribosomal Binding Sites in Bacteriophage ...
Alpha-tubulin N-acetyltransferase
Tubulin Acetylation Protein Isoform Post-translational modification Microtubules Clathrin Portal: Biology (EC 2.3.1, Enzymes of ... Isoform 4 Isoform 4 is different to the canonical sequence as the sequence of amino acids 323-333 from the canonical chain ( ... it might play an important paper in the intracilial transport of signal proteins during light-sensing signaling in ... or the clathrin coated-pit in the membrane. This is closely related to one of its main functions which is the catalysis of ...
Alcohol intoxication
These include the ceremony of lighting candles which stay lit during the Sabbath day, eating hot food from a fire lit ... "A Molecular Target for an Alcohol Chain-Length Cutoff". Journal of Molecular Biology. 431 (2): 196-209. doi:10.1016/j.jmb. ... Problematic alcohol use associates with sodium channel and clathrin linker 1 (SCLT1) in trauma-exposed populations Addict Biol; ... "News Lite". Daily News of Los Angeles. 23 July 1999. Retrieved 19 October 2022. Bales, Robert F. "Attitudes toward Drinking in ...
Hippocalcin
MLK2 is myosin light chain kinase 2 The b2 adaptin of the AP2 complex Calcium-dependent activator protein for secretion (CADPS ... clathrin and tubulin". Biochem. J. 363 (Pt 3): 599-608. doi:10.1042/0264-6021:3630599. PMC 1222513. PMID 11964161. O'Callaghan ...
V-ATPase
Reverse transcription polymerase chain reaction studies have shown expression of the a4 subunit in the intercalated cell of the ... A relatively new technique called ancestral gene resurrection has shed new light on the evolutionary history of the V-ATPase. ... Forgac M (January 1999). "The vacuolar H+-ATPase of clathrin-coated vesicles is reversibly inhibited by S-nitrosoglutathione". ...
Apoptosis
The tumor-suppressor protein p53 accumulates when DNA is damaged due to a chain of biochemical factors. Part of this pathway ... Apoptosis (from Ancient Greek: ἀπόπτωσις, romanized: apóptōsis, lit. ''falling off'') is a form of programmed cell death that ... December 2008). "Oropouche virus entry into HeLa cells involves clathrin and requires endosomal acidification". Virus Research ...
Oxford-AstraZeneca COVID-19 vaccine
"WHO backs AstraZeneca COVID vaccine amid clotting concerns; green lights Johnson & Johnson shots". UN News. 12 March 2021. ... "SARS coronavirus entry into host cells through a novel clathrin- and caveolae-independent endocytic pathway". Cell Research. 18 ... January 2021 after a controversy that the price each country paid depended on production cost and differences in supply chain, ... heterologous prime-boost vaccination course consisting of one dose of the Oxford-AstraZeneca vaccine followed by Sputnik Light ...
Papillomaviridae
Studies have shown that the crystal of isolated L1 capsomeres has the heparin chains recognized by lysines lines grooves on the ... This classification may need revision in the light of the existence of papilloma-polyoma virus recombinants. Additional species ... The virion is internalized, probably through a clathrin-mediated process, into an endosome, where acidic conditions are thought ... Day PM, Lowy DR, Schiller JT (March 2003). "Papillomaviruses infect cells via a clathrin-dependent pathway". Virology. 307 (1 ...
Human papillomavirus infection
By binding both proteins, E6 induces E6AP to attach a chain of ubiquitin molecules to p53, thereby flagging p53 for proteosomal ... in vitro studies suggest that the E6 protein of the HPV virus may inhibit apoptosis induced by ultraviolet light. Nearly all ... and annexin A2 leading to entry of the virions into basal epithelial cells through clathrin-mediated endocytosis and/or ...
HIV
Zhang C, Zhou S, Groppelli E, Pellegrino P, Williams I, Borrow P, Chain BM, Jolly C (2015). "Hybrid Spreading Mechanisms and T ... But urban migration rose, increasing sexual contacts and leading to red-light districts. Wertheim JO, Worobey M (May 2009). " ... More recently, however, productive infection by pH-independent, clathrin-mediated endocytosis of HIV-1 has also been reported ... a polymerase chain reaction (PCR), western blot or, less commonly, an immunofluorescence assay (IFA)). Only specimens that are ...
Short linear motif
A Dynein Light Chain binding motif in Rabies virus is vital for host infection). The extent of human SLiM mimicry is surprising ... November 2004). "Evolving nature of the AP2 alpha-appendage hub during clathrin-coated vesicle endocytosis". The EMBO Journal. ...
CLTB Protein Human | Clathrin Light Chain B | ProSpec
... non-glycosylated polypeptide chain containing 234 amino acids and having a molecular mass of 25.6kDa ... Clathrin light chain B, Lcb, CLTB.. Introduction. Clathrin light chain B (CLTB) is a member of the clathrin light chain family ... CLTB forms a triskelion form comprised of 3 clathrin heavy chains and 3 light chains. When the triskelia interact they form a ... Clathrin is a protein that has a key role in the formation of coated vesicles. CLTB functions as the key structural component ...
Results for 'Vesicle Transport' | Abcam: antibodies, proteins, kits...
Plus it
Xiao J, Dai R, Negyessy L, Bergson C (2006) Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated ... 3A-C; lanes marked IP: IgG). Heavy and light chains of control IgG are present similar to heavy chain in Figure 3A,B; lowest ... while NSG3 binds clathrin light chain (CLC), the AP1-3 adaptors, and PSD-95 (Xiao et al., 2006; Ha et al., 2012; Muthusamy et ... heavy chain and light chains from antibodies used for pull down were detected in most Co-IP conditions (all IP lanes). ...
Spirochrome Probes for Bioimaging. SiR-actin and SiR-tubulin offered by Cytoskeleton, Inc.
A unique role for clathrin light chain A in cell spreading and migration. Journal of Cell Science. 2019. ISSN 1477-9137. ... SiR probes are excited by and emit light in the near infrared/far red spectral range, thus avoiding the use of shorter ... A8: No-under the very high light intensities typically used in STORM imaging, a phototoxic effect is observed on live cells. ... the capture of images with a higher resolution than conventional light microscopy which is constrained by diffraction of light ...
Background
Clathrin light chains are required for the gyrating-clathrin recycling pathway and thereby promote cell migration. . Nat Commun ... We also identified a clathrin light chain, a subunit of clathrin that participates in several membrane traffic pathways [. 44. ... Dynein light chain. EgrG_000071300.1. 0. 0,02840825. -. -. Myosin essential light chain, striated adductor muscle. gi,576700259 ... Myosin regulatory light chain A, smooth adductor muscle. gi,576695936. 0,14420750. 0,11952750. 0,0027. 1,2. ...
A syntaxin 10-SNARE complex distinguishes two distinct transport routes from endosomes to the trans-Golgi in human cells |...
Clathrin heavy and light chain isoforms originated by independent mechanisms of gene duplication during chordate evolution. ... Clathrin heavy and light chain isoforms originated by independent mechanisms of gene duplication during chordate evolution. ... The missorting of MPRs in cells depleted of STX10 was confirmed by light microscopy. As shown in Fig. 4 A, cells containing ... Endosome to Golgi transport of ricin is independent of clathrin and of the Rab9- and Rab11-GTPases. Mol. Biol. Cell. ...
WANG LMO4 TARGETS DN
DeCS
The light chain subunits of clathrin. Preferred term. Clathrin Light Chains Entry term(s). Clathrin Light Chain Light Chains, ... Clathrin Light Chains - Preferred Concept UI. M0404383. Scope note. ... The light chain subunits of clathrin.. Allowable Qualifiers:. AD administration & dosage. AE adverse effects. AG agonists. AI ... Clathrin Light Chain. Light Chains, Clathrin. Tree number(s):. D12.776.543.990.200.750. ...
Browse by Funders - WRAP: Warwick Research Archive Portal
2013) Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly. ... 2015) A novel disorder reveals clathrin heavy chain-22 is essential for human pain and touch development. Brain, 138 (8). pp. ... 2014) A new role for clathrin adaptor proteins 1 and 3 in lipoplex trafficking. PLoS ONE, Volume 9 (Number 3). Article number ... 2011) A sequential mechanism for clathrin cage disassembly by 70-kDa heat-shock cognate protein (Hsc70) and auxilin. National ...
IMP: Integrative Multi-species Prediction
DAS-431 IV: Uses, Interactions, Mechanism of Action | DrugBank Online
Publications
Human CLTA ELISA Kit | biobool.com
TomoSeq data search
Hemípteros/microbiología
... phytoplasmas were observed very closed to recombinant GFP-labelled clathrin light chain. To verify the role of clathrin in the ... Chlorpromazine, cytochalasin D and knockdown of clathrin heavy chain (chc) gene expression using RNA interference inhibited ... Clathrin-mediated endocytosis is used by a wide range of bacteria to infect eukaryote cells. Among the insect proteins ... In conclusion, results indicate that clathrin is important for the FD phytoplasma to enter insect cells and colonize its insect ...
CIL advanced search
MacKay Lab - Intracellular Switches
To test this hypothesis, we fused ELPs to clathrin-light chain (CLC), a protein associated with clathrin-mediated endocytosis. ... Keywords Synthetic biology, elastin-like polypeptide, inverse phase transition temperature, clathrin-light chain, clathrin- ... In 2014, our lab reported that temperature mediated assembly of ELP-Clathrin light chain (CLC) fusion proteins can be employed ... Flipping the Switch on Clathrin-Mediated Endocytosis using Thermally Responsive Protein Microdomains. Martha K. Pastuszka, ...
A09
AIM 2: To investigate how clathrin light chains (CLCs) participate in polarized sorting and establish- ment of polarity ... we have also identified that clath- rin light chains a and b (CLCa/b) play a key role in mediating the polarized sorting of ... Our results from the first funding period shows that the clathrin machinery (AP-1 and CLCa/b) drives the establishment of an ... In the second funding period, we aim at identifying the molecular mechanisms by which the clathrin/AP-1 machinery drive the ...
Gene O00443 | Protein PIK3C2A - Overview | canSAR.ai
Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation ... Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably ... Interacts with clathrin trimers (PubMed:11239472, PubMed:16215232). Interacts with SBF2/MTMR13 (By similarity). ...
Items where Subject is "Alternative Splicing" - CSHL Scientific Digital Repository
YOR184W 2167.216480 INESSENTIAL SER1 phosphoserine transaminase,phosphoserine aminotransferase
2.549529 INESSENTIAL CLC1 Clathrin light chain YIL025C -2.549816 INESSENTIAL biological_process unknown, molecular_function ... 2.871007 INESSENTIAL DYN2 putative light chain of dynein, microtubule-based process*, microtubule motor, cell YJR130C 2.870417 ... YBR288C 130.526942 INESSENTIAL APM3 clathrin associated protein medium chain YML022W 129.757172 INESSENTIAL APT1 Adenine ... 0.780449 INESSENTIAL FAA4 long-chain fatty acid--CoA ligase and synthetase 4,long-chain-fatty-acid-CoA-ligase, YDL134C 0.779367 ...
Crosstalk of ancient pathways : Phosphoinositides link receptor-triggered MAP-kinase signalling to membrane trafficking in...
... the adaptor complex starts to recruit clathrin-heavy chain (CHC) and clathrin-light chain (CLC) subunits from the cytosol to ... Besides others, clathrin-mediated endocytosis (CME) is the most prominent and best studied endocytotic pathway in eukaryotes ( ... In this image, the acyl chains are represented by linoleic acid (at C3 position) and linolenic acid (at the C3 position). B) ... As plants furthermore are the basis of every food chain, to understand how crop plants respond to environmental changes is of ...
JCI -
Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival
... clathrin heavy chain (clathrin), and adaptin-γ, a subunit of the clathrin-associated adaptor protein AP1. nl, normal prostate ... In light of the proposed involvement of the htt/HIP1 pathway in cell survival and apoptosis, it is interesting to note that ... HIP1 functions in clathrin-mediated endocytosis through binding to clathrin and adaptor protein 2. J Biol Chem 2001. 276:39271- ... Simultaneous binding of PtdIns(4, 5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes. Science 2001 ...
Pharmaceuticals | Free Full-Text | Elucidating the Function of Penetratin and a Static Magnetic Field in Cellular Uptake of...
... known to selectively block clathrin formation) blocking particle uptake. Interestingly, it was further shown that a magnetic ... Clathrin- medicated endocytosis appeared to be responsible for uptake as shown via PCR and western blot, with Pitstop 2 ( ... 2.6.1. Clathrin and Caveolin Real Time Polymerase Chain Reaction (q-rtPCR). Cells were as described on a 24 well plate and ... The hydrodynamic diameter of the mNPs was as described by Chemicell (~ 200 nm), which was confirmed via dynamic light ...
WikiGenes - TFAP2A - transcription factor AP-2 alpha...
Clathrin assembly protein AP-2 is detected in both neurons and glia, and its reduction is prominent in layer II of frontal ... Sleep apnea impairs the arousal response to airway occlusion. Berry, R.B., Kouchi, K.G., Der, D.E., Dickel, M.J., Light, R.W. ... A region from the medium chain adaptor subunit (mu) recognizes leucine- and tyrosine-based sorting signals. Bremnes, T., ... c-Src regulates clathrin adapter protein 2 interaction with beta-arrestin and the angiotensin II type 1 receptor during ...
ACTB and HIP1R - Wiki-MPM
Difference between revisions of "Biology" - Master Projects
clathrin coated vesicles (cargo receptor, adaptin, clathrin, dynamin), rab-tethering protein, t/v. ... cristae electron transport chain NADH dehydrogenase, ubiquinone, cytochrome b-c1 complex, cytochrome c, cytochrome oxidase ... light harvesting complex(antenna complex), reaction centre carbon fixation cycle / Calvin cycle Rubisco ... endocytosis exocytosis cis/trans golgi network clathrin coated vesicles (cargo receptor, adaptin, clathrin, dynamin), rab- ...
YRC Public Data Repository - Protein Structure Prediction - Protein OverviewEndocytosisProteinsVesiclePolypeptideAdaptorProteinRecombinantInteractsPathwayCLCAAssemblyCellularHeavy chainSyntheticRoleFamilyCellsComplexPhaseLongStudyProteinsVesiclesMammalian cellsDynaminRole of clathrinEndosomesVesicle biogenesisMembraneCargoEndogenousEukaryotic cellsInternalizationTriskelionActin filaments2001HeavyTriskeliaAdaptorsColocalizationLatticeVitroAccumulationSequenceMoleculeSubunitsElectron microscopyAntibodiesTemporal and spatialBindDistinctFluorescentFunction
Endocytosis11
- CME (Clathrin-mediated endocytosis) controls numerous cellular physiological processes such as the internalization of growth factors and receptors, entry of pathogens, and synaptic transmission. (prospecbio.com)
- Interacts with clathrin light chain A and stimulates clathrin self-assembly and clathrin-mediated endocytosis. (drugbank.com)
- Clathrin-mediated endocytosis is used by a wide range of bacteria to infect eukaryote cells. (bvsalud.org)
- Among the insect proteins interacting with the phytoplasma adhesin VmpA, we identified the adaptor protein complex AP-1 and AP-2 suggesting that phytoplasmas could enter the insect cells via clathrin-mediated endocytosis. (bvsalud.org)
- In 2014, our lab reported that temperature mediated assembly of ELP-Clathrin light chain (CLC) fusion proteins can be employed to reversibly inhibit Clathrin Mediated Endocytosis (Pastuszka et al. (mackaylab.com)
- To test this hypothesis, we fused ELPs to clathrin-light chain (CLC), a protein associated with clathrin-mediated endocytosis. (mackaylab.com)
- Prior to thermal stimulation, the ELP fusion is soluble and clathrin-mediated endocytosis remains 'on. (mackaylab.com)
- Increasing the temperature induces the assembly of ELP fusion proteins into organelle-sized microdomains that switches clathrin-mediated endocytosis 'off. (mackaylab.com)
- These microdomains can be thermally activated and inactivated within minutes, are reversible, do not require exogenous chemical stimulation, and are specific for components trafficked within the clathrin-mediated endocytosis pathway. (mackaylab.com)
- which function as sorting adapters for G protein-coupled receptors (GPCRs) in clathrin-mediated endocytosis, raising the possibility that it may function in the selection of integral membrane proteins for export. (elifesciences.org)
- Epsins belong to the family of highly conserved clathrin-associated sorting proteins that are indispensable for clathrin-mediated endocytosis, but their precise functions remain unclear. (sigmaaldrich.com)
Proteins3
- By exploiting proteomic approaches with pro- ject A05 [Bruegger (HD)/Freund (B)] and project A01 [Nickel (HD)/Ewers (B)], we will perform the first in depth characterization of the proteins that are apically and basolaterally sorted by the clathrin/AP-1 machinery in human IECs. (uni-heidelberg.de)
- Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
- Neue Bilder proteins in electrophilic DNA download Democracy in the Arab World: Explaining the Deficit (Routledge Studies desaturase( MMR) overhangs( MLH1, PMS2, MSH2, and MSH6) are involved by stress p53 and been retardation during complex and car-light accommodations. (familie-vos.de)
Vesicle2
Polypeptide1
- CLTB Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 234 amino acids (1-211 a.a) and having a molecular mass of 25.6kDa (Molecular size on SDS-PAGE will appear higher). (prospecbio.com)
Adaptor1
- We identified that the clathrin sorting adaptor protein AP-1 was responsible for sorting TLR3 to the basolateral side of hIECs. (uni-heidelberg.de)
Protein1
- Clathrin is a protein that has a key role in the formation of coated vesicles. (prospecbio.com)
Recombinant2
- During invasion of S2 cells, phytoplasmas were observed very closed to recombinant GFP-labelled clathrin light chain. (bvsalud.org)
- Because recombinant antibody production involves sequencing the antibody light and heavy chains, it is a highly controlled and reliable process. (cellsignal.com)
Interacts1
- Interacts with clathrin trimers (PubMed:11239472, PubMed:16215232). (cansar.ai)
Pathway1
- That clathrin-coated pits in E. histolytica trophozoites endocytose ferritin, which is then destroyed in the endosome/lysosome pathway by particular cysteine proteases [7]. (romj.org)
CLCA2
- 2019). Importantly, we have also identified that clath- rin light chains a and b (CLCa/b) play a key role in mediating the polarized sorting of TLRs in IECs. (uni-heidelberg.de)
- Our results from the first funding period shows that the clathrin machinery (AP-1 and CLCa/b) drives the establishment of an immune gradient within the cell allowing for the partial tolerance of the gut microbial content while remaining fully immune-responsive against invasive pathogens or during microbial infiltration associated with the loss of epithelium barrier function. (uni-heidelberg.de)
Assembly1
- Spatial Control of Epsin-induced Clathrin Assembly by Membrane Curvature. (sigmaaldrich.com)
Cellular1
- In the second funding period, we aim at identifying the molecular mechanisms by which the clathrin/AP-1 machinery drive the establishment of cellular polarity, in general, and how they mediate the spatial segregation of TLR3 to the basolateral side of IECs. (uni-heidelberg.de)
Heavy chain1
- Chlorpromazine, cytochalasin D and knockdown of clathrin heavy chain (chc) gene expression using RNA interference inhibited entry of phytoplasmas into S2 cells. (bvsalud.org)
Synthetic1
- A self-organized synthetic morphogenic liposome responds with shape changes to local light cues. (mpg.de)
Role1
- To verify the role of clathrin in the insect colonization by phytoplasmas, RNAi was performed via artificial feeding of chc dsRNA by the vector E. variegatus. (bvsalud.org)
Family1
- Clathrin light chain B (CLTB) is a member of the clathrin light chain family. (prospecbio.com)
Cells1
- In conclusion, results indicate that clathrin is important for the FD phytoplasma to enter insect cells and colonize its insect vector. (bvsalud.org)
Complex1
- Thus, MPRs may travel from early endosomes to recycling endosomes to late endosomes en route to the Golgi complex. (rupress.org)
Phase1
- A new approach for the synthesis of long alkyl chain length stationary phases for use in reversed-phase liquid chromatography is described. (sigmaaldrich.com)
Long1
- acyl-CoA synthetase long chain f. (gsea-msigdb.org)
Study1
- The aim of this study was to develop a low shrinkage visible light curable nanocomposite dental restorative material without sacrificing the other properties of conventional materials. (sigmaaldrich.com)
Proteins12
- These findings illustrate the importance of clathrin-mediated recycling of slit-diaphragm proteins during podocyte health and disease. (asn-online.org)
- 6 It has been shown previously that proteins within the clathrin-mediated endocytic pathway, such as dynamin, synaptojanin1, and endophilin, are critical to normal kidney function, because mice lacking these genes developed massive albuminuria. (asnjournals.org)
- Interestingly, many of the proteins that were identified by mass spectrometry have trafficking-related functions and include the clathrin light chain B and Sec23A, an ER to Golgi trafficking vesicle coat component. (edu.au)
- Clathrin also interacts with cytoskeletal proteins. (bvsalud.org)
- The subunits that make up the large, medium and small chains of adaptor proteins. (lookformedical.com)
- A subclass of clathrin assembly proteins that occur as monomers. (lookformedical.com)
- Single chains of amino acids that are the units of multimeric PROTEINS. (lookformedical.com)
- The ligands that bind and activate these receptors include light-sensitive compounds, odors , pheromones , hormones , and neurotransmitters , and vary in size from small molecules to peptides to large proteins . (wikipedia.org)
- The BTN1 liver is then the regulatory responsible telophase urban aldehyde, whereas the BTN2 and BTN3 proteins each are three chains BTN2A1, BTN2A2 and BTN2A3, and BTN3A1, BTN3A2 and BTN3A3, n't( complex that BTN2A3 causes a cytokine). (erik-mill.de)
- Cytoskeletal proteins such as dynamin (see IPR001401 ), Caenorhabditis elegans kinesin-like protein unc-104 (see IPR001752 ), spectrin beta-chain, syntrophin (2 PH domains) and S. cerevisiae nuclear migration protein NUM1. (embl.de)
- clathrin-coated cell identified to make assembled or to provide at the addition project( PM) hyper-activates the menbrane via such degree proteins. (erik-mill.de)
- Two conserved scaffold proteins, clathrin and Ede1 (Eps15), also arrive during the early phase. (stencila.io)
Vesicles6
- Deletion of Synaptojanin 1 (Synj1), which is involved in the uncoating of clathrin-coated vesicles (CCV), resulted in severe albuminuria and foot process effacement in mice. (asn-online.org)
- An adaptor protein complex primarily involved in the formation of clathrin-related endocytotic vesicles (ENDOSOMES) at the CELL MEMBRANE. (lookformedical.com)
- https://doi.org/10.1083/jcb.202109013 ) take an innovative approach to directly demonstrate the indispensable functions of both clathrin and its adaptor network in shaping endocytic vesicles. (rupress.org)
- The rigidity and inherent curvature of the clathrin lattice has led to the long-standing assumption that assembly of clathrin provides a major driving force for the curvature of endocytic vesicles. (rupress.org)
- However, electron microscopy cannot follow the morphological changes that occur during development of individual clathrin-coated vesicles. (rupress.org)
- Using nano-fabricated substrates with highly curved ridges, the research team examined the impact of substrate curvature on the dynamics and cargo content of clathrin-coated vesicles. (rupress.org)
Mammalian cells3
- pmKate2-clathrin vector can be transfected into mammalian cells by any known transfection method. (evrogen.com)
- In mammalian cells, clathrin has two functions. (biologists.com)
- First, they reported the dynamic exchange of clathrin during assembly of endocytic sites in mammalian cells. (rupress.org)
Dynamin2
- Fractional recovery analysis of the immunoprecipitated CaV2.2 complex by exposure to high NaCl (∼1 m) indicated that AP180 and S-intersectin adaptors are tightly bound to CaV2.2 while L-intersectin, H- and L-clathrin and dynamin form a less tightly linked subcomplex. (nyu.edu)
- The above 3D kymograph of the cell surface, with the time dimension in the z-axis, shows the full lifetime of hundreds of clathrin patches on the membrane, which terminate upon recruitment of dynamin. (berkeley.edu)
Role of clathrin1
- We comprehensively explore the role of clathrin in AP-3-dependent vesicle budding, using rapid chemical-genetic perturbation of clathrin function with a clathrin light chain-FKBP chimera oligomerizable by the drug AP20187. (nih.gov)
Endosomes2
- [ 32 ] Lysis of the endosomes leads to escape and the cytosolic Ad is then transported along the microtubule cytoskeleton, as a result of Ad5 hexon directly binding to dynein intermediate and light chains, and travels towards the perinuclear microtubule organizing centers. (medscape.com)
- We find that AP-3 interacts and colocalizes with endogenous and recombinant FKBP chimeric clathrin polypeptides in PC12-cell endosomes. (nih.gov)
Vesicle biogenesis4
- Chemical-genetic disruption of clathrin function spares adaptor complex 3-dependent endosome vesicle biogenesis. (nih.gov)
- A role for clathrin in AP-3-dependent vesicle biogenesis has been inferred from biochemical interactions and colocalization between this adaptor and clathrin. (nih.gov)
- These findings indicate that AP-3-clathrin association is dispensable for endosomal AP-3 vesicle budding and suggest that endosomal AP-3-clathrin interactions differ from those by which AP-1 and AP-2 adaptors productively engage clathrin in vesicle biogenesis. (nih.gov)
- This gap was partially addressed by Willy and Kural who, in a 2021 paper, used super-resolution microscopy to analyze changes in the shape and area of the clathrin coat during vesicle biogenesis ( 7 ). (rupress.org)
Membrane8
- First, during interphase, clathrin plays a key role in membrane trafficking ( Kirchhausen, 2000 ). (biologists.com)
- They perform functions such as binding to the cell membrane, capturing cargo molecules and promoting the assembly of CLATHRIN. (lookformedical.com)
- However, a fundamental problem has dogged this hypothesis since its inception: namely, that clathrin does not bind directly to membrane surfaces. (rupress.org)
- Over the past decade, several groups have presented new data on clathrin dynamics, which have alternately strengthened and weakened the case for clathrin's role in generating membrane curvature. (rupress.org)
- If a flat and rapidly exchanging clathrin lattice is recruited to endocytic sites, how can clathrin be a strong driver of membrane bending? (rupress.org)
- May be required to determine the amount of membrane to be recycled, possibly by regulating the size of the clathrin cage. (uniprot.org)
- After internalization, a fraction of this membrane is recycled back to the plasma membrane in an Arf6-dependent manner, and another fraction of this membrane fuses with membrane internalized via the clathrin pathway. (nih.gov)
- Thus, this Arf6-associated endosomal membrane system parallels and intersects with the clathrin pathway. (nih.gov)
Cargo1
- Divergent modes for cargo-mediated control of clathrin-coated pit dynamics. (berkeley.edu)
Endogenous2
- Here, we have tested this hypothesis by replacing endogenous clathrin heavy chain in human cells with a panel of clathrin constructs. (biologists.com)
- Mutants designed to abolish trimerisation were unable to rescue the mitotic defects caused by depletion of endogenous clathrin. (biologists.com)
Eukaryotic cells2
- CMV promoter provides strong, constitutive expression of the mKate2-clathrin fusion in eukaryotic cells. (evrogen.com)
- 1 However, expression in the cytoplasm or nucleus of eukaryotic cells of full-length immunoglobulin G or single-chain antibody variable domain fragments aiming at blocking an intracellular target is poorly efficient because of the reducing nature of these compartments. (ashpublications.org)
Internalization1
- We employed two-colour total internal reflection microscopy to study the recruitment and dynamics of various components of the actin polymerization machinery and the epidermal growth factor receptor signalling machinery during clathrin-coated pit internalization in control cells and cells genetically deficient for functional N-WASP. (biologists.com)
Triskelion2
- Clathrin is a triskelion consisting of three heavy chains each with an associated light chain. (biologists.com)
- Each molecule of clathrin consists of three light chains ( CLATHRIN LIGHT CHAINS ) and three heavy chains ( CLATHRIN HEAVY CHAINS ) that form a structure called a triskelion. (bvsalud.org)
Actin filaments2
- 4. Regulation of Hip1r by epsin controls the temporal and spatial coupling of actin filaments to clathrin-coated pits. (nih.gov)
- Recent work demonstrated that clathrin-coated pit invagination can coincide with assembly of actin filaments and with accumulation of N-WASP and Arp2/3 complex, but the relevance of their recruitment has remained poorly defined. (biologists.com)
20011
- HN - 2001 MH - Amino Acids, Neutral UI - D021542 MN - D12.125.154 MS - Amino acids with uncharged R groups or side chains. (nih.gov)
Heavy11
- We noted that CaV2.2 clusters abut heavy-chain (H)-clathrin patches at the transmitter release face. (nyu.edu)
- A single CHC molecule consists of an N-terminal seven-bladed β-propeller, a linker region, eight clathrin heavy chain repeat (CHCR0-7) segments, a proximal hairpin, a tripod region that is thought to be responsible for trimerisation, and a variable C-terminal segment (residues 1631-1675). (biologists.com)
- Subunit of the MHC class I peptide loading complex (GO:0042824) (=PLC) involved in the assembly of the heavy-chain-beta2-microglobulin dimers of the MHC class I molecules that fold with eight to ten residue peptides in the endoplasmic reticulum. (beds.ac.uk)
- S-1 contains the heads of the heavy chains plus the light chains and S-2 contains part of the double-stranded, alpha-helical, heavy chain tail (myosin rod). (lookformedical.com)
- Two heavy and two pairs of light chains comprise this conventional myosin (now known as myosin II), which polymerizes into filaments to interact with actin and generate force through the hydrolysis of ATP. (nih.gov)
- The Laboratory of Cell Biology is led by Dr. Edward Korn, who has been studying the function and regulation of the actomyosin system in its diverse forms since he discovered the first unconventional non-filamentous myosin, myosin I (containing only a single heavy chain), in the single-cell soil protozoan Acanthamoeba castellanii , approximately forty years ago. (nih.gov)
- Commercially-available goat-anti-dog IgG-heavy and light chain horseradish peroxidase conjugate (Bethyl Laboratories, USA) was used as the secondary antibody, in the suggested dilution of 1 1:60,000. (monossabios.com)
- The 5'-terminal nucleotide sequence of some molecules of heavy strand mitochondrial DNA is known to consist of a short segment of RNA that is complimentary to a short light strand mitochondrial DNA sequence nearby. (nih.gov)
- Hence, NG-10 cDNA may correspond to an RNA transcript of the light strand of mitochondrial DNA that serves as a primer for the initiation of heavy strand mitochondrial DNA synthesis. (nih.gov)
- These results show that treatment of NG108-15 neuroblastoma-glioma cells with dibutyryl cAMP results in marked increases in the abundance of RNA transcripts from heavy and light strands of mitochondrial DNA. (nih.gov)
- We found that silencing 29 genes reduced focus size, and only one ((clathrin heavy chain) and (membraneCcytoskeletal BIO-1211 linker ezrin), did not affect cell-to-cell spread in our screen (Table 1 and Supplemental Table S1). (bioscience2006.org)
Triskelia1
- we showed that normal mitosis could be rescued by full-length clathrin triskelia and not by the N-terminal domain alone, but this did not allow us to distinguish between these two models. (biologists.com)
Adaptors1
- Does clathrin drive curvature directly or is it simply organizing curvature-driving adaptors? (rupress.org)
Colocalization1
- They find a striking colocalization of clathrin-coated pits with the highly curved regions of their substrates. (rupress.org)
Lattice3
- An alternative view is that clathrin does not act as a bridge, but as a lattice or matrix that can support spindle fibres. (biologists.com)
- The clathrin coat, which was first characterized more than 40 years ago, consists of a curved lattice of hexagons and pentagons ( 1 ). (rupress.org)
- Additionally, using correlative light-electron microscopy, they found that clathrin initially assembled as a flat lattice at endocytic sites, making an abrupt transition to high curvature, while largely conserving the area of the lattice. (rupress.org)
Vitro1
- Using co-immunoprecipitation and GST-binding assays the association between HAP1 and clathrin light chain B has been validated in vitro. (edu.au)
Accumulation1
- Actin accumulation at clathrin-coated pits requires the Arp2/3 complex, since Arp2/3 complex sequestration in the cytosol abolished any detectable actin assembly. (biologists.com)
Sequence2
- To increase mRNA translation efficiency, Kozak consensus translation initiation site is generated upstream of the mKate2-clathrin coding sequence [Kozak, 1987]. (evrogen.com)
- pmKate2-clathrin vector can be used as a source of mKate2-clathrin hybrid sequence. (evrogen.com)
Molecule1
- These results indicate that the key structural features of clathrin that are necessary for its function in mitosis are a trimeric molecule with a spindle interaction domain at each end, supporting the bridge hypothesis for clathrin function in mitosis. (biologists.com)
Subunits1
- The light chain subunits of clathrin. (nih.gov)
Electron microscopy1
- Photonic-chip assisted correlative light and electron microscopy. (embl.org)
Antibodies1
- Immunofluorescence (IF) staining with ZO-1 and clathrin light-chain antibodies on wild-type and Synj1 KO mouse podocytes was performed to determine ZO-1 localization. (asn-online.org)
Temporal and spatial1
- We analyzed through live imaging, the temporal and spatial relationship between ZO-1 and clathrin in isolated control podocytes. (asn-online.org)
Bind1
- As the N-terminal domain at the foot of each leg can bind to the mitotic spindle, we proposed previously a `bridge hypothesis' wherein clathrin acts as a brace between two or three microtubules within a kinetochore fibre to increase fibre stability. (biologists.com)
Distinct1
- With FRET-CLEM, we measure conformational changes in clathrin at thousands of individual morphologically distinct clathrin-coated structures. (nih.gov)
Fluorescent1
- The vector can be used for fluorescent labeling of clathrin LCB in living cells. (evrogen.com)
Function3
- AP-3 cofractionates with clathrin-coated vesicle fractions isolated from PC12 cells even after clathrin function is acutely inhibited by AP20187. (nih.gov)
- AP-3 vesicle formation continued, however, after brefeldin A wash-out despite impairment of clathrin function by AP20187. (nih.gov)
- In this study, a proteomics approach was used for the identification of novel HAP1-interacting partners to attempt to shed light on the physiological function of HAP1. (edu.au)
