A species of the PESTIVIRUS genus causing exceedingly contagious and fatal hemorrhagic disease of swine.
An acute, highly contagious disease affecting swine of all ages and caused by the CLASSICAL SWINE FEVER VIRUS. It has a sudden onset with high morbidity and mortality.
The lone species of the genus Asfivirus. It infects domestic and wild pigs, warthogs, and bushpigs. Disease is endemic in domestic swine in many African countries and Sardinia. Soft ticks of the genus Ornithodoros are also infected and act as vectors.
Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).
A sometimes fatal ASFIVIRUS infection of pigs, characterized by fever, cough, diarrhea, hemorrhagic lymph nodes, and edema of the gallbladder. It is transmitted between domestic swine by direct contact, ingestion of infected meat, or fomites, or mechanically by biting flies or soft ticks (genus Ornithodoros).
A genus of FLAVIVIRIDAE, also known as mucosal disease virus group, which is not arthropod-borne. Transmission is by direct and indirect contact, and by transplacental and congenital transmission. Species include BORDER DISEASE VIRUS, bovine viral diarrhea virus (DIARRHEA VIRUS, BOVINE VIRAL), and CLASSICAL SWINE FEVER VIRUS.
A species of PESTIVIRUS causing a congenital sheep disease characterized by an abnormally hairy birth-coat, tremors, and poor growth.
A family of large icosahedral DNA viruses infecting insects and poikilothermic vertebrates. Genera include IRIDOVIRUS; RANAVIRUS; Chloriridovirus; Megalocytivirus; and Lymphocystivirus.
Infections with viruses of the genus PESTIVIRUS, family FLAVIVIRIDAE.
The type species of the FLAVIVIRUS genus. Principal vector transmission to humans is by AEDES spp. mosquitoes.
Ribonucleic acid that makes up the genetic material of viruses.
Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.
A mosquito-borne species of the PHLEBOVIRUS genus found in eastern, central, and southern Africa, producing massive hepatitis, abortion, and death in sheep, goats, cattle, and other animals. It also has caused disease in humans.
A group of viruses in the genus PESTIVIRUS, causing diarrhea, fever, oral ulcerations, hemorrhagic syndrome, and various necrotic lesions among cattle and other domestic animals. The two species (genotypes), BVDV-1 and BVDV-2 , exhibit antigenic and pathological differences. The historical designation, BVDV, consisted of both (then unrecognized) genotypes.
Layers of protein which surround the capsid in animal viruses with tubular nucleocapsids. The envelope consists of an inner layer of lipids and virus specified proteins also called membrane or matrix proteins. The outer layer consists of one or more types of morphological subunits called peplomers which project from the viral envelope; this layer always consists of glycoproteins.
A species of SWINE, in the family Suidae, comprising a number of subspecies including the domestic pig Sus scrofa domestica.
The degree of pathogenicity within a group or species of microorganisms or viruses as indicated by case fatality rates and/or the ability of the organism to invade the tissues of the host. The pathogenic capacity of an organism is determined by its VIRULENCE FACTORS.
A round-to-oval mass of lymphoid tissue embedded in the lateral wall of the PHARYNX. There is one on each side of the oropharynx in the fauces between the anterior and posterior pillars of the SOFT PALATE.
Established cell cultures that have the potential to propagate indefinitely.
Proteins encoded by a VIRAL GENOME that are produced in the organisms they infect, but not packaged into the VIRUS PARTICLES. Some of these proteins may play roles within the infected cell during VIRUS REPLICATION or act in regulation of virus replication or VIRUS ASSEMBLY.
Proteins found in any species of virus.
Viral proteins that are components of the mature assembled VIRUS PARTICLES. They may include nucleocapsid core proteins (gag proteins), enzymes packaged within the virus particle (pol proteins), and membrane components (env proteins). These do not include the proteins encoded in the VIRAL GENOME that are produced in infected cells but which are not packaged in the mature virus particle,i.e. the so called non-structural proteins (VIRAL NONSTRUCTURAL PROTEINS).
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Diseases of domestic swine and of the wild boar of the genus Sus.
Immunoglobulins produced in response to VIRAL ANTIGENS.
A CELL LINE derived from the kidney of the African green (vervet) monkey, (CERCOPITHECUS AETHIOPS) used primarily in virus replication studies and plaque assays.
The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.
Visible morphologic changes in cells infected with viruses. It includes shutdown of cellular RNA and protein synthesis, cell fusion, release of lysosomal enzymes, changes in cell membrane permeability, diffuse changes in intracellular structures, presence of viral inclusion bodies, and chromosomal aberrations. It excludes malignant transformation, which is CELL TRANSFORMATION, VIRAL. Viral cytopathogenic effects provide a valuable method for identifying and classifying the infecting viruses.
Substances elaborated by viruses that have antigenic activity.
A species of NAIROVIRUS of the family BUNYAVIRIDAE. It is primarily transmitted by ticks and causes a severe, often fatal disease in humans.
An abnormal elevation of body temperature, usually as a result of a pathologic process.
Antibodies that reduce or abolish some biological activity of a soluble antigen or infectious agent, usually a virus.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
A species of CERCOPITHECUS containing three subspecies: C. tantalus, C. pygerythrus, and C. sabeus. They are found in the forests and savannah of Africa. The African green monkey (C. pygerythrus) is the natural host of SIMIAN IMMUNODEFICIENCY VIRUS and is used in AIDS research.
The presence of viruses in the blood.
The complete genetic complement contained in a DNA or RNA molecule in a virus.
An acute infectious disease primarily of the tropics, caused by a virus and transmitted to man by mosquitoes of the genera Aedes and Haemagogus. The severe form is characterized by fever, HEMOLYTIC JAUNDICE, and renal damage.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Live vaccines prepared from microorganisms which have undergone physical adaptation (e.g., by radiation or temperature conditioning) or serial passage in laboratory animal hosts or infected tissue/cell cultures, in order to produce avirulent mutant strains capable of inducing protective immunity.
The interactions between a host and a pathogen, usually resulting in disease.
An acute infection caused by the RIFT VALLEY FEVER VIRUS, an RNA arthropod-borne virus, affecting domestic animals and humans. In animals, symptoms include HEPATITIS; abortion (ABORTION, VETERINARY); and DEATH. In humans, symptoms range from those of a flu-like disease to hemorrhagic fever, ENCEPHALITIS, or BLINDNESS.
A genus of softbacked TICKS, in the family ARGASIDAE, serving as the vector of BORRELIA, causing RELAPSING FEVER, and of the AFRICAN SWINE FEVER VIRUS.
A subclass of PEPTIDE HYDROLASES that catalyze the internal cleavage of PEPTIDES or PROTEINS.
A variation of the PCR technique in which cDNA is made from RNA via reverse transcription. The resultant cDNA is then amplified using standard PCR protocols.
A family of double-stranded DNA viruses containing one genus Asfivirus. It is the source of AFRICAN SWINE FEVER.
Animals considered to be wild or feral or not adapted for domestic use. It does not include wild animals in zoos for which ANIMALS, ZOO is available.
A quantitative measure of the frequency on average with which articles in a journal have been cited in a given period of time.
Venous vessels in the umbilical cord. They carry oxygenated, nutrient-rich blood from the mother to the FETUS via the PLACENTA. In humans, there is normally one umbilical vein.
Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.

Localization of pestiviral envelope proteins E(rns) and E2 at the cell surface and on isolated particles. (1/236)

The glycoproteins E(rns) of classical swine fever virus (CSFV) and E(rns) and E2 of bovine viral diarrhoea virus (BVDV) are shown to be located at the surface of infected cells by the use of indirect immunofluorescence and by cytofluorometric analysis. The positive immunostaining of the cell surface was further analysed by immunogold electron microscopy and it could be shown that only extracellular virions were labelled. Gold granules were not seen at the cellular plasma membrane. In contrast to BVDV E2, the CSFV E2 of virions sticking to the plasma membrane was not accessible to the respective monoclonal antibodies. However, CSFV particles isolated from culture supernatant were able to bind both monoclonal anti-E(rns) and anti-E2 antibodies. For CSFV and BVDV, binding of anti-E(rns) antibodies to the virions was more pronounced than that of anti-E2. This finding was unexpected since E2 is considered to be the immunodominant glycoprotein.  (+info)

Cytopathogenic and noncytopathogenic RNA replicons of classical swine fever virus. (2/236)

To determine the minimal requirements for autonomous RNA replication of classical swine fever virus (CSFV), genomes having in-frame deletions within the genes for structural and flanking nonstructural proteins were constructed, based on an infectious cDNA clone of CSFV Alfort/187. RNA was transcribed in vitro from the respective plasmids and transfected into SK-6 swine kidney cells. The replication competence of the RNA was determined by immunostaining transfected cells for CSFV NS3 protein and by analysis of cell extracts for viral RNA, as well as protein synthesis at different times after transfection. The genes encoding N(pro), C, E(rns), E1, E2, p7, and NS2 proved to be dispensable for RNA replication, but the efficiency of replication varied strongly between individual constructs. RNA replicons containing the complete NS2-NS3 gene persisted in transfected cells and continued to replicate without causing any obvious morphological or functional damage to the cells, whereas genomes lacking the NS2 gene replicated more efficiently and induced a cytopathic effect. These findings suggest that NS2, although it is not essential for pestivirus RNA replication, has a regulatory function therein. Both cytopathogenic and noncytopathogenic replicons were packaged into virus particles provided in trans by a cotransfected full-length helper virus genome.  (+info)

Classical swine fever in Sardinia: epidemiology of recent outbreaks. (3/236)

A variable region of the gene encoding the major glycoprotein (E2) of Classical Swine Fever Virus (CSFV) was sequenced from 12 Sardinian isolates which had been obtained from three geographically distinct regions of the Island. Phylogenetic analysis of these viruses and others characterized in previous studies [1, 2] indicated that (a) the Sardinian viruses were all members of the common European subgroup 2.3 and were clearly distinct from live vaccines recently used in this area; (b) they could be resolved into four distinct groups in accordance with the region or date of isolation; (c) in at least two regions wild boar/domestic swine contact was implicated in virus spread; (d) the oldest isolate (1983) and some of the recent isolates were possibly introduced from mainland Italy. In addition, this study has wider implications for the interpretation of CSFV variation. We have been able to demonstrate that small variations within this region of the virus genome (possibly less than 2.7% or five nucleotide substitutions) can be used to separate isolates into groups that precisely fit their geographical distribution. This finding is especially important for deducing the epidemiological relationships between multiple outbreaks caused by similar viruses that occur in close proximity.  (+info)

Mutations abrogating the RNase activity in glycoprotein E(rns) of the pestivirus classical swine fever virus lead to virus attenuation. (4/236)

Classical swine fever (CSF) is a severe hemorrhagic disease of swine caused by the pestivirus CSF virus (CSFV). Amino acid exchanges or deletions introduced by site-directed mutagenesis into the putative active site of the RNase residing in the glycoprotein E(rns) of CSFV abolished the enzymatic activity of this protein, as demonstrated with an RNase test suitable for detection of the enzymatic activity in crude cell extracts. Incorporation of the altered sequences into an infectious CSFV clone resulted in recovery of viable viruses upon RNA transfection, except for a variant displaying a deletion of the histidine codon at position 297 of the long open reading frame. These RNase-negative virus mutants displayed growth characteristics in tissue culture that were undistinguishable from wild-type virus and were stable for at least seven passages. In contrast to animals inoculated with an RNase-positive control virus, infection of piglets with an RNase-negative mutant containing a deletion of the histidine codon 346 of the open reading frame did not lead to CSF. Neither fever nor extended viremia could be detected. Animals infected with this mutant did not show decrease of peripheral B cells, a characteristic feature of CSF in swine. Animal experiments with four other mutants with either exchanges of codons 297 or 346 or double exchanges of both codons 297 and 346 showed that all these RNase-negative mutants were attenuated. All viruses with mutations affecting codon 346 were completely apathogenic, whereas those containing only changes of codon 297 consistently induced clinical symptoms for several days, followed by sudden recovery. Analyses of reisolated viruses gave no indication for the presence of revertants in the infected animals.  (+info)

The RNA-dependent RNA polymerases of different members of the family Flaviviridae exhibit similar properties in vitro. (5/236)

The virus-encoded RNA-dependent RNA polymerase (RdRp), which is required for replication of the positive-strand RNA genome, is a key enzyme of members of the virus family Flaviviridae. By using heterologously expressed proteins, we demonstrate that the 77 kDa NS5B protein of two pestiviruses, bovine viral diarrhoea virus and classical swine fever virus, and the 100 kDa NS5 protein of the West Nile flavivirus possess RdRp activity in vitro. As originally shown for the RdRp of hepatitis C virus, RNA synthesis catalysed by the pestivirus and flavivirus enzymes is strictly primer-dependent in vitro. Accordingly, initiation of RNA polymerization on homopolymeric RNAs and heteropolymeric templates, the latter with a blocked 3'-hydroxyl group, was found to be dependent on the presence of complementary oligonucleotide primer molecules. On unblocked heteropolymeric templates, including authentic viral RNAs, the RdRps were shown to initiate RNA synthesis via intramolecular priming at the 3'-hydroxyl group of the template and 'copy-back' transcription, thus yielding RNase-resistant hairpin molecules. Taken together, the RdRps of different members of the Flaviviridae were demonstrated to exhibit a common reactivity profile in vitro, typical of nucleic acid-polymerizing enzymes.  (+info)

Classical swine fever virus E(rns) deletion mutants: trans-complementation and potential use as nontransmissible, modified, live-attenuated marker vaccines. (6/236)

An SK6 cell line (SK6c26) which constitutively expressed the glycoprotein E(rns) of classical swine fever virus (CSFV) was used to rescue CSFV E(rns) deletion mutants based on the infectious copy of CSFV strain C. The biochemical properties of E(rns) from this cell line were indistinguishable from those of CSFV E(rns). Two E(rns) deletion mutants were constructed, virus Flc23 and virus Flc22. Virus Flc23 encoded only the utmost N- and C-terminal amino acids of E(rns) (deletion of 215 amino acids) to retain the original protease cleavage sites. Virus Flc22 is not recognized by a panel of E(rns) antibodies, due to a deletion of 66 amino acids in E(rns). The E(rns) deletion mutants Flc22 and Flc23 could be rescued in vitro only on the complementing SK6c26 cells. These rescued viruses could infect and replicate in SK6 cells but did not yield infectious virus. Virus neutralization by E(rns)-specific antibodies was similar for the wild-type virus and the recombinant viruses, indicating that E(rns) from SK6c26 cells was incorporated in the viral particles. Pigs vaccinated with Flc22 or Flc23 were protected against a challenge with a lethal dose of CSFV strain Brescia. This is the first demonstration of trans-complementation of defective pestivirus RNA with a pestiviral structural protein and opens new ways to develop nontransmissible modified live pestivirus vaccines. In addition, the absence of (the antigenic part of) E(rns) in the recombinant viral particles can be used to differentiate between infected and vaccinated animals.  (+info)

Pathogenesis of granulocytopenia and bone marrow atrophy during classical swine fever involves apoptosis and necrosis of uninfected cells. (7/236)

Granulocytopenia, a hematological hallmark of classical swine fever, is partially responsible for the suppression of innate immune defenses during classical swine fever. The present report demonstrates that this depletion was apparent as early as 3 days postinfection (p.i.). Both mature peripheral and bone marrow neutrophils were affected, whereas immature neutrophils increased absolutely in the periphery and coincidentally immature myeloid progenitors in the bone marrow. These data suggest that a pathogenic relationship exists between these compartments. The central event was not the arrest of hematopoietic cell proliferation or of the mobilization process, but instead apoptosis and possibly also necrosis were shown to play a role. This increase in apoptotic and dead cells was detected as early as 1-3 days p.i. In contrast, viral RNA in bone marrow hematopoietic cells (BMHC) was first detected 5 days p.i., and significant amounts of infected BMHC were detected only 7 days p.i., with the major target being the myeloid compartment. The increased caspase-3 activity observed supported a role for apoptotic cell death. Furthermore, the elevated caspase-9 activity indicated the involvement of the mitochondrial apoptotic pathway. Taken together, the results demonstrate that granulocytopenia and bone marrow atrophy are mediated by hematopoietic cell death and that indirect virus-host-mediated mechanisms are likely to be responsible.  (+info)

Ultrastructural glomerular changes in experimental infection with the classical swine fever virus. (8/236)

Ultrastructural studies of glomerular changes were performed on 16 pigs experimentally infected with a highly virulent strain of the classical swine fever virus. Our observations revealed the thickening of glomerular basement membranes, swelling of endothelial cells and cytoplasmic vacuolization within podocytes containing abundant viral particles. An early viral infection of podocytes was suggested as the cause of selective swelling of the foot processes of these cells with the consequent obliteration of the glomerular urinary spaces. To our knowledge this is the first report of ultrastructural evidence of classical swine fever virions infecting glomerular podocytes.  (+info)

Classical swine fever virus (CSFV) infection causes significant losses of pigs, which is characterized by hemorrhage, disseminated intravascular coagulation and leucopenia. The swine vascular endothelial cell is a primary target cell for CSFV. The aim of this study was to determine the role of CSFV infection in inducing oxidative stress (OS) in vascular endothelial cells. We demonstrated that CSFV infection induced oxidative stress in swine umbilical vein endothelial cells (SUVECs), characterized by the induction of reactive oxygen species (ROS) production and the elevations of porcine antioxidant proteins thioredoxin (Trx), peroxiredoxin-6 (PRDX-6) and heme oxygenase-1 (HO-1) expression. Furthermore, cyclooxygenase-2 (COX-2), a pro-inflammatory protein related to oxidative stress, was up-regulated while anti-inflammatory protein peroxisome proliferator-activated receptor-γ (PPAR-γ), an important mediator in vascular functional regulation, was down-regulated in the CSFV infected cells. In addition,
Graham, SP, Everett, HE, Haines, FJ, Johns, HL, Sosan, OA, Salguero, FJ, Clifford, DJ, Steinbach, F, Drew, TW and Crooke, HR (2012) Challenge of Pigs with Classical Swine Fever Viruses after C-Strain Vaccination Reveals Remarkably Rapid Protection and Insights into Early Immunity ...
Read Induction of immune responses in mice and pigs by oral administration of classical swine fever virus E2 protein expressed in rice calli, Archives of Virology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Read Pathway analysis in blood cells of pigs infected with classical swine fever virus: comparison of pigs that develop a chronic form of infection or recover, Archives of Virology on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Classical swine fever (CSF) is a highly contagious swine disease caused by classical swine fever virus (CSFV). Previously, we demonstrated that rAdV-SFV-E2, an adenovirus-delivered, Semliki Forest virus replicon-vectored marker vaccine against CSF, is able to protect pigs against lethal CSFV challenge. From an economical point of view, it will be beneficial to reduce the minimum effective dose of the vaccine. This study was designed to test the adjuvant effects of Salmonella enteritidis-derived bacterial ghosts (BG) to enhance the protective immunity of rAdV-SFV-E2 in pigs. Groups of 5-week-old pigs (n = 4) were immunized intramuscularly twice with 105 median tissue culture infective doses (TCID50) rAdV-SFV-E2 combined with 1010 colony forming units (CFU) BG, 106 or 105 TCID50 rAdV-SFV-E2 alone or 1010 CFU BG alone at an interval of 3 weeks, and challenged with the highly virulent CSFV Shimen strain at 1 week post-booster immunization. The results show that the pigs inoculated with 105 TCID50 rAdV-SFV
The early identification of classical swine fever epizootics is hampered by difficulties in recognising early signs of infection, due to a lack of specific clinical signs. In addition many textbook descriptions of CSF are based on observations of disease caused by historic, mainly genotype 1, strains. Our objective was to improve our knowledge of the diverse range of signs that different CSFV strains can cause by characterising the experimental infection of domestic pigs with both a recent strain of CSFV and a divergent strain. Conventional pigs were inoculated with a genotype 2.1 isolate, that caused an outbreak in the UK in 2000, and a genotype 3.3 strain that is genetically divergent from European strains. This latter strain is also antigenically distinct as it is only poorly recognised by the CSFV-specific monoclonal antibody, WH303. Transmission was monitored by use of in-contact animals. Clinical, virological and haematological parameters were observed and an extended macro- and ...
A surveillance program directed specifically at detecting Classical Swine Fever is now in progress at Iowa State University Veterinary Diagnostic Laboratory (ISU VDL). The ISU VDL is one of the 12 core laboratories in the National Animal Health Laboratory Network (NAHLN). The primary function of NAHLN labs is to participate in ongoing surveillance and response to foreign animal diseases and other events requiring an integrated laboratory effort of preparedness, controlled testing, and timely reporting. Federal funding for this program provides equipment and diagnostic reagents as well as support for technical positions and proficiency training of technicians. The ISU VDL has the in-house capability to test for Classical Swine Fever, High Pathogenic Avian Influenza and Exotic Newcastle Disease.. Diagnosticians at the ISU VDL will select suspect cases from routine submissions to the ISU VDL. The criteria listed below will be used in case selection.. ...
Classical swine fever virus (CSFV) -specific cytotoxic T lymphocytes (CTL) were derived from peripheral blood mononuclear leukocytes of immunized NIH-minipigs (MHC d/d haplotype) after in vitro restimulation with infectious CSFV. Their cytotoxic activity was determined against CSFV-infected target cells obtained from simian virus 40 (SV40) large T antigen-transfected immortalized kidney cells of a syngeneic miniature swine. Experiments with separated effector cell populations revealed that the CSFV-specific cytotoxic activity was mediated by CD4-CD6+CD8+ MHC class I-restricted T lymphocytes. Infection of target cells with various vaccinia virus/CSFV recombinants led to the identification of a major antigenic site for CSFV-specific CTL near the cleavage site between the non-structural proteins p80 (NS3) and p10 (NS4a). Using synthetic overlapping nonapeptides which covered this protein region the sequence ENALLVALF is the first sequence to be identified as an MHC class I-restricted T cell epitope
inproceedings{377967, author = {Dewulf, Jeroen and Laevens, Hans and KOENEN, F and Mintiens, Koen and de Kruif, Aart}, booktitle = {of the OIE International Conference on the Control of Infectious Animal Diseases by Vaccination}, title = {The use of conventional and marker vaccines in the control of classical swine fever epidemics}, year = {2004 ...
At his regular weekly round-up meeting with stakeholders, the Governments Chief Veterinary Officer, Jim Scudamore, emphasised the importance and reasons behind the current control measures against the outbreak of classical swine fever in East Anglia. Noting the recent reductions in the size of current infected areas, and the Commissions decision to limit the ban on exports of live pigs to Norfolk, Suffolk, and Essex, he said: Classical swine fever is a virulent disease which, once established, can be difficult to control.
Outbreaks of classical swine fever (CSF) can lead to large economic losses and have a major impact on animal welfare. A pig infected by CSF virus (CSFV) may show specific clinical symptoms (conjunctivitis, skin haemorrhages, cyanotic ears and lameness), but often the signs are aspecific (fever, dullness, diarrhoea and loss of appetite; Klinkenberg et al. 2005). Meanwhile, the infection can spread rapidly to other pigs (Laevens et al. 1998) and to other farms. At the moment the disease is first detected, a large number of farms may already be infected. To halt the epidemic, reducing the number of susceptible animals in the affected area is often required, especially in pig-dense regions. Two methods to achieve this are pre-emptive culling and emergency vaccination. Pre-emptive culling was used in the later stages of the 1997/1998 epidemic of CSF in The Netherlands, causing a large number of animals to be destroyed or prematurely slaughtered. This and subsequent experiences with foot-and-mouth ...
Zdravilo Porcilis Pesti so preučevali v okviru dveh terenskih študij varnosti, eni na pitancih in eni na svinjah, pri čemer sta bila uporabljena različna odmerka cepiva. Terenske študije potrjujejo rezultate laboratorijskih preizkusov varnosti in kažejo, da je izdelek varen za ciljne živali (pujski od 5 meseca dalje) in najobčutljivejšo kategorijo živali (breje svinje).. Glavno merilo učinkovitosti pri terenskih študijah s pujski je bilo preživetje pujskov, če so bili izpostavljeni virusu KPK pozneje v življenju. Pujski so bili testirani tudi za viremijo (prisotnost virusa v krvi). Začetek in trajanje imunosti so merili na podlagi protiteles proti antigenu (E2) virusa KPK, ki so jih tvorili pujski. Maternalno pridobljena protitelesa niso vplivala na rezultate cepljenja.. ...
Classical Swine Fever (CSF) is an endemic disease in Cuba, and an eradication strategy by zones is planned by the Official Veterinary Service. The aim of this study was to identify high-risk areas of CSF and the risk factors associated with the disease occurrence in the Pinar del Río province, one of the prioritized areas in the eradication strategy. The outbreak occurrence at district level was analyzed through a 7-year period (2009 to 2015). A high-risk cluster (RR = 5.13, 95% CI 3.49-7.56) was detected during the last 2 years of the study period in the eastern half of the province, with 38 out of 97 districts included. The rate of CSF-affected holdings had a significant increase during 2014-2015 and seems to have occurred mainly in the high-risk cluster area. Swine population density by district (heads/km2) and road length (km) by district were associated with the disease outbreak occurrence. These results provide new insights into the knowledge of the epidemiology of the disease in Cuban endemic
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Accepted name: pestivirus NS3 polyprotein peptidase. Reaction: Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1 of the NS4A-NS4B cleavage site, whereas serine is found at position P1 of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites. Other name(s): border disease virus NS3 endopeptidase; BDV NS3 endopeptidase; bovine viral diarrhea virus NS3 endopeptidase; BVDV NS3 endopeptidase; classical swine fever virus NS3 endopeptidase; CSFV NS3 endopeptidase; p80. Comments: The polyprotein of noncytopathogenic pestiviruses is cleaved co- and post-translationally into at least 11 proteins (Npro, C, Erns, E1, E2, p7, NS2-3, NS4A, NS4B, NS5A, and NS5B) [2]. The genomes of cytopathogenic pestivirus strains express at least one additional protein, called NS3 (p80) [2]. This enzyme, which resides in the N-terminal region of NS3 (nonstructural protein 3), is essential for generation of its own C-terminus and for processing of the downstream cleavage sites, ...
2008) Classical swine fever virus vaccine stability in Lao PDR. In: Conlan, J., Blacksell, S., Morrissy, C. and Colling, A., (eds.) Management of classical swine fever and foot-and-mouth disease in Lao PDR : proceedings of an international workshop held in Vientiane, Lao PDR, 20-21 November 2006. ACIAR Proceedings No. 128. ACIAR, Canberra, Australia, pp. 80-83. Morrissy, C., Wright, L., Conlan, J., Goff, W., Colling, A., Hammond, J., Johnson, M., Blacksell, S. and Daniels, P. ...
hybridization patterns, the distinct genotypes of CSFV Brecent years. It can detect different pathogens in a could be differentiated. The results showed that the piece of chip and can differentiate the various CSFV amplified by primer CP5.6 could be classified into genotypes in the same species. In 2003, a bio-chip three genotypes, including the native, invaded, and diagnosis system was established by using classical lapinized hog cholera vaccine virus by hybridization swine fever virus (CSFV) as a model. Nucleotide with oligonucleotide probe. No cross reaction was sequences of classical swine fever virus were collected found when the bio-chip was hybridized with bovine from the GenBank, and the domestic CSFV field strains diarrhea disease virus which was closely related to the were sequenced. These sequence data were analyzed with a computer software by sequence alignment and apair of universal primers, which could amplify different News source: National Veterinary Research Insti- ...
Baroness Hayman today attended a meeting between senior MAFF officials and representatives of all industries affected by the CSF outbreak. The Minister of State had been invited to the meeting at Harleston, Norfolk, which provided a further opportunity to discuss face-to-face with those affected the practical welfare and financial problems being experienced in infected areas. Speaking after the meeting Baroness Hayman said: Classical swine fever is a highly contagious pig disease which can have a devastating impact on businesses and families, whether through the disease itself or consequential movement restrictions. I have come to East Anglia again today to listen directly to those affected by this outbreak. A variety of issues were raised which will be considered both at the control centre in Bury St Edmunds and in London. Controlling this disease remains of paramount importance to farmers and the wider industry. It is our first priority. We have been aware for some time of the potential welfare
The objective of this study was to describe oral fluid and serum antibody (IgG, IgA) responses against classical swine fever virus (CSFV) E2 and Erns proteins in pigs (n = 60) inoculated with a moderately virulent field strain (ALD, n = 30) or a modified live virus vaccine strain (LOM, n = 30). Oral fluid (n = 1391) and serum (n = 591) samples were collected from individually-housed pigs between d ...
Genetic labelling of viruses with a fluorophore allows to study their life cycle in real time, without the need for fixation or staining techniques. Within the family Flaviviridae, options for genetic labelling of non-structural proteins exist. Yet, no system to genetically label structural proteins has been put forward to date. Taking advantage of a previously described site within the structural protein E2, a fluorophore was introduced into a cytopathogenic (cpe) BVDV-1 virus (BVDVE2_fluo). This insertion was well tolerated, resulting in a 2-fold drop in titer compared to the parental virus, and remained stably integrated into the genome for more than 10 passages. The fluorophore E2 fusion protein was readily detectable in purified virus particles by Western blot and fluorescence microscopy and the particle integrity and morphology was confirmed by cryo electron microscopy. The same integration site could also be used to label the related Classical swine fever virus. Also, BVDVE2_fluo particles bound
No diagnostic sample submission is complete without including the tonsil. Because of their location in the oropharynx, the tonsils are exposed to a variety of viral and bacterial pathogens. Advances in diagnostic capabilities have made analysis of the tonsils an increasingly important tool in diagnosing a number of endemic swine diseases. United States Department of Agriculture (USDA) Animal and Plant Health Inspection Services (APHIS) now has funding to use the tonsil as part of a routine surveillance program to detect classical swine fever (CSF) and is offering incentives to encourage practitioners to submit samples for surveillance.. Tests using the tonsil have been developed by the Foreign Animal Disease Diagnostic Laboratory (FADDL) at USDAs Plum Island Animal Disease Center to aid in detection and diagnosis of CSF. USDAs Classical Swine Fever (CSF) Surveillance Procedure Manual1 includes tonsil, tonsil scrapings, and nasal swabs as appropriate samples for CSF detection if collected and ...
This video covers vaccines and diagnostic tests for avian influenza and is designed for those who will select vaccines and diagnostic tests to be used in an H5N1 avian influenza control program ...
Merck & Co., Inc., Kenilworth, NJ, USA is a global healthcare leader working to help the world be well. From developing new therapies that treat and prevent disease to helping people in need, we are committed to improving health and well-being around the world. The Merck Veterinary Manual was first published in 1955 as a service to the community. The legacy of this great resource continues as the Merck Veterinary Manual in the US and Canada and the MSD Manual outside of North America.. ...
TY - ABST. T1 - Virulence in pigs of vPader10 rescued from an infectious cDNA clone of the CSFV strain Paderborn. AU - Friis,Martin Barfred. AU - Nielsen,Jens. AU - Uttenthal,Åse. AU - Belsham,Graham. AU - Rasmussen,Thomas Bruun. PY - 2012. Y1 - 2012. N2 - The BAC clone, pBeloPader10, contains a complete cDNA of the CSFV strain Paderborn. Virus, named vPader10, was rescued from this construct by electroporation of RNA transcripts into porcine PK15 cells. To further study the characteristics of vPader10, we evaluated the virulence of this virus in vivo in pigs. An animal experiment was performed where three pigs were inoculated with vPader10 and housed in-contact with two non-inoculated pigs for 5 weeks. Following inoculation with vPader10, two out of three pigs displayed severe clinical signs of CSF from PID 14 that progressed until death of the pigs at PID 21 and PID 22, respectively. High fever (,41ºC) was observed in these pigs from PID 14 and remained at a high level until day of death. ...
Classical swine fever is currently not only a problem in certain regions of Germany but also in the Eastern Cape region. An outbreak means severe economical problems. The Eastern Cape agriculture authorities even admitted that they don`t know whether they are winning the fight against swine fever.
5 September 2000 New case of swine fever in Suffolk By FWi staff A FURTHER case of classical swine fever has been confirmed on a farm in the surveillance a
The domestic swine fever virus C strain was purified by USA, Japan, local swine fever, but the domestic swine fever epidemic is still serious. Control of classical swine fever, the industry has different views, many people think is the prob
Economics of classical swine fever - CSF - hog cholera - movement ban controls on UK pig farms in protection and surveillance zones.
It is unknown how widespread the new Linda virus is in the swine population. We are currently developing a serological test to learn more about the prevalence of the Linda virus in Austria, i.e. about the number of piglets it infects, says Lamp. The test will be important because analysis of the virus has shown that it is distantly related to the virus that causes classical swine fever. We cannot exclude the possibility that it might interfere with the official tests for swine fever. In addition, it is unclear how much of a danger the newly discovered virus poses for pig breeding. The Spanish word linda is usually translated as pretty or cute but there is nothing pretty or cute about the newly discovered Linda virus. Till Rümenapf, the Head of the Institute, notes that Because it also infects the uterus of pregnant sows, the Linda virus could disrupt the development of the piglets cerebral cortex, like infection with the Zika virus. However, we should remember that the closest ...
First report focuses on African swine fever, foot-and-mouth disease and classical swine fever as the near real-time monitoring system is developed and tested.
Overall 137 programmes have been selected for EU funding to tackle animal diseases that impact on human and animal health as well as trade. The elected programmes were granted the following allocation: Bovine Tuberculosis (about €71 million); Transmissible Spongiform Encephalopathies (about €54 million); Bovine Brucellosis (about €7.4 million), rabies (€25 million); Bluetongue (€1.5 million); Classical Swine Fever (€2.5 million); Salmonellosis (about €16.6 million); Avian influenza (€2.6 million) and African Swine Fever in Sardinia (€1.4 million ...
AUSTRIA - Some newly born piglets shiver even when they are kept warm. So-called "shaking piglets" have symptoms that resemble those of the classical swine fever, with extensive damage to the brain and the spinal cord.
TY - CONF. T1 - New and emerging technologies: Improved laboratory and on-site detection of OIE List A viruses in animals and animal products. AU - Belak, Sandor. AU - Uttenthal, Åse. AU - Hakhverdyan, Mikhayil. AU - Allan, Gordon. AU - Sanchez-Vizcaino, Jose Manuel. AU - Istvan, Kiss. AU - Merza, Malik. AU - Brocchi, Emiliana. AU - van Reeth, Kristien. AU - King, Donald. PY - 2008. Y1 - 2008. KW - Classical swine fever. KW - Foot and Mouth Disease. KW - Virus. KW - OIE listed diseases. M3 - Poster. T2 - 2nd Annual Meeting EPIZONE. Y2 - 4 June 2008 through 6 June 2008. ER - ...
Alamian S, Amiry K, Bahreinipour A, Etemadi A, Tebianian M, Mehrabadi MHF and Dadar M (2021) Brucella species circulating in rural and periurban dairy cattle farms: a comparative study in an endemic area. Tropical Animal Health and Production 53:200. doi:10.1007/s11250-021-02645-y.. Amarir F, Rhalem A, Sadak A, Raes M, Oukessou M, Saadi A, Bouslikhane M, Gauci CG, Lightowlers MW, Kirschvink N and Marcotty T (2021) Control of cystic echinococcosis in the Middle Atlas, Morocco: Field evaluation of the EG95 vaccine in sheep and cesticide treatment in dogs. PLoS Neglected Tropical Diseases 15:e0009253. doi:10.1371/journal.pntd.0009253.. Bazarragchaa E, Isoda N, Kim T, Tetsuo M, Ito S, Matsuno K and Sakoda Y (2021) Efficacy of oral vaccine against classical swine fever in wild boar and estimation of the dsease dynamics in the quantitative approach. Viruses 13. doi:10.3390/v13020319.. Bertran K, Kassa A, Criado MF, Nuñez IA, Lee DH, Killmaster L, Sá E Silva M, Ross TM, Mebatsion T, Pritchard N and ...
Dr. Dilip Kumar Sarma received his B.V.Sc &A.H. Degree from Assam Agricultural University in 1978 securing first class first position. He did his M.V.Sc and Ph.D degrees in Veterinary Bacteriology and Virology from Punjab Agricultural University, Ludhiana, India in 1981 and 1987 respectively. He had one year post doctoral research training at the AFRC Institute for Animal Health, Pirbright Laboratory, Surrey, U.K. under Commonwealth Govt. Scholarship. He has worked as Lecturer, Asstt. Professor, Virologist and as a Professor in the Department of Microbiology, Assam Agricultural University, Khanapara, Guwahati, since 2004. He has received the National Fellow Award of the Indian Council of Agricultural Research, New Delhi, India to work in a project on Classical swine fever and he has been working in the project since 2005. He has guided four Ph.D students, published two textbooks and 165 research papers in the National and International Journals and several popular scientific articles. He has ...
At least 210 pigs and piglets have died due to outbreak of classical swine flu in Mizoram-Myanmar border Champhai town and Biate village in Champhai
Hog Cholera (Classical Swine Fever). Sandra Axiak and Carolin Winter. Definition of hog cholera:. highly contagious viral disease of swine can cause acute, chronic, or congenital disease considered a foreign animal disease. Foreign animal disease (FAD):. Definition: Slideshow 113381 by flora
Virions from hog cholera virus (HCV), a member of the genus Pestivirus, were analyzed by using specific antibodies. The nucleocapsid protein was found to be a 14-kDa molecule (HCV p14). An equivalent protein could also be demonstrated for virions from another pestivirus, bovine viral diarrhea virus. The HCV envelope is composed of three glycoproteins, HCV gp44/48, gp33, and gp55. All three exist in the form of disulfide-linked dimers in virus-infected cells and in virions; HCV gp44/48 and gp55 each form homodimers, whereas gp55 is also found dimerized with gp33. Such complex covalent interactions between structural glycoproteins have not been described so far for any RNA virus. ...
Background The genus pestivirus within the family Flaviviridaeincludes bovine viral diarrhoea virus (BVDV) types 1 and 2, border disease virus (BDV) and classical swine fever virus. The two...
Wang, L., S. Mi, R. Madera, L. Ganges, M.V. Borca, J. Ren; C. Cunningham, A.G. Cino-Ozuna, H. Li, C. Tu, W. Gong; and J. Shi. 2020. A neutralizing monoclonal antibody-based competitive ELISA for classical swine fever C-strain post-vaccination monitoring. BMC Veterinary Research. Open Access. https://link.springer.com/article/10.1186/s12917-020-2237-6. Borca, M.V., V. ODonnell, L.G. Holinka, G.R. Risatti, E. Ramirez-Medina, E.A. Vuono, J. Shi, S. Pruitt, A. Rai, E. Silva, L. Velazquez Salinas, and D.P. Gladue. 2020. Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine. Scientific Reports. 10:494.. Evans, T.S., Z. Shi, M. Boots, W. Liu, K. Olival, X. Xiao, S. Vandewoude, H. Brown, J. Chen, D. Civitello, L. Escobar, Y. Grohn, H. Li, K. Lips, Q. Liu, J. Lu, B. Martinez-Lopez, J. Shi, X. Shi, B. Xu, L. Yuan, G. Zhu, and W. Getz. 2020. Synergistic China-US Ecological Research is Essential for Global EID Preparedness. EcoHealth. ...
occurs in South America, Asia (including Indonesia) and certain European countries. Information on current country status can be obtained from the OIE World Animal Health Information Database. The last time CSF occurred in Australia was in 1962. Outbreaks have recently been reported in Bulgaria (in wild species), Israel and Lithuania.. Classical swine fever virus is a highly contagious Pestivirus which only infects swine, and is antigenically related to border disease virus and bovine viral diarrhoea virus. Pig to pig transmission is most common but CSF can also be spread by aerosol, swill feeding and mechanically by insects and fomites. Swill feeding of peri-urban pigs is a likely method of re-introduction of this virus to Australia and hence CSF may first be encountered by a veterinary practitioner servicing these areas. Strains vary greatly in their virulence. The last outbreak in Australia was caused by a mild strain that was detected only by a reported increase in fevered carcasses at ...
In 2016, two small-scale fattening pig farms (about 200 or 300 American Landrace pigs in total), locating in Guangdong province (southern China) and Jilin province (northern China) separated by more than 3, 000 km in distance (Fig. 1), suffered from severe respiratory disease (SRD). Pigs (2-3 months old) on both farms have received immunizations against PCV2, pseudorabies virus (PRV), classical swine fever virus (CSFV) and type 2 porcine reproductive and respiratory syndrome virus (PRRSV). However, the morbidity rate of pigs on the two farms was approximately 60%, the mortality in affected pigs was about 80%. From autopsy results of 18 individual dead pigs (n = 8 for farm A, n = 10 for farm B) (Additional file 1: Table S1), the similar macroscopic lesions were concentrated in the lungs and lymph nodes, which was characterized by PRRSV- or PCV2-like lesions including pulmonary interstitial widening, congestion and lymphadenopathy bleeding. These 18 pig lung samples were defined as the samples ...
APHIS recognizes the prevalence of CSF in Guatemala and responded immediately to their request for assistance to help control their latest outbreak, said Dr. John R. Clifford, USDAs Chief Veterinary Officer. We sent a team of subject matter experts to assess the situation and concluded that the use of much-needed vaccine would help in containing the spread of the disease.. On March 19, APHIS donated the vaccines based on a request for assistance from the Guatemalan Chief Veterinary Officer, Dr. Humberto Maldonado, to help Guatemala control the spread of CSF there.. This assistance will help control and limit spread of CSF in Guatemala and neighboring countries. Among these countries are Mexico, currently working to control and eradicate the disease, and the United States, which has been free of CSF for over 30 years. Thus, this assistance extends beyond Guatemala and contributes to safeguarding animal health throughout Central and North America.. With trade opportunities increasing each ...
swine fever virus N(pro) protein: has autoproteolytic activity; inhibits double-stranded RNA-mediated apoptosis and IFN-alpha/beta induction; GenBank AF144472
Exotic animal diseases (EADs) are characterized by their capacity to spread global distances, causing impacts on animal health and welfare with significant economic consequences. We offer a critique of current import risk analysis approaches employed in the EAD field, focusing on their capacity to assess complex systems at a policy level. To address the shortcomings identified, we propose a novel method providing a systematic analysis of the likelihood of a disease incursion, developed by reference to the multibarrier system employed for the United Kingdom. We apply the network model to a policy-level risk assessment of classical swine fever (CSF), a notifiable animal disease caused by the CSF virus. In doing so, we document and discuss a sequence of analyses that describe system vulnerabilities and reveal the critical control points (CCPs) for intervention, reducing the likelihood of U.K. pig herds being exposed to the CSF virus. ...
How BVDV affects fetal development depends on the timing of maternal infection during pregnancy. The eyes and central nervous system are targets of the virus, and characteristic deformations of the brain and skull are observed. These deformities include hydranencephaly, hydrocephalus, and microencephaly [6].. BVDV is not transmissible to people but can infect a number of ruminants. Other species in the pestivirus family - siblings to bovine viral diarrhea - include border disease of sheep and classical swine fever in pigs. All of these pestiviruses can cause birth deformities.. Another fascinating aspect of pestiviruses is that they can affect host immunity during infection. BVDV is capable of becoming a persistent infection that is never fully cleared from the body. Persistent infection can happen in a number of ways. BVDV can manipulate the mechanisms that identify pathogens in the body. The virus is therefore camouflaged as self and avoids targeting by the immune system [7]. Calves may be ...
Imports of pork rinds, a snack food made from deep-fried pork skins, are so well cooked that the risk of transmitting hog diseases, such as foot-and-mouth disease, swine vesicular disease, African swine fever and classical swine fever, is non-existent, said the U.S. Department of Agriculture's Animal and Plant Health Inspection Service.
The European Commission said that given the serious impact that animal disease outbreaks can have on human health, society, the economy and trade, the allocation of EU co-financing will continue to assist national authorities to put in place precautionary measures, disease surveillance and eradication programmes, at national and European level.. Overall 142 programmes have been selected for EU funding: Bovine Tuberculosis (about €56 million); Transmissible Spongiform Encephalopathies (about €42 million); Rabies (€27.5 million); Salmonellosis (about €17.5 million); Bovine Brucellosis (about €10 million); Classical Swine Fever (€3 million); Avian Influenza (€2.5 million); Bluetongue (€1.5 million); African Swine Fever and Swine Vesicular Disease in Italy (about €850.000).. EU co-financing has resulted in a continuous decrease in the number of cases of diseases, such as Salmonellosis (the second most frequently reported zoonotic disease in humans) and Transmissible Spongiform ...
Swine leukocyte antigen (SLA) class I molecules play a crucial role in generating specific cellular immune responses against viruses and other intracellular pathogens. They mainly bind and present antigens of intracellular origin to circulating MHC I-restricted cytotoxic T lymphocytes (CTLs). Binding of an appropriate epitope to an SLA class I molecule is the single most selective event in antigen presentation and the first step in the killing of infected cells by CD8+ CTLs. Moreover, the antigen epitopes are strictly restricted to specific SLA molecules. In this study, we constructed SLA class I complexes in vitro comprising viral epitope peptides, the extracellular region of the SLA-1 molecules, and β2-microglobulin (β2m) using splicing overlap extension polymerase chain reaction (SOE-PCR). The protein complexes were induced and expressed in an Escherichia coli prokaryotic expression system and subsequently purified and refolded. Specific binding of seven SLA-1 proteins to one classical swine fever
Romania will be resuming on-farm vaccinations against classical swine fever (CSF) soon, the website of the US Department of Agriculture says.
At the IVMR, a rich past and an intense present complement each other. József Csontos (1889-1962), founding director of the then-called Animal Health Research Institute, together with Aladár Aujeszky developed the so-called carbolic glycerinated vaccine against rabies containing fixed virus. After World War II, he played a pivotal role in the eradication of infectious animal diseases, above all classical swine fever and porcine erysipelas, and also took part in organizing the healthcare of large stocks in intensive animal farming. Among past researchers of the institute, one of worldwide fame was Domokos Derzsy (1914-1975), whose scientific topics of interest spanned almost the entire spectrum of infectious animal diseases. He diagnosed and described several poultry diseases unknown in Hungary before, one of these was that caused by goose Parvovirus, which was named Derzsys disease in honor of his internationally significant research on this pathogen. Adorján Bartha (1923-1996) also worked ...
At the IVMR, a rich past and an intense present complement each other. József Csontos (1889-1962), founding director of the then-called Animal Health Research Institute, together with Aladár Aujeszky developed the so-called carbolic glycerinated vaccine against rabies containing fixed virus. After World War II, he played a pivotal role in the eradication of infectious animal diseases, above all classical swine fever and porcine erysipelas, and also took part in organizing the healthcare of large stocks in intensive animal farming. Among past researchers of the institute, one of worldwide fame was Domokos Derzsy (1914-1975), whose scientific topics of interest spanned almost the entire spectrum of infectious animal diseases. He diagnosed and described several poultry diseases unknown in Hungary before, one of these was that caused by goose Parvovirus, which was named Derzsys disease in honor of his internationally significant research on this pathogen. Adorján Bartha (1923-1996) also worked ...
Outbreaks of notifiable infectious animal diseases, such as foot-and-mouth disease, classical swine fever and avian influenza, have major societal and economic consequences. Fortunately, such animal diseases occur infrequently in the Netherlands. Wageningen Bioveterinary Research (WBVR) contributes to this status by designing risk-based surveillance systems.
26 September 2000 Unigate blames losses on swine fever UNIGATE has blamed the outbreak of swine fever for preventing its pork processing plant in North Yor
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Countries across Asia are struggling to contain an outbreak of swine fever, which is threatening the livelihoods of millions of families that rely on pig farming. The highly contagious and incurable virus is deadly to pigs but not dangerous to humans. The disease originally spread from Africa
Another case of swine fever was detected in Manta in Cahul district. The virus was confirmed by laboratory analysis after a man announced to the National Agency for Food Safety in the district that he had found his pig dead.Immediately, the emergency comm
Global price cycle, Swine Fever outbreak in S Korea, China bans Dutch food imports, USDA forecasts, EU vets vote for new Spanish and German CSF measures, UK farmers suffer second FMD scare.....
Sigma-Aldrich offers abstracts and full-text articles by [Jiangnan Li, Yajuan Dai, Shuai Liu, Huancheng Guo, Tiedong Wang, Hongsheng Ouyang, Changchun Tu].
In der medizinischen Versorgung bekommt die Ernährung einen immer größeren Stellenwert. Zum einen sind heute Nahrungsmittelunverträglichkeiten häufiger anzutreffen, andererseits wird eine Ernährungsth
Klassisches Schweinepest-Virus - en. Classical swine fever virus (CSFV) oder Hog cholera virus ... Usutu-Virus - en. Usutu virus (USUV), Zika-Virus - en. Zika virus (ZIKV), sowie Gelbfieber-Virus - en. Yellow fever virus (YFV) ... Genus ‚Negevirus', mit Species ‚Blackford virus', ‚Bofa virus', ‚Buckhurst virus', ‚Marsac virus', sowie ‚Muthill virus'[53] ... Sorghum mosaic virus (SrMV), Lily-Mottle-Virus - en. Lily mottle virus (LMoV), sowie Sellerie-Virus Y - en. Apium virus Y (ApVY ...
... classical swine fever,[109] avian influenza,[110] Actinobacillus pleuropneumonia[111] and Salmonella infections in pigs.[112] ... The DIVA strategy has been applied in various countries and successfully eradicated pseudorabies virus. Swine populations were ... "Glycoprotein E1 of hog cholera virus expressed in insect cells protects swine from hog cholera". Journal of Virology. 67 (9): ... Examples include the subunit vaccine against Hepatitis B virus that is composed of only the surface proteins of the virus ( ...
... classical swine fever, African swine fever and contagious bovine pleuropneumonia. Approximately 10% of the facility will be ... "Dangerous Animal Virus on US Mainland?". The Associated Press. 2008-04-11. Retrieved 2010-07-21. No NBAF in Kansas "Officials ...
Indian swine flu (2015). *MERS in South Korea (2015). *Angolan yellow fever (2016) ... Post-classical. *First plague pandemic (541-767). *Plague of Justinian (541-542) ... Kerala Nipah virus (2018). *Équateur province Ebola (2018). *Kivu Ebola (2018-present) ...
Indian swine flu (2015). *South Korean MERS (2015). *Angolan yellow fever (2016) ... "Origin of measles virus: divergence from rinderpest virus between the 11th and 12th centuries.", Virol. J., 7 (52). ... Post-classical. *First plague pandemic (541-767). *Plague of Justinian (541-542) ... He described the plague as "great" and of long duration, and mentioned fever, diarrhea, and pharyngitis as well as a skin ...
Indian swine flu (2015). *South Korean MERS (2015). *Angolan yellow fever (2016) ... Post-classical. *First plague pandemic (541-767). *Plague of Justinian (541-542) ... Kerala Nipah virus (2018). *Équateur province Ebola (2018). *Kivu Ebola (2018-2020) ...
Indian swine flu (2015). *South Korean MERS (2015). *Zika (2015-2016). *Angolan yellow fever (2016) ... Post-classical. *First plague pandemic (541-767). *Plague of Justinian (541-542) ... Kerala Nipah virus (2018). *Équateur province Ebola (2018). *Kivu Ebola (2018-2020) ...
... and swine influenza viruses, allowing for mixtures of genes (reassortment) to create a new virus, which can cause an antigenic ... Post-classical. *First plague pandemic (541-767). *Plague of Justinian (541-542) ... Angolan yellow fever (2016). *Yemeni cholera (2016-present). *Gorakhpur Japanese encephalitis (2017) ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ...
This ancestor virus diverged about 1913-1915 into two clades (or biological groups), which gave rise to the classical swine and ... yellow fever, diphtheria, and cholera all occurred near the same time. These outbreaks probably lessened the significance of ... One hypothesis is that the virus strain originated at Fort Riley, Kansas, in viruses in poultry and swine which the fort bred ... Both the H2N2 and H3N2 pandemic strains contained avian flu virus RNA segments. "While the pandemic human influenza viruses of ...
If due to a virus, the condition usually resolves within one week.[18] Some viral infections also involve fever, fatigue, ... Zimmerman, Jeffrey; Karriker, Locke; Ramirez, Alejandro; Kent Schwartz; Gregory Stevenson (15 May 2012). Diseases of Swine ( ... Classical diabetic ketoacidosis (DKA) presents with abdominal pain, nausea, and vomiting, but without diarrhea.[1] One study ... DNA virus. HBV (B). RNA virus. CBV. HAV (A). HCV (C). HDV (D). HEV (E). HGV (G). ...
Indian swine flu (2015). *South Korean MERS (2015). *Angolan yellow fever (2016) ... Post-classical. *First plague pandemic (541-767). *Plague of Justinian (541-542) ... Kerala Nipah virus (2018). *Équateur province Ebola (2018). *Kivu Ebola (2018-2020) ...
"Pathway analysis in blood cells of pigs infected with classical swine fever virus: comparison of pigs that develop a chronic ... Dynamics of virus excretion via different routes in pigs experimentally infected with classical swine fever virus strains of ... The Npro product of classical swine fever virus and bovine viral diarrhea virus uses a conserved mechanism to target interferon ... Mutations abrogating the RNase activity in glycoprotein E(rns) of the pestivirus classical swine fever virus lead to virus ...
Classical swine fever virus induces oxidative stress in swine umbilical vein endothelial cells. Overview of attention for ... Classical swine fever virus induces oxidative stress in swine umbilical vein endothelial cells ...
Challenge of Pigs with Classical Swine Fever Viruses after C-Strain Vaccination Reveals Remarkably Rapid Protection and ... Challenge of Pigs with Classical Swine Fever Viruses after C-Strain Vaccination Reveals Remarkably Rapid Protection and ...
Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen ... Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen ... Development and evaluation of a rapid immunomagnetic bead assay for the detection of classical swine fever virus antigen ... Drug-resistant enteric fever, antibiotics and the need for clean water * Five years at the top: Oxford retains first place in ...
Zdravilo Porcilis Pesti vsebuje virus klasične prašičje kuge - antigenska podenota E2.. Za kaj se zdravilo Porcilis Pesti ... Porcilis Pesti (Classical Swine Fever Virus (CSFV) -E2...) - QI09AD04. *Porcilis Pesti. *Povzetek glavnih značilnosti zdravila ... Classical Swine Fever Virus (CSFV) -E2 subunit antigen. Proizvajalec:. Intervet International BV. ...
The swine vascular endothelial cell is a primary target cell for CSFV. The aim of this study was to determine the role of CSFV ... We demonstrated that CSFV infection induced oxidative stress in swine umbilical vein endothelial cells (SUVECs), characterized ... Classical swine fever virus (CSFV) infection causes significant losses of pigs, which is characterized by hemorrhage, ... From: Classical swine fever virus induces oxidative stress in swine umbilical vein endothelial cells ...
Transmission of classical swine fever virus within herds during the 1997-1998 epidemic in The Netherlands. Prev. Vet. Med. 42, ... 1999 Efficacy and stability of a subunit vaccine based on glycoprotein E2 of classical swine fever virus. Vet. Microbiol. 66, ... 2000 Determination of the onset of the herd-immunity induced by the E2 sub-unit vaccine against classical swine fever virus. ... 2004 Efficacy of E2-sub-unit marker and C-strain vaccines in reducing horizontal transmission of classical swine fever virus in ...
The same integration site could also be used to label the related Classical swine fever virus. Also, BVDVE2_fluo particles ... The fluorophore E2 fusion protein was readily detectable in purified virus particles by Western blot and fluorescence ... BVDV-1 virus (BVDVE2_fluo). This insertion was well tolerated, resulting in a 2-fold drop in titer compared to the parental ... Genetic labelling of viruses with a fluorophore allows to study their life cycle in real time, without the need for fixation or ...
4. Detection of classical swine fever virus (CSFV) E2 and Erns antibody (IgG, IgA) in oral fluid specimens from inoculated (ALD ... Classical swine fever virus; antibodies; blood serum; cost effectiveness; cross reaction; enzyme-linked immunosorbent assay; ... responses against classical swine fever virus (CSFV) E2 and Erns proteins in pigs (n = 60) inoculated with a moderately ... Torque teno sus virus (TTSuV) infection is common worldwide in both healthy and diseased swine and a relationship between this ...
African swine fever virus (ASFV). Launch JBrowse Classical swine fever virus (CSFV). ... This resource has been developed for U.S. and international swine disease researchers and has been funded by the USDA-ARS (# ...
Jeff Zimmerman noted that detection of African Swine Fever Virus (ASFV), Classical Swine Fever Virus (CSFV), or Foot-and-Mouth ... The University of Minnesota Swine Health Monitoring Project showed that with the entry of Porcine Epidemic Diarrhea Virus (PEDV ... J Swine Health Prod 21: 72-84.. 6Zimmerman, J. 2017. Swine medicine in the 21st century: Immovable object meets unstoppable ... 4 It has been estimated that Porcine Respiratory and Reproduction Syndrome Virus (PRRSV) results in annual losses to the swine ...
Classical swine fever virus, African swine fever virus, Rift Valley virus, prions, highly pathogenic influenza viruses, ... West Nile virus, tuberculosis, avian Influenza, entomological surveys for West Nile… ...
Classical swine fever virus, African swine fever virus, Rift Valley virus, prions, highly pathogenic influenza viruses, ... West Nile virus, tuberculosis, avian Influenza, entomological surveys for West Nile… ...
Classical swine fever virus (1). *Clostridium (1). More results… Organisms. *birds (1) ... Agglutination of the Trypanosomes of Sleeping Sickness, Nagana, Dourine, and Congo Coast Fever, and Consideration of the ...
Classical swine fever virus (3). *Clostridium perfringens (3). *dogs (3). *equine infectious anemia virus (3) ... Adult cattle were also affected and had fever followed by anaemia and jaundice. In some cows there was permanent udder damage ... and for swine compensation and border crossings from Queensland (cattle tick control). Diseases... ...
... and suggests that multiple coexisting subpopulations may allow the virus to adapt to its human host populations. ... Hepatitis C virus (HCV) has been the subject of intense research and clinical investigation as its major role in human disease ... ribosome binging to the initiation codon during internal translation initiation of hepatitis C and classical swine fever virus ... Cristina J: Genetic diversity and evolution of hepatitis C virus in the Latin American region. J Clin Virol 2005, 34: S1-S7. ...
... containing attenuated C strain Classical Swine Fever virus. Vaccine has high stability in safety and effectiveness. INDICATION ... containing attenuated C strain Classical Swine Fever virus. It is can be used as normal Hog… ... SWINE SALMONELLA VACCINE TECHNICAL FEATURES · Inactivated vaccine containing killed Salmonella cholerae suis strains S1, S2, S3 ... BIVALENT VACCINE SWINE PASTEURELLA AND ERYSIPELAS (Attenuated freeze-dried vaccine) TECHNICAL FEATURES · Freeze-dried vaccine, ...
Swine diseases. *African swine fever. · Classical swine fever. · Nipah virus encephalitis · Porcine cysticercosis. · Porcine ... Q fever · Rabies. · Rift Valley fever. · Rinderpest. · Surra (Trypanosoma evansi) · Trichinellosis. · Tularemia. · Vesicular ... Duck virus hepatitis · Fowl cholera. · Fowl typhoid. · Highly pathogenic avian influenza and low pathogenic avian influenza in ... Crimean Congo haemorrhagic fever · Echinococcosis/hydatidosis. · Epizootic haemorrhagic disease. · Equine encephalomyelitis ( ...
The third incident involved a release of Classical Swine Fever virus, also known as hog cholera, which resulted in the illness ... Many viruses that are closely related to H.I.V., such as the Visna virus, which is a fatal immune-deficiency virus of sheep, do ... The emergence was subtle: the virus incubates for years in a human host before it kills the host.. A zoonotic virus is a virus ... Yellow fever, which is considered a lethal virus, kills only five per cent of the infected once they reach a hospital.. Marburg ...
African Swine Fever, Classical Swine Fever (Hog Cholera), Swine Influenza Virus and Foot & Mouth Disease which can affect ...
Immunopathogenesis of persistent and subclinical infections generated by classical swine fever virus ... home: 26/06/2017 Fi: 25/06/2020 New virus causing diarrhea in Spain ...
Since it first arrived in Asia in 2018, African swine fever virus has caused a devastating pandemic resulting in more than a ... research in eastern Indonesia on the evaluation of the risk for highly pathogenic avian influenza and classical swine fever ... Combating African Swine Fever in Timor-Leste with Associate Professor Paul Hick ... Associate Professor Paul Hick speaks to Dr Thushara Dibley about his work reducing the impact of African swine fever and other ...
CSFV (Classical Swine Fever Virus) CSFV MoreContact. * PED (Porcine Epidemic Diarrhea Virus) PED MoreContact ... ASFV (African Swine Fever Virus) ASFV MoreContact. * PRRSV (Porcine Respiratory & Reproductive Syndrome Virus) PRRSV More ...
He was a disease expert for UK lab responses to porcine reproductive and respiratory syndrome (1990), classical swine fever ( ... In 2001, he completed a PhD on the subject Equine herpesvirus 1 and 4 and equine influenza virus infections: diagnosis, ... such as African swine fever, is at an all-time high.. *Do you know what your role will be if the U.S. is hit with an outbreak ... and to better trace the methods and pathways of virus spread. He has more than 200 publications in refereed journals. He has ...
The N-terminal domain of N-pro of classical swine fever virus determines its stability and regulates type I IFN production. -. ...
Animal diseases of viral ethiology: Foot and Mouth Disease Virus, Classical Swine Fever Virus, African Swine Fever Virus, ... Emerging zoonotic diseases: Rift Valley Fever Virus , West Nile Fever Virus and Influenza virus ... Recent advances in the development of recombinant vaccines against classical swine fever virus: Cellular responses also play a ... African swine fever virus pB119L protein is a flavin adenine dinucleotide-linked sulfhydryl oxidase. Rodríguez I, Redrejo- ...
There has been a significant decline in pigs with Classical Swine Fever at the end of the year. However, the ban on the import ... The virus was first reported in February 2007.. For the latest news via email, click here…. ... A decrease in the number of domestic birds infected by the H5N1 virus (bird flu) in the Moscow area has been recorded. ...
Additionally, the country has not had any outbreaks of African Swine Fever (ASF), Classical Swine Fever (CSF), Porcine Epidemic ... In addition, just Seneca virus as well as influenza have been detected on Brazilian swine farms but these are not reportable to ... Quite a few market experts foresee rising prices for pigs outside Asia now African Swine Fever has been creating... ... ESPHM review, day 2: Focus on swine nutrition On Apr 16. In Nutrition ...
Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM ... In addition to the classical microscopy ... Confocal Microscopy of Reovirus Transport in Living Dorsal Root Ganglion Neurons ... Detection and Quantification of African Swine Fever Virus in MA-104 Cells Authors: Ayushi Rai, Sarah Pruitt, Elizabeth Ramirez- ... such as yellow fever virus (YFV), Zika virus (ZIKV), and dengue virus (DENV), among others. Presently, the identification of ...
... and classical swine fever (CSF) since March this year, officials ... Centre directs Mizoram to cull virus-affected pigs. The Centre ...
The virus spread rapidly afterwards and has been devastating the global swine industry since the early 1990s. Among individual ... Its similarity to so-called classical PRRS viruses was only in the range 70-86%. Moreover, the sequencing of its genome ... Chinese scientists reported to the 2007 international PRRS symposium that the disease has been marked by a prolonged high fever ... Each of 42 viruses isolated belonged to the North American type. From follow-up analyses it emerged that Taiwans version of ...
Swine fever (classical) *Swine fever (African) *Swine influenza *Swine influenza (in pigs) FAQs ... Surveillance program for white spot syndrome virus *Surveillance program for RBE and NLIS ...
Capripox viruses (1) *Chlamydiosis (2) *Chronic wasting disease (CWD) (1) *Classical swine fever (Hog cholera) (1) ... Tick-borne, Japanese encephalitis; West Nile, Dengue, Yellow, Zika fever; Usutu virus (1) ...
Capripox viruses (1) *Chlamydiosis (2) *Chronic wasting disease (CWD) (1) *Classical swine fever (Hog cholera) (1) ... Tick-borne, Japanese encephalitis; West Nile, Dengue, Yellow, Zika fever; Usutu virus (1) ... Animal species: Sheep, Deer, Swine, Bovine, Cat, Goat, Fur-bearing animal, Laboratory animal, Dog, Horse ...
Classical Swine Fever. Contact. Friedrich-Loeffler-Institut. Südufer 10. 17493 Greifswald - Insel Riems. Telephone: +49 (0) ... The etiologic agent is a double-stranded RNA virus (African Horse Sickness Virus, AHSV) in the Orbivirus genus (family ... Laboratory for phylogenetic analyses with emphasis on influenza viruses and borna viruses ... Laboratory for phylogenetic analyses with emphasis on influenza viruses and borna viruses ...
... companion diagnostic checks are in the market and could also be utilized for foot-in-mouth illness and classical swine fever . ... Technologies for Improving Animal Health and Production 191 BOX 6-7 RNAi Technology to Resist Bluetongue Virus Bluetongue is a ... The capability to tell apart between animals uncovered to a complete virus and vaccinated animals constantly and reliably could ...
Classical Swine Fever. Contact. Friedrich-Loeffler-Institut. Südufer 10. 17493 Greifswald - Insel Riems. Telephone: +49 (0) ... Laboratory for phylogenetic analyses with emphasis on influenza viruses and borna viruses ... Laboratory for phylogenetic analyses with emphasis on influenza viruses and borna viruses ... Laboratory for Immunology and Molecular Biology of positive strand RNA Viruses * Laboratory for Mucosal Immunology and ...
ASF (African Swine Fever) (4). *Aujeszkys Disease (2). *Brucellosis (Swine) (2). *Classical Swine Fever ... Real-time RT-PCR for the qualitative detection of all serotypes of the BVD / BD viruses in ruminant blood, serum, milk, organs ...
Swine vesicular disease (1) *Tick-borne, Japanese encephalitis; West Nile, Dengue, Yellow, Zika fever; Usutu virus (1) ... Classical swine fever (Hog cholera) (7) *Clinical biochemistry (25) *Colibacteriosis (2) *Contagious equine metritis (2) ... Animal species: Sheep, Deer, Swine, Bovine, Cat, Goat, Fur-bearing animal, Laboratory animal, Bird, Dog, Horse ...
  • Porcilis Pesti (Classical Swine Fever Virus (CSFV) -E2. (healthsonar.com)
  • The objective of this study was to describe oral fluid and serum antibody (IgG, IgA) responses against classical swine fever virus (CSFV) E2 and Erns proteins in pigs (n = 60) inoculated with a moderately virulent field strain (ALD, n = 30) or a modified live virus vaccine strain (LOM, n = 30). (usda.gov)
  • Pathway analysis in blood cells of pigs infected with classical swine fever virus: comparison of. (deepdyve.com)
  • Once pigs are infected, the virus remains in their bodies, so infected pigs can continue to release the virus for a long time and are extremely contagious to other pigs. (vetmeduni.ac.at)
  • The fellowship projects, jointly funded by the IVVN and Canada's International Development Research Centre, will focus on a variety of pathogens including coronaviruses, tilapia lake virus, Newcastle disease virus, Salmonella enterica , Streptococcus agalactiae , Staphylococcus aureus and Theileria annulata . (campaign-archive.com)
  • Within the Flaviviridae , infection dynamics have been analysed for DiD labelled Dengue virus, revealing diffusion patterns on the cell surface and the recruitment dynamics of endosomal components during virus entry 8 . (nature.com)
  • Till Rümenapf, the Head of the Institute, notes that "Because it also infects the uterus of pregnant sows, the Linda virus could disrupt the development of the piglets' cerebral cortex, like infection with the Zika virus. (vetmeduni.ac.at)
  • Infection of the uterus with the Bungowannah virus leads to abortion and a high mortality among piglets, generally caused by a viral infection of the heart muscle. (vetmeduni.ac.at)
  • Bovine viral diarrhoea virus (BVDV) is an economically important pathogen within the family Flaviviridae , genus Pestivirus and has been extensively used as a model for hepatitis C virus research. (nature.com)
  • Yet, the attachment and entry of BVDV or any other pestivirus has never been analysed in real time as suitable labelled viruses have been lacking to date. (nature.com)
  • Infectious progeny virus was released in the supernatant, with an average titer of 6.8 × 10 5 ffu/ml 48 h after transfection (n = 4). (nature.com)
  • We are currently developing a serological test to learn more about the prevalence of the Linda virus in Austria, i.e. about the number of piglets it infects," says Lamp. (vetmeduni.ac.at)
  • So-called "shaking piglets" have symptoms that resemble those of the classical swine fever, with extensive damage to the brain and the spinal cord. (vetmeduni.ac.at)
  • The new PCR assay our team developed recognized all known pestiviruses," explains Benjamin Lamp of the Institute of Virology," and so enabled us to detect a virus that was previously unknown. (vetmeduni.ac.at)
  • Zdravilo Porcilis Pesti vsebuje virus klasične prašičje kuge - antigenska podenota E2. (healthsonar.com)
  • The fluorophore E2 fusion protein was readily detectable in purified virus particles by Western blot and fluorescence microscopy and the particle integrity and morphology was confirmed by cryo electron microscopy. (nature.com)
  • To gain dynamic information of virus entry, viruses can be genetically labelled by the fusion of a structural protein with a fluorophore or by labelling components of intact virus particles with a fluorescent dye (e.g. adenovirus/enveloped particles with DiD) (reviewed in 7 ). (nature.com)
  • Classical swine fever (CSF) or hog cholera is a highly contagious swine viral disease. (physiciansweekly.com)
  • This means that it is safe to work with highly contagious animal diseases such as foot-and-mouth disease and classical swine fever. (wur.nl)
  • MANHATTAN - A Kansas State University researcher has licensed a new vaccine to an animal health company to fight a highly contagious swine disease overseas. (k-state.edu)
  • Included among the diseases to be studied at the research lab are: foot-and-mouth disease, classical swine fever, African swine fever and contagious bovine pleuropneumonia. (wikipedia.org)
  • Classical swine fever virus replicon particles lacking the E rns gene: a potential marker vaccine for intradermal application. (microbiologyresearch.org)
  • Nevertheless, animals inoculated with an E2 subunit vaccine are unable to prevent horizontal and vertical spread of the virus [11]. (03964.com)
  • Molecular epidemiology studies in these regions suggests that the virus circulating in the field has evolved under the positive selection pressure exerted by the immune response to the vaccine, leading to new attenuated viral variants. (biomedcentral.com)
  • The purpose of this study is to determine whether immune globulin can limit the amount of yellow fever vaccine virus present in the blood after vaccination without compromising the immunit. (bioportfolio.com)
  • Jishu Shi, professor of vaccine immunology and director of U.S.-China Center for Animal Health in the university's College of Veterinary Medicine, has developed a method of producing a classical swine fever vaccine safely and inexpensively. (k-state.edu)
  • Shi's vaccine uses a protein from the virus rather than live or attenuated virus, which means the vaccine poses no biosecurity risk to produce in the U.S., where classical swine fever was eradicated in 1978. (k-state.edu)
  • In contrast to the classical (non-marker) C-strain vaccine, this marker vaccine has the advantage that the accompanying serological test can discriminate between infected and vaccinated animals. (royalsocietypublishing.org)
  • How close are we to developing a vaccine for African swine fever? (thepigsite.com)
  • We speak to the head of the African swine fever group at The Pirbright Institute about the hard work going into developing a vaccine that could protect the global pig herd from a deadly foe. (thepigsite.com)
  • Early in June 2019, reports emerged from China that shopping mall operator Guangdong Highsun Group Co had announced it was backing the development of an African swine fever vaccine. (thepigsite.com)
  • Many experts were also sceptical of the research behind the development of the vaccine, and rightly so, as China's Ministry of Agriculture later released a statement that the company had not received a research application and that without experimental data, the claim that a polysaccharide injection is effective in preventing the virus lacked scientific proof. (thepigsite.com)
  • The Pirbright Institute have their very own African swine fever group who are certainly making progress in their ASF vaccine research. (thepigsite.com)
  • We have been conducting research to underpin vaccine development by improving understanding of how the virus evades hosts defences, especially the main antiviral response type I Interferon," explains Dr Dixon. (thepigsite.com)
  • Our second approach for vaccine development involves identifying which of the viruses 170 genes code for proteins that are immunogenic and can induce a cellular or antibody response. (thepigsite.com)
  • Improved vaccination strategies are urgently needed to curb the global spread of this virus, and the development of suitable vaccine candidates will be aided by an improved understanding of IBV molecular biology. (asm.org)
  • Recent advances in the development of recombinant vaccines against classical swine fever virus: cellular responses also play a role in protection. (nih.gov)
  • CSF endemic countries have to use routine vaccination with modified live virus (MLV) vaccines to prevent and control CSF. (physiciansweekly.com)
  • The potency of the E2 subunit vaccines is based mainly on induction of an effective humoral immune response, measurable as virus-neutralising antibodies [4, 5, 15, 37, 38]. (03964.com)
  • To date, many kinds of classical swine fever (CSF) vaccines have been developed to protect against this disease . (bvsalud.org)
  • The use of antibodies in viral research is essential in the continuing efforts to develop vaccines and drug therapies for viruses. (antibodies-online.com)
  • This knowledge has been applied to the development of gene-deleted live attenuated African swine fever virus vaccines . (thepigsite.com)
  • Researchers of the Department of Biosciences and Bioengineering had earlier developed recombinant vaccines against Japanese encephalitis and classical swine fever virus. (thehindu.com)
  • Recent research has indicated that the human MxA protein inhibits the life cycles of certain RNA viruses, such as members of the Bunyaviridae family, the Flaviviridae family and others. (beds.ac.uk)
  • Pestiviruses are enveloped viruses within the family Flaviviridae that have a highly variable single-stranded positive-sense RNA genome of ≈12.3 kb ( 1 ). (cdc.gov)
  • Bovine viral diarrhoea virus (BVDV) is an economically important pathogen within the family Flaviviridae , genus Pestivirus and has been extensively used as a model for hepatitis C virus research. (nature.com)
  • The viruses that comprise the Flaviviridae family are small enveloped, single-stranded, positive-sense RNA viruses. (asmscience.org)
  • Examination of interferon (IFN) signaling in infected cells has revealed strategies by which Flaviviridae members attenuate IFN-a/β receptor signaling actions, thus limiting the downstream expression of ISGs that otherwise control virus replication and spread. (asmscience.org)
  • In vitro effect of classical swine fever virus on a porcine aortic endothelial cell line. (microbiologyresearch.org)
  • However, an approach using expression of recombinant porcine IFN, which inhibited foot and mouth disease in swine, was less successful when applied against CSF. (defra.gov.uk)
  • Porcine epidemic diarrhea virus (PEDV) is a highly effective pathogen that can cause death of new-born piglet, resulting in big economical loss in pig farming industry. (springer.com)
  • The specificity analysis results showed that this new ICA had no cross reaction in the presence of other porcine viruses. (springer.com)
  • The genome of Rabies virus, Borna disease virus and Ebola virus code for the P phosphoprotein and VP35 that can block the antiviral response induced by IFN [ 8 , 9 , 10 ]. (intechopen.com)
  • Introductions of these viruses from humans and swine into southeast Mexico are indicated as groups of 8 horizontal lines, and each line represents 1 of 8 segments of the virus genome. (cdc.gov)
  • This insertion was well tolerated, resulting in a 2-fold drop in titer compared to the parental virus, and remained stably integrated into the genome for more than 10 passages. (nature.com)
  • These include reports describing virus morphology, the function and antigenic analysis of virus structural components, virus genome structure and expression, analysis on virus replication processes, virus evolution in connection with antiviral interventions, effects of viruses on their host cells, particularly on the immune system, and the pathogenesis of virus infections, including oncogene activation and transduction. (antibodies-online.com)
  • Pestiviruses are enveloped, plus-stranded RNA viruses whose genome comprises one long open reading frame ( 4 , 14 , 15 ). (asm.org)
  • The genome of positive-strand RNA viruses functions as mRNA from which all viral proteins necessary for virus propagation are translated. (pnas.org)
  • For the viruses listed below, regulation is limited to positive strand RNA forms of the viral genome which can be translated into protein precursors for virus production. (cdc.gov)
  • More than 30 different subytypes of Influenza A Virus are available, like anti-Influenza A Virus (H5N1) . (antibodies-online.com)
  • We conducted a 3-year longitudinal serologic survey on an open cohort of poultry workers, swine workers, and general population controls to assess avian influenza A virus (AIV) seroprevalence and sero. (bioportfolio.com)
  • Thiel HJ, Stark R, Weiland E, Rumenapf T, Meyers G (1991) Hog cholera virus: molecular composition of virions from a pestivirus. (springer.com)
  • An atypical pestivirus of swine emerged in 2003 in a commercial pig-breeding farm in Australia and was later termed Bungowannah virus (BV). (cdc.gov)
  • Congenital tremor visible in piglets that were affected by a novel pestivirus provisionally termed Linda virus, Austria, 2015. (cdc.gov)
  • Yet, the attachment and entry of BVDV or any other pestivirus has never been analysed in real time as suitable labelled viruses have been lacking to date. (nature.com)
  • Taking advantage of a previously described site within the structural protein E2, a fluorophore was introduced into a cytopathogenic (cpe) BVDV-1 virus (BVDV E2_fluo ). (nature.com)
  • Home Illinois Swine Technical Articles Effect on growth performance and bone mineralization of GraINzyme phytase in diets. (swineweb.com)
  • The second objective of this project is to establish the role that this and other viral proteins have in the pathogenesis and virulence of the virus by identifying host proteins that the viral proteins interact with. (defra.gov.uk)
  • Most viruses have multiple mechanisms that antagonise the innate immune system and this objective also aims to investigate the involvement of another viral protein in pathogenesis and virulence. (defra.gov.uk)
  • All the identified virus isolates were classified as ASFV genotype I based on the partial B646L gene (C-terminal end of VP72 gene) and the full E183L gene encoding p54 protein analysis. (bioportfolio.com)
  • If the amino acid motif is similar to that observed for other HPAI isolates, then the isolate being tested should be considered HPAI and the virus is controlled for export. (ufl.edu)
  • Nyalugwe EP, Jones RAC, Barbetti MJ et al (2015) Biological and molecular variation amongst Australian turnip mosaic virus isolates. (springermedizin.de)
  • Although chronic infections are rare events, their epidemiological impact is very high due to the long-term shedding of virus. (deepdyve.com)
  • Neighbourhood infections of classical swine fever during the 1997-1998 epidemic in The Netherlands. (semanticscholar.org)
  • One area of importance in this respect is the induction of virus-specific cytotoxic T lymphocytes (CTL), an important defence mechanism against non-cytopathogenic virus infections such as CSF. (03964.com)
  • Trends in Emerging Viral Infections of Swine includes sections on global trade, vaccination regimens against new and emerging viruses, epidemiology and control, as well as significant new outbreaks like the West Nile virus. (wiley.com)
  • This knowledge will be invaluable for the development of intervention strategies that seek to manipulate the host's innate immune response to prevent the virus from initiating new infections. (defra.gov.uk)
  • Clinical and Serological Evaluation of LINDA Virus Infections in Post-Weaning Piglets. (nih.gov)
  • In Brasier A, García-Sastre A, Lemon S (ed), Cellular Signaling and Innate Immune Responses to RNA Virus Infections . (asmscience.org)
  • To further identify the contributions of the glycoproteins to rabbit adaptation, a series of C-strain-based chimeric viruses containing single or double glycoprotein substitutions of the Shimen strain were generated and inoculated into rabbits. (springer.com)
  • This effect could be neutralized by rescue of the inactivated E rns gene and by neutralizing polyclonal antibodies directed against E rns , indicating that this effect was an inherent property of the RNase-negative virus. (asm.org)
  • Zheng GH, Peng DW, Tong QX et al (2017) Occurrence of turnip mosaic virus in Phalaenopsis sp. (springermedizin.de)
  • Profiles of rectal temperature, viral RNA, E2 protein expression, and antibody responses were compared among the chimeric viruses. (springer.com)
  • However, substitution of both E rns and E2 in the C-strain backbone abolished fever response, and the chimeric virus did not show adaptation in rabbits as demonstrated by lack of viral RNA and E2 protein expression in the spleen and weak antibody responses. (springer.com)
  • The expression of classical swine fever virus structural protein E2 gene in tobacco chloroplasts for applying chloroplasts as bioreactors. (semanticscholar.org)
  • This NLS mediates the nuclear accumulation of MxA, whereas the swine Mx1 protein is not located inside nucleus for lack of NLS. (beds.ac.uk)
  • The fluorophore E2 fusion protein was readily detectable in purified virus particles by Western blot and fluorescence microscopy and the particle integrity and morphology was confirmed by cryo electron microscopy. (nature.com)
  • To gain dynamic information of virus entry, viruses can be genetically labelled by the fusion of a structural protein with a fluorophore or by labelling components of intact virus particles with a fluorescent dye (e.g. adenovirus/enveloped particles with DiD) (reviewed in 7 ). (nature.com)
  • A cDNA copy of the viral genomes listed below would not be regulated because they would first need to be transcribed into RNA then translated into protein and therefore would not be an immediate precursor to virus. (cdc.gov)
  • Replacement of E rns , E2, E rns -E2, or E1-E2 of the C-strain with the counterpart(s) of the Shimen strain led to decreased fever response, reduction of viral RNA and antibody responses in rabbits, as compared with their parental C-strain. (springer.com)
  • HoJo virus (HOJOV) A strain of Hantaan virus in the genus Hantavirus, recovered from the blood of a patient with Korean hemorrhagic fever. (rrnursingschool.biz)
  • Genus Tequatrovirus ( T4virus , T4-ähnliche Viren , en. (wikipedia.org)
  • A contributor to Diseases of Swine, 8th edition, Dr. Zimmerman has selected three additional editors with international expertise. (wiley.com)
  • We have versatile animal facilities that allow studies on infectious diseases caused by bacteria, viruses, parasites and prions. (wur.nl)
  • Topics include all priority livestock and aquaculture infectious diseases (including those caused by viruses, bacteria, pests, parasites) and non-infectious diseases. (bovinevetonline.com)
  • Classical swine fever is one of the diseases that will be studied at NBAF, and we're proud to be supporting progress in fighting this economic threat to pork production worldwide. (k-state.edu)
  • The range of clinical signs and its clinical similarity to other diseases can make classical swine fever challenging to diagnose. (usda.gov)
  • Tomlinson JA (1987) Epidemiology and control of virus diseases of vegetables. (springermedizin.de)
  • These two viruses have led to complications in clinical diagnosis. (springer.com)
  • Pestiviruses are small, enveloped, positive-stranded RNA viruses ( 23 ). (asm.org)
  • Dengue virus is an arbovirus transmitted by mosquitoes which causes significant morbidity and mortality among children in tropical regions, especially in resource limited countries. (bioportfolio.com)
  • The mosquito Aedes aegypti carries several arthropod-borne viruses (arboviruses) that are pathogenic to humans, including dengue and Zika viruses. (asm.org)
  • The database (DB) of the European Union (EU) and World Organisation for Animal Health (OIE) Reference Laboratory for classical swine fever offers one of the world's largest semi-public virus-specific sequence collections combined with a module for phylogenetic analysis. (mdpi.com)
  • Avian influenza viruses of the H5 or H7 subtype that do not have either of the characteristics described above should be sequenced to determine whether multiple basic amino acids are present at the cleavage site of the haemagglutinin molecule (HAO). (ufl.edu)
  • A species of gram-negative, rod-shaped bacteria isolated from the intestinal tract of swine, poultry, and man. (bioportfolio.com)
  • Cryo EM structure of the rabies virus ribonucleoprotein complex. (nih.gov)
  • The objective of this project is to understand the immune mechanisms underlying protection against CSF, in particular the mechanisms involved in early protection against clinical signs of CSF and in the reduction of (horizontal) virus transmission. (europa.eu)
  • These processes are associated with the production of antigenic proteins that make the virus vulnerable to immune control mechanisms 'warning' the host of the presence of an invader [ 1 ]. (intechopen.com)
  • During the period of coexistence with their hosts, viruses have learned how to manipulate host immune control mechanism. (intechopen.com)
  • The present review will highlight the different complex mechanisms associated with the host immune evasion by the viruses with special reference to the Classical Swine Fever Virus. (intechopen.com)
  • There are certain cellular components which are manipulated by viruses to evade the innate immune response. (intechopen.com)
  • Indeed, recent studies on CSF indicate an important role for cellmediated immune responses in protection against the virus [31]. (03964.com)
  • However the Classical Swine Fever Virus is able to avoid the innate immune system. (defra.gov.uk)
  • The aim of this proposal is to further our understanding of the mechanisms by which the virus is able to avoid these innate immune response. (defra.gov.uk)
  • Comparison of viraemia- and clinical-based estimates of within- and between-pen transmission of classical swine fever virus from three transmission experiments. (semanticscholar.org)
  • no clinical signs or virus transmission to sentinel animals were observed after 35 days. (bvsalud.org)
  • Unfortunately, due to the fact that PEDV and a different Alphacoronavirus, transmissible gastroenteritis virus (TGEV), have the same epidemiological and clinical features. (springer.com)
  • Introduction of genotype 8 viruses is indicated. (cdc.gov)
  • In addition to the IFN- responses, induction of both CD4+ T helper cell and CD8+ cytotoxic T cells (CTL) were discernible C activation of the latter was confirmed in a virus-specific cytolytic assay. (03964.com)
  • Here we describe an extremely sensitive assay to analyze the effects of mutations on the virus RNA synthesis machinery in cells of both. (asm.org)