Citrobacter rodentium
Citrobacter
Citrobacter freundii
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria found in humans and other animals including MAMMALS; BIRDS; REPTILES; and AMPHIBIANS. It has also been isolated from SOIL and WATER as well as from clinical specimens such as URINE; THROAT; SPUTUM; BLOOD; and wound swabs as an opportunistic pathogen.
Colon
Citrobacter koseri
Colitis
Enteropathogenic Escherichia coli
Strains of ESCHERICHIA COLI characterized by attaching-and-effacing histopathology. These strains of bacteria intimately adhere to the epithelial cell membrane and show effacement of microvilli. In developed countries they are associated with INFANTILE DIARRHEA and infantile GASTROENTERITIS and, in contrast to ETEC strains, do not produce ENDOTOXINS.
Colonic Diseases
Intestinal Mucosa
Rodent Diseases
Hyperplasia
Bacterial Adhesion
Virulence
Escherichia
Adhesins, Bacterial
Cell-surface components or appendages of bacteria that facilitate adhesion (BACTERIAL ADHESION) to other cells or to inanimate surfaces. Most fimbriae (FIMBRIAE, BACTERIAL) of gram-negative bacteria function as adhesins, but in many cases it is a minor subunit protein at the tip of the fimbriae that is the actual adhesin. In gram-positive bacteria, a protein or polysaccharide surface layer serves as the specific adhesin. What is sometimes called polymeric adhesin (BIOFILMS) is distinct from protein adhesin.
Immunity, Mucosal
Nonsusceptibility to the pathogenic effects of foreign microorganisms or antigenic substances as a result of antibody secretions of the mucous membranes. Mucosal epithelia in the gastrointestinal, respiratory, and reproductive tracts produce a form of IgA (IMMUNOGLOBULIN A, SECRETORY) that serves to protect these ports of entry into the body.
Mice, Knockout
Strains of mice in which certain GENES of their GENOMES have been disrupted, or "knocked-out". To produce knockouts, using RECOMBINANT DNA technology, the normal DNA sequence of the gene being studied is altered to prevent synthesis of a normal gene product. Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases (DISEASE MODELS, ANIMAL) and to clarify the functions of the genes.
Virulence Factors
Those components of an organism that determine its capacity to cause disease but are not required for its viability per se. Two classes have been characterized: TOXINS, BIOLOGICAL and surface adhesion molecules that effect the ability of the microorganism to invade and colonize a host. (From Davis et al., Microbiology, 4th ed. p486)
Methane
Mesna
Methanosarcina
A genus of anaerobic, irregular spheroid-shaped METHANOSARCINALES whose organisms are nonmotile. Endospores are not formed. These archaea derive energy via formation of methane from acetate, methanol, mono-, di-, and trimethylamine, and possibly, carbon monoxide. Organisms are isolated from freshwater and marine environments.
Methanosarcinales
Euryarchaeota
Trinidad and Tobago
An independent state in the Lesser Antilles in the West Indies, north of Venezuela, comprising the islands of Trinidad and Tobago. Its capital is Port of Spain. Both islands were discovered by Columbus in 1498. The Spanish, English, Dutch, and French figure in their history over four centuries. Trinidad and Tobago united in 1898 and were made part of the British colony of Trinidad and Tobago in 1899. The colony became an independent state in 1962. Trinidad was so named by Columbus either because he arrived on Trinity Sunday or because three mountain peaks suggested the Holy Trinity. Tobago was given the name by Columbus from the Haitian tambaku, pipe, from the natives' habit of smoking tobacco leaves. (Webster's New Geographical Dictionary, 1988, p1228, 1216 & Room, Brewer's Dictionary of Names, 1992, p555, 547)
Osteopontin
Epithelial Cells
Cells that line the inner and outer surfaces of the body by forming cellular layers (EPITHELIUM) or masses. Epithelial cells lining the SKIN; the MOUTH; the NOSE; and the ANAL CANAL derive from ectoderm; those lining the RESPIRATORY SYSTEM and the DIGESTIVE SYSTEM derive from endoderm; others (CARDIOVASCULAR SYSTEM and LYMPHATIC SYSTEM) derive from mesoderm. Epithelial cells can be classified mainly by cell shape and function into squamous, glandular and transitional epithelial cells.
MicroRNAs
Small double-stranded, non-protein coding RNAs, 21-25 nucleotides in length generated from single-stranded microRNA gene transcripts by the same RIBONUCLEASE III, Dicer, that produces small interfering RNAs (RNA, SMALL INTERFERING). They become part of the RNA-INDUCED SILENCING COMPLEX and repress the translation (TRANSLATION, GENETIC) of target RNA by binding to homologous 3'UTR region as an imperfect match. The small temporal RNAs (stRNAs), let-7 and lin-4, from C. elegans, are the first 2 miRNAs discovered, and are from a class of miRNAs involved in developmental timing.
Critical role of T cell-dependent serum antibody, but not the gut-associated lymphoid tissue, for surviving acute mucosal infection with Citrobacter rodentium, an attaching and effacing pathogen. (1/177)
Citrobacter rodentium uses virulence factors similar to the enteropathogenic Escherichia coli to produce attaching and effacing lesions in the distal colon of mice. We used this infection model to determine components of adaptive immunity needed to survive infection. During acute infection, wild-type mice develop breaks across infected epithelial surfaces but resolve infection. Surprisingly, mice markedly deficient in mucosal lymphocyte populations from beta(7) integrin deficiency resolve infection, as do CD8alpha-/- or TCR-delta-/- mice. In contrast, CD4-/- or TCR-beta-/- mice develop polymicrobial sepsis and end-organ damage, and succumb during acute infection, despite epithelial damage similar to wild-type mice. B cell-deficient (MuMT-/-) or B and T cell-deficient (recombinase-activating gene 2-/-) mice develop severe pathology in colon and internal organs, and deteriorate rapidly during acute infection. Surviving mice develop robust Citrobacter-specific serum IgM responses during acute infection, whereas mice that succumb do not. However, CD4-/- mice receiving serum Igs from infected wild-type mice survive and clear the infection. Our data show that survival of apparently self-limited and luminal mucosal infections requires a systemic T cell-dependent Ab response against bacteria that enter through damaged mucosa. These findings have implications for understanding host defense against mucosal infections, including the pathogenesis of these diseases in immunocompromised populations. (+info)Dissecting virulence: systematic and functional analyses of a pathogenicity island. (2/177)
Bacterial pathogenicity islands (PAI) often encode both effector molecules responsible for disease and secretion systems that deliver these effectors to host cells. Human enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. coli, and the mouse pathogen Citrobacter rodentium (CR) possess the locus of enterocyte effacement (LEE) PAI. We systematically mutagenized all 41 CR LEE genes and functionally characterized these mutants in vitro and in a murine infection model. We identified 33 virulence factors, including two virulence regulators and a hierarchical switch for type III secretion. In addition, 7 potential type III effectors encoded outside the LEE were identified by using a proteomics approach. These non-LEE effectors are encoded by three uncharacterized PAIs in EHEC O157, suggesting that these PAIs act cooperatively with the LEE in pathogenesis. Our findings provide significant insights into bacterial virulence mechanisms and disease. (+info)Identification of a novel Citrobacter rodentium type III secreted protein, EspI, and roles of this and other secreted proteins in infection. (3/177)
Citrobacter rodentium is a member of a group of pathogens that colonize the lumen of the host gastrointestinal tract via attaching and effacing (A/E) lesion formation. C. rodentium, which causes transmissible colonic hyperplasia in mice, is used as an in vivo model system for the clinically significant A/E pathogens enterohemorrhagic and enteropathogenic Escherichia coli. These bacteria all contain a pathogenicity island called the locus of enterocyte effacement (LEE), which encodes a type III secretion system that is designed to deliver effector proteins into eukaryotic host cells. These effectors are involved in the subversion of host eukaryotic cell functions to the benefit of the bacterium. In this study we used mutant strains to determine the effects of the C. rodentium LEE-encoded effectors EspF, EspG, EspH, and Map on virulence in the mouse model. In addition, we identified a novel secreted protein, EspI encoded outside the LEE, whose secretion is also dependent on a functional type III secretion system. Mutant strains with each of the effectors investigated were found to be outcompeted by wild-type bacteria in mixed-infection experiments in vivo, although the effects of EspF and EspH were only subtle. In single-infection experiments, we found that EspF, EspG, and EspH are not required for efficient colonization of the mouse colon or for the production of hyperplasia. In contrast, strains producing EspI and Map had significant colonization defects and resulted in dramatically reduced levels of hyperplasia, and they exhibited very different growth dynamics in mice than the wild-type strain exhibited. (+info)Clearance of Citrobacter rodentium requires B cells but not secretory immunoglobulin A (IgA) or IgM antibodies. (4/177)
Citrobacter rodentium, a murine model pathogen for human enteropathogenic Escherichia coli, predominantly colonizes the lumen and mucosal surface of the colon and cecum and causes crypt hyperplasia and mucosal inflammation. Mice infected with C. rodentium develop a secretory immunoglobulin A (IgA) response, but the role of B cells or secretory antibodies in host defense is unknown. To address this question, we conducted oral C. rodentium infections in mice lacking B cells, IgA, secreted IgM, polymeric Ig receptor (pIgR), or J chain. Normal mice showed peak bacterial numbers in colon and feces at 1 week and bacterial eradication after 3 to 4 weeks. B-cell-deficient mice were equally susceptible initially but could not control infection subsequently. Tissue responses showed marked differences, as infection of normal mice was accompanied by transient crypt hyperplasia and mucosal inflammation in the colon and cecum at 2 but not 6 weeks, whereas B-cell-deficient mice had few mucosal changes at 2 weeks but severe epithelial hyperplasia with ulcerations and mucosal inflammation at 6 weeks. The functions of B cells were not mediated by secretory antibodies, since mice lacking IgA or secreted IgM or proteins required for their transport into the lumen, pIgR or J chain, cleared C. rodentium normally. Nonetheless, systemic administration of immune sera reduced bacterial numbers significantly in normal and pIgR-deficient mice, and depletion of IgG abrogated this effect. These results indicate that host defense against C. rodentium depends on B cells and IgG antibodies but does not require production or transepithelial transport of IgA or secreted IgM. (+info)Protective role of arginase in a mouse model of colitis. (5/177)
Arginase is the endogenous inhibitor of inducible NO synthase (iNOS), because both enzymes use the same substrate, l-arginine (Arg). Importantly, arginase synthesizes ornithine, which is metabolized by the enzyme ornithine decarboxylase (ODC) to produce polyamines. We investigated the role of these enzymes in the Citrobacter rodentium model of colitis. Arginase I, iNOS, and ODC were induced in the colon during the infection, while arginase II was not up-regulated. l-Arg supplementation of wild-type mice or iNOS deletion significantly improved colitis, and l-Arg treatment of iNOS(-/-) mice led to an additive improvement. There was a significant induction of IFN-gamma, IL-1, and TNF-alpha mRNA expression in colitis tissues that was markedly attenuated with l-Arg treatment or iNOS deletion. Treatment with the arginase inhibitor S-(2-boronoethyl)-l-cysteine worsened colitis in both wild-type and iNOS(-/-) mice. Polyamine levels were increased in colitis tissues, and were further increased by l-Arg. In addition, in vivo inhibition of ODC with alpha-difluoromethylornithine also exacerbated the colitis. Taken together, these data indicate that arginase is protective in C. rodentium colitis by enhancing the generation of polyamines in addition to competitive inhibition of iNOS. Modulation of the balance of iNOS and arginase, and of the arginase-ODC metabolic pathway may represent a new strategy for regulating intestinal inflammation. (+info)Macroscopic, microscopic and biochemical characterisation of spontaneous colitis in a transgenic mouse, deficient in the multiple drug resistance 1a gene. (6/177)
1 A novel animal model of spontaneous colonic inflammation, the multiple drug-resistant (mdr1) a(-/-) mouse, was identified by Panwala and colleagues in 1998. The aim of our study was to further characterise this model, specifically by measuring cytokines that have been implicated in inflammatory bowel disease (IBD) (IL-8 and IFN-gamma) in the colon/rectum of mdr1a(-/-) mice, and by determining the sensitivity of these, together with the macroscopic, microscopic and disease signs of colitis, to dexamethasone (0.05, 0.3 and 2 mg kg(-1) subcutaneously (s.c.) daily for 7 days). 2 All mdr1a(-/-) mice had microscopic evidence of inflammation in the caecum and colon/rectum, while control mice with the same genetic background did not. Significant increases in colon/rectum and caecal weights and also in cytokine levels (both IFN-gamma and IL-8) in homogenised colon/rectum were observed in mdr1a(-/-) mice compared to mdr1a(+/+) mice. 3 Dexamethasone reduced the increases in tissue weights and also microscopic grading of colitis severity, but had no effect on IFN-gamma or IL-8. 4 This study supports the similarity of the gastrointestinal inflammation present in mdr1a(-/-) mice to that of human IBD, in particular Crohn's disease. This has been demonstrated at the macroscopic and microscopic levels, and was supported further by elevations in colonic levels of IFN-gamma and IL-8 and the disease signs observed. The incidence of colitis was much higher than previously reported, with all mice having microscopic evidence of colitis. The limited variance between animals in the parameters measured suggests that this model is reproducible. (+info)Impaired immunity to intestinal bacterial infection in stromelysin-1 (matrix metalloproteinase-3)-deficient mice. (7/177)
Infection of mice with the intestinal bacterial pathogen Citrobacter rodentium results in colonic mucosal hyperplasia and a local Th1 inflammatory response similar to that seen in mouse models of inflammatory bowel disease. Matrix metalloproteinases (MMPs) have been shown to mediate matrix remodeling and cell migration during tissue injury and repair in the intestine. We have previously shown enhanced pathology in infected TNFRp55-/-, IL-12p40-/-, and IFN-gamma-/- mice, and here we show that this is associated with an increase in stromelysin-1 (MMP3) transcripts in colonic tissues. We have therefore investigated the role of MMP3 in colonic mucosal hyperplasia and the local Th1 responses using MMP3-/- mice. In MMP3-/- mice, similar mucosal thickening was observed after infection as in wild-type (WT) mice. Colonic tissues from MMP3-/- mice showed a compensatory increase in the expression of other MMP transcripts, such as MMP7 and MMP12. However, MMP3-/- mice showed delayed clearance of bacteria and delayed appearance of CD4+ T lymphocytes into intestinal lamina propria. CSFE-labeled mesenteric lymph node CD4+ T lymphocytes from infected WT mice migrated in fewer numbers into the mesenteric lymph nodes and colon of MMP3-/- mice than into those of WT mice. These studies show that mucosal remodeling can occur in the absence of MMP3, but that MMP3 plays a role in the migration of CD4+ T lymphocytes to the intestinal mucosa. (+info)EspJ is a prophage-carried type III effector protein of attaching and effacing pathogens that modulates infection dynamics. (8/177)
Enterohemorrhagic Escherichia coli, enteropathogenic E. coli, and Citrobacter rodentium are highly adapted enteropathogens that successfully colonize their host's gastrointestinal tract via the formation of attaching and effacing (A/E) lesions. These pathogens utilize a type III secretion system (TTSS) apparatus, encoded by the locus of enterocyte effacement, to translocate bacterial effector proteins into epithelial cells. Here, we report the identification of EspJ (E. coli-secreted protein J), a translocated TTSS effector that is carried on the 5' end of the cryptic prophage CP-933U. Infection of epithelial cells in culture revealed that EspJ is not required for A/E lesion activity in vivo and ex vivo. However, in vivo studies performed with mice demonstrated that EspJ possesses properties that influence the dynamics of clearance of the pathogen from the host's intestinal tract, suggesting a role in host survival and pathogen transmission. (+info)
Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice<...
Citrobacter rodentium subverts ATP flux and cholesterol homeostasis in intestinal epithelial cells in vivo -ORCA
The Sphingosine-1-Phosphate Analogue FTY720 Impairs Mucosal Immunity and Clearance of the Enteric Pathogen Citrobacter...
The enterohemorrhagic Escherichia coli effector protein NleF binds mammalian Tmp21
Osteopontin mediates Citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions<...
Permalien vers The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and...
Requirement of epithelial integrin-linked kinase for facilitation of Citrobacter rodentium-induced colitis | BMC...
Mucin Dynamics in Intestinal Bacterial Infection
Search Results | RUP
British Library EThOS: Subversion of Rho GTPases by WxxxE effectors of attaching and effacing pathogens
Publications - Professor Gad Frankel
Browsing Dept. of Experimentelle Immunologie (EXIM) by Issue Date
Role of Class 1 Serine Protease Autotransporter in the Pathogenesis of Citrobacter rodentium Colitis
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4WKM AMPR EFFECTOR BINDING DOMAIN FROM CITROBACTER FREUNDII BOUND TO UDP- MURNAC-PENTAPEPTIDE | 4WKM I | P15056 | BRAF | Serine...
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Inflammasome
Salmonella and Citrobacter rodentium. Intracellular bacteria trigger activation of the inflammasome, which results in specific ...
AP-1 transcription factor
... independent inflammatory responses following infection by enteropathogenic Escherichia coli and Citrobacter rodentium". ...
Type three secretion system
... inhibitor of the bacterial type III secretion system protects against in vivo infection with Citrobacter rodentium". The ...
Colonization resistance
... coli directly competes with Citrobacter rodentium for carbohydrates in the intestinal lumen) or by producing growth inhibitors ...
Hepatitis D bahasa Indonesia, ensiklopedia bebas
"Infection with enteric pathogens Salmonella typhimurium and Citrobacter rodentium modulate TGF-beta/Smad signaling pathways in ...
Intimin
... in EPEC endemic areas The serum of EPEC/EHEC infected children and EPEC infected volunteers Secretions of Citrobacter rodentium ...
6-Formylindolo(3,2-b)carbazole
... and they do not survive infection by the intestinal pathogen Citrobacter rodentium. The same authors reported that in wild-type ... that overexpress Cyp1a1 in the gut epithelium led to a pseudo-AHR-deficient state and when infected with Citrobacter rodentium ...
List of MeSH codes (B03)
Citrobacter freundii MeSH B03.440.450.425.200.475 - Citrobacter koseri MeSH B03.440.450.425.200.737 - Citrobacter rodentium ... Citrobacter freundii MeSH B03.660.250.150.100.475 - Citrobacter koseri MeSH B03.660.250.150.100.737 - Citrobacter rodentium ...
Innate lymphoid cell
... such as gastrointestinal Citrobacter rodentium infection. Retinoic acid also enhances the expression of gut- homing markers on ...
Citrobacter rodentium
"Citrobacter rodentium" at the Encyclopedia of Life LPSN Type strain of Citrobacter rodentium at BacDive - the Bacterial ... Citrobacter rodentium is a Gram-negative species of bacteria. Schauer DB, Zabel BA, Pedraza IF, O'Hara CM, Steigerwalt AG, ... Mundy R, MacDonald TT, Dougan G, Frankel G, Wiles S (2005). "Citrobacter rodentium of mice and man". Cell Microbiol. 7 (12): ... Bhinder G, Sham HP, Chan JM, Morampudi V, Jacobson K, Vallance BA (2013). "The Citrobacter rodentium mouse model: studying ...
Citrobacter rodentium - Wikipedia
"Citrobacter rodentium" at the Encyclopedia of Life LPSN Type strain of Citrobacter rodentium at BacDive - the Bacterial ... Citrobacter rodentium is a Gram-negative species of bacteria. Schauer DB, Zabel BA, Pedraza IF, OHara CM, Steigerwalt AG, ... Mundy R, MacDonald TT, Dougan G, Frankel G, Wiles S (2005). "Citrobacter rodentium of mice and man". Cell Microbiol. 7 (12): ... Bhinder G, Sham HP, Chan JM, Morampudi V, Jacobson K, Vallance BA (2013). "The Citrobacter rodentium mouse model: studying ...
Citrobacter rodentium: infection, inflammation and the microbiota
Thus, C. rodentium has long been used as a model to … ... Citrobacter rodentium is a mucosal pathogen of mice that shares ... Citrobacter rodentium: infection, inflammation and the microbiota Nat Rev Microbiol. 2014 Sep;12(9):612-23. doi: 10.1038/ ... Citrobacter rodentium is a mucosal pathogen of mice that shares several pathogenic mechanisms with enteropathogenic Escherichia ... Thus, C. rodentium has long been used as a model to understand the molecular basis of EPEC and EHEC infection in vivo. In this ...
KEGG PATHWAY: Methane metabolism - Citrobacter rodentium
Molecular pathogenesis of Citrobacter rodentium and transmissible murine colonic hyperplasia
... pathology and molecular pathogenesis of Citrobacter rodentium, the causative agent of transmissible murine colonic hyperplasia ... C. rodentium serves as an important model pathogen for investigating the mechanisms controlling attaching and effacing pa … ... Molecular pathogenesis of Citrobacter rodentium and transmissible murine colonic hyperplasia Microbes Infect. 2001 Apr;3(4):333 ... Here we review the history, clinical significance, pathology and molecular pathogenesis of Citrobacter rodentium, the causative ...
Citrobacter rodentium | Harvard Catalyst Profiles | Harvard Catalyst
"Citrobacter rodentium" by people in Harvard Catalyst Profiles by year, and whether "Citrobacter rodentium" was a major or minor ... "Citrobacter rodentium" is a descriptor in the National Library of Medicines controlled vocabulary thesaurus, MeSH (Medical ... Virulence factors enhance Citrobacter rodentium expansion through aerobic respiration. Science. 2016 09 16; 353(6305):1249-53. ... Below are the most recent publications written about "Citrobacter rodentium" by people in Profiles. ...
Dietary Chitosan Supplementation Increases Microbial Diversity and Attenuates the Severity of Citrobacter rodentium Infection...
Citrobacter rodentium, a mucosal pathogen found in mice, and enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E ... After Citrobacter rodentium infection, the histopathology scores indicated that COS feeding resulted in less severe colitis. IL ... R. Mundy, T. T. MacDonald, G. Dougan, G. Frankel, and S. Wiles, "Citrobacter rodentium of mice and man," Cellular Microbiology ... S. A. Luperchio, J. V. Newman, C. A. Dangler et al., "Citrobacter rodentium, the causative agent of transmissible murine ...
Irgm1-deficiency leads to myeloid dysfunction in colon lamina propria and susceptibility to the intestinal pathogen Citrobacter...
Here we find that loss of Irgm1 confers marked susceptibility to Citrobacter rodentium, a noninvasive intestinal pathogen that ... rodentium infection and are essential for C. rodentium immunity. Defective immune remodeling was most striking in C-LP ... leads to myeloid dysfunction in colon lamina propria and susceptibility to the intestinal pathogen Citrobacter rodentium. * ... Irgm1-deficient mice fail to control C. rodentium outgrowth in the intestine, leading to systemic pathogen spread and host ...
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Citrobacter rodentium induced liver changes in C57BL/6 mice : animal model of acute inflammatory...
Citrobacter rodentium induced liver changes in C57BL/6 mice : animal model of acute inflammatory stress and injury. Download ... Citrobacter rodentium induced liver changes in C57BL/6 mice : animal model of acute inflammatory stress and injury. Research ... Using Citrobacter rodentium, a well characterized rodent-specific enteric pathogen as a source of extrahepatic inflammatory ... C. rodentium-induced inflammatory stress was finally investigated for its potential in altering drug pharmacokinetics (PK) of ...
Frontiers | Egg Protein Transferrin-Derived Peptides IRW and IQW Regulate Citrobacter rodentium-Induced, Inflammation-Related...
Forty-eight mice were randomly assigned to six groups: basal diet (CTRL), intragastric administration Citrobacter rodentium (CR ... Forty-eight mice were randomly assigned to six groups: basal diet (CTRL), intragastric administration Citrobacter rodentium (CR ... to treat enteritis in a mouse model of Citrobacter rodentium-induced colitis by evaluating serum metabolomics and gut microbes ... to treat enteritis in a mouse model of Citrobacter rodentium-induced colitis by evaluating serum metabolomics and gut microbes ...
Unpacking the Unknown: Characterisation of Citrobacter rodentium extracellular vesicles
Citrobacter rodentium is a murine enteropathogen which infects mice using the same modus operandi as EPEC and EHEC, allowing ... Unpacking the Unknown: Characterisation of Citrobacter rodentium extracellular vesicles. ResearchSpace/Manakin Repository. ... As prior studies have shown EV production by E. coli, this begs the question of whether the same is true for C. rodentium, ... My results show that the concentration of EVs produced by C. rodentium in both rich and defined media is low compared to that ...
Citrobacter rodentium Infection in Mice Elicits a Mucosal Th1 Cytokine Response and Lesions Similar to Those in Murine...
Citrobacter rodentium, formerly Citrobacter freundii biotype 4280, causes transmissible murine colonic hyperplasia (6, 45, 46 ... Citrobacter rodentium Infection in Mice Elicits a Mucosal Th1 Cytokine Response and Lesions Similar to Those in Murine ... Citrobacter rodentium Infection in Mice Elicits a Mucosal Th1 Cytokine Response and Lesions Similar to Those in Murine ... Citrobacter rodentium Infection in Mice Elicits a Mucosal Th1 Cytokine Response and Lesions Similar to Those in Murine ...
The p50 Subunit of NF-κB Is Critical for In Vivo Clearance of the Noninvasive Enteric Pathogen Citrobacter rodentium |...
The p50 Subunit of NF-κB Is Critical for In Vivo Clearance of the Noninvasive Enteric Pathogen Citrobacter rodentium. Alison ... In contrast, Citrobacter rodentium is a natural mouse pathogen that shares many virulence factors with EPEC and EHEC and relies ... Clearance of Citrobacter rodentium requires B cells but not secretory immunoglobulin A (IgA) or IgM antibodies. Infect. Immun. ... Citrobacter rodentium infection in mice elicits a mucosal Th1 cytokine response and lesions similar to those in murine ...
Selective Modulation of Hepatic Cytochrome P450 and Flavin Monooxygenase 3 Expression during Citrobacter rodentium Infection in...
Citrobacter rodentium. Fmo. flavin monooxygenase. TLR. Toll-like receptor. TNF. tumor necrosis factor. IL. interleukin. IFNγ. ... Selective Modulation of Hepatic Cytochrome P450 and Flavin Monooxygenase 3 Expression during Citrobacter rodentium Infection in ... Selective Modulation of Hepatic Cytochrome P450 and Flavin Monooxygenase 3 Expression during Citrobacter rodentium Infection in ... gene expression caused by infection with the murine intestinal pathogen Citrobacter rodentium has been well characterized in ...
Role of Class 1 Serine Protease Autotransporter in the Pathogenesis of Citrobacter rodentium Colitis
Influence of NleH effector expression, host genetics, and inflammation on Citrobacter rodentium colonization of mice
The mouse pathogen Citrobacter rodentium encodes one NleH protein, which functions equivalently to E. coli NleH1. We examined ... Influence of NleH effector expression, host genetics, and inflammation on Citrobacter rodentium colonization of mice. en_US. ... Influence of NleH effector expression, host genetics, and inflammation on Citrobacter rodentium colonization of mice. K-REx ... C. rodentium that expressed both E. coli NleH1 and NleH2 was hypervirulent in C3H/HeJ mice.. en_US. ...
The Citrobacter rodentium type III secretion system effector EspO affects mucosal damage repair and antimicrobial responses -...
In order to colonize the host and compete with the gut microbiota, C. rodentium employs a type III secretion system (T3SS) that ... Importantly, while the reduced CCH and abundance of antimicrobial proteins during ΔespO infection did not affect C. rodentium ... Of the main hallmarks of C. rodentium infection are colonic crypt hyperplasia (CCH) and dysbiosis. ... rodentium infection as well as secretion of IL-22 from colonic explants. While we observed no differences in the recruitment of ...
Intimin-specific immune responses prevent bacterial colonization by the attaching-effacing pathogen Citrobacter rodentium -...
Citrobacter rodentium mouse model of bacterial infection. - LSHTM Research Online | London School of Hygiene and Tropical...
Crepin, VF; Collins, JW; Habibzay, M; Frankel, G; (2016) Citrobacter rodentium mouse model of bacterial infection. Nature ... Infection of mice with Citrobacter rodentium is a robust model to study bacterial pathogenesis, mucosal immunology, the health ... C. rodentium was first isolated by Barthold from an outbreak of mouse diarrhea in Yale University in 1972 and was rediscovered ...
RegA, an AraC-Like Protein, Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in Citrobacter...
... is-a-global-transcriptional-regulator-that-controls-virulence-gene-expression-in-citrobacter-rodentium. ... Is a Global Transcriptional Regulator That Controls Virulence Gene Expression in Citrobacter rodentium. ... We thank Rosanna Mundy (Imperial College, London, United Kingdom) for constructing the STM library of C. rodentium, Judyta ...
Fermented dairy products modulate Citrobacter rodentium-induced colonic hyperplasia. - LSHTM Research Online | London School...
S100A4 contributes to colitis development by increasing the adherence of Citrobacter rodentium in intestinal epithelial cells |...
rodentium infection led to a rapid increase of these cells. There were more S100A4+ cells in the C. rodentium affected colon on ... rodentium-infected mouse colons. It is still not clear whether S100A4 is expressed in the mouse colon during C. rodentium ... Here, upon infection with Citrobacter rodentium, a model for enteropathogenic Escherichia coli infection in humans, induced the ... rodentium in 200 μl PBS. Various tissues were collected both prior to and during C. rodentium infection. The mRNA expression of ...
Probiotics prevent death caused by Citrobacter rodentium Infection in neonatal mice<...
Gareau, M., Wine, E., Reardon, C., & Sherman, P. M. (2010). Probiotics prevent death caused by Citrobacter rodentium Infection ... Probiotics prevent death caused by Citrobacter rodentium Infection in neonatal mice. Melanie Gareau, Eytan Wine, Colin Reardon ... Gareau, M, Wine, E, Reardon, C & Sherman, PM 2010, Probiotics prevent death caused by Citrobacter rodentium Infection in ... Probiotics prevent death caused by Citrobacter rodentium Infection in neonatal mice. / Gareau, Melanie; Wine, Eytan; Reardon, ...
Osteopontin mediates Citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions<...
Osteopontin mediates Citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions. / Wine, ... Osteopontin mediates Citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions. American ... title = "Osteopontin mediates Citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions ... T1 - Osteopontin mediates Citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions ...
Citrobacter rodentium subverts ATP flux and cholesterol homeostasis in intestinal epithelial cells in vivo -ORCA
Citrobacter rodentium subverts ATP flux and cholesterol homeostasis in intestinal epithelial cells in vivo. Cell Metabolism 26 ... Citrobacter rodentium subverts ATP flux and cholesterol homeostasis in intestinal epithelial cells in vivo ... We investigated the impact of infection with the enteric pathogen Citrobacter rodentium on mouse IEC metabolism using global ... These results suggest that C. rodentium manipulates host metabolism to evade innate immune responses and establish a favorable ...
Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice<...
Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice. Simon Clare, Victoria John, Alan W ... Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice. Infection and Immunity. 2013 Mar;81( ... Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice. In: Infection and Immunity. 2013 ; ... Enhanced susceptibility to Citrobacter rodentium infection in microRNA-155-deficient mice. / Clare, Simon; John, Victoria; ...
Identification of a novel type IV pilus gene cluster required for gastrointestinal colonization of Citrobacter rodentium. -...
... rodentium (named colonization factor Citrobacter, CFC). The proteins encoded by cfc genes have identity to proteins of the type ... rodentium was constructed and screened in mice. Of the 576 mutants tested, 14 were attenuated in their ability to colonize the ... rodentium. Thus, CFC provides a convenient model to study type IV pilus-mediated pathogen-host interactions under physiological ... Citrobacter rodentium is used as an in vivo model system for clinically significant enteric pathogens such as ...
Streptococcus pneumoniae-induced pneumonia and citrobacter rodentium-induced gut infection differentially alter vitamin A...
Fingerprint Dive into the research topics of Streptococcus pneumoniae-induced pneumonia and citrobacter rodentium-induced gut ... T1 - Streptococcus pneumoniae-induced pneumonia and citrobacter rodentium-induced gut infection differentially alter vitamin A ... Streptococcus pneumoniae-induced pneumonia and citrobacter rodentium-induced gut infection differentially alter vitamin A ... Streptococcus pneumoniae-induced pneumonia and citrobacter rodentium-induced gut infection differentially alter vitamin A ...
The NF-κB transcription factor c-Rel controls host defense against Citrobacter rodentium.
CD4+ T cells drive goblet cell depletion during Citrobacter rodentium infection. * Authors: Chan JM, Bhinder G, Sham HP, Ryz N ... Mice lacking CD4+ T cells or B cells are highly susceptible to Citrobacter rodentium infection. In this study, we show that the ... The p50 subunit of NF-kappaB is critical for in vivo clearance of the noninvasive enteric pathogen Citrobacter rodentium. ... Gamma interferon produced by antigen-specific CD4+ T cells regulates the mucosal immune responses to Citrobacter rodentium ...
nature.com search
Pathogen Citrobacter rodentiumColonizationColitisEscherichiaHyperplasiaPathogenesisEHECInflammationComplete Genome Sequence of CitrobacterC57BLSusceptibilityType III secretion sMicrobiotaEntericGastrointestinalSalmonellaFreundii biotypeEffectorPathogenicityColiResponsesStrainBacteriaSeverityNoninvasiveSystemicMurine modelIntestinal epithelial cellsWerkmaniiEpithelial cellWild-type miceVivoMousePhenotypeMice infectedDysbiosisTissue damageHost
Pathogen Citrobacter rodentium10
- Irgm1-deficiency leads to myeloid dysfunction in colon lamina propria and susceptibility to the intestinal pathogen Citrobacter rodentium. (doaj.org)
- The mouse pathogen Citrobacter rodentium encodes one NleH protein, which functions equivalently to E. coli NleH1. (k-state.edu)
- We evaluated the protective effects of fermented dairy products (FDPs) in an infection model, using the mouse pathogen Citrobacter rodentium (CR). (lshtm.ac.uk)
- The objective of this study was to determine the role of OPN in host responses to a non-invasive bacterial pathogen, Citrobacter rodentium, which serves as a murine infectious model of colitis. (elsevier.com)
- We investigated the impact of infection with the enteric pathogen Citrobacter rodentium on mouse IEC metabolism using global proteomic and targeted metabolomics and lipidomics. (cf.ac.uk)
- Central role for B lymphocytes and CD4+ T cells in immunity to infection by the attaching and effacing pathogen Citrobacter rodentium. (helmholtz-hzi.de)
- Human enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. coli , and the mouse pathogen Citrobacter rodentium (CR) possess the locus of enterocyte effacement (LEE) PAI. (pnas.org)
- To determine if pst contributes to bacterial colonization in vivo, a pstCA mutation was made in the EPEC-like murine pathogen, Citrobacter rodentium. (monash.edu)
- The team infected mice with the murine pathogen Citrobacter rodentium , which establishes first in the cecum. (mentalfloss.com)
- This was tested in vivo using the mouse pathogen Citrobacter rodentium (encoding 31 effectors). (medworm.com)
Colonization18
- In this Review, we discuss recent studies in which C. rodentium has been used to study mucosal immunology, including the deregulation of intestinal inflammatory responses during bacteria-induced colitis and the role of the intestinal microbiota in mediating resistance to colonization by enteric pathogens. (nih.gov)
- Citrobacter rodentium , a mucosal pathogen found in mice, and enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC), enteric pathogens found in humans, create attaching and effacing (A/E) lesions that result in the colonization of mucosa within the intestinal tract [ 1 , 2 ]. (hindawi.com)
- None of the changes were seen in mice inoculated with bacteria lacking intimin (which is necessary for colonization), but they were seen in mice inoculated with C. rodentium complemented with intimin from EPEC. (asm.org)
- C. rodentium shares many virulence factors with EPEC and EHEC and relies on attaching-and-effacing lesion formation for colonization and infection of the gut. (asm.org)
- In contrast, Citrobacter rodentium is a natural mouse pathogen that shares many virulence factors with EPEC and EHEC and relies on A/E lesion formation for colonization and infection of the murine gastrointestinal mucosa ( 22 ). (asm.org)
- Female C57BL/6 (B6) and SCID mice were infected orally with C. rodentium and assessed for bacterial colonization/translocation and P450 and flavin monooxygenase-3 (Fmo3) expression levels after 7 days. (aspetjournals.org)
- We examined the impact of host genetics and intestinal inflammation on the contribution of NleH to C. rodentium colonization of mice differing in LPS responsiveness. (k-state.edu)
- Importantly, while the reduced CCH and abundance of antimicrobial proteins during ΔespO infection did not affect C. rodentium colonization or the composition of commensal Proteobacteria, they had a subtle consequence on Firmicutes subpopulations. (inserm.fr)
- Therefore, these data indicate a novel mechanism for S100A4 that promotes colitis development by enhancing host adhesion and colonization of Citrobacter rodentium through the S100A4-mediated host inflammatory responses. (medicalrecords.com)
- Therefore, lack of OPN results in decreased pedestal formation, colonization, and colonic epithelial cell hyperplasia responses to C. rodentium infection, indicating that OPN impacts disease pathogenesis through bacterial attachment and altered host immune responses. (elsevier.com)
- miR-155-deficient mice exhibited prolonged colonization associated with a higher C. rodentium burden in gastrointestinal tissue and spread into systemic tissues. (elsevier.com)
- Identification of a novel type IV pilus gene cluster required for gastrointestinal colonization of Citrobacter rodentium. (ox.ac.uk)
- In order to identify genes required for the colonization of A/E-forming pathogens, a library of signature-tagged transposon mutants of C. rodentium was constructed and screened in mice. (ox.ac.uk)
- The region flanking the transposon insertion was sequenced, identifying a cluster of 12 genes that encode the first described pilus of C. rodentium (named colonization factor Citrobacter, CFC). (ox.ac.uk)
- A non-polar mutation in cfcI, complementation of this strain with wild-type cfcI and complementation of strain P5H2 with wild-type cfcH confirmed that these genes are required for colonization of the gastrointestinal tract by C. rodentium. (ox.ac.uk)
- This was not due to reduced colonization, but was associated with an altered pattern of C. rodentium bacterial migration. (biomedcentral.com)
- ILK influences the host response to C. rodentium -induced infection, independently of reduced colonization in the ILK knockout mice. (biomedcentral.com)
- This loss of tolerance in monocytes results in greater colonization resistance towards Citrobacter rodentium. (uni-koeln.de)
Colitis10
- After Citrobacter rodentium infection, the histopathology scores indicated that COS feeding resulted in less severe colitis. (hindawi.com)
- A number of significant intestinal disorders found in humans are also frequently modelled by C. rodentium in mice, such as colon tumorigenesis, ulcerative colitis, and Crohn's disease [ 5 ]. (hindawi.com)
- Colitis develops in mice that have been infected with C. rodentium , which results in the bacteria becoming overabundant and the mice's natural microbiota reducing in variety and quantity [ 6 ]. (hindawi.com)
- The genetic makeup of the mouse impacts the formation of C. rodentium -induced colitis, with mice such as C3H/HeJ and FVB/N being prone to develop colitis as a result of the infection and mice such as CD-1 and C57BL/6 being considered amongst those that are not prone to develop colitis and are only prone to subclinical symptoms [ 9 ]. (hindawi.com)
- This study aimed to evaluate the abilities of two peptides derived from egg albumin transferrin, IRW and IQW, to treat enteritis in a mouse model of Citrobacter rodentium -induced colitis by evaluating serum metabolomics and gut microbes. (frontiersin.org)
- Therefore, infection of mice with C . rodentium is an excellent in vivo model of colitis. (medicalrecords.com)
- For this purpose, we used S100A4 knock out ( S100A4 −/− ) and wild-type (WT) mice orally challenged with C . rodentium to establish a model for studying the role of S100A4 and its related molecular mechanism in colitis. (medicalrecords.com)
- Moreover, the pro-resolving properties of KO were validated using a Th1-inducing Citrobacter rodentium colitis murine model. (usda.gov)
- Antibiotic treatment alters the colonic mucus layer and predisposes the host to exacerbated Citrobacter rodentium-induced colitis. (usherbrooke.ca)
- Here, we show that in vivo depletion of CX 3 CR1 + mononuclear phagocytes (MNPs) resulted in more severe colitis and death after infection with Citrobacter rodentium . (rupress.org)
Escherichia9
- Citrobacter rodentium is a mucosal pathogen of mice that shares several pathogenic mechanisms with enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC), which are two clinically important human gastrointestinal pathogens. (nih.gov)
- Citrobacter rodentium is a classically noninvasive pathogen of mice that is similar to enteropathogenic Escherichia coli (EPEC) in man. (asm.org)
- Citrobacter rodentium , a natural mouse pathogen, belongs to the family of extracellular enteric pathogens that includes enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC). (asm.org)
- Here, upon infection with Citrobacter rodentium , a model for enteropathogenic Escherichia coli infection in humans, induced the infiltration of a large number of S100A4 + cells into the colon in wild type (WT) mice. (medicalrecords.com)
- Citrobacter rodentium is used as an in vivo model system for clinically significant enteric pathogens such as enterohaemorrhagic Escherichia coli (EHEC) and enteropathogenic E. coli (EPEC). (ox.ac.uk)
- Diarrheagenic enterohemorrhagic Escherichia coli (EHEC), enteropathogenic E. coli (EPEC), and Citrobacter rodentium (CR) are attaching/effacing (A/E) bacterial pathogens that attach to host intestinal epithelium and efface brush border microvilli, forming A/E lesions ( 1 , 2 ). (pnas.org)
- Intestinal epithelial barrier damage was assessed in Caco-2 cells incubated with three important enteropathogens identified in EE patients: Enteropathogenic Escherichia coli (EPEC), Citrobacter rodentium ( C. rodentium ), and Cryptosporidium parvum ( C. parvum ). (springer.com)
- RT "The Citrobacter rodentium genome sequence reveals convergent RT evolution with human pathogenic Escherichia coli. (genome.jp)
- Modulation of host cytoskeleton function by the enteropathogenic Escherichia coli and Citrobacter rodentium effector protein EspG. (k-state.edu)
Hyperplasia10
- Here we review the history, clinical significance, pathology and molecular pathogenesis of Citrobacter rodentium, the causative agent of transmissible murine colonic hyperplasia. (nih.gov)
- Since T-cell responses in mouse models of inflammatory bowel disease (IBD) also cause epithelial hyperplasia, we have investigated the possibility that C. rodentium promotes similar T-cell responses in the mucosa, thereby increasing epithelial shedding, transmission, and replication of the organism. (asm.org)
- Citrobacter rodentium , formerly Citrobacter freundii biotype 4280, causes transmissible murine colonic hyperplasia ( 6 , 45 , 46 ). (asm.org)
- Of the main hallmarks of C. rodentium infection are colonic crypt hyperplasia (CCH) and dysbiosis. (inserm.fr)
- Fermented dairy products modulate Citrobacter rodentium-induced colonic hyperplasia. (lshtm.ac.uk)
- Weight loss, colonic epithelial cell hyperplasia, mucosal barrier dysfunction, and elevated serum corticosterone levels in C. rodentium-infected wild-type mice were ameliorated by probiotics, but not in ragl -/- animals. (elsevier.com)
- Furthermore, colonic epithelial cell hyperplasia, the hallmark of C. rodentium infection, was reduced in OPN -/- mice, and spleen enlargement by infection was absent in OPN -/- mice. (elsevier.com)
- Sherman, Philip M. / Osteopontin mediates Citrobacter rodentium-induced colonic epithelial cell hyperplasia and attaching-effacing lesions . (elsevier.com)
- Moreover, colonic hyperplasia and diarrhea, which are features of infections with C. rodentium, were not observed in mice infected with the pstCA mutant but did occur in mice given the trans-complemented mutant. (monash.edu)
- Citrobacter rodentium (CR) promotes crypt hyperplasia and tumorigenesis by aberrantly regulating Wnt/β-catenin signaling. (sigmaaldrich.com)
Pathogenesis2
- Citrobacter rodentium is a murine enteropathogen which infects mice using the same 'modus operandi' as EPEC and EHEC, allowing many aspects of pathogenesis to be explored in vivo. (auckland.ac.nz)
- Infection of mice with Citrobacter rodentium is a robust model to study bacterial pathogenesis, mucosal immunology, the health benefits of probiotics and the role of the microbiota during infection. (lshtm.ac.uk)
EHEC10
- Thus, C. rodentium has long been used as a model to understand the molecular basis of EPEC and EHEC infection in vivo. (nih.gov)
- C. rodentium amongst mice populations has been used by many researchers to represent intestinal E. coli since intestinal EPEC or EHEC does not infect mice. (hindawi.com)
- The work I present in this thesis lays the foundations for future studies of the role of EVs in vivo, using infection of mice by C. rodentium as a convenient model system for EPEC and EHEC. (auckland.ac.nz)
- As a result, C. rodentium has become a popular surrogate model for in vivo studies, providing the ability to manipulate both the pathogen ( 7 ) and the host ( 27 ), and interesting insights have been gained into the in vivo roles of many gene products that are common to C. rodentium , EPEC, and EHEC. (asm.org)
- Both EPEC and EHEC are poorly pathogenic in mice, but C . rodentium is a Gram-negative A/E bacterium that specifically infects the mouse colon epithelial cells and causes damage to the epithelial layer 10 , 11 . (medicalrecords.com)
- The group observed a similar pattern when mice were infected with their equivalent of EHEC, the gut bacterium Citrobacter rodentium. (utsouthwestern.edu)
- C. rodentium is used in mice to mimic the effects EHEC produces in humans. (liberty.edu)
- Enteropathogenic E. coli (EPEC), Enterohaemorhagic E. coli (EHEC) and Citrobacter rodentium are constituent members of the attaching and effacing (A/E) pathogens. (bl.uk)
- Citrobacter rodentium is a murine pathogen which is an excellent animal model for EPEC and EHEC infection. (bl.uk)
- EPEC, EHEC and C. rodentium conserve a genomic region termed the locus of enterocyte effacement (LEE) which encodes a type 3 secretion system (T3SS), a core set of type 3 secreted effector proteins and the outer membrane adhesin intimin, which are essential for A/E lesion formation. (bl.uk)
Inflammation4
- and these changes led to better control of inflammation and resolution of infection with C. rodentium . (hindawi.com)
- Surprisingly, susceptibility due to loss of Irgm1 function was not linked to defective intracellular killing of C. rodentium or exaggerated inflammation, but was instead linked to failure to remodel specific colon lamina propria (C-LP) myeloid cells that expand in response to C. rodentium infection and are essential for C. rodentium immunity. (doaj.org)
- Apoptotic susceptibility was induced by C. rodentium infection and was specific to this setting of pathogen infection, and was not apparent in other settings of intestinal inflammation. (doaj.org)
- Research in the Shi lab has shown that helminth co-infection results in an impaired host protection and the development of more severe C. rodentium -mediated intestinal inflammation by a STAT 6 (Th2) dependent mechanism. (massgeneral.org)
Complete Genome Sequence of Citrobacter1
- Complete Genome Sequence of Citrobacter rodentium Strain DBS100. (nih.gov)
C57BL5
- C57BL/6 mice were tested in order to investigate the effects of dietary chitosan (COS) supplements on intestinal microflora and resistance to Citrobacter rodentium infection. (hindawi.com)
- NleH expression was detrimental to C. rodentium in C57BL/10ScNJ mice, which do not mount LPS-induced inflammatory responses. (k-state.edu)
- Infection of C57BL/6 mice with C. rodentium was performed at postnatal day 14. (elsevier.com)
- Here, we show that miR-155-deficient mice are less able to eradicate a mucosal Citrobacter rodentium infection than wild-type C57BL/6 mice. (elsevier.com)
- C57BL/6 mice infected perorally with the pstCA mutant of C. rodentium excreted significantly lower numbers of C. rodentium than those given the wild-type strain. (monash.edu)
Susceptibility2
- Here we find that loss of Irgm1 confers marked susceptibility to Citrobacter rodentium, a noninvasive intestinal pathogen that models inflammatory responses to intestinal bacteria. (doaj.org)
- Active vitamin D (1,25-dihydroxyvitamin D3) increases host susceptibility to Citrobacter rodentium by suppressing mucosal Th17 responses. (greenmedinfo.com)
Type III secretion s1
- In particular, the translocated type III secretion system effector proteins map , espF , and tir have recently been implicated in diarrhea using the C. rodentium mouse model ( 8 , 18 , 28 ). (asm.org)
Microbiota3
- When infected, 1-3% of the mice's intestinal microbiota becomes C. rodentium [ 7 ], whilst the colon comes under attack by 10 9 colony forming units (CFUs) per g [ 8 ]. (hindawi.com)
- Vulnerability to contracting C. rodentium , as well as tendency to show a certain immune response, has been found to be highly related to intestinal microbiota composition [ 10 ]. (hindawi.com)
- In order to colonize the host and compete with the gut microbiota, C. rodentium employs a type III secretion system (T3SS) that injects effectors into colonic intestinal epithelial cells (IECs). (inserm.fr)
Enteric2
- In conclusion, gastrointestinal infection with C. rodentium alters systemic and hepatocytes specific responses, not previously appreciated from this enteric pathogen, making it a useful model for studying host-pathogen interactions under acute hepatic inflammatory stress and injury. (mit.edu)
- This system involves two murine enteric infectious agents that induce distinct Th responses: (i) the helminth Heligmosomoides polygyrus (Th2) and (ii) the Gram-negative bacterium Citrobacter rodentium (Th1). (massgeneral.org)
Gastrointestinal2
- However, by day 21 post-oral challenge, C. rodentium is cleared from the gastrointestinal tracts of normal mice ( 31 ). (asm.org)
- To better understand how lung and gastrointestinal pathogens affect VA status, we assessed VA concentrations in serum, lung, and liver during an invasive pneumonia infection induced by Streptococcus pneumoniae serotype 3, and a noninvasive gut infection induced by Citrobacter rodentium, in vitamin A-adequate (VAA) and vitamin A-deficient (VAD) mice. (elsevier.com)
Salmonella1
- The Salmonella and C. rodentium thrived in this weakened gut environment. (medicalnewstoday.com)
Freundii biotype1
- OS Citrobacter rodentium (strain ICC168) (Citrobacter freundii biotype OS 4280). (genome.jp)
Effector1
- Here we report that the effector EspO ,an orthologue of OspE found in Shigella spp, affects proliferation of IECs 8 and 14 days post C. rodentium infection as well as secretion of IL-22 from colonic explants. (inserm.fr)
Pathogenicity2
- As prior studies have shown EV production by E. coli, this begs the question of whether the same is true for C. rodentium, which would provide a useful model for exploring the role of EVs in pathogenicity. (auckland.ac.nz)
- Pomegranate peel extract reduced the pathogenicity of Citrobacter rodentium-infections in mice. (usda.gov)
Coli5
- C. rodentium that expressed both E. coli NleH1 and NleH2 was hypervirulent in C3H/HeJ mice. (k-state.edu)
- pathogens with the closely related human pathogen, en- teropathogenic E. coli ( 4 , 5 ). (cdc.gov)
- In the experiment, mice were orally infected with a bacterium (Citrobacter rodentium) that mimics infection by E. coli in humans. (eurekalert.org)
- They infected the mice with Citrobacter rodentium, a species of bacteria that is the rodent equivalent of E. coli, which infects humans. (eurekalert.org)
- Next, the investigators tried the same experiment using Citrobacter rodentium - a strain of bacteria similar to the E. coli strains that affect humans. (medicalnewstoday.com)
Responses4
- Infection with Citrobacter rodentium triggers robust tissue damage repair responses, manifested by secretion of IL-22, in the absence of which mice succumbed to the infection. (inserm.fr)
- These results suggest that C. rodentium manipulates host metabolism to evade innate immune responses and establish a favorable gut ecosystem. (cf.ac.uk)
- Germinal center formation and humoral immune responses against C. rodentium were severely impaired in infected miR-155-deficient mice. (elsevier.com)
- Gamma interferon produced by antigen-specific CD4+ T cells regulates the mucosal immune responses to Citrobacter rodentium infection. (helmholtz-hzi.de)
Strain1
- Though the Citrobacter rodentium ( C. rodentium ) strain DBS100 has been studied extensively, it had not previously been sequenced. (liberty.edu)
Bacteria2
- Citrobacter rodentium is a Gram-negative species of bacteria. (wikipedia.org)
- C. rodentium shows a remarkable ability to colonize the murine colon, with over 10 9 bacteria present during the peak of infection. (asm.org)
Severity2
- Therefore, the aims of this study were to characterize Citrobacter rodentium infection in neonatal mice and determine the role played by specific probiotics in ameliorating disease severity. (elsevier.com)
- Probiotic Lactobacillus reuteri attenuates the stressor-enhanced severity of Citrobacter rodentium infection. (greenmedinfo.com)
Noninvasive1
- These data also demonstrate that non-NF-κB-dependent defenses are insufficient to control C. rodentium infection, and hence, the NF-κB p50 subunit is critical for defense against this noninvasive pathogen. (asm.org)
Systemic1
- Irgm1-deficient mice fail to control C. rodentium outgrowth in the intestine, leading to systemic pathogen spread and host mortality. (doaj.org)
Murine model1
- However, since Moxley and Francis's review in 1998 ( 2 ), several advances have been made in the field, including the generation of a Shiga toxin (Stx)-producing Citrobacter rodentium -murine model, a human intestine xenograft murine model, and a renewed interest in the use of rabbit models. (asmscience.org)
Intestinal epithelial cells1
- In vitro , soluble S100A4 directly up-regulated expression of integrin β−1 in intestinal epithelial cells and significantly increased the adherence of C . rodentium to intestinal epithelial cells. (medicalrecords.com)
Werkmanii1
- In our previous study, Citrobacter werkmanii BF-6 was isolated from an industrial spoilage sample and demonstrated an excellent ability to form biofilms, which could be affected by various environmental factors. (biomedcentral.com)
Epithelial cell2
- In vivo, C. rodentium infection is characterized by increased epithelial cell proliferation, mucosal thickening, and a TH1-type immune response, but with protective immunity believed to be mediated by serum immunoglobulin G (IgG). (asm.org)
- C. rodentium was administered to both control and intestinal epithelial cell ILK knockout mice. (biomedcentral.com)
Wild-type mice3
- OPN gene knockout and wild-type mice were infected orogastrically with either C. rodentium or Luria-Bertani (LB) broth. (elsevier.com)
- Colonic expression of OPN was increased by C. rodentium infection of wild-type mice. (elsevier.com)
- Compared with wild-type mice, Nlrp6 -/- mice inoculated with Citrobacter rodentium had more persistent and severe colonic infections. (sciencemag.org)
Vivo1
- C. rodentium exposure was shown to increase ILK expression in cell lines, and in murine epithelium in vivo . (biomedcentral.com)
Mouse5
- C. rodentium serves as an important model pathogen for investigating the mechanisms controlling attaching and effacing pathology, epithelial hyperproliferation, and tumor promotion in the distal colon of the mouse. (nih.gov)
- Citrobacter rodentium mouse model of bacterial infection. (lshtm.ac.uk)
- C. rodentium was first isolated by Barthold from an outbreak of mouse diarrhea in Yale University in 1972 and was 'rediscovered' by Falkow and Schauer in 1993. (lshtm.ac.uk)
- We thank Rosanna Mundy (Imperial College, London, United Kingdom) for constructing the STM library of C. rodentium, Judyta Praszkier (The University of Melbourne) for the gift of plasmids used in this study, Sau Fung Lee (University of Melbourne) for FAS data, and Danijela Krmek for assisting with mouse experiments.This work was supported by grants from the Australian National Health and Medical Research Council and the Australian Research Council. (edu.au)
- Mouse-derived OPN +/+ and OPN -/- fibroblasts were incubated with C. rodentium and attachingeffacing lesions were demonstrated using transmission electron microscopy and immunofluorescence. (elsevier.com)
Phenotype1
- To investigate this, we inactivated phoB in the pstCA mutants of EPEC E128012 and C. rodentium and found that the phoB mutation restored the adherent phenotype of both mutant strains. (monash.edu)
Mice infected1
- Moreover, mice infected with ΔespO triggered significantly lesser nutritional immunity (e.g. calprotectin, Lcn2) and expression of antimicrobial peptides (Reg3β, Reg3γ) compared to mice infected with WT C. rodentium. (inserm.fr)
Dysbiosis1
- Pomegranate peel extract alters the microbiome in mice and dysbiosis caused by Citrobacter rodentium infection. (usda.gov)
Tissue damage1
- Dietary oils modify the host immune response and colonic tissue damage following Citrobacter rodentium infection in mice. (greenmedinfo.com)
Host1
- The NF-κB transcription factor c-Rel controls host defense against Citrobacter rodentium. (helmholtz-hzi.de)