A change from planar to elliptic polarization when an initially plane-polarized light wave traverses an optically active medium. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The level of protein structure in which regular hydrogen-bond interactions within contiguous stretches of polypeptide chain give rise to alpha helices, beta strands (which align to form beta sheets) or other types of coils. This is the first folding level of protein conformation.
Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)
The characteristic 3-dimensional shape of a protein, including the secondary, supersecondary (motifs), tertiary (domains) and quaternary structure of the peptide chain. PROTEIN STRUCTURE, QUATERNARY describes the conformation assumed by multimeric proteins (aggregates of more than one polypeptide chain).
Determination of the spectra of ultraviolet absorption by specific molecules in gases or liquids, for example Cl2, SO2, NO2, CS2, ozone, mercury vapor, and various unsaturated compounds. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Disruption of the non-covalent bonds and/or disulfide bonds responsible for maintaining the three-dimensional shape and activity of the native protein.
Measurement of the intensity and quality of fluorescence.
Processes involved in the formation of TERTIARY PROTEIN STRUCTURE.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A strong organic base existing primarily as guanidium ions at physiological pH. It is found in the urine as a normal product of protein metabolism. It is also used in laboratory research as a protein denaturant. (From Martindale, the Extra Pharmacopoeia, 30th ed and Merck Index, 12th ed) It is also used in the treatment of myasthenia and as a fluorescent probe in HPLC.
The method of measuring the dispersion of an optically active molecule to determine the relative magnitude of right- or left-handed components and sometimes structural features of the molecule.
A rigorously mathematical analysis of energy relationships (heat, work, temperature, and equilibrium). It describes systems whose states are determined by thermal parameters, such as temperature, in addition to mechanical and electromagnetic parameters. (From Hawley's Condensed Chemical Dictionary, 12th ed)
Models used experimentally or theoretically to study molecular shape, electronic properties, or interactions; includes analogous molecules, computer-generated graphics, and mechanical structures.
Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).
A non-aqueous co-solvent that serves as tool to study protein folding. It is also used in various pharmaceutical, chemical and engineering applications.
The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.
The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.
The process in which substances, either endogenous or exogenous, bind to proteins, peptides, enzymes, protein precursors, or allied compounds. Specific protein-binding measures are often used as assays in diagnostic assessments.
Differential thermal analysis in which the sample compartment of the apparatus is a differential calorimeter, allowing an exact measure of the heat of transition independent of the specific heat, thermal conductivity, and other variables of the sample.
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
The rate dynamics in chemical or physical systems.
The parts of a macromolecule that directly participate in its specific combination with another molecule.
An essential amino acid that is necessary for normal growth in infants and for NITROGEN balance in adults. It is a precursor of INDOLE ALKALOIDS in plants. It is a precursor of SEROTONIN (hence its use as an antidepressant and sleep aid). It can be a precursor to NIACIN, albeit inefficiently, in mammals.
The art or process of comparing photometrically the relative intensities of the light in different parts of the spectrum.
The level of protein structure in which combinations of secondary protein structures (alpha helices, beta sheets, loop regions, and motifs) pack together to form folded shapes called domains. Disulfide bridges between cysteines in two different parts of the polypeptide chain along with other interactions between the chains play a role in the formation and stabilization of tertiary structure. Small proteins usually consist of only one domain but larger proteins may contain a number of domains connected by segments of polypeptide chain which lack regular secondary structure.
A group of 13 or more deoxyribonucleotides in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
Members of the class of compounds composed of AMINO ACIDS joined together by peptide bonds between adjacent amino acids into linear, branched or cyclical structures. OLIGOPEPTIDES are composed of approximately 2-12 amino acids. Polypeptides are composed of approximately 13 or more amino acids. PROTEINS are linear polypeptides that are normally synthesized on RIBOSOMES.
A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.
Proteins prepared by recombinant DNA technology.
A class of organic compounds which contain an anilino (phenylamino) group linked to a salt or ester of naphthalenesulfonic acid. They are frequently used as fluorescent dyes and sulfhydryl reagents.
Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.
The relationship between the chemical structure of a compound and its biological or pharmacological activity. Compounds are often classed together because they have structural characteristics in common including shape, size, stereochemical arrangement, and distribution of functional groups.
Spectrophotometry in the infrared region, usually for the purpose of chemical analysis through measurement of absorption spectra associated with rotational and vibrational energy levels of molecules. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.
The characteristic three-dimensional shape of a molecule.
Partial proteins formed by partial hydrolysis of complete proteins or generated through PROTEIN ENGINEERING techniques.
The homogeneous mixtures formed by the mixing of a solid, liquid, or gaseous substance (solute) with a liquid (the solvent), from which the dissolved substances can be recovered by physical processes. (From Grant & Hackh's Chemical Dictionary, 5th ed)
Presence of warmth or heat or a temperature notably higher than an accustomed norm.
The extent to which an enzyme retains its structural conformation or its activity when subjected to storage, isolation, and purification or various other physical or chemical manipulations, including proteolytic enzymes and heat.
Higher-order DNA and RNA structures formed from guanine-rich sequences. They are formed around a core of at least 2 stacked tetrads of hydrogen-bonded GUANINE bases. They can be formed from one two or four separate strands of DNA (or RNA) and can display a wide variety of topologies, which are a consequence of various combinations of strand direction, length, and sequence. (From Nucleic Acids Res. 2006;34(19):5402-15)
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).
The diversion of RADIATION (thermal, electromagnetic, or nuclear) from its original path as a result of interactions or collisions with atoms, molecules, or larger particles in the atmosphere or other media. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A compound formed in the liver from ammonia produced by the deamination of amino acids. It is the principal end product of protein catabolism and constitutes about one half of the total urinary solids.
The ability of a protein to retain its structural conformation or its activity when subjected to physical or chemical manipulations.
The phenomenon whereby compounds whose molecules have the same number and kind of atoms and the same atomic arrangement, but differ in their spatial relationships. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 5th ed)
Centrifugation with a centrifuge that develops centrifugal fields of more than 100,000 times gravity. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Domesticated bovine animals of the genus Bos, usually kept on a farm or ranch and used for the production of meat or dairy products or for heavy labor.
The location of the atoms, groups or ions relative to one another in a molecule, as well as the number, type and location of covalent bonds.
A family of iminourea derivatives. The parent compound has been isolated from mushrooms, corn germ, rice hulls, mussels, earthworms, and turnip juice. Derivatives may have antiviral and antifungal properties.
The measurement of the quantity of heat involved in various processes, such as chemical reactions, changes of state, and formations of solutions, or in the determination of the heat capacities of substances. The fundamental unit of measurement is the joule or the calorie (4.184 joules). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The protein components of a number of complexes, such as enzymes (APOENZYMES), ferritin (APOFERRITINS), or lipoproteins (APOLIPOPROTEINS).
Electrophoresis in which a polyacrylamide gel is used as the diffusion medium.
Conformational transitions of the shape of a protein to various unfolded states.
Particles consisting of aggregates of molecules held loosely together by secondary bonds. The surface of micelles are usually comprised of amphiphatic compounds that are oriented in a way that minimizes the energy of interaction between the micelle and its environment. Liquids that contain large numbers of suspended micelles are referred to as EMULSIONS.
A left-handed double helix of DNA. Its name derives from its narrow zigzag structure that is the least twisted and thinnest form of DNA. Z-DNA forming regions within the GENOME may play an important role in GENE EXPRESSION REGULATION.
Genetically engineered MUTAGENESIS at a specific site in the DNA molecule that introduces a base substitution, or an insertion or deletion.
Chromatography on non-ionic gels without regard to the mechanism of solute discrimination.
The process by which two molecules of the same chemical composition form a condensation product or polymer.
Theoretical representations that simulate the behavior or activity of chemical processes or phenomena; includes the use of mathematical equations, computers, and other electronic equipment.
A trypanocidal agent and possible antiviral agent that is widely used in experimental cell biology and biochemistry. Ethidium has several experimentally useful properties including binding to nucleic acids, noncompetitive inhibition of nicotinic acetylcholine receptors, and fluorescence among others. It is most commonly used as the bromide.
The reconstitution of a protein's activity following denaturation.
Analysis of the intensity of Raman scattering of monochromatic light as a function of frequency of the scattered light.
Devices for accelerating protons or electrons in closed orbits where the accelerating voltage and magnetic field strength varies (the accelerating voltage is held constant for electrons) in order to keep the orbit radius constant.
Chemical groups containing the covalent disulfide bonds -S-S-. The sulfur atoms can be bound to inorganic or organic moieties.
The study of MAGNETIC PHENOMENA.
Proteins found in any species of bacterium.
The chemical and physical integrity of a pharmaceutical product.
The thermodynamic interaction between a substance and WATER.
Compounds and molecular complexes that consist of very large numbers of atoms and are generally over 500 kDa in size. In biological systems macromolecular substances usually can be visualized using ELECTRON MICROSCOPY and are distinguished from ORGANELLES by the lack of a membrane structure.
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
Liquids that dissolve other substances (solutes), generally solids, without any change in chemical composition, as, water containing sugar. (Grant & Hackh's Chemical Dictionary, 5th ed)
The characteristic 3-dimensional shape and arrangement of multimeric proteins (aggregates of more than one polypeptide chain).
Agents that are capable of inserting themselves between the successive bases in DNA, thus kinking, uncoiling or otherwise deforming it and therefore preventing its proper functioning. They are used in the study of DNA.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
A conjugated protein which is the oxygen-transporting pigment of muscle. It is made up of one globin polypeptide chain and one heme group.
The property of emitting radiation while being irradiated. The radiation emitted is usually of longer wavelength than that incident or absorbed, e.g., a substance can be irradiated with invisible radiation and emit visible light. X-ray fluorescence is used in diagnosis.
An anionic surfactant, usually a mixture of sodium alkyl sulfates, mainly the lauryl; lowers surface tension of aqueous solutions; used as fat emulsifier, wetting agent, detergent in cosmetics, pharmaceuticals and toothpastes; also as research tool in protein biochemistry.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
Organic compounds that generally contain an amino (-NH2) and a carboxyl (-COOH) group. Twenty alpha-amino acids are the subunits which are polymerized to form proteins.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
The determination of the concentration of a given component in solution (the analyte) by addition of a liquid reagent of known strength (the titrant) until an equivalence point is reached (when the reactants are present in stoichiometric proportions). Often an indicator is added to make the equivalence point visible (e.g., a change in color).
A group of 13 or more ribonucleotides in which the phosphate residues of each ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
Globulins of milk obtained from the WHEY.
A major protein fraction of milk obtained from the WHEY.
Layers of lipid molecules which are two molecules thick. Bilayer systems are frequently studied as models of biological membranes.
The rotation of linearly polarized light as it passes through various media.
Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.
The study of PHYSICAL PHENOMENA and PHYSICAL PROCESSES as applied to living things.
The sum of the weight of all the atoms in a molecule.
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
Conformational transitions of a protein from unfolded states to a more folded state.
Polymers made up of a few (2-20) nucleotides. In molecular genetics, they refer to a short sequence synthesized to match a region where a mutation is known to occur, and then used as a probe (OLIGONUCLEOTIDE PROBES). (Dorland, 28th ed)
A basic science concerned with the composition, structure, and properties of matter; and the reactions that occur between substances and the associated energy exchange.
The concentration of osmotically active particles in solution expressed in terms of osmoles of solute per liter of solution. Osmolality is expressed in terms of osmoles of solute per kilogram of solvent.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The assembly of the QUATERNARY PROTEIN STRUCTURE of multimeric proteins (MULTIPROTEIN COMPLEXES) from their composite PROTEIN SUBUNITS.
The temperature at which a substance changes from one state or conformation of matter to another.
Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.
The physical characteristics and processes of biological systems.
A clear, odorless, tasteless liquid that is essential for most animal and plant life and is an excellent solvent for many substances. The chemical formula is hydrogen oxide (H2O). (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A low-energy attractive force between hydrogen and another element. It plays a major role in determining the properties of water, proteins, and other compounds.
The composition, conformation, and properties of atoms and molecules, and their reaction and interaction processes.
A synthetic phospholipid used in liposomes and lipid bilayers for the study of biological membranes.
The scattering of x-rays by matter, especially crystals, with accompanying variation in intensity due to interference effects. Analysis of the crystal structure of materials is performed by passing x-rays through them and registering the diffraction image of the rays (CRYSTALLOGRAPHY, X-RAY). (From McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
A molecule that binds to another molecule, used especially to refer to a small molecule that binds specifically to a larger molecule, e.g., an antigen binding to an antibody, a hormone or neurotransmitter binding to a receptor, or a substrate or allosteric effector binding to an enzyme. Ligands are also molecules that donate or accept a pair of electrons to form a coordinate covalent bond with the central metal atom of a coordination complex. (From Dorland, 27th ed)
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Basic polypeptide from the venom of the honey bee (Apis mellifera). It contains 26 amino acids, has cytolytic properties, causes contracture of muscle, releases histamine, and disrupts surface tension, probably due to lysis of cell and mitochondrial membranes.
Measurement of the polarization of fluorescent light from solutions or microscopic specimens. It is used to provide information concerning molecular size, shape, and conformation, molecular anisotropy, electronic energy transfer, molecular interaction, including dye and coenzyme binding, and the antigen-antibody reaction.
Procedures by which protein structure and function are changed or created in vitro by altering existing or synthesizing new structural genes that direct the synthesis of proteins with sought-after properties. Such procedures may include the design of MOLECULAR MODELS of proteins using COMPUTER GRAPHICS or other molecular modeling techniques; site-specific mutagenesis (MUTAGENESIS, SITE-SPECIFIC) of existing genes; and DIRECTED MOLECULAR EVOLUTION techniques to create new genes.
Liquid chromatographic techniques which feature high inlet pressures, high sensitivity, and high speed.
Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.
An isoform of DNA that occurs in an environment rich in SODIUM and POTASSIUM ions. It is a right-handed helix with 11 base pairs per turn, a pitch of 0.256 nm per base pair and a helical diameter of 2.3 nm.
An essential amino acid that is required for the production of HISTAMINE.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
Polydeoxyribonucleotides made up of deoxyadenine nucleotides and thymine nucleotides. Present in DNA preparations isolated from crab species. Synthetic preparations have been used extensively in the study of DNA.
Substances produced from the reaction between acids and bases; compounds consisting of a metal (positive) and nonmetal (negative) radical. (Grant & Hackh's Chemical Dictionary, 5th ed)
Common name for the species Gallus gallus, the domestic fowl, in the family Phasianidae, order GALLIFORMES. It is descended from the red jungle fowl of SOUTHEAST ASIA.
A group of deoxyribonucleotides (up to 12) in which the phosphate residues of each deoxyribonucleotide act as bridges in forming diester linkages between the deoxyribose moieties.
The naturally occurring or experimentally induced replacement of one or more AMINO ACIDS in a protein with another. If a functionally equivalent amino acid is substituted, the protein may retain wild-type activity. Substitution may also diminish, enhance, or eliminate protein function. Experimentally induced substitution is often used to study enzyme activities and binding site properties.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.
The ability of a substance to be dissolved, i.e. to form a solution with another substance. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A fibrous protein complex that consists of proteins folded into a specific cross beta-pleated sheet structure. This fibrillar structure has been found as an alternative folding pattern for a variety of functional proteins. Deposits of amyloid in the form of AMYLOID PLAQUES are associated with a variety of degenerative diseases. The amyloid structure has also been found in a number of functional proteins that are unrelated to disease.
The study of crystal structure using X-RAY DIFFRACTION techniques. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.
A serine endopeptidase that is formed from TRYPSINOGEN in the pancreas. It is converted into its active form by ENTEROPEPTIDASE in the small intestine. It catalyzes hydrolysis of the carboxyl group of either arginine or lysine. EC 3.4.21.4.
A thiol-containing non-essential amino acid that is oxidized to form CYSTINE.
Large, hoofed mammals of the family EQUIDAE. Horses are active day and night with most of the day spent seeking and consuming food. Feeding peaks occur in the early morning and late afternoon, and there are several daily periods of rest.
A metallic element of atomic number 30 and atomic weight 65.38. It is a necessary trace element in the diet, forming an essential part of many enzymes, and playing an important role in protein synthesis and in cell division. Zinc deficiency is associated with ANEMIA, short stature, HYPOGONADISM, impaired WOUND HEALING, and geophagia. It is known by the symbol Zn.
A biosensing technique in which biomolecules capable of binding to specific analytes or ligands are first immobilized on one side of a metallic film. Light is then focused on the opposite side of the film to excite the surface plasmons, that is, the oscillations of free electrons propagating along the film's surface. The refractive index of light reflecting off this surface is measured. When the immobilized biomolecules are bound by their ligands, an alteration in surface plasmons on the opposite side of the film is created which is directly proportional to the change in bound, or adsorbed, mass. Binding is measured by changes in the refractive index. The technique is used to study biomolecular interactions, such as antigen-antibody binding.
A basic enzyme that is present in saliva, tears, egg white, and many animal fluids. It functions as an antibacterial agent. The enzyme catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. EC 3.2.1.17.
The study of CHEMICAL PHENOMENA and processes in terms of the underlying PHYSICAL PHENOMENA and processes.
A type of scanning probe microscopy in which a probe systematically rides across the surface of a sample being scanned in a raster pattern. The vertical position is recorded as a spring attached to the probe rises and falls in response to peaks and valleys on the surface. These deflections produce a topographic map of the sample.
A major protein in the BLOOD. It is important in maintaining the colloidal osmotic pressure and transporting large organic molecules.
A serine endopeptidase secreted by the pancreas as its zymogen, CHYMOTRYPSINOGEN and carried in the pancreatic juice to the duodenum where it is activated by TRYPSIN. It selectively cleaves aromatic amino acids on the carboxyl side.
Linear POLYPEPTIDES that are synthesized on RIBOSOMES and may be further modified, crosslinked, cleaved, or assembled into complex proteins with several subunits. The specific sequence of AMINO ACIDS determines the shape the polypeptide will take, during PROTEIN FOLDING, and the function of the protein.
A brominating agent that replaces hydrogen atoms in benzylic or allylic positions. It is used in the oxidation of secondary alcohols to ketones and in controlled low-energy brominations. (From Miall's Dictionary of Chemistry, 5th ed; Hawley's Condensed Chemical Dictionary, 12th ed,).
A nitrogen-free class of lipids present in animal and particularly plant tissues and composed of one mole of glycerol and 1 or 2 moles of phosphatidic acid. Members of this group differ from one another in the nature of the fatty acids released on hydrolysis.
Derivatives of phosphatidic acids in which the phosphoric acid is bound in ester linkage to a choline moiety. Complete hydrolysis yields 1 mole of glycerol, phosphoric acid and choline and 2 moles of fatty acids.
The physical phenomena describing the structure and properties of atoms and molecules, and their reaction and interaction processes.
A group of compounds containing the porphin structure, four pyrrole rings connected by methine bridges in a cyclic configuration to which a variety of side chains are attached. The nature of the side chain is indicated by a prefix, as uroporphyrin, hematoporphyrin, etc. The porphyrins, in combination with iron, form the heme component in biologically significant compounds such as hemoglobin and myoglobin.
Toxins isolated from the venom of Laticauda semifasciata, a sea snake (Hydrophid); immunogenic, basic polypeptides of 62 amino acids, folded by four disulfide bonds, block neuromuscular end-plates irreversibly, thus causing paralysis and severe muscle damage; they are similar to Elapid neurotoxins.
A group of peptide antibiotics from BACILLUS brevis. Gramicidin C or S is a cyclic, ten-amino acid polypeptide and gramicidins A, B, D are linear. Gramicidin is one of the two principal components of TYROTHRICIN.
A trace element that is a component of vitamin B12. It has the atomic symbol Co, atomic number 27, and atomic weight 58.93. It is used in nuclear weapons, alloys, and pigments. Deficiency in animals leads to anemia; its excess in humans can lead to erythrocytosis.
The facilitation of a chemical reaction by material (catalyst) that is not consumed by the reaction.
Purifying or cleansing agents, usually salts of long-chain aliphatic bases or acids, that exert cleansing (oil-dissolving) and antimicrobial effects through a surface action that depends on possessing both hydrophilic and hydrophobic properties.
A group of guanine ribonucleotides in which the phosphate residues of each guanine ribonucleotide act as bridges in forming diester linkages between the ribose moieties.
Proteins produced from GENES that have acquired MUTATIONS.
A ubiquitous sodium salt that is commonly used to season food.
The transfer of energy of a given form among different scales of motion. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed). It includes the transfer of kinetic energy and the transfer of chemical energy. The transfer of chemical energy from one molecule to another depends on proximity of molecules so it is often used as in techniques to measure distance such as the use of FORSTER RESONANCE ENERGY TRANSFER.
Inorganic or organic compounds that contain divalent iron.
Small cationic peptides that are an important component, in most species, of early innate and induced defenses against invading microbes. In animals they are found on mucosal surfaces, within phagocytic granules, and on the surface of the body. They are also found in insects and plants. Among others, this group includes the DEFENSINS, protegrins, tachyplesins, and thionins. They displace DIVALENT CATIONS from phosphate groups of MEMBRANE LIPIDS leading to disruption of the membrane.
The accumulation of an electric charge on a object
A non-crystalline form of silicon oxide that has absorptive properties. It is commonly used as a desiccating agent and as a stationary phase for CHROMATOGRAPHY. The fully hydrated form of silica gel has distinct properties and is referred to as SILICIC ACID.
A representation, generally small in scale, to show the structure, construction, or appearance of something. (From Random House Unabridged Dictionary, 2d ed)
Proteins that have one or more tightly bound metal ions forming part of their structure. (Dorland, 28th ed)
Commonly observed structural components of proteins formed by simple combinations of adjacent secondary structures. A commonly observed structure may be composed of a CONSERVED SEQUENCE which can be represented by a CONSENSUS SEQUENCE.
Single membrane vesicles, generally made of PHOSPHOLIPIDS.
An acid dye used in testing for hydrochloric acid in gastric contents. It is also used histologically to test for AMYLOIDOSIS.
A heavy metal trace element with the atomic symbol Cu, atomic number 29, and atomic weight 63.55.
Peptides composed of between two and twelve amino acids.
Pyrrole containing pigments found in photosynthetic bacteria.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
The deductive study of shape, quantity, and dependence. (From McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
A non-essential amino acid that occurs in high levels in its free state in plasma. It is produced from pyruvate by transamination. It is involved in sugar and acid metabolism, increases IMMUNITY, and provides energy for muscle tissue, BRAIN, and the CENTRAL NERVOUS SYSTEM.
An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1.
Artificially produced membranes, such as semipermeable membranes used in artificial kidney dialysis (RENAL DIALYSIS), monomolecular and bimolecular membranes used as models to simulate biological CELL MEMBRANES. These membranes are also used in the process of GUIDED TISSUE REGENERATION.
An essential amino acid. It is often added to animal feed.
Positively charged atoms, radicals or groups of atoms which travel to the cathode or negative pole during electrolysis.
A colorless, odorless, highly water soluble vinyl monomer formed from the hydration of acrylonitrile. It is primarily used in research laboratories for electrophoresis, chromatography, and electron microscopy and in the sewage and wastewater treatment industries.
A chemical system that functions to control the levels of specific ions in solution. When the level of hydrogen ion in solution is controlled the system is called a pH buffer.
Polymers synthesized by living organisms. They play a role in the formation of macromolecular structures and are synthesized via the covalent linkage of biological molecules, especially AMINO ACIDS; NUCLEOTIDES; and CARBOHYDRATES.
A benzimidazole antifilarial agent; it is fluorescent when it binds to certain nucleotides in DNA, thus providing a tool for the study of DNA replication; it also interferes with mitosis.
A basic polypeptide isolated from Streptomyces netropsis. It is cytotoxic and its strong, specific binding to A-T areas of DNA is useful to genetics research.
Proteins conjugated with deoxyribonucleic acids (DNA) or specific DNA.
The process of cleaving a chemical compound by the addition of a molecule of water.
Proteins obtained from species in the class of AMPHIBIANS.

R73A and H144Q mutants of the yeast mitochondrial cyclophilin Cpr3 exhibit a low prolyl isomerase activity in both peptide and protein-folding assays. (1/11120)

Previously we reported that the R73A and H144Q variants of the yeast cyclophilin Cpr3 were virtually inactive in a protease-coupled peptide assay, but retained activity as catalysts of a proline-limited protein folding reaction [Scholz, C. et al. (1997) FEBS Lett. 414, 69-73]. A reinvestigation revealed that in fact these two mutations strongly decrease the prolyl isomerase activity of Cpr3 in both the peptide and the protein-folding assay. The high folding activities found previously originated from a contamination of the recombinant Cpr3 proteins with the Escherichia coli protein SlyD, a prolyl isomerase that co-purifies with His-tagged proteins. SlyD is inactive in the peptide assay, but highly active in the protein-folding assay.  (+info)

Biophysical characterization of the structure of the amino-terminal region of gp41 of HIV-1. Implications on viral fusion mechanism. (2/11120)

A peptide of 51 amino acids corresponding to the NH2-terminal region (5-55) of the glycoprotein gp41 of human immunodeficiency virus type 1 was synthesized to study its conformation and assembly. Nuclear magnetic resonance experiments indicated the sequence NH2-terminal to the leucine zipper-like domain of gp41 was induced into helix in the micellar solution, in agreement with circular dichroism data. Light scattering experiment showed that the peptide molecules self-assembled in water into trimeric structure on average. That the peptide molecules oligomerize in aqueous solution was supported by gel filtration and diffusion coefficient experiments. Molecular dynamics simulation based on the NMR data revealed a flexible region adjacent to the hydrophobic NH2 terminus of gp41. The biological significance of the present findings on the conformational flexibility and the propensity of oligomerization of the peptide may be envisioned by a proposed model for the interaction of gp41 with membranes during fusion process.  (+info)

Reaction specificity of native and nicked 3,4-dihydroxyphenylalanine decarboxylase. (3/11120)

3,4-Dihydroxyphenylalanine (Dopa) decarboxylase is a stereospecific pyridoxal 5'-phosphate (PLP)-dependent alpha-decarboxylase that converts L-aromatic amino acids into their corresponding amines. We now report that reaction of the enzyme with D-5-hydroxytryptophan or D-Dopa results in a time-dependent inactivation and conversion of the PLP coenzyme to pyridoxamine 5'-phosphate and PLP-D-amino acid Pictet-Spengler adducts, which have been identified by high performance liquid chromatography. We also show that the reaction specificity of Dopa decarboxylase toward aromatic amines depends on the experimental conditions. Whereas oxidative deamination occurs under aerobic conditions (Bertoldi, M., Moore, P. S., Maras, B., Dominici, P., and Borri Voltattorni, C. (1996) J. Biol. Chem. 271, 23954-23959; Bertoldi, M., Dominici, P., Moore, P. S., Maras, B., and Borri Voltattorni, C. (1998) Biochemistry 37, 6552-6561), half-transamination and Pictet-Spengler reactions take place under anaerobic conditions. Moreover, we examined the reaction specificity of nicked Dopa decarboxylase, obtained by selective tryptic cleavage of the native enzyme between Lys334 and His335. Although this enzymatic species does not exhibit either decarboxylase or oxidative deamination activities, it retains a large percentage of the native transaminase activity toward D-aromatic amino acids and displays a slow transaminase activity toward aromatic amines. These transamination reactions occur concomitantly with the formation of cyclic coenzyme-substrate adducts. Together with additional data, we thus suggest that native Dopa decarboxylase can exist as an equilibrium among "open," "half-open," and "closed" forms.  (+info)

SNARE interactions are not selective. Implications for membrane fusion specificity. (4/11120)

The SNARE hypothesis proposes that membrane trafficking specificity is mediated by preferential high affinity interactions between particular v (vesicle membrane)- and t (target membrane)-SNARE combinations. The specificity of interactions among a diverse set of SNAREs, however, is unknown. We have tested the SNARE hypothesis by analyzing potential SNARE complexes between five proteins of the vesicle-associated membrane protein (VAMP) family, three members of the synaptosome-associated protein-25 (SNAP-25) family and three members of the syntaxin family. All of the 21 combinations of SNAREs tested formed stable complexes. Sixteen were resistant to SDS denaturation, and most complexes thermally denatured between 70 and 90 degreesC. These results suggest that the specificity of membrane fusion is not encoded by the interactions between SNAREs.  (+info)

The putative bioactive surface of insect-selective scorpion excitatory neurotoxins. (5/11120)

Scorpion neurotoxins of the excitatory group show total specificity for insects and serve as invaluable probes for insect sodium channels. However, despite their significance and potential for application in insect-pest control, the structural basis for their bioactivity is still unknown. We isolated, characterized, and expressed an atypically long excitatory toxin, Bj-xtrIT, whose bioactive features resembled those of classical excitatory toxins, despite only 49% sequence identity. With the objective of clarifying the toxic site of this unique pharmacological group, Bj-xtrIT was employed in a genetic approach using point mutagenesis and biological and structural assays of the mutant products. A primary target for modification was the structurally unique C-terminal region. Sequential deletions of C-terminal residues suggested an inevitable significance of Ile73 and Ile74 for toxicity. Based on the bioactive role of the C-terminal region and a comparison of Bj-xtrIT with a Bj-xtrIT-based model of a classical excitatory toxin, AaHIT, a conserved surface comprising the C terminus is suggested to form the site of recognition with the sodium channel receptor.  (+info)

Properties of non-polymerizable tropomyosin obtained by carboxypeptidase A digestion. (6/11120)

Tropomyosin digested with carboxypeptidase A [EC 3.4.12.2] (CTM) shows a lower viscosity than the undigested protein in solution. From the relation between the viscosity decrease and the amount of amino acids liberated from the carboxyl terminus during this digestion, it is inferred that loss of the tri-peptide-Thr-Ser-Ile from the C-terminus is responsible for the decrease in viscosity. The secondary structure of -TM was not affected by the digestion according to circular dichroic measurements. The viscosity of CTM did not increase in methanol-water mixtures, whereas that of tropomyosin increased markedly. These results indicate that polymerizability was lost upon the removal of a small peptide from the C-terminus without change in the secondary structure. A decrease in the viscosity of tropomyosin solutions was observed on the addition of CTM, indicating that CTM interacts with intact tropomyosin. The dependence of the viscosity decrease on the amount of CTM showed that CTM binds tropomyosin in a one-to-one ratio as a result of end-to-end interaction. Since paracrystals having a 400 A repeated band structure could be grown in the presence of Mg ions at neutral pH, side-by-side interactions in CTM molecules remain intact, even though polymerizability is lost. The disc gel electrophoretic pattern showed that troponin could bind to CTM, but no increase in viscosity due to the complex was observed in solution. That is, the C-terminal part of tropomyosin is not required for the formation of the complex. The amount of CTM bound to F-actin was less than half of that bound to undigested tropomyosin, and could be reduced to one-tenth by a washing procedure. In the presence of troponin, however, the amount recovered to the level of tropomyosin normally bound to F-actin. Therefore, it is concluded that troponin is bound in the middle of the tropomyosin molecule and strengthens the binding of tropomyosin to F-actin.  (+info)

Characterisation of copper-binding to the second sub-domain of the Menkes protein ATPase (MNKr2). (7/11120)

The Menkes ATPase (MNK) has an essential role in the translocation of copper across cellular membranes. In a complementary manner, the intracellular concentration of copper regulates the activity and cellular location of the ATPase through its six homologous amino-terminal domains. The roles of the six amino-terminal domains in the activation and cellular trafficking processes are unknown. Understanding the role of these domains relies on the development of an understanding of their metal-binding properties and structural properties. The second conserved sub-domain of MNK was over-expressed, purified and its copper-binding properties characterised. Reconstitution studies demonstrate that copper binds to MNKr2 as Cu(I) with a stoichiometry of one copper per domain. This is the first direct evidence of copper-binding to the MNK amino-terminal repeats. Circular dichroism studies suggest that the binding or loss of copper to MNKr2 does not cause substantial changes to the secondary structure of the protein.  (+info)

Biophysical characterization of a designed TMV coat protein mutant, R46G, that elicits a moderate hypersensitivity response in Nicotiana sylvestris. (8/11120)

The hypersensitivity resistance response directed by the N' gene in Nicotiana sylvestris is elicited by the tobacco mosaic virus (TMV) coat protein R46G, but not by the U1 wild-type TMV coat protein. In this study, the structural and hydrodynamic properties of R46G and wild-type coat proteins were compared for variations that may explain N' gene elicitation. Circular dichroism spectroscopy reveals no significant secondary or tertiary structural differences between the elicitor and nonelicitor coat proteins. Analytical ultracentrifugation studies, however, do show different concentration dependencies of the weight average sedimentation coefficients at 4 degrees C. Viral reconstitution kinetics at 20 degrees C were used to determine viral assembly rates and as an initial assay of the rate of 20S formation, the obligate species for viral reconstitution. These kinetic results reveal a decreased lag time for reconstitution performed with R46G that initially lack the 20S aggregate. However, experiments performed with 20S initially present reveal no detectable differences indicating that the mechanism of viral assembly is similar for the two coat protein species. Therefore, an increased rate of 20S formation from R46G subunits may explain the differences in the viral reconstitution lag times. The inferred increase in the rate of 20S formation is verified by direct measurement of the 20S boundary as a function of time at 20 degrees C using velocity sedimentation analysis. These results are consistent with the interpretation that there may be an altered size distribution and/or lifetime of the small coat protein aggregates in elicitors that allows N. sylvestris to recognize the invading virus.  (+info)

Request Report Methodology @ https://www.persistencemarketresearch.co/methodology/26926 The content of the study subjects, includes a total of 15 chapters:. Chapter 1, to describe Vibrational Circular Dichroism Spectrometer product scope, market overview, market opportunities, market driving force and market risks.. Chapter 2, to profile the top manufacturers of Vibrational Circular Dichroism Spectrometer , with price, sales, revenue and global market share of Vibrational Circular Dichroism Spectrometer in 2017 and 2020.. Chapter 3, the Vibrational Circular Dichroism Spectrometer competitive situation, sales, revenue and global market share of top manufacturers are analyzed emphatically by landscape contrast.. Chapter 4, the Vibrational Circular Dichroism Spectrometer breakdown data are shown at the regional level, to show the sales, revenue and growth by regions, from 2014 to 2020.. Chapter 5, 6, 7, 8 and 9, to break the sales data at the country level, with sales, revenue and market share for ...
Research Corridor has published a new research study titled Circular Dichroism Spectroscopy Market - Growth, Share, Opportunities, Competitive Analysis and Forecast, 2017 - 2025. The Circular Dichroism Spectroscopy Market report studies current as well as future aspects of the Circular Dichroism Spectroscopy Market based upon factors such as market dynamics, key ongoing trends and segmentation analysis. Apart from the above elements, the Circular Dichroism Spectroscopy Market research report provides a 360-degree view of the Lipstick Packing industry with geographic segmentation, statistical forecast and the competitive landscape.. Browse the complete report at http://www.researchcorridor.com/circular-dichroism-spectroscopy-market/. Geographically, the Circular Dichroism Spectroscopy Market report comprises dedicated sections centering on the regional market revenue and trends. The Circular Dichroism Spectroscopy Market has been segmented on the basis of geographic regions into North America, ...
O:13:\PanistOpenUrl\:36:{s:10:\\u0000*\u0000openUrl\;N;s:6:\\u0000*\u0000idc\;N;s:6:\\u0000*\u0000fmt\;s:7:\journal\;s:6:\\u0000*\u0000doi\;s:0:\\;s:6:\\u0000*\u0000pii\;s:0:\\;s:7:\\u0000*\u0000pmid\;s:0:\\;s:9:\\u0000*\u0000atitle\;s:99:\CIRCULAR DICHROISM STUDIES ON ALPHA 2-ANTIPLASMIN AND ITS INTERACTIONS WITH PLASMIN AND PLASMINOGEN\;s:9:\\u0000*\u0000jtitle\;s:0:\\;s:9:\\u0000*\u0000stitle\;s:0:\\;s:7:\\u0000*\u0000date\;s:4:\1982\;s:9:\\u0000*\u0000volume\;s:0:\\;s:8:\\u0000*\u0000issue\;s:0:\\;s:8:\\u0000*\u0000spage\;s:0:\\;s:8:\\u0000*\u0000epage\;s:0:\\;s:8:\\u0000*\u0000pages\;s:0:\\;s:7:\\u0000*\u0000issn\;s:0:\\;s:8:\\u0000*\u0000eissn\;s:0:\\;s:9:\\u0000*\u0000aulast\;s:7:\NILSSON\;s:10:\\u0000*\u0000aufirst\;s:1:\T\;s:9:\\u0000*\u0000auinit\;N;s:10:\\u0000*\u0000auinitm\;N;s:5:\\u0000*\u0000au\;a:3:{i:0;s:9:\NILSSON T\;i:1;s:10:\SJOEHOLM I\;i:2;s:7:\WIMAN ...
TY - JOUR. T1 - Structures, vibrational absorption and vibrational circular dichroism spectra of L-alanine in aqueous solution: a density functional theory and RHF study. AU - Frimand, K.. AU - Bohr, H.. AU - Jalkanen, K.J.. AU - Suhai, S.. PY - 2000. Y1 - 2000. M3 - Article. VL - 255. SP - 165. EP - 194. JO - Chemical Physics. JF - Chemical Physics. SN - 0301-0104. ER - ...
A 4 : 1 (v/v) mixture of CD3CN and C6F6 was gelated by a chiral low-molecular weight gelator, N,N′-diperfluoroheptanoyl-1,2(R,R)- or -1,2(S,S)-diaminocyclohexanel. The process from a viscous isotropic solution to a stable gel was monitored by vibrational circular dichroism (VCD) spectroscopy. The transient VCD spec
The relative configuration of a key subunit of hemicalide, a recently isolated, highly bioactive marine natural product having potent antiproliferative activity against a panel of human cancer cell lines, was assigned by combining stereocontrolled synthesis of model substrates with NMR, IR, and vibrational circular dichroism (VCD) spectroscopy. The assignment of the absolute configuration of asymmetric carbon center C42 in two structurally complex epimeric substructures containing six stereocenters by VCD analysis illustrates the power and reliability of combining methods. ...
Circular Dichroism and Magnetic Circular Dichroism Spectroscopy for Organic Chem... Circular Dichroism and Magnetic Circular Dichroism Spectroscopy for Organic Chem... ...
Nicu, V. and Neugebauer, J. and Wolff, S. and Baerends, E. 2008. A vibrational circular dichroism implementation within a Slater-type-orbital based density functional framework and its application to hexa- and hepta-helicenes. Theoretical Chemistry Accounts: Theory, Computation, and Modeling (Theoretica Chimica Acta). 119: pp. 245-263 ...
2017-2022 Southeast Asia and Regional Vibrational Circular Dichroism Spectrometer Industry Production, Sales and Consumption Status and Prospects Professional Market Research Report
Circular dichroism measurements of ArsTM (purple) and MamAΔ41 proteins from RS-1 (Blue) Mbav (orange), AMB-1 (green) and MSR-1 (red).(A) Circular dichroism spe
Bertucci C., Cimitan S., Riva A., Morazzoni P.. The binding to human serum albumin (HSA) of the antitumoural drug paclitaxel and of several structural analogues has been characterized by bioaffinity chromatography and circular dichroism. A ranking of the taxanes was obtained for their affinity to the protein by measuring their retention times on an albumin chromatographic column. This also allowed the calculation of the drug bound percentage. Affinity resulted significantly affected by the nature of the isoserinic side chain, the presence of the 1,14-carbonate moiety and the substituent at C-7, showing that the hydrophobicity of the drug is fundamental in the binding process. The analysis demonstrated that the organic solvent highly alters the interaction mechanism of taxanes to the protein and so the affinity results. Circular dichroism experiments supported this hypothesis. Furthermore, taxanes binding to the serum carrier was characterized by displacement chromatography, by adding into the ...
TY - JOUR. T1 - Measurement of circular dichroism of ferroelectric fresnoite Ba 2Si2TiO8. AU - Asahi, Toni. AU - Osaka, Tetsuya. AU - Abrahamsb, Sidney C.. AU - Matsuki, Ryo. AU - Asai, Hiroshi. AU - Nanamatsu, Satoshi. AU - Kobayashi, Jinzo. PY - 2003/12/1. Y1 - 2003/12/1. N2 - The urgent goal of the optical polarimetry of solids is simultaneous and accurate measurements of circular dichroism together with circular birefringence. Needless to say, measurements of circular phenomena are extremely difficult compared with those of linear ones. As for circular birefringence, 170 years elapsed since its discovery by Arago until the development of the HAUP method, by which the accurate measurements of the gyration tensor components of a solid became possible for the first time. Subsequent to appearance of the HAUP method, attempts to extend the HAUP theory to being applicable for measurements of circular dichroism of crystals were followed by several authors. However any applications to real crystal ...
TY - JOUR. T1 - Tunable circular dichroism and valley polarization in the modified Haldane model. AU - Vila, Marc. AU - Hung, Nguyen Tuan. AU - Roche, Stephan. AU - Saito, Riichiro. PY - 2019/4/16. Y1 - 2019/4/16. N2 - © 2019 American Physical Society. We study the polarization dependence of optical absorption for a modified Haldane model, which exhibits antichiral edge modes in the presence of sample boundaries and has been argued to be realizable in transition metal dichalcogenides or Weyl semimetals. A rich optical phase diagram is unveiled, in which the correlations between perfect circular dichroism, pseudospin andvalley polarization can be tuned independently upon varying the Fermi energy. In particular, perfect circular dichroism and valley polarization are achieved simultaneously. This combination of optical properties suggests some interesting photonic device functionality (e.g., light polarizer) which could be combined with valleytronics applications (e.g., generation of valley ...
The preparations of pentaamminecobalt(III) complexes of a series of asymmetric unidentate amines are reported. The circular dichroism spectra for the (S)-amines show negative Cotton effects under the A → T absorption band, which are somewhat solvent dependent but independent of temperature down to -190°C. The vicinal effect from 1-cyclohexylethylamine was comparable to that from analogous aromatic compounds, but the simpler alkyl derivatives were found to impose smaller rotational strengths on the d-d transitions. The lack of measurable c.d. in the visible region for tetraammine((S)-butane-1, 3-diamine)cobalt(III) is reported and discussed in the light of the other studies ...
O:13:\PanistOpenUrl\:36:{s:10:\\u0000*\u0000openUrl\;N;s:6:\\u0000*\u0000idc\;N;s:6:\\u0000*\u0000fmt\;s:7:\journal\;s:6:\\u0000*\u0000doi\;s:0:\\;s:6:\\u0000*\u0000pii\;s:0:\\;s:7:\\u0000*\u0000pmid\;s:0:\\;s:9:\\u0000*\u0000atitle\;s:92:\CIRCULAR DICHROISM STUDIES OF CYANATE-INDUCED CONFORMATIONAL CHANGES IN HEMOGLOBINS A AND S.\;s:9:\\u0000*\u0000jtitle\;s:0:\\;s:9:\\u0000*\u0000stitle\;s:0:\\;s:7:\\u0000*\u0000date\;s:4:\1976\;s:9:\\u0000*\u0000volume\;s:0:\\;s:8:\\u0000*\u0000issue\;s:0:\\;s:8:\\u0000*\u0000spage\;s:0:\\;s:8:\\u0000*\u0000epage\;s:0:\\;s:8:\\u0000*\u0000pages\;s:0:\\;s:7:\\u0000*\u0000issn\;s:0:\\;s:8:\\u0000*\u0000eissn\;s:0:\\;s:9:\\u0000*\u0000aulast\;s:6:\SIMONS\;s:10:\\u0000*\u0000aufirst\;s:2:\ER\;s:9:\\u0000*\u0000auinit\;N;s:10:\\u0000*\u0000auinitm\;N;s:5:\\u0000*\u0000au\;a:4:{i:0;s:9:\SIMONS ER\;i:1;s:11:\HARTZBAND P\;i:2;s:8:\WHITIN J\;i:3;s:9:\CHAPMAN ...
Read Secondary Structure of Proteins Through Circular Dichroism Spectroscopy, Annual Review of Biophysics on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Philip J. Stephens was a theoretical chemist who brought to fruition two new forms of optical spectroscopy, using circularly polarized light, for the determination of electronic structure and molecular stereochemistry. The first was magnetic circular dichroism (MCD), the wavelength dependence of the differential absorption of left and right circularly polarized light induced by a magnetic field applied parallel to the light beam. Stephens established a methodology for extracting from MCD spectra the angular momentum characteristics of ground and excited electronic states and demonstrated applications to the assignment of the optical spectra of coordination complexes of transition metals and to metalloproteins. In the second half of his career Stephens led the field of vibrational circular dichroism (VCD), the measurement of the natural circular dichroism (CD) arising from the vibrational transitions of chiral molecules. He developed instrumental techniques to measure this weak dichroism over a ...
Circular Dichroism (CD) analysis from SGS - meet regulatory requirements with class-leading CD spectroscopic analysis, alpha helix beta sheet analysis, and secondary and tertiary structure analysis. Learn more.
The circular-dichroism and proton-magnetic-resonance spectra of complexes of ribonuclease A with dihydrouridine 3′-phosphate, 2′- and 3′-CMP, arabinosyl-3′-CMP, 1-(2-hydroxyethyl)cytosine 2′-phosphate and 1-(3-hydroxypropyl)cytosine 3′-phosphate were studied. Comparison of the results shows that non-additivity of the circular-dichroic spectrum of an enzyme-nucleotide complex may be due to: (a), alteration of the circular dichroic spectrum of the nucleotide under the influence of the asymmetric protein matrix (induced dichroism), and (b) a change in the nucleotide conformation. The contribution of each of the two factors was estimated to calculate the circular-dichoroic spectra of 2′-CMP and 3′-CMP in complex with ribonuclease A. 3′-CMP in this complex was characterized by negative circular dichroism in the long-wavelength absorption band of the nucleotide, whereas 2′-CMP was characterized by positive circular dichroism. Since both nucleotides in the complex are known to be in ...
The circular-dichroism and proton-magnetic-resonance spectra of complexes of ribonuclease A with dihydrouridine 3′-phosphate, 2′- and 3′-CMP, arabinosyl-3′-CMP, 1-(2-hydroxyethyl)cytosine 2′-phosphate and 1-(3-hydroxypropyl)cytosine 3′-phosphate were studied. Comparison of the results shows that non-additivity of the circular-dichroic spectrum of an enzyme-nucleotide complex may be due to: (a), alteration of the circular dichroic spectrum of the nucleotide under the influence of the asymmetric protein matrix (induced dichroism), and (b) a change in the nucleotide conformation. The contribution of each of the two factors was estimated to calculate the circular-dichoroic spectra of 2′-CMP and 3′-CMP in complex with ribonuclease A. 3′-CMP in this complex was characterized by negative circular dichroism in the long-wavelength absorption band of the nucleotide, whereas 2′-CMP was characterized by positive circular dichroism. Since both nucleotides in the complex are known to be in ...
Circular dichroism (CD) spectroscopy is an optical technique that measures the difference in the absorption of left and right circularly polarized light. This technique has been widely employed in the studies of nucleic acids structures and the use of it to monitor conformational polymorphism of DNA has grown tremendously in the past few decades. DNA may undergo conformational changes to B-form, A-form, Z-form, quadruplexes, triplexes and other structures as a result of the binding process to different compounds. Here we review the recent CD spectroscopic studies of the induction of DNA conformational changes by different ligands, which includes metal derivative complex of aureolic family drugs, actinomycin D, neomycin, cisplatin, and polyamine. It is clear that CD spectroscopy is extremely sensitive and relatively inexpensive, as compared with other techniques. These studies show that CD spectroscopy is a powerful technique to monitor DNA conformational changes resulting from drug binding and also
A new method to detect the vibrational CD (VCD) of a localized part of a chiral mol. system is reported. A local VCD amplifier was implemented, and the distance dependence of the amplification was investigated in a series of peptides. The results indicate a characteristic distance of 2.0±0.3 bonds, which suggests that the amplification is a localized phenomenon. The amplifier can be covalently coupled to a specific part of a mol., and can be switched ON and OFF electrochem. By subtracting the VCD spectra obtained when the amplifier is in the ON and OFF states, the VCD of the local environment of the amplifier can be sepd. from the total VCD spectrum. Switchable local VCD amplification thus makes it possible to zoom in on a specific part of a chiral mol ...
A series of polypeptides with well-defined sequences, (Asp3Phe1)n, (Asp2Phe1)n, (Asp1Phe1)n, (Asp1Phe2)n, and (Asp1Phe3)n, containing the hydrophilic amino acid, aspartic acid (Asp) and the hydrophobic amino acid, phenylalanine (Phe), were synthesized. Their behaviour in aqueous solution was investigated by performing fluorescence quenching and non-radiative energy transfer (NRET) experiments which were complemented by dynamic (DLS) and static (SLS) light scattering. The photophysical properties of the polypeptides were dependent on their Phe content. An increase in the Phe content led to an increase in the extinction coefficient, fluorescence quantum yield, and fluorescence average lifetime of the polypeptides. Circular dichroism experiments revealed that except for the (Asp1Phe3)n polypeptide, which adopts an alpha-helical conformation in aqueous solution, the other polypeptides did not adopt any known conformation in solution. The fluorescence quenching studies performed using molecular ...
Circular dichroism (CD) is a powerful technique for studying the structures of proteins in solution, as well as structural changes that may occur when proteins bind to ligands
The Aviv Circular Dichroism Spectrometer, model 215, records CD as a function of wavelength, time, temperature, pH and concentration using a double monochromator containing two UV grade prisms as dispersing elements. Right and left circularly polarized light is produced by a 50 KHz photoelastic modulator.. The instrument includes software by AVIV for instrument control, data acquisition and processing in a Windows environment.. ...
Introducing one or two alkynyl-iron moieties onto a carbo[6]helicene results in organometallic helicenes (2 a,b) that display strong chiroptical activity combined with efficient redox-triggered switching. The neutral and oxidized forms have been studied in detail by electronic and vibrational circular dichroism, as well as by Raman optical activity (ROA) spectroscopy. The experimental results were analyzed and spectra were assigned with the help of first-principles calculations. In particular, a recently developed method for ROA calculations under resonance conditions has been used to study the intricate resonance effects on the ROA spectrum of mono-iron ethynylhelicene 2 a.
Ferromagnetic amorphous oxides in the EuO-TiO[2] system studied by the Faraday effect in the visible region and the x-ray magnetic circular dichroism at the Eu M[4,5] and L[2,3] edges (2013 ...
J. P. Rueff, Galera, R. M., Pizzini, S., Fontaine, A., Garcia, L. M., Giorgetti, C., Dartyge, E., and Baudelet, F., X-ray magnetic circular dichroism at the Gd L edges in Gd-Ni-Co amorphous systems, PHYSICAL REVIEW B, vol. 55, pp. 3063-3070, 1997. ...
The p53, p63 and p73 proteins belong to the p53 family of transcription factors, playing key roles in tumour suppression. The α-splice variant of p73 (p73α) has at its C terminus a sterile alpha motif (SAM); this domain, SAMp73, formed by five helices (α1 to α5), is thought to mediate in protein-protein interactions. The E3-ligase MDM2 binds to p73 at its N terminus transactivation domain (TA), bu ...
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Department of Chemistry, UBC Faculty of Science. Vancouver Campus. 2036 Main Mall. Vancouver, BC Canada V6T 1Z1. Tel: 604.822.3266. Fax: 604.822.2847. ...
Herein we describe a straightforward method for the determination of the absolute con?guration of 3-indolyl(bromo)acetate 7, 3-indolyl(alkoxy)acetates 8a?f and 3-indolyl(amino)acetate 8g, based on 1HNMR spectral analysis. The conformational preferences for two diastereomeric esters were calculatedby DFT, which matched very well with the experimental results. X-ray diffraction analysis allowed usto validate the methodology, and independent absolute con?guration evidence was obtained by vibrational circular dichroism. ...
The focus of my PhD has been the development of new instrumentation for UV polarised spectroscopic methods, especially that of circular dichroism (CD). Whilst instrument design is an unusual field of research (especially for a chemist!) I feel it is one of great importance to the scientific process. The instruments that scientists employ to study the world around us are our eyes and ears, enabling us to view objects from the angstrom level to distant galaxies. In many ways, it could be argued that the limiting factor for the majority of research is the methods that we can employ. By improving these methods, we naturally enhance the quality of our research and further understanding. Capillary circular dichroism (CaCD). One of the key problems with circular dichroism as a technique is its relatively high sample requirments for each reading. In order to address this problem, we have been working on using extruded quartz capillaries as an inexpensive alternative sample holder to the rectangular ...
The extension of circular dichroism measurements into the VUV region was motivated by the need to provide supplementary techniques to the absorption studies lacking rotational and vibrational structure. CD as one of these techniques is determined by selection rules which differ from those in absorption and thus enables the assignment of excited electronic states. Another application of VUVCD measurements is the use of correlation between the sign of the CD signal and the absolute configuration to formulate sector rules of chromophores absorbing only in the VUV. In this review we will present results obtained on our home-made instrument capable of measuring CD signal down to 150 nm. These applications of VUVCD will be demonstrated in systems such as the oxirane chromophore, the methylenecyclohexene chromophore and the amine chromophore. The role of singlet-triplet transitions and their contribution to CD and ORD will also be discussed. ...
A relatively new technique with a growing number of applications, dichroism spectroscopy is based upon the fact that a sample interacts with the same wavelength of light having different circular polarization in different ways.
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This Jasco J-815 CD System comes loaded with accessories including a 6-sample Peltier Turret Cell Changer (Model MPTC-490S/15), a Julabo Circulating Water Bath
We are a biophysical chemistry group that is focused on understanding the mechanism of entry by viruses enveloped by a membrane. Many important human pathogens are enveloped viruses, including Human Immunodeficiency (HIV), Influenza, Measles, Rabies, West Nile, Zika, Ebola, SARS, and MERS. Each virus has evolved a protein in its membrane that catalyzes the joining (fusion) of the virus membrane with the membrane of the target cell. We are studying the glycoprotein 41 kDa (gp41) fusion protein of HIV and hemagglutinin subunit 2 (HA2) fusion protein of influenza. Our work and contributions include the protein structures and locations in membrane. We also study the changes in membrane structure associated with the protein. A significant fraction of our effort is in development and application of solid-state, i.e. anisotropic nuclear magnetic resonance (NMR) to these proteins. We also apply a variety of other biophysical methods including circular dichroism spectroscopy, fluorescence ...
Phase-modulated ellipsometry of the J-aggregates of the title porphyrin shows that the material gives a true CD signal. This confirms that there is a real chiral transfer by mechanical forces, mediated by shear gradient flows, from the macroscopic to the electronic transition level. Dislocations in the structure of the aggregate could justify the formation of chirality at the level of the electronic transitions once the mesophases can be sculptured by hydrodynamic gradient flows ...
In this report, we provide an x-ray magnetic circular dichroism (XMCD) study for 100?nm thick epitaxial magnetite (Fe3O4) films on MgO (001) and Al2O3 (0001) substrates. For XMCD, we recorded the surface sensitive total electron yield and the bulk sensitive transmission spectra. From the analysis of the XMCD data, we find an increased Fe spin moment (10% larger) at the surface of the film on MgO (100) with respect to the corresponding bulk value of the film. Surface and bulk spin moments of the film on Al2O3(0001) are almost equal. For both films, the bulk orbital to spin moments ratio increases from zero at 70?K to 0.03?0.04 at 300?K. For Fe3O4/MgO (001), the surface orbital to spin moment ratio behaves similarly to the bulk value, while the orbital to spin moments ratio is increased at the Fe3O4/Al2O3 (0001) surface to 0.06. The observed differences between films grown on MgO and Al2O3 are explained within the framework of differences in mismatch strain experienced by the films. ...
E. Dartyge, Baudelet, F., Giorgetti, C., and Odin, S., X-ray magnetic circular dichroism as a tool for magnetic studies of 4f compounds, JOURNAL OF ALLOYS AND COMPOUNDS, vol. 275, pp. 526-532, 1998. ...
But a city government cant achieve this alone. It needs the participation of citizens to make circularity a reality!. The circular economy discourse is mostly techno-centric and currently lacks a societal dimension. We are broadening the circular economy framework, and inviting you, your neighbour and your curious nephew to participate in the transition. So, what can you do? What actions can YOU take to advance the urban transition to a circular model? This is what the Circular Citizen Challenge is all about. ​. During February - the month of circular consumption - we invite you to explore together how Utrechters can shape the circular future of our city and our lives in it. ...
On the basis of temperature-dependent UV-vis and circular dichroism (CD) spectroscopy measurements, we observed that C3-symmetrical discotic molecules, chiral (R)-1 and achiral 2, both self-assemble in a highly cooperative fashion. Chiral (R)-1 shows a higher degree of cooperativity, meaning it requ …
Biophotonics. Bioelectromagnetics. Calcium imaging. Calorimetry. Chromatography. Circular dichroism. Computational chemistry. Cryobiology. Dual-polarization. .
The CD spectra of d(GGGGTTTTGGGG) and d(TGGGGTTGGGGT) in the presence of 5 and 140 mM sodium are shown in the upper panels. The CD spectra of these DNAs in the
Circular Dichroism, DNA, Absorption, Nickel, Z-dna, B-dna, Report, Spermine, Cadmium, Concentrations, Cytosine, Guanine, Fluorescence, Ionic Strength, Oligonucleotides, Adenine, Electronic, Light, Porphyrins, Sensitivity
Kinase, Lipids, Membrane, Liposomes, Membranes, Micelles, Rapamycin, Association, Circular Dichroism, Concentrations, Environment, Fkbp12, Growth, Magnetic, Magnetic Resonance, Membrane Protein, Metabolism, Neurological Disorders, Nuclear Magnetic Resonance, Phosphatidic Acid
article{9c427bbf-e48d-404f-aa8d-386a5072b5fd, abstract = {,p,Apolipoprotein A-I (apoA-I) in high-density lipoprotein (HDL) provides cardiovascular protection. Synchrotron radiation circular dichroism (SRCD) spectroscopy was used to analyze the dynamic solution structure of the apoA-I protein in the apo- and HDL-states and the protein structure conversion in HDL formation. Wild-type apoA-I protein was compared to human variants that either are protective (R173C, Milano) or lead to increased risk for ischaemic heart disease (A164S). Comparable secondary structure distributions in the HDL particles, including significant levels of beta strand/turn, were observed. ApoA-I Milano in HDL displayed larger size heterogeneity, increased protein flexibility, and an altered lipid-binding profile, whereas the apoA-I A164S in HDL showed decrease thermal stability, potentially linking the intrinsic HDL propensities of the variants to disease risk.,/p,}, articleno = {13540}, author = {Giudice, Rita Del and ...
New advances in instrumentation, demonstration of proof-of-principle studies, and development of new tools and methods for data analysis and interpretation have enabled the technique of Synchrotron Radiation Circular Dichroism (SRCD) spectroscopy to become a useful tool for structural and functional biology. This paper discusses the characterisation of two new SRCD beamlines, CD1 at the Institute for Storage Rings (ISA), Denmark and 4B8 at the Beijing Synchrotron Radiation Facility (BSRF), China, and new applications of the method for examining biological systems ...
We present here a study of the magnetic properties of the Nd2−xHoxFe14B series. The macroscopic properties of these compounds evolve continuously from those of Nd2Fe14B to those of Ho2Fe14B as Ho gradually replaces Nd. The system shows a compensation of the rare-earth sublattice magnetization for a critical concentration, xc=0.55, that is reflected into the anomalous behavior of both macroscopic and microscopic magnetic probes. The combined analysis of magnetization, 57Fe Mössbauer spectroscopy and Fe K-edge x-ray magnetic circular dichroism (XMCD) measurements suggests that the origin of the anomalous magnetic behavior found at xc=0.55 is mainly due to the Ho sublattice. Moreover, the analysis of the Fe K-edge XMCD signals reveal the presence of a rare-earth contribution, reflecting the coupling of the rare-earth and Fe magnetic moments, which can lead to the possibility of disentangling the magnetic behavior of both Fe and R atoms using a single absorption edge ...
The Protein Circular Dichroism Data Bank (PCDDB) is a web-based resource containing circular dichroism (CD) and synchrotron radiation circular dichroism spectral and associated metadata located at http://pcddb.cryst.bbk.ac.uk. This resource provides a freely available, user-friendly means of accessing validated CD spectra and their associated experimental details and metadata, thereby enabling broad usage of this material and new developments across the structural biology, chemistry, and bioinformatics communities. The resource also enables researchers utilizing CD as an experimental technique to have a means of storing their data at a secure site from which it is easily retrievable, thereby making their results publicly accessible, a current requirement of many grant-funding agencies world-wide, as well as meeting the data-sharing requirements for journal publications. This tutorial provides extensive information on searching, accessing, and downloading procedures for those who wish to utilize ...
TY - CHAP. T1 - Structure and electronic circular dichroism of chiral porphyrins and chiral porphyrin dimers. AU - Balaz, Milan. AU - Joh, Yoonbin A.. AU - Varga, Krisztina. AU - Berova, Nina. AU - Purrello, Roberto. AU - DUrso, Alessandro. PY - 2019/1/1. Y1 - 2019/1/1. UR - http://www.scopus.com/inward/record.url?scp=85068063365&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=85068063365&partnerID=8YFLogxK. U2 - 10.1142/9789811201813_0003. DO - 10.1142/9789811201813_0003. M3 - Chapter. AN - SCOPUS:85068063365. SN - 9789811201806. SP - 205. EP - 284. BT - Handbook of Porphyrin Science. PB - World Scientific Publishing Co.. ER - ...
MILLS, M.; LIN, C.; CHAUHAN, M. and KLUMP, H.H.. Circular dichroism as a means to follow DNA gymnastics: on the shoulders of giants. S. Afr. j. sci. [online]. 2009, vol.105, n.7-8, pp.309-311. ISSN 1996-7489.. This is the first report of DNA stem-loops self-assembled by foot-loop interactions into either two-dimensional strings or three-dimensional spirals, distinguished by circular dichroism spectroscopy. All subunits are linked by cooperative Watson-Crick hydrogen bonds.. Keywords : DNA; circular dichroism; nanostructures; stem-loop structures; self-assembly. ...
TY - GEN. T1 - Circular dichroism effects in nonlinear-optical response of planar chiral metamaterials. AU - Mamonov, E. A.. AU - Kolmychek, I. A.. AU - Vandendriessche, S.. AU - Hojeij, M.. AU - Ekinci, Y.. AU - Valev, V. K.. AU - Verbiest, T.. AU - Murzina, T. V.. PY - 2013/1/1. Y1 - 2013/1/1. N2 - Circularly polarized optical second harmonic generation (SHG) is studied experimentally in regular arrays of planar G-shaped nanostructures. Circular dichroism in SHG is observed and the sign of the effect reverses with the handedness of the nanostructures, which is a signature of chirality in the nonlinear optical response.. AB - Circularly polarized optical second harmonic generation (SHG) is studied experimentally in regular arrays of planar G-shaped nanostructures. Circular dichroism in SHG is observed and the sign of the effect reverses with the handedness of the nanostructures, which is a signature of chirality in the nonlinear optical response.. UR - ...
Angewandte Chemie DOI: 10.1002/ange.201006255 H2 Activation Iron-Chromophore Circular Dichroism of [Fe]-Hydrogenase: The Conformational Change Required for H2 Activation** Seigo Shima,* Sonja Vogt, Andreas Gbels, and Eckhard Bill Circular dichroism (CD) spectroscopy is a very sensitive method used to detect changes in inherently chiral chromophores and in achiral chromophores embedded in chiral surroundings.[1] Information on the secondary structures of proteins can be obtained by CD spectroscopy in the far-UV spectral region (190-250 nm), and CD in the near-UV region (250-350 nm) can be sensitive to certain aspects of tertiary structure.[1b] Near-UV and visible CD spectroscopy is also used to analyze metal complexes[1a,c] and the active site of metal-containing enzymes, such as P450 (300-500 nm), galactose oxidase (300-700 nm), and biotin sulfoxide reductase (300-650 nm).[2] The [Fe]-hydrogenase found in many methanogenic archaea catalyzes the reversible reduction of ...
The temperature sensitivity of vaccines and therapeutic proteins forces the distribution of life-saving treatments to rely heavily on the temperature-controlled (usually 2-8 degrees C) supply and distribution network known as the cold chain. Here, using avidin as a model, we demonstrate how surface engineering could significantly increase the thermal stability of therapeutic proteins. A combination of spectroscopic (Fourier transform infrared, circular dichroism, and ultraviolet-visible) and scattering techniques (dynamic light scattering, small-angle, and wide-angle X-ray scattering) were deployed to probe the activity, structure, and stability of the model protein. Temperature-dependent synchrotron radiation circular dichroism spectroscopy was used to demonstrate a significant increase in thermal stability, with a half denaturation temperature of 139.0 degrees C and reversible unfolding with modified avidin returning to a 90% folded state when heated to temperatures below 100 degrees C. ...
We compared various spectroscopic properties of a norfloxacin-single stranded DNA complex with those of norfloxacin-double stranded DNA complex. Norfloxacin binds to both double- and single stranded DNA, and we observed the following spectroscopic changes for both complexes: hypochromism in the norfloxacin absorption region in the absorption spectrum, the characteristic induced CD spectrum consisting of a weak positive band at 323 nm and a strong positive band at 280-300 nm followed by a negative band in the 260 nm region, a strong decrease in the fluorescence intensity and a red-shift in the fluorescence emission spectrum, and shorter fluorescence decay times. These results indicate that the environments of the bound norfloxacin in both DNAs are similar, although the equilibrium constant of the norfloxacin-single stranded DNA was twice as high as the norfloxacin-double stranded DNA complex. Both complexes were thermodynamically favored with similar negative ΔGo. Negative ΔHo terms contribute ...
Sutherland, J. C., Dec 1 2009, Modern Techniques for Circular Dichroism and Synchrotron Radiation Circular Dichroism Spectroscopy. Wallace, B. A. & Janes, R. W. (eds.). p. 19-72 54 p. (Advances in Biomedical Spectroscopy; vol. 1).. Research output: Chapter in Book/Report/Conference proceeding › Chapter ...
TY - JOUR. T1 - Erratum to X-ray absorption spectroscopy and magnetic circular dichroism in codeposited C60-Co films with giant tunnel magnetoresistance [Chem. Phys. Lett. 470 (2009) 244] (DOI:10.1016/j.cplett.2009.01.032). AU - Matsumoto, Yoshihiro. AU - Sakai, Seiji. AU - Takagi, Yasumasa. AU - Nakagawa, Takeshi. AU - Yokoyama, Toshihiko. AU - Shimada, Toshihiro. AU - Mitani, Seiji. AU - Takanashi, Koki. AU - Naramoto, Hiroshi. AU - Maeda, Yoshihito. N1 - Funding Information: 60 -Co films with giant tunnel magnetoresistance [Chem. Phys. Lett. 470 (2009) 244] Yoshihiro Matsumoto a ⁎ [email protected] Seiji Sakai a Yasumasa Takagi b Takeshi Nakagawa b Toshihiko Yokoyama b Toshihiro Shimada c Seiji Mitani d Koki Takanashi a d Hiroshi Naramoto a Yoshihito Maeda a e a Advanced Science Research Center, Japan Atomic Energy Agency, 2-4 Shirakata-Shirane, Tokai, Naka, Ibaraki 319-1195, Japan b Department of Materials Molecular Science, Institute for Molecular Science, Okazaki, Aichi ...
The binding of 2,5-bis(4-amidinophenyl)furan (APF) to calf thymus DNA, [poly(dA-dT)]2, and [poly(dG-dC)]2 has been studied with flow linear dichroism and circular dichroism spectroscopy. The electronic excited states of the APF chromophore were first characterized using experimental and quantum mechanical methods: it is shown that the low-energy absorption band (320-400 nm) originates from only a single electronic transition which is polarized along the long axis of the molecule, information that is crucial for the structural interpretation of the linear and circular dichroism spectra of the APF-DNA complexes. By contrast, in the unsymmetric analogue 4,6-diamidino-2-phenylindole (DAPI) two overlapping transitions, with somewhat divergent polarizations, both contribute to the first absorption band. Upon binding to DNA the spectroscopic behavior of APF strongly resembles that of DAPI. The linear dichroism data show that the drug binds to calf thymus DNA and [poly(dA-dT)]2 with an angle of 46-degrees +/-
The gadolinium-based nanoagent named AGuIX ® is a unique radiosensitizer and contrast agent which improves the performance of radiotherapy and medical imaging. Currently tested in clinical trials, AGuIX ® is administrated to patients via intravenous injection. The presence of nanoparticles in the blood stream may induce harmful effects due to undesired interactions with blood components. Thus, there is an emerging need to understand the impact of these nanoagents when meeting blood proteins. In this work, the influence of nanoagents on the structure and stability of the most abundant blood protein, human serum albumin, is presented. Synchrotron radiation circular dichroism showed that AGuIX ® does not bind to the protein, even at the high ratio of 45 nanoparticles per protein at 3 mg/L. However, it increases the stability of the albumin. Isothermal thermodynamic calorimetry and fluorescence emission spectroscopy demonstrated that the effect is due to preferential hydration processes. Thus, this study
Ligand binding properties of five single amino acid substituted variants (V11A, D12A, Q15A, Q15E, and F16A) of human insulin-like growth factor I (IGF-I) were analyzed with respect to their binding affinities and binding kinetics to recombinant IGF binding protein 1 (IGFBP-1) and a soluble form of the IGF type I receptor (sIGF-I(R)), respectively. Side chains of the substituted residues are all predicted to be the most surface exposed in the alpha-helical portion of the B-region of the IGF-I molecule. The IGF-I variants were produced as fusion proteins to a IgG(Fc) binding protein domain, Z. Ligand binding kinetic rates were determined using BIAcore biosensor interaction analysis technology. All IGF-I variants showed altered binding affinities to both IGFBP- I and sIGF-I(R). Secondary structure content of the IGF-I variants was estimated using far-UV circular dichroism spectroscopy, followed by variable selection secondary structure calculations. The amount of calculated alpha-helicity is ...
Day, L., Zhai, J., Xu, M., Jones, N. C., Hoffmann, S. V., & Wooster, T. J. (2014). Conformational changes of globular proteins adsorbed at oil-in-water emulsion interfaces examined by synchrotron radiation circular dichroism. Food Hydrocolloids, 34, 78-87. doi: 10.1016/j.foodhyd.2012.12.015.. Zhai, J. L., Day, L., Aguilar, M. I., & Wooster, T. J. (2013). Protein folding at emulsion oil/water interfaces. Current Opinion in Colloid and Interface Science, 18(4), 257-271. doi: 10.1016/j.cocis.2013.03.002.. ...
A powerful tool used to monitor secondary structure is circular dichroism (CD), which is the differential absorbance of left and right circularly polarized light. Since proteins consist of chiral amino acids, they are CD active and exhibit distinct CD spectra that are sensitive to the proteins secondary structure. This spectra can provide a qualitative snapshot of the protein structure or the secondary structure composition of a protein can be estimated from the far UV CD spectrum using a number of algorithms, several of which are made available in Olis GlobalWorks software.. Protein secondary structure can also be used to monitor protein folding and unfolding. An equilibrium study consists of collection of CD spectra as a function of increasing temperature or chemical denaturant. As the protein unfolds, the CD spectrum reflects this. Fitting data at a single wavelength or the full spectral scans during this denaturation process provides thermodynamic information such as the melting temperature ...
The metallothioneins (MT) are a class of low molecular weight, cysteine rich proteins found in almost all living things. The high sulfhydryl content imparts to these proteins the unique ability to bind a wide range of metals. The metallothioneins have been implicated in the storage and transport of essential metals, such as zinc and copper, and in the detoxification of toxic metals, such as cadmium. However, the exact physiological functions of these proteins remain a matter of speculation. In particular, little is known about the interactions of copper with MT. In this thesis, the structural and mechanistic properties of copper binding to metallothionein is investigated using the spectroscopic techniques of circular dichroism, luminescence, and to a lesser extent, UV-visible absorption spectroscopy. These spectroscopic results are interpreted in terms of the Cu(I):MT stoichiometries at which distinct spectral characteristics are observed, the coordination geometry of the bound Cu(I) atoms, and the
1965, 9, 401. Deeth, R. ; Foulis, D. L. Phys. Chem. Chem. Phys. 2002, 4, 4292-4297. ; Hambley, T. ; Hitchman, M. ; Stratemeier, H. Inorg. Chem. 1995, 34, 3903-3911. Deeth, R. ; Gerloch, M. Inorg. Chem. 1984, 23, 3846-3853. Deeth, R. ; Anastasi, A. ; Wilcockson, M. J. J. Am. Chem. Soc. 2010, 132, 6876-6877. ); Montreal: Montreal, 2007 Deeth, R. J. Faraday Discuss. 2003, 124, 379-391. Deeth, R. ; Hearnshaw, L. J. A. Dalton Trans. 2006, 1092-1100. Deeth, R. J. Inorg. Chem. 2007, 46, 4492-4503. Norrby, P. CALCULATION OF MAGNETIC CIRCULAR DICHROISM SPECTRA WITH TIME-DEPENDENT DENSITY FUNCTIONAL THEORY MICHAEL SETH and TOM ZIEGLER Department of Chemistry, University of Calgary, 2500 University Dr NW, Calgary, AB T2N 1N4, Canada I. Introduction II. Theory A. MCD Spectroscopy B. Time-Dependent Density Functional Theory C. MCD with TDDFT III. Applications A. Small Molecules B. Porphyrins C. Metallonzymes IV. Concluding Remarks List of Symbols Acknowledgments References I. 41 47 47 58 61 74 74 87 93 100 ...
TY - JOUR. T1 - Classification of CD and absorption spectra in the Soret band of H2TMPyP bound to various synthetic polynucleotides. AU - Lee, Soomin. AU - Jeon, Sun Hee. AU - Kim, Bok Jo. AU - Han, Sung Wook. AU - Jang, Ho G.. AU - Kim, Seog K.. N1 - Funding Information: This research was supported by Korea Science and Engineering Foundation. (Grant no. 2000-1-12200-001-3.) PY - 2001/9/30. Y1 - 2001/9/30. N2 - The binding mode of porphyrins, namely meso-tetrakis(N-methyl pyridinium-4-yl)porphyrin (H2TMPyP), was classified in this work by absorption and circular dichroism(CD) spectroscopy. The three binding modes of intercalation, minor groove binding and external stacking exhibit their own characteristic absorption and CD spectra. Intercalation occurs for this porphyrin when bound to GC-rich polynucleotides at a low mixing ratio, as expected. This binding mode produces hypochromism and a red shift in the absorption band and a negative CD band in the Soret absorption region. When it is complexed ...
Valence Band Circular Dichroism in non-magnetic Ag-Ru0001 at normal emission. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Nearly all circular dichroism (CD) and linear dichroism (LD) spectrometers use a photoelastic modulator (PEM) in which an optical element is stressed using a high-tension voltage (HT) signal to induce birefringence. The birefringence consequently produces a phase difference between perpendicular polarization states of light passing through the PEM that is appropriate to CD or LD measurements. However, even without external stress (i.e., at zero HT) the PEM exhibits an inherent static birefringence. This article discusses the characterization of the static birefringence inherent to a PEM and its effect on the measurement of CD and LD, as well as the development and implementation of a novel model that accommodates for the presence of static birefringence. The model is validated with CD and LD experiments using purely chiral or linearly structured molecules (camphorsulfonic acid and chrysazin).. PDF Article ...
TY - JOUR. T1 - Protein Fiber Linear Dichroism for Structure Determination and Kinetics in A Low-Volume, Low-Wavelength Couette Flow Cell. AU - Dafforn, Timothy R.. AU - Rajendra, Jacindra. AU - Halsall, David J.. AU - Serpell, Louise C.. AU - Rodger, Alison. PY - 2004/1. Y1 - 2004/1. N2 - High-resolution structure determination of soluble globular proteins relies heavily on x-ray crystallography techniques. Such an approach is often ineffective for investigations into the structure of fibrous proteins as these proteins generally do not crystallize. Thus investigations into fibrous protein structure have relied on less direct methods such as x-ray fiber diffraction and circular dichroism. Ultraviolet linear dichroism has the potential to provide additional information on the structure of such biomolecular systems. However, existing systems are not optimized for the requirements of fibrous proteins. We have designed and built a low-volume (200 μL), low-wavelength (down to 180 nm), low-pathlength ...
By Knee, Kelly M Dixit, Surjit B; Aitken, Colin Echeverria; Ponomarev, Sergei; Beveridge, D L; Mukerji, Ishita ABSTRACT The A-to-B form transition has been examined in three DNA duplexes, d(CGCGAATTCGCG)^sub 2^, d(CGCGAATTGCGC), and d(CGCAAATTTCGC), using circular dichroism spectroscopy, ultraviolet resonance Raman (UVRR) spectroscopy, and molecular dynamics (MD) simulation. Circular dichroism spectra confirm that these molecules adopt the A form under conditions of reduced water activity. UVRR results, obtained under similar conditions, suggest that the transition involves a series of intermediate forms between A and B. Cooperative and distinct transitions were observed for the bases and the sugars. Independent MD simulations on d(CGCGAATTCGCG)^sub 2^ show a spontaneous change from the A to B form in aqueous solution and describe a kinetic model that agrees well with UVRR results. Based on these observations, we predict that the mechanism of the transition involves a series of A/B hybrid forms ...
Soft x-ray absorption spectroscopy and magnetic circular dichroism at the Co L{sub 2,3} edge have been applied to explore the near-interfacial magnetism of Co electrodes in Co/alumina based magnetic tunnel junctions. By taking into account the formation of CoO at the FM/barrier interface, the change in the total magnetic moment on metallic Co atoms as a function of barrier oxidation has been determined. The results demonstrate a strong correlation between the Co moments and measured TMR values, and an enhancement in the Co moments for moderate oxidation times.
10−2. This happens in paramagnetic systems that are at lower temperature or that have sharp lines in the spectroscopy. In biology, metalloproteins are the most likely candidates for MCD measurements, as the presence of metals with degenerate energy levels leads to strong MCD signals. In the case of ferric heme proteins, MCD is capable of determining both oxidation and spin state to a remarkably exquisite degree. In regular proteins, MCD is capable of stoichiometrically measuring the tryptophan content of proteins, assuming there are no other competing absorbers in the spectroscopic system. In addition, the application of MCD spectroscopy greatly improved the level of understanding in the ferrous non-heme systems because of the direct observation of the d-d transitions, which generally can not be obtained in optical absorption spectroscopy owing to the weak extinction coefficients and are often electron paramagnetic resonance silent due to relatively large ground-state sublevel splittings and ...
Supplementary MaterialsDocument S1. 25). Samples for NMR experiments were purified as previously described (8, 25). D2O for HX studies (99.96%) was obtained from Cambridge Isotope Laboratories (Tewksbury, MA). Urea (molecular biology grade, 99% pure) was obtained from Fisher Scientific (Pittsburgh, PA).?The concentrations of all urea solutions were determined by refractometry (26). Urea denaturation by circular dichroism Circular dichroism (CD) experiments were performed using a Pi-Star 180?spectropolarimeter from Applied Photophysics (Leatherhead, UK). I-domain solutions were diluted to a final protein concentration of 0.2?mg/mL in 20?mM sodium phosphate buffer (pH 6.0) and mixed using a Microlab 50 Titrator (Hamilton, Franklin, MA) to final urea concentrations ranging from 0 to 6 M. Samples were incubated for 3?h at 25C, and the CD signal was averaged for 20 scans at 220?nm using a 4-nm slit width and a 2.0-mm-pathlength cell. =?is?the baseline noise of the spectrum. Fits were done with the ...
Marker bands for specific secondary structures are most typically observed in the UV region below 260 nm and as far into the far-UV region as possible. It has been widely reported that CD measurements using synchroton radiation have been used to successfully measure spectra down to around 140 nm. As an alternative to synchrotron radiation, the J-1500 CD spectrometer has been improved for measurements in the vacuum UV region. ...
|p|The first transition metal iodato peroxido complex, K3[V2O2(O2)4(IO3)]·H2O (I), was prepared by crystallization from the KVO3 - KIO3 - H2O2 - H2O - ethanol (HNO3) solution. The dinuclear anion is immediately decomposed in aqueous solution; the 51V NMR spectrum exhibits signals corresponding to [VO(O2)2(H2O)]−, [V2O2(OH)(O2)4]3− and H2VO4− species only. The IR and Raman spectra contain all characteristic bands of the VO(O2)2 group and the coordinated IO3− ligand. Based on the positions of bands assigned to the vibrations of the VO(O2)2 groups a pentagonal pyramidal arrangement around the vanadium atoms can be supposed. The crystal structure was solved from X-ray synchrotron powder data by direct space method and refined by energy minimization in the solid state employing a hybrid PBE0 functional. This crystal and molecular structure, has confirmed the presence of hexacoordinated vanadium atoms and revealed asymmetric dinuclear structure of the [V2O2(O2)4(IO3)]3− ion
Erythrocyte ankyrin contains an 89-kDa domain (residues 2-827) comprised almost entirely of 22 tandem repeats of 33 amino acids which are responsible for the high affinity interaction of ankyrin with the anion exchanger (Davis, L., and Bennett, V. (1990) J. Biol. Chem. 265, 10589-10596). The question of whether the repeats are equivalent with respect to binding to the anion exchanger was addressed using defined regions of erythrocyte and brain ankyrins expressed in bacteria. The conclusion is that the repeats are not interchangeable and that the 44 residues from 722 to 765 are essential for high affinity binding between erythrocyte ankyrin and the anion exchanger. Residues 348-765 were active whereas a polypeptide of the same size (residues 305-721) but missing the 44 residues was not active. The difference between the active and inactive polypeptides was not caused by the degree of folding based on circular dichroism spectra. The 44 residues from 722 to 765 were not sufficient for binding since ...
Erythrocyte ankyrin contains an 89-kDa domain (residues 2-827) comprised almost entirely of 22 tandem repeats of 33 amino acids which are responsible for the high affinity interaction of ankyrin with the anion exchanger (Davis, L., and Bennett, V. (1990) J. Biol. Chem. 265, 10589-10596). The question of whether the repeats are equivalent with respect to binding to the anion exchanger was addressed using defined regions of erythrocyte and brain ankyrins expressed in bacteria. The conclusion is that the repeats are not interchangeable and that the 44 residues from 722 to 765 are essential for high affinity binding between erythrocyte ankyrin and the anion exchanger. Residues 348-765 were active whereas a polypeptide of the same size (residues 305-721) but missing the 44 residues was not active. The difference between the active and inactive polypeptides was not caused by the degree of folding based on circular dichroism spectra. The 44 residues from 722 to 765 were not sufficient for binding since ...
This is a rapid publication site that replaces Virtual Grand Rounds in Dermatology (vgrd.org). Please join and feel free to post cases. You can share the URL with friends. Since 2000, VGRD has been a valuable means to share cases in real time from ones home or office. AND GLADLY WOLDE HE LERNE AND GLADLY TECHE has served as an enduring and inspirational motto. For more information, see the About Page.. ...
BioAssay record AID 704382 submitted by ChEMBL: Binding affinity to salmon testes DNA at drug/DNA ratio of 0.03 by linear dichroism analysis.
Circular Dichroism (CD) Spectroscopy. Applied Photophysics Ltd., 2011. Retrieved 15 August 2013. Weisstein, Eric W. " ... Cuvettes to be used in circular dichroism experiments should never be mechanically stressed, as the stress will induce ... A cuvette (French: cuvette = "little vessel") is a small tube-like container with straight sides and a circular or square cross ...
Berova, Nina (2007). "Application of electronic circular dichroism in configurational and conformational analysis of organic ... Her publications include; Berova, Nina (2000). Circular Dichroism: Principles and Applications. Wiley. ISBN 9780471330035. ... Circular Dichroism and Raman Optical Activity. She was elected an honorary member of the Italian Chemical Society at the Scuola ... "Application of electronic circular dichroism in the study of supramolecular systems". Chemical Society Reviews. 43 (15): 5211- ...
2000). "Vibrational Raman Optical Activity". Circular dichroism: principles and applications. Wiley-VCH. ISBN 978-0-471-33003-5 ...
Berova, Nina (2000). Circular dichroism : principles and applications. New York, NY: Wiley-VCH. pp. 43-44. ISBN 0471330035. ...
Analysis of circular dichroism spectra". J Biol Chem. American Society for Biochemistry and Molecular Biology. 225 (4): 1075-93 ...
A Circular Dichroism and Permeability Study". Biochemistry. 22 (12): 2939-2944. doi:10.1021/bi00281a024. PMID 6871175. Drew, R ...
Photoelasticity Circular dichroism phase modulator for ellipsometry. ...
She worked on circular dichroism and bioseparation. In 1967 Phillips was the first African-American woman to complete a ... She developed circular dichroism and began to work on antibiotics and herbicides. At Dow Chemical Company Phillips helped ... She worked on the R17 virus, using circular dichroism and electron paramagnetic resonance to understand the conformation of the ...
These phenomena of specular circular birefringence and specular circular dichroism are jointly known as specular optical ... Circular conversion dichroism with almost ideal efficiency has been achieved in metamaterial-based chiral mirrors. In contrast ... Strong circular conversion dichroism due to extrinsic 2d chirality has been observed in metamaterials. Electromagnetic waves ... This phenomenon is known as circular dichroism and described by different imaginary parts of refractive indices for left- and ...
"Valley-selective circular dichroism of monolayer molybdenum disulphide". Nature Communications. 3 (6): 887. arXiv:1112.4013. ... The interband transitions in the vicinity of the K+ (or K-) point are coupled to right (or left) circular photon polarization ... Moreover, crystal symmetry leads to valley dependent optical selection rules: a right circular polarized photon (σ+) ... initializes a carrier in the K+ valley and a left circular polarized photon (σ-) initializes a carrier in the K- valley. Thanks ...
He also studied the fine structure of these chromophores using circular dichroism. More recently, he participated in a research ... "The circular dichroism of tyrosyl and tryptophanyl diketopiperazines". J. Biol. Chem. 243 (18): 4799-4805. PMID 5687722.CS1 ...
In regions of absorption, magnetic circular dichroism is observable. Circular dichroism Magnetic circular dichroism Absorption ... This unequal absorption is known as circular dichroism. Circular dichroism causes incident linearly polarized light to become ... In order for a molecule (or crystal) to exhibit circular birefringence and circular dichroism, it must be distinguishable from ... These properties account for the fact that optical rotatory dispersion and circular dichroism are widely used in organic and ...
Circular dichroism spectrometers contain a monochromator, for example. Lasers produce light which is much more monochromatic ...
Circular dichroism, fluorescence, and ultraviolet difference spectroscopic studies". The Journal of Biological Chemistry. 267 ( ...
"The circular dichroism of an optically active benzene chromophore :: University of Southern California Dissertations and Theses ... Schnepp, O.; Allen, Susan; Pearson, Earl F. (1970-08-01). "The Measurement of Circular Dichroism in the Vacuum Ultraviolet". ... Her PhD dissertation research used circularly polarized light, specifically circular dichroism, to investigate electronic ... "The circular dichroism of an optically active benzene chromophore :: University of Southern California Dissertations and Theses ...
Allendorf, M D; Spira, D J; Solomon, E I (1985). "Low-temperature magnetic circular dichroism studies of native laccase: ... He is an expert in magnetic circular dichroism spectroscopy. Alfred P. Sloan Foundation Fellow, 1976-79 Dupont and General ...
His group was responsible for the discovery of Electron magnetic circular dichroism in 2006. Peter Schattschneider completed ... "Detection of magnetic circular dichroism using a transmission electron microscope". Nature. 441 (7092): 486-488. Bibcode: ... EDFC, Passau 1995 (with Karlheinz Steinmüller) Linear and chiral dichroism in the electron microscope. Pan Stanford, Singapore ...
Mammana, A; Carroll, GT; Feringa, BL (2012). "Circular Dichroism of Dynamic Systems: Switching Molecular and Supramolecular ... Angewandte and the main textbook in the field of circular dichroism, Comprehensive Chiroptical Spectroscopy. He won the Theodor ... "Dynamic Control and Amplification of Molecular Chirality by Circular Polarized Light". Science. American Association for the ...
Heindl MC, Fermandjian S, Keil B (July 1980). "Circular dichroism comparative studies of two bacterial collagenases and ...
Circular dichroism is one of the most general and basic tools to study protein folding. Circular dichroism spectroscopy ... Similar to circular dichroism, the stimulus for folding can be a denaturant or temperature. The study of protein folding has ... As for fluorescence spectroscopy, circular-dichroism spectroscopy can be combined with fast-mixing devices such as stopped flow ... The more recent developments of vibrational circular dichroism (VCD) techniques for proteins, currently involving Fourier ...
General secondary structure composition can be determined via circular dichroism. Vibrational spectroscopy can also be used to ...
Gruenewald B, Knoche W (1978). "Pressure jump method with detection of optical-rotation and circular-dichroism". Review of ...
A perfect chiral mirror exhibits circular conversion dichroism with ideal efficiency. Chiral mirrors can be realized by placing ... In the presence of losses, extrinsic planar chirality can result in circular conversion dichroism, as described above. ... opposite propagation directions of the incident wave and therefore the effect is referred to as circular conversion dichroism. ... The asymmetric transmission phenomenon arises from different, e.g. left-to-right, circular polarization conversion efficiencies ...
The denaturation can be monitored through a change in circular dichroism. A reduction in melting temperature indicates a ...
Optical rotatory dispersion Protein circular dichroism data bank Two-photon circular dichroism Vibrational circular dichroism ... Linear dichroism Magnetic circular dichroism Optical activity Optical isomerism Optical rotation ... Petralli-Mallow, T.T.; Wong, T.M.; Byers, J.D.; Yee, H.I.; Hicks, J.M. (1993). "Circular dichroism spectroscopy at interfaces: ... By contrast, other nonlinear scattering effects, such as two-photon circular dichroism and hyper-Raman are non-parametric - ...
"Atomic scale imaging of magnetic circular dichroism by achromatic electron microscopy". Nature Materials. 17 (3): 221-225. ...
2017 Circular Dichroism and Linear Dichroism 2014 Molecular Geometry 2010 Linear Dichroism and Circular Dichroism: A Textbook ... "Linear Dichroism and Circular Dichroism av Alison Rodger, Tim Dafforn, Bengt Norden (Bok)". Bokus.com (in Swedish). Retrieved ... Nordén, Bengt; Rodger, Alison; Dafforn, Tim (2010). Linear Dichroism and Circular Dichroism: A Textbook on Polarized-light ... Circular Dichroism and Linear Dichroism. Oxford University Press. ISBN 9780198558972. Marrington, R.; Dafforn, T. R.; Halsall, ...
Pescitelli G, Woody RW (2012). "The Exciton Origin of the Visible Circular Dichroism Spectrum of Bacteriorhodopsin". Journal of ...
Circular birefringence and circular dichroism are the manifestations of optical activity. Optical activity occurs only in ... These phenomena of specular circular birefringence and specular circular dichroism are jointly known as specular optical ... Cryptochirality Specific rotation Circular dichroism Birefringence Geometric phase Polarization Levorotation and dextrorotation ... The difference in the refractive indices between the two circular polarizations quantifies the strength of the circular ...
X-ray diffraction, circular dichroism, nuclear magnetic resonance and ab initio studies". Tetrahedron. 53 (17): 6113-6144. doi: ...
... but when measured carefully with X-ray magnetic circular dichroism, has an extremely weak paramagnetic contribution that is ... "Measurement of a Pauli and Orbital Paramagnetic State in Bulk Gold Using X-Ray Magnetic Circular Dichroism Spectroscopy". ...
... circular dichroism, and dual polarisation interferometry. Computer simulations of the dynamic behavior of receptors have been ...
Circular dichroism spectroscopy. *Coherent anti-Stokes Raman spectroscopy. *Cold vapour atomic fluorescence spectroscopy ...
Circular dichroism shows that normal PrPC has 43% alpha helical and 3% beta sheet content, whereas PrPSc is only 30% alpha ...
Circular dichroism is another laboratory technique for determining internal β-sheet / α-helical composition of proteins. ...
Circular dichroism. *Dual-polarization interferometry. *Absorbance. *Fluorescence. *Fluorescence anisotropy. Translational ...
"Structural and circular-dichroism studies on the interaction between human C1-esterase inhibitor and C1s". The Biochemical ...
Significant examples include 193 nm photolithography equipment (for semiconductor manufacturing) and circular dichroism ...
Just as an ocean wave striking a lighthouse produces secondary circular waves emanating from the lighthouse, so an X-ray ...
"Applied Circular Dichroism: A Facile Spectroscopic Tool for Configurational Assignment and Determination of Enantiopurity". ...
Circular dichroism. *Dual-polarization interferometry. *Absorbance. *Fluorescence. *Fluorescence anisotropy. Translational ...
Circular dichroism. *Dual-polarization interferometry. *Absorbance. *Fluorescence. *Fluorescence anisotropy. Translational ...
Circular dichroism. *Nuclear magnetic resonance. *Mass spectrometry. *Atomic force microscope. *Paramagnetic probes ...
... consisting of circular dichroism, Fourier transform infrared, and nuclear magnetic resonance spectroscopy. These models are ...
... of surface chemistry and solution concentration on the conformation of adsorbed proteins using an improved circular dichroism ...
... enantiomer based on vibrational and electronic circular dichroism spectroscopy and X-ray crystallography. An (S)-configuration ...
... circular dichroism experiments suggest that Alphabodies can refold correctly after being denatured. These properties allow ...
Circular dichroism spectroscopy. *Coherent anti-Stokes Raman spectroscopy. *Cold vapour atomic fluorescence spectroscopy ...
Circular dichroism is another laboratory technique for determining internal β-sheet / α-helical composition of proteins. ...
The far-UV (170-250 nm) circular dichroism spectrum of helices is also idiosyncratic, exhibiting a pronounced double minimum at ... Either circular style of diagram can provide an intuitive and easily visualizable 2D picture that characterizes the disposition ... Two of these emphasize circular placement around the cylindrical cross-section: The first-developed such diagram is called the ...
Circular dichroism spectroscopy. *Coherent anti-Stokes Raman spectroscopy. *Cold vapour atomic fluorescence spectroscopy ... Such an aperture is called a Jacquinot stop.[1] For a given resolution and wavelength this circular aperture allows more light ...
... helix structure in the crystalline state is derived from X-ray crystallography and from NMR spectroscopy and circular dichroism ...
... recent circular dichroism (CD) experiments have shown osmolyte to act through an enthalpic effect.[46] The molecular mechanism ...
Circular birefringence and circular dichroism are the manifestations of optical activity. Optical activity occurs only in ... These phenomena of specular circular birefringence and specular circular dichroism are jointly known as specular optical ... The difference in the refractive indices between the two circular polarizations quantifies the strength of the circular ... ORD spectra and circular dichroism spectra are related through the Kramers-Kronig relations. Complete knowledge of one spectrum ...
Significant examples include 193 nm photolithography equipment (for semiconductor manufacturing) and circular dichroism ...
... of surface chemistry and solution concentration on the conformation of adsorbed proteins using an improved circular dichroism ...
Protein conformation in cell membrane preparations as studied by optical rotatory dispersion and circular dichroism. ...
Circular dichroism (CD) is then defined as Δk = k- - k+, and it follows the sign convention of natural optical activity. In the ... Magnetic circular dichroism (MCD) is the differential absorption of left and right circularly polarized (LCP and RCP) light, ... Upton, A. H. P.; Williamson, B. E. (1994). "Magnetic circular dichroism and absorption spectra of hexacyanoferrate(III) in a ... In this system, ω = 2 π ν {\displaystyle \omega =2\pi \nu } is the circular frequency, and n ~ {\displaystyle {\tilde {n}}} = n ...
... light scattering Small angle scattering Ultrafast laser spectroscopy Dual-polarization interferometry and circular dichroism ...
Circular dichroism (CD) is then defined as Δk = k- - k+, and it follows the sign convention of natural optical activity. In the ... Magnetic circular dichroism (MCD) is the differential absorption of left and right circularly polarized (LCP and RCP) light, ... Upton, A. H. P.; Williamson, B. E. (1994). "Magnetic circular dichroism and absorption spectra of hexacyanoferrate(III) in a ... In this system, ω = 2 π ν {\displaystyle \omega =2\pi \nu } is the circular frequency, and n ~ {\displaystyle {\tilde {n}}} = n ...
circular dichroism (uncountable). *(chemistry, physics) the differential absorption of left-handed and right-handed circularly ... Retrieved from "https://en.wiktionary.org/w/index.php?title=circular_dichroism&oldid=44451934" ...
Protein backbone circular dichroism Protein circular dichroism (CD) spectroscopy is usually divided into (1) far UV or backbone ... Linear dichroism and circular dichroism: a textbook on polarized spectroscopy. Cambridge: Royal Society of Chemistry; 2010. ... Rodger A, Nordén B. Circular dichroism and linear dichroism. Oxford: Oxford University Press; 1997.Google Scholar ... Single peptide bonds exhibit poly(pro)II ("random coil") circular dichroism spectra. J Am Chem Soc. 2005;127:9700-1.PubMed ...
Circular Dichroism. The Aviv Circular Dichroism Spectrometer, model 215, records CD as a function of wavelength, time, ...
Optical rotatory dispersion Protein circular dichroism data bank Two-photon circular dichroism Vibrational circular dichroism ... Molar ellipticity is circular dichroism corrected for concentration. Molar circular dichroism and molar ellipticity, [θ], are ... Optical rotary dispersion and circular dichroism share the same quantum information content. Linear dichroism Magnetic circular ... is the molar circular dichroism. This intrinsic property is what is usually meant by the circular dichroism of the substance. ...
"Vibrational Circular Dichroism of Polypeptides XII. Re-evaluation of the Fourier Transform Vibrational Circular Dichroism of ... acid Birefringence Circular dichroism Density functional theory DNA DNA structure IR spectroscopy Magnetic circular dichroism ... 1988). "Vibrational Circular Dichroism of Biopolymers". In E. D. Schmid; F. W. Schneider; F. Siebert (eds.). Spectroscopy of ... Vibrational circular dichroism (VCD) is a spectroscopic technique which detects differences in attenuation of left and right ...
Circular Dichroism (CD) analysis from SGS - meet regulatory requirements with class-leading CD spectroscopic analysis, alpha ... Life Sciences Circular Dichroism Analysis. Circular Dichroism (CD) analysis from SGS - meet regulatory requirements with class- ... Trusted circular dichroism from the leading provider. As the worlds leading provider of circular dichroism, we provide the ... Home › Life Sciences › Laboratory Services › Protein and Peptide Analysis › Circular Dichroism Analysis ...
... electronic circular dichroism), magnetic circular dichroisms (magnetic vibrational circular dichroism, x-ray magnetic circular ... dichroism), fluorescence detected circular dichroism, near-infrared circular dichroism, vibrational circular dichroism, Fourier ... transform infrared circular dichroism, high pressure liquid chromatography circular dichroism, stopped-flow circular dichroism ... This paper reviews the best known techniques using circular dichroism spectroscopy such as conventional circular dichroism (i.e ...
... (CD) is a form of spectroscopy based on the differential absorption of left- and right- ... is the molar circular dichroism. This is what is usually meant by the circular dichroism of the substance. Although ΔA is ... Circular dichroism (CD) is a form of spectroscopy based on the differential absorption of left- and right-handed circularly ... Generally, the circular dichroism effect is small, so tanθ is small and can be approximated as θ in radians. Since the ...
A stopped-flow circular dichroism instrument, with a total accessible wavelength range of 200 to 750 nanometers, has been ... Results obtained by following two such reactions at 222 nanometers represent direct monitoring by circular dichroism of rapid ...
Circular dichroism (CD) is being increasingly recognised as a valuable technique for examining the structure of proteins in ... How to study proteins by circular dichroism Biochim Biophys Acta. 2005 Aug 10;1751(2):119-39. doi: 10.1016/j.bbapap.2005.06.005 ... Circular dichroism (CD) is being increasingly recognised as a valuable technique for examining the structure of proteins in ...
... allows us to use light to investigate the structural properties of biological molecules such as ... Circular Dichroism Spectroscopy allows us to use light to investigate the structural properties of biological molecules such as ... Circular Dichroism Spectroscopy Biophysical Analysis Circular Dichroism Spectroscopy Circular Dichroism Spectroscopy Isothermal ...
... and circular dichroism (CD) was induced at a low temperature. The CD induction upon cooling was a rather slow process, ... and circular dichroism (CD) was induced at a low temperature. The polymer concentration of the minor separating phase, ... 2 Circular dichroism (CD), optical activity and circular polarized luminescence are additional optical and electro-optical ... Induced circular dichroism of an optically active polyfluorene derivative in phase-separating solutions. *Yusuke Sanada1. & ...
Circular dichroism (CD) is a powerful chiro-optical spectroscopic technique for elucidating macromolecular structure of ... Circular dichroism spectroscopy is a technique where the difference in the absorption of left and right circularly polarized ... Circular dichroism spectroscopy (CD) is an essential analytical technique used to analyze chirality in molecules through their ... Circular Dichroism spectroscopy exploits the fundamental property described by the Cotton Effect. See here the Wikipedia ...
Circular Dichroism. Circular Dichroism. Circular dichroism (CD) refers to the differential absorption of left and right ... Circular Polarization - Circular Dichroism. ... Circular dichroism (CD) is the differential absorption of left- and right- ... Circular Dichroism. ... Circular dichroism is one of the most general and basic tools to study protein folding ... Circular ... Other articles related to circular dichroism:. Circular Dichroism - Experimental Limitations. ... At the quantum mechanical ...
Johnson WC (1985) Circular dichroism and its empirical application to biopolymers. Methods Biochem Anal 31:61-163PubMed ... Kelly SM, Jess TJ, Price NC (2005) How to study proteins by circular dichroism. Biochim Biophys Acta 1751(2):119-139PubMed ... Circular dichroism Ligand binding Titration Binding constant UV denaturation Protein stability Data processing ... Brown EM, Groves ML (1985) Effect of temperature on the circular dichroism spectra of-β2-microglobulins. FEBS Lett 184(1):36-39 ...
Scharnagl, C.; Köst-Reyes, Eliana; Schneider, Siegfried; Köst, Hans-Peter; Scheer, Hugo (1983): Circular dichroism of ...
Accepted Manuscript: Circular dichroism in photoelectron images from aligned nitric oxide molecules ... The results demonstrate that circular dichroism is observed even when cylindrical symmetry is maintained, and serve as a ... We have used velocity map photoelectron imaging to study circular dichroism of the photoelectron angular distributions (PADs) ... reminder that dichroism is a general feature of the multiphoton ionization of atoms and molecules. Furthermore, the observed ...
Circular dichroism in ion yield is achieved by resonance-enhanced multiphoton ionization. The feasibility of circular dichroism ... Circular dichroism in ion yield has promising new potentials for chiral analysis. Our progress of its development is described ... Ab initio study of the one- and two-photon circular dichroism of R-(+)-3-methyl-cyclopentanone. Rizzo, Antonio; Na Lin; Ruud, ... The study explores in vitro by circular dichroism and mass spectrometry the effects of pH, Cu+2 ions and sheetbreakers on the ...
33.55.Ad Optical activity, optical rotation; circular dichroism ... 33.55.Ad Optical activity, optical rotation; circular dichroism ...
These useful tips and tricks will help you get the best out of any circular dichroism measurement. Contact us to request a free ... for J-1000 Series Circular Dichroism Spectrometers. Digital Integration Time (D.I.T.). Circular Dichroism Spectra (with data ... Circular Dichroism Tips & Tricks for Biological Samples. Getting the Best out of Your Circular Dichroism Analysis. Get a Poster ... Since JASCO circular dichroism spectrophotometers offer a continuous scan mode (and step-scan mode), the D.I.T. must be taken ...
The magnetic circular dichroism and absorption spectra of Ta atoms isolated in an Ar matrix have been observed in the range 220 ... MAGNETIC CIRCULAR DICHROISM OF MATRIX-ISOLATED TANTALUM ATOMS. Please use this identifier to cite or link to this item: http:// ...
The Chirascan qCD (CD 250TM/AP) is used for characterization of biomolecules (secondary structure of proteins, polysaccharides and nucleic acid), determination of absolute configuration, and stereo chemical analysis. A primary use is in analysing the structure or conformation of large biological macromolecules, particularly proteins and DNA. The detector is able to measure UV to NIR range 165-900nm. ...
X-ray magnetic circular dichroism (XMCD) is a powerful tool for the element-specific study of the magnetic structure of complex ... The idea of this work was to combine a bulk sensitive hard X-ray probe with the magnetic circular dichroism (MCD) sensitivity ... Top image: Magnetic circular dichroism (MCD) combined with resonant inelastic X-ray scattering (RIXS). ... Strong K-edge magnetic circular dichroism observed in photon-in/photon-out spectroscopy. ...
Circular dichroism (CD) spectroscopy is an optical technique that measures the difference in the absorption of left and right ... Maestre, M.F. Circular dichroism of DNA films: Reversibility studies. J. Mol. Biol 1970, 52, 543-556. [Google Scholar] ... Conformational Changes in DNA upon Ligand Binding Monitored by Circular Dichroism. Yu-Ming Chang 1, Cammy K.-M. Chen 1 and Ming ... Circular dichroism was also used to measure the interactions between hairpin DNA duplexes that contains regions of 2 bp G-tract ...
... Tengstedt, Carl Linköping University, ... X-ray magnetic circular dichroism measurements recorded at the V L2,3 edge confirmed room temperature magnetic ordering. A ...
... Bertucci C., Cimitan S ... Circular dichroism experiments supported this hypothesis. Furthermore, taxanes binding to the serum carrier was characterized ... drug paclitaxel and of several structural analogues has been characterized by bioaffinity chromatography and circular dichroism ...
Circular dichroism (CD) spectrometers market comprises an elaborate executive summary, which includes a market snapshot with ... circular dichroism spectrometers market was valued at approximately US$ 28.0 Mn in 2016 and is anticipated to register a CAGR ... DichroWeb and Protein Circular Dichroism Data Bank (PCDDB) are the two noteworthy reference information hotspots for circular ... The global circular dichroism spectrometers market is foreseen to expand at a CAGR of over 6.0% within the forecast period from ...
  • Protein circular dichroism (CD) spectroscopy is usually divided into (1) far UV or backbone (meaning the amide transitions, Fig. 1 ) with data collected from ~190 to 250 nm and (2) near UV or aromatic with data collected from 250 to 300 nm. (springer.com)
  • Linear dichroism and circular dichroism: a textbook on polarized spectroscopy. (springer.com)
  • Modern techniques for circular dichroism spectroscopy. (springer.com)
  • It is the extension of circular dichroism spectroscopy into the infrared and near infrared ranges. (wikipedia.org)
  • This paper reviews the best known techniques using circular dichroism spectroscopy such as conventional circular dichroism (i.e. electronic circular dichroism), magnetic circular dichroisms (magnetic vibrational circular dichroism, x-ray magnetic circular dichroism), fluorescence detected circular dichroism, near-infrared circular dichroism, vibrational circular dichroism, Fourier transform infrared circular dichroism, high pressure liquid chromatography circular dichroism, stopped-flow circular dichroism, and synchrotron radiation circular dichroism. (nih.gov)
  • Also, we have described here the most important applications of circular dichroism spectroscopy in structural biochemistry and nanoscience. (nih.gov)
  • Circular dichroism (CD) is a form of spectroscopy based on the differential absorption of left- and right-handed circularly polarized light. (bionity.com)
  • Circular Dichroism Spectroscopy allows us to use light to investigate the structural properties of biological molecules such as proteins, DNA and RNA. (jic.ac.uk)
  • Circular dichroism spectroscopy (CD) is an essential analytical technique used to analyze chirality in molecules through their optical activity. (jascoinc.com)
  • Circular dichroism spectroscopy is a technique where the difference in the absorption of left and right circularly polarized light in optically active substances is measured. (jascoinc.com)
  • The feasibility of circular dichroism spectroscopy and quantitative determination of circular dichroism by this method is demonstrated. (ebscohost.com)
  • Circular dichroism (CD) spectroscopy is an optical technique that measures the difference in the absorption of left and right circularly polarized light. (mdpi.com)
  • Researchers from different sectors are indulging into various activities to collect reference information for circular dichroism of different biomolecules which is prognosticated to draw in new scientists to receive CD spectroscopy as a favored analytical tool. (sbwire.com)
  • The thermal unfolding of xylanase A from Streptomyces lividans, and of its isolated substrate binding and catalytic domains, was studied by differential scanning calorimetry and Fourier transform infrared and circular dichroism spectroscopy. (nih.gov)
  • In the present study, near-infrared magnetic circular dichroism spectroscopy is employed to investigate the nature of Fe( II ) coordination at the two transition-metal-binding sites of CP that are a His 3 Asp motif (site 1) and a His 6 motif (site 2). (rsc.org)
  • In this study we show that the highly sensitive method of synchrotron radiation circular dichroism (SRCD) spectroscopy can be used as a powerful tool to investigate the structure of the extramembranous C-terminal domain (CTD) of the prokaryotic voltage-gated sodium channel (Na V ) from Bacillus halodurans , NaChBac. (pnas.org)
  • The process from a viscous isotropic solution to a stable gel was monitored by vibrational circular dichroism (VCD) spectroscopy. (rsc.org)
  • Circular dichroism (CD) (and synchrotron circular dichroism (SCD)) spectroscopy is a rapid, highly sensitive technique used to investigate structural conformational changes in biomolecules in response to interactions with ligands in solution and in film. (mdpi.com)
  • To reveal the amount of some of these damages we applied circular dichroism and UV absorption spectroscopy. (novapublishers.com)
  • Synchrotron radiation circular dichroism (SRCD) spectroscopy was used to analyze the dynamic solution structure of the apoA-I protein in the apo- and HDL-states and the protein structure conversion in HDL formation. (lu.se)
  • The remarkable fingerprinting capabilities of the two-photon circular dichroism spectroscopy are highlighted. (diva-portal.org)
  • This is the first report of DNA stem-loops self-assembled by 'foot-loop' interactions into either two-dimensional strings or three-dimensional spirals, distinguished by circular dichroism spectroscopy. (scielo.org.za)
  • Circular dichroism (CD) spectroscopy of 1.5 µ M ml 1 DNA strand concentration in 100 mMl 1 sodium chloride, 5 mMl 1 magnesium chloride, and 10 mMl 1 sodium cacodylate, at pH 7.0 in a 1 ml jacketed quartz cuvette, was performed in a Jasco J-810 spectropolarimeter with ten accumulations per scan. (scielo.org.za)
  • Huang H, Liu X, Han R, Wang G (2017) Study on the Interaction of Retinoic Acids to Human Serum Albumin by Fluorescence and Circular Dichroism Spectroscopy. (omicsonline.org)
  • In this paper, the interactions between RAs and human serum albumin (HSA) were examined by steady state fluorescence, time-resolved fluorescence and circular dichroism spectroscopy (CD). (omicsonline.org)
  • In particular, little is known about the interactions of copper with MT. In this thesis, the structural and mechanistic properties of copper binding to metallothionein is investigated using the spectroscopic techniques of circular dichroism, luminescence, and to a lesser extent, UV-visible absorption spectroscopy. (uwo.ca)
  • Angewandte Chemie DOI: 10.1002/ange.201006255 H2 Activation Iron-Chromophore Circular Dichroism of [Fe]-Hydrogenase: The Conformational Change Required for H2 Activation** Seigo Shima,* Sonja Vogt, Andreas Gbels, and Eckhard Bill Circular dichroism (CD) spectroscopy is a very sensitive method used to detect changes in inherently chiral chromophores and in achiral chromophores embedded in chiral surroundings. (docme.ru)
  • The combined analysis of magnetization, 57Fe Mössbauer spectroscopy and Fe K-edge x-ray magnetic circular dichroism (XMCD) measurements suggests that the origin of the anomalous magnetic behavior found at xc=0.55 is mainly due to the Ho sublattice. (csic.es)
  • Polarization-dependent photon spectroscopy (dichroism) using signal-enhancing superchiral beams is shown to be sensitive to magnetic properties of the sample, whereas previous investigations explored charge-like electronic properties of chiral samples. (arxiv.org)
  • We go on to describe the use of circular dichroism spectroscopy and force spectroscopy with the atomic force microscope for the elucidation of structural and mechanical properties of these unusual helical species. (whiterose.ac.uk)
  • Lassen, PR 2005, ' Structure and Absolute Configuration of Nyasol and Hinokiresinol via Synthesis and Vibrational Circular Dichroism Spectroscopy ', Journal of Natural Products , vol. 68, no. 11, pp. 1603-1609. (dtu.dk)
  • The present study deals with an investigation of metal ion induced DNA condensation by means of Vibrational Circular Dichroism spectroscopy (VCD). (jbsdonline.com)
  • Julie M. DiNitto and John M. Kenney, "Modeling, Characterizing, and Accommodating Static Birefringence in Circular and Linear Dichroism Spectroscopy," Appl. (osapublishing.org)
  • The changes in the chiroptical properties of both 1(S) and 1(R) were used (by circular dichroism (CD) spectroscopy) to monitor the formation of these chiral self-assemblies in solution. (tcd.ie)
  • We have used circular dichroism and UV absorption spectroscopy to characterize the formation and melting behaviour of an intramolecular DNA triple helix containing parallel T*A:T and G*G:C triplets. (cnrs-orleans.fr)
  • For the first time, the success of a methodology for the determination of enantiomeric excess (% ee) in chiral solid samples by vibrational circular dichroism (VCD) spectroscopy is reported. (sparrho.com)
  • abstract = "The absolute configuration of the norlignan (+)-nyasol was determined to be S by comparison of the experimental vibrational circular dichroism data with first-principle calculations taking into account the eight lowest energy conformations. (dtu.dk)
  • Magnetic circular x-ray dichroism in Co/Pt multilayers (abstract)' Journal of Applied Physics. (nii.ac.jp)
  • Publications] T.Koide, N.Nakajima, T.Shidara, H.Miyauchi, H.Kawabe, H.Fukutani, A.Fujimori, K.Iio, T.Katayama and Y.Suzuki: 'Magnetic circular x-ray dichroism in Co/Pt multilayrs (abstract)' J.Appl.Phys.79. (nii.ac.jp)
  • Magnetic circular dichroism (MCD) is the differential absorption of left and right circularly polarized (LCP and RCP) light, induced in a sample by a strong magnetic field oriented parallel to the direction of light propagation. (wikipedia.org)
  • Circular dichroism (CD) is dichroism involving circularly polarized light, i.e., the differential absorption of left- and right-handed light. (wikipedia.org)
  • Vibrational circular dichroism (VCD) is a spectroscopic technique which detects differences in attenuation of left and right circularly polarized light passing through a sample. (wikipedia.org)
  • When either E R or E L is equal to zero (when there is complete absorbance of the circular polarized light in one direction), θ is 45° and the light is circularly polarized. (bionity.com)
  • In a circular dichroism spectrophotometer, linearly polarized light is created to later produce circularly polarized light. (jascoinc.com)
  • Circular dichroism (CD) refers to the differential absorption of left and right circularly polarized light. (primidi.com)
  • Electronic circular dichroism and circularly polarized luminescence acid/base switching activity has been demonstrated in helicene-bipyridine proligand 1 a and in its "rollover" cycloplatinated derivative 2 a. (archives-ouvertes.fr)
  • Circular dichroism, with units of millidegrees, reflects detailed helical geometry and is defined as the difference between the absorption of left- and right-handed, circularly-polarised light measured as a function of wavelength (nm). (scielo.org.za)
  • The circular dichroism of an optically active material is described by differential absorption of the left- and right-handed circularly polarized light. (techconnectworld.com)
  • The measurement of optical circular dichroism, i. e. the difference in absorption of left-hand and right-hand circularly polarized light by optically active compounds, is one of the most modern optical methods of investigation. (docme.ru)
  • Core-level magnetic circular dichroism measurements were made for the multilayrs using circularly polarized synchrotron radiation. (nii.ac.jp)
  • Enantiomers of the monoterpene limonene have been investigated by (2+1) resonance enhanced multiphoton ionization and photoelectron circular dichroism employing tuneable, circularly polarized femtosecond laser pulses. (nottingham.ac.uk)
  • Here, one of the challenges of interpreting electronic circular dichroism (ECD) band spectra is that different states may have different rotatory strength signs, determined by their absolute configuration. (osti.gov)
  • article{osti_1467568, title = {An atomic orbital based real-time time-dependent density functional theory for computing electronic circular dichroism band spectra}, author = {Goings, Joshua J. and Li, Xiaosong}, abstractNote = {Here, one of the challenges of interpreting electronic circular dichroism (ECD) band spectra is that different states may have different rotatory strength signs, determined by their absolute configuration. (osti.gov)
  • This chapter provides an overview of how to perform circular dichroism (CD) experiments, detailing methods, hints and tips for successful CD measurements. (springer.com)
  • The results of fluorescence measurements of magnetic circular dichroism (MCD) in Mn L 2,3 X-ray emission and absorption for Heusler alloys NiMnSb and Co 2 MnSb are presented. (diva-portal.org)
  • A convenient and reliable approach for the absolute configuration assignment of oxindole derivatives 2a?m by vibrational circular dichroism (VCD) measurements and evaluation of the VCD bisignated couplet resulting from the interaction of the C2 and C9 carbonyl groups is presented. (edu.mx)
  • The quantized circular dichroism revealed in this work designates depletion rate measurements as a universal probe for topological order in quantum matter. (sciencemag.org)
  • Porvair Sciences introduces new black Krystal UV Quartz bottomed microplates that offer excellent optical transmission in the wavelength range 185nm to 1100nm making them ideal for labs looking to make Circular Dichroism (CD) measurements. (technologynetworks.com)
  • Scientific examples showing X-ray magnetic circular and X-ray magnetic linear dichroism measurements are also presented. (epfl.ch)
  • Within the project we will develop a compact velocity-map imaging (VMI) spectrometer for femtosecond photoelectron circular dichroism (PECD) measurements. (laserlab-europe.eu)
  • Rodger A, Nordén B. Circular dichroism and linear dichroism. (springer.com)
  • Circular dichroism (CD) and linear dichroism (LD) are spectroscopic techniques that are becoming increasingly important probes of molecular structure, especially with proteins and DNAs. (oup.com)
  • Nearly all circular dichroism (CD) and linear dichroism (LD) spectrometers use a photoelastic modulator (PEM) in which an optical element is stressed using a high-tension voltage (HT) signal to induce birefringence. (osapublishing.org)
  • A stopped-flow circular dichroism instrument, with a total accessible wavelength range of 200 to 750 nanometers, has been constructed and provides a spectroscopic method for kinetic investigations of a wide array of fast reactions in which optical activity changes in absorbing regions are involved. (sciencemag.org)
  • Albany, NY -- ( SBWIRE ) -- 12/07/2018 -- The global circular dichroism (CD) spectrometer market is anticipated to grow due to presence of several regional and international players. (sbwire.com)
  • The report then estimates 2018-2023 market development trends of Vibrational Circular Dichroism Spectrometer industry. (marketstudyreport.com)
  • List Figure 2013-2018 Vibrational Circular Dichroism Spectrometer Capacity Production and Growth Rate Figure 2013-2018 Vibrational Circular Dichroism Spectrometer Production Global Market Share Figure Vibrational Circular Dichroism Spectrometer Product and Specifications Table 2013-2018 Vibrational Circular Dichroism Spectrometer Product Capacity, Production, and Production Value etc. (marketstudyreport.com)
  • The main part of the book (105 pp.) is devoted to the application of circular dichroism. (docme.ru)
  • Location and dynamics of tryptophan in transmembrane α-helix peptides: a fluorescence and circular dichroism study. (ebscohost.com)
  • The aim of this work was to evaluate the ability of oxidative and glycative stressors to modify properties of human serum albumin (HSA) by analyzing markers of glycation (pentosidine) and oxidation (advanced oxidative protein products (AOPPs)) and assessing fluorescence and circular dichroism. (mdpi.com)
  • The highly collimated micro-beam available at B23 beamline for synchrotron radiation circular dichroism (SRCD) at Diamond Light Source (DLS) offers many advantages to benchtop instruments. (springer.com)
  • The advantage of synchrotron radiation circular dichroism (SRCD) measured with synchrotron beamlines compared to the CD from benchtop instruments is the extended spectral far-UV region that increases the accuracy of secondary structure estimations, in particular under high ionic strength conditions. (springer.com)
  • Since Δ ϵ {\displaystyle \Delta \epsilon } is a function of wavelength, a molar circular dichroism value ( Δ ϵ {\displaystyle \Delta \epsilon } ) must specify the wavelength at which it is valid. (wikipedia.org)
  • The Aviv Circular Dichroism Spectrometer, model 215, records CD as a function of wavelength, time, temperature, pH and concentration using a double monochromator containing two UV grade prisms as dispersing elements. (niu.edu)
  • This ellipticity ( q ) as a function of wavelength defines a circular dichroism spectrum. (jascoinc.com)
  • Circular polarization occurs when the phase of an orthogonal electric field deviates by 1/4 wavelength. (jascoinc.com)
  • The chiral periodic structure in the scale of optical wavelength gives rise to strong and characteristic circular dichroism responses at visible wavelengths. (techconnectworld.com)
  • Differential scanning calorimetric, circular dichroism, and Fourier transform infrared spectroscopic characterization of the thermal unfolding of x. (nih.gov)
  • Fourier transform infrared circular dichroism spectrophotometer was developed for investigating the secondary structures of biopolymers such as proteins and nucleic acids. (nii.ac.jp)
  • Then Δ ϵ = ϵ L − ϵ R {\displaystyle \Delta \epsilon =\epsilon _{L}-\epsilon _{R}\,} is the molar circular dichroism. (wikipedia.org)
  • is the molar circular dichroism. (bionity.com)
  • Chiral molecules exhibit circular birefringence or optical rotation, where the velocity of the light passing through an optically active medium will differ depending on the medium's refractive index. (jascoinc.com)
  • Chiroptical methods, such as electronic and vibrational circular dichroism (CD, VCD) and vibrational optical activity (VOA) exploit the often small chiral asymmetries in light-matter interaction (see Figure 2), but are widely used techniques for the detection and structural determination of solvated chiral molecules (13). (chromatographyonline.com)
  • The efficiency of VCD technique as a reliable method to determine absolute configurations of various chiral molecules has expanded the scope of application vibrational circular dichroism spectrometer in drug discovery. (persistencemarketresearch.com)
  • We demonstrate theoretically that non-chiral perovskite layers can exhibit circular dichroism (CD) in the absence of a magnetic field and without chiral activation by chiral molecules. (arxiv.org)
  • Absolute configuration determination of chiral molecules in the solution state using vibrational circular dichroism. (semanticscholar.org)
  • Plasmonic circular dichroism (CD) of chiral molecules in the near field of plasmonic nanoparticles (NPs) may be used to enhance molecular CD signatures or to induce a CD signal at the plasmon resonance. (elsevier.com)
  • The server facilitates analyses using five popular algorithms and (currently) seven different reference databases by accepting data in a user-friendly manner in a wide range of formats, including those output by both commercial CD instruments and synchrotron radiation-based circular dichroism beamlines, as well as those produced by spectral processing software packages. (nih.gov)
  • We compare, for the first time, magnetite produced by these three different methods using X-ray magnetic circular dichroism (XMCD), a synchrotron radiation based technique able to determine the site occupancy of Fe cations in the ferrite spinels. (geoscienceworld.org)
  • We have used velocity map photoelectron imaging to study circular dichroism of the photoelectron angular distributions (PADs) of nitric oxide following two-color resonanceenhanced two-photon ionization via selected rotational levels of the A 2 Σ + , v' = 0 state. (osti.gov)
  • Appling, J. R. and White, M. G. and Dubs, R. L. and Dixit, S. N. and McKoy, V. (1987) Circular dichroism in photoelectron angular distributions from two‐color (1+1) REMPI of NO. Journal of Chemical Physics, 87 (12). (caltech.edu)
  • The published reports over the last 22 years have established VCD as a powerful technique with improved results over those previously obtained by visible/UV circular dichroism (CD) or optical rotatory dispersion (ORD) for proteins and nucleic acids. (wikipedia.org)
  • Iron-Chromophore Circular Dichroism of [Fe]-Hydrogenase The Conformational Change Required for H2 Activation. (docme.ru)
  • Circular dichroism (CD) is a fast and relatively easy spectroscopic technique to study protein conformational behaviour in solution. (springer.com)
  • The buffer or solvent the sample is made up in will also affect the circular dichroism spectrum. (jascoinc.com)
  • Dimers of bacteriochlorophyll a (Bchl a ) with optical absorption maximum at 853 nm and a nonconservative circular dichroism spectrum are formed in a solution of formamide/water that contains micelles of Triton X-100. (pnas.org)
  • Here we present the fabrication and characterization of quadruple-gyroid 4srs nanostructures exhibiting bio-inspired Bragg-mirror-like circular dichroism: a smooth and uniform band of circular dichroism reminiscent of the spectrum of a simple multilayer Bragg-mirror. (edu.au)
  • Vibrational Circular Dichroism Spectrometer market study with accurate historical and forecast projections, market size, market share, key industry trends, segment-wise analysis, comprehensive analysis on challenges, and opportunities for Vibrational Circular Dichroism Spectrometer market players, and impact of regulations. (persistencemarketresearch.com)
  • The growth of the vibrational circular dichroism spectrometer market is expected to remain influenced with the increasing use of vibrational circular dichroism as a platform to analyze peptide based properties, which is used for assessing various illnesses. (persistencemarketresearch.com)
  • This aspect can be considered as a key growth determinant of the vibrational circular dichroism spectrometer market. (persistencemarketresearch.com)
  • This aspect coupled with significant growth in the pharmaceutical sector is expected to auger well for the vibrational circular dichroism spectrometer market during the period of forecast. (persistencemarketresearch.com)
  • This factor is trending in the vibrational circular dichroism spectrometer, in turn complementing to the growth of the vibrational circular dichroism spectrometer market. (persistencemarketresearch.com)
  • The report on vibrational circular dichroism spectrometer market has segmented the market in detail to cover every facet of vibrational circular dichroism spectrometer. (persistencemarketresearch.com)
  • The vibrational circular dichroism spectrometer market is segmented on the basis of type and application. (persistencemarketresearch.com)
  • The report on vibrational circular dichroism spectrometer market includes analysis of key regions with respect to adoption and sales of vibrational circular dichroism spectrometer. (persistencemarketresearch.com)
  • The limit of detection of a circular dichroism (CD) spectrophotometer (or any spectrophotometer) is determined by its S/N characteristics: the better the S/N, the better its limit of detection. (labonline.com.au)
  • Analyzing protein circular dichroism spectra for accurate secondary structures. (springer.com)
  • The effects due to solvent on stabilizing the structures (conformers and zwitterionic species) of amino acids and peptides and the corresponding effects seen in the vibrational circular dichroism (VCD) and Raman optical activity spectra (ROA) have been recently documented by a combined theoretical and experimental work on L-alanine and N-acetyl L-alanine N'-methylamide. (wikipedia.org)
  • The study explores in vitro by circular dichroism and mass spectrometry the effects of pH, Cu+2 ions and sheetbreakers on the secondary structures and self-aggregation of b-amyloid peptides [A�43, A�42 and Ab40] of Alzheimer's disease. (ebscohost.com)
  • Circular dichroism (CD) is a powerful technique for studying the structures of proteins in solution, as well as structural changes that may occur when proteins bind to ligands. (currentprotocols.com)
  • The DICHROWEB web server enables on-line analyses of circular dichroism (CD) spectroscopic data, providing calculated secondary structure content and graphical analyses comparing calculated structures and experimental data. (nih.gov)
  • In this way, the in-plane chiral structures (metasurfaces) can exhibit comparable differential absorption of the left and right circular polarizations of light to that of the graphene nanodisks assemblies. (aps.org)
  • X-ray crystallography is still the only routine method for determining the three-dimensional structures of biological macromolecules at high resolution (Kühlbrandt, 1988) which can be utilized for the comparison of the structures determined by circular dichroism (CD) deconvolution. (springer.com)
  • Vibrational circular dichroism spectrometer, a recent technique, is widely used to analyze peptide structures. (persistencemarketresearch.com)
  • Protein secondary structures can also be determined experimentally from circular dichroism, and infrared spectroscopic data using empirical analysis methods. (biomedcentral.com)
  • Efficient differentiation between the left and right circular polarization of soft x-ray synchrotron radiation is achievable by doubling the number of prefocusing mirrors in a Dragon monochromator. (osapublishing.org)
  • However, the simple nature of Bragg mirror reflection prevents their application to the control of important polarization states such as circular polarization. (edu.au)
  • Furthermore, we demonstrate that the circular dichroism produced by 4srs nanostructures are robust to changes in incident angle and beam collimation, providing a new platform to create and engineer circular dichroism for functional circular polarization manipulation. (edu.au)
  • Circular polarization modulation was achieved by a combination of a wire grid polarizer and a photoelastic modulator (PEM) made of ZnSe. (nii.ac.jp)
  • X-ray magnetic circular dichroism (XMCD) is a powerful tool for the element-specific study of the magnetic structure of complex systems. (esrf.fr)
  • The effect of magnetic dichroism was foreseen for X-ray absorption spectra (XAS) almost 25 years ago [2] and the first experimental observations of linear and circular X-ray magnetic dichroism (XMCD) spectra were subsequently reported [3]. (esrf.fr)
  • We show that graphene plasmonic metasurfaces can create strong circular dichroism (CD) in mid-infrared due to the nanostructure's geometrical chirality and the plasmonic coupling between the elements in a chiral unit. (aps.org)
  • Chulhai, DV & Jensen, L 2015, ' Plasmonic circular dichroism of 310- and α-helix using a discrete interaction model/quantum mechanics method ', Journal of Physical Chemistry A , vol. 119, no. 21, pp. 5218-5223. (elsevier.com)
  • Circular dichroism in ion yield is achieved by resonance-enhanced multiphoton ionization. (ebscohost.com)
  • One- and two-photon circular dichroism spectra of R-(+)-3-methyl-cyclopentanone, a system that has been the subject of recent experimental studies of (2+1) resonance-enhanced multiphoton ionization circular dichroism, have been calculated with an origin-invariant density functional theory. (ebscohost.com)
  • Exploiting an effective wavevector for superchiral beams, natural circular dichroism (NCD) is derived from electric dipole - magnetic dipole (E1-M1) and electric dipole - electric quadrupole (E1-E2) absorption events, and MCD is derived from electric quadrupole-electric quadrupole (E2-E2) absorption. (arxiv.org)
  • Vibrational circular dichroism, which uses light from the infrared energy region, is used for structural studies of small organic molecules, and most recently proteins and DNA. (wikipedia.org)
  • The binding to human serum albumin (HSA) of the antitumoural drug paclitaxel and of several structural analogues has been characterized by bioaffinity chromatography and circular dichroism. (uniprot.org)
  • The experimental absorption and circular dichroism spectra of the in vitro Bchl dimer (termed Bchl-853) are similar to the spectra of the bacterial light-harvesting complex B850 and the primary electron donor P-860 and probably point to a common structural motif. (pnas.org)
  • Vibrational circular dichroism spectrometer provides a structural information in a three-dimensional format. (persistencemarketresearch.com)
  • We report here a structural study of the 99 residue Tat Eli variant using 2D liquid-state NMR, molecular modeling and circular dichroism. (semanticscholar.org)
  • On the other hand, strong CD, circular selective reflection and circular polarized luminescence were observed for optically active polyfluorene derivative films, showing that chiral discrimination arises from intermolecular interaction in the condensed phase. (nature.com)
  • Since JASCO circular dichroism spectrophotometers offer a continuous scan mode (and step-scan mode), the D.I.T. must be taken into account when setting the scanning speed. (jascoinc.com)
  • Circular dichroism yields valuable information about the configuration of organic molecules having a chromophore in the vicinity of their center of asymmetry. (docme.ru)
  • Whereas measurable dichroism occurs only in the region of absorption of the responsible chromophore, optical rotation extends well beyond this range, although with diminishing magnitude. (docme.ru)
  • In compounds having several chromophores near the asymmetric center, or a chromophore with several absorption bands, a detailed investigation and assignment of the separate effects is often possible only with the aid of circular dichroism. (docme.ru)
  • Determination of a low-level percent enantiomer of a compound with no ultraviolet chromophore using vibrational circular dichroism (VCD): enantiomeric purity by VCD of a compound with three chiral centers. (sparrho.com)
  • Circular dichroism and circular birefringence are manifestations of optical activity. (wikipedia.org)
  • Binding studies of taxanes to human serum albumin by bioaffinity chromatography and circular dichroism. (uniprot.org)
  • Although Vibrational Optical Activity and in particular Vibrational Circular Dichroism, has been known for a long time, the first VCD instrument was developed in 1973 and commercial instruments were available only since 1997. (wikipedia.org)
  • Two-photon circular dichroism spectra calculated within an origin-invariant density functional theory approximation in the absorption region where the lowest electronic excited states appear are presented for all 19 essential amino acids in the gas phase. (diva-portal.org)
  • Advances in the measurement, calculation, and application of vibrational circular dichroism (VCD) for the determination of absolute configuration are described. (semanticscholar.org)
  • For circular dichroism it is, however, particularly worthwhile to learn more about the principles and selection of the method of measurement, since considerable difficulties in instrumentation had to be surmouted. (docme.ru)
  • A vibrational circular dichroism (VCD) methodology for the measurement of enantiomeric excess in chiral compounds in the solid phase and for the complementary use of NMR and VCD techniques in solution: the camphor case. (sparrho.com)
  • As a consequence, circular dichroism is exhibited by biological molecules, because of the dextrorotary (e.g. some sugars) and levorotary (e.g. some amino acids ) molecules they contain. (bionity.com)
  • The results demonstrate that circular dichroism is observed even when cylindrical symmetry is maintained, and serve as a reminder that dichroism is a general feature of the multiphoton ionization of atoms and molecules. (osti.gov)
  • Circular Dichroism is a well-established technique for studying optical isomers (chirality) and secondary structure such as alpha / double helix in biological molecules. (technologynetworks.com)
  • We present the technical implementation of far-UV circular dichroism in a nanosecond T-jump experiment and show that this experiment allows us to follow quantitatively the change in the helical fraction of a poly(glutamic acid) peptide during its thermal denaturation with 12 ns time resolution. (ebscohost.com)
  • Circular dichroism showed complex results, in which AA and DTPA were strong modifiers of the percentages of the alpha-helical structure of HSA, while ribose affected the structure of HSA only at later time points. (mdpi.com)
  • Here, we present a novel kinetic assay that takes advantage of the loss of helical circular dichroic signals of insulin with IDE-dependent degradation. (portlandpress.com)
  • While helical and gyroid-based nanostructures exhibiting circular dichroism have been developed extensively to address this limitation, they are often restricted by the spectral inconsistency of their optical response. (edu.au)
  • The selective coupling of these helical exciton states to helical light is shown to give rise to circular dichroism. (arxiv.org)
  • To examine the validity of the model proposed, one of the approaches is to change the environment influencing the thermodynamic properties of a protein using denaturant agents, and observe the global and local protein conformation changes by, for instance, SAXS or circular dichroism (CD). (iucr.org)
  • The global circular dichroism spectrometers market is foreseen to expand at a CAGR of over 6.0% within the forecast period from 2017 to 2025 to reach roughly near about US$51.0 mn by the end of 2025. (sbwire.com)
  • On the basis of region, in 2016, North America was accounted for maximum share of the global circular dichroism (CD) spectrometers market. (sbwire.com)
  • Circular dichroism experiments supported this hypothesis. (uniprot.org)
  • Phosphorylation effect on the GSSS peptide conformation in water: Infrared, vibrational circular dichroism, and circular dichroism experiments and comparisons with molecular dynamics simulations. (ebscohost.com)
  • We have determined the magnetic ground state of the half-metal CrO 2 based on 1s2p Resonant Inelastic X-ray Scattering Magnetic Circular Dichroism (RIXS-MCD) experiments. (archives-ouvertes.fr)
  • Ab initio study of the one- and two-photon circular dichroism of R-(+)-3-methyl-cyclopentanone. (ebscohost.com)
  • Circular dichroism is used to study the conformation of proteins in solution. (emf-portal.org)
  • Local X-ray magnetic circular dichroism study of Fe/Cu(111) using a tunneling smart tip. (escholarship.org)
  • A synchrotron X-ray scanning tunneling microscope has been employed for the local study of the X-ray magnetic circular dichroism at the Fe L2,3-edges of a thin iron film grown on Cu(111). (escholarship.org)
  • The 'Global and Southeast Asia Vibrational Circular Dichroism Spectrometer Industry, 2013-2023 Market Research Report' is a professional and in-depth study on the current state of the global Vibrational Circular Dichroism Spectrometer industry with a focus on the Southeast Asia market. (marketstudyreport.com)
  • Circular dichroism spectra revealed that the network made up of oligomers C+D showed more helicity (15 m°) than oligomers A+B (13 m°) at 272 nm ( Fig. 3a ), as expected. (scielo.org.za)
  • The magnetic circular dichroism and absorption spectra of Ta atoms isolated in an Ar matrix have been observed in the range 220 - 540 nm. (osu.edu)
  • Vibrational circular dichroism spectrometer is a device that uses VCD (vibrational circular dichroism) platform, which is being used across various clinical research programs to measure enantiomeric excess in solid phase compounds reflecting chiral properties. (persistencemarketresearch.com)
  • Vibrational circular dichroism spectrometer demand is also influenced by growing use of vibrational circular dichroism spectrometer in quantitative and qualitative identification of compounds. (persistencemarketresearch.com)
  • Analysis of the major chiral compounds of Artemisia herba-alba essential oils (EOs) using reconstructed vibrational circular dichroism (VCD) spectra: En route to a VCD chiral signature of EOs. (sparrho.com)
  • Single peptide bonds exhibit poly(pro)II ("random coil") circular dichroism spectra. (springer.com)
  • Phillips-Jones MK, Patching SG, Edara S, Nakayama J, Hussain R, Siligardi G (2013) Interactions of the intact FsrC membrane histidine kinase with the tricyclic peptide inhibitor siamycin I revealed through synchrotron radiation circular dichroism. (springer.com)