Circovirus: A genus of the family CIRCOVIRIDAE that infects SWINE; PSITTACINES; and non-psittacine BIRDS. Species include Beak and feather disease virus causing a fatal disease in psittacine birds, and Porcine circovirus causing postweaning multisystemic wasting syndrome in pigs (PORCINE POSTWEANING MULTISYSTEMIC WASTING SYNDROME).Circoviridae Infections: Virus diseases caused by the CIRCOVIRIDAE.Wasting Syndrome: A condition of involuntary weight loss of greater then 10% of baseline body weight. It is characterized by atrophy of muscles and depletion of lean body mass. Wasting is a sign of MALNUTRITION as a result of inadequate dietary intake, malabsorption, or hypermetabolism.Porcine Postweaning Multisystemic Wasting Syndrome: A worldwide emerging disease of weaned piglets first recognized in swine herds in western Canada in 1997. This syndrome is characterized by progressive weight loss, rapid (tachypnea) and difficult (dyspnea) breathing, and yellowing of skin. PMWS is caused by PORCINE CIRCOVIRUS infection, specifically type 2 or PCV-2.Swine Diseases: Diseases of domestic swine and of the wild boar of the genus Sus.Circoviridae: A family of very small viruses containing circular, single-stranded DNA and possessing no envelope. The modes of transmission are not known.Swine: Any of various animals that constitute the family Suidae and comprise stout-bodied, short-legged omnivorous mammals with thick skin, usually covered with coarse bristles, a rather long mobile snout, and small tail. Included are the genera Babyrousa, Phacochoerus (wart hogs), and Sus, the latter containing the domestic pig (see SUS SCROFA).Parvovirus, Porcine: A species of PARVOVIRUS causing reproductive failure in pigs.Porcine respiratory and reproductive syndrome virus: A species of ARTERIVIRUS causing reproductive and respiratory disease in pigs. The European strain is called Lelystad virus. Airborne transmission is common.DNA, Viral: Deoxyribonucleic acid that makes up the genetic material of viruses.Genome, Viral: The complete genetic complement contained in a DNA or RNA molecule in a virus.Porcine Reproductive and Respiratory Syndrome: A syndrome characterized by outbreaks of late term abortions, high numbers of stillbirths and mummified or weak newborn piglets, and respiratory disease in young unweaned and weaned pigs. It is caused by PORCINE RESPIRATORY AND REPRODUCTIVE SYNDROME VIRUS. (Radostits et al., Veterinary Medicine, 8th ed, p1048)Antibodies, Viral: Immunoglobulins produced in response to VIRAL ANTIGENS.Colostrum: The thin, yellow, serous fluid secreted by the mammary glands during pregnancy and immediately postpartum before lactation begins. It consists of immunologically active substances, white blood cells, water, protein, fat, and carbohydrates.Capsid Proteins: Proteins that form the CAPSID of VIRUSES.Viral Vaccines: Suspensions of attenuated or killed viruses administered for the prevention or treatment of infectious viral disease.Bird Diseases: Diseases of birds not considered poultry, therefore usually found in zoos, parks, and the wild. The concept is differentiated from POULTRY DISEASES which is for birds raised as a source of meat or eggs for human consumption, and usually found in barnyards, hatcheries, etc.Weaning: Permanent deprivation of breast milk and commencement of nourishment with other food. (From Stedman, 25th ed)Polymerase Chain Reaction: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.Psittaciformes: An order of BIRDS comprised of several families and more than 300 species. It includes COCKATOOS; PARROTS; PARAKEETS; macaws; and BUDGERIGARS.Torque teno virus: A species of non-enveloped DNA virus in the genus ANELLOVIRUS, associated with BLOOD TRANSFUSIONS; and HEPATITIS. However, no etiological role has been found for TTV in hepatitis.Viremia: The presence of viruses in the blood.Open Reading Frames: A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).Molecular Sequence Data: Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.DucksEncyclopedias as Topic: Works containing information articles on subjects in every field of knowledge, usually arranged in alphabetical order, or a similar work limited to a special field or subject. (From The ALA Glossary of Library and Information Science, 1983)Terminology as Topic: The terms, expressions, designations, or symbols used in a particular science, discipline, or specialized subject area.Intellectual Property: Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)VirginiaPatents as Topic: Exclusive legal rights or privileges applied to inventions, plants, etc.Chicken anemia virus: The type species of GYROVIRUS, a small, non-enveloped DNA virus originally isolated from contaminated vaccines in Japan. It causes chicken infectious anemia and may possibly play a key role in hemorrhagic anemia syndrome, anemia dermatitis, and blue wing disease.Beak: In some animals, the jaws together with their horny covering. The beak usually refers to the bill of birds in which the whole varies greatly in form according of the food and habits of the bird. While the beak refers most commonly to birds, the anatomical counterpart is found also in the turtle, squid, and octopus. (From Webster, 3d ed & Storer, et al., General Zoology, 6th ed, p491, 755)Feathers: Flat keratinous structures found on the skin surface of birds. Feathers are made partly of a hollow shaft fringed with barbs. They constitute the plumage.Influenza Vaccines: Vaccines used to prevent infection by viruses in the family ORTHOMYXOVIRIDAE. It includes both killed and attenuated vaccines. The composition of the vaccines is changed each year in response to antigenic shifts and changes in prevalence of influenza virus strains. The vaccine is usually bivalent or trivalent, containing one or two INFLUENZAVIRUS A strains and one INFLUENZAVIRUS B strain.Vaccines: Suspensions of killed or attenuated microorganisms (bacteria, viruses, fungi, protozoa), antigenic proteins, synthetic constructs, or other bio-molecular derivatives, administered for the prevention, amelioration, or treatment of infectious and other diseases.Influenza, Human: An acute viral infection in humans involving the respiratory tract. It is marked by inflammation of the NASAL MUCOSA; the PHARYNX; and conjunctiva, and by headache and severe, often generalized, myalgia.Income: Revenues or receipts accruing from business enterprise, labor, or invested capital.Influenza A Virus, H1N1 Subtype: A subtype of INFLUENZA A VIRUS with the surface proteins hemagglutinin 1 and neuraminidase 1. The H1N1 subtype was responsible for the Spanish flu pandemic of 1918.Vaccination: Administration of vaccines to stimulate the host's immune response. This includes any preparation intended for active immunological prophylaxis.Vaccines, Inactivated: Vaccines in which the infectious microbial nucleic acid components have been destroyed by chemical or physical treatment (e.g., formalin, beta-propiolactone, gamma radiation) without affecting the antigenicity or immunogenicity of the viral coat or bacterial outer membrane proteins.OhioProduct Labeling: Use of written, printed, or graphic materials upon or accompanying a product or its container or wrapper. It includes purpose, effect, description, directions, hazards, warnings, and other relevant information.KansasAntigens, Heterophile: Antigens stimulating the formation of, or combining with heterophile antibodies. They are cross-reacting antigens found in phylogenetically unrelated species.Recombinases: A broad category of enzymes that are involved in the process of GENETIC RECOMBINATION.Nucleic Acid Amplification Techniques: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.ArchivesBiological Science Disciplines: All of the divisions of the natural sciences dealing with the various aspects of the phenomena of life and vital processes. The concept includes anatomy and physiology, biochemistry and biophysics, and the biology of animals, plants, and microorganisms. It should be differentiated from BIOLOGY, one of its subdivisions, concerned specifically with the origin and life processes of living organisms.Periodicals as Topic: A publication issued at stated, more or less regular, intervals.PubMed: A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.Lysosomes: A class of morphologically heterogeneous cytoplasmic particles in animal and plant tissues characterized by their content of hydrolytic enzymes and the structure-linked latency of these enzymes. The intracellular functions of lysosomes depend on their lytic potential. The single unit membrane of the lysosome acts as a barrier between the enzymes enclosed in the lysosome and the external substrate. The activity of the enzymes contained in lysosomes is limited or nil unless the vesicle in which they are enclosed is ruptured. Such rupture is supposed to be under metabolic (hormonal) control. (From Rieger et al., Glossary of Genetics: Classical and Molecular, 5th ed)

Identification of a novel GC-rich 113-nucleotide region to complete the circular, single-stranded DNA genome of TT virus, the first human circovirus. (1/377)

The sequence data (H. Okamoto et al., Hepatol. Res. 10:1-16, 1998) of a newly discovered single-stranded DNA virus, TT virus (TTV), showed that it did not have the terminal structure typical of a parvovirus. Elucidation of the complete genome structure was necessary to understand the nature of TTV. We obtained a 1.0-kb amplified product from serum samples of four TTV carriers by an inverted, nested long PCR targeted for nucleotides (nt) 3025 to 3739 and 1 to 216 of TTV. The sequence of a clone obtained from serum sample TA278 was compared with those registered in GenBank. The complete circular TTV genome contained a novel sequence of 113 nt (nt 3740 to 3852 [=0]) in between the known 3'- and 5'-end arms, forming a 117-nt GC-rich stretch (GC content, 90.6% at nt 3736 to 3852). We found a 36-nt stretch (nt 3816 to 3851) with an 80.6% similarity to chicken anemia virus (CAV) (nt 2237 to 2272 of M55918), a vertebrate circovirus. A putative SP-1 site was located at nt 3834 to 3839, followed by a TATA box at nt 85 to 90, the first initiation codon of a putative VP2 at nt 107 to 109, the termination codon of a putative VP1 at nt 2899 to 2901, and a poly(A) signal at nt 3073 to 3078. The arrangement was similar to that of CAV. Furthermore, several AP-2 and ATF/CREB binding sites and an NF-kappaB site were arranged around the GC-rich region in both TTV and CAV. The data suggested that TTV is circular and similar to CAV in its genomic organization, implying that TTV is the first human circovirus.  (+info)

Evidence that a plant virus switched hosts to infect a vertebrate and then recombined with a vertebrate-infecting virus. (2/377)

There are several similarities between the small, circular, single-stranded-DNA genomes of circoviruses that infect vertebrates and the nanoviruses that infect plants. We analyzed circovirus and nanovirus replication initiator protein (Rep) sequences and confirmed that an N-terminal region in circovirus Reps is similar to an equivalent region in nanovirus Reps. However, we found that the remaining C-terminal region is related to an RNA-binding protein (protein 2C), encoded by picorna-like viruses, and we concluded that the sequence encoding this region of Rep was acquired from one of these single-stranded RNA viruses, probably a calicivirus, by recombination. This is clear evidence that a DNA virus has incorporated a gene from an RNA virus, and the fact that none of these viruses code for a reverse transcriptase suggests that another agent with this capacity was involved. Circoviruses were thought to be a sister-group of nanoviruses, but our phylogenetic analyses, which take account of the recombination, indicate that circoviruses evolved from a nanovirus. A nanovirus DNA was transferred from a plant to a vertebrate. This transferred DNA included the viral origin of replication; the sequence conservation clearly indicates that it maintained the ability to replicate. In view of these properties, we conclude that the transferred DNA was a kind of virus and the transfer was a host-switch. We speculate that this host-switch occurred when a vertebrate was exposed to sap from an infected plant. All characterized caliciviruses infect vertebrates, suggesting that the host-switch happened first and that the recombination took place in a vertebrate.  (+info)

Ultrastructure of porcine circovirus in persistently infected PK-15 cells. (3/377)

The ultrastructure of porcine circovirus was examined in persistently infected porcine kidney (PK)-15 cells. Virus-infected PK-15 cells had large numbers of intracytoplasmic inclusions, and a few cells had intranuclear inclusions. Intracytoplasmic inclusions were dispersed throughout the cytoplasm but were most numerous in the perinuclear cytoplasm. Inclusion were of various sizes, round to oval, and electron dense and were of two general types. Inclusions of the first type were small (0.1-0.5 microm diameter), not surrounded by trilaminar membranes, and granular with indistinct margins that blended with surrounding cytoplasm. Some contained 12+/-2-nm-diameter icosahedral virions in loose aggregates or rarely forming paracrystalline arrays. Small inclusions could be sites of viral assembly or maturation. Intracytoplasmic inclusions of the second type were larger (0.5-5.0 microm diameter) and more numerous and had abrupt margins surrounded by trilaminar membranes. They were more electron dense than small inclusions and were heterogeneous, containing various proportions of aggregated virions, electron-dense crystalline lamellae of 5 nm periodicity, and/or whorls of myelinoid membranes. Virions usually formed paracrystalline arrays and occasionally were loosely aggregated. Larger inclusions were typical of autophagolysosomes. Intranuclear inclusions were not membrane bound and were often associated with reticulated nucleoli or aggregates of heterochromatin. Some inclusions were irregularly shaped aggregates of indistinct, circular 10-12-nm-diameter viruslike particles. Others were 0.1-1.0 microm in diameter, round or ring shaped, dense, and finely granular, with sharply demarcated margins.  (+info)

Electron microscopical observations of psittacine beak and feather disease in an Umbrella cockatoo (Cacatua alba). (4/377)

Psittacine beak and feather disease (PBFD) was diagnosed in an umbrella cockatoo (Cacatua alba) with severe feather dystrophy and loss. Electron microscopically, the intranuclear and intracytoplasmic inclusion bodies observed by light microscopy were composed of viral particles forming paracrystalline arrays, whorls, semicircles or concentric circles. Recovered viral particles from the skin and feather follicle tracts were icosahedral and 15 to 20 nm in diameter.  (+info)

Detection of porcine circovirus from lesions of a pig with wasting disease in Japan. (5/377)

A wasting disease characterized by progressive weight loss and dyspnea has been observed in weaning pigs on a farm in Yamagata Prefecture in 1998. Histopathologic findings in an affected pig were bronchointerstitial pneumonia and intracytoplasmic clusters of basophilic inclusions in macrophages of lymph nodes, which were similar to those in pigs with postweaning multisystemic wasting syndrome (PMWS) recently reported in North America and Europe. Porcine circovirus (PCV)-like particles were observed in bronchial lymph node of the pig by electron microscopy, and PCV antigens were detected in the lesions by immunohistochemical staining. PCV DNA was also detected in the lung and tonsil by PCR, and restriction fragment length polymorphism analysis of the PCR products with HinfI showed the same type of the PCV associated with PMWS (pmws PCV). Homology of nucleotide sequences between the PCR product and corresponding regions of published pmws PCV genomes was very high. These results indicated that virus detected in this study was pmws PCV. To our knowledge, this is the first report on the presence of pmws PCV in Japan.  (+info)

Multiplex PCR for detection and typing of porcine circoviruses. (6/377)

Sets of oligonucleotide primers were designed according to the sequences of the open reading frames (ORFs) ORF1 and ORF2 of the prototype nonpathogenic PK-15 strain of porcine circovirus (PCV) type 1 (PCV-1). By the PCR performed with the various primer sets, genomic DNA or RNA from other bacterial or viral pathogens of the respiratory tracts of pigs could not be amplified. A positive amplification reaction could be visualized with DNA extracted from a viral suspension containing as few as 10 viral particles per ml. No DNA fragment could be amplified from lysates of continuous porcine cell lines (PT, ST, and PFT cells) known to be negative for PCV. When tested with clinical samples from pigs, the results of the single PCR method showed nearly 93% (13 of 14 samples) correlation with histopathological and immunohistochemical findings. Interestingly, subclinical PCV infections could be detected by single PCR with clinical samples that have been submitted from animals with irrelevant cases of respiratory and/or enteric problems. On the basis of the nucleotide sequences of PCV strains (PCV-2) recently associated with outbreaks of postweaning multisystemic wasting syndrome (PWMS) in Quebec, Canada, pig farms, other primers were designed from the PCV-1 genome, and these primers failed to amplify genomic fragments specific to the ORF1 or ORF2 genes of clinical isolates associated with PWMS but amplified DNA from the PCV-1 strain. Two rapid multiplex PCR (mPCR) methods have been developed to distinguish between both genotypes of PCV. By those two mPCR methods, (i) species-specific primer pairs were used to amplify a DNA fragment of 488 bp specific for the ORF2 genes of both genotypes, whereas a 375-bp fragment was amplified from the ORF1 gene of the PCV-1 strain only, or (ii) species-specific primer pairs were used to amplify a DNA fragment of 646 bp specific for the ORF1 genes of both genotypes, whereas a 425-bp fragment was amplified from the ORF2 gene of the PCV-1 strain only. By both mPCR methods, a PCV-2 infection was demonstrated in tissues of 94.2% (33 of 35) of the sick pigs tested, in agreement with previous findings showing the close association of this new genotype of PCV with outbreaks of PMWS in Europe and North America. On the other hand, a PCV-1 infection was confirmed in only 5.7% (2 of 35) of the pigs, and confirmation of a mixed infection with PCV-2 was obtained by a single PCR with PCV-2-specific primers.  (+info)

PCR detection and characterization of type-2 porcine circovirus. (7/377)

A polymerase chain reaction (PCR) assay was developed for detecting porcine circovirus (PCV). The assay readily detected type-2 PCV (PCV-2) and type-1 PCV (PCV-1). The PCR primers were designed based on DNA sequences conserved in all reported PCV genomes. Type 1 PCV and type 2 PCV both produced 438 bp amplification products, which were easily identified and differentiated from one another by restriction fragment length polymorphism (RFLP) analysis. Porcine circovirus was detected in 55% (931/1693) of randomly tested pigs with various clinical signs and lesions, most of which were difficult to differentiate from those associated with porcine reproductive and respiratory syndrome (PRRS). The PCR products from all positive clinical samples were identified by RFLP to be only PCV-2; DNA tested by PCR was extracted directly from one or more of lung, mesenteric or mediastinal lymph nodes, and tonsil. Type 2 PCV was also detected in 6% (2/34) of DNA extracted directly from semen of randomly chosen healthy boars. Positive PCR reactions from 554 diseased pigs were characterized by RFLP and categorized into 5 different profiles (A-E), of which 82.8% were PCV-2A (456/554), 3.0% were PCV-2B (17/554), 9.9% were PCV-2C (55/554), 1.1% were PCV-2D (6/554), and 3.2% were PCV-2E (18/554). The complete genomic nucleotide sequences of PCV-2A, B, C, D, and E were determined and found to have at least 95% homology compared with one another and with all other PCV-2 found in the GenBank database. All PCV-2 had less than 76% homology with PCV-1. This PCR assay will hopefully be useful to veterinary diagnostic laboratories for routine testing and surveillance of infection with PCV-2. The RFLP profiling system might be useful for preliminary characterization and identification of PCV isolates and might also benefit studies on the molecular epidemiology of PCV.  (+info)

Porcine circoviruses: a review. (8/377)

Porcine circoviruses (PCV) are small nonenveloped DNA viruses containing a unique single-stranded circular genome. Previously, no recognized link was found between PCV infection of pigs and disease, and PCV was considered a nonpathogenic agent. Over the last 5 years, a "novel" PCV, designated PCV2, has been associated with various disease syndromes in pigs, primarily postweaning multisystemic wasting syndrome (PMWS). Pigs with PMWS have a variety of clinical signs, including debility, dyspnea, palpable lymphadenopathy, diarrhea, and pallor or icterus. Lesions associated with the presence of PCV2 in a variety of cell types include lymphohistiocytic to granulomatous interstitial pneumonia, hepatitis, nephritis, myocarditis, enteritis, and pancreatitis. The lesions of PMWS have been reproduced experimentally after inoculation of piglets with PCV2 cell culture isolates, although the full expression of the disease syndrome may require the presence of other agents such as porcine parvovirus or porcine reproductive and respiratory syndrome (PRRS) virus. Recent reports have linked PCV2 to other disorders in pigs, ranging from abortion and reproductive failure to "atypical" PRRS. Available data indicate high seroprevalence of antibodies to PCV2 worldwide. The diagnosis of PCV2-associated disease is based on the direct demonstration of PCV2 antigens or nucleic acid in affected tissues. PCV2 is now regarded as an important emerging pathogen. Although vertical transmission has been documented, the epidemiology of PCV2 infections is poorly understood, as is the role of the immune response in controlling or augmenting disease.  (+info)

1. Meehan BM, McNeilly F, Todd D, Kennedy S, Jewhurst VA, Ellis JA, Hassard LE, Clark EG, Haines DM, Allan GM. Characterization of novel circovirus DNAs associated with wasting syndromes in pigs. J Gen Virol. 1998;79:2171-2179.. 2. Chae C. A review of porcine circovirus 2-associated syndromes and diseases. Vet J. 2005;169:326-336.. 3. Chae C. Postweaning multisystemic wasting syndrome: a review of aetiology, diagnosis and pathology. Vet J. 2004;168:41-49.. 4. Chae C. Commercial porcine circovirus type 2 vaccines: Efficacy and clinical application. Vet J. 2012;194:151-157.. 5. Chae C. Porcine circovirus type 2 and its associated disease in Korea. Virus Res. 2012;164:107-113.. 6. Fenaux M, Opriessnig T, Halbur PG, Elvinger F, Meng XJ. A chimeric porcine circovirus (PCV) with the immunogenic capsid gene of the pathogenic PCV type 2 (PCV2) cloned into the genomic backbone of the nonpathogenic PCV1 induces protective immunity against PCV2 infection in pigs. J Virol. 2004;78:6297-6303.. 7. Chow SC, ...
Porcine circovirus-associated diseases (PCVAD), caused by porcine circovirus type 2 (PCV2), threaten the pig industry worldwide. Five genotypes of PCV2 were recently identified: PCV2a, PCV2b, PCV2c, PCV2d and PCV2e. In addition, a novel porcine circovirus from a case of a sow with dermatitis, nephropathy syndrome and reproductive failure has been identified based on metagenomic analysis and classified as porcine circovirus type 3 (PCV3). Therefore, the current study was conducted to determine the prevalence and genetic characteristics of PCV2 and PCV3 in clinical samples. A total of 471 samples (161 tissue samples of lungs and lymph nodes from 34 farms and 310 serum samples from 47 farms) were tested for PCV2. Among them, 171 samples from 59 farms that had been positive for PCV2 were genotyped. Another 690 samples (296 tissue samples of lungs and lymph nodes from 91 farms, 108 samples of aborted foetuses from 26 farms, and 286 serum samples from 47 farms) were tested for PCV3. Based on PCV2 genotyping
Porcine circoviral disease (PCVD) and Porcine circovirus associated disease (PCVAD), is a disease seen in domestic pigs. This disease causes illness in piglets, with clinical signs including progressive loss of body condition, visibly enlarged lymph nodes, difficulty in breathing, and sometimes diarrhea, pale skin, and jaundice. PCVD is very damaging to the pig-producing industry and has been reported worldwide. PCVD is caused by porcine circovirus type 2 (PCV-2). The North American industry endorses "PCVAD" and European use "PCVD" to describe this disease. Postweaning multisystemic wasting syndrome ("PMWS") is the classic PCVD entity, caused by PCV-2. PCV-2 has a near universal distribution - present in most pig herds. In contrast, PMWS is more sporadic in its distribution. Experimental induction of PMWS has not been achieved by PCV-2 infection alone, using infectious DNA clones of the virus or a pure form of PCV-2 derived from infectious DNA clones. Therefore, it is assumed that PMWS is a ...
Porcine circovirus type 2 (PCV2) causes postweaning multisystemic wasting syndrome (PMWS) in growing pigs but generally requires the presence of other infectious or non-infectious factors to produce the full clinical expression of disease. These factors remain to be identified, but management routines, concurrent infections and maternal immunity are thought to influence the risk for PMWS outbreak in swine herds. Pigs presenting with PMWS develop severe immunosuppression and the work of this thesis focuses on the interaction between PCV2 and the immune system of the pig in vivo and in vitro. Development of PMWS was studied in models for experimental infection in young pigs using coinfections with porcine parvovirus (PPV) or Escherichia coli. Three-day-old colostrum-deprived pigs infected with PCV2 and PPV developed severe PMWS, and a possible role for interleukin 10 as well as impaired production of antibodies to PCV2 in the development of disease was observed. Four-week-old colostrum fed pigs ...
Porcine circovirus type 2 (PCV2) belongs to the family Circoviridae, and is the causative agent of post-weaning multisystemic wasting syndrome (PMWS) in pigs. In this study, phylogenetic analyses of three complete PCV2 genomic sequences from Hong Kong suggest that natural recombination happened among different lineages of PCV2. A preliminary investigation of the parental strains of these potential recombinants was carried out using bootscanning. Statistical significance of this recombination event was tested and positions of the potential recombination breakpoints were estimated in a maximum-likelihood framework. The recombinant breakpoints were estimated to be located within the origin of replication (ori) and replicase (rep) gene of PCV2. Interestingly, several GenBank sequences of PCV2 in mainland China were found to have a recombination pattern similar to that of the potential PCV2 recombinants from Hong Kong, implying that this recombinant genotype might already be widespread within ...
Porcine circovirus type 2 Capsid antibody for IHC, IHC-P, WB. Anti-Porcine circovirus type 2 Capsid pAb (GTX128120) is tested in Porcine circovirus type 2 samples. 100% Ab-Assurance.
Baltimore Classification== ===Higher order taxa=== Viruses; ssDNA viruses; Circoviridae; Circovirus ===Species=== Beak and feather disease virus, Canary circovirus, Goose circovirus ==Description and Significance== Circoviruses are very small non-enveloped icosahedral viruses with a single stranded circular DNA genome. They have been described in pigs (porcine circovirus, PCV), chickens (chicken anemia virua, ChAV), psittacines (psittacine beak and feather disease virus, PBFD) and pigeons. The virus targets the lymphoid tissue and causes immunosuppression in the host. Circoviruses are the smallest pathogenic DNA viruses that have been identified and characterized in animals. ==Genome Structure== The genome of the circovirus is monomeric. It is unsegmented and contains a single molecule of circular, ambisense, sinle-stranded DNA that forms a covalently closed circle. The complete genome is 1800-2000 nucleotides long and the genome has a guanine+cytosine content of 48.4-53.1 %. (source: ...
TY - JOUR. T1 - Development of a polyclonal-antibody-based immunohistochemical method for the detection of type 2 porcine circovirus in formalin-fixed, paraffin-embedded tissue. AU - Sorden, Steven D.. AU - Harms, Perry A.. AU - Nawagitgul, Porntippa. AU - Cavanaugh, David. AU - Paul, Prem S.. PY - 1999/11. Y1 - 1999/11. N2 - An immunohistochemical method for the detection of type 2 porcine circovirus (PCV2) in paraffin-embedded tissue was developed. Rabbits were inoculated with purified PCV2 to obtain a polyclonal antiserum. Antiserum was applied to sections of porcine tissue that contained lesions consistent with postweaning multisystemic wasting syndrome and in which PCV2 genome had been demonstrated by in situ hybridization. In all cases (18/18), the density and distribution of positive cells detected by in situ hybridization or immunohistochemistry were identical. The immunohistochemical method is more rapid and less expensive than in situ hybridization and is thus more suitable for routine ...
IDENTIFICATION AND USE: Porcine circoviruses are small, icosahedral viruses that were discovered in 1974 as contaminants of a porcine kidney cell line. They were later called circoviruses when their genome was found to be a circular, single-stranded DNA molecule. Upon entry into cells, the viral ssDNA genome enters the nucleus where it is made double-stranded by host enzymes. It is then transcribed by host RNA polymerase II to form mRNAs that are translated into viral proteins. There is some evidence that circoviruses might have evolved from a plant virus that switched hosts and then recombined with a picorna-like virus.. Porcine circoviruses are classified in the Circoviridae family, which contains two genera, Circovirus and Gyrovirus. There are two porcine circoviruses, PCV-1 and PCV-2; only the latter causes disease in pigs. Infection probably occurs via oral and respiratory routes, and leads to various diseases including postweaning multisystemic wasting syndrome, and porcine dermatitis and ...
Porcine circovirus type 2 (PCV2) associated reproductive disease was diagnosed in a herd containing only gilts. A single case of abortion occurred and no other disorder was evident in the herd. PCV2 antigen and/or DNA were detected in two aborted fetuses. One of the fetuses, revealing both PCV2 DNA and antigen, presented multinucleated giant cells, severe vascular lesions (intramural oedema, fibrinoid necrosis, mild lympho-histiocytic vasculitis, fibrin thrombi) and mild non-suppurative inflammation in the lungs. Other abortifacient infections were not found. This is the only report of PCV2-induced abortion in Hungary since 1999, when PCV2-associated disease was first discovered in the country. ...
Porcine circovirus type 2 (PCV2) vaccination leads to reduced mortality, increased growth rate, inconsistent effects on feed efficiency and no impacts on carcass traits but the effects on nursery performance are product-dependent, according to Dr Steve Dritz of Kansas State University.
Coreplication of the major genotype group members of porcine circovirus type 2 as a prerequisite to coevolution may explain the variable disease ...
Open reading frame 2 (ORF2) of porcine circovirus type 2 (PCV2) codes for the 233 amino acid capsid protein (CP). Baculovirus-based vaccines that express only ORF2 are protective against clinical disease following experimental challenge or natural infection. The goal of this study was to identify regions in CP preferentially recognized by sera from experimentally infected and vaccinated pigs, and compare these responses to pigs diagnosed with porcine circovirus-associated disease (PCVAD). The approach was to react porcine sera with different CP polypeptide fragments that each contained one or more immunoreactive regions. Expression of polypeptides was performed using E.coli. Initial results showed that sera from vaccinated pigs preferentially recognized only the largest CP(43-233) polypeptide fragment and showed low levels of binding to other CP polypeptide fragments. The results of sera from pigs diagnosed with PMWS showed only minimal reactivity with CP polypeptide fragments, including the ...
Porcine circovirus type 2 (PCV2) is the primary causative agent of postweaning multisystemic wasting syndrome (PMWS), whereas the ubiquitous porcine circovirus type 1 (PCV1) is nonpathogenic for pigs. Since its initial detection in a Canadian commercial swine herd in 1991, PMWS has been detected in all swine producing regions of the world and is now a serious economic problem to the swine industry. The objectives of this dissertation were to biologically, genetically and experimentally characterize both PCV1 and PCV2, to identify the genetic determinant(s) for virulence and replication, and to develop an effective genetically-engineered vaccine against PCV2 infection and PMWS. The genetic heterogeneity of PCV2 and PCV1 isolates from different geographic origins were determined. We found that, although PCV1 and PCV2 genomes were very conserved, some minor genomic variation exists among PCV1 isolates and PCV2 isolates. The nonpathogenic PCV1 and pathogenic PCV2 share only about 76% nucleotide ...
The Duck circovirus (DuCV) is a type of virus found in ducks. Strains of the virus have predominantly been found in China, though strains have also been isolated from ducks in Germany and the United States. Duck circovirus is a small nonenveloped virus with a monomeric single-stranded circular DNA genome. DuCV has been clustered in the Circoviridae family genus Circovirus, according to the eighth report of the International Committee on Taxonomy of Viruses. Scientists have studied the Duck circovirus by using polymerase chain reaction (PCR)-based methods and dot blot hybridisation (DBH) tests. Infection with DuCV appears to cause growth disorders in ducks as well as eventual immunosuppression due to depletion of lymphatic cells. It was found that ducks between the ages of 40∼60 days were more susceptible to Duck circovirus. There was no evidence showing that the DuCV virus was capable of vertical transmission. The symptoms are immunosuppression, stunting in growth, and also feather ...
Porcine circovirus 2 (PCV2) is the primary cause of post-weaning multisystemic wasting syndrome, in which it stimulates a strong IL-8 response that is associated with chronic inflammation as well as lesions in the lymphoid organs. However, the mechanism underlying PCV2-induced IL-8 production is still unclear. In the present study, we demonstrated that increased IL-8 expression during PCV2 infection depends on Toll-like receptor (TLR2), but not TLR4 or TLR9 signalling pathways in porcine alveolar macrophages. Moreover, we found that impairment of the MyD88/NF-κB signalling pathway by MyD88 knockdown or NF-κB inhibitors markedly decreased PCV2-induced IL-8 secretion. These results suggest that PCV2 induces IL-8 secretion via the TLR2/MyD88/NF-κB signalling pathway. Therefore, it is important to elucidate the molecular mechanisms of the PCV2-induced inflammatory response.
1111767DNAPorcine circovirus 1accagcgcac ttcggcagcg gcagcacctc ggcagcacct cagcagcaac atgcccagca 60agaagagtgg aagaagcgga ccccaaccac ataaaaggtg ggtgttcacg ctgaataatc 120cttccgaaga cgagcgcaag aaaatacggg agctcccaat ctccctattt gattatttta 180ttgttggcga ggaaggtaat gaggagggcc gaacacccca cctacagggg ttcgctaatt 240ttgtgaagaa gcaaactttt aataaagtga agtggtattt tggtgcccgc tgccacatcg 300agaaagcgaa aggaacagat cagcagaata aagaatattg cagtaaagaa ggcaacttac 360tgatagaatg tggagctcct agatctcaag gacaacggag tgacctctct actgctgtga 420gtaccttgtt ggagagcggg agtctggtga ccgttgcaga gcagcaccct gtaacgtttg 480tcagaaattt ccgcgggctg gctgaacttt tgaaagtgag cgggaaaatg cagaagcgtg 540attggaagac gaatgtacac gtcattgtgg ggccacctgg gtgtggcaaa agcaaatggg 600ctgctaattt tgcagacccg gaaaccacat actggaaacc acctagaaac aagtggtggg 660atggttacca tggtgaagaa gtggttgtta ttgatgactt ttatggctgg ctgccgtggg 720atgatctact gagactctgt gatcgatatc ctttgactgt tgagactaaa ggtggaactg 780tacctttttt ggcccgcagt attctgatta ccagcaatca gaccccgttg gaatggtact 840cctcaactgc ...
Two types of porcine circovirus (PCV) exist, referred to as PCV1 and PCV2. PCV2 has been associated with disease syndromes in pigs, including that designated postweaning multisystemic wasting syndrome (PMWS), which has been identified in all regions of the world bar Australia (Hamel et al., 1998; Allan et al., 1999a; Onuki et al., 1999; Martelli et al., 2000; Kyriakis et al., 2000; Wellenberg et al., 2000; Done et al., 2001; Trujano et al., 2001; Saradell et al., 2004; Castro et al., 2004; Jemersic et al., 2004; Maldonado et al., 2004; Wang et al., 2004; Motovski and Segales, 2004; Garkavenko et al., 2005). PMWS affects young weaner pigs and results in weight loss, tachypnea, dyspnea, enlarged lymph nodes and jaundice (Harding, 1998). PCV2 may also cause or contribute to other swine diseases such as congential tremors (CT) (Stevenson et al., 1999), porcine dermatitis and nephropathy syndrome (PDNS) (Rosell et al., 2000), reproductive failure (Meehan et al., 2001) and several other emerging ...
The present invention provides a novel chimeric porcine circovirus infectious DNA clone and live attenuated chimeric virus with the PCV2, preferably of subtype PCV2b, capsid gene integrated into a non
article{326621, abstract = {Stimulation of the porcine immune system causes increased replication of porcine circovirus 2 (PCV2) in vivo. In the present study, we investigated whether various cytokines (interleukin-1 [IL-1], IL-6, IL-10, tumor necrosis factor-alpha [TNF-alpha], interferon-alpha [IFN-alpha], and IFN-gamma) are able to influence PCV2 infection in vitro. No changes were observed in IL-1, IL-6, TNF-alpha, or IL-10-treated cells. However, it was demonstrated that IFN-alpha and IFN-gamma influenced PCV2 infection in porcine kidney cells (PK-15) and porcine monocytic cells (3D4/31). IFN-gamma added to the culture medium before, during, or after inoculation increased the number of PCV2 antigen-positive cells, respectively, by 418%, 171%, and 691% in PK-15 cells and by 706%, 114%, and 423% in 3D4/31 cells. IFN-alpha pretreatment decreased the number of infected PK-15 cells. When it was added after inoculation, IFN-alpha enhanced PCV2 infection by 529% in PK-15 cells and by 308% in 3D4/31 ...
The study conducts the first phylogenetic analysis of PCV2 in Chile. PCV2 isolated from Chilean intensive swine farms, covering over the 90% of the pork production, resulting a complete national perspective of PCV2. The results confirm the high presence of PCV2b (52%) and less represented PCV2a (14%). Surprisingly, 34% of sequences were PCV2a/PCV2d recombinant viruses. The no amplification of these 10 sequences by the selective PCR was further explained due to these primers were not able to amplified PCV2d, the major parenteral of these viruses. Furthermore, we were not able to detect PCV2d variant in the Chilean PCV2 diversity even if was the major parent of the recombinant viruses, probably this event occurred years before and the recombinant remained. PCV2a and PCV2b, and the recombinant viruses were found since 2005. Nevertheless, were not observed geographical, temporal and other patterns, most likely due to there is not enough sequences per farm.. Recently, a large-scale study determinates ...
1. National Center for Animal Health Surveillance. National Animal Health Monitoring Survey 2000. Available at: www.aphis.usda.gov/vs/ceah/cahm. Accessed January 12, 2006. 2. Halbur PG. Defining the causes of PRDC. Swine Consultant. 1996;fall:4-15. 3. Opriessnig T, Thacker EL, Yu S, Fenaux M, Meng X-J, Halbur P. Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneumoniae and porcine circovirus type 2. Vet Pathol. 2004;41:624-640. 4. Thacker EL, Halbur PG, Ross RF, Thanawongnuwech R, Thacker B. Mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. J Clin Microbiol. 1999;37:620-627. 5. Hsu FS, Yeh TP, Lee CT. Tiamulin feed medication for the maintenance of weight gains in the presence of mycoplasmal pneumonia in swine. J Anim Sci. 1983;57:1474-1478. 6. Hannan PC, Goodwin RF. Treatment of experimental enzootic pneumonia of the pig by norfloxacin or its 6-chloro analogue. Res ...
Porcine Circovirus 2 (PCV2) has been intriguing pig producers and scientists alike as it proved difficult to identify both the disease's origins and triggers. In this third episode of the series 'PCVD - From pathogenesis to protection', Pig Progress launches an attempt to unveil as many mysteries as possible that surround PCV2.
12.Dan Xu, Qian Du, Cong Han, Zengguo Wang, Xiujuan Zhang, Tongtong Wang, Xiaomin Zhao, Yong Huang, Dewen Tong*, p53 signaling modulation of cell cycle arrest and viral replication in porcine circovirus type 2 infection cells. Veterinary Research, 2016. 47(1): p. 120-131.. 13.Hongling Zhang, Yong Huang, Lili Wang, Tingting Yu, Zengguo Wang, Lingling Chang, Xiaomin Zhao, Xiaomao Luo, Liang Zhang, Dewen Tong*, Immortalization of porcine placental trophoblast cells through reconstitution of telomerase activity. Theriogenology, 2016. 85(8): p. 1446-1456.. 14.Delong Li, Yong Huang, Qian Du, Zhenyu Wang, Lingling Chang, Xiaomin Zhao, Dewen Tong*, CD40 Ligand and GMCSF coexpression enhance the immune responses and protective efficacy of PCV2 adenovirus vaccine. Viral Immunology, 2016. 29(3): p. 148-158.. 15.Xiaomin Zhao, Hailing Xiang, Xiaoyuan Bai, Naijiao Fei, Yong Huang, Xiangjun Song, Hongling Zhang, Liang Zhang, Dewen Tong*, Porcine parvovirus infection activates mitochondria-mediated apoptotic ...
Animal circoviruses belong to the Circovirus genus of the Circoviridae family. Nowadays, only swine and birds were identified as circovirus hosts. Circoviruses have a single-stranded circular genome of approximately 2000 nucleotide long. DNA of these viruses possesses : (i) a nonanucleotide sequence essential for replication, flanked by inverted repeat sequences, a palindrome that has the potential to form a stem-loop structure and (ii) two major ORFs, located on the viral and complementary strands, which encode respectively the replication-associated protein (Rep) and the capsid protein (Cap). All the circoviruses described at the present time, except porcine circovirus of type 1, are associated with immunosuppressive or immunodepressive diseases. Histopathological lesions such as cytoplasmic inclusions of virus in histiocytic cells and T and B lymphocyte depletion in lymphoid organs, are commonly noticed. No medical prophylaxis of circovirus infections is currently available.. ...
Combination vaccine gives swine industry effective, convenient option. FLORHAM PARK, N.J., Aug. 20, 2014 - Fostera™ PCV MH - the porcine circovirus (PCV) and Mycoplasma hyopneumoniae (M. hyo) combination vaccine that was built from the ground up by Zoetis - now has the longest demonstrated duration of immunity (DOI) to help protect pigs from porcine circovirus-associated disease (PCVAD) caused by PCV Type 2 (PCV2). The U.S. Department of Agriculture has granted the combination vaccine the extended label claim of at least 23 weeks of protection against PCV2, which is three weeks longer than any other PCV2 vaccine on the market.. "PCVAD continues to be one of the most economically harmful swine diseases. Affected pigs suffer from progressive weight loss, increased mortality and other clinical impacts, which takes a toll on producers productivity and profitability," said Darrell Neuberger, DVM, Pork Technical Services, Zoetis. "Now, with a vaccine that offers at least 23 weeks of immunity, ...
In 2016, two small-scale fattening pig farms (about 200 or 300 American Landrace pigs in total), locating in Guangdong province (southern China) and Jilin province (northern China) separated by more than 3, 000 km in distance (Fig. 1), suffered from severe respiratory disease (SRD). Pigs (2-3 months old) on both farms have received immunizations against PCV2, pseudorabies virus (PRV), classical swine fever virus (CSFV) and type 2 porcine reproductive and respiratory syndrome virus (PRRSV). However, the morbidity rate of pigs on the two farms was approximately 60%, the mortality in affected pigs was about 80%. From autopsy results of 18 individual dead pigs (n = 8 for farm A, n = 10 for farm B) (Additional file 1: Table S1), the similar macroscopic lesions were concentrated in the lungs and lymph nodes, which was characterized by PRRSV- or PCV2-like lesions including pulmonary interstitial widening, congestion and lymphadenopathy bleeding. These 18 pig lung samples were defined as the samples ...
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Background Diarrhea is one of the most common clinical symptoms reported in companion animal clinics. Dog circovirus (DogCV) is a new mammalian circovirus that is considered to be a cause of...
posted April 1, 2008 Boehringer Ingelheim Vetmedica Inc. again requests proposals for studies investigating porcine circovirus-associated
Read "Genetic characterization of type 2 porcine circoviruses detected in Hungarian wild boars, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Zoetis launched Fostera PCV MH on the US market. The vaccine is a one-bottle, one-dose combination vaccine that helps protect swine from Porcine Circovirus-associated disease (PCVAD) and enzootic pneumonia caused by Mycoplasma hyopneumoniae (M. hyo).
Vol 10: High yield production of pigeon circovirus capsid protein in the E. coli by evaluating the key parameters needed for protein expression.. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Ohio Ag Dept issued warning on Canine Circovirus, a contagious disease that has spread, killing 6 more dogs, but is there more to be feared than this virus …
Protection Against Evolving Virus. DURHAM, N.C., March 3, 2018 - Zoetis has introduced Fostera® Gold PCV MH, the U.S. pork industrys first vaccine to include two genotypes of porcine circovirus type 2 (PCV2) - 2a and 2b.. Together, the two genotypes provide the broadest range of protection against evolving PCV2 viruses in U.S. swine herds. The vaccines efficacy against 2d - the most prevalent PCV2 genotype - was demonstrated in a large field trial.. "Were pleased to introduce a vaccine that brings real value to the U.S. pork industry - one that provides broader protection against PCV2 genotypes and, more importantly, helps to minimize performance problems due to the virus, reduce monetary losses for producers and benefit animal welfare," said Lucina Galina, DVM, PhD, director, swine technical services, Zoetis.. The duration of immunity (DOI) with Fostera Gold PCV MH is at least 23 weeks for PCV and Mycoplasma hyopneumoniae (M. hyo), which means the vaccine will protect most herds through the ...
El present treball es centra en lestudi de la producció de proteïnes recombinants en línies cel·∙lulars de mamífer. Concretament, sha realitzat lestudi de tres estratègies de bioprocés, totes elles basades en el cultiu de cèl·∙lules HEK293. Com a proteïna model per a lexpressió de proteïnes heteròlogues sha triat la proteïna CapPCV2, la qual conforma la càpsida viral del Circovirus porcí serotip 2 (PCV2). Aquest virus és lagent causal de PCVDS (porcine circovirus diseases o malaties derivades de circovirus porcí). Aquest terme engloba un conjunt de malalties i síndromes que tenen un elevat impacte econòmic en la indústria porcina. El projecte sha enfocat des de la perspectiva de desenvolupament i optimització del bioprocés i, en conseqüència, lincrement de la producció volumètrica ha estat la força impulsora de tot el treball. En primer lloc es presenten els estudis per a la selecció del medi de cultiu i suplements nutricionals. El creixement cel·∙lular
Factors to be considered for deciding the optimal time of vaccination. Comparison of different vaccination programs in PCV2 subclinically infected piglets.
UK - Researchers at the Royal Veterinary College (RVC) have developed an oral vaccine against porcine circovirus type 2 (PCV2) based on the yeast Saccharomyces cerevisiae.
An experiment was conducted to determine the impact of increasing levels of Algamune ZPC (Algal Scientific Corporation, Plymouth, MI) on growth performance and porcine circovirus type 2 (PCV2)-specific immune response of nursery pigs housed under commercial conditions. Algamune ZPC is a polysaccharide-zinc complex feed additive composed of 35% β-1,3-glucan extracted from algae and 10% zinc. A total of 2,484 pigs (PIC 337 × 1050, initially 15.7 lb) were used in a 40-d trial. After feeding a common pelleted diet for 7 d after weaning, pigs were allotted to 1 of 6 dietary treatments in a randomized complete block design with 14 or 16 replicate pens and 27 pigs per pen. All pigs were vaccinated with PCV2 and M. hyopneumoniae vaccines (1 mL Fostera PCV and 1 mL Respisure- One; Zoetis, Florham Park, NJ) at d 3 after birth and at weaning. Blood samples of 72 pigs (12 pens per treatment) were collected on d 2, 18, and 38. The 6 experimental diets were fed in two phases (d 0 to 12 and 12 to 40). ...
Fast Shipping. Great Low Price. Circumvent PCV-M G2 for swine is used in the prevention of viremia, the reduction of virus shedding, the reduction of lymphoid infection caused by porcine circovirus Type 2 (PCV2), and in the reduction of lung lesions due to Mycoplasma hyopneumoniae. Circumvent PCV-M G2 is approved for piglets as early as 3 days of age. Convenient one or two dose administration options with five month PCV2 duration of immunity. Dosage Option 1: Give a single 2 ml dose IM to health
Average daily gain (ADG) and viral load (VL) were evaluated for pigs co-infected with porcine reproductive and respiratory syndrome (PRRS) virus (PRRSV) and porcine circovirus type 2b (PCV2b). Pigs were pre-selected for WUR genotype (a marker on chromosome 4 associated with weight gain and VL under PRRSV-challenge), half were vaccinated for PRRS, and half were not. Results indicate that vaccination for PRRS resulted in slower growth prior to co-infection and that the AB WUR genotype was associated with faster growth prior to co-infection, lower PRRS VL, and lower PCV2b VL in vaccinated pigs.
Detecting a Novel Circovirus in Foxes with Meningoencephalitis - How custom RNAscope® assay probes are enhancing viral understanding. The burden of infectious diseases on human health worldwide has markedly increased over the past few decades, most of these emerging events are caused by viruses originating from the animal world. Understanding the roots of viral behavior in animal hosts is therefore vital and this is a primary focus of Sarah Getus and Saskia Smitss work at the Department of Viroscience, Erasmus MC. In this interview, they explain how the creation of a custom RNAscope® in situ hybridization (ISH) probe allowed them to localize a newly discovered circovirus to neurologic lesions in foxes, providing valuable information to support causation.. Download PDF ...
En trece cap tulos se revisan las principales enfermedades que afectan al ganado porcino, tanto en producci n intensiva como extensiva. Realizado grandes especialistas en enfermedades infecciosas del ganado porcino de Espa a
Many vaccines currently given to humans are produced using animal products. This includes the use of media formulations supplemented with bovine serum. The use of animal products in vaccine production carries the risk of introducing infectious agents. While bovine serum is effective in supporting the growth of vaccine production cell lines such as Vero, there are many problems and risks from its use. Bovine serum has the potential to carry prions (mad cow disease). Recently, preparations of Rota Teq™ were found to be contaminated with porcine circovirus (1, bottom)-presumably from the use of animal products during production. To reduce the risk of transmission of infectious agents, irradiated serum is used for vaccine production; however, this adds additional costs. In addition to the safety risks, serum is also undefined, variable in performance from lot-to-lot, and can be a non-human contaminant in vaccine preparations (1, 2).. Current commercial vaccine media do not require the use of ...
Across the industry, the issue of neonatal diarrhea does not raise concerns to the level of porcine reproductive and respiratory syndrome or circovirus. But it is certainly a frustrating problem that farms deal with occasionally. The severity of baby pig scours varies widely across farms in terms of age at onset, duration, mortality and morbidity. The pathogen or cause is responsible for most of the
Porcine diseases associated with porcine circovirus 2 (PCV-2) infection have resulted in significant economic losses worldwide. A real-time recombinase polymerase amplification (RPA) assay was develop
Whats your diagnosis #3 Porcine Multisystemic Wasting Syndrome - PCV-2 See original post here History: A feeder pig was found dead in the morning, it was thinner than its penmates. Gross Necropsy: (no photos) The lungs were loosely adhered to the thoracic body wall by multiple strands of fibrin. The lungs were diffusely edematous and firm. The…
Read "Increased yield of porcine circovirus-2 by a combined treatment of PK-15 cells with interferon-gamma and inhibitors of endosomal-lysosomal system acidification, Archives of Virology" on DeepDyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
A blinded interlaboratory assessment of the diagnostic agreement and accuracy of serologic tests for routine detection of antibodies against Porcine circovirus-2 (PCV-2), including indirect fluorescent antibody tests (IFATs) and enzyme-linked immunosorbent assays (ELISAs) was conducted in 7 North American laboratories.
TY - JOUR. T1 - Particles resembling circovirus in the bursa of Fabricius of pigeons.. AU - Shivaprasad, H L. AU - Chin, Richard P. AU - Jeffrey, J. S.. AU - Latimer, K. S.. AU - Nordhausen, R. W.. AU - Niagro, F. D.. AU - Campagnoli, R. P.. PY - 1994/7. Y1 - 1994/7. N2 - Histological examination of the bursae from 12 pigeons under 4 months old revealed basophilic globular inclusion bodies, 5 to 25 microns in diameter, in the cytoplasm and the nuclei of the various bursal follicular cells. Electron microscopy of these inclusions revealed large electron-dense areas containing non-enveloped icosahedral viral particles, 14-19 nm in diameter, either loosely arranged or in paracrystalline array. Similar basophilic globular inclusion bodies were seen in the spleen and cecal tonsils of a few pigeons and in the duodenum of one pigeon. There were various degrees of lymphoid depletion in the bursa, spleen, and bone marrow. The morphology of the inclusions in the bursa and size of the viral particles are ...
Synonyms for AIDS wasting syndrome in Free Thesaurus. Antonyms for AIDS wasting syndrome. 1 synonym for AIDS: acquired immune deficiency syndrome. What are synonyms for AIDS wasting syndrome?
Con la intención de unificar la terminología para referirse a cada una de estas enfermedades y al conjunto de ellas, se ha propuesto recientemente el término PCVD para referirse al conjunto de enfermedades asociadas a PCV2, y la terminología que figura en la tabla para referirse a cada una de las PCVD (Segalés, 2012). En cuanto a la enfermedad reproductiva por PCV2, cabe añadir que recientemente se ha vinculado PCV2 a SMEDI (S-stillbirth-nacidos muertos, M-mummification-momificación, ED-embryonic death-muerte embrionaria e I-infertility-infertilidad) en primíparas (Meyns y col., 2012). Otra enfermedad que todavía hoy se sigue asociando al virus es el síndrome de dermatitis y nefropatía porcino, a pesar de considerarse una enfermedad mediada por immunocomplejos de la que no se ha demostrado claramente su etiología (Allan y col., 2000a; Rosell y col., 2000; Wellenberg y col., 2004). En los últimos años se está considerando también el impacto de la infección subclínica de los ...
Both Rotarix and RotaTeq are designed to protect infants from gastrointestinal illness caused by rotavirus, which kills more than 500,000 infants each year, mostly in low- and middle-income countries. Deaths are rare in the United States, but illness can require a hospital stay due to severe diarrhea. The oral vaccines are typically given to infants between two and six months of age in a two- or three-dose series.. * Read: Ensure Children Receive Vaccinations Before School Starts. An advisory panel for the FDA found that the risk to humans from the porcine (pig) circovirus type 1, or PCV1, was theoretical. Millions of doses of the vaccines have been given to infants with no sign of significant safety problems and PCV1 is not even known to cause health problems in pigs.. Another strain of the virus, PCV2, is believed to cause a wasting syndrome in young piglets, marked by diarrhea and an inability to gain weight, but it is not known to injure humans. Only PCV1 was detected in the Rotarix vaccine, ...
Recent findings by Professor of Cell and Developmental Biology David Bilder and his lab could lead to the development of much-needed targeted therapies for wasting syndrome in cancer patients (this syndrome is called cachexia, where muscle and fat tissues are destroyed throughout the body). Read more...
The Report Swine Diseases Treatment Market - Global Industry Trend Analysis 2012 to 2017 and Forecast 2017 - 2025 provides information on pricing, market analysis, shares, forecast, and company profiles for key industry participants. - MarketResearchReports.biz
The CDC National Center for HIV, STD, and TB Prevention provides the following information as a public service only. Providing synopses of key scientific ...
The AVMA House of Delegates is set to consider a resolution to increase member dues in 2011 so that the Association can continue to provide
A third of cancer patients die from a wasting syndrome. With new hints, researchers are closing in on what causes it and how to slow it down in order to give cancer patients more time to fight.
Introduction Real-time PCR tests have been developed to detect and quantify Porcine Circovirus type 2 (PCV2) and Lawsonia intracellularis in pigs faeces. Pooling of individual faecal samples is often used to reduce the costs of diagnostic testing. The objective of this study was to evaluate any change in the test sensitivity of PCV2 and L. intracellularis real-time PCR when individual faecal samples were pooled. Materials and Methods Forty eight faecal samples were collected from the rectum of individual pigs (,10 weeks) from four farms. Faecal samples were classified as diarrhoea +/- based on subjective assessment of consistency. Three individual samples were combined to make 16 pooled samples (8 diarrhoea; 8 non-diarrhoea). Individual and pooled samples were tested using real-time PCR tests specific for PCV2 and L. intracellularis. A positive result in any of the three individual samples was deemed "group positive". Changes in test sensitivity after combining the three individual samples were ...
E conomical loss produced by PMWS (Postweaning multisystemic wasting syndrome) is one of the most important issues that concern the pig industry. PMWS is cons...
EU - MSD Animal Health says the European Commission has approved its PORCILIS® PCV M Hyo vaccine that protects piglets against Porcine Circovirus and Mycoplasma hyopneumoniae infections.
See how medical marijuana could help relieve your wasting syndrome symptoms. Find patient reviews on local doctors and information on treatment options.
Activist Post. Startling reports of a new deadly virus infecting dogs have arisen in the media in the past month. Its said to be highly contagious for dogs and can kill them in just a few days. Currently, theres no viable veterinary treatment available, because no one even knows entirely what this is yet or how it spreads. Tests for it take weeks to get results, to no avail for the pet who has it.. Strangely, it is confined to three states - California, Ohio and Michigan. It was actually first detected in dogs in 2012. Although, media reports such as one coming out of Arizona seem confident that it will make its way across the United States, undoubtedly causing panic for dog lovers everywhere. Symptoms include severe intestinal inflammation, varying lethargy, weight loss, diarrhea and vomiting.. For now, it is being labeled in dogs as circovirus which is actually a name to describe the largely unknown small viruses seen in other species. It is suspected that the infected dogs have serious ...
1118DNAArtificial SequenceThis is a modified Kozaks sequence. 1ccgccatg 826DNAArtificial SequenceThis is a recombinant Eco R1 sequence. 2gaattc 63713DNAPorcine circovirus 3cagctatgac gtatccaagg aggcgttacc gcagaagaag acaccgcccc cgcagccatc 60ttggccagat cctccgccgc cgcccctggc tcgtccaccc ccgccaccgc taccgttgga 120gaaggaaaaa tggcatcttc aacacccgcc tctcccgcac cttcggatat actgtggaga 180aggaaaaatg gcatcttcaa cacccgcctc tcccgcacct tcggatatac tgtgacgact 240ttgttccccc gggagggggg accaacaaaa tctctatacc ctttgaatac tacagaataa 300gaaaggttaa ggttgaattc tggccctgct cccccatcac ccagggtgat aggggagtgg 360gctccactgc tgttattcta gatgataact ttgtaacaaa ggccacagcc ctaacctatg 420acccatatgt aaactactcc tcccgccata caatccccca acccttctcc taccactccc 480gttacttcac acccaaacct gttcttgact ccactattga ttacttccaa ccaaataaca 540aaaggaatca gctttggctg aggctacaaa cctctagaaa tgtggaccac gtaggcctcg 600gcactgcgtt cgaaaacagt aaatacgacc aggactacaa tatccgtgta accatgtatg 660tacaattcag agaatttaat cttaaagacc ccccacttaa accctaaatg aat 7134713DNAPorcine ...
We have the newest information! Why are sea stars growing sores, losing their arms, and dying within a few days? Over the last couple of years, sea stars along much of the Pacific coast of North America have been experiencing… ...
In patients age 19-64 with a high risk condition (see CDC site below), ACIP has long recommended they receive PCV 23, followed by a 2nd dose 5 years later. PCV 23 is also recommended as a one-time dose for all patients age 65 and over. Now the CDC is recommending, for patients age 19-64 with a high risk conditions, a dose of PCV 13, followed by PCV 23 at least 8 weeks later. For those high risk and in this age group who have already received PCV 23, they should also receive PCV 13 at least 1 year after their dose of PCV 23. Full recommendations are available at the CDC site (CDC site). ...
H1N1 Symptoms are akin to signs or symptoms of other epidemic viruses. These incorporate fever, cough, headaches, chills, entire body aches, a sore throat ...
This paper examines past occurrences in North America relevant to the possibility of biological disasters with animal origins. With respect to naturally occurring animal disease outbreaks, North America, while not as adversely affected by epizootics as other regions, has had its fair share of such outbreaks of both traditional and emerging animal diseases. The traditional category includes such diseases as anthrax, classical swine fever, bluetongue, brucellosis, foot and mouth disease, and the family of equine encephalomyelitis viruses. The emerging diseases include relatively more recent culprits such as postweaning multisystemic wasting syndrome, poultry enteritis mortality syndrome, and newly discovered examples of the transmissible spongiform encephalopathies. Additionally, several serious diseases of human beings that involve animal vectors or reservoirs occur naturally in North America or have emerged in recent decades; these include plague, hantavirus, monkeypox, West Nile virus and ...
The aim of this study was to identify the causative factors of porcine ear necrosis syndrome (PENS) in 72 pigs, 5.5-10 weeks in age housed on nine farms. Biopsy samples of ear pinnae were collected from all piglets for bacteriology, histopathology and in situ hybridization for porcine circovirus type 2 (PCV2). At the same time, serum samples were taken for serological analysis and viral PCR, and feed was sampled for mycotoxin analysis. The initial lesion of PENS seemed to be a focal epidermal necrosis. Streptococci were isolated from 44 and staphylococci from 36 pinnae. PCV2 could not be detected by in situ hybridization or qPCR. Seven piglets were positive for porcine reproductive and respiratory syndrome virus, and one for Mycoplasma suis. One piglet had antibodies against Sarcoptes scabiei var. suis. No infectious agents were found in 15 samples. Positive virology and parasitology were often found alongside positive bacteriology. Deoxynivalenol, zearalenone and ergot alkaloids were detected in feed.
Harmon KM, Gauger PC, Zhang J, Piñeyro PE, Dunn DD, Chriswell AJ. 2015. Whole-genome sequences of novel porcine circovirus type 2 viruses detected in swine from Mexico and the United States. Genome Announc 3(6):e01315-15. doi:10.1128/genomeA.01315-15.. Thomas JT, Chen Q, Gauger PC, Giménez-Lirola LG, Sinha A, Harmon KM, et al. 2015. Effect of Porcine Epidemic Diarrhea Virus Infectious Doses on Infection Outcomes in Naïve Conventional Neonatal and Weaned Pigs. PLoS ONE 10(10): e0139266.. Sinha A, Gauger P, Zhang J, Yoon KJ, Harmon K. 2015. PCR-based retrospective evaluation of diagnostic samples for emergence of porcine deltacoronavirus in US swine. Vet Microbiol Sep 30: 179(3-4).. Pepin, B. J., Kittawornrat, A., Liu, F., Gauger, P. C., Harmon, K., Abate, S., Main, R., Garton, C., Hargrove, J., Rademacher, C., Ramirez, A. and Zimmerman, J. (2015), Comparison of Specimens for Detection of Porcine Reproductive and Respiratory Syndrome Virus Infection in Boar Studs. Transboundary and Emerging ...
A little over two years ago, we commented on a general rise in the frequency of Actinobacillus suis (A. suis) isolation from our swine tissue submission cases, and wondered if it was related to the increased porcine circovirus type 2 (PCV2) activity (National Hog Farmer North American Preview, June 6, 2008).
Development of the first conventional and real-time polymerase chain reaction (PCR) tests for the diagnoses of duck circovirus (DuCV) infections is described. Both tests amplified a 230 bp fragment specific to the DuCV Rep gene. Although both tests had the same detection limit (13 x 10(3) target DNA/ml) when the target DNA was diluted in water, the detection limit of the real-time test (13 x 10(4 ...
Provides information on Pig Health, Swine Diseases, Hog Welfare and Pork Safety. Has nearly 1000 articles and links on the subject matter and has a book shop on pig topics. ...
Find all PCV Labs in Pataudi,Gurgaon. Now View Prices, Normal Range, Sample Results & Book Online for PCV Labs in Pataudi Gurgaon only on | Practo
Porcine circovirus type 2 (PCV2), Mycoplasma hyopneumoniae and Lawsonia intracellularis are economically important pathogens in modern pig breeding and rearing, worldwide. PCV2 infection can be associated with a wide range of diseases, including post-weaning multisystemic wasting syndrome (PMWS), abortion and reproductive failure, the porcine respiratory disease complex (PRDC) and porcine dermatitis and nephropathy syndrome (PDNS) (Allan and Ellis 2000). Porcine proliferative enteropathy, commonly referred to as ileitis, caused by L intracellularis, is a common and widespread intestinal infection in pigs. In its acute form, it can result in mortality in finishing pigs and young breeding stock, and in its chronic form, depression and unevenness of growth in growers and finishers (Lawson and Gebhart 2000). M hyopneumoniae is recognised as the causative agent of porcine enzootic pneumonia, a mild, chronic pneumonia commonly complicated by opportunistic infections with other bacteria (Maes and ...
The assembly and regulation of viral capsid proteins into highly ordered macromolecular complexes is essential for viral replication. Here, we utilize crystal structures of the capsid protein from the smallest and simplest known viruses capable of autonomously replicating in animal cells, circoviruses, to establish structural and mechanistic insights into capsid morphogenesis and regulation. The beak and feather disease virus, like many circoviruses, encode only two genes: a capsid protein and a replication initiation protein. The capsid protein forms distinct macromolecular assemblies during replication and here we elucidate these structures at high resolution, showing that these complexes reverse the exposure of the N-terminal arginine rich domain responsible for DNA binding and nuclear localization. We show that assembly of these complexes is regulated by single-stranded DNA (ssDNA), and provide a structural basis of capsid assembly around single-stranded DNA, highlighting novel binding ...
Facets of a Multisystemic Neuromuscular Disease: A Continuum of Perspectives. Abbreviations. References. References. Slideshow 2794486 by arnie
Oral fluid analysis for herd monitoring is of interest to the commercial pig production in Korea. The aim of this study was to investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. Samples collected by a rope-chewing method underwent reverse-transcriptase polymerase chain reaction (RT-PCR) or standard polymerase chain reaction (PCR) analysis, depending on the microorganism. Pathogens were divided into virus and bacteria groups. The former consisted of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 (PCV2), and the latter Pasteurella multocida, Haemophilus parasuis, Actinobacillus pleuropneumoniae, Mycoplasma hyopneumoniae (MHP), Mycoplasma hyorhinis, and Streptococcus suis (SS). All pathogens were detected more than once by PCR. Age-based analysis showed the PCR-positive rate increased with increasing age for PCV2 ...
Title: Phylogenetic analysis of beak and feather disease virus across a host ring-species complex. Authors: Eastwood, Justin R.; Berg, Mathew L.; Ribot, Raoul F. H.; Raidal, Shane R.; Buchanan, Katherine L.; Walder, Ken R.; Bennett, Andrew T. D. Source: Proceedings of the National Academy of Sciences of the USA, vol. 111, no. 39, page(s) 14153-14158, September 2014. Brief…
FIG. 1. (A) Histological pattern of a superficial inguinal lymph node of a healthy pig. Follicular and interfollicular areas are clearly differentiated. (B) Moderate lymphocyte depletion (score 2) in a superficial inguinal lymph node of a pig with PMWS. Follicles are still perceptible, but cellular depletion is evident in all the lymphoid parenchyma. (C) Severe lymphocyte depletion (score 3) in a superficial inguinal lymph node of a pig with PMWS. No follicles or medulla-like tissues are evident in the lymphoid tissue. HE staining was used for all panels. Magnification, ×40. ...
Virus infections possess persistent health challenges in swine industry leading to severe economic losses worldwide. The economic burden caused by virus infections such as Porcine Reproductive and Respiratory Syndrome Virus, Swine influenza virus, Porcine Epidemic Diarrhea Virus, Porcine Circovirus 2, Foot and Mouth Disease Virus and many others are associated with severe morbidity, mortality, loss of production, trade restrictions and investments in control and prevention practices. Pigs can also have a role in zoonotic transmission of some viral infections to humans. Inactivated and modified-live virus vaccines are available against porcine viral infections with variable efficacy under field conditions. Thus, improvements over existing vaccines are necessary to: (1) Increase the breadth of protection against evolving viral strains and subtypes; (2) Control of emerging and re-emerging viruses; (3) Eradicate viruses localized in different geographic areas; and (4) Differentiate infected from vaccinated
a) The Department of Agriculture and Industries may establish, conduct and maintain a swine disease diagnostic laboratory for the purpose of diagnosing contagious, infectious and communicable diseases of swine owned by producers of such livestock in Alabama, and the amount appropriated and made available for such purpose in the general appropriations act shall be used and expended by the Department of Agriculture and Industries to establish, operate and conduct such a laboratory.. (b) Testing for swine disease may be performed at a swine disease diagnostic laboratory at a location in the State of Alabama to be designated by the State Board of Agriculture and Industries or as otherwise provided in Section 2-2-33.. ...
Regarding your promoter question: yes, but its hard. if you put it there specially to have it expressed, then obviously you have an active promoter (unless youre utterly stupid). But there are plasmids that do not have promoters at all: they are only meant to make the bacteria copy the gene, not to express it. if you have the name of your plasmid, you can usually check with the manufacturer, they will have a map that will tell you what your promoter is. if its a viral promoter then youre all set. If its not, you dont really know unless you search the literature for it. Generally speaking though, a promoter WILL express your gene - thats what a promoter does. Its just that a lot of promoters arent active all the time. For example, the lac promoter is not active if the bacterium doesnt have a CAP protein ...
Li L, Kapoor A, Slikas B, Bamidele OSoji, Wang C, Shaukat S, Masroor MAlam, Wilson ML, Ndjango J-BN, Peeters M, et al. Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. Journal of Virology. 2010 ;84(4):1674-82. ...
Li L, Kapoor A, Slikas B, Bamidele OSoji, Wang C, Shaukat S, Masroor MAlam, Wilson ML, Ndjango J-BN, Peeters M, et al. Multiple diverse circoviruses infect farm animals and are commonly found in human and chimpanzee feces. Journal of Virology. 2010 ;84(4):1674-82. ...
Find all PCV Labs in Gurgaon Sector 49,Gurgaon. Now View Prices, Normal Range, Sample Results & Book Online for PCV Labs in Gurgaon Sector 49 Gurgaon only on | Practo
Online Doctor Chat - Is PCV 13 vaccination necessary for a 8 month child? If so where to get it free of cost? Online doctor patient chat conversation by Dr. Chakravarthy Mazumdar
Dont wait till your failed PCV system causes oil leaks and engine damage. Get 1990 Dodge DW Pickup replacement valves and breathers at CARiD.
임상시험에서 나타난 이상반응 : 각 빈도 군 내에서, 바람직하지 않은 반응은 중대성 내림차순으로 제시되어 있다. 빈도는 다음과 같이 정의된다 : · 매우 흔함: ≥ 1/10, · 흔함: ≥ 1/100에서 <1/10 · 흔하지 않음: ≥ 1/1,000에서 <1/100, · 드묾: ≥ 1/10,000에서 <1/1,000 · 매우 드묾: <1/10,000, · 불명: (현재 데이터로 추정 불가). 2개월~23개월의 영유아 : Menveo를 투여한 2개월 된 영아 참가자 8,735명을 대상으로 필수접종 백신과의 병용투여 무작위배정 다기관 대조 임상 시험을 통해 멘비오의 4회 접종 계획에 대한 안전성을 평가하였다. 필수접종백신(DTaP, Hib, PCV백신)만 투여한 사람들과 멘비오와 필수접종백신을 동시에 투여받은 사람들과 비교하였을 때 전신 및 국소반응의 비율의 발생 비율의 차이가 없었다. 가장 흔한 국소반응은 주사부위 압통. 전신반응에 ...
Afrikaans: Beak and feather disease (BFD), wat veroorsaak word deur Beak and feather disease virus (BFDV) is n dermatologiese toestand wat papegaai spesies versmag. Dit word toenemend moeilik om nie net die beduidende negatiewe ekonomiese impak wat die virus het op die papegaai teling bedryf nie, maar ook die nadelige uitwerking wat dit op die voortbestaan van die endemiese Kaapse papegaai (Poicephalus robustus) te ignoreer. Die virus, n lid van die Circoviridae, is bekend as n nie-omvou, sirkulêre, enkelstring DNA genoom in besit te neem. Twee groot oop lees rame (OLRe) enkodeer die replikasie verwante proteïene (Rep) en die kapsied proteïen (KP). Die studie was uiteengesit om die huidige diagnostiese strategie vir BFDV te evalueer en te verbeter, met beide molekulêre en serologiese tegnieke. Die volgende doelwitte is gepoog: 1. Om polimerase kettingreaksie (PKR) en kwantitatiewe werklike tyd polimerase kettingreaksie (qPCR) as diagnostiese instrumente vir BFDV te vergelyk. Opsporing van ...
The detection of avian viruses in wild populations has considerable conservation implications. For DNA-based studies, feathers may be a convenient sample type for virus screening and are, therefore, an increasingly common technique. This is despite recent concerns about DNA quality, ethics, and a paucity of data comparing the reliability and sensitivity of feather sampling to other common sample types such as blood. Alternatively, skeletal muscle tissue may offer a convenient sample to collect from dead birds, which may reveal viraemia. Here, we describe a probe-based quantitative real-time PCR for the relative quantification of beak and feather disease virus (BFDV), a pathogen of serious conservation concern for parrots globally. We used this method to test for BFDV in wild crimson rosellas (Platycercus elegans), and compared three different sample types. We detected BFDV in samples from 29 out of 84 individuals (34.5%). However, feather samples provided discordant results concerning virus ...
Psittacine Beak and Feather disease is also known as Pittacine Circovirus disease. Psittacine Beak and Feather disease (PBFD) is a viral disease. All psittacine birds such as parrots, cockatoos and lorikeets are susceptible to the disease and it is believed that doves can be too.
Guy Regnanrd will present the MCB seminar with a talk entitled, Development of a potential challenge model and plant-produced vaccine candidate for beak and feather disease virus.. ...
Mycoplasma hyopneumoniae est lagent causal de la pneumonie enzootique. On le retrouve dans plusieurs élevages de porcs à travers le monde. Même si ce micro-organisme est présent dans plusieurs troupeaux canadiens, peu dinformations sont présentement disponibles sur les isolats québécois. Un total de 160 poumons de porcs possédant des lésions de pneumonie ont été récupérés à labattoir, mis en culture et testés par PCR pour M. hyopneumoniae et Mycoplasma hyorhinis. Dautres pathogènes bactériens communs du porc et les virus du syndrome reproducteur et respiratoire porcin (VSRRP), de linfluenza et le circovirus porcin de type 2 (CVP2) ont été également testés. Quatre-vingt-dix pourcent des échantillons étaient positifs pour M. hyopneumoniae et 5.6% létaient seulement pour M. hyorhinis. Dans ces échantillons positifs pour M. hyopneumoniae, la concentration de ce mycoplasme variait de 1.17 x 105 à 3.37 x 109 génomes/mL. Vingt-cinq poumons positifs en culture ou par ...
2005) A comparison of haemagglutination, haemagglutination inhibition and PCR for the detection of psittacine beak and feather disease virus infection and a comparison of isolates obtained from loriids. Journal of General Virology, 86 (11). pp. 3039-3046. Foster, D.J., Thoday, K.L., Arthur, J.R., Nicol, F., Beatty, J.A., Svendsen, C.K., Labuc, R., McConnell, M., Sharp, M., Thomas, J. and Beckett, G.J. ...
This study by Trupin and colleagues (2011) evaluated the ability of the Multisystemic Therapy-Family Integrated Transitions (MST-FIT) program to affect recidivism rates. The total sample size was 274 youths who were transitioning back to the community from one of five Washington State Juvenile Rehabilitation Administration (JRA) facilities between 2001 and 2005. The sample included 105 youths who participated in MST-FIT and served as the treatment group. The control group consisted of 169 eligible youths who did not participate in MST-FIT because they returned to counties where the program was unavailable; this group received treatment-as-usual JRA parole services. The average age of youth in the total sample was 16.35 years. Eighty-three percent of the sample were male; 70 percent were white, 15 percent were African American, 12 percent were Hispanic, and 4 percent were American Indian/Alaska Native ...
Adverse events appear to be dose-related and typically affect the central nervous system.6 The most common side effects include euphoria, paranoia, somnolence, and dizziness. Notably, caution should be used in patients with a history of substance abuse, as tolerance and dependence may develop with prolonged use. Dronabinol may also lower seizure threshold and may cause occasional hypotension. No major drug interactions are expected, though dronabinol is a minor substrate of the CYP2C9 and -3A4 pathways.22,23. Megestrol Acetate: Megestrol acetate was historically indicated only for breast cancer, until it was found that it had the unwelcome adverse reaction of weight gain.7 However, what is considered a side effect in one disease can be utilized as the desired therapeutic effect in another. Such is the case in wasting. Megestrol acetate has shown increases in total body weight of 4 kg to 5 kg over a period of 12 weeks.6 But while the drug is very effective in increasing total body weight, the ...
The Glässer disease is caused by a bacteria called |em|Haemophilus parasuis|/em|, which causes polyserositis and sporadic arthritis in piglets and growers.
Form-A-Feeds blog offers pertinent industry information, company updates, and other news. Read this article about VFD in the swine industry.
Cannabis sativa, a plant that contains the psychoactive ingredient delta-9 tetrahydrocannabinol (THC). Cannabis ingestion typically produces feelings of euphoria and/or drowsiness. Cannabis is used medically to reduce nausea due to chemotherapy and restore appetite in persons with wasting syndrome ...
Genus Circovirus. *Genus Cyclovirus. *Familie Geminiviridae (ssDNA(+/-): die einzelnen Gene haben unterschiedliche Polarität) ...
Ellis, J (2014). "Porcine circovirus: A historical perspective". Veterinary Pathology. 51 (2): 315-27. doi:10.1177/ ...
Cheung, A. K.; Ng, T. F.-f.; Lager, K. M.; Alt, D. P.; Delwart, E. L.; Pogranichniy, R. M. (2014). "Unique Circovirus-Like ... A virus - Boiling Springs Lake virus - appears to have evolved by a recombination event between a DNA virus (circovirus) and an ... Meehan BM, Creelan JL, McNulty MS, Todd D (1997). "Sequence of porcine circovirus DNA: affinities with plant circoviruses". J ... Rosario K, Duffy S, Breitbart M (2009). "Diverse circovirus-like genome architectures revealed by environmental metagenomics". ...
For example, Porcine circovirus type 1 has a genome of only 1759 nucleotides and a capsid diameter of only 17 nm. As a whole, ... Porcine circovirus 2 Archived July 4, 2007, at the Wayback Machine. K. Rosario; R.O. Schenck; R.C. Harbeitner; S.N. Lawler; M. ...
A circovirus-given the name raven circovirus or RaCV-was isolated from an Australian raven suffering from feather lesions in ... It has affinities with canary circovirus (CaCV) and pigeon circovirus (PiCV). Its clinical significance is unknown. Tick ... Stewart, Meredith E.; Perry, Ross; Raidal, Shane R. (2006). "Identification of a novel circovirus in Australian ravens (Corvus ...
... virus Canary circovirus Duck circovirus Finch circovirus Goose circovirus Gull circovirus Pigeon circovirus Porcine circovirus- ... 1 Porcine circovirus-2 Starling circovirus Swan circovirus Genus: Cyclovirus Chicken anemia virus The family Circoviridae ... Two yet-uncategorized circovirus-like viruses have been identified in fish- Barbel circovirus (BaCV) 1 and 2. Their genomes are ... Genus Circovirus: type species: Porcine circovirus 1 Genus Cyclovirus : type species Dragonfly associated cyclovirus 8, A ...
PRRS, Circovirus, and clostridial enteritis, among others, can be present in these systems. Crowding, mixing of groups, and ...
Examples of viruses carried by pigs include porcine herpesvirus, rotavirus, parvovirus, and circovirus. Of particular concern ...
Postweaning multisystemic wasting syndrome has been causally associated with porcine circovirus type 2. The Iotatorquevirus ...
It has a low level of homology to the Circovirus chicken anemia virus. The virus can be isolated from the blood and all the ...
... is a veterinary recombinant vaccine for porcine circovirus type 2 (PCV 2). It is manufactured by German ...
Examples of viruses carried by pigs include porcine herpesvirus, rotavirus, parvovirus, and circovirus. Porcine herpesviruses ... and rotaviruses can be eliminated from the donor pool by screening, however others (such as parvovirus and circovirus) may ...
... stated that there were indications that the pigs carried porcine circovirus. Porcine circovirus, he said, was spread among pigs ... The BBC reported with no proof that some pig corpses were infected by porcine circovirus. In early March news stories began to ... "No matter the strain, avian flu has never had any connection to porcine circovirus." However, the pigs themselves could serve ...
... the first human circovirus". J. Virol. 73: 3582-3586. "AABB website" (PDF). Retrieved December 25, 2017. Biagini, P.; Gallian, ...
The causative virus-beak and feather disease virus (BFDV)-belongs to the taxonomic genus Circovirus, family Circoviridae. It ... The virus was originally designated PCV (psittacine circovirus), but has since been renamed Beak and feather disease virus. ... The open reading frames have some homology to porcine circovirus (family Circoviridae), subterranean clover stunt virus and ... and in part to avoid confusion with Porcine circovirus, also called PCV. Detection A variety of tests for the presence of BFDV ...
Porcine epidemic diarrhea virus Porcine reproductive and respiratory syndrome virus Porcine circovirus Porcine parvovirus. ...
"Comparison of the structures of three circoviruses: Chicken anemia virus, porcine circovirus type 2, and beak and feather ...
... pigeon circovirus, canary circovirus goose circovirus. Herpesviruses are ubiquitous pathogens infecting animals and humans. ... There are to genera: gyrovirus, with one species called chicken anemia virus; and circovirus, which includes porcine circovirus ...
"Tracking viral evolution during a disease outbreak: the rapid and complete selective sweep of a circovirus in the endangered ...
On March 22, 2010, the detection of DNA from porcine circovirus types 1 and 2 within RotaTeq and Rotarix prompted the FDA to ... stating that porcine circovirus types 1 and 2 pose no safety risks in humans and concluded that health risks involved did not ... suspend the use of rotavirus vaccines while conducting an investigation the finding of DNA from porcine circovirus-1 (PCV1) in ...
A human Circovirus called TTV is included within this classification and is found in almost all humans, infecting them ...
PCVD is caused by porcine circovirus type 2 (PCV-2). The North American industry endorses "PCVAD" and European use "PCVD" to ... Porcine circoviral disease (PCVD) and Porcine circovirus associated disease (PCVAD), is a disease seen in domestic pigs. This ... Opriessnig, Tanja; Meng, Xiang-Jin; Halbur, Patrick G. (2007-11-01). "Porcine circovirus type 2 associated disease: update on ... Opriessnig, T; Langohr I (January 2013). "Current state of knowledge on porcine circovirus type 2-associated lesions". ...
... virus Canary circovirus Duck circovirus Finch circovirus Goose circovirus Gull circovirus Pigeon circovirus Porcine circovirus- ... 1 Porcine circovirus-2 Starling circovirus Swan circovirus Genus: Gyrovirus Chicken anemia virus Family: Geminiviridae Genus: ...
In addition to its direct causal role in reproductive failure, PPV can potentiate the effects of porcine circovirus type II ( ...
... leptospira QI09AL08 Porcine circovirus + mycoplasma QI09AM01 Escherichia antiserum QI09AM02 Pasteurella antiserum QI09AM03 ... virus QI09AA06 Classical swine fever virus QI09AA07 Porcine circovirus QI09AB01 Treponema QI09AB02 Escherichia QI09AB03 ...
porcine circovirus type 2, porcine circovirus type 2 vaccine, PCV2. Search the AASV web site for pages with similar keywords. ... Porcine circovirus type 2 (PCV2) is a small, non-enveloped, single-stranded DNA virus in the genus Circovirus within the family ... Comparación de las vacunas comerciales de subunidades de una dosis y dos dosis contra circovirus porcino tipo 2 expresado en ... Comparaison de vaccins sous-unitaires commerciaux une-dose et deux-doses contre le circovirus de type 2 exprimé dans un ...
In addition, a novel porcine circovirus from a case of a sow with dermatitis, nephropathy syndrome and reproductive failure has ... caused by porcine circovirus type 2 (PCV2), threaten the pig industry worldwide. Five genotypes of PCV2 were recently ... been identified based on metagenomic analysis and classified as porcine circovirus type 3 (PCV3). Therefore, the current study ... Porcine circovirus (PCV), belonging to the family Circoviridae and the genus Circovirus, is the smallest virus with a circular ...
PCVD is caused by porcine circovirus type 2 (PCV-2). The North American industry endorses "PCVAD" and European use "PCVD" to ... Porcine circoviral disease (PCVD) and Porcine circovirus associated disease (PCVAD), is a disease seen in domestic pigs. This ... Opriessnig, Tanja; Meng, Xiang-Jin; Halbur, Patrick G. (2007-11-01). "Porcine circovirus type 2 associated disease: update on ... Opriessnig, T; Langohr I (January 2013). "Current state of knowledge on porcine circovirus type 2-associated lesions". ...
(2009) Rosario et al. Journal of General Virology. Single-stranded DNA (ssDNA) viruses with circular genomes are the smallest viruses known to infect eukaryotes. The present study identified 10 novel genomes similar to ssDNA circoviruses through da...
... virus Canary circovirus Dog circovirus Duck circovirus Finch circovirus Goose circovirus Gull circovirus Pigeon circovirus ... Porcine circovirus-1 Porcine circovirus-2 Starling circovirus Swan circovirus Viruses in Circovirus are non-enveloped, with ... https://microbewiki.kenyon.edu/index.php/Circovirus#Virion_Structure_of_a_Circovirus Viralzone: Circovirus ICTV Circovirus - ... Circovirus is a genus of viruses, in the family Circoviridae. Birds (such as pigeons and ducks) and pigs serve as natural hosts ...
I suspect the problem could be circo virus, it can cause young pigeons to have a bad immunity. I also suspect circovirus could ... The circovirus itself doesnt make the bird ill, other infections do. In your case I suspect there could also have been kidney ... Dear Sir, I started breeding pigeons again after 10 years and it was all ok but in the middle of july i suffered from the CIRCO VIRUS ... Is there any adenovirus and herpesvirus vaccines available for pigeons? Can we use circovirus and pox vaccines for chickens? Is ...
General facts about dog circovirus (DogCV); diseases associated with this viral disease; signs, management and prognosis for ... Dogs infected with circovirus have been reported in California and circovirus may be associated with recent illness and death ... Circovirus in Tissues of Dogs with Vasculitis and Hemorrhage. *Genomic Characterization of a Circovirus Associated with Fatal ... Dog Circovirus. Canine enteritis can be caused by a number of viral, bacterial or parasitic agents. The most common viral ...
Beak and feather disease virus, Porcine circovirus 1, Porcine Circovirus 2, Canary circovirus, Goose circovirus, seven ... Reproduction Cycle of a Circovirus in a Host Cell. In case of the Porcine circovirus type 1 (PCV1), the rep gene directs the ... Virion Structure of a Circovirus. The virions of a circovirus consist of a capsid that is enveloped. Virions have an ... Circovirus. From MicrobeWiki, the student-edited microbiology resource. Revision as of 14:47, 29 June 2006 by Mschlemm. (talk ...
again requests proposals for studies investigating porcine circovirus-associated ... again requests proposals for studies investigating porcine circovirus-associated disease.. The company created the PCVAD ...
The Duck circovirus (DuCV) is a type of virus found in ducks. Strains of the virus have predominantly been found in China, ... Duck circovirus is a small nonenveloped virus with a monomeric single-stranded circular DNA genome. DuCV has been clustered in ... It was found that ducks between the ages of 40∼60 days were more susceptible to Duck circovirus. There was no evidence showing ... A vaccination against Duck circovirus has not been found. Researchers are still actively trying to find a vaccine.[citation ...
Canine circovirus (CaCV or DogCV), first isolated in 2012, is a small non-enveloped, icosahedral, single-stranded DNA virus ... CaCV (genome 1) was the first circovirus to be identified that infects a mammal species other than pigs. The genome of CaCV is ... "Circovirus in domestic and wild carnivores: An important opportunistic agent?". Virology. 490: 69-74. doi:10.1016/j.virol. ... "Circovirus in Tissues of Dogs with Vasculitis and Hemorrhage". Emerging Infectious Diseases. 19 (4): 534-541. doi:10.3201/ ...
There are 2 strains: type 1 and type 2. Porcine Circovirus Associated Disease is caused by porcine circovirus type 2 (PCV2). ... Porcine circovirus (PCV) is a single-stranded DNA virus (class II), that is nonenveloped with an unsegmented circular genome. ... He JL, Dai D, Zhou N, Zhou JY (2012) Analysis of Putative ORF3 Gene Within Porcine Circovirus Type 2. Hybridoma (Larchmt) 31(3 ... An independent academic research team in San Francisco identified porcine circovirus-1 DNA in two lots of Rotarix, and follow- ...
What exactly is circovirus, and what steps can you take to ensure your dog doesnt catch it? This article debunks the myths. ... What exactly is circovirus, and what steps can you take to ensure your dog doesnt "catch" it?. Many people are worried about ... One difference with circovirus is that it may cause vascular problems, so veterinarians do need to be aware of that potential. ... When looked for in one study, Circovirus was found in 19 of 198 dogs with diarrhea, and 14 of 204 dogs. Most stools had ...
Rabbit polyclonal Porcine circovirus antibody. Validated in WB, IHC. Independently reviewed in 1 review(s). Immunogen ... Primary - Rabbit Anti-Porcine circovirus antibody (ab183908) IHC-P, WB Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718) ... Porcine Circovirus (PCV) is a single stranded DNA virus (class II), that is non-enveloped with an un-segmented circular genome ... Anti-Porcine circovirus antibody (ab183908) at 1/1000 dilution + Lysate from Sf21 cells, expressing recombinant PCV2 protein by ...
The present invention provides a novel chimeric porcine circovirus infectious DNA clone and live attenuated chimeric virus with ... Porcine Circovirus Type 2 and Porcine Circovirus-Associated Disease, J Vet Intern Med). Observation of severe clinical PCVAD in ... Fine mapping of antigenic epitopes on capsid proteins of porcine circovirus, and antigenic phenotype of porcine circovirus type ... Porcine circovirus (PCV) is a small, non-enveloped DNA virus which belongs to the family Circoviridae (Todd, D. et al., 2005, ...
The term porcine circovirus-associated disease (PCVAD) is currently used to refer to any of these diseases when they are ... Porcine circovirus 2 (PCV2) is currently considered an important etiologic agent of swine and its infection has potentially ... "Fine Mapping of Antigenic Epitopes on Capsid Proteins of Porcine Circovirus and Antigenic Phenotype of Porcine Circovirus Type ... The term porcine circovirus-associated disease (PCVAD) is currently used to refer to any of these diseases when they are ...
... yesterday said it has detected very low levels of DNA from porcine circovirus (PCV) in its Rotateq vaccine, making it the ... yesterday said it has detected very low levels of DNA from porcine circovirus (PCV) in its Rotateq vaccine, making it the ...
Reproduction Cycle of a Circovirus in a Host Cell== In case of the Porcine circovirus type 1 (PCV1), the rep gene directs the ... Virion Structure of a Circovirus== The virions of a circovirus consist of a capsid that is enveloped. Virions have an ... Canary circovirus, Goose circovirus ==Description and Significance== Circoviruses are very small non-enveloped icosahedral ... Genome Structure== The genome of the circovirus is monomeric. It is unsegmented and contains a single molecule of circular, ...
They have finally isolated the virus , named circo virus. It comes from pigs and birds. The similarities to avian and swine flu ... H1N1 , Bird Flu, and Circo virus in dogs? page: 1 #liveFeed1, #liveFeed2, #liveFeed3 { margin: 40px 2px 50px 4px; width: 280px ... which has been identified as circo virus. ( new virus in dogs) My mind wonders, with the flu vaccine, could this be being ...
CANADA - Porcine Circovirus-2 (PCV-2) has become a major health concern not only in the United States, but in other world ...
J. Segalés, G. M. Allan, and M. Domingo, "Porcine circovirus diseases," Animal Health Research Reviews, vol. 6, no. 2, pp. 119- ... Figure 1: Detection of porcine circovirus type 2 (PCV2) with PCR method. The primers designed on the basis of the ORF2 of the ... S. P. Chen, Y. J. Weng, C. P. Tsai et al., "Identification of pathogenic porcine circovirus in pigs with post-weaning ... J. Kim, H.-K. Chung, and C. Chae, "Association of porcine circovirus 2 with porcine respiratory disease complex," Veterinary ...
J. Segalés, G. M. Allan, and M. Domingo, "Porcine circovirus diseases," Animal Health Research Reviews, vol. 6, no. 2, pp. 119- ... S. P. Chen, Y. J. Weng, C. P. Tsai et al., "Identification of pathogenic porcine circovirus in pigs with post-weaning ... J. Kim, H.-K. Chung, and C. Chae, "Association of porcine circovirus 2 with porcine respiratory disease complex," Veterinary ... S. Cao, H. Chen, J. Zhao et al., "Detection of porcine circovirus type 2, porcine parvovirus and porcine pseudorabies virus ...
Raven circovirus (RaCV), DQ146997; canary circovirus (CaCV), AJ301633); Columbid circovirus (CoCV), AF252610; Gull circovirus ( ... Goose circovirus (GoCV), AJ304456; Duck circovirus (DuCV), DQ100076; Porcine circovirus 1 (PCV1), AY660574; PCV2, AY424401; ... A) Genome organization of dog circovirus (DogCV) and porcine circovirus 2 (PCV2). B) Phylogenetic analysis of DogCV strains ( ... GenBank accession numbers for circoviruses used in the analysis: Finch circovirus (FiCV), DQ845075; Starling circovirus (StCV ...
... discusses the emerging canine circovirus (DogCV). This talk was part of the Maddies® Shelter Medicine Conference track of the ...
Porcine circovirus 2 (PCV2) replicates in vivo in a wide variety of cells, including cells of the monocyte /macrophage lineage ...
  • One- and two-dose recombinant porcine circovirus type 2 (PCV2) vaccines did not differ significantly in terms of immunological testing (neutralizing antibody titers, number of interferon-γ-secreting cells), virological testing (number of PCV2 genomic copies per mL serum), and pathological evidence of infection (lymphoid lesions scores and PCV2 antigen-positive cells). (aasv.org)
  • Des vaccins recombinants une- et deux-doses contre le circovirus porcin de type 2 (PCV2) ne différaient pas significativement en terme de réponse immunologique (titres d'anticorps neutralisant, nombre de cellules secrétant de l'interféron-γ), d'analyses virologiques (nombre de copies du génome de PCV2 par mL de sérum), et d'évidence pathologique de l'infection (pointage des lésions lymphoïdes et cellules positives pour l'antigène PCV2). (aasv.org)
  • Las vacunas de una y dos dosis del circovirus porcino tipo 2 recombinante (PCV2 por sus siglas en inglés) no difirieron significativamente en términos de pruebas inmunológicas (títulos de anticuerpos neutralizantes, número de células secretoras de interferón-γ), pruebas virológicas (número de copias genómicas del PCV2 por mL de suero), y evidencia patológica de infección (puntajes de lesiones linfoides y células positivas al antígeno de PCV2). (aasv.org)
  • In case of the Porcine circovirus type 1 (PCV1), the rep gene directs the synthesis of two proteins. (kenyon.edu)
  • source: [http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/00.016.0.01.htm ICTV dB Descriptions]) ==Reproduction Cycle of a Circovirus in a Host Cell== In case of the Porcine circovirus type 1 (PCV1), the rep gene directs the synthesis of two proteins. (kenyon.edu)
  • The replication proteins Rep and Rep' of porcine circovirus type 1 (PCV1) are both capable of introducing and resealing strand discontinuities at the viral origin of DNA replication in vitro underlying genome amplification by rolling-circle replication. (nih.gov)
  • More recently, a dog circovirus (DogCV) was detected in dogs with vasculitis and/or hemorrhagic diarrhoea in the US. (gopetsamerica.com)
  • DogCV shows many similarities to the porcine circovirus but it is not the same. (gopetsamerica.com)
  • We characterized the complete genome of a novel dog circovirus (DogCV) from the liver of a dog with severe hemorrhagic gastroenteritis, vasculitis, and granulomatous lymphadenitis. (nih.gov)
  • Organ tissues from sentinel dog (dog 1) and 2 other dogs (dogs 2 and 3) that were positive by in situ hybridization (ISH) analysis for dog circovirus (DogCV). (nih.gov)
  • High amino acid sequence similarity was demonstrated between a potential 33.3-kDa protein product of ORF1 of BFDV and the replicase-associated protein of porcine circovirus (PCV), subterranean clover stunt virus, and faba bean necrotic yellows virus. (nih.gov)
  • Li D, Wang J, Xu S, Cai S, Ao C, Fang L, Xiao S, Chen H, Jiang Y (2018) Identification and functional analysis of the novel ORF6 protein of porcine circovirus type 2 in vitro. (springer.com)
  • The morphology of the inclusions in the bursa and size of the viral particles are most consistent with circovirus. (elsevier.com)
  • With reports of infected flocks in northern and southern California, Canada, and Australia, it now appears that Pigeon Circovirus may become a major problem among mixed-aged flocks. (albertaclassic.com)
  • Psittacine circovirus (PCV) has been identified in more than 60 psittacine species worldwide in both aviary and wild populations. (massey.ac.nz)