A mitosporic Onygenaceae fungal genus which causes adiaspiromycosis, a pulmonary mycosis of man and rodents. One of its teleomorphs is Ajellomyces.
A genus of fungi in the family Corticiaceae, order Stereales, that degrades lignin. The white-rot fungus Phanerochaete chrysosporium is a frequently used species in research.
A phylum of fungi that produce their sexual spores (basidiospores) on the outside of the basidium. It includes forms commonly known as mushrooms, boletes, puffballs, earthstars, stinkhorns, bird's-nest fungi, jelly fungi, bracket or shelf fungi, and rust and smut fungi.
The most abundant natural aromatic organic polymer found in all vascular plants. Lignin together with cellulose and hemicellulose are the major cell wall components of the fibers of all wood and grass species. Lignin is composed of coniferyl, p-coumaryl, and sinapyl alcohols in varying ratios in different plant species. (From Merck Index, 11th ed)
Reversibly catalyze the oxidation of a hydroxyl group of carbohydrates to form a keto sugar, aldehyde or lactone. Any acceptor except molecular oxygen is permitted. Includes EC 1.1.1.; EC 1.1.2.; and 1.1.99.
Alcohols derived from the aryl radical (C6H5CH2-) and defined by C6H5CHOH. The concept includes derivatives with any substituents on the benzene ring.
Elimination of ENVIRONMENTAL POLLUTANTS; PESTICIDES and other waste using living organisms, usually involving intervention of environmental or sanitation engineers.
A family of bracket fungi, order POLYPORALES, living in decaying plant matter and timber.
A mitosporic Ophiostomataceae fungal genus, whose species Sporothrix schenckii is a well-known animal pathogen. The conidia of this soil fungus may be inhaled causing a primary lung infection, or may infect independently via skin punctures.
An exocellulase with specificity for the hydrolysis of 1,4-beta-D-glucosidic linkages in CELLULOSE and cellotetraose. It catalyzes the hydrolysis of terminal non-reducing ends of beta-D-glucosides with release of CELLOBIOSE.
A disaccharide consisting of two glucose units in beta (1-4) glycosidic linkage. Obtained from the partial hydrolysis of cellulose.
A 2,4,6-trinitrotoluene, which is an explosive chemical that can cause skin irritation and other toxic consequences.
An extensive order of basidiomycetous fungi whose fruiting bodies are commonly called mushrooms.
A family of fungi, order POLYPORALES, found on decaying wood.
A polysaccharide with glucose units linked as in CELLOBIOSE. It is the chief constituent of plant fibers, cotton being the purest natural form of the substance. As a raw material, it forms the basis for many derivatives used in chromatography, ion exchange materials, explosives manufacturing, and pharmaceutical preparations.
Phenols substituted with one or more chlorine atoms in any position.
A kingdom of eukaryotic, heterotrophic organisms that live parasitically as saprobes, including MUSHROOMS; YEASTS; smuts, molds, etc. They reproduce either sexually or asexually, and have life cycles that range from simple to complex. Filamentous fungi, commonly known as molds, refer to those that grow as multicellular colonies.
An insecticide and herbicide that has also been used as a wood preservative. Pentachlorphenol is a widespread environmental pollutant. Both chronic and acute pentachlorophenol poisoning are medical concerns. The range of its biological actions is still being actively explored, but it is clearly a potent enzyme inhibitor and has been used as such as an experimental tool.
A product of hard secondary xylem composed of CELLULOSE, hemicellulose, and LIGNANS, that is under the bark of trees and shrubs. It is used in construction and as a source of CHARCOAL and many other products.
An insecticide synergist, especially for pyrethroids and ROTENONE.
An organochlorine insecticide.
An endocellulase with specificity for the hydrolysis of 1,4-beta-glucosidic linkages in CELLULOSE, lichenin, and cereal beta-glucans.
An organochlorine insecticide that is slightly irritating to the skin. (From Merck Index, 11th ed, p482)
Proteins found in any species of fungus.
Deoxyribonucleic acid that makes up the genetic material of fungi.
Oxidases that specifically introduce DIOXYGEN-derived oxygen atoms into a variety of organic molecules.
The functional hereditary units of FUNGI.
A highly poisonous substance that was formerly used as an insecticide. The manufacture and use has been discontinued in the U.S. (From Merck Index, 11th ed)
Substances which pollute the soil. Use for soil pollutants in general or for which there is no specific heading.
A trace element with atomic symbol Mn, atomic number 25, and atomic weight 54.94. It is concentrated in cell mitochondria, mostly in the pituitary gland, liver, pancreas, kidney, and bone, influences the synthesis of mucopolysaccharides, stimulates hepatic synthesis of cholesterol and fatty acids, and is a cofactor in many enzymes, including arginase and alkaline phosphatase in the liver. (From AMA Drug Evaluations Annual 1992, p2035)
Any of the processes by which nuclear, cytoplasmic, or intercellular factors influence the differential control of gene action in fungi.
A chemical reaction in which an electron is transferred from one molecule to another. The electron-donating molecule is the reducing agent or reductant; the electron-accepting molecule is the oxidizing agent or oxidant. Reducing and oxidizing agents function as conjugate reductant-oxidant pairs or redox pairs (Lehninger, Principles of Biochemistry, 1982, p471).
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A flavoring agent. It is the intermediate product in the two-step bioconversion of ferulic acid to vanillin. (J Biotechnol 1996;50(2-3):107-13).
An organochlorine insecticide that is carcinogenic.
A man-made compound previously used to control termites and other insects. Even though production of heptachlor was phased out of use in the United States during the late 1980's it remains in soil and hazardous waste sites. It is clearly toxic to animals and humans but, the International Agency for Research on Cancer (IARC) has determined that heptachlor is not classifiable as to its carcinogenicity to humans. (From ATSDR Public Heath Statement, April 1989)
A copper-containing oxidoreductase enzyme that catalyzes the oxidation of 4-benzenediol to 4-benzosemiquinone. It also has activity towards a variety of O-quinols and P-quinols. It primarily found in FUNGI and is involved in LIGNIN degradation, pigment biosynthesis and detoxification of lignin-derived products.
A subclass of enzymes which includes all dehydrogenases acting on primary and secondary alcohols as well as hemiacetals. They are further classified according to the acceptor which can be NAD+ or NADP+ (subclass 1.1.1), cytochrome (1.1.2), oxygen (1.1.3), quinone (1.1.5), or another acceptor (1.1.99).
A large and heterogenous group of fungi whose common characteristic is the absence of a sexual state. Many of the pathogenic fungi in humans belong to this group.
The body of a fungus which is made up of HYPHAE.
Compounds consisting of two or more fused ring structures.
The rotation of linearly polarized light as it passes through various media.
A group of compounds that are derivatives of methoxybenzene and contain the general formula R-C7H7O.
A white crystalline compound prepared by condensation of benzaldehyde in potassium cyanide and used in organic syntheses. This should not be confused with benzoin gum from STYRAX.
Ribonucleic acid in fungi having regulatory and catalytic roles as well as involvement in protein synthesis.
Any liquid or solid preparation made specifically for the growth, storage, or transport of microorganisms or other types of cells. The variety of media that exist allow for the culturing of specific microorganisms and cell types, such as differential media, selective media, test media, and defined media. Solid media consist of liquid media that have been solidified with an agent such as AGAR or GELATIN.
A characteristic feature of enzyme activity in relation to the kind of substrate on which the enzyme or catalytic molecule reacts.
A cellulose derivative which is a beta-(1,4)-D-glucopyranose polymer. It is used as a bulk laxative and as an emulsifier and thickener in cosmetics and pharmaceuticals and as a stabilizer for reagents.
The study of the structure, growth, function, genetics, and reproduction of fungi, and MYCOSES.
Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.
Native, inorganic or fossilized organic substances having a definite chemical composition and formed by inorganic reactions. They may occur as individual crystals or may be disseminated in some other mineral or rock. (Grant & Hackh's Chemical Dictionary, 5th ed; McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Worthless, damaged, defective, superfluous or effluent material from industrial operations.
A genus of basidiomycetous fungi, family POLYPORACEAE, order POLYPORALES, that grows on logs or tree stumps in shelflike layers. The species P. ostreatus, the oyster mushroom, is a choice edible species and is the most frequently encountered member of the genus in eastern North America. (Alexopoulos et al., Introductory Mycology, 4th ed, p531)
The normality of a solution with respect to HYDROGEN ions; H+. It is related to acidity measurements in most cases by pH = log 1/2[1/(H+)], where (H+) is the hydrogen ion concentration in gram equivalents per liter of solution. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Yeast-like ascomycetous fungi of the family Saccharomycetaceae, order SACCHAROMYCETALES isolated from exuded tree sap.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
The rate dynamics in chemical or physical systems.
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
The visually perceived property of objects created by absorption or reflection of specific wavelengths of light.
Reproductive bodies produced by fungi.
Substances or energies, for example heat or light, which when introduced into the air, water, or land threaten life or health of individuals or ECOSYSTEMS.
A strong oxidizing agent used in aqueous solution as a ripening agent, bleach, and topical anti-infective. It is relatively unstable and solutions deteriorate over time unless stabilized by the addition of acetanilide or similar organic materials.
An exocellulase with specificity for a variety of beta-D-glycoside substrates. It catalyzes the hydrolysis of terminal non-reducing residues in beta-D-glucosides with release of GLUCOSE.
The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.
The presence of bacteria, viruses, and fungi in the soil. This term is not restricted to pathogenic organisms.
Chemicals and substances that impart color including soluble dyes and insoluble pigments. They are used in INKS; PAINTS; and as INDICATORS AND REAGENTS.
A condensation product of riboflavin and adenosine diphosphate. The coenzyme of various aerobic dehydrogenases, e.g., D-amino acid oxidase and L-amino acid oxidase. (Lehninger, Principles of Biochemistry, 1982, p972)
An NAD+ dependent enzyme that catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate to 3-carboxy-4-methyl-2-oxopentanoate. It is involved in the biosynthesis of VALINE; LEUCINE; and ISOLEUCINE.
The class of all enzymes catalyzing oxidoreduction reactions. The substrate that is oxidized is regarded as a hydrogen donor. The systematic name is based on donor:acceptor oxidoreductase. The recommended name will be dehydrogenase, wherever this is possible; as an alternative, reductase can be used. Oxidase is only used in cases where O2 is the acceptor. (Enzyme Nomenclature, 1992, p9)
Single-stranded complementary DNA synthesized from an RNA template by the action of RNA-dependent DNA polymerase. cDNA (i.e., complementary DNA, not circular DNA, not C-DNA) is used in a variety of molecular cloning experiments as well as serving as a specific hybridization probe.
Structurally related forms of an enzyme. Each isoenzyme has the same mechanism and classification, but differs in its chemical, physical, or immunological characteristics.
The color-furnishing portion of hemoglobin. It is found free in tissues and as the prosthetic group in many hemeproteins.
The measurement of the amplitude of the components of a complex waveform throughout the frequency range of the waveform. (McGraw-Hill Dictionary of Scientific and Technical Terms, 6th ed)
Highly reactive molecules with an unsatisfied electron valence pair. Free radicals are produced in both normal and pathological processes. They are proven or suspected agents of tissue damage in a wide variety of circumstances including radiation, damage from environment chemicals, and aging. Natural and pharmacological prevention of free radical damage is being actively investigated.
A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.
The complete gene complement contained in a set of chromosomes in a fungus.
An analytical method used in determining the identity of a chemical based on its mass using mass analyzers/mass spectrometers.

TMC-69, a new antitumor antibiotic with Cdc25A inhibitory activity, produced by Chrysosporium sp. TC1068. Taxonomy, fermentation and biological activities. (1/24)

A new antibiotic designated TMC-69 has been isolated from the fermentation broth of a fungal strain Chrysosporium sp. TC 1068. TMC-69 exhibited moderate in vitro cytotoxic activity. TMC-69-6H, a derivative of TMC-69 prepared by hydrogenation, possessed more potent in vitro cytotoxicity than TMC-69, and exhibited in vivo antitumor activity against murine P388 leukemia and B16 melanoma. TMC-69-6H was found to specifically inhibit Cdc25A and B phosphatases.  (+info)

Pulmonary colonization by Chrysosporium zonatum associated with allergic inflammation in an immunocompetent subject. (2/24)

We report a case of noninvasive pulmonary disease due to Chrysosporium zonatum in an immunocompetent male. The fungus colonized an existing tuberculous cavity and was isolated from transbronchial lavage fluid and from a percutaneous aspiration specimen. The disease was accompanied by the unusual feature of an allergic reaction. The fungus ball was successfully treated by intracavitary administration of amphotericin B. C. zonatum is the anamorph of the heterothallic ascomycete Uncinocarpus orissi, and the identity of the case isolate was verified by formation of ascospores in mating tests with reference isolates.  (+info)

Non-sporulating Chrysosporium: an opportunistic fungal infection in a neutropenic patient. (3/24)

We report a case of an invasive infection with non-sporulating Chrysosporium species in a patient who was treated with chemotherapy for relapsed acute lymphoblastic leukemia. This patient presented with a persistent lobar pneumonia, skin lesions, and possible involvement of the central nervous system. The patient responded to treatment with amphotericin B and oral itraconazole.  (+info)

False identification of Coccidioides immitis: do molecular methods always get it right? (4/24)

rRNA sequence analysis of a partial region of the 18S and 5.8S-internal transcribed spacer 2 (ITS2) region of Chrysosporium keratinophilum highlights its potential molecular misidentification as Coccidioides immitis. Molecular identification of medically important fungi should not be based solely on sequence analysis of the 18S rRNA gene but should be confirmed by sequence analysis of an additional rRNA gene locus, such as the ITS region(s).  (+info)

Specific xyloglucanases as a new class of polysaccharide-degrading enzymes. (5/24)

Three specific xyloglucanases (XGs) were isolated from Aspergillus japonicus (32 kDa, pI 2.8), Chrysosporium lucknowense (78 kDa, pI 3.8) and Trichoderma reesei (75-105 kDa, pI 4.1-4.3). The characteristic feature of these enzymes was their high specific activity toward tamarind xyloglucan, whereas the activity against carboxymethylcellulose (CMC) and barley beta-glucan was absent or very low. Peptide mass fingerprinting using MALDI-TOF mass spectrometry showed that the T. reesei XG represents Cel74A, whose gene has been discovered recently (GenBank accession no. AY281371 ), but the enzyme has not been characterized and described elsewhere. Tryptic peptides from A. japonicus and C. lucknowense xyloglucanases did not show any identity to those from known glycoside hydrolases. All enzymes produced XXXG, XXLG/XLXG and XLLG oligosaccharides as the end products of xyloglucan hydrolysis. A. japonicus XG displayed an endo-type of attack on the polymeric substrate, while the mode of action of two other xyloglucanases was similar to the exo-type, when oligosaccharides containing four glucose residues in the main chain were split off the ends of xyloglucan molecules. These results together with growing literature data allow concluding that specific xyloglucanases may represent a new class of glycoside hydrolases, which are different from regular endo-1,4-beta-glucanases.  (+info)

False-positive Histoplasma capsulatum Gen-Probe chemiluminescent test result caused by a Chrysosporium species. (6/24)

We describe a case in which the Histoplasma capsulatum AccuProbe test displayed cross-reactivity with a respiratory isolate thought to be Histoplasma but not morphologically consistent with H. capsulatum. The isolate was later identified as the Chrysosporium anamorph of Nannizziopsis vriesii by sequence analysis and phenotypic data.  (+info)

Overproduction of lignin-degrading enzymes by an isolate of Phanerochaete chrysosporium. (7/24)

Phanerochaete chrysosporium is a white rot fungus which secretes a family of lignin-degrading enzymes under nutrient limitation. PSBL-1 is a mutant of this organism that generates the ligninolytic system under nonlimiting conditions during primary metabolism. Lignin peroxidase, manganese peroxidase, and glyoxal oxidase activities for PSBL-1 under nonlimiting conditions were 4- to 10-fold higher than those of the wild type (WT) under nitrogen-limiting conditions. PSBL-1 was still in the log phase of growth while secreting the enzymes, whereas the WT had ceased to grow by this time. As in the WT, manganese(II) increased manganese peroxidase activity in the mutant. However, manganese also caused an increase in lignin peroxidase and glyoxal oxidase activities in PSBL-1. Addition of veratryl alcohol to the culture medium stimulated lignin peroxidase activity, inhibited glyoxal oxidase activity, and had little effect on manganese peroxidase activity in PSBL-1, as in the WT. Fast protein liquid chromatography (FPLC) analysis shows production of larger amounts of isozyme H2 in PSBL-1 than in the WT. These properties make PSBL-1 very useful for isolation of large amounts of all ligninolytic enzymes for biochemical study, and they open the possibility of scale-up production for pratical use.  (+info)

Pannorin, a new 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor produced by Chrysosporium pannorum. (8/24)

Pannorin, a naphthopyrone that inhibits 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase, the rate-limiting enzyme in cholesterol synthesis, was isolated from a culture broth of Chrysosporium pannorum M10539 by solvent extraction, Bio-Gel P-6 column chromatography and reverse phase HPLC (Silica ODS). Spectroscopic analyses of the compound yielded 4,8,10-trihydroxy-5-methyl-2H-naphtho[1,2-b]pyran-2-one as the proposed structure. Pannorin inhibited HMG-CoA reductase and in vitro sterol synthesis 50% at a concentration of 160 microM.  (+info)

A case of disseminated bilateral pulmonary adiaspiromycosis is reported in a two year old Finnish girl. She recovered from this rare infection after treatment with amphotericin B. She is the first human case of adiaspiromycosis in Scandinavia and she is the youngest child with this disease reported so far. Electron microscopy showed that the three layers of the spore wall were not typical; rather, there seemed to be a gradual transition between the main wall zones, which may be split into an indefinite number of thin layers. Varying numbers and thicknesses were seen with different staining methods, and in different spores. Diagnosis relies on recognition of the fungus in a pulmonary biopsy specimen, because there are no reliable serological tests and culture of the fungus is time consuming and not always successful. It was thought that this patient had become infected as a result of contact with soil dust containing the spores in the yard surrounding her home, and as a result of her mothers ...
Xyloglucanases - Instruments Consumables Reagents Advanced BioMatrix,RANDOX,RANDOX ELISA,Biomedical, biochemical reagents, laboratory supplies, equipment, antibodies, ELISA kits, diagnostic reagents, methods of experimental techniques, general analytical instruments, material testing instruments and equipment, used laboratory equipment, instruments and equipment, life sciences, environmental monitoring equipment , measurement, measuring instruments, rotating wall bioreactor, three-dimensional tissue / stem cell culture system; microcapsule
Come help us celebrate World Snake Day! Join us at the Nature Center as we have a discussion about reptiles and meet our very own black rat snake. Fun for all ages! (30 min.)
Successful treatment of Chrysosporium keratitis with voriconazole Onsiri Thanathanee, Chavakij Bhoomibunchoo, Orapin Anutarapongpan, Olan Suwan-apichon, Yosanan Yospaiboon KKU Eye Center, Department of Ophthalmology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand Objective: To report a patient with severe Chrysosporium keratitis successfully treated by voriconazole.Method: Case report.Results: A 37-year-old healthy male presented with irritation, pain and reduced vision in his left eye after mud contamination. Examination demonstrated corneal stromal infiltration, endothelial plaque and hypopyon. Corneal scrapings demonstrated numerous septate hyphae, and specimen cultures were positive for Chrysosporium sp. The lesion did not respond to aggressive topical 5% natamycin, 0.15% topical amphotericin B and oral itraconazole. The patient was then treated by topical 1% voriconazole every hour. Intracameral and intrastromal voriconazole injections (50 μg/0.1 mL) were also undertaken. The
Bartlett, R D. and Bartlett, P.P. 1999. A Field Guide to Texas Reptiles and Amphibians. Gulf Publishing Company, Houston, Texas. xviii + 331 pp.. Burbrink, F.T. 2001. Systematics of the eastern ratsnake complex (Elaphe obsoleta). Herpetological Monographs 15: 1-53.. Burbrink, F.T. and Lawson, R. 2007. How and when did Old World rat snakes disperse into the New World? Molecular Phylogenetics and Evolution 43: 173-189.. Burbrink, F.T., Lawson, R. and Slowinski, J.B. 2000. Mitochondrial DNA phylogeography of the North American rat snake (Elaphe obsoleta): a critique of the subspecies concept. Evolution 54: 2107-2114.. Conant, R. and Collins, J.T. 1991. A Field Guide to Reptiles and Amphibians: Eastern and Central North America. Third Edition. Houghton Mifflin Company, Boston, Massachusetts.. Crother, B.I., Boundy, J., Campbell, J.A., de Quieroz, K., Frost, D., Green, D.M., Highton, R., Iverson, J.B., McDiarmid, R.W., Meylan, P.A., Reeder, T.W., Seidel, M.E., Sites Jr, J.W., Tilley, S.G. and Wake, ...
N. pustulatus has been an enigmatic species of burying beetle because it was never found breeding on a small carcass. Drs. Gabriel Blouin-Demers and Patrick Weatherhead (University of Illinois-Urbana) found N. pustulatus adults and larvae in the nests of the black rat snake (Elaphe obsoleta). Working in our lab, Garrison Smith (M.S., University of Arizona) determined that this species is well adapted to exploiting reptile eggs using many of the same behaviors that congeners use when exploiting carcasses (see September, 2009 issue of Natural History). The closest phylogenetic relatives of N. pustulatus (determined by Derek Sikes, University of Alaska-Fairbanks Museum) have not been studied so it is unknown whether additional nicrophorine species act as parasitoids of reptiles. ...
N. pustulatus has been an enigmatic species of burying beetle because it was never found breeding on a small carcass. Drs. Gabriel Blouin-Demers and Patrick Weatherhead (University of Illinois-Urbana) found N. pustulatus adults and larvae in the nests of the black rat snake (Elaphe obsoleta). Working in our lab, Garrison Smith (M.S., University of Arizona) determined that this species is well adapted to exploiting reptile eggs using many of the same behaviors that congeners use when exploiting carcasses (see September, 2009 issue of Natural History). The closest phylogenetic relatives of N. pustulatus (determined by Derek Sikes, University of Alaska-Fairbanks Museum) have not been studied so it is unknown whether additional nicrophorine species act as parasitoids of reptiles. ...
Gluconic acid is a multifunctional organic acid which has increasing interest in different application because of its non-corrosiveness, non-toxicity, readily biodegradability and environmentally friendly properties ...
A marked enhancement in the production of a highly alkaline and thermostable pectinase by Bacillus pumilus dcsr1 in submerged fermentation by using statistical
Synonyms for rat snake in Free Thesaurus. Antonyms for rat snake. 13 words related to rat snake: colubrid, colubrid snake, corn snake, Elaphe guttata, red rat snake, black rat snake, blacksnake, Elaphe obsoleta.... What are synonyms for rat snake?
The research on the growth of Phanerochaete chrysosporium in the form of pellet was made in this paper to examine the effects of physical and chemical conditions on the formation of mycelial pellets.The experimental results showed that the microorganism pellets of smooth surface,even size(diameter)and good mechanical properties could be obtained at the follwoing culture conditions:pH4 5,10 6 spores/ml in inoculum,0 1% Tween 80 in medium,high C/N and on the shaker of 150r/min for 3d.Using P.chrysosporium mycelial pellets in the biosorption of Pb 2+ from aqueous solution,the uptake reached the maximum when the diameter of pellet was in the range of 1 5-1 7mm.When the pellets were pretreated by boiling in 0 2mol/L NaOH for 40min,the Pb 2+ uptake was far higher than the pellets without pretreatment,and the removal efficiency could reach above 95%.The research results showed that the removal of Pb 2+ from aqueous solution by P.chrysosporium mycelial pellets was practical.
Screening and Optimization of Submerged Fermentation of Lipolytic Aspergillus oryzae. Asma Ahmed,a,* Rehana Badar,a and Noman Khalique b. Lipases are enzymes commonly used in industry. This study describes the production of lipolytic enzyme via a newly isolated strain of Aspergillus oryzae under a submerged fermentation process. Five strains of A. oryzae were isolated from oil-contaminated soil and water as well as dead decaying organic matter. Qualitative screening revealed that A. oryzae RBM4 strain was a lipase producer, and the process was optimized for enhanced production. Incubation time, incubation temperature, initial pH, use of agricultural by-products, nitrogen sources, and substrates were tested. The results revealed that initial pH 5.5(12.7 U/mL/min) in 72 h (19.39 U/mL/min) at 30 °C (27.40 U/mL/min) sorghum (35.66 U/mL/min), NaNO3 (17% more than blank), yeast extract (47.95 U/mL/min), and Shan ghee (58.12 U/mL/min) were the best conditions. Extracellular lipase production was ...
Proteome IDi ,p>The proteome identifier (UPID) is the unique identifier assigned to the set of proteins that constitute the ,a href=http://www.uniprot.org/manual/proteomes_manual>proteome,/a>. It consists of the characters UP followed by 9 digits, is stable across releases and can therefore be used to cite a UniProt proteome.,p>,a href=/help/proteome_id target=_top>More...,/a>,/p> ...
Isolation and purification of Pyranose 2-oxidase from Phanerochaete chrysosporium and characterization of gene structure and regulation
Domain: Eukaryota • Regnum: Fungi • Phylum: Ascomycota • Subphylum: Pezizomycotina • Classis: Eurotiomycetes • Subclassis: Eurotiomycetidae • Ordo: Onygenales • Familia: Onygenaceae • Genus: Coccidioides G.W. Stiles 1896 ...
ID C5GNE1_AJEDR Unreviewed; 606 AA. AC C5GNE1; DT 28-JUL-2009, integrated into UniProtKB/TrEMBL. DT 07-SEP-2016, sequence version 2. DT 20-DEC-2017, entry version 48. DE SubName: Full=Actin polymerization protein Bzz1 {ECO:0000313,EMBL:EEQ91176.2}; GN ORFNames=BDCG_06296 {ECO:0000313,EMBL:EEQ91176.2}; OS Ajellomyces dermatitidis (strain ER-3 / ATCC MYA-2586) (Blastomyces OS dermatitidis). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Onygenales; Ajellomycetaceae; Blastomyces. OX NCBI_TaxID=559297 {ECO:0000313,EMBL:EEQ91176.2, ECO:0000313,Proteomes:UP000002039}; RN [1] {ECO:0000313,Proteomes:UP000002039} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ER-3 / ATCC MYA-2586 {ECO:0000313,Proteomes:UP000002039}; RX PubMed=26439490; DOI=10.1371/journal.pgen.1005493; RA Munoz J.F., Gauthier G.M., Desjardins C.A., Gallo J.E., Holder J., RA Sullivan T.D., Marty A.J., Carmen J.C., Chen Z., Ding L., Gujja S., RA Magrini V., Misas E., Mitreva M., ...
The plant cell wall is a complex material in which the cellulose microfibrils are embedded within a mesh of other polysaccharides, some of which are loosely termed hemicellulose. One such hemicellulose is xyloglucan, which displays a β-1,4-linked D-glucose backbone substituted with xylose, galactose, and occasionally fucose moieties. Both xyloglucan and the enzymes responsible for its modification and degradation are finding increasing prominence, reflecting both the drive for enzymatic biomass conversion, their role in detergent applications, and the utility of modified xyloglucans for cellulose fiber modification. Here we present the enzymatic characterization and three-dimensional structures in ligand-free and xyloglucan-oligosaccharide complexed forms of two distinct xyloglucanases from glycoside hydrolase families GH5 and GH12. The enzymes, Paenibacillus pabuli XG5 and Bacillus licheniformis XG12, both display open active center grooves grafted upon their respective (β/α)8 and β-jelly ...
Utiger et al. (2002) examined mtDNA variation in New World and Old World Elaphe and determined that North American rat snakes included in the genus Elaphe formed a monophyletic limeage that is distinct from Old World snakes that also have been regarded as Elaphe. They resurrected the genus Pantherophis for the rat snakes north of Mexico, including the following species: Pantherophis obsoletus (and P. alleghaniensis and P. spiloides, if one recognizes those taxa as species), P. guttatus, P. emoryi, P. vulpinus, P. gloydi, and P. bairdi. Crother et al. (2003) did not follow this taxonomy pending further research, and retained the traditional concept of Elaphe. Burbrink and Lawson (2007) agreed with Utiger et al. (2002) that these species do not belong in Elaphe, but suggested that the genus Pantherophis might belong in Pituophis. We place this species in Pantherophis, following Utiger et al. (2002) pending further information on the relationships of these taxa ...
Although they prefer rodents, rat snakes will bite humans if cornered. Avoid infection wounds by knowing how to respond to its aggressions knowing its natural history.
Pantherophis alleghaniensis, the Eastern rat snake; Lowndes county, Georgia (28 April 2012). Note that some consider Gray-patterned Eastern rat snakes in this area to be Pantherophis spiloides, the Gray rat snake. Learn more about this complex at iNaturalist.org.
The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP) from aqueous solution was investigated. The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP degradation was examined. Results showed that Lip and Mnp were not essential to 2,4-DCP degradation while their presence enhanced the degradation process and reaction rate. In sequential batch experiment, the bioactivity of immobilized cells was recovered and improved during the culture and the maximum degradation rate constant of 13.95 mg (Ld)−1 could be reached. In continuous bioreactor test, the kinetic behavior of the Phanerochaete chrysosporium immobilized on loofa sponge was found to follow the Monod equation. The maximum reaction rate was 7.002 mg (Lh)−1, and the saturation constant was 26.045 mg L−1. ...
The first steps of wood degradation by fungi lead to the release of toxic compounds known as extractives. To better understand how lignolytic fungi cope with the toxicity of these molecules, a transcriptomic analysis of Phanerochaete chrysosporium genes was performed in presence of oak acetonic extracts. It reveals that in complement to the extracellular machinery of degradation, intracellular antioxidant and detoxification systems contribute to the lignolytic capabilities of fungi presumably by preventing cellular damages and maintaining fungal health. Focusing on these systems, a glutathione transferase (PcGTT2.1) has been selected for functional characterization. This enzyme, not characterized so far in basidiomycetes, has been first classified as a GTT2 in comparison to the Saccharomyces cerevisiae isoform. However, a deeper analysis shows that GTT2.1 isoform has functionally evolved to reduce lipid peroxidation by recognizing high-molecular weight peroxides as substrates. Moreover, the ...
Abstract: The central nervous system (CNS) of a metamorphically competent larva of the caenogastropod Ilyanassa obsoleta contains a medial, unpaired apical ganglion (AG) of approximately 25 neurons that lies above the commissure connecting the paired cerebral ganglia. The AG, also known as the cephalic or apical sensory organ (ASO), contains numerous sensory neurons and innervates the ciliated velar lobes, the larval swimming and feeding structures. Before metamorphosis, the AG contains 5 serotonergic neurons and exogenous serotonin can induce metamorphosis in competent larvae. The AG appears to be a purely larval structure as it disappears within 3 days of metamorphic induction. In competent larvae, most neurons of the AG display nitric oxide synthase (NOS)-like immunoreactivity and inhibition of NOS activity can induce larval metamorphose. Because nitric oxide (NO) can prevent cells from undergoing apoptosis, a form of programmed cell death (PCD), we hypothesize that inhibition of NOS activity ...
Lawson, B., Franklinos, L.H.V., Rodriguez-Ramos Fernandez, J., Wend-Hansen, C., Nair, S., Macgregor, S., John, S., Pizzi, R., Núñez, A., Ashton, P., Cunningham, A.A., de Pinna, E. (n.d.). Salmonella Enteritidis ST183: emerging and endemic biotypes affecting western European hedgehogs (Erinaceus europaeus) and people in Great Britain. Scientific Reports, In Press.. Franklinos, L. H. V., Lorch, J. M., Bohuski, E., Rodriguez-Ramos Fernandez, J., Wright, O. N., Fitzpatrick, L., … Lawson, B. (2017). Emerging fungal pathogen Ophidiomyces ophiodiicola in wild European snakes. Scientific Reports, 7, 3844. https://doi.org/10.1038/s41598-017-03352-1. Franklinos, L. H. V., Masters, N., Feltrer, Y., Pocknell, A., Bolt, D. M., Dakin, S., Berry, K., Molenaar, F.M. (2017). The management of an oral anaplastic sarcoma in a pygmy hippopotamus (Choeropsis liberiensis) using intralesional chemotherapy. Journal of Zoo and Wildlife Medicine, 48(1), 260-264. https://doi.org/10.1638/2016-0015.1. Franklinos, L.H.V. ...
ID C1GVY4_PARBA Unreviewed; 857 AA. AC C1GVY4; DT 26-MAY-2009, integrated into UniProtKB/TrEMBL. DT 04-FEB-2015, sequence version 2. DT 07-JUN-2017, entry version 36. DE RecName: Full=V-type proton ATPase subunit a {ECO:0000256,RuleBase:RU361189}; GN ORFNames=PAAG_02679 {ECO:0000313,EMBL:EEH40703.2}; OS Paracoccidioides lutzii (strain ATCC MYA-826 / Pb01) (Paracoccidioides OS brasiliensis). OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; OC Eurotiomycetidae; Onygenales; Onygenales incertae sedis; OC Paracoccidioides. OX NCBI_TaxID=502779 {ECO:0000313,EMBL:EEH40703.2, ECO:0000313,Proteomes:UP000002059}; RN [1] {ECO:0000313,EMBL:EEH40703.2, ECO:0000313,Proteomes:UP000002059} RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=ATCC MYA-826 / Pb01 {ECO:0000313,Proteomes:UP000002059}; RX PubMed=22046142; DOI=10.1371/journal.pgen.1002345; RA Desjardins C.A., Champion M.D., Holder J.W., Muszewska A., RA Goldberg J., Bailao A.M., Brigido M.M., Ferreira M.E., Garcia ...
The glycogen-synthase-kinase 3 (GSK-3) is an important target in drug discovery. This enzyme is involved in the signaling pathways of type 2 diabetes, neurological disorders, cancer, and other diseases. Therefore, inhibitors of GSK-3 are promising drug candidates for the treatment of a broad range of diseases. Here we report pannorin (1), alternariol (2), and alternariol-9-methylether (3) to be promising inhibitors of the isoform GSK-3β showing sub-μM IC50 values. The in vitro inhibition is in the range of the known highly active GSK-3β inhibitor TDZD-8. Compounds 1-3 have a highly oxygenated benzocoumarin core structure in common, which suggests that this may be a new structural feature for efficient GSK-3β inhibition.
Conclusions: The GGSF and GANM substrates favored an antimicrobial activity against Staphylococcus aureus during the second and third hours of fermentation (inhibition diameter was 6.11% and 4.72%, respectively). The GGSF, GANM and CS substrates did not present statistically significant differences for the production of antimicrobial substances against Staphylococcus aureus. Therefore, GGSF and GANM can be considered as viable and economical alternative nitrogen sources for the production of the antimicrobial compounds formed by Weissella confusa in submerged fermentations ...
TABLE-US-00002 Program: CLUSTALW, Default parameters: Protein Gap Open Penalty 10.0 Protein Gap Extension Penalty 0.2 Protein weight matrix: Gonnet series GI No. of the reference S_ID DB SEQ ID NO Organism sequences Aa Position s1 seq_ID 2 Zymomonas mobilis AAV90172.1 F 486 s20 seq_ID 3 Streptomyces coelicolor CAB39697.1 F 449 s911 seq_ID 4 Acetobacter pasteurianus BAH99456.1 F 481 s2 seq_ID 5 Bradyrhizobium sp. ABQ33590.1 F 447 s940 seq_ID 6 Zymomonas mobilis EER62728.1 F 438 s949 seq_ID 7 Acidithiobacillus caldus EET25937.1 Y 432 s167 seq_ID 8 Acidithiobacillus ferrooxidans ACH84004.1 Y 429 s41 seq_ID 9 Acidobacterium capsulatum ACO34244.1 F 458 s36 seq_ID 10 Acidothermus cellulolyticus ABK53469.1 F 426 s83 seq_ID 11 Adiantum capillus-veneris BAF93209.1 Y 436 s143 seq_ID 12 Ajellomyces capsulatus EDN09769.1 F 496 s995 seq_ID 13 Ajellomyces capsulatus EER40510.1 -- 432 s163 seq_ID 14 Ajellomyces capsulatus EEH02950.1 F 429 s13 seq_ID 15 Alicyclobacillus acidocaldarius EED08231.1 Y 420 s14 ...
TY - JOUR. T1 - Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium. T2 - Activity in the absence of added hydrogen peroxide. AU - Kuan, I. Ching. AU - Tien, Ming. PY - 1993. Y1 - 1993. N2 - Mn peroxidases are H2O2-utilizing hemeproteins secreted by the lignin-degrading fungus Phanerochaete chrysosporium. We show here that glyoxylate is capable of supporting Mn peroxidase activity without added H2O2. This glyoxylate-supported activity is dependent upon Mn2+ and dioxygen. The involvement of superoxide is demonstrated by inhibition by superoxide scavenging agents, superoxide dismutase, or tetranitromethane. The addition of catalase resulted in dioxygen evolution, indicating that H2O2 is an intermediate in the reaction. Formate is one of the oxidation products of glyoxylate as detected by the formate dehydrogenase assay. The generation of H2O2 in the presence of Mn2+ and Mn peroxidase results in Mn3+ formation. Consequently, we show that the direct reaction ...
The temporal and spatial effects of selenite (SeO32−) on the physical properties and respiratory activity of Phanerochaete chrysosporium biofilms, grown in flow-cell reactors, were investigated using oxygen microsensors and confocal laser scanning microscopy (CLSM) imaging. Exposure of the biofilm to a SeO32− load of 1.67 mg Se L−1 h−1 (10 mg Se L−1 influent concentration), for 24 h, resulted in a 20% reduction of the O2 flux, followed by a ∼10% decrease in the glucose consumption rate. Long-term exposure (4 days) to SeO32− influenced the architecture of the biofilm by creating a more compact and dense hyphal arrangement resulting in a decrease of biofilm thickness compared to fungal biofilms grown without SeO32−. To the best of our knowledge, this is the first time that the effect of SeO32− on the aerobic respiratory activity on fungal biofilms is described.
Abstract: The apical ganglion (AG) of larval caenogastropods, such as Ilyanassa obsoleta, houses a sensory organ, contains five serotonergic neurons, innervates the muscular and ciliary components of the velum, and sends neurites into a neuropil that lies atop the cerebral commissure. During metamorphosis, the AG is lost. This loss had been postulated to occur through some form of programmed cell death (PCD), but it is possible for cells within the AG to be respecified or to migrate into adjacent ganglia. Evidence from histological sections is supported by results from a terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, which indicate that cells of the AG degenerate by PCD. PCD occurs after metamorphic induction by serotonin or by inhibition of nitric oxide synthase (NOS) activity. Cellular degeneration and nuclear condensation and loss were observed within 12 h of metamorphic induction by NOS inhibition and occur before loss of the velar lobes, the ciliated tissue used ...
1GPI: Family 7 Cellobiohydrolases from Phanerochaete Chrysosporium: Crystal Structure of the Catalytic Module of Cel7D (Cbh58) at 1.32 Angstrom Resolution and Homology Models of the Isozymes.
1C8I: Direct binding of hydroxylamine to the heme iron of Arthromyces ramosus peroxidase. Substrate analogue that inhibits compound I formation in a competetive manner.
The production of methionine by submerged fermentation using a mutant strain of Corynebacterium lilium was studied to determine suitable conditions for obtaining high productivity. The mutant strain resistant to the ...
Phanerochaete chrysosporium ATCC 24725 internal transcribed spacer 1, partial sequence; 5.8S ribosomal RNA gene and internal transcribed spacer 2, complete sequence; and 28S ribosomal RNA gene, partial ...
What makes it the worst, though, is that this wording gives the impression that evolution is a directed process. If a species needs some particular trait, somehow, natural selection will work toward acquiring that trait. Thats absurd! Yet time and time again, this author gives the impression that natural selection will do just that. For example, while discussing the Rat Snake, the author said, As a result, rat snakes have had to adapt to their local environments in an effort to avoid detection and hunt more effectively. In the case of nylon-eating bacteria, the author said, This is a very simple example of natural selection, where the most basic forms of life can adapt to whatever food the environment offers. Isnt that a hoot? According to this logic, if only birdseed is available in some area, then the crocodiles there can adapt to eat birdseed! ...
El proceso de transferir información de un emisor a un receptor a través de un canal determinado se llama comunicación. Entidades biológicas se han basado desde tiempos inmemoriales en mensajeros químicos para transmitir información; Válido para los organismos multicelulares, así como para las poblaciones de organismos unicelulares. Al ser compuestos químicos, estos mensajeros se ven limitados al alcanze de dicho sistema, por ejemplo: Aun mensajeros de largo alzanze, como las hormonas, estan ligadas al sistema quimico (cuerpo humano). En este proyecto, nuestra meta es hacer que la barrera química de la comunicación celular se vuelva obsoleta.Esto se traduce al uso de mensajeros no químicos, en este caso, los fotones. Nuestro canal entonces es la luz hecha apartir de paquetes de fotones o cuantas de energía, que transportaran información de los remitentes a los receptores, cruzando asi murallas quimicas. En consecuencia, nuestro sistema de comunicación ya no es contenida dentro de ...
El proceso de transferir información de un emisor a un receptor a través de un canal determinado se llama comunicación. Entidades biológicas se han basado desde tiempos inmemoriales en mensajeros químicos para transmitir información; Válido para los organismos multicelulares, así como para las poblaciones de organismos unicelulares. Al ser compuestos químicos, estos mensajeros se ven limitados al alcanze de dicho sistema, por ejemplo: Aun mensajeros de largo alzanze, como las hormonas, estan ligadas al sistema quimico (cuerpo humano). En este proyecto, nuestra meta es hacer que la barrera química de la comunicación celular se vuelva obsoleta.Esto se traduce al uso de mensajeros no químicos, en este caso, los fotones. Nuestro canal entonces es la luz hecha apartir de paquetes de fotones o cuantas de energía, que transportaran información de los remitentes a los receptores, cruzando asi murallas quimicas. En consecuencia, nuestro sistema de comunicación ya no es contenida dentro de ...
The elaphe are the main genus of rat snakes. It contains different species. These snakes are non-venomous but their bite. Read more ...
GenDR A curated database of genes associated with dietary restriction in model organisms either from genetic manipulation experiments or gene expression profiling.. ...
Influence of Carbon Source on the Production of Extracellular Ligninolytic Enzymes by Phanerochaete chrysosporium. Fangfang Wang,a Mingqiang Ai,a Guihua Yang,b Jiachuan Chen,b Xiulan Chen,a and Feng Huang a,*. The effect of altering the carbon source in the growing environment was investigated relative to the production of ligninolytic enzymes by Phanerochaete chrysosporium. Glucose, cellobiose, and cellulose (or mixtures thereof) were used as the carbon sources. Glucose oxidase and glyoxal oxidase activities in all carbon sources were produced during cultivation. High peak levels (0.17 to 0.24 IU/mL) of manganese peroxidase activity were observed only in mediums containing oligosaccharides. Lignin peroxidase activity was high in glucose medium (0.21 IU/mL of peak value); however, minimal amounts were formed in the cellulose medium (0.01 IU/mL of peak value). High amounts of cellobiose:quinone oxidoreductase (3.33-3.99 IU/mL of peak value) and cellobiose dehydrogenase (0.04-0.2 IU/mL of peak ...
TY - JOUR. T1 - Oxidation of 1,2,4,5-tetramethoxybenzene by lignin peroxidase of Phanerochaete chrysosporium. AU - Koduri, Rao S.. AU - Whitwam, Ross E.. AU - Barr, David. AU - Aust, Steven D.. AU - Tien, Ming. PY - 1996/2/15. Y1 - 1996/2/15. N2 - We have reinvestigated the lignin peroxidase-catalyzed oxidation of 1,2,4,5-tetramethoxybenzene (TMB) by using presteady-state and steady-state kinetic methods. Our presteady-state kinetic results show that the reaction of compound I with TMB obeyed second order kinetics with a rate constant of 1.1 x 107 M-1 s-1. The reaction of compound II with TMB exhibits a hyperbolic concentration dependence with a K(d) of 16 μM and k = 24 s-1. The stoichiometry of TMB oxidation during steady state is two TMB cation radicals formed per H2O2 consumed. These results clearly show that TMB is a good substrate for both compounds I and II of lignin peroxidase.. AB - We have reinvestigated the lignin peroxidase-catalyzed oxidation of 1,2,4,5-tetramethoxybenzene (TMB) by ...
While most Ascomycetes tend to associate principally with plants, the dimorphic fungi Coccidioides immitis and Coccidioides posadasii are primary pathogens of immunocompetent mammals, including humans. Infection results from environmental exposure to Coccidiodies, which is believed to grow as a soil saprophyte in arid deserts. To investigate hypotheses about the life history and evolution of Coccidioides, the genomes of several Onygenales, including C. immitis and C. posadasii; a close, nonpathogenic relative, Uncinocarpus reesii; and a more diverged pathogenic fungus, Histoplasma capsulatum, were sequenced and compared with those of 13 more distantly related Ascomycetes. This analysis identified increases and decreases in gene family size associated with a host/substrate shift from plants to animals in the Onygenales. In addition, comparison among Onygenales genomes revealed evolutionary changes in Coccidioides that may underlie its infectious phenotype, the identification of which may ...
In spite of great variation in body size, habitat use, diet, and behavior, the lack of bold, readily apparent distinguishing marks can make identification of southeastern black snakes a daunting task for those inexperienced with snakes. Nonetheless, an informed observer can readily recognize the bright orange belly of the black swamp snake or the namesake ringed neck of the ring-necked snake, and may quickly learn to distinguish between the smooth, glossy sheen of the eastern indigo or black racer and the keeled, somewhat dull look of the black pine and black rat snakes. These snakes may seem nondescript at first glance, though knowledge of these and other more subtle, yet telltale characteristics will assist in the rewarding task of becoming familiar with the black snakes of the southeastern US.. Fortunately, there are a variety of books and websites that are extremely helpful references for use in determining the identity of an unknown non-venomous or venomous snake. In addition, these ...
Este trabalho teve por objetivo avaliar a degradação de deoxinivalenol por Aspergillus oryzae e Rhizopus oryzae durante fermentação submersa e correlacioná-la com a atividade de enzimas oxidoredutases. O meio submerso foi constituído por água destilada estéril contaminada com 50 μg de DON e inóculo de 4 × 106 esporos.mL-1 de meio das espécies fúngicas Aspergillus oryzae e Rhizopus oryzae, separadamente em cada experimento. A amostragem foi realizada a cada 24 horas de processo para medida da atividade específica da enzima peroxidase, e a cada 48 horas para a determinação dos níveis de DON. Os resultados mostraram que as espécies fúngicas avaliadas possuem capacidade de metabolizar DON, acompanhada de aumento na atividade da enzima oxidativa PO. No intervalo de 48 horas de fermentação, ocorreu a maior atividade específica da enzima peroxidase (ΔABS/minute.μg.protein-1), 800 e 198, correspondendo a maior velocidade de degradação de DON de 10.8 e 12.4 ppb/hour, ...
There also are copy-number changes within conserved fungal kinase families. Phylogenetic analysis of the MAPK cascade revealed Basidiomycota-specific duplications in the MAPK genes (S. cerevisiae FUS3/KSS1) which are involved in the pheromone response, whereas HOG1 a p38 kinase, is single copy throughout the sampled fungi (Fig. S3).. We examined two additional gene families of significant size (cytochrome P450 and hydrophobins) to ask if duplicated paralogs from these families also are found in restricted chromosome regions. The P450 gene family (125 genes contain the Pfam domain) includes genes with metabolic roles in monooxygenase metabolism, and the family is implicated in the degradation capabilities of Phanerochaete chrysosporium on substrates ranging from lignin to diesel fuel (26, 27). Phylogenetic analysis shows that the expansion of the family was independent in P. chrysosporium and C. cinerea (SI Text and Fig. S4). Although scattered members of the P450 and other expanded families are ...
INTRODUCTION. Lipases (triacylglycerol acylhydrolases, EC3.1.1.3) are water-soluble enzymes that catalyze the hydrolysis of ester bonds of insoluble triacylglycerols, releasing free fatty acids, mono-or diacylglycerolin into the oil-water interface (Treichel et al., 2010). These enzymes catalyze a wide range of reactions such as hydrolysis, esterification, trans-esterification, alcoholysis, acidolysis and aminolysis (Joseph; Ramteke; Thomas, 2008), and may be of animal, microbial or plant origin, with variations in catalytic properties, and can be obtained by solid-state fermentation or submerged fermentation (Annibale et al., 2006; Rigo et al., 2010; Papagora; Roukas; Kotzekidou, 2013).. Lipases are an important group of enzymes with various applications in the food, chemical and pharmaceutical industry, besides having great interest for the treatment of effluents with high lipid content (Jeganathan; Nakhla; Bassi, 2007; Ertugrul; Donnez; Takac, 2007). The use of lipases in effluents with high ...
Between March and May, snakes will begin to emerge from winters hibernation. After a few weeks, rat snakes begin to seek out a mate, typically in late April, May, and early June. Males tend to wait for the females to pass through their territory, and by using pheromones, will communicate and initiate the mating process with the female. A male will approach a female, line up with her and attempt to wrap his tail around her tail with their vents nearly touching. Some males will grasp females with their mouths to hold them in place and prevent their escape. The male will then erect his hemipene and insert it into the females cloaca while several small spines anchor the hemipene firmly. Mating can last only a few minutes or it can span the time of a few hours. Five weeks later, the female will lay around 5 to 27 eggs. The female will lay her eggs in a hidden area, under hollow logs or leaves, or in abandoned burrows. The eggs will hatch 55 to 60 days later. Like other reptiles, rat snakes have ...
Thailand has 200+ snake species with about 35 of them venomous and deadly (on land). I started this site to educate Thais, expats, and visitors to Thailand about snakes. Many people kill the snakes they see here, while in many cases - they are non-venomous and completely harmless. With this site I hope to give people a better idea of what is harmful and what isnt ...
Since it is open access you can read the paper for the full details. The main result is that it looks like separate and distinction lineages of R. rattus piggybacked on the expansion of humans. The main caveat, admitted in the article, is a reliance on mtDNA and the possibility of admixture and introgression across lineages explaining the current extant variance. The authors refer to paraphyly because it may be that all the descendants of modern black rats, as we understand them, may not be identified as black rats, probably due to their lack of adaptation and coexistence with humans.. Obviously well need to wait for autosomal studies which utilize many more markers. But lets grant the robustness of this finding: that the modern black rat lineages are a compound of a recent demographic expansion from a small population in western India, as well as long standing deep rooted populations across South and Southeast Asia, which independently entered into coexistence and parasitism with humans. I ...
This is a little Texas Rat Snake (more info here). Lots of people are quick to kill them and say they have no choice because these snakes are agressive.
Chrysosporium keratinophilum is regarded by some mycologists as the asexual state, or anamorph, however others argue that this ... The colonial appearance of A. fulvescens generally mimics that of species in the genus Trichophyton and Chrysosporium while ... "Destruction of hair by Chrysosporium keratinophilum". (April 1969). Transactions of the British Mycological Society 52(2):247- ...
Phanerochaete chrysosporium was grown on PVC in a mineral salt agar. Phanerochaete chrysosporium, Lentinus tigrinus, ...
Species of Geomyces have previously been placed in the genus Chrysosporium. This genus is characterized by short but distinct ...
Other bipolar species include the white rot fungus Phanerochaete chrysosporium and the edible mushroom Pholiota nameko. In the ... composed of homeodomain genes promotes nuclear migration and heterokaryosis in the white-rot fungus Phanerochaete chrysosporium ...
... is degraded by the organisms in sewage sludge as well as the fungus Phanaerocheate chrysosporium. Both wild and transgenic ...
Whereas, P. chrysosporium recorded decolorization to the maximum with the Nigrosin (90.15%) followed by Basic fuchsin (89.8%), ... Ligninolytic fungi, such as Phanerochaete chrysosporium, Bjerkandera adusta, and Pleurotus ostreatus, have the capacity of PAH ... nigrosin and basic fuchsin with Aspergillus niger and Phanerochaete chrysosporium and Congo red, a carcinogenic dye ...
Bacterial reduction of selenite protects P. chrysosporium against toxicity, while fungal degradation of phenols protects D. ... Co-cultures Delftia lacustris with Phanerochaete chrysosporium could be used for the bioremediation of petrochemical ... with simultaneous degradation of phenol by co-cultures of Phanerochaete chrysosporium and Delftia lacustris". Journal of ...
Examples of bioprospecting products used in bioremediation include Coriolopsis gallica- and Phanerochaete chrysosporium-derived ...
Phanerochaete chrysosporium (en). White rot fungus. DDT, BTEX, Dieldrin, Endodulfan, PCP. Phytostimulation. [7] ... Phanerochaete chrysosporium (en). White rot fungus. , BTEX, Dieldrin, Endodulfan, Pentachloronitro-benzène, PCP. ... Phanerochaete chrysosporium (en). White rot fungus. DDT, BTEX, Dieldrin, Endodulfan, Pentachloronitro-benzène. Phytostimulation ...
"Genome sequence of the lignocellulose degrading fungus Phanerochaete chrysosporium strain RP78". Nat. Biotechnol. 22 (6): 695- ...
The white rot fungus Phanerochaete chrysosporium can remove up to 80% of phenolic compounds from coking waste water.[25] ... "Biodegradation of phenolic compounds from coking wastewater by immobilized white rot fungus Phanerochaete chrysosporium". ...
"Degradation of lead-contaminated lignocellulosic waste by Phanerochaete chrysosporium and the reduction of lead toxicity". ...
Well-studied ligninolytic enzymes are found in Phanerochaete chrysosporium[31] and other white rot fungi. Some white rot fungi ... "Lignin-Degrading Enzyme from the Hymenomycete Phanerochaete chrysosporium Burds". Science. 221 (4611): 661-3. doi:10.1126/ ...
... previously known as Chrysosporium lucknowense C1". Industrial Biotechnology. 7 (3): 214-223. doi:10.1089/ind.2011.7.214.. ...
Of the 22 species, Cephalosporium acreomonium is the zygomycetous species of Umbelopsis, Chrysosporium sinense has very low ...
The fungus Aspergillus fumigatus effectively degrades plasticized PVC.[58] Phanerochaete chrysosporium has been grown on PVC in ... Phenol-formaldehyde, commonly known as bakelite, is degraded by the white rot fungus Phanerochaete chrysosporium.[69] ... Phanerochaete chrysosporium was grown on PVC in a mineral salt agar.[59] ... a mineral salt agar.[59] Phanerochaete chrysosporium, Lentinus tigrinus, Aspergillus niger, and Aspergillus sydowii can also ...
Enzim mangan peroksidase pada kulat Phanerochaete chrysosporium dikenal pasti sebagai salah satu enzim yang membenarkan ...
Corneal scrapings demonstrated numerous septate hyphae, and specimen cultures were positive for Chrysosporium sp. The lesion ... this is the first report on the use of voriconazole for Chrysosporium keratitis. Voriconazole may be an effective alternative ... To report a patient with severe Chrysosporium keratitis successfully treated by voriconazole.Method: Case report.Results: A 37- ... Successful treatment of Chrysosporium keratitis with voriconazole Onsiri Thanathanee, Chavakij Bhoomibunchoo, Orapin ...
Phanerochaete chrysosporium ATCC ® 24725™ Designation: VKM F-1767 [CBS 481.73, CCRC 36200, IMI 174727, NRRL 6361, QM 9998] ... Phanerochaete chrysosporium Burdsall (ATCC® 24725™) Alternate State: Sporotrichum pulverulentum Novobranova, Sporotrichum ... Stewart P, Gaskell J, Cullen D. A homokaryotic derivative of a Phanerochaete chrysosporium strain and its use in genomic ... Nucleotide (GenBank) : X51590 Phanerochaete chrysosporium GLG3 (LIP) gene for lignin peroxidase Nucleotide (GenBank) : X12698 ...
Understanding LiP promoters from Phanerochaete chrysosporium: A bioinformatic analysis. In: Xia, Xuhua, ed. Selected works in ... Influence of Populus Genotype on Gene Expression by the Wood Decay Fungus Phanerochaete chrysosporium ... Comparative transcriptome and secretome analysis of wood decay fungi Postia placenta and Phanerochaete chrysosporium ... Transcript patterns of Phanerochaete chrysosporium genes in organopollutant contaminated soils and in wood. ...
RPR113228, a novel farnesyl-protein transferase inhibitor produced by Chrysosporium lobatum.. Van der Pyl D, Cans P, Debernard ...
"Heterogeneity and regulation of manganese peroxidases from Phanerochaete chrysosporium.". Pease E.A., Tien M.. J. Bacteriol. ... sp,P78733,PEM3_PHACH Manganese peroxidase H3 OS=Phanerochaete chrysosporium OX=5306 PE=1 SV=2 ... Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum). ,p>This subsection of the ,a href="http://www.uniprot. ...
... chrysosporium and 82% (n = 8,258) of P. chrysosporium models aligned with C. subvermispora. Most (n = 5,443) of these pairs ... chrysosporium. Genes encoding manganese peroxidase numbered 13 and five in C. subvermispora and P. chrysosporium, respectively ... The P. chrysosporium and C. subvermispora genes were previously designated Pcfad2 and Csfad2 (29, 30), respectively. Transcript ... 1). In identical media, none of the P. chrysosporium MnP genes were up-regulated, but significant accumulation of two LiP gene ...
Phanerochaete chrysosporium (White-rot fungus) (Sporotrichum pruinosum)Imported. Automatic assertion inferred from database ... tr,Q12170,Q12170_PHACH Peroxidase OS=Phanerochaete chrysosporium OX=5306 GN=mnp2 PE=3 SV=1 ...
Phenol Phanerochaete chrysosporium Cultivation Heat and pressure treatment Lyophilization Biosorption Highlights. • Enhanced ... Singhal V, Kumar A, Rai JPN (2005) Bioremediation of pulp and paper mill effluent with Phanerochaete chrysosporium. J Environ ... Wu J, Yu H-Q (2007) Biosorption of 2,4-dichlorophenol by immobilized white-rot fungus Phanerochaete chrysosporium from aqueous ... Iqbal M, Saeed A (2007) Biosorption of reactive dye by loofa sponge-immobilized fungal biomass of Phanerochaete chrysosporium. ...
Timeline for Species Basidomycetes fungus (Phanerochaete chrysosporium) [TaxId:5306] from d.16.1.1 Flavoprotein domain of ... PDB entries in Species: Basidomycetes fungus (Phanerochaete chrysosporium) [TaxId: 5306]:. *Domain(s) for 1kdg: *. Domain ... Lineage for Species: Basidomycetes fungus (Phanerochaete chrysosporium) [TaxId: 5306]. *Root: SCOPe 2.06 *. Class d: Alpha and ... More info for Species Basidomycetes fungus (Phanerochaete chrysosporium) [TaxId:5306] from d.16.1.1 Flavoprotein domain of ...
A novel extracellular multicopper oxidase from Phanerochaete chrysosporium with ferroxidase activity. XML: View XML. Report an ... Whole transcriptome shotgun sequencing (RNA-seq) analyses based on the v.2.2 P. chrysosporium genome identified 356 genes whose ... We grew Phanerochaete chrysosporium on solid spruce wood and included oxidant-sensing beads bearing the fluorometric dye BODIPY ... Regulation of Gene Expression during the Onset of Ligninolytic Oxidation by Phanerochaete chrysosporium on Spruce Wood ...
A new antibiotic designated TMC-69 has been isolated from the fermentation broth of a fungal strain Chrysosporium sp. TC 1068. ... TMC-69, a new antitumor antibiotic with Cdc25A inhibitory activity, produced by Chrysosporium sp. TC1068. Taxonomy, ...
Phenotypic classes of phenoloxidase-negative mutants of the lignin-degrading fungus Phanerochaete chrysosporium.. R Liwicki, A ... This paper reports the isolation of phenoloxidase-negative mutants of the white-rot fungus Phanerochaete chrysosporium and the ... Phenotypic classes of phenoloxidase-negative mutants of the lignin-degrading fungus Phanerochaete chrysosporium. ... Phenotypic classes of phenoloxidase-negative mutants of the lignin-degrading fungus Phanerochaete chrysosporium. ...
Family 7 Cellobiohydrolases from Phanerochaete Chrysosporium: Crystal Structure of the Catalytic Module of Cel7D (Cbh58) at ... Cellobiohydrolase Cel7D (CBH 58) from Phanerochaete chrysosporium. Catalytic module at 1.32 Angstrom resolution. ...
Family 7 Cellobiohydrolases from Phanerochaete Chrysosporium: Crystal Structure of the Catalytic Module of Cel7D (Cbh58) at ... Cellobiohydrolase Cel7D (CBH 58) from Phanerochaete chrysosporium. Catalytic module at 1.32 Angstrom resolution. ...
Pease E.A., Tien M. (1991) Lignin-Degrading Enzymes from the Filamentous Fungus Phanerochaete chrysosporium. In: Dordick J.S. ( ...
... derivatives added into the growth medium were decolorized during submerged cultivation of Phanerochaete chrysosporium. The ... Kos N., Perdih A.: Polyoxirane distribution in aPhanerochaete chrysosporium culture.Folia Microbiol. 44, 527-529 (1999). ... Verma P., Madamwar D.: Decolorization of synthetic textile dyes by lignin peroxidase ofPhanerochaete chrysosporium.Folia ... Podgornik H., Grgič I., Perdih A.: Decolorization rate of dyes using lignin peroxidases ofPhanerochaete chrysosporium. ...
In recent years, the Chrysosporium anamorph of Nannizziopsis vriesii (CANV), Chrysosporium guarroi, Chrysosporium ophiodiicola ... Chrysosporium longisporum has been reclassified as Paranannizziopsis longispora. N. guarroi causes yellow fungus disease, a ... Molecular characterization of reptile pathogens currently known as members of the chrysosporium anamorph of nannizziopsis ... and Chrysosporium species have been reported as the causes of dermal or deep lesions in reptiles. These infections are ...
Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767.. C Srinivasan, T M Dsouza, K ... It has been widely reported that the white rot basidiomycete Phanerochaete chrysosporium, unlike most other white rot fungi, ... Our results showed that P. chrysosporium BKM-F1767 produces extracellular laccase in a defined culture medium containing ... Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767. ...
Chrysosporin, a new inhibitor of 3-hydroxy-3-methylglutaryl coenzyme A reductase produced by Chrysosporium pannorum PARK J. K. ... Pannorin, a new 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor produced by Chrysosporium pannorum OGAWA H. ... RPR113228, a novel farnesyl protein transferase inhibitor produced by Chrysosporium lobatum VAN DER PYL D. ... Structure of TMC-69, a new antitumor antibiotics from Chrysosporium sp. TC 1068 KOHNO J. ...
Two glucose oxidases have been reported in P. chrysosporium cultures: glucose 1-oxidase from P. chrysosporium ME-446 and ... chrysosporium, Pchr37396. The transcript accumulation of the P. chrysosporium gene was modest (NLB/RB ratio of 1.51), and no ... The P. chrysosporium v2.1 genome contains at least 13 sequences belonging to family 61, and six of these genes are clearly ... The P. chrysosporium pyranose 2-oxidase transcript patterns are similar to lignin peroxidases and GLX, supporting a role in ...
Under secondary metabolic conditions, the white-rot basidiomycete Phanerochaete chrysosporium degraded 2,7-dichlorodibenzo-p- ... Under secondary metabolic conditions, the white-rot basidiomycete Phanerochaete chrysosporium degraded 2,7-dichlorodibenzo-p- ... Under secondary metabolic conditions the white rot basidiomycete Phanerochaete chrysosporium rapidly mineralizes 2,4,5- ... Phanerochaete chrysosporium, and its involvement in the degradation of a lignin model compound. Agricultural and biological ...
... chain reaction of the extracellular flavocytochrome cellobiose dehydrogenase from the basidiomycete Phanerochaete chrysosporium ...
Phanerochaete chrysosporium in the form of pellet was made in this paper to examine the effects of physical and chemical ... P.chrysosporium mycelial pellets in the biosorption of Pb 2+ from aqueous solution,the uptake reached the maximum when the ... The research on the growth of Phanerochaete chrysosporium in the form of pellet was made in this paper to examine the effects ... The Effects of Physical and Chemical Conditions on Forming Mycelial Pellet of Phanerochaete chrysosporium and Biosorption of ...
Biodegradation of 2,4-dichlorophenol in a fluidized bed reactor with immobilized Phanerochaete chrysosporium Xiao-ming Li Xiao- ... In continuous bioreactor test, the kinetic behavior of the Phanerochaete chrysosporium immobilized on loofa sponge was found to ... The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4- ... The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP ...
... chrysosporium. The activity of laccase and lignin peroxidase excreted by A. oryzae was higher than that by P. chrysosporium in ... A comparative study on the degradation of gallic acid by Aspergillus oryzae and Phanerochaete chrysosporium Danzhao Guo Danzhao ... The present work aimed to compare the effect of Aspergillus oryzae 5992 and Phanerochaete chrysosporium 40719 on degradation of ... A comparative study on the degradation of gallic acid by Aspergillus oryzae and Phanerochaete chrysosporium. Water Sci Technol ...
Phanerochaete chrysosporium) for use in research, enzyme assays and in vitro diagnostic analysis. ... Recombinant from Phanerochaete chrysosporium Specificity: Hydrolysis of terminal non-reducing β-D-glucosidic bonds releasing β- ... High purity recombinant β-Glucosidase (Phanerochaete chrysosporium) for use in research, biochemical enzyme assays and in vitro ...
The results Underscore the rich genetic diversity and complexity of P. chrysosporiums extracellular enzyme systems. Published ... The white-rot basidiomycete Phanerochaete chrysosporium employs extracellular enzymes to completely degrade the major polymers ... Computational analysis of the Phanerochaete chrysosporium v2.0 genome database and mass spectrometry identification of peptides ... Computational analysis of the Phanerochaete chrysosporium v2.0 genome database and mass spectrometry identification of peptides ...
The white-rot fungus Phanerochaete chrysosporium possesses biodegradative capabilities of polychlorinated dibenzo-p-dioxins ( ... Oxidation of dibenzo-p-dioxin by lignin peroxidase from the basidiomycete Phanerochaete chrysosporium.. *D K Joshi, M H Gold ... P450 monooxygenases (P450ome) of the model white rot fungus Phanerochaete chrysosporium.. *Khajamohiddin Syed, Jagjit S Yadav ... Degradation of 2,7-dichlorodibenzo-p-dioxin by the lignin-degrading basidiomycete Phanerochaete chrysosporium.. *K Valli, H ...
... chrysosporium. Genes encoding manganese peroxidase numbered 13 and five in C. subvermispora and P. chrysosporium, respectively ... Phanerochaete chrysosporium simultaneously degrades lignin and cellulose, whereas the closely related species, Ceriporiopsis ... MS identified MnP proteins in C. subvermispora culture filtrates, but none in P. chrysosporium cultures. These results support ... Two C. subvermispora genes were predicted to encode peroxidases structurally similar to P. chrysosporium lignin peroxidase and ...
Kuan, I. C., & Tien, M. (1993). Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium: ... Kuan, IC & Tien, M 1993, Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium: Activity in ... Mn peroxidases are H2O2-utilizing hemeproteins secreted by the lignin-degrading fungus Phanerochaete chrysosporium. We show ... T1 - Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium. T2 - Activity in the absence of ...
  • Phenotypic classes of phenoloxidase-negative mutants of the lignin-degrading fungus Phanerochaete chrysosporium. (asm.org)
  • This paper reports the isolation of phenoloxidase-negative mutants of the white-rot fungus Phanerochaete chrysosporium and the results of a survey of idiophasic functions among these mutants. (asm.org)
  • The ∼30-Mb haploid genome of the most intensively studied white rot fungus, Phanerochaete chrysosporium , has been sequenced by the U.S. Department of Energy's Joint Genome Institute. (asm.org)
  • Degradation of the BTEX (benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes) group of organopollutants by the white-rot fungus Phanerochaete chrysosporium was studied. (eurekamag.com)
  • The white-rot fungus Phanerochaete chrysosporium possesses biodegradative capabilities of polychlorinated dibenzo-p-dioxins (PCDDs). (semanticscholar.org)
  • P450 monooxygenases (P450ome) of the model white rot fungus Phanerochaete chrysosporium. (semanticscholar.org)
  • Enzymatic properties of cytochrome P450 catalyzing 3'-hydroxylation of naringenin from the white-rot fungus Phanerochaete chrysosporium. (semanticscholar.org)
  • Mn peroxidases are H 2 O 2 -utilizing hemeproteins secreted by the lignin-degrading fungus Phanerochaete chrysosporium. (elsevier.com)
  • abstract = "Mn peroxidases are H2O2-utilizing hemeproteins secreted by the lignin-degrading fungus Phanerochaete chrysosporium. (elsevier.com)
  • The practice of exposing liquid cultures of the white-rot fungus Phanerochaete chrysosporium to a pure oxygen atmosphere under conditions of nutrient starvation has been widely adopted to induce lignin peroxidase (LiP) synthesis. (microbiologyresearch.org)
  • We examined gene expression patterns in the lignin-degrading fungus Phanerochaete chrysosporium when it colonizes hybrid poplar (Populus alba × tremula) and syringyl (S)-rich transgenic derivatives. (umn.edu)
  • In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium . (conicyt.cl)
  • The aerobic biodegradability of the high explosive CL-20 by activated sludge and the white rot fungus Phanerochaete chrysosporium has been investigated. (mdpi.com)
  • For biological pretreatment, grass was incubated for 3 weeks with the white-rot fungus, Phanerochaete chrysosporium . (ncsu.edu)
  • The white-rot fungus Phanerochaete chrysosporium is among the small group of fungi that can degrade lignin to carbon dioxide while leaving the crystalline cellulose untouched. (beds.ac.uk)
  • We report here the cloning of an aryl-alcohol dehydrogenase cDNA from the white-rot fungus Phanerochaete chrysosporium , its expression in Escherichia coli and the biochemical characterization of the encoded GST and His 6 tagged protein. (beds.ac.uk)
  • In this work, we have characterized the biochemical properties of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium . (beds.ac.uk)
  • The white-rot fungus Phanerochaete chrysosporium encodes 149 cytochrome P450 (CYP) genes in its genome. (sciepub.com)
  • The enzyme from the white rot fungus Phanerochaete chrysosporium is unusual in having two redoxin domains, one containing a flavin and the other a protoheme group. (qmul.ac.uk)
  • Comparison of ligninase-I and peroxidase-M2 from the white-rot fungus Phanerochaete chrysosporium. (genome.jp)
  • Prior to this study, one report demonstrated that the 6:2 FTOH biotransformation by the wood‐decaying fungus, Phanerochaete chrysosporium, generated more polyfluoroalkyl substances, such as 5:3 acid (F(CF2)5CH2CH2COOH), and diverted away from producing the highly stable perfluorocarboxylic acids (PFCAs). (deepdyve.com)
  • Demonstration of Laccase in the White Rot Basidiomycete Phanerochaete chrysosporium BKM-F1767. (asm.org)
  • It has been widely reported that the white rot basidiomycete Phanerochaete chrysosporium, unlike most other white rot fungi, does not produce laccase, an enzyme implicated in lignin biodegradation. (asm.org)
  • The wood decay basidiomycete Phanerochaete chrysosporium was grown under standard ligninolytic or cellulolytic conditions and subjected to whole-genome expression microarray analysis and liquid chromatography-tandem mass spectrometry of extracellular proteins. (asm.org)
  • Degradation of 2,7-dichlorodibenzo-p-dioxin by the lignin-degrading basidiomycete Phanerochaete chrysosporium. (semanticscholar.org)
  • Oxidation of dibenzo-p-dioxin by lignin peroxidase from the basidiomycete Phanerochaete chrysosporium. (semanticscholar.org)
  • Transcriptional analysis of β-glucosidase gene ( bgl ) and cellobiose dehydrogenase gene ( cdh ) in relation to cellobiose metabolism in the basidiomycete Phanerochaete chrysosporium was performed using real-time quantitative RT-PCR. (oup.com)
  • Molecular biology of the lignin-degrading basidiomycete Phanerochaete chrysosporium. (naver.com)
  • The crystal structure of the catalytic domain of a glycoside hydrolase family 6 cellobiohydrolase from the basidiomycete P. chrysosporium was solved in apo and cellobiose-liganded forms at 1.2 and 2.1 Å resolution, respectively. (iucr.org)
  • Phanerochaete chrysosporium simultaneously degrades lignin and cellulose, whereas the closely related species, Ceriporiopsis subvermispora, also depolymerizes lignin but may do so with relatively little cellulose degradation. (pnas.org)
  • Production of ligninases and degradation of lignin in agitated submerged cultures of Phanerochaete chrysosporium . (springer.com)
  • The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP degradation was examined. (iwaponline.com)
  • The present work aimed to compare the effect of Aspergillus oryzae 5992 and Phanerochaete chrysosporium 40719 on degradation of different concentrations of GA. The A. oryzae grew well and achieved a GA removal rate up to 99% in media containing 1-4% GA, much higher than P. chrysosporium . (iwaponline.com)
  • Differential expression in Phanerochaete chrysosporium of membrane-associated proteins relevant to lignin degradation. (semanticscholar.org)
  • 1997), some white rot fungi ( i.e.Phanerochaete chrysosporium ) do not produce this enzyme, suggesting that it may not be absolutely required for lignin degradation (Hatakka, 1994). (conicyt.cl)
  • Genes encoding manganese peroxidase numbered 13 and five in C. subvermispora and P. chrysosporium , respectively. (pnas.org)
  • Two C. subvermispora genes were predicted to encode peroxidases structurally similar to P. chrysosporium lignin peroxidase and, following heterologous expression in Escherichia coli , the enzymes were shown to oxidize high redox potential substrates, but not Mn 2+ . (pnas.org)
  • In addition to P. chrysosporium , multiple ligninolytic peroxidase isozymes and their corresponding genes have been identified in several efficient lignin-degrading fungi ( 4 ). (pnas.org)
  • Polyoxyethylene stimulates lignin peroxidase production in Phanerochaete chrysosporium . (springer.com)
  • Production of managanese-dependent peroxidase in a new solid-state bioreactor by Phanerochaete chrysosporium grown on wood shavings. (springer.com)
  • The activity of laccase and lignin peroxidase excreted by A. oryzae was higher than that by P. chrysosporium in the presence of GA. Based on the results of high-performance liquid chromatography-electrospray ionization-mass spectrometry, three relevant intermediate metabolites were determined as progallin A, methyl gallate, and pyrogallic acid, implying that A. oryzae could not degrade GA unless the carboxyl in the molecule was protected or removed. (iwaponline.com)
  • Kuan, IC & Tien, M 1993, ' Glyoxylate-supported reactions catalyzed by Mn peroxidase of Phanerochaete chrysosporium: Activity in the absence of added hydrogen peroxide ', Archives of Biochemistry and Biophysics , vol. 302, no. 2, pp. 447-454. (elsevier.com)
  • Johjima, T , Wariishi, H & Tanaka, H 2002, ' Veratryl alcohol binding sites of lignin peroxidase from Phanerochaete chrysosporium ', Journal of Molecular Catalysis - B Enzymatic , 巻. (elsevier.com)
  • An amperometric enzyme sensor for rapid and simultaneous detection of the lignin-degrading peroxidase activities secreted by Phanerochaete chrysosporium was developed, using H 2O 2, hydroquinone and veratryl alcohol as substrates. (naver.com)
  • Les enzymes antioxidants tels que superoxide dismutase, ascorbate peroxidase, glutathione réductase, et catalase sont moins actifs en présence de cadmium. (wikipedia.org)
  • A protocol for identification and differentiation of allelic variants of the lignin peroxidase gene in Phanerochaete chrysosporium was developed and is presented. (readabstracts.com)
  • This protocol allows rapid identification of lignin peroxidase genes and alleles and is useful for classifying P. chrysosporium strains. (readabstracts.com)
  • P. chrysosporium P450ome, the largest known todate among fungi, is characterized by tandem gene clusters and multigene families. (biomedcentral.com)
  • Comparative transcriptome and secretome analysis of wood decay fungi Postia placenta and Phanerochaete chrysosporium. (naver.com)
  • Some of the most common reported fungal diseases in reptiles are yellow fungus disease in lizards and snake fungal disease in snakes, caused by keratinophilic ascomycetous fungi of the family Onygenaceae (Eurotiomycetes: Onygenales), identified as a member of the Chrysosporium anamorph of the Nannizziopsis vriesii complex (CANV) ( 3 - 6 ). (asm.org)
  • Many reports so far have demonstrated that white-rot fungi, such as Phanerochaete chrysosporium, Trametes versicolor , Pleurotus ostreatus, Ganoderma spp. (hindawi.com)
  • In addition, the C. subvermispora genome contains at least seven genes predicted to encode laccases, whereas the P. chrysosporium genome contains none. (pnas.org)
  • Whole transcriptome shotgun sequencing (RNA-seq) analyses based on the v.2.2 P. chrysosporium genome identified 356 genes whose transcripts accumulated to relatively high levels at 96 h and were at least four times the levels found at 40 h. (usda.gov)
  • Our initial P450 cloning efforts in P. chrysosporium and its subsequent whole genome sequencing have revealed an extraordinary P450 repertoire ("P450ome") containing at least 150 P450 genes with yet unknown function. (biomedcentral.com)
  • Clan-level phylogenetic comparison revealed that P. chrysosporium P450 families fall under 11 fungal clans and the majority of these multigene families appear to have evolved locally in this genome from their respective progenitor genes, as a result of extensive gene duplications and rearrangements. (biomedcentral.com)
  • A functional analysis of the 12 PcCYP genes targeting the coding sequences of these P. chrysosporium P450 cDNAs as probes was conducted. (sciepub.com)
  • In recent years, the Chrysosporium anamorph of Nannizziopsis vriesii (CANV), Chrysosporium guarroi, Chrysosporium ophiodiicola, and Chrysosporium species have been reported as the causes of dermal or deep lesions in reptiles. (gc.ca)
  • Chrysosporium keratinophilum is regarded by some mycologists as the asexual state, or anamorph, however others argue that this is only the asexual state of Aphanoascus keratinophilus. (wikipedia.org)
  • The cycloheximide-resistant isolate was strongly keratinolytic and identified as a Chrysosporium anamorph of Nannizziopsis vriesii ( 1 , 2 ). (cdc.gov)
  • Phanerochaete chrysosporium simultaneously degrades cellulose, hemicellulose, and lignin, whereas a few others such as the closely related polypore species, Ceriporiopsis subvermispora , have the ability to remove lignin in advance of cellulose. (pnas.org)
  • Our results showed that P. chrysosporium BKM-F1767 produces extracellular laccase in a defined culture medium containing cellulose (10 g/liter) and either 2.4 or 24 mM ammonium tartrate. (asm.org)
  • High amounts of cellobiose:quinone oxidoreductase (3.33-3.99 IU/mL of peak value) and cellobiose dehydrogenase (0.04-0.2 IU/mL of peak value) were measured when P. chrysosporium was grown on a medium containing cellulose. (ncsu.edu)
  • This work discovered that the mixture of glucose and cellulose as a carbon source favored high co-production of ligninolytic enzymes by P. chrysosporium. (ncsu.edu)
  • Fermentation of 100 g of rice straw feedstock containing 35.7 g of glucan (mainly in the form of cellulose) by cultivation with P. chrysosporium for 15 days in the media optimized by response surface methodology was resulted in a yield of 29.0 g of glucan that had an enzymatic digestibility of 64.9% of the theoretical maximum glucose yield. (elsevier.com)
  • MS identified MnP proteins in C. subvermispora culture filtrates, but none in P. chrysosporium cultures. (pnas.org)
  • Corneal scrapings demonstrated numerous septate hyphae, and specimen cultures were positive for Chrysosporium sp. (dovepress.com)
  • We grew Phanerochaete chrysosporium on solid spruce wood and included oxidant-sensing beads bearing the fluorometric dye BODIPY 581/591 in the cultures. (usda.gov)
  • Researchers illustrate the use of cultures derived from a single basidiospore of Phanerochaete chrysosporium for genetic analysis of transposons. (readabstracts.com)
  • abstract = "Phanerochaete chrysosporium is a wood-rot fungus that is capable of degrading lignin via its lignolytic system. (elsevier.com)
  • To provide a more complete view of gene expression, we report here transcriptome and secretome analyses of P. chrysosporium grown under defined ligninolytic and cellulolytic conditions. (asm.org)
  • The effect of altering the carbon source in the growing environment was investigated relative to the production of ligninolytic enzymes by Phanerochaete chrysosporium. (ncsu.edu)
  • The colonial appearance of A. fulvescens generally mimics that of species in the genus Trichophyton and Chrysosporium while under the microscope the appearance of the organism resembles that of the genus Aspergillus. (wikipedia.org)
  • The evolved P450 diversity is consistent with the known vast biotransformation potential of P. chrysosporium . (biomedcentral.com)
  • This study aimed at elucidating the functions of P. chrysosporium 's cytochrome P450 (PcCYP) gene repertoire using the polyclic aromatic hydrocarbon (PAH) benzo(a)pyrene (BaP) as a substrate. (sciepub.com)
  • A cDNA microarray system targeting 133 P. chrysosporium P450 cDNAs as probes was used to investigate the differential expression patterns of PcCYPs gene diversity in response to this PAH. (sciepub.com)
  • Fluorescein derivatives added into the growth medium were decolorized during submerged cultivation of Phanerochaete chrysosporium . (springer.com)
  • A new antibiotic designated TMC-69 has been isolated from the fermentation broth of a fungal strain Chrysosporium sp. (nih.gov)
  • The direct oxidation of RNase and Poly R by MnP2 (VP) is in sharp contrast with redox mediator-dependent oxidation of these polymers by LiP from Phanerochaete chrysosporium . (portlandpress.com)
  • Transcript profiles of Phanerochaete chrysosporium grown on carbon-limited medium, nitrogen-limited medium and replete medium. (omicsdi.org)
  • Study done to identify and characterize the glucose-oxidizing activity of Phanerochaete chrysosporium grown on solid substrate is reported. (readabstracts.com)
  • High purity recombinant β-Glucosidase ( Phanerochaete chrysosporium ) for use in research, biochemical enzyme assays and in vitro diagnostic analysis. (megazyme.com)
  • In summary, the C. subvermispora genetic inventory and expression patterns exhibit increased oxidoreductase potential and diminished cellulolytic capability relative to P. chrysosporium . (pnas.org)
  • Comparison of static and shake culture in the decolorization of textile dyes and dye effluents by Phanerochaete chrysosporium . (springer.com)
  • In this paper, it was also applied in the detection of lignin-degrading peroxidases in compost bioremediation using P. chrysosporium, showing considerable advantages. (naver.com)
  • Peptides corresponding to a total of 18 oxidoreductases were identified in medium consisting of biomass from line 64 or 82 (85 mol% S) but not in the parental clone (65 mol% S). These results demonstrate that P. chrysosporium gene expression patterns are substantially influenced by lignin composition. (umn.edu)
  • Gene Expression Profiling and Enzymatic Function of Phanerochaete Chrysosporium Cytochrome P4. (sciepub.com)
  • Phanerochaete chrysosporium degrades CL-20 in the presence of supplementary carbon and nitrogen sources. (mdpi.com)
  • Our analysis showed that P. chrysosporium P450ome could be classified into 12 families and 23 sub-families and is characterized by the presence of multigene families. (biomedcentral.com)
  • Two novel arabinofuranosidases, Abn7 and Abf3 from Chrysosporium lucknowense (C1), belonging to the glycoside hydrolase family 43 and 51 were purified and characterized. (naver.com)
  • The biosorption of phenol on non-living lyophilized mycelial pellets of Phanerochaete chrysosporium cultivated in liquid medium of various compositions was studied in batch biosorption system. (springer.com)
  • In continuous bioreactor test, the kinetic behavior of the Phanerochaete chrysosporium immobilized on loofa sponge was found to follow the Monod equation. (iwaponline.com)
  • The new compounds were found to inhibit β-glucosidase from Phanerochaete chrysosporium and α-glucosidase from Aspergillus niger , with K i values in the low micro- to nanomolar range. (rsc.org)
  • Phanerochaete chrysosporium , the model white rot basidiomycetous fungus, has the extraordinary ability to mineralize (to CO 2 ) lignin and detoxify a variety of chemical pollutants. (biomedcentral.com)
  • To investigate the basis for selective ligninolysis, we conducted comparative genome analysis of C. subvermispora and P. chrysosporium . (pnas.org)