Chromosomes artificial p1 bacteriophage. Medical search

Viruses whose hosts are bacterial cells.

In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)

Any method used for determining the location of and relative distances between genes on a chromosome.

Virulent bacteriophage and type species of the genus T4-like phages, in the family MYOVIRIDAE. It infects E. coli and is the best known of the T-even phages. Its virion contains linear double-stranded DNA, terminally redundant and circularly permuted.

A temperate inducible phage and type species of the genus lambda-like viruses, in the family SIPHOVIRIDAE. Its natural host is E. coli K12. Its VIRION contains linear double-stranded DNA with single-stranded 12-base 5' sticky ends. The DNA circularizes on infection.

Viruses whose host is Escherichia coli.

Virulent bacteriophage and type species of the genus T7-like phages, in the family PODOVIRIDAE, that infects E. coli. It consists of linear double-stranded DNA, terminally redundant, and non-permuted.

Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.

The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. Upon induction (VIRUS ACTIVATION) by various agents, such as ultraviolet radiation, the phage is released, which then becomes virulent and lyses the bacterium.

The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.

A series of 7 virulent phages which infect E. coli. The T-even phages T2, T4; (BACTERIOPHAGE T4), and T6, and the phage T5 are called "autonomously virulent" because they cause cessation of all bacterial metabolism on infection. Phages T1, T3; (BACTERIOPHAGE T3), and T7; (BACTERIOPHAGE T7) are called "dependent virulent" because they depend on continued bacterial metabolism during the lytic cycle. The T-even phages contain 5-hydroxymethylcytosine in place of ordinary cytosine in their DNA.

Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.

A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. It frequently causes a mutation by interrupting the continuity of the bacterial OPERON at the site of insertion.

Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.

The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed)

A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.

A species of gram-negative, facultatively anaerobic, rod-shaped bacteria (GRAM-NEGATIVE FACULTATIVELY ANAEROBIC RODS) commonly found in the lower part of the intestine of warm-blooded animals. It is usually nonpathogenic, but some strains are known to produce DIARRHEA and pyogenic infections. Pathogenic strains (virotypes) are classified by their specific pathogenic mechanisms such as toxins (ENTEROTOXIGENIC ESCHERICHIA COLI), etc.

Deoxyribonucleic acid that makes up the genetic material of viruses.

Very long DNA molecules and associated proteins, HISTONES, and non-histone chromosomal proteins (CHROMOSOMAL PROTEINS, NON-HISTONE). Normally 46 chromosomes, including two sex chromosomes are found in the nucleus of human cells. They carry the hereditary information of the individual.

Virulent bacteriophage and sole member of the genus Cystovirus that infects Pseudomonas species. The virion has a segmented genome consisting of three pieces of doubled-stranded DNA and also a unique lipid-containing envelope.

The orderly segregation of CHROMOSOMES during MEIOSIS or MITOSIS.

A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.

The type species of the genus MICROVIRUS. A prototype of the small virulent DNA coliphages, it is composed of a single strand of supercoiled circular DNA, which on infection, is converted to a double-stranded replicative form by a host enzyme.

A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.

Actual loss of portion of a chromosome.

A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.

Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.

Proteins found in any species of virus.

The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.

A specific pair GROUP C CHROMSOMES of the human chromosome classification.

A species of temperate bacteriophage in the genus P2-like viruses, family MYOVIRIDAE, which infects E. coli. It consists of linear double-stranded DNA with 19-base sticky ends.

A specific pair of GROUP C CHROMSOMES of the human chromosome classification.

A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.

Any detectable and heritable change in the genetic material that causes a change in the GENOTYPE and which is transmitted to daughter cells and to succeeding generations.

Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of PLANTS.

Temperate bacteriophage of the genus INOVIRUS which infects enterobacteria, especially E. coli. It is a filamentous phage consisting of single-stranded DNA and is circularly permuted.

Structures within the nucleus of fungal cells consisting of or containing DNA, which carry genetic information essential to the cell.

Viruses whose nucleic acid is DNA.

The medium-sized, submetacentric human chromosomes, called group C in the human chromosome classification. This group consists of chromosome pairs 6, 7, 8, 9, 10, 11, and 12 and the X chromosome.

Bacteriophage in the genus T7-like phages, of the family PODOVIRIDAE, which is very closely related to BACTERIOPHAGE T7.

A species of temperate bacteriophage in the genus P1-like viruses, family MYOVIRIDAE, which infects E. coli. It is the largest of the COLIPHAGES and consists of double-stranded DNA, terminally redundant, and circularly permuted.

A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.

A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.

A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.

The alignment of CHROMOSOMES at homologous sequences.

A technique of bacterial typing which differentiates between bacteria or strains of bacteria by their susceptibility to one or more bacteriophages.

Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of MAMMALS.

A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.

A specific pair of GROUP B CHROMOSOMES of the human chromosome classification.

A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.

The human male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans.

A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.

A specific pair of GROUP F CHROMOSOMES of the human chromosome classification.

Clinical conditions caused by an abnormal chromosome constitution in which there is extra or missing chromosome material (either a whole chromosome or a chromosome segment). (from Thompson et al., Genetics in Medicine, 5th ed, p429)

Viruses whose host is Salmonella. A frequently encountered Salmonella phage is BACTERIOPHAGE P22.

DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.

Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.

A family of BACTERIOPHAGES and ARCHAEAL VIRUSES which are characterized by long, non-contractile tails.

The human female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in humans.

The large, metacentric human chromosomes, called group A in the human chromosome classification. This group consists of chromosome pairs 1, 2, and 3.

A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.

A technique for visualizing CHROMOSOME ABERRATIONS using fluorescently labeled DNA probes which are hybridized to chromosomal DNA. Multiple fluorochromes may be attached to the probes. Upon hybridization, this produces a multicolored, or painted, effect with a unique color at each site of hybridization. This technique may also be used to identify cross-species homology by labeling probes from one species for hybridization with chromosomes from another species.

One of the two pairs of human chromosomes in the group B class (CHROMOSOMES, HUMAN, 4-5).

The functional hereditary units of VIRUSES.

A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.

Bacteriophages whose genetic material is RNA, which is single-stranded in all except the Pseudomonas phage phi 6 (BACTERIOPHAGE PHI 6). All RNA phages infect their host bacteria via the host's surface pili. Some frequently encountered RNA phages are: BF23, F2, R17, fr, PhiCb5, PhiCb12r, PhiCb8r, PhiCb23r, 7s, PP7, Q beta phage, MS2 phage, and BACTERIOPHAGE PHI 6.

Mapping of the KARYOTYPE of a cell.

A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.

A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.

Rupture of bacterial cells due to mechanical force, chemical action, or the lytic growth of BACTERIOPHAGES.

Viruses whose host is Staphylococcus.

The short, submetacentric human chromosomes, called group E in the human chromosome classification. This group consists of chromosome pairs 16, 17, and 18.

The co-inheritance of two or more non-allelic GENES due to their being located more or less closely on the same CHROMOSOME.

Bacteriophage and type species in the genus Tectivirus, family TECTIVIRIDAE. They are specific for Gram-negative bacteria.

Viruses whose host is Pseudomonas. A frequently encountered Pseudomonas phage is BACTERIOPHAGE PHI 6.

A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.

A specific pair of GROUP F CHROMOSOMES of the human chromosome classification.

Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.

The process by which a DNA molecule is duplicated.

Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.

The medium-sized, acrocentric human chromosomes, called group D in the human chromosome classification. This group consists of chromosome pairs 13, 14, and 15.

A family of bacteriophages which are characterized by short, non-contractile tails.

Extrachromosomal, usually CIRCULAR DNA molecules that are self-replicating and transferable from one organism to another. They are found in a variety of bacterial, archaeal, fungal, algal, and plant species. They are used in GENETIC ENGINEERING as CLONING VECTORS.

A category of nucleic acid sequences that function as units of heredity and which code for the basic instructions for the development, reproduction, and maintenance of organisms.

The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.

The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.

A type of chromosomal aberration involving DNA BREAKS. Chromosome breakage can result in CHROMOSOMAL TRANSLOCATION; CHROMOSOME INVERSION; or SEQUENCE DELETION.

Viruses whose host is Streptococcus.

The short, acrocentric human chromosomes, called group G in the human chromosome classification. This group consists of chromosome pairs 21 and 22 and the Y chromosome.

An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.

A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.

Aberrant chromosomes with no ends, i.e., circular.

Proteins found in the tail sections of DNA and RNA viruses. It is believed that these proteins play a role in directing chain folding and assembly of polypeptide chains.

The mechanisms of eukaryotic CELLS that place or keep the CHROMOSOMES in a particular SUBNUCLEAR SPACE.

The complete genetic complement contained in a DNA or RNA molecule in a virus.

The large, submetacentric human chromosomes, called group B in the human chromosome classification. This group consists of chromosome pairs 4 and 5.

A bacteriophage genus of the family LEVIVIRIDAE, whose viruses contain the short version of the genome and have a separate gene for cell lysis.

Deoxyribonucleic acid that makes up the genetic material of bacteria.

Enzymes that are part of the restriction-modification systems. They catalyze the endonucleolytic cleavage of DNA sequences which lack the species-specific methylation pattern in the host cell's DNA. Cleavage yields random or specific double-stranded fragments with terminal 5'-phosphates. The function of restriction enzymes is to destroy any foreign DNA that invades the host cell. Most have been studied in bacterial systems, but a few have been found in eukaryotic organisms. They are also used as tools for the systematic dissection and mapping of chromosomes, in the determination of base sequences of DNAs, and have made it possible to splice and recombine genes from one organism into the genome of another. EC 3.21.1.

Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)

The folding of an organism's DNA molecule into a compact, orderly structure that fits within the limited space of a CELL or VIRUS PARTICLE.

A dosage compensation process occurring at an early embryonic stage in mammalian development whereby, at random, one X CHROMOSOME of the pair is repressed in the somatic cells of females.

Genomes of temperate BACTERIOPHAGES integrated into the DNA of their bacterial host cell. The prophages can be duplicated for many cell generations until some stimulus induces its activation and virulence.

The adhesion of gases, liquids, or dissolved solids onto a surface. It includes adsorptive phenomena of bacteria and viruses onto surfaces as well. ABSORPTION into the substance may follow but not necessarily.

A multistage process that includes cloning, physical mapping, subcloning, determination of the DNA SEQUENCE, and information analysis.

The clear constricted portion of the chromosome at which the chromatids are joined and by which the chromosome is attached to the spindle during cell division.

A deoxyribonucleotide polymer that is the primary genetic material of all cells. Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. DNA, which consists of a polysugar-phosphate backbone possessing projections of purines (adenine and guanine) and pyrimidines (thymine and cytosine), forms a double helix that is held together by hydrogen bonds between these purines and pyrimidines (adenine to thymine and guanine to cytosine).

Deliberate breeding of two different individuals that results in offspring that carry part of the genetic material of each parent. The parent organisms must be genetically compatible and may be from different varieties or closely related species.

Specific loci on both the bacterial DNA (attB) and the phage DNA (attP) which delineate the sites where recombination takes place between them, as the phage DNA becomes integrated (inserted) into the BACTERIAL DNA during LYSOGENY.

A species of gram-positive bacteria that is a common soil and water saprophyte.

The outward appearance of the individual. It is the product of interactions between genes, and between the GENOTYPE and the environment.

Structures within the CELL NUCLEUS of insect cells containing DNA.

A subdiscipline of genetics which deals with the genetic mechanisms and processes of microorganisms.

A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.

A type of CELL NUCLEUS division, occurring during maturation of the GERM CELLS. Two successive cell nucleus divisions following a single chromosome duplication (S PHASE) result in daughter cells with half the number of CHROMOSOMES as the parent cells.

Any cell, other than a ZYGOTE, that contains elements (such as NUCLEI and CYTOPLASM) from two or more different cells, usually produced by artificial CELL FUSION.

A genus of filamentous bacteriophages of the family INOVIRIDAE. Organisms of this genus infect enterobacteria, PSEUDOMONAS; VIBRIO; and XANTHOMONAS.

Enzymes that catalyze DNA template-directed extension of the 3'-end of an RNA strand one nucleotide at a time. They can initiate a chain de novo. In eukaryotes, three forms of the enzyme have been distinguished on the basis of sensitivity to alpha-amanitin, and the type of RNA synthesized. (From Enzyme Nomenclature, 1992).

Structures which are contained in or part of CHROMOSOMES.

The short, metacentric human chromosomes, called group F in the human chromosome classification. This group consists of chromosome pairs 19 and 20.

The biosynthesis of RNA carried out on a template of DNA. The biosynthesis of DNA from an RNA template is called REVERSE TRANSCRIPTION.

The chromosomal constitution of cells which deviate from the normal by the addition or subtraction of CHROMOSOMES, chromosome pairs, or chromosome fragments. In a normally diploid cell (DIPLOIDY) the loss of a chromosome pair is termed nullisomy (symbol: 2N-2), the loss of a single chromosome is MONOSOMY (symbol: 2N-1), the addition of a chromosome pair is tetrasomy (symbol: 2N+2), the addition of a single chromosome is TRISOMY (symbol: 2N+1).

The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.

The phase of cell nucleus division following PROMETAPHASE, in which the CHROMOSOMES line up across the equatorial plane of the SPINDLE APPARATUS prior to separation.

A type of CELL NUCLEUS division by means of which the two daughter nuclei normally receive identical complements of the number of CHROMOSOMES of the somatic cells of the species.

Microscopy using an electron beam, instead of light, to visualize the sample, thereby allowing much greater magnification. The interactions of ELECTRONS with specimens are used to provide information about the fine structure of that specimen. In TRANSMISSION ELECTRON MICROSCOPY the reactions of the electrons that are transmitted through the specimen are imaged. In SCANNING ELECTRON MICROSCOPY an electron beam falls at a non-normal angle on the specimen and the image is derived from the reactions occurring above the plane of the specimen.

Proteins found in any species of bacterium.

The process of intracellular viral multiplication, consisting of the synthesis of PROTEINS; NUCLEIC ACIDS; and sometimes LIPIDS, and their assembly into a new infectious particle.

A single chain of deoxyribonucleotides that occurs in some bacteria and viruses. It usually exists as a covalently closed circle.

The functional hereditary units of BACTERIA.

A test used to determine whether or not complementation (compensation in the form of dominance) will occur in a cell with a given mutant phenotype when another mutant genome, encoding the same mutant phenotype, is introduced into that cell.

Method for measuring viral infectivity and multiplication in CULTURED CELLS. Clear lysed areas or plaques develop as the VIRAL PARTICLES are released from the infected cells during incubation. With some VIRUSES, the cells are killed by a cytopathic effect; with others, the infected cells are not killed but can be detected by their hemadsorptive ability. Sometimes the plaque cells contain VIRAL ANTIGENS which can be measured by IMMUNOFLUORESCENCE.

Separation of particles according to density by employing a gradient of varying densities. At equilibrium each particle settles in the gradient at a point equal to its density. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

The spatial arrangement of the atoms of a nucleic acid or polynucleotide that results in its characteristic 3-dimensional shape.

Proteins which bind to DNA. The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases.

The total relative probability, expressed on a logarithmic scale, that a linkage relationship exists among selected loci. Lod is an acronym for "logarithmic odds."

Variant forms of the same gene, occupying the same locus on homologous CHROMOSOMES, and governing the variants in production of the same gene product.

The outer protein protective shell of a virus, which protects the viral nucleic acid.

The record of descent or ancestry, particularly of a particular condition or trait, indicating individual family members, their relationships, and their status with respect to the trait or condition.

A variety of simple repeat sequences that are distributed throughout the GENOME. They are characterized by a short repeat unit of 2-8 basepairs that is repeated up to 100 times. They are also known as short tandem repeats (STRs).

Theoretical representations that simulate the behavior or activity of genetic processes or phenomena. They include the use of mathematical equations, computers, and other electronic equipment.

The property of objects that determines the direction of heat flow when they are placed in direct thermal contact. The temperature is the energy of microscopic motions (vibrational and translational) of the particles of atoms.

A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.

The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.

Enzymes which catalyze the hydrolases of ester bonds within DNA. EC 3.1.-.

An antibiotic first isolated from cultures of Streptomyces venequelae in 1947 but now produced synthetically. It has a relatively simple structure and was the first broad-spectrum antibiotic to be discovered. It acts by interfering with bacterial protein synthesis and is mainly bacteriostatic. (From Martindale, The Extra Pharmacopoeia, 29th ed, p106)

The genetic constitution of the individual, comprising the ALLELES present at each GENETIC LOCUS.

Discrete segments of DNA which can excise and reintegrate to another site in the genome. Most are inactive, i.e., have not been found to exist outside the integrated state. DNA transposable elements include bacterial IS (insertion sequence) elements, Tn elements, the maize controlling elements Ac and Ds, Drosophila P, gypsy, and pogo elements, the human Tigger elements and the Tc and mariner elements which are found throughout the animal kingdom.

The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.

A sequence of successive nucleotide triplets that are read as CODONS specifying AMINO ACIDS and begin with an INITIATOR CODON and end with a stop codon (CODON, TERMINATOR).

In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.

The sequential correspondence of nucleotides in one nucleic acid molecule with those of another nucleic acid molecule. Sequence homology is an indication of the genetic relatedness of different organisms and gene function.

A family of bacteriophages containing one genus (Cystovirus) with one member (BACTERIOPHAGE PHI 6).

A species of filamentous Pseudomonas phage in the genus INOVIRUS, family INOVIRIDAE.

Proteins that form the CAPSID of VIRUSES.

The possession of a third chromosome of any one type in an otherwise diploid cell.

The degree of similarity between sequences of amino acids. This information is useful for the analyzing genetic relatedness of proteins and species.

In bacteria, a group of metabolically related genes, with a common promoter, whose transcription into a single polycistronic MESSENGER RNA is under the control of an OPERATOR REGION.

Sequences of DNA or RNA that occur in multiple copies. There are several types: INTERSPERSED REPETITIVE SEQUENCES are copies of transposable elements (DNA TRANSPOSABLE ELEMENTS or RETROELEMENTS) dispersed throughout the genome. TERMINAL REPEAT SEQUENCES flank both ends of another sequence, for example, the long terminal repeats (LTRs) on RETROVIRUSES. Variations may be direct repeats, those occurring in the same direction, or inverted repeats, those opposite to each other in direction. TANDEM REPEAT SEQUENCES are copies which lie adjacent to each other, direct or inverted (INVERTED REPEAT SEQUENCES).

Ribonucleic acid that makes up the genetic material of viruses.

An order comprising three families of tailed bacteriophages: MYOVIRIDAE; PODOVIRIDAE; and SIPHOVIRIDAE.

DNA-dependent DNA polymerases found in bacteria, animal and plant cells. During the replication process, these enzymes catalyze the addition of deoxyribonucleotide residues to the end of a DNA strand in the presence of DNA as template-primer. They also possess exonuclease activity and therefore function in DNA repair.

The failure of homologous CHROMOSOMES or CHROMATIDS to segregate during MITOSIS or MEIOSIS with the result that one daughter cell has both of a pair of parental chromosomes or chromatids and the other has none.

Biologically active DNA which has been formed by the in vitro joining of segments of DNA from different sources. It includes the recombination joint or edge of a heteroduplex region where two recombining DNA molecules are connected.

Stable phosphorus atoms that have the same atomic number as the element phosphorus, but differ in atomic weight. P-31 is a stable phosphorus isotope.

DNA constructs that are composed of, at least, all elements, such as a REPLICATION ORIGIN; TELOMERE; and CENTROMERE, required for successful replication, propagation to and maintainance in progeny human cells. In addition, they are constructed to carry other sequences for analysis or gene transfer.

Large multiprotein complexes that bind the centromeres of the chromosomes to the microtubules of the mitotic spindle during metaphase in the cell cycle.

Enzymes that catalyze the incorporation of deoxyribonucleotides into a chain of DNA. EC 2.7.7.-.

The relationships of groups of organisms as reflected by their genetic makeup.

A terminal section of a chromosome which has a specialized structure and which is involved in chromosomal replication and stability. Its length is believed to be a few hundred base pairs.

The sum of the weight of all the atoms in a molecule.

The arrangement of two or more amino acid or base sequences from an organism or organisms in such a way as to align areas of the sequences sharing common properties. The degree of relatedness or homology between the sequences is predicted computationally or statistically based on weights assigned to the elements aligned between the sequences. This in turn can serve as a potential indicator of the genetic relatedness between the organisms.

Proteins that catalyze the unwinding of duplex DNA during replication by binding cooperatively to single-stranded regions of DNA or to short regions of duplex DNA that are undergoing transient opening. In addition DNA helicases are DNA-dependent ATPases that harness the free energy of ATP hydrolysis to translocate DNA strands.

The parts of a macromolecule that directly participate in its specific combination with another molecule.

A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.

The rate dynamics in chemical or physical systems.

Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.

A group of enzymes catalyzing the endonucleolytic cleavage of DNA. They include members of EC 3.1.21.-, EC 3.1.22.-, EC 3.1.23.- (DNA RESTRICTION ENZYMES), EC 3.1.24.- (DNA RESTRICTION ENZYMES), and EC 3.1.25.-.

Any of the covalently closed DNA molecules found in bacteria, many viruses, mitochondria, plastids, and plasmids. Small, polydisperse circular DNA's have also been observed in a number of eukaryotic organisms and are suggested to have homology with chromosomal DNA and the capacity to be inserted into, and excised from, chromosomal DNA. It is a fragment of DNA formed by a process of looping out and deletion, containing a constant region of the mu heavy chain and the 3'-part of the mu switch region. Circular DNA is a normal product of rearrangement among gene segments encoding the variable regions of immunoglobulin light and heavy chains, as well as the T-cell receptor. (Riger et al., Glossary of Genetics, 5th ed & Segen, Dictionary of Modern Medicine, 1992)

A single-stranded DNA-dependent RNA polymerase that functions to initiate, or prime, DNA synthesis by synthesizing oligoribonucleotide primers. EC 2.7.7.-.

Recombinases that insert exogenous DNA into the host genome. Examples include proteins encoded by the POL GENE of RETROVIRIDAE and also by temperate BACTERIOPHAGES, the best known being BACTERIOPHAGE LAMBDA.

Nucleoproteins, which in contrast to HISTONES, are acid insoluble. They are involved in chromosomal functions; e.g. they bind selectively to DNA, stimulate transcription resulting in tissue-specific RNA synthesis and undergo specific changes in response to various hormones or phytomitogens.

That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.

An increased tendency to acquire CHROMOSOME ABERRATIONS when various processes involved in chromosome replication, repair, or segregation are dysfunctional.

A microtubule structure that forms during CELL DIVISION. It consists of two SPINDLE POLES, and sets of MICROTUBULES that may include the astral microtubules, the polar microtubules, and the kinetochore microtubules.

Short sequences (generally about 10 base pairs) of DNA that are complementary to sequences of messenger RNA and allow reverse transcriptases to start copying the adjacent sequences of mRNA. Primers are used extensively in genetic and molecular biology techniques.

Genotypic differences observed among individuals in a population.

Proteins obtained from ESCHERICHIA COLI.

DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. Highly conserved sequences within the promoter include the Pribnow box in bacteria and the TATA BOX in eukaryotes.

Susceptibility of chromosomes to breakage leading to translocation; CHROMOSOME INVERSION; SEQUENCE DELETION; or other CHROMOSOME BREAKAGE related aberrations.

A genetic rearrangement through loss of segments of DNA or RNA, bringing sequences which are normally separated into close proximity. This deletion may be detected using cytogenetic techniques and can also be inferred from the phenotype, indicating a deletion at one specific locus.

A set of genes descended by duplication and variation from some ancestral gene. Such genes may be clustered together on the same chromosome or dispersed on different chromosomes. Examples of multigene families include those that encode the hemoglobins, immunoglobulins, histocompatibility antigens, actins, tubulins, keratins, collagens, heat shock proteins, salivary glue proteins, chorion proteins, cuticle proteins, yolk proteins, and phaseolins, as well as histones, ribosomal RNA, and transfer RNA genes. The latter three are examples of reiterated genes, where hundreds of identical genes are present in a tandem array. (King & Stanfield, A Dictionary of Genetics, 4th ed)

Macromolecular molds for the synthesis of complementary macromolecules, as in DNA REPLICATION; GENETIC TRANSCRIPTION of DNA to RNA, and GENETIC TRANSLATION of RNA into POLYPEPTIDES.

Electron microscopy involving rapid freezing of the samples. The imaging of frozen-hydrated molecules and organelles permits the best possible resolution closest to the living state, free of chemical fixatives or stains.

The properties of a pathogen that makes it capable of infecting one or more specific hosts. The pathogen can include PARASITES as well as VIRUSES; BACTERIA; FUNGI; or PLANTS.

Genetic loci associated with a QUANTITATIVE TRAIT.

The genetic constitution of individuals with respect to one member of a pair of allelic genes, or sets of genes that are closely linked and tend to be inherited together such as those of the MAJOR HISTOCOMPATIBILITY COMPLEX.

Treatment of diseases with biological materials or biological response modifiers, such as the use of GENES; CELLS; TISSUES; organs; SERUM; VACCINES; and humoral agents.

Change brought about to an organisms genetic composition by unidirectional transfer (TRANSFECTION; TRANSDUCTION, GENETIC; CONJUGATION, GENETIC, etc.) and incorporation of foreign DNA into prokaryotic or eukaryotic cells by recombination of part or all of that DNA into the cell's genome.

Disruption of the secondary structure of nucleic acids by heat, extreme pH or chemical treatment. Double strand DNA is "melted" by dissociation of the non-covalent hydrogen bonds and hydrophobic interactions. Denatured DNA appears to be a single-stranded flexible structure. The effects of denaturation on RNA are similar though less pronounced and largely reversible.

A chromosomal region 7p11.2 transcript map: its development and application to the study of EGFR amplicons in glioblastoma. (1/25)

Cumulative information available about the organization of amplified chromosomal regions in human tumors suggests that the amplification repeat units, or amplicons, can be of a simple or complex nature. For the former, amplified regions generally retain their native chromosomal configuration and involve a single amplification target sequence. For complex amplicons, amplified DNAs usually undergo substantial reorganization relative to the normal chromosomal regions from which they evolve, and the regions subject to amplification may contain multiple target sequences. Previous efforts to characterize the 7p11.2 epidermal growth factor receptor ) amplicon in glioblastoma have relied primarily on the use of markers positioned by linkage analysis and/or radiation hybrid mapping, both of which are known to have the potential for being inaccurate when attempting to order loci over relatively short (<1 Mb) chromosomal regions. Due to the limited resolution of genetic maps that have been established through the use of these approaches, we have constructed a 2-Mb bacterial and P1-derived artificial chromosome (BAC-PAC) contig for the EGFR region and have applied markers positioned on its associated physical map to the analysis of 7p11.2 amplifications in a series of glioblastomas. Our data indicate that EGFR is the sole amplification target within the mapped region, although there are several additional 7p11.2 genes that can be coamplified and overexpressed with EGFR. Furthermore, these results are consistent with EGFR amplicons retaining the same organization as the native chromosome 7p11.2 region from which they are derived. (+info)

The common retroviral insertion locus Dsi1 maps 30 kilobases upstream of the P1 promoter of the murine Runx3/Cbfa3/Aml2 gene. (2/25)

The Dsi1 locus was identified as a common integration site for Moloney murine leukemia virus (MLV) in rat thymic lymphomas, but previous efforts to identify a gene affected by these insertions were unsuccessful. We considered the Runx3 gene a potential candidate on the basis of genetic mapping which showed that Dsi1 and Runx3 are closely linked on mouse chromosome 4 and the precedent of the related Runx2 gene, which emerged recently as a Myc-collaborating gene activated by retroviral insertion in thymic lymphomas of CD2-MYC mice. We now report the physical mapping of the Dsi1 locus to a site 30 kb upstream of the distal (P1) promoter of the murine Runx3 gene. Comparison with the syntenic region of human chromosome 1 shows that the next gene is over 250 kb 5' to Runx3, suggesting that Runx3 may be the primary target of retroviral insertions at Dsi1. Screening of CD2-MYC lymphomas for rearrangements at Dsi1 revealed a tumor cell line harboring an MLV provirus at this locus, in the orientation opposite that of Runx3. Proviral insertion was associated with very high levels of expression of Runx3, with a preponderance of transcripts arising at the P1 promoter. These results confirm that Runx3 is a target of retroviral insertions at Dsi1 and indicate that Runx3 can act as an alternative to Runx2 as a Myc-collaborating gene in thymic lymphoma. (+info)

Genes in a refined Smith-Magenis syndrome critical deletion interval on chromosome 17p11.2 and the syntenic region of the mouse. (3/25)

Smith-Magenis syndrome (SMS) is a multiple congenital anomaly/mental retardation syndrome associated with behavioral abnormalities and sleep disturbance. Most patients have the same approximately 4 Mb interstitial genomic deletion within chromosome 17p11.2. To investigate the molecular bases of the SMS phenotype, we constructed BAC/PAC contigs covering the SMS common deletion interval and its syntenic region on mouse chromosome 11. Comparative genome analysis reveals the absence of all three approximately 200-kb SMS-REP low-copy repeats in the mouse and indicates that the evolution of SMS-REPs was accompanied by transposition of adjacent genes. Physical and genetic map comparisons in humans reveal reduced recombination in both sexes. Moreover, by examining the deleted regions in SMS patients with unusual-sized deletions, we refined the minimal Smith-Magenis critical region (SMCR) to an approximately 1.1-Mb genomic interval that is syntenic to an approxiamtely 1.0-Mb region in the mouse. Genes within the SMCR and its mouse syntenic region were identified by homology searches and by gene prediction programs, and their gene structures and expression profiles were characterized. In addition to 12 genes previously mapped, we identified 8 new genes and 10 predicted genes in the SMCR. In the mouse syntenic region of the human SMCR, 16 genes and 6 predicted genes were identified. The SMCR is highly conserved between humans and mice, including 19 genes with the same gene order and orientation. Our findings will facilitate both the identification of gene(s) responsible for the SMS phenotype and the engineering of an SMS mouse model. (+info)

Transcriptional regulation of the stem cell leukemia gene (SCL)--comparative analysis of five vertebrate SCL loci. (4/25)

The stem cell leukemia (SCL) gene encodes a bHLH transcription factor with a pivotal role in hematopoiesis and vasculogenesis and a pattern of expression that is highly conserved between mammals and zebrafish. Here we report the isolation and characterization of the zebrafish SCL locus together with the identification of three neighboring genes, IER5, MAP17, and MUPP1. This region spans 68 kb and comprises the longest zebrafish genomic sequence currently available for comparison with mammalian, chicken, and pufferfish sequences. Our data show conserved synteny between zebrafish and mammalian SCL and MAP17 loci, thus suggesting the likely genomic domain necessary for the conserved pattern of SCL expression. Long-range comparative sequence analysis/phylogenetic footprinting was used to identify noncoding conserved sequences representing candidate transcriptional regulatory elements. The SCL promoter/enhancer, exon 1, and the poly(A) region were highly conserved, but no homology to other known mouse SCL enhancers was detected in the zebrafish sequence. A combined homology/structure analysis of the poly(A) region predicted consistent structural features, suggesting a conserved functional role in mRNA regulation. Analysis of the SCL promoter/enhancer revealed five motifs, which were conserved from zebrafish to mammals, and each of which is essential for the appropriate pattern or level of SCL transcription. (+info)

Prospective screening for subtelomeric rearrangements in children with mental retardation of unknown aetiology: the Amsterdam experience. (5/25)

OBJECTIVE: The frequency of subtelomeric rearrangements in patients with unexplained mental retardation (MR) is uncertain, as most studies have been retrospective and case retrieval may have been biased towards cases more likely to have a chromosome anomaly. To ascertain the frequency of cytogenetic anomalies, including subtelomeric rearrangements, we prospectively screened a consecutive cohort of cases with unexplained MR in an academic tertiary centre. METHODS: Inclusion criteria were: age <18 years at referral, IQ<85, no aetiological diagnosis after complete examination, which included karyotyping with high resolution banding (HRB). RESULTS: In 266 karyotyped children, anomalies were detected in 20 (7.5%, seven numerical, 13 structural); 39 cases were analysed by FISH for specific interstitial microdeletions, and anomalies were found in nine (23%). FISH analyses for subtelomeric microdeletions were performed in 184 children (44% moderate-profound MR, 51% familial MR), and one rearrangement (0.5%) was identified in a non-familial MR female with mild MR (de novo deletion 12q24.33-qter). The number of probable polymorphisms was considerable: 2qter (n=7), Xpter (n=3), and Ypter (n=1). A significantly higher total number of malformations and minor anomalies was present in the cytogenetic anomaly group compared to the group without cytogenetic anomalies. CONCLUSIONS: The total frequency of cytogenetic anomalies in this prospective study was high (1:10), but the frequency of subtelomeric rearrangements was low. The most likely explanations are the high quality of HRB cytogenetic studies and the lack of clinical selection bias. Conventional cytogenetic analyses, combined with targeted microdeletion testing, remain the single most effective way of additional investigation in mentally retarded children, also in a tertiary centre. (+info)

Comparative genomic sequence analysis of the human chromosome 21 Down syndrome critical region. (6/25)

Comprehensive knowledge of the gene content of human chromosome 21 (HSA21) is essential for understanding the etiology of Down syndrome (DS). Here we report the largest comparison of finished mouse and human sequence to date for a 1.35-Mb region of mouse chromosome 16 (MMU16) that corresponds to human chromosome 21q22.2. This includes a portion of the commonly described "DS critical region," thought to contain a gene or genes whose dosage imbalance contributes to a number of phenotypes associated with DS. We used comparative sequence analysis to construct a DNA feature map of this region that includes all known genes, plus 144 conserved sequences > or =100 bp long that show > or =80% identity between mouse and human but do not match known exons. Twenty of these have matches to expressed sequence tag and cDNA databases, indicating that they may be transcribed sequences from chromosome 21. Eight putative CpG islands are found at conserved positions. Models for two human genes, DSCR4 and DSCR8, are not supported by conserved sequence, and close examination indicates that low-level transcripts from these loci are unlikely to encode proteins. Gene prediction programs give different results when used to analyze the well-conserved regions between mouse and human sequences. Our findings have implications for evolution and for modeling the genetic basis of DS in mice. (+info)

Annotation and BAC/PAC localization of nonredundant ESTs from drought-stressed seedlings of an indica rice. (7/25)

To decipher the genes associated with drought stress response and to identify novel genes in rice, we utilized 1540 high-quality expressed sequence tags (ESTs) for functional annotation and mapping to rice genomic sequences. These ESTs were generated earlier by 3'-end single-pass sequencing of 2000 cDNA clones from normalized cDNA libraries constructed form drought-stressed seedlings of an indica rice. A rice UniGene set of 1025 transcripts was constructed from this collection through the BLASTN algorithm. Putative functions of 559 nonredundant ESTs were identified by BLAST similarity search against public databases. Putative functions were assigned at a stringency E value of 10(-6) in BLASTN and BLASTX algorithms. To understand the gene structure and function further, we have utilized the publicly available finished and unfinished rice BAC/PAC (BAC, bacterial artificial chromosome; PAC, P1 artificial chromosome) sequences for similarity search using the BLASTN algorithm. Further, 603 nonredundant ESTs have been mapped to BAC/PAC clones. BAC clones were assigned by a homology of above 95% identity along 90% of EST sequence length in the aligned region. In all, 700 ESTs showed rice EST hits in GenBank. Of the 325 novel ESTs, 128 were localized to BAC clones. In addition, 127 ESTs with identified putative functions but with no homology in IRGSP (International Rice Genome Sequencing Program) BAC/PAC sequences were mapped to the Chinese WGS (whole genome shotgun contigs) draft sequence of the rice genome. Functional annotation uncovered about a hundred candidate ESTs associated with abiotic stress in rice and Arabidopsis that were previously reported based on microarray analysis and other studies. This study is a major effort in identifying genes associated with drought stress response and will serve as a resource to rice geneticists and molecular biologists. (+info)

Evidence for a fast, intrachromosomal conversion mechanism from mapping of nucleotide variants within a homogeneous alpha-satellite DNA array. (8/25)

Assuming that patterns of sequence variants within highly homogeneous centromeric tandem repeat arrays can tell us which molecular turnover mechanisms are presently at work, we analyzed the alpha-satellite tandem repeat array DXZ1 of one human X chromosome. Here we present accurate snapshots from this dark matter of the genome. We demonstrate stable and representative cloning of the array in a P1 artificial chromosome (PAC) library, use samples of higher-order repeats subcloned from five unmapped PACs (120-160 kb) to identify common variants, and show that such variants are presently in a fixed transition state. To characterize patterns of variant spread throughout homogeneous array segments, we use a novel partial restriction and pulsed-field gel electrophoresis mapping approach. We find an older large-scale (35-50 kb) duplication event supporting the evolutionarily important unequal crossing-over hypothesis, but generally find independent variant occurrence and a paucity of potential de novo mutations within segments of highest homogeneity (99.1%-99.3%). Within such segments, a highly nonrandom variant clustering within adjacent higher-order repeats was found in the absence of haplotypic repeats. Such variant clusters are hardly explained by interchromosomal, fixation-driving mechanisms and likely reflect a fast, localized, intrachromosomal sequence conversion mechanism. (+info)

P1 artificial chromosomes (PACs) have features of both P1 vectors and Bacterial Artificial Chromosomes (BACs). Similar to P1 ... Bacteriophage P1 vectors can hold inserts 70 - 100kb in size. They begin as linear DNA molecules packaged into bacteriophage P1 ... Yeast artificial chromosomes (YACs) are linear DNA molecules containing the necessary features of an authentic yeast chromosome ... Unlike P1 vectors, they do not need to be packaged into bacteriophage particles for transduction. Instead they are introduced ...

https://en.wikipedia.org/wiki/Genomic_library

... chromosomes, artificial, human MeSH A11.284.187.178.195 - chromosomes, artificial, p1 bacteriophage MeSH A11.284.187.178.200 - ... chromosomes, artificial MeSH A11.284.187.178.170 - chromosomes, artificial, bacterial MeSH A11.284.187.178.190 - chromosomes, ... chromosomes, artificial, yeast MeSH A11.284.187.190 - chromosomes, bacterial MeSH A11.284.187.190.170 - chromosomes, artificial ... chromosomes, artificial, yeast MeSH A11.284.187.520 - chromosomes, mammalian MeSH A11.284.187.520.190 - chromosomes, artificial ...

https://en.wikipedia.org/wiki/List_of_MeSH_codes_(A11)

A P1-derived artificial chromosome is a DNA construct that was derived from the DNA of P1 bacteriophage. It can carry large ... Online Medical Dictionary P1-derived artificial chromosome P1-derived artificial chromosome (PAC) definition v t e v t e c. ... Bacterial artificial chromosome Human artificial chromosome Yeast artificial chromosome Yarmolinsky M, Hoess R (November 2015 ... Sternberg N (January 1990). "Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as ...

https://en.wikipedia.org/wiki/P1-derived_artificial_chromosome

A similar cloning vector called a PAC has also been produced from the DNA of P1 bacteriophage. ... Yeast artificial chromosome. References[edit]. *^ O'Connor M, Peifer M, Bender W (2018). "Construction of large DNA segments in ... A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for ... The bacterial artificial chromosome's usual insert size is 150-350 kbp.[4] ...

https://en.wikipedia.org/wiki/Bacterial_artificial_chromosome

BACs are based on F plasmid, another artificial chromosome called the PAC is based on the P1 phage. Yeast artificial chromosome ... The bacteriophages used for cloning are the λ phage and M13 phage. There is an upper limit on the amount of DNA that can be ... Human artificial chromosome may be potentially useful as a gene transfer vectors for gene delivery into human cells, and a tool ... Insert size of up to 350 kb can be cloned in bacterial artificial chromosome (BAC). BACs are maintained in E. coli with a copy ...

https://en.wikipedia.org/wiki/Cloning_vector

P1 can also be used to create the P1-derived artificial chromosome cloning vector which can carry relatively large fragments of ... "Bacteriophage P1", in Richard Calendar (ed.), The Bacteriophages, Oxford University Press, p. 350, ISBN 0195148509 Viralzone: ... "DNA Inversion Regions Min of Plasmid p15B and Cin of Bacteriophage P1: Evolution of Bacteriophage Tail Fiber Genes". Journal of ... The genome of P1 encodes 112 proteins and 5 untranslated genes and is this about twice the size of bacteriophage lambda. The ...

https://en.wikipedia.org/wiki/P1_phage

In engineering large constructs of >100 kb, such as the Bacterial Artificial Chromosomes (BACs), or chromosomes, recombineering ... As proteins homologous to Beta and RecT are found in many bacteria and bacteriophages (>100 as of February 2010), ... recombineering and P1 transduction in Escherichia coli". Nucleic Acids Research. 41 (22): e204. doi:10.1093/nar/gkt1075. PMC ... and for modifying DNA of any source often contained on a bacterial artificial chromosome (BAC), among other applications. ...

https://en.wikipedia.org/wiki/Recombineering

... usually occurs as linear chromosomes in eukaryotes, and circular chromosomes in prokaryotes. The set of chromosomes in a ... Artificial bases. Main article: Nucleic acid analogue. Several artificial nucleobases have been synthesized, and successfully ... bacteriophages) is to avoid the restriction enzymes present in bacteria. This enzyme system acts at least in part as a ... such as in chromosome 1. Chromosome 1 is the largest human chromosome with approximately 220 million base pairs, and would be ...

https://en.wikipedia.org/wiki/Dna

4.4 Bacteriophage P1 derived vector. 4.5 P1 derived artificial chromosome (PAC). 4.6 Bacterial artificial chromosomes (BAC) ...

https://www.elsevier.com/books/concepts-and-techniques-in-genomics-and-proteomics/saraswathy/978-1-907568-10-7

Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as large as 100 kilobases pairs ... such as P1 bacteriophage (=-=Sternberg, 1990-=-), bacterial artificial chromosomes (BACs) (Shizuya et al., 1992) and P1 ... The genome of bacteriophage P1 by Malgorzata B. Lobocka, Debra J. Rose, Guy Plunkett Iii, Marek Rusin, Arkadiusz Samojedny, ... Bacteriophage P1 cloning system for the isolation, amplification and recovery of DNA fragments as large as 100 kilobases pairs ...

http://citeseerx.ist.psu.edu/showciting?cid=1091564

One of these recombination systems that utilize specific recognition sites is the Cre/loxP system of bacteriophage P1. It ... A. Domi and B. Moss, "Engineering of a vaccinia virus bacterial artificial chromosome in Escherichia coli by bacteriophage λ- ... Viral Bacterial Artificial Chromosomes: Generation, Mutagenesis, and Removal of Mini-F Sequences. B. Karsten Tischer and ... A. Domi and B. Moss, "Cloning the vaccinia virus genome as a bacterial artificial chromosome in Escherichia coli and recovery ...

https://www.hindawi.com/journals/bmri/2012/472537/

A similar cloning vector called a PAC has also been produced from the DNA of P1 bacteriophage. ... Yeast artificial chromosome. References[edit]. *^ OConnor M, Peifer M, Bender W (2018). "Construction of large DNA segments in ... A bacterial artificial chromosome (BAC) is a DNA construct, based on a functional fertility plasmid (or F-plasmid), used for ... The bacterial artificial chromosomes usual insert size is 150-350 kbp.[4] ...

https://en.wikipedia.org/wiki/Bacterial_artificial_chromosome

A P1-derived artificial chromosome is a DNA construct that was derived from the DNA of P1 bacteriophage. It can carry large ... Yeast artificial chromosome. References[edit]. *^ Yarmolinsky M, Hoess R (November 2015). "The Legacy of Nat Sternberg: The ... Retrieved from "https://en.wikipedia.org/w/index.php?title=P1-derived_artificial_chromosome&oldid=903800665" ... "Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs" ...

https://en.wikipedia.org/wiki/P1-derived_artificial_chromosome

... bacteriophage derived artificial chromosomes) or PACs (P1 derived artificial chromosomes) or combinations thereof. Artificial ... bacteriophage-derived artificial chromosome (BBPAC), cosmid or P1 derived artificial chromosome (PAC), that can be transfected ... Artificial chromosomes include, without limitation, BACs (bacterial artificial chromosomes), YACs (yeast artificial chromosomes ... bacteriophage-derived artificial chromosome (BBPAC), cosmid or P1 derived artificial chromosome (PAC). ...

http://www.google.com/patents/US7507873?dq=5920316

... such as P1 bacteriophage (Sternberg, 1990), bacterial artificial chromosomes (BACs) (Shizuya et al., 1992) and P1 artificial ... Cre (causes recombination of the bacteriophage P1 genome) recognizes minimal loxP [locus of crossing-over (X) in P1] RSs of 34 ... Gong, S., Yang, X. W., Li, C. and Heintz, N. (2002). Highly efficient modification of bacterial artificial chromosomes (BACs) ... Sternberg, N. (1990). Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large ...

http://dev.biologists.org/content/134/20/3571

derived from bacteriophage P1 vector. *15-16kb. *positive selection for inserts via sacB the levansucrase gene which converts ... Bacterial artificial chromosomes or BACS are circular DNA molecules which contain a replicon that is based on the F factor. ... A modular, positive selection bacterial artificial chromosome vector with multiple cloning sites. Genomics. 1999 Jun 15;58(3): ... Bacteriophage lambda cos site for fosmid packaging or lambda terminase cleavage. *Phage T7 promoter for in vitro transcription ...

https://openwetware.org/wiki/Bacterial_artificial_chromosomes

Sauer B, Henderson N. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. ... Domi A, Moss B. Engineering of a vaccinia virus bacterial artificial chromosome in Escherichia coli by bacteriophage λ-based ... Bacterial Artificial Chromosome Mutagenesis Using Recombineering. Kumaran Narayanan 1, 2 * and Qingwen Chen 2 ... Construction of human artificial chromosome vectors by recombineering. Gene. 2005;351:29-38. [PubMed] ...

http://pubmedcentralcanada.ca/pmcc/articles/PMC3005948/

... bacterial artificial chromosome (BAC), or P1-derived artificial chromosome (PAC), bacteriophages such as lambda phage or M13 ... Examples of vectors include plasmids, phagemids, cosmids, artificial chromosomes such as yeast artificial chromosome (YAC), ... Genes Chromosomes Cancer 27:95-103.. *34. Saffran, D. C., et al. 2001. Anti-PSCA mAbs inhibit tumor growth and metastasis ... "Isolated" as used herein does not exclude artificial or synthetic mixtures with other compounds or materials, or the presence ...

http://www.google.co.uk/patents/US8088908

PAC The artificial chromosome vector derived from the temperate bacteriophage, P1, used for cloning 100- to 200-kb DNA ... bacterial artificial chromosome (BAC) Artificial chromosome vector derived from bacteria used for cloning relatively large DNA ... ring chromosome A structurally abnormal chromosome in which the end of each chromosome arm has been deleted and the broken arms ... BAC See bacterial artificial chromosome.. backcross A genetic crossing of a heterozygous organism and one of its homozygous ...

https://www.kumc.edu/gec/gloss.html

... bacterial artificial chromosomes, and a P1 bacteriophage vectors, also described as P1-derived artificial chromosomes.68 69 70 ... Construction and characterization of a 10-fold genome equivalent rat P1-derived artificial chromosome library. Genomics. 1998; ... 71 If the markers are close on the chromosome, then the flanking yeast artificial chromosomes clones will overlap, as judged by ... Construction of a large-insert yeast artificial chromosome library of the rat genome. Mamm Genome. 1997;8:284. ...

http://hyper.ahajournals.org/content/35/1/164

https://www.freepatentsonline.com/y2008/0152623.html

faagi P1 kunstlik kromosoom (ingl. Bacteriophage P1 artificial chromosome). Faagi P1 plasmiidvektori katkilõikamisel tekib ...

http://geneetika.ee/lexicon/filter:f/

Yeast Artificial Chromosomes Medicine & Life Sciences * Cosmids Medicine & Life Sciences * Bacteriophage P1 Medicine & Life ... The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. The majority of the ... The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. The majority of the ... The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. The majority of the ...

https://mayoclinic.pure.elsevier.com/en/publications/a-yac-p1-and-cosmid-based-physical-map-of-the-brca1-region-on-chr

1994) A new bacteriophage P1‐derived vector for the propagation of large human DNA fragments. Nature Genetics 6(1): 84-89. ... Burke DT, Carle GF and Olson MV (1987) Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome ... Sternberg N (1990) Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as ... Sauer B and Henderson N (1988) Site‐specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. ...

http://www.els.net/WileyCDA/ElsArticle/refId-a0005344.html

P. A. Ioannou, C. T. Amemiya, J. Garnes et al., "A new bacteriophage P1-derived vector for the propagation of large human DNA ... Bacterial Artificial Chromosome (BAC) libraries have been used extensively for constructing physical maps of the human genome ... J. P. P. Muyrers, Y. Zhang, G. Testa, and A. F. Stewart, "Rapid modification of bacterial artificial chromosomes by ET- ... K. Osoegawa, P. Y. Woon, B. Zhao et al., "An improved approach for construction of bacterial artificial chromosome libraries," ...

https://www.hindawi.com/journals/bmri/2011/560124/

We reported previously that ~80-kilobase pair (kb) P1 bacteriophage clones spanning either the human or mouse apoB gene (clones ... To test this possibility, transgenic mice were generated with 145- and 207-kb bacterial artificial chromosomes (BACs) that ... Human Apolipoprotein B Transgenic Mice Generated with 207- and 145-Kilobase Pair Bacterial Artificial Chromosomes. Evidence ... and 145-Kilobase Pair Bacterial Artificial Chromosomes. Evidence that a distant 5-element confers appropriate transgene ...

https://authors.library.caltech.edu/11003/

Yeast Artificial Chromosomes (YACs). • Bacterial artificial chromosomes (BACs). • Bacteriophage P1 artificial chromosomes. ( ... Bacteriophage P1 artificial chromosomes. (PACs) have been constructed from. bacteriophage P1 chromosomes.. • BACs and PACs ... Bacteriophage Vectors. • Most bacteriophage cloning vectors have. been constructed from the phage λ. chromosome.. • The central ... Yeast Artificial Chromosomes (YACs). • Genetically engineered yeast minichromosomes.. • Accept foreign DNA inserts of 200-500 ...

https://fr.scribd.com/presentation/28382685/Genetic-Engineering-UNIT-I-A

P1 Bacteriophage Artificial Chromosomes * Radiation Hybrid Mapping * Chromosomes 37 Scopus citations *‹ Previous ...

https://augusta.pure.elsevier.com/en/publications/?page=8&ordering=type&descending=false&showAdvanced=false&allConcepts=true&inferConcepts=true&originalSearch=&improvedLayoutOrganisationUuid=&format=

... human ELKS gene from within a 700-kb genomic region represented by overlapping bacteriophage P1-derived artificial chromosome ( ... PAC) and bacterial artificial chromosome (BAC) clones, and localized it to chromosomal band 12p13.3 by fluorescence in situ ... Genes Chromosomes Cancer. 2002; 35(1):30-7 [PubMed] Related Publications A novel gene, ELKS, whose 5 portion was fused to the ... Genes Chromosomes Cancer. 1999; 25(2):97-103 [PubMed] Related Publications In papillary thyroid carcinomas, the genes for ...

http://www.cancerindex.org/geneweb/ELKS.htm

Chromosomes, Artificial, Bacterial/genetics , Chromosomes, Artificial, P1 Bacteriophage/genetics , Disasters , Expressed ... bacterial artificial chromosome; PAC, P1 artificial chromosome) sequences for similarity search using the BLASTN algorithm. ...

https://search.bvsalud.org/gim/?lang=en&q=mh:Chromosomes,+Artificial,+P1+Bacteriophage/genetics

P1 Bacteriophage Artificial Chromosomes Medicine & Life Sciences * Radiation Hybrid Mapping Medicine & Life Sciences ... we have constructed a 2-Mb bacterial and P1-derived artificial chromosome (BAC-PAC) contig for the EGFR region and have applied ... we have constructed a 2-Mb bacterial and P1-derived artificial chromosome (BAC-PAC) contig for the EGFR region and have applied ... we have constructed a 2-Mb bacterial and P1-derived artificial chromosome (BAC-PAC) contig for the EGFR region and have applied ...

https://indiana.pure.elsevier.com/en/publications/a-chromosomal-region-7p112-transcript-map-its-development-and-app

Chromosomes, Artificial, Bacterial/genetics. *Chromosomes, Artificial, P1 Bacteriophage/genetics. *Chromosomes, Artificial, ... coli homologous recombination systems have recently been developed that enable genomic DNA in bacterial artificial chromosomes ... This new form of chromosome engineering, termed recombinogenic engineering or recombineering, is efficient and greatly ...

https://www.ncbi.nlm.nih.gov/pubmed/11584293?dopt=Abstract

... a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, ... Yeast artificial chromosome (YAC) clones covering the entire genome were used to subdivide the libraries, and hybridization of ... High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe Cell. 1993 Apr 9;73(1):109-20. doi: ... Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both ...

https://pubmed.ncbi.nlm.nih.gov/8462094/?dopt=Abstract

P1-derived Artificial Chromosome. DNA constructs that are derived from the DNA of P1 bacteriophage. They can carry large ... Yeast Artificial Chromosomes are extremely large segments of DNA from another species spliced into DNA of yeast. YACs are used ... Bacterial Artificial Chromosome (BAC) is a DNA construct, based on a fertility plasmid, used for transforming and cloning in ... A virus used to construct vectors that are able to stably integrate any gene cassette into the host cell chromosome. This ...

http://www.wikimd.org/wiki/Biotechnology

An ordered yeast artificial chromosome library covering over half of rice chromosome 6. ... a P1 plasmid replicon to stably maintain that DNA in E. coli at one copy per cell chromosome, and a lac promoter-regulated P1 ... Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs ... Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs ...

https://www.pnas.org/content/87/1/103?ijkey=f42ccf2866fdec205904b477ef13f0a9ccaba0cc&keytype2=tf_ipsecsha

Chromosome. UN. Molecular Note. A mouse bacterial artificial chromosome (BAC) containing the entire Npas1 gene, was modified by ... cre, cre recombinase, bacteriophage P1. Expressed Gene. RFP, Red Fluorescent Protein, coral. ... A mouse bacterial artificial chromosome (BAC) containing the entire Npas1 gene, was modified by inserting Cre-2A (cleavable ...

https://www.jax.org/strain/027718

however, this problem could also be overcome by the use of single or low-copy vectors, such as bacterial artificial chromosomes (BACs). (hindawi.com)
Bacterial artificial chromosomes or BACS are circular DNA molecules which contain a replicon that is based on the F factor. (openwetware.org)
Bacterial artificial chromosomes, or BACs, are fertility- (F-) factor-based plasmid vectors that replicate stably in low copy number [ 2 , 3 ]. (pubmedcentralcanada.ca)
Human Apolipoprotein B Transgenic Mice Generated with 207- and 145-Kilobase Pair Bacterial Artificial Chromosomes. (caltech.edu)
To test this possibility, transgenic mice were generated with 145- and 207-kb bacterial artificial chromosomes (BACs) that contained the human apoB gene and more extensive 5'- and 3'-flanking sequences. (caltech.edu)
Highly efficient phage-based Escherichia coli homologous recombination systems have recently been developed that enable genomic DNA in bacterial artificial chromosomes to be modified and subcloned, without the need for restriction enzymes or DNA ligases. (nih.gov)
Bacterial artificial chromosomes (BACs) and P1 artificial chromosomes (PACs) are widely used to investigate the functions of genes and genomes in mammalian cells in vitro and in vivo. (semanticscholar.org)
Bacterial artificial chromosomes (BACs) and P1-based artificial chromosomes (PACs) have proved excellent tools for the human genome sequencing projects. (ox.ac.uk)
Yang Y, Seed B (2003) Site-specific gene targeting in mouse embryonic stem cells with intact bacterial artificial chromosomes. (springer.com)
By sequencing 8452 bacterial artificial chromosomes in pools, we assembled a sequence of 774 megabases carrying 5326 protein-coding genes, 1938 pseudogenes, and 85% of transposable elements. (jove.com)
Plasmids that utilize different combinations of double-counter selective markers have been used for diverse applications, including the search for extremely rare suppressor mutations of essential Escherichia coli genes, and to improve the efficiency of allelic exchange on bacterial artificial chromosomes (BACs). (asmscience.org)

BACs can also be utilized to detect genes or large sequences of interest and then used to map them onto the human chromosome using BAC arrays . (wikipedia.org)
Genomic DNA libraries contain large fragments of DNA in either bacteriophages or bacterial or P1-derived artificial chromosomes (BACs and. (thedentistinworthington.com)

Gene expression from bacterial artificial chromosome (BAC) clones has been demonstrated to facilitate physiologically relevant levels compared to viral and nonviral cDNA vectors. (pubmedcentralcanada.ca)
The widespread use of yeast artificial chromosomes as cloning vectors, has created a demand for reliable method to recover intact chromosomal DNA from preparative pulsed field gels. (thefreedictionary.com)
Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors" Science, 236:806-812 (1987). (patentgenius.com)

One of these is recombineering (recombination-mediated genetic engineering [ 14 ]), which was adapted from bacteriophage where the recombination genes were carefully delineated and moved to mobile plasmid systems that were transferable to host E. coli strains. (pubmedcentralcanada.ca)
We have developed a new approach to screen bacterial artificial chromosome (BAC) libraries by recombination selection. (hindawi.com)
Sternberg N, Hamilton D (1981) Bacteriophage P1 site-specific recombination. (springer.com)
The distribution of structural and functional features along the chromosome revealed partitioning correlated with meiotic recombination. (jove.com)
A recombination system has been developed for efficient chromosome engineering in Escherichia coli by using electroporated linear DNA. (pnas.org)
Murphy ( 9 ) reported the highest frequencies of recombination with linear DNA containing long homologies by transforming in the presence of the bacteriophage λ recombination functions (Exo and Beta) in a bacterial recBCD mutant background. (pnas.org)
An impressive application of Flp in eukaryotes has been the creation of mosaic flies in Drosophila by site-specific recombination between homologous chromosomes. (asmscience.org)
Finally, after a recombination event, the new DNA is integrated into the chromosome. (asm.org)
Cre can be expressed in Tetrahymena and localizes to the macronucleus where it induces precise recombination at two loxP sequences in direct orientation in the Tetrahymena macronuclear chromosome. (biomedcentral.com)
Cre is a recombinase from the bacteriophage P1 that mediates intramolecular and intermolecular site-specific recombination between two loxP sites [ 11 ]. (biomedcentral.com)
A new vector for recombination-based cloning of large DNA fragments from yeast artificial chromosomes" Nucl. (patentgenius.com)
The temperature-sensitive vector also carries a distinct antibiotic-resistance marker (abr2) and may also include a counterselection marker such sac B. Integration of the vector into the host chromosome occurs at the nonpermissive temperature by homologous recombination (crossover event at position A). The configuration of the integrated plasmid is shown in the middle, showing the duplication of the targeted sequences. (asmscience.org)
cre, the site-specific recombinase from bacteriophage p1, catalyzes a recombination reaction between specific dna sequences designated as lox sites. (liverpool.ac.uk)
the transposon contains the ura3 gene, the teta gene, a truncated lacz, and phage p1 loxp recombination sites at either end. (liverpool.ac.uk)
Site-specific recombination by the bacteriophage P1 lox-Cre system. (geneticsmr.com)
Sauer B, Henderson N. Site-specific DNA recombination in mammalian cells by the Cre recombinase of bacteriophage P1. (biomedcentral.com)
It usually (but not always) based on λRed- or RecET-mediated recombination between bacterial chromosome and amplified DNA fragment carrying the removable selective marker, in which PCR primers provide the rather short homology to the targeted sequence. (biomedcentral.com)
The integrated marker could be excised out of the chromosome by site-specific recombination. (biomedcentral.com)
By using cloned fragments of chromosomes as so-called "guides" [ 4 ] it is possible to integrate the cassette by general homologous recombination. (biomedcentral.com)

The C57BL/6 mouse bacterial artificial chromosome (BAC) 148M1 containing the entire Ucp1 gene, and other genes, was modified by inserting sequence encoding Cre recombinase, into the translation initiation site into exon 1 of the Ucp1 gene. (jax.org)
movement of cosN and loxP sites(cloned in by bacteriophage l terminase and P1 Cre recombinase, respectively) permits linearization of the plasmid for convenient restriction mapping.There is a chloramphenicol resistance gene for negative selection of non-transformed bacteria. (blogspot.com)
Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs. (mmrrc.org)
in the presence of the phage p1 cyclization recombinase cre, the transposon can delete the ura3, teta, and lacz genes between the two loxp sites. (liverpool.ac.uk)

The generation of a complete physical map of the human genome should be achieved by the use of large segments of DNA contained in yeast artificial chromosomes (18), P1 clones =-=(34)-=-, and cosmid or phage contigs (32, 33). (psu.edu)
This approach has mostly been followed using bacteriophage lambda libraries amplified on a host containing a "probe" plasmid and then tested in a restrictive E. coli strain for selective growth of recombinant lambda clones with an integrated plasmid [ 2 - 5 ]. (hindawi.com)
We reported previously that ~80-kilobase pair (kb) P1 bacteriophage clones spanning either the human or mouse apoB gene (clones p158 and p649, respectively) confer apoB expression in the liver of transgenic mice, but not in the intestine. (caltech.edu)
Gridded on high density filters, a P1 genomic library of 17-fold coverage and a cosmid library of 8 genome equivalents, both made from S. pombe strain 972h-, were ordered by hybridizing genetic markers and individual clones from the two libraries. (nih.gov)
Yeast artificial chromosome (YAC) clones covering the entire genome were used to subdivide the libraries, and hybridization of short oligonucleotides and DNA pools made from randomly selected cosmids provided further mapping information. (nih.gov)
A DNA contig of this region spanning 2,400 kb was constructed from large-capacity yeast artificial chromosomes and P1 bacteriophage clones (76). (thefreedictionary.com)
We also describe a method for the bulk testing of the hybridization characteristics of chromosome-specific clones spotted on microarrays by use of DNA amplified from flow-sorted chromosomes. (ox.ac.uk)
A total of 26 genomic and 21 cDNA markers derived from Arabidopsis yeast artificial and bacterial artificial chromosome clones were used to analyze this region in the two genomes. (plantcell.org)
The physical maps that we derived by using these markers as well as markers isolated from bacteriophage clones spanning the S 8 haplotype revealed a high degree of synteny at the submegabase scale between the two homeologous regions. (plantcell.org)
Large insert recombinant DNA clones such as bacterial artificial chromosome (BAC) or P1/PAC clones have established themselves in recent years as preferred starting material for probe preparations due to their low rates of chimerism and ease of use. (biomedcentral.com)

candidate gene approach Strategy to identify disease-associated genes based on finding candidate genes in a chromosome region in which a disorder is mapped. (kumc.edu)
A familial early-onset breast cancer gene (BRCA1) has been localized to chromosome 17q21. (elsevier.com)
To characterize this region and to aid in the identification of the BRCA1 gene, a physical map of a region of 1.0-1.5 Mb between the EDH17B1 and the PPY loci on chromosome 17q21 was generated. (elsevier.com)
A mouse bacterial artificial chromosome (BAC) containing the entire Npas1 gene, was modified by inserting Cre-2A (cleavable fusion peptide sequence)-tdTomato and polyadenylation sequence from bovine growth hormone downstream of the ATG translation initiation codon, and removing Npas1 coding sequence. (jax.org)
In practice, localization of the gene to a chromosome or genomic region does not necessarily enable one to isolate or amplify the relevant genomic sequence. (f-mx.ru)
Assignment of the SLC25A12 gene coding for the human calcium-binding mitochondrial solute carrier protein aralar to human chromosome 2q24. (sickkids.ca)
Starting with markers flanking the self-incompatibility genes in Brassica , we identified the homeologous region in Arabidopsis as a previously uncharacterized segment of chromosome 1 in the immediate vicinity of the ethylene response gene ETR1 . (plantcell.org)
We describe here a P1-like bacteriophage, RCS47, carrying a blaSHV-2 gene, isolated from a clinical strain of Escherichia coli from phylogroup B1, and we report the prevalence of P1-like prophages in natural E. coli isolates. (jove.com)
The reference P1 prophage plasmid replication gene belonged to the IncY incompatibility group, whereas that of RCS47 was from an unknown group. (jove.com)
A transgenic animal is an animal which has been genetically modified by the stable incorporation, by using artificial gene transfer, of exogenous DNA into its genome, in order to introduce or delete specific characteristics of the phenotype. (jhsph.edu)
Gene disruptions and modifications of both the bacterial chromosome and bacterial plasmids are possible. (pnas.org)
In rec + backgrounds, gene replacement on the chromosome has been accomplished by integration and excision of episomes carrying bacterial homology ( 13 , 14 ). (pnas.org)
We have generated a novel mouse model where the endogenous Arc/Arg3.1 gene is tagged in its 3′ untranslated region with stem-loops that bind a bacteriophage PP7 coat protein (PCP), allowing visualization of individual mRNAs in real time. (sciencemag.org)
the c1 repressor gene of bacteriophage p1 and the temperature-sensitive mutants p1c1.100 and p1c1.162 was cloned into an expression vector and the repressor proteins were overproduced. (liverpool.ac.uk)
Sternberg N, Sauer B, Hoess R, Abremski K. Bacteriophage P1 cre gene and its regulatory region. (biomedcentral.com)
Two examples demonstrate the application of this technique: mapping of a gene-specific ~6 kb plasmid onto an unusually small, ~55 kb circular P1 molecule and the determination of the extent of overlap between P1 molecules homologous to the human NF-κB2 locus. (biomedcentral.com)

[4] A similar cloning vector called a PAC has also been produced from the DNA of P1 bacteriophage. (wikipedia.org)
P1 was developed as a cloning vector by Nat Sternberg and colleagues in the 1990s. (wikipedia.org)
5. The transgenic avian of claim 4 , wherein the vector is selected from the group consisting of a plasmid, a viral vector, and an artificial chromosome. (google.com)
bacterial artificial chromosome (BAC) Artificial chromosome vector derived from bacteria used for cloning relatively large DNA fragments. (kumc.edu)
Presumably, the combined size of the latter fragment and the vector DNA (13 kbp) exceeds the headful capacity of P1. (pnas.org)
5. carrier or expression cassette, it comprises nucleotide sequence according to claim 1, and wherein said carrier or expression cassette are included in virus vector, plasmid, phage, phagemid, clay, artificial chromosome. (rpxcorp.com)
6. carrier or expression cassette, it comprises nucleotide sequence according to claim 1, and wherein said carrier or expression cassette are adenovirus carrier, retroviral vector, gland relevant viral vector, bacterial artificial chromosome, bacteriophage P1 derivative vector, yeast artificial chromosome or artificial mammalian chromosome. (rpxcorp.com)
Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. (sickkids.ca)
7. A vector according to claim 2, wherein said vector is selected from the group consisting of a plasmid, virus, and bacteriophage. (patentgenius.com)
in vitro packaging of a lambda dam vector containing ecori dna fragments of escherichia coli and phage p1. (liverpool.ac.uk)

X-chromosome inactivation ensures equal expression of mammalian male and female X-linked genes by transcriptionally silencing one X chromosome in each female cell. (ubc.ca)
the use of yeast artificial chromosomes permits the cloning of large genes with their flanking regulatory sequences. (thefreedictionary.com)
In 1996 Resnick, with NIEHS colleagues Vladimir Larionov and Natalya Kouprina, perfected a method using yeast artificial chromosomes , or YACs, for the specific isolation of entire human genes. (thefreedictionary.com)
For example, the rRNA genes from different chromosomes of species A might be homo- geneous in sequence structure and those from spe- cies B might also be homogeneous. (damasgate.com)
Meloche S, Gopalbhai K, Beatty BG, Scherer SW, Pellerin J. Chromosome mapping of the human genes encoding the MAP kinase kinase MEK1 (MAP2K1) to 15q21 and MEK2 (MAP2K2) to 7q32. (sickkids.ca)
One emerging method involves inserting artificial genes into bacterial genomes and examining how the genome and its new genes adapt to each other. (springer.com)

Bacterial Artificial Chromosome (BAC) libraries have been used extensively for constructing physical maps of the human genome as well as for whole-genome sequencing projects. (hindawi.com)
The temperate bacteriophage WO is the only known mobile genetic element that transforms the genome of Wolbachia and thus is an ideal object for studying the three-way interactions among viruses, bacteria, and eukaryotes ( 6 , 7 ). (asm.org)
Construction and characterization of a 10-fold genome equivalent rat P1 derived artificial chromosome library. (bacpacresources.org)

A bacterial artificial chromosome ( BAC ) is a DNA construct , based on a functional fertility plasmid (or F-plasmid ), used for transforming and cloning in bacteria , usually E. coli . (wikipedia.org)
Currently, her research focus is on chromosome folding in bacteria. (whsmith.co.uk)
DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. (uchicago.edu)
Bacteriophages are viruses that infect bacteria. (golden.com)
P1 bacteriophages lysogenize bacteria as independent plasmid-like elements. (jove.com)
Bacteria have few structural or developmental features that can be observed easily, but they have a vast array of biochemical capabilities and patterns of susceptibility to antimicrobial agents or bacteriophages. (nih.gov)
Chemically modified purine and pyrimidine bases are found in some bacteria and bacteriophages. (nih.gov)
This mechanism is found in gram-positive bacteria like ''Staphylococcus aureus'' and ''Streptomyces lividans'' as well as many bacteriophages. (openwetware.org)
2. BACTERIOPHAGE  Virus that infect bacteria is known as bacteriophage. (slideshare.net)
The chromosomes of many bacteria have been found to carry toxin-antitoxin systems that are homologous to these extrachromosomal addiction modules ( 3 , 10 , 11 , 20 , 29 , 30 , 31 , 33 ). (asm.org)
Temperate bacteriophage WO is a model system for studying tripartite interactions among viruses, bacteria, and eukaryotes, especially investigations of the genomic stability of obligate intracellular bacteria. (asm.org)

Cosmid End-sequence profiling Fosmid Human artificial chromosome Yeast artificial chromosome O'Connor M, Peifer M, Bender W (June 1989). (wikipedia.org)
Bacterial artificial chromosome Human artificial chromosome Yeast artificial chromosome Yarmolinsky M, Hoess R (November 2015). (wikipedia.org)
The physical map is composed of a yeast artificial chromosome (YAC) and P1 phage contig with one gap. (elsevier.com)
Although investigators have made yeast artificial chromosomes for more than a decade (SN: 6/5/93, p. (thefreedictionary.com)
Hadano S, Nichol K, Brinkman RR, Nasir J, Martindale D, Koop BF, Nicholson DW, Scherer SW, Ikeda JE, Hayden MR. A yeast artificial chromosome-based physical map of the juvenile amyotrophic lateral sclerosis (ALS2) critical region on human chromosome 2q33-q34. (sickkids.ca)

Sternberg N, Austin S, Hamilton D, Yarmolinsky M. Analysis of bacteriophage P1 immunity by using lambda-P1 recombinants constructed in vitro. (biomedcentral.com)

The development of a bacteriophage P1 cloning system capable of accepting DNA fragments as large as 100 kilobase pairs (kbp) is described. (pnas.org)

These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms. (sickkids.ca)
Chromosome-based techniques, such as comparative genomic hybridization (CGH) and fluorescent in situ hybridization (FISH) facilitate efforts to cytogenetically localize genomic regions that are altered in tumor cells. (justia.com)

Many bacterial chromosomes and low-copy plasmids, such as the plasmids P1 and F, employ a three-component system to partition replicated genomes: a partition site on the DNA target, typically called parS, a partition site binding protein, typically called ParB, and a Walker-type ATPase, typically called ParA, which also binds non-specific DNA. (nih.gov)
Extrachromosomal genetic elements such as plasmids and bacteriophages are nonessential replicons which often determine resistance to antimicrobial agents, production of virulence factors, or other functions. (nih.gov)
This system will be especially useful for the engineering of large bacterial plasmids such as those from bacterial artificial chromosome libraries. (pnas.org)
Theta is the most common form of DNA replication, including most plasmids as well as chromosomes. (openwetware.org)

PAC, P1 artificial chromosome) sequences for similarity search using the BLASTN algorithm. (bvsalud.org)

free Mecánica and clear and Mammalian, will remember a P1-aha development, where P1 is the attP)-LB M-step exchange and aha is the different interest modelling source. (scoutconnection.com)

In 1972, Paul Berg and colleagues made the first artificial recombinant DNA molecule. (f-mx.ru)
Here, we describe the new scheme of insertion of the foreign DNA for step-by-step construction of plasmid-less marker-less recombinant E. coli strains with chromosome structure designed in advance. (biomedcentral.com)
This new approach allows us to detail the design of future recombinant marker-less strains, carrying, in particular, rather large artificial insertions that could be difficult to introduce by usually used PCR-based Recombineering procedure. (biomedcentral.com)

Homoeologous relationship of rice, wheat and maize chromosomes" Molecular and General Genetics, 241:483-90 (1993). (patentgenius.com)

The Bacterial Dna Molecule.The Structure Of DNA And RNA.Deoxyribonucleosides And Deoxyribonucleotides.DNA Is Only Polymerized 5' To 3'.Double-Stranded Dna.Supercoiling Double-Stranded Dna.Replication Of The Escherichia Coli Chromosome.Constraints That Influence Dna Replication.The Replication Machinery.Dna Polymerases.Dnag Primase.Replication Of Both Strands.Theta Mode Replication.Minimizing Mistakes In Dna Replication.The Dna Replication Machinery As Molecular Tools.Summary.3. (whsmith.co.uk)
lactamase (ESBL)-producing and non-ESBL-producing strains (P = 0.69), but this prevalence was lower in phylogroup B2 than in the other phylogroups (P = 0.008), suggesting epistatic interactions between P1 family phages and the genetic background of E. coli strains. (jove.com)

bivalent A pair of homologous chromosomes in association as seen at metaphase of the first meiotic division. (kumc.edu)
A designation of the specific alleles present on the two homologous chromosomes for a given locus . (metacyc.org)

The bacterial chromosome is a circular molecule of DNA that functions as a self-replicating genetic element (replicon). (nih.gov)

We found that 70% of the sequence of RCS47, a 115-kb circular molecule, was common to the reference P1 bacteriophage under GenBank accession no. (jove.com)

Due to the limited resolution of genetic maps that have been established through the use of these approaches, we have constructed a 2-Mb bacterial and P1-derived artificial chromosome (BAC-PAC) contig for the EGFR region and have applied markers positioned on its associated physical map to the analysis of 7p11.2 amplifications in a series of glioblastomas. (elsevier.com)

This inhibition also takes place in the presence of general inhibitors of transcription and/or translation such as the antibiotics rifampin, chloramphenicol, and spectinomycin ( 40 ) and through the inhibition of translation by the Doc protein of prophage P1 ( 21 ). (asm.org)

We have established a protocol for producing libraries of specific mouse chromosomes. (gypsy-blood.com)

Finally, we performed siRNA-mediated knock-down of 31 proteins previously implicated to play a role in X-chromosome inactivation. (ubc.ca)
However, expression levels and patterns of these ectopically expressed N-terminally tagged proteins could differ from those of their endogenous counterparts and thus might cause mislocalization of proteins or artificial interaction with other molecules. (biomedcentral.com)
the c1 repressor of bacteriophage p1 operator-repressor interaction of wild-type and mutant repressor proteins. (liverpool.ac.uk)

In transduction, donor DNA packaged in a bacteriophage infects the recipient bacterium. (nih.gov)
tn10 insertion using phage p1-mediated transduction. (liverpool.ac.uk)

hfr strains of shigella dysenteriae serotype 1 were constructed by transient integration of an rp4 plasmid derivative carrying transposon tn501 into the shigella chromosome through tn501-mediated cointegration. (liverpool.ac.uk)

P1-like phages are part of the mobile elements that carry antibiotic resistance. (jove.com)

Reddy SM, Sun A, Khan OA, Lee LF, Lupiani B. Cloning of a very virulent plus, 686 strain of Marek's disease virus as a bacterial artificial chromosome. (uchicago.edu)

This new form of chromosome engineering, termed recombinogenic engineering or recombineering, is efficient and greatly decreases the time it takes to create transgenic mouse models by traditional means. (nih.gov)
Next, we generated P1 artificial chromosome (PAC) transgenic mice ubiquitously expressing human TFAM. (scienceopen.com)

D' Herelle coined the term bacteriophage meaning 'bacterial eater' to describe the agent's bacteriocidal activity. (slideshare.net)

Bacterial viruses (bacteriophages or phages) have DNA or RNA as genetic material. (nih.gov)

Chromosomes, Artificial, Bacterial" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (uchicago.edu)
Chromosomes, Artificial, Yeast" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings) . (sickkids.ca)

Breeding the transgene to homozygosity results in mice that carry this insertion on both chromosomes, increasing the probability that the insertion will result in an observable phenotype. (mmrrc.org)
This bacteriophage can Here be and Prepare extrachromosomal transgene applications from responsible methods. (zimmervermietung-titze.de)

During backcrossing, the Y chromosome may not have been fixed to the C57BL/6J genetic background. (jax.org)

The method takes advantage of the fact that P1/PAC/BAC's can be isolated as circular DNA molecules, stretched out on glass slides and fine-mapped by multicolor hybridization with smaller probe molecules. (biomedcentral.com)

Such producer strains are usually constructed by sequential chromosome modifications including deletions and integration of genetic material. (biomedcentral.com)

Anatomy51

Organisms37

Diseases17

Chemicals and Drugs44

Analytical, Diagnostic and Therapeutic Techniques and Equipment29

Phenomena and Processes139

Disciplines and Occupations1

Information Science4

Health Care3

A chromosomal region 7p11.2 transcript map: its development and application to the study of EGFR amplicons in glioblastoma. (1/25)

The common retroviral insertion locus Dsi1 maps 30 kilobases upstream of the P1 promoter of the murine Runx3/Cbfa3/Aml2 gene. (2/25)

Genes in a refined Smith-Magenis syndrome critical deletion interval on chromosome 17p11.2 and the syntenic region of the mouse. (3/25)

Transcriptional regulation of the stem cell leukemia gene (SCL)--comparative analysis of five vertebrate SCL loci. (4/25)

Prospective screening for subtelomeric rearrangements in children with mental retardation of unknown aetiology: the Amsterdam experience. (5/25)

Comparative genomic sequence analysis of the human chromosome 21 Down syndrome critical region. (6/25)

Annotation and BAC/PAC localization of nonredundant ESTs from drought-stressed seedlings of an indica rice. (7/25)

Evidence for a fast, intrachromosomal conversion mechanism from mapping of nucleotide variants within a homogeneous alpha-satellite DNA array. (8/25)

Bacteriophages

Chromosomes

Chromosome Mapping

Bacteriophage T4

Bacteriophage lambda

Coliphages

Bacteriophage T7

Chromosome Banding

Lysogeny

X Chromosome

T-Phages

Chromosome Aberrations

Bacteriophage mu

Chromosomes, Bacterial

Sex Chromosomes

Chromosomes, Human, Pair 1

Escherichia coli

DNA, Viral

Chromosomes, Human

Bacteriophage phi 6

Chromosome Segregation

Chromosomes, Human, Pair 7

Bacteriophage phi X 174

Chromosomes, Human, Pair 11

Chromosome Deletion

Chromosomes, Human, Pair 17

Molecular Sequence Data

Viral Proteins

Base Sequence

Chromosomes, Human, Pair 6

Bacteriophage P2

Chromosomes, Human, Pair 9

Chromosomes, Human, Pair 21

Mutation

Chromosomes, Plant

Bacteriophage M13

Chromosomes, Fungal

DNA Viruses

Chromosomes, Human, 6-12 and X

Bacteriophage T3

Bacteriophage P1

Chromosomes, Human, Pair 2

Chromosomes, Human, Pair 16

Chromosomes, Human, Pair 22

Chromosome Pairing

Bacteriophage Typing

Chromosomes, Mammalian

Chromosomes, Human, Pair 13

Chromosomes, Human, Pair 4

Chromosomes, Human, Pair 10

Chromosomes, Human, Y

Chromosomes, Human, Pair 8

Chromosomes, Human, Pair 19

Chromosome Disorders

Salmonella Phages

Chromosomes, Artificial, Bacterial

Recombination, Genetic

Siphoviridae

Chromosomes, Human, X

Chromosomes, Human, 1-3

Chromosomes, Human, Pair 12

Chromosome Painting

Chromosomes, Human, Pair 5

Genes, Viral

Chromosomes, Human, Pair 15

RNA Phages

Karyotyping

Chromosomes, Human, Pair 14

Chromosomes, Human, Pair 18

Bacteriolysis

Staphylococcus Phages

Chromosomes, Human, 16-18

Genetic Linkage

Bacteriophage PRD1

Pseudomonas Phages

In Situ Hybridization, Fluorescence

Chromosomes, Human, Pair 20

Chromosomes, Artificial, Yeast

DNA Replication

Bacillus Phages