A technique with which an unknown region of a chromosome can be explored. It is generally used to isolate a locus of interest for which no probe is available but that is known to be linked to a gene which has been identified and cloned. A fragment containing a known gene is selected and used as a probe to identify other overlapping fragments which contain the same gene. The nucleotide sequences of these fragments can then be characterized. This process continues for the length of the chromosome.
An activity in which the body advances at a slow to moderate pace by moving the feet in a coordinated fashion. This includes recreational walking, walking for fitness, and competitive race-walking.
Any method used for determining the location of and relative distances between genes on a chromosome.
A genus of fungi in the family Ganodermataceae, order POLYPORALES, containing a dimitic hyphal system. It causes a white rot, and is a wood decomposer. Ganoderma lucidum (REISHI) is used in traditional Chinese medicine (MEDICINE, CHINESE TRADITIONAL).
In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)
Plasmids containing at least one cos (cohesive-end site) of PHAGE LAMBDA. They are used as cloning vehicles.
Mapping of the linear order of genes on a chromosome with units indicating their distances by using methods other than genetic recombination. These methods include nucleotide sequencing, overlapping deletions in polytene chromosomes, and electron micrography of heteroduplex DNA. (From King & Stansfield, A Dictionary of Genetics, 5th ed)
The insertion of recombinant DNA molecules from prokaryotic and/or eukaryotic sources into a replicating vehicle, such as a plasmid or virus vector, and the introduction of the resultant hybrid molecules into recipient cells without altering the viability of those cells.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
A phenotypically recognizable genetic trait which can be used to identify a genetic locus, a linkage group, or a recombination event.
The sequence of PURINES and PYRIMIDINES in nucleic acids and polynucleotides. It is also called nucleotide sequence.
Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.
The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.
Use of restriction endonucleases to analyze and generate a physical map of genomes, genes, or other segments of DNA.
Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.
The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed)
A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.
Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
Very long DNA molecules and associated proteins, HISTONES, and non-histone chromosomal proteins (CHROMOSOMAL PROTEINS, NON-HISTONE). Normally 46 chromosomes, including two sex chromosomes are found in the nucleus of human cells. They carry the hereditary information of the individual.
Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.
The orderly segregation of CHROMOSOMES during MEIOSIS or MITOSIS.
A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.
A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.
A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
A specific pair GROUP C CHROMSOMES of the human chromosome classification.
Actual loss of portion of a chromosome.
A specific pair of GROUP C CHROMSOMES of the human chromosome classification.
A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.
Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of PLANTS.
Structures within the nucleus of fungal cells consisting of or containing DNA, which carry genetic information essential to the cell.
The medium-sized, submetacentric human chromosomes, called group C in the human chromosome classification. This group consists of chromosome pairs 6, 7, 8, 9, 10, 11, and 12 and the X chromosome.
A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.
A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.
A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.
The alignment of CHROMOSOMES at homologous sequences.
Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of MAMMALS.
A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.
A specific pair of GROUP B CHROMOSOMES of the human chromosome classification.
A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.
The human male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans.
A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.
A specific pair of GROUP F CHROMOSOMES of the human chromosome classification.
Lists of words, usually in alphabetical order, giving information about form, pronunciation, etymology, grammar, and meaning.
A genus herbs of the Asteraceae family. The SEEDS yield oil and are used as food and animal feed; the roots of Helianthus tuberosus (Jerusalem artichoke) are edible.
The failure of PLANTS to complete fertilization and obtain seed (SEEDS) as a result of defective POLLEN or ovules, or other aberrations. (Dict. of Plant Genet. and Mol. Biol., 1998)
The capacity to conceive or to induce conception. It may refer to either the male or female.
A plant genus of the family BRASSICACEAE known for its peppery red root.
A plant genus of the family FABACEAE that is used for food in NIGERIA.
A plant genus of the family Plantaginaceae. The small plants usually have a dense tuft of basal leaves and long, leafless stalks bearing a terminal spike of small flowers. The seeds, known as PSYLLIUM, swell in water and are used as laxatives. The leaves have been used medicinally.

A sequence-ready BAC clone contig of a 2.2-Mb segment of human chromosome 1q24. (1/186)

Human chromosomal region 1q24 encodes two cloned disease genes and lies within large genetic inclusion intervals for several disease genes that have yet to be identified. We have constructed a single bacterial artificial chromosome (BAC) clone contig that spans over 2 Mb of 1q24 and consists of 78 clones connected by 100 STSs. The average density of mapped STSs is one of the highest described for a multimegabase region of the human genome. The contig was efficiently constructed by generating STSs from clone ends, followed by library walking. Distance information was added by determining the insert sizes of all clones, and expressed sequence tags (ESTs) and genes were incorporated to create a partial transcript map of the region, providing candidate genes for local disease loci. The gene order and content of the region provide insight into ancient duplication events that have occurred on proximal 1q. The stage is now set for further elucidation of this interesting region through large-scale sequencing.  (+info)

Genomic organization and localization of the human CRMP-1 gene. (2/186)

The Collapsin Response Mediator Protein-1 (CRMP-1) is a brain specific protein considered to be involved in the collapsin-induced growth cone collapse during neural development. CRMP-1 belongs to the Unc-33 gene family. Here we report the genomic structure and the localization of the human CRMP-1 gene to chromosome 4p16.1. Sequence analysis revealed that the human CRMP-1 gene consists of 14 exons. We have also established sequencing assays for all its coding exons. This should permit the rapid screening for mutations to assess CRMP-1 role in genetic disorders mapped in the 4p16.1 region.  (+info)

Analyses of a polyhydroxyalkanoic acid granule-associated 16-kilodalton protein and its putative regulator in the pha locus of Paracoccus denitrificans. (3/186)

The polyhydroxyalkanoic acid (PHA) granule-associated 16-kDa protein (GA16 protein) of Paracoccus denitrificans was identified, and its corresponding gene was cloned and analyzed at the molecular level. The N-terminal amino acid sequence of GA16 protein revealed that its structural gene is located downstream from the PHA synthase gene (phaCPd) cloned recently (S. Ueda, T. Yabutani, A. Maehara, and T. Yamane, J. Bacteriol. 178:774-779, 1996). Gene walking around phaCPd revealed two new open reading frames (ORFs) possibly related to PHA synthesis, one of which was the phaPPd gene, encoding GA16 protein, and the other was the phaRPd gene, encoding a protein that is putatively involved in the regulation of the expression of phaPPd. Overproduction of PhaPPd was observed in Escherichia coli carrying phaPPd, but the overproduction was not observed in the presence of phaRPd. Coexpression of phaPPd and PHA biosynthesis genes in E. coli caused increases in both the number of poly-(3-hydroxybutyric acid) (PHB) granules and PHB content and caused decreases in both the size of the granules and the molecular weight of PHB. GA16 protein was considered a phasin protein. The phaRPd gene had significant similarities to stdC, a possible transcriptional factor of Comamonas testosteroni, as well as to other ORFs of unknown function previously found in other PHA-synthetic bacteria.  (+info)

Breaking colinearity in the mouse HoxD complex. (4/186)

Vertebrate Hox genes are activated in a spatiotemporal sequence that reflects their clustered organization. While this colinear relationship is a property of most metazoans with an anterior to posterior polarity, the underlying molecular mechanisms are unknown. Previous work suggested that Hox genes were made progressively available for transcription in the course of gastrulation, implying the existence of an element capable of initiating a repressive conformation, subsequently relieved from the clusters sequentially. We searched for this element by combining a genomic walk with successive transgene insertions upstream of the HoxD complex followed by a series of deletions. The largest deficiency induced posterior homeotic transformations coincidentally with an earlier activation of Hoxd genes. These data suggest that a regulatory element located upstream of the complex is necessary for setting up the early pattern of Hox gene colinear activation.  (+info)

The type II pullulanase of Thermococcus hydrothermalis: molecular characterization of the gene and expression of the catalytic domain. (5/186)

The gene encoding a hyperthermostable type II pullulanase produced by Thermococcus hydrothermalis (Th-Apu) has been isolated. Analysis of a total of 5.2 kb of genomic DNA has revealed the presence of three open reading frames, one of which (apuA) encodes the pullulanase. This enzyme is composed of 1,339 amino acid residues and exhibits a multidomain structure. In addition to a typical N-terminal signal peptide, Th-Apu possesses a catalytic domain, a domain bearing S-layer homology-like motifs, a Thr-rich region, and a potential C-terminal transmembrane domain. The presence of these noncatalytic domains suggests that Th-Apu may be anchored to the cell surface and be O glycosylated.  (+info)

Physical map and organization of chromosome 7 in the rice blast fungus, Magnaporthe grisea. (6/186)

The rice blast fungus Magnaporthe grisea is a highly destructive plant pathogen and one of the most important for studying various aspects of host-plant interactions. It has been widely adopted as a model organism because it is ideally suited for genetic and biological studies. To facilitate map-based cloning, chromosome walking, and genome organization studies of M. grisea, a complete physical map of chromosome 7 was constructed using a large-insert (130 kb) bacterial artificial chromosome (BAC) library. Using 147 chromosome 7-specific single-copy BAC clones and 20 RFLP markers on chromosome 7, 625 BAC clones were identified by hybridization. BAC clones were digested with HindIII, and fragments were size separated on analytical agarose gels to create DNA fingerprints. Hybridization contigs were constructed using a random cost algorithm, whereas fingerprinting contigs were constructed using the software package FPC. Results from both methods were generally in agreement, but numerous anomalies were observed. The combined data produced five robust anchored contigs after gap closure by chromosomal walking. The genetic and physical maps agreed closely. The final physical map was estimated to cover >95% of the 4.2 Mb of chromosome 7. Based on the contig maps, a minimum BAC tile containing 42 BAC clones was created, and organization of repetitive elements and expressed genes of the chromosome was investigated.  (+info)

A 12-Mb complete coverage BAC contig map in human chromosome 16p13.1-p11.2. (7/186)

We have constructed a complete coverage BAC contig map that spans a 12-Mb genomic segment in the human chromosome 16p13.1-p11.2 region. The map consists of 68 previously mapped STSs and 289 BAC clones, 51 of which-corresponding to a total of 7.721 Mb of genomic DNA-have been sequenced, and provides a high resolution physical map of the region. Contigs were initially built based mainly on the analysis of STS contents and restriction fingerprint patterns of the clones. To close the gaps, probes derived from BAC clone ends were used to screen deeper BAC libraries. Clone end sequence data obtained from chromosome 16-specific BACs, as well as from public databases, were used for the identification of BACs that overlap with fully sequenced BACs by means of sequence match. This approach allowed precise alignment of clone overlaps in addition to restriction fingerprint comparison. A freehand contig drawing software tool was developed and used to manage the map data graphically and generate a real scale physical map. The map we present here is approximately 3.5 x deep and provides a minimal tiling path that covers the region in an array of contigous, overlapping BACs.  (+info)

Characterization of the DNA polymerase loci of the novel porcine lymphotropic herpesviruses 1 and 2 in domestic and feral pigs. (8/186)

Two novel porcine gammaherpesviruses, porcine lymphotropic herpesviruses 1 and 2 (PLHV-1 and -2), have been detected by amplification of short DNA polymerase (DPOL) sequences from blood and spleen of domestic pigs while searching for unknown herpesviruses in pigs as possible risk factors in xenotransplantation. In the present study, the DPOL genes of the two viruses and the open reading frames (ORFs) that follow in the downstream direction were amplified by PCR-based genome walking from adaptor-ligated restriction fragment libraries of porcine spleen samples. The sequences determined for the two PLHVs exhibited a very low G+C content (37 mol%) and a marked suppression of the CpG dinucleotide frequency. The DPOL proteins encoded were 95% identical and showed a close relationship (60% identity) to the DPOL protein of a ruminant gammaherpesvirus, alcelaphine herpesvirus 1 (AlHV-1). This was confirmed by phylogenetic analyses of the conserved regions of the two PLHV DPOL proteins. The PLHV ORFs downstream of DPOL exhibited 83% identity to each other and >>50% similarity to ORF A5, the position equivalent of AlHV-1. From these data, the PLHVs can be firmly classified to the subfamily Gammaherpesvirinae: To find a natural reservoir for the PLHVs, organs of feral pigs were screened with five different PCR assays, targetting either the DPOL gene or 3'-flanking sequences. In all samples, PLHV sequences were detected that originated predominantly from PLHV-2, suggesting the possibility of virus transfer between feral and domestic pig populations.  (+info)

Everybodys looking for the perfect fitness tips. We have five super walking strategies that will help you make the most of your walking workout.
Zebrafish genome sequencing project has improved efficiency of positional cloning in zebrafish and traditional chromosome walking by isolating large insert genomic libraries has become a past. However, the genetic principles underlying the positional cloning still form the foundation for current chromosome walking using the genome sequence assemblies and related genomic sequence and clone information. This guide intends to summarize our accumulated experience in positional cloning using the current genomic resources and tools, and provide a practical guide to positional and/or candidate cloning of mutants of interest ...
The Arabidopsis mutants designated gly1 exhibit a reduced carbon flux through the prokaryotic pathway that is compensated for by an increased carbon flux through the eukaryotic pathway. Biochemical approaches reveal that the gly1 phenotype cannot be explained by a deficiency in the enzymes of the prokaryotic pathway. The chemical complementation of the mutant phenotype by exogenous glycerol treatment of gly1 plants suggests a lesion affecting the glycerol 3-phosphate supply within the chloroplast. As an alternative to the biochemical study of the gly1 mutants we set out to map the GLY1 locus. The gly1 mutant being an EMS (ethyl methane sulphonate) mutant, we used a strategy based on the polymorphism existing between Arabidopsis ecotypes, here Columbia (gly1 background) and Landsberg erecta. We mapped gly1 on chromosome II. During the process of chromosome walking, the complete sequence of chromosome II was released, allowing us to make assumptions on candidate genes based on map location. We are ...
Postdoctoral Position Hong Kong Univ. Sci. & Technol. To investigate the molecular mechanisms of ABA signal transduction. We are chromosome walking to Arabidopsis mutants that ectopically express an ABA-inducible reporter and have pleiotropic effects on morphogenesis (Planta 210: 875 and abstract #232, http://www.wisc.edu/union/info/conf/arabidopsis/reginfo_arab.html). A second project involves flow cytometry, electrophysiology, and biochemistry of rice protoplasts that express ABA-inducible GFP. We are focusing on the role of a cell surface glycoprotein that may interact with an ABA receptor (FEBS Lett. 456: 257), and the mechanism of action of trivalent ions (Plant Physiol., in press). Salary is US$37,100 per annum plus benefits. Send email with research interests and experience, career goals, curriculum vitae, and names of 3 references to: Chris Rock, Dept. Biology, HKUST, Clear Water Bay, Kowloon, Hong Kong. Email: borock at ust.hk; http://www.ust.hk/~webbo/. ...
Steve Jackson Games is raising funds for Ogre Miniatures Set 2 on Kickstarter! Plastic Ogre miniatures for use with the Ogre Sixth Edition and Ogre Designers Edition games.
Fueled by the prospect of an empty cupboard, the ogre started toward her. She looked at the staff and the thought of wind blew into her head. Lelani lifted the staff above her and swung it in a circle like she was stirring the sky. Gusts began to spin around the ogre, kicking up sand and dirt into its one good eye. The ogre covered its face with one hand and reached around blindly to grab Lelani, but she had already taken to the path, sure that her friends were far away by now. As she galloped, the canyon walls grew closer together. Ahead, it came to a dead end except for a small opening in the wall. Klaugh waited for her on the other side. She ran for it with all her might, certain that the monster was right behind her. ...
PubMed comprises more than 30 million citations for biomedical literature from MEDLINE, life science journals, and online books. Citations may include links to full-text content from PubMed Central and publisher web sites.
The RCP has released its consultation response to the UK governments call for suggestions and evidence of innovative projects and programmes for its cycling and walking strategy. ...
The RCP has released its consultation response to the UK governments call for suggestions and evidence of innovative projects and programmes for its cycling and walking strategy. ...
Heavy Movement - Ogre: Large tanks are big enough to ignore some terrain effects. Like tanks, they too rely on caterpillar treads to cross difficult terrain, but due to the...
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Ive finally had the chance to show this off in my office after buying 6th edition (my coworkers scoffed at the massive designers box- they equated size with complexity). It got me thinking about the game again. The engine is pretty sound, and apart from odds computation (something not
A Legion themed expansion may or may not be coming. That said, regarding new races, there are some races iconic to both sides that each wants for different reasons but that still havent made their way to become playable. For Alliance the culprit is high elves, and for the Horde its ogres. Players from both sides have expressed their desire to see these races added time and time again, even if some dont want to. The problem: - for high elves the most clear problem is that they would
All commentary contained herein is copyrighted. Please contact us for usage rights and conditions. We arent mean ogres, we just want to make sure that our material isnt stolen as there are those out there in this cruel world who would do just that. ...
All commentary contained herein is copyrighted. Please contact us for usage rights and conditions. We arent mean ogres, we just want to make sure that our material isnt stolen as there are those out there in this cruel world who would do just that. ...
ASKEP FRAKTURA. DEFINISI Fraktur adalah putusnya kontinuitas tulang dan ditentukan sesuai tipe dan luasnya. Fraktur terjadi ketika tulang diberik...
Looking for online definition of chromosome walking in the Medical Dictionary? chromosome walking explanation free. What is chromosome walking? Meaning of chromosome walking medical term. What does chromosome walking mean?
Yeast artificial chromosomes (YACs) are genetically engineered chromosomes derived from the DNA of the yeast, Saccharomyces cerevisiae, which is then ligated into a bacterial plasmid. By inserting large fragments of DNA, from 100-1000 kb, the inserted sequences can be cloned and physically mapped using a process called chromosome walking. This is the process that was initially used for the Human Genome Project, however due to stability issues, YACs were abandoned for the use of Bacterial artificial chromosomes (BAC). Beginning with the initial research of the Rankin et al., Strul et al., and Hsaio et al., the inherently fragile chromosome was stabilized by discovering the necessary autonomously replicating sequence (ARS); a refined YAC utilizing this data was described in 1983 by Murray et al. The primary components of a YAC are the ARS, centromere, and telomeres from S. cerevisiae. Additionally, selectable marker genes, such as antibiotic resistance and a visible marker, are utilized to select ...
Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence into several consecutive short ones. The DNA of interest may be a plasmid insert, a PCR product or a fragment representing a gap when sequencing a genome. The term primer walking is used where the main aim is to sequence the genome. The term chromosome walking is used instead when the sequence is known but there is no clone of a gene. For example, the gene for a disease may be located near a specific marker such as an RFLP on the sequence. The fragment is first sequenced as if it were a shorter fragment. Sequencing is performed from each end using either universal primers or specifically designed ones. This should identify the first 1000 or so bases. In order to completely sequence the region of interest, design and synthesis of new ...
1. Introduction to Genetic Engineering, 2. Concepts of Genetic Engineering, 3. Methodology of Genetic Engineering, 4. Construction of Recombinant DNA, 5. Gene Cloning, 6. Tools used in Genetic Engineering, 7. Linking of Desired Gene with Vector DNA, 8. Gene Cloning Vectors, 9. In vitro Construction of pBR322, 10. In vitro construction of Cosmid, 11. Preparation of Desired DNA, 12. Introduction of rDNAs into Host Cells, 13. Selection of Recombinants, 14. Expression of Cloned Genes, 15. Polymerase Chain Reaction (PCR), 16. Blotting Techniques, 17. DNA Sequencing, 18. Molecular Markers and their Applications, 19. Genomic Library, 20. cDNA Libraries, 21. Chromosome Walking, 22. Chromosome Jumping, 23. Genetically Engineered Microorganisms, 24. Transgenic Plants, 25. Transgenic Animals, 26. Biohazards of Recombinant DNA Technology, 27. Applications of Genetic Engineering, 28. Glossary ...
Principles and targets of the strategy will include: 80% of children walking to school; creating the10 minute city - where trips can be made through a continuous network; and undertaking universal design principles to achieve their goals. A 2009 travel survey showed that 16% of Norwegians did not report any journeys on foot, while 68% reported walking for part of their journey. The average Norwegian walks approximately 1.7 km/day; 51% walk less than 500 m per day (including walking to other means of transport); while 19% walk 1.5 km or more. Wouldnt it be something if Canada were to undertake a national walking strategy or even if our individual provinces undertook this work!. Throughout the conference there was general agreement that far more attention needs to be paid to vulnerable road users - children and seniors - and that walking should be a human right and given the highest priority in transportation planning. One speaker noted that by 2050 22% of the worlds population will be over ...
The car-sharing concept originated in Switzerland in 1987, and one of the largest car-sharing opera- tions in the world is still run by Mobility Switzerland, in close partnership with the Swiss Federal Railway. It runs a fleet of 1,650 cars in more than 930 locations, and has enrolled more than 58,000 members. As well as its size, the Swiss program has enjoyed enormous success in influencing travel behavior. Surveys show that members who gave up their car after joining the car-sharing program increased their transit usage by 35%, from 3,560 miles per year to 4,810 miles per year. ...
Bailey, I.E., Myatt, J.P. and Wilson, A.M. (2013). Group hunting within the carnivora: physiological, cogntivie and environmental influences on strategy and cooperation. Behav. Ecol. Sociobiol. 67: 1-17.. Usherwood, J.R., Channon, A.J., Myatt, J.P., Rankin, J.W. and Hubel, T.Y. (2012). The human foot and heel-sole-toe walking strategy: a mechanism enabling an inverted pendular gait with low isometric force?. J. Roy. Soc. Interface.. van Casteren, A; Sellers, W.I., Thorpe, S.K.S., Coward, S., Crompton, R.H., Myatt, J.P. and Ennos, A.R. (2012). Nest-building orangutans demonstrate engineering know-how to produce safe, comfortable beds. Proc. Natl. Acad. Sci.. Myatt, J.P., Crompton, R.H. and Payne-Davis et al. (2012). Functional adaptation in the forelimb muscles of non-human great apes. J Anat.. King A.J., Cheng, L., Starke, S.D. and Myatt, J.P. (2011). Is the true wisdom of the crowd to copy successful individuals?. Biol Lett.. Myatt J.P. and Thorpe, S.K.S. (2011). Postural strategies employed ...
Explicit MSAA finally arrived in Ogre 2.2; and thanks to load and store actions; you have a lot of control over what happens with MSAA and when; which can result in high quality rendering by making MSAA a first class citizen.. Were also planning on telling you where the subsamples are located; which can help with certain algorithms that require this information (such as SMAA S2x). We also want to support programmable subsample locations, but unfortunately DX11 does not expose it unless we use vendor-specific extensions (which were actually evaluating to support them…) and not all GPUs support them either.. Programmable sample locations is a huge deal. For example it makes low res particle FXs work much better: By using a half resolution MSAA 4x surface with subsamples set to center; it is possible to shade colour at half resolution without pixelization or halo artifacts at the borders because depth discontinuities are evaluated at full resolution. Console games are already starting to do ...
Download Ogre3d Python bindings (Python-Ogre) for free. Python bindings for Ogre3d graphics engine, built using Py++ and Boost.Python library - Also refered to a Python-Ogre.
ID PTT1 preliminary; circular DNA; SYN; 5025 BP. XX AC IG9906; XX DT 01-JUL-1995 (Rel. 11, Created) DT 01-JUL-1995 (Rel. 12, Last updated, Version 1) XX DE E. coli plasmid vector pTT1 - complete. XX KW cloning vector. XX OS Cloning vector OC Artificial sequences; Cloning vehicles. XX RN [1] RC pTT1 from pKK233-2 RC pTT3 from pTT1 & pUCP18 RC pTT3deltaS from pTT3 RC pTT4 from pTT3deltaS RC pPZ1 from pNM480 & pUCP19 RC pPZ2 from pNM481 & pUCP19 RC pPZ3 from pNM482 & pUCP19 RC pPZ10 from pNM480 & pTT4 RC pPZ20 from pNM481 & pTT4 RC pPZ30 from pNM482 & pTT4 RC pRZ1 from pDF202 & pPZ3 RA Schweizer H.P.; RT Improved broad-host-range lac-based plasmid vectors for the RT isolation and characterization of protein fusions in Pseudomonas RT aeruginosa; RL Gene 103:87-92(1991). XX CC Created by Moore, July 1995, under contract with NCBI. CC NM (pTT1) CC CM (yes) CC NA (ds-DNA) CC TP (circular) CC ST () CC TY (plasmid) CC SP () CC HO (E.coli) CC CP () CC FN (cloning) CC SE () CC PA (pKK233-2)(pUCP18) CC BR ...
ADDITIONAL INFORMATION Product Type Brand Korea Model Number 103496244 Manufacturer 포나미 Info. DETAILED IMAGES PRODUCT INFORMATION Product Name and Model No. Ref…
Cast: Mike Myers, Eddie Murphy, Cameron Diaz, Antonio Banderas, Walt Dohrn, Jon Hamm, Jane Lynch, Craig Robinson, Julie Andrews, John Cleese. The final chapter of the Shrek series asks an interesting question: what kind of world would Shrek live in if he never met Fiona and his friends?. Shrek Forever After follows the green ogre during a mid-life crisis; he is now a bored and domesticated family man who also happens to be a tourist attraction. Wishing he could feel like a real ogre again for just a day, Rumplestiltskin (Dohrn) overhears this and grants Shrek his wish. He sends the ogre to an alternate world - a world without him in it. However, once Shrek realizes he never existed in a Rumplestiltskin-ruled Far Far Away, its up to him to restore things back to how they used to be before its too late.. Shrek Forever After is better than Shrek The Third, but not by much. The idea of using time-travel is a clever way to try and refresh the Shrek franchise. In the new world, Puss is morbidly ...
The role of historical contingency in evolution has been much debated, but rarely tested. Twelve initially identical populations of Escherichia coli were founded in 1988 to investigate this issue. They have since evolved in a glucose-limited medium that also contains citrate, which E. coli cannot use as a carbon source under oxic conditions. No population evolved the capacity to exploit citrate for >30,000 generations, although each population tested billions of mutations. A citrate-using (Cit+) variant finally evolved in one population by 31,500 generations, causing an increase in population size and diversity. The long-delayed and unique evolution of this function might indicate the involvement of some extremely rare mutation. Alternately, it may involve an ordinary mutation, but one whose physical occurrence or phenotypic expression is contingent on prior mutations in that population. We tested these hypotheses in experiments that ‘‘replayed’’ evolution from ...
redicament? Because smoking the cigarette is the end to a means. That ends to a means is always to meet a physical craving, peaceful your nerves, stop you from consuming, whatever purpose you use to warrant the smoking. Its actually the device, the suppression of the bodys defensives, that the smoker has spent therefore long producing that enables for them and now causes the smoker to see the need to smoke. In the event that you are able to quit cigarettes but do not eliminate this body reduction trigger, the most effective you are able to hope for is to be an ex-smoker. What precisely is definitely an ex-smoker? An ex-smoker is an individual who has smoked previously and has brought a break from smoking, but will likely resume smoking in the future.. The defense mechanism that you spent decades gathering remains functioning. It is becoming your ogre that you have forgotten how to be responsible for! You have cleared from your own memory why you developed REV GTS TC Box Mod ogre initially, ...
Do you like this ogre? . He looks awesome. Don´t you think? . Now you can create your own Shrek. Choose a background and an audio message , upload a photo and you have a new version of the famous ogre. Get ready for Shrek Forever After with this fun application for Paramount. ...
PaV: I suppose for me to be pried away from what I do to focus long and hard on that particular problem would take, quite honestly, hundreds of thousands of dollars to begin to pique my interest. ...
On the one hand, I understand that the authors of this volume probably believe that they cannot get their work published in conventional biology journals, because of their controversial, anti-evolution conclusions. I completely sympathize. I would love to be able to have some of my creationist ideas intelligently read and critiqued by knowledgeable individuals, rather than dismissively scoffed at by howler monkeys (you gotta be a real oldtimer to remember that reference). On the other hand, Im a firm believer in the value of peer review and scientific publication. If a work is rejected, theres probably a reason for the rejection that we should take seriously. Scientific publication isnt just some political game, where friends get published and enemies get punished. Its not an inalienable right either. If we dont respect the process of peer review and publication, then whats the difference between a scientific publication and a propaganda piece? The price tag ...
Movie info: A bored and domesticated Shrek pacts with deal-maker Rumpelstiltskin to get back to feeling like a real ogre again, but when hes duped and sent to a twisted version of Far Far Away-where Rumpelstiltskin is king, ogres are hunted, and he and Fiona have never met-he sets out to restore his world and reclaim his true love.
an asterisk starts an unordered list * and this is another item in the list + or you can also use the + character - or the - character To start an ordered list, write this: 1. this starts a list *with* numbers + this will show as number 2 * this will show as number 3. 9. any number, +, -, or * will keep the list going. * just indent by 4 spaces (or tab) to make a sub-list 1. keep indenting for more sub lists * here im back to the second level ...
It is looted from Knot Thimblejacks Cache. In the Other Trade Goods category. An item from Classic World of Warcraft. Always up to date with the latest patch.
What if I told you that Ive crossed the border of forever, And made it back, unscathed and undetected, With a bulging sack full of looted time? What if I told you that I dont even need to hide; For the ogre sublime will never even know that its missing, And wouldnt even know who…
By Kevin Crust HollywoodNews.com: A soft May could turn into June gloom at the box-office if the pre-Toy Story (June 18) releases all fail to break out. Four new movies debut this week, but will all likely fall behind the ogre who looking to three-peat at number one. GET HIM TO THE ...
Looking for fitness professionals who are skilled in walking? . Youve come to the right place. Find quality professionals who specialize in walking here.
Guided walking in Grazalema. Explore this isolated region and enjoy challenging walking read more to discover the practicalities, the best time to go and advice from our travellers on guided walking in Grazalema.
Walking improves your sex life and makes you live longer.And if you don`t go for a deal like that, turn to the obit page and look for your name.The beneficial aspects of walking should be familiar
Whether youre race-walking or just walking to get healthy, learn more about the low-impact form of fitness and find walking events perfect for you.
Credible: Worthy of belief or confidence; trustworthyCredibility: Something that takes considerable time and effort to achieve, but can be lost in an
What are the differences between compostable, biodegradable or flushable dog poop bags - and whats the truly greenest to use when out walking your pooch?
In Sunflower hybrids are obtained by using the cytoplasmic male sterility/fertility restoration system, which is due to a nuclear-cytoplasmic incompatibility. Hybrid breeding is based on the cytoplasmic male sterility, PET1. Male fertility can be restored by the nuclear restorer gene Rf1. The objective of this study was to use a map-based cloning strategy to isolate the Rf1 gene. Seventy-one BAC-clones were identified. Twenty-seven BAC-ends were isolated. Contigs were formed, each representing a unique genomic region. The two BAC libraries used have a too low coverage in the region of Rf1 gene.
POSTDOCTORAL POSITIONS AVAILABLE Riverside, California, USA Molecular genetics of the oomycete fungus, Phytophthora infestans Candidates are sought for two postdoctoral positions to study the molecular genetics of Phytophthora infestans, an oomycete fungus that causes the late blight diseases of potato and tomato. Positions are available in either of several projects. These include the positional cloning of fungicide resistance genes, the characterization of genes involved in sexual development and sporulation, and comparative studies of the structure and expression of mating type loci throughout the genus. Funding for these positions come from sources that include USDA and NSF grants. Additional information about the laboratory is available at: http://cnas.ucr.edu/~ppath/faculty/hj.html. Applicants should have experience in molecular biological techniques. Depending on the project, the experiments will involve methods such as genetic mapping, chromosome walking using BAC libraries, fungal ...
The dwarf phenotype is one of the important traits for plant architecture, and it can increase plant density and land utilization especially for protected cultivation. Several dwarf genes have been identified in watermelon, but most of them were not mapped or cloned. In this study, the Cldw1 gene was primary mapped on watermelon chromosome 9 by bulked segregate analysis (BSA-seq) using F2 plants derived from the cross of a normal line WT4 and a dwarf line WM102. The candidate region identified by BSA-seq was subsequently validated and confirmed by linkage analysis using 30 SSR markers in a F2 population with 124 plants. The Cldw1 gene was further fine mapped in a large population with 1053 F2 plants by chromosome walking, and it was delimited into a candidate region of 106 kb. There were six gene predicted in the candidate region and only the gene Cla010337 was identified with two SNPs and a single nucleotide deletion in the coding region in WM102. A dCAPS marker was developed from the single nucleotide
Bleomycin is a broad-spectrum glycopeptide antitumor antibiotic produced by Streptomyces verticillus. Clinically, the mixture of bleomycin A2 and bleomycin B2 is widely used in combination with other drugs for the treatment of various cancers. As a secondary metabolite, the biosynthesis of bleomycin is precisely controlled by the complex extra-/intracellular regulation mechanisms, it is imperative to investigate the global metabolic and regulatory system involved in bleomycin biosynthesis for increasing bleomycin production. N-acetylglucosamine (GlcNAc), the vital signaling molecule controlling the onset of development and antibiotic synthesis in Streptomyces, was found to increase the yields of bleomycins significantly in chemically defined medium. To mine the gene information relevant to GlcNAc metabolism, the DNA sequences of dasR-dasA-dasBCD-nagB and nagKA in S. verticillus were determined by chromosome walking. From the results of Real time fluorescence quantitative PCR (RT-qPCR) and
We had a [15-year] goal to ultimately work out all the genes by about 2004, said Gilbert, who chairs the biology department.. According to Patrick M. Gillevet, director and chief scientist of the Harvard Genome Laboratory, Gilberts project was to develop a direct sequencing method, using bacteria as models.. Its a genomic walking strategy, he said. We take the whole genome of this bacteria and walk along it, and actually sequence it directly.. Gilbert will not be the only one affected by the loss. A total of 15 scientists work in the genome laboratory, including four computer lab technicians, eight bench technicians and three professional scientists. Because the lab is closing, many of them have been forced to find new employment.. It was panicked there for a while, but things have calmed down a bit, now that people have found jobs, Gillevet said.. Gillevet said he will move elsewhere to continue the project. I think [the technicians] feel a little bit better that Im bringing the ...
Khurasan Miniatures have added a new post to their blog with a new render of their Ogre Beetle figure and additional background for the miniature.. ...
One of the most powerful and underrated ways to lose weight, stay healthy and live longer is an activity we do every day - walking. Even walking for an average of only 30 minutes a day can have huge health benefits, such as lowering your risk of stroke by 35% and Type 2 diabetes by 40%. The best thing about walking is that its free and easy to fit into your daily routine.. Here are some benefits of walking from the home doctor experts at House Call Doctor.. It can help you manage your weight. Walking is a great way to burn calories. This will depend on a few factors, including your walking speed, distance covered, terrain and your weight. Interval walking is a great way to burn the most calories. For example, you can experiment with walking fast for a minute and then cool down for two minutes until you reach 30 minutes total.. It can improve your mood. Walking can greatly improve your mental health. Aim for 30 minutes of brisk walking to help reduce anxiety, depression and a negative mood. It ...
Hi, once again we at MagicBlogs.de can show you an exclusive preview card from the upcoming edition: http://forums.mtgsalvation.com/showthread.php?t=...
Choroideremia is a rare inherited disorder that causes severe sight loss progressively throughout ones life, primarily within men.
Tod Purvis and his brother Justin were diagnosed with choroideremia and took a road trip chronicled in the movie Driving Blind.
I have contact to a group of game-devs, that just switch from Ogre to Unreal. After making some demo-meshes for them, this change occurs. Sure i tried to get familiar with the UDK - with no success. As in NeoAxis, i dont get my models to my eyes with all that fantastic new material features. The Unreal-material-editor is awful, and i even dont like this hyper-real-look of rendered objects. My style is heavy based on the pore texture, although i would like to add some reflections etc ...
We have suffered many losses, ,name,. Dreadmaul Hold has sent ogres from the Blasted Lands as reinforcements, but they can only send so many; the Alliance attacks them as well, even as we speak ...
Find 1000s of Walking Mouse pads. Many colors, styles, patterns, & designs available, or make your own custom personalized mousepad with a photo or design.
Find over 5 Walking in the City groups with 7196 members near you and meet people in your local community who share your interests.
Long Road Ahead, the third episode in Telltales critically acclaimed and commercially successful The Walking Dead adventure series, is out now…
The Walking Dead TV series has quickly turned itself into what is becoming a cult classic, with a huge following of fans that cant get enough of the rapidly changing storyline and loss of and introduction of new characters into the zombie tale. ...
Our dog walking services lead to a much happier and more well-behaved dog. Almost all dogs crave getting outside and exercising, instead of sitting inside.
This report aims to help states and local communities set goals, plan strategies, and evaluate results of their bicycling and walking initiatives.
"Identification of the cystic fibrosis gene: chromosome walking and jumping". Science. 8 September 1989. Retrieved 30 October ...
... it provides the library of a genomic format that chromosome walking requires.[citation needed] Yeast artificial chromosome or ... Chromosome walking can be used for the purpose of cloning a gene. It does this by using the known gene's markers that are ... Chromosome walking is also useful when it comes to filling in the gaps that may be present in genomes by locating clones that ... This is why Vectorette PCR is one of the methods that can be used to create this library for chromosome walking to occur. ...
This technique, called chromosome walking, can be exploited to sequence entire chromosomes. Whole genome shotgun sequencing is ... P1 artificial chromosomes (PACs) have features of both P1 vectors and Bacterial Artificial Chromosomes (BACs). Similar to P1 ... Yeast artificial chromosomes (YACs) are linear DNA molecules containing the necessary features of an authentic yeast chromosome ... The λ chromosome is 48.5kb long and can carry inserts up to 25kb. These inserts replace non-essential viral sequences in the λ ...
Chromosome Walk, the SIB virtual exhibition. Official website Swiss Bioinformatics Resource Portal (Expasy). ...
Meselson, M.; Weigle, J. J. (1961-06-15). "Chromosome brekage accompanying genetic recombination in bacteriophage". Proceedings ...
Meselson, M.; Weigle, J. (1961). "Chromosome Breakage Accompanying Genetic Recombination in Bacteriophage". Proceedings of the ... normally used by the phage to insert itself into the chromosome of a host cell. Subsequently, variations of the experiment by ...
... chromosome walking and jumping". Science. 245 (4922): 1059-65. Bibcode:1989Sci...245.1059R. doi:10.1126/science.2772657. PMID ...
September 1989). "Identification of the cystic fibrosis gene: chromosome walking and jumping". Science. 245 (4922): 1059-65. ... The CFTR gene is located on the long arm of chromosome 7, at position q31.2, and ultimately codes for a sequence of 1,480 amino ... The gene that encodes the human CFTR protein is found on chromosome 7, on the long arm at position q31.2. from base pair ... ΔF508 is present on at least one copy of chromosome 7 in approximately one in 30 Caucasians. Presence of the mutation on both ...
Afterward, chromosome walking and jumping techniques were used to identify the gene and sequence it. Forward genetics can work ... chromosome walking and jumping". Science. 245 (4922): 1059-65. Bibcode:1989Sci...245.1059R. doi:10.1126/science.2772657. PMID ... process and much of the work went into mapping and cloning the gene through association studies and chromosome walking. Despite ... Genetic-linkage studies were able to map the disease loci in cystic fibrosis to chromosome 7 by using protein markers. ...
They then used a combination of chromosome walking and chromosome hopping or jumping to locate the CF gene, which they named ... 1989-09-08). "Identification of the cystic fibrosis gene: chromosome walking and jumping". Science. 245 (4922): 1059-1065. doi: ... each chromosome a child inherits is a mix of the both of that parent's copies of that chromosome. Markers would only be ... Due to the phenomenon of recombination, whereby parts of chromosomes swap homologous segments during germ cell development, ...
Using protein markers, gene-linkage studies were able to map the mutation to chromosome 7. Chromosome walking and chromosome ... September 1989). "Identification of the cystic fibrosis gene: chromosome walking and jumping". Science. 245 (4922): 1059-1065. ... The CFTR gene, found at the q31.2 locus of chromosome 7, is 230,000 base pairs long, and creates a protein that is 1,480 amino ... More specifically, the location is between base pair 117,120,016 and 117,308,718 on the long arm of chromosome 7, region 3, ...
This technique is an extension of "chromosome walking" that allows for larger "steps" along the chromosome. If we desire to ... In the early days, chromosome walking from genetically linked DNA markers was used to identify and clone disease genes. However ... Chromosome jumping (or chromosome hopping) was first described in 1984 by Collins and Weissman. At the time, cloning techniques ... 8 September 1989). "Identification of the cystic fibrosis gene: chromosome walking and jumping". Science. 245 (4922): 1059-1065 ...
... chromosome walking and jumping". Science. 245 (4922): 1059-65. Bibcode:1989Sci...245.1059R. PMID 2772657. doi:10.1126/science. ... Cloning of the cDNA for a human homologue of the Drosophila white gene and mapping to chromosome 21q22.3". Am J Hum Genet. 59 ( ...
Google Scholar citation The treasures of chromosome 7. Autumn 2001. The University of Toronto Magazine Walking the jungles and ... Chromosome 7: DNA Sequence and Biology. 2003. Science 300, 767-772. Google Scholar citation Minassian et al. Mutations in a ... Earlier (1988-2003) with Lap-chee Tsui, he led studies of human chromosome 7, in particular in the mapping phase of the Human ... Structural variation of chromosomes in autism spectrum disorder. 2008. American Journal of Human Genetics 82, 477-88. Google ...
If the chromosome walk proceeds through the mutant allele, the new polymorphisms will start to show increase in recombination ... This process produces a contig map of the locus and is known as chromosome walking. With the completion of genome sequencing ... Suppressor mutations can be described as second mutations at a site on the chromosome distinct from the mutation under study, ... By the classical genetics approach, a researcher would then locate (map) the gene on its chromosome by crossbreeding with ...
They are-with accompanying Y-chromosome locations-U5 (rs2178500), L149 (8486380) and L31 (also called S149) (rs35617575.. ... In 2009-10, Family Tree DNA's Walk through the Y Project, sequencing certain Y-chromosome segments, provided a number of new G ... The mutation is found on the Y chromosome at 10595022 and is a change from G to C. G-L30 (also G-PF3267, G-S126 or G-U8; G2a2b ... Its chromosome location listed as 21653414. G2a was found in medieval remains in a 7th- century CE high-status tomb in ...
Two principal methods are used for this: primer walking (or "chromosome walking") which progresses through the entire strand ... one of these clones can then be sequenced to yield a new probe and the process repeated in a method called chromosome walking. ... From this map, a minimal number of fragments that cover the entire chromosome are selected for sequencing.[14] In this way, the ... Bacterial artificial chromosomes) followed by sequencing of the vector. ...
... automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking". Genomics. 25 ( ... Universal Fast Walking: for genome walking and genetic fingerprinting using a more specific 'two-sided' PCR than conventional ' ... and allows unidirectional genome walking from known into unknown regions of the chromosome. Solid Phase PCR: encompasses ... Boehnke M, Arnheim N, Li H, Collins FS (July 1989). "Fine-structure genetic mapping of human chromosomes using the polymerase ...
... automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking". Genomics. 25 ( ... This method is deployed for DNA sequencing, genome walking, and DNA footprinting. A related technique is amplified fragment ...
... automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking". Genomics. 25 ( ... Universal Fast Walking: for genome walking and genetic fingerprinting using a more specific 'two-sided' PCR than conventional ' ... and allows unidirectional genome walking from known into unknown regions of the chromosome.[63] ... Shyamala, V.; Ferro-Luzzi, Ames G. (1993). Single Specific Primer-Polymerase Chain Reaction (SSP-PCR) and Genome Walking. ...
... organization of genes and repeats in the large cereal genomes and implications for the isolation of genes by chromosome walking ... Different large-insert libraries like BACs, P1 artificial chromosomes (PAC), yeast artificial chromosome (YAC) and ... The most common type of large-insert clone is the bacterial artificial chromosome (BAC). With BAC, the genome is first split ... Next, using the map from the first step the contigs are assembled back into the chromosomes. The first complete plant genome ...
... chromosome walking and jumping". Science 245 (4922): 1059-65. Bibcode 1989Sci...245.1059R. . PMID 2772657. ...
"Diploid/Polyploid Syntenic Shuttle Mapping and Haplotype-Specific Chromosome Walking Toward a Rust Resistance Gene (Bru1) in ... Each chromosome pair derived from the Triticum urartu parent is homoeologous to the opposite chromosome pair derived from the ... Although chromosomes are shattered into many fragments, complete chromosomes can be regenerated by making use of overlapping ... Durum wheat contains a hybrid genome with two sets of chromosomes derived from Triticum urartu and two sets of chromosomes ...
If the position of the gene can be determined using molecular markers then chromosome walking is one way to isolate the correct ...
Primer walking (or "chromosome walking") progresses through the entire strand piece by piece, whereas shotgun sequencing is a ... one of these clones can then be sequenced to yield a new probe and the process repeated in a method called chromosome walking. ... From this map, a minimal number of fragments that cover the entire chromosome are selected for sequencing. In this way, the ... If the gap is large (>20kb) then the large fragment is cloned in special vectors such as bacterial artificial chromosomes (BAC ...
They then used a combination of chromosome walking and chromosome hopping or jumping to locate the CF gene, which they named ... each chromosome a child inherits is a mix of the both of that parent's copies of that chromosome. Markers would only be ... In the late 1990s she began studying chromosome structure and function. She has investigated genome instability and made ... Due to the phenomenon of recombination, whereby parts of chromosomes swap homologous segments during germ cell development, ...
... to emphasize the contrast with an older and much more time-consuming method of copying DNA fragments called chromosome walking ... who agreed to collaborate with the Toronto team and share his chromosome-jumping technique. The gene was identified in June ... "A novel gene containing a trinucleotide repeat that is expanded and unstable on Huntington's disease chromosomes" (PDF). Cell. ... The method described was named chromosome jumping, ...
It is believed to have originated by chromosome doubling in a hybrid between walking fern and mountain spleenwort (Asplenium ... In 1956, C. V. Morton pointed out that, as A. pinnatifidum had been shown to arise from hybridization between walking fern and ... It is intermediate in morphology between the parent species: while its leaf blades are long and tapering like that of walking ... In 1953, he made chromosome counts of A. × trudellii, which had been classified by some simply as a variety of A. pinnatifidum ...
... chromosome - chromosome walking - cilium - circular dichroism - - cis face - Citric acid - Citric acid cycle - cladistics - ... Y chromosome - Yeast - Zymology - Contents: Top 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z List of biochemists for ... Philadelphia chromosome - phospholipid - phospholipid bilayer - phosphopeptide - phosphoprotein - phosphorus - phosphorylation ...
In search of the genetic footprints of Sumerians: a survey of Y-chromosome and mtDNA variation in the Marsh Arabs of Iraq - - ... In the later twentieth century a third main occupation entered Marsh Arab life; the weaving of reed mats on a commercial scale ...
It has been argued that walking into a toilet separated by sex requires people to self-separate and that some transgender ... chromosomes and anatomy' at birth.[32] ...
These tumors show a high frequency of co-deletions of the p and q arms of chromosome 1 and chromosome 19 respectively (1p19q co ... Other symptoms may include difficulty walking, speaking or with sensations.[1][3] As the disease progresses, unconsciousness ...
adult walking behavior. • Перистальтика. • cellular response to catecholamine stimulus. • adenylate cyclase-activating dopamine ... 1990). "Human dopamine D1 receptor encoded by an intronless gene on chromosome 5". Nature. 347 (6288): 80-83. DOI:10.1038/ ...
Most have delayed development of mental abilities and motor skills such as sitting and walking. Other neurological problems can ... "A testis-specific form of the human pyruvate dehydrogenase E1 alpha subunit is coded for by an intronless gene on chromosome 4 ... include intellectual disability, seizures, weak muscle tone (hypotonia), poor coordination, and difficulty walking. Some ...
The IDS gene is located on the X chromosome. Females have two X chromosomes, one inherited from each parent, whereas males have ... On December 13, 2005, Andrew Wragg walked out of Lewes Crown Court a free man after a jury determined that he did not murder ... Alternatively, during egg and sperm formation, a mutation can develop in the IDS gene on his X chromosome. In this second case ... one X chromosome that they inherit from their mother and one Y chromosome that they inherit from their father. If a male has an ...
The LDL receptor gene is located on the short arm of chromosome 19 (19p13.1-13.3).[7] It comprises 18 exons and spans 45 kb, ... this can cause pain in the calf muscles during walking that resolves with rest (intermittent claudication) and problems due to ... Its gene is located on the second chromosome (2p24-p23) and is between 21.08 and 21.12 Mb long. FH is often associated with the ... The gene is located on the first chromosome (1p34.1-p32) and encodes a 666 amino acid protein that is expressed in the liver. ...
... but lost his nerve at the last minute and walked out of the classroom, never to return. On his long walk home, he came up with ... Haldane was also the first to construct human gene maps for haemophilia and colour blindness on the X chromosome and he was one ... He won the Nobel Prize in Physiology or Medicine in 1933 for discoveries relating the role the chromosome plays in heredity.[ ... swimming and walking were among the few things they could do together.. ...
He then walked to the next town where he was arrested for being an Aboriginal vagrant and sent to the reserve there. During ... 2015). "Antiquity and diversity of aboriginal Australian Y-chromosomes". American Journal of Physical Anthropology. 159 (3): ... "Aboriginal Heritage walk: Ku-ring-gai Chase National Park". NSW National Parks and Wildlife Service. Retrieved 29 January 2017. ... "Collaborating for Indigenous Rights: Wave Hill walk off, 1966-1967". National Museum of Australia. Archived from the original ...
In the 2003 PBS programme African American Lives, Bishop T.D. Jakes had his DNA analyzed; his Y chromosome showed[dubious - ... "Migration and business: Weaving the world together". The Economist. 2011-11-19. Retrieved 2015-04-12.. ...
SMN1 is located in a telomeric region of human chromosome 5 and also contains SMN2 in a centromeric region. SMN1 and SMN2 are ... As the disease progresses most patients are unable to walk or use their arms and eventually develop difficulty speaking, ... The SOD1 gene on chromosome 21 that codes for the superoxide dismutase protein is associated with 2% of cases and is believed ... Todd TW, Petrucelli L (August 2016). "Insights into the pathogenic mechanisms of Chromosome 9 open reading frame 72 (C9orf72) ...
A variant in the gene for tau protein called the H1 haplotype, located on chromosome 17, has been linked to PSP.[4] Nearly all ... Due to the progressive nature of this disease, all individuals eventually lose their ability to walk and will need to progress ... The initial symptoms in two-thirds of cases are loss of balance, lunging forward when mobilizing, fast walking, bumping into ...
Methylation of cytosines can also persist from the germ line of one of the parents into the zygote, marking the chromosome as ... In one example, mice were conditioned to fear a strong scent, acetophenone, by accompanying the smell with an electric shock. ... In 2008, a consensus definition of the epigenetic trait, "stably heritable phenotype resulting from changes in a chromosome ... Specific epigenetic processes include paramutation, bookmarking, imprinting, gene silencing, X chromosome inactivation, ...
European Chromosome 11 Network - Support group for patients with chromosome 11 disorders, their families and relatives ... standing and walking difficult. Most children eventually start speaking, but in cases with severe intellectual disability ... Ring chromosome 11- in this case genetic material from both long and short arm of the chromosome get deleted, and the remaining ... The affected children have deletions on chromosome 11 as well as some extra genetic material from another chromosome.[13] ...
These ten chromosomes form five unique pairs of XY in males and XX in females, i.e. males are X1Y1X2Y2X3Y3X4Y4X5Y5.[76] One of ... The webbing on the feet is more significant on the front feet and is folded back when walking on land. The elongated snout and ... "In the platypus a meiotic chain of ten sex chromosomes shares genes with the bird Z and mammal X chromosomes". Nature. 432 ( ... the X chromosomes of the platypus has great homology to the bird Z chromosome.[77] The platypus genome also has both reptilian ...
While twisting, the magnetic tweezers also allow stretching the DNA molecule. This way, torsion extension curves may be ... as well as interactions with topoisomerases or histones in chromosomes .[17] ...
Cytogenetic testing is the study of chromosomes to detect gains or losses of chromosomes or chromosome segments using ... Individuals with these toe abnormalities walk normally and can wear normal footwear. In more severe cases, frequent surgeries ... The mouse TWIST gene is located on chromosome 12 in mice, which corresponds to the short arm of chromosome 7 in humans. With ... Occasionally, individuals with SCS have a chromosome translocation, inversion, or ring chromosome 7 involving 7p21 resulting in ...
For example, a three-year-old child who is not able to walk has a disability because a normal three-year-old can walk ... "A gene for ataxic cerebral palsy maps to chromosome 9p12-q12". European Journal of Human Genetics. 8 (4): 267-272. doi:10.1038/ ... 886 Much of childhood therapy is aimed at improving gait and walking. Approximately 60% of people with CP are able to walk ... or more adults with cerebral palsy who can walk experience increasing difficulties walking with age.[134] Chronic disease risk ...
... situated on avian chromosome 18) in the hooded crow, which imparts the lighter plumage colouration on its torso.[8] Thus the ... whose geographic distributions across Europe are illustrated in the accompanying diagram. It is believed that this distribution ...
In some species (especially herbivores living on plains) the young can walk and even run within an hour of birth. ...
Listed here are the human Y-chromosome DNA haplogroups in Arabia (Yemen,[18] Oman,[19] Qatar,[20] Kuwait,[21] Saudi Arabia[22] ... Traditional handicrafts such as carpet-weaving are found in rural areas of Arabia.[citation needed] ... Y-chromosome haplogroup frequencies (%) in the studied populations. doi:10.1371/journal.pone.0056775.s006 (XLS) ... Haplogroup J is the most abundant component in the Arabian peninsula, embracing more than 50% of its Y-chromosomes. ...
DiGeorge syndrome is a genetic disorder caused by the deletion of a small section of chromosome 22. This results in a midline ... Lymphatic vessels travel only away from the thymus, accompanying the arteries and veins. These drain into the brachiocephalic, ... The gene that codes for the enzyme called ADA (adenine deaminase), is located on chromosomes 20. The HIV virus causes an ...
... s have 21 pairs of chromosomes (2n=42).[106] The genome of live whales can be examined by recovering shed skin.[107] ... the bull sperm whale when accompanying pods of female sperm whales and their calves as such may be reportedly unable to ...
The knuckle-walking of the common ancestor gave way to bipedalism, which is more efficient over longer distances. A larger ... Markers in these Y chromosomes can be linked to the Basques, and he suggests that the British and Irish descend from a refuge ... Through the Y chromosome and mitochondrial DNA, Wade gives evidence to suggest that the ancestral population was no more than ... Wade also discusses the origin of Jews; they descend from the Middle East through their Y chromosomes, but their mitochondrial ...
This was about 1.25 mi (2 km) from his home so he could walk there and back, by Park Avenue South and Abington Park Crescent, ... the other major component of chromosomes, as a candidate genetic molecule. In the 1944 Avery-MacLeod-McCarty experiment, Oswald ...
"Horsey-aeology, Binary Black Holes, Tracking Red Tides, Fish Re-evolution, Walk Like a Man, Fact or Fiction". Quirks and Quarks ... there is very little variability in the Y-chromosome between breeds.[212] Following domestication of the horse, due to the ... Stallions may be shown in most youth classes, except for 8 and under walk-trot: 2008 USEF Arabian, Half-Arabian and Anglo- ...
When walking, the dingo's rear foot steps in line with the front foot,[7] and these do not possess dewclaws.[31] ... The wolf-like canids are a group of large carnivores that are genetically closely related because their chromosomes number 78, ...
"Bird-like sex chromosomes of platypus imply recent origin of mammal sex chromosomes", Genome Res. 2008 June; 18(6): 965-973 ... You must provide copyright attribution in the edit summary accompanying your translation by providing an interlanguage link to ... as the fact that monotremes possess 5 pairs of sex chromosomes and that one of the X chromosomes resembles the Z chromosome of ... "Origins and functional evolution of Y chromosomes across mammals". Nature. 508 (7497): 488-493. doi:10.1038/nature13151.. ...
The circular chromosome contains 1,830,137 bases and its publication in the journal Science[33] marked the first published use ... De novo translates from Latin as "from the beginning". Gaps in the assembled sequence may be filled by primer walking. The ... As a consequence, many sequences may not be assigned to particular chromosomes. The production of raw sequence data is only the ... Large-scale sequencing often aims at sequencing very long DNA pieces, such as whole chromosomes, although large-scale ...
What is chromosome walking? Meaning of chromosome walking medical term. What does chromosome walking mean? ... Looking for online definition of chromosome walking in the Medical Dictionary? chromosome walking explanation free. ... This makes it possible to walk along the chromosome from one gene to the next, to reach a desired LOCUS. Chromosome walking ... chromosome walking. Also found in: Encyclopedia. chro·mo·some walk·ing. sequential isolation of overlapping sequences of DNA ( ...
Identification of the cystic fibrosis gene: chromosome walking and jumping. By JM Rommens, MC Iannuzzi, B Kerem, ML Drumm, G ... Identification of the cystic fibrosis gene: chromosome walking and jumping. By JM Rommens, MC Iannuzzi, B Kerem, ML Drumm, G ... Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than ... Identification of the cystic fibrosis gene: chromosome walking and jumping Message Subject. (Your Name) has forwarded a page to ...
Walking on the chromosome: Drosophila and the molecularization of development. and the molecularization ...
Walking Along Chromosomes: Visualizing in situ Chromosomal Structure with Single Molecule Localization Microscopy. Fluorescence ... Walking Along Chromosomes: Visualizing in situ Chromosomal Structure with Single Molecule Localization Microscopy ... Walking Along Chromosomes: Visualizing in situ Chromosomal Structure with Single Molecule Localization Microscopy ... By incorporating microfluidics and sequential labelling strategies can be employed to "walk" along chromosomes to visualize ...
Additional trisomies amongst patients with chronic lymphocytic leukemia carrying trisomy 12: the accompanying chromosome makes ... Additional trisomies amongst patients with chronic lymphocytic leukemia carrying trisomy 12: the accompanying chromosome makes ... Additional trisomies amongst patients with chronic lymphocytic leukemia carrying trisomy 12: the accompanying chromosome makes ... the accompanying chromosome makes a difference. Haematologia, 1-21. (doi:10.3324/haematol.2015.140202). (PMID:27102498) ...
Chromosome walking at the fertility restorer locus Rf1 in sunflower (Helianthus annuus L.). Rostock : Universität , 2009 ...
... is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome. As ... The method can be used to sequence entire chromosomes (thus, chromosome walking). A different method with the same purpose ... Basically, chromosome walking works as follows: * A primer that matches the beginning of the DNA to sequence is used to ... That way, the short part of the long DNA that is sequenced keeps "walking" along the sequence. ...
Chromosome Walking.. A contig of bacterial artificial chromosome (BAC) genomic DNA clones derived from the Texas A & M ... 1992) Chromosome Walking in Arabidopsis thaliana Using Yeast Artificial Chromosomes. (World Scientific, Teaneck, NJ).. ... A) Genetic map of a region of chromosome 1 between the genes ETR1 and APETELA 1 (AP1). Genetic and molecular markers are listed ... Generation of a Collection of Plants Carrying Recombined Polymorphic Chromosome 1S with Recombination Breakpoints in Proximity ...
SIB Swiss Institute of Bioinformatics. CMU, 1 Michel Servet. 1211 Geneva 4. Tel: +41 22 379 50 50. [email protected] www.sib.swiss. ...
chromosome scaffolding. H2AFZ: This is one of the numerous genes that code for histone proteins. The characteristic ... Chromosome 4 contains vast regions, known as deserts, in which there are no genes at all. ... architecture of a chromosome is due to the way DNA is wound around histones. ...
"50 Mums , 50 Kids , 1 Extra Chromosome" - Walking around America. On 22/09/2018. 22/09/2018. By Peters ponderingIn beauty, ... "50 Mums , 50 Kids , 1 Extra Chromosome" - Walking around America" * Sue W ...
Chromosome Walking. Pollen from sin1-1 (La-er) was crossed to wild-type Col stigma and F1 were self-crossed. DNA samples from F ... 6). Of these, T15B3.60 (At3g43920) and T17B22.28 (At3g03300) are on chromosome III and F5024.210 (At5g20320) is on chromosome V ... and F7I23 are bacterial artificial chromosomes (BACs); and pJT12 is a plasmid subclone of Arabidopsis chromosome I. The ... Sampling over 700 F2chromosomes yielded no crossover between sin1 and nga59.sin1 was mapped to the Arabidopsis yeast ( ...
... from the cystic fibrosis region on the long arm of human chromosome 7, band q31 (Rommens et al. 1989). Chromosome walking is a ... whose article detailing the experiment was published in 1989. Chromosome walking and jumping and complementary DNA ... What Is Chromosome Walking And Jumping And Complementary DNA Hybridization?. 922 Words , 4 Pages. Previous Research The study ... What Is Chromosome Walking And Jumping And Complementary DNA Hybridization?. 922 Words , 4 Pages ...
Chromosome A chromosome is a structure that occurs within cells and that contains the cells genetic material. That genetic ... chromosome walking A technique, used in constructing a physical map, for selecting contiguous overlapping clones from a DNA ... Lilies have 24 chromosomes, earthworms have 36 chromosomes, chimps have 48 chromosomes, and horses have 64 chromosomes. The ... lilies have 24 chromosomes, earthworms have 36 chromosomes, chimps have 48 chromosomes, and horses have 64 chromosomes. ...
Primer walking synonyms, Primer walking pronunciation, Primer walking translation, English dictionary definition of Primer ... walking. n. 1. An elementary textbook for teaching children to read. 2. A book that covers the basic elements of a subject. n. ... Related to Primer walking: Chromosome walking prim·er 1. (prĭm′ər). n.. 1. An elementary textbook for teaching children to read ... Primer walking - definition of Primer walking by The Free Dictionary https://www.thefreedictionary.com/Primer+walking ...
... verticillus were determined by chromosome walking. From the results of Real time fluorescence quantitative PCR (RT-qPCR) and ... Figure S1. Detail schematic diagram of chromosome walking by SiteFinding PCR and nested PCR. Figure S2. SDS-PAGE profile of 6 ... Tan G, Gao Y, Shi M, Zhang X, He S, Chen Z, An C. SiteFinding-PCR: a simple and efficient PCR method for chromosome walking. ... To extract genomic DNA used for chromosome walking, the mature spores were suspended in Tryptic Soy Broth (TSB) medium (BD ...
Molecular genetic analysis: Chromosome walking; gene tagging; RFLP analysis. In: Methods in Plant Biochemistry 10b. Academic ... Detection of a region of Arabidopsis chromosome 5 between the genetic markers ms1 and ttg, which undergoes a high frequency of ... Molecular physical mapping of the lu-ttg region of Arabidopsis chromosome 5 Weeds World. 4, Oct-21 ... RFLP; physical mapping of an 8.9cM region of Arabidopsis chromosome 5; which includes Ms1; an essential gene for pollen ...
Mapping human chromosomes by walking with sequence-tagged sites from end fragments of yeast artificial chromosome inserts. In: ... Mapping human chromosomes by walking with sequence-tagged sites from end fragments of yeast artificial chromosome inserts. / ... T1 - Mapping human chromosomes by walking with sequence-tagged sites from end fragments of yeast artificial chromosome inserts ... Mapping human chromosomes by walking with sequence-tagged sites from end fragments of yeast artificial chromosome inserts. ...
Chromosome Walking A method of determining the DNA sequence of a chromosome by extending out from a known region, designing a ... Chromosome Walking. CIP. To CIP. Cis. Clean Up. Clone (noun) To clone (verb). Coding sequence. Coding strand. Codon. Codon ... Bacterial Artificial Chromosome. A circular DNA molecule that is replicated and segregated by the same mechanisms as the ... For instance, in E. coli, the protein DnaA recognizes DnaA boxes in the OriC region of the chromosome. ...
A cosmid vector for systematic chromosome walking.. Cross SH, Little PF.. Gene. 1986;49(1):9-22. ... Genetic and physical mapping of a gene encoding a methyl CpG binding protein, Mecp2, to the mouse X chromosome. ... CpG island libraries from human chromosomes 18 and 22: landmarks for novel genes. ...
... it provides the library of a genomic format that chromosome walking requires.[citation needed] Yeast artificial chromosome or ... Chromosome walking can be used for the purpose of cloning a gene. It does this by using the known genes markers that are ... Chromosome walking is also useful when it comes to filling in the gaps that may be present in genomes by locating clones that ... This is why Vectorette PCR is one of the methods that can be used to create this library for chromosome walking to occur. ...
"Identification of the cystic fibrosis gene: chromosome walking and jumping". Science. 8 September 1989. Retrieved 30 October ...
Studies Of Gryllid Chromosomes II. Chromosomal Polymorphisms In Pteronemobius Taprobanensis (Walk.), And Chromosome Morphology ... Studies Of Gryllid Chromosomes II. Chromosomal Polymorphisms In Pteronemobius Taprobanensis (Walk.), And Chromosome Morphology ...
30 Cloning Genes of Arabidopsis thaliana by Chromosome Walking Jeffrey Leung, Jéréme Giraudat ... Chromosome Landing Using an AFLPTWased Strategy Ann Van Gysel, Gerda Cnops, Peter Breyne, Marc Van Montagu, Maria Teresa ...
2005) are now ready for chromosome walking. BAC-DNA samples including known genes will be provided from the DNA bank of the ... Sahara, K., F. Marec and W. Traut, 1999 TTAGG telomeric repeats in chromosomes of some insects and other arthropods. Chromosome ... The chromosome corresponding to LG24 is the second largest chromosome, which also harbors the largest autosomal heterochromatic ... Correlation of genetic and physical lengths of each chromosome. The average relative size of all chromosomes is defined as 1. ...
Identification of the cystic fibrosis gene: chromosome walking and jumping. Science. 1989 Sep 8. 245(4922):1059-65. [Medline]. ...
Identification of the cystic fibrosis gene: chromosome walking and jumping. Science. 1989 Sep 8. 245(4922):1059-65. [Medline]. ...
BAC contig was made by chromosome walking (see SI Methods ). Shotgun sequencing of the BACs was carried out by the standard ... clones by means of chromosome walking. The contig containing vrs1 is composed of 518,343 bp. Annotation showed three predicted ... Open circles indicate markers uniquely assigned to chromosome 2H, of which genetically mapped markers are connected with the ... 1 J). The candidate genomic region was covered completely with six bacterial artificial chromosome (BAC) ...
Hadfield, "Chromosome Walking," Focus, 5:1-5 (1983). Hochhauser, "Bringing Biotechnology to Market," High Technology, Feb.:55- ... Kohara et al., "The Physical Map of the Whole E. coli Chromosome: Application of a New Strategy for Rapid Analysis and Sorting ... Burke et al., "Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors," Science, 236: ... Nasmyth and Sulston, "High-altitude Walking with YACs" Nature, 328:380-381 (1987). Ohtsuka et al., "An Alternative Approach to ...
Initial localization of the gene garnet (g) to a 6.5-kb EcoRI fragment of the chromosome walk was made through the ... Mitotic chromosome defects in mus101lcd neuroblasts. (A) Wild-type XX metaphase. (B) Chromosomes from a mus101lcd/Y larva ... Here we describe the cloning of mus101, achieved through a strategy of chromosome walking and mutation mapping in region 12B. ... Subcloned restriction fragments mentioned in the text are labeled (e.g., B15). EDGP cosmids that comprise the chromosome walk ...
  • The scientists identified two marker genes, or sites of known traits, on the chromosomes of the disease-resistant tomato plant. (thefreedictionary.com)
  • Chromosome 4 contains vast regions, known as deserts, in which there are no genes at all. (chromosomewalk.ch)
  • At one point in the cell's life, its chromosomes become untangled and open up to expose their genes. (encyclopedia.com)
  • Genes Chromosomes Cancer 58 , 427-436 (2019). (nature.com)
  • These vectors should be valuable for isolation and analysis of plant genes, using transformation, library screening, and chromosome-walking approaches. (caltech.edu)
  • In Y. pestis , a 102-kb chromosomal fragment ( pgm locus) that carries genes involved in iron acquisition and colony pigmentation can be deleted en bloc. (asm.org)
  • It also carries a single copy of four different sequences repeated elsewhere in the chromosome (RS.3, RS.4, IS 1400 [ 5 ], and IS 1328 [ 41 ]) and genes involved in siderophore-mediated iron acquisition: the yersiniabactin biosynthetic genes irp2 and, probably, irp1 ( 5 ) and the fyuA locus, which codes for the yersiniabactin receptor ( 42 ). (asm.org)
  • We want to start with a human Y," he says, referring to the male sex chromosome, which he explains has the fewest genes of a person's 23 chromo-somes and is thus easier to build. (wired.com)
  • The relocalisation of some genes to positions outside chromosome territories, and the visible decondensation or unfolding of interphase chromatin, are two striking facets of nuclear reorganisation linked to gene activation that have been assumed to be related to each other. (biologists.org)
  • Genes on B chromosomes: Old questions revisited with new tools. (ipk-gatersleben.de)
  • A genome map describes the order of genes or other markers and the spacing between them on each chromosome. (lsu.edu)
  • Over the last five years, scientists have built bacterial chromosomes and viral DNA, but this is the first report of an entire eukaryotic chromosome, the threadlike structure that carries genes in the nucleus of all plant and animal cells, built from scratch. (newswise.com)
  • a technique for mapping chromosomes that involves the sequential isolation of clones carrying overlapping NUCLEOTIDE SEQUENCES . (thefreedictionary.com)
  • Chromosome walking and jumping and complementary DNA hybridization were used to isolate DNA sequences, encompassing more than 500,000 base pairs, from the cystic fibrosis region on the long arm of human chromosome 7. (sciencemag.org)
  • To mine the gene information relevant to GlcNAc metabolism, the DNA sequences of dasR - dasA - dasBCD - nagB and nagKA in S. verticillus were determined by chromosome walking. (springer.com)
  • A second-generation linkage map was constructed for the silkworm, Bombyx mori , focusing on mapping Bombyx sequences appearing in public nucleotide databases and bacterial artificial chromosome (BAC) contigs. (genetics.org)
  • Thus, sequences not reachable by chromosome walking can be sequenced. (wikipedia.org)
  • Chromosome walking can be used from the new jump position (in either direction) to look for gene-like sequences, or additional jumps can be used to progress further along the chromosome. (wikipedia.org)
  • strain CN41 by using homology-based cloning followed by SiteFinding PCR ( 17 ), a chromosome walking method to amplify the flanking sequences. (asm.org)
  • A simple and rapid procedure to isolate clones carrying sequences from a specific region of the polytene chromosome of Drosophila is demonstrated. (nih.gov)
  • Chromosome walking is used in genome sequencing projects, see CLONE CONTIG SEQUENCING METHOD . (thefreedictionary.com)
  • 2003) showed that the availability of the extensive rice genome sequence data could be used to generate markers for saturation mapping to expedite chromosome walking to a gene of interest in a related species. (thefreedictionary.com)
  • A bacterial artificial chromosome (BAC)-based approach for integration of genome information has a definite advantage for facilitating further functional analysis. (genetics.org)
  • Here, we describe iFISH, an open-source repository currently comprising 380 DNA FISH probes targeting multiple loci on the human autosomes and chromosome X, as well as a genome-wide database of optimally designed oligonucleotides and a freely accessible web interface ( http://ifish4u.org ) that can be used to design DNA FISH probes. (nature.com)
  • In the past decade, DNA FISH techniques have become increasingly popular among genome biologists, thanks to the fact that they enable direct observation of the three-dimentional (3D) genome architecture in a manner that is complementary to chromosome conformation capture methods, such as Hi-C 1 . (nature.com)
  • pahge, cosmid, BAC or yeast artificial chromosome vectors can be used to construct genomic libraries, the choice depending on the genome size. (powershow.com)
  • Chromosome organization is crucial for genome function. (rice.edu)
  • Chromosome jumping allows more rapid movement through the genome compared to other techniques, such as chromosome walking, and can be used to generate genomic markers with known chromosomal locations. (wikipedia.org)
  • His plan is to design and build long chains of human DNA, not solely by cutting and pasting small fixes-a now-routine practice, thanks to recent technologies like Crispr that let scientists edit DNA cheaply and easily-but by rewriting critical stretches of chromosomes that can then be stitched together with a naturally occurring genome. (wired.com)
  • He and his team want to use the Y chromosome sequence from an actual person's genome: mine. (wired.com)
  • A set of cytogenetic markers allows the precise identification of all A-genome chromosomes in diploid and polyploid wheat. (ipk-gatersleben.de)
  • A primary goal of the Human Genome Project is to make a series of descriptive diagrams maps of each human chromosome at increasingly finer resolutions. (lsu.edu)
  • Chromosomal Polymorphisms In Pteronemobius Taprobanensis (Walk. (iitkgp.ac.in)
  • Chromosomal abnormalities, seen on a karyotype, accompanying a disease e.g. (studystack.com)
  • We showed that HD-FISH probes can be used to localize individual genomic loci at sub-diffraction limit resolution, as well as to visualize chromosomal territories as discrete clouds of fluorescence spots-an approach which we named 'chromosome spotting' 2 , that allows to quantitatively assess various structural properties of chromosomes. (nature.com)
  • At the same time, another group reported Oligopaint FISH 3 , a versatile method to produce DNA FISH oligo probes, named Oligopaints, which can be used to visualize entire chromosomes or sub-chromosomal regions. (nature.com)
  • A woman seeking genetic counseling, was found to be heterozygous for a chromosomal rearrangement between the second and third chromosomes. (columbia.edu)
  • The method can be used to sequence entire chromosomes (thus, chromosome walking ). (infomutt.com)
  • Sequence-tagged sites (STSs) derived from end fragments of chromosome-specific yeast artificial chromosomes (YACs) can facilitate the assembly of an overlapping YAC/STS map. (wustl.edu)
  • Cloning of Large Segments of Exogenous DNA into Yeast by Means of Artificial Chromosome Vectors," Science, 236:806-812 (1987). (freepatentsonline.com)
  • Newswise - An international team of scientists led by Jef Boeke, PhD, director of NYU Langone Medical Center's Institute for Systems Genetics, has synthesized the first functional chromosome in yeast, an important step in the emerging field of synthetic biology, designing microorganisms to produce novel medicines, raw materials for food, and biofuels. (newswise.com)
  • We have shown that yeast cells carrying this synthetic chromosome are remarkably normal. (newswise.com)
  • In this week's issue of Science online March 27, the team reports how, using computer-aided design, they built a fully functioning chromosome, which they call synIII, and successfully incorporated it into brewer's yeast, known scientifically as Saccharomyces cerevisiae. (newswise.com)
  • We have made over 50,000 changes to the DNA code in the chromosome and our yeast still live. (newswise.com)
  • It shows that our synthetic chromosome is hardy, and it endows the yeast with new properties. (newswise.com)
  • Yeast chromosome III was selected for synthesis because it is among the smallest of the 16 yeast chromosomes and controls how yeast cells mate and undergo genetic change. (newswise.com)
  • Bacterial Artificial Chromosome. (sigmaaldrich.com)
  • Their GC content is usually different from that of the rest of the host chromosome, suggesting that they originate from horizontal transfer between different bacterial genera ( 24 ). (asm.org)
  • Chromosome walking is a method in genetics for identifying and sequencing long parts of a DNA strand, e.g., a chromosome . (infomutt.com)
  • You can see that APC and apc are located corresponding to each other in the long arm of chromosome #5. (hubpages.com)
  • The cystic fibrosis transmembrane regulator (CFTR) gene is a large, ∼250 kb, gene that is located on the long arm of chromosome 7. (ersjournals.com)
  • Rommens and coworkers found that the CF gene is located in a region on the long arm of chromosome 7, locus 31. (contemporaryobgyn.net)
  • By incorporating microfluidics and sequential labelling strategies can be employed to "walk" along chromosomes to visualize chromosome regions that form distinct structures in the form of compartments. (bruker.com)
  • Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. (thefreedictionary.com)
  • Page 169 - Liu, YG and Whittier, RF (1995) Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from PI and YAC clones for chromosome walking. (google.com)
  • Mapping involves (1) dividing the chromosomes into smaller fragments that can be propagated and characterized and (2) ordering (mapping) them to correspond to their respective locations on the chromosomes. (lsu.edu)
  • Cosmid vectors for rapid genomic walking, restriction mapping, and gene transfer. (atcc.org)
  • That way, the short part of the long DNA that is sequenced keeps "walking" along the sequence. (infomutt.com)
  • A method of determining the DNA sequence of a chromosome by extending out from a known region, designing a new sequencing primer to read out from the newly sequenced region and continuing until the desired section of DNA has been sequenced. (sigmaaldrich.com)
  • Chromosome jumping enables two ends of a DNA sequence to be cloned without the middle section. (wikipedia.org)
  • Large chromatin loops containing several megabases of DNA were observed extending outwards from the surface of the domain defined by the specific chromosome 6 paint. (biologists.org)
  • Data regarding the prognostic relevance of cytogenetic abnormalities in older patients with AML receiving intensive chemotherapy are limited, and only 2 studies have assessed the impact of specific chromosome aberrations in elderly patients treated according to a single protocol. (bloodjournal.org)
  • The lab captures molecular data to reveal information about the chromosome's contours and topological behaviour at the instant of fixation for investigation--noting of course that chromosome are fluid and dynamic, flexing and changing as they constantly transition between different states (work/ gene expression/protein manufacture and cell division). (thefreelibrary.com)
  • Chromosome jumping is a tool of molecular biology that is used in the physical mapping of genomes. (wikipedia.org)
  • This makes it possible to 'walk' along the chromosome from one gene to the next, to reach a desired LOCUS . (thefreedictionary.com)
  • The nucleotide composition of this locus is significantly different from that of the rest of this pathogenicity island, suggesting a different ancestry and a mosaic structure for this region of the S. typhimurium chromosome. (asm.org)
  • Karyotype structure and chromosome fragility in the grass Phleum echinatum Host. (ipk-gatersleben.de)
  • Date presented, up to 8 megabases of human chromosome 19, will show that in addition to regional compartments, there are suggestions that there may be differential organization of chromosomes in maternal and paternal homologues. (bruker.com)
  • The large-scale chromatin organization of the major histocompatibility complex and other regions of chromosome 6 was studied by three-dimensional image analysis in human cell types with major differences in transcriptional activity. (biologists.org)
  • Other work has looked at developing a method using Vectorette PCR in order to accomplish genomic walking. (wikipedia.org)
  • Despite its very different genomic environment from Hoxb, Hoxd also loops out from its chromosome territory, and unfolds, upon activation in differentiating embryonic stem (ES) cells and in the tailbud of the embryo. (biologists.org)
  • Useful for constructing genomic libraries and chromosome walking and for gene transfer using dihydrofolate reductase as a selectable marker. (atcc.org)
  • Individual genomic regions showed distinct configurations in relation to the chromosome 6 terrritory. (biologists.org)
  • This webinar shows how OligoSTORM and single molecule localization microscopy can be used to visualize in situ chromosome structure. (bruker.com)
  • Chromosome microdissection, FISH, scanning electron microscopy and confocal laser scanning microscopy. (ipk-gatersleben.de)
  • During interphase, a mammalian chromosome exists as a highly organized supramolecular globule in the nucleus. (portlandpress.com)
  • 1994 ). Visualization of G1 chromosomes: A folded, twisted, supercoiled chromonema model of interphase chromatid structure. (biologists.org)
  • Understanding chromosome structure in situ is critical in understanding normal as well as pathological functioning of the nucleus. (bruker.com)
  • In prokaryotes, or cells without a nucleus, the chromosome is merely a circle of DNA. (encyclopedia.com)
  • In eukaryotes, or cells with a distinct nucleus, chromosomes are much more complex in structure. (encyclopedia.com)
  • There are 46 chromosomes inside the nucleus and you can number them from #1 to #46. (hubpages.com)
  • To assess the prognostic impact of cytogenetics in elderly patients with acute myeloid leukemia (AML) receiving intensive induction and consolidation treatment according to a single protocol specifically designed for patients above age 60, pretreatment samples from 361 patients registered for the AML HD98-B trial of the German-Austrian AML Study Group were analyzed by chromosome banding and fluorescence in situ hybridization, and cytogenetic findings were correlated with outcome. (bloodjournal.org)
  • Hello, I am interested in obtaining chromosome walks from any region on the X, preferably in a cosmid vector, the larger the better. (bio.net)
  • A walk from the Tamkun cosmid library would be ideal. (bio.net)
  • We show that, in the limb bud of the embryonic day 9.5 embryo, where Hoxd is also activated, there is visible decondensation of chromatin but no detectable movement of the region out from the chromosome territory. (biologists.org)
  • Cytogenetic mapping with centromeric BAC contigs shows that this recombination-poor region comprises more than half of barley chromosome 3H. (ipk-gatersleben.de)
  • We conclude that evolutionarily conserved chromosome remodelling mechanisms, predating the duplication of mammalian Hox loci, underlie Hox regulation along the rostrocaudal embryonic axis. (biologists.org)
  • Today we know that a chromosome contains a single molecule of DNA along with several kinds of proteins. (encyclopedia.com)
  • A single molecule of DNA within a chromosome may be as long as 8.5 centimeters (3.3 inches). (encyclopedia.com)
  • These data suggest markers Xfcg17 and Xfcg9 will provide excellent starting points for chromosome walking and the eventual map-based cloning of Tsn1. (thefreedictionary.com)
  • Other alleles of mus101 causing different phenotypes were later isolated: a female sterile allele results in a defect in a tissue-specific form of DNA synthesis (chorion gene amplification) and lethal alleles cause mitotic chromosome instability that can be observed genetically and cytologically. (genetics.org)
  • In 1998, we constructed a dense linkage map mainly based on RAPD markers consisting of 28 LGs corresponding to the 28 chromosomes of B. mori ( Y asukochi 1998 ). (genetics.org)
  • To further study the role of chromosome 3p allele loss in the pathogenesis of lung cancer, we performed high resolution loss of heterozygosity (LOH) studies on 97 lung cancer and 54 preneoplastic/preinvasive microdissected respiratory epithelial samples using a panel of 28 3p markers. (aacrjournals.org)
  • A genetic linkage map shows the relative locations of specific DNA markers along the chromosome. (lsu.edu)
  • Two markers located near each other on the same chromosome will tend to be passed together from parent to child. (lsu.edu)
  • with this procedure large regions of the chromosome can be spanned. (thefreedictionary.com)
  • Allele loss involving chromosome arm 3p is one of the most frequent and earliest known genetic events in lung cancer pathogenesis and may affect several potential tumor suppressor gene regions. (aacrjournals.org)
  • to describe two large, unstable DNA regions of the chromosome of uropathogenic Escherichia coli ( 23 ). (asm.org)
  • Chromosome jumping is used to bypass regions difficult to clone, such as those containing repetitive DNA, that cannot be easily mapped by chromosome walking, and is useful in moving along a chromosome rapidly in search of a particular gene. (wikipedia.org)
  • More recently, it has become apparent that virulence factors are frequently found in discrete contiguous regions of the chromosome termed pathogenicity islands ( 33 , 43 , 54 ). (asm.org)
  • We individually validate 153 probes and take advantage of our probe repository to quantify the extent of intermingling between multiple heterologous chromosome pairs, showing a much higher extent of intermingling in human embryonic stem cells compared to fibroblasts. (nature.com)
  • Dr. Boeke and his team made more than 500 alterations to its genetic base, removing repeating sections of some 47,841 DNA base pairs, deemed unnecessary to chromosome reproduction and growth. (newswise.com)
  • To fit within a chromosome, the DNA molecule has to be twisted and folded into a very complex shape. (encyclopedia.com)
  • A circular DNA molecule that is replicated and segregated by the same mechanisms as the chromosome. (sigmaaldrich.com)
  • A chromosome is a single long DNA molecule assembled along its length with nucleosomes and proteins. (portlandpress.com)
  • Brenda Gee, of the Office of the Executive Vice Chancellor and Provost, and Jennifer Dowd, of Campus Life Services, added some extra color to the foggy San Francisco morning at AIDS Walk 2016. (ucsf.edu)
  • At Boston Dynamics out of Waltham, MA, engineers are building a robot that can walk naturally and simulate the motion of a real human. (medgadget.com)
  • After fertilization, a fetus (with 46 chromosomes) develops that carries one mutated APC. (hubpages.com)
  • This procedure has the potential to replace the difficult procedure of microcloning, as well as facilitate chromosome walking. (nih.gov)
  • Unit 5.4 Construction of Chromosome Jumping and Linking Libraries in E.coli. (wikipedia.org)
  • The 102-kb region of Y. pestis is an evolutionarily stable linkage of an HPI with a pigmentation segment in a region of the chromosome prone to rearrangement in vitro. (asm.org)
  • 17 ). This region is deleted en bloc at a frequency of 10 −5 , probably by homologous recombination between its two flanking IS 100 copies ( 39 ). (asm.org)
  • The ETR1 gene was cloned by the method of chromosome walking. (sciencemag.org)
  • A fast air-dry dropping chromosome preparation method suitable for FISH in plants. (ipk-gatersleben.de)
  • H. floribundum and H. iseranum individuals of the Krkono e exhibit a single, unusually large "marker" chromosome, which occurs only very rarely in H. piloselliflorum and which is completely lacking in H. pilosella . (cas.cz)
  • The mapping strategy is shown to be successful in simulations with portions of the X chromosome already largely mapped into overlapping YACs by other means. (wustl.edu)
  • A chromosome is a structure that occurs within cells and that contains the cell's genetic material. (encyclopedia.com)
  • During ES cell differentiation, decondensed alleles can also be found inside of chromosome territories, and loci that have looped out of the territories can appear to still be condensed. (biologists.org)
  • and chromosome territories (CTs) has been correlated with active transcription. (biologists.org)
  • Most small cell lung carcinomas (91%) and squamous cell carcinomas (95%) demonstrated larger 3p segments of allele loss, whereas most (71%) of the adenocarcinomas and preneoplastic/preinvasive lesions had smaller chromosome areas of 3p allele loss. (aacrjournals.org)
  • Be sure to label the different segments of the chromosomes. (columbia.edu)
  • In silico protein modeling followed by interaction analysis between Pi54 protein model and selected four candidate effector proteins models revealed that Mo-01947_9 protein model encoded by a gene located at chromosome 4 of M. oryzae , interacted best at the Leucine Rich Repeat domain of Pi54 protein model. (frontiersin.org)
  • 70 percent) caused by a mutation in a gene located on chromosome 7, whose protein product is the cystic fibrosis transmembrane conductance regulator (CFTR). (newworldencyclopedia.org)
  • b) Illustrate how these chromosomes would pair during meiosis. (columbia.edu)
  • The way DNA strands fold control which bits of a chromosome are active, in communication with each other and neighbouring strands, and which bits are not. (thefreelibrary.com)
  • The characteristic architecture of a chromosome is due to the way DNA is wound around histones. (chromosomewalk.ch)