Chromosome Painting: A technique for visualizing CHROMOSOME ABERRATIONS using fluorescently labeled DNA probes which are hybridized to chromosomal DNA. Multiple fluorochromes may be attached to the probes. Upon hybridization, this produces a multicolored, or painted, effect with a unique color at each site of hybridization. This technique may also be used to identify cross-species homology by labeling probes from one species for hybridization with chromosomes from another species.PaintingsProcyonidae: A family of long-tailed terrestrial omnivores including RACCOONS, ringtails, and coatimundis.Chromosome Banding: Staining of bands, or chromosome segments, allowing the precise identification of individual chromosomes or parts of chromosomes. Applications include the determination of chromosome rearrangements in malformation syndromes and cancer, the chemistry of chromosome segments, chromosome changes during evolution, and, in conjunction with cell hybridization studies, chromosome mapping.Karyotype: The full set of CHROMOSOMES presented as a systematized array of METAPHASE chromosomes from a photomicrograph of a single CELL NUCLEUS arranged in pairs in descending order of size and according to the position of the CENTROMERE. (From Stedman, 25th ed)Trichechus manatus: Member of the genus Trichechus inhabiting the coast and coastal rivers of the southeastern United States as well as the West Indies and the adjacent mainland from Vera Cruz, Mexico to northern South America. (From Scott, Concise Encyclopedia Biology, 1996)Chromosomes: In a prokaryotic cell or in the nucleus of a eukaryotic cell, a structure consisting of or containing DNA which carries the genetic information essential to the cell. (From Singleton & Sainsbury, Dictionary of Microbiology and Molecular Biology, 2d ed)Canidae: A family of terrestrial carnivores with long snouts and non-retractable claws. Members include COYOTES; DOGS; FOXES; JACKALS; RACCOON DOGS; and WOLVES.In Situ Hybridization, Fluorescence: A type of IN SITU HYBRIDIZATION in which target sequences are stained with fluorescent dye so their location and size can be determined using fluorescence microscopy. This staining is sufficiently distinct that the hybridization signal can be seen both in metaphase spreads and in interphase nuclei.Chromosomes, Mammalian: Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of MAMMALS.Karyotyping: Mapping of the KARYOTYPE of a cell.Chromosome Mapping: Any method used for determining the location of and relative distances between genes on a chromosome.Chromosome Aberrations: Abnormal number or structure of chromosomes. Chromosome aberrations may result in CHROMOSOME DISORDERS.Translocation, Genetic: A type of chromosome aberration characterized by CHROMOSOME BREAKAGE and transfer of the broken-off portion to another location, often to a different chromosome.Chromosomes, Human: Very long DNA molecules and associated proteins, HISTONES, and non-histone chromosomal proteins (CHROMOSOMAL PROTEINS, NON-HISTONE). Normally 46 chromosomes, including two sex chromosomes are found in the nucleus of human cells. They carry the hereditary information of the individual.Characiformes: An order of fresh water fish with 18 families and over 1600 species. The order includes CHARACINS, hatchetfish, piranhas, and TETRAS.Pitheciidae: A family of New World monkeys in the infraorder PLATYRRHINI consisting of two subfamilies: Callicebinae and Pitheciinae.X Chromosome: The female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in human and other male-heterogametic species.Felidae: The cat family in the order CARNIVORA comprised of muscular, deep-chested terrestrial carnivores with a highly predatory lifestyle.Cytogenetics: A subdiscipline of genetics which deals with the cytological and molecular analysis of the CHROMOSOMES, and location of the GENES on chromosomes, and the movements of chromosomes during the CELL CYCLE.Viverridae: The family of civets which are small and medium-sized Old World carnivores, often striped or spotted.Carnivora: An order of MAMMALS, usually flesh eaters with appropriate dentition. Suborders include the terrestrial carnivores Fissipedia, and the aquatic carnivores PINNIPEDIA.Metaphase: The phase of cell nucleus division following PROMETAPHASE, in which the CHROMOSOMES line up across the equatorial plane of the SPINDLE APPARATUS prior to separation.DNA Probes: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.Medicine in ArtPaintSex Chromosomes: The homologous chromosomes that are dissimilar in the heterogametic sex. There are the X CHROMOSOME, the Y CHROMOSOME, and the W, Z chromosomes (in animals in which the female is the heterogametic sex (the silkworm moth Bombyx mori, for example)). In such cases the W chromosome is the female-determining and the male is ZZ. (From King & Stansfield, A Dictionary of Genetics, 4th ed)Chromosomes, Human, Pair 1: A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.Evolution, Molecular: The process of cumulative change at the level of DNA; RNA; and PROTEINS, over successive generations.Phylogeny: The relationships of groups of organisms as reflected by their genetic makeup.Chromosomes, Human, Pair 7: A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.ArtChromosomes, Bacterial: Structures within the nucleus of bacterial cells consisting of or containing DNA, which carry genetic information essential to the cell.Chromosome Segregation: The orderly segregation of CHROMOSOMES during MEIOSIS or MITOSIS.Chromosomes, Human, Pair 11: A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.Chromosomes, Human, Pair 17: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.Chromosome Deletion: Actual loss of portion of a chromosome.Chromosomes, Plant: Complex nucleoprotein structures which contain the genomic DNA and are part of the CELL NUCLEUS of PLANTS.Chromosomes, Human, Pair 6: A specific pair GROUP C CHROMSOMES of the human chromosome classification.Chromosomes, Human, Pair 9: A specific pair of GROUP C CHROMSOMES of the human chromosome classification.Chromosomes, Human, Pair 21: A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.Chromosomes, Human, Pair 13: A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.Species Specificity: The restriction of a characteristic behavior, anatomical structure or physical system, such as immune response; metabolic response, or gene or gene variant to the members of one species. It refers to that property which differentiates one species from another but it is also used for phylogenetic levels higher or lower than the species.Chromosomes, Fungal: Structures within the nucleus of fungal cells consisting of or containing DNA, which carry genetic information essential to the cell.Genome: The genetic complement of an organism, including all of its GENES, as represented in its DNA, or in some cases, its RNA.Chromosomes, Human, 6-12 and X: The medium-sized, submetacentric human chromosomes, called group C in the human chromosome classification. This group consists of chromosome pairs 6, 7, 8, 9, 10, 11, and 12 and the X chromosome.Chromosomes, Human, Pair 12: A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.Chromosome Disorders: Clinical conditions caused by an abnormal chromosome constitution in which there is extra or missing chromosome material (either a whole chromosome or a chromosome segment). (from Thompson et al., Genetics in Medicine, 5th ed, p429)Chromosomes, Human, Pair 2: A specific pair of human chromosomes in group A (CHROMOSOMES, HUMAN, 1-3) of the human chromosome classification.Chromosomes, Human, Pair 16: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.Chromosomes, Human, Pair 22: A specific pair of GROUP G CHROMOSOMES of the human chromosome classification.Chromosome Pairing: The alignment of CHROMOSOMES at homologous sequences.Chromosomes, Artificial, Bacterial: DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintenance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.Chromosomes, Human, Pair 5: One of the two pairs of human chromosomes in the group B class (CHROMOSOMES, HUMAN, 4-5).Chromosomes, Human, Pair 4: A specific pair of GROUP B CHROMOSOMES of the human chromosome classification.Lymphocytes: White blood cells formed in the body's lymphoid tissue. The nucleus is round or ovoid with coarse, irregularly clumped chromatin while the cytoplasm is typically pale blue with azurophilic (if any) granules. Most lymphocytes can be classified as either T or B (with subpopulations of each), or NATURAL KILLER CELLS.Chromosomes, Human, Pair 10: A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.Chromosomes, Human, Y: The human male sex chromosome, being the differential sex chromosome carried by half the male gametes and none of the female gametes in humans.Chromosomes, Human, Pair 8: A specific pair of GROUP C CHROMOSOMES of the human chromosome classification.Chromosomes, Human, Pair 19: A specific pair of GROUP F CHROMOSOMES of the human chromosome classification.Chromosome Breakage: A type of chromosomal aberration involving DNA BREAKS. Chromosome breakage can result in CHROMOSOMAL TRANSLOCATION; CHROMOSOME INVERSION; or SEQUENCE DELETION.Chromosomes, Human, X: The human female sex chromosome, being the differential sex chromosome carried by half the male gametes and all female gametes in humans.Chromosomes, Human, 1-3: The large, metacentric human chromosomes, called group A in the human chromosome classification. This group consists of chromosome pairs 1, 2, and 3.Chromosomes, Human, Pair 15: A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.HumanitiesChromosomes, Human, Pair 14: A specific pair of GROUP D CHROMOSOMES of the human chromosome classification.Chromosomes, Human, Pair 18: A specific pair of GROUP E CHROMOSOMES of the human chromosome classification.Chromosomes, Human, 16-18: The short, submetacentric human chromosomes, called group E in the human chromosome classification. This group consists of chromosome pairs 16, 17, and 18.Chromosomes, Human, Pair 20: A specific pair of GROUP F CHROMOSOMES of the human chromosome classification.Chromosomes, Artificial, Yeast: Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.Chromosome Inversion: An aberration in which a chromosomal segment is deleted and reinserted in the same place but turned 180 degrees from its original orientation, so that the gene sequence for the segment is reversed with respect to that of the rest of the chromosome.Chromosomes, Human, 13-15: The medium-sized, acrocentric human chromosomes, called group D in the human chromosome classification. This group consists of chromosome pairs 13, 14, and 15.

Suppression of tumorigenicity in human ovarian cancer cell lines is controlled by a 2 cM fragment in chromosomal region 6q24-q25. (1/191)

Multiple distinct regions of chromosome 6 are frequently affected by losses of heterozygosity in primary human ovarian carcinomas. We introduced a normal human chromosome 6 into HEY and SKOV-3 ovarian carcinoma cell lines using microcell-mediated chromosome transfer techniques to further investigate the role of this chromosome in ovarian tumorigenesis. The exogenous chromosome was stably propagated in the recipient cells based on fluorescence in situ hybridization (FISH) analyses with a chromosome 6 painting probe. The tumorigenicity of HEY and SKOV-3 cells was completely suppressed after transfer of chromosome 6, but not after transfer of a chromosome 11q13-qter fragment used as control. Using 46 polymorphic microsatellite markers, the region bounded by D6S1649 and D6S1564 was found to be commonly deleted in HEY: chromosome 6 tumorigenic revertant clones. The boundaries of the commonly deleted region could be further narrowed down to a 2 cM (based on the Whitehead genetic map) or 0.36 megabase (based on gdb mapping data) region between D6S1637 and D6S1564 after transferring the exogenous chromosome from revertants into mouse L cells and performing allelic deletion mapping studies against this mouse background. We conclude that this region contains a tumor suppressor gene important for the control of ovarian tumor development.  (+info)

Comparison of AluI-induced frequencies of dicentrics and translocations in human lymphocytes by chromosome painting. (2/191)

It has been shown repeatedly that following irradiation of human lymphocytes in the G0 stage, more translocations are induced than dicentrics. To check the role of DNA double-strand breaks (DSB) alone for the induction of symmetrical and asymmetrical chromosome aberrations, the frequencies of induced exchange aberrations by the restriction enzyme AluI were analyzed. The enzyme was introduced into cells using the pellet pipetting technique. Frequencies of induced translocations and dicentrics were determined using a chromosome painting assay with chromosome-specific DNA libraries for chromosomes 1, 4 and X (representing 16.8% of the human genome). The number of translocations detected was approximately 3-fold higher than the number of dicentrics, indicating that the increased frequency of translocations compared with dicentrics found in irradiated human lymphocytes does not result from DNA lesions other than DSB but from differential processing of DSB.  (+info)

Analysis of bleomycin-induced chromosomal aberrations in Chinese hamster primary embryonic cells by FISH using arm-specific painting probes. (3/191)

Chinese hamster primary embryonic cells (at G1 phase) were treated with 1.0 or 3.0 microg/ml bleomycin and chromosomal aberrations in first division metaphases were analysed by fluorescence in situ hybridization (FISH) using arm-specific painting probes for chromosomes 3, 4, 8 and 9. We observed that bleomycin induced all classes of chromosome-type aberrations very efficiently. The interesting findings were: (i) the frequency of induced interstitial translocations (i.e. insertions) was approximately equal to that of reciprocal translocations; (ii) the frequency of induced pericentric inversions was higher than that of centric rings. In our earlier studies, we found that X-rays induced a low frequency of interstitial translocations in comparison with reciprocal translocations and equal frequencies of centric rings and pericentric inversions. These data suggest that bleomycin differs from X-rays with respect to the induction of some specific types of aberrations. The results of a chi2 test examining the hypothesis that formed aberrations among the chromosomes or chromosome arms are randomly distributed on the basis of their relative lengths revealed a differential involvement of these chromosomes in the aberrations following exposure to bleomycin. In general, chromosome 8 was found to be more involved in induced aberrations than expected, chromosome 4 was randomly involved, whereas chromosomes 3 and 9 were less involved. This study demonstrates the utility of arm-specific painting probes for efficient detection of a large variety of chromosomal aberrations induced by bleomycin.  (+info)

The application of comparative genomic hybridization and fluorescence in situ hybridization to the characterization of genotoxicity screening tester strains AHH-1 and MCL-5. (4/191)

AHH-1 TK+/- is a human B cell-derived lymphoblastoid cell line that constitutively expresses a high level of the cytochrome CYP1A1. The MCL-5 cell line was developed by transfection of AHH-1 with cDNAs encoding the human cytochrome P450s, CYP1A2, CYP2A6, CYP2E1, CYP3A4 and microsomal epoxide hydrolase carried in plasmids. The metabolic components of these cell lines make them a useful screening tool for use in mutagenicity studies. Although AHH-1 and MCL-5 are closely related, the two cell lines show differences which cannot be attributed to transfection. In the present study both cell lines were investigated for chromosome stability by comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) using whole chromosome probes and telomeric probes. Amplification in chromosomes 4q, 3q and 9p was observed in both cell lines. To compare the cell lines directly, AHH-1 and MCL-5 DNAs were co-hybridized on the same metaphases using a modified CGH technique. The only difference observed between AHH-1 and MCL-5 was the degree of amplification involving the subtelomeric region of chromosome 4; the additional telomeric region (4q) was translocated onto chromosome 11 and/or chromosome X. FISH was use to show the presence of isochromosomes 3q and 9p in both cell lines with a chromosome number of 48 or higher. These data demonstrate that CGH and FISH with chromosome-specific probes are able to resolve complex karyotypes and to highlight subchromosomal regions involved in rearrangements and potential chromosome fragile sites. Analyses such as those described here may be of considerable value in the determination of the stability of a variety of the cell lines used in the mutagenicity testing of chemicals.  (+info)

Multiplex-FISH for pre- and postnatal diagnostic applications. (5/191)

For >3 decades, Giemsa banding of metaphase chromosomes has been the standard karyotypic analysis for pre- and postnatal diagnostic applications. However, marker chromosomes or structural abnormalities are often encountered that cannot be deciphered by G-banding alone. Here we describe the use of multiplex-FISH (M-FISH), which allows the visualization of the 22 human autosomes and the 2 sex chromosomes, in 24 different colors. By M-FISH, the euchromatin in marker chromosomes could be readily identified. In cases of structural abnormalities, M-FISH identified translocations and insertions or demonstrated that the rearranged chromosome did not contain DNA material from another chromosome. In these cases, deleted or duplicated regions were discerned either by chromosome-specific multicolor bar codes or by comparative genomic hybridization. In addition, M-FISH was able to identify cryptic abnormalities in patients with a normal G-karyotype. In summary, M-FISH is a reliable tool for diagnostic applications, and results can be obtained in +info)

Association of chromosome territories with the nuclear matrix. Disruption of human chromosome territories correlates with the release of a subset of nuclear matrix proteins. (6/191)

To study the possible role of the nuclear matrix in chromosome territory organization, normal human fibroblast cells are treated in situ via classic isolation procedures for nuclear matrix in the absence of nuclease (e.g., DNase I) digestion, followed by chromosome painting. We report for the first time that chromosome territories are maintained intact on the nuclear matrix. In contrast, complete extraction of the internal nuclear matrix components with RNase treatment followed by 2 M NaCl results in the disruption of higher order chromosome territory architecture. Correlative with territorial disruption is the formation of a faint DNA halo surrounding the nuclear lamina and a dispersive effect on the characteristically discrete DNA replication sites in the nuclear interior. Identical results were obtained using eight different human chromosome paints. Based on these findings, we developed a fractionation strategy to release the bulk of nuclear matrix proteins under conditions where the chromosome territories are maintained intact. A second treatment results in disruption of the chromosome territories in conjunction with the release of a small subset of acidic proteins. These proteins are distinct from the major nuclear matrix proteins and may be involved in mediating chromosome territory organization.  (+info)

Spatial relationship between transcription sites and chromosome territories. (7/191)

We have investigated the spatial relationship between transcription sites and chromosome territories in the interphase nucleus of human female fibroblasts. Immunolabeling of nascent RNA was combined with visualization of chromosome territories by fluorescent in situ hybridization (FISH). Transcription sites were found scattered throughout the territory of one of the two X chromosomes, most likely the active X chromosome, and that of both territories of chromosome 19. The other X chromosome territory, probably the inactive X chromosome, was devoid of transcription sites. A distinct substructure was observed in interphase chromosome territories. Intensely labeled subchromosomal domains are surrounded by less strongly labeled areas. The intensely labeled domains had a diameter in the range of 300-450 nm and were sometimes interconnected, forming thread-like structures. Similar large scale chromatin structures were observed in HeLa cells expressing green fluorescent protein (GFP)-tagged histone H2B. Strikingly, nascent RNA was almost exclusively found in the interchromatin areas in chromosome territories and in between strongly GFP-labeled chromatin domains. These observations support a model in which transcriptionally active chromatin in chromosome territories is markedly compartmentalized. Active loci are located predominantly at or near the surface of compact chromatin domains, depositing newly synthesized RNA directly into the interchromatin space.  (+info)

Gain of chromosomes 15 and 19 is frequent in both mouse hepatocellular carcinoma cell lines and primary tumors, but loss of chromosomes 4 and 12 is detected only in the cell lines. (8/191)

Chromosomal alterations were investigated in hepatocellular carcinoma cell lines, primary tumors and liver epithelial cell lines derived from normal livers of C57BL/6JxC3H/HeJ F(1) and C3H/HeJxC57BL/6J F(1) mice. In the primary tumors, non-random gain of chromosomes 15 and 19 was found in seven and five of 14 hepatocellular carcinomas, respectively. On the other hand, in the cases of both liver epithelial and hepatocellular carcinoma cell lines, frequent changes were loss of chromosomes 4 (4/9 cell lines) and 12 (3/9) as well as gain of chromosomes 15 (5/9) and 19 (4/9). These results indicate that the chromosomal gain is associated with both in vivo carcinogenesis and establishment of cell lines, while the loss is specific for the latter. PCR analysis using polymorphic microsatellite DNA markers revealed that the loss of chromosome 12 as well as chromosome 4 was much more frequent for the C57BL/6J hepatocarcinogenesis-resistant rather than the susceptible C3H/HeJ strain.  (+info)

Comparative chromosome painting of pronghorn (Antilocapra americana) and saola (Pseudoryx nghetinhensis) karyotypes with human and dromedary camel ...
Parrots ( order: Psittaciformes) are the most common captive birds and have attracted human fascination since ancient times because of their remarkable intelligence and ability to imitate human speech. However, their genome organization, evolution and genomic relation with other birds are poorly understood. Chromosome painting with DNA probes derived from the flow-sorted macrochromosomes (1-10) of chicken (Gallus gallus, GGA) has been used to identify and distinguish the homoeologous chromosomal segments in three species of parrots, i. e., Agapornis roseicollis (peach-faced lovebird); Nymphicus hollandicus (cockatiel) and Melopsittacus undulatus ( budgerigar). The ten GGA macrochromosome paints unequivocally recognize 14 to 16 hybridizing regions delineating the conserved chromosomal segments for the respective chicken macrochromosomes in these representative parrot species. The cross-species chromosome painting results show that, unlike in many other avian karyotypes with high homology to ...
In addition to that quick fix, i decided to test the accuracy of SupportMixs chromosome paintings by juxtaposing them over the MDLP-World22 chromosome graphs. Due to time limitations, i used only first 7 chromosomes of my own SNP data. At first, i ran the MDLP-World22 modification of DIYDodecad v2.1 in byseg mode on "windows" of 500 contiguous SNPs along a chromosome, slided by increments of 50. After that i cut out chromosome paintings of each chromosome from SupportMixs graphic output and aligned them to the scale of corresponding DIYDodecad chromosome graphs: ...
sundoc Migration; Titel: Chromosome painting and arrangement of interphase chromosome territories in Arabidopsis thaliana, Verfasser: Pecinka, Ales, 2005 ; Halle, Saale : Universitäts- und Landesbibliothek
The Chromosome Painting feature is an in-depth way of looking at your Ancestry Composition results. It shows a colorful representation of...
Fluorescence in situ hybridization (FISH) plays an essential role in research and clinical diagnostics. The versatility and resolution of FISH depends critically on the probe set used. Here, we descri
XMP are chromosome-specific and comprise mouse whole chromosome painting probes which are directly labeled with an emitting fluorochrome.
CHO cells have become the most important cell system for the biotechnological production of pharmaceuticals. Reasons for that are described in the article The increasing potential of chinese hamster ovary cells. A critical feature of a cell line used for production of therapeutics is to be clonal, which means origination from a single cell, to ensure a homogenous therapeutic product. The demonstration of clonal derivation can be a big effort for the pharmaceutical industry, but does it really ensure a homogenous product quality?. ...
Rice yield is a highly complex trait and is controlled by a large number of QTLs with small individual effects. CSSLs are appropriate for detecting QTLs with both large and small effects, and reciprocal CSSLs confer the advantage of enabling evaluation of differences in allelic effects of QTLs in both genetic backgrounds. If a detected QTL shows the same gene activity in reciprocal genetic backgrounds, that locus should have no genetic interaction or epistasis with other background factor(s). QTLs that show different gene activity in the reciprocal backgrounds may be involved in genetic interaction or epistasis with other background factor(s) [21]. By using reciprocal CSSLs derived from a cross between Takanari and Koshihikari, we detected a number of QTLs underlying brown rice yield and its components. Among these loci, we confirmed that the Takanari alleles of GN1a and APO1 increased the number of spikelets per panicle in the reciprocal backgrounds (Figures 5 and 6). A QTL for the number of ...
Chromo(A) Essay, Research Paper Chromosome Probes at the University of Toronto Sensitive chromosome probes recently discovered by aUniversity of Toronto geneticist will make it easierto detect certain types of genetic and prenataldiseases, as well as being used to determine paternityand provide forensic evidence in criminal cases.
Many mesenchymal neoplasms are often associated with some nonrandom cytogenetic abnormalities (36) . In the case of ALT, several studies have observed the presence of supernumerary ring and/or long marker chromosomes in 93% of these neoplasms (3 , 37) . Interestingly, chromosome painting by fluorescence in situ hybridization revealed that these marker chromosomes mostly contain DNA sequences from the long arm of chromosome 12 (3) . In particular, the 12q13-15 region is found to be amplified within these aberrant chromosomes. This region contains several genes (such as MDM2, CDK4, and HMGA2) that play a direct role in human tumorigenesis (4 , 38 , 39) . These cytogenetic findings are consistent with previous data that describe the HMGA2 gene as a frequent target of rearrangements because of chromosomal translocations that involve the 12q13-15 region (11, 12, 13, 14) .. In this study, we analyzed HMGA2 gene expression in 12 primary cell cultures derived from ALTs. RT-PCR showed 58% of these tumors ...
Looking for online definition of chromosome painting in the Medical Dictionary? chromosome painting explanation free. What is chromosome painting? Meaning of chromosome painting medical term. What does chromosome painting mean?
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This study presents a whole-genome comparison of human and a representative of the Afrotherian clade, the African elephant, generated by reciprocal Zoo-FISH. An analysis of Afrotheria genomes is of special interest, because recent DNA sequence comparisons identify them as the oldest placental mammalian clade. Complete sets of whole-chromosome specific painting probes for the African elephant and human were constructed by degenerate oligonucleotide-primed PCR amplification of flow-sorted chromosomes. Comparative genome maps are presented based on their hybridization patterns. These maps show that the elephant has a moderately rearranged chromosome complement when compared to humans. The human paint probes identified 53 evolutionary conserved segments on the 27 autosomal elephant chromosomes and the X chromosome. Reciprocal experiments with elephant probes delineated 68 conserved segments in the human genome. The comparison with a recent aardvark and elephant Zoo-FISH study delineates new ...
From works by Botticelli and Raphael to Salvador Dali and Frida Kahlo, discover the paintings that have shaken the art world through the centuries and across continents. Great Paintings takes you on your own personal gallery tour of over 60 of the worlds best-loved paintings. Unlock the hidden meanings and symbols behind each painting, with over 700 photographs to bring the pictures to life and help you understand the key features, composition and techniques that have made these paintings stand out. Plus, biographies of the artists provide the background to each art work, inspiring you to paint your own picture of the historical and social context behind each masterpiece. Updated for 2018, Great Paintings is a beautiful guide to the paintings, both familiar and new, that have changed the world- it really is like having a gallery of all the great paintings at your fingertips.. Previous ISBN: 9781405363303. ...
The Klimt University of Vienna Ceiling Paintings, also known as the Faculty Paintings, were a series of paintings made by Gustav Klimt for the ceiling of the University of Viennas Great Hall between the years of 1900-1907. In 1894, Klimt was commissioned to paint the ceiling. Upon presenting his paintings, Philosophy, Medicine and Jurisprudence, Klimt came under attack for pornography and perverted excess in the paintings. None of the paintings would go on display in the university. In May 1945 all three paintings were destroyed by retreating SS forces. Philosophy was the first of the three pictures presented to the Austrian Government at the seventh Vienna Secession exhibition in March 1900. It had been awarded a gold medal at the World Exhibition in Paris, but was attacked by those in his own country. Klimt described the painting as follows: "On the left a group of figures, the beginning of life, fruition, decay. On the right, the globe as mystery. Emerging below, a figure of light: ...
Oil painting, landscape, portrait, photo, abstract, flower, animal, animals. oil painting technique oil painting reproduction original oil painting discount oil painting oil painting landscape oil painting world oil painting for sale portrait oil painting artist oil painting abstract oil painting art of oil painting still life oil painting Nude oil painting oil on canvas painting clean oil painting china oil painting chinese oil painting ...
Nijmegen breakage syndrome is an autosomal recessive disorder characterized by microcephaly, immunodeficiency, hypersensitivity to X-irradiation, and a high predisposition to cancer. Nibrin, the product of the NBN gene, is part of the MRE11/RAD50 (MRN) complex that is involved in the repair of DNA double strand breaks (DSBs), and plays a critical role in the processing of DSBs in immune gene rearrangements, telomere maintenance, and meiotic recombination. NBS skin fibroblasts grow slowly in culture and enter early into senescence. Here we present an incidental finding. Skin fibroblasts, derived from a 9 year old NBS patient, showed a mosaic of normal diploid cells (46,XY) and those with a complex, unbalanced translocation. The aberrant karyotype was analysed by G-banding, comparative genomic hybridization, and whole chromosome painting. The exact breakpoints of the derivative chromosome were mapped by whole genome sequencing: 45,XY,der(6)(6pter → 6q11.1::13q11 → 13q21.33::20q11.22 → 20qter),-13.
The company has had interaction with universities. It has received the Yarrowia lipolytica expressing system from the University of Boroda for marketing purposes.. The company also works with the Environmental Protection Training & Protection Institute. It is presently working with the Center for Cellular & Molecular Biology (CCMB) to provide rapid, accurate, and reliable diagnostic service based on modern molecular methods to cancer patients. CCMB and Biological Evans also continue to work together on the preparation and use of human chromosome painting probes, training for the chromosomal and molecular cytogenetic diagnosis of human genetic disorders, DNA diagnosis of human genetic disorders, isolation, maintenance and production of stem cells, and the building of gene chips.. Sources: ...
Why care about painting as an art? Does it offer to engage our aesthetic interest in ways that other art forms do not, or does it merely reproduce the aesthetic satisfactions they provide? Most paintings involve both marks on a surface, and something represented by those marks. Some attempts to say what is distinctive about painting concentrate on the former feature, understanding the art as an exploration of the two-dimensional picture plane. Others concentrate on the representational aspect, seeking to find something special about the things painting can represent, or the way in which it achieves this. The most promising approaches acknowledge both aspects, and do so as essential elements in the experiencewe have of painting. One such approach turns on the idea that the configurational aspect inflects the representational, so that what we see in the picture itself somehow involves the marks from which the painting is composed. Another sees painting as offering aesthetic values found ...
The first burst of energy can be frantic when performing in front of the camera because I am trying to paint over a large area in a short time. The second stage is where specific areas do not measure up to favorite areas. Quick fixes invite picking at the painting. To avoid picking, I kept saying I wanted to keep the whole painting in mind and work all over but I could not avoid this stage. The next step is obvious - a rest where the painting is put away or just left in view where the mind can subconsciously work on it. The next stage is returning to the canvas after working on others so the canvas is no longer precious and I can try painting all over the canvas to resolve perhaps a new goal. The last step should be the signing of the painting in which a few details are carefully selected for revision. Revisions are to be consistent and important to the meaning of the painting. Sometimes after the signing, I continue to want to make changes ...
Mandana paintings News: Latest and Breaking News on Mandana paintings. Explore Mandana paintings profile at Times of India for photos, videos and latest news of Mandana paintings. Also find news, photos and videos on Mandana paintings - Page 12
The fascinating art of Name Painting is a recent introduction to the United States. In New York City, where many Name Painters now work, the practice of this art dates back less than 10 years. But in China, where this activity originated, its history is thousands of years old. Although it developed from Chinese calligraphy, Name Painting has completely evolved and now stands alone as a form of art. The colors, the images, the constantly changing nature of Name Painting, and its emphasis on unique expression make it unlike any other kind of artistic creation. If you have ever seen someone name painting, you will always remember the experience. Both children and adults are mesmerized watching the quick, fluid movements of Name Painters painting a person s name with colorful figures and objects, using water-based paints. People of all ages are thrilled to receive a banner with the letters of their name hand painted in a style of this art form. Now everyone can learn how to Name Paint with this book, How to
I have continued to write a series of articles on painting for Answers.com as their Category Expert Writer for that subject.. Most of them are roughly split between "how-to" articles on painting tools and techniques, and history-oriented articles on individual artists, art movements and particular paintings.. You can see my most recent contributions at the top of the Painting.Answers.com page. I maintain a more complete linked list of my Answers.com articles here on Lines and Colors / Answers.. Here are my topics for November and December of 2013:. December 2013. Visual Aids for Painting ...
Shawn Deitch is a contemporary impressionist painter who splits her time between Carmel, Indiana and Tucson, Arizona. She draws inspiration from nature and has a special interest in Indiana wetlands & lakes and Sonoran washes and vistas. Many of her paintings are plein air painted on site in the open air. Shawn has studied oil painting with regional and national artists including Jerry Points, Pamela Newell, David Slonim, Wyatt LeGrand, Debra Huse, Albert Handell, and Shelby Keefe. ----------------------------- Art expands my world - and a very colorful world at that! Rich, vivid colors frequently fill my work saturating the painting with emotion. The great majority of my paintings are alla prima, even the large pieces. I love the satisfaction of beginning and finishing a painting in one day, even if its a very, very long day. Art brings me joy and hopefully will bring joy to you as well ...
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Buy original abstract oil paintings, contemporary acrylic art, unique art work, mystical, surrealism and realism art online, acryl paintings, acrylic paintings for sale, acrylic paintings on canvas, acrylic paintings for sale by Zunuzin
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Buy online or call 1-800-929-2431. An Archival New Panel Made for the Unique Demands of Encaustic Painting. Only Encausticbord has a ready to use surface formulated for the unique demands of encaustic painting and mixed media. Encaustic gesso is applied to Ampersands sealed Hardbord panel to form a bright, smooth and velvety surface that is ready to use with the ancient technique of encaustic painting. The ground is not only heat resistant and highly absorbent, but also holds tight to layers of wax and collage without the...
Cambio (UK) sell mouse paints which are labelled with Cy 3, biotin, or FITC. Alternativly you could make your own if you can get hold of some monochromosomal mouse hybrids and pcr the mouse chromosome ...
I work with rat tumors and try to establish their karyotype. Is there anyone who knows wher I can get such paints?? Bernd K lsch Cell-biology 45147 Essen, Germany e-mail: ttu3a0 at aixrs1.hrz.uni-essen.de knowq s where I can ...
Video articles in JoVE about cytogenetic analysis include Expedited Radiation Biodosimetry by Automated Dicentric Chromosome Identification (ADCI) and Dose Estimation, 2D and 3D Chromosome Painting in Malaria Mosquitoes, Analysis of the Ambient Particulate Matter-induced Chromosomal Aberrations Using an In Vitro System, Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice, Chromosome Preparation From Cultured Cells, An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis, Heterogeneity Mapping of Protein Expression in Tumors using Quantitative Immunofluorescence, Spectral Karyotyping to Study Chromosome Abnormalities in Humans and Mice with Polycystic Kidney Disease, Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids, High-throughput
Get a home painting estimate by calling a painter or painting company or by filling out an estimate form on painting company websites. Franklin Painting, CertaPro Painters, Personal Painters, Five...
This course is not to about a new style of painting, rather it is a process-based experience to create a medicine painting for our lives. As we travel around the Medicine Wheel and process an intent or situation through Painting we create layers intuitively. Our culminating painting contains several lessons, insights and understandings we have moved through in the 4 directions and stages of life through the Wheel. ...
This painting (oil and acrylic on canvas, 48"x36") by Lewis J. Thomas, Jr., M.D., former Director of the Biomedical Computer Laboratory and Associate Director of Institute for Biomedical Computing at Washington University, commemorates the 50th anniversary of the founding of the computer labs. Click the image above to enlarge. The following description of the BCL-CSL Commemorative Painting was provided by the artist.. This painting is about the interactive laboratory computing that began in 1964 at the Washington University Medical School (WUMS). The paintings background depicts the console and early keyboard of the LINC (Laboratory INstrument Computer). The LINC stimulated the founding of two computer labs at WUMS, both in the old Shriners Hospital Building: the Biomedical Computer Lab (BCL) and Computer Research Lab (CRL), later renamed the Computer Systems Lab (CSL).. As Gordon Bell put it, "LINC was the worlds first personal computer." As such, it changed the way computers came to be used ...
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Detail záznamu - An Improved Consensus Linkage Map of Barley Based on Flow-Sorted Chromosomes and Single Nucleotide Polymorphism Markers - Detail záznamu - Knihovna Akademie věd České republiky
Italian artist Agostino Arrivabene uses antique painting techniques to create a foundation from which metamorphic figures emerge in moments of creation. The time-consuming labor of grinding pigments and layering paints is evident in the complex, heavily textural works. New worlds hide beneath and within cracks and crinkles as human-like figures manifest above ground and often out of water.. The Milan-based artist is strongly influenced by 16th century Italian Mannerism, an anti-classical style in which body forms were elongated and light was intrinsically important. In one painting, a man with lifeless pale-blue skin looms like a giant over green moss. Oceanic vegetation rises from his shoulders and illuminated critters surround his head like a halo, as if to suggest a Jesus figure rising from the depths of the ocean. This is not the only image focused on the human head, as several paintings by Arrivabene creating moments of struggle and heaviness around the cranium of his figures - perhaps as a ...
♦ Title: Untitled Media: Professional grade artist acrylic paint. ♦Support: 14 inches by 10 inches Original Acrylic painting on canvas panel Date: 2012 ♦Additional information: Bright yellow daisies, accompanied by fresh oranges. Listing is for painting only, and comes unframed. ♦About
This report will relate about the main results of the analyses carried out on the rock paintings of several Cappadocia churches. The analyses have been carried out within the survey "Rock paintings in Cappadocia. For a project of knowledge, conservation and valorization of the church of the Forty Martyrs at Şahinefendi and its territory" directed by Prof. Maria Andaloro of Tuscia University (Italy) . Such analyses have been carried out according to a methodological path tested during the several years of surveys in Turkey, particularly on the island of Küçük Tavşan and on many other sites in the territory of Mandalya Gulf and also on the occasion of other research projects . This research mission is part of a bigger project called "For a data bank of wall paintings and mosaics of Asia Minor (4th-15th centuries: images, materials, techniques of execution (1996-2010)" directed by Prof. Maria Andaloro. The aim of this work concerns the understanding of the constitutive materials, the execution ...
Starting from the problems of describing non-Western visual aesthetic systems ethnographically, this article provides a basic account of yonata, painting with design, among the Piro people of the Bajo Urubamba river in Eastern Peru. Yonata, and its product yonchi, `design, is the Piro variant of the Amazonian practice of painting surfaces with designs of `geometric type. Placing this practice in the context of the Piro lived world, I argue that painting with design encodes the ideal life trajectory of a Piro woman. For Piro people, design is beautiful, and through design women demonstrate the success of their own lives as beauty on the bodies of others and certain material objects. In the conclusion, I extend the analysis presented here to Australian Aboriginal and Melanesian cases, and to the Western tradition.. ...
In terms of the painting process, mostly are on the color mixing. Massive masking needed for the armors (the plastic for this kit is a bit too soft so i decided not to separate those part that have connected). As i mention, I painted ZZ gundam before which mean painting this kit without armor can be a lot faster than painting the armors. Leg unit is the most tricky part for painting which make my compressor overheat during the painting process. I usually separate the painting process into few session which chest, waist, arms and head in one session, legs in one session, armor piece in one session and backpack with weapon one session. All armor piece were painted in one session and my compressor overheat for two times during the painting process. The only thing i feel from the painting process was it need lot of time and patient as there are lot of large size parts ...
Possessing a multi-faceted set of interests, Davis began painting in 1997, and attributes much of his inspiration to watching his mother pursue craft projects during his childhood, and immersing himself in art history throughout his career," the gallery says. "From helping his mother with activities ranging from woodworking, hand-tooling leather, and remodeling the familys home, to engaging with ancient art and Netherlandish painting from the Northern Renaissance, Davis is a self-taught painter, who continues to render complex paintings of surrealist life, embedded with symbols of mortality, folly, and hubris, fixated within whimsical compositions.". See more on 111 Minnas site and the artists page.. ...
A new process for producing a reproduction of a painting or the like wherein the reproduction has a relief surface which imitates the relief surface of the painting or the like. Heat and pressure are applied simultaneously to an assembly consisting of a mold of the painting, a colored sheet for coloring the reproduction and a substrate to retain the reproduction. Subsequent to this step, shock cooling is applied. The reproduction thus produced does not suffer from the rebound effect of the prior art and good color integrity is maintained.
Modern landscape painting by the artist Osnat Tzadok. Choose from thousands of modern, contemporary and abstract paintings in this online art gallery. Artwork: Field of Happiness, dimensions: 40x24. #8021
Join Ethan as he shows you how to eliminate the struggle of creating "mud" in oil painting. In his demo he will cover breaking the oil painting process down into 3 steps representing three different days of work on one painting ...
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Our Online Painting Contest awards over $23,650 every month. Enter online right now via your web browser. The BoldBrush Painting Contest - sponsored by FASO Artist Websites
Our Online Painting Contest awards over $23,650 every month. Enter online right now via your web browser. The BoldBrush Painting Contest - sponsored by FASO Artist Websites
See painting silver reflex or other paintings from the collection modern. More than 4000 paintings and photos to decorate your home.
Marthas dad, who is in vaudeville, unexpectedly shows up at home. Her father tells her that hes been very successful with his act but that he cannot stay and has to catch the next train to Syracuse. The next day Martha begins her week as an upstairs maid, cleaning the first floor. When she goes in to clean the gallery, she discovers four paintings hanging on the walls, neatly in a row, each draped over with a sheet. Martha finds the paintings intriguing but doesnt know much about them. The first painting, titled Proserpine by Rossetti looks to Martha like Eve holding the apple. However, Alphonse informs her the painting is not about Eve and the fruit is definitely NOT an apple. Before they can talk further, the two are discovered by Marthas Ma who sends Alphonse off and tells Martha that the paintings are Miss Roses pride and her ticket into New York society. Ma explains that although the majority of the paintings are in her rooms, "Miss Rose gets it in her head that certain paintings need ...
Summary A high-dimensional metabolomics screen to diagnose and monitor chronic myeloid leukemia (CML) has been developed. Over 3000 metabolites are detected to yield a ‘cytogenetic signature’ that confirms the presence of cells bearing the Philadelphia chromosome, a genetic marker for CML. Through comparison of the levels of these metabolites to those found in the blood of disease-free individuals, diagnosis can be made as to the presence and severity of the cancer using only one blood sample. This rapid and sensitive analytic technology reduces time and overall cost associated with CML diagnosis and enhances patient comfort and well-being.
We employed fluorescence in situ hybridization (FISH) with probes established by flow sorting metaphase chromosomes of the domestic cat (Felis catus, 2n = 38) to paint homologous segments on human chromosomes and, reciprocally, using human chromosome paints on feline metaphase preparations. The results revealed, by direct microscopic observation, widespread conservation of genome organization between the two mammalian orders and confirmed 90% of the homologous genes mapped to both species. Fourteen of 23 human chomosomes were hybridized with single cat probes, and 9 of 19 cat chromosomes were entirely labeled by a single human probe. All other chromosomes were labeled with only two or, at most, three probes of the respective species. Y-chromosome probes gave no signals. Approximately 30 syntenic segments were identified, and the number of translocations could be estimated to be on the order of one new translocation per 10 million years in the phylogenetic lines leading to human and cat. Using the
00290] Karyotype analysis. Karyotype analysis was performed by Multicolor FISH analysis (M-FISH). Subconfluent STAPS cells were arrested in metaphase by colcemid (final concentration 0.270 μg/ml) to the culture medium for 2.5 h at 37°C in 5% C02. Cells were washed with PBS, treated with trypsin/ethylenediaminetetraacetic acid (EDTA), resuspended into cell medium and centrifuged for 5 min at 1200 rpm. To the cell pellet in 3 ml of PBS, 7 ml of a prewarmed hypotonic 0.0375 M KC 1 solution was added. Cells were incubated for 20 min at 37°C. Cells were centrifuged for 5 min at 1200 rpm and the pellet was resuspended in 3-5 ml of 0.0375 M KC1 solution. The cells were fixed with methanol/acetic acid (3: 1; vol/vol) by gently pipetting. Fixation was performed four times prior to spreading the cells on glass slides. For the FISH procedure, mouse chromosome-specific painting probes were combinatorially labeled using seven different fluorochromes and hybridized as previously described (Jentsch et al, ...
00290] Karyotype analysis. Karyotype analysis was performed by Multicolor FISH analysis (M-FISH). Subconfluent STAPS cells were arrested in metaphase by colcemid (final concentration 0.270 μg/ml) to the culture medium for 2.5 h at 37°C in 5% C02. Cells were washed with PBS, treated with trypsin/ethylenediaminetetraacetic acid (EDTA), resuspended into cell medium and centrifuged for 5 min at 1200 rpm. To the cell pellet in 3 ml of PBS, 7 ml of a prewarmed hypotonic 0.0375 M KC 1 solution was added. Cells were incubated for 20 min at 37°C. Cells were centrifuged for 5 min at 1200 rpm and the pellet was resuspended in 3-5 ml of 0.0375 M KC1 solution. The cells were fixed with methanol/acetic acid (3: 1; vol/vol) by gently pipetting. Fixation was performed four times prior to spreading the cells on glass slides. For the FISH procedure, mouse chromosome-specific painting probes were combinatorially labeled using seven different fluorochromes and hybridized as previously described (Jentsch et al, ...
Looking for some new parts or paint for your vehicle? Check out Horton Brothers Painting, the highest quality auto body shop in Bishop. Youll be amazed at the results this place can get out of your car when it repairs any unattended scratches and dents.So stop in at Horton Brothers Painting today for all your vehicles body work needs.
I spent the entire day on the porch painting, using the color charts I made the day before. (See previous post) The first few of the eight paintings were closer to the real color of the landscape. As I became more comfortable with the acrylics, I relied more on the value range in front of…
Here is a painting in progress. The pastel paintings are done very much in the same way an oil painting is done. I start with a base color. Usually I work with a precolored surface. With oil painting I would lay in a foundation color so the white canvas wouldnt show through. So this is the same basis. Since I am right handed I work from left to right unless it is a landscape or still life in which case the entire surface is worked uniformly. With pet portraits, so I dont damage an area that is completed, the surface is worked from left to right. While working an area of the subject I lay in mid tone values first - then work light to dark. With pastels an artist can "fill the tooth" of the surface so it is important not to over work any areas. Once the entire subject is painted I go back and reinforce the lightest lights and darkest darks. My greatest area of interest is the eyes. If the eyes arent right the entire painting will look odd. If the painting is about a sleeping animal it is ...
...Traditional Swedish bonad paintings can contain toxic substances such ...Bonad paintings are painted wall hangings that tell a story often wit... Its important to know about the materials used in these wall-hanging...Several toxic pigments have been found in the wall hangings studied i...,Colorful,wall,hangings,contain,toxic,substances,biological,biology news articles,biology news today,latest biology news,current biology news,biology newsletters
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Find out how to correctly prepare walls, timber and brickwork for painting including how to mask up and prime surfaces and block strains to get a great finish
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Commissioning a painter to color from a photo is a nice awesome type of art which you can use to change a little photo right into a bigger and much more impressive artwork. An attractive painting, a family member hung on your wall is much more accessible or visible to buddies and relatives than the usual photo hidden within the pages of the family album. A colored portrait also allows you to maintain a picture for memorial purposes. For the best painting from photo visit us today ...
I am marveling at the club in the top painting of Bam-Bam. It is so tastefully modeled -- the tones give a real sense of realism combined with cartoony shapes and squiggles -- a perfect balance that doesnt compete with the flatter parts of the painting. The club is so convincing, but with such a strange but fun color for a piece of wood -- giving it a quality that borders on plastic and metallic (I guess its the highlight) -- its both real and unreal which is what makes it amazing to me. That is the best Bam-Bam art Ive ever seen. ...
Gordeckaya LIST. Russian popular artistic answer; there is with middle. 19 digits In district Propulsion Gorodets, Nizhny Novgorod Oblast. Flamboyant, laconic section painting of (genre the scene, shapes Trojan, roosters, flower patterns), by free smear with a white and black graphical obvodkoy, decorates distaffs have, furniture, the shutters, doors. In 1936 is based artel (with 1960 factory of Gordeckaya painting of ), izgotovlyayuschaya souvenirs; masters of D. I. Kryukov, A. E. Konovalov, I. A. Mazin.. Gordeckaya LIST on tree, traditional artistic answer, rebuffed development in mid-19 century in villages on River Uzole in neighborhood Gorodets, Nizhny Novgorod Oblast Nizhny Novgorod area.. Occurrence Luster Takes the beginning of in production gorodetskikh pryalochnykh Donets, encrusted finish morenym oak and decorated highly carving. Unlike widely common of distaffs, vytesannykh from single wooden monolith, gorodetskie distaffs have were staffed from two parts: Donets River and crest. ...
Artist. Contemporary Painting. Any paintings posted here are for sale unless otherwise stated. The sketches posted here were from my Moleskine journal until August 1, 2017 (when I switched to sketching on a wider variety of unbound papers) and the originals are not for sale. However, I can sometimes sell reproductions of the sketches. (Exceptions: Sometimes my sketches are practice work based directly on art by other artists. I will not sell reproductions based on any of these ...
A Man Takes A Risks. is a painting by Corinne de la garrigue which was uploaded on January 2nd, 2010. The painting may be purchased as wall art, home decor, apparel, phone cases, greeting cards, and more. All products are produced on-demand and shipped worldwide within 2 - 3 business days.
Handmade oil painting reproduction of The Table Still Life With Almonds 1907 by Maurice de Vlaminck - on canvas and available in any size or choose other oil painting reproductions from artisoo.com.
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In the years that I spent painting my story of abuse, I was looking through the few family photos that I had in my possession. One of the things that struck me, was the fact that whilst all this abuse, pain and trauma occurred on a daily basis. There we were, portrayed as a typical,happy family, caught on camera-for all to see. I wanted to correct that warped perception. To expose the truth and reality of the lies and deceit. Of what truly lies beneath these happy family images. So, I rendered a typical family portrait. And- charged with subconscious emotions, I applied my true feelings without any conscious thought. Through my brush and onto a blank, empty space. Thus creating this image, which is a manifestation of my emotional trauma. The main theme of this painting is of innocence and trust destroyed, by a family members psychopathy and sexual gratification. The uncles smile depicts his malevolence, his eyes do not smile, only the mouth. Which contains exaggerated clenched teeth and thinly ...
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Yellow Hibiscus is a painting by Arline Wagner which was uploaded on December 20th, 2009. The painting may be purchased as wall art, home decor, apparel, phone cases, greeting cards, and more. All products are produced on-demand and shipped worldwide within 2 - 3 business days.
For the moment...renaming this forum Movies, Music AND Art. A Banksy painting of Girl with Balloon, started to SHRED itself just seconds after a buyer paid $1.4 million at auction. The painting is now said to be worth twice as much. Performance Art! https://www.indy100.com/article/banksy-sothebys-auction-art-prank-video-8572991
Big load ahead! Here is my digital painting method for figures as requested by Rebecca. This is just one painting method of many out there but it works nicely for me. I use low opacity combined with a textured brush in …
Painting Services in in Golden Pages. The finish of every construction activity is painting. It is usually done in several layers and is a guarantee for the long-term use of the building
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A continuation of painting concepts explored in AR201, designed to further acquaint students with technical processes, formal relationships, and conceptual issues. Structured assignments employing direct observation (including the figure) and invention provide a context for development of a personal vision. Other assignments will refer to historical and contemporary movements and painting methods with readings and discussions.
Handmade oil painting reproduction of Torso 1929 by Kazimir Malevich - on canvas and available in any size or choose other oil painting reproductions from artisoo.com.
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The Blue Boy painting was purchased in 1921 for $725,000. The Blue Boy has not been on auction since. Adjusting for inflation and assuming the painting has not appreciated in value, $725,000...
The techniques for painting floors are the same as for painting any other large flat surface. Learn more in this home improvement article.
Heres a painting from last night ✨ Always fun to have a painting buddy by your side too @bessliart - #candacemckay candkay (Candace McKay) shared for
Neurohormonal activation in heart failure is well established and involves activation of the renin-angiotensin-aldosterone network and the sympathetic nervous system. Associated with this process is the enhanced production of cytokines and other inflammatory factors that have been directly implicated in the pathophysiological progression of heart failure (2, 6, 11, 15). These factors have both direct and indirect effects on cardiac remodeling and can also affect gene expression in other tissues (19). Due to the systemic nature of the disease, we tested the hypothesis that noncardiac tissue can exhibit specific gene expression signatures that associate with cardiovascular events.. By performing blood gene expression profiling analysis, we analyzed ,27,000 transcripts in blood and demonstrated for the first time that blood gene expression varies as a function of patient outcomes (see online data supplement).1 A total of 197 mortality genes were discovered. Intriguingly, a large number ...
The spatial arrangement of some genetic elements relative to chromosome territories and in parallel with the cell nucleus was investigated in human lymphocytes. The structure of the chromosome territories was studied in chromosomes containing regions ( clusters) of highly expressed genes (HSA 9, 17) and those without such clusters ( HSA 8, 13). In chromosomes containing highly expressed regions, the elements pertaining to these regions were found close to the centre of the nucleus on the inner sides of chromosome territories; those pertaining to regions with low expression were localized close to the nuclear membrane on the opposite sides of the territories. In chromosomes with generally low expression ( HSA 8, 13), the elements investigated were found symmetrically distributed over the territories. Based on the investigations of the chromosome structure, the following conclusions are suggested: (1) Chromosome territories have a non-random internal 3D structure with defined average mutual ...
The information in the present section on francis-bacon.com is based on the data in Francis Bacon: Catalogue Raisonné by Martin Harrison and Rebecca Daniels, which was published by The Estate of Francis Bacon in 2016. The following Notes for readers are extracted from the catalogue raisonné (Vol.1, p.102 and 103) and elaborate on the methodology and thinking behind the compilation and presentation of some data, such as titles, dates and media.. Notes for readers. Paintings are catalogued chronologically, under the year of their completion: thus a painting dated 1956-57 will be found in 1957. Undocumented paintings, to which only approximate (circa) dates can be attached, are generally placed at the end of the year in which they are believed to have been painted; this rule is departed from when there is firm evidence that a painting was made at a specific date during a certain year (for example Street Scene (with Car in Distance), 1984 (84-03).. Titles of paintings placed in inverted ...
The information in the present section on francis-bacon.com is based on the data in Francis Bacon: Catalogue Raisonné by Martin Harrison and Rebecca Daniels, which was published by The Estate of Francis Bacon in 2016. The following Notes for readers are extracted from the catalogue raisonné (Vol.1, p.102 and 103) and elaborate on the methodology and thinking behind the compilation and presentation of some data, such as titles, dates and media.. Notes for readers. Paintings are catalogued chronologically, under the year of their completion: thus a painting dated 1956-57 will be found in 1957. Undocumented paintings, to which only approximate (circa) dates can be attached, are generally placed at the end of the year in which they are believed to have been painted; this rule is departed from when there is firm evidence that a painting was made at a specific date during a certain year (for example Street Scene (with Car in Distance), 1984 (84-03).. Titles of paintings placed in inverted ...
Watercolours are a great form of indoor painting. Theyre much less mess than poster paint and easy to wipe up if youre using a plastic mat or lino. They also handily come with a whole palette of colours! These are great for encouraging creative messy play, colours (and colour mixing), fine motor control, and learning…
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Some discussions Ive been involved with lately have got me thinking about the relationship between color and value. Do you approach them as two different steps of a painting? For example some people see a well worked out monochromatic grisaille painting as perhaps the most important step in a painting, with the scumbled on color as an accentuating element. On the other hand some people have a more Alla Prima approach where they jump right into color, and create the illusion of "turning" surfaces and value directly through color mixing. How long do you spend on the monochromatic stage of a painting ...
The relative location of 2 repetitive DNAs, i.e. ribosomal (rDNA) and a tandemly repeated satellite DNA (satDNA), with respect to the centromere, suggested that B chromosomes in the grasshopper |i&
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EPAs 2008 Lead-Based Paint Renovation, Repair and Painting (RRP) Rule (as amended in 2010 and 2011), aims to protect the public from lead-based paint hazards associated with renovation, repair and painting activities. These activities can create hazardous lead dust when surfaces with lead paint, even from many decades ago, are disturbed. The rule requires workers to be certified and trained in the use of lead-safe work practices, and requires renovation, repair, and painting firms to be EPA-certified. These requirements became fully effective April 22, 2010.. Read EPAs regulations on residential property renovation at 40 CFR 745, Subpart E.. ...
Three subspecies of banteng (Bos javanicus) have been described: B. j. javanicus in Java, B. j. lowi in Borneo, and B. j. birmanicus in Cambodia, Lao PDR, Myanmar, Thailand and Vietnam. In this paper we provide the first description of the karyotype of the Cambodian banteng. The chromosomal complement of B. j. birmanicus differs from that of B. j. javanicus, which was previously found to be similar to that of cattle, Bos taurus (2n = 60). The Cambodian banteng karyotype has a diploid number of 2n = 56 (FN = 62) and the karyotype consists of 26 pairs of acrocentric chromosomes and two pairs of submetacentric chromosomes. Comparisons with other species of the subtribe Bovina show that the two pairs of bi-armed chromosomes resulted from two centric fusions involving the equivalent of cattle chromosomes 1 and 29, and 2 and 28, respectively. Cross-species fluorescence in-situ hybridization (FISH) with B. taurus whole chromosome paints and satellite DNA I probes was used to identify the chromosomes ...
In this exhibition, many French people and oil painting reproductions artists by the soul of China painting hui, hiding in succession. Have people told reporters: "lets happy about see art from China, the exhibition of Chinese paintings is a higher artistic level, embodies the Chinese present art of the defunct dynasty and natural instinct, longed to see more in the future in the bliss." Painting the old young artists in the Chinese entrants kingson brought his work at Paris. When it comes to the the exhibition of painting works, he shows: "the event new lesson is called" entropy ", is the latest in the series, expect ShiBian expand interdisciplinary art dimensions from philosophical Angle, from the Angle of art, considering the relationship between human and technology." When it comes to the current sea internalization of Chinese custom oil painting art, kingson: "for years to come, the inevitable with more and more overseas Chinese art market recognition, and the contrast salon chairman ...
THURSDAY, NOVEMBER 20, 2014 at 4:30pm at the Environmental Science Center (21 Sachem Street, 1st Floor, Lecture Hall 110). Abstract: The UNESCO world heritage site, Mogao caves near Dunhuang, is a Buddhist temple site along the ancient Silk Road with a history that extends over 1000 years from the 4th C to the 14th C. The 45,000 square metres of wall paintings in 492 caves are an immense resource for the study of the history of art, architecture, religion, technology, politics and cultural exchange along the Silk Road. In this talk, we show an example of a systematic in situ study of the wall paintings using a range of non-invasive optical imaging and spectroscopic instruments. Microfade spectrometry, PRISMS (the remote 3D spectral imaging system), hand-held X-ray Fluorescence spectrometry (XRF), and Optical Coherence Tomography (OCT) will all be discussed. This talk demonstrates through examples how a combination of non-invasive imaging and spectroscopic methods can yield a wealth of ...
In the case of Dedvukaj v. Maloney, the U.S. District Court for the Eastern District of Michigan has held that it can exercise personal jurisdiction over a New York eBay seller accused of breach of contract, fraud and misrepresentation in connection with an auction that went sour.. Plaintiff Maloney, a Michigan resident, won two eBay auctions for paintings from defendant Dedvukaj, a seller based in Syracuse, New York. The auctions advertised the paintings as being originals, and the defendant apparently verified the authenticity of the paintings over the phone. After the auctions closed, the plaintiff sought to collect the art works, but the defendant never shipped them. (There appears to be a dispute over whether the defendant was selling original paintings or copies.) The plaintiff refused a refund and demanded either the original paintings or their fair market value.. The plaintiff filed a lawsuit in his home state of Michigan alleging breach of contract, fraud and misrepresentation. The ...
Chromosome analysis and sorting (library construction, chromosome paint). *DNA copy number variation (by Flow-FISH or BACs-on- ... aka Paint-A-Gate),[15] VenturiOne, CellQuest Pro, Infinicyt or Cytospec.[16] Once the data is collected, there is no need to ...
Chromosome painting is currently the main experimental technique. Recently, researchers have developed computational methods to ... Watterson, G.A.; Ewens, W.J.; Hall, T.E.; Morgan, A. (1982). "The chromosome inversion problem". Journal of Theoretical Biology ... Sturtevant, A. H.; Dobzhansky, T. (1936). "Inversions in the Third Chromosome of Wild Races of Drosophila Pseudoobscura, and ... Murphy, W. J. (2005). "Dynamics of Mammalian Chromosome Evolution Inferred from Multispecies Comparative Maps". Science. 309 ( ...
"Chromosome painting in the manatee supports Afrotheria and Paenungulata". BMC Evolutionary Biology. 7 (6): 6. doi:10.1186/1471- ...
Fauth E, Zankl H (1999). "Comparison of spontaneous and idoxuridine-induced micronuclei by chromosome painting". Mutat Res. 440 ...
"Phylogenomic study of spiral-horned antelope by cross-species chromosome painting". Chromosome Research: an International ... It was found that chromosomes involved in centric fusions in these species used a complete set of cattle painting probes ... The eland have 31 male chromosomes and 32 female chromosomes. In a 2008 phylogenomic study of spiral-horned antelopes, ... The study confirmed the presence of the chromosome translocation known as Robertsonian translocation (1;29), a widespread ...
"Phylogenomic study of spiral-horned antelope by cross-species chromosome painting". Chromosome Research. 16 (7): 935-47. doi: ... The nyala has 55 male chromosomes and 56 female chromosomes. The Y chromosome has been translocated onto the 14th chromosome, ... as in other tragelaphids, but no inversion of the Y chromosome occurs. Cranial studies have shown that the mountain nyala and ...
"Congruent distribution of Neolithic painted pottery and ceramic figurines with Y-chromosome lineages". Antiquity. 76 (293): 707 ... J-P58 is the most common Y-Chromosome haplogroup among men from all of this region. J-M267 is uncommon in most of Northern and ... Nebel, A; Filon, D; Brinkmann, B; Majumder, P; Faerman, M; Oppenheim, A (2001). "The Y chromosome pool of Jews as part of the ... Nov 2004). "Y chromosome haplogroups of elite Ethiopian endurance runners". Hum. Genet. 115 (6): 492-7. doi:10.1007/s00439-004- ...
... whole chromosomes of one species to metaphase chromosomes of another species. Comparative chromosome painting allows a rapid ... "Cytogenetic Analysis by Chromosome Painting Using Dop-Pcr Amplified Flow-Sorted Chromosomes". Gene Chromosome Canc. 4 (3): 257- ... microdissection of chromosomes and chromosome regions was also used to obtain probes for chromosome painting. Best results were ... Total human chromosomes or their arms can efficiently paint extended chromosome regions in many placentals down to Afrotheria ...
King R, Underhill PA (2002). "Congruent distribution of Neolithic painted pottery and ceramic figurines with Y-chromosome ... The Y-Chromosome Consortium treeEdit. This is the official scientific tree produced by the Y-Chromosome Consortium (YCC). The ... In human genetics, Haplogroup J-M172 or J2[Phylogenetics 1] is a Y-chromosome haplogroup which is a subclade (branch) of ... Family Tree DNA uses the Y-Chromosome Consortium tree and posts it on their website. ...
"Cross-species chromosome painting in the golden mole and elephant-shrew: support for the mammalian clades Afrotheria and ...
Listed as Endangered (B1ab(ii,iii,v)+2ab(ii,iii,v) Robinson, T.; Yang, F. & Harrison, W. (2002). "Chromosome painting refines ...
Robinson, T. J.; Yang, F.; Harrison, W. R. (2002). "Chromosome painting refines the history of genome evolution in hares and ... The Pronolagus chromosomes have undergone four fusions and one fission from the Lagomorpha ancestral state (2n=48), which ... All species in this genus have 21 pairs of chromosomes (2n = 42). The karotype for P. rupestris has been published. ... doi:10.1080/02541858.1982.11447806 . Robinson, T. J. (1980). "Comparative chromosome studies in the family Leporidae ( ...
... using chromosome painting and G-banding". BMC Evolutionary Biology. 8: 169. doi:10.1186/1471-2148-8-169. PMC 2435554 . PMID ...
... using chromosome painting and G-banding". BMC Evol. Biol. 8: 169. doi:10.1186/1471-2148-8-169. PMC 2435554 . PMID 18534011. ...
... using chromosome painting and G-banding". BMC Evolutionary Biology. 8: 169. doi:10.1186/1471-2148-8-169. PMC 2435554 . PMID ... The wedge-capped capuchin has a diploid chromosome number of 52, though some others in the genus have 54 chromosomes. Nine ... human chromosomes correspond to those of the C. olivaceus. Phylogenetic analysis and constructions of cladograms have ...
Robinson, T., Yang, F., & Harrison, W. (2002). "Chromosome painting refines the history of genome evolution in hares and ... "Rediscovered Hula painted frog 'is a living fossil'". BBC News. Retrieved 4 June 2013. Scientists find lamprey a 'living fossil ... Hula painted frog (Latonia nigriventer) Purple frog (Nasikabatrachus sahyadrensis) Jawless fish Hagfish (Myxinidae) Family Bony ...
Meller V; Wu K; Roman G.; Kuroda M; Davis R (1997). "roX1 RNA paints the X chromosome of male Drosophila and is regulated by ... As males only contain one X chromosome, male flies dosage compensate for the X chromosome by hyper-transcribing the X ... It was also shown that in the presence of mutant roX RNA the MSL complex was unable to localize on the X chromosome and ... This is achieved by the MSL complex binding to the X chromosome and inducing histone H4 lysine 16 acetylation and allows for ...
Chromosome Painting: Principles, Strategies and Scope, Chromosome Techniques: Theory and Practice, Chromosome Techniques - A ... ISBN 978-1-57808-413-5. Arun Kumar Sharma; Archana Sharma (27 June 2011). Chromosome Painting: Principles, Strategies and Scope ... Arun Kumar Sharma; Archana Sharma (27 June 2011). Chromosome Painting: Principles, Strategies and Scope. Springer Science & ... Considered by many as the father of Indian cytology, he headed the Centre for Advanced Study on Cell and Chromosome at the ...
Toder (Jun 1997). "Comparative chromosome painting between two marsupials: origins of an XX/XY1Y2 sex chromosome system.". ... "Comparative chromosome painting of chicken autosomal paints 1-9 in nine different bird species". Cytogenetics and Genome ... "Chromosome painting shows that skunks (Mephitidae, Carnivora) have highly rearranged karyotypes". Chromosome Res. 16 (8): 1215- ... "Chromosome painting refines the history of genome evolution in hares and rabbits (order Lagomorpha)". Cytogenics and Genetic ...
"Comparative chromosome painting defines the karyotypic relationships among the domestic dog, Chinese raccoon dog and Japanese ... Comparative chromosome painting defines the karyotypic relationships among the domestic dog, Chinese raccoon dog and Japanese ...
"Painting a specific chromosome with CRISPR/Cas9 for live-cell imaging," Cell Res doi:10.1038/cr.2017.9 (2017). Chong, Shasha; ... "Live births after simultaneous avoidance of monogenic diseases and chromosome abnormality by next-generation sequencing with ... Chromosome structure and dynamics, and Genomic instability; Technological: Single-molecule imaging, Single-cell genomics, ...
Red Panda and Five Mustelid Species Revealed by Comparative Chromosome Painting and G-banding". Chromosome Research. 10 (3): ... The domesticated cat and its closest wild ancestor are both diploid organisms that possess 38 chromosomes and roughly 20,000 ... owing to the clear depictions of house cats in Egyptian paintings about 3,600 years old. However, in 2004, a Neolithic grave ...
Cross-species chromosome painting in bats from Madagascar: the contribution of Myzopodidae to revealing ancestral syntenies in ... non-sex chromosomes). The X chromosome is submetacentric (with one arm slightly longer than the other) and the Y chromosome is ... The animal has a karyotype of 46 chromosomes, with a total of 50 major arms on the autosomes ( ... the number of chromosomes and arms is identical in M. griveaudi, the Malagasy M. aelleni and M. gleni, and even the Asian M. ...
... red panda and five mustelid species revealed by comparative chromosome painting and G-banding". Chromosome Res. 10 (3): 209-22 ... The domesticated cat and its closest wild ancestor are both diploid organisms that possess 38 chromosomes and roughly 20,000 ...
This is often called "whole-chromosome painting." If every possible probe is used, every chromosome, (the whole genome) would ... Probes that hybridize along an entire chromosome are used to count the number of a certain chromosome, show translocations, or ... Special locus-specific probe mixtures are often used to count chromosomes, by binding to the centromeric regions of chromosomes ... A technique known as chromosome combing is increasingly used for this purpose. The extended conformation of the chromosomes ...
2012). "Genomic restructuring in the Tasmanian devil facial tumour: Chromosome painting and gene mapping provide clues to ... chromosomes. More recently evolved strains have an additional mutant marker chromosome, for a total of fourteen chromosomes. ... Tasmanian devil cells have 14 chromosomes; the oldest-known strain of the tumour cells have thirteen chromosomes, nine of which ... Among the mutations present in the tumour genome is trisomy in chromosome 5p, as well as several single base mutations, and ...
In recent investigations, it has also been made clear that both varieties have the same chromosome number (n=15) and can be ... A 19th-century Japanese painting depicting Shennong: Chinese legends credit Shennong with the invention of tea.[26] ...
"Congruent distribution of Neolithic painted pottery and ceramic figurines with Y-chromosome lineages". Antiquity. 76 (293): 707 ... "Y-chromosome and mtDNA polymorphisms in Iraq, a crossroad of the early human dispersal and of post-Neolithic migrations" (2003 ... Nebel A, Filon D, Brinkmann B, Majumder PP, Faerman M, Oppenheim A (Nov 2001). "The Y Chromosome Pool of Jews as Part of the ... In Y-chromosome phylogenetics, subclades are the branches of haplogroups. These subclades are also defined by single nucleotide ...
Our inter-species chromosome painting results unequivocally illustrate the dynamic reshuffling of ancestral chromosomes among ... Chromosome repatterning in three representative parrots (Psittaciformes) inferred from comparative chromosome painting ... The cross-species chromosome painting results show that, unlike in many other avian karyotypes with high homology to chicken ... Chromosome painting with DNA probes derived from the flow-sorted macrochromosomes (1-10) of chicken (Gallus gallus, GGA) has ...
Comparative chromosome painting of pronghorn (Antilocapra americana) and saola (Pseudoryx nghetinhensis) karyotypes with human ... Pronghorn; Antilocapra americana; Saola; Pseudoryx nghetinhensis; Comparative cytogenetics; Pecora; Phylogeny; Chromosome ...
... Ricky Critcher rc at mole.bio.cam.ac.uk Mon Feb 5 03:13:58 EST 1996 *Previous message: Mouse ... Alternativly you could make your own if you can get hold of some monochromosomal mouse hybrids and pcr the mouse chromosome. * ... Cambio (UK) sell mouse paints which are labelled with Cy 3, biotin, or FITC. ...
... Bernd Koelsch ttu3a0 at aixrs1.hrz.uni-essen.de Tue Jun 13 19:04:06 EST 1995 *Previous message: rat gene ... Is there anyone who knows wher I can get such paints?? Bernd K lsch Cell-biology 45147 Essen, Germany e-mail: ttu3a0 at aixrs1. ...
Chromosome painting for plant biotechnology.. Kato A1, Lamb JC, Albert PS, Danilova T, Han F, Gao Z, Findley S, Birchler JA. ... This chapter details the labeling procedures and chromosome preparation techniques used to produce high-quality FISH signals on ... Fluorescence in situ hybridization (FISH) is an invaluable tool for chromosome analysis and engineering. The ability to ...
Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize. Akio Kato, ... Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize ... Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize ... Chromosome painting using repetitive DNA sequences as probes for somatic chromosome identification in maize ...
PCR products from the isolated chromosomes were used in an in situ probe mixture for painting the Y chromosome. The ... Role of Y chromosome in sex determination; Sequencing of the euchromatin of the Y chromosome; Degree and patterns of divergence ... Home » Microdissection and painting of the Y chromosome in spinach ( Spinacia oleracea) ... For the first time, the largest spinach chromosome was confirmed to be a sex chromosome at the molecular level. ...
Gribble S, Ng BL, Prigmore E et al. (2004) Chromosome paints from single copies of chromosomes. Chromosome Res 12: 143-151. ... Generation of Chromosome Painting Probes from Single Chromosomes by Laser Microdissection and Linker-Adaptor PCR. ... Deng HX, Yoshiura K, Dirks RW et al. (1992) Chromosome-band-specific painting: chromosome in situ suppression hybridization ... Langer S, Kraus J, Jentsch I et al. (2004) Multicolor chromosome painting in diagnostic and research applications. Chromosome ...
What is chromosome painting? Meaning of chromosome painting medical term. What does chromosome painting mean? ... Looking for online definition of chromosome painting in the Medical Dictionary? chromosome painting explanation free. ... chromosome painting fluorescent in situ hybridization.. Ph1 chromosome (Philadelphia chromosome) an abnormality of chromosome ... chromosome painting. Also found in: Dictionary, Thesaurus, Encyclopedia. chromosome. [kro´mo-sōm] in animal cells, a structure ...
... differing by only three chromosome pairs. Nearly all chromosomes of PDI and PHA were conserved in toto, except for chromosome ... Comparative analysis using G-banding and chromosome painting show that the karyotypic complement of TSA is highly rearranged ... Homologous chromosomes Is the Subject Area "Homologous chromosomes" applicable to this article? Yes. No. ... Chromosome pairs Is the Subject Area "Chromosome pairs" applicable to this article? Yes. No. ...
Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chromosom Cancer 4:257-263 ... Comparative painting reveals strong chromosome homology over 80 million years of bird evolution. Chromosome Res 7:289-295PubMed ... Rens W, Fu B, OBrien PCM, Ferguson-Smith MA (2006) Cross-species chromosome painting. Nat Protoc 1:783-790PubMedCrossRefGoogle ... Karyotype relationships between four distantly related marsupials revealed by reciprocal chromosome painting. Chromosome Res 7: ...
RxFISH painting of chromosomes. Genetics as it applies to evolution, molecular biology, and medical aspects. ... but, is it all doable in the living cell? so that chromosomes can sit there? also Is it easy to put back condensed chromosomes ... does RxFISH painting, disrupts the dna? so that it would cause damage to the genetic material? I read that it is some sort of ...
Cross-species chromosome painting was used to delineate regions of homology between Loxodonta africana (2n = 56), Procavia ... Cross-species chromosome painting was used to delineate regions of homology between Loxodonta africana (2n = 56), Procavia ... Chromosome painting among Proboscidea, Hyracoidea and Sirenia: Support for Paenungulata (Afrotheria, Mammalia) but not ... Chromosome painting among Proboscidea, Hyracoidea and Sirenia: Support for Paenungulata (Afrotheria, Mammalia) but not ...
... suggested that B chromosomes in the grasshopper ,i& ... Chromosome paints from single copies of chromosomes. Chromosome ... Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria. Chromosome Res 17:11-18 (2009). External ... X chromosome painting in Microtus: origin and evolution of the giant sex chromosomes. Chromosome Res 12:767-776 (2004). ... Microdissection and Chromosome Painting of X and B Chromosomes in the Grasshopper Eyprepocnemis plorans. Teruel M.a · Cabrero J ...
The paint probes can be used as chromosome counting probes. Chromosome Paint Probes can be used on metaphase or interphase ... CABR™ Chromosome Probes ▼ Whole Chromosome Painting ProbesCentromere ProbesSubtelomere Specific ProbesSatellite Enumeration ...
Chromosome painting and arrangement of interphase chromosome territories in Arabidopsis thaliana, Verfasser: Pecinka, Ales, ... Chromosome painting, utilizing tiling a path of bacterial artificial chromosome (BAC) clones, has been established for model ... Chromosome painting and arrangement of interphase chromosome territories in Arabidopsis thaliana / von Ales Pecinka. ... Using chromosome painting, arrangement and potential dynamics of Arabidopsis interphase chromosome territories (CTs) were ...
Furthermore, the probe painted an autosomal region in seven species from our sample with characterized sex chromosomes, and ... Strong conservation of the bird Z chromosome in reptilian genomes is revealed by comparative painting despite 275 million years ... We used chromosome-specific DNA from flow-sorted chicken (Gallus gallus) Z sex chromosomes as a probe in cross-species ... The avian Z chromosome synteny is, therefore, conserved albeit it is not a sex chromosome in these squamate species.. ...
Chromosome painting with libraries from chromosomes 1 and X excluded a t(X;1) translocation, but failed to detect a second ... Through reverse chromosome painting, an unbalanced der(1), t(1;10) (q44;q26) translocation could be detected. This is the third ... Kallmann syndrome in a boy with a t(1;10) translocation detected by reverse chromosome painting. ... Kallmann syndrome in a boy with a t(1;10) translocation detected by reverse chromosome painting. ...
of Multidirectional chromosome painting substantiates the occurrence of extensive genomic reshuffling within Accipitriformes ... of Multidirectional chromosome painting substantiates the occurrence of extensive genomic reshuffling within Accipitriformes. ... Accipitriformes Fluorescent in situ hybridization Multidirectional painting Chromosomal rearrangements Chromosome-based ...
A comprehensive range of human Whole Chromosome Painting probes available in either a red or a green fluorophore. Request a ... Cytocell offers a comprehensive range of human Whole Chromosome Painting probes available in the Aquarius® liquid format. ... Whole Chromosome Painting Probes IFU (LPP xxR/G) Whole Chromosome Painting Probes IFU (LPP xxR/G) ...
Utilisation of Chromosome Painting as a Complementary Tool for Introgression Analysis and Chromosome Identification in Coffee ... Utilisation of Chromosome Painting as a Complementary Tool for Introgression Analysis and Chromosome Identification in Coffee ...
We also offer a very wide range of chromosome painting probes from Cytotest™ which whole chromosomes, chromosome arms and ... Cambios Star*FISH paints are highly chromosome-specific and provide robust and even coverage of individual chromosomes, unlike ... terminal bands, sub-telomeric probes, chromosome counting probes, locus probes and oncology probes. ... Chromosome Painting. Cambios Star*FISH paints are highly chromosome-specific and provide robust and even coverage of ...
Cytocells Whole Chromosome Painting Probes consist of libraries of DNA sequences derived from flow-sorted chromosomes. These ... Cytocell offers chromosome painting probes in the Aquarius® liquid format and on a Chromoprobe Multiprobe® device, where FISH ... Applications include analysis of chromosome partners involved in translocations; identification of the chromosome of origin of ... contain sequences stretching over the entire length of the chromosome and provide superior coverage of each human chromosome. ...
... cambio.co.uk/1/1/applications/methods/chromosome-painting/human/ target=_blank> click here.,/a>,/span>,/strong>,/em> ,br ... you are looking for a range of human chromosome specific probes , including whole chromosome and centromeric and telomeric then ... p>,p>The NEW range from Cytotest currently includes the Mouse X and Mouse Y whole chromosome probes. These are available with ... p>Our range of mouse specific chromosome paint includes the Starfish Pan-centromeric probes, available with either a directly ...
The use of chromosome painting is a modern way of genomic comparison on the cytological level, and will clarify the intense ... and these probes were used in reciprocal chromosome painting. The hybridization of the Phyllostomus probes on the Carollia ... Whole chromosome probes of species were produced as a tool for evolutionary studies in this family from two species from ... Many chromosome rearrangements have occurred during the divergence of these two genera. The sequence of events suggested a ...
  • Chromosome painting with DNA probes derived from the flow-sorted macrochromosomes (1-10) of chicken (Gallus gallus, GGA) has been used to identify and distinguish the homoeologous chromosomal segments in three species of parrots, i. e. (kent.ac.uk)
  • In contrast, the smaller GGA macrochromosomes 6, 7 and 8 displayed a complex hybridization pattern: two or three of these macrochromosomes were found to be contiguously arranged on a single chromosome in all three parrot species. (kent.ac.uk)
  • The ten GGA macrochromosome paints unequivocally recognize 14 to 16 hybridizing regions delineating the conserved chromosomal segments for the respective chicken macrochromosomes in these representative parrot species. (kent.ac.uk)
  • Among the larger GGA macrochromosomes (1-5), chromosomes 1 and 4 are conserved on two chromosomes in all three species. (kent.ac.uk)
  • If your parents come from different genetic backgrounds, then you may be able to guess which chromosomes you inherited from which of your parents just by looking at the ancestries painted on each chromosome. (23andme.com)
  • The confidence slider on the Chromosome Painting allows you to explore our estimates of your genetic ancestry at different probability cutoffs. (23andme.com)
  • It contains most of the cell's genetic material (DNA), organized as multiple long linear DNA molecules in complex with a large variety of proteins, such as histones, to form chromosomes. (wikibooks.org)
  • Here we use cytological maps of crossing over based on recombination nodules (RNs) to predict the physical position of genetic markers on each of the 10 chromosomes of maize. (genetics.org)
  • citation needed] Wilhelm Roux suggested that each chromosome carries a different genetic load. (wikipedia.org)
  • A genetic test is the analysis of human deoxyribonucleic acid (DNA), ribonucleic acid (RNA), chromosomes, and proteins to detect heritable disease-related genotypes, mutations, phenotypes, or karyotypes (standard pictures of the chromosomes in a cell) for the purposes of diagnosis, treatment, and other clinical decision making. (encyclopedia.com)
  • in 1902, the Boveri-Sutton Chromosome Theory unified the genetic laws of Mendelian inheritance with the physical structures of chromosomes observed in cells? (thefullwiki.org)
  • Pallister-Killian syndrome is a rare congenital genetic disorder that cannot be detected through prenatal blood tests because it occurs only in the chromosomes of skin cells ? (thefullwiki.org)
  • microchromosomes are very tiny gene -rich chromosomes which are a typical genetic component in birds , and some groups of non-mammalian animals? (thefullwiki.org)
  • That's quite a role change for the once-lowly Y. For years, researchers viewed the Y chromosome as a genetic wasteland, good for little else but serving up a male reproductive system. (csmonitor.com)
  • He's known by the fly world as the scientist who discovered cosuppression in Drosophila , by the plant world as the cytogeneticist who figured out how to paint, break, and regrow plant chromosomes, and by ∼4500 students who have taken genetics at "Mizzou" as the professor who teaches Punnett squares and Mendelian inheritance while dressed as the Father of Genetics himself. (plantcell.org)
  • The secret, they say, is to 'paint' the plant chromosomes before trying to understand their origins. (newscientist.com)
  • Comments on the 'Sex-determining Region on the Y chromosome' gene in Great Britain. (ebscohost.com)
  • In most mammals, male development is triggered by the transient expression of the Y-chromosome gene, Sry, which initiates a cascade of gene interactions ultimately leading to the formation of a testis from the indifferent fetal gonad[SUP1- (ebscohost.com)
  • This image shows a probe for the Xist gene, on the X chromosome. (washington.edu)
  • 4 The risk of an abnormal phenotype associated with such markers is relatively high with Crolla 4 estimating the risk to be approximately 28% for rings derived from non-acrocentric chromosomes and approximately 7% from acrocentric chromosomes. (bmj.com)
  • Macrotus californicus chromosome paints will be a valuable tool for documenting the pattern of karyotypic evolution within Phyllostomidae radiation. (biomedcentral.com)
  • Integration of empirical data with proposed models of chromosome evolution was performed to understand the probable conditions for Tonatia 's karyotypic evolution. (biomedcentral.com)
  • The trajectory of reorganization of chromosome blocks since the common ancestor of Glossophaginae and Phyllostominae subfamilies suggests that multiple tandem fusions, as well as disruption and fusions of conserved phyllostomid chromosomes were major drivers of karyotypic reshuffling in Tonatia . (biomedcentral.com)
  • Because of their high levels of karyotypic variation, phyllostomid bats are an ideal group to investigate the mechanisms responsible for chromosome evolution and stasis. (biomedcentral.com)
  • However, the identification of somatic chromosomes has been difficult because the highly condensed chromatin structure conceals the fine details that are used for chromosome identification at the pachytene stage, such as cytologically observable knobs, heterochromatic regions, arm ratios, and total chromosome length ( 5 ). (pnas.org)
  • Some use the term chromosome in a wider sense, to refer to the individualized portions of chromatin in cells, either visible or not under light microscopy. (wikipedia.org)
  • Chromatin The network of chromosomes, histones, and other proteins found in the eukaryotic nucleus during interphase. (thefullwiki.org)
  • To this end, we attempted bacterial artificial chromosome (BAC) detection on maize chromosomes. (pnas.org)
  • Chromosome painting, utilizing tiling a path of bacterial artificial chromosome (BAC) clones, has been established for model plant Arabidopsis thaliana. (uni-halle.de)
  • The in‐situ hybridisation of the deoxyribonucleic acid (DNA) cloned into bacterial artificial chromosome overcomes the limitations of chromosome painting permitting the reconstruction of ancestral marker order along the single chromosome and has revealed that centromeres can reposition during evolution. (els.net)
  • Birds, unlike squamates, have karyotypes that are typified by the presence of a number of very small chromosomes. (springer.com)
  • Chromosome painting on three different DFTD tumour strains determined the origins of marker chromosomes and provided a general overview of the rearrangement in DFTD karyotypes. (edu.au)
  • In this study, size and GC content of all chromosomes are measured from the flow karyotypes of GGA, TSC and CNI. (royalsocietypublishing.org)
  • Results indicated that patients with r(1) chromosomes consisting of 1q12 heterochromatin and short arm pericentric euchromatin which extends to at least the BAC923C6 were associated with a normal or mild phenotype. (bmj.com)
  • Other hypotheses focus on differences in chromosome cores and cohesins, SC structure in heterochromatin compared to euchromatin, underrepresentation of heterochromatin in SC length, and interference with synapsis (SC formation) in heterochromatin. (genetics.org)
  • The colors in your Chromosome Painting tell you about your Ancestry Composition, but you can learn even more by looking at the number and length of the DNA segments assigned to each ancestry. (23andme.com)
  • The number to the right of chromosomes or chromosome segments also indicates human homology. (els.net)
  • These are the result of many independent coiling events happening simultaneously along the entire length of the chromosome. (umanitoba.ca)
  • For example, idic(15) chromosomes can be grouped into several categories with clustering of 15p breakpoints. (bmj.com)
  • Our data demonstrate a high level of reuse of breakpoints among muroid rodents, further supporting the "Fragile Breakage Model" of chromosome evolution. (deepdyve.com)
  • The amplified products from the Y chromosome were identified using the male-specific marker T11A. (ebscohost.com)
  • This was based on published studies of randomly ascertained supernumerary marker chromsomes, not exclusively small ring chromosomes. (bmj.com)
  • Study of the maize ( Zea mays L.) somatic chromosomes (2 n = 20) has been difficult because of a lack of distinguishing characteristics. (pnas.org)
  • If you were to paint the genomes of people in modern-day Maya, for example, you would use a mixed palette with colours from Spanish-like, West African and Native American DNA. (medicalxpress.com)
  • the chromosome theory of heredity was developed. (wikibooks.org)
  • In a series of experiments beginning in the mid-1880s, Theodor Boveri gave the definitive demonstration that chromosomes are the vectors of heredity. (wikipedia.org)
  • In his famous textbook The Cell in Development and Heredity, Wilson linked together the independent work of Boveri and Sutton (both around 1902) by naming the chromosome theory of inheritance the Boveri-Sutton chromosome theory (the names are sometimes reversed). (wikipedia.org)
  • The first procedure to identify maize meiotic chromosomes was developed by McClintock ( 1 ), and the method was refined and detailed by Longley ( 2 ) and Rhoades ( 3 ) in the middle of the 20th century. (pnas.org)
  • He describes techniques for shuttling entire biochemical pathways into cells using artificial chromosomes, methods for enhancing transformation efficiencies and enabling bioengineering in recalcitrant crops, and developing a fast-flowering maize line that's poised to beat Arabidopsis in a seed-to-seed race. (plantcell.org)
  • It was there that he started "playing around with the chromosome arms of maize. (plantcell.org)
  • He performed allozyme assays on maize trisomy lines to look at changes in enzyme expression in response to chromosome dosage. (plantcell.org)
  • Related observations indicate that differences in mitotic and meiotic chromosomes may affect the relative cytological distance between markers in plants as well ( S tack 1984 ). (genetics.org)
  • Telomere shortening in these mice is accompanied by an increase in the number of chromosome ends without detectable telomeres and in the frequency of chromosome fusions ( 15 )( 16 ). (rupress.org)
  • Although chromosome painting is used throughout biology, especially in medicine, molecular biology and genetics, some of the most dramatic results are coming from plant science. (newscientist.com)
  • Ezaz T, Sarre SD, O'Meally D, Graves JAM, Georges A (2010) Sex chromosome evolution in lizards: independent origins and rapid transitions. (springer.com)
  • A second team, led by University of Laussane researcher Henrik Kaessmann, analyzed the Y chromosomes from 15 mammals across a wider range of mammals and traced the origins of the Y chromosome as far back as 180 million years ago, when placental mammals and marsupials diverged. (csmonitor.com)
  • Two patients showed molecular cytogenetic results which were compatible with non-contiguous chromosome 1 euchromatin. (bmj.com)
  • In addition, the small ring chromosomes may contain variable regions of euchromatin originating from the pericentromeric short or long arms or contain discontinuous regions of euchromatin 5 necessitating detailed molecular cytogenetic characterisation. (bmj.com)
  • If this is correct, then there could be a subgroup of patients with accessory ring chromosomes derived from the same chromosome with similar regions of euchromatin and therefore a characteristic phenotype. (bmj.com)
  • Attention is paid to their length, the position of the centromeres , banding pattern, any differences between the sex chromosomes , and any other physical characteristics. (wikipedia.org)
  • Ideally, detailed investigation of the chromosome architecture of an organism and related groups, placed within a phylogenetic context, is required. (biomedcentral.com)