A technique for causing a targeted loss of molecular function from REACTIVE OXYGEN SPECIES that are formed by the illumination of dyes placed in the immediate vicinity of the target molecule.
That portion of the electromagnetic spectrum in the visible, ultraviolet, and infrared range.
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
A carotenoid constituent of visual pigments. It is the oxidized form of retinol which functions as the active component of the visual cycle. It is bound to the protein opsin forming the complex rhodopsin. When stimulated by visible light, the retinal component of the rhodopsin complex undergoes isomerization at the 11-position of the double bond to the cis-form; this is reversed in "dark" reactions to return to the native trans-configuration.
Agents that interact with TUBULIN to inhibit or promote polymerization of MICROTUBULES.
Nocodazole is an antineoplastic agent which exerts its effect by depolymerizing microtubules.
Slender, cylindrical filaments found in the cytoskeleton of plant and animal cells. They are composed of the protein TUBULIN and are influenced by TUBULIN MODULATORS.
The basic cellular units of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the NERVOUS SYSTEM.
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
A ubiquitous phosphoprotein that serves as an intracellular substrate for a variety of SIGNAL TRANSDUCTION PATHWAYS. PHOSPHORYLATION of stathmin occurs during CELL CYCLE progression, and stathmin functions as a microtubule-destabilizing protein that promotes MICROTUBULE depolymerization during INTERPHASE and late MITOSIS. Stathmin is expressed at very high levels in a variety of human CANCERS.
A major alkaloid from Colchicum autumnale L. and found also in other Colchicum species. Its primary therapeutic use is in the treatment of gout, but it has been used also in the therapy of familial Mediterranean fever (PERIODIC DISEASE).
The combination of genetic and optical methods in controlling specific events with temporal precision in targeted cells of a functioning intact biological system.
A province of eastern Canada. Its capital is Quebec. The region belonged to France from 1627 to 1763 when it was lost to the British. The name is from the Algonquian quilibek meaning the place where waters narrow, referring to the gradually narrowing channel of the St. Lawrence or to the narrows of the river at Cape Diamond. (From Webster's New Geographical Dictionary, 1988, p993 & Room, Brewer's Dictionary of Names, 1992, p440)
A publication issued at stated, more or less regular, intervals.
Compounds that contain the triphenylmethane aniline structure found in rosaniline. Many of them have a characteristic magenta color and are used as COLORING AGENTS.
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
A product of hard secondary xylem composed of CELLULOSE, hemicellulose, and LIGNANS, that is under the bark of trees and shrubs. It is used in construction and as a source of CHARCOAL and many other products.
"The business or profession of the commercial production and issuance of literature" (Webster's 3d). It includes the publisher, publication processes, editing and editors. Production may be by conventional printing methods or by electronic publishing.
The premier bibliographic database of the NATIONAL LIBRARY OF MEDICINE. MEDLINE® (MEDLARS Online) is the primary subset of PUBMED and can be searched on NLM's Web site in PubMed or the NLM Gateway. MEDLINE references are indexed with MEDICAL SUBJECT HEADINGS (MeSH).
Publications in any medium issued in successive parts bearing numerical or chronological designations and intended to be continued indefinitely. (ALA Glossary of Library and Information Science, 1983, p203)
The circulation of blood through the BLOOD VESSELS of the BRAIN.
The tendency of a gas or solute to pass from a point of higher pressure or concentration to a point of lower pressure or concentration and to distribute itself throughout the available space. Diffusion, especially FACILITATED DIFFUSION, is a major mechanism of BIOLOGICAL TRANSPORT.
An element with atomic symbol O, atomic number 8, and atomic weight [15.99903; 15.99977]. It is the most abundant element on earth and essential for respiration.
The flow of BLOOD through or around an organ or region of the body.
A value equal to the total volume flow divided by the cross-sectional area of the vascular bed.
Unstable isotopes of xenon that decay or disintegrate emitting radiation. Xe atoms with atomic weights 121-123, 125, 127, 133, 135, 137-145 are radioactive xenon isotopes.
Experimentation on STEM CELLS and on the use of stem cells.
One of three principal openings in the SUBARACHNOID SPACE. They are also known as cerebellomedullary cistern, and collectively as cisterns.
A fungal metabolite which is a macrocyclic lactone exhibiting a wide range of antibiotic activity.
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
Glycoside hydrolases that catalyze the hydrolysis of alpha or beta linked MANNOSE.
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
Therapy using oral or topical photosensitizing agents with subsequent exposure to light.
Drugs that are pharmacologically inactive but when exposed to ultraviolet radiation or sunlight are converted to their active metabolite to produce a beneficial reaction affecting the diseased tissue. These compounds can be administered topically or systemically and have been used therapeutically to treat psoriasis and various types of neoplasms.
Copies of a work or document distributed to the public by sale, rental, lease, or lending. (From ALA Glossary of Library and Information Science, 1983, p181)
The purified component of HEMATOPORPHYRIN DERIVATIVE, it consists of a mixture of oligomeric porphyrins. It is used in photodynamic therapy (HEMATOPORPHYRIN PHOTORADIATION); to treat malignant lesions with visible light and experimentally as an antiviral agent. It is the first drug to be approved in the use of PHOTODYNAMIC THERAPY in the United States.
A rare autosomal recessive disease characterized by the deposition of copper in the BRAIN; LIVER; CORNEA; and other organs. It is caused by defects in the ATP7B gene encoding copper-transporting ATPase 2 (EC 3.6.3.4), also known as the Wilson disease protein. The overload of copper inevitably leads to progressive liver and neurological dysfunction such as LIVER CIRRHOSIS; TREMOR; ATAXIA and intellectual deterioration. Hepatic dysfunction may precede neurologic dysfunction by several years.
Medical complexes consisting of medical school, hospitals, clinics, libraries, administrative facilities, etc.
The affective response to an actual current external danger which subsides with the elimination of the threatening condition.
Complex mental function having four distinct phases: (1) memorizing or learning, (2) retention, (3) recall, and (4) recognition. Clinically, it is usually subdivided into immediate, recent, and remote memory.
A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Almond-shaped group of basal nuclei anterior to the INFERIOR HORN OF THE LATERAL VENTRICLE of the TEMPORAL LOBE. The amygdala is part of the limbic system.
Learning that takes place when a conditioned stimulus is paired with an unconditioned stimulus.
An agency of the PUBLIC HEALTH SERVICE concerned with the overall planning, promoting, and administering of programs pertaining to maintaining standards of quality of foods, drugs, therapeutic devices, etc.
The order of amino acids as they occur in a polypeptide chain. This is referred to as the primary structure of proteins. It is of fundamental importance in determining PROTEIN CONFORMATION.
Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories.
Disturbances in registering an impression, in the retention of an acquired impression, or in the recall of an impression. Memory impairments are associated with DEMENTIA; CRANIOCEREBRAL TRAUMA; ENCEPHALITIS; ALCOHOLISM (see also ALCOHOL AMNESTIC DISORDER); SCHIZOPHRENIA; and other conditions.
The part of CENTRAL NERVOUS SYSTEM that is contained within the skull (CRANIUM). Arising from the NEURAL TUBE, the embryonic brain is comprised of three major parts including PROSENCEPHALON (the forebrain); MESENCEPHALON (the midbrain); and RHOMBENCEPHALON (the hindbrain). The developed brain consists of CEREBRUM; CEREBELLUM; and other structures in the BRAIN STEM.
Directed modification of the gene complement of a living organism by such techniques as altering the DNA, substituting genetic material by means of a virus, transplanting whole nuclei, transplanting cell hybrids, etc.

Selective fluorophore-assisted light inactivation of voltage-gated calcium channels. (1/5)

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A trifluoroacetic acid-labile sulfonate protecting group and its use in the synthesis of a near-IR fluorophore. (2/5)

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Bringing optogenetics to the synapse. (3/5)

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Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). (4/5)

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SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. (5/5)

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Journal Club 2012-2013 Date Journal Article for Discussion Nov. 29, 2013 Sleep drives metabolite clearance from the adult brain. Xie et al. Science 2013 342(6156):373-7 Nov. 8, 2013 Declines in Drp1 and Parkin expression underlie DNA damage-induced changes in mitochondrial length and neuronal death. Wang et al. J Neurosci 2013 33(4): 1357-65 Nov. 1, 2013 BLA to vHPC inputs modulate anxiety-related behaviors. Felix-Ortiz et al. Neuron 2013 79(4): 658-64 Oct. 25, 2013 Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Lin et al.Neuron 2013 79(2):241-53 June 21, 2013 Permanent Genetic Access to Transiently Active Neurons via TRAP: Targeted Recombination in Active Populations. Guenthner et al. Neuron 2013 78(5):773-84 May 24, 2013 Making waves: initiation and propagation of corticothalamic Ca2+ waves in vivo. Stroh et al. Neuron 2013 77(6):1136-50 May 17, 2013 The cell-autonomous role of excitatory
Date: Fri, 03 Dec 1999 16:44:06 -0800 From: Alison, Colin, Scott Birdwell ,defalcos at insync.net, Subject: ramblings on Nottingham, AHA, & mills Paul Shick was wondering why his lag time with Nottingham yeast was so long. We at DeFalcos have sold this yeast for a number of years with little or no trouble with lag times, whatsover. However, we dont usually deal with the puny little 5 gram packets. We buy in bulk and package in 12 gram packets for 5 gallon batches. They really take off! What little negative feedback weve received is from customers whove bought supplies from competitors (Tsk! Tsk!) and gotten the 5 gram packets. I know this doesnt make sense. You would think the professionally packaged yeast would hold up better, but this has not been our experience. I do have one question for Paul and one observation. The question is regarding your re-hydration: You said you re-hydrated the yeast and even took pains to prevent thermal shock, but you didnt make any comments on the activity ...
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It is emphasized here that you should input the fit function value, but not the model fit chi-square value, of the baseline model in the BASEFUNC= option. For all estimation methods except the full information maximum likelihood (FIML) method in PROC CALIS, the model fit chi-square values are some multiples of the fit function values. See the section Estimation Criteria for the definitions of the various fit functions that are assumed by the BASEFUNC= option. Alternatively, it might be easier to use the BASEFIT= option to specify the SAS data set that contains the baseline model fit information. Such a SAS data set is typically created by using the OUTFIT= option in the PROC CALIS fitting of your customized baseline model. See the BASEFIT= option for details. However, the BASEFIT= option is ignored if you also specify the BASEFUNC= option. Notice that the specified values in the BASEFUNC= option do not affect the computation of all fit indices. Mainly, they affect the incremental fit indices, ...
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Cali loved to talk to us. When we would come home from work, Cali would greet us with woo-woo-woo-woo (see the picture to the left). She also was very protective of us. Especially Holly. If I wasnt in the house at night because I was out late or because I was away, Cali would be on duty. She would usually put herself halfway between Holly and the front door. And she growled at people that even thought about walking near our house. And when Holly or I were injured (happens a lot at our house), she would stand at the bathroom door ready to bark if we fell in the shower. Along those lines, one of my proudest moments in life was when I was ready to protect her. We were out for a walk one night, just her and I, when a rottweiler without a lease ran away from its owner towards us growling and barking like it was in kill mode. Without thinking, I choked up on Calis leash and put myself between Cali and the approaching dog while yelling at the owner to get control of their @#$%ing dog. Luckily, ...
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A world drug capital struggles to get a life. LaFranchi, Howard // Christian Science Monitor;3/9/95, Vol. 87 Issue 71, p6 Features the city Cali in Colombia and the effect of drug on it. Information on a Cali resident, Adelinda Wailis who is unemployed and has lost a friend and the father of her son; Factors leading to the increasing drug abuse and violence in Cali; Effort of the Cali authorities to create human... ...
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My mini dachshunds face appears bumpy and she is hyperactive, Cali 6.5 months, she it itching a lot - we just came - Answered by a verified Dog Veterinarian
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Stand on the right side of your Bosu Ball on your left leg with your inner core muscles engaged. Slowly bend down at your hips pointing your left finger to a spot on the floor in the nine Oclock position on the face of a clock. Then straighten back up to standing pushing through your entire foot with a focus on your heel. Bend back down pointing to the floor in the 10 Oclock position and stand back up again. Continue to repeat this for the eleven, twelve, one, two and three Oclock positions. Then perform a lateral hop onto the Bosu Ball and then onto the floor to the left side, and repeat the one leg squats from nine to three Oclock. Then repeating your second set on the right & left sides of the Bosu; you can now go backwards and point down to the floor starting from three to nine Oclock. Remember that as you bend at your knee, you will be bending even more at your hips and you want to keep you knee over your ankle and aligned with your second toe. Perform 2 sets on each side for each leg ...
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Here she is: recovering from a full-face skunk hit. At the time of this photo the worst was over for Cali the Black Laborador —- in spite of the somewhat mournful look you see in her portrait --- and she would soon be able to sleep in the cabin.
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El nue-vo vehícu-lo en-tra a un mer-ca-do ca-da vez más de-sa-rro-lla-do, con una ca-pa-ci-dad pa-ra cua-tro per-so-nas y una mecánica sen-ci-lla
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Its now legal in Cali to smoke recreational pot. I grew this pot using my vitamin enriched urine. Someone close to me smoked my plant and said it was really good feces. Youd think that being in B ...
Supernova Sonata by Alex Parker From April, 2003 until August, 2006, the Canada-France-Hawaii Telescope watched four parts of the sky as often as possible. Armed with the largest digital camera in the known universe, CFHT monitored these four fields for a special type of supernova (called Type Ia) which are created by the thermonuclear detonation of one or more white-dwarf stars. Each supernova is assigned a note to be played: The volume of the note is determined by the distance to the supernova, with more distant supernova being quieter and fainter. The pitch of the note was determined by the supernovas stretch, a property of how the supernova brightens and fades. Higher stretch values played higher notes. The pitches were drawn from a Phrygian dominant scale. The instrument the note was played on was determined by the properties of the galaxy which hosted each supernova. Supernovae hosted by massive galaxies are played with a stand-up bass, while supernovae hosted by less massive galaxies ...
Up until now, though, that hasnt been a terribly urgent goal; the time-scale of galactic events is immense, usually so long that the average scientist quickly gives up on the idea of seeing the universes most dramatic displays within their lifetime. That attitude may change with respect to galactic supernovae now, thanks to this teams finding that the Milky Way is virtually certain to host a supernova within the next 50 years. Given those sorts of odds, the team argues, it would be silly not to start investing right away.. Though it is nearby within the context of the entire universe, even a galactic supernova is happening very, very far away; the probability of a supernova strong and close enough to be visible to the naked eye is closer to 1 in 5. The chance of a sky-brightening event that outshines all other objects in the sky, something like the incredible event observed by Kepler in 1604, is more like 1 in 20.. This sort of work can be difficult to justify to funding boards since it could ...
Supernova in the Galaxy M65 on 23 March, image taken with iTelescope T04, 5x120 second exposures stacked and registered in ImageJ then a SUM Z-project applied, followed by contrast adjustment and colour inversion. Supernova indicated by black bars (click to embiggen ...
In the era of large astronomical surveys, photometric classification of supernovae (SNe) has become an important research field due to limited spectroscopic resources for candidate follow-up and classification. In this work, we present a method to photometrically classify type Ia … - 1608.05423
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Robert Kirshner. The above plot shows light curves for five different supernovae. The brightness of each supernova (in the units of magnitude favored by astronomers) is plotted as a function of time. Note that the light from the brighter supernovae (purple and blue curves) decreases more slowly over time than does light from the dimmer supernovae (the black curve). This subtle difference allows astronomers to calibrate the brightness of the type Ia supernovae and use them as standard candles. (Unit: 11). ...
Plasmid pK237.U6-Chimeric-CAG-loxP-stop-loxP-hspCas9 (Supernova) from Dr. Takuji Iwasatos lab contains the insert loxP-stop-loxP and is published in Sci Rep. 2016 Oct 24;6:35747. doi: 10.1038/srep35747. This plasmid is available through Addgene.
Supernova is a modern scripting language for Microsoft-Windows released as Freeware; Author: Mahmoud Samir Fayed; Updated: 13 Apr 2010; Section: Win32/64 SDK & OS; Chapter: Platforms, Frameworks & Libraries; Updated: 13 Apr 2010
EVGA recently introduced their new flagship SuperNOVA unit with 1600 W capacity and 80 Plus Gold efficiency. According to their description, this PSU is the ultimate tool for any highly overclocked system, and in this review, we plan to push it beyond its limits to see how it fares.
Properties of the ultraviolet flux of type Ia supernovae: an analysis with synthetic spectra of SN 2001ep and SN 2001eh - Astrophysics. . Biblioteca virtual para leer y descargar libros, documentos, trabajos y tesis universitarias en PDF. Material universiario, documentación y tareas realizadas por universitarios en nuestra biblioteca. Para descargar gratis y para leer online.
Supernova is a modern scripting language for Microsoft-Windows released as Freeware; Author: Mahmoud Samir Fayed; Updated: 13 Apr 2010; Section: Win32/64 SDK & OS; Chapter: Platforms, Frameworks & Libraries; Updated: 13 Apr 2010
- Made in the UK- 50ml Shortfill- 70VG/30PG- Contains natural cannabis terpenesGelato BrownieIn this terpenes e-liquid, weve married the sweet and earthy taste of gelato to the opulent rich indulgence of sticky fudge brownie.Amnesia MangoUnlike mango kush, this terpenes e-liquid by Cali Greens is a true Kesar mango fl
Informe del Programa de Frijol para la Zona Andina: taller de planificacion de la segunda fase. Cali, Colombia, julio 30 a agosto 3, 1990 ...
The Cali Ruby/Rails Meetup Group es una comunidad abierta de desarrolladores de todos los niveles (básico, intermedio y avanzado) interesados en aprender y compartir experiencias con Ruby, Ruby on Rai
Na početku smo čuli dva predavanja, oba na temu nepravilnosti na koži i problema koje akne izazivaju kod osoba koje imaju problema sa njima. Prvo predavanje obuhvatalo je psihološki aspekt ovog problema, i to kako stres utiče na pogoršanje bubuljica, ali i sam stres i probleme koje bubuljice izazivaju kod osoba koje se sa njima bore. Iz tog predavanja morala sam da izdvojim ovu rečenicu sa kojom je predavanje počelo i koja se nalazi na slici iznad ...
EVGA power supplies have been scoring well in our lab tests now for some time. Their partnership with design manufacturers Seasonic and SuperFlower is in no
Howard Greenstein interviewed David Isenberg at the Personal Democracy Forum 2009. In this interview, David talks about his take on Change Networks, and notes how, without the stupid network of packets, wed be missing all sorts of innovation.
Παρουσίαση της Διδακτορικής Διατριβής του Salvatore Cali (1 Ιουνίου 2019). Παρουσίαση της Διδακτορικής Διατριβής του Simone Bacchio (31 Μαΐου 2019). Παρουσίαση της Διδακτορικής Διατριβής της Aurora Scapellato (30 Μαΐου 2019). Παρουσίαση της Διδακτορικής Διατριβής του Γιώργου Χαρίτου (10 Μαΐου 2019). Παρουσίαση της Διδακτορικής Διατριβής του Κυριάκου Κυριάκου (10 Μαΐου 2019). Παρουσίαση της Διδακτορικής Διατριβής του Γρηγόρη Σπανούδη (9 Μαΐου 2019). Παρουσίαση της Διδακτορικής Διατριβής της Δήμητρας Τσιακκούρη (9 Μαΐου 2019). Παρουσίαση της Διδακτορικής Διατριβής της Ελένης Ηροδότου (8 ...
Cliquez sur Jaime et rejoignez notre communauté sur Facebook. En récompense, vous obtiendrez immédiatement un bon de réduction dune valeur de 5€. ...
presenting, from the historic town of kawit, cavite: and coming to you (almost) live from california, U.S.A. - - - miong: uhm, looks nice. but what is it, mom? oh, yeah. a layout of me in a frame. and its from my tita bjay all the way from the philippines? wow, thanks, tita bjay! youre…
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C. Cali, M. Wawrzyniak, C. Becker, B. Maco, M. Cantoni, A. Jorstad, B. Nigro, F. Grillo, V. De Paola, P. Fua, G.W. Knott. PLoS ONE, 2018 ...
Grow houses are homes devoted to growing marijuana. Learn why grow houses may be in your neighborhood, especially if you live in Cali or Vancouver.
Se va servi alaturi de orez brun fiert - cartofi si fasole - se vor cali usor in putin ulei de masline mai multe tipuri de legume ceapa, morcovi, pastarnac si usturoicand totul se rumeneste se vor adauga si cartofii cubulete care se vor mai lasa la prajit cateva detoxifiere regim.
Rahmanzadeh R, Hüttmann G, Gerdes J, Scholzen T (June 2007). "Chromophore-assisted light inactivation of pKi-67 leads to ... Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a ...
... chromophore assisted light inactivation, and multi-photon light microscopy. In vivo imaging studies in live animals have been ... GFP emits a photon in the green region of the light spectrum when excited by the absorption of light. The chromophore consists ... Normally, this substance would not be able to absorb light, but with the attached biosensor, light can be absorbed and emitted ... GFP has been modified by changing the wavelength of light absorbed to include other colors of fluorescence. YFP or yellow ...
Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Lin JY, Sann SB, Zhou K, Nabavi ...
CO2 for 1-2 d after green light irradiation. Chromophore-assisted laser inactivation (CALI) was induced by applying green light ... Chromophore-assisted light inactivation of Centrin2. Mouse embryos were electroporated with Centrin2-KR/F-GFP at E15 and acute ... Light-induced inactivation of Centrin2 disrupts axon formation and neuronal migration in situ. A, Centrin2-KR fusion protein ( ... Disruption of the cytoplasmic polarity occurred within 10 min of Centrin2 inactivation, as evidenced by changes in the F-GFP ...
Chromophore-assisted light inactivation of proteins in vivo. Genetically targeted chromophore-assisted light inactivation. Tour ... chromophore-assisted light inactivation of proteins in vivo; protein design; nucleolar associations. Genome Biology volume 5, ... chromophore-assisted light inactivation of proteins in vivo; protein design; nucleolar associations. Genome Biol 5, 306 (2003 ... chromophore-assisted light inactivation of proteins in vivo, design of a novel protein fold and nucleolar clustering of tRNA ...
Chromophore-assisted Light Inactivation. A technique for causing a targeted loss of molecular function from REACTIVE OXYGEN ...
25, 2013 Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Lin et al.Neuron 2013 ... Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Lin et al.Neuron 2013 79(2):241 ...
Chromophore-assisted light inactivation of HaloTag fusion proteins labeled with eosin in living cells. ... Accidental amplification and inactivation of a methyltransferase gene eliminates cytosine methylation in Mycosphaerella ...
2013) Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI) Neuron, in press. ... Two light-triggered heterodimerization systems have been published (55⇓-57). Robust light activation of the flavin-based ... and red light may be replaced by CRY2, CIBN, and blue light, respectively. Nuclear localization signals may have to be provided ... such as light. The light would only need to propagate diffusely throughout the brain region of interest without requiring image ...
Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans.; ... Assisted maturation of native fistula in two patients with a continuous flow left ventricular assist device.; Journal of ... Light delivery over extended time periods enhances the effectiveness of photodynamic therapy.; Clinical cancer research : an ... Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium.; Free radical ...
Rahmanzadeh R, Hüttmann G, Gerdes J, Scholzen T (June 2007). "Chromophore-assisted light inactivation of pKi-67 leads to ... Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a ...
monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation Insert monomeric photosensitizing ...
Chromophore-assisted light inactivation and self-organization of microtubules and motors. Proc Natl Acad Sci U S A. 1998;95: ... These RNAs accumulate after infection with virus that has been inactivated by treatment with UV light, indicating that the ... our work suggests that experimental evolution can also shed light on the function and regulation of individual metabolic ... and may also shed light on a broad class of glycosyltransferases. .. ...
Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nat. Protoc. 2006;1(2):947- ... Jimenez-Banzo A, Nonell S, Hofkens J, Flors C. Singlet oxygen photosensitization by EGFP and its chromophore HBDI. Biophys. J. ... After white light illumination the transfected cell lines except the control cells stopped the proliferation. Measurements of ... Cell toxic effects caused by KRED after white light exposition are already documented in eukaryotes20 but data concerning the ...
2006). Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nat. Protoc. 1, 947- ... C with a photoperiod of 14 hours of light and 10 hours of darkness (Westerfield, 1995). Since temperature, light cycles and ... V.L.T. assisted in the design of the hormone treatments and provided critical reading of the manuscript. M.-A.A. conceived the ... Fish were maintained at 28.5°C with a photoperiod of 14 hours of light and 10 hours of darkness, and fed regularly (Westerfield ...
These ROS-generating proteins (RGPs) are widely used for cell ablation, mutagenesis and chromophore-assisted light inactivation ... We report that miniSOG produces both 1O2 and O2•-, as can its free chromophore, flavin mononucleotide. miniSOG produced O2•- at ... We measured the light-dependent O2•- production of purified miniSOG using HPLC separation of dihydroethidium (DHE) oxidation ... Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium.. Barnett ME1, ...
Genetically targeted chromophore-assisted light inactivation. Nat. Biotechnol. 21:1505-1508. doi:10.1038/nbt914. ... A general system for evaluating the efficiency of chromophore-assisted light inactivation (CALI) of proteins reveals Ru(II) ... Fluorophore-assisted light inactivation: a high-throughput tool for direct target validation of proteins. Proteomics. 2:247-255 ... Fluorophore-assisted light inactivation produces both targeted and collateral effects on N-type calcium channel modulation in ...
They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced ... Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nat Protoc 1 (2), 947-53 [+] ... Light sensitivity found in the DsRed neutral form, resulting in its instant transformation to the mature red chromophore, could ... KillerRed opens up new possibilities for precise light-induced cell killing and target protein inactivation. Because KillerRed ...
1998) Chromophore-assisted light inactivation and self-organization of microtubules and motors. Proceedings of the National ...
1998). Chromophore-assisted light inactivation and self-organization of microtubules and motors. Proc. Natl. Acad. Sci. USA 95 ... Chromophore-assisted light inactivation has a successful history and is based on validated physical theory (Surrey et al., 1998 ... 1999). Chromophore-assisted laser inactivation (CALI) to elucidate cellular mechanisms of cancer. Biochim. Biophys. Acta 1424, ... 1994). Chromophore-assisted laser inactivation of proteins is mediated by the photogeneration of free radicals. Proc. Natl. ...
"Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans." ... "Assisted maturation of native fistula in two patients with a continuous flow left ventricular assist device." Journal of ... Prizant H, Taya M, Lerman I, Light A, Sen A, Mitra S, Foster T, Hammes SR. "Estrogen maintains myometrial tumors in a ... "Light delivery over extended time periods enhances the effectiveness of photodynamic therapy." Clinical cancer research : an ...
Mice injected with the antibody combined with a light-sensitive molecule received green light irradiation at the hippocampus, ... A neurotransmitter receptor was inactivated at synapses of the mouse hippocampus using a light-induced method of protein ... modified a light-induced means of producing a burst of destructive oxygen (CALI: chromophore-assisted light inactivation) by ... It uses light irradiation to generate a temporary toxic form of oxygen that causes an area of damage shorter than a typical ...
He developed Chromophore Assisted Light Inactivation (CALI) of Protein Function and applied this technology functional ... He developed Chromophore Assisted Light Inactivation (CALI) of Protein Function and applied this technology functional ...
... engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are ... Chromophore-assisted laser inactivation (CALI) is a technique whereby ... Chromophore-assisted laser inactivation in cell biology Trends Cell Biol. 2008 Sep;18(9):443-50. doi: 10.1016/j.tcb.2008.07.001 ... Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce ...
R. Rahmanzadeh, G. Hüttmann, J. Gerdes, and T. Scholzen, "Chromophore-assisted light inactivation of pKi-67 leads to inhibition ... Examination of three tumor slides from each specimen was done with an Olympus CX31 light microscope. Photos were obtained by a ... examination of all prepared slides from each specimen was done with an Olympus CX31 light microscope. Photos were obtained from ... reflecting that miRNA-34a can work in a cell type-specific manner with a differential p53 pathway inactivation [36]. ...
... light has been reported as an effective agent for inactivation of specific proteins by chromophore-assisted light inactivation ... The phenomenon of FRET is not mediated by photon emission, and furthermore, does not even require the acceptor chromophore to ... The amount of time necessary for maturation of the chromophore ranges from several minutes to many hours, depending upon the ... Upon photobleaching mVenus in the cell shown in the lower portion of the window with 514-nanometer light (Figure 8(f)), ...
Rahmanzadeh R, Huttmann G, Gerdes J, Scholzen T. Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ... assisted by over-expression of fetal hemoglobins, protects from inflammatory colitis and reduces inflammatory cytokine ...
... chromophore assisted light inactivation, and multi-photon light microscopy. In vivo imaging studies in live animals have been ... GFP emits a photon in the green region of the light spectrum when excited by the absorption of light. The chromophore consists ... Normally, this substance would not be able to absorb light, but with the attached biosensor, light can be absorbed and emitted ... GFP has been modified by changing the wavelength of light absorbed to include other colors of fluorescence. YFP or yellow ...
Micro-scale chromophore-assisted laser inactivation of nerve growth cone proteins. Microsc. Res. Tech. 48:97-106. ... ML-7 inhibits the myosin light chain kinase through competitive binding of the ATP-binding site (Saitoh et al., 1987). ML-7 was ... Chromophore-assisted laser inactivation of cellular proteins. Methods Cell Biol. 44:715-732. ... Selective destruction of protein function by chromophore-assisted laser inactivation. Proc. Natl. Acad. Sci. USA. 85:5454-5458. ...
A general system for evaluating the efficiency of chromophore-assisted light inactivation (CALI) of proteins reveals Ru(ii) ...
Chromophore-Assisted Light Inactivation * Actins * Axons * NEONATAL PARENTERAL NUTRITION AND HEPATIC DYSFUNCTION. Bhatia, J. J. ...
To this end, we will try to inactivate cofilin via light, using chromophore-assisted light inactivation (CALI). So far we could ... Our findings likely reflect this effect and point to the idea that the inactivation of cofilin in some ways may lead to the ... To overcome these problems, the development of techniques that utilize a light-sensitive channel has been actively pursued. ...
  • Addgene: Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). (addgene.org)
  • Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. (nih.gov)
  • Work at Japanese institutions, including Yokohama City University, Osaka University and the University of Tokyo, modified a light-induced means of producing a burst of destructive oxygen (CALI: chromophore-assisted light inactivation) by incorporating an antibody to achieve specificity in protein inactivation. (eurekalert.org)
  • Here we address the role of myosin II (MII) in chick dorsal root ganglion neuronal growth cone motility and the contribution of M1c and MII to retrograde F-actin flow using chromophore-assisted laser inactivation (CALI). (rupress.org)
  • To this end, we will try to inactivate cofilin via light, using chromophore-assisted light inactivation (CALI). (ehime-u.ac.jp)
  • As for molecular targets, the chromophore-assisted light inactivation (CALI) will be used. (elsevier.com)
  • Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. (cellimagelibrary.org)
  • Finally, photoablation and chromophore-assisted laser inactivation (CALI) employ non-specific, non-reversible protein destruction using high power illumination. (grantome.com)
  • Chromophore-assisted laser inactivation (CALI) is a light-mediated technique that offers precise spatiotemporal control of protein inactivation, enabling better understanding of the protein's role in cell function. (grantome.com)
  • EGFP has been used effectively as a CALI chromophore, and its cotranslational attachment to the target protein avoids having to use exogenously added labeling reagents. (grantome.com)
  • A potential drawback to EGFP-CALI is that the CALI phenotype can be obscured by the endogenous, unlabeled protein that is not susceptible to light inactivation. (grantome.com)
  • Chromophore-assisted laser or light inactivation (CALI) has been employed as a promising technique to achieve spatiotemporal knockdown or loss-of-function of target molecules in situ . (biologists.org)
  • Chromophore-assisted laser or light inactivation (CALI) is a powerful technique for microscopic investigation of the functions of a protein of interest in situ and in a spatiotemporally regulated manner. (biologists.org)
  • The first report of CALI described the inactivation of alkaline phosphatase and β-galactosidase by using malachite-green-conjugated antibodies ( Jay, 1988 ). (biologists.org)
  • A selection of evaluations from Faculty of 1000 covering a Drosophila protein interaction map, profiling the mammalian AP-1 pathway, chromophore-assisted light inactivation of proteins in vivo , design of a novel protein fold and nucleolar clustering of tRNA genes. (biomedcentral.com)
  • Light-induced oxidant production by fluorescent proteins. (rochester.edu)
  • These ROS-generating proteins (RGPs) are widely used for cell ablation, mutagenesis and chromophore-assisted light inactivation of target proteins. (nih.gov)
  • Light-induced blockade of ER exit immediately perturbed Golgi membranes, and surprisingly, revealed that cis-Golgi-resident proteins continuously cycle to peripheral ER-Golgi intermediate compartment (ERGIC) membranes and depend on ER exit for their return to the Golgi. (biologists.org)
  • This project aims to build a novel tool to inactivate specific proteins in live cells with high spatial and temporal control by developing a light-activated site-specific protease in combination with a protease-sensitive version of a target protein of interest. (grantome.com)
  • Methods and constructs are provided for controlling processes in live animals, plants or microbes via genetically engineered near-infrared light-activated or light-inactivated proteins including chimeras including the photosensory modules of bacteriohytochromes and output modules that possess enzymatic activity and/or ability to bind to DMA, RNA, protein, or small molecules. (patentsencyclopedia.com)
  • DNA encoding these proteins are introduced as genes into live animals, plants or microbes, where their activities can be turned on by near-infrared light, controlled by the intensity of light, and turned off by near-infrared light of a different wavelength than the activating light. (patentsencyclopedia.com)
  • These proteins can regulate diverse cellular processes with high spatial and temporal precision, in a nontoxic manner, often using external light sources. (patentsencyclopedia.com)
  • For example, near-Infrared light-activated proteins possessing nucleotidyl cyclase, protein kinase, protease, DNA-binding and RNA-binding activities are useful to control signal transduction, cell apopiosis, proliferation, adhesion, differentiation and other cell processes. (patentsencyclopedia.com)
  • wherein the level of said desired activity of said fusion proteins is controllable by NIR light when the level of said desired activity is changed by the presence and/or absence of NIR light having said selected wavelengths. (patentsencyclopedia.com)
  • 2. The method of claim 1 for producing fusion proteins with enhanced controllability by NIR light, wherein such enhanced controllability exists when said fusion proteins have high ratios of activity in the light versus dark or vice versa. (patentsencyclopedia.com)
  • transforming suitable organisms, with this DNA, expressing the DNA, and screening the resultant fusion proteins for additional fusion proteins controllable by NIR light. (patentsencyclopedia.com)
  • Photosensitizing fluorescent proteins, which generate reactive oxygen species (ROS) upon light irradiation, are useful for spatiotemporal protein inactivation and cell ablation. (beds.ac.uk)
  • Through absorption of the laser light, chromophores become excited, react with oxygen and generate reactive oxygen species (ROS) that, in turn, cause damage to DNA, RNA, lipids and proteins. (biologists.org)
  • 4. The method of claim 3 wherein said test organisms do not comprise an endogenous chromophore. (patentsencyclopedia.com)
  • Natural organic matter (NOM) has a large influence, as it can attenuate radiation and thus decrease inactivation by endogenous mechanisms. (rsc.org)
  • Genetically targeted chromophore-assisted light inactivation. (biomedcentral.com)
  • Genetically-encoded photosensitizers produce reactive oxygen species (ROS) in response to light. (nih.gov)
  • The objective of this project is to address this challenge by developing an innovative, versatile, genetically-encoded method by which a protein of interest can be disrupted by specific light-activated proteolysis in either whole cells or intracellular regions as a novel tool to analyze protein function in live cells. (grantome.com)
  • Since ROS generation of a photosensitizer is specifically controlled by certain excitation wavelengths, utilizing colour variants of photosensitizing protein would allow multi-spatiotemporal control of inactivation. (beds.ac.uk)
  • As a proof of concept for multi-spatiotemporal control of inactivation, we demonstrate that SuperNova Green can be used with its red variant, SuperNova, to perform independent protein inactivation or cell ablation studies in a spatiotemporal manner by selective light irradiation. (beds.ac.uk)
  • So, by using a focused laser beam and controlling the generation of ROS within a specific subcellular area with micrometer to sub-micrometer accuracy, the spatiotemporal inactivation of targets can be achieved. (biologists.org)
  • min/µmol photosensitizer for an excitation fluence rate of 5.9mW/mm 2 at 470 ± 20nm, and the rate remained consistent across fluences (light doses). (nih.gov)
  • In the present work, researchers made an antibody against the outer part of the neurotransmitter receptor GluA1 that they labeled with a light-sensitive molecule (a photosensitizer). (eurekalert.org)
  • PURPOSE: Near-infrared photoimmunotherapy (NIR-PIT) is a localized molecular cancer therapy combining a photosensitizer-conjugated monoclonal antibody and light energy. (stanford.edu)
  • It uses light irradiation to generate a temporary toxic form of oxygen that causes an area of damage shorter than a typical protein-protein interaction distance. (eurekalert.org)
  • In agreement with these reactivities, this compound exhibited an exciting antibacterial action, particularly against Gram-positive bacteria Staphylococcus aureus and Staphylococcus epidermidis, which was enhanced upon blue light irradiation. (bvsalud.org)
  • SuperNova Green is able to produce ROS spatiotemporally upon blue light irradiation. (beds.ac.uk)
  • This showed that the fear memory had been erased by the inactivation of synaptic GluA1. (eurekalert.org)
  • Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium. (rochester.edu)
  • Methods pertaining to the generation of new individuals, including techniques used in selective BREEDING, cloning (CLONING, ORGANISM), and assisted reproduction (REPRODUCTIVE TECHNIQUES, ASSISTED). (umassmed.edu)
  • In this critical review, we summarize the photo-physics, -chemistry, and -biology that underpin sunlight-mediated inactivation, as well as the targets of damage and cellular responses to sunlight exposure. (rsc.org)
  • In mammalian systems, few approaches allow acute inactivation and as a consequence the immediate effects of a blockade of membrane trafficking are largely unknown. (biologists.org)
  • Therefore, we sought a technique for rapid protein inactivation while carrying out live-cell imaging experiments to better understand Golgi dynamics. (biologists.org)
  • Here, we describe the synthesis, characterization, and utility of two photosensitizers based upon the widely used rhodamine scaffold and demonstrate their efficacy for chromophore-assisted light inactivation, cell ablation in culture and in vivo, and photopolymerization of diaminobenzidine for electron microscopy. (janelia.org)
  • After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, KDR cells exhibited dynamic changes on the dorsal surface of the cell, forming numerous protrusions as seen in this DIC time-lapse movie. (cellimagelibrary.org)
  • Light-induced blockage of cell division with a chromatin-targeted phototoxic fluorescent protein. (evrogen.ru)
  • Inactivation of antigen MKI67 leads to inhibition of ribosomal RNA synthesis.The MKI67 protein is a nuclear and nucleolar protein, which is tightly associated with somatic cell proliferation. (sinobiological.com)
  • Development of SuperNova Green has expanded the photosensitizing protein toolbox to optogenetically control protein inactivation and cell ablation. (beds.ac.uk)
  • 3. The apparatus of claim 1 or 2 , whereby the focal volume comprises electromagnetic radiation sufficient to affect the cell of interest resulting in a cellular response selected from the group of cell death, transient permeabilization of the cell membrane, activation of a photosensitive agent, or inactivation of a cell component. (google.com)
  • Cholesterol-Assisted Bacterial Cell Surface Engineering for Photodynamic Inactivation of Gram-Positive and Gram-Negative Bacteria. (semanticscholar.org)
  • Viruses that are not susceptible to exogenous inactivation are only inactivated if UVB wavelengths (280-320 nm) are present, such as in very clear, open waters or in containers that are transparent to UVB. (rsc.org)
  • Some viruses and bacteria (especially Gram-positive) are susceptible to exogenous inactivation, which can be initiated by visible as well as UV wavelengths. (rsc.org)
  • In response to illumination of the mouse hippocampus with green light, we found that mice returned to the dark boxes more quickly than control animals," study first author Kiwamu Takemoto says. (eurekalert.org)
  • Because we propose to use light to toggle protease activity, experiments can be carried out entirely on an adequately equipped microscope allowing unprecedented high temporal and spatial control of intracellular protein inactivation by using patterned illumination. (grantome.com)
  • To test and expand upon this concept, we monitored autofluorescence and light scattering intensities of injured vs. uninjured rat kidneys via multimodal imaging under 355 nm, 325 nm, and 266 nm excitation as well as scattering under 500 nm illumination. (spie.org)
  • Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. (wikipedia.org)
  • 2007) Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. (sinobiological.com)
  • The implications of this mechanistic understanding of solar inactivation are discussed for a range of applications, including recreational water quality, natural treatment systems, solar disinfection of drinking water (SODIS), and enhanced inactivation via the use of sensitizers and photocatalysts. (rsc.org)
  • SummaryDespite of the approval of Photofrin® in various countries, chemically defined sensitizers for photodynamic therapy (PDT) are still needed for the absorption of light in the infrared spectrum, which provides a maximal penetration of light into tissue. (semanticscholar.org)
  • Ruthenium polypyridine complexes have shown promise as agents for photodynamic therapy (PDT) and tools for molecular biology (chromophore-assisted light inactivation). (bvsalud.org)
  • 3. The method of claim 1 also comprising transforming said test organisms with DNA encoding a heme oxygenase gene capable of being expressed in said test organisms to produce a biliverdin IXquadrature chromophore. (patentsencyclopedia.com)
  • We review approaches to model sunlight-mediated inactivation and illustrate how the environmental conditions can dramatically shift the inactivation rate of organisms. (rsc.org)
  • 2 Sunlight is the key factor contributing to inactivation of indicator organisms and pathogens in engineered natural systems like wastewater treatment ponds (WTP) 3 and open-water wetlands for treatment of wastewater and stormwater. (rsc.org)
  • In this project we not only plan to experimentally demonstrate the crucial role of the phonon-assisted dynamics in facilitating efficient energy transfer in chromophoric complexes and odour recognition, but also to develop a general theoretical framework to describe and understand the role coherent vibrations play in the dynamics of biomolecular systems, as well as to develop methods to identify the presence and properties of such vibrations. (thefreedictionary.com)
  • Such FPs can absorb visible day-light and predominantly one variant of the red fluorescent protein, the KillerRed protein (KRED) emits active electrons producing reactive oxygen species (ROS) leading to photokilling processes in eukaryotes. (medsci.org)
  • To accurately predict inactivation and design engineered systems that enhance solar inactivation, it is necessary to model these processes, although some details are not yet sufficiently well understood. (rsc.org)
  • Typical fluorescence microscopy techniques rely upon the absorption of light by a fluorophore at one wavelength (excitation), followed by the subsequent emission of secondary fluorescence at a longer wavelength. (zeiss.com)
  • Photosensitizers, compounds that generate reactive oxygen species upon excitation with light, can be utilized for numerous biological experiments, but the repertoire of bioavailable photosensitizers is limited. (janelia.org)
  • Different isomeric manifestations of the molecule absorbs different wavelengths of light, so that each isomeric species can display a different color based on its absorption. (wikipedia.org)
  • Indwelling Stent Embedded with Light-Emitting Diodes for Photodynamic Therapy of Malignant Biliary Obstruction. (rochester.edu)
  • Together, these new tools facilitate light-microscopy-based neuronal tracing at both a large scale and a high resolution. (elifesciences.org)
  • Automatic and adaptive heterogeneous refractive index compensation for light-sheet microscopy. (ucdenver.edu)
  • In SAX microscopy, 10 improvement in spatial resolution is achieved by exploiting the nonlinearity in the fluorescence signals, which is induced when the target fluorescence molecule excited states become saturated due to the excited-state lifetime and the limited number of fluorescence molecules in the focal volume of the laser light. (spiedigitallibrary.org)
  • However, this is only informative if the inactivation is precise with respect to space and time. (eurekalert.org)
  • Photosensitizers are chromophores which generate excessive reactive oxygen species (ROS) when irradiated by excitation light. (beds.ac.uk)
  • Fluorescence spectra can be detected from dye molecules, and native molecules in tissues samples with excitation from wavelengths extracted from ultrafast SC light in the spectral range between 350 to 500 nm using narrow bandpass filters. (spie.org)
  • For instance, in one embodiment, the reader can utilize a silicon photodiode and a pulsed light-emitting diode (LED) to accurately excite labels and detect fluorescence on a membrane-based assay device without requiring the use of expensive components, such as monochromators or narrow emission band width optical filters. (google.com.au)
  • Normally, this substance would not be able to absorb light, but with the attached biosensor, light can be absorbed and emitted on a spectrophotometer. (wikipedia.org)
  • Their ability to display different colors lies in how they absorb light. (wikipedia.org)
  • Any of various chemical groups that absorb light of certain wavelengths and when present in an organic compound, such as a dye or pigment, are responsible for the color of the compound. (thefreedictionary.com)
  • The segment polarity genes that have been most widely studied in other insects are engrailed ( en ) and wingless ( wg ). (biologists.org)
  • Laser light was focused onto the specimen plane through a 488-nm laser filter set (Z488/10, HQ525/50, Z488RDC, Chroma Technology Corporation). (cellimagelibrary.org)
  • Indocyanine green and laser light for the treatment of AIDS-associated cutaneous Kaposi's sarcoma. (semanticscholar.org)
  • The use of light interaction (scattering, absorption, and fluorescence) with biological tissue to obtain morphologically based information. (ucdenver.edu)
  • Interestingly, they also showed DNA photocleavage activity even upon exposure to a yellow light-emitting diode (LED). (bvsalud.org)
  • Method of making images on a sensitized surface by exposure to light or other radiant energy. (childrensmercy.org)
  • To overcome these problems, the development of techniques that utilize a light-sensitive channel has been actively pursued. (ehime-u.ac.jp)
  • Afin de comprendre comment les émotions sont encodées et transmises dans ces structures, et comment elles affectent le comportement, l'équipe du Pr Proulx utilise des techniques avancées (photométrie par fibre optique, mesures d'électrophysiologie, optogénétique) qui lui permettent de mesurer et de manipuler l'activité neuronale, en temps réel chez des animaux libres de mouvement. (ulaval.ca)
  • This complex was subjected to blue and green light photolysis (453 and 505 nm, respectively) in aqueous solution, leading to the release of methionine and formation of the cis-[Ru(bpy)2(H2O)2]2+ ion. (bvsalud.org)
  • Inactivation is accomplished using a device having a unique temperature control design. (google.ca)
  • Light-mediated chemical reactions are powerful methods for manipulating and interrogating biological systems. (janelia.org)