Chromophore-Assisted Light Inactivation
Ultraviolet Rays
That portion of the electromagnetic spectrum immediately below the visible range and extending into the x-ray frequencies. The longer wavelengths (near-UV or biotic or vital rays) are necessary for the endogenous synthesis of vitamin D and are also called antirachitic rays; the shorter, ionizing wavelengths (far-UV or abiotic or extravital rays) are viricidal, bactericidal, mutagenic, and carcinogenic and are used as disinfectants.
Retinaldehyde
A carotenoid constituent of visual pigments. It is the oxidized form of retinol which functions as the active component of the visual cycle. It is bound to the protein opsin forming the complex rhodopsin. When stimulated by visible light, the retinal component of the rhodopsin complex undergoes isomerization at the 11-position of the double bond to the cis-form; this is reversed in "dark" reactions to return to the native trans-configuration.
Tubulin Modulators
Nocodazole
Microtubules
Neurons
Golgi Apparatus
A stack of flattened vesicles that functions in posttranslational processing and sorting of proteins, receiving them from the rough ENDOPLASMIC RETICULUM and directing them to secretory vesicles, LYSOSOMES, or the CELL MEMBRANE. The movement of proteins takes place by transfer vesicles that bud off from the rough endoplasmic reticulum or Golgi apparatus and fuse with the Golgi, lysosomes or cell membrane. (From Glick, Glossary of Biochemistry and Molecular Biology, 1990)
Stathmin
A ubiquitous phosphoprotein that serves as an intracellular substrate for a variety of SIGNAL TRANSDUCTION PATHWAYS. PHOSPHORYLATION of stathmin occurs during CELL CYCLE progression, and stathmin functions as a microtubule-destabilizing protein that promotes MICROTUBULE depolymerization during INTERPHASE and late MITOSIS. Stathmin is expressed at very high levels in a variety of human CANCERS.
Colchicine
Optogenetics
Quebec
A province of eastern Canada. Its capital is Quebec. The region belonged to France from 1627 to 1763 when it was lost to the British. The name is from the Algonquian quilibek meaning the place where waters narrow, referring to the gradually narrowing channel of the St. Lawrence or to the narrows of the river at Cape Diamond. (From Webster's New Geographical Dictionary, 1988, p993 & Room, Brewer's Dictionary of Names, 1992, p440)
Rosaniline Dyes
Ubiquitin-Protein Ligases
A diverse class of enzymes that interact with UBIQUITIN-CONJUGATING ENZYMES and ubiquitination-specific protein substrates. Each member of this enzyme group has its own distinct specificity for a substrate and ubiquitin-conjugating enzyme. Ubiquitin-protein ligases exist as both monomeric proteins multiprotein complexes.
PubMed
A bibliographic database that includes MEDLINE as its primary subset. It is produced by the National Center for Biotechnology Information (NCBI), part of the NATIONAL LIBRARY OF MEDICINE. PubMed, which is searchable through NLM's Web site, also includes access to additional citations to selected life sciences journals not in MEDLINE, and links to other resources such as the full-text of articles at participating publishers' Web sites, NCBI's molecular biology databases, and PubMed Central.
Wood
Publishing
MEDLINE
Serial Publications
Diffusion
Oxygen
Blood Flow Velocity
Xenon Radioisotopes
Cisterna Magna
Brefeldin A
Endoplasmic Reticulum
A system of cisternae in the CYTOPLASM of many cells. In places the endoplasmic reticulum is continuous with the plasma membrane (CELL MEMBRANE) or outer membrane of the nuclear envelope. If the outer surfaces of the endoplasmic reticulum membranes are coated with ribosomes, the endoplasmic reticulum is said to be rough-surfaced (ENDOPLASMIC RETICULUM, ROUGH); otherwise it is said to be smooth-surfaced (ENDOPLASMIC RETICULUM, SMOOTH). (King & Stansfield, A Dictionary of Genetics, 4th ed)
Coatomer Protein
A 700-kDa cytosolic protein complex consisting of seven equimolar subunits (alpha, beta, beta', gamma, delta, epsilon and zeta). COATOMER PROTEIN and ADP-RIBOSYLATION FACTOR 1 are principle components of COAT PROTEIN COMPLEX I and are involved in vesicle transport between the ENDOPLASMIC RETICULUM and the GOLGI APPARATUS.
Photochemotherapy
Photosensitizing Agents
Drugs that are pharmacologically inactive but when exposed to ultraviolet radiation or sunlight are converted to their active metabolite to produce a beneficial reaction affecting the diseased tissue. These compounds can be administered topically or systemically and have been used therapeutically to treat psoriasis and various types of neoplasms.
Publications
Dihematoporphyrin Ether
The purified component of HEMATOPORPHYRIN DERIVATIVE, it consists of a mixture of oligomeric porphyrins. It is used in photodynamic therapy (HEMATOPORPHYRIN PHOTORADIATION); to treat malignant lesions with visible light and experimentally as an antiviral agent. It is the first drug to be approved in the use of PHOTODYNAMIC THERAPY in the United States.
Hepatolenticular Degeneration
A rare autosomal recessive disease characterized by the deposition of copper in the BRAIN; LIVER; CORNEA; and other organs. It is caused by defects in the ATP7B gene encoding copper-transporting ATPase 2 (EC 3.6.3.4), also known as the Wilson disease protein. The overload of copper inevitably leads to progressive liver and neurological dysfunction such as LIVER CIRRHOSIS; TREMOR; ATAXIA and intellectual deterioration. Hepatic dysfunction may precede neurologic dysfunction by several years.
Academic Medical Centers
Fear
Memory
Hippocampus
A curved elevation of GRAY MATTER extending the entire length of the floor of the TEMPORAL HORN of the LATERAL VENTRICLE (see also TEMPORAL LOBE). The hippocampus proper, subiculum, and DENTATE GYRUS constitute the hippocampal formation. Sometimes authors include the ENTORHINAL CORTEX in the hippocampal formation.
RNA, Small Interfering
Small double-stranded, non-protein coding RNAs (21-31 nucleotides) involved in GENE SILENCING functions, especially RNA INTERFERENCE (RNAi). Endogenously, siRNAs are generated from dsRNAs (RNA, DOUBLE-STRANDED) by the same ribonuclease, Dicer, that generates miRNAs (MICRORNAS). The perfect match of the siRNAs' antisense strand to their target RNAs mediates RNAi by siRNA-guided RNA cleavage. siRNAs fall into different classes including trans-acting siRNA (tasiRNA), repeat-associated RNA (rasiRNA), small-scan RNA (scnRNA), and Piwi protein-interacting RNA (piRNA) and have different specific gene silencing functions.
Amygdala
Selective fluorophore-assisted light inactivation of voltage-gated calcium channels. (1/5)
(+info)A trifluoroacetic acid-labile sulfonate protecting group and its use in the synthesis of a near-IR fluorophore. (2/5)
(+info)Bringing optogenetics to the synapse. (3/5)
(+info)Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). (4/5)
(+info)SuperNova, a monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation. (5/5)
(+info)
Journal Club Archive | Research in Neuroscience - McGill University
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Ki-67 (protein)
Rahmanzadeh R, Hüttmann G, Gerdes J, Scholzen T (June 2007). "Chromophore-assisted light inactivation of pKi-67 leads to ... Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a ...
Fluorescent tag
... chromophore assisted light inactivation, and multi-photon light microscopy. In vivo imaging studies in live animals have been ... GFP emits a photon in the green region of the light spectrum when excited by the absorption of light. The chromophore consists ... Normally, this substance would not be able to absorb light, but with the attached biosensor, light can be absorbed and emitted ... GFP has been modified by changing the wavelength of light absorbed to include other colors of fluorescence. YFP or yellow ...
Addgene: Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI).
Plus it
CO2 for 1-2 d after green light irradiation. Chromophore-assisted laser inactivation (CALI) was induced by applying green light ... Chromophore-assisted light inactivation of Centrin2. Mouse embryos were electroporated with Centrin2-KR/F-GFP at E15 and acute ... Light-induced inactivation of Centrin2 disrupts axon formation and neuronal migration in situ. A, Centrin2-KR fusion protein ( ... Disruption of the cytoplasmic polarity occurred within 10 min of Centrin2 inactivation, as evidenced by changes in the F-GFP ...
Articles selected by Faculty of 1000 : Drosophila protein interaction map; profiling the mammalian AP-1 pathway; chromophore...
Chromophore-assisted light inactivation of proteins in vivo. Genetically targeted chromophore-assisted light inactivation. Tour ... chromophore-assisted light inactivation of proteins in vivo; protein design; nucleolar associations. Genome Biology volume 5, ... chromophore-assisted light inactivation of proteins in vivo; protein design; nucleolar associations. Genome Biol 5, 306 (2003 ... chromophore-assisted light inactivation of proteins in vivo, design of a novel protein fold and nucleolar clustering of tRNA ...
Individually addressable multi-nanopores for single-molecule targeted operations.
Journal Club Archive | Research in Neuroscience - McGill University
Ki-67 (protein) - Wikipedia
Wood KV[au] - PubMed - NCBI
Very long-term memories may be stored in the pattern of holes in the perineuronal net | PNAS
2013) Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI) Neuron, in press. ... Two light-triggered heterodimerization systems have been published (55⇓-57). Robust light activation of the flavin-based ... and red light may be replaced by CRY2, CIBN, and blue light, respectively. Nuclear localization signals may have to be provided ... such as light. The light would only need to propagate diffusely throughout the brain region of interest without requiring image ...
Publications - Foster Lab - University of Rochester Medical Center
Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans.; ... Assisted maturation of native fistula in two patients with a continuous flow left ventricular assist device.; Journal of ... Light delivery over extended time periods enhances the effectiveness of photodynamic therapy.; Clinical cancer research : an ... Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium.; Free radical ...
Addgene: Search results
Experts and Doctors on molecular sequence data in Princeton, Florida, United States
Chromophore-assisted light inactivation and self-organization of microtubules and motors. Proc Natl Acad Sci U S A. 1998;95: ... These RNAs accumulate after infection with virus that has been inactivated by treatment with UV light, indicating that the ... our work suggests that experimental evolution can also shed light on the function and regulation of individual metabolic ... and may also shed light on a broad class of glycosyltransferases. .. ...
Autofluorescent Proteins as Photosensitizer in Eukaryontes
Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nat. Protoc. 2006;1(2):947- ... Jimenez-Banzo A, Nonell S, Hofkens J, Flors C. Singlet oxygen photosensitization by EGFP and its chromophore HBDI. Biophys. J. ... After white light illumination the transfected cell lines except the control cells stopped the proliferation. Measurements of ... Cell toxic effects caused by KRED after white light exposition are already documented in eukaryotes20 but data concerning the ...
Regeneration of breeding tubercles on zebrafish pectoral fins requires androgens and two waves of revascularization |...
2006). Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nat. Protoc. 1, 947- ... C with a photoperiod of 14 hours of light and 10 hours of darkness (Westerfield, 1995). Since temperature, light cycles and ... V.L.T. assisted in the design of the hormone treatments and provided critical reading of the manuscript. M.-A.A. conceived the ... Fish were maintained at 28.5°C with a photoperiod of 14 hours of light and 10 hours of darkness, and fed regularly (Westerfield ...
Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium. - PubMed - NCBI
These ROS-generating proteins (RGPs) are widely used for cell ablation, mutagenesis and chromophore-assisted light inactivation ... We report that miniSOG produces both 1O2 and O2•-, as can its free chromophore, flavin mononucleotide. miniSOG produced O2•- at ... We measured the light-dependent O2•- production of purified miniSOG using HPLC separation of dihydroethidium (DHE) oxidation ... Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium.. Barnett ME1, ...
Antibody-guided photoablation of voltage-gated potassium currents | JGP
Genetically targeted chromophore-assisted light inactivation. Nat. Biotechnol. 21:1505-1508. doi:10.1038/nbt914. ... A general system for evaluating the efficiency of chromophore-assisted light inactivation (CALI) of proteins reveals Ru(II) ... Fluorophore-assisted light inactivation: a high-throughput tool for direct target validation of proteins. Proteomics. 2:247-255 ... Fluorophore-assisted light inactivation produces both targeted and collateral effects on N-type calcium channel modulation in ...
Лукьянов Сергей Анатольевич - ИБХ РАН
They are used for inactivation of specific proteins by chromophore-assisted light inactivation (CALI) and for light-induced ... Chromophore-assisted light inactivation (CALI) using the phototoxic fluorescent protein KillerRed. Nat Protoc 1 (2), 947-53 [+] ... Light sensitivity found in the DsRed neutral form, resulting in its instant transformation to the mature red chromophore, could ... KillerRed opens up new possibilities for precise light-induced cell killing and target protein inactivation. Because KillerRed ...
Items where Person is "Nédélec-F" - CaltechAUTHORS
Irradiation-induced protein inactivation reveals Golgi enzyme cycling to cell periphery | Journal of Cell Science
1998). Chromophore-assisted light inactivation and self-organization of microtubules and motors. Proc. Natl. Acad. Sci. USA 95 ... Chromophore-assisted light inactivation has a successful history and is based on validated physical theory (Surrey et al., 1998 ... 1999). Chromophore-assisted laser inactivation (CALI) to elucidate cellular mechanisms of cancer. Biochim. Biophys. Acta 1424, ... 1994). Chromophore-assisted laser inactivation of proteins is mediated by the photogeneration of free radicals. Proc. Natl. ...
Publications by Thomas H. Foster, Ph.D. - University of Rochester Medical Center
"Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans." ... "Assisted maturation of native fistula in two patients with a continuous flow left ventricular assist device." Journal of ... Prizant H, Taya M, Lerman I, Light A, Sen A, Mitra S, Foster T, Hammes SR. "Estrogen maintains myometrial tumors in a ... "Light delivery over extended time periods enhances the effectiveness of photodynamic therapy." Clinical cancer research : an ...
Fluorescent tag - Wikipedia
... chromophore assisted light inactivation, and multi-photon light microscopy. In vivo imaging studies in live animals have been ... GFP emits a photon in the green region of the light spectrum when excited by the absorption of light. The chromophore consists ... Normally, this substance would not be able to absorb light, but with the attached biosensor, light can be absorbed and emitted ... GFP has been modified by changing the wavelength of light absorbed to include other colors of fluorescence. YFP or yellow ...
Chromophore-assisted laser inactivation in cell biology
... engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are ... Chromophore-assisted laser inactivation (CALI) is a technique whereby ... Chromophore-assisted laser inactivation in cell biology Trends Cell Biol. 2008 Sep;18(9):443-50. doi: 10.1016/j.tcb.2008.07.001 ... Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce ...
Precise inactivation of neural messenger receptor wipes out fear memory in mice | EurekAlert! Science News
Mice injected with the antibody combined with a light-sensitive molecule received green light irradiation at the hippocampus, ... A neurotransmitter receptor was inactivated at synapses of the mouse hippocampus using a light-induced method of protein ... modified a light-induced means of producing a burst of destructive oxygen (CALI: chromophore-assisted light inactivation) by ... It uses light irradiation to generate a temporary toxic form of oxygen that causes an area of damage shorter than a typical ...
Editorial Board ::: Current Proteomics
MicroRNA-34a: A Key Regulator in the Hallmarks of Renal Cell Carcinoma
R. Rahmanzadeh, G. Hüttmann, J. Gerdes, and T. Scholzen, "Chromophore-assisted light inactivation of pKi-67 leads to inhibition ... Examination of three tumor slides from each specimen was done with an Olympus CX31 light microscope. Photos were obtained by a ... examination of all prepared slides from each specimen was done with an Olympus CX31 light microscope. Photos were obtained from ... reflecting that miRNA-34a can work in a cell type-specific manner with a differential p53 pathway inactivation [36]. ...
Mixing Spectral Imaging and FRET Microscopy
... light has been reported as an effective agent for inactivation of specific proteins by chromophore-assisted light inactivation ... The phenomenon of FRET is not mediated by photon emission, and furthermore, does not even require the acceptor chromophore to ... The amount of time necessary for maturation of the chromophore ranges from several minutes to many hours, depending upon the ... Upon photobleaching mVenus in the cell shown in the lower portion of the window with 514-nanometer light (Figure 8(f)), ...
Wingless Enforces Strict Segregation | Science Signaling
Myosin 1c and myosin IIB serve opposing roles in lamellipodial dynamics of the neuronal growth cone | JCB
Micro-scale chromophore-assisted laser inactivation of nerve growth cone proteins. Microsc. Res. Tech. 48:97-106. ... ML-7 inhibits the myosin light chain kinase through competitive binding of the ATP-binding site (Saitoh et al., 1987). ML-7 was ... Chromophore-assisted laser inactivation of cellular proteins. Methods Cell Biol. 44:715-732. ... Selective destruction of protein function by chromophore-assisted laser inactivation. Proc. Natl. Acad. Sci. USA. 85:5454-5458. ...
Xiangshu Xiao - Publications
- Oregon Health & Science University
DeCS
Chromophore-Assisted Light Inactivation Entry term(s). Chromophore Assisted Light Inactivation Chromophore-Assisted Light ... Inactivation, Chromophore-Assisted Light Inactivations, Chromophore-Assisted Light Light Inactivation, Chromophore-Assisted ... Chromophore Assisted Laser Inactivation. Chromophore Assisted Light Inactivation. Chromophore-Assisted Laser Inactivation. ... Inactivation, Chromophore-Assisted Laser. Inactivation, Chromophore-Assisted Light. Inactivation, Fluorophore-Assisted Light. ...
CALIProteinsEndogenousGeneticallySpatiotemporalIrradiationPhotosensitizerPhotodynamic TherapyTechniqueSynapticPhotocatalystsSelectiveWavelengths of lightTargetsCellMammalianSusceptibleIlluminationInhibitionOrganismsDemonstrateProcessesAbsorption of liExperimentsIntensePreciseExcitationTechniquesAccuratelyMicroscopyAbsorb lightEffectivelyLaser lightWidelyControlGreenExposureOptical
CALI15
- Addgene: Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). (addgene.org)
- Chromophore-assisted laser inactivation (CALI) is a technique whereby engineered proteins and dye molecules that produce substantial amounts of reactive oxygen species upon absorption of light are used to perturb biological systems in a spatially and temporally defined manner. (nih.gov)
- Work at Japanese institutions, including Yokohama City University, Osaka University and the University of Tokyo, modified a light-induced means of producing a burst of destructive oxygen (CALI: chromophore-assisted light inactivation) by incorporating an antibody to achieve specificity in protein inactivation. (eurekalert.org)
- Here we address the role of myosin II (MII) in chick dorsal root ganglion neuronal growth cone motility and the contribution of M1c and MII to retrograde F-actin flow using chromophore-assisted laser inactivation (CALI). (rupress.org)
- To this end, we will try to inactivate cofilin via light, using chromophore-assisted light inactivation (CALI). (ehime-u.ac.jp)
- As for molecular targets, the chromophore-assisted light inactivation (CALI) will be used. (elsevier.com)
- Chromophore-assisted laser inactivation (CALI) inactivates target proteins by generating reactive photoproducts such as reactive oxygen species (ROS) by intense irradiation of photosensitizer chromophores, such as EGFP. (cellimagelibrary.org)
- Ligand-directed Ru(bpy) 3 photocatalysts induce chromophore-assisted light inactivation (CALI) of target proteins under visible light irradiation in vitro and within cells. (elsevier.com)
- Finally, photoablation and chromophore-assisted laser inactivation (CALI) employ non-specific, non-reversible protein destruction using high power illumination. (grantome.com)
- Chromophore-assisted laser inactivation (CALI) is a light-mediated technique that offers precise spatiotemporal control of protein inactivation, enabling better understanding of the protein's role in cell function. (grantome.com)
- EGFP has been used effectively as a CALI chromophore, and its cotranslational attachment to the target protein avoids having to use exogenously added labeling reagents. (grantome.com)
- A potential drawback to EGFP-CALI is that the CALI phenotype can be obscured by the endogenous, unlabeled protein that is not susceptible to light inactivation. (grantome.com)
- Chromophore-assisted laser or light inactivation (CALI) has been employed as a promising technique to achieve spatiotemporal knockdown or loss-of-function of target molecules in situ . (biologists.org)
- Chromophore-assisted laser or light inactivation (CALI) is a powerful technique for microscopic investigation of the functions of a protein of interest in situ and in a spatiotemporally regulated manner. (biologists.org)
- The first report of CALI described the inactivation of alkaline phosphatase and β-galactosidase by using malachite-green-conjugated antibodies ( Jay, 1988 ). (biologists.org)
Proteins16
- A selection of evaluations from Faculty of 1000 covering a Drosophila protein interaction map, profiling the mammalian AP-1 pathway, chromophore-assisted light inactivation of proteins in vivo , design of a novel protein fold and nucleolar clustering of tRNA genes. (biomedcentral.com)
- Light-induced oxidant production by fluorescent proteins. (rochester.edu)
- These ROS-generating proteins (RGPs) are widely used for cell ablation, mutagenesis and chromophore-assisted light inactivation of target proteins. (nih.gov)
- Light-induced blockade of ER exit immediately perturbed Golgi membranes, and surprisingly, revealed that cis-Golgi-resident proteins continuously cycle to peripheral ER-Golgi intermediate compartment (ERGIC) membranes and depend on ER exit for their return to the Golgi. (biologists.org)
- Other ion channels have been developed that are able to open and close much faster, mimicking the higher firing rate of some neurons, and a new wave of proteins modify protein-protein interactions in response to light, allowing us to control different cell types in a whole new way. (neuwritesd.org)
- Crystal Structure of Phototoxic Orange Fluorescent Proteins with a Tryptophan-Based Chromophore. (evrogen.ru)
- This project aims to build a novel tool to inactivate specific proteins in live cells with high spatial and temporal control by developing a light-activated site-specific protease in combination with a protease-sensitive version of a target protein of interest. (grantome.com)
- Methods and constructs are provided for controlling processes in live animals, plants or microbes via genetically engineered near-infrared light-activated or light-inactivated proteins including chimeras including the photosensory modules of bacteriohytochromes and output modules that possess enzymatic activity and/or ability to bind to DMA, RNA, protein, or small molecules. (patentsencyclopedia.com)
- DNA encoding these proteins are introduced as genes into live animals, plants or microbes, where their activities can be turned on by near-infrared light, controlled by the intensity of light, and turned off by near-infrared light of a different wavelength than the activating light. (patentsencyclopedia.com)
- These proteins can regulate diverse cellular processes with high spatial and temporal precision, in a nontoxic manner, often using external light sources. (patentsencyclopedia.com)
- For example, near-Infrared light-activated proteins possessing nucleotidyl cyclase, protein kinase, protease, DNA-binding and RNA-binding activities are useful to control signal transduction, cell apopiosis, proliferation, adhesion, differentiation and other cell processes. (patentsencyclopedia.com)
- wherein the level of said desired activity of said fusion proteins is controllable by NIR light when the level of said desired activity is changed by the presence and/or absence of NIR light having said selected wavelengths. (patentsencyclopedia.com)
- 2. The method of claim 1 for producing fusion proteins with enhanced controllability by NIR light, wherein such enhanced controllability exists when said fusion proteins have high ratios of activity in the light versus dark or vice versa. (patentsencyclopedia.com)
- transforming suitable organisms, with this DNA, expressing the DNA, and screening the resultant fusion proteins for additional fusion proteins controllable by NIR light. (patentsencyclopedia.com)
- Photosensitizing fluorescent proteins, which generate reactive oxygen species (ROS) upon light irradiation, are useful for spatiotemporal protein inactivation and cell ablation. (beds.ac.uk)
- Through absorption of the laser light, chromophores become excited, react with oxygen and generate reactive oxygen species (ROS) that, in turn, cause damage to DNA, RNA, lipids and proteins. (biologists.org)
Endogenous2
- 4. The method of claim 3 wherein said test organisms do not comprise an endogenous chromophore. (patentsencyclopedia.com)
- Natural organic matter (NOM) has a large influence, as it can attenuate radiation and thus decrease inactivation by endogenous mechanisms. (rsc.org)
Genetically4
- Genetically targeted chromophore-assisted light inactivation. (biomedcentral.com)
- Genetically-encoded photosensitizers produce reactive oxygen species (ROS) in response to light. (nih.gov)
- KillerOrange, a Genetically Encoded Photosensitizer Activated by Blue and Green Light. (evrogen.ru)
- The objective of this project is to address this challenge by developing an innovative, versatile, genetically-encoded method by which a protein of interest can be disrupted by specific light-activated proteolysis in either whole cells or intracellular regions as a novel tool to analyze protein function in live cells. (grantome.com)
Spatiotemporal3
- Since ROS generation of a photosensitizer is specifically controlled by certain excitation wavelengths, utilizing colour variants of photosensitizing protein would allow multi-spatiotemporal control of inactivation. (beds.ac.uk)
- As a proof of concept for multi-spatiotemporal control of inactivation, we demonstrate that SuperNova Green can be used with its red variant, SuperNova, to perform independent protein inactivation or cell ablation studies in a spatiotemporal manner by selective light irradiation. (beds.ac.uk)
- So, by using a focused laser beam and controlling the generation of ROS within a specific subcellular area with micrometer to sub-micrometer accuracy, the spatiotemporal inactivation of targets can be achieved. (biologists.org)
Irradiation2
- It uses light irradiation to generate a temporary toxic form of oxygen that causes an area of damage shorter than a typical protein-protein interaction distance. (eurekalert.org)
- SuperNova Green is able to produce ROS spatiotemporally upon blue light irradiation. (beds.ac.uk)
Photosensitizer2
- min/µmol photosensitizer for an excitation fluence rate of 5.9mW/mm 2 at 470 ± 20nm, and the rate remained consistent across fluences (light doses). (nih.gov)
- In the present work, researchers made an antibody against the outer part of the neurotransmitter receptor GluA1 that they labeled with a light-sensitive molecule (a photosensitizer). (eurekalert.org)
Photodynamic Therapy2
- Indwelling Stent Embedded with Light-Emitting Diodes for Photodynamic Therapy of Malignant Biliary Obstruction. (rochester.edu)
- SummaryDespite of the approval of Photofrin® in various countries, chemically defined sensitizers for photodynamic therapy (PDT) are still needed for the absorption of light in the infrared spectrum, which provides a maximal penetration of light into tissue. (semanticscholar.org)
Technique2
- Therefore, we sought a technique for rapid protein inactivation while carrying out live-cell imaging experiments to better understand Golgi dynamics. (biologists.org)
- This has been a demonstration of a trendy technique in neuroscience: optogenetics, controlling individual brain cells with finely-tuned wavelengths of light. (neuwritesd.org)
Synaptic1
- This showed that the fear memory had been erased by the inactivation of synaptic GluA1. (eurekalert.org)
Photocatalysts1
- The implications of this mechanistic understanding of solar inactivation are discussed for a range of applications, including recreational water quality, natural treatment systems, solar disinfection of drinking water (SODIS), and enhanced inactivation via the use of sensitizers and photocatalysts. (rsc.org)
Selective2
- Quantification of light-induced miniSOG superoxide production using the selective marker, 2-hydroxyethidium. (rochester.edu)
- Methods pertaining to the generation of new individuals, including techniques used in selective BREEDING, cloning (CLONING, ORGANISM), and assisted reproduction (REPRODUCTIVE TECHNIQUES, ASSISTED). (umassmed.edu)
Wavelengths of light2
- Different isomeric manifestations of the molecule absorbs different wavelengths of light, so that each isomeric species can display a different color based on its absorption. (wikipedia.org)
- These opsins are stimulated by different wavelengths of light, so they can be put in the same neuron, allowing us to effectively turn the neuron on and off at will. (neuwritesd.org)
Targets1
- In this critical review, we summarize the photo-physics, -chemistry, and -biology that underpin sunlight-mediated inactivation, as well as the targets of damage and cellular responses to sunlight exposure. (rsc.org)
Cell8
- Here, we describe the synthesis, characterization, and utility of two photosensitizers based upon the widely used rhodamine scaffold and demonstrate their efficacy for chromophore-assisted light inactivation, cell ablation in culture and in vivo, and photopolymerization of diaminobenzidine for electron microscopy. (janelia.org)
- After administration of a 100-ms dose of 1.5 mW/[μm]2 laser light, KDR cells exhibited dynamic changes on the dorsal surface of the cell, forming numerous protrusions as seen in this DIC time-lapse movie. (cellimagelibrary.org)
- Light-induced blockage of cell division with a chromatin-targeted phototoxic fluorescent protein. (evrogen.ru)
- In order to express a light-sensitive protein in neurons, a genetic construct containing the opsin and a protein that fluorescently tags the cell (in order to identify where it is expressed) is placed in a virus that is then injected into the region of interest in the brain. (neuwritesd.org)
- Inactivation of antigen MKI67 leads to inhibition of ribosomal RNA synthesis.The MKI67 protein is a nuclear and nucleolar protein, which is tightly associated with somatic cell proliferation. (sinobiological.com)
- Development of SuperNova Green has expanded the photosensitizing protein toolbox to optogenetically control protein inactivation and cell ablation. (beds.ac.uk)
- 3. The apparatus of claim 1 or 2 , whereby the focal volume comprises electromagnetic radiation sufficient to affect the cell of interest resulting in a cellular response selected from the group of cell death, transient permeabilization of the cell membrane, activation of a photosensitive agent, or inactivation of a cell component. (google.com)
- Cholesterol-Assisted Bacterial Cell Surface Engineering for Photodynamic Inactivation of Gram-Positive and Gram-Negative Bacteria. (semanticscholar.org)
Mammalian1
- In mammalian systems, few approaches allow acute inactivation and as a consequence the immediate effects of a blockade of membrane trafficking are largely unknown. (biologists.org)
Susceptible2
- Viruses that are not susceptible to exogenous inactivation are only inactivated if UVB wavelengths (280-320 nm) are present, such as in very clear, open waters or in containers that are transparent to UVB. (rsc.org)
- Some viruses and bacteria (especially Gram-positive) are susceptible to exogenous inactivation, which can be initiated by visible as well as UV wavelengths. (rsc.org)
Illumination3
- In response to illumination of the mouse hippocampus with green light, we found that mice returned to the dark boxes more quickly than control animals," study first author Kiwamu Takemoto says. (eurekalert.org)
- Because we propose to use light to toggle protease activity, experiments can be carried out entirely on an adequately equipped microscope allowing unprecedented high temporal and spatial control of intracellular protein inactivation by using patterned illumination. (grantome.com)
- To test and expand upon this concept, we monitored autofluorescence and light scattering intensities of injured vs. uninjured rat kidneys via multimodal imaging under 355 nm, 325 nm, and 266 nm excitation as well as scattering under 500 nm illumination. (spie.org)
Inhibition2
- Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis. (wikipedia.org)
- 2007) Chromophore-assisted light inactivation of pKi-67 leads to inhibition of ribosomal RNA synthesis. (sinobiological.com)
Organisms3
- 3. The method of claim 1 also comprising transforming said test organisms with DNA encoding a heme oxygenase gene capable of being expressed in said test organisms to produce a biliverdin IXquadrature chromophore. (patentsencyclopedia.com)
- We review approaches to model sunlight-mediated inactivation and illustrate how the environmental conditions can dramatically shift the inactivation rate of organisms. (rsc.org)
- 2 Sunlight is the key factor contributing to inactivation of indicator organisms and pathogens in engineered natural systems like wastewater treatment ponds (WTP) 3 and open-water wetlands for treatment of wastewater and stormwater. (rsc.org)
Demonstrate1
- In this project we not only plan to experimentally demonstrate the crucial role of the phonon-assisted dynamics in facilitating efficient energy transfer in chromophoric complexes and odour recognition, but also to develop a general theoretical framework to describe and understand the role coherent vibrations play in the dynamics of biomolecular systems, as well as to develop methods to identify the presence and properties of such vibrations. (thefreedictionary.com)
Processes2
- Such FPs can absorb visible day-light and predominantly one variant of the red fluorescent protein, the KillerRed protein (KRED) emits active electrons producing reactive oxygen species (ROS) leading to photokilling processes in eukaryotes. (medsci.org)
- To accurately predict inactivation and design engineered systems that enhance solar inactivation, it is necessary to model these processes, although some details are not yet sufficiently well understood. (rsc.org)
Absorption of li1
- Typical fluorescence microscopy techniques rely upon the absorption of light by a fluorophore at one wavelength (excitation), followed by the subsequent emission of secondary fluorescence at a longer wavelength. (zeiss.com)
Experiments1
- Photosensitizers, compounds that generate reactive oxygen species upon excitation with light, can be utilized for numerous biological experiments, but the repertoire of bioavailable photosensitizers is limited. (janelia.org)
Intense1
- The authors used chromophore-assisted laser inactivation to focus intense light from a laser on the MyoII cable in tissues from animals expressing nonmuscle MyoII regulatory light chain protein that was fused to green fluorescent protein (GFP). (sciencemag.org)
Precise1
- However, this is only informative if the inactivation is precise with respect to space and time. (eurekalert.org)
Excitation2
- Photosensitizers are chromophores which generate excessive reactive oxygen species (ROS) when irradiated by excitation light. (beds.ac.uk)
- Fluorescence spectra can be detected from dye molecules, and native molecules in tissues samples with excitation from wavelengths extracted from ultrafast SC light in the spectral range between 350 to 500 nm using narrow bandpass filters. (spie.org)
Techniques2
- To overcome these problems, the development of techniques that utilize a light-sensitive channel has been actively pursued. (ehime-u.ac.jp)
- Afin de comprendre comment les émotions sont encodées et transmises dans ces structures, et comment elles affectent le comportement, l'équipe du Pr Proulx utilise des techniques avancées (photométrie par fibre optique, mesures d'électrophysiologie, optogénétique) qui lui permettent de mesurer et de manipuler l'activité neuronale, en temps réel chez des animaux libres de mouvement. (ulaval.ca)
Accurately1
- For instance, in one embodiment, the reader can utilize a silicon photodiode and a pulsed light-emitting diode (LED) to accurately excite labels and detect fluorescence on a membrane-based assay device without requiring the use of expensive components, such as monochromators or narrow emission band width optical filters. (google.com.au)
Microscopy1
- Together, these new tools facilitate light-microscopy-based neuronal tracing at both a large scale and a high resolution. (elifesciences.org)
Absorb light3
- Normally, this substance would not be able to absorb light, but with the attached biosensor, light can be absorbed and emitted on a spectrophotometer. (wikipedia.org)
- Their ability to display different colors lies in how they absorb light. (wikipedia.org)
- Any of various chemical groups that absorb light of certain wavelengths and when present in an organic compound, such as a dye or pigment, are responsible for the color of the compound. (thefreedictionary.com)
Effectively1
- This opsin is then incorporated in the neuron's membrane, and a specific wavelength of light will prompt the opsin to open its ion channel, causing a depolarization of the membrane, effectively causing an action potential. (neuwritesd.org)
Laser light2
- Laser light was focused onto the specimen plane through a 488-nm laser filter set (Z488/10, HQ525/50, Z488RDC, Chroma Technology Corporation). (cellimagelibrary.org)
- Indocyanine green and laser light for the treatment of AIDS-associated cutaneous Kaposi's sarcoma. (semanticscholar.org)
Widely1
- The segment polarity genes that have been most widely studied in other insects are engrailed ( en ) and wingless ( wg ). (biologists.org)
Control2
- Remote Control of Light-Triggered Virotherapy. (evrogen.ru)
- Inactivation is accomplished using a device having a unique temperature control design. (google.ca)
Green2
- Two male mice are hanging out in a cage, both with strange looking cables coming out of their head, and both minding their own business, when all of a sudden, a researcher flips a switch, sending a green light down the cable and into one of the mice's brain. (neuwritesd.org)
- The first mouse has a specific channel that excites the neurons in response to green light, whereas the other has red light-responsive channels that silence the neuron when activated. (neuwritesd.org)
Exposure1
- Method of making images on a sensitized surface by exposure to light or other radiant energy. (childrensmercy.org)
Optical2
- Determination of optical properties by interstitial white light spectroscopy using a custom fiber optic probe. (rochester.edu)
- The light comes from either a laser via an optical fiber that runs into the brain, or a surgically implanted LED. (neuwritesd.org)