Chromomycins
Olivomycins
Chromomycin A3
Streptomyces
Acyl Carrier Protein
Streptomyces lividans
Streptomyces griseus
Dermoscopy
Lymph Nodes
Biopsy
Skin
Biopsy, Needle
Biopsy, Fine-Needle
Physical Examination
Inventions
Intellectual Property
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
Eye
Blood Vessels
Reproducibility of Results
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Liposomes
Azetidinecarboxylic Acid
Plicamycin
A tricyclic pentaglycosidic antibiotic from Streptomyces strains that inhibits RNA and protein synthesis by adhering to DNA. It is used as a fluorescent dye and as an antineoplastic agent, especially in bone and testicular tumors. Plicamycin is also used to reduce hypercalcemia, especially that due to malignancies.
Axinella
Rhabdomyosarcoma, Alveolar
A form of RHABDOMYOSARCOMA occurring mainly in adolescents and young adults, affecting muscles of the extremities, trunk, orbital region, etc. It is extremely malignant, metastasizing widely at an early stage. Few cures have been achieved and the prognosis is poor. "Alveolar" refers to its microscopic appearance simulating the cells of the respiratory alveolus. (Holland et al., Cancer Medicine, 3d ed, p2188)
Rhabdoid Tumor
A rare but highly lethal childhood tumor found almost exclusively in infants. Histopathologically, it resembles RHABDOMYOSARCOMA but the tumor cells are not of myogenic origin. Although it arises primarily in the kidney, it may be found in other parts of the body. The rhabdoid cytomorphology is believed to be the expression of a very primitive malignant cell. (From Holland et al., Cancer Medicine, 3d ed, p2210)
Rhabdomyosarcoma
A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9)
Rhabdomyosarcoma, Embryonal
A form of RHABDOMYOSARCOMA arising primarily in the head and neck, especially the orbit, of children below the age of 10. The cells are smaller than those of other rhabdomyosarcomas and are of two basic cell types: spindle cells and round cells. This cancer is highly sensitive to chemotherapy and has a high cure rate with multi-modality therapy. (From Holland et al., Cancer Medicine, 3d ed, p2188)
Mass Media
Paranasal Sinuses
Air-filled spaces located within the bones around the NASAL CAVITY. They are extensions of the nasal cavity and lined by the ciliated NASAL MUCOSA. Each sinus is named for the cranial bone in which it is located, such as the ETHMOID SINUS; the FRONTAL SINUS; the MAXILLARY SINUS; and the SPHENOID SINUS.
Interaction of the DNA-binding antitumor antibiotics, chromomycin and mithramycin with erythroid spectrin. (1/34)
The aureolic acid group of antitumor antibiotics, chromomycin A3 and mithramycin, are well established as transcription inhibitors, which bind reversibly to DNA at and above physiological pH, in the presence of divalent metal ions such as Mg2+. As part of our broad objective to elucidate their intracellular mode of action, other than association with DNA, we studied their interactions with the erythrocyte cytoskeletal protein, spectrin, in the absence and presence of magnesium. Different spectroscopic studies, such as absorbance, fluorescence and CD, have shown that both free chromomycin and mithramycin and their Mg2+ complexes bind to spectrin with an affinity higher than that reported for DNA. The affinity constants for the association of chromomycin and mithramycin (or their Mg2+ complexes) with spectrin are comparable with those for the association of spectrin with other cytoskeletal proteins, for example F-actin, ankyrin, protein 4.1, etc. The nature of the binding of the two antibiotics to spectrin is different. The mode of binding of the antibiotics with spectrin also changes in the presence of Mg2+. The interaction leads to a change in the tertiary structure of the protein. The relevance of the results to our understanding of the mode of action of the antibiotics is discussed. (+info)A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic resonance. (2/34)
BACKGROUND: The drug chromomycin-A(3) binds to the minor groove of DNA and requires a divalent metal ion for complex formation. (1)H, (31)P and (13)C pseudocontact shifts occurring in the presence of a tightly bound divalent cobalt ion in the complex between d(TTGGCCAA)(2) and chromomycin-A(3) have been used to determine the structure of the complex. The accuracy of the structure was verified by validation with nuclear Overhauser enhancements (NOEs) and J-coupling constants not used in the structure calculation. RESULTS: The final structure was determined to 0.7 A resolution. The structure was compared with a structure obtained in an earlier study using NOEs, in order to assess the accuracy of NOEs in giving global structural information for a DNA complex. Although some basic features of the structures agreed, they differed substantially in the fine structural details and in the DNA axis curvature generated by the drug. The distortion of base-pair planarity that was observed in the NOE structure was not seen in our structure. Differences in drug orientation and hydrogen bonding also occurred. The curvature and elongation of the DNA that was obtained previously was not found to occur in our study. CONCLUSIONS: The use of pseudocontact shifts has enabled us to obtain a high-precision global structure of the chromomycin-DNA complex, which provides an accurate template on which to consider targeting minor groove binding drugs. The effect of such binding is not propagated far along the helix but is restricted to a local kink in the axis that reverts to its original direction within four base pairs. (+info)Biospecific interaction analysis (BIA) of low-molecular weight DNA-binding drugs. (3/34)
DNA-binding drugs have been reported to be able to interfere with the activity of transcription factors in a sequence-dependent manner, leading to alteration of transcription. This and similar effects could have important practical applications in the experimental therapy of many human pathologies, including neoplastic diseases and viral infections. The analysis of the biological activity of DNA-binding drugs by footprinting, gel retardation, polymerase chain reaction, and in vitro transcription studies does not allow a real time study of binding to DNA and dissociation of the generated drugs/DNA complexes. The recent development of biosensor technologies for biospecific interaction analysis (BIA) enables monitoring of a variety of molecular reactions in real-time by surface plasmon resonance (SPR). In this study, we demonstrate that molecular interactions between DNA-binding drugs (chromomycin, mithramycin, distamycin, and MEN 10567) and biotinylated target DNA probes immobilized on sensor chips is detectable by SPR technology using a commercially available biosensor. The target DNA sequences were synthetic oligonucleotides mimicking the Sp1, NF-kB, and TFIID binding sites of the long terminal repeat of the human immunodeficiency type 1 virus. The results obtained demonstrate that mithramycin/DNA complexes are less stable than chromomycin/DNA complexes; distamycin binds to both NF-kB and TATA box oligonucleotides, but distamycin/(NF-kB)DNA complexes are not stable; the distamycin analog MEN 10567 binds to the NF-kB mer and the generated drug/DNA complexes are stable. The experimental approach described in this study allows fast analysis of molecular interactions between DNA-binding drugs and selected target DNA sequences. Therefore, this method could be used to identify new drugs exhibiting differential binding activities to selected regions of viral and eukaryotic gene promoters. (+info)SAF-Box, a conserved protein domain that specifically recognizes scaffold attachment region DNA. (4/34)
SARs (scaffold attachment regions) are candidate DNA elements for partitioning eukaryotic genomes into independent chromatin loops by attaching DNA to proteins of a nuclear scaffold or matrix. The interaction of SARs with the nuclear scaffold is evolutionarily conserved and appears to be due to specific DNA binding proteins that recognize SARs by a mechanism not yet understood. We describe a novel, evolutionarily conserved protein domain that specifically binds to SARs but is not related to SAR binding motifs of other proteins. This domain was first identified in human scaffold attachment factor A (SAF-A) and was thus designated SAF-Box. The SAF-Box is present in many different proteins ranging from yeast to human in origin and appears to be structurally related to a homeodomain. We show here that SAF-Boxes from four different origins, as well as a synthetic SAF-Box peptide, bind to natural and artificial SARs with high specificity. Specific SAR binding of the novel domain is achieved by an unusual mass binding mode, is sensitive to distamycin but not to chromomycin, and displays a clear preference for long DNA fragments. This is the first characterization of a specific SAR binding domain that is conserved throughout evolution and has DNA binding properties that closely resemble that of the unfractionated nuclear scaffold. (+info)Effect of the antitumor antibiotic chromomycin A3 on the humoral immune response in rats. (5/34)
Chromomycin A3 (250 mug/kg) suppressed the humoral immune response in rats against sheep erythrocytes when administered 48 h or later after antigenic stimulus. The antibiotic at this dose enhanced immunity when given along with or before antigen administration. The natural heterohemagglutinin levels in rabbits and guinea pigs were not affected by the antibiotic (10 mug/kg per day x 7). (+info)In vivo topoisomerase II cleavage of the Drosophila histone and satellite III repeats: DNA sequence and structural characteristics. (6/34)
We have identified two classes of in vivo topoisomerase II cleavage sites in the Drosophila histone gene repeat. One class co-localizes with DNase I-hypersensitive regions and another novel class maps to a subset of consecutive nucleosome linker sites in the scaffold-associated region (SAR) of the histone gene loop. Prominent topoisomerase II cleavage is also observed in one of the linker regions of the two nucleosomes spanning satellite III, a centromeric SAR-like DNA sequence with a repeat length of 359 bp. At the sequence level, in vivo topoisomerase II cleavage is highly site specific. Comparison of 10 nucleosome linker sites defines an in vivo cleavage sequence whose major characteristic is a prominent GC-rich core. These GC-rich cleavage sites are flanked by extensive arrays of oligo(dA).oligo(dT) tracts characteristic of SAR sequences. Treatment of cells with distamycin selectively enhances cleavage at nucleosome linker sites of the SAR and satellite regions, suggesting that AT-rich sequences flanking cleavage sites may be involved in determining topoisomerase II activity in the cell. These observations provide evidence for the association of topoisomerase II with SARS in vivo. (+info)Aureolic acids: similar antibiotics with different biosynthetic gene clusters. (7/34)
In this issue of Chemistry & Biology, Mendez and colleagues describe the sequence and organization of the chromomycin gene cluster. Unexpectedly, the arrangement is starkly different from the mithramycin biosynthetic cluster, despite similarity in the individual genes and the near identical structures of the two antibiotic aureolic acids. (+info)Variation in chromosome numbers, CMA bands and 45S rDNA sites in species of Selaginella (Pteridophyta). (8/34)
BACKGROUND AND AIMS: Selaginella is the largest genus of heterosporous pteridophytes, but karyologically the genus is known only by the occurrence of a dysploid series of n=7-12, and a low frequency of polyploids. Aiming to contribute to a better understanding of the structural chromosomal variability of this genus, different staining methods were applied in species with different chromosome numbers. METHODS: The chromosome complements of seven species of Selaginella were analysed and, in four of them, the distribution of 45S rDNA sites was determined by fluorescent in situ hybridization. Additionally, CMA/DA/DAPI and silver nitrate staining were performed to investigate the correlation between the 45S rDNA sites, the heterochromatic bands and the number of active rDNA sites. KEY RESULTS: The chromosome numbers observed were 2n=18, 20 and 24. The species with 2n=20 exhibited chromosome complement sizes smaller and less variable than those with 2n=18. The only species with 2n=24, S. convoluta, had relatively large and asymmetrical chromosomes. The interphase nuclei in all species were of the chromocentric type. CMA/DA/DAPI staining showed only a weak chromosomal differentiation of heterochromatic bands. In S. willdenowii and S. convoluta eight and six CMA+ bands were observed, respectively, but no DAPI+ bands. The CMA+ bands corresponded in number, size and location to the rDNA sites. In general, the number of rDNA sites correlated with the maximum number of nucleoli per nucleus. Ten rDNA sites were found in S. plana (2n=20), eight in S. willdenowii (2n=18), six in S. convoluta (2n=24) and two in S. producta (2n=20). CONCLUSIONS: The remarkable variation in chromosome size and number and rDNA sites shows that dramatic karyological changes have occurred during the evolution of the genus at the diploid level. These data further suggest that the two putative basic numbers of the genus, x=9 and x=10, may have arisen two or more times independently. (+info)
Manipulation of two regulatory genes for efficient production of chromomycins in Streptomyces reseiscleroticus | Journal of...
Aureolic acid synonyms, aureolic acid antonyms - FreeThesaurus.com
Comparative studies on histology and histochemistry of pancreas between Labeo calbasu (Hamilton, 1822) and Mystus gulio ...
https://www.thefreelibrary.com/Taxonomic+Characterization+and+Antagonistic+Efficacy+of+Streptomyces...-a0573094538
KAKEN - Research Projects | 1997 Fiscal Year Final Research Report Summary (KAKENHI-PROJECT-08680820)
FDA approves vaccine to block meningitis strain - AP News - Breaking News
The aureolic acid family of antitumor compounds: structure, mode of action, biosynthesis, and novel derivatives
Heronamide C
RCSB PDB
- 127D: DNA (5-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3) COMPLEXED WITH HOECHST 33258 Methods Report Page
KGS--Subsurface Mississippian Rocks--Fossils
Quantitative Footprinting Analysis of the Chromomycin A<sub>3</sub>-DNA...
Quantitative footprinting analysis of the chromomycin A3-DNA interaction<...
Cytotoxic and antimicrobial napyradiomycins from two marine-derived streptomyces strains | Scripps Institution of Oceanography,...
In silico, spectroscopic, and biological insights on annelated pyrrolo[3,2-e]pyrimidines with antiproliferative activity<...
RCSB PDB
- 1EKH: NMR STRUCTURE OF D(TTGGCCAA)2 BOUND TO CHROMOMYCIN-A3 AND COBALT Macromolecule Annotations Page
Biblio | Scripps Institution of Oceanography, UC San Diego
Chro 00340 : CDS information --- DoBISCUIT
PKS BLAST table : Acla 00190
Synthesis and DNA-binding properties of bisdiazoliumylporphyrins<...
Biospecific Affinity Chromatography of Sweet-Potato β-Amylase | Biochemical Society Transactions | Portland Press
Mithramycin - definition of mithramycin by The Free Dictionary
Mg2+ Mediated Sequence-Specific Binding of Transcriptional Activator Protein C of Bacteriophage Mu to DNA - [email protected]
Plicamycin - Wikipedia
Departament of Producers Mutagenesis and Strain Improvement | FSBI Gause Institute of New Antibiotics
1d90 - Proteopedia, life in 3D
Scientific Protocols -
Peptide nucleic acid (PNA) fluorescent in situ hybridization (FISH) on chromosomes in suspension...
KLF7 Polyclonal Antibody - Abbkine - Antibodies, proteins, biochemicals, assay kits for life science research
DSpace at EWHA: 항암 효과를 갖는 2-phenylquinazolin-4(3H)-one 유도체의 합성과 활성 연구
Biospecific binding to immobilized small ligands in affinity chromatography | Biochemical Society Transactions
B3GZ69 | SWISS-MODEL Repository
B3H073 | SWISS-MODEL Repository
Cervical cancer: Approach to Care | UF Health, University of Florida Health
Division of Female Pelvic Medicine and Reconstructive Surgery | CRSLS
Determination of pseudocontact shifts of low-populated excited states by NMR chemical exchange saturation transfer - Physical...
Interview on recent Protest in Poland | anarchistnews.org
Synthesis of Multifunctional Polyvinylsaccharide Containing Controllable Amounts of Biospecific Ligands
Rita NaSiak, Bangga Terlibat Di Kompetisi CBD TVRI Riau-Kepri - Dangdut Jempol
Articles about Rita Moreno - tribunedigital-orlandosentinel
Lirik dan Kord Kunci Gitar Ajojing - Rhoma Irama & Rita Sugiarto ~ Maingitardulu.com
Molecular Cloning
Melanin in Fonsecaea pedrosoi : a trap for oxidative radicals | BMC Microbiology | Full Text
Immunological cross reaction between calf and Drosophila histones<...
Breeding seasonality and population dynamics of the catfish Schilbe mystus (Schilbeidae) in the Cross River, Nigeria
|...
New Coating from DataLase Provides High Humidity Solution | DPS Magazine
Rita Levi-Montalcini Obituary: Rita Levi-Montalcinis Obituary by the The Record/Herald News.
References Used for Corica soborna
herbal medicine
Rita update - The Blessing of Animal Companions
WHAT IS WRONG WITH HUMAN TRAFFICKING?. CRITICAL PERSPECTIVES ON THE LAW. RITA HAVERKAMP;ESTER HERLIN-KARNELL;CLAES LERNESTEDT....
Unraveling the karyotype structure of the spurges Euphorbia hirta Linnaeus, 1753 and E. hyssopifolia Linnaeus, 1753 ...
Study of the conidial development and cleistothecium-like structure of some strains of Fonsecaea pedrosoi | SpringerLink
Rita Levi-Montalcini Obituary: Rita Levi-Montalcinis Obituary by the Chicago Sun-Times.
RITA (NSC 652287) | p53 activator | Buy RITA (NSC 652287) from Supplier AdooQ®
Rita Ora - Page 12
124 Rita Rd, New Castle, DE 19720 - 195307027 | RealtyTrac
Educating Rita | The List
Marine Drugs | Free Full-Text | Chromomycins A2 and A3 from Marine Actinomycetes with TRAIL Resistance-Overcoming and Wnt...
To the best of our knowledge, the effects of chromomycins A2 (1) and A3 (2) on TRAIL resistance-overcoming activity, and on the ... led to the isolation of chromomycins A2 (1) and A3 (2). 1 and 2 showed potent cytotoxicity against the human gastric ... To the best of our knowledge, the effects of chromomycins A2 (1) and A3 (2) on TRAIL resistance-overcoming activity, and on the ... Chromomycins A2 and A3 from Marine Actinomycetes with TRAIL Resistance-Overcoming and Wnt Signal Inhibitory Activities. ...
Manipulation of two regulatory genes for efficient production of chromomycins in Streptomyces reseiscleroticus | Journal of...
Chromomycins are promising anticancer natural products from actinomycetes. This study is aimed to create an efficient strain ... Optimization of the culture conditions further increased the titers of chromomycins A3 and A2 respectively to 145.1 ± 15.3 and ... The resulting engineered strain showed the highest production titers of chromomycins by a strain of Streptomyces, providing an ... Either overexpression of SrcmRI or disruption of SrcmRII turned on the biosynthetic pathway of chromomycins. The production ...
Epirubicin
DHAD - Drug Information - Chemocare
DaunoXome - Drug Information - Chemocare
Actinomycetes from solitary wasp mud nest and swallow bird mud nest: isolation and screening for their antibacterial activity |...
High resolution physical mapping of 45S (5.8S, 18S and 25S) rDNA gene loci in the tomato genome using a combination of...
Frontiers | Targeting β-Catenin Signaling by Natural Products for Cancer Prevention and Therapy | Pharmacology
2017 - Winship Health Institute
Winship Health Institute - Your #1 Source on Preventive Health
Patent US6248727 - Selective and non-invasive visualization or treatment of vasculature - Google Patents
Olivomycins
Summary Report | CureHunter
WO2008064425A1 - Glycoalkaloid and chemotherapeutic agent combinations and various uses thereof
- Google Patents
The aureolic acid family of antitumor compounds: structure, mode of action, biosynthesis, and novel derivatives
Use of Cxcr4 Protein Expression on the Surface of Stem Cells as a Marker for Tumor Tropic Potential - CEDARS-SINAI MEDICAL...
Extracorporeal Removal of Microvesicular Particles - AETHLON MEDICAL, INC.
Targeting the Oncogenic TBX2 Transcription Factor ... - BV FAPESP
Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins. FRONTIERS IN CHEMISTRY 8 n. p. MAR 3 2020. Artigo ... Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins Texto completo Autor(es):. Mostrar menos -. Sahm, Bianca ... Here, we adopt a targeted approach based on a reverse-affinity procedure to identify the ability of chromomycins A(5) (CA(5)) ...
Koji Nakanishi - Publications
WO2003000236A1 - Particles with improved solubilization capacity
- Google Patents
Application # 2018/0134794. ANTI-MET ANTIBODIES, BISPECIFIC ANTIGEN BINDING MOLECULES THAT BIND MET,
AND METHODS OF USE...
Rare Cancer News & Clinical Trials » PubMed - Rhabdomyosarcoma
"Investigation and Engineering of Polyketide Biosynthetic Pathways" by Lei Sun
... and optimized the culture conditions to increase the titer of chromomycins. The production of emodin nowadays mostly relies on ... The engineered Streptomyces roseiscleroticus strain constructed in this work showed higher titers of chromomycins than ... The engineered Streptomyces roseiscleroticus strain constructed in this work showed higher titers of chromomycins than ... and optimized the culture conditions to increase the titer of chromomycins. The production of emodin nowadays mostly relies on ...
Find Scholarly Works
- Arizona State University
Hadimani, M. B., MacDonough, M. T., Ghatak, A., Strecker, T. E., Lopez, R., Sriram, M., Nguyen, B. L., Hall, J. J., Kessler, R. J., Shirali, A. R., Liu, L., Garner, C. M., Pettit, G., Hamel, E., Chaplin, D. J., Mason, R. P., Trawick, M. L. & Pinney, K. G., Sep 27 2013, In : Journal of Natural Products. 76, 9, p. 1668-1678 11 p.. Research output: Contribution to journal › Article ...
DeCS
US Patent Application for METHOD FOR PROTECTION OF ANTIMICROBIAL AND ANTICANCER DRUGS FROM INACTIVATION BY NITRIC OXIDE Patent...
Canada Online Drugstore: Dostinex price any pills online!
Can these lengthy times in between making him more susceptible to uti's ? - lookformedical.com
Preparation and bivariate analysis of suspensions of human chromosomes<...
Leticia Veras Costa Lotufo - Research Supported by FAPESP
Titers of chromomycins3
- Optimization of the culture conditions further increased the titers of chromomycins A 3 and A 2 respectively to 145.1 ± 15.3 and 158.3 ± 15.4 mg/L in liquid fermentation. (biomedcentral.com)
- The resulting engineered strain showed the highest production titers of chromomycins by a strain of Streptomyces , providing an efficient way to produce these pharmaceutically valuable molecules. (biomedcentral.com)
- The engineered Streptomyces roseiscleroticus strain constructed in this work showed higher titers of chromomycins than previously reported, which was achieved by characterizing and engineering the chromomycin biosynthetic gene cluster. (usu.edu)
Mithramycin1
- Mithramycin and chromomycins are the most representative members of the family, mithramycin being used as a chemotherapeutic agent for the treatment of several cancer diseases. (nih.gov)
Strain2
- Cytotoxicity-guided fractionation of the CKK1019 strain culture broth, which exhibited cytotoxicity, led to the isolation of chromomycins A 2 ( 1 ) and A 3 ( 2 ). (mdpi.com)
- An engineered strain was then constructed with both SrcmRII disruption and SrcmRI overexpression, which produced chromomycins A 3 and A 2 in R5 agar at 69.4 ± 7.6 and 81.7 ± 7.2 mg/L, respectively. (biomedcentral.com)
Actinomycetes2
- Toume K, Tsukahara K, Ito H, Arai MA, Ishibashi M. Chromomycins A 2 and A 3 from Marine Actinomycetes with TRAIL Resistance-Overcoming and Wnt Signal Inhibitory Activities. (mdpi.com)
- Chromomycins are promising anticancer natural products from actinomycetes. (biomedcentral.com)
Culture conditions1
- I activated the polyketide biosynthetic pathway by engineering two regulatory genes, and optimized the culture conditions to increase the titer of chromomycins. (usu.edu)