A complex of several closely related glycosidic antibiotics from Streptomyces griseus. The major component, CHROMOMYCIN A3, is used as a fluorescent stain of DNA where it attaches and inhibits RNA synthesis. It is also used as an antineoplastic agent, especially for solid tumors.
A mixture of several closely related glycosidic antibiotics obtained from Actinomyces (or Streptomyces) olivoreticuli. They are used as fluorescent dyes that bind to DNA and prevent both RNA and protein synthesis and are also used as antineoplastic agents.
Glycosidic antibiotic from Streptomyces griseus used as a fluorescent stain of DNA and as an antineoplastic agent.
A genus of bacteria that form a nonfragmented aerial mycelium. Many species have been identified with some being pathogenic. This genus is responsible for producing a majority of the ANTI-BACTERIAL AGENTS of practical value.
Consists of a polypeptide chain and 4'-phosphopantetheine linked to a serine residue by a phosphodiester bond. Acyl groups are bound as thiol esters to the pantothenyl group. Acyl carrier protein is involved in every step of fatty acid synthesis by the cytoplasmic system.
An actinomycete used for production of commercial ANTIBIOTICS and as a host for gene cloning.
An actinomycete from which the antibiotics STREPTOMYCIN, grisein, and CANDICIDIN are obtained.
A noninvasive technique that enables direct microscopic examination of the surface and architecture of the SKIN.
They are oval or bean shaped bodies (1 - 30 mm in diameter) located along the lymphatic system.
Removal and pathologic examination of specimens in the form of small pieces of tissue from the living body.
The outer covering of the body that protects it from the environment. It is composed of the DERMIS and the EPIDERMIS.
Tumors or cancer of the SKIN.
Removal and examination of tissue obtained through a transdermal needle inserted into the specific region, organ, or tissue being analyzed.
Using fine needles (finer than 22-gauge) to remove tissue or fluid specimens from the living body for examination in the pathology laboratory and for disease diagnosis.
Services designed for HEALTH PROMOTION and prevention of disease.
Systematic and thorough inspection of the patient for physical signs of disease or abnormality.
Exclusive legal rights or privileges applied to inventions, plants, etc.
A novel composition, device, or process, independently conceived de novo or derived from a pre-existing model.
Property, such as patents, trademarks, and copyright, that results from creative effort. The Patent and Copyright Clause (Art. 1, Sec. 8, cl. 8) of the United States Constitution provides for promoting the progress of science and useful arts by securing for limited times to authors and inventors, the exclusive right to their respective writings and discoveries. (From Black's Law Dictionary, 5th ed, p1014)
The organ of sight constituting a pair of globular organs made up of a three-layered roughly spherical structure specialized for receiving and responding to light.
Any of the tubular vessels conveying the blood (arteries, arterioles, capillaries, venules, and veins).
The statistical reproducibility of measurements (often in a clinical context), including the testing of instrumentation or techniques to obtain reproducible results. The concept includes reproducibility of physiological measurements, which may be used to develop rules to assess probability or prognosis, or response to a stimulus; reproducibility of occurrence of a condition; and reproducibility of experimental results.
Artificial, single or multilaminar vesicles (made from lecithins or other lipids) that are used for the delivery of a variety of biological molecules or molecular complexes to cells, for example, drug delivery and gene transfer. They are also used to study membranes and membrane proteins.
A proline analog that acts as a stoichiometric replacement of proline. It causes the production of abnormal proteins with impaired biological activity.
A tricyclic pentaglycosidic antibiotic from Streptomyces strains that inhibits RNA and protein synthesis by adhering to DNA. It is used as a fluorescent dye and as an antineoplastic agent, especially in bone and testicular tumors. Plicamycin is also used to reduce hypercalcemia, especially that due to malignancies.
A genus of SPONGES in the family Axinellidae, comprised of a choanosomal skeleton differentiated in the axial and extra-axial region. The type species is Axinella polypoides.
Substances that inhibit or prevent the proliferation of NEOPLASMS.
A form of RHABDOMYOSARCOMA occurring mainly in adolescents and young adults, affecting muscles of the extremities, trunk, orbital region, etc. It is extremely malignant, metastasizing widely at an early stage. Few cures have been achieved and the prognosis is poor. "Alveolar" refers to its microscopic appearance simulating the cells of the respiratory alveolus. (Holland et al., Cancer Medicine, 3d ed, p2188)
A rare but highly lethal childhood tumor found almost exclusively in infants. Histopathologically, it resembles RHABDOMYOSARCOMA but the tumor cells are not of myogenic origin. Although it arises primarily in the kidney, it may be found in other parts of the body. The rhabdoid cytomorphology is believed to be the expression of a very primitive malignant cell. (From Holland et al., Cancer Medicine, 3d ed, p2210)
A malignant solid tumor arising from mesenchymal tissues which normally differentiate to form striated muscle. It can occur in a wide variety of sites. It is divided into four distinct types: pleomorphic, predominantly in male adults; alveolar (RHABDOMYOSARCOMA, ALVEOLAR), mainly in adolescents and young adults; embryonal (RHABDOMYOSARCOMA, EMBRYONAL), predominantly in infants and children; and botryoidal, also in young children. It is one of the most frequently occurring soft tissue sarcomas and the most common in children under 15. (From Dorland, 27th ed; Holland et al., Cancer Medicine, 3d ed, p2186; DeVita Jr et al., Cancer: Principles & Practice of Oncology, 3d ed, pp1647-9)
A form of RHABDOMYOSARCOMA arising primarily in the head and neck, especially the orbit, of children below the age of 10. The cells are smaller than those of other rhabdomyosarcomas and are of two basic cell types: spindle cells and round cells. This cancer is highly sensitive to chemotherapy and has a high cure rate with multi-modality therapy. (From Holland et al., Cancer Medicine, 3d ed, p2188)
Tumors or cancer of the PARANASAL SINUSES.
Instruments or technological means of communication that reach large numbers of people with a common message: press, radio, television, etc.
Air-filled spaces located within the bones around the NASAL CAVITY. They are extensions of the nasal cavity and lined by the ciliated NASAL MUCOSA. Each sinus is named for the cranial bone in which it is located, such as the ETHMOID SINUS; the FRONTAL SINUS; the MAXILLARY SINUS; and the SPHENOID SINUS.

Interaction of the DNA-binding antitumor antibiotics, chromomycin and mithramycin with erythroid spectrin. (1/34)

The aureolic acid group of antitumor antibiotics, chromomycin A3 and mithramycin, are well established as transcription inhibitors, which bind reversibly to DNA at and above physiological pH, in the presence of divalent metal ions such as Mg2+. As part of our broad objective to elucidate their intracellular mode of action, other than association with DNA, we studied their interactions with the erythrocyte cytoskeletal protein, spectrin, in the absence and presence of magnesium. Different spectroscopic studies, such as absorbance, fluorescence and CD, have shown that both free chromomycin and mithramycin and their Mg2+ complexes bind to spectrin with an affinity higher than that reported for DNA. The affinity constants for the association of chromomycin and mithramycin (or their Mg2+ complexes) with spectrin are comparable with those for the association of spectrin with other cytoskeletal proteins, for example F-actin, ankyrin, protein 4.1, etc. The nature of the binding of the two antibiotics to spectrin is different. The mode of binding of the antibiotics with spectrin also changes in the presence of Mg2+. The interaction leads to a change in the tertiary structure of the protein. The relevance of the results to our understanding of the mode of action of the antibiotics is discussed.  (+info)

A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic resonance. (2/34)

BACKGROUND: The drug chromomycin-A(3) binds to the minor groove of DNA and requires a divalent metal ion for complex formation. (1)H, (31)P and (13)C pseudocontact shifts occurring in the presence of a tightly bound divalent cobalt ion in the complex between d(TTGGCCAA)(2) and chromomycin-A(3) have been used to determine the structure of the complex. The accuracy of the structure was verified by validation with nuclear Overhauser enhancements (NOEs) and J-coupling constants not used in the structure calculation. RESULTS: The final structure was determined to 0.7 A resolution. The structure was compared with a structure obtained in an earlier study using NOEs, in order to assess the accuracy of NOEs in giving global structural information for a DNA complex. Although some basic features of the structures agreed, they differed substantially in the fine structural details and in the DNA axis curvature generated by the drug. The distortion of base-pair planarity that was observed in the NOE structure was not seen in our structure. Differences in drug orientation and hydrogen bonding also occurred. The curvature and elongation of the DNA that was obtained previously was not found to occur in our study. CONCLUSIONS: The use of pseudocontact shifts has enabled us to obtain a high-precision global structure of the chromomycin-DNA complex, which provides an accurate template on which to consider targeting minor groove binding drugs. The effect of such binding is not propagated far along the helix but is restricted to a local kink in the axis that reverts to its original direction within four base pairs.  (+info)

Biospecific interaction analysis (BIA) of low-molecular weight DNA-binding drugs. (3/34)

DNA-binding drugs have been reported to be able to interfere with the activity of transcription factors in a sequence-dependent manner, leading to alteration of transcription. This and similar effects could have important practical applications in the experimental therapy of many human pathologies, including neoplastic diseases and viral infections. The analysis of the biological activity of DNA-binding drugs by footprinting, gel retardation, polymerase chain reaction, and in vitro transcription studies does not allow a real time study of binding to DNA and dissociation of the generated drugs/DNA complexes. The recent development of biosensor technologies for biospecific interaction analysis (BIA) enables monitoring of a variety of molecular reactions in real-time by surface plasmon resonance (SPR). In this study, we demonstrate that molecular interactions between DNA-binding drugs (chromomycin, mithramycin, distamycin, and MEN 10567) and biotinylated target DNA probes immobilized on sensor chips is detectable by SPR technology using a commercially available biosensor. The target DNA sequences were synthetic oligonucleotides mimicking the Sp1, NF-kB, and TFIID binding sites of the long terminal repeat of the human immunodeficiency type 1 virus. The results obtained demonstrate that mithramycin/DNA complexes are less stable than chromomycin/DNA complexes; distamycin binds to both NF-kB and TATA box oligonucleotides, but distamycin/(NF-kB)DNA complexes are not stable; the distamycin analog MEN 10567 binds to the NF-kB mer and the generated drug/DNA complexes are stable. The experimental approach described in this study allows fast analysis of molecular interactions between DNA-binding drugs and selected target DNA sequences. Therefore, this method could be used to identify new drugs exhibiting differential binding activities to selected regions of viral and eukaryotic gene promoters.  (+info)

SAF-Box, a conserved protein domain that specifically recognizes scaffold attachment region DNA. (4/34)

SARs (scaffold attachment regions) are candidate DNA elements for partitioning eukaryotic genomes into independent chromatin loops by attaching DNA to proteins of a nuclear scaffold or matrix. The interaction of SARs with the nuclear scaffold is evolutionarily conserved and appears to be due to specific DNA binding proteins that recognize SARs by a mechanism not yet understood. We describe a novel, evolutionarily conserved protein domain that specifically binds to SARs but is not related to SAR binding motifs of other proteins. This domain was first identified in human scaffold attachment factor A (SAF-A) and was thus designated SAF-Box. The SAF-Box is present in many different proteins ranging from yeast to human in origin and appears to be structurally related to a homeodomain. We show here that SAF-Boxes from four different origins, as well as a synthetic SAF-Box peptide, bind to natural and artificial SARs with high specificity. Specific SAR binding of the novel domain is achieved by an unusual mass binding mode, is sensitive to distamycin but not to chromomycin, and displays a clear preference for long DNA fragments. This is the first characterization of a specific SAR binding domain that is conserved throughout evolution and has DNA binding properties that closely resemble that of the unfractionated nuclear scaffold.  (+info)

Effect of the antitumor antibiotic chromomycin A3 on the humoral immune response in rats. (5/34)

Chromomycin A3 (250 mug/kg) suppressed the humoral immune response in rats against sheep erythrocytes when administered 48 h or later after antigenic stimulus. The antibiotic at this dose enhanced immunity when given along with or before antigen administration. The natural heterohemagglutinin levels in rabbits and guinea pigs were not affected by the antibiotic (10 mug/kg per day x 7).  (+info)

In vivo topoisomerase II cleavage of the Drosophila histone and satellite III repeats: DNA sequence and structural characteristics. (6/34)

We have identified two classes of in vivo topoisomerase II cleavage sites in the Drosophila histone gene repeat. One class co-localizes with DNase I-hypersensitive regions and another novel class maps to a subset of consecutive nucleosome linker sites in the scaffold-associated region (SAR) of the histone gene loop. Prominent topoisomerase II cleavage is also observed in one of the linker regions of the two nucleosomes spanning satellite III, a centromeric SAR-like DNA sequence with a repeat length of 359 bp. At the sequence level, in vivo topoisomerase II cleavage is highly site specific. Comparison of 10 nucleosome linker sites defines an in vivo cleavage sequence whose major characteristic is a prominent GC-rich core. These GC-rich cleavage sites are flanked by extensive arrays of oligo(dA).oligo(dT) tracts characteristic of SAR sequences. Treatment of cells with distamycin selectively enhances cleavage at nucleosome linker sites of the SAR and satellite regions, suggesting that AT-rich sequences flanking cleavage sites may be involved in determining topoisomerase II activity in the cell. These observations provide evidence for the association of topoisomerase II with SARS in vivo.  (+info)

Aureolic acids: similar antibiotics with different biosynthetic gene clusters. (7/34)

In this issue of Chemistry & Biology, Mendez and colleagues describe the sequence and organization of the chromomycin gene cluster. Unexpectedly, the arrangement is starkly different from the mithramycin biosynthetic cluster, despite similarity in the individual genes and the near identical structures of the two antibiotic aureolic acids.  (+info)

Variation in chromosome numbers, CMA bands and 45S rDNA sites in species of Selaginella (Pteridophyta). (8/34)

BACKGROUND AND AIMS: Selaginella is the largest genus of heterosporous pteridophytes, but karyologically the genus is known only by the occurrence of a dysploid series of n=7-12, and a low frequency of polyploids. Aiming to contribute to a better understanding of the structural chromosomal variability of this genus, different staining methods were applied in species with different chromosome numbers. METHODS: The chromosome complements of seven species of Selaginella were analysed and, in four of them, the distribution of 45S rDNA sites was determined by fluorescent in situ hybridization. Additionally, CMA/DA/DAPI and silver nitrate staining were performed to investigate the correlation between the 45S rDNA sites, the heterochromatic bands and the number of active rDNA sites. KEY RESULTS: The chromosome numbers observed were 2n=18, 20 and 24. The species with 2n=20 exhibited chromosome complement sizes smaller and less variable than those with 2n=18. The only species with 2n=24, S. convoluta, had relatively large and asymmetrical chromosomes. The interphase nuclei in all species were of the chromocentric type. CMA/DA/DAPI staining showed only a weak chromosomal differentiation of heterochromatic bands. In S. willdenowii and S. convoluta eight and six CMA+ bands were observed, respectively, but no DAPI+ bands. The CMA+ bands corresponded in number, size and location to the rDNA sites. In general, the number of rDNA sites correlated with the maximum number of nucleoli per nucleus. Ten rDNA sites were found in S. plana (2n=20), eight in S. willdenowii (2n=18), six in S. convoluta (2n=24) and two in S. producta (2n=20). CONCLUSIONS: The remarkable variation in chromosome size and number and rDNA sites shows that dramatic karyological changes have occurred during the evolution of the genus at the diploid level. These data further suggest that the two putative basic numbers of the genus, x=9 and x=10, may have arisen two or more times independently.  (+info)

Regulatory genes play critical roles in natural product biosynthetic pathways. Chromomycins are promising anticancer natural products from actinomycetes. This study is aimed to create an efficient strain for production of these molecules by manipulating the regulatory genes. A putative but silent chromomycin biosynthetic gene cluster was discovered in Streptomyces reseiscleroticus. Heterologous expression of the ketosynthase, chain length factor, and acyl carrier protein in Streptomyces lividans confirmed that they are responsible for the assembly of a decaketide. Two regulatory genes are present in this gene cluster, including SARP-type activator SrcmRI and PadR-like repressor SrcmRII. Either overexpression of SrcmRI or disruption of SrcmRII turned on the biosynthetic pathway of chromomycins. The production titers of chromomycin A3/A2 in R5 agar in these two strains reached 8.9 ± 1.2/13.2 ± 1.6 and 49.3 ± 4.3/53.3 ± 3.6 mg/L, respectively. An engineered strain was then constructed with both SrcmRII
Synonyms for aureolic acid in Free Thesaurus. Antonyms for aureolic acid. 1 synonym for mithramycin: Mithracin. What are synonyms for aureolic acid?
Comparative studies on histology and histochemistry of pancreas between Labeo calbasu (Hamilton, 1822) and Mystus gulio (Hamilton, 1822)
Free Online Library: Taxonomic Characterization and Antagonistic Efficacy of Streptomyces cavourensis SKCMM1 Isolated from Sediment of Pichavaram Mangrove Forest.(RESEARCH ARTICLE, Report) by Journal of Pure and Applied Microbiology; Science and technology, general Amino acids Analysis Esters Fatty acids Mangrove swamps Environmental aspects Plant metabolites Sediments (Geology) Identification and classification Physiological aspects
FDA approves vaccine to block meningitis strain - AP News: WASHINGTON (AP) - Federal health regulators have approved the first .01/18/2018 3:26:41AM EST.
Members of the aureolic acid family are tricyclic polyketides with antitumor activity which are produced by different streptomycete species. These members are glycosylated compounds with two oligosaccharide chains of variable sugar length. They interact with the DNA minor groove in high-GC-content r …
Heronamides A - C, new polyketide macrolactams from an Australian marine-derived Streptomyces sp. A biosynthetic case for synchronized tandem electrocyclization. Ritesh R. et al. Org. Biomol. Chem. 2010, 8, 4682.. ...
127D: Conformation of B-DNA containing O6-ethyl-G-C base pairs stabilized by minor groove binding drugs: molecular structure of d(CGC[e6G]AATTCGCG complexed with Hoechst 33258 or Hoechst 33342.
The five samples 6480-6464 (6473-6480 bottom of core, 6473-6480 middle of core, 6473-6480 top of core, 6464-6470 bottom of core, 6464-6470 middle of core, 6464-6470 top of core) contain nothing that is significant and very little at all. A few crinoid stems, fragments of Fenestrellina, and a Sulcoretepora. The sample 6462-6464 contains Fusulina and is of Pennsylvanian age. In the conversation between Mr. Lee and me concerning this sample, Mr. Lee said that it was almost certainly misplaced. I would think the same, as there are no other Pennsylvanian samples in the entire core. The next 13 samples, ranging from 6293 to 6114 (6291-6293 with intermediate samples to 6114-6119), contain no fossils so far as ascertained. Core 6051-6065 (samples 1-5) contain fossils in sample 1, which is the top, and in samples 2 and 5, as follows: Worthenopora spinosa, fragments of a Spirifer related to S. keokuk and S. washingtonensis, a finely costate Spirifer (fragment) possibly S. lateralis, and Rhipidomella aff. ...
TY - JOUR. T1 - Quantitative footprinting analysis of the chromomycin A3-DNA interaction. AU - Stankus, Allison. AU - Goodisman, Jerry. AU - Dabrowiak, James C.. PY - 1992. Y1 - 1992. N2 - Chromomycin A3 (CHR) binding to the duplex d(CAAGTCTGGCCATCAGTC)· d(GACTGATGGCCAGACTTG) has been studied using quantitative footprinting methods. Previous NMR studies indicated CHR binds as a dimer in the minor groove. Analysis of autoradiographic spot intensities derived from DNase I cleavage of the 18-mer in the presence of various amounts of CHR revealed that the drug binds as a dimer to the sequence 5′-TGGCCA-3′, 3′-ACCGGT-5′ in the 18-mer with a binding constant of (2.7 ± 1.4) × 107 M-1. Footprinting and fluorescence data indicate that the dimerization constant for the drug in solution is ∼ 105 M-1. Since it has been suggested that CHR binding alters DNA to the A configuration, quantitative footprinting studies using dimethyl sulfate, which alkylates at N-7 of guanine in the major groove, ...
The cancer-cell-cytotoxicity-guided fractionation of the acetone extracts of two cultured marine-derived Streptomyces strains belonging to the MAR4 group yielded six new napyradiomycins, compounds A-F (1-6), together with three known compounds, napyradiomycins B2-B4 (7-9). Napyradiomycins 1-4 are new members of the napyradiomycin C-type meroterpenoids, which possess a linear monoterpene bridge between C-7 and C-10a. Compound 4 has an additional tetrahydropyran ring fused to the phenol moiety. Compounds 5-9 are related to the napyradiomycin B-type meroterpenoids. The structures of all new compounds were assigned by interpretation of 1D and 2D NMR, MS, and other spectroscopic data. The relative configurations were assigned based upon interpretation of ROESY 2D NMR experiments. The cytotoxicity of 1-9 against the human colon carcinoma cell line HCT-116 and their antibacterial activities against methicillin-resistant Staphylococcus aureus (MRSA) are presented.. ...
TY - JOUR. T1 - In silico, spectroscopic, and biological insights on annelated pyrrolo[3,2-e]pyrimidines with antiproliferative activity. AU - Terenzi, Alessio. AU - Barone, Giampaolo. AU - Gennaro, Giuseppe. AU - Martorana, Annamaria. AU - Almerico, Anna Maria. AU - Gentile, Carla. AU - Lauria, Antonino. PY - 2014. Y1 - 2014. N2 - The in silico COMPARE analysis was performed on 8-[3-(piperidino)propyl]-4,10-dimethyl-9-phenyl-6-(methylsulfanyl)-3,4-dihydropyrimido[1,2-c]pyrrolo[3,2-e]pyrimidin-2(8H)-one, a compound with promising antiproliferative activity, previously synthetized and screened against a panel of 60 human tumor cell lines. The results evidenced that this compound matches the biological properties of Chromomycin A3 and Actinomycin D, known drugs with high DNA binding affinity. Prompted by such results, a thorough spectroscopic investigation of its DNA aqueous solutions was performed, with the aim to verify its DNA-binding properties. DNA groove-binding interaction was assigned by ...
1EKH: A high-resolution structure of a DNA-chromomycin-Co(II) complex determined from pseudocontact shifts in nuclear magnetic resonance.
Ilan E.Z, Torres M.R, Prudhomme J., Le Roch K., Jensen PR, Fenical W. 2013. Farnesides A and B, sesquiterpenoid nucleoside ethers from a marine-derived Streptomyces sp., strain CNT-372 from Fiji. Journal of Natural Products. 76:1815-1818. ...
15112992] Biosynthesis of the antitumor chromomycin A3 in Streptomyces griseus: analysis of the gene cluster and rational design of novel chromomycin analogs. (Chem Biol. , 2004 ...
Ketopremithramycins and ketomithramycins, four new aureolic acid-type compounds obtained upon inactivation of two genes involved in the biosynthesis of the deoxysugar moieties of the antitumor drug mithramycin by Streptomyces argillaceus, reveal novel insights into post-PKS tailoring steps of the mithramycin biosynthetic pathway.(J. Am. Chem. Soc.) [2002] ...
TY - JOUR. T1 - Synthesis and DNA-binding properties of bisdiazoliumylporphyrins. AU - Tjahjono, Daryono H.. AU - Yamamoto, Tomoko. AU - Ichimoto, Sumito. AU - Yoshioka, Naoki. AU - Inoue, Hidenari. PY - 2000. Y1 - 2000. N2 - A pair of 5,15-bisdiazoliumylporphyrins with only two meso-substituents, 5,15-bis(1,3-dimethylimidazolium-2-yl)-porphyrin 1b and 5,15-bis(1,2-dimethylpyrazolium-4-yl)porphyrin 2b, has been synthesized. The crowded porphyrin 1b intercalates between base pairs of DNA to stabilize the duplex DNA to thermal denaturation due to its high affinity for DNA. The binding of porphyrin 1b to DNA is an exothermic interaction and enthalpically driven. Porphyrin 1b interacts more strongly with poly(dA-dT)2 than with poly(dG-dC)2, and the interaction is more favorable or selective to AT-rich sites than to GC-rich sites.. AB - A pair of 5,15-bisdiazoliumylporphyrins with only two meso-substituents, 5,15-bis(1,3-dimethylimidazolium-2-yl)-porphyrin 1b and ...
PER VRETBLAD; Biospecific Affinity Chromatography of Sweet-Potato β-Amylase. Biochem Soc Trans 1 December 1974; 2 (6): 1327-1328. doi: https://doi.org/10.1042/bst0021327. Download citation file:. ...
Define mithramycin. mithramycin synonyms, mithramycin pronunciation, mithramycin translation, English dictionary definition of mithramycin. Noun 1. mithramycin - an antineoplastic drug used to treat cancer of the testes Mithracin antineoplastic antibiotic - an antibiotic drug used as an...
The contributions from the secondary structure of the transcriptional activator protein C of bacteriophage Mu to its specific DNA binding and the influence of various factors, viz ., electrolytes, and minor groove and major groove binders on this protein-DNA interaction have been addressed . Circular dichroism (CD) spectral results suggest that, in the absence of Mg2+, C protein exhibits a j3-pleated sheetlike structure and Mg2+ changes the conformation to a more a-helical structure which could provide specific geometrical constraints complementary to those of DNA helix. Thus, Mg 2+ acts as a cofactor for the binding of the C protein to its specific site in DNA by inducing conformational changes in the protein. Competitive binding studies with minor and major groove binding drugs, viz ., distamycin A and methyl green, respectively, and the DMS footprinting data indicate that the C protein recognizes the major groove of DNA during complex formation . Further, upon major groove binding, C protein ...
Plicamycin (INN, also known as mithramycin; trade name Mithracin) is an antineoplastic antibiotic produced by Streptomyces plicatus. It is an RNA synthesis inhibitor. The manufacturer discontinued production in 2000. Several different structures are currently reported in different places all with the same chromomycin core, but with different stereochemistry in the glycoside chain, a 1999 study has re-investigated the compound and proposed a revised structure. Plicamycin has been used in the treatment of testicular cancer, Pagets disease of bone, and, rarely, the management of hypercalcemia. Plicamycin has been tested in chronic myeloid leukemia. Plicamycin is currently used in multiple areas of research, including cancer cell apoptosis and as a metastasis inhibitor. One elucidated pathway shows it interacts by cross-binding chromatin GC-rich promoter motifs, thereby inhibiting gene transcription. Mithramycin A. Fermentek. Wohlert, S. E.; Künzel, E.; Machinek, R.; Méndez, C.; Salas, J. A.; ...
Development of industrial producers of antibacterial (ristomycin, linkomycin, apramycin, tobramycin, heliomycin, eremomycin) and antitumor (daunorubycin, carminomycin, bleomycin, olivomycin, bruneomycin) ...
The structure of the synthetic deoxyoctamer d(GGIGCTCC) has been determined by single crystal X-ray diffraction techniques to a resolution of 1.7A. The sequence crystallises in space group P6(1), with unit cell dimensions a = b = 45.07, c = 45.49A. The refinement converged with a crystallographic residual R = 0.14 and the location of 81 solvent molecules. The octamer forms an A-DNA duplex with 6 Watson-Crick (G.C) base pairs and 2 inosine-thymine (I.T) pairs. Refinement of the structure shows it to be essentially isomorphous with that reported for d(GGGGCTCC) with the mispairs adopting a wobble conformation. Conformational parameters and base stacking interactions are compared to those for the native duplex d(GGGGCCCC) and other similar sequences. A rationale for the apparent increased crystal packing efficiency and lattice stability of the I.T octamer is given. Refined crystal structure of an octanucleotide duplex with I.T. mismatched base pairs.,Cruse WB, Aymani J, Kennard O, Brown T, Jack ...
Various approaches are used to study the chromosomal makeup of cells. Traditional cytogenetic methods are based on the analysis of mitotic cells fixed onto slides to analyze their chromosomal composition (karyotype) by microscopy. This approach can be combined with FISH to detect specific sequences on morphologically distinct individual chromosomes. Disadvantages of this type of microscopic analysis are the amount of time and labor required to acquire and analyze typically less than a hundred cells. As a result, the statistical power of this type of analysis is limited. An alternative to traditional cytogenetic methods is flow karyotyping (1,2) a method to analyze chromosomes in suspension by flow cytometry. For bivariate flow karyotyping, the DNA composition of specific chromosomes in suspension is measured based on the DNA-specific dyes Hoechst 33258 and chromomycin A3 (3,4). In our protocol, we combine flow karyotyping and FISH to analyze repetitive DNA in individual chromosomes by flow ...
Poly(ADP-ribose)polymerase (PARP-1)은 세포 핵 내에서 가장 많은 단백질 중의 하나로서 DNA가 손상되었을 때 그 활성이 나타나며 세포 사멸에 깊이 관여한다. PARP-1을 억제하면 세포 사멸 과정이 necrosis에서 apoptosis로 바뀌게 되어 주변 세포 조직에 손상을 유발하지 않으며 항암제 내성에 의한 부작용도 줄일 수 있게 된다. 전 세계적으로 DNA-binding drug을 이용한 항암 치료에 PARP-1 inhibitor를 병용 투여하거나 PARP-1 inhibitor를 단독으로 투여하여 암을 치료하는 방법이 널리 연구되고 있다. 본 연구에서는 2-phenylquinazolin-4(3H)-one 핵을 가진 화합물로서 PARP-1을 억제하여 단독으로 항암 치료에 쓰일 수 있는 약물을 개발하고자 하였다. 2-(4-(4-(substituted)-sulfonyl)piperazine-1-carbonyl)phenyl)quinazolin-4(3H)-one (5-1~5-22)과 benzamide N에 치환체를 붙인 물질 6-1, 6-2, 7-1, 7-2, 8-1 및 8-2를 합성하였다. 합성한 ...
Thank you for your interest in spreading the word about Biochemical Society Transactions.. NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.. ...
SWISS-MODEL Repository entry for B3GZ69 (KTHY_ACTP7), Thymidylate kinase. Actinobacillus pleuropneumoniae serotype 7 (strain AP76)
Radiation therapy is performed both as part of inpatient care and on an outpatient basis. If the cancer cells are shown to have receptors for hormones, hormone therapy may be used to slow their growth. This includes the use of progestins to block the growth of endometrial cells and estrogen-blocking or binding drugs in combination with progesterone.. ...
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Despite the critical roles of excited states in protein functions, they remain intractable for most structural studies because of their notably low populations. Chemical shifts for
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Rita Moreno News. Find breaking news, commentary, and archival information about Rita Moreno From The tribunedigital-orlandosentinel
Molecular Cloning, also known as Maniatis, has served as the foundation of technical expertise in labs worldwide for 30 years. No other manual has been so popular, or so influential.
The pathogenic fungus Fonsecaea pedrosoi constitutively produces the pigment melanin, an important virulence factor in fungi. Melanin is incorporated in the cell wall structure and provides chemical and physical protection for the fungus. We evaluated the production of nitric oxide (NO) in macrophages, the oxidative burst and the inducible nitric oxide synthase (i-NOS) activity in interactions between activated murine macrophages and F. pedrosoi. Experiments were carried out with or without tricyclazole (TC) treatment, a selective inhibitor of the dihydroxynaphthalene (DHN)-melanin biosynthesis pathway in F. pedrosoi. The paramagnetisms of melanin and the TC-melanin were analysed by electron spin resonance. The fungal growth responses to H2O2 and to S-nitroso-N-acetylpenicillamine (SNAP), a nitric oxide donor, were also evaluated. Melanised F. pedrosoi cells were more resistant to both H2O2 and NO. Nitrite was not detected in the supernatant of macrophages incubated with melanised fungal cells. However,
TY - JOUR. T1 - Immunological cross reaction between calf and Drosophila histones. AU - Bustin, M.. AU - Reeder, R. H.. AU - McKnight, S. L.. PY - 1977. Y1 - 1977. UR - http://www.scopus.com/inward/record.url?scp=0017387756&partnerID=8YFLogxK. UR - http://www.scopus.com/inward/citedby.url?scp=0017387756&partnerID=8YFLogxK. M3 - Article. C2 - 856814. AN - SCOPUS:0017387756. VL - 252. SP - 3099. EP - 3101. JO - Journal of Biological Chemistry. JF - Journal of Biological Chemistry. SN - 0021-9258. IS - 9. ER - ...
Online obituary for Rita Levi-Montalcini. Read Rita Levi-Montalcinis life story, offer tributes/condolences, send flowers or create a Rita Levi-Montalcini online memorial.
Hossain, M.Y., M.A. Hossen, F. Nawer, D. Khatun, M.N.U. Pramanik, M.F. Parvin and K. Yahya, 2017. New maximum size records and length-weight relationship for two species, Corica soborna (Hamilton, 1822) and Mystus bleekeri (Day, 1877), from the Ganges River (NW Bangladesh). J. Appl. Ichthyol. 33:661-662 ...
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Just a quick update for those of you wondering how Rita is doing... The answer is: better. She is having a good time seeing lots of other kitties at Barbs house and has perked up a good deal. She is still having trouble passing stool, and needs nightly massages to help her do that. Very…
Euphorbia Linnaeus, 1753 (Euphorbiaceae) is one of the most diverse and complex genera among the angiosperms, showing a huge diversity in morphologic traits and ecologic patterns. In order to improve the knowledge of the karyotype organization of Euphorbia hirta (2n = 18) and E. hyssopifolia (2n = 12), cytogenetic studies were performed by means of conventional staining with Giemsa, genome size estimations with flow cytometry, heterochromatin differentiation with chromomycin A3 (CMA) and 4,6-diamidino-2-phenylindole (DAPI) and Giemsa C-banding, fluorescent in situ hybridization (FISH) with 45S and 5S rDNA probes, and impregnation with silver nitrate (AgNO3). Our results revealed small metacentric chromosomes, CMA+/DAPI0 heterochromatin in the pericentromeric regions of all chromosomes and CMA+/DAPI− in the distal part of chromosome arms carriers of nucleolar organizing regions (NORs). The DNA content measurements revealed small genomes for both species: E. hirta with 2C = 0.77 pg and E. hyssopifolia
The reproduction of twenty-seven strains of Fonsecaea pedrosoiwas studied. The denticulate type (asexual reproduction) showed three morphological variations: medium-size, long and sessile forms....
Online obituary for Rita Levi-Montalcini. Read Rita Levi-Montalcinis life story, offer tributes/condolences, send flowers or create a Rita Levi-Montalcini online memorial.
RITA, also referred to as NSC 652287, is a trycyclic thiophene derivative that binds to MDM2, disrupting the MDM2-p53 complex and subsequently activating p53 and inducing apoptosis.
Rita Sahat iu Ora (born 26 November 1990), better known as Rita Ora, is a British singer-songwriter and actress. 95-106 Capital FM Summertime Ball 2012 at
Discover 124 Rita Rd, New Castle, DE 19720 - single family residence with 1,332 sq. ft., 3 beds, 1 bath. Get the latest property info at RealtyTrac - 195307027.
MODERN CLASSIC A full-length two-hander with a single setting has to be made of really special stuff to avoid outstaying its welcome. Jeremy Raisons production of Educating Rita for the Citz manages to keep its audience onside - just - but in spite of…
Description from Flora of China. Lycopodioides Boehmer, nom. rej.; Selaginoides Séguier; Stachygynandrum P. Beauvois ex Mirbel, nom. rej.. Morphological characters and geographic distribution are the same as those of the family.. Five subgenera are recognized. In a molecular phylogeny (Korall et al., Int. J. Pl. Sci. 160: 585-594. 1999), the isophyllous Selaginella subg. Selaginella (absent from China) and S. subg. Tetragonostachys Jermy are monophyletic, but the anisophyllous S. subg. Stachygynandrum Warburg and S. subg. Heterostachys Warburg are not monophyletic.. Six uncertain taxa, not included in the following key, are listed at the end of the account. Uncertain taxa Selaginella effusa Alston var. dulongjiangensis W. M. Chu (Fl. Yunnan. 20: 718. 2006), described from Gongshan, Yunnan. Selaginella jugorum Handel-Mazzetti (Symb. Sin. 6: 8. 1929; Lycopodioides jugorum (Handel-Mazzetti) H. S. Kung), described from NW Yunnan. Selaginella monospora Spring var. ciliolata W. M. Chu (Fl. Yunnan. ...
DNA topoisomerase I (EC 5.99.1.2) [1,2,3,4] is one of the two types of enzyme that catalyze the interconversion of topological DNA isomers. Type I topoisomerases act by catalyzing the transient breakage of DNA, one strand at a time, and the subsequent rejoining of the strands. When a prokaryotic type I topoisomerase breaks a DNA backbone bond, it simultaneously forms a protein-DNA link where the hydroxyl group of a tyrosine residue is joined to a 5-phosphate on DNA, at one end of the enzyme-severed DNA strand. Prokaryotic organisms, such as Escherichia coli, have two type I topoisomerase isozymes: topoisomerase I (gene topA) and topoisomerase III (gene topB). Eukaroytes also contain homologs of prokaryotic topoisomerase III. There are a number of conserved residues in the region around the active site tyrosine; we used this region as a signature pattern. Last update: December 2004 / Pattern and text revised. ...
IMMUNOPATHOGENESIS OF PCM The establishment of the disease, its spread and severity depend on factors inherent to the fungus itself, as its virulence, antigenic composition, environmental conditions and especially those factors related to the hosts ability to develop an effective immune response. With regard to the latter aspect, we can consider that P. brasiliensis synthesizes metabolic antigens which interact with the host immune system, causing an immunological response that is both highly complex and multifactorial.14 Clinical and experimental studies have suggested an interaction between specific and nonspecific defense mechanisms in determining resistance to P. brasiliensis.11, 24 P. brasiliensis presents a complex antigenic structure, with epitopes that are related to pathogenicity. The main antigenic component of P. brasiliensis is a surface glycoprotein of fungal wall with 43 kDa (gp43), an immunodominant antigen associated with virulence factor and/or escape by which the fungus evades ...
The CC-1065 and duocarmycin family of compounds are ultrapotent antitumour antibiotics which demonstrate activity in the picomolar range. These agents exert their biological effect through a sequence selective alkylation at the N3 position of adenine resulting in apoptosis. Despite the potential of this family to exert themselves as successful chemotherapeutic agents, a lack of clinical success has been observed for these compounds. This has been attributed to a lack of selectivity resulting in off-target side effects and toxicity. For this reason, research now focuses on ways in which these alkylating agents could realise their potential using tumour specific, targeted delivery strategies.. Herein, we investigate the use of a duocarmycin SA analogue, functionalised for solid phasesynthesis, in the design of conjugates for targeted delivery to cancerous tissue via the Thomsen-Friedenreich antigen (T-antigen). This antigen is overexpressed in 90% of primary human carcinomas, yet is cryptic in ...
Dr. Rita Sattler is a researcher at Barrow Neurological Institute. Her work focuses on synaptic biology in health and disease. Read more.
See what Rita Somogyiné Bejczi (ritasomogyinbejczi) has discovered on Pinterest, the worlds biggest collection of everybodys favorite things.
How excited was Rita Ora about being cast in this months film adaptation of the mega-bestselling softcore-porn novel Fifty Shades of Grey? Just ask
VetDepot.com - VetDepot is Americas trusted source for discount pet meds from leading brands including Frontline Plus, Dasuquin, Greenies and more. Shop online now.
DJ Fresh jest pierwszym artystą, którego kawałek nagrany w stylu dub step (Louder) dotarł na szczyt oficjalnej angielskiej listy przebojów. Po wydaniu Hot Right Now do tego wielkiego sukcesu może dopisać kolejny. Numer jeden w UK z utworem drum & bass ...
Chromomycins are promising anticancer natural products from actinomycetes. This study is aimed to create an efficient strain ... Optimization of the culture conditions further increased the titers of chromomycins A3 and A2 respectively to 145.1 ± 15.3 and ... The resulting engineered strain showed the highest production titers of chromomycins by a strain of Streptomyces, providing an ... Either overexpression of SrcmRI or disruption of SrcmRII turned on the biosynthetic pathway of chromomycins. The production ...
Mithramycin and chromomycins are the most representative members of the family, mithramycin being used as a chemotherapeutic ...
To the best of our knowledge, the effects of chromomycins A2 (1) and A3 (2) on TRAIL resistance-overcoming activity, and on the ... led to the isolation of chromomycins A2 (1) and A3 (2). 1 and 2 showed potent cytotoxicity against the human gastric ... To the best of our knowledge, the effects of chromomycins A2 (1) and A3 (2) on TRAIL resistance-overcoming activity, and on the ... Chromomycins A2 and A3 from Marine Actinomycetes with TRAIL Resistance-Overcoming and Wnt Signal Inhibitory Activities. ...
Chromomycins: Dactinomycin and Plicamycin. *Miscellaneous: Mitomycin and Bleomycin.. Daunorubicin (liposomal) is the drug ...
Chromomycins: Dactinomycin and Plicamycin. *Miscellaneous: Mitomycin and Bleomycin.. Note: We strongly encourage you to talk ...
Chromomycins: Dactinomycin and Plicamycin. *Miscellaneous: Mitomycin and Bleomycin.. Note: We strongly encourage you to talk ...
strain AP19-2 producing chromomycins. J Microbiol 45:499-504Google Scholar ...
Toume, K., Tsukahara, K., Ito, H., Arai, M. A., Ishibashi, M. (2014). Chromomycins A2 and A3 from marine actinomycetes with ... and chromomycins A2 and A3 have shown inhibitory effects on the β-catenin signaling in cancer cells; however, their detailed ...
Examples include anthracyclines, chromomycins and mitomycins.. HOW YOU FEEL WHEN UNDERGOING THE DIFFERENT PARTS OF CHEMOTHERAPY ...
Examples include anthracyclines, chromomycins and mitomycins.. HOW YOU FEEL WHEN UNDERGOING THE DIFFERENT PARTS OF CHEMOTHERAPY ...
Anti-neoplastic antibiotics include, but are not limited to, the aclacinomycins, bleomycins, chromomycins, mitomycins, and the ...
08/01/1969 - "Effect of olivomycins, chromomycins and mithramycin on leukemia La of mice of strain C57B1].". ...
When the second component is an antibiotic it may be selected from the group consisting of: anthracyclines and chromomycins. In ...
... chromomycins, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin, epirubicin, esorubicin, ...
... chromomycins, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin, epirubicin, esorubicin, ...
Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins. FRONTIERS IN CHEMISTRY 8 n. p. MAR 3 2020. Artigo ... Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins Texto completo Autor(es):. Mostrar menos -. Sahm, Bianca ... Here, we adopt a targeted approach based on a reverse-affinity procedure to identify the ability of chromomycins A(5) (CA(5)) ...
Structures and properties of the sugars obtained from the chromomycins Tetrahedron. 22: 2785-2799. 1. ...
Cactinomycin, Carubicin, Carzinophilin, Chromomycins, Dactinomycin, Daunorubicin, 6- Diazo-5-OXO-Leucine, Doxorubicin, ...
... chromomycins, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, doxorubicin, epirubicin, esorubicin, ...
Here, we adopt a targeted approach based on a reverse-affinity procedure to identify the ability of chromomycins A5 (CA5) and ...
... and optimized the culture conditions to increase the titer of chromomycins. The production of emodin nowadays mostly relies on ... The engineered Streptomyces roseiscleroticus strain constructed in this work showed higher titers of chromomycins than ... The engineered Streptomyces roseiscleroticus strain constructed in this work showed higher titers of chromomycins than ... and optimized the culture conditions to increase the titer of chromomycins. The production of emodin nowadays mostly relies on ...
Hadimani, M. B., MacDonough, M. T., Ghatak, A., Strecker, T. E., Lopez, R., Sriram, M., Nguyen, B. L., Hall, J. J., Kessler, R. J., Shirali, A. R., Liu, L., Garner, C. M., Pettit, G., Hamel, E., Chaplin, D. J., Mason, R. P., Trawick, M. L. & Pinney, K. G., Sep 27 2013, In : Journal of Natural Products. 76, 9, p. 1668-1678 11 p.. Research output: Contribution to journal › Article ...
Chromomycins Actinomycin D Mithramycin A Other Mitomycin C Bleomycin Plant Alkaloids Vinca alkaloids Vincristine Vinorelbine ...
The chromomycins. It is available as raw material. - UW-Madison School of Pharmacy (@UWMadPharmacy) July 5, 2019. Draft ...
Chromomycins (0) * Galactosides (0) * Glucosides (0) * Hemoglobin A, Glycosylated (0) * Iridoid Glycosides (0) ...
Dextrorotatory Chromomycins from the Marine Streptomyces sp. Associated to Palythoa caribaeorum. Journal of the Brazilian ... Targeting the Oncogenic TBX2 Transcription Factor With Chromomycins. FRONTIERS IN CHEMISTRY, v. 8, MAR 3 2020. Web of Science ...
The full structures of three chromomycins, A2, A3, and A4. Miyamoto M. et al. Tet. Lett. 1966, 6, 545. ...
A mixture of several closely related glycosidic antibiotics obtained from Actinomyces (or Streptomyces) olivoreticuli. They are used as fluorescent dyes that bind to DNA and prevent both RNA and protein synthesis and are also used as antineoplastic agents ...
Lin CI, Whang EE, Donner DB, Jiang X, Price BD, Carothers AM, Delaine T, Leffler H, Nilsson UJ, Nose V, Moore FD, Ruan DT. Galectin-3 targeted therapy with a small molecule inhibitor activates apoptosis and enhances both chemosensitivity and radiosensitivity in papillary thyroid cancer. Mol Cancer Res. 2009 Oct; 7(10):1655-62 ...
Effect of olivomycins, chromomycins, and mithramycin on leukemia La of mice of strain C57B1. Antibiotiki(Moscow) 1969;14:721- ...
  • Mithramycin and chromomycins are the most representative members of the family, mithramycin being used as a chemotherapeutic agent for the treatment of several cancer diseases. (nih.gov)
  • Optimization of the culture conditions further increased the titers of chromomycins A 3 and A 2 respectively to 145.1 ± 15.3 and 158.3 ± 15.4 mg/L in liquid fermentation. (biomedcentral.com)
  • The resulting engineered strain showed the highest production titers of chromomycins by a strain of Streptomyces , providing an efficient way to produce these pharmaceutically valuable molecules. (biomedcentral.com)
  • The engineered Streptomyces roseiscleroticus strain constructed in this work showed higher titers of chromomycins than previously reported, which was achieved by characterizing and engineering the chromomycin biosynthetic gene cluster. (usu.edu)
  • Mithramycin and chromomycins are the most representative members of the family, mithramycin being used as a chemotherapeutic agent for the treatment of several cancer diseases. (nih.gov)
  • Cytotoxicity-guided fractionation of the CKK1019 strain culture broth, which exhibited cytotoxicity, led to the isolation of chromomycins A 2 ( 1 ) and A 3 ( 2 ). (mdpi.com)
  • An engineered strain was then constructed with both SrcmRII disruption and SrcmRI overexpression, which produced chromomycins A 3 and A 2 in R5 agar at 69.4 ± 7.6 and 81.7 ± 7.2 mg/L, respectively. (biomedcentral.com)
  • Toume K, Tsukahara K, Ito H, Arai MA, Ishibashi M. Chromomycins A 2 and A 3 from Marine Actinomycetes with TRAIL Resistance-Overcoming and Wnt Signal Inhibitory Activities. (mdpi.com)
  • Chromomycins are promising anticancer natural products from actinomycetes. (biomedcentral.com)
  • I activated the polyketide biosynthetic pathway by engineering two regulatory genes, and optimized the culture conditions to increase the titer of chromomycins. (usu.edu)